LOCUS       DAOTHZ010000001        76047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_282_length_76047_cov_7.956535, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000001 DAOTHZ010000000
VERSION     DAOTHZ010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 76047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76047
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_282_length_76047_cov_7.956535"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="P5549_00005"
     CDS             <1..562
                     /locus_tag="P5549_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRW11485.1"
                     /translation="HKGLFGPQGTGGLYIHPDIKVQPLKEGGTGSLSEHLEQPDFMPD
                     MLESGTLNTPGIAGLGAGIAFVSQQGIDRIRQHERELLVQLIDGLQQIKGIRLYGPLD
                     PQRQTAVLAFNVEGLDCGELSSQLDYEYGIITRAGMHCAPLAHQTIGSDISGSCRLSP
                     GYFNTRAEIDHVLQAIEKLSGSNEAG"
     gene            714..1481
                     /locus_tag="P5549_00010"
     CDS             714..1481
                     /locus_tag="P5549_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRW11486.1"
                     /translation="MNIQGAVALVTGGASGLGAAAVEMLIEKGAQVVVADINAEKGRE
                     FVAGLGDNAIYVQDNVTSTADNQAAVEAAIATYGKINILLNAAGVASAAKIIGKDGPT
                     PLEWFKLVIDINLAGAFDVMRLAAWAMSKNPPDDKGEKGVIINVASVAAFEGQMGQAA
                     YSASKAGVVGMTLPIARDLSRDGIRVNTIAPGLFDTPMMAALQQAARDSLGAQVPFPK
                     RLGEPAEFARLVCHMIENGYLNGETIRLDGGIRMGIK"
     gene            1657..2520
                     /gene="map"
                     /locus_tag="P5549_00015"
     CDS             1657..2520
                     /gene="map"
                     /locus_tag="P5549_00015"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRW11487.1"
                     /translation="MNKINRNDLCWCGSQKKYKKCHLEMDERLARLEEEGLEVPGREL
                     IKTESQIEGIRRACQLSREILDYLEDKIVPGLRTDTIDQWVYDYTMEHGAYPAPLNYR
                     GYPCSVCTSINEVICHGIPSARELRDGDIVNIDVTSILDGYYGDTSRMYLVGDPSPQA
                     RKLVEVARECLYLGIEQVKPFNRIGDIAYAIEQHAKAHGFSVVRDYGGHGVGLQFHEE
                     PFVHHYGARESGMVLVPNMVFTVEPMINAGTYKCRTLEDGWTTLTADGSLSAQWEHTV
                     RVTDDGVEILS"
     gene            2560..4047
                     /locus_tag="P5549_00020"
     CDS             2560..4047
                     /locus_tag="P5549_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRW11488.1"
                     /translation="MKAIKKVMALVIAMAVSCLFCFAPLAVAAGSANDSLTGEYLQQL
                     MNLAQEQYNYDITESELTQGALKGIFSSMDDYTEFFTIEEARQFMETVGGTYSGVGVV
                     LSQTESYVVVTRVFSLSPAEKSGIMIGDKIATIDGKDVLGSSVDEVSSLIRGEEDTQV
                     YVGILRDKSLHNYTLTRKSIKINPINYEIRDGIAYISIDTFNSNTYDYWAEAQNAADE
                     AGVDRIILDLRNNVGGEVDQAIAVARQLVPAGIISTLEFRNPELRDTVYKSYLEKSPY
                     KLVVLVNGMTASASEILAGAIQDTNTGTLVGTKTFGKARVQSVMPVLSPEGYGKYGQK
                     ADVVNVYDLKLAEQMKLGDEDVVGLVKITTGTYLTAAGRMIDGAGLTPDETVADPEVK
                     NGIYVNSIAKLSQTVKPGLNDENLDVYNAEKILKIIGYEIDTPDMTLDSKTFAAIQKF
                     QADQGLYAYGVLDYTTQQALNRQYYQVLPQVDQQYGRALEILQAR"
     gene            4124..5194
                     /locus_tag="P5549_00025"
     CDS             4124..5194
                     /locus_tag="P5549_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRW11489.1"
                     /translation="MHKKLFIPGPVEVSADVLAKMATPMIGHRSKNASELQHRISDKL
                     RQVFYTQEEILLSTSSGSGLMEGAVRSCTLKKAAVFSIGAFGNRWHEMAVANQVPADL
                     FEVEWGQATTPDMVDQALATGQYDVLTITHNETSTGLMNPVEAIAAVVKKYPEIVFCM
                     DAVSSLGGTKIEVDKLGVDICITSTQKALGLPPGLSICSFSAKAIDRARQVPYRGYYL
                     DLLALYEYIQKKDYQYPSTPSLSHMFALDYQLDKILAEGLENRFRRHIEMAEMTRAWA
                     RRYFELFADEKYLSNTLTNIKNTRGIDVADLNKKLGERGFQISNGYGKLKDKTFRIAH
                     MAECTREELQELLEQINDILKL"
     gene            5215..6459
                     /locus_tag="P5549_00030"
     CDS             5215..6459
                     /locus_tag="P5549_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRW11490.1"
                     /translation="MSTIRPFQAIRPPASLAARVAALPYDVMSSEEARQMVTGNPYSF
                     LHVDKAEIDLPSDVDLYDERVYQKARQNLDGMIAEGILQQDGQACFYIYRQIMNGRSQ
                     TGIVACCSMDEYLDNKIKKHEFTRADKEQDRINHVNVCDANTGPIFLTYRNRTDINSI
                     IAQWCTQAPVYEFQSEDGIGHVVWVIDDLQVMADLQHCFRQVEELYIADGHHRCASGV
                     RVGQMRRQQYPNYTGEEEFNYFLAVLFPDQELQIMDYNRVIKDLNGLSRAAFLAAVED
                     KFSITATGASIPYKPQSRHHFGMYLDEQWYELTAREGTIAEDDPVARLDVSILQNNLL
                     GPVLGIADPRTDRRIDFIGGIRGLEELEKRVKEGMAVAFSLYPVTILDLMDIADAGQV
                     MPPKSTWFEPKLRSGLFIHKLT"
     gene            complement(6528..7760)
                     /gene="lysA"
                     /locus_tag="P5549_00035"
     CDS             complement(6528..7760)
                     /gene="lysA"
                     /locus_tag="P5549_00035"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRW11491.1"
                     /translation="MILAYVEKVFGKADVFDLLKEYGSPLYVYDEEILRHRCRELMNL
                     LRYPSFRVNYSAKANTNVELLKIIREEDLDVDAMSPGEMYLEMKAGFTPDRIMFIGNN
                     VSRQEMQFAVDRGIYVSVDSLSQLALLGEVNPGGEVCLRLNPVIGAGHHDKVITGGKK
                     AKFGIAIEDLQQAQEIAERYQLTITGLNQHIGSLFLETEEYVAAAAQLLTIASSLPQV
                     KMLDFGGGMGIPYQHTSEPRLDLEKLGRELDEIIQTWVKNNRPITVRIEPGRYVVAEC
                     GAILGTVTAVKHNYHTDFIGCDIGFNTLIRPTMYDSYHEISVVPAHSRAYAEYDGAVY
                     LVGQICESGDILAHDRTLPVCREGDALIVHDAGAYGFAMASTYNSRPLPAEVLIQSDG
                     TVRLIRPAQTLQDLWPEK"
     gene            complement(7858..9045)
                     /locus_tag="P5549_00040"
     CDS             complement(7858..9045)
                     /locus_tag="P5549_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRW11492.1"
                     /translation="MTTLKKSFTPLLLFGLSSLMMGMYAGLYDPSFNNYLAHVHNVSE
                     VARGGLEFPRELPGFLVVFLFTALVFLADTRIAAFSALLVSLALWGQGFLAPNMAAVV
                     VWMLIWSTGAHLFMSVSPAIGLRLSTSGEEGRRLGQLGSLESLGTLLGIVIVYWGASR
                     FHFSFATIFAISGTCALIASIGLFLIKPQPIRKPSRHLVIKKRYTLFYLLNIVFGARK
                     QIFMTFAPWVLIKVYQADVATFAVLGFIGTVLNLVFRPWLGRVIDAWGERTIIMLESL
                     TLIIICGLYAFAEMWFPARIAVIIIMACYITDQLLFSVRIARTTYLHKIVETPDDLGA
                     TLSMGLSMDHAVSMLIPLGGGILWARYGYISVFLVAACLAVVNLWLATFIPIRQKNHS
                     ELA"
     gene            complement(9220..11790)
                     /locus_tag="P5549_00045"
     CDS             complement(9220..11790)
                     /locus_tag="P5549_00045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW11493.1"
                     /translation="MTFVELFFTYIPLVALCSYCLLMFFFCIAQKDRLIRAFIPVLGA
                     MILWTVSAVFMSIKLYPGVLFWDRVMIIGAVMVTYLLYYFVSVFTNSLRILPTIFWGI
                     GTFAFIIINLKGYIVADAGVITNTVMLWGREFEVRKFTYSIGTLAPFIYIFALAMIVS
                     TLLKAKRSVSHIKNSPSAGSVRLINNGIAIMFIGNVCNLIPVLGQFPIDLLACFINAF
                     MLMIAIYKYRMVELRFMITRGLVYSAFVVSLTIAYIFIVFFIENHMLTRYEKIIPYFT
                     TLVALAVAITLQPLYRLSSRLVDKLFYKAEYTQRQALRQFSNRISNNLDLNSIARELI
                     EAVQLALNASHVLLLQKNDEHKTYRVFATSSQLFQPDLEISFDNPVIKWMNKNNSGLN
                     RDQLYYQPFFKSMWEQEKSKLHNTGIELIIPIKVRDNIIALLMLTNRKNNVAYTLDDL
                     DLLTYLGASSAVAFENARLYSRSQSDAITDSLTKLYNHRYFCQALPESVDKIGASEVS
                     LLMIDLDLFKLYNDLYGHFEGDRALEMVANILKRIIGDKGTICRYGGEEFTVILPYHD
                     ARKAYELAEKIRTEIEASFFNVNDVTQRFLTASIGICTYPQAAPNTEELLKRADLAMY
                     TAKNQGKNQTVIYTPRIITKQNVSSCDTEDLFQPNYTSTIYALAAAIDAKDHYTFGHS
                     QRVANYVTILANEMSLDKAHIEVLQEAALLHDIGKLGIPENILTKAGRLTEEEFEIIK
                     KHVEMSITIIKHLPTLNHVIPAVIGHHERWDGKGYPRGLKGENIPFSARCLAIADAFD
                     ALTSNRPYRAGLTINSALAEIKNNLGTQFDPAMANLFIKLVQDGTIKVAKQAEIKSIS
                     "
     gene            complement(11792..13402)
                     /locus_tag="P5549_00050"
     CDS             complement(11792..13402)
                     /locus_tag="P5549_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRW11494.1"
                     /translation="MNLDQLLKDKQYERIWHTYCGFLDFSIDEYMDIQNRLMLEQIEM
                     YSRCELGRRIMKGAKPSSIDEYRQVVPLTRYEDYADLLLLRAASVLPAKPLLWIETTW
                     EGAKNPIKVAPYTESMIKCHKSSFITCLILSTSTQKGQFSLRGGENFLYGMAPLPYLT
                     GLAPLIMQDEISVNWLPSEKEAVSLSFSGRNKLGFKLGMQQGVDLFFGLSSVIAKIGE
                     QFASGGGTGDKINPLNNSLKMNYRMLKAWTNRLEEKVPIRPKDIWNLKGLICAGTDSA
                     GYKAKIEANWGIRPLEIFGGTETTCMATETWSKNGLVFFPDVCFYEFIPWPEVEKNLE
                     DPDYVPRTYLMDELQEGMKYELVISNLKGGAFARYRVGDIFKCISLYNEQDDLRLPQF
                     VYLDREPNLIDIAGFTRISEATINEALTLSKLDIHDWCALKQFDESNTAFMHLYVEVS
                     PEGIHYGLTRDIITEHLAIYFRYIDSDYKDLKSLLGIEPLQITILPTGTIDRYQELFG
                     KRPQRMNPSHFEMLELMKIARGSNITGV"
     gene            complement(13438..14079)
                     /locus_tag="P5549_00055"
     CDS             complement(13438..14079)
                     /locus_tag="P5549_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRW11495.1"
                     /translation="MNLAQQKHKEKSRRYFDKSAFNYELTWDGQYCQQMYAQVLEKIS
                     EQPFESLLDVGCGSGVMLAILGKEQPHMKACGIDLSEQMIKQAQIKLGTNADLRVGDA
                     DNLPWNDDIFDLAVCNAAFHHYPNPIQSLKEMYRVLKPGGRLVIADPWWPKKLRQAIN
                     YYLQSPFNTKGDVRIYSPEEMEQMLDASGFKHIDWELVARTYFIVTAVTESYQ"
     gene            complement(14080..14652)
                     /locus_tag="P5549_00060"
     CDS             complement(14080..14652)
                     /locus_tag="P5549_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11496.1"
                     /translation="MSFIFAGSLAFICFAIFDLNKIRFISKNMNILVVLGSFILAFST
                     MAIIISPHSIRISTLWQWLALILAILVLLLMIYTIFGAVSFKKTYLETQKGNTVVTTG
                     MYALCRHPGVLWFFLFYCLSGLAFNNQLLLIAAIIWTLLDIVYVYIQDRWIFPIILED
                     YHIYQQQVPFIIPNLTSLQHSLASLLGKGD"
     gene            complement(14980..16137)
                     /locus_tag="P5549_00065"
     CDS             complement(14980..16137)
                     /locus_tag="P5549_00065"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HRW11497.1"
                     /translation="MPLKRAVAAAAREIGFADIGFTGIDPFSSQQQLLQERAEYYRWT
                     SDKGLDLLEGTVPSLLMPEAKSMIVVLDNYYQGSFPASMVGKFGRCYLDDDRITKDRL
                     AIKIKQLRNYLRENGIQSKVANDLPQRLAAARAGLGTFGKNNFFYASRTARQSSWVVA
                     VAILTDGEFAPDEPSIAVTCPQWCRNACIASCPTGAILGPNKIDPQRCISYLTYYGEE
                     LTPRELREPMGMWVYGCDRCQSVCPRNEPWLAQELPKNERVLQKSVWFELPRLLHMDK
                     EYFKQNIQPHMFYMSSRDIWRWKMNVARVMGNSCDRQYVSALISAYPENDDERVQAMI
                     VWALGKLGGASSIAALRQFAQDASGIVKAEIAWALASAAETDGNIFNSATG"
     gene            complement(16333..16767)
                     /locus_tag="P5549_00070"
     CDS             complement(16333..16767)
                     /locus_tag="P5549_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRW11498.1"
                     /translation="MAVDLKNLERILTMHNIKPSHHRLRILRYLTEHKNHPTVDMIYR
                     ELLDEIPTLSKTTLYNTLNLFVERGIINMLNLEENENRYDADISLHAHFQCAHCSKIY
                     DIHIEVTQLGISGLDGFEVNESRVYFKGVCPICIENRNKQIN"
     gene            16951..18261
                     /locus_tag="P5549_00075"
     CDS             16951..18261
                     /locus_tag="P5549_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRW11499.1"
                     /translation="MKDWPEYIRFIFDKLLAHGYEVFAVGGCVRDMIAGIKPQDYDMV
                     CSASLEQISQIFSRTLTVGARYGTVIVLIDRHPVELSTYRKVELNQKVTALEMDLAGR
                     DFSINAMAMDVNGIIYDPWGGQADLKAGLIRATGDRAEVLFADDPLRMMRAIRFSVTY
                     DYQIAESTCAALLKLNHLLAGIAWERIREELNRILTSDRPAQGIRKLQTGGLMAYIIP
                     ELEVMVGFDQRNFRHNKDLFEHSLAVLEGVPGQIDVRLAALLHDIGKPACFTIDENGV
                     GHFYNHHQAGMEMTRRILERLKYDQQTITNVSLLVGSHMTRYAKLRNSSLKQLIKQVG
                     EDKLDSLYTLQRADIMASAPPFDFSELDKMQQAIDFILDTNQPLSIKDLAVSGTDLLT
                     IGYAPGPFLGETLQRLLEFVLEDPSQNQRELLLHMASVWKDQQL"
     gene            complement(18296..18733)
                     /locus_tag="P5549_00080"
     CDS             complement(18296..18733)
                     /locus_tag="P5549_00080"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRW11500.1"
                     /translation="MNDHYRRLEHIYLCAPYNVALYDETTIRISEGRAEISTRVSPRH
                     HHGAGALHGSGYFRLLDDAAFFAVNSTVTDAMVYTVSFNTQFLRPVQSGIITALGKVK
                     HSSSNLFIGESSLYNEKGQLIALGRGDFMKSKIDLHTIPGFDE"
     gene            18992..19465
                     /locus_tag="P5549_00085"
     CDS             18992..19465
                     /locus_tag="P5549_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRW11501.1"
                     /translation="MAEDRQFVVFKLVHDDQVSEYAVPISNVQEIIPLPEPIRLPQVP
                     DFIEGIINLRGTIIPIIDLKKRFELGNSVEVKDKRSIIIDLAGQIVGIIVDEVNEVLR
                     LSEDKIEAPPAVISGIASEYLIGVGKLADRLLIILDVEKVFNEWEKDVLKSACSA"
     gene            19533..19901
                     /gene="acpS"
                     /locus_tag="P5549_00090"
     CDS             19533..19901
                     /gene="acpS"
                     /locus_tag="P5549_00090"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRW11502.1"
                     /translation="MRIGIDIVDIDRVEAAVKRTPRFLSRVFTVGELDYCLAKRNPYP
                     SLAVRFAAKEAFRKIDQYFCQGIRFQDVEVAVREGGRPELVLHAQALARTKEIGIDRW
                     EMSLAHSRNQAIAAIIAYGE"
     gene            19906..21459
                     /locus_tag="P5549_00095"
     CDS             19906..21459
                     /locus_tag="P5549_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRW11503.1"
                     /translation="MKLITADEMKAIDRRASKDYLIPSIVLMENAGMRTTEKIVELLD
                     GVQERCIIILVGRGNNGGDGLVIARHLLNAGAQVDVFMCAEADQLSGDAAINYAILDR
                     MKGRIHPLRQEADRERFIMALLQADMVVDALYGIGFRGSLSEFEAEIARLVNQSQSMV
                     LAVDIPSGLEADTGQVNGIAFKADYTVTFALPKRGMILGRDRQHVGQLTVADISIPGA
                     LLKDEALQLQIITKEMVSPYFKPRNSNTHKGSYGHTLVIGGSMGMSGAVVMTAWAALR
                     CGAGLATLALPDKIVSAAGLVPEIMSRGLPSTSQGAIAAEACPILENLLGTVSVCALG
                     PGMSRYGEGHAVVKTVLEKAGIPVLIDADGLNALQGDTEILRDRQVPIVITPHPGEMA
                     RLTGRSVEEIQNQRIDISRDYASEWGVTIVLKGHHTIVAHPSGQVFINVNGNPGMATA
                     GSGDVLSGIIAGLMAQGMKPTEAAVAGVYLHGWAGDEAARHQGQRSLTATDLIHRMSD
                     VINSLENNG"
     gene            21502..21951
                     /locus_tag="P5549_00100"
     CDS             21502..21951
                     /locus_tag="P5549_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRW11504.1"
                     /translation="MLAKDIMTRDVITVRAEEEVEKVAQLLLDNKISGVPVVDENYHL
                     VGMVTEKDLMVKATELRVPFYITLFDSIIFLENPIRFKNDLKKYTASQVKDAMTKKVY
                     WVEEDTPVAEVAALMQKENINRVPVLRHKKVMGIITRNDLLKTLVKK"
     gene            21951..23081
                     /gene="alr"
                     /locus_tag="P5549_00105"
     CDS             21951..23081
                     /gene="alr"
                     /locus_tag="P5549_00105"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRW11505.1"
                     /translation="MDNRSTSWAEIDLGALRRNISAIRALLQPDTRLMAVVKANAYGH
                     GVLQVARVCMENGADYLAVANVQEAMELRQANIHAPILVLGMVPEEQAALMSRHDIEA
                     AVADLESAQGYSRQASTVPPLRLHVKLDTGMGRIGFQTDEKTVGEIKTIQQLPNVTLQ
                     GVFSHFAAADAADKEYSRRQTSDFEAFIECLQANNIHIPVKHLANSAAIIDLPETQYN
                     MVRSGIIMYGLYPSDQVKREKLPLEPVMRLKTRIAFLKTLSPGRSVSYCRTFVAPQDT
                     LVATVPIGYADGYSRLLSNRAWAVVRGQKVPLIGNVCMDQCMFDVTGVEGVEKGDEMI
                     LFGRPEDGVTADDLAHITGTINYEIVCTLGQRIPRVYVGQGG"
     gene            23166..23393
                     /locus_tag="P5549_00110"
     CDS             23166..23393
                     /locus_tag="P5549_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRW11506.1"
                     /translation="MPDSKRISITIPVDLLGELDTLCHLEHKNRSEIVRQAIVDYLEE
                     RRKNAMIEQMKKGYLEMAAINLGIAGEDKQN"
     gene            23434..23793
                     /locus_tag="P5549_00115"
     CDS             23434..23793
                     /locus_tag="P5549_00115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRW11507.1"
                     /translation="MMIKRGEIYFAQLNPVIGSEQGGLRPVLVVQNDIGNQYSPTTIV
                     LAITSQINKAKLPTHVELKAETYGLERDSVVLTEQIRTIDKKRLKQRVAVLNDETMEK
                     VDQALGISIELANRINT"
     gene            23958..24638
                     /locus_tag="P5549_00120"
     CDS             23958..24638
                     /locus_tag="P5549_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRW11508.1"
                     /translation="MIGITCNYVQTANLLSLRDAYAESIYMAGGLPLILPVASQQDLC
                     RQYFDLCDGFLFSGGGDADPYWFGQTADRDLGEVSPLRDAFEIQLARMALATGKPALG
                     ICRGCQILSIADGGTILQDIKSKQLHDQKAPRDYPIHDILIESGSLLASIVQSDCIRV
                     NSFHHQAIDKAGDNLIISAWARDGIAEAVEGRNGFFYLGVQWHPECLQDICSQRLFAA
                     LTEQSKYF"
     gene            24712..25866
                     /locus_tag="P5549_00125"
     CDS             24712..25866
                     /locus_tag="P5549_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRW11509.1"
                     /translation="MNKMYAIRGGKILTMAQPGTVDDAMILIEGERIRQINYNLQPPD
                     GYKVIEAQGKYICPGFIDSHTHLGLEEEIYRIEGDDVNEMTHPVTPQLQAVDGINYFD
                     LGFEDALRGGITRAMVMPGSANILGGQAAFLKTLASRPAEMIYKNPWGLKAALGENPK
                     RVYGADKKSPQTRMASASLLREAFFKAARVIDKLDLEGGEAYAQEPIFKVLRREMPLL
                     LHVHRADDILTALRIMDEFHFDLLIQHGTEAHLVADELCERKVGVFLGPLLVNRAKVE
                     MKSVAFKNARLLAERGVKFSLITDHPVVPIEHLRVCAALAVREGLSEDTALQALTVEP
                     ASFLGVEEELGSIKVGKRADLVIFNGHPLDFRSVVEEVFVDGLLWRNRIE"
     gene            25859..26050
                     /locus_tag="P5549_00130"
     CDS             25859..26050
                     /locus_tag="P5549_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11510.1"
                     /translation="MNRGDLFTVYMEGAMMTVCVIASYQEEYSGEEMVILAVINPDNL
                     VHVSREELDYLFPRSKLLN"
     gene            complement(26053..27972)
                     /locus_tag="P5549_00135"
     CDS             complement(26053..27972)
                     /locus_tag="P5549_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW11511.1"
                     /translation="MILPNMKPKSNDPKTSATDLTFFNDFQGIFENLNVPILVADENL
                     DIIVVNLQYEILVGYSRQELIGKNILDIIKLPENKQLLTKYHAQRMLDPCSVPSKYEI
                     VIIDKNDNVQYLLLNVSLIPESKYTLASMQNITEQRQAEAKVKASENYYQTIFDTTGT
                     ATLLLEEDMSISRVNRECERIIGYTNEELTSMKWPQIIHPDFVEQMKQFHQLRRALPN
                     AAPFKYKTRMINKQNQPREGLMAVELIPGTKTSVATFIDLTDFNRIDRSLKAISACNQ
                     IMLHAQTEKELLHDICQTIVDIGDYSMCWVGYLQEDAKQTVLPVAWAGKAENYVENLK
                     ISLTDKRRRAGPTGMSIITGKPVIVRDIKNEKDRPWLKSALQQGFKSSMSIPLLNQNK
                     PFGALCIYADETGIFNGEEQNLLKEMADNLDYAIITLRTRQSHSQTLRKTQLYVEKMR
                     VLMRQAANALGAVIEKRDPYTAGHQSNVAAMAVAIAREMGLSDEQLAGLYAAASLHDI
                     GKITVPIEILSKPGILSEPELAIVKSHSQVGYEILKEIEFPWPVADIVLQHHEKIDGS
                     GYPNGLRGQEIMLEARIITVADVVEAMSAHRPYRPTRGLEEAIKEITDNRGILYDPEV
                     VDICLQLIDRDFADH"
     gene            28175..29524
                     /locus_tag="P5549_00140"
     CDS             28175..29524
                     /locus_tag="P5549_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRW11512.1"
                     /translation="MKRFYLICCLAMAFWLAGAVPLSAGIGPIIKINGQTRTYEPPCQ
                     IVAGRTMIPIRYVIEDPALRGAVDWNETKAEVSVWCRDKHFVFRIGSHTASVDNQNLV
                     LDVAPYIYQNRTYIPLRFLAEQLSARVEWNASAREVSVHFATQKDRVFAYYYYHSFDE
                     FKHNAESITDVALRWFETNAEGDLFYEYQDDYEQILQFAREKSIKTHASVVFMDKDGL
                     HALLSDSVKRKYLIAQLLEEVKRNNYDGVNIDFEFLGQADRDSFVQFLRDLKQGLGRD
                     KELSVALFACTKPQSWLAGYDYAAIGGIADQVVVMAYDYHYKTSPAGPVAPLWWVEDV
                     VSYLKTIIPAEKLLLGLPTYGYDWGEGIDGTTITAAKLYSLQGKYKLTESFDKASMSP
                     SYSYIDNNGVRHQIWLENKASLQAKLQVAQEYKLAGVSFWRIGNGFTELYELLEEWQ"
     gene            complement(29581..30486)
                     /locus_tag="P5549_00145"
     CDS             complement(29581..30486)
                     /locus_tag="P5549_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRW11513.1"
                     /translation="MELRQLKIFVMVAEEGSFTRAAQKMGYAQSNLTTQVRLLEEEFN
                     TKLFERLGKRIALTADGETLLLHAKKLLQYADEIKDTLTSSPAPNGILNIGIAESLCI
                     FKLPALLKEYSRLYPQVKLVIRQGTPLDFQRWLCDNTIDVAFSLDSIIRDEALLVRIL
                     CEEPMIIVANREHPRAAQGDFALADIGTESFIFTERDCSYRSVIEKHLAESKIQPLSS
                     YEFDSIEAIKQFVLSGLGIALLPYAAVEKELAAGNMVDLRLRQPKIEMYTQIVCHKNK
                     WMSPALTALLDLVNTHFKGEIIPLG"
     gene            30651..31535
                     /gene="dapA"
                     /locus_tag="P5549_00150"
     CDS             30651..31535
                     /gene="dapA"
                     /locus_tag="P5549_00150"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRW11514.1"
                     /translation="MGKPLLFAGSAVAIITPFKDELIDYEKLAELIEFQIENNSDAIV
                     ICGTTGEASTIPDQDHIAAIQFTVERVKKRLPVIAGTGSNHTSHGMDLSRAAEAAGAD
                     AILSVTPYYNKTTQNGLYAHFKAIADSIRIPVILYNVPTRTNLNLNPETVRELSLIDN
                     IVAIKECNMAQAGDVINLCGEDFTLYSGDDNMVVPMLSLGGKGVISTTANLIPADFHQ
                     MVSLYLQGDVEGARKIQLQTLNLIKALFIEVSPIPIKAALNMLGMKVGNCRMPLVEMS
                     EANRAILEQEMKSYGLLK"
     gene            31754..32443
                     /locus_tag="P5549_00155"
     CDS             31754..32443
                     /locus_tag="P5549_00155"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRW11515.1"
                     /translation="MMIEQQGLFGEEEPAEKQAGNPVVQESLADIPFLPGIKFEEAYP
                     DVKDLDMLGKKALGCRGCRLRETCQQVVFSAGSADAKIIFIGEGPGSDEDEQGVPFVG
                     RAGQLLNQILAAAELKREEVYITNVVKCRPPGNRLPNPDEVKVCRSYLEAEIRIIKPH
                     IIVCLGAMATQTVVDPKARIGQLRGRWLQRNGIRIMPTYHPAALLRNPSYKRPVWEDF
                     KLIRDVYKTLT"
     gene            32469..32948
                     /gene="tsaE"
                     /locus_tag="P5549_00160"
     CDS             32469..32948
                     /gene="tsaE"
                     /locus_tag="P5549_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRW11516.1"
                     /translation="MELIINNEEEMLVLGKKIAAQLEPDDVIYLTGELGVGKTTLVRG
                     IARARGYNGRVTSPTFTLMNIYATDPPLYHFDFYRLESGDTVDLGLEDYLEKQGISLI
                     EWPNLTDSSLPDEALLITITLLYDDYELARKVEIAARGGKYEKKIKGLMASVHPGHR"
     gene            32926..33642
                     /gene="tsaB"
                     /locus_tag="P5549_00165"
     CDS             32926..33642
                     /gene="tsaB"
                     /locus_tag="P5549_00165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRW11517.1"
                     /translation="MYILAIDSATPVAGLALLNDEKVIREDFINFKKTHSETLMPGVD
                     RIMHDCELTLDQLGAIAVTIGPGSFTGLRIGLAAAKGLSMSSGKPLIGISTLDVLAGN
                     LVFSDNLVCPLLNARKQEVYTAFYEAGGAYPRRLTQDMACSPQEAAARARELAHRLGK
                     PNITLLGDGFYPYEEYYREYFREDLQIVPGPLMLPRAAVLGSLAWQRARQNDFDDTFA
                     LRPVYVRLSEAEYRLGKGAL"
     gene            33643..34095
                     /gene="rimI"
                     /locus_tag="P5549_00170"
     CDS             33643..34095
                     /gene="rimI"
                     /locus_tag="P5549_00170"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRW11518.1"
                     /translation="MLNTRYMIRKMAENDLDKVMIIEQESFSLPWSRESYLGELKNDF
                     ANYLVCDDGGEIAAYGGVWVVFEEAHITNVAVAIRYRGQGVGSDLMKELEKIARSKHA
                     TRILLEVRPSNQVALAMYDKLGFIQTGVRKAYYSDNGEDALIMTKYLF"
     gene            34113..35123
                     /gene="tsaD"
                     /locus_tag="P5549_00175"
     CDS             34113..35123
                     /gene="tsaD"
                     /locus_tag="P5549_00175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRW11519.1"
                     /translation="MKETLILGIESSCDETAAAIVKNGREVLASVINSQISIHQQFGG
                     VVPEVASRKHIENIASVVETAFLEAGLRHDEIDAVAVTNRPGLIGALLVGLSFAKAFA
                     YGLGKPLIAVDHLQGHIWANFLEHPDISFPAICLVVSGGHTSLLLMEGPGQSRLLGET
                     RDDAAGEAFDKVARFLGLGYPGGPAIQKAAQQGTAGIYNLPRVFLDREDYEFSFSGLK
                     TAAMNLWIKQSRKGEARVEDMAAEFQAALVEVLVEKTIRAAKEYKAPSILMAGGVAAN
                     QLLRQLMTIRAQQEGRQLFYPSLGLCTDNAAMIAGKAYLDYVADNFAPLHVNAHPGQN
                     SL"
     gene            35262..36026
                     /gene="lgt"
                     /locus_tag="P5549_00180"
     CDS             35262..36026
                     /gene="lgt"
                     /locus_tag="P5549_00180"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRW11520.1"
                     /translation="MRVHPVLLEFGRIKIYSWGFMLAIAVIVSIIGLTKMFKREGYDP
                     DSVIDSVIILVLGGILGSRLLYIFAYQWQELLTDPAAVLSLKDGIQGLVWYGAVILDV
                     PLFIWYLRRKKYPLWNILDMFAPFVALGYAIVRVGCFLNGCCYGEVTSSVCGVVFPYV
                     DSFTRYPTQLFSTFMNLLLFGLLLWYYPRRKYDGEVFVFYFMGYSIYRFLIEFMRVSQ
                     INFGPFTMSQIISMAIFIIALLLHIYKIKQVAAQKE"
     gene            36046..36630
                     /locus_tag="P5549_00185"
     CDS             36046..36630
                     /locus_tag="P5549_00185"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRW11521.1"
                     /translation="MHINLQGSKAALRSEMKAQRGLLDMKQIEQASLAIARRLNDLLP
                     VQKAKTIMGYAAMKKEVNLDAFYEQQLQLGKTIFLPRVEGDQIKPVKWNGWKDIKVSS
                     FGVREPLGEEFPAAEIDVVLVPGLAFDPGGFRVGYGRGYYDRFLPGLRSDAFKCGICY
                     EFQVVDSTFPHDRDVPVHWIVTEKSELVVNWDFF"
     gene            complement(36702..37640)
                     /locus_tag="P5549_00190"
     CDS             complement(36702..37640)
                     /locus_tag="P5549_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRW11522.1"
                     /translation="MDYITNFILVLFVIIIAVSAIKIIRQATVGIVERLGKYHKTAEQ
                     GINVIIPFIDRFRAIVDMREQVVSFAPQPVITKDNVTMMIDTIVYYQVTDPFRYTYEI
                     ANPIMAIDNLSATTLRNIVGDLELDQTLTSRDLVNTKLRTILDEATDKWGIKVNRVEL
                     KNILPPQDIQTAMEKQMRAEREKRQAILLAEGQKTATILEAEGEKQSAILRAEAVKEA
                     AVRTAEGSRQAKILEAEGEAQAILNVQRAFADSLTMIKDAGADDKVLAIKSLEAIKQL
                     GEGQATKLIIPADLAGVGGVFAALKEVVLDKPEEQQ"
     gene            complement(37685..38116)
                     /locus_tag="P5549_00195"
     CDS             complement(37685..38116)
                     /locus_tag="P5549_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRW11523.1"
                     /translation="MTTAQWVIVAVVCGSIEIFTMGLWFLWLALAALLVAIGVRLEWL
                     TTLQWQLLLFAFFTLVFIIFTRPLAVKLFKTKDTVSNVKALVGQHGISTTALRPLQYG
                     QVKVNGEIWTAISDSEIEADRRIEVTDIDGVKLIVRPTEQE"
     gene            38368..38541
                     /locus_tag="P5549_00200"
     CDS             38368..38541
                     /locus_tag="P5549_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11524.1"
                     /translation="METKEIIEKIKELAPQGKISCADARQLAEKTNIEYGQIGKLCDE
                     AGVKIYGCALGCF"
     gene            38598..39578
                     /gene="moaA"
                     /locus_tag="P5549_00205"
     CDS             38598..39578
                     /gene="moaA"
                     /locus_tag="P5549_00205"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRW11525.1"
                     /translation="MQDGYGREINYLRISVTDRCNLRCQYCMPETGINHVGHEDILSL
                     EEISRIVRVAASIGIRKVRLTGGEPLVRRNLSQLVHYIHEIKEIDDIALTTNGVLFAA
                     LGDELKTAGLNRINFSLDSLVAEKFRFITRSGNLPIVLQGIEKALDLGLHPIKINTVL
                     IKGFNDDEIMDFVALALKYPLHVRFIEFMPIGDLLFWRPDRLMTSHEIRQRIEKEYVL
                     EETYKVPGSGPASYYKIQGGLGSIGFISPMSNHFCARCNRLRMTADGKLRACLYEQGE
                     TDLRDALKRSSDDEELRQLFLATLRSKPQRHHMQSGWGDDNRRKMYQIGG"
     gene            39583..40065
                     /gene="moaC"
                     /locus_tag="P5549_00210"
     CDS             39583..40065
                     /gene="moaC"
                     /locus_tag="P5549_00210"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRW11526.1"
                     /translation="MGLSHINEKGYARMVDVSDKQDTVRVARAQAIVCMQPETLAMIR
                     EGTIKKGDVLSVAQVAGIMGAKQTAQIIPMCHPIFLSGVDLDFCMDEVRCSIIITSQA
                     KTTGKTGVEMEALTAVTVAALTIYDMCKALDRWMEINDIRLLEKSGGKSGHLINSKAE
                     "
     gene            40089..40535
                     /locus_tag="P5549_00215"
     CDS             40089..40535
                     /locus_tag="P5549_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRW11527.1"
                     /translation="MIQGRLYSICISSERGQLKNEVDEAQLVQGYGIENDSHAGDWGR
                     QVTCLAKESVEKVNQEKNLQIGPGQFAENLLIEGLDFPSVGVGGKLKIGAEAILEVSQ
                     IGKEDHPSIVTETFGVSLLPYEGLFCRVIQGGPIKKGDEVEVLQNV"
     gene            40528..41019
                     /locus_tag="P5549_00220"
     CDS             40528..41019
                     /locus_tag="P5549_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HRW11528.1"
                     /translation="MYNCGILVLSDKGSQGEREDLSGMEIKKTMSGKDYVFTYYEIIP
                     DDEEMIREKLILACDDLKLDLLLTSGGTGFSKRDVTPEATLSVIEKRVPGIPEAIRYH
                     GLQNTPKAMLSRAVAGIRGNTLIINLPGSVKGVRESLEAILPALDHGLDILIGSAAEC
                     GQG"
     gene            41206..41499
                     /locus_tag="P5549_00225"
     CDS             41206..41499
                     /locus_tag="P5549_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRW11529.1"
                     /translation="MKIQPLGDRVVVKVAEVKEEKTKSGLYVPDTAKEKPQEAEVMAV
                     GPGALNDKGERVSIDLAVGDKIIFSKYGGMEIKVDGEDYLILSAERDVLAKLV"
     gene            41539..43173
                     /gene="groL"
                     /locus_tag="P5549_00230"
     CDS             41539..43173
                     /gene="groL"
                     /locus_tag="P5549_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817317.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRW11530.1"
                     /translation="MSKEIKFGEEARRALEKGVDTLANTVKVTLGPKGRNVVLDRKFG
                     SPTITNDGVTIARDIDLDDPWENLGCQLVKEVAIKTNDVAGDGTTTATVLAQAMIKEG
                     LKNVAAGANPMIMRRGIEKAVQAAIKEIHAISQPVETKESIAQVASISASDPDIGSLI
                     ADAMEKVGRDGVITVEESQTMGTQLEVVEGMSFDRGYISPYMVTDSEKMQAVLDDPFV
                     LLTDKKISAISEILPVLEKVVQTGKPLLIIAEEIEGEALATLVVNKLRGTFTCVAVKA
                     PAFGERRKAMLEDIAILTNGRVVSEETGSKMENVDLTMLGKAAKIVVKKEETIIVDGA
                     GSEADVKGRIGIIKKQLEETESEYDKEKLQERMAKLSGGVAVIQVGAATETELKEKKH
                     RIEDALAATKAAVEEGIVSGGGTALINAMVAIDDVLAEADELTGVKLVKKALEEPLRQ
                     IANNAGVEGSVVVEKVKAAEHGIGFNALTNVYENMIAAGIIDPAKVTRSAIQNAASIA
                     AMVLTTEAIVSEKPNPNENAAMGGGMGGMGGMGGMM"
     gene            complement(43328..44737)
                     /gene="glnA"
                     /locus_tag="P5549_00235"
     CDS             complement(43328..44737)
                     /gene="glnA"
                     /locus_tag="P5549_00235"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRW11531.1"
                     /translation="MEYCAENSIQMIDFKMIDLMGRWRHLSIPTARFKPDTLVYGIGF
                     DGSNYGFAPVENSDMVFIPDVSTAVHEPFTDVPTLSMIGDVFIITQPENRRFDQDPRS
                     IAFKAEEYMKSTGIADEMRIGPEYEFHVFDHVSYECSPNHTGFTIDAEQAIWNSGDNE
                     FQNQGYKIPHKGGYHIAPPQDILYDLRSRMCVLMEENNIPVKYHHHEVGGPGQIEIEV
                     EFAGMREMADKTMLAKYLIKNLAFQEGKTVTFMPKPLFAEAGNGMHVHMHLFKDGEPL
                     FYDANGYSQLSKTALYFIGGLLKHAPAVLAFTNPSTNSYKRLVPGYEAPVNLCFATAN
                     RSAVIRVPAYAKSPYQKRFEFRSSDATCNPYLAFSAILMAGLDGIKEKIDPTAEGFGP
                     YDVNLYNLPKEEQAKIKSLPKSLDEALDALEKDHDFLLAGDVFPQRLIDIWIENKRKE
                     ARKVNEIPHPMEFELYYDL"
     gene            44996..45709
                     /locus_tag="P5549_00240"
     CDS             44996..45709
                     /locus_tag="P5549_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11532.1"
                     /translation="MRRIKKTYWLSGIAVILLLALVASAGQIMDYYEKSKLQPDVELQ
                     SALKKMPQLNSFRYRLQSGFTVDNREEVISRVEGETTDGNTHIKGEMVNTPIDIYYID
                     RTIYNWDSFSQKWLVIESGTSNSGELLISELNPLSNFQYKNVQDIKKIGFTKVDGEEC
                     LVVGCKPSLQNQLLESLWKNFEYRLWIDFHNRVIKKAVLTAVNQRNEQTRLKIEAEFF
                     DINKKITIKPPERTARDKK"
     gene            45930..48053
                     /locus_tag="P5549_00245"
     CDS             45930..48053
                     /locus_tag="P5549_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRW11533.1"
                     /translation="MIFAFEPLEGGYSIPMRVVGANIPYEWLIYILMFIPIGFFIYGF
                     YQRIKVWKLAQGEEHRNDQLGRRFFSWLVNSMGQLQLVRKPLAGWMHFMLFWGFMFLL
                     LATATFASWSKIGFPDMTGFLYTLISWLADVGGFFALLGIIVLFGIRYWRKPDRLNDT
                     RSSDGWMLLLIFLILLGGYVLEGMWLAAQMQLSTTIAQLDYERVASPVGWMFAGLFQG
                     MTLDNVLLWHRINWWLHMVLAFLFIGLVPYTKLWHIFTGMIGYFSRDFKPSANRMVYH
                     LEEAEHFGVENIEEFTWKDLLDLDACIRCGRCQENCPAYYTGKTLNPKITLIQAMKKH
                     LDDKVPQLMLKEPVHDELAMTSAAEAKVVDAAEASLIYDVVTPEVLWACTNCRACMEH
                     CPMFIEHLNKITDMRRNLVMWQGDMPGEAQSAFTNMERNYNPWGVGWASRADWMGERG
                     VRDKINLLPEDGRDFEYLLFAGCAVAFDDRYKRVGEALVKLLDKAGVSFGYLGTEEWC
                     CGDSARRLGNEYLYQSLAAHNIETFNQYGVKKIICICPHGYNCLKNEYPQMDGVYEVY
                     HATEFLAGLIVAGKLKPQRMNNIRMSYHDSCFLGRHNGIYDEPRKILQAVGANVQEID
                     KSRRFGFCCGAGGGRMWLEEEAVAGFKRINQSRTEQLLQTAPQTIASNCPFCLTMISD
                     GVKEAGQEEQVKVLDLCEILWNSVE"
     gene            complement(48057..48425)
                     /locus_tag="P5549_00250"
     CDS             complement(48057..48425)
                     /locus_tag="P5549_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRW11534.1"
                     /translation="MKLDHLGIVVSDCDQSERFYSEALGCIPVSRWQNDEIKAVNLQC
                     DSLLIELLEYTNPSQSCTSPGVINHLAFHVDDLADTIERLSKLGAVFENVAPKTLSSH
                     KRIIFLHGPNGERIELVEEK"
     gene            complement(48455..48913)
                     /locus_tag="P5549_00255"
     CDS             complement(48455..48913)
                     /locus_tag="P5549_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRW11535.1"
                     /translation="MGNSKDYISILSMAIENEVEAYEFYLGVSQKVEDPNIKSIFKEL
                     AEQELGHRDLLSSYLNSNVRPLNFGALPDYKVSETVDKPKLSMQMKPVDAIALAMKNE
                     EDAMKMYTDLANSSADAAQKKLFMNLANMEKGHKVQLEDIYTNMAFPEAW"
     gene            49104..50903
                     /gene="pepF"
                     /locus_tag="P5549_00260"
     CDS             49104..50903
                     /gene="pepF"
                     /locus_tag="P5549_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HRW11536.1"
                     /translation="MAEQSTRKRNEIDEKYKWDLAKIYVNETLWEEDFQTLQQKIKAI
                     AALQGTIPNSAEALLKVLQTSDEMGRITEKLYVYARMRRDENNADQNYQALFDRAERL
                     SVEASSAAAYIVPEILAIPEADLKNYLQQNKGLAVYRHALDEITRQKEHILSVPEERL
                     LAMAADLSTASSHIFTMLNNADLKFPVIKDEEGHEVELTKGRYGSFMESGNRQVREDA
                     FKGMYSTYRKFMNTLGASLNASIKKDIFYARTRKYPSALTAALDGDNVGVDVYDRLIE
                     AVHNNLEPMYRYMKIRKQQLGVDELHMYDIYVPLAKDYKMTIPYEEACEKMLQSLQPL
                     GQEYLGQVQAGLQEGWIDVYENEGKTSGAYSWGTYDTQPYILMNYDNKLDDLFTLAHE
                     MGHSMHSYYSNHNQPYIYAQYSIFVAEVASTVNESLLIDYLLQHSHDKQEKIYLLNHY
                     LEQFRGTVYRQTMFAEFEKIIHAKAEQGEAITPELMNTVYRDLNKLYYGPEVVLDEEI
                     NIEWARIPHFYSAFYVYKYATGFSAATAIKEAIRREGEVAVEKYLGFLKSGGSDYPII
                     LLQKAGVDLTTPEPVDSALKYFARLVDEFESLL"
     gene            50975..54505
                     /locus_tag="P5549_00265"
     CDS             50975..54505
                     /locus_tag="P5549_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRW11537.1"
                     /translation="MTNSKRESIFDEQIDLILPQLESYNNFDLLIGIPFYGNNCGLHQ
                     VLQSVDQVLQNWIGRRQLIVCIGDANAAEMMHTIENLQLYHPYLAFILPPEASGRGMS
                     IRALLQIARKMEVDLLVFDAPMGTSHGPGINIDWLEGLLTPIQGQYDLVVSSRKRHGG
                     ITSIASLLAMPFLETFYGSRLSEPLGGIYAISHDFVEELVTEAKFWTDTIAGSGIDFW
                     LITRALCWNKQICELYLNENPQSCSLERNSAVFIDVLKTIFACLGRDHALWLPERLVT
                     KVVDILNYKKMPVVESAGVDWDELRQELKKHYQRSEDTIRLILPEENEQQLRQLMTAA
                     TGDYHLSNSAWVSNIYKIFLYYTFSEEVSQKDLLHAAAALFNAKIASCVLELNQFGAG
                     LKAADEFEKDELVSHKLELTRQQLLVEFHKQKPLLSKQWLGRSEQTAPPLVPFGYMEY
                     IPGRPIVVPKRISGKDKHVVNTDELFKKLRKLYEDRFNHFLAEGLGLPAHAEPDRIIA
                     AMEKFMAAAERTVDEMFPGNLNDPDGMEQFVTGLFAMVPHQQMFTINTDLLKEMLIRF
                     PPVNLMIPLGFYSPAELLANIDPRDAVTYANLVDSWSYNDRDLVWLTEQLKPESFGWA
                     QIKPLIVNKSLPEGLWSHTKISNLNRLTARITIKAMEPGKGGKYPKLRYFTSLIRRIC
                     LAEHYSLLFQQNVKERKNVGGKARNSMTAMEKGDDFSAQRIFENSNHRALVEKIGAMA
                     LALQQRGMNEPARIFQMMADGYGLSQVLEDGAFLTCTVWSWASYKGGLNLPTPMTTSI
                     ENRWFNHEFLELLYKELGYDKEEILQTAFRYLQSGKSGHSLLDALLPARPKDIQVVVQ
                     ETTNEPSKTLVRYEGNPLLEPIKEHDWECKYVLNPGAIRIKDKVYLFYRAVGEDNVSH
                     IGLAITDGYRVLERLDTPIFSPATPEEKMGCEDPRLIQIGERIWMLYTAYDGNIAQIA
                     AASISLQDFLARRFTSWKREGLAFKNIWDKDAILFPEKIKDRYILYHRIEPSMWITYM
                     KDLVFPSREKHAIIIGPRPGRMWDSLKIGAGAQPIKTRYGWLLIYHGVDHNYVYRLGV
                     ILVDLENPQRVIYRSPNPVLEPIEDYELGLSGAWVPNVVFTCGAVAGTEKEVLEDEDE
                     ILVYYGAADTSIGMAKATLAEMIPEKFRCR"
     gene            54578..56653
                     /locus_tag="P5549_00270"
     CDS             54578..56653
                     /locus_tag="P5549_00270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRW11538.1"
                     /translation="MKSLVLAEKPSVAREIARVLKCVNKQKGYMEGSQYVVTWALGHL
                     VTLAEPDDYDAKYKQWRLEDLPMLPEKMKLKVIKPTAHQYQVVKSLMNRADINELVIA
                     TDAGREGELVARWIMEQARWKKPFKRLWISSQTDAAIQQGFRQLKPGREYDNLYVAAV
                     CRAEADWLIGLNVTRALSCKYNAQLTAGRVQTPTLAMIVNRENEIQDFQSVDYWTIRA
                     DFGDYYGDWRDRHNQGRIFEAGRAESIVAKVKGQTGTIKDMKKETKAEPPPLAYDLTE
                     LQRDGNRRLGWSAQKTLSILQSLYEVHKVVTYPRTDSRYITRDIVPTLPARLQAMQTG
                     PYVDFIRPLLSQKIQPGKRFVDDSKVTDHHAIIPTEQKLNLNALSVDEKKLYDIIARR
                     FLAVLYPSYKYEQTTITTVVAEEIFLSRGKVVKEAGWRALQAQISEKEENKEEHLPEQ
                     LLQERHQGETRKVGNCRLNKAKTKPPARYTEATLLTAMESPGKYIEDEELREAMKGSG
                     LGTPATRAEIIEKLFNTFYIERQGKEILPTAKGSQLIGLAPPSLRSPELTAQWEQQLT
                     DIARGKSSKGQMITAIREDARQMVSAVAQSSDEYKPTNVSKSKCPACGKNMLLVNTRQ
                     GKMLVCPDRTCGHRQPEKQADTDVFSRKKQGERVNPRLLARYSDQKDLGQSLGDKLKE
                     ALQNKEKKQ"
     gene            57015..59102
                     /locus_tag="P5549_00275"
     CDS             57015..59102
                     /locus_tag="P5549_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW11539.1"
                     /translation="MLITNTETHIMKQLRGIKVHDKCCFIYETEEEWEFYMIPFLKQG
                     LMNGEKCLYHVQRRDEDYLLRCFAKHGIEVEACLASGQLEILKPSFTLPILQTSNMDE
                     IIQTFKSELDQYCAQGFKNLRLTSESISKLMDAEEATSRFLELQLRLNRDVFPYYAIR
                     SITQFHRHEDSPMVLKDAIISHPCIFIGGQIYRNPATISPEIYFQHKDKAWEAEYWLR
                     TQEALIDSEKKYRLIFEHSKDMTTIIDAKTYDIVLISPVHSKLLGYTPEEMIGKNCLE
                     FIHPDQRETMVKEFKSSHTAGKGIYAIRKQDGSYLWTEGKGDIIHNVNGHDQIILFTR
                     DIHDKKLAEDALHRSEQNCRYQLRYLNTLINTMNELCLTYDSDSCLTFVNQRLVEKLG
                     YSAEEMLGKPLLDYVVDDHKQAVAEQLQLRLQGEFGSHEHKFICKDGSELLVELKGSP
                     IYEDEEITGGLVLADDITSQRQMEMDMARLGQMHLVGEMAVSIGHEIRNPMTTVQGFL
                     QILSQNHEFNEHREYFDLMLDELKRANLIISEFISLAKNKVANLQSHNLNSIIQALAP
                     LLMADALNGDKNIKFELSKLPNILLDENEIRQLILNLVRNGLEAMQNGGNVTVKTWQE
                     KNKVMLSVMDEGDGIKEEILEKLGTPFVSTKENGTGLGLAVCYSIISRHQAILQVKSS
                     QTGSSFTVAFNCV"
     gene            complement(59138..60163)
                     /locus_tag="P5549_00280"
     CDS             complement(59138..60163)
                     /locus_tag="P5549_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11540.1"
                     /translation="MPNYKHLYNMTTTTGILQFSKCHHPDPDSGYTLDDNARALLVAL
                     AMGDEGYPLALTYTHYLEHSWQANNTWCNLLLHGDYLSDLNSEDSFGRAFWACSAGSR
                     SKWPLIAGSCTNMLERAVPCLPFLNSARGLAYCLIGLCKGQFPFINAKSINNLIHMLA
                     DRLLALYHNEHSPDWLWFEDKITYCNGIMPHALFAVYASIGNRKYLKIAHASLGFLNH
                     HLFAKGYLNIVGNEGWYVRGGKMPLWDQQPVDAASIVFACHEAYQVTGRNEYKTLLRL
                     AYQWYRGANLNGLPLYDVESGGCFDALTRQGVNLNQGAEAALSLLASDLLLTGYCSDD
                     LPQAQSL"
     gene            complement(60150..61316)
                     /locus_tag="P5549_00285"
     CDS             complement(60150..61316)
                     /locus_tag="P5549_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRW11541.1"
                     /translation="MNIINIGTYLPKQCGIATFSSDLCSSLTAEGHNLNIVAISDHGC
                     NYHYSNQVIFNIRQNQRQDYLKAAAFINAAAADLVILQHEYGIFGGQDGEYVLQLIKL
                     LHKPYILITHTVLPRPSKKQKQILNEACHRAAGIVCMTQRSRNLLIDLYEAPAEIVKV
                     IAHGVPDFVPYAQPELKHQHGLQNHRLISTFGLIGPGKGLEIGLKAIAEVVTDHPDVI
                     YLILGQTHPMLLQQEGEKYRQMLVELMEALGLQRNVLFINKFLSDEELGKYLYMTDIY
                     LSPYPNLDQAVSGTMAFAIGCGRAIVSTAYAYAKEVLKGGRGLLVPNAEPHLLAQGLK
                     KILESQELQKSLQEKAWELGKTWTWSNVGREYLVFFEQVLHLNRLGEEKILAKL"
     gene            complement(61409..62785)
                     /locus_tag="P5549_00290"
     CDS             complement(61409..62785)
                     /locus_tag="P5549_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRW11542.1"
                     /translation="MSSSNIYRQYDIRGIVGQELTDEVMFNIGRAFALYARQHGYRKI
                     LLGRDNRISSPHYRDLLTEALLQSGCEVTDLGMVITPMFYYASRHLEIGAGIMITASH
                     NPGEYNGCKLLLGESTIYGDQITAIKQLAEKKDFPAQSRGSLNNHDIRTAYMQMITEK
                     IKLGPYKPKVVVDCGNGSASPFAPQLFTRLGCETIALYCESDPTFPHHHPDPVKPENL
                     LDLIHLVKQTQADIGIGIDGDGDRLGVVDRDGNMIWGDQLMILFWRDILPRYPHLPSI
                     VEVKCSQSLIDEIRRLGGTPLLYKTGHSLIKAKMKETGAIFTGEMSGHMFFADDYYGF
                     DDALYAAARLLLLLSNSKQTISEMLSDTAKYYATPEIRLPSDDKSKFQLVEQVRQHFT
                     KDHEVIDIDGARIIFPHGWGLVRASNTGPEIICRCEGDSPEVLENITSMVFGYLGSLG
                     LKIPQAAF"
     gene            complement(62806..63852)
                     /locus_tag="P5549_00295"
     CDS             complement(62806..63852)
                     /locus_tag="P5549_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRW11543.1"
                     /translation="MIAVVLAGGRGLRLWPESRYKQPKQLCKLTGSKSMLDETLDRLE
                     TSGVDQLLIITNDNLMADIKSIIQQRPDADKIEILSEPEGRNTAPAIGLSLSRFYPAL
                     ADEVLAIFPADHHVFDISSFCQTLHRAVQAAEQNHLVTIGIEPDHAETGYGYIEKSDR
                     QCNDLEDVFDVLSFREKPDAKTAAMYLDSGRYLWNSGIYVGKVGILAEEFARFLPDIF
                     MHLQSGHEHYLRHYSALPNISIDYGIAEKSQRVTVVPGRFGWSDIGNWNALAELFPYD
                     QQQNYLLGQDILFVDSKNCLVKQNTKTVVLFGVEDVMVVETDDVILVADRHRSQDISQ
                     LVRQIETLERHDLL"
     gene            complement(64142..65305)
                     /locus_tag="P5549_00300"
     CDS             complement(64142..65305)
                     /locus_tag="P5549_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRW11544.1"
                     /translation="MQNINLIATAAFGLESIVANELKKLGYANQKVENGRVDFEAGLE
                     AIPRCNLWLRSADRLLIKAGEFKALSFEELFEQTKALPWDEWLPENAAIPVEGKSIQS
                     KLFSVPDCQAIVKKAVVEKMKQRYPRQWFSEDGAKYKIQVALLKDRVTLTIDTSGAGL
                     HKRGYRKLSAEAPLKETLAAALVSLSRWKADRALIDPMCGSGTIPIEAALIGRNMAPG
                     LGRDFAAENWPQLPAVMWRQAREEAEDAILRHQPLGIMGYDQDVSVLDLARFHAKQAG
                     LQNLLSFQQRDVSQLSSKYKYGYLITNPPYGERLADQNEAEALYRIMGKVFSRLDTWS
                     IYIICSHPRFEELFQRKAHKKRKLYNGRIQCNYFQFFGPKPEQLQGPFALSEQ"
     regulatory      65488..65576
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      65619..65706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            65849..66016
                     /locus_tag="P5549_00305"
     CDS             65849..66016
                     /locus_tag="P5549_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRW11545.1"
                     /translation="MNLMMRLVKEEDGQGMTEYALIIGFVAVALIVTMIAFKDKIADF
                     FDSLSFTAPAA"
     gene            66076..66651
                     /locus_tag="P5549_00310"
     CDS             66076..66651
                     /locus_tag="P5549_00310"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRW11546.1"
                     /translation="MDAGSKNRPCSYIRQGLFNKGGLDLQDIVLAFMLLVCLFTDWRE
                     RKIYNLILIPVFCFGLAFHLVTAGGQGLVHSLAGFLTGLSILIIPFALGGMGAGDVKL
                     LAVIGAVKGPVFVLYTAAGMALAGAMIALAILLYQRRLLSLMAGLARGLQVLLVTRFQ
                     VVAFNLNREKIMFPYGLAIVAGAVGAFWWGG"
     gene            66657..67049
                     /locus_tag="P5549_00315"
     CDS             66657..67049
                     /locus_tag="P5549_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRW11547.1"
                     /translation="MRRRYTNQRGQALVELALILPVLLLLVFGIIEFGRIYASNLMIS
                     NAAREGARAASLGVPDEDIVIIVKDRCAFLDAAKLDIDITPVPPERATGNPVNIDVNY
                     PLEIKTPIISVITGDPCPISARVSMRIE"
     gene            67055..68005
                     /locus_tag="P5549_00320"
     CDS             67055..68005
                     /locus_tag="P5549_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HRW11548.1"
                     /translation="MKEMRTMIGNEKGSAIVLITVLMTVLVAMAAMVTDTGAILLTRS
                     QLQNGVDAAALAALQDLPGDAAQAAATAVSYATADCPPQAEIDTPQILDANRAVLVSA
                     RKTVHYGLARMIGLDAKTVEAQALARLEPITGVSGALPLAVMKDVWIPGQTVTLKGGA
                     QDAIFSGWRGALALGGNGASNYEENLKNGYTSILRLDDLVDVEEGNMSGPTQSGTEYR
                     LGGCPHTPVCTMEHYHTNCPRVGVIPVVEEVLLTNKKGEQTTSRKQVRICGFALFLLS
                     DTQGNGSDGQITGQFIQGIIQGEADPDGPDYGVYRARLVQ"
     gene            68027..68758
                     /gene="cpaB"
                     /locus_tag="P5549_00325"
     CDS             68027..68758
                     /gene="cpaB"
                     /locus_tag="P5549_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRW11549.1"
                     /translation="MKSKLVFILALIFGLIAAYLTFSYLNQMKASMDNREYIRIAVAA
                     RDIPADTTISPEMLQMKAFPSEFRNNLEILDVNEAVGRVALTDFHQGEAILNHHLIKS
                     GERKYGLAYAISSGMRAMAVNVDEASGVAGLIKVGDRVDIIAKLNSEEGSMLPRTVVV
                     LQGVEVLAIGSSLENAKSDNKEKAIVKTITMAVTLEDSLRLKTAVEGGKISLILRSPS
                     DKGFSNPAAFSAIGFQVDVAPLAGS"
     gene            68835..70061
                     /locus_tag="P5549_00330"
     CDS             68835..70061
                     /locus_tag="P5549_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW11550.1"
                     /translation="MDKIRILMASNNQAYREALTSNLSHENDLRIIGEVPDGIQAIKK
                     TEQLLPDIVVIEVDLSSGQEITAVEKITLAHPEVGVIVIGLEDDSESFRKAMMAGARD
                     YFVKEQLSAGELAEAIRRIFLTEKTRLASYASVRRAEQHNKNKVPQIVTVFGAKGGAG
                     KTTLAVNLAVMIAQQSKKRVALLDLDLQFGDVAVYLNLKPRRTMAELVQERNQWDMQL
                     LSNYLVPHSSGIKVMASPLRPEDADLIVPEHVEKILSLLRDHFDYIIIDSPPFFSDTI
                     LAALDMSTQIVLLMSMDLPSVKNLKLSLNLLDSLHHSGKTKLVINRSSMNFGIDIHDV
                     EKTIDFLAAKELPSDGQTVVGAANKGTPFVLSHPQTEVSKALLRITRMIIEDYGYQKD
                     LRERKPKSSLWSKLFG"
     gene            70072..71433
                     /locus_tag="P5549_00335"
     CDS             70072..71433
                     /locus_tag="P5549_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRW11551.1"
                     /translation="MSLSQRLQNKNALGDPENITVPDSKHVCEEKADKYAVLKQRIHK
                     EVIERIDREIKQAELSDAVLTQKTREMVNSILEREAGFLPYSERQMIAAVVSDEVLGL
                     GPLEPLLNDNDITEIMVNGPKQIYVERQGRLELSETRFRDDEHVMHIIDKIVSPIGRR
                     IDESQPMVDARLPDGSRVNAIIPPLALKGPSITIRKFSRTPYNIHDLVRFGSLTLPMA
                     RFLEAAVRGRLNIFISGGTGSGKTTLLNVLASFIPEDERIITVEDAAELQLNQAHVVS
                     LETRAANIEGKGAVTIRDLVRNCLRMRPDRIVVGEVRAGEALDMLQAMNTGHDGSLTT
                     GHANSPRDMLARVETMVLMAGMDLPIRAIRQQITSAVDLIIQQTRLRNGRRVITHITE
                     ILGMEGDTIQMQDIFLYEQRGIDAKGELLGHHFATGITPRLLSKLAAEGITLTTDLFR
                     PEN"
     gene            71452..72414
                     /locus_tag="P5549_00340"
     CDS             71452..72414
                     /locus_tag="P5549_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRW11552.1"
                     /translation="MAAAISVLVFLSGFLFIMGIYQFSTRHRNNIKNRVLSLNERSSD
                     AIEATFVSKQKWGYKNLLRRIGSVFTARSWGRRLEAELSKADMMLRGEEFVGMNILVT
                     IVGGLLGFLLFGGISQALLIGGICMLFPGMLVKHKKKTRLNKLNEQISDSLTVLTNSL
                     RAGYSFQQALELVGREMDGPLALEFRRTLREIHLGNTTEQALINLIKRAQSSDLELML
                     TAVLIQRQIGGNLAEIFDNISNSIKERIRLKGEVKTLTAQGRISGLLIGLLPVGLFAI
                     LILVSPDYIGVLVRENSGRMILGAAVVSEVIGFLMVRKVVDIGL"
     gene            72427..73341
                     /locus_tag="P5549_00345"
     CDS             72427..73341
                     /locus_tag="P5549_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRW11553.1"
                     /translation="MLMIMAVIFLSAFCLTWAVLVRLLATRNLISSRLEELNRYTPQA
                     DREDELQVAFHERVLLPMLKKTSLFTQKYTPQRQRESIETKLLAAGHPFRWTAADFLS
                     VQYSTAVVLAIMAVLASYFSHASSGVQYLATIGGFAVGYMLLSLLLNSIIAKRRTNME
                     KELPDLLDLMVISIEAGLGFDAALQRVVQKSKGPLSQEFNQCLQEMRMGKTRKEALKD
                     LVRRNEEGDLSAFVGAIIQADQLGVSVGNVLRTQADQLRVKRKQKIEEKAMKAPVKML
                     IPMVLFIFPTIFLILLGPSVIQMMETFK"
     gene            73338..73754
                     /locus_tag="P5549_00350"
     CDS             73338..73754
                     /locus_tag="P5549_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRW11554.1"
                     /translation="MNDKQCCRVINHENKRIIAPDIEVAGSFFPRLRGLLGRKTLSAG
                     EGMLLWPCNAIHCFGMRFPIDAIFLDHQYRVTHIKENLTPYSLASDRQASGVLELKAG
                     ESRKHGLNVGDQLIICFAAGAVPDKEARDKEKCGRR"
     gene            complement(73781..75607)
                     /gene="typA"
                     /locus_tag="P5549_00355"
     CDS             complement(73781..75607)
                     /gene="typA"
                     /locus_tag="P5549_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRW11555.1"
                     /translation="MSQKLLRNIAIIAHVDHGKTTLVDKLLKQSGSFRDRQFVPERVM
                     DNNDLERERGITILSKNTSIYYNDVKINIVDTPGHADFGGEVERIVRMVDGVLLLVDA
                     FEGPMPQTRFVLKKALEAGLKPIVVINKIDRPDCRPDEVLDELLDLFIDLGADESQLE
                     FPVAFASAKGGFAKINMNDPNEDMKPLLDMIIDEIPAPAGDGAAPLQLFISSIDYDTY
                     VGRIGVGKILNGTINKNQEAILCNSEGKQDRVKINTLYSYEGLKKVETTSAKAGEIVA
                     VSGIEEITIGDTLCSPDAMEPIAFLHIDEPTISMNFVVNTSPMAGLEGSYVTSRHLRE
                     RLERELLSNVSLRVEEITPDCFKVSGRGELHLSILIETMRREGYEFAVTRPEVILRNM
                     DGKVYEPVERLFIEVPEEFVGTVIEGVGKRKGELMTMLNGTGSVMKLEFLIPARGLIG
                     YRSEFMTDTRGNGILNHTFDNYEPYKGDISTRSRGSLIASESGTAITYGLYNAQERGI
                     LFIDAGTEVYAGMVVGENARNVDITVNVCKRKHLTNTRSSGADDALRLITPVNMSLEK
                     CLEFISDDELLEVTPKSLRLRKTILDKGERDKASGSRSKNRD"
BASE COUNT        20492 a        16724 c        19347 g        19484 t
ORIGIN      
        1 tcacaaaggt ctatttggcc cgcaaggcac cggagggctt tatatccatc cggacatcaa
       61 ggtacagcca ctgaaggaag gcggtactgg ttctttgtca gaacacttgg aacaacctga
      121 tttcatgcct gacatgctgg aaagcggtac cctgaatacg cccggcatag ccggcctggg
      181 agcaggaatt gcctttgtca gccaacaagg gatagatagg attcgtcagc acgaaagaga
      241 attgctggta cagctcattg acggattgca gcaaatcaag ggcattcgcc tgtatggacc
      301 gcttgacccg cagcgccaga cagccgtatt ggcttttaat gtcgagggtt tggactgcgg
      361 ggaattaagc agccagcttg actatgaata tggcattatc accagagcag gcatgcattg
      421 tgctcccttg gcccaccaaa ccattggcag cgacataagc ggcagttgcc gtctcagccc
      481 gggttatttt aatactcgtg cggagattga ccatgtcctg caggcgatag aaaagctgag
      541 tggcagcaat gaagcaggtt gatttaagat ttctcaagag cttttgccaa gctctttttt
      601 ttttggagat taatgttgtg gtaatattaa gaaaattaaa aaaatatgct taaatgcacc
      661 aggaaataca gcttggctgg ggcagcataa aaaagattgg aggagggcaa gaaatgaata
      721 ttcaaggggc cgtagcgttg gtgacaggag gcgcctccgg gttgggggcg gcagcagtgg
      781 aaatgctgat tgaaaaaggg gctcaagttg tggtcgcgga tatcaatgca gaaaagggcc
      841 gcgaatttgt ggccggactt ggagacaatg cgatctatgt tcaggacaat gtgacttcaa
      901 ctgcggataa ccaagcggcg gtagaggccg ccattgctac ctatggaaaa attaatattt
      961 tgctcaatgc cgccggggtg gccagcgcgg caaaaatcat cggcaaagac ggaccaactc
     1021 cgctggaatg gttcaagctg gtcattgata tcaatctggc cggtgcgttt gatgtgatga
     1081 gactggccgc ttgggcaatg agcaagaatc ctcccgatga taaaggagaa aaaggtgtca
     1141 ttatcaatgt agcctccgtt gctgcctttg aaggtcagat ggggcaggcg gcctattctg
     1201 cctccaaagc cggagttgta ggcatgacgc tgccgattgc ccgcgatctt tcccgggatg
     1261 ggattagagt gaataccatc gcgcccggcc tttttgatac tccgatgatg gctgcactgc
     1321 agcaggcagc acgtgattcg ctgggtgccc aggtgccttt ccccaaacgg ctgggagagc
     1381 cggcagaatt tgcccggctg gtttgccata tgattgaaaa cggttatctc aatggggaga
     1441 cgattcgttt ggatggcgga atccggatgg gcatcaagta agaaaagaaa ttaaaacaac
     1501 atggacatgg ccggtcaggg cggatgaacc ctgaccggtt attctttttc agcagaatgc
     1561 aatccgggct acgcaatgcc ggttgatcgt gttaaaatac ctttatatat aaataaaagt
     1621 cagttgtcaa cggttctgag taaggaggca ataaaaatta acaaaattaa tcgcaatgat
     1681 ctctgttggt gcggaagtca aaagaaatac aagaaatgcc atctggaaat ggacgaaagg
     1741 ctggccaggc tggaagagga aggcctggaa gtcccgggcc gggagctgat taaaacagaa
     1801 agccaaatcg aagggatcag aagggcatgt cagctaagca gagaaattct ggattatctg
     1861 gaagacaaga ttgtgccggg tcttcgcact gatacaatcg atcaatgggt ctacgattat
     1921 accatggagc acggcgccta ccctgctcca cttaattatc ggggctaccc ctgcagtgtc
     1981 tgtacctcga tcaacgaggt catctgccat gggattccat cggcacgtga attgcgggat
     2041 ggagatattg taaatattga tgttaccagt attctggacg gctattacgg ggataccagc
     2101 cgcatgtacc tggtgggtga tccttcgccg caagcccgca aattggtcga agttgcccgt
     2161 gaatgtcttt atctggggat tgaacaggtc aagccgttca accggattgg tgatattgcc
     2221 tacgctattg aacaacatgc caaggcgcat ggtttttccg ttgtccggga ttatggaggg
     2281 catggagtcg ggctgcaatt ccatgaagaa ccctttgtcc atcattatgg tgccagagaa
     2341 tcgggcatgg ttttggtccc caacatggtt tttaccgtcg aaccaatgat taatgcgggc
     2401 acgtacaagt gccgtacgct ggaagacggg tggaccactt taaccgctga cgggtcactt
     2461 tcagcgcaat gggaacacac tgtgcgcgtg accgatgatg gagtcgaaat attaagctga
     2521 gcaatgtttt tactgtaaat atttagggag gattaatcaa tgaaagcgat aaaaaaagtg
     2581 atggcgctgg tgatagccat ggccgtgtct tgtttgtttt gctttgctcc gctggcagtg
     2641 gcagccggtt ccgcaaacga ttctttgact ggtgagtacc tgcagcaatt aatgaatttg
     2701 gcccaagagc aatacaacta tgacataacc gaatcagaac tgacccaggg agccctcaag
     2761 ggcatcttca gcagtatgga tgactatacc gaattcttta ccatcgaaga agcccgacag
     2821 tttatggaaa cagtcggcgg aacctacagc ggagttgggg tcgtattaag ccagacggaa
     2881 agctatgtgg tagtaaccag ggtgttttcc ttgtcgccag cggaaaagag cgggatcatg
     2941 atcggtgaca agattgctac gatcgacggc aaagatgtgc tcggatcttc ggtcgatgag
     3001 gtcagcagcc tcattcgcgg agaagaagat acccaggtat atgtcggaat tctgcgcgac
     3061 aagagcctgc acaactatac actgacgcgc aaatcgatta agattaatcc gattaattac
     3121 gaaatccgtg acgggattgc ctatatatcg attgacacat ttaattccaa tacctatgat
     3181 tattgggctg aagcccaaaa cgcagctgat gaagccgggg ttgaccggat cattcttgat
     3241 ttaagaaaca atgttggcgg agaagtggat caggccattg cggttgcccg gcaactggtg
     3301 ccggcaggaa tcatcagcac cctggaattc aggaatccgg agctccgtga tacggtttac
     3361 aaatcctact tggaaaaatc accgtataaa ctggtggtgc tggtcaacgg catgaccgca
     3421 agtgcttcgg aaatactggc gggtgccatc caagatacca atacgggaac cctggtgggg
     3481 accaaaacct ttggcaaagc cagagtacag tccgtcatgc cggtgctcag cccggaaggc
     3541 tatggaaaat atggacagaa agcagatgtg gtgaatgtct atgacctgaa gctggcggag
     3601 cagatgaaac tgggcgatga ggacgttgtg ggactggtta aaataactac aggtacttat
     3661 ttaacagcgg ccggccggat gatcgacggg gcagggctca cccccgatga aacggtggca
     3721 gatccggaag tgaaaaacgg tatttatgtc aacagcatcg ccaaactcag tcagaccgtc
     3781 aaaccgggtc tcaacgatga aaatttggat gtttacaatg cagaaaaaat attaaagatc
     3841 atcggctatg agattgacac gccagatatg acacttgaca gcaaaacctt tgcggccatt
     3901 caaaaattcc aggcagacca gggcttgtat gcatacggag ttttggatta tactacccag
     3961 caagcgctta accgtcagta ttatcaagta ctgccgcaag ttgaccagca atatggccgg
     4021 gctttggaaa ttctgcaggc gcggtaagat acaaaaatca gttgcttttg ataaaataat
     4081 aaaggaccaa gaacgatctg attgaataat ggaggtttga accgtgcata aaaaattgtt
     4141 tattcccggc ccggtggaag tgagtgcgga tgttctggca aaaatggcta cgcccatgat
     4201 cggccacaga agtaaaaatg cctcggaatt acaacaccgt atcagtgata aattaagaca
     4261 ggtattttat acccaggaag aaatcctgct ttcgactagt tccggcagtg gcctgatgga
     4321 aggcgcggtg cgctcctgta ccttaaaaaa agcggctgtg ttctccattg gtgcatttgg
     4381 caaccgctgg cacgaaatgg cggttgccaa tcaagtgccg gccgatcttt ttgaagtgga
     4441 atggggccag gcgacaacac ccgacatggt tgaccaggca ctggcgacgg gtcaatatga
     4501 cgtgctgacc attacccata atgagacttc gaccggtttg atgaatccgg ttgaagcaat
     4561 cgctgcagtc gtgaaaaagt accccgaaat cgtcttttgt atggatgccg taagttccct
     4621 gggaggtacc aaaatcgaag tcgacaaact gggcgttgac atttgcatta cttccaccca
     4681 gaaggccttg ggcctgcccc ccgggttgtc gatctgttcc ttttccgcca aggcgattga
     4741 cagagccagg caggttcctt atcgcggtta ttatttggac ttgcttgcct tgtatgagta
     4801 cattcagaaa aaagactatc agtatccttc gactccgtcg ctttcgcata tgtttgcatt
     4861 ggattatcaa ctggacaaaa tattggcgga aggtttggaa aaccgcttcc gtcgccacat
     4921 cgaaatggcg gaaatgaccc gtgcctgggc acgccggtat tttgaactgt ttgccgacga
     4981 aaagtatttg tccaataccc tgaccaatat aaaaaacact cgcggcatag atgttgccga
     5041 tctaaataaa aaattgggcg agcgcggttt tcaaatttcc aacggttacg gcaaattaaa
     5101 ggacaagacc ttccgcattg cccacatggc tgaatgtacc agggaggaac tgcaggaact
     5161 gctggagcag atcaatgata tcctgaaact ataataaaaa caggaggaaa taccatgtcc
     5221 acaatcagac catttcaggc aattcgtccc ccggcaagtc tggccgcgcg ggttgccgct
     5281 ctgccctatg acgtcatgag cagcgaagaa gccaggcaga tggtaaccgg caatccctat
     5341 tcgtttctgc atgtagataa agccgagatc gatttgccgt cagacgtcga tctctatgac
     5401 gagagagtct atcaaaaagc ccgccagaac ctggatggca tgatcgcaga agggattttg
     5461 cagcaggacg gacaggcatg tttttatatt taccgccaga ttatgaatgg ccgcagtcaa
     5521 accgggatcg tggcctgctg ctcaatggat gagtatctgg acaataagat taagaaacat
     5581 gaatttacac gggctgacaa ggagcaggac aggattaacc atgtgaatgt ttgcgatgcc
     5641 aataccggac cgatcttttt aacctatcgg aatcgtacgg atattaattc gatcattgcc
     5701 caatggtgca cgcaagcccc agtctatgaa tttcagtcgg aagacggcat cgggcatgtc
     5761 gtatgggtga ttgatgacct gcaggtcatg gcggacttgc agcattgctt caggcaggtt
     5821 gaggaactat atatcgctga tggacatcac cgctgcgcat ccggggtacg agttggtcag
     5881 atgagacgcc agcagtaccc caattatact ggagaagaag agtttaatta ttttctggca
     5941 gttttatttc cggatcagga actgcagatc atggactata atcgggtgat caaggacttg
     6001 aacggtttgt cccgggcggc atttttggct gcggttgagg ataaattctc aattactgcc
     6061 accggcgcga gcatccctta caagccgcag agcagacatc attttgggat gtatctcgat
     6121 gagcaatggt atgaattgac ggcccgggaa ggcactattg cggaagatga tccggttgcc
     6181 cgtttggatg tttccatcct gcaaaacaat ctgctgggtc cggtgctggg cattgccgat
     6241 ccccgtaccg acagacgaat cgattttatt ggcgggattc gcggtttgga ggaactggaa
     6301 aaaagggtca aggaaggcat ggcggtggcc ttttctctct atccggtcac cattctggat
     6361 ttgatggata ttgctgatgc cggacaggtg atgccgccca agtcgacctg gttcgaaccc
     6421 aaactgcgca gcgggttgtt tatccataaa ttgacttaaa ccgtgcagat aaaaataaaa
     6481 aaaacctgga cggtccaaga ccgcccaggt ttttttgttt ccagggctta tttttccggc
     6541 cacaaatctt gcagggtttg ggccgggcgg atgagtcgga ctgtaccgtc tgactggatc
     6601 agaacttccg ccggaagcgg ccgggaatta taggtgctgg ccatggcaaa accgtaagcc
     6661 ccggcatcat gaacgatgag agcatcgcct tcacgacaga ccggcagagt tcggtcatgg
     6721 gccaggatat caccgctttc acagatttgt cccaccagat agacggctcc gtcatattct
     6781 gcgtacgccc gggaatgggc gggcacgacg gaaatttcat ggtagctgtc atacatggtt
     6841 ggacggatta acgtattgaa tccaatatca cagccaataa agtcggtgtg ataattatgt
     6901 ttgacagctg tcactgtgcc caagatcgca ccgcattccg ccaccacata gcgccccggt
     6961 tcgattcgga cggtgatggg gcgattgttt tttacccagg tttggataat ctcatccagt
     7021 tcacggccca gtttttccaa gtcaagcctt ggttctgagg tgtgctgata aggaatgccc
     7081 atcccgccgc cgaagtccag catcttcact tgaggcaggg aagatgcgat tgtcagcaac
     7141 tgggcagccg cagctacata ttcctccgtt tccaggaaca atgaaccgat atgctgattt
     7201 agcccggtga tcgtcaactg gtaacgctca gcgatttcct gggcttgctg cagatcttca
     7261 atggcgatgc caaatttggc ttttttgccg ccggtgatga ctttgtcatg atgaccggcg
     7321 ccgatgacag gattcaagcg cagacaaacc tcgccgccgg gattgacttc gcccaacagg
     7381 gccagctggg acaaagaatc caccgaaacg tagatgccgc ggtcaaccgc aaattgcatt
     7441 tcctgccggg agacattgtt gccgataaac ataatccggt ctggcgtaaa gccggctttc
     7501 atttccaggt acatttcccc gggggacatg gcgtcaacgt caaggtcttc ctcacgaatg
     7561 attttcagca attccacatt ggtgttggct ttggccgaat agttcacccg gaaagaaggg
     7621 tatcgcaata ggttcattaa ttcccggcat ctgtgtcgta aaatctcttc gtcgtagaca
     7681 tataaaggac tgccgtattc cttgagcaga tcaaacacat ctgctttgcc aaaaactttt
     7741 tccacatatg ccaagatcaa acactcctcc ttaaaaaagg cccccaaaac agcctataat
     7801 tactctattg taaagtttgt tggggaaaat gacaagacat gcaatatgta tacataatta
     7861 tgcaagctca gaatgattct tttggcggat tgggataaaa gtcgccagcc acaaattgac
     7921 caccgccaag caggcggcta ccagaaatac cgagatatag ccatacctgg cccagagaat
     7981 gccgccgccc aaaggaatta acatagagac ggcatggtcc atgctgagcc ccatggaaag
     8041 ggtagcgccg aggtcatcag gggtttcgac gattttatgg agataggtgg tgcgggcaat
     8101 acggacggaa aagagcagct ggtcggtaat atagcaggcc atgatgatga tgactgcgat
     8161 tcgagccgga aaccacattt ccgcaaatgc ataaagccca caaatgatga tcaacgtaag
     8221 tgattccaac atgatgatgg tcctctctcc ccaggcatca atcaccctgc ccaaccaggg
     8281 gcggaaaacc aggtttaaaa ccgtgccaat aaagcccagc acggcaaagg ttgctacatc
     8341 cgcctgataa accttgatca gaacccaagg ggcgaaggtc ataaagattt gtttgcgcgc
     8401 gccgaagaca atattcagca gataaaagag ggtgtatctt tttttaatga ccaaatggcg
     8461 ggagggttta cggattggct gcggtttaat aagaaaaaga ccgatgctgg caattaaagc
     8521 acaggtaccg gatatggcaa agatagtcgc gaaggaaaaa tgaaaacgag atgcccccca
     8581 atacacgatg acaattccca acaaagttcc cagggactcc agcgaaccca actgtcccaa
     8641 ccgacgacct tcttctccgc tggttgacaa gcgcaggccg atggcagggg agacgctcat
     8701 aaaaagatga gcaccggtgc tccagatcag catccagaca acaaccgccg ccatgttggg
     8761 ggcgagaaac ccttgtcccc acaaggccag actgaccagc aaagcggaaa aggctgcgat
     8821 tctggtgtcg gccaggaaaa ccaaggccgt aaacaaaaag accaccagaa atcccggcag
     8881 ttcacgggga aattccagtc ctccccgggc gacttcgctg acattgtgca catgagccag
     8941 atagttgttg aaggaaggat catataagcc tgcatacatt cccatcatca aagaggatag
     9001 tccaaacagc aggagagggg tgaacgattt ttttaaagtg gtcataaacg taaacctcaa
     9061 tgaattatta ttatcttgtt tattctaaac ttgttgaaca gagatggaaa gaggttttat
     9121 ccattggcaa tcgttgtaaa ggagaacagg tattgactaa tgggagcatg gaggggttca
     9181 gtgcaaatgc ttgccaatgt ttgaaaaggc aaggcaaaat caactgatgc ttttgatctc
     9241 cgcctgtttg gcaactttta tcgtgccatc ctgcaccagt ttaataaata gattggccat
     9301 ggccgggtca aactgggtgc ccagattatt cttgatttcg gccagcgcgc tgttgattgt
     9361 cagacctgcc cgatacgggc gattggaggt taacgcgtca aaggcatcag caatggccag
     9421 acatctggcg gaaaaaggaa tattctctcc tttcagcccg cgcggatatc ctttgccgtc
     9481 ccatctttcg tgatgcccga taaccgctgg gatgacatgg ttcagcgtcg gcagatgttt
     9541 gatgatggtg atggacattt ctacatgctt tttgataatc tcgaattctt cctcggtaag
     9601 cctgccagct ttggtaagga tgttttcggg tatgcccagc ttgccgatat catgcagcaa
     9661 tgccgcttcc tgcaacactt caatgtgcgc tttgtccagc gacatttcgt tggcgagaat
     9721 ggtaacatag tttgctactc gctgtgaatg accaaaggta taatgatcct tggcatcaat
     9781 cgctgctgca agagcataga tggtggaagt atagttgggc tggaataaat cctctgtatc
     9841 acaggaggaa acgttttgct tggtaatgat gcgcggtgta tagataaccg tttggttttt
     9901 cccctggttt ttggcagtat acatagccag atcagcacgt ttcaagagct cttcggtatt
     9961 tggtgcggct tggggatagg tacagatgcc gatactggca gttaggaaac gctgggttac
    10021 atcattcaca ttgaaaaagc tggcttcgat ttcggtacgg attttttccg ccagttcata
    10081 cgcttttcta gcgtcatgat acggtagaat gacggtaaat tcttcgcccc cgtagcggca
    10141 gatggttcct ttgtcgccga tgattcgctt cagaatgttt gctaccattt ccaatgcgcg
    10201 atcaccttcg aaatgaccgt aaagatcgtt gtacagttta aacaaatcca aatcgatcat
    10261 caacaaggaa acttctgatg caccgatctt gtcaacgctt tcaggcagtg cctggcaaaa
    10321 atatctgtga ttgtatagtt tggtcagact gtcggtaatg gcatcggatt gggagcgtga
    10381 atacaaacga gcattttcaa aggcgaccgc ggatgacgca cccaggtaag tgagcaagtc
    10441 cagatcatcc aacgtatagg cgacattgtt tttccggttg gtcagcatta gcaaggcaat
    10501 aatattgtcc ctgactttga tcgggatgat gagttcgatc ccggtgttgt gcagtttgct
    10561 tttttcttgt tcccacatgg atttaaaaaa aggctggtaa tacaattgat ctctgttaag
    10621 accggagtta tttttgttca tccattttat aacgggatta tcgaaggaga tttccagatc
    10681 cggctggaat agttgggatg aagtcgcaaa cactcgatag gttttatgtt catcattttt
    10741 ttgcaggagc aaaacatgcg aagcattcaa agccagttgt acagcttcaa tcaattctct
    10801 ggcgatgctg tttaaatcca aattattgga aatgcggttg ctgaattgtc tcaaagcctg
    10861 ccgttgggta tattcagctt tataaaacaa tttgtcgacc agtcgggatg acaaccggta
    10921 caggggttgc agagtaatgg caacagccaa cgcgaccagc gtggtaaaat agggaatgat
    10981 tttttcgtag cgggtcagca tatggttttc gatgaaaaat acgataaaaa tataagcgat
    11041 cgtaagtgaa acaacaaaag ctgaatatac caatcctcgg gtgatcatga aacgcagttc
    11101 caccatacga tatttgtaaa tggcaatcat aagcataaaa gcgttgataa aacaagccag
    11161 aagatcaatc ggaaactgcc ccaggacagg aattaagtta catacgttgc ctataaacat
    11221 gatagcgata ccgttgttga ttaaccggac gctaccggca gaaggagaat tttttatgtg
    11281 acttacgctg cgcttggctt tcaaaagagt tgagacaatc attgccaatg caaatatata
    11341 gataaacggc gccaaggtgc caatcgaata agtgaatttt ctgacttcaa attcccgacc
    11401 ccataacata acggtattag ttatgactcc ggcatcagca acgatatacc cttttaggtt
    11461 aataatgata aaggcaaacg tccctattcc ccagaatatg gtcggtagta tacgcaacga
    11521 attggtgaaa actgagacaa agtaataaag cagataggtt accatgacag caccaataat
    11581 cataactcta tcccagaaaa gcacaccggg atagagtttg atggacatga atacggctga
    11641 aacagtccat aagatcattg ctcccaatac aggtataaat gcacggatca aacgatcctt
    11701 ctgggcgatg cagaagaaga acatcaacaa gcagtagctg caaagtgcta caagcggtat
    11761 atatgtaaaa aacaattcaa caaatgtcat tttacacccc tgttatgttt gaaccgcgag
    11821 caattttcat gagttcaagc atctcaaaat gagaaggatt catgcgctgc ggccttttcc
    11881 caaacaactc ctggtatcgg tcaattgtgc cagtggggag gatggtaatt tgcaagggct
    11941 caattcccag caacgatttc agatccttgt aatcactgtc gatataacgg aaataaatgg
    12001 ccaaatgttc agtgataatg tcccgggtca aaccgtagtg aataccctcc ggactgactt
    12061 cgacatacag atgcataaaa gctgtgttac tttcatcaaa ttgcttcaag gcacaccaat
    12121 catggatatc cagcttggac aaggttaggg cctcatttat tgtcgcttcg gaaatgcggg
    12181 taaaaccggc aatatcgatc agattcggct ctcgatcaag ataaacaaat tggggtaaac
    12241 gcaaatcatc ctgttcgtta tacagagaaa tgcatttaaa tatatctccg acccggtatc
    12301 gggcaaaggc tcctcctttt aagttggaga tgaccagttc gtatttcatt ccttcctgca
    12361 gttcatccat caaataagtg cgcgggacat aatctgggtc ttccaggttt ttttccacct
    12421 ccggccaggg gatgaattca taaaaacaaa catcaggaaa aaaaaccagg ccatttttgc
    12481 tccaggtttc agtcgccata caggtggttt cggtgccgcc gaatatttcc agaggccgaa
    12541 tgccccagtt tgcttcgatt ttggctttat aaccggctga atccgttccg gcgcagatga
    12601 gccctttcag attccaaata tctttgggtc gaattggtac tttctcctcg agacggttgg
    12661 tccatgcctt gagcatgcgg taattcattt ttagagaatt gttcagcgga ttaatcttgt
    12721 caccggtacc gcccccggaa gcaaactgtt cgccgatttt tgcgattacg ctgctgagtc
    12781 cgaagaaaag gtccactcct tgttgcatgc ccagtttaaa tcctagtttg tttctgccgc
    12841 tgaaactcaa ggaaacagct tctttttccg atggcaacca attaaccgat atttcatcct
    12901 gcattatcaa cggggccagt cctgtaagat aaggcaacgg cgccatgcca tagagaaaat
    12961 tttccccgcc gcgcagacta aattgtcctt tttgcgtgct ggtagagagt attaaacaag
    13021 taatgaaact gcttttatgg catttgatca tactttcggt ataaggagct accttgatgg
    13081 gattttttgc tccttcccag gtggtttcga tccacagcaa aggtttggca ggaaggacag
    13141 acgctgcacg caacaataac agatcagcat aatcctcata gcgagtcaac ggtactacct
    13201 gtcgatattc atcaatactg gatggtttgg cacctttcat gatccgtctg cccagttcgc
    13261 agcgggaata catttcgatc tgttccagca tcagccggtt ctggatgtcc atatactcat
    13321 cgatggaaaa atccaaaaag ccgcaatacg tatgccagat tctctcgtat tgcttatctt
    13381 tcagaagttg atctaaattc atagccattc tccctgacgt agggtatttt ggtccattta
    13441 ttgatacgat tcagtgacgg cagtaacaat aaagtaggtg cgggccacca attcccaatc
    13501 aatatgctta aatccacttg catccagcat ttgttccatt tcctcaggtg aatatatcct
    13561 tacatcaccc ttcgtgttga aaggtgattg taaataatag ttgatcgcct gacgcagctt
    13621 ctttggccac caagggtcgg caatcaccag tcggccgccc ggttttaata cccggtacat
    13681 ttccttcaag gattgtatgg ggttgggata atggtgaaag gccgcgttgc aaacggccag
    13741 gtcaaatatg tcatcgttcc agggcaggtt atcagcatcc ccgacccgca aatcagcatt
    13801 tgttcccagc tttatttggg cttgcttgat catttgctcg gacaaatcaa ttccgcaagc
    13861 tttcatatgg ggctgttctt ttccaagtat tgccagcata actccggagc cgcagccaac
    13921 atccagcagc gactcaaaag gctgctcgct gattttttcg agaacctgcg catacatttg
    13981 ctgacagtat tgaccgtccc aggtcagctc atagttaaac gcagatttat caaaatagcg
    14041 cctgcttttt tctttatgtt tttgttgtgc caaattcatt taatcacctt ttcccagcaa
    14101 agacgccaga ctatgttgaa gactggtaag gttcggtatt ataaagggta cttgctgttg
    14161 ataaatgtgg tagtcttcca gtataatggg aaaaatccag cgatcctgaa tataaacata
    14221 aacgatatca agcagcgtcc atattatggc agcaatcaac aatagttggt tattaaaggc
    14281 caatccgctc aggcaataaa agaggaaaaa ccataatact cccggatgtc tgcaaagagc
    14341 gtacattcct gtggtaacaa cggtattccc cttttgcgtt tccaggtagg tctttttaaa
    14401 tgagactgca ccaaaaattg tataaatcat caatagcaaa accaggatgg ccaaaatcag
    14461 cgccaaccat tgccataatg tcgaaatccg tatgctgtga gggctaatga tgattgccat
    14521 ggttgaaaat gccaggatga aacttcccag tacaaccaga atattcatgt ttttgctgat
    14581 gaatctgatt ttgttaagat caaatatggc aaaacagata aaggccagac taccggcaaa
    14641 aataaaagac accattaagc acctatgtta caatattctg taaattacag catattatat
    14701 cttgttgcaa attacaagta atatcgtcaa tttcttaaat aaaaacattg tttattttgg
    14761 ctgttattaa gatacatcaa gacagactgt cgtatttgaa agaattgtac ttttttacgt
    14821 tgcaggaaca attccttgat gcagtacttc accataaatc taatatttcc tgcttttata
    14881 ttgttttaaa tgaaacattc atggataaat cggcttatat tcaacccatg atccgtagaa
    14941 tccagatttg aaaagcactc ctggattctc aatcgagcat cagcccgttg ccgaattgaa
    15001 aatattgcca tccgtttctg ctgcgcttgc cagtgcccag gcaatttctg ctttgacaat
    15061 tcctgaggca tcctgtgcaa attgtcgcag cgctgcaata gatgaggcac cgccaagttt
    15121 gccaagtgcc cagacaatca tggcttgcac gcgctcatca tcgttttccg ggtaagcgct
    15181 gatcagagcg gatacatatt gccgatcaca actgttaccc atcacccggg caacattcat
    15241 cttccagcgc caaatgtcgc gcgatgacat ataaaacata tggggttgaa tgttttgctt
    15301 aaagtattcc ttgtccatgt gcagcaggcg aggcaattca aaccagacgg atttttgaag
    15361 gactctttca tttttcggca gttcctgggc caaccacggt tcattgcgcg gacaaacgct
    15421 ttgacagcgg tcacagccgt atacccacat ccccatgggt tcgcgaagtt cacgaggggt
    15481 aagctcctcg ccgtaatagg ttagataaga gatgcagcgc tgcggatcaa ttttattggg
    15541 acccaatata gcgccggtag ggcaggaagc aatgcaagcg ttgcggcacc attggggaca
    15601 ggtaaccgca atacttggtt catcgggggc gaattccccg tcggtcaaga ttgccacagc
    15661 aaccacccat gagctttggc gggctgtccg gcttgcatag aagaaattgt tcttgccgaa
    15721 ggtacccaga ccggcacgtg ctgctgccag cctttgcgga aggtcgttgg ctactttgga
    15781 ttggataccg ttttctctta aataattgcg caattgtttg atttttatgg ccaatcgatc
    15841 cttggttatt cggtcatcgt ccagataaca acgcccgaat ttaccgacca tggaagcagg
    15901 gaaagaacct tggtagtagt tatccaaaac cacaatcatg gatttggctt ccggcatgag
    15961 caaggaagga accgtacctt cgagcaaatc caggcctttg tctgatgtcc aacggtagta
    16021 ttcggctctt tcctgcaaga gttgctgttg ggaagagaaa gggtcaattc ccgtgaagcc
    16081 gatgtcggca aagccaatct ctcttgccgc agcagcgacc gcccttttta acggcatcat
    16141 tttgcctcct ctgcgcttgt taagaacatt gtaacaagtt gatcgacata tgcatagaga
    16201 tgtcatcttg ccaagaaccc ccgtcacttt atcagtaacc gggggtttta aggagaggag
    16261 tatttaaaaa tttttatatg atatgttcca aaccttgccg ttttagtcct gttgcgaacg
    16321 gctgaggtca ttttaattga tttgcttgtt tcgattctca atacagatcg gacagacgcc
    16381 cttaaagtaa acccgacttt cgttgacttc aaaaccatcc agcccggata ttccaagctg
    16441 ggtcacttca atatgaatgt catagatttt gctgcaatgg gcacattgaa aatgagcgtg
    16501 caaagaaata tcggcatcat accgattttc gttttcctcc aggttgagca tattgattat
    16561 acctctttct acaaacaaat tcagtgtatt atacaacgtg gttttcgata gtgtaggaat
    16621 ctcgtccaac agttcgcgat agatcatatc cacggtagga tgattcttgt gttccgttaa
    16681 atacctcaaa attctaaggc gatgatgcga cggtttaatg ttgtgcattg tcaatattcg
    16741 ttccagattt tttaaatcca ctgccattgc gtctgccatc cttttgatat ggttttaaat
    16801 atgtaatcat tacagatgat tgtaattttt gcagccaagc ttgtcaagac ggattcatgc
    16861 gattactaaa ataacctgct tgctgcctgt ggtaaaatgt ctacaaggaa gcttacacag
    16921 acgattcgat tcttcaagga gtaaatttac atgaaagact ggcccgaata tatacgattt
    16981 atttttgata aactgttggc tcacggatac gaagtttttg ccgtgggtgg ctgtgtccgg
    17041 gatatgatag cgggtatcaa acctcaggac tatgatatgg tttgttcagc ctcgcttgaa
    17101 cagatcagcc aaatctttag ccgtacgctg acggttggtg ccagatatgg aaccgtgatc
    17161 gtgctgattg atcgtcaccc ggtggaactg agcacatatc ggaaagttga gttaaaccag
    17221 aaggttacgg ctttggaaat ggatctggct ggacgggact ttagcatcaa tgccatggct
    17281 atggatgtaa acggaataat atatgatccc tggggaggcc aggctgattt aaaagctggc
    17341 ttgatcaggg cgactggtga tcgggcggag gtgttgtttg ccgatgatcc gctgcgcatg
    17401 atgcgggcca ttcgcttttc ggtgacgtac gactatcaga ttgcggagtc gacgtgtgcg
    17461 gccttattaa aactgaatca tcttttggca gggattgctt gggaacggat tagggaagaa
    17521 ttgaaccgca tccttacctc tgaccggccg gcgcaaggaa tcagaaaatt gcagaccggt
    17581 ggacttatgg cttatattat tccggaattg gaagtcatgg tgggttttga ccagaggaat
    17641 ttccgccaca acaaggattt atttgaacac agtctggctg tactggaagg tgtgcctggc
    17701 caaattgatg ttcgtctggc agcgctttta catgacattg gcaaaccggc ttgttttacg
    17761 attgacgaga atggcgttgg acatttttac aatcatcatc aggccgggat ggagatgacc
    17821 cggaggatcc ttgaacggct aaaatatgac cagcagacta tcaccaatgt ctcccttttg
    17881 gtgggttcgc acatgacccg ttatgcaaag ctgagaaact cttcgttgaa acaattaatc
    17941 aagcaagtcg gagaagacaa gctggacagc ttgtatactt tgcaaagagc tgatatcatg
    18001 gcgtcagctc cgccgtttga tttttccgaa ttggacaaaa tgcagcaggc aatcgatttt
    18061 attctagata cgaaccagcc gctgtcgatc aaagatttgg ccgtcagcgg gacggatttg
    18121 ctgaccatcg gttacgctcc cggccccttt ttgggggaaa cactgcaaag acttttggag
    18181 ttcgtgttgg aagatccaag ccaaaatcag cgtgaattac tgctgcatat ggcaagcgtc
    18241 tggaaagatc aacaactata gtaaaagttt tttcgttggc ctgggccggg ccgggttact
    18301 cgtcgaaacc gggaatggta tgcaaatcga ttttgctctt cataaaatcg ccccggccga
    18361 gcgcaatcaa ctggcctttc tcattataca ggctcgattc gccgataaat aaattgctgg
    18421 agctgtgttt gactttaccc agggcggtta tgattccgct ctgcaccggt cgcagaaact
    18481 gtgtgttaaa agaaacagta taaaccatgg catcagtaac ggttgaattg acggcaaaaa
    18541 aagcagcatc atccaacaat ctgaaatagc ctgagccatg tagtgctcct gccccgtgat
    18601 gatggcgcgg gctaacccgg gtagagatct ctgcccgtcc ttcgctgatt cgaatggtgg
    18661 tttcgtcgta aagggcgaca ttatagggcg cacataaata gatatgttcc agtctcctgt
    18721 aatggtcgtt cattttggtt tgctcccttg cttaaggtgt tattggtatt atctgtagtt
    18781 taccatttat tgctgcacaa aatcagggtc aaatcttttt gtgaaaaatt cctcaacgat
    18841 cagcccgggg ctttatcgaa aaaactgttt tggttttgcg gttttcccgg tgaatacatg
    18901 ctaaaataag gaataaaaac gtaggaaaaa atgtgctgga agtatcggtc agcaacccga
    18961 taaagccaaa aggagtcagg gaggttatag gatggcagag gatagacaat ttgtagtgtt
    19021 caagttagtc catgatgatc aggtttcaga atatgccgtt ccgatctcca acgtgcagga
    19081 gatcattccc ctgccggagc cgattcgatt gccgcaggtc ccggacttta ttgaaggaat
    19141 tatcaatctg cgtggcacga tcataccgat catcgattta aaaaagagat tcgaactggg
    19201 aaattcagtg gaagtcaagg ataaacgcag cattatcatt gatttggccg gacagatcgt
    19261 cggcattatt gttgatgagg tcaatgaggt gttgcgattg tcggaagaca aaattgaagc
    19321 cccaccggct gtcatcagcg gcattgcgtc ggaatacttg attggagtgg ggaagctggc
    19381 ggacagactg ttgattattc ttgacgtgga aaaggttttt aatgaatggg aaaaagatgt
    19441 tttaaagagt gcctgctcag cttgagataa aaatcccccc ctgaaatcac ccatggtttc
    19501 agggatttct ctttcatgaa cgaggtgatg ttttgcgaat cggaattgat atcgttgata
    19561 ttgacagggt ggaagcagcg gtgaagagaa cgccgcgttt cctcagccgg gtatttacag
    19621 ttggtgaatt ggattattgc ctggccaaac ggaatcccta cccatcacta gcggttcggt
    19681 ttgcggccaa agaagctttc cgtaaaatag atcagtactt ttgccaggga atacgttttc
    19741 aggatgtgga agtcgctgtt cgggaaggtg gacggcccga actggttttg catgcccagg
    19801 cgctggcccg cacaaaagaa atcgggattg accgctggga aatgtcgctg gcgcattcac
    19861 gcaatcaggc aatcgcagcc attattgctt acggggagtg agtatatgaa attaataaca
    19921 gcggatgaaa tgaaagcgat tgatcgtcgg gccagtaagg attatctgat tcccagcatt
    19981 gttttaatgg aaaatgcagg gatgcgtacc acggaaaaaa ttgtggaact gctggacggt
    20041 gtgcaagagc ggtgtattat cattttggtg ggtcgcggca acaacggcgg tgatggtctg
    20101 gtcatcgccc gccatttgct caatgctggt gcccaggtgg atgtatttat gtgtgctgaa
    20161 gccgatcagt tgagtgggga tgccgccatc aattatgcta ttttggaccg gatgaagggc
    20221 aggattcatc ctttgcgcca agaagcagac cgggaacgct tcatcatggc tttgctgcag
    20281 gctgacatgg tcgtcgatgc gttgtacggt atcggtttta gaggcagttt gagcgagttt
    20341 gaagcagaaa ttgccagact ggtcaatcaa agtcaaagca tggttctggc agttgatatt
    20401 ccctccggtc tggaagccga taccgggcag gtgaacggga tcgcctttaa ggctgactac
    20461 acggtgacgt ttgccttgcc caaacgaggg atgatcctgg gcagagatcg gcaacatgta
    20521 gggcagctaa cggtggccga tatatccatc ccgggtgcct tgctcaagga cgaagcactg
    20581 caactgcaga ttattacaaa agagatggtc agcccatatt tcaagccccg aaattccaat
    20641 acccacaaag gcagttacgg ccatacgctg gtcattggtg gttccatggg aatgagcgga
    20701 gcagtcgtca tgactgcctg ggcagcactg cgctgcggtg caggtttggc aaccttggcc
    20761 ttgccggaca aaattgtaag tgccgccggg ctggtacctg aaattatgag caggggattg
    20821 ccttccacgt cgcaaggagc cattgccgcg gaggcttgtc ctatccttga gaatctcttg
    20881 gggactgttt cggtatgtgc gctgggaccg ggaatgtccc gttatggaga gggacatgcg
    20941 gttgtaaaga cagtgttgga aaaagccggt attccagtgc taatcgatgc ggatggtcta
    21001 aatgccctgc aaggagacac ggagatttta agagatcgac aggtacccat cgtcattact
    21061 ccgcatccag gtgaaatggc gcgtttgacc gggagatcgg tagaagaaat tcagaaccaa
    21121 cggatcgata ttagccggga ttatgcaagc gaatggggag ttacgattgt tctcaaggga
    21181 catcatacta ttgtggcaca tccctccgga caggttttta taaatgtgaa cgggaatcct
    21241 ggcatggcaa ctgccggcag cggagatgtc ttaagcggga tcattgccgg cctcatggcc
    21301 cagggaatga aaccgactga agccgctgta gccggtgttt acttgcacgg ctgggcaggt
    21361 gatgaagccg cccggcatca gggacaaaga tctttaacgg ccactgatct gattcaccgg
    21421 atgagtgatg tgatcaattc cttggaaaac aacggataga gcatacagtg tttgcaatat
    21481 gaataatggg aggtaggata aatgctggcc aaagacatta tgaccagaga tgtgattacc
    21541 gtcagagcag aagaagaggt ggaaaaagtc gcccagcttt tgttggacaa caagatcagc
    21601 ggggtgccgg tggttgatga aaattaccac ctggtgggga tggttacgga aaaagacctg
    21661 atggtcaaag ccacggaact aagagtacct ttttatataa cattatttga cagtattatt
    21721 tttctggaaa accccatacg ttttaaaaac gatctcaaaa aatacactgc ctcacaagta
    21781 aaggatgcca tgaccaagaa ggtatactgg gttgaggaag ataccccggt tgccgaggtg
    21841 gcggcattga tgcaaaaaga aaacatcaat cgagtgccgg ttctgagaca taaaaaagtg
    21901 atgggaatca taacccgtaa tgatttacta aaaaccttgg tgaaaaagta atggataatc
    21961 gcagcacaag ctgggcagaa atagatttag gagcgctcag aagaaatatt tccgccatta
    22021 gggcgttgtt gcaaccggat acgaggttga tggcagtggt taaagccaat gcctacgggc
    22081 acggagtgct gcaagtggct cgggtctgta tggagaacgg tgcggattat ctcgcagttg
    22141 caaacgtaca ggaagccatg gaacttcgtc aggccaatat ccatgcgccg attttggtgt
    22201 tgggcatggt acccgaagaa caggctgcct tgatgagccg ccacgatatc gaggcggcgg
    22261 ttgctgatct ggaaagtgcc caaggctatt cgcgccaggc cagtaccgtt cctccgcttc
    22321 gattgcatgt caaactggat accggcatgg gtagaattgg atttcaaacc gatgaaaaaa
    22381 cagtcggtga aattaaaacc atccagcaac tgcctaatgt aaccttgcag ggtgttttta
    22441 gccattttgc tgcagctgat gccgccgaca aggagtacag ccgtcggcaa acaagcgact
    22501 ttgaggcttt tatcgagtgc ttgcaagcca acaacattca tatccctgtc aaacatttgg
    22561 ccaacagtgc agcgattatt gatttgcctg aaacccaata caatatggtg cgcagcggga
    22621 ttattatgta cggactttat ccttctgacc aggtcaagcg agaaaaactt cccctggaac
    22681 ccgtgatgcg cttaaagacc cggattgctt tcctaaaaac cctgtcgccc ggcagatcgg
    22741 tatcctattg ccgaaccttt gttgctcccc aggatacact ggtggcgacg gtacccatcg
    22801 gttatgcaga cggatacagc cggcttttgt ccaatcgggc ctgggcggtg gtacgggggc
    22861 aaaaagtacc tttgatcggc aatgtttgca tggatcaatg catgtttgat gtgaccggcg
    22921 tcgagggtgt ggaaaaaggc gatgaaatga ttctttttgg ccgcccggaa gatggcgtta
    22981 ccgctgatga tctggctcac atcaccggca cgatcaatta cgagattgtc tgtacgctag
    23041 ggcagcgtat cccccgtgtc tatgtgggcc agggaggata aacaattatt ggatttgcgg
    23101 gaaaaagtgc atgtctatat aataaacgta tgcaatatga catttactat atgggggtgt
    23161 acttcttgcc agactccaaa agaatctcta tcaccatacc tgtagacctc cttggagaac
    23221 tcgatacttt atgccacctt gagcataaaa atcgcagtga aatcgtcagg caagcaatcg
    23281 ttgactactt ggaggaacgc agaaagaatg caatgatcga acaaatgaag aaggggtatc
    23341 tggagatggc agcaattaac cttggcattg ccggtgaaga caaacaaaac tgaaaatgca
    23401 gggtttaaag caaaaagtgc tggagtgatt aacattatga ttaaaagagg tgaaatctac
    23461 tttgcccagc taaacccggt aatcgggtct gagcagggtg gattaagacc ggtgctggtg
    23521 gtccaaaatg atattggcaa tcagtatagc cctacgacaa tcgtgctggc cattacctcg
    23581 cagataaaca aagccaagct gcccacccat gtcgaattaa aagcggaaac ctatggtctg
    23641 gaaagagatt ccgtggtgct caccgagcaa atcaggacca ttgacaaaaa acgcctgaaa
    23701 cagcgggtgg ccgtgttgaa tgatgagacc atggagaaag ttgaccaggc gctgggcatc
    23761 agtattgaat tagccaatcg aattaataca taaagcagca aatactgcaa gtgattccaa
    23821 gcaggttctt ctaggacctg ctttgcttat gcaagcacgt aaggacttgg agacgaatat
    23881 gatagacaaa atggccaaac ctgactttgg actgaaaaaa gctgtgcatc aatcaggggg
    23941 aatgaatttt gcgtccagtg attggaatca cttgtaatta tgtgcagact gccaatttgt
    24001 taagtttgcg tgatgcttat gcggaatcca tttatatggc cggagggctg ccgctgattc
    24061 tgccggtagc cagtcaacaa gatctgtgcc ggcagtattt tgatctatgc gacgggtttt
    24121 tgttttcagg cgggggagat gccgatccgt attggtttgg acagactgcc gacagagatt
    24181 tgggagaagt aagtcccctg cgggatgctt ttgaaataca attggctcgt atggcgctag
    24241 ctaccggcaa accagcgttg ggaatctgcc gggggtgtca gatcctaagt attgctgacg
    24301 ggggcacgat cttacaagat ataaaaagca agcaactgca cgatcaaaaa gctccccggg
    24361 actatccaat tcatgatata ttgatcgaga gcggcagttt gttggcaagt atcgtacagt
    24421 ctgactgcat ccgggtcaac agtttccatc accaggccat cgacaaggca ggggataacc
    24481 tgatcataag cgcctgggcc cgggacggta ttgctgaagc ggtggaagga cgaaacggat
    24541 tcttttacct gggggtgcaa tggcatccgg aatgcctgca ggatatctgt tcgcagcgtt
    24601 tgtttgcggc tttgacagag caatccaaat acttttaaac tgaggataat ggaagacagt
    24661 cattcgaacc gcggctgtca attgatcata gaatgtagaa caggggaggc aatgaacaag
    24721 atgtatgcga ttcgaggcgg aaaaatttta accatggctc aaccagggac tgtcgatgat
    24781 gccatgattc tgatcgaggg cgagcggatc agacagatta attacaacct gcagccgccg
    24841 gatggttata aagtgattga ggcacagggc aagtatattt gtccgggctt catcgattca
    24901 catacccatc tgggtttgga agaagagatt tatcgcatcg aaggcgatga tgtcaatgaa
    24961 atgacccatc cggtgacccc tcagttgcag gcagttgacg ggatcaacta ctttgatctg
    25021 ggttttgagg atgcgctgcg cggcggcatc accagggcta tggtcatgcc gggcagtgcc
    25081 aatatactgg gagggcaggc cgctttcctg aaaacactgg cttccagacc tgcagaaatg
    25141 atttacaaga acccttgggg attgaaagcg gcgctgggtg agaatcccaa gcgagtctat
    25201 ggggctgaca agaaatctcc gcaaacccgc atggccagcg ccagtctgtt gcgagaggct
    25261 tttttcaaag ccgcccgggt gattgacaag ttggatcttg agggaggcga agcttatgcc
    25321 caagagccta tcttcaaggt gctgcgtcgg gagatgccgc tgctgctgca tgttcaccgg
    25381 gcggatgaca ttctcacagc actgcggatc atggacgaat ttcactttga tcttctcatt
    25441 caacatggga cagaagccca tttggtggca gacgaactgt gcgagcgcaa ggtaggtgtt
    25501 tttctcggtc cgctcctggt caatcgggcc aaggttgaaa tgaagtcagt tgcttttaaa
    25561 aatgcccgcc tgctggcaga aagaggcgta aaattttcat tgatcacaga tcacccggtg
    25621 gttccgatcg aacatctcag ggtgtgtgcc gccttggccg tacgggaagg attgagtgaa
    25681 gacacagcgc tgcaagccct cacggttgag cctgcttctt tcttgggggt ggaggaggaa
    25741 ctgggcagta taaaagttgg gaaaagagct gatctggtta tcttcaacgg acatccgctt
    25801 gatttccgct cggttgtgga ggaagttttc gtagatggtc tgctttggag gaatcgtatt
    25861 gaatagagga gatttattta ctgtatacat ggagggggct atgatgaccg tttgtgtaat
    25921 tgcttcctat caggaagaat acagtggaga ggaaatggtg atcctggcgg ttatcaaccc
    25981 ggataacctg gtgcatgttt ccagggaaga gctggattac ctttttcccc gcagcaagtt
    26041 attaaactga tatcagtggt ctgcaaaatc tctatcgatc aattgcaagc atatgtcaac
    26101 cacttcggga tcatacagaa taccccgatt gtcagtgatt tccttgatgg cttcctccag
    26161 accacgcgtc gggcgataag gtcgatgggc agacatggct tccaccacat cggcaacggt
    26221 aataatgcgt gcctccagca tgatctcctg accgcgcaat ccattgggat aaccggaacc
    26281 atcgattttt tcatgatgct gcaggacgat atcagcaacc ggccagggaa attcaatttc
    26341 tttcaggatc tcgtaaccga cctgggagtg actctttacg attgccaatt ccggttcaga
    26401 gagtattccc ggtttactca gaatttcgat cggtacggtt attttgccaa tatcatgaag
    26461 acttgctgct gcatacaagc ccgccagctg ttcatccgac aggcccatct cccttgcaat
    26521 agccaccgcc atggcagcta cgttgctttg gtgcccggct gtataagggt cccttttttc
    26581 gattaccgca cccagggcat tggctgcctg tctcattagt acccgcattt tttccacata
    26641 caattgcgtt ttgcgcaacg tctggctgtg cgactggcga gtgcgcaggg tgataatggc
    26701 ataatccagg ttgtctgcca tttctttgag cagattttgt tcctcgccat taaatatgcc
    26761 ggtttcatca gcataaatac acaaagcgcc aaaaggtttg ttttgattca agagggggat
    26821 gctcatggat gatttaaagc cttgctgaag agcactcttc agccatggcc ggtccttttc
    26881 gttttttata tcccggacaa ttacgggttt cccagtgatg atcgacatgc cggtaggacc
    26941 tgctcgccgt cgcttgtcag tcaaactaat tttaaggttt tccacataat tttctgcttt
    27001 gcccgcccag gctacgggaa ggacggtttg ctttgcatct tcctgcagat acccaaccca
    27061 gcacatgctg tagtcgccta tgtctacaat cgtctggcag atatcatgca gcagctcttt
    27121 ttcagtctgg gcatggagca tgatttgatt gcatgcgctg atcgccttca aagaacggtc
    27181 aatccggttg aagtcggtga ggtcgataaa agttgctacg gaagttttag tgccggggat
    27241 caattccacg gccatcaatc cttcgcgagg ttggttttgt ttattgatca ttctggtttt
    27301 gtatttaaat ggtgctgcat ttggtaatgc ccggcgtaat tgatgaaatt gtttcatctg
    27361 ttcaacgaag tccgggtgga taatttgcgg ccacttcata ctggtgagtt cctcgttggt
    27421 gtaaccgata atccgttcac attcgcggtt gacccgggaa atggacatat cttcttccaa
    27481 aagcagagtt gcagtcccgg tggtatcgaa aatcgtctgg tagtagttct cactggcttt
    27541 gactttggct tctgcctggc gctgttctgt gatattttgc atcgaggcca gagtgtattt
    27601 actctcaggt atgagtgaaa catttaataa taaatattga acgttgtcat tcttatcaat
    27661 aatcacgatt tcgtatttgg agggcacaga gcaaggatcc aacatgcgtt gcgcatgata
    27721 tttcgttagc aactgtttgt tttccggaag tttaatgata tcaaggatat ttttgccgat
    27781 cagttcttga cgggaatacc ccaccagtat ttcatactgc aaattcacaa caatgatgtc
    27841 aagattttcg tcagccacca ggatgggcac attcagattt tcaaaaatac cttgaaaatc
    27901 attgaaaaaa gttaaatccg tcgccgatgt tttcgggtca tttgattttg gtttcatatt
    27961 cggcaggatc atttcctcac ctcccacgac caccggaaaa aaatagaact atgataagct
    28021 tatcataaca tttttcccct tgctttctaa tccctgctga aagtaaattc tcaattttct
    28081 gacatgagtt ctaaattgcc aacattttca tcaagggttt tctgacgggg ttaagaattt
    28141 aaacaataca gatttagtta ttggagttga agtgatgaag cggttttatc ttatttgttg
    28201 tttggctatg gcattttggc tggcaggtgc tgtgccgttg tcagctggca ttggtccgat
    28261 cataaaaata aatggacaga caagaaccta tgagcctcct tgccaaattg ttgcgggacg
    28321 aaccatgatt ccgatccgtt atgtgattga agatcctgct ttgcgaggcg cggtggattg
    28381 gaatgagaca aaagccgagg tcagcgtctg gtgccgggac aagcattttg ttttccggat
    28441 tggcagccat accgccagcg tcgataatca aaacctggtt ctggatgtag ccccatacat
    28501 atatcaaaat cgtacctata ttcccctgcg ttttctggcg gaacaactgt ctgcccgagt
    28561 ggagtggaac gcttctgccc gggaagtgag cgtccatttc gctacgcaaa aagaccgggt
    28621 gtttgcctac tattattacc actcctttga tgaattcaag cacaatgccg aatcgataac
    28681 ggatgtagcc ctgcgctggt ttgaaaccaa tgcggaggga gatcttttct atgaatacca
    28741 ggatgattat gagcaaattt tgcagtttgc ccgggagaaa agcatcaaaa cccatgccag
    28801 cgtagtcttt atggacaagg atggcttgca tgcactgctt tccgattctg tcaaacggaa
    28861 atatttgatt gcccagttgc ttgaagaggt taaacgaaac aactacgacg gggtaaacat
    28921 tgattttgaa ttcctggggc aggctgatcg cgactctttt gtgcagttct tgcgggatct
    28981 taaacagggt ttgggcagag acaaagagtt gtcggtggcc ctttttgcct gcaccaagcc
    29041 gcagagttgg ctggcgggtt acgattatgc cgctatcggc gggattgctg accaggttgt
    29101 cgtgatggcc tatgattacc attacaaaac cagtccggcc ggaccggttg cccccctgtg
    29161 gtgggtggaa gacgtggttt cctatctaaa aaccattatt ccggcggaaa aattattgct
    29221 gggactgcct acctatggtt atgactgggg tgaggggatc gacggaacca ccatcactgc
    29281 tgccaaattg tatagcctgc agggcaaata caagttgacc gaatcattcg ataaagccag
    29341 tatgagtcct agctacagtt atattgacaa caacggtgtg cgtcatcaga tctggctgga
    29401 aaacaaggcg agtttgcagg ccaagctgca agttgcccag gaatacaagc tggcaggggt
    29461 ttctttttgg cggatcggca acgggtttac ggaattgtat gaactgctgg aggaatggca
    29521 gtaaggtggt taaccggctt attgccaggc cagggcaata atgcaggaaa ctgaaccggc
    29581 ctaacccaag gggatgattt cccctttgaa atgggtattt accaaatcca gcagagctgt
    29641 gagggcgggt gacatccact tgtttttatg acagacaatt tgggtataca tttcgatttt
    29701 tggctggcgt aatcgcaagt cgaccatgtt gccggcagcc agttcttttt ctacggcagc
    29761 atagggcagc aacgcaattc ccaggccgct tagaacaaat tgcttgatcg cttcgatgct
    29821 gtcaaactcg taggaagaca gaggctggat tttagattcg gccaaatgct tttcgatgac
    29881 cgaacgataa ctgcaatcac gttcggtaaa gatgaatgat tctgtgccga tatcagccag
    29941 tgcaaaatct ccttgggcag cccgcggatg ttcacgattg gccacaatga tcatcggttc
    30001 ttcacacagg atgcgtacca gcaacgcttc atcccggata atactgtcca gggaaaaggc
    30061 tacatcaatg gtgttgtcac acagccagcg ttgaaaatcc agcggggttc cctgacggat
    30121 gaccagtttg acctgaggat aaagccggct gtattccttg agcaaggccg gcagtttaaa
    30181 aatgcacagg gattcggcga tgccgatgtt taatatgccg ttgggcgcag ggcttgaggt
    30241 taatgtgtcc ttgatttcat cagcgtattg tagcagtttt ttagcgtgca aaagcaaagt
    30301 ctcgccatcg gccgttaggg caattcgctt gcccagacgt tcaaacagct tggtgttgaa
    30361 ctcctcttcc agcaaacgga cctgggtcgt caggttggac tgggcatatc ccatcttttg
    30421 tgcggcgcgg gtaaaactac cttcttcagc caccataacg aatattttca actggcgtaa
    30481 ctccatgccg cccccctata gttatcaata ataatgattg atattatcaa atcaatctat
    30541 tttactggat aataaataga tgatacggta aatttgttaa aaggaccagc acatttttat
    30601 ttgtttcttc tttgaggatt actgctgttt gcatgagagg aagggtgtcc atgggaaaac
    30661 cgcttttgtt tgccggatct gctgtagcca tcattacccc ttttaaagat gagctgattg
    30721 attatgagaa actggcagag ctgattgagt ttcaaattga aaacaattca gatgccatcg
    30781 tgatttgcgg gacaaccgga gaagccagta ccatccctga tcaagaccac attgccgcga
    30841 ttcagttcac ggtcgagagg gtgaaaaaaa gattgccggt tattgcgggt acgggcagca
    30901 accataccag tcacggcatg gatttgtccc gggcagcaga ggcggccggg gcagatgcca
    30961 tcctgtccgt aaccccttat tacaacaaga ccacgcaaaa cggtttgtat gcccatttca
    31021 aggccattgc cgacagcatt cgcattcccg ttatccttta caatgtaccg accaggacca
    31081 atctgaatct gaatccggag acggtgcgcg agctttcctt gatcgacaat attgttgcca
    31141 taaaagaatg caatatggct caggccggcg atgtcataaa cctgtgcggg gaagatttta
    31201 ccctttattc cggcgacgac aacatggtgg tgccaatgct ttctctgggc ggaaaagggg
    31261 tcatttcaac aaccgccaac ctgattccgg ccgattttca tcagatggtg agtctgtatc
    31321 tgcaaggtga tgtggaaggg gccagaaaga tccaactaca gacgttaaac ttgatcaagg
    31381 ctttgtttat tgaggtaagc cctattccca ttaaagcagc tttaaatatg ttgggcatga
    31441 aagtaggcaa ctgtcgcatg ccgctggtgg aaatgagcga agccaatcgg gcgatcctgg
    31501 aacaggaaat gaaaagttat ggattgctga aataaccaat gctttggtaa aaatattttg
    31561 accgggaatt ggatgatccc tgttgcccca aaatgtttcg gactcttatc aatgcctaac
    31621 gcattgcgga gtccgactgt ttttctggat tcgtcaaaat caagcaaatc atatataatg
    31681 ttgatggata tatcgcgtct gacgttcggt gatggggtca gcacacgatt aaaacagcag
    31741 gagaggggaa aaaatgatga ttgaacagca aggactgttt ggagaggaag aaccggctga
    31801 aaagcaggca gggaaccctg ttgtccaaga atctctagcg gatattccct tcctgccggg
    31861 gattaagttc gaggaagcct atccggatgt aaaagatttg gacatgcttg gaaagaaagc
    31921 cttgggatgc cggggatgcc gcctgcggga gacctgccag caagtagtct tcagcgccgg
    31981 cagtgccgat gccaaaatca tctttatcgg ggaagggccc ggcagcgatg aagatgagca
    32041 gggtgtaccc tttgtaggaa gggccggtca gttgttaaat cagatcttgg cggcggctga
    32101 attgaaaaga gaagaagttt atataaccaa tgtcgttaag tgcagaccac ccggcaatcg
    32161 tttgccaaat ccagatgaag tcaaggtgtg ccgcagttat cttgaggctg agattagaat
    32221 catcaagccg cacatcattg tctgcctggg agcgatggcc acccagacag tcgttgatcc
    32281 caaagcccga atcggtcagt tgcggggaag atggctgcag aggaatggta tccggataat
    32341 gccaacgtac catccggccg ccttgctgag aaatcccagc tacaaacgtc cggtttggga
    32401 agatttcaag ctgattcggg atgtctacaa gacgttaaca taaagggaac catctttgag
    32461 gtgaagacat ggaactaatt atcaacaatg aagaagaaat gctggtgctg ggcaagaaga
    32521 ttgccgcgca gctggaaccc gatgatgtaa tctacctcac cggcgaactc ggggtaggca
    32581 agactaccct ggtgagaggc attgctcgtg cccggggata taatgggcgg gtaaccagcc
    32641 ccacctttac cctgatgaat atctatgcaa ccgatccgcc gctttaccat tttgattttt
    32701 atcgtttgga atcgggcgat actgtcgatc tgggactgga agattatctt gagaaacaag
    32761 gcatatcgct gattgaatgg cctaatctga cggatagctc gctgcctgat gaagcgttgc
    32821 taattaccat aacgcttttg tatgatgatt acgaactggc tcgtaaagtg gaaatcgcag
    32881 cccggggggg caaatacgaa aaaaaaataa aaggattgat ggcaagtgta catcctggcc
    32941 atagatagcg ctaccccggt agctggtctg gcgctgctga atgatgaaaa ggttatccgc
    33001 gaggatttta ttaattttaa aaaaacccac tccgagacgt tgatgcctgg ggttgatcga
    33061 atcatgcatg attgtgagct gacgctggat caactgggag caattgcagt gacaatcggc
    33121 cccggctctt ttaccggctt gcggattggc ctggctgccg ccaagggatt aagcatgtcc
    33181 agcgggaaac ctttaatcgg catttcaact ctggatgtac tggccggcaa tcttgttttc
    33241 agtgacaatc tggtttgtcc gttgctcaat gctcgcaaac aagaagtgta tacggctttt
    33301 tatgaagccg gtggcgcata tccccggcga ttaacgcaag acatggcctg ttccccgcag
    33361 gaagcagctg cacgggcaag ggaactggct catcgccttg gcaaaccaaa catcaccctg
    33421 ttgggggatg gcttttatcc atatgaagaa tactatcggg aatacttcag ggaagatttg
    33481 cagatcgttc ccggaccgct gatgctgccc cgggctgcag tcttgggaag cctggcctgg
    33541 caaagggcca ggcaaaatga ttttgatgat accttcgccc tgcgcccggt ttatgtaaga
    33601 ttgtcggaag cagagtacag gttgggcaaa ggagcgttat agttgctgaa taccagatac
    33661 atgattcgaa aaatggctga aaacgatctg gacaaggtga tgattattga acaggaatca
    33721 ttcagcctgc cctggtccag ggaatcctat ctgggggaac tgaaaaatga ttttgccaat
    33781 tacctggttt gcgatgatgg tggagaaata gctgcctacg gaggtgtctg ggtggttttt
    33841 gaggaagccc atatcaccaa tgtggccgtg gccattcggt accgcggaca gggggtgggc
    33901 agtgatttaa tgaaggaact ggaaaaaatc gcccgctcca aacatgctac tcgcatcctg
    33961 ctggaagtca ggcccagcaa ccaggttgct ctggctatgt atgacaagct gggctttatc
    34021 caaaccgggg taagaaaagc atactattcg gataatggag aagacgcact gataatgacc
    34081 aagtatttat tctaatgaag ttggtggcac acatgaaaga aacattgatc cttggaattg
    34141 aaagctcgtg cgatgaaacg gctgcagcca tcgtaaaaaa tggccgggaa gtcctggcca
    34201 gcgtgatcaa ttcacagatc agtattcatc agcaatttgg cggtgtggtt cccgaggtgg
    34261 cctcccgcaa gcacattgaa aatatcgctt ccgtggtgga aactgctttt ctggaagccg
    34321 gcctcagaca tgacgaaatt gacgcagtgg ccgtaaccaa tcggcctggc ttgattggcg
    34381 ccttgctggt gggcttgtct tttgccaagg cgtttgccta tggcttgggc aaacctttga
    34441 ttgctgttga ccatctgcag ggacatatct gggctaattt tctcgaacat cctgatatta
    34501 gctttcccgc catttgtctg gtcgtttccg gcgggcacac cagtctgctg cttatggagg
    34561 gacccggaca aagccgcctg ctgggcgaga cccgtgatga cgctgccggc gaagcgtttg
    34621 acaaagtggc acgttttctg gggcttggct acccgggcgg tccggccata caaaaagcgg
    34681 cgcagcaggg aacagcaggg atttataacc tgcccagggt cttcctggac cgggaggatt
    34741 atgaatttag cttcagcggc ttaaagaccg ccgccatgaa tctctggata aaacaatcaa
    34801 gaaaagggga agcccgggtt gaagatatgg ccgcggagtt tcaggcggcg cttgtcgaag
    34861 tcttggtgga aaagacgata cgtgctgcaa aagaatataa agccccctct attctcatgg
    34921 cgggaggggt tgcagccaat cagttgcttc gacaattaat gacaataagg gcgcagcaag
    34981 aaggtcggca attgttttat ccatcgcttg gcttatgtac tgacaatgcc gccatgattg
    35041 ctggaaaagc ctatttagac tatgttgcag ataattttgc tcccctccat gtgaatgcac
    35101 acccgggcca aaattccttg tgatttcgcc tgtggataat gtgcataagt ctgttgataa
    35161 ctgttatttc ttgatttcta agcttttgca aaacggattg aaaaaccgag gggcgataaa
    35221 ttggtaagca aacttgtgga taatttccga agagaggatt gatgcgggtg catccggtgt
    35281 tactagaatt tgggagaata aagatatatt cctggggatt tatgttagcg atagcagtaa
    35341 ttgtttcgat cattggcctg accaaaatgt tcaaacgtga aggctatgat ccggattcag
    35401 tgattgattc ggttattatc ctggtactgg gggggatact gggctcacgt ctattatata
    35461 tatttgctta tcaatggcag gaactgctga ccgatccagc agcagtgctg tctttgaaag
    35521 atggaatcca aggtctggta tggtatggag cggtaatatt ggatgtcccg ttgtttattt
    35581 ggtatctgcg tcgtaaaaaa tatcctttat ggaacattct ggacatgttt gctccttttg
    35641 tcgcgttggg ctatgccatc gtcagggttg gatgtttcct gaatggctgt tgctacggtg
    35701 aagttaccag ctcggtttgt ggggtggtct ttccttatgt ggacagtttt acccggtatc
    35761 cgacccagtt gttcagtact tttatgaact tgcttttatt cggcttgctg ttgtggtatt
    35821 atccccgccg caagtatgat ggagaagtct ttgtattcta ttttatgggc tattcaatct
    35881 atcgtttcct gatcgagttc atgcgcgtaa gtcaaatcaa tttcgggccg tttaccatga
    35941 gtcaaatcat tagtatggcg attttcataa tagcattgct gctccatatc tataagatta
    36001 aacaggtcgc ggcacaaaaa gaataaatca tgtgaggata atcaaatgca tatcaatctg
    36061 caaggttcca aagctgcgtt gcgcagtgaa atgaaagccc aacgcggctt gctcgatatg
    36121 aaacaaatcg agcaagccag tctggctatt gccaggcgtc tcaacgactt gctgccggtg
    36181 caaaaagcca aaaccatcat gggatatgct gcaatgaaaa aggaagtcaa tctggatgcc
    36241 ttttatgaac aacagctgca gttgggaaaa acgatctttc tgccgcgggt agagggagat
    36301 caaatcaagc ccgtcaagtg gaacgggtgg aaggatatca aggttagttc ttttggggtt
    36361 cgcgaaccgc tgggggaaga atttccagca gccgagatag atgtggtgct ggtaccgggg
    36421 ctggcttttg atccaggtgg tttccgcgtc ggatacgggc ggggctatta tgatcgtttc
    36481 ctgccggggt tgcgttctga tgcatttaaa tgcggtatct gttacgaatt tcaagtggtg
    36541 gattcaactt ttccccatga ccgggatgtt cccgtacact ggattgtaac tgaaaagtcg
    36601 gaactggttg tcaattggga ttttttctaa aatcggcaga cataaaaatg ccgggcaggt
    36661 tgatctttcg cttgcccggc atcattattt gttggaaatt cttattgctg ttcttccggt
    36721 ttatcgagca ccacttcttt taaggctgca aatacgccgc ccactccggc tagatcagcc
    36781 gggataatta atttggtggc ttgcccttcg cccaattgtt tgatggcttc aagcgacttt
    36841 atagccagta ctttgtcgtc tgcaccggca tccttgatca tggtcaggct gtccgcaaag
    36901 gctctctgaa catttaaaat cgcctgggcc tcaccttctg cttccagaat tttcgcctga
    36961 cgagatcctt cggcggtacg aacagcagct tcttttactg cttccgctct caaaatagct
    37021 gattgctttt cgccctcggc ctctaaaatg gtggctgttt tttgtccctc cgccagcaag
    37081 atggcttggc gtttctctcg ttcagcccgc atttgttttt ccatggcagt ctgaatgtct
    37141 tgaggaggca ggatgttttt taattccacc cggttgactt tgatgcccca tttatctgta
    37201 gcctcgtcta aaatggtgcg cagcttggtg ttgaccagat ctcttgaggt cagagtttga
    37261 tccagttcca gatcacccac gatgttacgc agggtggttg ccgagagatt gtcgatggcc
    37321 atgatcggat tggcgatttc gtaggtataa cggaatggat ccgttacctg ataatagacg
    37381 atcgtatcaa tcatcatggt tacattatct ttggtgatga ccggttgggg ggcaaaacta
    37441 accacctgtt cacgcatgtc gacaatagca cggaaacggt caataaaggg aatgatcaca
    37501 ttaatcccct gttccgccgt tttgtgatat ttccccagcc tttcaacgat gcctacggta
    37561 gcctggcgaa tgattttgat ggcggaaaca gcgatgatga tcacaaacaa aaccaaaata
    37621 aaattcgtta tataatccat atcaataccc ctttctaatg tttgcttcag atttcaataa
    37681 attcctactc ttgctcggtc ggccggacaa tcaatttgac gccgtcgata tcggtcactt
    37741 caattctgcg gtcggcttcg atctcactgt ccgagatggc cgtccagatt tccccgttta
    37801 cttttacctg tccgtactgc agcggcctca gcgcagtagt gctgatgccg tgttgtccga
    37861 ccaaagcctt gacattactg acggtatctt tggttttaaa gagttttacc gccagggggc
    37921 gagtaaaaat gataaaaacc aaggtaaaga atgcaaacaa cagcaattgc cattgtaatg
    37981 ttgtcagcca ttccaaacga acgccaatgg ccaccagcaa agctgctaaa gccagccaga
    38041 ggaaccacaa acccatggtg aaaatttcaa tggatccgca taccacagca acaataaccc
    38101 attgagcagt tgtcatgatt ttcacctcct cagtagacta taatgtagta cgaataaaag
    38161 atttcgcagc tatcataacc atcttaccat aatctatacc attaatcgca tcttgcttat
    38221 agattccaat attatatgca ttgttgcgcc agtctaaatt ggtatataat gaggcatgaa
    38281 acaattggag cctccctttg gttgatgggc ggttcgaatg tgttcaaagc ttatggcgga
    38341 ctactgttta gtttggagga atgaataatg gaaaccaaag aaatcattga aaaaatcaaa
    38401 gaactggctc ctcagggaaa aatcagctgt gctgatgcac gacagctggc agagaaaaca
    38461 aatattgaat acgggcagat cgggaaactc tgtgatgagg ctggggttaa gatttacgga
    38521 tgcgctttgg gctgttttta atttttatca taaaatgaac gtgcccaaca cttatttatg
    38581 gtggggggag gaataatatg caggacggat atggaagaga aatcaattat ttgcgtattt
    38641 ccgttacaga tcgctgcaat ctgcgctgtc agtattgcat gccggaaacc gggatcaacc
    38701 atgttggaca tgaggacatt ctttcgttag aagaaatttc caggatcgtc agggtggctg
    38761 cttccatcgg catccgcaag gtcaggttga ctggaggaga gccattggtt cgcagaaacc
    38821 tcagccaact ggttcactat attcatgaga tcaaagagat tgatgacatt gcgcttacta
    38881 ccaatggcgt tttgtttgcc gccctggggg acgagctgaa aacagccggc ttgaatcgaa
    38941 ttaactttag tttggattct ttggtggccg aaaagtttcg gtttataacc cggtcaggga
    39001 atctgccgat tgttcttcaa ggcattgaaa aggctctgga cttgggatta catcccataa
    39061 aaattaatac cgtactcatt aaaggtttca atgatgatga aattatggat tttgtagcgc
    39121 tggcgttaaa atatcccctg catgtcagat tcattgagtt tatgcccatc ggcgacctgt
    39181 tgttttggcg tccggatcga cttatgacca gtcatgaaat cagacagcgg atagagaaag
    39241 agtatgtttt ggaagaaacc tacaaagttc ctggtagtgg gccggccagc tactataaaa
    39301 tacaaggcgg attgggttcg atagggttta tttctcccat gagcaaccat ttttgtgcgc
    39361 gctgcaatcg tctcagaatg acggctgatg gaaagctgcg ggcgtgtttg tatgaacagg
    39421 gggaaaccga tttgcgcgat gccctcaaac gtagttcaga cgatgaagaa ctcagacaat
    39481 tatttctggc gacactccgc tccaaacccc agcggcacca tatgcagagc ggttgggggg
    39541 atgacaatcg ccgcaagatg tatcagattg gaggataatc ggatgggctt aagccatatt
    39601 aatgaaaaag ggtatgcccg catggttgat gtcagtgaca agcaggatac ggtccgcgtg
    39661 gcacgggcgc aggcgattgt atgtatgcaa ccggaaacct tggccatgat ccgggagggg
    39721 accattaaaa aaggtgacgt tctgtcagtt gctcaagtcg caggaattat gggggccaag
    39781 cagactgcac agatcatccc catgtgtcat cctatttttc taagcggggt tgatcttgat
    39841 ttttgcatgg atgaagtccg gtgcagcata atcattactt cgcaggcgaa aacaacaggg
    39901 aaaaccgggg tggaaatgga agccctcaca gctgttaccg tggcagcctt gacgatttat
    39961 gacatgtgca aagcattgga tcgctggatg gaaatcaacg acatcaggtt gctggaaaaa
    40021 tcgggcggta aaagcggaca cttgatcaat agcaaagctg aataagtctt gggtgagggg
    40081 gaagagcatt gatacagggt cgtttatatt cgatttgtat ctcgtctgag cgcggtcagt
    40141 tgaagaatga agttgacgaa gcacagctgg ttcaaggtta tggcatcgaa aatgacagtc
    40201 atgccggtga ctggggccgc caggtaacct gcctggcaaa ggaaagcgtt gagaaagtaa
    40261 accaggaaaa aaatctgcaa atcggtccgg gtcagtttgc cgaaaattta ttaatcgaag
    40321 gtttggattt tccctcggtg ggggtgggcg gaaagctcaa aatcggggcg gaggccatac
    40381 tggaagtcag ccagatcggc aaagaggatc atcctagcat cgtgaccgaa acttttggag
    40441 tttcgctgct gccatacgaa ggtttgtttt gccgggtgat tcaaggcggt cctataaaaa
    40501 aaggcgatga ggttgaggtg ctgcaaaatg tataattgcg ggatactggt tttgagtgac
    40561 aaagggtcac aaggagaacg ggaagacctg agcggcatgg agatcaagaa aacaatgtcg
    40621 ggaaaggatt acgtttttac ctattatgag atcattccgg atgacgaaga aatgatcagg
    40681 gaaaaactga tcctggcctg cgatgattta aagcttgatt tgctgcttac ttcgggtggt
    40741 acaggttttt ccaaacggga tgtgaccccc gaggctacct tgagcgtgat tgaaaaacga
    40801 gtccccggaa tccctgaagc cattcgttat catggtctgc aaaatacccc caaggctatg
    40861 ctgtcacgtg cagtggcagg tatcagaggc aataccctga ttattaatct gccgggcagt
    40921 gtgaaaggcg tacgcgaatc tctggaagca attctgcctg ccttggatca tgggttggat
    40981 attttgatcg gatcggcggc agaatgcgga cagggctaac ggcaaaaaac gctgcggcgg
    41041 atgaataaaa agcccgcggc ggatacatac taatgttgat atcaggtagg tttgattgag
    41101 ttttttacat ttgtgtactt gacatatcct gcctattatt attaaataat attagcactc
    41161 gcaattaacg agtgctaaca acaaatttat gaggaggtat accccgtgaa gattcaacca
    41221 ttgggagatc gcgtggtcgt caaagttgct gaagtaaaag aagaaaaaac caaaagtggg
    41281 ctgtatgtgc ctgacacagc aaaagaaaag ccccaggaag cggaagtaat ggctgtaggt
    41341 cccggcgcat tgaatgataa gggcgaaaga gtatcaattg atcttgccgt gggcgacaaa
    41401 atcatcttct ccaaatacgg cggcatggaa atcaaagtgg atggagaaga ctatcttatt
    41461 ctttccgcgg aaagagacgt actggcaaaa ttggtgtaat tacgttttaa atatgaaact
    41521 aaggaggcta cataaatgat atccaaggaa attaaatttg gcgaggaagc cagaagagcg
    41581 cttgagaaag gcgtagatac gctggccaat acggttaaag taacgctggg accgaagggc
    41641 agaaacgtag ttttggacag aaaattcggt tcccccacca ttaccaatga cggtgtaacc
    41701 attgcccgtg acattgatct ggatgatcct tgggaaaacc tgggctgcca actggtgaaa
    41761 gaagttgcca tcaagaccaa tgatgttgct ggtgacggaa ccaccaccgc taccgttctt
    41821 gcccaggcca tgatcaaaga aggcctgaaa aacgttgccg caggtgccaa tcccatgatc
    41881 atgagaagag gcatcgaaaa agccgttcag gctgccatta aagaaattca tgctatcagc
    41941 caacctgttg aaaccaagga atcgattgcc caggtagcat ccatttctgc cagcgatccc
    42001 gatatcggca gtctgattgc cgatgccatg gaaaaagttg gccgtgatgg agttattaca
    42061 gtagaagaat cacaaaccat gggaacccaa ctggaagttg tagaaggcat gagctttgac
    42121 cgcggctata tttcacctta tatggtaacc gattcagaga aaatgcaggc ggttttggat
    42181 gatccctttg ttctgctgac ggacaagaaa atttccgcaa tcagtgaaat cctgccggta
    42241 ctcgaaaagg ttgttcagac cggtaaaccg ttactgatta tcgctgaaga aattgaaggc
    42301 gaagccttgg cgacactggt tgtcaataaa ttaagaggca cctttacttg tgtagctgtt
    42361 aaagctccgg catttggaga aagaagaaaa gccatgctgg aagatatcgc catcctgacc
    42421 aacggcagag ttgtatccga agaaaccggc agcaaaatgg aaaatgttga tctgaccatg
    42481 ctgggcaagg ctgccaagat cgttgtcaag aaagaagaaa ccatcattgt tgacggagct
    42541 ggttccgaag cggatgtcaa aggccgcatc ggtatcatca aaaaacagct ggaagagacc
    42601 gaatccgaat atgataaaga aaaactgcag gaaagaatgg ccaaattatc tggcggcgta
    42661 gctgtcattc aggtgggtgc agcaaccgag acggaattaa aagaaaagaa acaccgcatc
    42721 gaagacgcgc tggcggcaac caaagctgcc gttgaagaag ggattgtatc cgggggcggg
    42781 acggctctga tcaatgccat ggttgccatt gatgacgtgc tggcggaagc cgatgaactg
    42841 accggcgtaa aactggtcaa aaaagccttg gaagaaccgc ttcgtcagat tgccaacaat
    42901 gccggtgtgg aaggatctgt cgtagttgag aaggtaaagg cagcagaaca tggcattggc
    42961 tttaatgcac tgaccaatgt ttatgaaaac atgattgccg ccggaatcat cgacccggcc
    43021 aaagttaccc gttctgccat tcagaatgct gccagtattg cagctatggt gctgactaca
    43081 gaagcaattg tttctgagaa acccaatccg aacgaaaatg ctgccatggg cggcggaatg
    43141 ggcggaatgg gcggaatggg cggcatgatg taagataacg cccagttgct taacatttag
    43201 aacgcaaagt tactgtttgc ttaaacacaa aaccaaagac ctcccgattg cttctgcaac
    43261 cgggaggtct ttgcattaca gcagatttaa aaaagccagg agcattgact cctggctttt
    43321 ttaaaaacta taaatcgtag tacaattcaa attccatagg atgaggaatt tcatttactt
    43381 tgcgggcttc tttgcgtttg ttctcgatcc agatatcgat caaacgttgg ggaaaaacat
    43441 ctcctgccag caggaagtca tgatcttttt ccaatgcatc cagtgcttcg tccaatgatt
    43501 tgggcaacga tttgatcttg gcctgttctt ccttgggcag attgtacagg ttgacatcgt
    43561 aggggccaaa accttcggca gtcgggtcga ttttttcctt gattccgtcc agccctgcca
    43621 tcaggatggc ggaaaatgcc agataggggt tgcaggtcgc gtccgaggaa cggaattcaa
    43681 agcgtttttg ataaggactc ttggcatagg ccgggaccct gatgaccgca ctgcgattgg
    43741 ccgtcgcaaa acaaagattt accggagctt catagccggg aacaagtctt ttgtaagaat
    43801 tggtgctggg attggtaaat gccaataccg ccggggcatg ttttaagagt ccgccgataa
    43861 agtacagcgc ggtcttgctt aattgggaat agccgttggc atcatagaat aatggttcac
    43921 cgtctttaaa aagatgcatg tgtacatgca taccgttgcc cgcttccgcg aagagaggtt
    43981 tgggcataaa ggtgacggtt ttgccttcct ggaaggccag gtttttgatc aggtatttgg
    44041 ccagcatggt cttgtcagcc atttcccgca tgccggcaaa ttcaacctcg atttcaatct
    44101 gtccgggacc gcccacttca tgatggtgat atttgaccgg gatattgttt tcttccatca
    44161 gaacacacat gcgtgacctt aaatcataaa ggatatcctg aggaggagca atgtgataac
    44221 cacctttgtg aggtattttg taaccctgat tctggaattc attgtcaccg ctgttccaga
    44281 tggcttgctc agcatcaatg gtaaaaccgg tatggttggg actgcattca tagcttacgt
    44341 gatcaaaaac atgaaattca tattccggtc cgattcgcat ttcatcagcg ataccggttg
    44401 atttcatata ttcttcagct ttgaaagcaa tcgaacgggg atcctggtca aagcgacgat
    44461 tttccggttg ggtaataata aaaacatcgc cgatcatgct gagggtagga acatcggtga
    44521 aaggctcatg gacagctgtg ctcacatcag ggataaatac catatcactg ttttctactg
    44581 gtgcaaaccc ataattggaa ccatcaaagc caatgccgta aactagtgta tcaggtttga
    44641 accgggccgt cggaatactc agatgacgcc atctacccat gaggtcgatc atcttaaaat
    44701 cgatcatctg aatagaattt tctgcgcagt actccattac ttcgttgtaa ctggtaaaca
    44761 cacgcattgc ctccttaatt atttaattac ggtgcaaatt atatcagaag ccgggataca
    44821 tagtaaagaa tataatatac ggaatattgt atacttcatg tttttggaat aatcttctgt
    44881 ttttctcaag ttatataatg gattgcactt taataaaaat gtaataactt tttcatgaag
    44941 gaaaacagat ttgaccaata catttggtaa gttgcattgg tgatgtgggg ggagagtgag
    45001 gaggattaaa aaaacctatt ggttgtcggg gattgctgtt atattgctgc ttgctctggt
    45061 tgccagtgcc ggccagatta tggattatta cgaaaaatca aaactgcaac ccgatgttga
    45121 gcttcagtcg gctttaaaaa aaatgccgca gcttaacagt ttccgataca gactgcagtc
    45181 tggattcacg gttgataacc gggaggaggt gataagcagg gtcgaaggcg agacaacaga
    45241 tggcaatacg catataaagg gggagatggt caacacaccc attgatatct attatattga
    45301 ccgcaccatt tacaactggg attccttttc ccaaaagtgg ctggtcattg aaagcggcac
    45361 cagcaactcc ggggaattac taatatcaga gctgaatccc ttgtccaatt ttcaatataa
    45421 aaacgtgcag gacataaaaa aaattggatt taccaaagta gatggtgagg aatgtctggt
    45481 agtgggatgc aaaccgtcgt tacaaaatca actgctggaa agtttgtgga aaaatttcga
    45541 ataccggtta tggatcgatt tccataatcg ggtaatcaaa aaggcagtgc tgacagcggt
    45601 gaaccaacgc aatgaacaaa cccgcctcaa aattgaagct gagttttttg atatcaacaa
    45661 aaaaattacg atcaaaccac cggaacgtac tgcccgggat aaaaaataaa atagaaatga
    45721 gctaaaaaga ccgccccgct gtctgaaaaa acaggcagcg gggtttatca ttgcgcgtat
    45781 ctgttttgca cccgatggtc aggtgagaat ggagtataaa ccttacgtag ttgtggaaaa
    45841 ttacctattg atgtgcgatt ttgatattgc cggttggcag gaaacaacgt aaagtgatta
    45901 caaggtttga caatgcgagg aggtaaaaca tgatatttgc atttgaaccc ctggagggcg
    45961 gttattctat accgatgcgg gtggttggag ccaatatccc ctatgaatgg ctgatttata
    46021 ttttgatgtt tatcccgatt ggctttttca tttatggctt ttatcagcgg atcaaagtat
    46081 ggaaactggc tcaaggtgag gaacatcgca atgaccagct gggcagacgc tttttttcct
    46141 ggttggtcaa cagcatgggg caactgcagc tggtgagaaa accgctggcg ggctggatgc
    46201 acttcatgct cttctggggc tttatgtttc ttttgcttgc caccgccaca tttgcttcct
    46261 ggtcaaagat tggttttccc gacatgactg gttttttata tacattgatt tcgtggctgg
    46321 ctgatgtcgg cggtttcttt gctctgttgg ggattatcgt gttgtttggg atcagatatt
    46381 ggcgcaaacc ggaccgtctc aatgataccc gatcctcgga cggctggatg ctgctgctga
    46441 tttttctgat cctgctgggc ggatatgttc tggaaggcat gtggctggca gcgcaaatgc
    46501 agctatccac caccatcgcc cagttggatt acgaacgcgt ggcttctccg gttggctgga
    46561 tgtttgcggg tttatttcaa ggaatgacct tggacaatgt gctgctttgg catcgaatca
    46621 attggtggtt gcatatggtg ctggcctttc tgtttattgg tctggttccg tataccaaac
    46681 tctggcacat ttttaccggg atgatcggtt attttagccg cgactttaaa cccagtgcca
    46741 atcgcatggt ctatcatctt gaagaggctg aacacttcgg ggtggaaaac atagaggaat
    46801 ttacctggaa agacttgttg gatctggacg cctgtatccg ctgtggtcgt tgccaggaaa
    46861 attgtccagc ttattatacc ggcaaaaccc ttaatcctaa aattaccctt attcaggcca
    46921 tgaaaaaaca tcttgacgac aaagtgccgc aattaatgct taaagagccg gttcacgatg
    46981 agttggccat gacttcggca gcagaagcaa aagtcgtcga tgcggccgag gccagtctga
    47041 tttatgatgt ggtcacgccg gaggttctat gggcttgcac caattgccgg gcctgtatgg
    47101 agcactgtcc gatgtttatt gaacatttaa acaagattac agacatgcgc cgcaatctgg
    47161 tcatgtggca gggagacatg ccgggagagg cccagagcgc ttttaccaat atggagagaa
    47221 actacaatcc ctggggggta ggctgggctt cgcgggctga ctggatgggg gaacgtggag
    47281 tgcgggacaa aatcaatctc ttgccggaag acggccgtga ttttgaatat ctgctttttg
    47341 ccggttgtgc cgtggctttc gatgatcgtt acaaacgcgt aggcgaagcg ctggtcaaac
    47401 tgctggacaa ggccggcgta agctttggct atttgggaac ggaggagtgg tgctgcggtg
    47461 attcagcccg ccgtttgggc aatgagtatt tatatcagtc cctggcagcc cacaatattg
    47521 aaaccttcaa ccaatatggg gtgaagaaaa ttatctgcat ttgtccgcat ggctacaatt
    47581 gtttaaaaaa tgagtatccg cagatggacg gggtctatga agtctatcat gcgacagaat
    47641 tcctggccgg gctgattgtt gccggcaagc tcaagccgca gcgcatgaac aatatacgaa
    47701 tgagttatca cgattcctgt tttctgggca gacacaacgg gatttacgat gagccgcgca
    47761 agattttgca ggcggtgggg gcaaatgtac aggaaatcga taaatcacgt cgctttggat
    47821 tctgctgtgg ggccggaggg ggacgcatgt ggctggagga agaggctgtc gctggcttca
    47881 aacgtatcaa tcaaagtcgt accgagcaac tcctgcagac ggcaccgcag accattgcca
    47941 gcaactgtcc gttctgttta accatgatct ccgatggggt caaggaagcc gggcaggagg
    48001 aacaggtaaa ggtgctggat ctgtgcgaaa tcctctggaa cagtgtggaa taatcctcat
    48061 ttttcttcca ccagttcgat tctctcgccg ttcgggccgt gcaaaaagat aattctttta
    48121 tggctggaca gcgttttagg ggcaacgttt tcaaacactg cccctaattt gcttaaccgt
    48181 tcaattgtat ctgccaggtc atcgacatga aaagccaaat gattgattac tcctggggat
    48241 gtgcatgact gggagggatt ggtatattcc agcaactcaa tcaacaagct gtcgcattgc
    48301 agattgacag ctttgatttc atcgttctgc cagcgactga caggaataca acccaaagct
    48361 tcgctataaa agcgctctga ttggtcgcaa tcgctgacaa cgatgcccag atgatcgagt
    48421 ttcatgatct gcacacctcc ggaatatcag tggtttacca ggcttccggg aaagccatgt
    48481 tggtatagat gtcttccagt tgaaccttgt gacctttttc catgttggcc agattcatga
    48541 acaatttttt ctgcgccgca tcagcactgc tgttggccag gtccgtgtac attttcatgg
    48601 catcttcttc gtttttcatg gccagggcga ttgcatccac cggtttcatc tgcattgaaa
    48661 gtttgggttt atcaactgtt tctgaaactt tgtagtccgg aagagctcca aagttcaagg
    48721 gacgcacatt gctattgaga taggatgaca acaaatcccg gtgacctaat tcctgttcag
    48781 ccaactcctt gaatatcgat ttgatattcg gatcttcaac tttttgagat accccgagat
    48841 aaaactcata ggcttcgact tcattttcaa tggccatgga aagtatgctt atataatctt
    48901 ttgaattccc catttgtttt ccgctcctcc ttgctaaaat tttctttaag tttaaagcat
    48961 tcaaacgttt ttggcaatca atgcattatt ataaaatcgt atccaaatag tattggtatt
    49021 atgcaaggtt atgttttttc ttgcggttag tggttagaat atggttgtag gagttaacaa
    49081 atcgttgaac aggaggaata aatttggctg aacaatctac cagaaaaaga aatgagatcg
    49141 atgaaaaata taaatgggat ctggcaaaaa tttatgtgaa tgagacgttg tgggaagagg
    49201 actttcaaac cttacagcaa aaaatcaagg cgattgctgc cctgcagggg accatcccca
    49261 attcggccga agcattgctg aaagtgttgc agacttcaga cgagatggga agaattactg
    49321 aaaaactgta tgtttacgct cgtatgcgcc gagatgaaaa caatgccgat caaaactatc
    49381 aggcgctttt cgaccgtgca gagcgattga gtgttgaagc cagcagtgcg gctgcctata
    49441 ttgtgcctga aatattggcg attcctgagg cagacctaaa aaactatctg cagcaaaaca
    49501 aaggcttggc tgtatatcga catgccctgg acgagatcac ccgccagaag gagcatatcc
    49561 tttctgtgcc ggaagaacgt ttgctggcca tggcggcaga tctctccacg gcatcctcgc
    49621 atatatttac catgctcaac aatgcggacc tcaagtttcc ggtaatcaag gacgaggaag
    49681 ggcatgaagt ggaactaacc aaaggtcgat acggcagttt tatggagagc ggcaaccgcc
    49741 aggtgcggga agatgctttt aagggcatgt acagtacgta tcgcaagttt atgaatacct
    49801 tgggggccag tttaaacgcc agcatcaaaa aggatatttt ttatgcccgg actcgcaaat
    49861 atccgtctgc tcttacggca gccctggatg gcgacaatgt gggcgtagac gtctatgatc
    49921 gtctgattga agctgtccat aataatctgg aaccaatgta tcgctatatg aaaatcagga
    49981 aacaacagtt gggcgtagat gaactacata tgtatgatat ttatgtcccg ctggcgaaag
    50041 attacaagat gaccattccc tatgaagaag cctgtgagaa aatgctccaa tccctgcaac
    50101 cgctggggca ggaatatctt ggccaggttc aggcaggttt gcaggaaggc tggatcgatg
    50161 tttacgaaaa cgagggcaag accagcggag cttattcctg gggcacttat gatacccagc
    50221 cttatatact gatgaactat gacaacaaac tggatgactt gtttaccctg gcgcatgaaa
    50281 tgggccattc catgcattca tattactcca atcataatca gccttacatt tatgcccagt
    50341 attcgatatt tgttgccgaa gttgcctcga ccgtcaatga atcgctgttg atcgactacc
    50401 tgctgcaaca cagccatgac aaacaagaaa agatctatct gctgaatcat tacctggaac
    50461 aatttcgagg gactgtatac cgtcaaacca tgtttgcgga atttgaaaag attattcatg
    50521 ccaaggctga acagggggaa gccattacgc ctgaattgat gaatacagtt taccgggatt
    50581 tgaataaact gtattacggc ccggaagtcg tcctggatga agaaatcaat atcgaatggg
    50641 ctcgtattcc tcatttttat tccgcattct atgtatacaa atatgccacc gggttttccg
    50701 ctgctactgc catcaaggaa gccatccgcc gtgaaggaga agtcgcggta gaaaagtact
    50761 tgggattttt aaaatcgggt ggctcggatt atccgattat tttgctgcaa aaggccggag
    50821 tcgacctcac tacgcctgaa ccagtcgata gtgcattaaa atattttgcc cggctggtag
    50881 atgaatttga aagcttgttg taagaatatg tattgataaa tccgggcaag gtaatcgaaa
    50941 acgtttgcat agtttttaag gaggggtatc gggattgacg aacagcaaac gggaaagcat
    51001 tttcgatgag caaattgatc tgatcttgcc gcagttggag agctataaca attttgatct
    51061 actgatcggg attcctttct acgggaacaa ttgtggtttg catcaggtac tgcaatccgt
    51121 ggaccaggtt ctgcagaatt ggatcggcag acggcagttg attgtctgta taggcgatgc
    51181 caatgcagca gagatgatgc ataccattga aaatcttcaa ttgtatcatc cgtatctggc
    51241 ctttatcctg ccgcctgagg ccagcgggcg gggcatgagc atccgtgccc tgctgcaaat
    51301 cgcccggaaa atggaagtcg atctgctggt ctttgatgcc cctatgggaa ccagtcatgg
    51361 tccgggtatc aacatcgatt ggctggaagg cttgcttacg cctattcagg gacaatatga
    51421 cctggttgtc agcagccgga agaggcatgg cggcataacc agcattgcct ccctgctggc
    51481 catgccgttc ctggaaacct tctatggcag tcgtctgagc gagcctctgg gcggaatata
    51541 tgccatttct catgattttg tagaggaact ggtgacagaa gccaagttct ggacggatac
    51601 catagccggt tccggcatcg acttctggct gattacccgt gcgttgtgct ggaataagca
    51661 aatctgcgaa ttgtatttga acgagaatcc tcaatcttgt agcctggaac gcaacagtgc
    51721 agtgtttata gatgttttaa aaacgatttt tgcctgtctg gggcgtgacc atgctctttg
    51781 gctgccggag cggctggtca ccaaagtagt cgatatcctg aactataaaa aaatgcccgt
    51841 ggttgagtcg gccggagtcg attgggatga actgcgccag gaattaaaaa agcattacca
    51901 aagatccgag gatacgatca ggttaatttt gccggaagag aacgagcaac agttgcgtca
    51961 gttgatgaca gcagcaaccg gagattatca cctaagcaat tctgcctggg tgagcaacat
    52021 ctacaaaatc ttcttgtatt acacgtttag cgaagaagtc agtcagaagg atttgttgca
    52081 tgccgctgcg gcattgttca atgcgaaaat tgccagttgt gtcctggaat tgaatcagtt
    52141 cggtgccggt ctcaaggccg cggatgaatt cgaaaaagat gaactggtca gtcacaaact
    52201 ggaattgacc cgccagcaat tactggtgga attccacaag caaaagccgc tgctcagcaa
    52261 acaatggctg ggaagatctg aacaaaccgc accgccgctg gtcccctttg gttatatgga
    52321 atacatcccg ggtcgtccca ttgttgtgcc taaaagaatc agcggcaagg ataaacatgt
    52381 cgtcaatacg gatgaactgt ttaaaaaact gcgcaaacta tacgaagacc ggtttaacca
    52441 tttcctggcg gaaggactcg ggcttccagc ccacgcagaa cctgatcgga tcattgccgc
    52501 aatggaaaag tttatggctg ctgccgagcg cacggtagat gaaatgtttc ccgggaattt
    52561 gaacgatcct gacgggatgg agcagtttgt gaccgggctt tttgctatgg tcccacatca
    52621 gcagatgttc accatcaata cggatctttt aaaggagatg ctgatcagat ttccgccggt
    52681 caatctgatg atcccgctgg gattctacag cccggcggag ttgcttgcaa acatcgatcc
    52741 ccgggatgcg gtgacgtatg ccaatctggt tgacagctgg agttacaatg accgtgactt
    52801 ggtgtggctg accgagcaac tgaaaccgga aagctttggt tgggcacaga tcaaaccatt
    52861 gatcgtcaac aagtccttgc cggaagggtt gtggtcgcat accaagattt ccaatctgaa
    52921 tcgcttaacg gctcgtatca ccatcaaagc catggaaccg ggcaaaggag ggaaatatcc
    52981 caagctgcgt tatttcactt ctctcattcg gcggatttgt ttggcagagc attattcgct
    53041 gttgtttcaa cagaacgtca aggaacgcaa aaacgtgggc ggcaaggccc gtaattcgat
    53101 gaccgccatg gaaaagggtg atgacttctc cgcccagcgg atctttgaaa acagcaacca
    53161 ccgggccctg gttgaaaaaa ttggtgccat ggctttggct ttgcagcagc ggggcatgaa
    53221 cgaacctgcc cggatttttc aaatgatggc agacggctat gggctaagcc aggttctgga
    53281 ggatggagca tttttaactt gtaccgtgtg gagttgggcc agttacaagg ggggcttaaa
    53341 cctaccaacc ccgatgacga ccagtattga aaaccgttgg ttcaaccatg aatttctgga
    53401 acttttgtac aaggaactgg gctacgacaa ggaagagatt ttgcaaacag catttcgcta
    53461 tctacagtcc ggcaagagcg gacatagtct gctggatgct ctgctcccag cccgacccaa
    53521 ggatattcag gtggtggtgc aggaaactac taacgaaccc agtaaaacac tggttcgcta
    53581 tgagggtaat cccttgctgg agcccatcaa ggaacatgac tgggagtgca aatacgtcct
    53641 caatccgggc gcgattcgca tcaaagacaa agtctacctt ttctatcgtg ccgtcggcga
    53701 agacaatgtt tcccatatcg gtctggccat aacggatggc tacagggtat tggaaagatt
    53761 ggacacaccc atcttttcac cggctacgcc cgaggaaaaa atgggctgcg aggacccgcg
    53821 cctgatccag atcggagaac gtatctggat gctctatact gcgtatgacg ggaatattgc
    53881 ccagatcgcc gcggcctcga tcagtttgca ggattttcta gcgcgcagat ttacctcatg
    53941 gaaacgggaa ggtctggcat ttaaaaacat ctgggataag gatgcgatcc tcttccctga
    54001 aaaaatcaag gatagataca ttctctacca tcgcatcgag ccgagcatgt ggattacgta
    54061 tatgaaagat ctggtctttc ccagtcgtga aaaacatgct atcataattg gtccccggcc
    54121 gggacggatg tgggattcat taaagattgg tgccggcgcc cagccgatta agacccgcta
    54181 cggatggttg ctgatctatc acggtgtgga tcataattac gtatatcgtt tgggcgttat
    54241 cctggtggac ctggagaatc cgcagcgcgt tatttaccgc tctcccaacc ctgttctgga
    54301 gccgattgaa gactatgaac tgggtctgtc gggtgcctgg gtgccgaatg tggtctttac
    54361 ctgcggagcg gtggctggta cagagaaaga agtcctggaa gacgaggatg agattctggt
    54421 ttattacggt gcggcagata ccagtatcgg catggccaag gctaccctgg cagaaatgat
    54481 tccggaaaaa tttcgctgcc gctaaataga gcttcacgcc gaagactttt tcgtgctaag
    54541 ataagcagca aaagagcgaa gagacgaggc gattccagtg aaatctttgg tactggctga
    54601 aaagcccagc gttgcccggg aaatcgcccg ggtcctcaag tgcgtaaaca aacaaaaagg
    54661 ctatatggaa ggcagtcaat atgtggtaac ctgggcgctg ggtcatctgg tgaccttggc
    54721 ggaaccggat gattatgacg ccaaatataa acaatggcgg ttggaagatc tgcccatgct
    54781 gccggaaaaa atgaaattaa aggtgatcaa accgacagcc catcagtatc aggtggtaaa
    54841 aagtctgatg aaccgggcgg atataaatga gcttgtaatt gccacggatg caggacggga
    54901 aggagaactg gtcgcccggt ggattatgga acaggcgcgc tggaaaaagc ctttcaagcg
    54961 cctatggatt tcttcccaga ccgatgccgc gatccagcaa ggatttcgcc aactaaagcc
    55021 aggccgggag tatgacaatc tttatgttgc cgcggtatgc agggctgaag cggattggct
    55081 gattggcctt aatgtgaccc gggccctgtc ctgcaaatac aatgcccagc tgaccgccgg
    55141 acgggtgcaa acccctaccc tggccatgat tgtgaacaga gaaaatgaga tccaagattt
    55201 tcagtccgtt gactattgga ccatccgggc agattttggt gattattatg gagattggcg
    55261 cgatcgccac aaccaggggc gtatttttga agccggtcgg gccgagtcca tcgtagccaa
    55321 agtcaaggga caaaccggga cgataaagga catgaaaaaa gaaaccaaag cagaaccgcc
    55381 tccgttggcc tatgacttga ctgaactgca gagggacggc aatcgccgct tgggttggtc
    55441 tgcccagaag accctttcca tcctgcagag tttgtatgaa gtgcacaaag tggtgaccta
    55501 tccccgcacc gacagccgtt atattacgcg agatatcgtg cccaccctgc cggcacgttt
    55561 gcaggcaatg cagaccggac cctatgtcga ttttatcaga cccttgctca gccaaaaaat
    55621 tcagcccggc aaacgatttg tggatgacag caaagtcacc gaccaccacg ccattattcc
    55681 caccgagcag aagctcaatt taaacgcttt aagtgtggat gaaaagaaac tctatgatat
    55741 cattgccaga cgtttcctgg cggtgctcta tccgtcctat aaatatgaac agacgaccat
    55801 taccacggtg gttgctgaag aaatcttcct gtctcgcggc aaggtggtta aggaagccgg
    55861 ttggcgcgcg ctgcaggcgc agatatcgga aaaagaagag aataaagagg aacacctgcc
    55921 ggagcagctg ctgcaggaaa ggcatcaggg agaaacccgg aaagttggga attgccgttt
    55981 aaacaaggcc aagaccaagc cgccggcgcg ctataccgaa gccaccttgc tgacggctat
    56041 ggaaagcccc ggaaaatata tagaagatga agaactgcgc gaagccatga aaggcagtgg
    56101 tttgggaaca ccggcgaccc gggcggaaat tattgagaaa ctgtttaata ccttctatat
    56161 cgaaagacag ggaaaggaaa ttctgccaac cgccaaaggc agccaactga tcggcttggc
    56221 accgccctcc ctgcgttcac ctgagctgac ggcccaatgg gaacagcaac tgacagacat
    56281 tgcccgcggc aagagcagca aaggtcaaat gatcacggcc atccgggagg atgcgcggca
    56341 gatggtaagt gccgtagctc aatccagcga cgaatacaaa cccaccaatg tcagcaaaag
    56401 caaatgtccc gcatgtggaa agaacatgtt gctggtcaac acccggcagg gaaaaatgct
    56461 ggtctgccca gaccgcacct gcggtcatcg ccagccggaa aaacaggcgg atacagacgt
    56521 tttcagccga aaaaagcagg gagaacgggt taatcccagg ctgttggccc gttacagtga
    56581 tcaaaaagac cttggtcaaa gtctgggtga caaactcaag gaagctctgc agaataaaga
    56641 aaaaaaacaa taaaaaagcg aacaagataa aataatttgt acccagggag tcgttattca
    56701 tcggctcctt gtttttattg cagattttac acgcaatatc acggaataca attgcttcca
    56761 aaggcaggaa agacggcgga atttgaagaa acaaataata actgagaaag agcggatatg
    56821 gaatccaagg aaataaatta caatcagtca aacatgctgc aaaattgagg atgttcgctt
    56881 gtttttgcgc atgatgcagg ctgttttgtg ccttatttgg tgacaaccca attggtgcaa
    56941 tctgttgaca agggacagaa tgcggatcat tgactgttgg ttcgatgtag ggagaacaga
    57001 gggggtactg aggaatgctg attaccaata cagaaaccca tattatgaaa caattacgcg
    57061 ggataaaagt gcatgacaag tgctgcttta tttatgaaac agaagaagaa tgggaattct
    57121 atatgatccc ttttctcaaa cagggtctga tgaatggaga aaagtgtctt tatcatgttc
    57181 agcgcaggga cgaggactac ctgttgcgtt gttttgccaa gcatgggatt gaggttgaag
    57241 catgcttggc atccggtcaa ttggagattt taaaaccgtc cttcaccttg cctatactcc
    57301 aaaccagcaa tatggatgaa atcatccaaa catttaagtc ggaattagac caatattgcg
    57361 cgcaaggctt taaaaacctt cggcttacca gtgaatcgat ttccaaactt atggatgccg
    57421 aagaagcgac aagtcgtttt ctggagttgc agctaaggct aaaccgggat gttttcccat
    57481 attatgcgat aagatcaata acccagtttc atcgccatga agacagcccg atggttttaa
    57541 aagacgcgat catttctcat ccgtgtatat tcattggtgg tcaaatttat cgaaatcctg
    57601 ccacgatttc tccggagatt tatttccagc acaaggataa agcctgggaa gcggaatact
    57661 ggctgcgtac ccaggaagcg ctgattgatt cagaaaaaaa atatcgcttg atatttgaac
    57721 attccaagga tatgactaca atcattgatg ccaaaaccta tgatattgtc ttgattagcc
    57781 cggtgcattc caaactcctt ggctatactc ctgaggaaat gattgggaaa aactgcctgg
    57841 aatttatcca tcccgaccag cgggaaacga tggtgaaaga attcaaatca tcccatactg
    57901 ccggcaaggg aatttatgca atacgcaagc aggacggctc ttatctctgg acggaaggaa
    57961 aaggcgatat cattcataat gtcaacggcc atgaccaaat tattttgttc acacgcgata
    58021 ttcatgataa gaagctggcc gaagatgctt tgcatagaag tgaacagaat tgccgctatc
    58081 aattgcgata tctaaataca ttaataaata ccatgaatga attgtgtctg acctatgata
    58141 gcgatagttg tcttaccttt gtcaaccagc ggttggtgga aaagcttggg tacagtgccg
    58201 aggagatgtt gggcaaacct ttgctggatt atgttgtgga tgatcacaaa caagccgtgg
    58261 cagagcaatt gcagctgcgt ttgcaggggg aatttggcag ccatgaacac aagtttatct
    58321 gcaaagatgg cagtgaattg ctggtggaat tgaaaggttc accaatttat gaagacgagg
    58381 aaattaccgg cggactggtg ctggccgatg atattacttc gcagcggcag atggaaatgg
    58441 atatggcccg tctcggtcaa atgcatctgg taggagaaat ggctgtcagc atcggtcatg
    58501 aaattcgcaa ccccatgact acggtacagg gtttcctgca gatcttgagt caaaatcatg
    58561 agtttaacga acaccgtgag tatttcgact tgatgctgga tgaacttaaa cgggccaacc
    58621 tgatcatcag tgaattcatt tccctggcca agaacaaggt ggccaattta caatcccata
    58681 atctgaattc catcattcag gcactggcac cattgctgat ggcggatgcg ctgaacggtg
    58741 acaagaatat caaatttgaa ttaagcaagc tgcccaatat tttgctggat gaaaacgaaa
    58801 ttcgtcaact gattcttaat ctggtccgca acggcttgga agcgatgcag aatggcggca
    58861 atgtaacggt taaaacctgg caggaaaaaa acaaagttat gttatccgtc atggacgagg
    58921 gcgatggaat aaaggaagag attcttgaaa aattgggaac ccccttcgtc agcaccaaag
    58981 aaaacggcac tggtctggga ttggcggttt gctacagtat tatctcccgg catcaggcca
    59041 tactgcaggt taaatccagt cagaccggaa gcagttttac ggtagcattt aactgtgttt
    59101 aatcttgggg gagaagcttt caacagttgg caattggcta tagtgattgt gcctgaggca
    59161 gatcgtcgct gcaatatccg gtcagcagca ggtcgcttgc cagcaaagat aaagctgctt
    59221 cggctccttg attaaggttt acaccttgac gggtgagggc atcaaaacag ccgccgcttt
    59281 caacatcata caagggcagg ccgttgagat tggcgccgcg ataccattga taggccaacc
    59341 gcagcaaggt tttatattca tttcgaccgg tgacctgata ggcttcatga caggcaaaaa
    59401 caatcgaggc agcgtccacc ggctgctgat cccacaacgg catcttgcct ccccggacat
    59461 accagccttc attgccgacg atatttaaat agcctttggc aaacaaatga tgattgagaa
    59521 agccgagtga tgcatgggcg attttcagat atttgcggtt gccgatgctg gcataaaccg
    59581 caaacagggc atggggcatg atgccgttgc agtaggtgat tttgtcctca aaccaaagcc
    59641 aatcgggact gtgttcatta tgataaagag ccagcagacg atctgccagc atatgaataa
    59701 gattgttgat ggatttggca ttgataaagg gaaattgccc tttgcaaaga ccgatcagac
    59761 aataagccag gccacgggca gagtttagaa aaggcaagca ggggactgcc cgttccagca
    59821 tgttggtaca gcttcctgcg atcagcggcc actttgaacg gctgcctgca ctgcaagccc
    59881 aaaaagcccg tccaaaactg tcttcagaat tcaagtctga caagtaatcg ccgtgcagca
    59941 acaggttgca ccaggtattg ttcgcctgcc agctgtgctc gagataatgg gtataagtca
    60001 gagccagagg atagccttca tcccccatgg ctaaagccac cagcaaggcc cgagcattgt
    60061 catccagcgt ataaccactg tccggatcag gatgatgaca tttggagaat tgcaggatcc
    60121 ccgttgtggt ggtcatgtta tacaagtgtt tatagtttgg caagaatctt ttcctccccc
    60181 agtcggttca aatgcagaac ctgttcaaag aaaacaaggt attctctccc aacgtttgac
    60241 caggtccagg ttttgcccag ttcccaagcc ttttcctgca ggcttttctg cagttcctgc
    60301 gattccagta tttttttcag cccttgcgcc agtaaatgcg gttcggcatt gggcacaagc
    60361 agaccgcgtc cgccttttag aacttccttg gcataggcat aggcagttga aacgatggcc
    60421 cttccgcacc ctatcgcaaa agccatggtg ccgctcaccg cctggtcaag attgggatag
    60481 ggactgaggt agatatcggt catgtaaaga tatttcccca attcttcgtc ggacaggaat
    60541 ttattgatga acaggacatt gcgctgcaag ccgagcgctt ccatcaattc aaccagcatt
    60601 tggcgatatt tttcaccttc ctgctgcaac agcatgggat gggtctgtcc cagaatgagg
    60661 tagattacgt caggatgatc ggttaccact tcagctatcg ctttgaggcc tatttccagg
    60721 cccttgcccg gccctatcaa accaaatgtg ctgatcaagc ggtggttttg caacccatgc
    60781 tgatgcttga gttctggctg ggcataagga acgaaatcgg gaaccccatg ggctatgact
    60841 tttacgattt ctgccggtgc ttcataaaga tcaatcagta agttgcgcga acgctgtgtc
    60901 atgcacacga taccggctgc ccggtgacag gcctcattta ggatttgttt ttgctttttg
    60961 gatggacggg gcagtacggt atgagtgatt agaatatatg gtttgtgcag cagtttgatc
    61021 agctgtaaaa catattctcc gtcctgtccc ccaaagattc cgtactcatg ctgcaggatg
    61081 accagatctg ctgcagcagc gttgatgaag gcagccgctt ttaaataatc ctggcgctgg
    61141 ttttgccgga tattgaatat gacctgattg ctgtaatgat agttgcagcc atggtcggaa
    61201 atggcgacaa tattgagatt gtgcccctca gcggtgagac tgctgcataa atctgaggaa
    61261 aatgttgcaa tgccgcactg tttggggagg taggtgccga tatttatgat attcatgatt
    61321 gtttctcctt ctttttcaag ctgtcgatta agttcctgta agtattctgg catcatttgc
    61381 agtggactat acctgctgga tggattcttt aaaatgctgc ttgcggtatt tttaagccca
    61441 aggagcctag ataaccaaat accatactgg ttatattttc caatacctca ggagagtccc
    61501 cttcacagcg gcaaataatt tccggaccgg tattggaagc ccgtaccagt ccccaaccat
    61561 ggggaaagat aatccgcgct ccatcaatat caatgacttc atgatctttt gtaaaatgct
    61621 gccggacctg ttccaccaac tgaaatttgc tcttatcatc gctggggaga cgaatttccg
    61681 gggtggcata atacttggcg gtatcactca gcatttcgct gatggtctgc ttgctgttgg
    61741 ataagagcaa cagcaagcgg gcggccgcat aaagcgcatc atcgaaccca taatagtcgt
    61801 cagcgaaaaa catatgtccc gacatttctc cggtaaaaat agcccccgtt tctttcattt
    61861 tggcttttat caaggaatga ccggttttat agagcaacgg cgtgccgccc agtcgtctaa
    61921 tctcgtcgat gagactttgc gagcatttaa cttccacaat gctcggcaga tgaggatagc
    61981 gcggcaggat atcacgccag aaaaggatca tcagctgatc accccagatc atgttgccat
    62041 cccgatccac cacccccaaa cgatcgccgt ctccgtcaat gccaatgccg atgtctgcct
    62101 gggtttgctt gacgagatga atcagatcca gcaggttttc aggtttgacc ggatcgggat
    62161 gatgatgggg aaaagttgga tcagattcac aatacaaggc aatggtttcg caacccaaac
    62221 gtgtaaacaa ttggggggca aaaggagatg ccgaaccgtt tccgcaatcg accacgactt
    62281 ttggtttata gggacccagt tttatttttt cggtgatcat ttgcatatac gctgtgcgga
    62341 tgtcgtggtt gtttaaactg ccgcgtgact gtgcggggaa atccttcttt tcagccaatt
    62401 gcttgattgc cgtaatttga tcgccataaa tggttgattc ccccagcaac agcttacagc
    62461 cattgtattc cccgggattg tgactggcgg tgatcataat gcctgccccg atctccagat
    62521 gacgggatgc ataataaaac atgggggtga tcaccatccc cagatcggtc acctcacagc
    62581 cgctttgcag caaagcttcc gttagcagat cgcggtaatg aggagaggaa atacggttgt
    62641 cccgccccaa caggattttc cggtaaccgt gctggcgggc gtaaagtgca aatgcacgac
    62701 cgatgttaaa cataacttca tctgtcagtt cctgaccaac gatgccgcgt atatcatatt
    62761 gccgataaat atttgaactg ctcatttttt cctcccctgc gattcttaaa gcaagtcatg
    62821 gcgctcaagg gtttcgatct gccttaccag ctgactgata tcctggctgc gatggcggtc
    62881 ggcaaccaga ataacatcat cggtttccac gaccattaca tcttccacgc caaagagaac
    62941 tacagttttg gtgttttgtt tgaccagaca gtttttgctg tccacaaaca agatatcctg
    63001 gcctaataaa taattctgtt gctggtcata ggggaacagc tctgccagcg cattccaatt
    63061 gccgatatcg gaccagccga accgacccgg cacgacggta acgcgctggc ttttttcagc
    63121 gataccgtag tcgatgctga tattgggcaa tgcactgtaa tggcgcaagt aatgttcgtg
    63181 gccggactgc agatgcataa atatatccgg cagaaaacga gcgaattcct ctgccaggat
    63241 tcctactttg cctacataga tgcctgaatt ccataaatac cgcccgctat ccagatacat
    63301 ggcagctgtt ttggcatcag gcttttcacg aaaagaaagg acatcaaaca catcttctaa
    63361 atcattgcac tggcggtccg atttctcaat gtaaccgtag ccggtctcgg catgatcggg
    63421 ttcaatgcca atggtaacaa gatggttttg ttccgcagcc tgtacagcgc ggtgcagcgt
    63481 ttgacaaaac gaagagatgt caaaaacatg atgatctgca ggaaaaattg ccagtacttc
    63541 gtccgccagg gcaggataga agcgtgacag cgacaaaccg attgccggcg cagtatttct
    63601 tccctccggt tcactgagaa tttcgatttt atccgcatcc gggcgctgtt ggatgatact
    63661 cttaatatcc gccattaaat tatcattggt aatgatcaga agctgatcaa ctccgctggt
    63721 ttccaggcga tccagagtct cgtccagcat cgatttgctg ccggtcaatt tacatagttg
    63781 tttgggttgt ttataacggc tttcaggcca taagcgcaag ccacgtcctc ccgccaagac
    63841 tacggcaatc atttacaata cctccgtgca tgaaccagtc gggactctga agaacatcgt
    63901 cgaaagaatt tgctcaatca attatattag cgttcctgtc aacccgctaa tatgataacg
    63961 gtaaacagcg gctgcaataa ttactggtcg cggatttcac tcgagcgaat gcaattctag
    64021 attattatgg acaaacaagc acagaaatat aagtgaaacc caaaaatctg ccgaaataca
    64081 cagacggttt tttttcacga tgctgtatta tatttcccag tcaagaaaaa tatgctgcag
    64141 attattgctc ggaaagggcg aacggaccct gtagttgttc cggtttgggg ccgaaaaact
    64201 gaaaataatt acactggatt cgaccattgt acagcttgcg ctttttatgg gccttgcgct
    64261 ggaagagttc ttcaaagcgg gggtgggagc aaatgatata gattgaccaa gtatcaagac
    64321 gggagaatac cttacccatg atccggtaaa gcgcttccgc ttcattttga tctgccagcc
    64381 gttcaccata tggaggattg gtgatcagat agccgtattt gtatttggag cttagctgac
    64441 tgacgtcccg ttgctgaaag gacagcagat tttgcagacc ggcctgtttg gcatgaaaac
    64501 gggccaaatc aaggacagaa acgtcctgat cgtaacccat aatccccaac ggctgatggc
    64561 gaaggatcgc atcctcagct tcttcccgag cctgacgcca catcacagcc ggcaattgag
    64621 gccagttttc agcggcaaag tcccggccca ggcccggcgc catgtttctg ccaattaagg
    64681 ccgcttcaat cggaattgta cccgaaccgc acatgggatc gatcaaagcc cggtcggctt
    64741 tccaacggct gagactgacc aaggccgcgg ccagcgtttc cttgagcgga gcttcagcgg
    64801 agagcttgcg ataaccacgt ttatgcaaac cggctccgct ggtatcaatg gttagggtaa
    64861 cccggtcttt caacagggca acctggattt tatacttggc accgtcttcg gaaaaccatt
    64921 gacgagggta acgctgtttc attttttcaa ccacagcttt ttttacaatg gcctggcaat
    64981 ccgggacgct gaatagtttg gattgaattg atttgccttc tacaggaatc gcggcatttt
    65041 cgggcagcca ttcatcccag ggcagggctt tagtctgctc gaataattct tcaaagctta
    65101 aggctttgaa ttctccggct tttattagca agcgatcagc tgagcgcagc cagagattgc
    65161 agcgggggat ggcctcaagt ccggcttcga aatcaaccct tccgttttca actttttggt
    65221 tggcatagcc taatttcttt agttcattgg ctacaatgga ctccagtcca aaggcagcgg
    65281 tggcaatcag atttatgttt tgcattatac tcgtctttca tttcagccag ggtggttcat
    65341 atccgtaccc tgcgcagcat ttgtcatcta tattacatta tttttcccgt aagaccaaat
    65401 gggttgcatg tataattatt tatggtcatg atgaaaaacc cttgacatga gaaaaatcct
    65461 ttcctaaaat aattctagaa taccaaatat aaattagggc aaagccatcg aaagagggtg
    65521 acgcaaagct aaaggggcta aggtggaatg atctgctatg ccagccagtt gcaaacagat
    65581 aattcctccg caagtttaaa cagtttacaa caaacccaac tgaacaaagg caaagccttc
    65641 gaaagaaggt gacgcaaagc taaaggggct aaggtggtca tcccgctatg ccagccagtt
    65701 gccgaggagg gatttatcta tggaggatga atgacgtttc gacgtcattt tcaaaccact
    65761 cgtcaatggt gcgagtggtt ttttgcatgc tatggggaag aacagatttt gcatccatga
    65821 ataattatga agaaagaagg aaagaaaaat gaatttaatg atgagattgg ttaaagaaga
    65881 agatggccag ggaatgactg aatatgcttt gattattggt ttcgtagcgg tggctttgat
    65941 tgttaccatg attgcgttta aagataaaat cgctgatttc tttgatagcc ttagtttcac
    66001 tgctccggcg gcataaaaga aatgaaaaat tagttcatta tatccttgtt acagaaaatt
    66061 tagcaagtca tttttatgga cgctggatcc aagaacagac cctgctccta tatcaggcag
    66121 ggtctgttca acaaaggagg actggatttg caggacatcg ttttggcatt catgcttttg
    66181 gtttgcctgt tcacagattg gcgggaaaga aaaatttaca atttgatcct aataccagtg
    66241 ttttgctttg gtctggcctt ccatttagtc actgctggcg gtcagggact ggtccattcc
    66301 ctggcgggct ttctgaccgg tttaagtatt ctgatcatcc ccttcgccct gggcggtatg
    66361 ggggctggtg atgtcaagct gctggcagtc attggcgcag tcaaaggccc tgtgtttgtt
    66421 ctgtataccg ctgccggcat ggctctggcc ggcgccatga ttgccttggc gatcttgctt
    66481 tatcagcgtc gcttgctcag cttgatggca ggattggcaa gagggttgca agtattgctg
    66541 gtgacacgtt ttcaagtagt tgcctttaac ctcaaccgcg aaaaaattat gtttccctat
    66601 ggactggcca ttgttgccgg cgcagttggt gccttctggt gggggggatg atttgtttgc
    66661 gacgcagata taccaaccaa cgtggacaag ctttggtgga acttgccttg atattaccgg
    66721 tattgttgct gctggttttt gggattattg aatttgggag gatttatgcc agcaatctta
    66781 tgatcagcaa tgccgcccgg gaaggtgccc gggcagcatc gctgggtgta cccgatgaag
    66841 atattgtcat tattgttaaa gacagatgtg cttttttgga tgccgccaag ctggatattg
    66901 atattacacc tgtgcctccg gaacgtgcta ccgggaatcc ggtgaatatt gatgtgaatt
    66961 acccgctgga gattaaaacc cccatcattt cagtgataac cggggatccc tgcccaatat
    67021 cagcccgggt atccatgcgg atagagtagt gatgatgaag gaaatgagaa ctatgattgg
    67081 caatgaaaaa ggcagcgcca ttgtattgat aactgtttta atgaccgttc tggtggctat
    67141 ggcagcgatg gttacagata ccggtgcaat tttattaacc cggtcccaac tgcaaaatgg
    67201 tgtggatgca gctgcgctgg cggcattgca ggatctgccg ggggatgctg cccaagcggc
    67261 agcaactgct gttagttatg cgaccgctga ctgcccgccg caggcagaaa ttgatacccc
    67321 acaaattctc gatgctaacc gcgctgtttt agtatcagca cgcaaaacag ttcattatgg
    67381 cctggccaga atgattggac tcgatgccaa aacggtagaa gcccaagcac tggctcgttt
    67441 ggaaccgatt accggggtca gcggggcgct gcccctggcc gtcatgaaag atgtctggat
    67501 tcccgggcag accgttactt taaaaggtgg tgcccaagac gcaattttca gcggctggcg
    67561 cggtgccctc gcgctggggg gcaatggggc cagtaattat gaagagaatt taaaaaacgg
    67621 ttatacaagt attctccgtt tggatgattt ggtagatgta gaggaaggca acatgagcgg
    67681 gccaacgcag tccgggacag agtatcgatt gggcggatgc ccccataccc ccgtttgtac
    67741 aatggaacat tatcatacaa actgcccgcg tgttggcgtc ataccggtgg tggaggaagt
    67801 attgctgacc aataaaaaag gagagcagac cacatccagg aaacaggtac gcatatgtgg
    67861 gtttgcactc tttctgctgt ccgatacaca aggcaatggc agcgatggcc agatcaccgg
    67921 acagtttatt cagggaatca ttcaaggaga ggccgacccc gacggaccgg attacggtgt
    67981 atacagagcc cgactggtcc agtagtaatt ccgaggagag ttgcggatga agagcaaact
    68041 tgtatttata ctggctttga tttttgggtt gatagccgcc tatctgacct ttagctattt
    68101 aaaccagatg aaagcgtcga tggataaccg tgagtatatc cggatcgcag ttgcagcccg
    68161 ggatattccc gctgatacca cgatcagccc ggaaatgttg cagatgaaag cctttcccag
    68221 tgaatttaga aataacctgg aaatacttga tgtcaacgag gctgtgggga gggtcgcgtt
    68281 gaccgacttt caccaggggg aagctattct taaccatcat ttgattaaga gcggcgaacg
    68341 caaatatggc ctggcttatg ccattagcag cgggatgaga gcgatggctg tgaacgttga
    68401 tgaagccagt ggtgtagccg gactgattaa agtaggtgat cgggtggaca ttatcgccaa
    68461 actgaatagc gaggaaggtt caatgctgcc ccgaacggtt gttgtactgc agggggtgga
    68521 agtattggca atcggaagca gtctggaaaa cgccaagtcc gacaacaagg aaaaggccat
    68581 tgtcaagaca attaccatgg cagtgacact ggaagattcg ctgcggctga agactgcggt
    68641 ggaaggcggg aaaataagct tgatcctgcg ctcaccgtct gacaagggtt tcagcaatcc
    68701 ggcagcattc agtgccatcg gttttcaggt agatgttgcg cccctggctg gcagttgata
    68761 aatcaaccat atgcaacagt tgcaacagca attatgggag taatgttttt atgcttgtgg
    68821 aaggagagtc caacgtggac aaaatcagaa ttctgatggc cagcaacaat caagcttata
    68881 gagaggcgtt gacttccaat ttatctcacg aaaacgatct tcgaattatc ggagaagtgc
    68941 cggatggcat ccaggccatt aaaaaaacag aacagctctt gccggatatt gtagtgattg
    69001 aagttgattt gtcttcaggc caagagatta cggcagttga aaaaataacc ctggctcatc
    69061 ctgaggtagg ggtcatcgtc atcggcctgg aagacgacag tgaatctttt cgcaaggcga
    69121 tgatggcggg ggccagagat tattttgtca aagaacagct cagtgccggc gaactggcgg
    69181 aagcgattcg cagaatattt ctgacggaaa agacccgatt ggccagctat gcttccgtac
    69241 gcagagccga gcagcacaat aaaaacaaag ttccccagat cgtaactgtt tttggggcca
    69301 aaggaggagc gggcaagacg accctggctg ttaatctggc ggtgatgatc gcccagcaaa
    69361 gcaaaaaacg ggtagctttg cttgatctgg atttgcagtt tggcgatgta gccgtttatt
    69421 tgaatctcaa gccccgacgt acgatggccg aactggtaca ggaacgcaat caatgggaca
    69481 tgcagctgtt aagcaactat cttgtccctc acagctcagg cattaaagtg atggcatccc
    69541 cgcttcgccc cgaagatgca gacttgattg taccggagca tgtggagaaa attctttccc
    69601 ttctgcgaga tcattttgat tatattatta ttgattcccc gccgtttttt tcagatacca
    69661 ttctggctgc tttggacatg tctacgcaga tcgtcttgct gatgtccatg gatctccctt
    69721 ccgtaaagaa tctcaaactg agtttgaatc tgctggacag cttgcatcac agcggcaaaa
    69781 ccaagttggt catcaatcgt tcttccatga atttcggcat tgatattcac gatgtggaaa
    69841 aaaccattga ctttttagct gcaaaagaac ttcccagtga tggtcagacg gtagttggtg
    69901 cagccaataa aggcacacct ttcgttctct cccatccgca gacggaggtc agcaaagccc
    69961 tgctgcgaat cactcgtatg atcatcgaag attatggtta ccaaaaagac ctgcgagaaa
    70021 ggaaaccaaa gtcatccttg tggtcgaagt tatttggata ggagtggcgg catgtctctc
    70081 agtcagcgat tgcagaataa aaacgcatta ggtgatccgg aaaatataac agtgcccgac
    70141 agcaaacatg tatgcgaaga aaaggcggac aagtatgctg tgttgaaaca gcggatccat
    70201 aaagaagtaa ttgaaagaat cgatcgcgag ataaagcaag cggaactgag cgatgctgtg
    70261 cttacccaaa aaacccgtga aatggtgaac agcattctcg aacgggaagc tggttttctg
    70321 ccgtattcag agaggcagat gattgccgcc gttgtgagtg atgaagtact gggcctgggg
    70381 cctctcgaac cgcttttaaa cgataatgac attacggaaa tcatggtcaa cggccctaaa
    70441 caaatttatg tggaacgcca agggagactg gaattgagtg aaaccaggtt tcgtgatgat
    70501 gaacacgtta tgcatatcat tgacaaaatc gtttccccca ttggccggcg catcgacgaa
    70561 agccagccca tggtggatgc aaggctgcca gacggctcac gggtcaatgc catcatacca
    70621 ccgctagccc tgaagggacc cagcataacg attcgcaagt tctccaggac accttacaat
    70681 attcatgacc tggtgcgctt tggcagtctg accctgccca tggccagatt tttagaggca
    70741 gctgttagag gccggctgaa tatatttatt tccggcggta caggcagcgg caagaccacc
    70801 ctgttgaatg tgctggcttc ctttataccg gaagatgaac ggatcatcac tgtggaggat
    70861 gcggcggaat tacagctcaa tcaggcacac gtggtcagcc tggaaactcg tgctgccaat
    70921 atcgaaggca aaggagcagt gaccatccgt gacctggtac gcaactgtct gcgaatgcgc
    70981 cctgaccgga tcgtggtcgg agaggtgagg gccggggaag cactggatat gctgcaggcc
    71041 atgaataccg gacatgatgg ctcgcttacg accggtcacg ccaactcccc ccgtgacatg
    71101 ttggcccggg tggaaaccat ggtgttgatg gcaggaatgg atctgcccat cagagcaatt
    71161 cgccagcaaa taacttccgc ggtagatctg attattcaac agacccgcct gcgcaatggc
    71221 cgtcgcgtca tcacccatat tactgaaatc ctggggatgg aaggcgatac gattcaaatg
    71281 caggatattt ttctatatga acaacgtggg atagatgcga agggcgagtt gttgggacat
    71341 cattttgcga ccggaatcac accccgactc ctgagcaaat tagccgcaga ggggatcact
    71401 ttgacgaccg atctgtttcg cccggaaaac tgaggcaaag gggggatata agtggcagca
    71461 gcaatcagtg tgttggtttt tctatcgggt tttctgttca ttatgggtat ttatcaattc
    71521 agcacacgtc atcgtaacaa tataaaaaac cgggttctga gtcttaatga aagaagcagc
    71581 gatgcaattg aggcaacgtt tgtcagcaaa caaaagtggg gctacaaaaa tttgctgcgc
    71641 aggatcggaa gtgtttttac agctcgatca tggggccgca gattggaagc ggagttgtca
    71701 aaagcagaca tgatgctgcg tggagaagag tttgttggaa tgaacattct ggtcacaatt
    71761 gttggcggtt tgttaggttt cctgttgttc ggcgggatta gtcaggcgtt gttaattggg
    71821 gggatttgca tgttattccc ggggatgctg gtcaaacata aaaagaaaac ccgtttaaac
    71881 aagttaaatg agcaaatcag cgattccctg accgtattga ccaactctct gcgcgccggt
    71941 tacagttttc agcaggctct tgaactggtt ggacgcgaga tggacggacc gttggccctt
    72001 gaattcagaa ggacactgcg ggaaattcat ctcggtaata ccaccgaaca ggcactgata
    72061 aacctaatca aaagagcaca aagcagtgat ttggaactga tgctgaccgc agtccttatt
    72121 cagaggcaaa tcggcggtaa tctggccgaa atctttgaca atatctccaa cagtataaaa
    72181 gaaagaattc gcttaaaagg ggaagtgaaa acgctcaccg ctcagggaag aatctccggt
    72241 ttgctgattg gccttttacc ggtaggcttg tttgccattc tgattttagt cagccccgat
    72301 tatataggcg ttctggtgcg ggagaacagc ggtcgcatga tcctgggggc ggcagtggtg
    72361 tccgaggtga tcggattctt aatggttcgt aaagttgtcg atattggttt gtagggagaa
    72421 gcgtatatgt taatgattat ggcagtgatt tttctaagtg ccttctgttt gacctgggct
    72481 gttttggtac gcttgttggc aacccgcaac ctgatcagca gcagattgga agaattaaat
    72541 cgttatacgc ctcaggctga tagggaggat gaacttcaag tagcctttca tgaaagagta
    72601 cttctgccca tgttgaagaa aacatcgttg tttacacaga agtacactcc gcagcgacaa
    72661 agagaaagca tcgaaactaa actgttggca gccggacacc cttttcgttg gactgctgct
    72721 gactttttat ctgtgcagta ttcgaccgca gtcgtcctgg ccattatggc agtgctggcc
    72781 agctattttt cacatgcaag ttcaggtgta caatatctgg ctaccattgg cggatttgca
    72841 gtcggttata tgctgttgtc gctgctgtta aattccatta ttgccaaacg gcggaccaat
    72901 atggaaaagg aattgccgga tttactggat ctcatggtta tcagtatcga agcgggactg
    72961 gggtttgatg cagcgctgca aagggtggtt caaaagtcca aaggaccatt gtcgcaggag
    73021 ttcaaccagt gtctacagga aatgcgtatg ggcaagaccc gtaaagaggc gctcaaagat
    73081 ctggtcagac gtaacgagga aggcgatctg tcagctttcg tcggagctat cattcaggcg
    73141 gatcaattgg gggtcagtgt tggcaacgta ctgcgcaccc aagctgacca actgcgggtc
    73201 aagaggaagc agaagatcga ggaaaaggct atgaaagctc cagttaaaat gctgattccc
    73261 atggttttgt ttattttccc gacgattttc ctgattctgc tgggaccgtc cgttatccag
    73321 atgatggaga cttttaaatg aacgacaaac agtgttgccg ggttataaac catgagaata
    73381 aaagaataat tgcgcctgac attgaagtcg ccggaagctt ttttccccgc ttgcgcggtt
    73441 tattgggaag gaaaacactg tccgccgggg aaggcatgtt gttgtggcct tgtaatgcta
    73501 tccattgttt tggcatgcgc tttcccatcg atgcgatctt cctggatcac caatatcggg
    73561 taacccatat aaaggaaaac ctgacaccat actccctggc ttcagatcgg caggcaagcg
    73621 gagtgttgga actgaaggcc ggggagagca gaaagcatgg gctcaatgtc ggggatcaat
    73681 taatcatctg ttttgcagcg ggtgcagttc ccgataaaga agcaagagat aaagaaaaat
    73741 gcggtcgtcg ttgaccgcat ctcttctgta caacccagta ttaatccctg tttttactgc
    73801 gactgccgga ggctttgtcg cgctcaccct tatccaggat ggttttgcgc agacgcaatg
    73861 atttcggggt tacttccagc agttcatcat cgctgataaa ctcgagacat ttctccagcg
    73921 acatgttgac cggggttatc aaacgcagcg catcatctgc tccggatgaa cgagtgttgg
    73981 tcagatgttt gcgtttgcag acattgacgg taatgtcaac gttgcgggca ttttctccaa
    74041 caaccatgcc ggcatagact tcggttcctg catcaataaa aagtatgccc ctttcctggg
    74101 cgttatataa tccgtaagtg atcgctgtgc cggactctga agcaatcagc gagccccgac
    74161 tgcgggtgct gatgtctcct ttataaggct cataattatc aaaggtatgg ttcaagatcc
    74221 cgttgcctct ggtgtcagtc ataaattcac tgcgataccc aatcaggcca cgggcaggga
    74281 tgagaaattc cagcttcatc acactaccgg taccgttcag catggtcatc agttcccctt
    74341 tgcgtttgcc gaccccttca atgaccgtgc ccacaaactc ttcggggact tcaataaaca
    74401 acctttccac cggttcatat actttgccgt ccatgttgcg caaaatgacc tcgggacggg
    74461 tgacggcaaa ctcataccct tcccggcgca tggtttcaat caaaatggaa agatgcagct
    74521 cgccgcggcc ggatacctta aaacagtccg gtgttatctc ttcaacccgc agcgaaacat
    74581 tggacaataa ttctctttca aggcgctccc gcagatgccg actggtaaca tagctgcctt
    74641 ccagccccgc catagggctg gtattgacca caaaattcat ggagatggta ggttcatcaa
    74701 tgtgtaaaaa agcaatcggt tccatcgcat caggactgca caaggtgtcg ccaatggtga
    74761 tttcttcaat tcctgaaaca gccacaattt caccggcctt ggccgaagta gtctcaactt
    74821 ttttgagccc ttcataagaa taaagggtat tgatcttaac acggtcctgt ttgccttccg
    74881 aattgcacaa gatagcttcc tggtttttat tgatcgtacc attaagaatt ttacctaccc
    74941 cgatgcgacc gacataggta tcataatcaa tagaagagat aaaaagctgc aaaggagctg
    75001 ctccgtcgcc tgcgggggca ggaatttcat caatgatcat atccagcaat ggtttcatat
    75061 cttcattggg atcattcata ttaatcttgg caaaaccacc tttggctgaa gcaaaagcga
    75121 caggaaattc gagttgggat tcatctgcgc caagatcaat aaagagatcc aacaattcat
    75181 ccaagacttc atcaggacgg caatccgggc ggtcaatttt attgatcacc acaattggtt
    75241 tcaggccagc ttcgagcgct ttttttagta cgaaccgagt ctggggcata gggccttcaa
    75301 aagcatcgac cagcagcaaa accccgtcaa ccattctgac gattcgctca acctctccgc
    75361 caaaatcagc atggccaggc gtgtcaacaa tgtttatttt aacatcatta taatagatac
    75421 tggtgttctt ggagagaatg gtaatgccac gttctctttc cagatcattg ttgtccatta
    75481 cccgttccgg cacaaattga cgatcccgaa atgaaccgct ctgcttcaac agtttatcaa
    75541 ccaaggttgt tttgccatga tcaacatggg caataatagc gatatttctc agtaactttt
    75601 gagacatttt aaaaaccctt tcccaattaa aatgattaat ttgtttaaaa gcaaaaacta
    75661 cattatatag aaaaaacgaa tttctgctgc agaacggaaa cattttcttc ctacaaaaac
    75721 aacattccta ttatagcaag gggatttaat cattacaaat attatagaag taaagatgaa
    75781 tcttaatcag tatttcgtga ctgccatatc ggacgaatat ttatattcgg cagtaaatcg
    75841 tgtcaaaata acggtgaaat tttttcgaat aatgactgac gaatgaattg aaagcaagga
    75901 gcggcatgct gcgtatgcct ataatcagga cacgtagcct ggtgtactaa ttcaaaaaaa
    75961 caactaaatt acgttgacag ttgtcgaata ggatgataat ataactcttg cgcggaacga
    76021 gagggaagcg cgaggatctt gataaaa
//
LOCUS       DAOTHZ010000002        70886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_340_length_70886_cov_7.516186, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000002 DAOTHZ010000000
VERSION     DAOTHZ010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 70886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70886
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_340_length_70886_cov_7.516186"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="P5549_00360"
     CDS             complement(<1..284)
                     /locus_tag="P5549_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HRW11556.1"
                     /translation="MKVLVCVKQTFDTEAKIELKDGKINDAGINLIINPYDEVAVEGA
                     IQLKEKKIATEVVVVSAGADKAMDAIRTALAMGADRGILVKQDTAADEYA"
     gene            complement(631..1413)
                     /locus_tag="P5549_00365"
     CDS             complement(631..1413)
                     /locus_tag="P5549_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HRW11557.1"
                     /translation="MQKDVYDWEWFVKKFETISRIDLLAYKRPQMERRINSFMKTVEC
                     PDYKSFVSLLEINLPVRQKFIEYLTINVSEFFRNPDHWKVLEERIIPQLLTHRTKLKV
                     WSAGCSTGEEAYSLAMLFHAKFPGHVQKIKATDLDQEVLRKAIQAVYTGKAFKTLPPG
                     YLHRYIQSDGDHYCVIEEIKRMVEFARHDLLRDKFELDHDLILCRNVFIYLTEESKQD
                     LYRNFAQALRPGGVLFIGSTEQIFRPAQAGFQSLDTFFYQKI"
     gene            complement(1425..2711)
                     /gene="hemL"
                     /locus_tag="P5549_00370"
     CDS             complement(1425..2711)
                     /gene="hemL"
                     /locus_tag="P5549_00370"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRW11558.1"
                     /translation="MKTTRSEQIYRQALKVMPGGVNSPVRAFQSVGMDPLIMKRGSGC
                     RIYDEDGNEFVDYVGSWGPLILGHSHPAVVEAMIAAARDGSSFGATCAREIELAEMIC
                     EAFPSIDKVRMVNSGTEATMSAIRLARAYTKRNKIIKFEGCYHGHADTFLLKAGSGML
                     TGGSQSSPGIPRSISEHTLLCKYNDIESVKKAFMQYGPDIAAVIIEPVAGNMGLIPAE
                     MEFLQTVREVASMYDSILIFDEVITGFRLCYGGIQNLTGITPDLTTLGKIIGGGLPVG
                     AYGGRKEIMDLVAPLGEVYQAGTLSGNPLAMAAGIATLKELQDGDVYDRLEVLGYVLA
                     GKIRTLFEEHNRFYSIKRLGSMWCVFFTEEEVRDYESVQCCNTEQYADFYRNLLHCGV
                     YLPPSQFEVSFISGAHDLTDIELTVEAMGKALNMMK"
     gene            complement(2757..3230)
                     /locus_tag="P5549_00375"
     CDS             complement(2757..3230)
                     /locus_tag="P5549_00375"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRW11559.1"
                     /translation="MMYEDEKIRRIIQFLQGDIPLESRPFQKLAVDLEISEEEVLERI
                     QDLQKQGVVRRWGAVLRHQQAGYTCNAMVAWKVGPIEADRAGEAMAEFHEVSHCYLRE
                     VSADFGYNLFSMVHARSQTDLAETISRIADVTGLDDYVVLNSIRELKKVSMRFFD"
     gene            complement(3227..3688)
                     /locus_tag="P5549_00380"
     CDS             complement(3227..3688)
                     /locus_tag="P5549_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HRW11560.1"
                     /translation="MKLDQLDRALLDALQNTLPLSCQPFGDLAARLKTSEEEVLARIA
                     ALKETGLIRRIGGIMNSKQLGYTSALCAMTVPEERIDEVAAILNQLSGVTHNYVRDHH
                     YNLWFTLTSPSQAALIQQLKALEQACGLSILPMPARKLYKIKVSFDMGAES"
     gene            complement(3685..4686)
                     /gene="nirJ2"
                     /locus_tag="P5549_00385"
     CDS             complement(3685..4686)
                     /gene="nirJ2"
                     /locus_tag="P5549_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative heme d1 biosynthesis radical SAM
                     protein NirJ2"
                     /protein_id="HRW11561.1"
                     /translation="MLVSWNTTNQCNMFCDHCYRDAGARLEDELNTSQAQKLIDEIKK
                     AGFKIMIFSGGEPLMRPDIYELGSYATRRGLRPVLGTNGSLITPDVAQKLKQAGFMAA
                     GVSLDSLIPQKNNAFRKLDHAYDLTVQGMLNLKEAGVPFQIHTTVMDWNVAELEDITD
                     FAVQIGAMAHHVFFLVPTGRGADIEEEALRVAEYEKTLARLMEKQKKVPIEIKPTCAP
                     QFIRVADKKGIQIRFSRGCLAGISYCIISPTGEVQPCAYMDMPLGNVKEIPFDKIWTE
                     NSVLQELRSMEYGGKCGICDYREKCGGCRARANYYNGSIMAEDSWCLYNPRQEGQKI"
     gene            complement(4697..5683)
                     /gene="hemB"
                     /locus_tag="P5549_00390"
     CDS             complement(4697..5683)
                     /gene="hemB"
                     /locus_tag="P5549_00390"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRW11562.1"
                     /translation="MTYPIQRMRRLRANETLRVMVRETSLTTQDLIYPLFILPGQEQK
                     HPIHSMPGVFQFSADRLPAEIKAIESLGIPAVLLFGLPEHKDETGSEACDPNGSVQQA
                     IKRIKDLCPQLMVITDVCLCEYTSHGHCGVLKADRSVDNDRTLEFLAREALSHVQAGC
                     DMVAPSDMMDGRVGYIRNCLDQNGFENIPIMSYAAKFASAFYGPFRDAADCAPQFGDR
                     RSYQMDPGNFREAMREVELDIQEGADIVMVKPALAYLDVLHEVKNRFGLPTAAYNVSG
                     EYSLIKAAAAQGWINEEAVTMEVLTAIKRAGADLIITYAAKDAARLLSGNIV"
     gene            complement(5750..7279)
                     /gene="cobA"
                     /locus_tag="P5549_00395"
     CDS             complement(5750..7279)
                     /gene="cobA"
                     /locus_tag="P5549_00395"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRW11563.1"
                     /translation="MARRGFVYLVGAGPGDPGLFTIKGKELLEQAEVVIYDRLVSSDI
                     LAWANPQAEMIYVGKESSRHTLHQEEINALLADKAAQGKMVVRLKGGDPFVFGRGGEE
                     ALYVKERGYDFAIVPGITSAVAVPSYAGIPVTHRDATSSFAVITGHEKPGKEVSSIDW
                     EQISTGIGTLVFLMGVENLEYIVSNLLSNGRSPETPVALIRRGTMPDQEVVSGRLDEI
                     VDRVREAGLTPPAIIVVGDTVALRSRLNWWETGPLWGKRIVVTRSRSQASVLVKKIRE
                     LGGEALELPTIEITEEENLDQLHRAFAQIETYHWIIFTSVNAVEIFFRELFQSAQDIR
                     SLAGIKVGAIGPATAERLQQYGLRVDLMPAEYLAEGILDGLQNVIVPGQKVLLPRAKG
                     ARDVLPLELVKRGAEVEEINLYQAVVPTCTNPGKLKAILEQPIDFITFTSSSTVTNFV
                     EMAGSEKAKALAQRVKVACIGPITADKAREKGLPVDIVASEYTIDGLLRSILADLTEK
                     D"
     gene            complement(7282..8211)
                     /gene="hemC"
                     /locus_tag="P5549_00400"
     CDS             complement(7282..8211)
                     /gene="hemC"
                     /locus_tag="P5549_00400"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRW11564.1"
                     /translation="MRVIRLGTRGSKLALWQAEYVARELSRRWEGISVEIVIIKTKGD
                     KILDVPLAQIGDKGLFTHEIENELLDGRIDMAVHSMKDLPSLLGEGLCLGAVLPRENP
                     HDVLLSTRYQCLADLPAGAAIGTSSLRRVAQLRTLRPDVQLVSMRGNVETRIRKMEEQ
                     GLDGIILAYAGVKRLGFAGHIREIIAGEHILPAVGQGAVAVEMRAGDEDTLQMVNPIN
                     DPVAWLEITAERAFLARLEGGCQVPIACLARKEADHLNIQGLISSLDGSRVYKASLEC
                     GLDDVAGNGRQLAEQLLSRGAGQVLDEIRGQGE"
     gene            complement(8177..9496)
                     /gene="hemA"
                     /locus_tag="P5549_00405"
     CDS             complement(8177..9496)
                     /gene="hemA"
                     /locus_tag="P5549_00405"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRW11565.1"
                     /translation="MYVLLAGLSHSTAPVEIREKCAFSEKQLESAYKRLTEFEEIEGL
                     VILSTCNRTELYATTRDIPSGQKVLKDFLLDYSGLSELQFQEYMYQPNCYDAIAHLFR
                     VSAGLDSMILGETQILGQVKEAYEAARYYGASDAVLNALFQKALYVGKKVRTDTKIDQ
                     HPVSVSYAAVDLAVQQLGSLQDKTVLVVGAGETGKLTTRYLISHGIHSVIVSNRSYDK
                     AMEMAAFFEGKAVFFDKIAEEMSRADIVISCTAANHCVITEDNCGNTLKARQGRPIIF
                     IDIAVPRDVDANLQAIDGVTIYDIDDLQDFVNVSYQERLEAAQAAEEIITGQMEEFNQ
                     WMASLYVIPVIASLKNTGETIKQAELKRAFNRMGTISERDQVIIQNMAASIVNQLLRS
                     PVLKMKEMAVSNQGHLYAEVVKNLFDLEVDCEKEGYNAGYKVGNTGQ"
     gene            complement(9472..10113)
                     /locus_tag="P5549_00410"
     CDS             complement(9472..10113)
                     /locus_tag="P5549_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRW11566.1"
                     /translation="MEHLYPIYLHVAGKSCLIVGGGTVAERKAASLLEYGARIKIVSP
                     AAEEGIAEWAGQERLQWIPRAFEDHDLDNVFMVFIATDDNSLNKEIADLCRQRGILVN
                     AVDDPPNCDFFVPSVLRRDSLALAISTEGRSPLFAARLRRQLEAIVTEEYGYIVNLLG
                     QFREELKQSSLDINQRKQILEQMVGSDLLDLLLDGRYEEAEERINQCMSSWQD"
     gene            complement(10143..10325)
                     /locus_tag="P5549_00415"
     CDS             complement(10143..10325)
                     /locus_tag="P5549_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRW11567.1"
                     /translation="MFGLIGNFGPWELGLILLIVLIIVGPGKLPKVGEAMGKALQSFR
                     KAKDDEFNELDEKDKE"
     gene            complement(10520..11329)
                     /gene="tatC"
                     /locus_tag="P5549_00420"
     CDS             complement(10520..11329)
                     /gene="tatC"
                     /locus_tag="P5549_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRW11568.1"
                     /translation="MFFSDLSPDDKQTIIEHLEALRKSLIISVIAIIIAAFVCFAYNE
                     QILRFIITPLHNLNEKLIVTGVTEAFFVKLKLAFFAGFCVAFPVVILAIWSFIKPALF
                     PHERKYVYILFPVTLLLFCGGIVFAYFGILPLVLKFFIYIAGSNLDTMFKVDQYVSFV
                     TAFTIPFGLVFELPVIVFFLAKMGVVTSQTLSRNRKYAILIIFILAAALTPGPDPISQ
                     MMMGIPVYILYEISVLVARLARPSEERKNKRRFFEKKPTAAEKDAAKKTDE"
     gene            complement(11342..12310)
                     /locus_tag="P5549_00425"
     CDS             complement(11342..12310)
                     /locus_tag="P5549_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRW11569.1"
                     /translation="MAGFELFQHVAEELEQVEKQLLNNIQSDLSVLENASAHLVKAGG
                     KRLRPAFALLSAKLFSNSLERVIPLATALELIHMATLVHDDVIDNSSLRRGQETVKSA
                     WGNRMSIYAGNFVFARALLLVESYHRSDIVDVLADASMRICEGEIQQMLSCYNTRQGL
                     KDYLHRIERKTALLISVSCQLGAMVMQAAPREVTALKRYGYYLGMAFQLTDDILDFTA
                     SEEVLGKPVGSDIRQGVITLPSIYAIKFDPRGQELSNLLSSPECCRHRAEEIIDIVVH
                     SEGIEYAYQVTQKYADLAIRQLQFLPDGLVCQALADMAGFISGRDY"
     gene            complement(12382..12963)
                     /locus_tag="P5549_00430"
     CDS             complement(12382..12963)
                     /locus_tag="P5549_00430"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HRW11570.1"
                     /translation="MPRYIVAITGASGVVYGIRLTETLLDLGYEVHLVVSQPGVLVIQ
                     QEMGWELGERPEQSLHSFFCQPNLYLYNNADIAARLASGSFITEGMVIVPCTMSTLAA
                     VAHGSSNNLIERAADVMIKEHRPLTIVPRETPLSAIHLRNMLALAEMGVAIVPAMPAF
                     YHQPQTIEDMVDFMTGKILDALRIPNDSFRRYG"
     gene            complement(12984..13832)
                     /locus_tag="P5549_00435"
     CDS             complement(12984..13832)
                     /locus_tag="P5549_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HRW11571.1"
                     /translation="MDKLRKFLTMIDIEHSLFGLPFAYLGAFLALMDVPSLAQLGWIT
                     LAMASARSAALCLNRLIDREIDRHNPRTADWIMASGQLSVKKVWVLVFVFFGLLFYAA
                     YHLNPLCFKLAPLAVFVLWIYSYTKRFTWWCHLIMGLAIGIGPVGGWFAISGTFDWQP
                     FVLGLAVAFWTAGFDTMYACQDIEFDRANHLYSIPARFGEKGALIFSAVFHGLAIVCL
                     FLTGMILDLGMWYYGGTVFTAVILCYEHVIVRPGQLERVSFASFKINHYVGLIIFITA
                     VMDILL"
     gene            complement(13825..15276)
                     /locus_tag="P5549_00440"
     CDS             complement(13825..15276)
                     /locus_tag="P5549_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HRW11572.1"
                     /translation="MAYKDLHEFISVLETRGELVRIKTPVDPELEITEITDRVSKAFG
                     PALLFENVKGSDLPVLINAYGTRKRMAMSLQVEDLDAIGEEINQLLQIAENVPTTMLG
                     KVKLLPRLAELSSFFPRMVKSGVCQEVVSMDPDLDQLPILKCWPQDGGRFITLPQVYT
                     RNPHTGKRNLGMYRLQVYDKQTTGMHWHMHHDGAANYRASKEMGRDIIEVAVALGGDP
                     AIAYAATAPLPPDIDELIFAGFLRKKPVDLVRCKTIDMEVPADAEIVLEGYVDLAESR
                     LEGPFGDHTGYYSLTGEYPVFHVKCITRRKNAVYPTTIVGQPPQEDCYLALATERIFL
                     PLLKFQLPEIVDMHLPMEGVFHNCVLVAIKKSFPGHARKIMNSLWGMGQMMFAKYIIV
                     VDETVNVQNVSEVMWKVFNNADPRRDTMIVEGPLDVLDHSAPLPLFGSKMGIDATRKW
                     PEEGHNREWPEDIVMSAEIKALVTSKWSEYGIG"
     gene            complement(15329..16105)
                     /locus_tag="P5549_00445"
     CDS             complement(15329..16105)
                     /locus_tag="P5549_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11573.1"
                     /translation="MITDINRYPMRGNKWMNDHFARLLSAEMQQVEVLYQEGLQIWWD
                     DLTALRECLQDDKSLQLIPAVVVLAYKQLDLDEKLTMEMASLFKTLYFANLIHHQVKD
                     EEEGQIYDQALQFTILIGDYIFGRILKLLLDIGAVEVLDNLAGMICSINEGMVMQHKL
                     GAAPMDIIKRGNAVIYETAFMTAGRLKALDEDALALYRRLGLEVGMAMELLNAGADLE
                     ARPFLEKSLALLKQFNHAYGMNHLVLQKWLQDMVGERPEK"
     gene            complement(16294..16674)
                     /gene="speD"
                     /locus_tag="P5549_00450"
     CDS             complement(16294..16674)
                     /gene="speD"
                     /locus_tag="P5549_00450"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRW11574.1"
                     /translation="MQALGRHVLAEFYGCRFEMLNDINKVQDIMINAALEAGAEVREV
                     VFHKFSPQGVSGVVVISESHLAIHTWPELGYAAVDVFTCGEQVNPWDACHYMAVEFGT
                     GDVQASEIKRGYVPVQEERLVVNI"
     gene            complement(16894..17319)
                     /locus_tag="P5549_00455"
     CDS             complement(16894..17319)
                     /locus_tag="P5549_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRW11575.1"
                     /translation="MLDLNSYLGFSFRVVMKKIDRTLSQQLDPYGVSIPQSFILYSLL
                     EEDGATLKEIGNKTLIDSSSMTVLVDKLEKDELVVRKLDSQDRRAIRVFITDKGRDIA
                     EKVSKIGIAFNSRLYDLLGEGNQKEFTHGIKNIINGLDG"
     misc_binding    17604..17815
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17851..18855
                     /gene="trpS"
                     /locus_tag="P5549_00460"
     CDS             17851..18855
                     /gene="trpS"
                     /locus_tag="P5549_00460"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRW11576.1"
                     /translation="MIPVKKRILTGDRPTGKLHLGHYVGSLANRVKLQNEYESFIIIA
                     DVQALTTNFENPANLSRDTFQIAEDYLAVGLNPEISTIFIQSMIPEIHELTMYYSMFT
                     SVNVLRHNPTVKTEAQQHGFSDMTYGFLGYPVSQAADISIFKANLVPVGDDQIPHIEL
                     TRKIVRRFNDLYQPVLVEPEALIGEVPRLMGLDGKAKMSKSLNNAIYLSDSAAEVESK
                     VKDAVTDPARIRKTDPGHPDICMVFAYHKVFNGAATEDIEKACRQGEIGCVQCKKKLA
                     GCINQLLEPMRERRIQYENNPEKVSEILQEGTRRARLTAHETMREVREAMQIDYFGPR
                     "
     gene            complement(18943..19794)
                     /gene="aroF"
                     /locus_tag="P5549_00465"
     CDS             complement(18943..19794)
                     /gene="aroF"
                     /locus_tag="P5549_00465"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRW11577.1"
                     /translation="MPSFTLAARNHSQPDTVVKIHGPRQKVCIGAGYMTVIAGPCAVE
                     NQSDYIEIAQILQSIGVNVLRAGLFKPRTSPYSFKGLGGEGLRIVEEARRATGLAVLT
                     EVMDVRDIEVLYPCIDIFQVGSRNMQNFSLLEELGRTDKPVLLKRGLAATIEEWLLAA
                     EYMLDRGNTRIILCERGIRTFEPYARNTVDIGAVPLIKHLSHLPILVDPSHATGRVEM
                     VGPVAKAAVAAGADGVLVEVHQHPEAALSDGQQSLTPDQFRSLYLQISRLAELEQRIM
                     QSPPKID"
     gene            19960..20796
                     /locus_tag="P5549_00470"
     CDS             19960..20796
                     /locus_tag="P5549_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRW11578.1"
                     /translation="MDRMIDIAICQMQTSRDKEVNLRKAVQLIKTAAPKARLLVLPEI
                     FNAPYQTDLLPAYAEPVPGPTTKMLSDLAREHRVVLVGGSIPEIAADGNIYNTSCVFA
                     ADGSMLGCHRKLHLFDIHIPDQIVFRESDVFTPGEDLPTFICEGMSFTVMICYDIRFP
                     ELARQAALNGSSLIIVPAAFNLTTGPAHWEILMRTRALDNQVFLAAASPARNPAASYQ
                     AWGHSLIVDPWGTILAEAGTGEEIIYARIDPAKLAKVRAELPLLQHRRPELYRNISRK
                     EC"
     gene            20799..21212
                     /locus_tag="P5549_00475"
     CDS             20799..21212
                     /locus_tag="P5549_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRW11579.1"
                     /translation="MKFHTEYLWFNTEKRRQYICITERVREACQKSGIQEGMILVSAM
                     HISAGVYVNDREDGIIQDLDEMLENIAPLGKDYLHHRTGEDNGDAHLKSILVHHQIIV
                     PVTGGDLDLGPWQEIYYAEFDGRRRKRVIIKVMGE"
     gene            complement(21213..21578)
                     /gene="aroH"
                     /locus_tag="P5549_00480"
     CDS             complement(21213..21578)
                     /gene="aroH"
                     /locus_tag="P5549_00480"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRW11580.1"
                     /translation="MTVRGIRGATTIASDQQAEVLAATTDLLTQMQQANDFSVQDLAA
                     VLFTVTTDIHSVFPARAARQLGWDRVPLMCFPEIEIEGALPLCIRVLILWNTDYSQEQ
                     VRHVYLHGAKILRPDLQSD"
     gene            complement(21605..22021)
                     /locus_tag="P5549_00485"
     CDS             complement(21605..22021)
                     /locus_tag="P5549_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HRW11581.1"
                     /translation="MEVGSKMVATAAIKMSLSLSRQEEGILKKSLLEEFAIRAVAVDY
                     GGEFITSAAKIVERAVVAAKREGVIKDIHADEGAVAGATREALAQIMPKALGLNVGGK
                     IGIARHGDHISVAIFFGIGLLHLDEVAIGLGHRAVA"
     gene            complement(22047..22559)
                     /locus_tag="P5549_00490"
     CDS             complement(22047..22559)
                     /locus_tag="P5549_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11582.1"
                     /translation="MIEKMLEEMVNMLHKAETLLMRLVVLCLVAVVVAQGLMTDDATR
                     FYMSWSERMEGQELPAPVVANQDLYLTTIEDDLSAAQTLLTIKLVQAQSAPAAKVLIN
                     GKSLYHFSRSQVTLRVNGGDTVEIDTRNCSQPLTFAIDTAAGNLAYPKPGQVFSGNQS
                     IVMIGKIIVK"
     gene            complement(22625..23374)
                     /gene="larB"
                     /locus_tag="P5549_00495"
     CDS             complement(22625..23374)
                     /gene="larB"
                     /locus_tag="P5549_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRW11583.1"
                     /translation="MNEKELYRLLTRLQQGELALEDVYDKLKNLPYEELGFAKLDHHR
                     GLRDNFPEVIYCPGKSLAQIEQIFGRLAEQSDHVLATRAEEAVYHRIRESIPQVKYDD
                     VARLLYLCRSDLPCPGQVAVISAGTADLPVAEEAALTLEIMGVKVERIYDAGVAGIHR
                     LFDKMPMINASQAVIVVAGMEGALASVVGGLTSRPVIAVPTSIGYGASFQGLAPLLAM
                     LNSCASGVAVVNIDNGFGAAAMALAILKSRD"
     gene            complement(23507..24052)
                     /locus_tag="P5549_00500"
     CDS             complement(23507..24052)
                     /locus_tag="P5549_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRW11584.1"
                     /translation="MEKQILFAGFGGQGVLSMAQFLAYAAMEAGKFVAWVPSYGAEMR
                     GGTANCLVTIADEEISSPITENPLMAVIMNRPSLEKFEQKIQPGGTLVLNSSLVDRQP
                     QRQDVEVLSIPVNQIAEDIANPRGANMILLGAYLQKTEVVETEKVLEYFERIYKGKKP
                     SVIESNKEAFLTGVSYARQHW"
     gene            complement(24053..24796)
                     /locus_tag="P5549_00505"
     CDS             complement(24053..24796)
                     /locus_tag="P5549_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRW11585.1"
                     /translation="MKVVAKRPASLKENKFHYCPGCTHGIIHRLVAECLDELELREET
                     IGICPVGCSVLAYDYFNCDMLEAAHGRAPAIATAIKRLLPDKTVFTYQGDGDLASIGM
                     GEIIHASLRAEKITVIFVNNTIYGMTGGQMAPTTLLGQVTTTSPYGRKKEWEGYPAKM
                     AELLAQSEGSAYICRTSVHNPANVMRTKKAIKKALQIQQAGLGFTMVEILSTCPTNWG
                     LGPLDALAWLENNMIPYYPLGEFKVKEEE"
     gene            complement(24796..25851)
                     /locus_tag="P5549_00510"
     CDS             complement(24796..25851)
                     /locus_tag="P5549_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRW11586.1"
                     /translation="MQKVLMKGNEALAEGALIAGCKAFFGYPITPSSEIAEYMARKFP
                     KLDGVFVQAESELAAINMVYGGAATGHRVMTASSGPGISLKQEGISFIAEAELPCLIV
                     NISRGGPGLGGIQPAQSDYFQATRGGGHGDYRVIVLAPSGVQEMMDFAIEAFDLADRY
                     RTPVMIMAEGTLGQMMEPILIREPVQQFYEKPWATTGCQGRAPNVINTLHLSPEFLEE
                     RNHHLQSKYDEIMRREKRAETYLTENADLVVVAFGLMARVVRRAVMDARKKGLKVGMI
                     RPVTLWPFPDEPIIKAAQTARRFLSVEISMGQMVEDIRLAVAGKAPVDFYGRIGITPN
                     PEEILREIERIAEREGF"
     gene            complement(25853..26071)
                     /locus_tag="P5549_00515"
     CDS             complement(25853..26071)
                     /locus_tag="P5549_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW11587.1"
                     /translation="MAKGRVYFHIDQCKACELCVAFCPQGILGLDEKVINILGYHPVS
                     ILRAEECTGCGICALMCPDLIIAVEKEK"
     gene            complement(26096..26758)
                     /locus_tag="P5549_00520"
     CDS             complement(26096..26758)
                     /locus_tag="P5549_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11588.1"
                     /translation="MATVTIFAGGFGSGKSELAINYALDISHFADNVILADLDLVNPY
                     FASRNVKDMLEARGIKLLAPAGELSLGDVPSVPPEIIGFLREDVEIVIDVAGDEVGAM
                     VLGYLSRYIKDSDYRFYLVLNPYRPFAGNLDLVMELRVLLENAANLTFTGLISNPNLM
                     AETNPDLIRNGHHIVTAYAQAMQLPVSWLAAEDQFYDDLVHDFGKDLQPITRYLRPAW
                     LQ"
     gene            complement(26927..27355)
                     /locus_tag="P5549_00525"
     CDS             complement(26927..27355)
                     /locus_tag="P5549_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11589.1"
                     /translation="MAIKIKLRFDYPGRGKSGKVFGKKSIEQIAEETRQQKVALLRNV
                     PSQGIRIEDIDMSSEVYTVFDEVNRKEVAYAPVFITISADHLEDALKFSMKEGFCTVT
                     VLEPEDVTLSKAEVERILYKANEELQDYKEYLLKRINNLN"
     gene            complement(27327..28103)
                     /locus_tag="P5549_00530"
     CDS             complement(27327..28103)
                     /locus_tag="P5549_00530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRW11590.1"
                     /translation="MRLAKYLAQAGIASRRHAEQMIKNGEIKVNGQQVTAMGIQVEPG
                     RDRVEYRGQMVNNCLPRLVYVLLNKPAGFICSVEDPQGRPTVLNLLGDMEARIYPVGR
                     LDYDTSGLLLLSNDGEFANLIMHPRYKMIKTYEALVKGQVSALKLQKMRQGLLLEDGL
                     TAPAQVKLLYEGGDCLLEISIHEGRKRQIKRMCAAIGHPVISLKRTGLAFLSLHGVEE
                     GHYRLLTDQEVVALKDCARGTDGYEREGDETDGHQDQTEV"
     gene            28344..28919
                     /gene="folE"
                     /locus_tag="P5549_00535"
     CDS             28344..28919
                     /gene="folE"
                     /locus_tag="P5549_00535"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRW11591.1"
                     /translation="MDKTFTVDKERIEKAIKEVLLAIGENPEREGVKNTPARVARMYE
                     ELFWGMNTDPRDFLDVSFTEYHDELVLVKDIPIYSMCEHHFLPFYGVAHIAYIPKGGK
                     VVGISKLARVAEAYAHRPQLQERLTSQIADCIFETLNPMGVGVVIQAEHMCMTMRGVK
                     KPGSLTVTSAVRGIFETRSQTRAELFSLILK"
     gene            complement(29118..30785)
                     /locus_tag="P5549_00540"
     CDS             complement(29118..30785)
                     /locus_tag="P5549_00540"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRW11592.1"
                     /translation="MKSDIEIAQQAIMQPITEIARYIGLNDDDIELYGKYKAKVTLET
                     WEKIKDRPNGKLVLVTAINPTPAGEGKTTTTVGLGMALNRLGQKAIIALREPSLGPSF
                     GVKGGAAGGGYAQVLPMEDINLHFTGDIHAVTTAHNLLAALLDNHIHQGNRLNIDPRQ
                     IVLRRAMDLNERALRNIVLGLGGKADGIPRESGFDISVASEVMAELCLASDLMDLKDR
                     FKRMVVAYTYDGQPVTANDLHAAGSMALLMKDAIKPNLVQTVENTPAFVHGGPFANIA
                     HGCNSVMATQMGLKLGDYLITEAGFGADLGAEKFLDLKCRFAGLKPDALVLVATVRAL
                     KNHGGVKKDKLAEVNMEALAKGVSNLEKQLENLNQYGPPVVVALNEFPGDTPEEIEFV
                     REKCRALGAEMAVSRVWAEGGAGGEELARKVITAAETPSTFRFLYELEMPVKAKIETI
                     CKQIYGADGVQYNKEADNMIRRFEELGYGNLPICMAKTQYSLSDDANALGRPTGFTVT
                     VREVRLAAGAGFIVALAGAIMTMPGLGKTPAAEKIDILPSGEIVGLF"
     gene            complement(30892..31425)
                     /locus_tag="P5549_00545"
     CDS             complement(30892..31425)
                     /locus_tag="P5549_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HRW11593.1"
                     /translation="MLAILDTVSRWAIPFLLLTIPIYGFLKRLPVYETFVEGAEEGFY
                     TAVKIIPFLVGMLVAIAVFRASGAMDYVISAIVPLMQKIGAPAEVMPLAVMRPLSGSG
                     ALGLATELIRQYGPDSLIGRMASVMQGTTDTTFFVLTVYFGSVGIKRYRYALLTGLSA
                     DITGLVASIYICHHLFS"
     gene            complement(31431..32018)
                     /locus_tag="P5549_00550"
     CDS             complement(31431..32018)
                     /locus_tag="P5549_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRW11594.1"
                     /translation="MLNVIWLLLLASGIIAAACNGNIEVVSQAALGAARDAVEVCFNL
                     IGIMALWLGIMKIAEKGGLIRILAYLLNPLLKWLFPSIPAGHPAMGAIVLNLSANMLG
                     LGNAATPFGMKAMQEMQQLNGGSDQASPAMCTFLALNTSCITLIPATIIGIRVSYGSA
                     NPTEIVGACIFATCCASVVAVTLDWLFRNLSHSGR"
     gene            complement(32154..32582)
                     /gene="ytfJ"
                     /locus_tag="P5549_00555"
     CDS             complement(32154..32582)
                     /gene="ytfJ"
                     /locus_tag="P5549_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HRW11595.1"
                     /translation="MESQQNFGNSRPIEGLMKTAMESIKEMVDVNTVVGDAVETNDGT
                     VIIPVSQVACGFAAGGGEYEFDNSNKSQEIPFGGGSGAGVSVKPVGFLVVRMNDVRLL
                     SVNGNTLAERVVDLAPQLIDKLEGLMERRNRHEAEGTAAH"
     gene            complement(32542..33201)
                     /locus_tag="P5549_00560"
     CDS             complement(32542..33201)
                     /locus_tag="P5549_00560"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HRW11596.1"
                     /translation="MKFHIVFNFLAVALVLSLYITFRIEIMVTGNERFQGIQIRFGLP
                     FYRFEQIYDYTDPRLHFLESLLMDRWQKSFKTQQTSSWKQFLKKLLRPRRLHLLSVWE
                     QQMGVVLKKILTLTVMEKFSWESRVGGKDAMRAAIYTGLLWMIKGWGMNWLTRHSHLK
                     KVQLVVEPDYLSTDFASQIDCILKIRIVHIITITIYLTVWKVRWWMDGIAAKLRQQPS
                     Y"
     gene            complement(33258..33815)
                     /locus_tag="P5549_00565"
     CDS             complement(33258..33815)
                     /locus_tag="P5549_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HRW11597.1"
                     /translation="MGNKDLEEEIMQKAQQNRKLARYMSSTRDLVEEQIIKARQRGDF
                     DNLAGAGKPLNLEENPFEPAEMRMANKILKDNDFAPYWIELGKEIDAAQDKLCREIDY
                     FKRYCQVFWTDQHDERAQQRFEYKKELFFMERKADLEKIDKKILDYNLHCPTFRQGRS
                     NLIIAREMEQIVREIETTIAKAKKP"
     gene            complement(33874..34395)
                     /gene="scpB"
                     /locus_tag="P5549_00570"
     CDS             complement(33874..34395)
                     /gene="scpB"
                     /locus_tag="P5549_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRW11598.1"
                     /translation="MLMREDIKAAIEALLFVSGERLETAELLEMLQIEEADLRSIMQE
                     LILEYHQSPSRGLQIISLEGGYIMATKPEFAGILSKLVKPARLRLSAAALETLAVIAY
                     RQPVTRAEIEQIRGVKVERVLSNLLEKGIIREDGHKEAAGKPILYRTSYEFMKIFGLS
                     SLEELPNPENELP"
     gene            complement(34389..35105)
                     /locus_tag="P5549_00575"
     CDS             complement(34389..35105)
                     /locus_tag="P5549_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRW11599.1"
                     /translation="MDYVVNLEHFYGPLDLLLYLVEKNEMNIYDIPIAIITDQYMHFV
                     TGSGHMELDQIGEFLMMASYLLSLKARMLLPGKPETTEDEEADPREELVSRLIAYKRY
                     KKAAEWLALRLDENYPRPFFRHGQLDMIEPAKEIVASVQTLLRVYLRLQEDELETAGY
                     CVPQGDINVDAKMEEIMQCLQKVKQTSFHNMFVGYQQRREILAGFLAILELIRLNRIE
                     AVQESRFGDILLRLQESAPC"
     gene            complement(35121..35753)
                     /locus_tag="P5549_00580"
     CDS             complement(35121..35753)
                     /locus_tag="P5549_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRW11600.1"
                     /translation="MQFIYNMLITLPAILIALTFHEYAHGRVADWLGDDTPYYQGRLT
                     INPLPHIDWIGFFALLLLHFGWAKPVQVNPYNFKNVSIKTGMMLVSFAGPAMNLLLAF
                     AGMILLRLIGFDPQTGLYGGALGAVIQMLIPLIQINIILAVFNLLPVPPLDGSKILAG
                     ILPDKGANFIYSLEQFGPLLLLLIIFTDLAGKIIWPLYNMIYTIIYGTVF"
     gene            complement(35737..38382)
                     /locus_tag="P5549_00585"
     CDS             complement(35737..38382)
                     /locus_tag="P5549_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRW11601.1"
                     /translation="MTLEIITSHNALDFDGLACMAAAAKLYPGAVRVFSGTVNKSVKR
                     FMSLYKDLLAIKTAKEIDVKAVQRLVLVDTASPHRLAQLSGVLNNPGLDIHIYDHHPQ
                     SEDDMRGSIVEIHPTGAATTILVEKIMERNISISPFDATIFSLGIYDDTGSLLFPSTT
                     SRDARAVAYLLSCGANLAVVSSFMEQPFSEEQRQTLHKLIEAGTHYKIKDWDIVTAIF
                     SGTEFVPGLDLVTYRLFEMENCDAAFVIAKMEGKVHVIGRSRSSQVKVNDVLKPLGGR
                     GHDQAASALVRSQECAAVEAIILDNLKLLIHPSITAADIMSSPVKTLRSTISMEEAGR
                     LMLRYGHTGMPVVENGKMIGMISRRDVDKARIHLLGHAPVKGFMTADVLSVTASTPVS
                     DIQKLMVEHDIGRLPVIEEDRLLGIVSRTDILRTLHGDDYPEDHELLYSYSEGSWENC
                     SEQMHRQLPQQLLNFLRTAGEIAAGLDYTVYCVGGFVRDLLLQVSNFDVDLVVEGDGL
                     RLAAALADTFKAKLRTHERFGTAAVILPDGSKIDVATARTEYYEFPAALPQVEKSSLK
                     EDMYRRDFTINTLAICLNPGHFGELIDYFGGSNDLRQGLIRILYNFSFVEDPTRIIRA
                     IRFEQRYQFKIETDTMRFARDAIERRLLGKLSHKRILSELILILNEKDPLPALRRMQE
                     IGVWKYVLPEINFEKVSWSKFKRVPLVTGWWLDRYYGWRFRTWLIYLLILFAQVNAES
                     REEILQNYPIDRTAKKAIIDAAAIPQLAARISENRLLVPSDFDRMLSGLDNENKLYLL
                     LCIKDEGAWDRINQYLDQKERVTIAINGQDLRDLGLKPGPAFKLILERLYEYKLDAKL
                     MSRQEEIEMVKQWLEEGSIPNAIHI"
     gene            complement(38544..38855)
                     /locus_tag="P5549_00590"
     CDS             complement(38544..38855)
                     /locus_tag="P5549_00590"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRW11602.1"
                     /translation="MPAYLVGALVFMICIVVFVLQNNTPVTIQFISWRTAEVSLALVA
                     LIAALGGAIITFLIDSFRAFKTGRKINELTHYNKKLEKEIIALRAKMTDAPAGNKPNI
                     P"
     gene            39133..40083
                     /locus_tag="P5549_00595"
     CDS             39133..40083
                     /locus_tag="P5549_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRW11603.1"
                     /translation="MGAIKTVRTLWADEKQRVAFISILSNTTLVLLKLMAGILTSSVS
                     IISEAIHSGLDLLAAIIAFIAVRISSTPPDDKHHYGHGKFENMSGTIEALLIFVAAIW
                     IIVEAYEKIIHGAQVVTIGIGIAVMAFASLVNWLVSGLLMRTARKTDSIALEADALHL
                     RTDVYTSLGVMLGLLVIAITDIQILDPIIAIAVALLICKAAYDLTVKALIPLLDVALP
                     PEEEAIIKEAIDSVSLDSLVNFHQLRTRKAGAERYVDMHLVFPRNLSFAAVHDMCDRI
                     ENEIELRLNHVHVLVHAEPCEDADCELCRKKECDIKADGD"
     gene            complement(40207..41055)
                     /gene="secF"
                     /locus_tag="P5549_00600"
     CDS             complement(40207..41055)
                     /gene="secF"
                     /locus_tag="P5549_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRW11604.1"
                     /translation="MQFIERRKWFYIFSSILIVAGIVSLFMQGLNWSIDFTGGSLLRY
                     KMDSSISADQVRNAVNEFNLVKEVSVQKSGDEFYLRTNELDQEQTAKMTAGLKEKFTN
                     VTLESAESVGATIGSEIARNALIAIGIALVLMLLYITFRFEFAFGLAAVLSLAQVVLV
                     VVGLFSIFQWEVSSYFVAAILTIIGYSINDTIVIFDRVRENLRTRKKEELHTLLNRSI
                     MQTMNRSVNTVLTVLMPLVTLLLFGGSTIKVFVIALLVGFIYGMYSSICVACPLFYDI
                     KQRSAS"
     gene            complement(41068..42273)
                     /gene="secD"
                     /locus_tag="P5549_00605"
     CDS             complement(41068..42273)
                     /gene="secD"
                     /locus_tag="P5549_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRW11605.1"
                     /translation="MQKNTSIAIIGAIIVALAFLAYFTYQPIMNNIKLGLDLQGGLHV
                     VLQAQEKEGKKVSADTIQKSIGVLEERVNSLGVSEPSIYPQGNDRIVIELAGVDDPEA
                     AVDIIKNTAQLEFWDETGKVLVTGENLKDAQAQLSSSGGEAMVNLEFDKTGAKLFAEA
                     TAANVGKPIAIVLDGKTISAPTVNEPIPNGQAQISGNFTAKEAQDLAVLLRSGALPVS
                     FSIMEKRTVGPSLGSDSLDFSIKAGLMGLLAILLFMLGYYRLPGLVADFSMVLYSVLV
                     LGIMALLGVVLTLPGIAAFVLSIGMAVDANIIIYEHIKEELRLGKTLKAAIESGFVRA
                     FWTIFDANLTTLIAAMVLIWFGTGSIKGFAVTLSIGIVASMLVALTFTRYMLVWFADI
                     TKNNKLYGV"
     gene            complement(42343..43002)
                     /locus_tag="P5549_00610"
     CDS             complement(42343..43002)
                     /locus_tag="P5549_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRW11606.1"
                     /translation="MVTLEDIQKSPVINTFIRKGNEHLGAMGYTDHGKLHITLVSRLS
                     RSILLQLGYDARTADLAGIAGYMHDLGNVVNRNGHSQSGALMALEILQAMRMHPDEIA
                     IIIAAIGNHDEGSGLPVNPVAAALILADKSHVHRNRVRNIDVATFDIHDRVNYAVEHS
                     FLLIEAEKNLISMELTIDTGICPVMEYFEIFLSRMLMCRRAANYLGCEFALIINGAKL
                     L"
     gene            complement(43005..43292)
                     /gene="yajC"
                     /locus_tag="P5549_00615"
     CDS             complement(43005..43292)
                     /gene="yajC"
                     /locus_tag="P5549_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRW11607.1"
                     /translation="MAAAATNTQQGWLTVAIYFGVFLLIFYLFIIMPRKKQEKKHDSI
                     LESLKRGDKIVTIGGIRGEISKVKEDSIVVKVSDNTEIEFVKKAISHKIED"
     gene            complement(43371..44477)
                     /gene="tgt"
                     /locus_tag="P5549_00620"
     CDS             complement(43371..44477)
                     /gene="tgt"
                     /locus_tag="P5549_00620"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRW11608.1"
                     /translation="MLDFQIIRRDSGSAARTGQLTTSHGVFATPIFMPVGTQATVKAM
                     TPEMLTEVGAGIVLSNTYHLYLRPGAGLVEKAGGLHRFMNWPRGILTDSGGFQVFSLK
                     GLRRISDEGVLFHSHIDGSRHFLSPEIVMDIEQKLGADIAMCFDECAPFPCSYEEAAQ
                     AVDRTTLWARRCRENHSRSDQALFGIVQGNIFADLREKSAAEIVALDFPGYAIGGLSV
                     GEPKADMYRILDLTEDLLPKNKPRYLMGVGAPEDLLEGVKRGVDMFDCVLPTRIARHG
                     TAFSRRGKVVVRNATYAEDFTPLDPNCNCQVCKNYSRAYLRHLVKAEEILAHILLSYH
                     NIYFLLKLMQDIREAIDSNTFNLFYQQFYENYKL"
     gene            complement(44498..45553)
                     /gene="queA"
                     /locus_tag="P5549_00625"
     CDS             complement(44498..45553)
                     /gene="queA"
                     /locus_tag="P5549_00625"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRW11609.1"
                     /translation="MNRQDLSPLYNLQRYMFDLPPYLIAQYPVEPRDSARLLVMDRTA
                     GKLEDRLFTDIIDFFSPGDTLVINQTKVIPARLFACKSTGARIEILLLNQQGEYWEAL
                     VKPARRMPMGSRVYFPDHSGVEIEVTAVRENGGRLLRFLNCPDVMAFLDQAGSMPLPP
                     YINRSATEEDKTTYQTVYAQAEGSAAAPTAGLHFTEELLKQLRDMGVNIAQVLLHVGL
                     GTFRPVSCADIREHQMHYEYFEIDEDTAQLLNNTRRNGKNIVAVGTTVVRTLETVYNK
                     DYGFGGQSGRTNKFIYPGYQYQAVDRIITNFHLPGSSLIMLVAAFAGLEPTLQAYRHA
                     VQEGYRFFSYGDAMLII"
     gene            complement(45550..46089)
                     /locus_tag="P5549_00630"
     CDS             complement(45550..46089)
                     /locus_tag="P5549_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRW11610.1"
                     /translation="MAKILLHICCGPCASYPVPALREEGFEVMGYYFNPNIHPYTEWQ
                     KRRDSLQSYAEEVNLPMLFAEDYDPAQYFQTISFRESRRCFYCYQLRLEKAAQIARKG
                     KFDSFTTTLLVSKYQRHDWIQELGQLIGEKYEVPFLYRDFREGFRQTGINAREMGLYR
                     QQYCGCLYSEMERYRRKTK"
     gene            complement(46134..47177)
                     /gene="ruvB"
                     /locus_tag="P5549_00635"
     CDS             complement(46134..47177)
                     /gene="ruvB"
                     /locus_tag="P5549_00635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRW11611.1"
                     /translation="MSDRRLISSQLNEEDLQEGSLRPLSMADYVGQERAKQNLEVFIA
                     AARERRESLDHVLLSGPPGLGKTTLATIMANEMGQPIRKTSGPAIERPGDLAAILTNL
                     EPGEVLFIDEIHRLHRSIEEIMYPAMEDYTIDIVIGKGPAARTLQIDLPPFTLIGATT
                     RPGLLSSPLRDRFGISCRLDFYNQHELATIITRAASILGIAITADGAAEIARCSRGTP
                     RVANRLLKRVRDYALVKAQGNIDQTTASQALQMLEIDEHGLDTVDRMILDAIIIKFGG
                     GPVGLETLAAAVSEEGDTLTDVYEPYLLKLGLIHKTPRGRVATESAYRVMGYPMQKGF
                     QAASLFEDADRDD"
     gene            complement(47192..47794)
                     /gene="ruvA"
                     /locus_tag="P5549_00640"
     CDS             complement(47192..47794)
                     /gene="ruvA"
                     /locus_tag="P5549_00640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRW11612.1"
                     /translation="MIAFLKGTLFQMMSEAVVIETGGLGYEVQIHGRMAAQLPAPGGQ
                     VMVHTFLQVMENEFKLYGFASRGELELFKLLMGVSGIGARAAMNLLGKLDGEQLCSAI
                     ISGDEKVLVGVPGIGKKTAQRLIFELRDKLGNYQGTVSLGGENANAAEEILQAMESLG
                     YSRSEIYPILTNIQSSGELSPRVEDNIKKILKRQALLMKR"
     gene            complement(47791..48291)
                     /gene="ruvC"
                     /locus_tag="P5549_00645"
     CDS             complement(47791..48291)
                     /gene="ruvC"
                     /locus_tag="P5549_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRW11613.1"
                     /translation="MLVLGLDPGTATTGYGLVEYVRSREKMIAYGTIKTSAGIAMELR
                     LLKIFQELNQILDTYRPEAVAIEQLFHHKNAKTVITVAQSRGVLLMAAAQRGLELAEY
                     TPLQVKQAVCGYGNAEKKQVQMMVQNILKLEELPKPDDAADALAIAICHLHSRRMGRI
                     KNGVGR"
     gene            complement(48521..49087)
                     /locus_tag="P5549_00650"
     CDS             complement(48521..49087)
                     /locus_tag="P5549_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11614.1"
                     /translation="MGKTKLGIILILTLLASSVAGYTLSGLIRNQQKWPIAQKPTVQT
                     AGWERRVQGDTAVVWEKEYVRSQKMQISEFPEREEILGKTLTDVKKIYPADQGFQVSW
                     DGKTLLIHQTIDDWMAEDKQKLRFKVFQDRVSVYQGPDSEHDTLLKVTGIVFARLPEQ
                     IRQDIMKGRYEFNNESSLNDALENLDEY"
     gene            49316..50041
                     /locus_tag="P5549_00655"
     CDS             49316..50041
                     /locus_tag="P5549_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11615.1"
                     /translation="MTNIDARVETICSELNHLSHGLMDIYQSYNDAPAYQSTFAPEKF
                     AVNQENLDEFDWSGMQYDFNVDLFETAPKEINWDDVNASIDEAIASGHLIAPAAIDYA
                     PKRSYGHNVLRSAGTYYKGDIRDMDFMLRQLKRLEANPADIFSLDDSAFDRSYNMVSN
                     FEMKVPLEIYGQGEELVIKADIPEILFNSAKAEDLNTLPFKSVLALLPERINTRQLKP
                     TFVDGEFRLELPKREEKRVRKVF"
     gene            complement(50174..50926)
                     /locus_tag="P5549_00660"
     CDS             complement(50174..50926)
                     /locus_tag="P5549_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRW11616.1"
                     /translation="MSGHNKWSTIKNKKARTDEKKGKEYTRIAKEIILAARSGGGDPE
                     ANSKLRLAIQKAKAVNMPNDNISRAIKRGTGEIESDAIEEIKYEGYTAGGAAILLDIA
                     TDNRNRTASDIRHLFAKYGGNLGESGCVAYLFDRVGLIVINREDIKTDEEEFMLMAIE
                     YGADDVREADDTLEIITSPDAFMEVRDRLEKEIRIEDADIVMLPQTTVEIADEATAQK
                     ILKLVDTLEDHDDVQNLYSNFSIADELMEKLM"
     gene            complement(50955..51626)
                     /locus_tag="P5549_00665"
     CDS             complement(50955..51626)
                     /locus_tag="P5549_00665"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRW11617.1"
                     /translation="MNSRQRNVLIRIISALAIFAYVGYLAWPVLPHFDFTVMVGFVVL
                     YLVWQLISEIWIYQDPGDIVIEDDDQKSYLYLQASFMLALLFATIDFVEWHVTRRFDW
                     EPTIIYVGLVLFVVSCLVRWWGFRSIGRYFNARIAVYSKHQLITEGAYRRIRHPLYLG
                     SLLSFLAIPMIFNSWGALLIIVLTTIPALIRRVNLEEEFMLHHFGDEYQAYMRTTQKM
                     IPGIW"
     gene            complement(51628..52530)
                     /gene="nadE"
                     /locus_tag="P5549_00670"
     CDS             complement(51628..52530)
                     /gene="nadE"
                     /locus_tag="P5549_00670"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRW11618.1"
                     /translation="MLTSLDKGLALQARPDWKKAEQEYWTISTAMTVQQTIPRPIQSF
                     NKEESTMNTEAVSDYLVNWLREKVQAAGCRGLILGISGGIDSAVAAALAKQAFPDDCL
                     GLLMPCESSAEDLQDGLLLVESLDMHYHIINLEEAYRALLKELAHAIDLPEDSGRLVR
                     ANVKPRLRMTSLYYMAQAHQYLVMGTSNKSEITVGYATKYGDNGVDLQVLGDLRKEQV
                     YALARYLNIPEQLITKAPSAGLWGGQTDEGEMGFTYRELDEYIRTGAGATELVGKIQT
                     MYERSAHKRSVPPIAVLPAGMGEA"
     gene            complement(52666..53424)
                     /locus_tag="P5549_00675"
     CDS             complement(52666..53424)
                     /locus_tag="P5549_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HRW11619.1"
                     /translation="MDRKTGEQYVQRYYRALHEARDLRYFHIQVKETDLAVAIDQVHD
                     TEHLIALCVKEVKRLRAELEGYLSRQPAFRTSFVPLALLPDAPEIARRMAEAAVQAGV
                     GPMAAVAGAFAQAVGERLMREVKETIVENGGDIYLNSQRDRIVAVFAGPSRFSQRIGL
                     KIKADSTPLGICTSSGTVGPSVSLGKADAVVILAPDTALADAVATGAANLVQDEEDLL
                     KAIKYAQNIKGVQGILAIKGEKMAAWGELEIVRL"
     gene            complement(53433..53855)
                     /locus_tag="P5549_00680"
     CDS             complement(53433..53855)
                     /locus_tag="P5549_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HRW11620.1"
                     /translation="MKNKLVCYFSTAQTEQPIIYRLVKNYDLIINILKADINPQKEGY
                     LVVELEGSKENYEAGTRFLESLGVVLEPLSESIIWNEQICIQCGACASFCPTEALAID
                     RDTMEVSFHNERCVVCGMCLECCPTRAIILHFEIKEAV"
     gene            complement(53855..55084)
                     /locus_tag="P5549_00685"
     CDS             complement(53855..55084)
                     /locus_tag="P5549_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HRW11621.1"
                     /translation="MAVQKSYEEINEKIKKGEAVVVTAEEVIGIVEEKGYEQAAREID
                     VVTTGTFGPMCSSGAFINFGHSNPRIKMKKVWLNGVEAYSGIAAVDAYIGAAQVAEND
                     PENKVFPGRFVYGGGHVIHDLVAGKEIRLEATAYGTDCYPNRKVDTVITLDDVNDAFL
                     FNPRNAYQNYNCAVNLGDRTLYTYMGILKPRVGNASYSTAGQLSPLLNDPHYRTIGIG
                     TRIFLGGGVGYVAWNGTQHHPNVLRGDNGVPKVTAGTLSVIGDLKGMDPDWLVGLSYI
                     GYGATMAVGIGIPIPILNEEMLHYTAVKDEDIYCPIVDYNEGYPYSKPMDLGFANFKE
                     LKSGRITINGKSVVTTPQSSYPRARKIAEILKQWILQGKFEITRPVAPIPSAEADIVF
                     KSIPERTPNGLANGRNK"
     gene            complement(55105..57615)
                     /locus_tag="P5549_00690"
     CDS             complement(55105..57615)
                     /locus_tag="P5549_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRW11622.1"
                     /translation="MDLQSLLIERVVILDGAMGTLLQSRGMQPGDCPELFGAEHPEIL
                     ADIHRQYLEAGADIIETNTFGGSRYKLAEYGLENRVAEINTELVSIARRAVGQRGLVA
                     ASIGPTGKLLRPMGEVEFDDLYAVFKEQVLACAHAGADLISIETMTDIGEMRAALIAA
                     RENTRLPVIAHMSFEADERTMTGTDPLTAVVILEALQPLAIGANCSGGARELLPVIQR
                     LARYGSTWLSVEPNAGLPRLVDGRTVFPDTPEEMAEYALRLKEAGANLIGGCCGTTPN
                     HIQSMARVLRGMKPAARSQAAATALASRSRCVLLGDKHPLAFIGERINPTARKKLAQD
                     IKDGQMQVVVAEARQQQEAGAPVLDVNMGVPGIDEVGAMQKAVSLIQAALDIPISLDS
                     TDPEVVEAGLKMFVGRPLINSTTGEDKNLEKILPLARKYGAAVLGLCIGEQGIPDRAE
                     DRVAVARKILQRSLEYGLRPQDVLIDTLVKTASAQQDQVMETIKALQMIKEQLGLATV
                     LGVSNVSHGLPAREILNSAYLAMAWSAGLDLPILNPFDERMMDTCRAAAVLLGKDVNS
                     AAYINKYKDYKAASATATTLRHAVCSSCNIPVLLQSGSEPAQASPTATLPAAPACEDN
                     TIQGRIRKAVLQGDKENITALIDEALLQSKLEPLEIVNQAMIPGIEAAGELYDQKRYF
                     LPQLMTAAETMKRAFAILQPLLSRDGGGHAGVVVLATVEGDIHDIGKNIVAVLLENYG
                     FKVIDLGKDVKADVILERAALENADIIGLSALMTTTMPRMQEVIAGVQARGLKSRVMV
                     GGAVLNQEYADKIGADAYSEDARAAVLTAQRLMGLLTK"
     regulatory      complement(57674..57773)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(57812..58183)
                     /locus_tag="P5549_00695"
     CDS             complement(57812..58183)
                     /locus_tag="P5549_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRW11623.1"
                     /translation="MLGNIGPWELVLILLIALIVVGPGKLPDVARSIGKGLNEFRKVT
                     TGVRKEFQDAVNLDDLTKPAPKDIPPNPLEAKPVEEEIIVASEVIADESDEPDATEVG
                     EKSAVLPEAEQDKIPDDKTAN"
     gene            58371..59903
                     /locus_tag="P5549_00700"
     CDS             58371..59903
                     /locus_tag="P5549_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRW11624.1"
                     /translation="MLATTNTLLINGIEAVPVKVEVDIQNGLPNFELIGLAQAALKEA
                     RERVKAALKNSGYEFPNRKIIVNLAPADLKKEGSHFDLAIALGILLASGQIEATFIEN
                     LFFAGELSLDGSLRQVPGILPMSLALIGATPDTRFIVPAANCREAGLVHETHTLAAAS
                     LQQVIDWLEGNDTLDLATEEYGKENRSGEPQPDFAEVKGQESAKRALQVAAAGLHNVL
                     LIGPPGGGKTMLARRMPSIMPDMTREEILETTKIYSVAGLLTADHPLIAVRPFRAPHK
                     NASTASIIGGGKVPRPGEISLAQNGVLFLDEFTEFHRDVLEALRQPLEDKLVTIARAQ
                     ATCTYPANFSLVASCNPCPCGFYGSDLECTCTPLQIQKYLGRISGPLLDRMDLHVEVP
                     RVIFEQLRTDSNSENSASLREKVLLAREIQLQRFARHKFKLNSQMRPTDVKKFCRLDE
                     ASEALLKNAFDRLKMSARAYDRILKVARTIADLDNVANIRIQHLAEAMQYRSLDRKYW
                     HN"
     gene            60128..60535
                     /locus_tag="P5549_00705"
     CDS             60128..60535
                     /locus_tag="P5549_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW11625.1"
                     /translation="MSYKKLGKRIQHAREEAGLSQEQLAAILGCSQPTLSNYEKGKSR
                     IYLTQLQKVSEILNRPVQYFLEAMEPSEEERGQAYAAEISRSYLEEASSQMDKDILSI
                     LKILQELSPEARRTVYEFAQWEYHKSRRYGNNG"
     gene            60528..61667
                     /locus_tag="P5549_00710"
     CDS             60528..61667
                     /locus_tag="P5549_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW11626.1"
                     /translation="MVNFALNNNQQSLIKSIRLLAPQLAQKEMEIDQHRSEAFDCSLV
                     NELAKLNLLNPMVPPEFGGRGLNYFDYALLMEEIGAVSPGLAAVMIFNCHFISILDSI
                     GTEEQKAQYLPVFTQQQPKLAAMALSEKNAGSDAGNMSTLAVKKNDHYLISGIKEYVT
                     NGTNADYIVCFATLDPTRARSQMLALLVPGNAPGIRQGQLRKTMGIRYAHCTELIFDN
                     VSVPAANLLGEEGNGYMIITQAIDRDKVLTGAIGVGIARAAFDMALAFAKDRKQFGRT
                     IYENQAIAFELSKMAAQIEAARMVVWKASWLLDNNEDFTVAASIAKITGTTTAEEVVS
                     KAMDIVGGRAYLEGHPLEKLYRDVKIFSNLEGTNHIQNAIITSLL"
     gene            61727..63226
                     /gene="glpK"
                     /locus_tag="P5549_00715"
     CDS             61727..63226
                     /gene="glpK"
                     /locus_tag="P5549_00715"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRW11627.1"
                     /translation="MDAYVLSIDQGTTGTTILIIDHEGRIVARAGREFKQYYPQPGWV
                     EHDPREILEITRMLIRLAISQAGLSAGQIKAIGITNQRETTVVWDQYSGQPIGNAIVW
                     QCRRTAPLCEEMKAAGWTEKIKARTGLIIDAYFSATKLAWMLENIPDARKKAEDGRLL
                     FGTIDSWLLWNLSGGRVHATDYTNASRTMLYDIHQLGWDEEILAYLNIPPTMLPAVRS
                     CSEVYGLCQADLLGEEIPLAGVAGDQQAALFGQHCFTPGSAKNTYGTGCFLLMNTGCQ
                     AVTSAHGLVTTIAWGLNQEITYALEGSVFVAGAAVQWLRDEMQLIANSAESEAVARSL
                     ESNDGVYMVPAFVGLGAPYWDMYARGTLVGLSRGTGRLHIVRAVLESIAYQSCDVLTA
                     MMTDANIRLDSLKVDGGACSNDFLMQFQADMLGVTVERPAMIESTALGAGYLAGLATG
                     FWSNLEELQQTRKVDRLFHPRMEAAQRQIMLNGWQKAVKCALTTPGKAE"
     gene            63305..64417
                     /locus_tag="P5549_00720"
     CDS             63305..64417
                     /locus_tag="P5549_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRW11628.1"
                     /translation="MYEDCVFLDFDYMEKFMAEVFCGLGVPREDAEICADVLITSDKR
                     GIDSHGIGRLKPIYYDRIKAGILDPVTRMEIIREGPTTAVIDGHNGMGHVIGQRAMQM
                     AIDKAKKFGMGMVAVRNSSHYGIAGYFALMAVQNGMIGITGTNARPSIAPTFGVENML
                     GTNPMTFGIPTDEPFPFVLDCATSVSQRGKIEVYDRENKEIPEGWVIDKSGQTRTDTH
                     QILLDLVSGQAALTPLGGIGEDNGGYKGYGYATVVEILSAALQGGTFMKALSGRDANG
                     QLRPYNLGHFFIAIDIEAFTELDSFQKSTGDILRALRASEKAPGASRIYTAGEKEYLA
                     WQERQGKGVPLNKVLQQEVLQMREELGLKDFHFPFE"
     gene            complement(64648..66612)
                     /locus_tag="P5549_00725"
     CDS             complement(64648..66612)
                     /locus_tag="P5549_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRW11629.1"
                     /translation="MNEQGYSFFGSRNLRLTSPQLQGTDVQILQALLNQLPDQIVPGK
                     LLVDGVFGPLTRTAVRNFQKYFGLVRDGIVGPKTFLRLGHRTGVYATGEKVFSSRTLK
                     NGSRGADVTILQNRLTAYKKAYLNRRADGRFGLYTEGAVQMLQADFPDLVSDGQAGPD
                     TCRKIFIHAPLGGRTLRSNDQGLDVYWLQYYLYQLNIYRGEINGYFQPATLAAVKDFQ
                     TAAAIVVDGIVGAQTYLALGASIAFPQQEYYYRVQAGDSVFTISRLFKQKSEAIILLN
                     HLVAPDYTIKTGQLLLIPPPLSFHLAEQGETLKKVAEKYDLPLGELQQANNLVPDDFL
                     IPAETIVLPGYNAKLQGEIAFLQPTDSSDDLIKLSLASGSATRLARFANLSRRELFLR
                     RDRKAVALLADDGHRIIIHDLTEGTSRSLNLSEPAESIAWSYDGSKLALDNGRIISTQ
                     DGRTLFSFSGMMPQWFTDNQSLLYSDGVSALRKISTETGNDQEVLALPDYSIWFFALA
                     AEINKVLIMAFADPGRVTFTFLYDLTNQDLIEISRNDFFGQWSRTRDYFLLLQRDYFG
                     EFLPWFYLKVNRYYREVALEGEEIYAKEVSLNNDNFSPADQAFLLVLSNPATFYPIPV
                     VHRDIYAKTIDARLLTQLTIEKNSYSPVWL"
     gene            complement(66703..67680)
                     /locus_tag="P5549_00730"
     CDS             complement(66703..67680)
                     /locus_tag="P5549_00730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRW11630.1"
                     /translation="MDYSDFTKEQLLERIEELEILNRQLLHEKEQETRLEYAWTGNLG
                     HWYWNIRTNAVTFNPLKITTLGYDPAEMPQPVPYQFFTERIHPEDLPLAMDVMRNHLY
                     GRAGVYEVEYRIQAKDGNYKWYYDRGRITQYDENGKPVFLAGIVFDITAKKAMQIELE
                     QKNKILAEQAEIDGLTQIGNHRTLIEHLKAKIVDANRLDKPLSVALFDIDNFKQVNDS
                     KGHIYGDKVLMKIAALIKESVRDSDFPGRYGGEEFMVIFPNADLTAAARVAERIRQAV
                     ESNLFDDGLTITISGGVQQYHGEELSELIHAADMKLYAAKKRGKNQIVS"
     gene            complement(67717..68583)
                     /locus_tag="P5549_00735"
     CDS             complement(67717..68583)
                     /locus_tag="P5549_00735"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRW11631.1"
                     /translation="MAEYIKNTGNFNGLENTRIFYQSWQAECPRGVVVIAHGIGEHGS
                     RFRNLIDHMQDDGISFYALDHRGHGRSEGKRGHINSFNDYTADLKTFCKLVRDKLPGL
                     PLILLGHSMGGTIACKFALDYPGIIDGLVLSSAGLIQAVKVPIWKKTMATLLANIIPG
                     LSMPTGLDSSALSHDLKVVDDYVHDPLVHDLVSARWYMEFTRAGQECLQRAGELTLPL
                     LIIHGSADAVVDPQGSRQVMQKASSQDKRLFLFDGLYHETMNELSPDREKVLETVRSW
                     VIQQVEGKTVAV"
     gene            68795..69289
                     /locus_tag="P5549_00740"
     CDS             68795..69289
                     /locus_tag="P5549_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRW11632.1"
                     /translation="MEPEFKTIAESVVSLSQIILPAQTNPAGTAHGGELLKMMDNAAG
                     ACATKHSRAIVVTAGIDNITFHAPVFMGNLTICSANLTYVSNRSMEIAVNIEAEDLLA
                     GHRQCCMTAYFIFVALDEKTMTPKAIPQLQLLNETERQEFEAGRLRTQTRKESPKACW
                     LPLY"
     gene            complement(69538..70353)
                     /locus_tag="P5549_00745"
     CDS             complement(69538..70353)
                     /locus_tag="P5549_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRW11633.1"
                     /translation="MLIYGHEIRAQLKQQLINRIGSRKIAMAVVQVGEQPDADAYING
                     IKRFGNELGVDVAVIKFSEDISEEKLLIELKQLNQDERYNGIMLQKPFPPQLRDEVIV
                     NAMNPDKDVEGIHNFNLGKIINRENGIRPATPKAVIRMLKDHNVDLEGARVTIVGRSS
                     ILGSPLALMMMAENATVTVCHTRTRDLAEEIKRADIVVAAMGQLNFIKAEMIRKETII
                     IDAGINFDAAGNMFGDVDEQARSKARMASAVPGGVGLVTVAELFDNLCLLQEI"
     gene            complement(70582..>70886)
                     /locus_tag="P5549_00750"
     CDS             complement(70582..>70886)
                     /locus_tag="P5549_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRW11634.1"
                     /translation="YLQQQGYEILTRNYRCRYGELDLICRKGGLLVFVEVKTRRSDLF
                     GSPEEAITRTKIEHIKKAALHYLGDQPAKHRETRFDVVTIMMRSSQPDINHIKAAF"
BASE COUNT        17470 a        19228 c        16235 g        17953 t
ORIGIN      
        1 ctggcatatt catcagcagc agtgtcctgt tttactaaaa taccgcggtc tgcgcccatg
       61 gcaagggctg tacggatggc atccatggct ttgtcagcac cggctgaaac aaccactacc
      121 tcagtagcaa ttttcttttc ttttaattgt attgcacctt caactgcaac ttcatcatag
      181 ggattaataa taaggttaat ccctgcatca ttgattttgc catcttttaa ctcgattttg
      241 gcttctgtgt caaaagtctg cttaacacat actagaacct tcaagttcat tcctcctttt
      301 catctgctgg ttatccgttt tttaagagta agatcactgc ccaaaagcct acagtaattc
      361 ttcaatcacc cccatgagta ttaatatata tttactgttg cctacggcaa agttaccgcc
      421 agagtttcta tgtttaccac cgacatttca ccggcttatg cagacagtgt ccgttttctc
      481 cactgctttc ataagcagaa tacggggaag ttccgatagc gcaaaaagcc atagacaaat
      541 aaatatcttc tatataaaag tccaatttcc tgcttgtgtc cagaaatcaa gcaaattttt
      601 attaacatat tacctctata ttgtaagata ctagattttt tgatagaaaa aagtgtccaa
      661 ggattgaaag cctgcttgtg ccggtcgaaa aatctgttcc gtactgccaa taaacaagac
      721 accgcctggc cgtaaagcct gggcaaagtt acggtaaagg tcctgtttgc tctcttccgt
      781 caggtaaata aacacattgc ggcacaaaat cagatcatga tccaattcaa acttgtcccg
      841 cagcaaatca tgacgggcaa actcaaccat tcttttgatt tcctcaataa cgcagtaatg
      901 atctccatct gattgaatat aacgatgcaa gtagccgggt ggcagcgttt tgaaggcttt
      961 accggtatac acggcttgaa ttgctttgcg cagcacttcc tgatccaaat cggtggcttt
     1021 tattttttgc acatgcccgg gaaattttgc atgaaaaagc atggccagtg aatacgcttc
     1081 ttccccggtt gaacaaccgg cactccacac cttcaatttg gttctatgtg tcaacagctg
     1141 cgggatgatt ctctcttcca acactttcca atgatccgga ttgcgaaaaa attctgaaac
     1201 atttatggtc aagtattcga taaatttttg gcggacaggc aagtttatct ctaaaaggct
     1261 gacaaaagat ttgtaatccg ggcactcgac cgttttcata aaactattaa tacgtctttc
     1321 catttgcggt cttttatatg caagcagatc gattcggctg atggtttcaa acttttttac
     1381 aaaccattcc caatcatata catccttctg caaaaatgcc cctcctattt catcatgttc
     1441 aaagcctttc ccatcgcttc cacagtcaat tctatatcag tcagatcatg ggctccggaa
     1501 atgaaggaaa cttcaaattg tgacggcggc agatacactc cgcaatgcag taaatttctg
     1561 tagaagtcgg catattgttc agtattgcaa cactgtactg attcatagtc gcgaacctct
     1621 tcttccgtaa aaaagacgca ccacatggag cccaatcgct tgattgaata aaatcggttg
     1681 tgttcttcaa atagcgttct gatttttccc gccagtacat agcccagtac ctccagacgg
     1741 tcatagacat ccccgtcttg caattccttc aacgtagcaa tgccagcagc catggccaga
     1801 ggattcccgg acagcgtacc ggcctggtag acttctccca gcggggctac caggtccatg
     1861 atttcctttc tgcccccgta tgctccaacc ggcagaccgc cgccgataat tttccccaac
     1921 gtggtcaagt cgggtgtaat gccggttaga ttctgtattc cgccataaca caggcggaaa
     1981 ccagtgatga cttcatcgaa gatcaagata ctatcataca tactggcaac ttcccgaacc
     2041 gtctgcagaa attccatttc ggctgggatt aaacccatat tgcctgccac cggctcaata
     2101 ataaccgcag caatatcagg cccgtactgc atgaaggctt ttttcaccga ctcaatgtca
     2161 ttgtatttgc acagtaaggt atgttcgcta atggagcgag gaataccagg actggattga
     2221 cttcctccgg taagcattcc ggaaccggct ttgagcaaaa acgtatcggc atgtccgtga
     2281 taacaacctt cgaatttgat gatcttgttg cgtttggtat aggcccgggc caggcgaatg
     2341 gcgctcatgg tggcctcggt gccggagttt accattctca ctttatcaat ggaagggaac
     2401 gcttcgcaga tcatctccgc cagttcgatt tcacgcgcac aagtagcacc aaaactgctt
     2461 ccgtcccggg ctgccgcgat catcgcttcg actacggcgg gatgactgtg tccaagaatc
     2521 agcggccccc atgaaccaac ataatcaaca aattcattgc cgtcctcatc gtagattctg
     2581 cagccgctgc ctcttttcat aatcagtggg tccattccca ccgattgaaa ggcacgtacc
     2641 gggctattta cgcccccggg catcactttg agcgcctgcc gatagatctg ttccgatctg
     2701 gtggttttca cttctttttc ccccattcgc gaccgatctt catcggatcg gtttcctcaa
     2761 tcaaaaaagc gcatacttac ttttttcagt tcccgaatac tgtttaagac aacataatca
     2821 tccaagccgg ttacatcagc aattcggctg atcgtttccg ctaggtccgt ctgactgcgg
     2881 gcatgcacca tggagaacaa attataacca aaatcagcgc tgacctcccg cagataacaa
     2941 tgacttacct catggaattc tgccatagct tcacctgccc ggtctgcttc aatcggaccg
     3001 accttccagg cgaccatggc attacaggta taacctgcct gctgatgcct gaggactgct
     3061 ccccagcgac gcacaacccc ttgtttttgc agatcttgaa tgcgctccag aacttcctct
     3121 tcgcttatct ccaaatcaac cgccagcttt tgaaagggac gtgattcaag cggtatatca
     3181 ccctggagaa attggataat ccggcggatt ttttcatctt catacatcat gattcagccc
     3241 ccatatcaaa cgaaaccttg attttgtaaa gcttgcgggc cggcatgggc aggatagaca
     3301 gtccgcaagc ctgttccaat gcctttaatt gctggatcag cgccgcctgc gaaggtgatg
     3361 tcagcgtaaa ccagaggttg taatgatggt cgcggacata gttgtgggtt actccggaca
     3421 gctggttaag tatggcggct acttcatcaa ttcgttcctc cggtacggtc atggcacaaa
     3481 gcgctgacgt atagcccagc tgtttggaat tcatgattcc gccaatacgg cgaatcaaac
     3541 cggtttcttt taaggcagcg atgcgggcca ggacctcttc ttcacttgtt ttcaatcggg
     3601 ccgccaagtc cccgaaaggc tggcaactga gcggcagagt gttttgcagg gcatccaaca
     3661 gagcacgatc aagctgatcc agtttcatat tttctgaccc tcttgccggg gattatagag
     3721 gcaccagctg tcttccgcca taatactgcc attataatag ttggcccgag cccggcatcc
     3781 cccgcacttt tcccggtaat cacaaattcc gcatttgcct ccatattcca tgctacgcag
     3841 ctcctgtaaa acagaatttt cagtccaaat tttatcaaaa ggaatttctt ttacgttgcc
     3901 cagtggcatg tccatatagg cacagggctg tacttctcct gtgggactta tgatgcaata
     3961 actgatgcct gccaggcatc cccttgagaa acgtatctgt atcccttttt tgtctgcaac
     4021 ccgaataaac tgtggtgcac aagtgggttt tatttcgatg ggcacttttt tttgtttttc
     4081 catcaaacgg gccagggttt tttcgtattc cgctactcgc agcgcttcct cttcaatatc
     4141 ggcaccacgc ccggtgggaa ccagaaagaa aacatgatgg gccatggccc caatctgtac
     4201 agcaaaatcg gtgatatctt ccaattccgc tacgttccag tccatcaccg tcgtatgaat
     4261 ctgaaaagga acaccggctt ctttgagatt taacatgcct tgcaccgtca aatcatacgc
     4321 atgatccagc ttgcgaaatg cgttgttttt ttgcggaatc aggctgtcga ggctgactcc
     4381 ggcagccata aatcctgcct gtttcagctt ttgggctacg tccggtgtaa tcagggagcc
     4441 gttggtgccc aatacaggcc gcagtccccg ccgggtggca tagcttccca attcatatat
     4501 gtccggtcgc atcaacggtt cccctccgct gaagatcata attttaaaac cggctttttt
     4561 aatctcatca atgagttttt gggcctggct tgtattgagt tcatcttcga gtcttgctcc
     4621 tgcatcgcgg taacaatgat cgcagaacat attgcactga ttggtagtat tccatgaaac
     4681 cagcatatca accctcctac acaatgtttc ctgataataa ccgggcagca tccttggccg
     4741 catacgttat gattaaatct gctccggccc tcttgatggc ggtgaggacc tccatggtca
     4801 cggcttcctc gttgatccag ccctgggcag cagcagcttt gatcaaacta tactcgccac
     4861 tgacattata agctgccgta ggcaggccaa atctgttttt cacctcatgc aaaacatcca
     4921 gataagccaa ggcaggcttt accataacaa tatccgcccc ctcctgaata tccagttcaa
     4981 cctcccgcat ggcttcccgg aagttgccag gatccatctg gtaactgcgg cgatctccaa
     5041 actggggagc acaatcggca gcatcccgaa acggtccgta gaaagcggat gcaaatttgg
     5101 ccgcataaga catgatgggg atgttctcaa agccgttctg atccagacaa ttccttatat
     5161 atccgactct cccgtccatc atgtcggacg gcgccaccat atcacaaccg gcctgcacat
     5221 gagaaagtgc ctcccgagcc aagaattcaa gggtacgatc gttgtcgaca ctccggtcgg
     5281 ctttcaagac tccgcaatgc ccgtggctgg tgtattcgca aaggcaaaca tcggttatta
     5341 ccattaattg cggacacaaa tccttgattc tttttatggc ctgctggaca gaaccgttgg
     5401 gatcacaggc ttcgctgccc gtttcatctt tgtgttccgg caatccgaac aaaagcaccg
     5461 ccgggattcc caaagattcg attgctttta tctcggcagg cagtctatcg gctgaaaatt
     5521 gaaaaactcc gggcatcgag tgaatcggat gtttttgctc ctgccccggt agaataaaca
     5581 acggataaat caagtcctgg gtcgtaagac tggtttctct taccatgaca cgcaacgttt
     5641 cattggcccg cagtcttctc attctttgta tcggataagt catccagatg cctccttgtc
     5701 gttcaggatc aatcatgctc cccgaatgag ggagactttc atgcaccgct caatcttttt
     5761 cagttaaatc agcaagaatt gatcgcaaca atccgtcaat ggtgtattca ctggcaacaa
     5821 tatcaaccgg caaccctttt tccctggctt tgtccgccgt aatcggaccg atacaggcga
     5881 ctttcaccct ttgtgccaac gctttggctt tttcgcttcc ggccatttct acaaaattgg
     5941 taaccgttga agaactggta aaagtaataa aatcaatggg ttgttccagt attgccttta
     6001 acttcccggg attggtgcag gtgggaacaa ctgcctgata aaggttgatt tcttccacct
     6061 ctgccccgcg tttgaccaac tcgagaggca aaacatcacg cgcccctttg gcccgcggca
     6121 ataacacctt ctgtcccgga acgattacat tctgcagacc atccaagatg ccttcggcca
     6181 aatactcagc cggcatcaga tccacccgca atccgtattg ctgcaagcgc tcggcggtcg
     6241 caggtccaat cgcacccact tttattccgg ccagactgcg gatatcctga gcgctttgaa
     6301 acaactcccg gaaaaaaatc tctaccgcat tgacactcgt aaatattatc caatgatagg
     6361 tttctatctg tgcaaaggca cggtgaagct gatcgaggtt ctcttcctca gtaatctcga
     6421 tcgtcggcaa ttctagcgct tctccgccca gttcacggat ctttttcacc aaaacactgg
     6481 cctgactgcg ggatcgagtc actacaatcc gtttccccca cagcggtccg gtttcccacc
     6541 aattgagccg ggagcgcaaa gcaacggtat cgccaacgac aatgatcgcc gggggcgtca
     6601 ggccagcttc cctgacccgg tcaacaattt cgtccaaccg tccgctgacc acttcctgat
     6661 cgggcatggt tccccgtcgg atcagtgcca ccggtgtttc cgggctgcgt ccgttggaca
     6721 aaaggttgct gacaatatat tcgaggtttt ccacccccat aagaaaaacc agggtaccaa
     6781 tcccggtgga tatctgttcc cagtcaatgg aactcacttc cttgccaggt ttttcatgac
     6841 cggtaatcac tgcaaaacta gaagtggcat cccgatgggt cactggaata ccggcataag
     6901 aaggcaccgc aacggccgaa gtaataccgg gcacaatcgc aaaatcataa ccccgctctt
     6961 tcacgtagag cgcttcctca ccgccccggc caaatacaaa aggatctcct ccctttaacc
     7021 ggaccaccat tttcccctgg gcggctttgt cggccaacaa ggcattgatt tcttcctggt
     7081 gaagcgtatg acggctggat tctttgccga cataaatcat ttctgcctgc ggattggccc
     7141 aggccaggat atccgaactg accaggcggt catagataac cacttccgcc tgttccagca
     7201 gttctttgcc ttttatcgta aacagcccgg gatcacccgg accggcacct accaaataaa
     7261 cgaatcctct tctagccatg ttcattctcc ctgccctctg atttcatcca gtacctgacc
     7321 ggccccccgg gacagcagtt gctcggccaa ctgtcggcca ttgccggcta catcatccag
     7381 cccgcattcc agggacgctt tatatacacg gctgccatcc agcgaggaaa tcaagccctg
     7441 aatatttaaa tgatcagctt cttttctggc caggcaggca attggcacct gacatccccc
     7501 ttccagtctc gccagaaaag ctcgctcggc ggtaatctcc aaccaggcaa cgggatcgtt
     7561 gatgggattg accatctgta aagtatcttc atcccctgcc cgcatttcaa ccgcaaccgc
     7621 tccttgccca accgctggca ggatatgttc cccggcaatg atttcgcgga tatggcctgc
     7681 aaaccccagt cgcttgactc cggcataagc caggataatt ccgtccaggc cttgctcctc
     7741 catctttctg atgcgggttt ccacattgcc ccgcatactg accagttgca catccggtcg
     7801 caaagttcgc aattgagcca cccgtcgcag actggaagtc ccgatggcag ctccggctgg
     7861 taaatcagcc aaacactgat aacgggtcga aagcaacaca tcatgcggat tttcacgggg
     7921 cagaaccgca cctaaacata agccctcacc caaaagcgaa ggcaggtctt tcatactgtg
     7981 cacggccata tcaattctgc cgtccagcag ttcattttca atttcatggg taaataaacc
     8041 cttgtctcca atttgtgcca gggggacatc cagtatttta tcccctttgg ttttaatgat
     8101 gacaatttcg acggatattc cttcccaacg acgggataat tcacgggcaa cgtattccgc
     8161 ctgccacaag gccagcttac tgccccgtgt tcccaacctt ataacccgca ttatacccct
     8221 ctttctcaca gtcaacttca agatcgaata aattcttaac gacttctgca tacaagtggc
     8281 cttgattgct tacggccatc tctttcattt tcagcaccgg cgagcgcaac aattgattaa
     8341 caatcgacgc agccatattc tgtataatga cctgatcacg ctcggaaatt gttcccatgc
     8401 ggttgaaggc ccttttcagc tcggcttgtt tgatcgtttc tccggtattc ttcagtgaag
     8461 caataaccgg tataacatac agtgaggcca tccactggtt gaactcttcc atctgccccg
     8521 taataatttc ctcggctgct tgtgccgctt cgaggcgctc ctgataactg acattcacaa
     8581 aatcctgcag atcatcaatg tcataaatgg tcaccccgtc aatggcctgt aaattggcat
     8641 ccacatcccg gggcacggca atatcgataa aaataattgg ccgcccctgc cgggctttaa
     8701 gcgtatttcc acagttgtcc tctgtgatta cacagtggtt ggctgccgta cagctaatca
     8761 cgatatccgc cctgctcatt tcctcggcaa ttttatcaaa aaatacggcc tttccttcaa
     8821 aaaaagctgc catttccatc gccttgtcat aggaacggtt ggagacgatg accgagtgta
     8881 tgccgtggga gatcagataa cgggtggtca gtttgccagt ctctcctgca cccaccacca
     8941 atacggtttt atcctgcaaa ctgcccaatt gctgaacggc caaatcgaca gccgcatagc
     9001 tgaccgagac aggatgctgg tcaattttgg tgtcagttct tacttttttg cctacataaa
     9061 gagctttttg aaacaaagca ttcaatactg cgtccgaagc accgtaatat cgggcggctt
     9121 cataagcttc cttgacctgt cccaaaattt gagtttctcc cagaatcatc gaatccagac
     9181 cagcactgac ccggaaaagg tgggcgatcg catcataaca attcggttga tacatatatt
     9241 cctgaaactg taattccgac aagccggaat aatcgagcag aaaatccttc agcaccttct
     9301 gcccggacgg aatatccctg gtcgtcgcat ataattcggt gcgattacat gtcgacaaaa
     9361 tgaccaggcc ttcgatttct tcgaattcag ttagccgctt gtaagcactt tccagctgtt
     9421 tttcagagaa ggcacatttt tctctaatct caaccggagc ggtgctgtga cttaatcctg
     9481 ccaagaggac atacattgat taattcgctc ctctgcctct tcatatcgtc cgtccagcaa
     9541 caaatccagt aaatcagaac caaccatctg ctccaatatc tgcttgcgct gatttatatc
     9601 cagggaggat tgtttaagtt cttctctgaa ttgacccaaa agattaacaa tatatccgta
     9661 ttcttctgtg acaatcgctt ccagttgccg gcgcagccgg gcagcaaaaa gcggactccg
     9721 gccttctgtc gagatggcaa gcgccaaaga gtcccgcctt aatactgaag gaacaaaaaa
     9781 atcacagttg ggggggtcat ccaccgcatt gaccaggatc cctctttgcc tgcacaaatc
     9841 ggcaatttcc ttgttcaaac tgttgtcatc ggtggcaata aaaaccataa acacgttgtc
     9901 cagatcatgg tcttcgaaag ctctgggaat ccattgcaag cgttcttgcc ctgcccattc
     9961 agcaatgccc tcttctgctg caggactgac aatttttatt ctggctccgt attcaagcaa
    10021 gctggcggct tttctttctg caaccgtacc gcctccaaca atgaggcagc ttttaccggc
    10081 tacatgcaaa taaataggat ataaatgctc catggtgtca taaaacaggt ccggaatcag
    10141 acctattctt tatccttttc gtctaattcg ttaaactcat cgtccttggc ttttctgaag
    10201 ctctgcagcg ccttccccat ggcttcgcca actttgggca atttgcccgg gcctacgata
    10261 atcaacacga tcaacagaat cagtccaagt tcccaaggtc caaaattacc gatcaatcca
    10321 aacaagacca aaacctcctc aaaaaactgt cgcctttatc aacaattcgt tatgcaccct
    10381 tgaaaaacct ttgtttgaaa acatctctct cgttattatg acaacaatac cgcactttgt
    10441 aaagaatccg cttccaggtc attaccccca aaaaagggat tctattctat ctttgatctg
    10501 ctccatttac cgaagaattt tattcatccg ttttttttgc cgcgtccttc tctgccgctg
    10561 tcggtttttt ttcaaaaaac cttcgcttgt tcttgcgctc ttcgctgggt cgggccagcc
    10621 gtgccaccag gacactgatt tcgtagagga tatatactgg tatgcccatc atcatctgcg
    10681 agatcggatc cggccccggg gtcagtgccg ccgccagaat gaagataatc aggatggcat
    10741 atttgcggtt gcgggacagc gtctgcgatg tcacaacccc cattttcgca agaaaaaaaa
    10801 cgatcaccgg gagctcgaaa accaatccaa aaggaattgt aaaagccgtt acgaaggaaa
    10861 catattgatc cactttaaac attgtatcca ggttggaacc agcgatatag ataaaaaatt
    10921 tcaagaccag cggcaggatt ccaaaataag caaagacgat accaccacag aacaataaca
    10981 atgtaaccgg aaacagaata taaacatatt ttctttcatg gggaaagagg gccggtttga
    11041 taaaactcca aattgccaga atcacaactg gaaaggctac gcaaaagccg gcgaaaaagg
    11101 ccagtttgag tttgacaaag aaggcttctg taaccccagt cacaatcagc ttttcattca
    11161 gattatgcaa aggggtgatg ataaatcgaa gaatctgctc attataggca aagcacacga
    11221 aggcagcgat gatgattgct attacgctga tgattaaaga cttccgcaat gcttccagat
    11281 gttctataat cgtctgcttg tcatctggac tcaaatctga aaaaaacaat ctgcaccctc
    11341 cttaataatc ccggccggag ataaagccgg ccatatcggc caaggcttga cacaccagcc
    11401 catcgggcag gaattgcagc tgtctgatcg ccagatctgc atatttttgg gttacctgat
    11461 aagcatattc aatcccttcg gaatgaacga caatatcgat tatctcttcc gcccgatggc
    11521 ggcagcattc cggtgaagat aaaagattgc ttaattcctg tccccgcggg tcaaacttga
    11581 tcgcatagat ggaaggcagc gtaatcaccc cctggcgtat atcactgccc accggtttgc
    11641 ccagcacctc ttccgaggcg gtaaagtcca gaatatcatc agttaattga aaagccatgc
    11701 ccagataata accgtaccgt ttcaaggcgg tgacttcccg cggcgcagcc tgcatgacca
    11761 ttgcccccag ctgacagctg actgagatta aaagggcggt tttgcgttca atgcggtgca
    11821 ggtagtcttt taagccctgg cgggtattat aacaactcag catctgctgg atttcacctt
    11881 cacaaatgcg catgctggca tccgccagca catccacaat atcgctgcgg tgatagctct
    11941 ccaccagcag cagggcccgg gcaaagacaa aattcccggc gtagatggac atgcggttgc
    12001 cccaggcgct tttcactgtt tcctgccccc gacgcaatga ggaattgtcg atcacatcat
    12061 cgtgcaccag ggtagccatg tgaatcagtt ccaaggcggt cgccaacgga atgactcttt
    12121 cgagcgagtt ggaaaacaat ttcgctgaca acaaggcaaa cgccgggcgg agtcttttcc
    12181 cccctgcttt gaccaaatga gcagaagcat tttccagcac cgacaaatca ctttggatat
    12241 tatttaataa ttgtttttca acctgctcca gttcctcggc aacatgttga aacaactcaa
    12301 atccagccat tatgttcccc ttaacacaaa gtaattatta ttataaattc aaaccaaaca
    12361 aatgccaagt aacagaacgg cttaaccata acgccgaaaa gaatcgttgg gaattcttaa
    12421 cgcatccaga atcttgccgg tcataaagtc gaccatgtct tcaatcgtct gaggctggtg
    12481 gtagaaagcc ggcatagccg gcacgattgc tacgcccatt tcagccagtg ccaacatatt
    12541 acgcagatga atggcactca ggggcgtttc ccgcggtacg atggtcagcg ggcggtgttc
    12601 cttgatcatg acgtcggcgg cacgttcaat gagattattg gatgagccat gggctacggc
    12661 tgccagggtg gacatggtgc agggcacgat gaccattccc tcagtgataa aggaaccgct
    12721 ggccaggcgc gcagctatat ctgcattatt atatagatac agattgggct ggcaaaaaaa
    12781 ggagtgcaag ctttgctccg gacgttcacc cagctcccag cccatttcct gttgaatcac
    12841 caaaaccccc ggctgactga ccaccaaatg aacctcataa cccagatcca acagggtttc
    12901 agtcaacctg attccataaa caacaccgct ggctccggtt atcgccacga tatatctcgg
    12961 catgcggttt cctcctgatc ccatcataat agtatatcca ttactgccgt aataaagatg
    13021 atcaacccca catagtgatt aatcttgaat gaagcaaagc taaccctttc caactgaccg
    13081 ggccgaacaa taacatgttc ataacataat atcaccgcag taaaaactgt tcctccataa
    13141 taccacatcc ccagatccag tatcatgccg gtgagaaaca ggcatacaat cgccaatcca
    13201 tgaaatacgg cggaaaaaat cagggcccct ttctctccga accgggcagg aatcgaatac
    13261 agatgattag cccggtcaaa ttcgatatcc tgacaggcat acatggtatc aaatcctgcc
    13321 gtccaaaaag cgactgccag ccccagcacg aaaggctgcc agtcaaatgt tccgctgatg
    13381 gcaaaccacc ctcctaccgg cccgatcccg atcgccaatc ccatgatgag gtggcaccac
    13441 caggtaaatc ttttggtata ggaataaatc cagagaacga aaaccgccag cggcgccagc
    13501 ttaaaacaca gcggattcaa atgatatgca gcataaaaca acaggccaaa aaaaacaaaa
    13561 accagcaccc ataccttttt gacggaaagc tggccggaag ccatgatcca atctgccgtg
    13621 cgcgggttgt gccggtcaat ctcccgatcg atcagccggt tcaaacacaa agccgcagat
    13681 cgggcactgg ccatggccaa ggtgatccag ccaagctgtg ccaggctggg cacgtccatc
    13741 agcgctaaaa aagcacccag ataggcaaag ggcaaaccga acaagctgtg ttcaatatca
    13801 atcatggtca agaacttgcg cagtttatcc aattccgtat tcactccact tgctggttac
    13861 cagcgccttg atttccgcac tcatgacgat atcttccggc cactctcgat tgtgaccttc
    13921 ctccggccat ttgcgggtag catcaatccc cattttcgag ccgaaaagcg gcagcggagc
    13981 agaatgatca agcacgtcca gcgggccctc aacaatcatg gtatcccgtc gagggtcggc
    14041 gttattgaaa accttccaca taacttcgga tacattctgt acgttaaccg tctcatccac
    14101 cactatgata tatttggcaa acatcatctg ccccataccc caaagagaat tcataatctt
    14161 gcgggcatgg ccgggaaacg attttttaat ggcgaccagc acgcaattat gaaacacacc
    14221 ctccatgggc agatgcatat ccacgatttc cggcagctgg aacttcaaca agggcagaaa
    14281 tattcgttct gtcgccaagg ccagatagca gtcctcctgc ggcggctgtc cgacgatggt
    14341 ggtgggataa actgcatttt ttcgccgggt gatacattta acatgaaaca ccggatactc
    14401 tccggtcagg gaatagtaac cggtatgatc gccaaaagga ccttccaggc gactttccgc
    14461 caaatcaacg tagccttcca gaacgatctc ggcatcagcc ggaacttcca tgtcgatggt
    14521 cttgcagcgc accagatcaa ccggtttttt tcgcaaaaag ccggcaaaga tcaattcgtc
    14581 gatatctgga ggcaggggcg cagtggctgc ataggcaatc gccggatccc cacccagggc
    14641 aacagccact tcaataatat cccggcccat ttccttggat gctctgtaat tggctgctcc
    14701 gtcatgatgc atatgccagt gcattccagt cgtttgcttg tcatacacct gtagacgata
    14761 catgcccaaa ttgcgcttgc cggtatgggg attgcgggta tagacctgcg gcagggtaat
    14821 aaaccgtccc ccatcctgcg gccagcattt taaaatcggc aactggtcga gatcaggatc
    14881 catggaaacc acttcctgac aaacgccact cttgaccatg cgcgggaaga acgatgacag
    14941 ttctgccaaa cgcggcagca atttaacttt gcccagcatg gtagtcggta cgttctctgc
    15001 gatttgcagc agttggttga tttcttcacc aatcgcatcc aaatcctcga cctgcaaact
    15061 catggccatc cgctttctgg ttccataagc gttgatcagc accggcaagt cagatccttt
    15121 gacattttcg aataataaag ccgggccgaa ggctttgctg acccggtcgg taatttcagt
    15181 gatttccagt tcgggatcga ctggagtctt gattctcacc agttctccgc gcgtttccaa
    15241 cacagatata aattcatgta aatctttata ggccattctc ttcctcctac aggattcatt
    15301 cttttctatg gtaacccggc tgccgttctc atttttcagg cctttcacca accatatcct
    15361 gcagccattt ctgtaaaaca aggtggttca ttccatacgc atgattgaat tgcttcagca
    15421 gcgccaggct tttttccagg aaagggcgag cttccagatc ggctccggca ttcagcagtt
    15481 ccatggccat tccgacttcc aatcccagac gtctgtacag tgcaagcgca tcctcatcca
    15541 gggctttcaa gcgtccggct gtcataaatg cggtttcgta aatgacggca ttgcctcttt
    15601 taatgatatc catcggtgcg gcacccaact tgtgttgcat aaccattcct tcattgatgc
    15661 tgcaaatcat ccctgccaag ttgtctaaaa cctcaactgc gccaatgtcc aagaggagtt
    15721 taagaatcct gccaaaaatg taatccccga tgagaatggt aaattgcagc gcctgatcat
    15781 agatttgtcc ttcttcttcg tccttgacct gatgatggat caggttggca aaatacaatg
    15841 ttttaaaaag gctggccatc tccatcgtca acttctcatc caaatcaagc tgtttgtatg
    15901 ccaataccac cacagccggt attaattgta aacttttatc atcctggagg cattcccgca
    15961 gggcagtcaa atcatcccac cagatctgca gtccttcctg atacaggacc tcaacttgct
    16021 gcatttctgc ggatagaagc cgggcaaaat gatcattcat ccatttgttt cctctcattg
    16081 gatatctgtt gatatctgtt atcattccca aaacctgctg tctatcattc tacaggatat
    16141 gcgaagttct gcctattaaa tttttcttcg taatgattat aatgatttcc ttcacaaaaa
    16201 aacaagacct cacttgttat cctcaagcaa ggtctttgtt agccgtgttt attagttttt
    16261 tccctgtatc ggtctactaa aatagtatcc ctcctaaatg tttaccacca agcgttcttc
    16321 ctgcacaggt acatatcccc ttttgatttc ggaagcctga acgtcccccg ttccgaattc
    16381 taccgccatg taatgacagg cgtcccaggg attaacctgt tctccgcaag tgaacacatc
    16441 aactgctgca taccccaact ccggccaggt atggattgcc agatgggatt ccgaaataac
    16501 aacaactccg cttacacctt gtggactgaa tttgtgaaaa accacttctc ttacttctgc
    16561 accagcttcc aaagccgcat tgatcatgat atcctggact ttattgatgt cattgagcat
    16621 ttcgaatcga cacccataaa attcagccag aacatgacgc cccaaagcct gcaataatgt
    16681 cgcctccctt tttagttatt cggcttgttc tcaaaccccc tttatttaaa cccgaacata
    16741 catgtagaat tttagcgata taaaagtgaa ttgtcaatat cgccggccat ccatgttccg
    16801 ggaaattctg tatgtattca tactatgccg aaacaacctg gcaggtgcag gttttgtaca
    16861 tgcaataaac agtatgctgt ttggctgccc ggcctagccg tccaagccgt ttatgatatt
    16921 tttaatcccg tgggtaaatt ccttttgatt gccttccccc agcagatcat acaagcgtga
    16981 attgaaagcg atcccaatct tgctcacttt ttccgcaata tcacggccct tgtcggtaat
    17041 aaacacccga attgcacgac ggtcctggga atcgagcttt ctgaccacca attcgtcttt
    17101 ttccagttta tcaacaagca cggtcattga agaactgtca attaaggttt tgttgccaat
    17161 ttctttcagg gtcgcaccat cttcctctaa aagactgtaa agaataaacg attgcgggat
    17221 acttactcca tatggatcca actgctgact caaagtacga tcaattttct tcatgaccac
    17281 ccgaaaacta aaccccaaat agctattcaa atccaacaat tttttggcct ccttttatta
    17341 cgttctgata attctgaatc tttaaccctt ttgcagaaca ccgtgaatac agtttcaacc
    17401 acggacatac tttattgcgg atatattatg gaaatgtatt ttaaatattt taaaaataca
    17461 tacattaatc atattacaga atattttgat ttgcaattca aaatcttctt aaatttatat
    17521 aaatgttaat tataaccaaa cagacgtact tgaaatctat tcgttttgat ggtattttat
    17581 atgtcaagtg caataaaaaa ttgccagcgg cgatgaaaga gacaagtaag cagtcccaga
    17641 gccaaagaga accggtggtg gtggaaatcc ggtgcttatg aactgctgaa tggactcggg
    17701 agtttcagtt ccatgtgtac tgacggtatt ccccgttatc ggatagggat ttattcctgc
    17761 caagtgggcc tttttgtcaa taagggtggt accgcggatt gtttccgtcc cttacgcgat
    17821 gaaaggtttt tatttttgta tacggagagg atgatacctg tgaaaaaaag aatcctgact
    17881 ggtgatcggc ctaccggcaa acttcacctc gggcattatg tgggcagtct tgccaatcgg
    17941 gtgaaactgc agaatgaata tgaaagtttt atcatcattg ctgacgttca ggcactgaca
    18001 accaatttcg aaaatcctgc caacctgagc cgggatacgt ttcaaatcgc cgaagactat
    18061 ctggctgtcg gtcttaaccc tgaaatatcg acaattttta tacaaagtat gattccggag
    18121 atccatgagc ttaccatgta ctattccatg tttacttcgg tcaatgtact gcggcacaat
    18181 cctacggtca agaccgaagc ccagcagcac ggctttagcg acatgactta tggttttctg
    18241 ggttatcccg tcagtcaggc ggctgacatc agtattttca aagccaacct ggtgccagtg
    18301 ggcgatgacc aaattcccca tattgaatta acccgcaaga ttgtgcgtcg ttttaatgat
    18361 ttatatcaac cggtgctggt ggaaccggaa gcgctgatcg gagaagtacc ccgcttgatg
    18421 gggctggacg gcaaggccaa aatgagcaag agtctcaaca atgcgatcta tttgagtgac
    18481 agtgctgctg aagtggaaag caaggtaaaa gacgcggtca ctgacccggc ccgaattcgc
    18541 aaaaccgatc ccggccatcc ggacatttgc atggtatttg cctatcacaa ggtatttaac
    18601 ggtgcagcaa cagaagatat tgaaaaagcc tgccggcagg gagaaatcgg ttgcgtgcaa
    18661 tgcaagaaaa agctcgcggg ttgtatcaac cagttgcttg aaccgatgcg ggaaagacgc
    18721 attcaatatg aaaacaatcc ggaaaaggtc agtgaaatat tgcaggaagg cacccgccgg
    18781 gcccgcctga cggctcacga aaccatgcgt gaggtgcgtg aagccatgca gattgattat
    18841 tttggcccgc gatgaataat tctgtctcgt atatctatta tcatggcaaa gcccgtcagc
    18901 ttaatcaaaa accggcttgt gaaaagccgg tttttttatc atctaatcaa ttttcggggg
    18961 tgattgcatg attctttgct ccagttcagc cagacggctg atctgtaaat acaagctgcg
    19021 aaactggtcg ggagtgagtg attgctgtcc atctgacagc gccgcttcgg gatgctggtg
    19081 cacctccacc aaaacgccgt ccgccccggc agccacggct gctttggcca ccgggcccac
    19141 catttccacc cgaccggtgg catggctcgg atccaccaaa atcggcaaat gggacagatg
    19201 cttgatcaga ggtactgcgc cgatgtcaac cgtgttgcgg gcatagggtt caaaggttct
    19261 gatcccacgt tcacagagaa ttatcctggt gttgccacgg tccagcatat actccgccgc
    19321 cagcagccat tcttcgatgg tggctgccag accacgcttt agcaacaccg gtttgtcagt
    19381 cctgcccaat tcctccagta aggaaaagtt ttgcatgttg cggctgccca cctgaaaaat
    19441 gtcaatacaa ggatacaaca cttcaatatc cctgacatcc ataacctccg tcaacacggc
    19501 cagtccggtt gcccggcggg cttcctcaac aattcttagt ccttccccgc ccaagccttt
    19561 aaatgaatag ggggacgttc tgggtttaaa caaaccagcc cgtaaaacat tcaccccgat
    19621 cgattgcaat atttgggcaa tctcaatata atccgactga ttctctactg cgcagggacc
    19681 ggctatgaca gtcatgtaac cggcaccgat gcagaccttc tgccgcggac cgtgtatttt
    19741 tactacggta tccggttggc tgtggttgcg ggcagccaag gtaaagctag gcattggttt
    19801 tcctccgtgt atgacgacaa aagcaattcc aatttcgcca ttgatttcat ttataataag
    19861 tatagcatga gccaaactgg aaattgctat gtaatgaagt ttttttatgg gaaaattgtt
    19921 tatcgggcag gccaatgcca gcccggaagg gagatgaaaa tggacaggat gattgatatc
    19981 gccatatgtc agatgcaaac cagccgggat aaagaagtca atctgcgcaa ggcggtccag
    20041 ctgatcaaaa ccgcagcccc caaagccagg ttgctggtat tgccggaaat cttcaatgct
    20101 ccatatcaga ctgacctgct ccccgcttat gccgaaccgg tacccggacc tactacaaaa
    20161 atgctttccg atctggcccg ggagcatcgg gttgtgttgg tgggcggttc cattccggaa
    20221 atcgcagcgg acggtaatat ctacaatacc agttgtgtct ttgcagcgga cgggagcatg
    20281 ctcggctgtc atcgcaaact tcatcttttt gacatccaca tccctgacca gattgttttc
    20341 agggaatcag atgtatttac cccgggggaa gacttgccga cctttatctg tgagggcatg
    20401 tcgtttacgg taatgatctg ctacgacata aggtttccag aactagcccg ccaggctgcc
    20461 ttgaacggaa gcagcctcat tatcgtgccg gccgccttta acctcaccac cggtcccgcc
    20521 cattgggaaa tcctgatgcg cacccgtgcc ttggacaacc aggtttttct ggccgccgcc
    20581 tccccggctc gaaacccggc agccagctat caagcctggg gtcattcctt gatcgttgat
    20641 ccgtggggaa ccatactggc cgaagcagga acgggcgagg aaattattta tgcccgaatt
    20701 gatccggcaa aactagcgaa agttcgagcc gaactgcctc tgttgcagca tcgtcggccg
    20761 gaattgtatc gaaatatcag ccgaaaggag tgttgaggat gaaatttcat accgaatacc
    20821 tatggtttaa tacggaaaaa cgccgccagt acatttgcat aacagaacgg gttagagagg
    20881 cctgtcaaaa aagcggcatt caggaaggca tgatcctcgt cagtgccatg catatcagcg
    20941 cgggggtgta tgtcaatgac cgggaagacg gaatcattca ggatctggat gaaatgctgg
    21001 agaacattgc cccgctgggc aaggattacc tgcaccatcg caccggtgaa gacaacgggg
    21061 atgcccacct caaaagcatc ctggttcatc accaaatcat cgtgccagtc accggcggcg
    21121 atctggacct ggggccctgg caggaaatct attacgctga atttgatggc cggcgacgca
    21181 agcgggtcat tatcaaagtc atgggagaat agtcaatcag actgcaaatc cggtcgtaaa
    21241 atttttgccc catgcaaata aacatgcctg acctgctcct ggctgtaatc cgtgttccac
    21301 aagatcagca ctcgtatgca tagcggcagc gccccttcga tctcgatttc cgggaaacac
    21361 atcagcggca cccggtccca accaagctgg cgcgctgccc gggcaggaaa aaccgaatga
    21421 atgtctgtgg ttacggtaaa caggactgca gccagatcct gcacggaaaa atcattggct
    21481 tgctgcattt gtgtcaacaa atcggtggtc gcagcaagca cttccgcttg ttgatctgaa
    21541 gcaatggtgg ttgcgcctcg tattcctctc actgtcataa aattcaacca tccttcgcga
    21601 gtcatcaagc tactgcccgg tgccccaatc caatcgcgac ttcatccaga tgcaacagac
    21661 caataccgaa aaatatggct acgctgatgt ggtcaccatg acgggcgatc cctatttttc
    21721 cacccacatt caatcccagc gccttgggca ttatctgtgc cagcgcttcc cgggtcgccc
    21781 cggctaccgc cccttcgtct gcatgaatgt ccttaataac cccttcacgc ttggcagcaa
    21841 ctactgccct ttccacaatt ttggcggctg atgtgataaa ttctccacca tagtccaccg
    21901 ccactgcccg gatcgcaaac tcctccagca acgacttttt aagtattcct tcctcttgtc
    21961 tggacaagct caacgacatt ttgatcgctg cggttgcgac catcttactg ccaacttcca
    22021 tcctgcctac caccctgctt ttttttctat tttactataa ttttaccgat catgactata
    22081 ctctggttgc cgctgaatac ctgccctggt tttggataag ccagattccc ggcggcagta
    22141 tcaattgcaa aggtcagtgg ctgagaacaa ttgcgggtat caatttccac cgtatcgccg
    22201 ccattgaccc gcagggtcac ctgggaccgg gaaaaatgat aaaggctttt tccgttgatc
    22261 aaaactttcg ccgccggagc agactgtgcc tgaaccagct tgatggtcag cagcgtctgg
    22321 gctgcggaaa gatcatcctc aatggtcgtc aaatacaaat cttgattagc aaccaccggc
    22381 gctggcagtt cctgtccttc catcctctcg ctccagctca tataaaaacg tgtggcatca
    22441 tcggtcatca atccctgagc taccaccacc gccaccaaac acaagaccac cagacgcatc
    22501 agcaaggtct ccgccttatg cagcatgttg accatctcct caagcatttt ttctatcata
    22561 tgcctgccga tggaccttta tccatgcatc acgaaattct attttccgat tttccgtttt
    22621 ctgctcaatc tctcgatttg agaatagcca acgccatggc tgccgctcca aaaccgttgt
    22681 cgatgttgac caccgcaacc ccgctggcac agctgtttaa catggccagt aagggagcca
    22741 acccttgaaa gctggcccca taaccgatac tggtaggtac tgcaataacc ggacgggaag
    22801 tcagtccgcc caccacgctg gctagggcac cttccatacc agccaccaca atgactgctt
    22861 gactggcgtt gatcattggc attttgtcaa agagccgatg aatccctgca actccggcat
    22921 catagatacg ttcaactttt acccccatga tctccagcgt caaggcagcc tcttctgcca
    22981 ccggcagatc tgcggttccc gcgctaatga ccgccacctg cccgggacag ggcaaatccg
    23041 atctgcacag ataaagcaag cgggcaacgt catcatactt cacctggggg atgctttctc
    23101 ttattcgatg atatacggct tcctctgccc gggtcgccag cacatgatca gattgttcgg
    23161 ccaaccggcc aaagatttgt tcaatctggg ccaggctctt gccgggacag taaatcactt
    23221 cgggaaaatt gtcccgcaag ccacgatgat gatccagttt ggcaaagccc aactcttcat
    23281 acggcaggtt tttaagcttg tcatatacgt cttccagggc cagttccccc tgctgaagcc
    23341 tggtcaacaa acgatacaat tctttttcgt tcatattttt ctctttccgt ttttcaactt
    23401 gtgggacttg tctggatgca aagcaaaaac cttttacgct atcctaatta aagcgcaaaa
    23461 ggtcgcttga ttcaatctct tccttgtggt ttgcatcgaa tatgaattac caatgttgcc
    23521 gggcatagct caccccagtc agaaaagctt ctttgttgct ctcaatcacc gaaggctttt
    23581 tgcctttata aatccgctca aaatattcca gtactttttc agtttccacc acttctgtct
    23641 tctgtaagta agcccccagc aagatcatgt tggctcctcg ggggttggca atatcttctg
    23701 caatctggtt gacgggaatg gataaaacct ccacatcctg ccgctgcggt tggcgatcca
    23761 ccagggatga attcaatacc agcgtaccgc cgggctgaat tttttgttca aacttctcca
    23821 gcgaaggtcg gttcataata accgccatca aagggttttc ggtaatcggc gagctaattt
    23881 cctcgtcggc gatggtcacc agacagttgg ctgtaccgcc gcgcatttct gcaccatagg
    23941 agggcaccca ggcaacaaac ttgcctgctt ccatggcagc ataggccagg aattgcgcca
    24001 tggacaggac gccctgtcct ccgaatccgg caaacaaaat ttgtttttcc acttattcct
    24061 cctccttgac cttgaattcc cccaggggat agtaaggaat catattgttt tccagccagg
    24121 ccagcgcatc cagaggaccc agtccccagt tggtgggaca agtcgacagg atttccacca
    24181 tcgtaaaccc tagcccggcc tgttgtatct gcagggcttt tttgattgct tttttggtgc
    24241 gcatgacatt ggccggattg tggaccgagg ttcggcatat atacgccgaa ccttcgcttt
    24301 gtgccagcag ttcggccatt ttagccgggt agccttccca ttctttttta cgcccataag
    24361 gagaagtggt tgtcacctgt cccaacagag tggtaggtgc catttgcccg ccggtcatgc
    24421 cgtaaatggt attgttgaca aagataacgg tgattttctc tgcgcgcagc gaggcgtgaa
    24481 tgatctcgcc catgccgatg gaggccagat caccgtcacc ctgataggta aaaaccgttt
    24541 tatccggcag cagccgttta attgcggtgg caatggccgg ggctctgcca tgggcagctt
    24601 ccagcatgtc acagttgaaa taatcataag ccagcacgga acaaccgacc ggacatattc
    24661 cgatggtttc ctcccgcagc tcaagttcat ccagacattc agccaccagt cgatgaataa
    24721 tgccgtgtgt gcagcccggg caataatgaa atttattttc tttcaaactg gcggggcgtt
    24781 tggctactac cttcatcaga acccctccct ttctgcaatt ctctcgattt cccgcaggat
    24841 ttcttcggga ttgggagtga tgccgatgcg tccgtagaaa tcaaccggag cctttccggc
    24901 cacagccagg cggatgtctt ccaccatttg ccccatgctg atctccactg acagaaatct
    24961 ccgggcggtt tgggctgctt taataattgg ctcatccggg aacggccaga gggtaaccgg
    25021 acgaatcatg ccaactttca agcccttttt gcgggcatcc ataaccgctc ttctaacaac
    25081 tctggccatc agtccaaaag ccacgaccac caagtcggca ttttccgtca gataggtttc
    25141 tgctcttttt tcccgacgca ttatttcatc gtatttgctt tgcagatgat ggttgcgttc
    25201 ttccagaaat tccggcgaga gatgcagggt attgatgaca ttgggcgctc gtccctgaca
    25261 gccggtggta gcccatggtt tttcataaaa ttgttgcacc ggttctcgaa tcagaattgg
    25321 ctccatcatc tgccccagcg ttccctccgc catgatcatg actggtgtgc ggtagcggtc
    25381 ggccagatcg aaagcctcaa tggcaaagtc catcatttcc tgtaccccgg aaggtgccag
    25441 aacaatgacg cgatagtcgc cgtgtccccc tccccgggtt gcctgaaaat aatccgactg
    25501 ggccggttgg atgccgccca gaccaggtcc gccgcgggaa atgttgacaa tcaagcaggg
    25561 cagttcagct tccgcaataa aggatatccc ttcctgtttc aaactgatac ccggaccgga
    25621 ggaagcagtc atcacccgat gaccggtagc ggctccgcca tagaccatat taatcgcagc
    25681 cagttcactc tcagcctgta caaaaacgcc gtccagcttg ggaaatttac gagccatata
    25741 ctcagcgatt tcgctggaag gggtgatggg atagccaaaa aaggctttgc agcctgcaat
    25801 caacgcacct tcagccaggg cttcattgcc tttcattaaa accttttgca tgctacttct
    25861 ccttttccac cgcaataatc aaatctggac acatcaaggc acatatgccg caaccggtgc
    25921 attcttccgc tcgcaggata ctgacagggt ggtaacccag aatattgatg accttttcat
    25981 ccagtcccag aataccttgg gggcaaaagg caacacacaa ctcgcatgcc ttgcattgat
    26041 caatatgaaa ataaacccgg cccttggcca tccttctcat cccctttatc tacacttatt
    26101 gcagccaggc aggtctcaaa taacgtgtga tgggctgtaa atctttgccg aaatcatgaa
    26161 ccagatcatc ataaaattga tcttcggcag ccagccagct gaccggcaat tgcatggcct
    26221 gggcataggc cgttacaatg tggtgcccgt tcctgatcaa gtccggattg gtttcagcca
    26281 ttaagttggg gttgctgatc aagccggtaa aagttaaatt ggccgcgttt tccagcaata
    26341 ctcgcaattc catgaccaaa tccagattcc cggcaaaggg ccggtacgga ttcagtacca
    26401 gataaaaccg ataatcgctg tccttaatat agcggctcaa gtaacccaaa accatggcgc
    26461 ccacttcatc gccggccaca tcaataacaa tctcaacatc ttcccgcaaa aagccaataa
    26521 tctccggagg aacactgggc acatctccca aagacagttc tccggcagga gccagcagct
    26581 tgattccccg ggcttccagc atatctttga cgtttcggga agcaaaatag ggattgacca
    26641 aatccagatc ggccagaata acgttatccg cgaaatggct tatatccaag gcataattaa
    26701 tggccagttc ggacttgccg ctgccgaacc cgcccgcaaa tattgttaca gttgccaaac
    26761 ttatcctctt cctgcataaa attcttattg agtaatacta cctgaggatt tttcgaaaag
    26821 caataaaaaa tatacccgtt taatgaaacc ggctgcttca taatcatcca gttcgtcact
    26881 tctccgttat gccaaaccaa aatccctccc ccgcggagga cggcaatcag ttgagattgt
    26941 ttatcctttt cagtaaatat tccttgtaat cctgcagttc ctcattggct ttgtataaga
    27001 tgcgctctac ctcggctttg gaaagggtca catcttcggg ctcgagaacc gtaaccgtac
    27061 agaatccttc cttcatagaa aacttgaggg cgtcctccag gtggtcggca gaaatggtga
    27121 tgaacactgg tgcataagca acttccttgc ggttaacctc gtcaaagacc gtatagactt
    27181 cactgctcat atcgatgtct tctatccgga tcccctgact gggcacattt ctcaacaagg
    27241 caactttctg ttgtctggtc tcctccgcga tttgttcaat gctttttttg ccgaacacct
    27301 tgccggattt ccccctgccc gggtagtcaa acctcagttt gatcttgatg gccatcagtt
    27361 tcatctcctt ctctctcgta cccgtctgtt cccctggcac aatctttaag cgctacgact
    27421 tcctgatcag tcagcagccg gtaatgtcct tcctccactc catgcaggga gaggaaagcc
    27481 agaccggttc gcttcaaact gatgacaggg tgaccgatgg cggcacacat tctttttatc
    27541 tgccgtttgc gaccttcatg gatcgaaatc tccagcaagc agtcgccccc ttcatacagc
    27601 aacttaacct gggctggtgc cgtcaggccg tcttccagca gcaaaccctg gcgcattttc
    27661 tgcagtttca gtgcactcac ctgccccttg accagggctt cgtaggtttt gatcatttta
    27721 tagcggggat gcatgatcaa gttggcaaat tccccgtcat tgcttaaaag cagcaagccg
    27781 gaggtatcat aatccagtct gcctacggga taaatgcgcg cttccatatc tcccagcaaa
    27841 ttaagcaccg tcgggcgtcc ttgggggtct tcaacactgc agataaatcc cgccggcttg
    27901 ttcaacagca cataaaccaa gcggggtaag cagttgttta ccatctgccc gcggtactcg
    27961 acccgatccc ggccaggttc cacttggatg cccatggcgg tgacctgttg tccgttcacc
    28021 ttaatctcgc cgtttttgat catctgctcg gcatgacgtc gggaggcgat tccagcctgg
    28081 gccagatatt tggctaatct caatgcacgt tcccttcctt aaaaagaaaa aagtccgttt
    28141 cgctcaacac gatggtatat tttttcattt tagcattctt ttcaacagct gttaatctct
    28201 ttatggtcta acgttccagg cgcaaccatc tcggcgttgt ttcaatcaac gaggttcggt
    28261 aatctcattg taaagcaaga cttgcccgac cgatatctgt tatgataaac aaggaaacga
    28321 aaaatatgcc tggaggaaag ccaatggaca aaacctttac cgtcgacaaa gaacgcattg
    28381 aaaaagcaat caaagaggtc ctgctggcca tcggggaaaa cccggaaaga gaaggggtta
    28441 aaaacacacc tgcccgggta gcccggatgt atgaagaact cttttggggc atgaacaccg
    28501 acccgcgtga ctttctggac gtttctttta cggaatacca cgatgagctg gtattggtca
    28561 aggacatccc catctattcc atgtgtgagc atcatttttt gcctttttat ggcgtcgccc
    28621 atatcgccta cattccaaag ggcggcaagg tagtgggaat cagcaaactg gcccgggtgg
    28681 ctgaagccta tgcccatcgc ccgcagttgc aggaaagact aacctcccag atcgctgatt
    28741 gtattttcga aacactgaat cccatgggcg tcggggtggt aatccaggcc gagcatatgt
    28801 gcatgaccat gcggggcgta aaaaaaccag ggtctctcac cgtcacctca gccgtgcgcg
    28861 gtattttcga aacccggtca caaacccggg cagaattgtt ctcgctgatc ttaaaataag
    28921 attgtgagga gttgtcatga ttaagggcgt tttttgatgc cctttcggaa caagatgcac
    28981 ctcttgaata aatcaagacc gatggtgata ttttcatccg ccatcggtct tcgtttgctc
    29041 ttctatgatt ttaccgttgg gcgatcagcc cttggttgcg aaaagttatg ccaacaccag
    29101 ccgggttgcg gaaaagttta aaacaatcca acgatctccc cgctgggcag gatgtcgatt
    29161 ttttcagccg ccggcgtctt gcccagaccc ggcatggtca tgatggctcc cgccagggca
    29221 acgatgaagc ctgctccggc agctagtcgg acttcccgga cggtgactgt aaatccggtg
    29281 ggtcggccca gcgcattggc atcatcacta agtgagtatt gggtcttggc catgcatata
    29341 ggaagatttc cgtagccaag ttcttcaaaa cggcgaatca tattgtctgc ttccttgttg
    29401 tactgaactc cgtcggcgcc gtaaatctgt ttgcaaatgg tttcgatctt ggctttgacc
    29461 ggcatttcca gctcatacag gaatctaaat gtgctggggg tttccgccgc tgtaatgact
    29521 ttacgggcca attcctcacc acccgcgcca ccttcggccc aaacccggga tactgccatt
    29581 tcagcaccca gcgcccggca tttctctctg acaaattcga tttcttccgg cgtatcgccc
    29641 gggaattcat tgagggccac taccactgga ggaccgtatt ggttaaggtt ctcgagttgt
    29701 ttttccagat tgctgacacc tttggccagg gcttccatat tcacttcggc taacttgtcc
    29761 ttctttacgc cgccgtggtt tttaagtgct ctgacggtgg ctaccagcac cagggcatct
    29821 ggtttcaaac cggcaaaacg acacttgagg tcaaggaatt tttccgctcc cagatcggct
    29881 ccaaagccgg cctcggtaat tagataatcc cccagtttca gtcccatttg cgtagccatg
    29941 acactgttgc agccatgagc aatattggca aacggtccgc catgtacgaa tgccggagta
    30001 ttttccaccg tctggaccaa attgggtttg atggcatcct tcatgagcag cgccatggag
    30061 cctgctgcat gaagatcatt ggcggtaacc ggctggccgt cataggtgta ggcaaccacc
    30121 atgcgtttaa accgatcttt cagatccatt aaatccgaag caagacatag ttcggccata
    30181 acttctgagg ccacactaat atcaaaccct gactcccttg gaattccgtc ggcttttccg
    30241 cccagtccca aaacaatatt gcgcagtgcc cgctcattca gatccatggc ccgacgcaat
    30301 acgatctgac ggggatctat atttaggcgg ttgccctgat ggatatggtt gtccagcaat
    30361 gctgccagca gattgtgcgc tgtggtaacg gcatgaatat ctccggtaaa atgcagatta
    30421 atgtcttcca tgggcagtac ctgtgcataa ccgcctccag ccgcaccgcc tttgacacca
    30481 aagcttggcc ccagggacgg ttcccgcaac gcaatgatgg ccttttgtcc gaggcggttg
    30541 agcgccatcc ccaaacccac cgtagtggtg gttttccctt ccccggccgg ggttggattg
    30601 atggcggtta ccagcaccag tttgccgttg ggacggtcct tgattttttc ccatgtttcc
    30661 agggtaacct tggccttgta cttcccgtat aattctatat catcatcgtt cagcccaata
    30721 tagcgtgcaa tttcagtaat tggctgcatg atcgcctgtt gggcaatttc aatatcgctt
    30781 ttcaatctgc tacccctctc ataaatgttt ttacggtaat tgttttttat cttgccaatc
    30841 ttcacaattc tgcattatta taccctgacc aatcaggcaa gtccagccat gctatgaaaa
    30901 taaatgatgg catatataaa tggaagcaac caagccggtt atatctgcac tgagtcccgt
    30961 gagcaaggca taacggtagc gttttatccc caccgaaccg aagtacaccg tcaaaacaaa
    31021 aaaggtcgta tcggttgtgc cctgcatcac cgaggccatt ctcccgatca gagaatccgg
    31081 tccgtattgc cgaatcaact cggttgccag gcccagcgcg ccgctgccgg aaagcggacg
    31141 cattacggcc agcggcatta cttcggccgg ggctcctatt ttttgcatca acggcacgat
    31201 ggcagagatg acataatcca tcgctccgga agcacgaaag acagcgatcg ccaccagcat
    31261 tcccaccaga aaaggaatga ttttgacggc tgtataaaag ccttcttccg ccccttcaac
    31321 aaacgtttca tagaccggca aacgttttaa aaaaccataa atcggaatgg tcagcaggag
    31381 aaaaggaatc gcccagcggg aaacagtgtc caggatggcc agcatacttc ctacctccct
    31441 gaatggctca gatttctgaa cagccaatcc agggtcacgg caacaacact ggcgcagcag
    31501 gtggcaaaga tacaggcacc tacgatttct gtggggttgg ccgacccgta tgacactcga
    31561 atgccaataa tggttgccgg aatcagcgtg atacaggaag tattcagggc cagaaaggta
    31621 cacatggcgg gagaggcttg atcacttccg ccatttaact gctgcatttc ctgcatggct
    31681 ttcatgccaa aaggggtggc agcgttgccc agccccagca tgttggcgct gagatttaag
    31741 acaatggccc ccatggccgg atgcccggcc gggatggaag gaaaaagcca tttgagcaag
    31801 ggattcaaca gataggccag gattcggatc aaaccacctt tttcagcaat cttcatgatc
    31861 cccagccaca gcgccataat accgatcaaa ttaaagcaca cctcaacggc atcgcgcgcc
    31921 gcacccagcg ccgcctggct gaccacttca atattaccgt tgcaggcagc ggcgatgatg
    31981 cctgaagcca gcagcaaaag ccaaatcaca tttaacaaat ccatcccccc tgcttgcaca
    32041 aatcctgttt aagcaagtta tgaaaggggg ccggaaaata gaacaacccg cttttatgcg
    32101 ggctgtcatc caggcaggca aaggcctggc attattcgct gccgtgccct gctttaatgg
    32161 gctgcagttc cctctgcttc atggcggttg cgtcgttcca tcaagccttc caacttgtcg
    32221 atgagctgcg gggccaggtc gaccactctt tcggccaggg tattgccatt gacggaaagc
    32281 aatcgcacat cgttcatcct gactaccaaa aagcccactg gtttaacgga tacgcctgct
    32341 ccgctgcctc ctccgaaagg tatctcctgg ctcttattgc tgttgtcaaa ctcatactcc
    32401 ccgccgccgg ctgcaaatcc gcaagctacc tgggaaacag gaatgatcac cgtaccatca
    32461 ttggtttcta cggcatcgcc taccacggta ttgacatcca ccatttcctt aatgctttcc
    32521 atggcggttt tcatcaaccc ttcaatagga cggctgttgc cgaagttttg ctgcgattcc
    32581 atccatccac cacctgactt tccatactgt taaatagatt gttatggtga taatatgtac
    32641 gattcgtatt tttaatatac aatcaatttg actggcaaaa tcagtgctca aataatccgg
    32701 ttccaccacc agctgcacct tcttaaggtg actgtggcgg gtcagccagt tcatccccca
    32761 tcctttgatc atccacagca gcccggtata aatagctgcc cgcatggcat cctttccgcc
    32821 cacccggctt tcccagctaa atttttccat cacggtcaag gtcagaattt ttttcaatac
    32881 gacccccatt tgttgttccc agacggacaa caaatgcaaa cgtcgcggcc gcagcagctt
    32941 cttcaggaat tgcttccagc tgcttgtttg ttgggtttta aaactttttt gccagcgatc
    33001 catcagtaaa gattccagga aatgtaaacg cggatcggta tagtcataga tttgctcaaa
    33061 gcgataaaaa ggcaggccga aacgaatttg tatcccctga aaccgctcat tgccggtcac
    33121 catgatttct atacgaaagg taatgtacag cgataatacc agagccacag ccagaaaatt
    33181 aaaaacgata tggaatttca tcggcattct cccgtttttc catatcgttc cccgttattg
    33241 agcggctatg caacagatta tggttttttg gctttcgcaa tggtggtttc gatctctctt
    33301 acgatctgtt ccatctcccg ggcaatgatc agattgctgc gaccttgccg aaaggtggga
    33361 caatgcaaat tgtagtcaag gatcttcttg tcgattttct ccagatcggc ctttctttcc
    33421 atgaaaaaca attccttttt atattcaaac cgctgctgcg cccgctcgtc atgttgatcc
    33481 gtccagaaca cctggcagta acgtttgaaa taatcgattt cacggcacag tttgtcctgg
    33541 gctgcatcaa tctccttgcc caattcaatc caatacgggg caaaatcgtt gtctttcagg
    33601 attttattcg ccatgcgcat ttctgccggt tcaaatgggt tttcttccaa attcaacggc
    33661 ttgccggctc cggccagatt gtcaaagtct ccacgttgcc gggctttgat gatttgttct
    33721 tccaccaggt cgcgggtact gctcatatag cgagccagtt ttcgattttg ctgcgctttt
    33781 tgcatgattt cttcttccag gtctttgttt cccatggctg gctcttcctt tcctcatcat
    33841 gtcaatcgca accgcacttg cctggtcagt cgtttaaggt aattcgttct ccgggttagg
    33901 aagttcctcc aaactggaca ggccaaatat tttcatgaat tcgtaactgg tccggtagag
    33961 aatcggcttg cctgccgcct ctttgtgccc gtcttccctg ataatgcctt tttccaacag
    34021 gttgctgagc acccgttcga ctttgacgcc ccgaatttgc tcgatctcag cccgggtcac
    34081 cggctgtcgg taggctatga ccgccagtgt ttccaaggct gccgccgaca accgcaggcg
    34141 ggccggcttg accagcttgc tgagtatccc ggcaaactct ggtttggtgg ccataatata
    34201 gcctccctcc agggagatga tctgcaatcc ccgggaaggt gactggtgat attccagtat
    34261 caattcctgc ataatggagc gcagatccgc ttcctctatt tgcagcattt ccagcagttc
    34321 agctgtctcc aaccgttctc cgctgacaaa cagcaaagct tcaattgcgg cttttatgtc
    34381 ttcccgcatc agcatggtgc gctctcctgt agacgtaata ggatatcccc gaatcggctc
    34441 tcctgaaccg cttcgatccg attcaggcga atcaactcca aaattgccag gaacccggcc
    34501 agaatctccc gccgctgttg ataaccaaca aacatattat gaaaactggt ctgtttcacc
    34561 ttctgcagac actgcatgat ttcctccatt ttcgcgtcaa cattgatatc tccctgggga
    34621 acacaatagc ccgctgtctc cagctcgtct tcctggagcc gcaaatatac ccgcagcagc
    34681 gtttgcacag aggctacaat ttctttggcc ggttcgatca tgtccagttg tccatggcga
    34741 aagaaaggcc gcggataatt ctcatctaat cgtaatgcca accattccgc cgccttttta
    34801 tagcgtttgt aggcgatgag ccggctgacc agttcttcgc ggggatcggc ttcctcgtcc
    34861 tctgttgttt ctggtttgcc cggcagcagc atccgggctt tgaggctgag cagataggag
    34921 gccatcatta aaaattcacc aatctgatcg agttccatat gtccgctgcc ggtaacaaaa
    34981 tgcatgtatt gatcggtgat gatggcgatg ggaatatcgt aaatattcat ttcattcttc
    35041 tctaccagat aaagcagcaa gtcgaggggg ccgtaaaagt gttccaggtt gactacataa
    35101 tccacaggca ttgccctccg ctaaaaaact gtcccgtaaa taatagtgta aatcatgttg
    35161 tacagaggcc agataatttt tccggctaaa tcggtaaaaa tgatcagtaa cagcagcaac
    35221 gggccaaatt gctccagaga ataaataaaa ttggccccct tgtccggcag gatccctgcc
    35281 aggattttgg aaccgtccag cgggggcacc ggcagcaggt taaacaccgc cagtataatg
    35341 ttgatctgaa tcagcggaat gagcatctgg atgactgctc ccagagcgcc gccgtagaga
    35401 ccggtttgcg gatcaaaacc gatcaacctc aataggatca taccggcgaa ggccaacagc
    35461 agattcatgg ccggaccggc aaaagagacc agcatcatgc cggttttgat actgacgttt
    35521 ttaaagttat aggggttgac ctgcaccggt ttggcccagc caaaatgcag caacagcaat
    35581 gcaaaaaagc cgatccagtc gatatggggc aacggattga tggtcagccg cccctggtaa
    35641 tagggagtgt cgtctcccag ccagtcagcc acccgtccat gagcatattc atgaaaagtc
    35701 agcgcaatca aaattgccgg caaggtaatc agcatgttat atatgaattg cattgggtat
    35761 gctcccttct tcgagccatt gtttgaccat ctctatttct tcctggcggc tcatcagctt
    35821 tgcatccagc ttgtattcat acagcctttc caaaatcagt ttaaaagccg gtcccggctt
    35881 cagccccaag tcccgcaaat cctgaccatt gatggcaatg gtcacgcgtt ccttctgatc
    35941 caggtattgg ttgatccggt cccaggcccc ttcatccttg atgcacaaca acaaatacag
    36001 cttgttttca ttgtccagcc cggagagcat gcggtcaaaa tcactgggca ccagcaaccg
    36061 gttttcactg atccgcgccg ccagctgagg aatggcggcc gcatcaatga tcgctttttt
    36121 ggcggtacgg tcaatgggat aattctgcag gatctcttcg cggctttccg catttacctg
    36181 ggcaaagaga atcagcaaat agatcagcca ggttctgaat ctccatccgt agtatctgtc
    36241 caaccaccag ccggtgacca gcggtactct tttaaattta ctccaactga ctttttcaaa
    36301 attgatttcc ggcagaacat atttccatac cccgatttcc tgcatccgcc gcaatgctgg
    36361 caaagggtcc ttttcgttca gaatgagaat caactcggaa aggatccgtt tatgggaaag
    36421 tttgcccaac aagcggcgtt caatggcatc ccgggcaaat ctcattgtat ctgtttcgat
    36481 cttgaattga tagcgctgtt caaaacgaat cgcccgaatg attctggtgg gatcctccac
    36541 aaagctgaaa ttgtaaagga tgcgaatcaa cccctggcgc aagtcgttgc tgccgccgaa
    36601 gtaatcaatc aattctccaa aatgtccggg attgagacag atagccaagg tattgatggt
    36661 aaaatcccgc cggtacatat cctccttgag cgaggacttc tccacctgag gcagggctgc
    36721 cggaaactca taatactcag tacgggcggt ggctacatct attttgctgc catccggcag
    36781 aatcaccgcg gcggtgccaa acctttcgtg ggtacgcagt ttggctttaa aagtgtccgc
    36841 cagcgcggct gccagtctca atccatcgcc ttccaccacc agatcaacgt caaagttgct
    36901 cacctgcaac agcagatccc ggacaaaacc acccacacaa tacacggtgt aatcaagccc
    36961 ggcagcaatc tctcccgccg tgcgcaagaa gttcagcaat tgctgcggca gttgtcggtg
    37021 catttgttcg ctgcagtttt cccaggaacc ctcggaataa gaatacagca attcatggtc
    37081 ttctggataa tcgtccccgt gcagcgtacg taaaatatca gtccgggaaa cgataccaag
    37141 caacctgtct tcctcaatca ccggcaaccg gccaatgtca tgctccacca tcagcttttg
    37201 aatatcactg accggcgtac tggctgtaac ggagagtacg tcagccgtca taaagccctt
    37261 gaccggggcg tggcccaaga gatgaatcct ggctttgtct acatcccggc gggagatcat
    37321 gccgatcatt tttccatttt cgaccaccgg catgccggta tgtccgtagc gaagcatcaa
    37381 tctcccggct tcctccatac taatggtgga acgcagggtt ttaaccgggc tggacatgat
    37441 atcggcagcc gttatggaag gatgaatcag taatttcagg ttgtcaagaa tgatcgcttc
    37501 cactgccgcg cattcctggg agcgcaccag agctgacgca gcttggtcat gtccccttcc
    37561 tcccagtggt ttgaggacat cattgacttt cacctgactg ctgcgactcc tgccaatcac
    37621 atgcaccttg ccttccattt tggcaatcac aaaagccgca tcacagtttt ccatctcaaa
    37681 caaacgatac gtcaccagat ccagacctgg aacaaactcg gttcccgaga aaattgccgt
    37741 aacgatatcc caatccttga tcttgtaatg ggtgccggct tcaatcagct tgtgaagcgt
    37801 ctggcgctgt tcttcggaaa atggctgttc cataaaacta ctcactacag ccagattggc
    37861 cccgcaactc aacaaatagg cgaccgccct ggcatcgcgg gaggtggtgg aaggaaacag
    37921 caaactgccg gtatcatcat aaattcccaa ggaaaaaatc gtcgcatcga aaggactgat
    37981 ggaaatattg cgctccataa tcttttccac caaaatggtg gtagctgccc cggtagggtg
    38041 aatctcgacg atactgccgc gcatatcatc ttcgctttgc ggatgatggt catagatgtg
    38101 aatgtccagc cccgggttat tcaatacccc ggacagttgg gccagccgat ggggactggc
    38161 agtatccacc agcaccagtc tttgcacggc tttaacatca atttctttcg cggtcttgat
    38221 ggccagcaaa tctttataca gcgacataaa ccttttcacg cttttattga cggtaccgga
    38281 aaagacccgg accgctcccg gatataactt ggcggccgct gccatacagg ccaggccgtc
    38341 aaaatcaagc gcattatgcg aagtaataat ttccaaagtc attctccaat actcaaacta
    38401 actgcattat agcataccgc taccctgctt gacacttgtc gccttaatac ccccaccctg
    38461 cagcaataaa aaatccgctc aagcaacgtc tcccgacata tcttgcgcgg atggcagcaa
    38521 atgatttgtg actgatcgtt tttttaaggg atgtttggct tgtttcccgc aggtgcatca
    38581 gtcattttgg ctctcaaagc aataatttcc ttttccagtt ttttattgta atgagtcaac
    38641 tcgtttatct ttcgtcctgt tttaaaagcc ctgaagctgt caattaaaaa agtaatgatc
    38701 gcgcccccca aagcagcaat tagagcaacc aacgccagtg aaacttctgc cgtacgccag
    38761 ctgataaatt gaatcgtcac cggcgtattg ttctgtaaca caaaaacaac aatacaaatc
    38821 ataaatacca aagctccaac caaatatgcc ggcataaatc cgttccccct aacatattta
    38881 tatcaaaagc attatatact gattattgta aggattttgg cttgcaaagg gaagcaaaat
    38941 tattgtaaat tgctatatat ggcacccggc catgctataa cacttgcaaa taaaaccaac
    39001 ttgagataaa ataattaaag ctatatatat tcaagtcagg ttaatttaac caggctcgca
    39061 atgggcagcg gaataccgcg ggacttccaa aaacagtgga gtcccgctat ttttataaaa
    39121 gggagttaga ccttgggcgc aataaaaaca gtccgtacgc tgtgggcgga tgaaaaacaa
    39181 agagtcgctt tcatttcgat cctttcgaat actaccttgg tgttgttaaa actgatggcc
    39241 ggaattctga ccagctcggt cagcatcatc tccgaggcca tccattccgg gctggatctg
    39301 ctggcggcca tcattgcttt tattgctgta cgtatttcca gtacaccgcc cgacgacaaa
    39361 catcattacg ggcatggcaa atttgaaaat atgtcgggaa cgattgaagc gctgctgatt
    39421 tttgtcgccg cgatctggat tattgtggag gcctacgaaa aaatcatcca cggcgcgcaa
    39481 gtggtaacga ttggaatcgg catcgcggtg atggcttttg cctcgctggt caactggctg
    39541 gtatccggat tattgatgag aacagcacgc aaaaccgact cgattgcctt ggaagcagat
    39601 gctctgcatc tgcgcaccga tgtttatact tcgttgggcg tcatgttagg tttgctggtc
    39661 attgcaatca ccgacatcca gattctcgat ccgatcattg ccattgccgt tgccctgctg
    39721 atttgcaaag ccgcctacga tctgacggtc aaggctttga tccccttgtt ggatgtcgct
    39781 ctcccgcctg aagaagaggc aatcatcaaa gaagcgattg attcggtaag tctggacagc
    39841 ctggtcaatt ttcatcagct gcggacccgc aaggcaggcg cggaaagata tgtcgacatg
    39901 catttggtct tcccccgcaa tttatccttt gctgcagtcc acgacatgtg tgaccgtatt
    39961 gaaaatgaaa ttgaactgcg gctcaaccac gttcacgttt tggttcatgc cgaaccttgt
    40021 gaagacgccg actgtgaact gtgtcgtaaa aaagaatgtg atatcaaagc ggacggtgat
    40081 taaccaatcg gctggcaaag gggcatagga ttaagagcga caaagcaaaa aggcaaacga
    40141 aaagcccgtc cgaccagacg ggctttctta tgctatacag tcatggcttg tgccctccgc
    40201 cgctgactag ctggcggagc gttgcttgat atcatagaat aacgggcagg caacgcatat
    40261 ggaagaatac ataccgtaga taaaaccgac cagcagggcg atgacgaaca ccttgatggt
    40321 agaaccgcca aacagcagca aggtcaccaa cggcattaaa actgtcagta ccgtattgac
    40381 ggaacgattc atcgtctgca tgatgctgcg gttgagcaag gtatggagct cttctttctt
    40441 gcgggtgcgc aggttttccc tgacccggtc aaagataacg atggtatcgt tgatggaata
    40501 accgatgatg gtcaggatgg cagccacaaa gtagctgctg acttcccatt ggaatataga
    40561 gaaaagtccc acgaccacca aaacaacctg ggccagcgaa agaactgcgg ccagaccaaa
    40621 ggcaaattca aagcggaacg taatatagag cagcatgaga actaacgcaa taccgatggc
    40681 tatcagcgca ttacgggcta tttcgctgcc aatggtagca ccgacgcttt ccgcactttc
    40741 cagggtaaca ttggtgaatt tctccttcag cccggcagtc attttagcgg tctgctcctg
    40801 atccagttca ttggtacgaa gatagaattc gtctccgctc ttttgcacgc tgacctcctt
    40861 gaccaggttg aactcgttga cggcattcct gacctgatcg gcagagatgg agctgtccat
    40921 tttatagcgc agcaaagatc ctccggtaaa gtcaatgctc caattgagtc cctgcatgaa
    40981 caaggaaaca atcccggcaa caatcagaat tgaggagaat atataaaacc attttcttct
    41041 ttcaataaat tgcattttct accctcctta tacgccgtat aatttattgt tcttggtgat
    41101 atcggcaaac cagaccagca tgtaacgggt aaacgtcaaa gccaccagca tgctggccac
    41161 aataccaatg gagagggtaa ctgcaaatcc tttgattgaa ccggttccaa accaaatcaa
    41221 caccatcgcc gcaattagtg tggtcaggtt ggcatcgaag atcgtccaga acgcccggac
    41281 gaaacctgat tcgatggccg ccttcaaggt tttgcccaac cgcagctctt ctttgatatg
    41341 ttcgtaaata atgatattgg catctaccgc cataccgatt gataacacaa aagcggcaat
    41401 acccggcagg gtcaaaacca cccccagcaa agccatgatc cccagcacca ggaccgaata
    41461 caacaccatg gaaaaatccg caaccaatcc cggcaggcga taatatccca gcataaacag
    41521 cagaatcgcc agcagaccca tcaagccagc ctttatacta aagtccagtg aatcgctgcc
    41581 cagactcggt cccaccgttc tcttttccat aatgctgaag gaaaccggca gggcaccgga
    41641 acgcagcaat accgccagat cctgggcttc cttggcagta aagttaccgc tgatctgagc
    41701 ctggccgttg ggaataggct cgttgaccgt cggcgcactg atggttttgc cgtccagtac
    41761 gatggcaatc ggcttgccaa cattggcggc ggtcgcttcc gcaaacagct tggcaccggt
    41821 tttatcaaat tccaggttaa ccatggcctc cccgccggat gagctcaact gagcctgggc
    41881 gtccttgagg ttttcgccgg tgaccaatac cttgccggtc tcatcccaga attccaattg
    41941 ggcagtattc ttgatgatat cgacagccgc ttccgggtcg tccacccctg ccagttcaat
    42001 gacgatccgg tcattgcctt gcggatagat gctaggctcg ctgaccccca acgaattgac
    42061 gcgttcttcc aacacaccaa tcgatttttg aatggtatcc gcgcttactt ttttgccttc
    42121 tttctcctgt gcctgcagta ccacgtgcag tcctccctgc aggtccagtc ccaatttaat
    42181 gttgttcatg atcggctgat aggtaaagta agccaaaaat gccaatgcga ctatgattgc
    42241 accaataatt gcgatactgg tatttttttg caacagtttg tcctccctcc gcgttctcgt
    42301 catttattgc aagcctactc cataaacagg aaaaccgcta acctacagga gtttggcacc
    42361 attaataatt aaggcaaatt cacaacctaa atagttggct gcccgccgac acatcagcat
    42421 gcggcttaaa aagatttcaa aatactccat caccggacaa atccccgtat caatggttaa
    42481 ttccatactg atgaggtttt tctccgcttc gatcaacaga aaggaatgtt ccaccgcata
    42541 gttgacccga tcatgaatat caaacgtagc tacatcaata tttctgaccc gattgcgatg
    42601 cacatgggac ttgtcggcca gaatcagggc tgctgctacg gggttgaccg gcaatccgct
    42661 gccttcatca tgattgccga tggcagcaat gataatggca atttcatcag gatgcatgcg
    42721 catggcctgc aagatttcca gggccatcaa ggcgccgctc tgactgtgcc cgttgcggtt
    42781 gaccacattg cccaggtcgt gcatgtaacc agcaatccct gccaaatcag cggtacgggc
    42841 atcgtagccc agctgcagca aaatactgcg gctcaaacgt gataccaagg taatatgaag
    42901 cttgccgtga tctgtatatc ccatggcccc caaatgttca tttcccttgc gaataaaggt
    42961 attgatgacc ggactctttt ggatatcctc cagggttacc atgactagtc ctcaatttta
    43021 tgggaaatcg cttttttcac aaattcgatt tcggtattgt cagatacttt tacgacgatg
    43081 ctgtcttctt ttaccttgct gatttctccg cggattccgc cgatggttac gattttatcg
    43141 cccctcttca gcgattccag gatcgaatca tgcttttttt cctgcttttt gcggggcata
    43201 atgatgaaca aatagaagat caacagaaac acgccaaaat agatcgctac cgtcaaccag
    43261 ccctgctggg tatttgttgc agctgcagcc ataatattca aatctatccc tccttaatta
    43321 aatttccata actttatgtt ttttcgccaa accttgctta aatcctgccg ttataatttg
    43381 taattttcat agaattgctg gtaaaacagg ttaaaagtat tgctgtcaat ggcttcgcgg
    43441 atatcctgca ttaattttag caaaaaataa atattatgat agctcagcag aatatgggca
    43501 agaatctctt ccgccttgac cagatgacgc agataagcgc ggctgtaatt tttacagacc
    43561 tgacaattgc aattcgggtc caacggggta aaatcttccg cgtatgtggc attgcgcacc
    43621 accaccttgc ccctccggct gaaggccgtg ccgtggcggg caatacgggt cggcaggaca
    43681 caatcgaaca tatccacccc ccgcttgacg ccttccagta aatcttccgg tgcgcctacc
    43741 cccatcagat aacgcggttt gttcttggga agcaaatcct cggtcaaatc gagaatccga
    43801 tacatgtccg ctttgggctc gcccacactc agaccgccga tggcatagcc gggaaaatcc
    43861 agcgcgacga tctctgccgc actcttttcc cgcaaatcag caaaaatatt gccctggaca
    43921 atgccaaaca aagcctggtc ggagcggcta tgattttccc gacagcgtct ggcccacaag
    43981 gtggtccggt ccactgcctg ggcagcctct tcataactgc agggaaaggg ggcacactca
    44041 tcaaaacaca tggcgatatc tgcccccaac ttttgctcga tatccatgac aatttccgga
    44101 cttagaaagt gacgtgaacc gtcaatatgg gagtgaaaga gcaccccttc gtcactaatc
    44161 cggcgcaatc ccttgagact gaacacctga aacccgccgc tgtcggtcag aatcccgcgc
    44221 ggccagttca taaagcggtg cagtcccccc gccttttcca ccagtccagc cccggggcgc
    44281 aaatataaat gataagtatt ggataaaaca atacccgcgc caacttctgt cagcatctcc
    44341 ggggtcatgg ccttgaccgt cgcctgggtc ccaaccggca taaaaatcgg ggtcgcaaat
    44401 accccgtggg aagttgtcag ctgtccggta cgcgctgcac tgccggaatc cctccggatg
    44461 atttgaaaat ccagcataaa acaattcctt ccttggctca aatgatcagc atggcatcgc
    44521 cgtagctgaa aaagcggtaa ccttcctgca ccgcatgccg atatgcctgc aacgttggtt
    44581 ccagaccggc aaacgccgcg accagcataa tcagggatga gcccggcaag tgaaaattgg
    44641 tgataatccg atccactgcc tggtattgat aacccgggta gatgaatttg ttggtccggc
    44701 cagactgacc gccaaaccca tagtccttat tatataccgt ttccagggtt cttactaccg
    44761 ttgttcccac cgcaacaata tttttcccgt tacgacgggt attatttaac aattgtgcgg
    44821 tatcttcatc tatctcgaaa tattcataat gcatctgatg ctctctgatg tcggcacagc
    44881 tcaccggacg gaaggttccc agtcctacat gcaaaaggac ttgcgcaata ttaacgccca
    44941 tgtcccgaag ttgcttgagc aactcttccg taaaatgcaa tccggcagtg ggggctgcgg
    45001 cagatccttc cgcttgggca tataccgtct ggtaggtggt tttgtcttcc tctgtagccg
    45061 atcgattgat gtagggcggc aggggcatgc tccccgcctg gtcgagaaag gccatgacat
    45121 cgggacaatt aagaaaacgc aacaatcgtc ctccgttttc ccgtacagcg gttacttcga
    45181 tttccacccc ggaatgatcc ggaaagtaca cccgactgcc catgggcatg cgccgggccg
    45241 gtttgaccaa ggcttcccag tattctccct gctggttgag caacaggatt tcaatcctgg
    45301 cgccggtcga tttgcaggca aacagacgcg ccgggatcac tttggtctgg ttgataacca
    45361 gtgtgtcccc cggcgaaaaa aaatcgatga tatcggtaaa gaggcgatcc tccagcttgc
    45421 cggcggtccg atccatgacc agcaggcgag ccgaatcacg gggctcaaca ggatactggg
    45481 cgatcagata cggcggtaaa tcaaacatat aacgctgcaa gttatagagc ggcgaaaggt
    45541 cttgacggtt cacttggttt ttctcctgta acgttccatt tcactgtaca aacagccgca
    45601 atactgctga cggtacaatc ccatttccct ggcgttgata ccggtttgac gaaagccttc
    45661 ccgaaaatca cgatataaaa aaggcacttc atatttttca ccgatcaact ggcccagttc
    45721 ctgaatccag tcatggcgct gatatttact gaccagcaag gtggtggtga aactgtcaaa
    45781 ttttcctttg cgagcgatct gagcggcttt ttccagcctc agctggtaac agtaaaagca
    45841 gcgccgcgat tcccggaaac tgatggtctg aaagtattgg gccgggtcat agtcttccgc
    45901 aaacagcatg ggcagattga cttcttcagc atagctctgc aatgaatccc gacgtttctg
    45961 ccactccgta taagggtgga tattgggatt aaaataatag cccatgacct caaaaccctc
    46021 ttcgcgcagc gccggaaccg gataactggc gcaggggccg cagcagatat gtaacagtat
    46081 ttttgccatt gtttcacttc ctaaacggtg gttggataca ggaccgcaca ctatcaatcg
    46141 tcgcgatccg catcctcaaa caagctcgca gcctgaaatc ctttttgcat cggatatccc
    46201 atgacgcgat aagccgactc tgtagcaacc cgcccccggg gagtcttgtg gataagtccc
    46261 agcttgagca aatacggctc gtaaacatcg gtcagagtgt cgccttcttc ggaaaccgct
    46321 gccgcaagcg tttccaatcc caccggtccg cccccgaatt ttattattat agcatcaaga
    46381 atcatcctgt caaccgtatc cagaccgtgt tcgtcgattt ccagcatctg cagcgcctgg
    46441 ctggcagttg tctgatcgat gttgccctgg gctttgacca gcgcataatc ccgtacccgt
    46501 ttcagcaagc gattggcaac acgcggcgtg cctcgggaac agcgagcaat ttccgctgcc
    46561 ccgtccgcgg tgatggcgat gcctaatatg gaagcagccc gggtgatgat ggtggccagt
    46621 tcatgctgat tgtaaaaatc caaacgacag ctgattccga atcgatcccg cagcggcgag
    46681 ctcagcaagc ccggccgggt ggtcgcgccg attaacgtaa aaggcggcag atcgatttgt
    46741 aaagtacggg ctgccggtcc cttgccaatt acaatatcaa tggtatagtc ctccatggcc
    46801 ggatacatga tttcctcaat gctgcggtgc agtcgatgaa tctcgtcaat aaacaatacc
    46861 tcgccgggct ccagattggt cagaatggct gccaggtcac cgggacgttc aatcgccggc
    46921 cccgaagtct tgcggatggg ctgccccatt tcattggcca tgatggtggc cagggtggtt
    46981 ttgcccaaac ccggcggtcc gctcagtaaa acatgatcca aactttcccg ccgctcccgg
    47041 gcggcagcga taaacacttc caaattttgc ttggcccgct cctgaccgac ataatcggcc
    47101 atgctcaaag ggcgcagaga cccttcctgt aaatcttctt catttaattg ggaagatatc
    47161 aatcgacggt cagacatgcc agtactccct tctatctttt catcagcagg gcttgtcgtt
    47221 ttaatatctt cttgatatta tcctccaccc gtggtgaaag ctctccgctg gattgaatat
    47281 tagtcagtat tggataaatc tcgctgcggc tgtagcccag gctttccatg gcttgcaaga
    47341 tctcctcggc ggcgttggcg ttttctcctc ccaaggaaac tgttccctgg taattgccaa
    47401 gtttgtcccg cagttcaaaa atcaaacgct gggctgtttt tttgccaatt cccggaacgc
    47461 ctaccaggac tttttcatca ccgctgatga tagctgaaca cagttgttca ccgtccagtt
    47521 tacccagcaa attcattgct gcccgagctc caataccgct tacgcccatc agcagtttaa
    47581 acagttccag ctcaccccgg ctggcaaagc catacaattt aaactcgttt tccataacct
    47641 gcagaaaggt atgaaccatc acctgcccgc ccggtgccgg cagctgcgct gccatgcgcc
    47701 cgtgaatctg gacttcatat cccaacccac ccgtctcgat tacaacggcc tcggacatca
    47761 tctgaaacaa cgtgcctttt aaaaaagcga tcatcgtccc actccattct ttattcttcc
    47821 catgcgccgg gaatgcagat gacaaatggc aattgccagt gcatcagcgg catcatccgg
    47881 tttgggcaat tcctccagtt tgagaatatt ctgcaccatc atctgtactt gttttttttc
    47941 cgcattgccg tagccgcaaa ccgcctgctt gacctgcaac ggcgtatact ccgccagttc
    48001 gagcccgcgc tgggctgcgg ccatcagcag cacacctcga ctttgggcta ccgttatgac
    48061 agttttggca tttttatgat gaaagagctg ttcaatggcc acggcctccg gccgataggt
    48121 atccaggatc tgattgagct cctggaatat tttgagcagg cgcagttcca tggctatgcc
    48181 ggctgaggtt ttaatcgtcc cgtaggcaat cattttttcc cggctgcgta catactcgac
    48241 caggccatat ccggttgtgg ccgtaccggg atccaaacct aaaaccagca tgaaactccc
    48301 ctgctttctt atgaattaca tttatcttac tgcaaaaaca aactgctgtc atgaaaaaat
    48361 agaaaatcag ttcgcaaaac caccctggat accgccacac cctgccagcg tttaagatat
    48421 aacggcaact cgcatggcca caaaaaaccc gtcccggata cgaatccggg gcgggaatgg
    48481 attcactgcc ttgtttttat tgcggtaccc agcctttgtg ttaatattcg tccaggttct
    48541 ccagcgcatc attcagggag gattcattat tgaattcata tcgtcccttc atgatatctt
    48601 gccggatctg ctccggcaag cgcgcaaaaa caatcccggt aactttcaga agcgtatcgt
    48661 gttcactgtc tggtccctga taaaccgata ctcgatcctg gaacaccttg aagcgcaact
    48721 tttgcttgtc ttctgccatc cagtcatcga ttgtttgatg aatgagcaat gttttgccat
    48781 cccacgaaac ctggaatccc tggtcggcag gataaatttt tttgacatcg gttaatgttt
    48841 tgccgagaat ttcttctctt tctggaaatt cagatatttg cattttctgg gagcgaacat
    48901 attccttttc ccacaccaca gcagtgtcgc cttgtacccg acgttcccaa ccggcggtct
    48961 gaaccgtcgg tttttgcgca attggccatt tttgttggtt tcgaatcaaa ccggaaagtg
    49021 tatagcctgc aacagagctg gccagcaaag tcaggatgag aatgataccc agcttggttt
    49081 ttcccatgtg tattcacctc ttaccggcat tatctccatt ttttgggctt ctatacccga
    49141 gcatcaccgc ggagacaggg taacatgaat aaaaattaat tttcgacaat atatttactt
    49201 tgaaggtcat aaggtataat atcttcttaa agagtatata atatagtaac aattttgtca
    49261 taattttgat attatttcga atattcggac aaaatatttt aaggaggtga aacgtatgac
    49321 aaacatcgac gccagagtag aaacaatttg cagcgaactc aaccacttga gccatggatt
    49381 gatggacatt taccagagct acaatgatgc cccggcttat caatcgacgt ttgcacccga
    49441 gaagtttgca gttaatcagg aaaacctgga tgaatttgat tggtctggaa tgcagtatga
    49501 tttcaatgtt gacttgttcg aaactgcccc caaggaaatc aactgggatg acgtaaatgc
    49561 ttccattgat gaagcgattg ccagcggtca tttgattgca cctgcggcga ttgactatgc
    49621 ccccaaacgt tcctatggcc acaatgtgct gcgcagtgcc ggtacctatt acaagggtga
    49681 catccgcgac atggacttta tgctgcggca attaaaacgg ctggaggcca atcccgctga
    49741 cattttttct ctcgatgaca gtgcttttga cagaagctac aacatggtga gcaacttcga
    49801 gatgaaggtt ccgctggaga tttacggaca aggcgaagaa ctggtgatca aggctgatat
    49861 cccggagatt ctgttcaaca gcgccaaggc cgaagatctg aacacactgc cctttaaaag
    49921 cgtattggcc ttgttacccg aaagaatcaa tacccggcag ctcaagccaa cctttgtgga
    49981 tggagaattc cgtctggaac ttcccaagcg cgaagaaaaa agagtccgta aagtttttta
    50041 atctgactgg ccagcgccaa attgaatcgt accttgtcag gcataaaaag cccgctcatc
    50101 agtttactcc aatagcgcac attgatgagc gggcttttgc ttgtttatga agatcccttt
    50161 attaatgcca aatctacatc agtttttcca tcagttcatc ggcaatggaa aaattactgt
    50221 agaggttttg aacatcatca tggtcctcga gggtatcgac cagcttgagg attttttgcg
    50281 ctgtagcctc gtcggcgatt tccacggtcg tctgcggcaa catgacgata tccgcgtcct
    50341 caatccggat ttctttttcc agacggtccc ggacttccat aaaggcgtcg ggcgaagtaa
    50401 tgatttccag tgtatcatcg gcttcgcgca catcgtcggc accatattca atggccatca
    50461 gcatgaactc ttcttcatct gttttgatat cttcccggtt gatcacgatc aagcccaccc
    50521 gatcgaacaa ataagccacg caaccgcttt caccaaggtt tccgccatat ttggcaaaca
    50581 gatggcgaat atctgaagca gtgcggttgc ggttgtcggt ggcgatgtcc agcaagatag
    50641 ccgctccgcc cgctgtatag ccctcatatt tgatttcttc aatggcatca ctttcaattt
    50701 ccccagttcc tcttttgatg gcccggctga tgttgtcatt gggcatgttg acggccttgg
    50761 ccttctggat ggccagccgg agcttggagt tggcttccgg gtcgccgccg cccgaacggg
    50821 ctgccaggat tatttccttg gcaattcgtg tgtattcctt gccttttttt tcatcggtac
    50881 gggctttctt gtttttaatg gtcgaccact tgttgtgtcc ggacatcaaa gttcctcctt
    50941 cattttgatt gtgtctacca gatcccggga atcatttttt gggtcgtacg catgtacgcc
    51001 tgatactcgt cgccaaaatg gtgcaacata aattcttctt ccagattgac tctgcgaatc
    51061 aacgctggaa tggttgtcag cacaatgatc agcagggcgc cccaggaatt aaaaatcatc
    51121 ggaatggcaa gaaaactgag cagcgaaccc aggtataaag gatgtctgat cctccggtag
    51181 gcaccttccg tgatcagttg atgcttcgag tacacggcaa tacgggcatt gaaataacgt
    51241 ccgatggaac ggaaccccca ccagcgtacc aggcaggaaa cgacaaacaa gaccagaccc
    51301 acatagataa tcgtcggttc ccaatcaaac cggcgggtga catgccattc aacaaaatca
    51361 attgtagcaa ataacaaagc caacatgaaa gatgcttgca ggtacaaata gcttttctgg
    51421 tcatcgtcct caatgacaat atcgccaggg tcttgataaa tccagatttc cgaaatcagc
    51481 tgccagacca gataaagcac cacaaaacca accatgacgg taaaatcaaa atgaggcagg
    51541 accggccagg cgagatagcc aacataggca aagatagcca aggcagatat gattctgatc
    51601 aacacattcc tttgccgact gttcatgtta tgcttccccc attccggccg gcaaaacagc
    51661 gatcggcggc acactgcgct tatgggcgct acgctcgtac atggtctgaa ttttaccgac
    51721 cagttccgta gctcccgcgc cggtgcggat atattcgtcc agttcccgat aggtaaatcc
    51781 catttcacct tcatcggtct gtccccccca caatccagca gaaggggctt tggtgatcag
    51841 ctgttcgggg atattgagat agcgggccag cgcatagacc tgttccttgc gcagatcacc
    51901 cagcacctgt aaatcgactc cgttgtcgcc gtatttggta gcatagccga cggtaatctc
    51961 acttttgttg ctggtgccca taaccagata ttgatgagcc tgggccatat aatacaatga
    52021 ggtcattctg agccggggtt tgacattggc ccgtaccagt cggccgctgt cttccggcag
    52081 gtcaatggca tgggccagtt ctttcagcaa agcgcggtag gcttcctcca ggttgatgat
    52141 atgatagtgc atgtccaggc tctcgaccag cagcagtccg tcctgtaaat cctccgcact
    52201 gctttcacag ggcatgagca aacccagaca atcatcggga aaagcctgtt tggcgagagc
    52261 tgctgccaca gccgagtcaa tccctccgct gatgcccagg attaagcccc ggcaacccgc
    52321 agcctgtact ttttcacgta accagttgac caaataatcg cttacggctt ctgtgttcat
    52381 agtgctttcc tccttattaa aagattggat tggtcgagga atagtttgct gcaccgtcat
    52441 cgccgttgat atcgtccagt attcctgctc ggccttcttc cagtccggtc gggcctgcaa
    52501 tgccaggcct ttgtccaggc tggtcaacat cgacaagcct gccaaccggg tcatttacat
    52561 attcaacagg acccgaacaa atcctgcctc cccatctcat tttctctata tgccgcacgc
    52621 gttccgcaat ggtgcgcaaa caatcaggac aaacgctcgg attgtctaca gcctgacgat
    52681 ttcgagttcg ccccaggctg ccattttctc ccccttgatg gccagaatgc cttgcacccc
    52741 ttttatattt tgcgcatatt taatcgcttt cagcaaatcc tcttcatcct gcaccagatt
    52801 ggcggctccc gtggcaactg catcagccag cgcggtatca ggcgccagaa tcactaccgc
    52861 atcggctttt cccagactga cactggggcc gaccgtcccg gaagaggtac agatccccag
    52921 cggcgtgctg tccgccttga ttttcagtcc gatccgctgg ctgaagcggg atggtccggc
    52981 gaacaccgct acgatacgat ccctttgact gttgaggtag atgtctccgc cgttttccac
    53041 gatggtttct ttgacttcgc gcatcagccg ttcccccacc gcctgggcaa aagcacccgc
    53101 caccgctgcc atcggcccta cgccggcctg cacggccgct tctgccattc gtctggcgat
    53161 ctcaggtgcg tccggcaaca gcgccagtgg gacaaaggaa gtgcggaaag ccggctgtcg
    53221 gctgagatat ccttccagct cggcgcgcag tcgttttact tcctttacac acaaagcgat
    53281 caggtgctct gtatcgtgca cctgatctat ggcaacggcc aaatcggttt ctttgacctg
    53341 aatatgaaaa taacggaggt cacgggcttc atgcagggca cggtagtacc tttgcacata
    53401 ttgttctccc gtttttctgt ccatgactcc tcctatacgg cctctttgat ttcaaaatgc
    53461 aaaatgatgg ctcgcgtcgg gcagcattcc agacacattc cgcaaacgac acaccgttca
    53521 ttatggaagg atacttccat ggtatcccgg tcgatcgcca gcgcctcggt tgggcaaaaa
    53581 gaagcacagg ctccgcactg gatacagatt tgttcgttcc agatgatgct ttcgctcagc
    53641 ggttccagga caacgcccag actttccaga aaacgtgtac ccgcttcgta gttttccttg
    53701 ctgccttcca gttccaccac caggtatcct tctttttgcg gattgatatc agctttgagg
    53761 atattgataa tcaaatcata attctttacc aaacgataga taatcggctg ctcggtttga
    53821 gcggtggaaa aatagcaaac cagtttgttt ttcattattt attcctccca ttcgccagcc
    53881 cgttgggggt tctctccggt atggatttaa atacaatatc tgcctcggca gaaggaattg
    53941 gagccaccgg ccgggtgatt tcaaacttgc cttgcaggat ccactgtttg agtatttctg
    54001 cgatcttcct ggcccgggga taactggatt gcggagtggt gaccaccgac ttgccgttga
    54061 tggtaattcg tccgctcttc agctctttaa aattggcaaa ccccagatcc atcggtttgg
    54121 aatagggata cccttcatta tagtccacga ttggacagta tatgtcttca tcttttacgg
    54181 cggtataatg cagcatctct tcattcaaaa tgggaatagg gatgccgata cccaccgcca
    54241 tggtcgctcc gtagccaatg tagctcagac ccaccagcca gtcggggtcc atgcccttta
    54301 aatcgccgat tacggaaagg gttccggcgg tcaccttggg cacaccattg tcaccgcgca
    54361 gcacattggg atgatgctgg gttccgttcc aggcgacata gccgacgccg ccgcccagga
    54421 aaatgcgcgt cccgatgccg atggtgcggt aatgcgggtc gttgagcagc ggactcagtt
    54481 gcccggcagt ggaataactg gcattgccga cccggggttt taagatgccc atatacgtat
    54541 acagtgtgcg gtcgccgaga ttgacagcac aattgtaatt ctggtaagca ttgcggggat
    54601 tgaataaaaa ggcgtcattt acatcatcca gagttataac ggtatccacc ttgcggttgg
    54661 gatagcaatc cgtaccgtag gcggtggctt ccagacgaat ctccttgccg gccaccagat
    54721 catggatgac atgcccgccg ccgtagacga aacgccccgg aaacaccttg ttttccggat
    54781 cgttttctgc tacctgggca gcgccgatat acgcatcgac agccgcaatc cctgaatagg
    54841 cctcgactcc gttcagccag actttcttca ttttgatgcg cggattgcta tggccaaagt
    54901 tgataaaggc accggaggaa cacatcggac caaacgtacc ggtggtcact acgtcgattt
    54961 cccgggcagc ttgctcatag cctttttctt cgacgatacc gatgacctct tccgcggtga
    55021 caacaaccgc ttcgcccttt ttgatttttt cattaatttc ttcgtaactc ttttgcactg
    55081 ccattttgtt tcttcctccc gtgattattt ggtcaacaaa cccatcagcc gttgggccgt
    55141 cagtacggcg gcccgggcgt cttcggaata cgcgtcggcg ccgattttat cagcatattc
    55201 ctggtttaaa acggcaccac ccaccatgac ccgggacttc aggcccctgg cctggacccc
    55261 ggcgatcacc tcctgcatgc gcggcatggt ggtcgtcata agcgcgctta acccaatgat
    55321 atcagcattt tccagagccg ctcgttccag aatgacatca gcttttacat ctttacccag
    55381 gtcaatgact ttaaatccgt agttctccag caaaactgcc acaatatttt taccgatgtc
    55441 atgaatatcg ccttctaccg tggccagcac tactacaccg gcatgcccgc cgccatcccg
    55501 ggacagcaat ggctgtagaa tagcaaaggc ccgtttcatg gtttctgcag cggtcatcaa
    55561 ttgaggtaaa aagtagcgtt tctgatcgta cagctcaccg gcggcttcaa tgccgggaat
    55621 catggcctga ttgacgattt ccagtggctc cagcttgctc tggagcagcg cctcatcaat
    55681 caatgccgta atgttttcct tgtctccctg caaaaccgct ttgcggatcc gaccctgaat
    55741 ggtattgtct tcacaggctg gcgccgctgg caaagttgct gtcggagatg cctgggccgg
    55801 ttcactgccg ctttgcagta aaaccgggat attgcagctg ctgcaaaccg cgtgacgcaa
    55861 ggttgttgca gtcgcggaag cagctttgta atccttgtat ttattaatat acgcagcgct
    55921 gtttacatcc ttgcccagca gcaccgctgc tgcccggcag gtatccatca tacgctcgtc
    55981 aaaaggattc agaatgggca gatccagccc ggcactccag gccatggcca gataagcgga
    56041 atttaaaatc tcccgggccg gcaatccgtg ggaaacattg ctgaccccca gaacggtggc
    56101 aagtcccagt tgctccttaa tcatctgcag cgccttgatg gtttccataa cctgatcctg
    56161 ctgggcgctg gcggttttaa ccaaggtatc aatcaataca tcctgtgggc gcagcccgta
    56221 ttccagcgaa cgctgcaaaa tttttcgggc tacggccact cgatcttcag cccggtcggg
    56281 aatgccctgt tccccaatgc atagtcccag taccgctgcg ccgtatttcc tggccagtgg
    56341 cagaatcttt tccaggtttt tatcttctcc agtggtggaa ttgatcaacg gcctacccac
    56401 aaacatcttt aacccggctt ccacgacctc aggatcggtg gaatcaagcg aaatgggaat
    56461 atccagcgcc gcctgaatca gactcactgc cttttgcatg gctcccacct catcaatgcc
    56521 gggtacaccc atgttgacat ccagtactgg ggcaccggct tcctgttgct ggcgggcttc
    56581 cgctaccacc acctgcatct gtccatcttt gatgtcctgg gccagttttt tgcgggcagt
    56641 aggattgatg cgttccccaa tgaaagccag ggggtgcttg tcgcccagca aaacgcaccg
    56701 gctgcgcgac gccaaagcag tcgcggccgc ctgactgcgc gcggcaggtt tcatcccccg
    56761 caacactctg gccattgact gaatgtggtt cggggtggta ccacaacagc ctccgatcaa
    56821 attggccccg gcttccttta aacgcaatgc atattcggcc atctcttccg gcgtatccgg
    56881 gaaaaccgtc cttccgtcca ccagtcgggg aagcccggcg tttggctcca cggaaagcca
    56941 ggtagaaccg taccgcgcca agcgctggat gaccggcagc agttcccggg ctcctcccga
    57001 gcagttggcg ccgatggcca aaggctgcag ggcttccagg ataacgaccg cggttagcgg
    57061 atcggttccg gtcatggtcc gttcgtccgc ttcaaaactc atatgggcaa tgaccggcag
    57121 ccgggtgttc tcacgagcgg caatcagagc tgcccgcatc tcaccaatgt cggtcatggt
    57181 ctcaatcgat attaggtccg ccccggcatg ggcacaggcc aacacctgtt ctttaaaaac
    57241 cgcatacagg tcatcaaact ccacctctcc catgggccgc agcagttttc cggtagggcc
    57301 gatggaagct gcaaccaaac ctcgttgtcc aacggcacgg cgggcaatcg acaccagctc
    57361 ggtgttgatc tctgccacac ggttttccag cccgtattcc gccagcttgt atcgactgcc
    57421 gccgaaggta ttggtttcaa tgatgtccgc gcctgcttcc aggtattgac gatgaatgtc
    57481 cgccagaatc tccgggtgct ccgcgccgaa cagttccggg caatctccgg gctgcatgcc
    57541 gcgcgactgc aagagtgtcc ccatggcgcc atccaggatc acaacgcgtt ctatcagcaa
    57601 actctgtaaa tccacctaaa atcactcctt catacaaaaa agccctctac aagagggctc
    57661 atcatgctgc tccctctcat ctcccaagca tacgcctgca ggatttggca ccattcccta
    57721 tgggcggttg ccgggtttca tcgggccagt ccctccacca ctctggataa gagtataaat
    57781 attttctgga ttttgatgtg ttattttagc attaatttgc ggtcttgtca tcaggaattt
    57841 tatcctgttc cgcttccggt agtaccgccg acttctctcc aacttcagtg gcatctggtt
    57901 catcgctttc atcagcaatg acttccgatg ctacaattat ttcttcctca accggtttgg
    57961 cttccaaggg attgggcggg atgtccttgg gcgcaggttt ggttaaatca tccaagttga
    58021 ccgcatcctg aaactctttg cgcacaccgg tcgtgacttt gcgaaattca ttcaagcctt
    58081 taccaattga gcgggcaaca tcgggcagtt tgcccgggcc aactacaatc aatgcaatca
    58141 acaggatcaa caccagttcc caagggccaa tgttgccaag cataacatcc tcctgtatat
    58201 cttttcatgg tttttataaa tagttttcat ttattgtact ctgattaacc ctgtaatagc
    58261 aattaatttt gcacccaacg cactttttat ggccacagct atcttttatg gcaggatttt
    58321 acataataac tgcgaatact tatggtaata agatttttca ggagataacg atgcttgcta
    58381 ccaccaacac tttattaata aatggtatcg aggcggttcc ggtcaaggta gaggtagaca
    58441 tacagaatgg attgcccaat tttgaattga tcgggctggc ccaggcagct ctgaaagaag
    58501 cccgggaaag ggtcaaagct gccttgaaaa actccggcta tgagtttccc aatcgcaaaa
    58561 tcatcgtcaa cctggccccg gccgatttga aaaaggaagg cagccatttt gacctggcaa
    58621 ttgccctggg catcctgctg gcctccggac aaatagaggc aacttttatt gaaaacctgt
    58681 tcttcgcagg ggagctctcc ctggacggca gtctgcgcca ggtaccgggc atcctgccca
    58741 tgagtctggc gctgatcgga gcaacacccg acacacgttt catcgtacct gctgccaact
    58801 gccgcgaggc tggtctggtg catgagaccc ataccctggc cgcagccagt ctgcagcagg
    58861 tgattgactg gctggaaggc aacgatacgc tggatctggc cactgaagaa tatgggaaag
    58921 aaaatcgaag cggcgaaccc cagccggact ttgccgaagt caaagggcag gaatccgcca
    58981 agcgagcgtt gcaggtcgcc gcagcagggc tgcacaatgt cctgctgatc ggccccccgg
    59041 ggggaggcaa aaccatgctg gcccgtcgca tgcccagcat catgccggat atgacccggg
    59101 aggaaatttt ggaaaccacc aaaatttatt ccgtcgccgg tttgttgact gcagatcatc
    59161 ctctgattgc ggtccgtccc ttccgcgcac cccacaaaaa cgcctctacc gccagtatta
    59221 tcggcggcgg caaagtaccg cgcccgggcg agatcagtct ggcccaaaac ggtgttcttt
    59281 tcctcgacga atttactgaa ttccaccggg atgtgctgga agccctgcgc cagccgctgg
    59341 aagacaaact ggttaccatc gcccgggccc aggccacttg tacatatccg gccaacttca
    59401 gtctggtggc cagctgcaat ccctgcccgt gcggttttta tggctctgat ctggagtgca
    59461 cctgtacccc gctgcaaatc caaaaatatt tgggtcgcat tagtgggccg ttgctggatc
    59521 gcatggatct gcatgtggaa gtgccgcgcg tcatctttga acaattgcgt accgacagca
    59581 acagcgaaaa ctctgcctcc ttgcgggaaa aggtactgct cgcccgggaa atccagttgc
    59641 agcgctttgc ccggcacaaa ttcaaactca attcgcaaat gcgtcccact gacgttaaaa
    59701 agttttgccg tctggacgaa gcctcagaag ctctcttaaa aaatgccttc gaccggctaa
    59761 aaatgagtgc ccgtgcctat gaccgcattc tcaaagtggc ccgtaccatt gccgatttgg
    59821 ataacgttgc gaatataaga atccagcatc tggccgaagc catgcaatac cgcagtctgg
    59881 atcgcaaata ctggcataat taagaccagc cgggagaaat atatttagtc atgtcaaggg
    59941 gccggcattg tctaaaatgg tgcccgccca ttcctatgac aaaatttctg aatatcttcc
    60001 gtcggtgaca cttcttgaac acttctttca tttcatcaac aacaattata taatttaagg
    60061 aacagaatac gaaatgcaat actgtaccct tcgaaacggt cgagcatctg caggaaagga
    60121 gcaagcagtg agttacaaaa aactcggaaa gagaattcag catgccaggg aggaggccgg
    60181 tttgagtcag gaacagctgg cagcgatact tggttgttcc cagccaaccc tttccaatta
    60241 tgaaaaagga aaaagcagga tttatctgac ccaattgcaa aaagtttcag agatattaaa
    60301 ccgcccggtg caatattttc tggaagcaat ggaaccttca gaagaagagc gaggccaagc
    60361 gtatgcagcg gagattagcc gttcttatct ggaagaagct tcctcacaaa tggacaaaga
    60421 catcttatcc attttgaaaa tattgcagga gttgtccccc gaagcccgca gaaccgtcta
    60481 tgagtttgcc caatgggaat atcataaatc tcggaggtat ggtaacaatg gttaatttcg
    60541 cactcaacaa caatcagcaa agccttatca aaagcattcg tctgctggcc ccccagctgg
    60601 cgcaaaaaga aatggaaatt gatcagcacc gcagtgaggc atttgattgc tcactggtga
    60661 atgaactggc caagctaaat ctgctgaacc ccatggttcc ccctgaattt ggcggacgcg
    60721 gcctgaacta cttcgattac gctctgctga tggaagagat cggtgccgtc agccccggcc
    60781 tggcagcggt catgatcttt aactgtcatt ttatcagcat tctggatagc atcggcaccg
    60841 aagaacaaaa agctcaatac ctgccggtat tcacccagca gcaacccaag ctggccgcca
    60901 tggcactcag cgagaaaaat gccggctccg atgccggcaa catgagcact ctggccgtga
    60961 agaaaaacga tcactatctc atcagcggca ttaaagaata cgtaaccaac ggtacgaatg
    61021 cagattacat cgtctgtttt gccacgctcg atcccacccg tgcccgttcg caaatgctgg
    61081 ccttgcttgt cccgggcaat gcaccgggga tccgccaggg gcaattgcgt aagaccatgg
    61141 gcatccgcta tgcccactgt acggaactga tttttgacaa cgtttccgtt cccgcagcca
    61201 atcttctggg cgaggaaggc aacggataca tgatcatcac ccaggcgatc gatcgcgaca
    61261 aggtgctgac tggtgccatt ggagtcggca tcgcccgcgc agcttttgac atggccctgg
    61321 cctttgccaa ggatagaaaa caattcggcc gcacgattta cgaaaatcaa gccattgcct
    61381 ttgaactgtc aaagatggcc gcccagatcg aagccgcccg catggtcgtc tggaaagcct
    61441 cctggctgct cgataacaat gaagatttta ccgtcgccgc atccattgcc aaaattaccg
    61501 gcactactac cgctgaagaa gtagtcagca aggccatgga tattgtgggc gggcgggcct
    61561 atctggaagg acatccactg gagaagctct atcgggatgt caagattttc agcaacctgg
    61621 aaggcaccaa ccacattcaa aacgcgatta ttacttcgct gttgtaggca gcgtggggat
    61681 ttatcccgcc ggcaaccaac acaataatga attggggaga gtttttatgg acgcatatgt
    61741 cttgtccatc gatcaaggca cgaccggcac caccattctc atcatcgatc acgaaggacg
    61801 gatcgtagcc cgggccggcc gcgaattcaa gcaatattac ccccagcccg gctgggtcga
    61861 acatgacccc cgggaaatcc ttgaaatcac ccgcatgctg atccgcctgg ccatttccca
    61921 agccggactc agcgccggtc agatcaaagc catcggcatt accaaccagc gtgaaaccac
    61981 ggtcgtttgg gatcaataca gcgggcagcc aatcggcaac gccatcgtct ggcaatgccg
    62041 ccgcactgct cccctctgcg aagaaatgaa ggccgccggc tggacggaga aaatcaaggc
    62101 ccgcaccggc ttgatcatcg acgcctattt ttcagccacc aaactggcct ggatgttgga
    62161 aaacattcct gacgcccgta aaaaagcgga agacggccgg ttgctgttcg gcaccatcga
    62221 tagctggctg ttgtggaatc tgagcggcgg tcgggtacac gccaccgatt atacgaatgc
    62281 ctcgcgcacc atgctgtatg acattcacca gctaggctgg gatgaagaga tcctggctta
    62341 tttgaacatt cccccaacca tgctgccggc ggtacgctcc tgcagtgaag tttacggact
    62401 atgccaggca gatttgttgg gagaagaaat ccccctggct ggcgtggccg gcgatcaaca
    62461 agccgccctc ttcggtcagc actgctttac gcccggttcc gccaaaaaca cctatggcac
    62521 cggctgtttt ttattgatga acaccggctg ccaggcagta acctccgccc atggtctggt
    62581 gactaccatt gcctggggac tcaatcaaga aatcacctat gcgctggaag gcagcgtttt
    62641 tgtggccggg gcggctgtgc aatggctgcg cgacgaaatg cagctgattg ccaattccgc
    62701 tgaaagtgag gccgtcgccc gctcgctgga gagcaatgac ggcgtctaca tggtgcctgc
    62761 ctttgtcggc ctgggcgcgc cctactggga catgtacgcg cgcggtacgc tggtagggct
    62821 gagccgcggc accggacgcc ttcacatcgt ccgggcggta ctggagtcca tcgcctatca
    62881 aagctgtgat gtgctgaccg ctatgatgac cgatgccaac atccgcctcg attcactcaa
    62941 agttgatggc ggtgcctgca gcaacgactt tctaatgcaa tttcaggcag atatgctggg
    63001 cgtcaccgtg gaacgtcccg ccatgatcga atcaactgct ctgggtgcag gttatttggc
    63061 cggattggca accggcttct ggtcgaatct ggaggaactg cagcagactc gcaaggttga
    63121 ccgtcttttc catccccgga tggaggcagc ccaaagacaa atcatgctaa acggctggca
    63181 gaaagccgtt aaatgtgccc tgaccacccc gggaaaagcc gaataacgac gttgttttcc
    63241 atgaccaatc gtttttttaa ttgagatata gtccttgggc aaaaagagga gggagcagaa
    63301 gaatatgtat gaagattgtg tatttctgga tttcgattat atggaaaaat tcatggcgga
    63361 agtcttttgc ggacttggcg tcccccgcga agacgctgaa atctgcgcgg atgtactgat
    63421 cacctccgac aagcgcggca tcgattccca cggcatcggg cgcctcaaac ccatttatta
    63481 tgatcgcatc aaagccggta ttcttgaccc cgtgacccgc atggagatta tacgggaagg
    63541 accaactact gcggtgatcg acggtcacaa cggcatgggc catgtaattg gccagcgcgc
    63601 catgcaaatg gccatcgaca aagccaaaaa attcggcatg ggcatggtcg ctgtccgcaa
    63661 ctcctcccat tacgggatcg ccggttattt cgctctgatg gcagtacaaa acggcatgat
    63721 cggaatcacc ggcaccaacg cacgcccttc catcgccccc actttcgggg tggaaaacat
    63781 gttgggtacc aatcccatga ctttcggcat ccccacggat gaacccttcc cctttgtact
    63841 cgactgtgcc acctccgtat cccagcgtgg caagatcgag gtttatgacc gggaaaacaa
    63901 agaaattccg gaaggctggg tcattgacaa gagcggtcaa acccgcaccg acacccatca
    63961 aatcctgctc gacctggtca gcgggcaagc cgccctcacc ccgctgggcg gcatcggcga
    64021 agacaatggc ggctataaag gctacggcta tgcaaccgtg gtggaaatct tgtccgctgc
    64081 cctgcaaggc ggaactttca tgaaagccct gagtggacgc gacgcgaacg gccaactgcg
    64141 tccctacaat ctcggccact tcttcatcgc catcgatatt gaagccttta cagagctgga
    64201 cagtttccaa aaaagcaccg gcgacatcct gcgcgccctg cgtgcttccg agaaagcccc
    64261 cggcgccagc cgcatttata ccgccggtga aaaagaatat ttggcctggc aggagcgcca
    64321 gggcaaaggc gtgccgctga acaaagtgct gcagcaggaa gtactgcaaa tgcgggaaga
    64381 gctgggactg aaagatttcc attttccatt tgaataaatt tcataaatgt tctgcagcta
    64441 aaaccccgtt tttacggggt tttatatttt ttaatggaca gaaatgtttc acgtgaaaca
    64501 ttgttgccag tgttacatat tacacgagga cgttctttct gtaacattgc tgactctctt
    64561 ttatattctc aattccagta attcttctat tgaccgcagg tcatcccctt tcatggacct
    64621 taatcacatc cttgtcccgg agtaacacta cagccagact gggctgtagc tattcttttc
    64681 tatggttagc tgggtcaaga gccgcgcatc gattgttttg gcatagatat cgcgatggac
    64741 gaccggaatt ggataaaagg tggcggggtt gctcaacacc aggagaaaag cctggtccgc
    64801 gggggaaaag ttgtcattgt tgaggctgac ctccttggca taaatttcct cgccttccag
    64861 cgctacttcg cggtagtaac ggttgacctt cagataaaac caggggagga attctccaaa
    64921 ataatcccgc tgcaggagca ggaagtagtc gcgagtcctg gaccattggc cgaagaagtc
    64981 attacggctg atttcgatta gatcctggtt ggtcaggtca tacaggaagg tgaaggtgac
    65041 tctgcccgga tcggcgaagg ccataatcag cactttattg atctcggcag ccaaggcaaa
    65101 aaaccagata ctgtagtccg gcaaggccag cacctcttgg tcgttgccgg tttcagtgct
    65161 gattttccgc aaggcgctga cgccgtcgct atacagcaga ctttgattat ccgtaaacca
    65221 ctggggcatc atgccgctaa atgaaaaaag cgttctgcca tcctgggtgc tgattatccg
    65281 gccattgtcc aaggccagtt tgctgccgtc ataggaccag gcgatggatt cagcaggttc
    65341 agaaagattc agactgcggg aggtgccttc ggtcaggtca tggatgatga ttcggtggcc
    65401 gtcgtcggcc aacagagcaa ctgctttccg gtcccggcgc agaaatagtt ccctgcggct
    65461 gagattggca aatctcgcca ggcgggtggc gcttccgctg gcaaggctta gttttatcaa
    65521 atcgtcgctg ctgtcagtcg gctgcaggaa agcgatttct ccctgcagtt tggcattata
    65581 accgggcagc acaatggtct cagcgggaat caggaaatcg tccgggacaa ggttgttggc
    65641 ttgttgtaat tcacctaacg gcaaatcgta tttctcagct acttttttca gggtttcgcc
    65701 ctgctcggcc agatgaaagc taagcggcgg cgggatcagc aacagctggc cggtcttgat
    65761 ggtgtaatcg ggggcgacca aatggttgag gagaataatc gcttcgcttt tctgtttgaa
    65821 caagcgggag atggtaaaaa ccgagtcgcc ggcctgtacc cggtagtaat attcctgctg
    65881 ggggaaggcg atgctggcgc ccagcgccag ataggtttga gcgccgacga tgccgtctac
    65941 gacaatggcg gcggcggttt gaaaatcttt gacggcggca agggtggctg gctgaaaata
    66001 gccgttgatt tccccacggt aaatgtttag ctgataaagg taatactgca gccagtaaac
    66061 gtccagtccc tggtcgttgc tgcgcagcgt tcttccaccc aggggagcat ggatgaatat
    66121 tttacggcag gtgtcggggc cggcctggcc atcgctcacc agatcaggaa agtcggcctg
    66181 caacatctgc accgccccct cggtatataa cccaaaccgt ccgtcagcgc ggcgattgag
    66241 ataggctttt ttatatgctg tcaggcggtt ttgcaaaatc gtcacatctg caccgcggga
    66301 tccgtttttt aaggttcgcg atgaaaagac tttttcgccc gttgcataaa cgccggtcct
    66361 gtgccccaga cgcagaaatg tcttgggccc cacgatcccg tccctgacca ggccgaaata
    66421 cttctggaag ttcctgaccg cggtccgggt aagcggaccg aataccccgt ccaccagcag
    66481 cttgcccggt acaatctggt caggcaactg gttgagcagc gcctgaagga tttgcacatc
    66541 cgttccctgc aactgcggcg atgtcaaacg caggttgcgg ctgccaaaaa aagaatatcc
    66601 ctgttcattc atcttcatat ctccttctgt gaagtcaata caatgtatga aggataggga
    66661 tggtttataa ctatgccccc ttggctggac ttgtccgctt ggtcagctga cgatctggtt
    66721 tttgcctctc tttttagctg catagagctt catgtcggca gcatggatca gctcggatag
    66781 ctcttccccg tggtactgct ggactccgcc actgatggtt atggtgaggc cgtcatcaaa
    66841 gaggttgctc tccacggcct gccggatccg ctcggcgact cttgccgctg cggtcaagtc
    66901 agcattggga aagatgacca tgaactcttc tccgccgtat cttccaggaa aatcgctgtc
    66961 tcggacgctt tccttgatga gcgcggcaat tttcatcagc accttgtcac catagatatg
    67021 ccctttgctg tcattgactt gcttgaaatt gtcaatgtca aacaaggcta ccgacagggg
    67081 cttgtccaga cggttggcat caacgatctt cgcttttaaa tgctcgatca gcgtccggtg
    67141 gttgccgatt tgggtcaaac cgtctatctc cgcctgttcg gccaggatct tgtttttttg
    67201 ttcgagctcg atctgcatcg ctttctttgc ggttatatca aagacaatgc cggctagaaa
    67261 tacgggcttg ccgttctcat catattgggt gatgcggccg cgatcataat accatttgta
    67321 gttcccgtct ttggcttgga tccgatattc aacctcatat acgccagccc gtccgtacag
    67381 atggttgcgc atgacatcca ttgcaagggg taaatcctcg gggtgaatcc gctcggtaaa
    67441 aaactgataa ggaaccggtt ggggcatttc tgccgggtca tagcccagtg tggtaatctt
    67501 gagcgggtta aaggttaccg cattggtcct gatattccaa taccaatggc ccagatttcc
    67561 agtccaggca tactccagcc tggtttcctg ctctttttca tgcagcaatt gtcgattcaa
    67621 gatctccagt tcttcaatcc tttccagcag ctgttctttg gtgaaatcgc tgtagtccat
    67681 gacgccaccc tccttatctg ttctctttgc ccacatttat actgccactg ttttcccttc
    67741 cacctgctgg attacccaag agcgcactgt ttccagcact ttttcccgat cgggggagag
    67801 ttcattcatg gtttcatgat atagtccgtc aaagaggaag agccgtttat cctgcgaaga
    67861 ggccttttgc atgacctgtc ggctgccctg cgggtcaacg acggcatccg cagagccgtg
    67921 gatgatcagc agcggcaggg tcagttcacc ggctcgctgc aggcattctt gcccggcgcg
    67981 ggtgaattcc atgtaccagc gggcgctgac caggtcatga accagcggat catggacata
    68041 atcatcaact acctttaggt catgggacaa ggcgctgctg tcaaggccag tcggcatgct
    68101 cagaccgggt atgatgttgg ccagaagggt agccatggtt tttttccaga tcggcacctt
    68161 gaccgcctgg atgagaccgg cggaggaaag aaccagtccg tcgatgattc cgggataatc
    68221 cagggcaaac ttgcaggcaa tcgtcccgcc catgctgtgc cccagtagaa tcaacggcag
    68281 gccgggcaac ttatctctga ccagcttgca aaaggttttc agatcggcgg tataatcgtt
    68341 aaacgagttg atgtgccctc tttttccctc cgaacgcccg tgaccacgat gatccaaagc
    68401 ataaaatgag atcccgtcat cttgcatgtg gtcgatcagg ttgcggaaac ggctgccatg
    68461 ttctccaatc ccatgggcaa tcaccactac cccgcgggga cactcggcct gccagctctg
    68521 ataaaagata cgggtgtttt ccagaccatt gaagttgccg gtatttttaa tgtattcagc
    68581 caccctgttt actccccctt ttccgcttgc gattcgttct cttctgcaat aaacgtggct
    68641 tcttctcttt gagtatacca ttcacggtct gctgtatcat caagcatttt ttgatgcaga
    68701 ttgccttgtc gaaaatttca taaacaatct agcggatgat tgcataagtc cattttctgg
    68761 tttacaataa accaaattgg aggtgttgcc agtcatggaa cctgaattta aaactattgc
    68821 tgaatcggtt gtcagtctca gtcaaatcat cctgccggcc cagaccaatc ccgctgggac
    68881 cgctcatggg ggcgagctgt tgaaaatgat ggataatgcg gcgggagcgt gtgccaccaa
    68941 acactcgcgt gccatcgtag tgaccgccgg cattgacaac atcacctttc atgcgccggt
    69001 ttttatgggc aacctgacca tctgcagtgc aaatctcacc tatgtatcca accgcagcat
    69061 ggagattgcg gtcaacattg aggcggaaga ccttttggcc ggacatcgcc aatgctgtat
    69121 gaccgcttat tttatttttg tggcactgga tgaaaaaacc atgacgccaa aagcgattcc
    69181 tcagttgcag ctgctcaatg aaaccgagcg acaggagttc gaggcaggac ggctgcgaac
    69241 gcagacgcgc aaagaaagtc ccaaggcttg ttggctgccg ttgtattaag taccgcgatt
    69301 gcatccaacc tccttacaat tgaattccat gcaaacaagg aaccggctga ttcaagaact
    69361 ttaaagcgtt cggaatggcc ggttttttat gctgtcagac aggtgttgca ggcctgtgtg
    69421 gttgcctgta acgatattgt ccaatcctgg attttattct ctgtgactgt tccccggtct
    69481 tgttgctgct gcctgccaac cgcattggaa tttcggcgat aggaccacct ttgctgctca
    69541 tatttcctgc aacaaacaca gattgtcaaa cagttcggca actgttacca aaccgactcc
    69601 gccagggacc gcactggcca tgcgggcttt gcttctggcc tgctcatcta catcaccaaa
    69661 catattaccg gctgcatcaa aattaatccc ggcatcgatg ataatggttt ctttcctgat
    69721 catttccgcc ttgataaaat tcagttgccc catggcagcc accacgatgt cagctctttt
    69781 aatctcttca gccaaatcac gggtacgggt atggcacacc gtgacggtgg cattttccgc
    69841 catcatcatc agcgccagag gacttcccaa tatgctgctt cgccctacga tggtaacccg
    69901 ggcgccttcc agatctacgt tgtgatcctt cagcattctg atgacggctt tgggtgtcgc
    69961 cggtcttatc ccattttccc ggttgataat cttgcctaaa ttgaaattat gaattccttc
    70021 cacatctttg tcagggttca tggcattgac aatgacctca tcccgcagct ggggaggaaa
    70081 aggcttctga agcataatcc cgttatatct ttcatcctgg ttgagttgct tgagctcaat
    70141 cagcaacttt tcttcagaaa tatcttcaga aaatttgatt actgccacat caacgcctaa
    70201 ttcgttgcca aatcttttaa taccgttgat gtaggcatct gcatctggct gttctcctac
    70261 ctgtaccact gccatggcaa tttttctgct gccgatacga ttgattaatt gctgctttaa
    70321 ttgcgcccgg atttcgtgcc cgtaaatcag catagccgtc ctcctgtttt cgtttataat
    70381 taaaaccagc ttgtcctgag attccgcggc gttgctccag tatacaacac ggcaacaaac
    70441 caaggaaact ctatcaggaa agttttcaca agtaaaacgc ttacacaaac attattatac
    70501 ccaatttgcg gccaaaccac gttagctatt ataacttatc cggtcgtggc cgcaagcttg
    70561 atttgcgttc ctgtccgccc tttaaaaagc cgctttgata tgattaatgt ccggttggga
    70621 ggaccgcatc ataatagtca ctacatcaaa gcgggtctcg cggtgcttcg caggctgatc
    70681 gcccaggtag tgcaaggcgg ctttttttat atgttcgatt ttggttcggg taatggcttc
    70741 ttcgggagaa ccgaacaagt cactgcggcg ggtcttgact tccacaaata ccagcaagcc
    70801 gcctttcctg cagatgagat ctagttctcc atagcggcaa cggtagttgc gggtcagtat
    70861 ttcataaccc tgctgctgca ggtagg
//
LOCUS       DAOTHZ010000003        70090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_344_length_70090_cov_8.458842, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000003 DAOTHZ010000000
VERSION     DAOTHZ010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 70090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70090
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_70090_cov_8.458842"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1380)
                     /locus_tag="P5549_00755"
     CDS             complement(13..1380)
                     /locus_tag="P5549_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW11635.1"
                     /translation="MGTYMDEYRSKLISAEKAASLVASNSIIDYGMFATKPVDFDVAL
                     SKRVGDGLENVSLRGTGTVLPVPEVIKNDPQQQTFQYFSWYYTLIDRTAGDYGLCCHN
                     PFNYHEATMLAANTDFSHIWPSVWCAQVTPMDSSGCFNFGLGNSHNRGLALASKIAIV
                     EINENMPRCLGGCDEYVHISEIDYIIEGSNTPIFSTPPSAQPTPEERKIAEYVVEEVV
                     DGAVIQLGIGALPNLIGHMIADSDLKHLGVQSEMFCDAYVAMYEAGKITNAKKARDLY
                     KSTYAFCLGTQETYDFLNDNPRCASCNVYYTNEPSLIAMNDNYISINNILEIDLLSQV
                     CSETSGLRQISGTGGQLDFVVGAFHSKGGKAFLAFSSTYKDKEGKMHSRIKPLLTPGG
                     VVTVPRTQVQWLVTEYGKVNLKALSIWGRADALVSIAHPDFRDDLIKSAKEMKLWTRT
                     NRIPF"
     gene            1696..2784
                     /locus_tag="P5549_00760"
     CDS             1696..2784
                     /locus_tag="P5549_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRW11636.1"
                     /translation="MYTTRIITIDEKERYNDFVRQHPKGHFLQSWEWGQVKRGMGWMP
                     LPLILEKDGEIEATLLILKRALPLPGLNKSIFYSPRGPVMALDDPALAWELFAGVRAV
                     ARQHGAIFLKIDPDVPVSDQVFADILKECGFARNETGLDFEGVQPAFVFRLDITPSEE
                     QLLSNMHAKTRYNIRLADKKGVQIRKAKGKKDLRVFYDVLLETAERDRFLIRGYEYFA
                     MIWEHMVENDLGEIFLAEWEGKVVAATLVMCMGDKAWYLYGASSNQYRNVMPNYLIQW
                     SMIRWAREKGCTLYDFRGVSGDMDESNPLYGLYKFKKGFNGQFTEFVGDWDTVYDKFF
                     YFLWTKALPVYRKSTRLLLAKRKEAEAD"
     gene            2784..3980
                     /gene="alr"
                     /locus_tag="P5549_00765"
     CDS             2784..3980
                     /gene="alr"
                     /locus_tag="P5549_00765"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRW11637.1"
                     /translation="MQPNLADKWIEINLDAIENNLHAIKTRLAEGVRLIAVIKADAYG
                     HGAIATAQLLRELGVTCFAVSFFQEAMQLRQAGVTDSILVFSPIVSTEELKDAIQNNL
                     TITVASPYDWQLLNQASALLDERVRVHLKVDTGLGRFGLREKEIMELLPEIKRNQRIN
                     IEGIYTHMAAAAASVSYTRRQFDDFLHLIDKLEQAGLRVPLRHCANSAVLLKYPQMQM
                     DAVRVGTLLSGQIPVGVAATGLQLEDPYAFKTRIISLRQHNKGDYLAYYRSYRLKKAA
                     KIAVIPVGFRDGLALQVGNKPSGWLDLIKTAVKLILNYLNITRFGQQVSIKGKNYPVR
                     GKVFMQMALVEIPLDQEVGIGDEVVVPVRKTLADPAVARIYWREEKVVKFTTKEGGSE
                     ALVELL"
     gene            4075..4446
                     /locus_tag="P5549_00770"
     CDS             4075..4446
                     /locus_tag="P5549_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW11638.1"
                     /translation="MKGKILIVDDQKGVRRLLEELFKKEGWDVKVASDGQEAISLVKE
                     KLPDIILMDVKMPNMNGLEASQVILQAHSQIPIIMMTAYGEIEVVMKALEAGVRKCIT
                     KPFDIMVLRDMVNHLMHERTA"
     gene            4581..5225
                     /gene="fsa"
                     /locus_tag="P5549_00775"
     CDS             4581..5225
                     /gene="fsa"
                     /locus_tag="P5549_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRW11639.1"
                     /translation="MRIFIDSANIEEVRDIETMGILAGVTTNPSLVAKEKRDYRDVIR
                     EICAIVDGPISAEVIAQDYAGMMQEAQMLAAIHPNVVIKIAMTEDGLRAIRDLRRKGI
                     STNATLIFSATQALLAARAGASYVSPFLGRVDDTANDGLILLEDIMIIFDQFDLDTEV
                     IAASIRHPVHVLQAARTGSHIATVPYKIIKQMIKHPLTEAGIQRFLQDWQEAFQ"
     gene            5294..6280
                     /gene="glpX"
                     /locus_tag="P5549_00780"
     CDS             5294..6280
                     /gene="glpX"
                     /locus_tag="P5549_00780"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRW11640.1"
                     /translation="MERELALEFARVTEAAALASARWVGLGDKEAADDAAVTAMRVMF
                     DTVSIDGVVVIGEGEMDEAPMLYIGERVGLRVAPQVDIAVDPLEGTNLVAKGLNGAIA
                     VLASAPMGTLLHAPDMYMDKIAVGADCKGRVFLDAPVKENLRQVAKALDKSIREVTVV
                     ILDRERHQGIIDEVRSVGARIRLISDGDVSPGVAAAYGNSGVDILMGIGGAPEGVITA
                     AAMKCMGGDFQARLFPEDENEIRRCHEMGIKDVNQLFTIDELVKSDDVIFVATAITDS
                     YLLKGVQYAKGRAITQTLVMRSASGTIRFIEANHSLDKKPLFKDKRISLTLE"
     gene            6576..7874
                     /gene="rho"
                     /locus_tag="P5549_00785"
     CDS             6576..7874
                     /gene="rho"
                     /locus_tag="P5549_00785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRW11641.1"
                     /translation="MKLNISELENKTMLELYQIAKEINLAGYSKLRKKELVFEITKAL
                     THSNELLQASGVLEIMPDGYGFLRPFAYVPSHEDIYVSPSQIRKFDLRTGDRVGGQVR
                     PPKDSERFFALLKVEDVNSLPPEESGKRIHFDALTPLYPTQRLTLATDSAELSTRIID
                     LVAPIGKGQRGLIVAPPKAGKTMLLQKMAQGITTNHPEVELIILLVDERPEEVTDMQR
                     STQAEVVASTFDEPPENHVKIADMVLERAKRLVEHKRDVVILLDSITRLARAHNLVIP
                     PSGRTLSGGIDPASLHKPKRFFGAARNIEEGGSLTIIATALVETGSRMDDVIFEEFKG
                     TGNMELVLDRKLSERRIFPAIDLKRSGTRKEELLFNADEMELMWNLRNTFSEYNSVET
                     MQMMIDTMKKTKSNDDLLRAAPIIFGKRESPRRNGSKYYE"
     gene            7989..8795
                     /locus_tag="P5549_00790"
     CDS             7989..8795
                     /locus_tag="P5549_00790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRW11642.1"
                     /translation="MTVCIHPANARLIEGKSNLSKTNGEKTKTAVLYHIEAGDSLWSI
                     ARRNGVDLGNLMTVNGLHEKSVLAIGQVIKIPTGSSKFHTVQAGDTMWSIAAHYGLPV
                     AALEQANPDKKPQCLNIGDRLTVPQRSSGQLLQASETSRGISFPGLFAWPLAGVITSH
                     FGWRNSGYHHGIDIANRLGTPIKAAADGVVTFAGWKSVYGRTVIIKHNNGQSTLYAHT
                     QKILVKEQQRVKKGQVIARVGVSGKTTGPHLHFEIRQGDKCINPLRYLQQ"
     gene            8958..10211
                     /locus_tag="P5549_00795"
     CDS             8958..10211
                     /locus_tag="P5549_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW11643.1"
                     /translation="MFLTLYANQKGEAMENTEVIMLGRSGSEWVEVAPDEMIPLPKGA
                     SLVLLPGFVPIGLDRQTGDLQYFTCLPDDKREPATAVAALLPQGFTRTLLPACVSPEA
                     QPLPLLGYAAVGFKDNQIWVAAVKSDEHRKWHPHYYNTAALPQRIDRMLRKYPDNRIL
                     RQLARCSLQYSCYTAQNLFYGRWEAGIPTMAACNADCIGCISESHLEVESPQHRIDFL
                     PDVQEVSEIGLEHLLHAREAIISFGQGCEGEPSLNADRLAESIRSIRRETDRGTINMN
                     SNAGFTQGIIKMTDAGLDALRVTIFSCQEKNYNIYHRPRNYRLEDVKTSIQYAHNHGL
                     RVSLNLLTFPGFTDREDEIEALLQLMAQGHVDMLQLRNLNIDPEELFCRFESGGEILG
                     IANFIDIIHQEMPQVQIGSYTHPSR"
     gene            10258..11253
                     /locus_tag="P5549_00800"
     CDS             10258..11253
                     /locus_tag="P5549_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRW11644.1"
                     /translation="MENNKKLMNVREMQASSVGQQVSGKFLILDKIYRKTKDGKDMYN
                     LKLGDGTGEIDAVVWENCRLTGTLTSGEVIGILGDMGNFSGRNQITAKIIKVLDEDPG
                     LYMKQAENLEQLKQHFQELVKSLRDPHLQNLSRRIFDPSTQEVFFRAPAAKRIHHNYP
                     GGLLEHSCQVADLALKMADSYAYLNKDLLLFGALVHDIGKIMEYKMGASPQYTTEGKL
                     LGHIVMGSEMISIEINRLRTEGYDFPQNLEWMIKHMILSHHGSMEFGSPVIPLFPEAF
                     VLHVADNLDAKMYIFKNKLQENNGEEEYFTNYDTYFAQQFFTYRYPVWDEMKKTE"
     gene            11417..11620
                     /gene="rpmE"
                     /locus_tag="P5549_00805"
     CDS             11417..11620
                     /gene="rpmE"
                     /locus_tag="P5549_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRW11645.1"
                     /translation="MKEKIHPKYGITTARCACGNEMEIGSTKENIKVEICSKCHPFYT
                     GDTRRIVDTTGRVERFKKKYGLT"
     gene            11763..12830
                     /gene="prfA"
                     /locus_tag="P5549_00810"
     CDS             11763..12830
                     /gene="prfA"
                     /locus_tag="P5549_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRW11646.1"
                     /translation="MLEKLASLELKYNELTDQLSNPEVIADQSKFQKYAKAHAELTDV
                     VISYQEYKKACQELEESQLMLREESDPEFKEMLTAEIQALQEKIAAAEGQLKVLLLPR
                     DPNDEKSVIMEIRAGTGGEEAALFAGDLFRMYSRYAEEQGWRIEIMDSHYTELGGIKE
                     LIFVVDGVAAFSKLKYESGVHRVQRIPSTESGGRIHTSAATVAVLPEAEEVDVAINEN
                     ELKIDVYCASGPGGQCVNTTQSAVRITHMPTGLVVTCQDEKSQHKNKAQALRVLRARL
                     QEMMEAEKNEEMAGARKSQVGSGDRSERIRTYNYPQGRVSDHRINLTLHKLDQIMNGQ
                     LDEIIEALMANDQAERLKKVD"
     gene            12831..13700
                     /gene="prmC"
                     /locus_tag="P5549_00815"
     CDS             12831..13700
                     /gene="prmC"
                     /locus_tag="P5549_00815"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRW11647.1"
                     /translation="MQAVWRIKDLLQWTTRYFLDRGIKESRLEAEILLAHVLKKDRVY
                     LYANYEAPVNPDERALYKEYIKRRTKSEPIAYITGHKEFMSLDLIVTPDVLIPRPETE
                     LLVETAIKAAKDLEKVRIVDVGTGSGAIAVSLAYYLKQAQVSATDISLPALKVACQNA
                     ERHQVSIDFHAGSLLESFFTRPAGEPSAHFDILVANLPYVPETDRDSLEPQVRDFEPH
                     LALFAGEDGLDLYRQLLPQARRVLPEGAIMLLEIDPRQAGLIASSMQGFSEIRIIHDA
                     AGRARMVQARRSQ"
     gene            13697..14674
                     /locus_tag="P5549_00820"
     CDS             13697..14674
                     /locus_tag="P5549_00820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRW11648.1"
                     /translation="MIATRYLKVDAQHPDPERISQAAQLICQGELVAFPTETVYGLGA
                     AALQADAAAKIFAAKGRPAEHPLLVHISQMEQVKMLVTEIPPEARRLMEHFWPGPLSI
                     ILPAREEVPEIVRGGSSGVGLRMPSHPVAIALINAAGPLAAPSANLYGRPSPTQADHV
                     RTDLDGRIAAVLDAGETGSGLESTVLDLNHNFRILRRGGIPVEKIEALLGIRIGLPEP
                     AVENYNSPVQVLLNQNRSELVQRTEGLQQANKRAGLVRVSESKQDYPCRFAKEYTLKL
                     DGLGQSFFTILRDAEQEQIDILLFEPLDESNNRLSEAMLDRINRAVFRK"
     gene            14692..15282
                     /locus_tag="P5549_00825"
     CDS             14692..15282
                     /locus_tag="P5549_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HRW11649.1"
                     /translation="MYEQMGTIILVAIVLGADAFSLSMGMGIKGASRRYAIRFSLLVG
                     LFHVLMPLLGLSLGLAAGKVLGVWAGRIGAVVLVYIGLEMIWKSYRELQPRVITLAKA
                     RASLDRSEQAMSADKWPSLLILTVSVSIDALTVGFSLGTIKAPILLTVVIMGMIAGSM
                     TLLGFKGGKILSRSVGSYSQAAGGLILLALAGKMAF"
     gene            15333..15791
                     /locus_tag="P5549_00830"
     CDS             15333..15791
                     /locus_tag="P5549_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRW11650.1"
                     /translation="MHILFVCSGNTCRSPMAQALFTDHVKNTGKNRQESQPIAKSAGL
                     RALDGSPASPAAVMVMTEMGLNISNHRSRRLNTELVEWADVILTMQESHRREVMEKYN
                     VPAQKVYTLAAFSRDSAGDILDPFGFEIETYRQCARQINSMIRNIVKSFS"
     gene            15855..16310
                     /gene="rpiB"
                     /locus_tag="P5549_00835"
     CDS             15855..16310
                     /gene="rpiB"
                     /locus_tag="P5549_00835"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRW11651.1"
                     /translation="MEIAIGSDHAGVEKKEEIIALLNKSGFETIDCGCFTTASVDYPD
                     IAEAVAAQVREKNIPGIVICGTGIGISIAANKIPGIRAALCSDTFTAEMARKHNDANV
                     LAMGARVLEMPVMEAIVNTFLTTSFEGGRHQRRVDKINAIENTYCERCK"
     gene            16310..17566
                     /gene="glyA"
                     /locus_tag="P5549_00840"
     CDS             16310..17566
                     /gene="glyA"
                     /locus_tag="P5549_00840"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRW11652.1"
                     /translation="MDYIEKYVLPADPEVAAAVAAEERRQDRKLELIASENFVSRAVM
                     AAQGSVMTNKYAEGYPGKRYYGGCEYVDVVEELARERLKKIFGCDHVNVQPHAGAQAN
                     TAVYFAALSPGDKVLGMNLNHGGHLTHGSKVNISGKYYQFYEYGVNAETEMIDYEALR
                     LVALETRPKMIVAGASAYPRVIDFAKFREIADEIGALLFVDMAHIAGLVAAGLHPSPV
                     PYADFVTTTTHKTLRGPRGGVILCKEEWAKKIDSAVFPGIQGGPLMHVIAAKAVCFQE
                     ALQPAFKQYQQQIVDNARVLAQELIAHGLRLVSGGTDNHLMLVDVRPKKLTGKEAETI
                     LESINITVNKNGIPFDPEKPTITSGIRIGTPALTSRGFSTEDMQAVAHAISLALDHPE
                     DEAKLGEAREIVQVLCDKYPLYGISL"
     gene            17563..18051
                     /locus_tag="P5549_00845"
     CDS             17563..18051
                     /locus_tag="P5549_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRW11653.1"
                     /translation="MSKQSSILRKRPDWDQYFLELADLVASRSTCLRRQVGAVLVRKE
                     RIISTGYNGAPRGLGHCLELGCLREEMQIPSGQRYELCRGVHAEQNAIINAAFYGVST
                     QDSVIYCTNQPCLICARMIINAGIIKVVHRGNFDDQLAVQFLQEAGIELVEIPPTIER
                     KS"
     gene            18053..18364
                     /locus_tag="P5549_00850"
     CDS             18053..18364
                     /locus_tag="P5549_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HRW11654.1"
                     /translation="MDTKTLSLPRLNGLSPTLESTALKLMEEAGELAQCIGKFRGLSG
                     ETVHMEESHVIEEIARELLDVAQTAITMMFVLEDHYQIDIEQMLQNHVEKLDRKGYLS
                     L"
     gene            18688..18939
                     /locus_tag="P5549_00855"
     CDS             18688..18939
                     /locus_tag="P5549_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRW11655.1"
                     /translation="MADKKQNWPRALADAMNLATSVAAAIAVGYFGGKWLDGKFDTGP
                     WLSIGGLLFGMLTAGKMMWERLMIESRQKPSPGSAEDQD"
     gene            19043..19726
                     /gene="atpB"
                     /locus_tag="P5549_00860"
     CDS             19043..19726
                     /gene="atpB"
                     /locus_tag="P5549_00860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRW11656.1"
                     /translation="MIFGMWDGVYFHIGPIPVDHLVVTQWGIVAVIGLLAFFATKNMQ
                     VCPKGLQNAFEWIVVWTTDFFTQLMDKEHIAKKYAPLLSTFFLFILFSNYSGLIPGAA
                     HIQGFQPPTSNWNVTVTLALITVFAVQSAGFAEHGFHYLGHFVSPNPLMLPLNLLEEI
                     THPLSLTVRLFGNIFGEEMIIAYMMFMVPFLIPSALMALSVLLGAIQAIVFTILSASY
                     IAAATGEGH"
     gene            19787..20005
                     /gene="atpE"
                     /locus_tag="P5549_00865"
     CDS             19787..20005
                     /gene="atpE"
                     /locus_tag="P5549_00865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRW11657.1"
                     /translation="MGIALGAGLAVSIAGLGGGVGMGVAGGKAIEAIARQPEVGGDVR
                     TLMFITFAFIETLTIYGLLIAFMLVGKM"
     gene            20125..20745
                     /gene="atpF"
                     /locus_tag="P5549_00870"
     CDS             20125..20745
                     /gene="atpF"
                     /locus_tag="P5549_00870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRW11658.1"
                     /translation="MKRNKREYLFGLLLAFVMVLGYATLCMASGDASVPVAAPEGKPP
                     VFGNLYVIGWSAVNFFVLLALLYKFGFNPVNAMLEQRANTIESAIKHAEEIRVEVDQL
                     RKEAQANLAESRKEAQEIVARATKAADDAKTEIAAQAKEEAAAMKTKAQAEIASATEA
                     AKAELRNTAATLAMMAAEKVLGRAITEEDHKKMVKEFVDEAGDFLC"
     gene            20739..21287
                     /gene="atpH"
                     /locus_tag="P5549_00875"
     CDS             20739..21287
                     /gene="atpH"
                     /locus_tag="P5549_00875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRW11659.1"
                     /translation="MLNKSVARRYAEAFFGIAREADKIDAYQAELEKVISTIAEVDGM
                     KEYLDHLLVPAREKKDLVQKVFGTQLSPMTMNFLFMIIDKRRESYLEFIYREYVEMAD
                     ESRNIKKAELFAAREVPQQELDALEERLSASTGKKVMLKLTVDPTLLGGAKIRMGDQI
                     VDGTAVKKLQMLTEKLKKAKIS"
     assembly_gap    21330..21429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21465..22979
                     /gene="atpA"
                     /locus_tag="P5549_00880"
     CDS             21465..22979
                     /gene="atpA"
                     /locus_tag="P5549_00880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRW11660.1"
                     /translation="MSIRPEEISAILKEQIERYQSEVEVSNVGSVIYVGDGIARVYGL
                     QGAMAGELLEFTGGTFGMVLNLEEDNVGAVLLGPYYHIKEGDIVKATGRIMEVPTGNA
                     MLGRVVNALGQPIDGKGPINTDKFRPVERVAPGVVTRAPVNVPMQTGLKAIDSMVPIG
                     RGQRELIIGDRQVGKTAIAIDAIINQRTKKDMICIYVGIGQKQSTIAGIASKLEEMGA
                     MDYTIIVAATASEPAPLLYIAPYAGVAMAEEFMESEKADVLIIYDDLSKHAVAYREMS
                     LLLRRPPGREAYPGDVFYLHSRLLERACKLNENYGGGSITALPIIETQAGDVSAYIPT
                     NVISITDGQIYLETDLFYAGQRPAINAGLSVSRVGGSAQTKAMKSVAGQLRLDLAQFR
                     ELAAFAQFGSDLDKATLARLTRGERVTEVLKQGQYVPMATEEQVIMIFAGVNGYLDDM
                     PKNKVQEFETDFLKFMRSTYADVLATIRDAGKLDDATQDKLVKGIQEFKSTFAV"
     gene            22993..23877
                     /gene="atpG"
                     /locus_tag="P5549_00885"
     CDS             22993..23877
                     /gene="atpG"
                     /locus_tag="P5549_00885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRW11661.1"
                     /translation="MAGAGVREFKRRIRSVKNTQQITKAMKMVAAAKLRKAQEQAEQS
                     RPYTETLQGTLARLASLATDVQHPLLVTRDEVRKVGYIVITADRGLCGGYNTNIIKVA
                     NDAMATDERKVENAVIAVGRKARDFYRKRKGLEAEYTNLGDTISYTDAREIAEYIINA
                     YENEELDEVYLVYARFVNVLRQIPTVAKLLPIEKLDAAEETVEKYVEYIYEPSAELIL
                     LTLLPRYVGSQIYHAMLEGKASEHGARMTAMGNATQNAGEIIDSLTLQMNKVRQAAIT
                     DEILDIVGGAEALNKGGK"
     gene            23881..25311
                     /gene="atpD"
                     /locus_tag="P5549_00890"
     CDS             23881..25311
                     /gene="atpD"
                     /locus_tag="P5549_00890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRW11662.1"
                     /translation="MANVTYGEVTQVIGAVVDVRFKPGELPDLMNAITIKSEDQDSSA
                     RVIADLDVTLEAMQLLGNDTVRCVALSPTDGIMRGMKATNTAAAIKIPVGDGCLGRIL
                     NVLGREVDNAGPVNASEYWEIHRNPPPLTDQLPATTMLETGIKVADLICPYAKGGKIG
                     LFGGAGVGKTVIIQELIRNIAYEHGGYSVFTGVGERSREGNDLWGEMTESGVIDKCAL
                     IFGQMNEPPGARLRVALTGLTVAEYFRDVGGQDVLIFIDNIFRFAQAGNEVSALLGRM
                     PSAVGYQPTLATDMGLLQERITSTRKGSITSAQAVYVPADDLTDPAPATTFAHLDATT
                     TLSRAISELGIYPAVDPLDSTSRILDPQIVGPKHYNAARGVQQILQRYKELQDIIAIL
                     GMDELSDEDKLIVARARKIQRYLSQPFHVAEQFTGRPGIYVPVAETVESFTQVLAGKH
                     DALSEEAFYMMGNIDTVLANAKRLEG"
     gene            25315..25734
                     /locus_tag="P5549_00895"
     CDS             25315..25734
                     /locus_tag="P5549_00895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HRW11663.1"
                     /translation="MADNTFMVEIVTPEQILFKDEVQFLVVPELNGELGILKNHAPMI
                     AALDVGVMRYTDPAGKVKKIALSGGFMEVIYNEVRVLAETAEQGSEIDILRAKAAKER
                     AEKRLQAINENINHARAEMALRRAVTRLKAADALLKE"
     gene            25904..26128
                     /locus_tag="P5549_00900"
     CDS             25904..26128
                     /locus_tag="P5549_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11664.1"
                     /translation="MDKLMLLKALVIAITVYYLMKGILYLVLWQVLSKAEENGKVRAA
                     KARSIIMEQRRKRDEREAQLAQERKEKRLY"
     gene            26191..26421
                     /locus_tag="P5549_00905"
     CDS             26191..26421
                     /locus_tag="P5549_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11665.1"
                     /translation="MGDLQIKFLILIVLVFLITAGFCILIWQQTKKFEKKALLAKQRQ
                     KEYEKELRLKIQEEKRAQKEKAAVTSSQEPEN"
     gene            26732..27463
                     /locus_tag="P5549_00910"
     CDS             26732..27463
                     /locus_tag="P5549_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HRW11666.1"
                     /translation="MRKLFIYIFVFILLCYVIETEIKHSIAEPIDHGSPYCLSFTSIG
                     AILLESRLDCWAKIKTANHFQELDAELIMILQLLDLPFQRSAIMHEQQTDYISLFYHA
                     TKNQTDYFIRLQTESQNTYFLLTAISSQDDLALHQDEKRLKERYQSQSYFQYKGMMNE
                     RPDQAERRQMLQVVCQSLQARAIDHYSAQNMDCMAAFSPLLRENYDQVKLAGENYNLQ
                     AAIRNDSVLNQSYIYLGVPLLLNDY"
     gene            27488..28723
                     /gene="murA"
                     /locus_tag="P5549_00915"
     CDS             27488..28723
                     /gene="murA"
                     /locus_tag="P5549_00915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRW11667.1"
                     /translation="MYIQGRTRLKGEVEISTSKNAILPIIAATVLTDEKVVLKRVPRI
                     KDVDTICQILQQLGKEIQVKEDYLVFNSRVLQTTPPYDLVRQMRASFLLMGALLSRCG
                     KVRVSLPGGCAIGNRPIDLHLKGFAAMGAEIKFNGGDVIAKANKLMGNHIYLDFPSVG
                     ATENIMMAASLADGTTWIENAALEPEIVDLANFINMMGGHVSGAGTNVIKIQGVELLS
                     GTEYTPIPDRIEAGTYLIAVAATAGEAVIKNVIPEHLKSITAKLEESGAVIEESNYAI
                     QIKREGKIKPVYIKTLPYPGFPTDMQAQIMAYLSFANGTSVISESVFENRFMHVQELL
                     RMGADIRTEGKSAIIRGVKELTGARVRASDLRAGAALIIAGLSAQGKTIVTENEHVER
                     GYEDIDKKLKQMGAQIYLN"
     gene            complement(28812..29750)
                     /locus_tag="P5549_00920"
     CDS             complement(28812..29750)
                     /locus_tag="P5549_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRW11668.1"
                     /translation="MKIFYASDAHAAESTFRKFTNAGPFYKADLVIYGGDFVGKAVVP
                     IIKTKEGYYEATYYGSTVKIKNEGELPDLQKTLRDAGFYWIILSEQEIDQIDESGVER
                     ITKELQIKQIEEWAKLMDERFKKNGIPCVVIPGNDDPFYIDDYLNKMQYVQNGDDKIV
                     EIKGYEILSLSHGHPTIFKYPRDVSEEELAQKIEKLASQVKNMNKCIFNIHVPPRDTD
                     LDQDTLYDEDLNPILDGDSLATGPVGSTAVAEAIKKYQPLLSLHGHVHPSRGICKIGR
                     TICVNPGSNYDEIQLRGCLIDINNRGEVELPTLTSG"
     gene            complement(29782..31374)
                     /locus_tag="P5549_00925"
     CDS             complement(29782..31374)
                     /locus_tag="P5549_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRW11669.1"
                     /translation="MARGLELFQQSAVGFTRQISPYDTLVYSTVNPGLMYALVYLMWA
                     PFLYPGAHMSWAILTVSQMFVIAGLYWLLSVAMPRQGGEYIYISRILHPSLGLMSSFM
                     ISFTAISWTGVCSDWALKYALVEFFASMGIVSGNQMWMQWASFLNTPHIRAILGTIMI
                     FIIWMVFYKGSRAMMKMSWMALIGTGVGALTFIVAALMADPGTFAANFTRLSGLNYND
                     VIPLAVQAGHPNKFLFGATIMAGSTYIILNTLGATFGANLAGEVRSVQKSQLLAMFGS
                     LVILMSAWAIFYGLSYKIFGNLWTNALMYLSGTGNAAYPFGAYEPFATILLGILTQSP
                     LFIFLIAIAFFMATFGSCAGMSFGPTRNIYAWGFDRLVPAKTSQIDPKSGSPVIASTI
                     ALAVAEIFLLLDVYLPAWTAYIGYTIFTWFLAWILLGIAGIVFPYRRKLLFESAPPVV
                     RGRFMGLPVVSWLGILTTIISVSICAYLLIPFFKGDMPYTMIVMSIILLVLPIIIYYL
                     SKASYAKQGINIDIQFLEIPAD"
     gene            complement(31441..32562)
                     /locus_tag="P5549_00930"
     CDS             complement(31441..32562)
                     /locus_tag="P5549_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HRW11670.1"
                     /translation="MKTLGVIVNPIAGIGGKVGLKGSDGLEILKKAREMGAKSDAPRR
                     AQEALHIIAKIKNDIHVLAYPDEMGANECRQAGLEPEIIGSITSNETCPEDTMKAAQK
                     MTERGIDLILFAGGDGTARNIFSAIGSSIPVLGIPAGVKIHSAVYAINPHSAGEVASL
                     YLQGKVVNLREAEVMDIDEEAFRQGRVSARLYGYLKVPVERRVQSVKSGARSEQAEVE
                     GMAAEIIAGMDADTLYVIGPGTTTRSIMERLGLPNTLLGVDVIFDRKLIARDVNENQL
                     FDLINQHGKARIIVTAIGGQGHILGRGNQQISPRIIRLVGKDNLTVVASRDKLVALQP
                     HPLLVDTGDPSLDQELLGYVRVVTGWEDSIMYRISDDAE"
     gene            complement(32629..34191)
                     /gene="gcvPB"
                     /locus_tag="P5549_00935"
     CDS             complement(32629..34191)
                     /gene="gcvPB"
                     /locus_tag="P5549_00935"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRW11671.1"
                     /translation="MARQKKLLRDSYHQARWDEPIIFEMSVPGERGVLVPRACQDIVN
                     QVGDGVSAIPANLRRKKSPALPEVNQMRVNRHYYHLSQETLGTDVTLGISEGTCTMKY
                     SPKIQEHMAARHPGIVDVHPLQCPETMQGILETYYQVEQFLKEISGMDAFSLQPGGGA
                     HGVFANASMIRSYHRARGDKKRTEIITTMFSHPCDAATPNTAGFKVITLMPDESGYPD
                     LEAMKAALSDRTAGIMITNPEDTGIYNPRIEEYVKAAHAVGALCSYDQANANGMLGIH
                     RAREAGFDLVHFNLHKTFSAPHGSMGPATGAIGVKEFLKPYLPVPRVEFDGHKYLLNS
                     NYPESIGKVRTFFGNTNVVLKTYMWIMQHGAEGLKEAAICSVLNNQYFMKKIAEIPGV
                     VIWYAKGKRRLEQVRYSWEKLKQDTGVGTDDVNKRIVDFGIEHYWTSHHPWVVPEPFT
                     LEPCDSYNKEDLDEYVEVLRQISKEAYEDPDFVKRSPHKAASHRPTHEAIDDADLIAV
                     TWRQYLKKKAKP"
     gene            complement(34226..35632)
                     /gene="gcvPA"
                     /locus_tag="P5549_00940"
     CDS             complement(34226..35632)
                     /gene="gcvPA"
                     /locus_tag="P5549_00940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRW11672.1"
                     /translation="MHKESIVYPYIPNSVPEIKAAMMKEVGVTNPMQLYEEIPEHLKY
                     SQEMQLPQPILDEYSIRRHTEELLGKNKTCGEYLSFLGAGCAQHFVPAVCDEINGRGE
                     FLTAYVGESMGDHGKWQALFEYCSQMGELMDMDVLSIPMYDGGQAAASSLRMANRINN
                     RKKVLVPATMNPDIFMIISNYLDSVPAAELQIIPIGFDQKTGLMDLNDLKKNISNDVS
                     AVMIENPSYLGFIETQAEEIGKIARAAGAEFVVYADPISLGVIEAPANYGATITCGDY
                     HPLGLHLGAGGCQGGFIATHDDMKYIAEFKDLMFGLTDTLQAGEWGFGEVLFDRTSYG
                     SRDKGKEFTGTSTGLWAITAGVYLALMGPKGMQEVGTTIMQNCQYAMQKIATLPGVKI
                     QLAAPSFKEFVVNFDMTGKTVAQINKHLLQFKIFGGKDLSQEFPQFGQSALYCVTEIH
                     QHKDLDKLVYALQGVISC"
     gene            complement(35689..36096)
                     /locus_tag="P5549_00945"
     CDS             complement(35689..36096)
                     /locus_tag="P5549_00945"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HRW11673.1"
                     /translation="MEKTMTTCKVKSCTFHHPDDLCAAGEIQVENNRNSAICDTFYPR
                     DVNKQSVHKSSAQAGIDLRDSTLADDNLGINIADSSHLYESSASNLTPLVTCNADDCK
                     YWEHDICKAQEITIDGPTAELSADTRCQMYTPY"
     gene            36324..37160
                     /locus_tag="P5549_00950"
     CDS             36324..37160
                     /locus_tag="P5549_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRW11674.1"
                     /translation="MEISSQRFWIKNQQRMDKNELLPDFIEKGVSLYEVLRVIQGVPL
                     FLECHLARLKHSAQLTQLELPLDDHQISQRLLALINSNQVEQGNIKFVLNYPAAGQPA
                     DFYAYFVEHDYPSPEDYCQGVKTVLVQMERPNPNAKIDRADYRKRIDEAKRKTGSYEA
                     LLVDRERNVSEGGRSNLFMIKDEMVYTAPANKVLKGITRKMVLVACENRGYQVCEKEI
                     SLDEMLAMDAIFISSTSPKVLPVRQVDDNAIDSGKNKVVQEIMAEYDAIIDEYIERHK
                     AI"
     gene            complement(37226..38371)
                     /gene="grdD"
                     /locus_tag="P5549_00955"
     CDS             complement(37226..38371)
                     /gene="grdD"
                     /locus_tag="P5549_00955"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HRW11675.1"
                     /translation="MENNRNMHLLCEVLSETAELLRNGPTARKIKIGLTTVGSEVAPL
                     DLLRGAEIAMQQVPGLEVSLIGLVPKSNLPVYEAVDEDEVHAVLERLLDDKDLDGAVT
                     MHYPFPIGVATIGKVITPARGRNMYIASTTGTSDTDRIQAMVKNAVLGIAAARADGIA
                     NPTLGILNVEGSRQVERYLNAMQDKGYDICWGTSQRADGGQILRGNDLILGSVDVVVC
                     DTLTGNILMKLFSSFTSGGDYETRGYGYGPGIGENFDRLICIISRASGVPVIAGAINY
                     CAAMVRGGLKQVASQEITKAQQAGWKIPASPVTVPAKEEINPPPAKITAEQITGIDIL
                     ELEDAVKTLWKNNIFASSGMGCTGPIVLVAGEDKANALEILKVHQYI"
     gene            complement(38361..39929)
                     /gene="grdC"
                     /locus_tag="P5549_00960"
     CDS             complement(38361..39929)
                     /gene="grdC"
                     /locus_tag="P5549_00960"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HRW11676.1"
                     /translation="MNFPVIKAVGFILVHAPNIMMEHGTTITMERKNNPQSEYLHKAS
                     QSLRSFEEAVQYAPNQVFIGNLTPHELQEMPKPWYQNLINNDRQGKYGEIIPENEFYA
                     VMKIADSFQLVELEEAFAARSKEILTAKRMFNEKQLGFLNKASSRSYIEELVNSGKAE
                     GLYENNQLVGCVRQAHESDPNLSATIVFENLVAKASGILALKNLLRKNNLDAAQIDYI
                     IETSEEAIGDMNQRGGGNLAKAIGEAAECNNATGVDMRAFCAGPAHGILTAAALVQSG
                     IFKNVVVMAGGSSAKLGMNSRDHVNKEMPVLEDMVGGYAILISENDGRNPIIRTDIVG
                     RHKISSGSSPQAVIQAIVVDPLEKVQLRLIDVDVYAPELQNAEITSPAGAGDVPLANY
                     KMIGAMAVKRGEISRDELPSFVSQHGVTGFAPTQGHIPSGVPLLGYIRDNILTKKMQR
                     AMLIGKGSLFLGRMTDLFDGISFIIEPNQGIQGDCMPSDNKEESKQEIRNMLADAMQE
                     LARSMSGGRLSNGE"
     gene            complement(40029..41333)
                     /gene="grdB"
                     /locus_tag="P5549_00965"
     CDS             complement(40029..41333)
                     /gene="grdB"
                     /locus_tag="P5549_00965"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(40287..40289),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HRW11677.1"
                     /translation="MAIRVVHYLNQFFGQIGGEEKADTIPFCKNGPVGPGTALVNALD
                     KDMTIVGTVICGDTYFNENIDIATKEIVGLVAGFNPDLLIAGPAFNAGRYGLACGAVC
                     TAVQKKLQIPVVTGMYEENPGADMYKKDIYIVKTGNSAAQMRNAIPALARLASRIANG
                     DVIGSPAEEGYLARGIRKNVFHEQRGSARAVEMLLKKLKGVPFVTEYPMPVFDRVSPR
                     AAIKHLSQAKIALVTSGGIVPKGNPDRIESSSASKFGRYDISGMNDLTAETYETAHGG
                     YDPVYANQDADRVLPVDVMRKLEQEGIIGQLHNIYYATVGNGTSVANAKKYAHAIGEE
                     LKHAQVDGIILTSTUGTCTRCGATMVKELEEVVDVPVVHVCTVVPISLTVGANRIDPA
                     IAIPYPLGNPQLNQEEEFALRRSIVEKALQALTTEISEQTVF"
     gene            complement(41351..41815)
                     /gene="grdA"
                     /locus_tag="P5549_00970"
     CDS             complement(41351..41815)
                     /gene="grdA"
                     /locus_tag="P5549_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099188729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(41678..41680),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HRW11678.1"
                     /translation="MDKSFFTDKKICILGDRDGIPGPAIAECMETVGGEILFTSTECF
                     VUTAAGAMDMANQLRIKETADKFGTDKVVVVLGGAEAESAGLTAETVINGDPTYAGPL
                     TNIALKLPVYHILEIKDLIDPAIYEDQISMMEMVLDVDEIKKEMENIRQQFC"
     gene            complement(41857..43140)
                     /locus_tag="P5549_00975"
     CDS             complement(41857..43140)
                     /locus_tag="P5549_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HRW11679.1"
                     /translation="MQLELRNILVKDVQFGDKSRIENGIVYIDRLELLNTIKDDRLAR
                     IEADIVHPGDEVRICPVKDVLEPRVKVDGPGQVFPGLFGNAEMAGSGKTNVLRGMTIL
                     TVGKIVGFQEGIIDMSGPGADYTPFSQTINLVILCDKAEGLPQHEHEEAIRMAGLKAT
                     RYVAELGKNAVPDQTQLFETKPLLEQIKQYLDLPKIVYVYMLQTQGLMHDTYLYGLDA
                     KRILPTFLYPTEVMDGAIISGNCVSACDKNTTYHHLNNPVIADLLARHGKDLNFIGIV
                     VTNENVTLLDKERSSNFTAKLVESLGADGAIISEEGFGNPDADLIMNCRKLESLGIKT
                     VLITDEYAGRDGASQSLADANPLANAVVSGGNANALIHLPSMSRVIGYIEPSQIIAGG
                     FDGSLQQDGSLTIEIQAITGATNELGLNRMGALEY"
     gene            complement(43156..43473)
                     /locus_tag="P5549_00980"
     CDS             complement(43156..43473)
                     /locus_tag="P5549_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRW11680.1"
                     /translation="MIAVNKDNFDNEVLGASGIVMVDWWGPKCERCLELMPHVEDLAP
                     KYPQIKFCSVDTSGNRRLAISQKVLGLPAILFYKDGQKIDELSGQEIAPEDIEAKLKQ
                     YVE"
     gene            complement(43488..44705)
                     /gene="trxB"
                     /locus_tag="P5549_00985"
     CDS             complement(43488..44705)
                     /gene="trxB"
                     /locus_tag="P5549_00985"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRW11681.1"
                     /translation="METIYDLLVLGGGPAGLAAAMYGARARMHTAIIEKGRPGGQAAT
                     TEELENYPGFGRGTTGPGLTQAMAEHAQSFGAELIKDSIVSLDVKSFPKKVTGKKGEY
                     LGKALVYALGAEPRMLMVPGEGKLRGKGVSYCATCDADLFEELEVAVVGSGDAAIEEA
                     EYLSKFADQVSIIVIHEEGKVDANQVSFERAQQNPKIKWVWNSIVKSVNGDELVESIT
                     LKNIQTGEESDYPVNGVFIFIGTVPLTDIFPAEVQLNPQGYIITDEKMATSLEGLFAA
                     GDVRAKYLRQVVTAASDGAIAAVAAEKYVHETELWKNQVIEAKKPVLVAYYNPADMSG
                     TEKIGIVDRFVRANQERFSLVKVDATRNELVGRRYHITPDQLPKLHVFMNGEIKQEVA
                     EINEESLNAIKNM"
     gene            complement(44708..45100)
                     /locus_tag="P5549_00990"
     CDS             complement(44708..45100)
                     /locus_tag="P5549_00990"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HRW11682.1"
                     /translation="MQSGQFLIITNNPIVKNIPNAEIQFVIGNQRQVFYAALNKAAGG
                     HRLLSHPLAGSIKPADNPYRSMVLTALATEVDYAALNMLTYGLERMEAENTNRRDNLI
                     IPQLDEDFQMIDKALLDQALPSLTERRV"
     gene            45372..46265
                     /gene="spoIID"
                     /locus_tag="P5549_00995"
     CDS             45372..46265
                     /gene="spoIID"
                     /locus_tag="P5549_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HRW11683.1"
                     /translation="MKKKLLITGLLFVFILSIWRYTVTKPEPVPEPFVKLYLHQKNEV
                     VDLQMETYIIGTVAAEMPASFGMEALKAQAVCARTYAVKKISEKHPYPRQADLSDDIN
                     CCQAFCDLAEHHLNPENLARIKTAVQETRGEIMLYDSRPIDALYHSCCGGRTDSAWGS
                     SSQLAYLKPVKCNHCQESRHFYEKHQFPNHQLAALGGDNKQSLEIKILSFTPGGRANQ
                     ISINGKKIWASELRRQFNLPSQWLSFKVGRQQTEITTRGYGHGIGLCQYGANGMAKKG
                     YDYREILNHYYQDIKIYRLPY"
     gene            46368..46634
                     /gene="spoIIID"
                     /locus_tag="P5549_01000"
     CDS             46368..46634
                     /gene="spoIIID"
                     /locus_tag="P5549_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HRW11684.1"
                     /translation="MQNYIRKRAVKLARHIVQTSDTVRATANAFGISKSTVHKDVTER
                     LPRISEELFLQVKHILDKNKSERHLRGGEATRKKYAKAAEFEVL"
     gene            46706..47731
                     /gene="mreB"
                     /locus_tag="P5549_01005"
     CDS             46706..47731
                     /gene="mreB"
                     /locus_tag="P5549_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HRW11685.1"
                     /translation="MCFVEDIGIDLGTASVLVFKKGEGIVLHEPSVVAIDKNTNKVIA
                     VGEEAREMLGRTPGYITAIRPLREGVIADYDTTEKMLSYFVKRVLGNKLFMKPRVIVC
                     IPTGATDVEERAVRQATLAAGAKQAYIIEEPLAAALGAGINIAGATGNMVVDIGGGTS
                     DIAVLALGGIVCNTSLRVGGDKFDEALIRFVRREYSLMIGERTAEEIKMRVGTAWITS
                     ENRDRYMEVRGRDLVSGLPKTINISSQESWIALEEPVYAVIDAVKKVLEITPPELAAD
                     IVDNGIVMTGGGSLLEGFSTLLSVETNLPVHVADDAISCVAIGTGKALNEIHVLSKNG
                     RGPKRLV"
     gene            47857..48597
                     /locus_tag="P5549_01010"
     CDS             47857..48597
                     /locus_tag="P5549_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRW11686.1"
                     /translation="MEVRKYADILGARVDLVNTRQAMERIKSFIAKRQSAHVITLNAE
                     IVYQAQDNPELLKIINGADLVTPDGIGIVWAGRHLGYAFEERVSGIDLLELLCEQAPQ
                     KNWKIYLLGAAPGVADEAARQLTLKYPGIDICGTQHGYFTAAHQQSIVEVIQQLQPDI
                     LFVGLGAPGQEIWIHQFQLALAVPVAIGVGGSFDVVAGIKSRAPQWVIKANLEWLYRL
                     LKEPRRLKRQLALPRFVWAVLRQKKIKN"
     gene            48620..50758
                     /locus_tag="P5549_01015"
     CDS             48620..50758
                     /locus_tag="P5549_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HRW11687.1"
                     /translation="MDNDKIFALDIGTRKILGLVMQKGADGYEVLDVEMIEHQTRAMM
                     DGQIHDVETVANTILTIKKRLEKRLQIQLSSTAVAAAGRSLQTTHGYAVGNHPQPAEL
                     SRDEVRTLEIEAVQNAQYAAARDEKTHLSHAEYFCVGYSVIHYFLEDEPIGNLIGQKG
                     SSYAVEVIATFLPRVVVDSLLSSLQRAGLEVYSITLEPIAAISLAIPANMRLLNLALV
                     DIGAGTSDIAIVKHGSIYAYAMVPLGGDELSEELASSCLLDFNSADWLKCQIATQDEL
                     HIKDILNNDLCIKAEDLKKELEPVTRELAREIASHILQLNGKAVDAVLCVGGGSLTPN
                     IGQFLAEALELPRNRVGIRSRDNVSQIKGDFSCLQGPQSLTPLGIAFSALEKPPVPML
                     KVSVNGRELAIWNAGEITVADALLSSGISLGNAYGKPGLGKTLEVNGSLKVFKGEIGT
                     ASSIRLNGKEANFDIPIINGDQIEFIRGKDGRDAKVTLQDLDTGGKGSVYINGEKMEL
                     QPVMTVNGQLWRTDAELPDRSKVEIHRTSRLEQIMRQFGITETWLQEQPYHFYFNGNL
                     RQGKWTALQIKVNGQPAQLDETVSFGSYIEYRMDQKRPCIADLVELPDNHDFTVYVNG
                     EKLTLQGKGYRLIKNGAVVAAQTEVVRGDQIELLPEESQLILSDIFQSINIEKQPQGI
                     LHIKVNGEEAGYTTPLADNCEVTLTWERAD"
     gene            complement(50755..52044)
                     /locus_tag="P5549_01020"
     CDS             complement(50755..52044)
                     /locus_tag="P5549_01020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRW11688.1"
                     /translation="MSLSCKSLFSILLIGLYLFLPVSHFSAAGTVDVSSLSAASYVLM
                     DAESGKVLKSHLPHQQLPPASTTKLMTMLLALESIEQGQIAKTELVTASRKASETDGS
                     RIYLEVGETMRLDDLLKSMALASANDASVAVAEKISGSEAKFVTRMNSKAKEIGMQDS
                     HFVNVSGLPAEHHYSSAYDLALLARYTLAHTEILNYSSLKQHTLRDGTFPLYNGNRLL
                     WTYAGADGLKNGYTSEAKNCLIATAQRGHLRLIAVVLGCPLRGGQTSDVTHLLDYGFD
                     KYDAIMLIPRGEICGTVRVKAGSVKEVEAVLQDELNAIYAKSDGIHFTRRLELPEKVE
                     APVTKGQVLGKMEILSNSKQLMNVELIAAKDIPRLSLLGQFLHMHWILKLLLFFFLLT
                     AFLFYARHRIHKNGGRWKKPKSRIRHPYDQHYQDYRY"
     gene            52285..52710
                     /locus_tag="P5549_01025"
     CDS             52285..52710
                     /locus_tag="P5549_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRW11689.1"
                     /translation="MKKSLADRIEQYLKVLISRSDDKAIEIQRIELAETFDCVPSQVT
                     YVISTRFTEKEGYITESRRGGCGYVRIRQSPPSFGEDDLEYIDLFAFLAELKQRQLLS
                     NREAELIKYLVVNTCASLPGEDKRKINAAMVKALYNYFV"
     gene            52766..53272
                     /locus_tag="P5549_01030"
     CDS             52766..53272
                     /locus_tag="P5549_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRW11690.1"
                     /translation="MYCEECKQKPAAVHLTQVYNGKIIQSHLCEECAAKKGGFIFDPG
                     NKFSIPNLLSSIFGASCSIDPDLTTEIKSCSGCGMTFNDIRQTGKLGCSNCYQVFAQE
                     MEPTLRRIHGNSQHIGKIPTRSGEKVLLRKQLDALKTQLQEAVNHEEYEKAAEIRDGI
                     KALESRLV"
     gene            53286..54353
                     /locus_tag="P5549_01035"
     CDS             53286..54353
                     /locus_tag="P5549_01035"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRW11691.1"
                     /translation="MSIENILNCGSVKWMESQEGQDDIVISSRIRLARNVVDLPFPMR
                     LDQKSGDECMQRVKAVCQEEMPAALNTVEYTSLAQLSILDRGILAEKHLISPGHAESD
                     SPFGAILLNRDGSLATMVNEEDHLRIQCLLPGLQLAAGYEMAQQMDDFLESKLEYAFD
                     ERNGYLTSCPTNVGTGLRASVMLHLPAMQMTGQTGQILQTIGQLGMTVRGLYGEGTQA
                     TGNYFQMSNQITMGQSEEEICNHLQAVTEQIVEQEHLLRERLKIDLKYQLEDKIGRAL
                     GILNHARIINSTEALALLSDLRLGIDLGIVSGIQLNALNQLIVSIRPAHLQKCFAQEL
                     DPLQRDIKRAEIIQDKIKGIK"
     gene            54383..56860
                     /locus_tag="P5549_01040"
     CDS             54383..56860
                     /locus_tag="P5549_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRW11692.1"
                     /translation="MFRRFTQRARNTVIHAQEEARELGHQAIGTEHILLGLLREGEGV
                     GAKALMNLGVDLENVREEIKKYIGQNETDNQEAANDLPITPRVKKVFNNAFDEARMQG
                     VNYVGTEHLLLAILREEESVAGQVLLSMGVRLDDLREQVILLLGGEFPGMMNQEPNQE
                     PSVPSSGQGQKIRKTKSKTPTLDGYSRDLTKDAQDGRLDPVIGREDEIERVLQILSRR
                     TKNNPVLIGDPGVGKTAIVEGLAQRIFENKVPENLSHKRVVSLDLSSMIAGTKYRGEF
                     EDRLTKIVSEIKTAGDVIIFIDELHTIVGAGAAEGAIDAANILKPSLARGEFQCIGAT
                     TLNEYRKHIEKDAALERRFQPILVDEPSIEESIEILKGIRDRYEAHHGVKISDEAIAT
                     AVRLSSRYISDRFLPDKAIDLIDEAASRVRLASFILPPEIKELEARMDELTKEKEEAI
                     NAQEYEKAAQLRDEEQRLRDQVDNMRKDWVGKRSTTAGAVGEEEITNIISRWTGIPVS
                     KMAMEESERLLQLEEILHGRVIGQDEAVKAVARAVRRARAGLKNPKRPIGSFIFLGPT
                     GVGKTELARALAEAMFTSEDAVIRLDMSEYMEKHAVSRMIGSPPGYVGYDEGGQLTEK
                     VRRKPYSVILLDEIEKAHPDVFNILLQVLEDGRMTDGQGRTVDFRNTIIIMTSNVGVE
                     NLQRNKAMGFTRVVDEAEDYNRMKENVMEQLKHTFRPEFVNRIDELIVFQSLTEDELK
                     QIVALLLKDLRQRLQDSGYSLEISEEASKLILKEGYDPAYGARPLKRAIQKLVEDRVS
                     EEILKKSIKPGDTIKLNAVQGEMTVNG"
     gene            56959..58314
                     /gene="radA"
                     /locus_tag="P5549_01045"
     CDS             56959..58314
                     /gene="radA"
                     /locus_tag="P5549_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRW11693.1"
                     /translation="MAKALSKFVCRDCGFETAKWLGKCPGCGAWNSLLEERTAPAGKS
                     RAIRTIEALALNSIPQTQVYRFSSGMNEFDRVLGGGMVPGSLVLLGGDPGIGKSTLLL
                     QVADNLAQLGRKVLYLSGEESPLQIRLRSVRLGIETDRIYLLNEQNLDLLDSYLTEID
                     PDLVIIDSIQTVYCESLSSIPGSVSQLRECTAKLMTMAKHSQRVFILVGHVTKDGALA
                     GPKVLEHMVDVVVYFEGEKNYSFRILRGVKNRFGPTDEIGLLEMSGQGLMEVLDPAQI
                     FLNTNEPPAGGSAVVASYEGSRPLLIEIQALVALSGPGYARRMASGVDQNRLALIIAV
                     LEKKWGLNLSAYDVYLKVTGGVFIKDPSVDLGIAAAILSSFKEIPLPRDMVFLGEIGL
                     SGDIRSVPFLDNRLKEANKMGFHQVVLPFGGVKSGWEETGLHIQGIRNLDQLLEIIME
                     G"
     gene            58317..59390
                     /gene="disA"
                     /locus_tag="P5549_01050"
     CDS             58317..59390
                     /gene="disA"
                     /locus_tag="P5549_01050"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HRW11694.1"
                     /translation="MAEDINRTNFRKSLKLLAPGTIFRVGIENVLQANTGGLIVVGDS
                     PELMSLVSGGFKIDCEFTPSRLYELAKMDGAIITNHDASRIIQANAQLDPNPNLPTRE
                     TGIRHRTAERIAQQTGMLVVAISQRRSVVTLYQGNTVFRLRDMASILVKANQALQTLE
                     KYRNVLIRELSHLGGMEFEDVVTVAEVCGVLHRCIRVLNIAEEIENYIAELGSEGRLV
                     KMQLDELVVNVREEAILIIKDYSHTPDKSPQELLNTILRAFEEEISDSVFIARILSLG
                     TTSSHLEQMISPRGYRMLNKIPRIPLSIIDNLVEKFNMLKYILPATIEELDDVEGIGE
                     VRARSIKNGLLRMQEQIMPEYLV"
     gene            complement(59472..59870)
                     /locus_tag="P5549_01055"
     CDS             complement(59472..59870)
                     /locus_tag="P5549_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRW11695.1"
                     /translation="MKDLICDEFQNTVDNLLIRHHSVLDVTSKLNEATCRVNRSVIKA
                     VTDCGCVTVEAKKIQLPDNIESISELKSYLDNHLRGQLCQQCREVVVSELGKLLFYTA
                     ALCNTLDINLYDVFINEYKEAEALGIYNLR"
     gene            60118..61230
                     /locus_tag="P5549_01060"
     CDS             60118..61230
                     /locus_tag="P5549_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRW11696.1"
                     /translation="MYKRLLLGVLLLFSLVLGMWISSLADQFLHLPVLWQATVGVAAT
                     GMLYVLLQLILHMAGHGISKFNHVLDNTSLEMLLGGLLGLTAGLLLGLLTGYPLALLS
                     ELGGYLTLGIFMLCGYWGLRTGIHRAQEIWQLLPLSRISHKPLSDSCGSCKVIDTSAI
                     IDGRIYDVALSKFLEGNLLVPLFVVEELQHIADSSDSLRRGKGRRGLELLSKMNQHAD
                     IKIDIIETDIPEEKEVDMKLIKLCKQMNASIITNDYNLNKVAELQGITVLNINELTNA
                     VKVVVYPGETMSINVLKAGREPGQGVGYLEDGTMVVVEDAQHELGNSLEVLVTSVFQT
                     AAGRMIFTRKIKEEETAMESNLVLPNVKSQEVNLYG"
     gene            61223..61918
                     /gene="ispD"
                     /locus_tag="P5549_01065"
     CDS             61223..61918
                     /gene="ispD"
                     /locus_tag="P5549_01065"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRW11697.1"
                     /translation="MADNLRVVIAAAGQGSRMGSKTNKQFIMLNHKPVIAYSLDFFER
                     HNAVDEIVVVTSARELEYCRDEIIARNKYQKVSAVLAGGQERQDSVWAALQYLARDTD
                     YVAVHDGARPLLSTDVLNRLLEAAEDWGAAIPGVPSRDTVKMINKDSFVRQTLDRSVV
                     YSIQTPQVFKFKELYTAYKYAEEEDFYATDDAALFEKYIGQVKVVEGSYDNIKITTPL
                     DLVFVQTLLNRMD"
     gene            61937..62416
                     /gene="ispF"
                     /locus_tag="P5549_01070"
     CDS             61937..62416
                     /gene="ispF"
                     /locus_tag="P5549_01070"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRW11698.1"
                     /translation="MRIGSGYDVHRLVEGRRLILGGVEIPFSRGLLGHSDADVLLHAI
                     CDALLGAAALGDIGQHFPDTSEEFKDIDSRVLLCRVGNLLRDRGWRIENIDTILIAQA
                     PRLAAYLPAMCRNIAKTLNMESDQVNVKATTTEGLGWEGQGEGIAAQAVVLIDKGKD"
     gene            62429..63877
                     /gene="gltX"
                     /locus_tag="P5549_01075"
     CDS             62429..63877
                     /gene="gltX"
                     /locus_tag="P5549_01075"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRW11699.1"
                     /translation="MKKIKARFAPSPTGPLHIGGARSALFNYLFAEHYGGEMVLRIED
                     TDLDRSHREYEEEIITSLQWLGLTWQEGVNVGGENGPYRQTERLDLYRQYVQQLIEQG
                     SAYYCFCSQEELEAERQEQYAQGLTPRYGGKCSQLDKEEVNRRLLAGEPASIRFRVPQ
                     NTIYVVNDLVRGTVSFESGNSGDFIIVKSDGIPVYNFAVVIDDHLMGVSHVIRAEEHL
                     SNTPRQLMLYDALKFPRPEFAHISLILGDDHQKMSKRHGATSLVQYRERGYLPEALFN
                     FLALLGWSPEGEKEILSKEEIVKGFTLDRVSKSPAVFDINKLNWMNQQYIKQLSTPAL
                     IDGLLSYIQNSRYADDWARLSADRTVVLVEAIREHLVCLGDVEQQMEVFFQPLAYEAE
                     AWEVLQQAHVMQVLKSVHNTLPEFSKPQEIKQFIKTIVKTTQLKPKDVFMPLRSALSG
                     RTHGPDLPNLIYVWGREQALARLRETMRKLGG"
     misc_binding    63924..64145
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            64193..65800
                     /gene="cysS"
                     /locus_tag="P5549_01080"
     CDS             64193..65800
                     /gene="cysS"
                     /locus_tag="P5549_01080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRW11700.1"
                     /translation="MKVYNTLSGKKEELRTIAANQVNIYVCGPTTYNFIHLGNARPLV
                     VFDTVRRYLAYRGYTVKYVQNFTDVDDKIINRARKENDDPLQLAKRYIDEYFVDADAL
                     GICRADVHPRVSDHIPEIIGVIEGLMAKGYAYEVNGDVYYRVKNFSGYGKLSGRSLDE
                     MLAGARVEVDERKEEAADFALWKKTKEGEPSWPSPWGEGRPGWHIECSVMSMKYLGET
                     IDIHGGGADLIFPHHENEIAQTEAYTGKPFVKYWMHNGFITVNSEKMSKSLGNFFILR
                     DILEKFPHDVVRYYLVATHYRSPLDFDDGKLEEARKALSRLKTTLNLAEEFLLHHDLD
                     MEKQTLPDTEGELSGSVKALQEKYITAMDDDFNTAQAIGHLFEISHLLNSFVANADPK
                     SVREQVQLQQALQIFRDLGGILGIFMETKNEDLAQAVDAVLDILLEVRNYARQEKNYG
                     LADSVRKSLQTMGVKVEDKAIGESVCRYESAPEMELLMGHILQMRRDLKKQKNFQQAD
                     YLRDRLKTAGIIIEDTKEGARWKFADA"
     gene            65787..66182
                     /locus_tag="P5549_01085"
     CDS             65787..66182
                     /locus_tag="P5549_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRW11701.1"
                     /translation="MLTHNLVDEKTLRQYSPLVLAYVGDAVFELAVRTRIAGNGPRRL
                     RDLHHEAVATVNADRQAAIVRQMEAQLNQEELDIVRRGRNTRSHPPKNADVQSYRMST
                     GFEALLGYLYLKGEHDRLDYLLHKALEEI"
     gene            66262..66954
                     /gene="thyX"
                     /locus_tag="P5549_01090"
     CDS             66262..66954
                     /gene="thyX"
                     /locus_tag="P5549_01090"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRW11702.1"
                     /translation="MIILQPQVLIPESELDANLFRKLERYARTCYKSEDKMKDAGDPH
                     FLKSKLTMGHESIIEHEKATVMFIVDRGISHEIVRHRIAAYSQESTRYCRYSQNKFGN
                     EISVIEPYFFKNRSDDYQLWKEACLGAERNYMAMLESGCSAQEARSILPNSLKTELVV
                     TYNLREWRHFFRLRADIAAHPQMRQAAIPLLLYFQEKMPALFDDVNYDRDFPVEHYAQ
                     IIQTDEVFNPLA"
     gene            66997..67710
                     /locus_tag="P5549_01095"
     CDS             66997..67710
                     /locus_tag="P5549_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRW11703.1"
                     /translation="MSAHRRWLILLLAAAGLVLIFQYKESLIARFDYHTLLEQVALEP
                     ECKVPRLISSRDNNYNGIPDPLDIVAGARQEVEQGTLYDSSYYQGGFPPEGRGACTDV
                     VWRGFQAAGLDLKQMVDSDIRNQPQLYGATGTNPDPNIDFRRVSNLQIFFQQYAQQLT
                     TVMIPGNAENLSNWQAGDIVIFGAPLEHIGIISDKRSRNGVPLLIHNAGPRASEGQYI
                     LNWPSPLTHHYRYIPYDEQ"
     gene            67732..68472
                     /gene="rlmB"
                     /locus_tag="P5549_01100"
     CDS             67732..68472
                     /gene="rlmB"
                     /locus_tag="P5549_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRW11704.1"
                     /translation="MQEKLAGVNSIMEALNGRRKIHKIFVQEGRGGKRIEELLKLAEK
                     RGVYWQYVDRQRLDQMYTQANHQGIIAQVDSYTYASVEEILENAALSGQEPFVLILDG
                     IEDPQNLGSIIRTAECAGVHGIIVPRHHSSEITPAVVRSSAGAVEHILLAMETNLVNT
                     IKSLKKRGFWIIAADMDGEQDYFCCQMPTPVALVVGGEGQGIRRLVKENCDLVLKIPM
                     VGAIQSLNASVAAGLIIYEVLRQRYNQQ"
     gene            68603..69259
                     /gene="sigH"
                     /locus_tag="P5549_01105"
     CDS             68603..69259
                     /gene="sigH"
                     /locus_tag="P5549_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HRW11705.1"
                     /translation="MIGSVAARQLYVDYVEMSDEEIVELAQLGNQFAIEFLVDKYKNF
                     VRAKARTYFLIGADREDIIQEGMIGLFKAIRDYNGEKLTSFRAFAELCITRQIITAIK
                     TATRQKHIPLNSYVSLNKPVYDEESDRTLIDIITTNKITNPEEIIISREEFIYIEKKM
                     GEILSSLEWKVLMAYLEGKSYQEIAQELKRHVKSIDNALQRVKRKLEKYLEDRNEINR
                     "
     gene            69382..69457
                     /locus_tag="P5549_01110"
     tRNA            69382..69457
                     /locus_tag="P5549_01110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69415..69417,aa:Thr,seq:tgt)
     gene            69469..69553
                     /locus_tag="P5549_01115"
     tRNA            69469..69553
                     /locus_tag="P5549_01115"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69503..69505,aa:Tyr,seq:gta)
     gene            69564..69639
                     /locus_tag="P5549_01120"
     tRNA            69564..69639
                     /locus_tag="P5549_01120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69597..69599,aa:Met,seq:cat)
     gene            69770..69845
                     /locus_tag="P5549_01125"
     tRNA            69770..69845
                     /locus_tag="P5549_01125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69803..69805,aa:Thr,seq:ggt)
     gene            69849..69925
                     /locus_tag="P5549_01130"
     tRNA            69849..69925
                     /locus_tag="P5549_01130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69883..69885,aa:Met,seq:cat)
BASE COUNT        19222 a        15220 c        17485 g        18063 t
ORIGIN      
        1 cttgcctgaa aactagaagg ggatccggtt ggtgcgtgtc caaagcttca tttctttggc
       61 tgatttaatc agatcatcgc ggaagtcggg atgggcaatg ctgaccagag cgtctgcacg
      121 accccaaatc gacagggctt tcagatttac ctttccgtat tcagtaacca accattgtac
      181 ctgggtgcgg ggtacggtta caacgccacc gggggtcagc aaaggtttaa tccgggaatg
      241 cattttacct tccttgtcct tgtaggtgga agagaacgcc aggaaggcct tgcccccttt
      301 ggagtgaaat gcaccgacca cgaagtccaa ctgtccaccg gtaccggata tctgccttag
      361 acccgaggtt tccgaacaga cctgggatag cagatcaatt tccaggatat tgttgatgga
      421 aatgtaatta tcgttcatgg cgatcaagga aggctcattg gtataatata cgttacagga
      481 agcacagcgg ggattgtcgt tcaagaagtc atacgtctcc tgcgtgccca aacaaaaggc
      541 ataagtactt ttatataaat ccctggcttt tttggcattg gttatttttc cggcttcata
      601 catggcaaca taggcatcac aaaacatttc actctgcaca cccaaatgtt tgagatctga
      661 atcggctatc atatggccga tcagattggg cagtgccccg atgcccagct gaataactgc
      721 cccatcaact acttcttcaa cgacatattc tgcaattttt ctttcttcgg gagtgggctg
      781 ggcggatggg ggagtggaaa atatgggtgt gttggaacct tcaattatat agtcaatttc
      841 gctgatatgt acatactcat cacaaccgcc cagacagcgg ggcatatttt cattgatttc
      901 cactattgcg attttggagg ccaaggccag gccccggtta tgggaattgc ccagaccaaa
      961 gttaaaacat ccggaactgt ccatcggtgt tacctgggca caccagacgg aaggccaaat
     1021 atgggaaaaa tctgtgtttg cggccaacat ggtagcttcg tgatagttga agggattgtg
     1081 gcagcaaagg ccgtaatctc cggcagtacg gtcaatcaaa gtataatacc aggaaaaata
     1141 ctgaaaggtt tgctgctgcg gatcgttttt aattacttcc ggaaccggca gaactgttcc
     1201 ggtacctctc aaggaaacgt tttcaaggcc atctccgacc cttttgctga gtgctacatc
     1261 aaaatcaacc ggtttggtcg caaacattcc ataatcaatg atagagttgg atgctaccag
     1321 cgaggcggct ttttcagcac taatcagttt acttctgtac tcatccatat aagtacccaa
     1381 gtgtctcccc ccttattttt tgaacctgca agaattcaag tgcaatcaat caactgaaaa
     1441 ttcaaaaaaa tattacatta tctattattc ggacaaaaat acaaatttcc ttcctgaaag
     1501 aaaaaaatta tatttgatac aattatttgt attcaataaa ggaaaagata acggtatgat
     1561 cctgcgcgaa taatagcagc aaaccaaccc cgcatgcttc gtaaaaaacc aacaaaatgt
     1621 gaacattgcc cgggatctga tatataatta actcataaat aacaggttta cccagaaaaa
     1681 taaagggagc aatctatgta cacgactaga attattacta tcgacgaaaa ggaacgctat
     1741 aacgattttg tccgccagca tcccaagggc cattttctgc aaagctggga atggggtcaa
     1801 gtcaaacggg gcatgggatg gatgccgttg ccgcttatcc tggaaaaaga cggagagata
     1861 gaagcgaccc tgctgatttt gaaacgagcc ttgcccttgc cggggttaaa taaatccatt
     1921 ttctattctc cgcgcggacc ggtaatggca ttggatgatc cagctctggc ttgggaatta
     1981 ttcgctggtg tgcgtgcagt tgcccgtcag catggggcga ttttcctgaa aattgacccc
     2041 gatgtgccgg tgagtgacca ggtatttgcc gatatcctga aagaatgcgg ctttgccagg
     2101 aatgaaacgg gtttggattt cgaaggggtg caacccgctt ttgtttttcg cctcgatata
     2161 actccttcag aagaacagct attaagcaat atgcacgcca agacccggta taatatacgg
     2221 ctggccgata aaaaaggcgt acaaataagg aaagccaaag gcaaaaagga tctgcgtgtt
     2281 ttttatgatg ttttgctgga aaccgctgaa cgggatcgtt ttctgatccg tggctatgaa
     2341 tattttgcaa tgatttggga acacatggtt gagaatgact tgggcgaaat atttctcgct
     2401 gaatgggaag gcaaggtcgt tgctgccacc ctggtcatgt gcatggggga caaagcctgg
     2461 tatctgtacg gggcgtccag caaccagtat cgcaacgtta tgcccaatta cctgatccaa
     2521 tggtccatga ttcgctgggc ccgggaaaaa ggctgcacgt tatatgattt tcggggcgta
     2581 tcgggcgata tggatgagag caatcccctc tacggtttgt ataaatttaa aaaaggcttt
     2641 aacggacagt ttaccgagtt tgtaggcgat tgggataccg tttatgacaa gtttttctac
     2701 ttcttgtgga ccaaggccct gccagtttat cgcaaaagca cgcgactgtt gctggctaaa
     2761 agaaaagaag cggaggcaga ttagtgcagc cgaatcttgc ggataaatgg atcgaaatca
     2821 acctggatgc aattgaaaac aatcttcatg ccatcaaaac gagattggcg gagggtgtcc
     2881 gtctcattgc ggtgattaag gcagatgctt atggccatgg agccatcgca acagcccaac
     2941 tgctgcggga actgggggtc acctgctttg ccgtcagttt ttttcaggaa gccatgcagc
     3001 tcaggcaagc gggagtaacg gatagcattt tggttttcag ccctatcgta tccacagagg
     3061 aactaaagga tgccatacaa aacaacttga ccataaccgt tgcttcgcca tacgactggc
     3121 agttacttaa tcaagcgagc gctttgttgg atgagagggt gagggttcat cttaaagtcg
     3181 ataccggatt ggggcgtttt ggtttgcggg aaaaagaaat catggaattg ttaccggaaa
     3241 tcaagcgcaa ccagcggata aatattgaag gaatttatac ccatatggcg gcagcggcgg
     3301 ccagtgtttc ctatacccgt cgccagtttg atgacttcct gcacctcatt gacaagctgg
     3361 agcaggccgg actgagagtg cccttgcgtc attgcgccaa cagtgcagtt ttgctgaaat
     3421 atccccagat gcaaatggat gcagttcggg tgggcacctt gctttccgga cagataccag
     3481 tgggggttgc agcgaccggg ttacaattgg aggatcccta tgcttttaaa acccggatca
     3541 tctccctgcg acaacacaat aaaggggatt atctggctta ttatcgcagc tatcgactta
     3601 aaaaggcggc aaaaatcgcc gtcattccgg ttggcttcag ggatggcctg gccttgcagg
     3661 ttggcaacaa accctccggc tggctggacc tgatcaagac tgcggtcaag cttatcttaa
     3721 actatttgaa tattacccgc tttggacagc aggtcagcat aaaagggaaa aactatccgg
     3781 tgcggggcaa agtgttcatg cagatggcct tggtggaaat ccctctggat caagaggttg
     3841 gaatcggtga tgaagtggtg gtgccggtga gaaagacgct tgctgatcca gctgttgcca
     3901 gaatctattg gcgagaagaa aaagtggtta aatttaccac caaagaaggt ggatcagagg
     3961 cactcgtcga attgctatag actatcgttt ttattggatg cagcttattt caatatagga
     4021 tttacgaaaa caatcggtat caaaaaagca aataactata ttggagtggg gcacatgaaa
     4081 ggaaaaatat taattgttga tgatcaaaaa ggggtacgaa gactgctgga agagcttttt
     4141 aaaaaagaag gctgggacgt taaagtggct tctgacggtc aagaggcaat cagtctggtc
     4201 aaagaaaaat tgccggatat tatcctgatg gatgtcaaga tgcccaatat gaatggcctg
     4261 gaggcatccc aggttattct tcaggcgcat agccagattc ctatcatcat gatgactgcc
     4321 tatggtgaaa tcgaggtggt aatgaaagcc ttggaagccg gcgtgagaaa atgtattacc
     4381 aaaccttttg atataatggt tttgcgcgat atggtaaatc atctgatgca tgagcggacg
     4441 gcttgaaaga agtttacatg atatgtgcag agataccttc acatgaccga gcatgtaccc
     4501 ccgcaagtta aatcgaacct cgcatgagac cgtttcttca ggcacaggac agggtattcg
     4561 ttaaaaagga ggaatgcttt ttgcgtattt ttattgattc tgctaatata gaagaagtca
     4621 gagatattga aaccatgggt attttggctg gtgttaccac caatccgagt ctggttgcca
     4681 aggaaaaaag agattaccgg gacgttatcc gggaaatttg tgcgatcgtt gacggaccca
     4741 tcagcgcaga ggtgattgcc caggattatg ctggtatgat gcaggaagca caaatgctgg
     4801 cagcgatcca tcccaatgtc gttatcaaga tcgccatgac cgaggatggt ctgagggcga
     4861 tcagggacct ccggcgaaaa ggaatcagca ccaatgccac cctgattttt tcagccacgc
     4921 aagctttgct ggcggcaagg gctggtgcga gttatgtcag cccatttctg ggccgtgttg
     4981 atgatacagc aaacgatgga ttgattctgc tggaagatat catgattatt ttcgatcaat
     5041 ttgatttgga tacagaagta attgccgcca gtatccgtca tcccgttcat gtcctgcaag
     5101 ctgcccggac tggatcccat atcgctactg taccctacaa aatcatcaaa caaatgatta
     5161 aacacccgct cacagaggcc gggatacaaa gattcctgca ggattggcag gaagcgtttc
     5221 aatgatattc caagcagaca gtgctttctc ggcggaaagc acttgatgaa gctttaatat
     5281 tggagggttt gttgtggaaa gagaactggc gctggaattt gcgcgggtta ctgaagctgc
     5341 agcacttgca tccgcccgtt gggttggact gggtgacaag gaagcagcag atgatgctgc
     5401 ggttacggca atgcgggtta tgtttgacac cgtttcaatt gacggtgtgg ttgtaatcgg
     5461 agaaggggaa atggatgagg cccccatgct ttatattggt gaaagagtag gcttgagggt
     5521 agccccgcag gttgatattg cggttgatcc gttggaaggg accaatttgg tggccaaagg
     5581 attgaacggg gcgatcgcgg tgcttgcttc tgcaccaatg ggtacactgt tgcacgcccc
     5641 ggatatgtac atggacaaga ttgctgtcgg agcagattgc aaaggacggg tttttttgga
     5701 tgctcccgtc aaagagaacc tgcgtcaggt tgccaaagca ctggacaagt cgatccgcga
     5761 agtgacagta gtcatactgg accgggaacg tcatcaggga ataattgatg aggtcagaag
     5821 tgtgggtgcc cgcatccgct tgatcagtga cggcgatgtt tcccccgggg tggcggccgc
     5881 ctacgggaat tccggcgtgg atattctgat ggggatcggt ggggcgccgg aaggagtcat
     5941 cacggcggcg gcaatgaaat gtatgggagg cgatttccaa gcccgtcttt ttccggaaga
     6001 cgagaatgag atcaggcgtt gtcatgagat gggcatcaag gatgtcaatc agctttttac
     6061 gattgacgaa ctggtcaaat ctgacgatgt tatctttgtt gccacggcga taacggattc
     6121 ctatttactt aaaggtgtac aatacgccaa aggacgtgct attacccaaa cactggtaat
     6181 gcgcagcgct tccggtacga tacgttttat tgaggccaat catagtttgg acaaaaaacc
     6241 attattcaaa gacaagcgca tatcgctaac attggaatga tatactgagg taagcgattt
     6301 ttttaagtat tttactgcgt aagaattgcc tttctgttta ctggatcaac aaagggaaag
     6361 aatatataca aaggctgtac cggcctttgt atcctcaccc gggagtttgc cccgggaaga
     6421 tcttcaccca tgttacccat ttgtattgtt tgacagatca ataaccggct gacagggata
     6481 gtctgtagtg gatcaatgcc tggcagtctc ctggattttg catgcatggc atcaaatcat
     6541 atgaatacgc tttccacagc gagaggagga acattttgaa actaaacata tcggaactgg
     6601 aaaacaaaac catgctggag ctgtatcaga ttgccaagga aatcaatctg gccggttatt
     6661 caaagctgcg taaaaaggaa ctggtatttg agattaccaa agcccttacc cattccaatg
     6721 aattgctgca agcctcaggg gtattggaaa tcatgccgga cggatatgga tttttgcggc
     6781 cttttgccta tgtacccagt catgaagaca tctatgtgtc accttcccag atcaggaaat
     6841 ttgacttgcg aaccggtgac cgggtaggtg gacaagtcag acctcccaag gacagtgaac
     6901 gtttctttgc cttgttgaaa gtggaggacg tcaactccct gcctccggaa gaatcaggca
     6961 aacgcattca ttttgatgca ttgacacccc tttatcccac ccaacgtttg acgttggcta
     7021 ccgattcagc cgaactctcc acccgcatca ttgatttggt ggcgcccatc ggcaaggggc
     7081 agcgcggctt gatcgtagca ccccccaagg ccggcaagac catgctcctg caaaaaatgg
     7141 cccagggcat caccaccaat cacccggaag tggaattaat cattttgctg gttgatgaaa
     7201 gaccggaaga ggttactgac atgcagcgtt ccacgcaggc tgaagtggtt gcctccacct
     7261 ttgacgaacc cccggaaaac catgtaaaaa ttgccgacat ggtgctggag agggccaaac
     7321 gattagtgga gcacaagcgg gatgtggtca ttctgctgga cagtattacc cgtttggcca
     7381 gagcccacaa cctggttatc ccgcccagtg gcaggacgtt gtcggggggt atcgatcccg
     7441 cttccctgca caaacccaaa agatttttcg gggccgcccg caacatcgaa gaaggcggca
     7501 gtttgaccat cattgccacc gctctggtgg aaaccggatc acgcatggat gatgttattt
     7561 tcgaagaatt caagggcacc ggcaatatgg aattggtctt ggaccgcaaa ttatccgaac
     7621 gcagaatatt tccggccatc gacctgaaga ggtccggaac ccgcaaagag gaattgctgt
     7681 ttaatgccga tgagatggaa ttgatgtgga atttgcgcaa tacgttcagt gaatacaatt
     7741 ctgtagaaac gatgcagatg atgatcgata ccatgaaaaa gaccaaatcc aatgacgacc
     7801 ttctccgggc cgctccgatt atttttggaa aaagagaatc accccgcagg aatggcagca
     7861 agtattatga ataagaaact tttattttgc caagaataga aatacgctcg tatttttaat
     7921 tagggcagaa tagaggtgaa tatagtttgc atgaaaggtg gataagcctg ttactgacag
     7981 gcttgttttt gactgtttgc atccaccctg ctaatgcgcg tttaatcgaa ggcaagagca
     8041 atttatccaa aacgaatggc gagaaaacca agacggctgt tttatatcac atcgaagctg
     8101 gtgacagtct ctggtccatt gccaggcgta atggcgtaga cctgggcaac cttatgacag
     8161 tgaacggatt gcatgaaaag agtgttctgg ccattggcca ggtgattaaa attccaaccg
     8221 gcagcagcaa gttccatacg gttcaggccg gggataccat gtggagcatt gccgctcatt
     8281 atggactgcc ggtggcggcc cttgaacagg ctaacccgga taaaaaaccc caatgtttga
     8341 atattgggga tcgcctgacc gtaccccagc gcagttcagg acaattgctg caggcgtcag
     8401 aaacttcccg gggcatttct tttcccggcc tttttgcatg gccgcttgcg ggtgttatta
     8461 cttcgcattt tggctggcgg aattcagggt accatcatgg catcgatatc gccaaccggt
     8521 tgggtacacc gatcaaagct gccgccgatg gcgtcgttac atttgccggc tggaaaagtg
     8581 tttatggacg caccgtgatc atcaaacata ataacggaca aagtacgctt tatgcccata
     8641 cccagaagat tctggtgaag gagcagcaaa gggtcaaaaa aggccaggtg atcgcacggg
     8701 ttggggtcag tggcaaaacg accggccccc atttacattt cgaaataagg caaggggata
     8761 aatgtatcaa tcccttgcga tatttacaac agtaacgctt gcccccgcaa gggggctttt
     8821 ttttgtgttc agaaggggta taattgcata tatgtcgatg catcacactt tagctggtga
     8881 aaaatagaat aatcacacca agcacataca gctaatttgt gatctgttta ttaaaatata
     8941 gaatttgagg ttaaatcatg ttcttaacgc tttatgccaa ccaaaaaggg gaggccatgg
     9001 aaaatactga agtaatcatg ctggggcgca gtggctcgga gtgggttgaa gtggcaccag
     9061 atgaaatgat ccctttgccc aagggagctt cgctggtatt attgcctggg tttgttccga
     9121 ttggattgga ccggcaaaca ggagatctgc aatattttac ctgcctgccc gatgataaac
     9181 gcgaaccggc aacagctgtg gcagcactgt tgccgcaggg ctttacccgc acattgctgc
     9241 cggcctgtgt cagtcccgaa gcacaaccgt tgcccttgct tggttatgcg gcagtgggat
     9301 tcaaggacaa ccaaatttgg gtagcggcgg ttaaaagcga cgaacatcgc aaatggcatc
     9361 cccattacta taataccgct gctctgccgc agcgaatcga ccgtatgctg cgcaagtatc
     9421 ctgacaatcg aattctgcgc caactggcgc gttgttccct gcaatacagc tgctataccg
     9481 cccaaaatct tttttatggc cgctgggagg cgggaattcc taccatggcg gcttgcaatg
     9541 ctgactgtat cggttgtatc tcggagagcc atctggaggt ggaatcacct cagcatcgga
     9601 ttgatttcct gcctgatgtg caagaggttt ccgaaattgg gctggaacat ctgcttcatg
     9661 cccgcgaggc gattatcagt ttcggtcagg gatgtgaggg cgaaccctca ttaaatgcag
     9721 accgattggc ggagtccatc cgctccatcc gccgggaaac ggatcggggt actattaata
     9781 tgaacagcaa cgccggtttt acccagggaa tcataaaaat gaccgatgcc ggtctggatg
     9841 cactgcgggt cacgattttt tcctgccagg agaagaacta caatatttat caccggccca
     9901 ggaattatcg tttggaggat gttaaaacat ccatacaata tgctcataac cacggtctca
     9961 gagtttccct caacctgttg acgtttcctg gttttaccga ccgggaagat gaaatagaag
    10021 cactcttgca gctaatggct cagggccatg tggatatgct gcaactgcgc aacctgaata
    10081 ttgatcctga ggaattgttt tgccgttttg aatcaggcgg tgaaattttg ggaattgcca
    10141 attttattga tatcattcac caggaaatgc cccaggtgca aatcggttcg tacacacacc
    10201 cgagcagata atcaagcatg tttgacaact gtttatgaag aataaggaga gaatgttttg
    10261 gaaaacaata aaaaactgat gaacgtacgg gagatgcaag ccagttcagt gggccagcag
    10321 gtgtccggca agttcctgat actggacaaa atctatcgta aaaccaaaga cggcaaggat
    10381 atgtacaacc tcaagctggg tgacggaacc ggagaaatag atgccgtggt atgggaaaat
    10441 tgccggctta ccggcacgct gaccagcggc gaagtgattg ggatcttggg agacatgggc
    10501 aatttttccg gcaggaatca gattacagcc aaaattatca aggttttgga tgaggatccg
    10561 ggcctttata tgaaacaggc agagaatctg gaacagctca aacagcattt tcaagagttg
    10621 gtgaaatcgc tgcgggatcc ccatctgcaa aatttgagcc gaagaatttt cgatccttcg
    10681 acccaggagg tgttttttcg tgccccggcg gccaaaagaa tccaccacaa ttatcccgga
    10741 gggttgctgg agcacagttg tcaggtagcc gatctggctt tgaaaatggc ggattcctat
    10801 gcttatctga acaaagatct attgctgttc ggagccttgg tgcatgacat cggtaaaatc
    10861 atggaatata aaatgggcgc ctcaccccag tacacgaccg aaggcaaact gctgggacac
    10921 atcgtgatgg gcagtgaaat gatcagcatt gaaatcaatc gtttgcggac cgagggttat
    10981 gatttcccgc aaaatctgga atggatgatt aaacatatga ttctgagtca ccacggcagc
    11041 atggaatttg gctcgccggt cattccgctt tttccggaag cttttgtcct gcatgttgcc
    11101 gataatctgg atgccaaaat gtatatattc aagaacaagc tgcaggaaaa caatggagaa
    11161 gaagaatact tcaccaatta tgacacgtat tttgcccagc agtttttcac ttatcgctat
    11221 ccggtttggg acgaaatgaa aaaaacggaa tagcatgtaa acagaatggg cgtcaattga
    11281 atgatggacg tcagatcaat atttatttgt cttttttctt ttttattgat ataatgtttt
    11341 tcgcagtctg tttgattgga ctacatgggt gattgacaga tgatagatat taatgtactg
    11401 aaacgaggtg agtgctttga aagaaaaaat acatcccaaa tacggcataa caaccgctag
    11461 atgtgcatgt ggcaatgaga tggaaatcgg atccaccaaa gaaaatataa aagtggaaat
    11521 ctgctccaaa tgccacccct tctatacggg tgatacccgc agaattgttg ataccaccgg
    11581 tcgggtcgaa agattcaaga aaaagtacgg gttaacttag tcaatagcag ggcattgttc
    11641 ctgctttttt ctttatggca gcagtttggt tttccgggaa gcagacagtc tttccggcaa
    11701 ggaaatcagt aaaaaaactg aagtatggca ggatgagtca ttgagttctg ggaggatagt
    11761 acatgctgga gaaacttgca agtctggaat taaaatataa tgaactcacc gatcaattga
    11821 gcaatccgga agtgattgcc gatcagagca agttccagaa atatgccaaa gcgcacgctg
    11881 agctgaccga tgtggtcatc agttatcagg agtataaaaa agcctgtcag gagttggaag
    11941 aaagccaact gatgctgagg gaagaaagcg atccggagtt taaagagatg ttgacggctg
    12001 aaattcaagc gctgcaggaa aaaattgcag ctgcggaagg gcaattaaaa gtattactcc
    12061 tgccccgtga tccgaatgat gagaaaagcg ttattatgga aatcagagcc ggaactggcg
    12121 gggaagaagc agccttgttt gccggtgatc ttttccggat gtattctcgc tatgcggagg
    12181 aacagggttg gagaatcgaa atcatggatt ctcattatac cgaactggga ggcattaaag
    12241 agctcatatt tgtggttgat ggggttgctg cattcagcaa attgaaatat gaatcgggtg
    12301 tccatcgggt gcagagaatt cccagtacgg aatccggagg cagaattcat acttcggcag
    12361 ccacagtagc cgttttgccg gaagctgagg aagttgacgt agccatcaac gaaaatgagt
    12421 taaaaataga tgtatactgt gccagcggtc ctggcggaca atgtgtcaat accacccaat
    12481 cagcggtgcg tattacccat atgccgacgg gtctggtggt cacttgccag gatgaaaaat
    12541 ctcagcataa aaacaaagcg caggccttgc gcgtgctacg ggctcgtttg caggaaatga
    12601 tggaagcaga aaagaatgag gaaatggctg gtgccaggaa aagccaggtt ggatccggag
    12661 acagaagtga gcggattcgt acgtataatt atccccaggg aagggtaagc gatcatcgga
    12721 ttaatcttac cttgcacaag ctggaccaga ttatgaatgg tcagttggac gagattatcg
    12781 aagcattaat ggccaatgac caggccgaac gattaaaaaa ggtggattaa gtgcaggcag
    12841 tctggagaat aaaagatttg cttcaatgga cgacccggta ttttttggac aggggcatca
    12901 aggaatctcg cctggaggca gagatcctgc tggcccatgt attaaaaaaa gatcgggtct
    12961 atttgtatgc caattatgaa gcgccagtca atccggatga aagagcgctg tataaagagt
    13021 atataaaaag acgcaccaaa tcagaaccga ttgcttatat aaccggtcac aaggaattta
    13081 tgtcgctgga tctgatcgta acacccgatg tacttattcc gcgtccggaa acggaattac
    13141 tggtggaaac tgccataaag gcggcaaaag atctggagaa ggtcagaatc gttgacgttg
    13201 ggaccggttc aggagcaatt gccgtcagtc tggcttatta tctaaaacag gcgcaagtat
    13261 cggcgactga catttccttg ccggctttga aagtcgcctg ccaaaatgcc gagcgtcatc
    13321 aggtgagcat tgattttcat gccggcagtt tgctggaatc atttttcacc cgtccggctg
    13381 gagagccgtc agcccatttt gatatcctag tcgccaatct gccttatgtt ccggaaacag
    13441 atcgggatag cctcgaaccg caggtgcgcg attttgagcc tcatttggct ttgtttgccg
    13501 gcgaggacgg cttggatctt tatcgtcaat tgcttcctca ggcccggcgt gtgctgccgg
    13561 agggagccat tatgctgttg gaaatcgatc ctcgtcaggc cggacttatt gcctctagca
    13621 tgcagggatt ttctgaaatc cgcattattc atgacgctgc cggccgcgcc cggatggtcc
    13681 aagccaggag gagccaatga ttgcaacccg ttatttaaaa gtagatgccc agcatcctga
    13741 tccagagcgt atcagtcagg cagctcaatt gatctgtcag ggcgaattgg tagcgtttcc
    13801 aaccgaaacc gtatacgggt tgggtgcggc tgccctgcaa gccgatgctg ctgccaaaat
    13861 atttgcggcc aagggacgac cggcagaaca tcctttactg gtacacatca gccagatgga
    13921 acaggtcaaa atgctggtaa ccgaaattcc cccggaggca cgtcgactga tggaacactt
    13981 ttggccgggc cccttgtcga tcatcttgcc ggccagggag gaagtgccgg agattgtcag
    14041 aggcggttca agcggagtgg gcctgcgtat gccatcccat ccggtagcca ttgccctgat
    14101 caatgcggcc gggccgttgg ctgcccccag tgccaattta tatggtcgtc ccagtcccac
    14161 ccaggccgat catgtcagga cagatctcga cgggcggatt gcggcggtgc tggatgcagg
    14221 agaaaccggc agcggacttg aatcgaccgt gcttgacctc aatcacaact ttcgcattct
    14281 gcgccgtgga ggaattccgg ttgaaaagat agaagccctt ttggggatta gaattggcct
    14341 gcctgaacca gcagtcgaaa actataatag tccggtccag gtcctcctta atcaaaacag
    14401 aagcgagctg gtccagcgca cggaaggctt gcagcaggca aataaacgag cgggcctggt
    14461 gcgtgtgtcc gagtcaaaac aggattatcc atgccgtttt gcgaaagaat atacattaaa
    14521 gctggatggc ctgggacaat ctttcttcac gattctcaga gacgcggaac aagagcaaat
    14581 agacatcctt ttatttgagc ccctggacga aagcaacaac cgactgagtg aggctatgct
    14641 ggatcggatc aatcgggcag ttttccgaaa ataatgaagg agtatgtacc aatgtacgaa
    14701 caaatgggga caatcattct ggtggcaatc gtacttggag ccgacgcttt ttctctctcc
    14761 atgggcatgg gaatcaaagg tgccagtcgt cgttacgcaa ttcgtttttc ccttctggta
    14821 ggtctgtttc acgttctcat gcccttgctg ggcttgagtc tgggtctggc tgccggcaaa
    14881 gtgctgggcg tatgggcagg gcgcattgga gctgttgttt tggtatatat cggtctggaa
    14941 atgatatgga agtcatatcg ggaattgcag ccacgggtaa ttactttggc caaagccagg
    15001 gcatcgttgg atcgcagtga acaagcaatg agcgctgata aatggccaag tttgctgata
    15061 ttgaccgtct ccgtgagcat cgatgccctt accgtaggtt ttagtctggg aacgatcaaa
    15121 gccccgatcc tgttgacggt agtgattatg gggatgatag ccgggtcaat gaccctgctg
    15181 gggttcaaag gcggcaaaat actgagccgc tctgtgggca gttactccca ggcggcaggg
    15241 gggctgatcc tgctggctct ggcggggaaa atggccttct gattagtttt acataaaaca
    15301 gattcttgta gcgttggaag ggaggaaaac gcatgcatat tctgtttgta tgcagcggca
    15361 atacttgccg cagtcccatg gcgcaggctc tcttcaccga tcatgtaaaa aatacaggta
    15421 aaaacaggca agagagtcaa ccgatcgcca agtcggcggg tcttcgtgcc ttggatggtt
    15481 cacccgccag tcccgcggct gtcatggtga tgacggaaat gggccttaat ataagcaacc
    15541 accgttcccg gaggctcaat acggaactgg ttgaatgggc ggatgtgatt ttgaccatgc
    15601 aagaaagtca tcgtcgtgag gttatggaaa aatataatgt gcccgcccaa aaggtttata
    15661 cattggcagc gttcagccgg gattcggcag gcgatattct tgaccccttc ggttttgaaa
    15721 tcgaaaccta ccgacaatgt gcccggcaaa taaatagcat gatcagaaat attgtgaaat
    15781 cattcagcta aaataataaa tatgtgtata ataatttcat tagcattaaa aaagaaagca
    15841 ggagtgaatg acaattggag atcgcaatcg gcagtgacca tgccggggta gagaaaaaag
    15901 aagaaatcat tgcattgctt aataaatcag ggtttgagac gattgattgc ggctgtttta
    15961 cgacggcttc tgttgattat cctgacattg cagaagcagt agcggcgcaa gtgcgggaga
    16021 aaaatatacc gggtattgtg atttgcggaa cgggaatcgg tatatcgatt gcggccaata
    16081 aaatccccgg aattcgagca gcgttgtgtt cagatacctt tacggcggaa atggcccgaa
    16141 agcacaatga tgccaatgtt cttgccatgg gggccagagt tctggagatg ccggttatgg
    16201 aagccatcgt caatactttt ttgacgactt cattcgaagg aggcagacat caacgcagag
    16261 ttgacaagat aaatgccatt gaaaatacgt attgtgagag gtgcaaatga tggactatat
    16321 tgaaaaatat gttttgccgg cagacccgga agttgctgcg gcggttgccg ctgaagaacg
    16381 cagacaggat cgcaagctgg aactgattgc ttcggagaat tttgtttccc gggcagtaat
    16441 ggcggcccaa ggttcggtca tgaccaataa atatgccgag gggtatccgg ggaaaagata
    16501 ttacggcgga tgcgaatatg ttgatgtagt tgaagagctc gcccgggaac ggctaaaaaa
    16561 gatttttggc tgtgaccacg tcaacgtaca gcctcatgca ggtgctcaag ccaatacagc
    16621 tgtttatttt gccgctctca gccccggtga taaagtgctc ggcatgaatc ttaaccacgg
    16681 tggacatttg actcacggca gcaaggtaaa tatatccggc aagtattatc aattttatga
    16741 atacggtgtt aatgcggaaa cagaaatgat tgattatgaa gcattgcgtc ttgttgccct
    16801 ggaaacccgt cctaaaatga ttgtggctgg tgccagtgcg tatccgcgcg tgatagactt
    16861 tgcaaaattc cgggaaatcg cagacgaaat tggggccctt ctttttgtgg atatggccca
    16921 catcgcaggc ttggtcgctg ccggtctgca tcccagtccc gtaccttatg cggactttgt
    16981 taccaccact acccacaaga cactgcgggg accgcgcggc ggtgtcatcc tctgcaaaga
    17041 ggaatgggct aaaaaaatcg acagtgcagt ttttcccggc atccagggag gcccgttgat
    17101 gcacgtaatt gcggccaagg cagtctgttt tcaggaagcc ttgcagccgg cttttaaaca
    17161 ataccagcag caaattgtgg acaatgctcg agtattggcg caggaattaa ttgcgcatgg
    17221 cttgcggttg gtttccggcg gaaccgacaa tcatcttatg ctggtagacg taagaccgaa
    17281 aaaactgacc ggcaaggaag ctgaaacgat tcttgaatca atcaacatca ccgttaataa
    17341 aaacggcatt ccttttgatc ctgaaaaacc taccattacc agcgggatca gaatcggtac
    17401 accggctttg accagtcgcg gcttttcaac cgaggatatg caggctgtcg cccacgcaat
    17461 cagcctggcc ctggatcacc ctgaggatga agccaaactg ggtgaggcgc gggaaatcgt
    17521 tcaggtgctt tgtgataaat atccgctgta tggaatatca ttatgagtaa acaatcaagc
    17581 atccttagaa agcgtcctga ctgggatcaa tactttttag aactggccga tctggttgcc
    17641 agtcgctcca cctgtctgcg tcgacaggta ggtgctgttt tggtgaggaa agaaagaatt
    17701 atttccaccg gatacaatgg tgccccgcgc ggtctgggac actgtctgga gttggggtgt
    17761 ctgcgggaag agatgcaaat tccttccggg cagcgctatg aattatgtcg cggtgttcat
    17821 gctgaacaga atgccatcat caatgctgct ttttatggtg tatccacaca agactcggtg
    17881 atttactgta ccaatcaacc gtgtctgatc tgtgcccgca tgatcatcaa cgcagggatc
    17941 attaaagttg tccatcgcgg taattttgat gaccaattgg ctgtccagtt tttgcaggag
    18001 gccggcattg aacttgttga aataccgcca accattgaaa ggaagtcatg aaatggatac
    18061 caaaacattg tccttgccca gattgaatgg cttaagtccg accctggaat ccaccgccct
    18121 caaattaatg gaggaagccg gggaattggc tcaatgcatt ggcaaatttc gaggcctgag
    18181 cggggaaaca gtacatatgg aagaaagtca tgtcatcgaa gaaatcgccc gggaactgct
    18241 ggatgttgct caaactgcaa taaccatgat gtttgtgctg gaagaccatt atcaaatcga
    18301 tatcgaacag atgttgcaaa atcatgtgga aaaacttgac cgcaaaggtt atctgtccct
    18361 ctaataaaag ccggcaaatt atccgcaaaa atataaaaat ataatgatca tagaggaaaa
    18421 ttaaaattta tagcgaatat cataaatgcg agataaattt cgttttaaaa aagctgcctg
    18481 tttgttatcg ggcagctttc gtcgagatgt gaattctggt acgaacaggg tgaaatctgt
    18541 aatcgcaata gaggatgttc ccttcctgaa tcaaaaaaga ccatacaaaa gctcgaaatt
    18601 aaaaaaatat ctgcccaaaa aggattttgg attaattcgt agaatacagt atgtgttgaa
    18661 aatggtaaaa agaacaaggt ggtgcagatg gcagacaaaa agcaaaattg gccgagggca
    18721 cttgcagatg ccatgaattt agccacatct gtggcggcag cgatagcggt cggctatttt
    18781 ggcggcaaat ggctggatgg caagtttgat acgggtcctt ggctgtcaat cggaggtttg
    18841 ctttttggaa tgctaacagc cggcaaaatg atgtgggaga gattgatgat cgaatcccgc
    18901 caaaaacctt caccgggatc cgcagaggat caggattgaa tattttctga ggaaacacaa
    18961 tcaaggtttg aagttttggg tcaggtccgg caaaagaccg acaaaaccac aaataatata
    19021 ttaataggaa ggggggagaa agatgatatt tggtatgtgg gatggtgtct attttcacat
    19081 aggtcctatt ccagtagacc atctggttgt tacacagtgg ggtatcgttg ccgtaattgg
    19141 tttactagcc ttttttgcca cgaaaaacat gcaagtttgt cccaagggtc tgcagaatgc
    19201 ctttgaatgg atcgttgtat ggaccaccga ttttttcact caactcatgg ataaggaaca
    19261 tattgcaaag aagtatgctc cccttctgtc aacattcttt ttgtttatcc ttttcagcaa
    19321 ttacagtggt ctgattccag gagctgccca tattcaagga tttcaaccgc cgactagtaa
    19381 ctggaacgtt accgtcacac tcgctttgat tactgtattt gcagtccaaa gcgcaggttt
    19441 tgccgaacat ggattccatt atctgggaca ctttgtaagt cccaacccgc tgatgttgcc
    19501 gctgaatcta ctggaagaaa taactcaccc gctatccctt accgttcgtc tatttggcaa
    19561 tatttttggt gaggaaatga ttattgctta tatgatgttc atggttccat tcttgattcc
    19621 atcagccctg atggcgctga gtgttctgct gggcgcaatc caggcaatcg ttttcactat
    19681 cctatcggcg agctatatag ccgcagctac gggagagggt cattaaaatt aacataaact
    19741 agggcaatgt ttataaagca ttggcatgat gaaaggaggg gaaagaatgg gtatcgcatt
    19801 aggagctggt ttagccgtat ctattgccgg tctgggtggc ggcgttggaa tgggtgttgc
    19861 tggtggaaag gctattgagg ccatcgccag acagcccgaa gttggagggg acgtaagaac
    19921 ccttatgttt attacctttg cttttattga aactctgacc atttatggtc tgctgatcgc
    19981 atttatgctg gttggcaaaa tgtaagcatt aaaggtatat cgttaaccta ccaagagggg
    20041 cgatagagca aggcaatgtt atagaacaag caaggctcta tcgcccataa tactattagc
    20101 tctgagaatg gaggtagcac atccgtgaaa aggaacaaaa gggaatattt atttggcctc
    20161 cttttagcct ttgttatggt attgggatat gccaccctct gcatggcttc aggtgatgct
    20221 tcagttcctg ttgcagcacc ggaaggaaag ccccctgtat ttggcaactt gtatgttatc
    20281 ggctggtcag cggtcaactt ctttgtattg ctggcgctgc tctacaagtt tgggttcaat
    20341 ccggttaatg caatgctgga acagcgggcc aacaccattg agagtgcaat caaacatgca
    20401 gaagaaataa gggttgaagt agatcaattg agaaaagaag cgcaggccaa tctggctgaa
    20461 tcccgcaaag aggcgcaaga gattgttgcc agagcgacca aagctgcaga tgatgccaaa
    20521 actgaaattg cagcccaagc caaagaagag gccgcggcaa tgaaaaccaa agcgcaggcg
    20581 gaaatcgctt cagcgacgga agccgccaaa gctgaacttc gcaatacggc agcgaccctt
    20641 gccatgatgg ccgccgaaaa agtcttaggc agagccatta cggaagaaga ccacaaaaaa
    20701 atggtcaaag aatttgtaga tgaagcgggc gatttcctat gctaaacaag agcgtcgcta
    20761 ggcgctatgc tgaagcgttt tttggcattg cccgtgaagc cgataagatt gatgcatatc
    20821 aggcggaatt ggaaaaggtc atcagcacta tcgctgaggt tgacggcatg aaagaatacc
    20881 tggaccatct cctggtaccc gccagagaaa agaaggatct ggtccaaaag gtttttggta
    20941 cgcagctttc accgatgacc atgaatttct tgttcatgat tattgacaaa agacgcgaaa
    21001 gctatcttga attcatctat cgtgaatatg tagaaatggc tgatgaatcc cgtaatatca
    21061 agaaagctga actatttgcc gcccgtgagg tgccccagca ggaattggat gcactggagg
    21121 agagactctc cgcttctacc ggcaaaaaag tgatgttgaa gctgacggtt gatccgacgc
    21181 ttctaggtgg agcaaagata cgcatgggtg accagatcgt ggatggcacg gcagttaaaa
    21241 aactgcagat gttgacagag aaactaaaga aagcaaagat aagttaattc gataggggtg
    21301 agcacgtaaa tgagtattag acctgaagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21421 nnnnnnnnna aagataagtt aattcgatag gggtgagcac gtaaatgagt attagacctg
    21481 aagagattag cgccatcctg aaagagcaga tagagcgcta ccaatccgag gtcgaagtca
    21541 gcaatgtcgg ctcagtcatc tatgtgggtg acggtattgc tcgagtctac ggattacagg
    21601 gagctatggc tggcgaattg ttggaattca caggcggaac cttcggaatg gttctgaacc
    21661 tggaagaaga taatgttggt gcggttcttt tgggacctta ttatcatatt aaagaaggcg
    21721 acatcgtaaa agccactgga agaatcatgg aagttccgac gggcaatgcc atgctgggca
    21781 gggttgttaa tgcattggga caacctatcg acggcaaagg acctatcaat acagacaagt
    21841 tccggcctgt tgagcgtgtt gcaccgggtg ttgttacccg tgcgccagtt aacgtaccta
    21901 tgcaaaccgg cttgaaagca attgactcca tggtcccgat cggcagaggc cagcgtgagt
    21961 taatcatcgg tgaccgccaa gttggcaaga ctgccattgc gattgatgcc atcatcaacc
    22021 agagaaccaa aaaagatatg atttgtattt atgtagggat tggccagaaa caatccacga
    22081 ttgccggtat agcaagtaaa cttgaagaaa tgggtgccat ggactatacc atcatcgtcg
    22141 cggcaacggc ttccgaacct gctccgctgc tttacattgc cccctacgct ggggttgcca
    22201 tggcagaaga gtttatggaa agcgaaaaag ccgatgtttt gattatttac gatgatctgt
    22261 ccaagcatgc agttgcgtat cgtgaaatgt cactgttgct cagacgtccg cccggacgtg
    22321 aagcctatcc cggagacgtt ttctatctcc attcccgttt gctggaaaga gcctgcaaac
    22381 ttaatgaaaa ctatggtggc ggttcaatta cggctctgcc gattattgaa acccaggccg
    22441 gtgacgtatc cgcgtatatt ccgaccaatg ttatttccat tactgacggc cagatctatc
    22501 tggaaaccga ccttttctac gctggtcaac gtccagccat caacgccggt ctatccgtca
    22561 gccgcgtagg tggtagcgcc cagaccaagg cgatgaaatc agttgccggg cagttacgtt
    22621 tggatttggc gcagttccgt gaactggctg cctttgccca gttcggttcc gacctggata
    22681 aagcgacgct ggccagactg actcgtggtg aaagagtaac cgaagttctg aaacaaggcc
    22741 aatatgtacc aatggccaca gaagaacagg taattatgat ttttgcgggg gtcaacggtt
    22801 atctcgatga tatgcccaag aacaaggttc aggagtttga aacggacttc ctcaaattca
    22861 tgcgcagtac ttatgctgat gttctggcta cgatacgtga cgccggtaaa cttgatgatg
    22921 caacgcaaga caaattggta aaaggcatac aagaattcaa gtctaccttc gcagtttagg
    22981 tggtgagtgt aaatggcagg tgcaggagta agagaattta agcgaagaat ccgcagcgtc
    23041 aagaacactc aacagattac caaagcgatg aaaatggttg ctgccgctaa attgcgcaag
    23101 gcccaggaac aggctgaaca atccaggcct tataccgaaa ccttgcaggg aaccttggcc
    23161 agattggctt ctttggcaac cgatgttcag catcctttgc tggtgacccg tgatgaagtt
    23221 cgcaaggttg gctatattgt catcactgct gaccggggtc tatgcggagg gtacaatacc
    23281 aacatcatca aagtcgccaa tgatgccatg gcaacggacg agagaaaagt tgaaaatgcg
    23341 gttattgcag tgggcagaaa agcccgcgac ttctatcgca agcgcaaggg attggaagcg
    23401 gaatatacca atttgggaga cacaatcagt tatacggatg ccagggaaat tgctgaatat
    23461 attatcaacg cttatgagaa tgaagagctt gatgaggtgt atctggttta tgccagattt
    23521 gtaaatgtac tgcggcagat tcctacagta gctaagctgt tgccgattga gaaacttgac
    23581 gcagccgaag aaacagtgga aaaatatgtg gaatacattt atgagcccag tgccgaactc
    23641 atccttttga ctttgctgcc cagatatgta ggcagtcaga tataccatgc catgctggag
    23701 ggaaaagcca gcgaacacgg cgcccgaatg acagccatgg gcaacgctac ccagaatgca
    23761 ggagaaatca ttgattcttt gaccctgcag atgaacaaag tcagacaggc ggccataacc
    23821 gacgaaatac ttgatattgt tggaggcgcc gaagctctca ataaaggagg aaagtagaga
    23881 atggcgaatg taacctatgg agaggtaact caggtcatcg gcgcggttgt ggacgtcagg
    23941 ttcaaaccgg gtgagttacc ggatctaatg aacgctatta ccataaaatc agaagaccag
    24001 gatagcagcg ccagggtcat agctgacctc gatgttaccc tcgaggccat gcaattgttg
    24061 ggcaacgaca ccgttcgctg cgttgctttg agtcctactg acggaatcat gagaggcatg
    24121 aaggctacca ataccgctgc cgccattaag attcccgtcg gcgacggatg cctgggccgg
    24181 attctgaatg ttttgggcag agaggtagat aacgctggtc cggtgaatgc ttcggaatat
    24241 tgggaaattc accgtaatcc cccgccactt actgatcagt tgccggctac aaccatgttg
    24301 gaaaccggta ttaaagttgc cgacctgatt tgcccctatg ctaagggagg aaaaattggt
    24361 ctgttcggtg gggccggagt tggcaagacc gttatcattc aagaattaat cagaaatatt
    24421 gcttacgagc atggtggata ttccgtattc accggtgtgg gtgagcgttc ccgtgaaggc
    24481 aatgaccttt ggggtgaaat gaccgagtcc ggcgttattg acaaatgcgc cttgattttt
    24541 ggtcagatga atgagccgcc gggcgcccgc ctgcgggttg ctttgactgg tctgacggtt
    24601 gcggagtatt tccgtgatgt tggcggacaa gacgtgctga tatttattga caatatattc
    24661 cgtttcgccc aggcaggcaa tgaagtgtcc gcgctgttag gacgtatgcc gtccgccgtt
    24721 ggttaccagc ctaccctggc aacggatatg ggtctcttac aggaacgtat tacctcgacc
    24781 cgcaaaggtt ccatcacttc cgcccaagcg gtttatgtac cggccgacga cttgactgac
    24841 ccggcaccgg cgacgacctt tgctcacttg gacgcaacca cgacgctgtc tcgtgccatt
    24901 tccgagttgg ggatttatcc ggcggttgat cctctggatt ccacctcccg gatccttgat
    24961 ccgcagattg ttggtcccaa gcattacaat gcggcccgcg gcgtacagca gattctgcaa
    25021 agatacaaag aattgcagga cattatcgcc attctgggta tggatgaatt gtctgatgaa
    25081 gacaaactga tcgtggcccg tgccagaaag attcaacgtt atctgtccca gcccttccac
    25141 gttgcggaac agtttacggg cagaccgggt atctatgtac cggttgccga gacggttgaa
    25201 tcgtttaccc aggtgctggc aggcaaacat gatgcactgt ctgaagaagc attctacatg
    25261 atgggaaata ttgatacggt attggcaaat gcaaaaagat tggaggggta gtctgtggca
    25321 gacaacacct tcatggttga aattgttaca ccggagcaga ttctctttaa agatgaagtt
    25381 caatttctgg tggttccgga acttaatggt gagctgggta tattgaaaaa ccatgctccc
    25441 atgatagcag cactggatgt tggcgtgatg agatatactg atccggcagg caaagtaaaa
    25501 aaaatagcct taagcggcgg atttatggaa gttatctata atgaagtccg tgtcttggct
    25561 gaaacggctg aacagggatc ggagatcgat attctaagag ccaaggccgc caaagaaaga
    25621 gcggaaaaaa ggctgcaggc aattaacgag aatatcaacc atgcacgagc cgaaatggca
    25681 ttaagaagag ccgttacaag gctcaaagca gcagatgctc ttttaaaaga ataaaatctg
    25741 gttaccaaag ccggaaggat gatttacctt ccggctttgg attataaacc gattggcaca
    25801 cggccaaatt cgtaatatac tataattaaa tttatcacaa aacgtaatat tgcgtgttgt
    25861 gcattactgt gctgaatatg gcatttaaaa gaggttattg gctgtggaca aattgatgtt
    25921 acttaaggca ttggttattg ccataactgt atattatctg atgaaaggca ttctttatct
    25981 ggttctttgg caggtattgt cgaaagcgga ggagaacggt aaggtgagag ctgccaaagc
    26041 ccgcagtatt attatggaac aacggcgcaa aagagatgaa agagaagcgc aactggcgca
    26101 ggaaaggaag gaaaagagac tctattaagt tcctgccggc actcttatgg aattggcgcc
    26161 gggagcaatt ttccagaatg ggtggtttat atgggagatt tacaaattaa atttttaatt
    26221 ttaatcgtat tggtattttt aatcaccgcc ggcttttgta tattgatctg gcagcagacc
    26281 aaaaagtttg agaaaaaggc cttgctagcc aaacaacggc aaaaggaata tgaaaaggaa
    26341 ctgcgtctaa agatccagga agaaaaaaga gcgcaaaagg aaaaagcagc ggtaacatca
    26401 tcgcaagaac cggagaattg attttctaag cgggtgatac ttgcacaaga acggacgaat
    26461 taactgtcat aaagtcaaac ctaaatcaaa tgagtaaacg aaagggcgaa acaagcggag
    26521 aagcctgatg aatgaacgta ccgaacgttc attcatcagg cttcccgtta ttttagaagc
    26581 agttcgcttc atgcagtatt gttgacctcg tcagttgtta tcggggtaca ttagaggatg
    26641 atatttctcc ttgcccggca taaccggaga atattccccg gcaaatgtgt caacaatttg
    26701 ggtagatcac aacgggtaag gggaagggca aatgagaaaa ttatttattt atatttttgt
    26761 atttatttta ctatgttatg taattgaaac agaaattaaa cattcaattg ctgaaccaat
    26821 tgatcatggt tcgccatatt gtctatcttt tacatcaatc ggtgcaattt tgctcgaatc
    26881 cagattggat tgttgggcaa aaataaaaac agccaatcat tttcaggaac tggatgccga
    26941 attaatcatg atattgcaat tattggatct gccttttcaa agatcagcaa ttatgcatga
    27001 acagcaaaca gattatatat cattatttta ccatgcaacc aagaaccaaa cggattactt
    27061 tattcgcctt caaaccgaaa gtcaaaacac ttatttcctt ttaacagcca tttcttccca
    27121 agatgatctt gctttacacc aggatgaaaa aagattaaaa gaacgctatc aaagccagtc
    27181 ttattttcaa tacaaaggaa tgatgaatga acgacccgat caggctgaac gacgtcaaat
    27241 gctgcaggtt gtatgtcaaa gtttgcaggc tcgggcaatc gatcattata gtgcgcaaaa
    27301 catggattgt atggcagcct ttagtccttt attacgggaa aattatgatc aagttaagct
    27361 ggccggagaa aactacaatc tgcaagcggc catccgcaat gacagtgttt taaaccagag
    27421 ttatatttat ttaggtgttc cgttgctgct aaatgattac tagggaggtg gtgattgaaa
    27481 attggaaatg tatattcaag gaagaacaag actaaagggc gaggtggaaa tcagtacatc
    27541 aaaaaatgcc attttgccaa ttattgccgc aactgttttg acggatgaaa aagtggttct
    27601 gaaaagagtt ccccggatca aagacgttga tacaatctgt caaattttgc agcaacttgg
    27661 taaagaaatt caagtaaaag aggattattt agtttttaac agtcgtgtgc tgcaaacaac
    27721 acccccttat gatttggtaa gacaaatgcg ggcttcattt ctgctcatgg gggcattgct
    27781 ttccagatgt ggaaaagtaa gagtttctct gcctggtggt tgtgccatcg gtaatcgccc
    27841 tattgatttg catttaaagg gatttgcagc aatgggagca gaaataaaat ttaatggtgg
    27901 agacgttatt gccaaagcca ataaattaat gggaaatcat atctatcttg attttcccag
    27961 tgtgggggcc actgaaaata taatgatggc tgcatctttg gcggatggaa caacttggat
    28021 tgaaaatgct gccttggaac cggaaatcgt agatttggcc aattttatca acatgatggg
    28081 cggccatgta agtggagcgg ggacaaatgt gattaagatt caaggggttg aattgctttc
    28141 cggtactgag tatacaccta taccggatcg tattgaagca ggtacttatt taatcgctgt
    28201 ggcagctaca gccggagaag cagtcattaa aaatgttatt cctgaacatt taaaatcaat
    28261 aacagctaaa ttggaagaaa gcggtgcggt cattgaagaa agcaattatg ccattcaaat
    28321 caaaagagaa ggcaaaatta aaccggttta tattaaaact ttgccttatc cgggatttcc
    28381 aaccgatatg caggcgcaaa tcatggctta tttaagcttt gcgaacggaa ccagtgtgat
    28441 tagcgagtca gtttttgaga accgttttat gcatgttcaa gaactgctaa ggatgggagc
    28501 cgatatcaga acggaaggaa aatcagccat tatcagaggc gtcaaagagt tgactggtgc
    28561 ccgtgtcaga gccagtgatt taagagctgg tgcagcattg attatagccg ggttaagtgc
    28621 ccagggaaaa accattgtta ctgagaacga gcatgttgaa cggggttatg aggatattga
    28681 caaaaagcta aagcaaatgg gtgctcagat ttatttaaac taggcagaag gataaaacgg
    28741 tgtctgacag ggactgtcaa gatcaccgtt ttattcatgc gaaattttgt agaggcattt
    28801 aacaggggta cttaacccga agttaaggtc ggcaattcca cttccccacg attgttaata
    28861 tcaatcaaac aaccacgaag ttggatttca tcatagttgc tgccaggatt cacgcagatg
    28921 gttcggccga ttttacagat tccccgactg ggatgaacat gaccgtgcaa acttaacaaa
    28981 ggctggtatt tttttatagc ttcagccaca gcggtgctgc ctactgggcc ggtagccagg
    29041 gaatcaccat ccaaaatggg attcaaatct tcatcataaa gcgtatcttg atccagatca
    29101 gtgtcccggg ggggaacatg aatattaaat atacatttat tcatgttttt cacctgggaa
    29161 gccaattttt caattttttg ggccaactct tcttcgctga catcacgggg atatttgaaa
    29221 atggttggat gtccatggct taaagacagt atttcgtatc cttttatttc tactatttta
    29281 tcatccccgt tttgtacata ttgcatttta ttcagatagt catcaatata aaatggatcg
    29341 tcattgccgg gtattactac acagggaatg ccgttctttt taaagcgctc atccatcagc
    29401 ttggcccatt cttcgatttg cttgatctgc agttctttag taattcgttc tacgccgctt
    29461 tcatcaattt ggtcgatctc ctgttcactc aagataatcc agtaaaaacc tgcatcccgg
    29521 agtgtctttt gcaggtccgg cagttctcct tcatttttga ttttaaccgt tgagccgtaa
    29581 tatgtggctt catagtaacc ttccttggtt ttaataatcg gcacgactgc tttgccaaca
    29641 aaatctccgc catagattac cagatctgcc ttgtaaaatg gtccggcgtt ggtaaacttg
    29701 cgaaaagtac tttctgctgc atgagcatca cttgcgtaaa aaattttcat ggtaaacgct
    29761 tcaccccttt tctattctta tttagtcagc cggtatttct aaaaattgaa tgtctatgtt
    29821 tatcccttgt tttgcatagg aagcttttga cagatagtaa ataatgatag gcaaaaccag
    29881 caggataatt gacattacga tcatggtata gggcatgtct cctttaaaga agggaataag
    29941 gagataggcg cagatggaaa cactgatgat cgttgtcaaa atacctaacc aggaaacgac
    30001 gggaagaccc atgaatcttc cccggactac gggtggagct gattcaaaca acagtttgcg
    30061 gcgataaggg aatacgatgc cggcaatacc gagcagaatc caagccagga accaggtgaa
    30121 aatcgtgtag ccgatataag cggtccaggc tggcaaataa acgtccagca acaagaagat
    30181 ttctgcgaca gccagggcta tggtagaagc aatcaccggg gaaccggatt tgggatcaat
    30241 ttgggaagtt ttggccggaa ccagacgatc aaatccccag gcatatatat tacgggttgg
    30301 gccaaagctc ataccggcac aggaaccgaa agtggccata aaaaaggcaa tggcgatcag
    30361 gaaaataaag agaggactct gggtcagaat acccagtaat atcgttgcaa aaggttcata
    30421 agcgccaaag ggataagccg cattgccagt ccctgacagg tacatcaagg cattggtcca
    30481 taagttacca aagattttgt agcttaatcc atagaatatt gcccaggcgc tcattaaaat
    30541 aaccaacgaa ccaaacatgg ccaataactg agacttctgt acactgcgaa cttctccggc
    30601 cagattggcg ccgaatgtag cccccaaagt gttaagtata atgtatgttg aaccggccat
    30661 tatagtagca ccaaagagaa atttattggg atgtccggct tgcacggcca gaggtataac
    30721 atcgttgtaa tttaaaccgc tcaagcgggt gaaattggca gcaaaagtcc cgggatcagc
    30781 cattaaggca gctacaatga aggttagagc cccaaccccg gtgccaatca aggccatcca
    30841 ggacattttc atcatggccc gactgccttt gtagaaaacc atccagataa taaagatcat
    30901 tatcgtgccg agtattgccc ggatatgagg cgtatttaaa aaagatgccc attgcatcca
    30961 catttgattc ccactgacaa tacccatgga agcgaaaaac tcaaccaaag catattttaa
    31021 tgcccaatct gaacataccc ccgtccaaga gatggctgta aaggaaatca tgaaactgga
    31081 cattaagccc agtgatggat gcaggattcg actgatatag atgtattcac cgccttgtcg
    31141 aggcatggct actgaaagca gccagtacag tccggcgatg acaaacattt gtgaaaccgt
    31201 caaaatcgcc caggacatat gtgcaccggg ataaagaaac ggggcccaca tcaaatagac
    31261 gagtgcgtac atgagtcccg ggttaacggt ggaataaacc agcgtgtcat aagggctgat
    31321 ttgtcgagta aaacctacgg cactttgttg aaaaagttcc aaacctcttg ccaagtggca
    31381 tccctcctcg tttttataaa tcatgaataa catccaggga aagtttattt tttcccgagc
    31441 ctactctgca tcgtcgctga ttcggtacat aatactgtct tcccaaccgg ttacgactct
    31501 cacataaccc agtaattcct gatccaagct cggatcgccg gtgtcaacca gcaacgggtg
    31561 gggttgcagg gctaccagtt tgtcacggct ggcaactacc gtaagattgt ctttacccac
    31621 cagacgaata atccgggggc tgatctgctg gttgccgcgg cccaaaatat ggccctgtcc
    31681 gccaatcgcg gtgacgataa tgcgggcctt gccgtgttga ttgatcaggt caaagagctg
    31741 gttttcattg acatccctgg ctatcagctt tcgatcaaaa atcacatcga ctcccaaaag
    31801 ggtgttggga agccctaatc tttccataat actgcgggta gtggtgccgg ggccgatcac
    31861 atagagcgta tccgcatcca ttccggcaat gatttcagca gccataccct caacttctgc
    31921 ctgttcagaa cgggcacccg atttcacgct ttgcaccctg cgttccaccg gcactttcaa
    31981 atacccatac agtctggcac tgacacgccc ttgccgaaag gcttcttcgt cgatatccat
    32041 tacttccgct tcgcgcagat tgaccacctt gccttgtaaa taaagactgg ccacttcacc
    32101 ggcactatgg gggttgattg catacactgc cgaatggatc tttacgccgg caggaatgcc
    32161 cagtaccggg atgctgcttc caatagcgct gaatatattg cgtgcggtgc cgtccccacc
    32221 ggcaaaaagg atcaaatcaa tgcctcgttc tgtcatcttc tgagcggctt tcatggtatc
    32281 ctccggacaa gtttcattac ttgtgatcga tccgatgatt tcaggttcca gaccggcttg
    32341 acgacactca ttcgccccca tctcatctgg atatgccaaa acatgaatat catttttaat
    32401 tttggcaatg atatgcaatg cttcctgggc acgtcgtgga gcatcagatt tggctcccat
    32461 ttcacgggcc ttttttaaaa tttctagacc atcactgccc tttaaaccaa cttttccacc
    32521 aataccagca atcggattaa caattactcc cagcgttttc accctattat caccgctctt
    32581 ttacttaatt ccatctccgt ccaaagacgg agatgggttt ttatacttct acggtttagc
    32641 ttttttcttt aaatattgcc gccacgtaac cgcaattaaa tcggcatcat cgattgcctc
    32701 atgggtaggt cggtgagaag cagctttatg gggggaccgc ttgacgaaat cgggatcttc
    32761 gtaggcttcc ttggaaatct gcctgagcac ttccacatat tcatccagat cttctttatt
    32821 gtaagaatca caaggttcca gcgtgaaagg ctcgggaacg acccagggat gatgagaggt
    32881 ccagtaatgt tcaatgccga agtcaacgat tcgtttattc acatcgtcag ttccaacgcc
    32941 cgtatcttgt ttaagttttt cccaggaata acgtacttgt tccaggcgac gtttgccttt
    33001 ggcataccag ataaccacac ctgggatttc tgcgattttt ttcatgaagt actgattgtt
    33061 taaaaccgag caaatggctg cttcttttaa accttcagcc ccgtgctgca tgatccacat
    33121 gtaggttttc aaaactacat tggtatttcc gaagaaggtt cgtacttttc caatgctttc
    33181 cggatagttg gagttaagaa gatacttatg gccgtcgaat tcgaccctgg gtacgggcag
    33241 gtatggtttt agaaattctt taacgccaat cgctccggta gctggtccca tactgccgtg
    33301 aggggcggaa aaggttttgt gcaaattgaa atggaccaga tcaaaaccgg cttcgcgggc
    33361 acgatgaatc cccagcatac cattggcatt ggcctgatca tacgaacaga gcgcacccac
    33421 tgcatgagca gcttttacgt attcctcaat tctggggttg taaatcccgg tatcttccgg
    33481 attggttatc atgatcccgg cagtacgatc agagagggct gccttcatgg cttccaaatc
    33541 gggatagccg ctttcatccg gcatcagcgt tatgacttta aatcctgcag tattgggggt
    33601 agctgcatca caggggtggg aaaacatcgt ggtgatgatt tccgttctct tcttgtcccc
    33661 ccgggcgcga tgataagacc tgatcatgga agcattggca aacacaccat gtgcgccgcc
    33721 gcccggctgt aagctgaaag catccatacc agagatttct ttgagaaact gctccacctg
    33781 atagtaagtt tccagaatcc cttgcattgt ttcaggacat tgcaggggat gaacatcaac
    33841 gatgccagga tgtcgggcag ccatatgttc ctggattttg ggactgtatt tcatggtaca
    33901 ggtcccttcg ctgattccca aggtcacgtc agtgcccagg gtttcctggg aaagatggta
    33961 atagtggcgg ttgacccgca tttgattgac ttccggcagg gcaggggatt tttttctccg
    34021 caaattcgcg ggaattgctg aaactccgtc gcccacctga ttgacgatat cttgacaagc
    34081 ccggggaacc aatacgcctc tttcccccgg aacactcatt tcaaagatga tgggttcatc
    34141 ccatctggcc tgatggtaac tatctctgag caattttttt tgtcttgcca ttcgctttca
    34201 atcctttcct tgttgataat ggctgttagc aggagattac cccttgcagc gcataaacga
    34261 gtttgtccag gtctttgtgt tgatgtattt cggttacgca atacaatgca ctctggccga
    34321 actgaggaaa ttcttgtgac aagtctttgc cgccaaatat tttaaattgg agcagatgct
    34381 tgttaatttg cgcgactgtt ttaccggtca tgtcaaaatt gaccacaaat tctttaaaag
    34441 aaggggcggc caattgaatt ttcacacccg gcaatgtcgc aattttttgc atggcatact
    34501 ggcagttttg catgatggtc gtgcctactt cctgcattcc tttgggcccc ataagagcca
    34561 gatatacacc tgcggttatc gcccatagcc cggtactggt gccggtaaat tcttttccct
    34621 tatcgcggga gccatacgag gtacggtcga ataagacttc accaaatccc cattcgcctg
    34681 cctgcagggt atctgtcaga ccaaacatca ggtccttgaa ttcagcaata tatttcatat
    34741 catcgtgggt ggcaataaac ccgccctggc atcctcctgc ccccaggtgc agacccagcg
    34801 ggtgataatc tccgcaggta atggtcgctc cataattggc cggggcttcg attacaccta
    34861 acgatatggg gtcagcataa accacaaatt cagcgccggc ggcacgggca atcttgccga
    34921 tttcttctgc ttgggtttcg ataaatccca aataggaggg gttttcaatc ataacggctg
    34981 aaacgtcgtt ggaaatgttt ttcttgagat catttaaatc catcagacca gttttttggt
    35041 caaagccaat cggaatgatc tgcaattccg ctgccggcac agaatcaaga tagttactta
    35101 taatcatgaa aatatcggga ttcatggtgg ccggaaccaa tacctttttg cgattgttaa
    35161 ttcgattggc catacgtaac gaactggctg cagcttgccc cccgtcgtac atcggaatac
    35221 tcaaaacgtc catgtccatt aattcgccca tctggctgca gtattcaaac agggcttgcc
    35281 atttgccatg atcacccatt gattcaccga cataggcggt gagaaattct ccccggccgt
    35341 ttatctcatc acatacagcc ggcacaaaat gctgggcgca accagctccc agaaaagaaa
    35401 gatattcccc gcaggtttta ttcttgccta acaattcttc ggtgtgacga cgtatggagt
    35461 attcatccag gattggctgg ggcaattgca tttcctgact gtattttaag tgttcaggaa
    35521 tttcttcata gagttgcatg gggttggtta cccctacctc tttcatcatg gcagctttga
    35581 tttccggaac ggagttggga atataaggat agacaatcga ttctttatgc atgtagattc
    35641 ctccattcat atcttcttgg atgatcgcac tgtattagga ggctgacatt aatagggagt
    35701 gtacatttgg caccgggtat cagcggataa ctcggcggtc ggcccgtcaa tggttatctc
    35761 ttgagccttg caaatgtcgt gttcccagta tttgcaatcg tcggcattgc aggtgaccag
    35821 tggcgtaagg ttgctggcgc tgctttcata caggtggctg gaatcggcga tattaatccc
    35881 cagattgtca tctgccaggg ttgaatctct gagatcaatt cctgcttgcg cactgctttt
    35941 gtggacagat tgtttgttta catcccgcgg ataaaaggta tcacagatgg cggagttgcg
    36001 attgttttcc acttggattt caccggctgc acataaatca tccgggtgat gaaaggtaca
    36061 gcttttcact ttacatgttg tcatggtttt ttccaaagat ttaccctcct tttttaattt
    36121 gctttctgtt ccggcagatt attctgattg gcaggaccgg ttttatatcg atagttagta
    36181 ttatcaattt aagtagtctt atgctttcca ttcatttcca gaatgcttgt ttttatctga
    36241 caaatagtct ggcctataac tggaaaaact aaagaggctt ttaaaccaaa caggacgaag
    36301 cgttcataag gaggcaataa agaatggaga tatccagcca aaggttctgg attaagaatc
    36361 aacaaagaat ggataagaat gaattgctgc ctgattttat agaaaaaggt gtatcgcttt
    36421 atgaagtttt gagagtcata caaggcgtac cgcttttttt ggaatgccac ctggcaagac
    36481 taaagcattc tgcacaactc acccagctgg agctgccatt ggatgatcat cagatcagcc
    36541 aacgattact ggcgttaatc aacagcaacc aggtggaaca gggtaatatt aaatttgtcc
    36601 ttaattatcc tgcggctggt caaccggctg atttttatgc ttatttcgta gagcatgatt
    36661 atccttcccc ggaggactac tgtcagggag tcaaaacggt actggttcag atggaaagac
    36721 ccaaccccaa tgccaagata gaccgggcag attatcgtaa aagaattgat gaagccaaac
    36781 gcaagacggg ttcgtatgag gctttgctgg tggatcgaga aaggaacgtg agcgaaggcg
    36841 gacggtccaa tttattcatg ataaaagacg agatggttta tactgccccg gcaaacaagg
    36901 tgttaaaagg aataacccga aaaatggttc tggttgcgtg tgaaaatcga ggttatcaag
    36961 tttgtgaaaa agaaatcagc ctggatgaga tgctggccat ggatgcgatt tttatctctt
    37021 ccacttcgcc caaagtattg ccggtaagac aagtggatga taatgctatt gattccggaa
    37081 aaaacaaggt cgttcaggaa atcatggccg aatatgatgc catcattgac gaatatattg
    37141 aaagacacaa ggctatataa tcagcggagt ttattttaaa aaggccggga atgatatgtt
    37201 tcccggcctt ttgagagtat ggttgtcata tatactgatg gacttttaaa atctccaaag
    37261 cgttggcttt gtcttccccg gccaccaaaa caatcggacc ggtacaaccc ataccgctgg
    37321 aggcaaagat gttgtttttc cataacgttt taacggcgtc ttccaactcc aggatatcga
    37381 tccccgtaat ttgttcagca gtaatcttgg cagggggagg gtttatttct tccttggctg
    37441 gcacggtgac tggagaggca ggaattttcc agccggcctg ttgggctttg gttatttcct
    37501 ggcttgcgac ttgtttcaac ccgcccctga ccatggcagc gcagtaattg atggccccgg
    37561 caataaccgg tacaccggaa gcccgcgaga tgatacaaat cagccggtca aagttctcgc
    37621 cgatgccagg accatatcca tagcccctgg tttcatagtc accaccgctg gtaaaggaac
    37681 tgaagagttt catcagaata ttcccggtca gggtatcaca aactaccaca tcgacactac
    37741 ccagaatcaa atcattgcct cgtaaaatct gaccgccgtc tgcccgttgt gatgtgcccc
    37801 agcaaatatc atagcctttg tcttgcatgg cattcagata tctttccacc tgacgacttc
    37861 cctcaacatt taaaatcccc agggtaggat tggcgattcc gtctgcccgg gcggcagcta
    37921 ttcccaatac agcatttttt accatcgctt ggatacggtc agtgtcactc gttccggttg
    37981 tgctggcgat atacatattt cgaccccggg ccggcgttat gactttgcca atggttgcta
    38041 ccccgatggg aaaaggataa tgcatggtga ctgctccgtc caaatcctta tcatcgagaa
    38101 gtctttccaa taccgcatgc acttcgtctt cgtcaacagc ctcatagacc ggtaaattgg
    38161 actttgggac caaaccgatg agacttactt ccaaaccggg aacttgttgc atggcaattt
    38221 ccgccccgcg aagcaaatcc agcggtgcaa cctcgctgcc gactgttgtt agtccgattt
    38281 ttatttttct ggcggttggg ccgtttcgta acagttccgc tgtttcgctg agaacttcgc
    38341 aaagaagatg catattcctg ttattctcca ttagaaagcc gccccccgct cattgaccgg
    38401 gccaattcct gcatggcatc ggccagcata tttctgattt cctgtttgct ttcctcttta
    38461 ttgtcggatg gcatacaatc cccttgaatg ccctggttgg gctctataat aaaactgatc
    38521 ccatcaaaaa gatccgtcat tctgcccaga aatagactgc ctttgccgat gagcatggcc
    38581 cgctgcattt ttttcgtcag aatattatcc cggatataac caagcagagg tacaccggag
    38641 ggaatatggc cctgggtggg agcaaaacca gtgacaccgt gttggctgac aaatgaggga
    38701 agctcatcac gacttatttc tcccctttta acggccatgg cgccgatcat tttataatta
    38761 gccaagggta catccccagc cccggcggga gatgttatct ctgcattttg caattctggc
    38821 gcataaacgt ccacatcgat tagccgcagt tggacttttt ccaaagggtc aaccacgatt
    38881 gcctggatta ctgcttgtgg tgacgaaccc gaggagattt tatgccggcc cacaatatcc
    38941 gttcggatga taggattcct gccgtcattt tcactgatta gaatcgcata accaccaacc
    39001 atgtcctcca atacgggcat ttccttgttt acatgatccc ggctgttcat gcccagcttg
    39061 gcagaggatc ccccggccat tacgaccaca ttcttaaaaa taccggattg aaccaaagca
    39121 gctgcagtca ggataccatg ggccgggccg gcgcaaaagg ctcgcatatc caccccggtg
    39181 gcattgttgc attcggcagc ttctcctatc gctttggcca ggttgcctcc cccgcgttga
    39241 ttcatatccc ctatggcttc ctcggaggtt tcaataatgt aatctatctg cgctgcgtcc
    39301 agattgtttt tgcgcagcag gtttttcaag gccaaaatcc cggaagcttt ggccaccagg
    39361 ttttcaaaaa caatggtggc acttaagttg ggatcgcttt catgggcttg acgaacacac
    39421 cctaccagtt ggttgttctc atataaaccc tcagcttttc cggagttgac caattcctca
    39481 atataagaac ggctgctggc tttgtttaaa aaacctaatt gtttttcatt aaacattcgt
    39541 tttgcggtta aaatctcttt actgcgggca gcaaacgctt cttccaattc caccaattga
    39601 aaactgtcag ctattttcat tacagcataa aactcatttt cgggaatgat ttccccgtat
    39661 tttccttgtc gatcattgtt gatcaggttt tgataccaag gttttggcat ttcttgtaat
    39721 tcatgcgggg taaggttgcc gataaaaacc tgattagggg catattgaac agcttcttca
    39781 aaactgcgca gagattggga tgctttatga agatactcgg attgcggatt gttttttctt
    39841 tccatcgtga tggtggtacc gtgttccatc ataatattgg gagcatgtac cagaataaat
    39901 ccgactgctt ttatcaccgg aaaattcaac ttgctttcct ccttttatca ttgcgcaaag
    39961 aaatcgtttg gatgcataaa cataactgct atttacgcat ccaaaccggg caggcatgaa
    40021 gcagtacttt aaaagaccgt ttgctcactg atctcagtag tcaatgcctg caaggccttt
    40081 tcgacgatac ttcgtctaag tgcaaactct tcttcctggt ttagttgagg attgcccaaa
    40141 ggataaggaa ttgcgatcgc cggatcaatt cgatttgctc ctacggtcaa ggagatgggt
    40201 acaaccgtac agacatgaac gacaggtacg tcaaccactt cctccagttc ttttaccatc
    40261 gttgccccgc aacgtgtaca ggtccctcag gtggaagtta gaataatccc gtcgacttgt
    40321 gcgtgtttta attcttcgcc gatcgcgtgg gcatattttt tggcattggc gaccgaggtt
    40381 ccattgccga cggttgcata ataaatgtta tgcagttggc cgattatccc ctcctgttcc
    40441 agcttgcgca ttacatccac cggcaatacc cggtcagcat cctggttggc atataccgga
    40501 tcataacccc catgggctgt ttcataagtt tcagcagtca aatcattcat tccgctgata
    40561 tcatatcggc caaattttga ggcactggaa gattcgatgc gatcggggtt gccttttggt
    40621 acgataccgc cggatgttac cagagcaatt ttagcctggc ttaaatgctt aatggccgcc
    40681 cggggagata cccgatcgaa cacaggcatg gggtattctg taacaaaggg tactcccttc
    40741 aattttttaa gcagcatttc cactgcgcgg gctgaacctc tttgctcatg aaaaacattt
    40801 ttgcgaatgc cgcgcgccag gtaaccctct tctgccggag atccaatcac atctccgttc
    40861 gctattcggc ttgccaaccg tgccagcgcc ggtatggcat tacgcatttg cgcagcggaa
    40921 ttgccggttt taacgatata tatatctttt ttatacatgt ctgccccggg attttcttcg
    40981 tacataccag taactaccgg aatctgaagc tttttttgta cggcagtaca taccgcccca
    41041 caagccaagc cgtatctgcc agcattaaag gccggtccgg cgataagcag atcagggttg
    41101 aaaccagcga ctaagccaac gatttcttta gtagcaatat caatattttc gttaaaatac
    41161 gtgtcaccgc agattaccgt acctacgatt gtcatatctt tatccaaggc atttaccagt
    41221 gccgtccctg gtccaaccgg accgtttttg caaaaaggta tggtatcagc tttttcttct
    41281 ccgccaattt gaccaaaaaa ctgatttaaa taatggacta ctctaatggc cacgactgct
    41341 tccccctttt ctaacaaaat tgctgcctta tgttttccat ttcttttttt atctcatcta
    41401 catcgagaac catttccatc atgctgatct ggtcctcata tattgcagga tcaatcaaat
    41461 cttttatttc cagaatgtga taaaccggca gttttagagc gatattggtc agcggtccgg
    41521 cataggtggg atccccgttg atgacggttt cagccgtcag tccggctgat tctgcttccg
    41581 ctccgcccag cacaacaact actttatcgg ttccgaattt gtccgccgtt tccttgattc
    41641 ttagttgatt ggccatgtcc atggccccgg ccgctgttca aacgaaacat tccgtactgg
    41701 taaaaagaat ttccccccct acggtttcca tgcactcggc gatagccgga ccgggaatcc
    41761 cgtcgcgatc acctagaata caaatttttt tatctgtaaa aaagctttta tccattcggg
    41821 ttatccgtcc tttcattttt aagatacatt ttattattag tattccaatg ctcccatgcg
    41881 attcaatccc agttcattgg tagcgccggt aatagcttgg atttcaatag ttaatgagcc
    41941 gtcttgttgt agagacccat caaaacctcc ggcaataatt tggctgggtt caatataacc
    42001 aattaccctg gacatggatg gcaaatgaat caaagcgttg gcattgccgc cgctgactac
    42061 cgcattggcc agcgggttgg catctgccaa agactgggag gcaccatcgc gaccagcgta
    42121 ttcatcggta attagcaccg ttttaatgcc taaagattca agttttctgc aattcatgat
    42181 taaatcggca tcgggattgc caaaaccctc ttcgctgata atggctccgt ctgctcccag
    42241 actctctacc agtttggctg taaagttgga gctgcgttcc ttgtcaagca gggttacatt
    42301 ttcattggtt accactatcc ctatgaagtt caggtctttc ccatggcggg ccaaaagatc
    42361 ggcgatgacc ggattgttga ggtgatgata agtcgtattt ttatcacagg cagaaacaca
    42421 attaccgctt atgatggccc cgtccatcac ctcggtagga tagagaaaag tcggcaaaat
    42481 tctttttgca tccagaccgt acagataggt gtcatgcatt agaccttgcg tttgcagcat
    42541 ataaacataa actattttcg gcaaatccaa atattgtttt atctgctcga gcagaggttt
    42601 ggtttcaaaa agctgggttt ggtcaggcac tgcatttttg cctaattcgg ctacataacg
    42661 ggtagctttc aatccagcca tgcggatggc ttcttcgtgt tcgtgctggg gcaggccttc
    42721 tgctttatca cagagaatca ccagattaat ggtttgggag aaaggcgtat aatcagcacc
    42781 aggtcccgac atatcaatga tgccttcttg aaaacccaca attttcccaa ccgtcagtat
    42841 ggtcatgcct cttaatacat tggttttgcc gctgccagcc atttccgcat tgccaaaaag
    42901 gccgggaaag acctgaccgg ggccatccac tttaactctt ggttccagga cgtccttgac
    42961 cgggcaaatt ctaacttcat cccccgggtg tacaatatca gcttcgattc tggccaggcg
    43021 atcgtctttg attgtattca gcagttccag ccggtcaata taaacaatcc cgttttcaat
    43081 tctactttta tcaccaaatt ggacatcttt gaccaggata tttctcagtt ccaattgcat
    43141 acggctgcct ccttcctact caacatattg tttaagtttg gcctcaatgt cttctggagc
    43201 aatttcctgg ccggacagtt catctatctt ctgaccatct ttataaaaga gtatggcggg
    43261 cagacccaat actttttgac ttatggccag gcgtcggttg ccggaggtat ctaccgaaca
    43321 aaacttgatt tgtggatatt ttggggccag atcttcaacg tggggcatca attccaaaca
    43381 acgttcacat ttgggccccc accagtccac cattacaatt ccgctggcgc caagcacttc
    43441 gttatcaaag ttgtccttgt ttactgcaat cattgctcaa cactccctta catatttttg
    43501 atagcgttta ggctttcctc atttatttct gctacttcct gctttatttc accattcata
    43561 aaaacatgaa gtttgggcag ctgatccggg gtaatatgat accgtctgcc aaccaattca
    43621 ttgcgagtcg catctacttt aaccagggaa aaacgttctt ggttggcccg tacaaaacga
    43681 tccacgatcc caattttttc ggtgccgctc atatcggcag gattataata agcaaccaga
    43741 accggctttt tcgcttcgat aacttggttt ttccacaatt ccgtttcatg cacatatttt
    43801 tctgcagcta cagctgcgat cgcaccatcg ctggcagcag ttaccacttg ccgcaggtat
    43861 ttggctctga catcaccggc tgcaaacaat ccctccagac tggtcgccat tttttcatcg
    43921 gtaataatgt agccctgggg gtttaattgc acttctgccg ggaaaatatc ggtcagcgga
    43981 accgtgccaa taaaaataaa cacgccattc accgggtaat cgctttcttc cccggtttgg
    44041 atatttttca gggtaatact ttccaccaat tcatccccat tgacactttt tactatggaa
    44101 ttccaaaccc attttatttt tggattttgt tgtgcccgtt caaaagaaac ttgattggca
    44161 tccactttac cttcttcatg aatgacgatg atactgacct gatcagcgaa tttgctcaga
    44221 tattcagctt cttcgatcgc agcgtcaccg ctgcctacta cggcgacctc caattcttca
    44281 aacaggtcgg catcacaagt cgcacaataa ctgactccct tgccgcgcag tttgccttct
    44341 cccggaacca ttaacatgcg tggctcagca ccaagggcat aaaccagtgc tttccccaga
    44401 tattctcctt ttttgcccgt gacttttttg gggaaggatt ttacatccag cgatacgata
    44461 ctgtctttaa tcaattcggc accaaatgat tgtgcatgct cggccatggc ctgggtaagt
    44521 cccggcccgg ttgtcccccg gccaaaccca gggtaattct ccaattcttc agtcgttgct
    44581 gcttgaccgc caggtcggcc tttttcgata atggcagtat gcattcgcgc ccgggctcca
    44641 tacattgccg ctgccagccc ggccgggcca ccgcccagga ccaacaaatc ataaattgtt
    44701 tccattttca aacccgcctt tccgttaatg atggaagtgc ttgatcaagc aacgctttgt
    44761 caatcatctg gaaatcctca tccaattggg gaatgattaa gttgtcccgg cgatttgtat
    44821 tttcggcttc cattctttcc agtccgtatg tcaacatgtt tagggctgca taatcaactt
    44881 ccgttgccaa tgcagttaat accattgaac ggtaaggatt atctgcaggc ttgatgcttc
    44941 cggccaatgg atgagacaaa agacgatgcc cgccagctgc tttatttaaa gccgcgtaga
    45001 aaacctgacg ttgatttcca ataacaaatt ggatttcagc attagggata ttttttacga
    45061 ttgggttgtt agttattatt aaaaattgcc cggattgcat tgttaaaatc ctccaacata
    45121 tttaagaaaa aacagaggat cagtataaac cttatcccct gtttgtttac ctgagagatt
    45181 taccagcaaa gacgtgcttc tttattcatt tataaattag tcatgaattc tttattttat
    45241 tccttcctta caatttaatt ggataccata ctttatgaat ttcaatgatg tcaaccgcta
    45301 aaaacaagga tagaacaagg tcggttaaat tcccccggta tagctgaagg ggagcgatca
    45361 tatgatggat tatgaaaaaa aaattattga tcacagggtt gctgtttgta tttatcttgt
    45421 cgatttggcg ttatacggtg accaaaccgg aacccgttcc cgaaccattt gtcaagctgt
    45481 atctgcacca aaaaaacgag gtagtagatt tacaaatgga aacgtatatt attggcacgg
    45541 ttgcggctga gatgcctgca tcttttggca tggaagcact gaaagcccag gctgtttgcg
    45601 cccgtaccta cgcagtcaaa aaaatttctg aaaaacatcc ttatccccgc caggccgatc
    45661 tctctgatga catcaactgc tgccaggctt tctgtgacct cgcggagcat catctcaatc
    45721 cagaaaatct tgcgcgcata aaaactgccg tgcaagagac ccgcggggaa attatgctgt
    45781 atgattcccg cccgatcgat gcgctgtatc attcctgctg tggagggaga acagacagcg
    45841 catggggaag ttcaagtcag cttgcctatt taaaaccggt caaatgcaac cattgtcaag
    45901 aatccaggca tttttacgag aaacatcagt ttcccaacca ccaactggca gccttggggg
    45961 gagataacaa acaaagtctg gaaatcaaga ttctctcctt cacgccgggc ggtcgggcca
    46021 accaaatcag catcaacgga aaaaagatat gggcatcgga actgcggcgt cagttcaatc
    46081 ttccttcgca gtggctgtct ttcaaggtgg ggcgtcaaca aacagaaatc acaactcgcg
    46141 gctacggtca cggtataggg ctatgtcagt atggagcgaa cgggatggcc aagaaaggat
    46201 atgattatcg agagatatta aatcattact accaggatat aaaaatttac agactgcctt
    46261 attaaatgga cgcaggcatt gataacagca acaccaagcc aaggggattc atggcatcgt
    46321 ttgagatcct cccgaatata ttgaaaacaa aagagagggg ggcaatcatg cagaactata
    46381 ttcgcaagag agcagttaaa ttagcccggc atatcgtaca aacatcggat actgtccgtg
    46441 caacggctaa cgcatttgga atatcaaaaa gcacggttca taaggatgtc acggaaaggc
    46501 tgcctagaat cagcgaggaa ttgtttcttc aggttaagca tattttggac aagaataaat
    46561 ctgaaagaca tttgcggggc ggagaggcaa cgcgcaaaaa atatgcgaaa gcagcggaat
    46621 ttgaagttct ataaaataaa atagccagaa acgctaagaa aaggtagaat tataaataac
    46681 ataattttac cggggggcta cgaatttgtg ttttgttgaa gacatcggca ttgacttggg
    46741 aaccgcaagc gtattggtct ttaaaaaagg agaaggaatc gtccttcatg aaccatccgt
    46801 tgtagccatc gacaaaaata ccaataaagt aattgccgtt ggagaagaag cgcgggaaat
    46861 gctgggcaga acgccagggt atatcacagc tatccgtcca cttcgggaag gcgtaattgc
    46921 tgactatgat acgacggaaa aaatgttgag ttactttgtg aagcgggtac tggggaacaa
    46981 actgtttatg aaaccaagag ttattgtatg tattccaacc ggtgccacag atgtggaaga
    47041 aagagctgtg cgacaggcga cattggccgc cggtgccaag caggcttaca tcatcgaaga
    47101 accgctggca gcagcattgg gggcgggaat aaatatagcc ggtgcaaccg gcaacatggt
    47161 ggttgatatt gggggcggga cctccgatat tgccgttctg gcattgggag gcattgtctg
    47221 caatacttcg cttcgtgtgg gcggggacaa gtttgacgaa gccttaatcc gttttgtccg
    47281 tcgcgagtac agcctgatga ttggtgaacg aactgccgaa gagataaaaa tgagagtcgg
    47341 taccgcctgg ataacgtcgg aaaatcgaga tcgctacatg gaagtgcgag gccgggattt
    47401 ggtttccggg ttgcccaaaa ccataaatat cagttcccag gaaagctgga tcgcattgga
    47461 agagccggtg tatgcggtca tcgatgcagt gaaaaaagta ctggaaatca cccctccgga
    47521 attagccgcc gatattgttg acaatggtat cgtaatgacc ggaggtggtt ccctgctgga
    47581 aggtttttcg accttgctgt cagtggaaac caatctgccg gttcacgtgg cggatgatgc
    47641 gatttcctgt gttgcaatag gaaccggaaa agcgttgaat gaaatccatg ttctttccaa
    47701 gaacggacga ggtcccaaac gcttggtata ggtttttttt acccgccgga aggcgggttt
    47761 ttctatattg gcagggtcga ttgtcttggg aagactatgg ttttaagcca ttaccatata
    47821 taatcagata tataccatta aaatgagggt tgacatatgg aagtcagaaa atatgcggat
    47881 attttaggag cccgggtgga tctggtgaat acccggcaag ccatggagcg gatcaagagt
    47941 tttattgcca agcgacaatc agcccatgtt attacgctga atgccgagat tgtgtatcag
    48001 gcgcaagaca atccggaatt attaaagatc atcaatggtg ccgatttggt cacccctgac
    48061 gggataggta tcgtctgggc cggtcgtcat ttgggatacg cttttgagga acgagtaagt
    48121 ggcatcgacc tgctggaact gctttgtgaa caagcgccgc agaaaaattg gaaaatatac
    48181 ttgctgggag ctgcaccggg agtcgccgat gaagctgccc gtcaactgac cctgaagtat
    48241 cccggcatcg atatttgtgg tacacagcac ggttatttca ctgcagcaca ccagcagtct
    48301 atcgtggaag tcatccagca gttacagccc gatatcctgt ttgtagggct gggagcacct
    48361 ggacaagaaa tttggattca tcaatttcaa ttggccttgg ctgttccagt ggcaattgga
    48421 gtggggggca gtttcgatgt tgttgccggg ataaaatcca gagctccgca atgggtgatc
    48481 aaagccaact tggagtggtt gtatcgttta ctgaaagaac cccgccgttt aaagagacag
    48541 ctggcactgc ccaggtttgt atgggcggtt ttgcgtcaga agaaaatcaa aaactgatca
    48601 gttgtttggg ggaatcattt tggacaatga caagatattt gccttggata tcggaacaag
    48661 aaagattctc ggcttggtta tgcaaaaggg tgctgacggc tacgaagtgt tggatgtgga
    48721 aatgatcgaa catcaaaccc gggcgatgat ggatggacag atccacgatg ttgaaacagt
    48781 agccaatacg attctgacga taaaaaaacg cctggaaaag cgtttgcaga tacaattgtc
    48841 cagcacagca gttgctgccg ccggacgttc cttgcaaaca acacatggct atgcggtcgg
    48901 aaatcatcct cagcctgctg agcttagccg ggatgaggtt cgtaccctgg agatcgaagc
    48961 agtacagaac gcccaatatg ctgctgcacg ggatgaaaag acccatttgt cgcatgcgga
    49021 atacttttgt gttggttaca gcgtgattca ttattttctc gaagacgaac ccattggcaa
    49081 cctgatcgga caaaaaggga gcagttatgc tgttgaagta attgctactt ttttaccgcg
    49141 ggtcgtggtg gacagcctgc tgtcctcatt gcagcgagcg gggttggagg tttacagtat
    49201 caccctggaa ccaattgctg cgatctccct ggccattccc gccaatatgc gtcttttgaa
    49261 tctggctttg gtggatattg gggcaggcac gtcggatatt gccattgtca aacacggcag
    49321 tatatatgcc tatgccatgg ttccgttggg cggagatgaa ttgagtgaag aactggccag
    49381 tagttgtctg cttgatttta acagcgctga ttggctcaaa tgtcagattg caacgcagga
    49441 tgagctgcat attaaagata ttttaaacaa tgacttgtgt ataaaagcag aggacctaaa
    49501 aaaagaactg gaaccggtta cgcgtgagct ggcacgggaa atagcttccc atatattgca
    49561 attaaacggt aaagcggttg acgcagtact atgtgttgga ggaggcagct tgactcccaa
    49621 tataggacag tttttggcgg aagctttgga attgccgcgg aatcgggtgg gcatacgcag
    49681 tcgtgacaat gtttctcaaa tcaaggggga ttttagttgc ctgcagggcc cgcaaagcct
    49741 gacgccgctg ggaatcgctt ttagtgcctt ggaaaaaccg ccggttccca tgttgaaagt
    49801 atctgtgaac ggaagagaac tggccatatg gaatgctggc gagattacag tggcggatgc
    49861 cttgctcagt tcagggattt ctcttggcaa tgcctatggc aaacccggtc ttggaaaaac
    49921 gttggaggtc aacggcagtt taaaagtctt taagggagaa ataggaacag cttcttccat
    49981 cagattaaac ggaaaagagg ctaattttga tattcccatc atcaatgggg atcagattga
    50041 atttatcaga gggaaagacg gccgtgatgc aaaggtcacc ctgcaagatc tggataccgg
    50101 aggcaaaggc agtgtttata taaacggaga gaaaatggag cttcagccgg taatgactgt
    50161 aaatggtcag ctttggagaa cggatgctga actgccggac cgcagcaagg tggaaataca
    50221 caggaccagc cggctggagc agatcatgag acaatttgga ataaccgaaa cctggttgca
    50281 ggaacagcca taccattttt atttcaacgg aaatctccgg caaggcaagt ggactgcctt
    50341 gcaaataaaa gtcaatggcc agccggcgca actggacgaa acagtttcgt tcggctccta
    50401 tattgaatat cgtatggatc aaaagcggcc ttgtatagcg gatctggttg aactccccga
    50461 caatcatgat tttactgttt atgtcaacgg agaaaaactg accttgcagg gcaagggcta
    50521 tcgtttaatc aagaatgggg cggtggttgc ggctcagaca gaggtggtca ggggagatca
    50581 aattgagttg ctcccggaag aaagtcaact cattctcagc gatatctttc aatctatcaa
    50641 tattgaaaaa caaccccaag ggatcctgca tatcaaagtg aacggagaag aagccggata
    50701 tacaacgcct ttggctgaca actgtgaagt gaccctaact tgggaaaggg cggattaata
    50761 cctgtaatct tggtagtgtt ggtcgtatgg atggcgaatt cgcgattttg gttttttcca
    50821 tcgcccgccg ttcttgtgaa tgcgatggcg ggcatagaat aaaaaggctg ttaacaaaaa
    50881 gaaaaacaat aacagtttta agatccaatg catatgcaaa aattgtccca acaggctcaa
    50941 gcgcggaata tccttggcag cgatcagttc aacattcatc agttgtttac tgttggacag
    51001 gatttccatt ttcccgagca cttgtccttt agtgacgggt gcctcaacct tttcaggtaa
    51061 ctccaggcgg cgggtaaaat gaatcccgtc gctttttgca tagatcgcat taagttcatc
    51121 ctgcagcacc gcttcgactt ctttaaccga accagcttta actctcacag tgccgcaaat
    51181 ctcgcctcgg ggaatcagca tgatggcatc atatttgtcg aaaccatagt ccaacaagtg
    51241 ggtgacatca ctggtttgac cgcctctcag cgggcagccc agcacgactg caatcaacct
    51301 gagatggcct cgttgggcag tggcgatcaa gcagtttttg gcttcagaag tataaccgtt
    51361 tttcaatcca tctgccccgg cataagtcca taacagccgg ttgccattgt atagcggaaa
    51421 agtaccgtcc cgcaacgtat gttgtttgag cgagctataa ttaagtattt ccgtgtgggc
    51481 aagcgtataa cgggccagca aagccaggtc gtaagctgaa ctgtagtgat gttcggccgg
    51541 caacccgctg acattgacga aatgactgtc ctgcatgcca atttccttgg ctttgctgtt
    51601 cattcgggtg acaaatttgg cttcgctgcc gctgattttt tctgccaccg caacactcgc
    51661 atcgttggct gaggccagtg ccatactttt caaaagatca tccaatcgca tggtttctcc
    51721 aacctcaaga taaatgcggc tgccatctgt ttcggaagct ttccggctgg ccgtaaccaa
    51781 ttcggttttg gcaatttggc cctgttcgat ggattccaaa gccaacaaca tcgtcattaa
    51841 tttggttgtg ctggcaggag gcaattgttg atggggcaga tgggatttta acactttgcc
    51901 ggactctgcg tccattaata cataagatgc cgccgataga gaagaaacat caaccgttcc
    51961 ggcagcagaa aaatggctga ccggaagaaa aagatacaga ccaatcaaga gtatggagaa
    52021 taaagatttg catgataaag acatgttatg aactcccaat tttcgctgga ttaacataat
    52081 tattataatc attacccggt tttatgggaa tatctatgtg aataattatt taaaaaactt
    52141 gttttcctga cgtaaatgca gtgaattgaa gaaaactgaa cagaatatta aaacagggta
    52201 ttttataaaa gaaggttgca cgaaaagcca gatagcatta aaatcatagt aaaggtcaaa
    52261 taaagtcaaa agagggtcgg gaacatgaaa aaaagtttag ctgatcgcat tgaacaatat
    52321 ttaaaagtac tgatatcaag aagtgatgat aaagcaattg agatacaaag aatagaattg
    52381 gcagaaacct ttgactgtgt gccttcccag gtgacgtatg tcatcagcac tcgctttacg
    52441 gaaaaagagg gttatatcac ggaaagccgg cggggcggat gcggatacgt gagaatcaga
    52501 caaagccccc cctcctttgg cgaagatgat ttggaatata tcgatttgtt tgcatttctg
    52561 gcagaattaa aacaacgcca attgctgtcc aaccgtgagg cagagctgat taaatacctg
    52621 gtggtcaata cttgtgcgag tttacctggt gaagacaaac ggaaaatcaa cgctgccatg
    52681 gttaaagctt tatataacta ttttgtgtaa atcatctggc cttgatattt gcaagagaaa
    52741 tccaacttga agggagatca tgaacatgta ttgtgaagaa tgcaaacaaa aaccggcggc
    52801 tgttcacctg acccaggttt acaacggcaa aattatacaa tcacatcttt gcgaagaatg
    52861 cgcagcgaaa aagggaggat tcatatttga cccgggcaac aagttttcca ttcccaatct
    52921 tttaagcagt atttttggtg ccagctgtag cattgatccc gatttgacaa cagaaataaa
    52981 aagctgttcc ggttgcggga tgacttttaa tgacattcgc cagaccggca aacttggctg
    53041 cagcaattgt tatcaggtgt ttgcccagga aatggagccg actttgcgta gaattcatgg
    53101 aaatagccag catatcggca aaataccaac caggagcggg gaaaaggtgt tgctgagaaa
    53161 gcaactggat gccttaaaaa cccaattgca ggaagctgta aaccacgaag aatatgaaaa
    53221 agcagctgag ataagggatg gcattaaagc gttggaaagc agactggtgt aaggaggggc
    53281 tgagcgtgag tattgaaaac atattgaatt gcggatcagt caaatggatg gagagccagg
    53341 agggacagga tgacattgtt atcagcagcc gcatccgact ggccaggaat gtcgttgatc
    53401 tgccattccc aatgcgtttg gatcagaaat caggagatga atgcatgcaa cgggtcaagg
    53461 ccgtttgtca ggaagaaatg ccggcggcct tgaatacggt tgagtataca agcctggctc
    53521 aattaagtat cctggatcga ggcatattgg cggagaaaca tttgatcagt cctggccatg
    53581 cagaatccga ttcacctttt ggggcgattt tgctgaaccg tgacggatcg ctggcaacga
    53641 tggtcaatga agaagaccac ttgcgtatcc aatgcttgct gccgggattg cagttggccg
    53701 ccggctatga gatggctcag caaatggatg attttttgga aagcaagctg gaatatgcgt
    53761 ttgatgaacg aaacggttat ctgacctcat gtccgaccaa tgtcggaacc gggttgcggg
    53821 cttccgttat gttgcacctg ccggcgatgc aaatgaccgg tcaaaccggt caaattctcc
    53881 aaaccattgg acaattggga atgacggtca gaggtttgta tggagaaggt acccaggcaa
    53941 cgggcaatta ttttcagatg tccaatcaga tcacgatggg acaaagtgaa gaagaaatct
    54001 gtaatcacct gcaggcagtt acggaacaga tcgttgaaca ggaacacttg ctcagagaga
    54061 gattgaaaat tgatttgaaa tatcaattgg aagataaaat tggccgggca ctgggaatcc
    54121 tgaaccatgc ccgaatcata aattccacag aagctctggc gttgttatcc gatttgcgtt
    54181 tgggaattga tctgggcatc gtgagcggga ttcaactcaa tgctttgaac caattgattg
    54241 tttccatccg accggcgcat ttgcaaaagt gttttgccca ggagcttgac ccgctgcaga
    54301 gggatatcaa gcgagcagag atcattcagg acaagatcaa agggataaaa taacaaaagc
    54361 ttatttagca aggaggaaga atatgtttag aagatttacg caaagagcta gaaatacggt
    54421 tatccacgcc caggaggaag caagagaatt ggggcatcag gccattggta cggaacacat
    54481 tctgttgggt ctgttacggg aaggtgaagg agttggggcc aaagctctta tgaatttggg
    54541 agttgatttg gaaaacgtcc gcgaggagat caagaaatat attggccaaa atgaaaccga
    54601 caaccaggaa gctgccaatg atttgcccat taccccgagg gtcaagaagg tattcaacaa
    54661 tgcttttgat gaagcccgca tgcaaggtgt gaattatgta ggaacagagc atctcctgct
    54721 ggccatcctg cgtgaggagg aaagtgtagc cggtcaggta ttgctttcca tgggagttcg
    54781 gctcgatgat ttgcgggaac aggttattct gcttctgggc ggtgagtttc ccggcatgat
    54841 gaaccaggag cccaatcagg aacccagtgt tccctcctcg ggacaggggc aaaaaattcg
    54901 taaaaccaag agcaagaccc ctaccctgga cggatatagt cgggacttga ccaaggatgc
    54961 gcaggatgga cggctggatc cggtgattgg ccgtgaagat gagatcgaaa gagtgttgca
    55021 gattttatcc cggcggacca aaaacaatcc tgtcctgatt ggagatcccg gcgtgggcaa
    55081 gacagcgatt gtggaaggac tggcgcagcg aatttttgaa aacaaagtac cggaaaacct
    55141 cagccataaa agagtggtct ccctggattt gtcgtcaatg atcgccggaa ccaaatatcg
    55201 gggtgagttt gaagatcgtc tcaccaagat cgtcagcgaa atcaagacgg ccggtgatgt
    55261 gatcattttt atcgacgaac tccataccat tgttggtgcc ggtgcggctg aaggagcgat
    55321 cgatgcggcc aatattctca agccttcgct ggcgcggggt gaattccagt gtattggcgc
    55381 gactacgctg aatgagtatc gcaaacatat tgaaaaggat gctgctcttg aaagaaggtt
    55441 tcagccgatt ctggtcgatg aacccagcat tgaggagagt attgagattt tgaaagggat
    55501 cagagatcgt tatgaggctc atcatggggt gaaaataagt gatgaagcga tagccacagc
    55561 ggttaggctt tccagccgtt atatttctga ccggtttttg ccggacaagg cgattgattt
    55621 gattgatgag gctgcttccc gggtaagatt ggccagtttt attcttccac cggaaatcaa
    55681 ggaattggaa gcccggatgg atgaactgac gaaagaaaag gaagaagcta tcaatgccca
    55741 ggaatatgaa aaagcagccc agttgcgtga tgaggaacaa cgtctgcgag atcaggtcga
    55801 caatatgcgc aaggattggg tgggaaaaag gagtacaact gctggagccg ttggggaaga
    55861 ggagattacc aacattattt cccgctggac cggcatacca gtcagcaaaa tggccatgga
    55921 ggagagcgag cggctgctgc agttggaaga aattctacat ggccgggtga ttggccagga
    55981 tgaagctgtc aaggcagtgg cccgcgccgt gcgcagagcc cgtgccggtc tgaagaatcc
    56041 caaacgccct atcggctcat ttatcttttt agggcccacc ggagttggta aaaccgaact
    56101 ggcccgtgcc ttggcagaag caatgtttac cagtgaggat gccgtcattc gtttggatat
    56161 gtcagagtat atggaaaaac atgcggtgtc ccgtatgatc ggttctccgc ctggttatgt
    56221 gggctatgat gagggtggac aactgacaga aaaagtaaga agaaaaccgt attcggtaat
    56281 cctgttggat gaaattgaaa aagcccatcc tgatgttttc aatattctgc tgcaagtcct
    56341 ggaagatggg cggatgactg acggacaagg acgaacggta gattttagaa ataccattat
    56401 tatcatgacc tccaatgttg gggtggagaa tctgcagcgc aacaaagcca tggggtttac
    56461 ccgggtggtt gacgaagccg aggactacaa ccgaatgaaa gagaatgtca tggaacaatt
    56521 gaaacacacc ttccggccgg aatttgtaaa ccggattgat gaactgattg ttttccaaag
    56581 cctgaccgag gatgagctca agcagattgt tgctctgctg ctcaaggatt tgcgtcagcg
    56641 tctgcaggac agcgggtatt ccctggaaat aagtgaggaa gccagcaaat tgatcttgaa
    56701 ggaaggctat gatccggctt atggtgcccg accgcttaaa cgagcgatcc aaaaattggt
    56761 ggaggacaga gtttcggaag agatcctgaa gaaatcaatc aaaccaggtg acaccatcaa
    56821 acttaatgca gtgcaaggag aaatgacggt aaacggatag aaaaatcaaa tatttttgtt
    56881 ggtattactg ccattttata gaataatgtt gaccattaaa tagataaaga gattataata
    56941 tagagaaaag ggaaaattat ggctaaagca ctgagtaaat ttgtctgccg cgattgtggt
    57001 ttcgagacgg ccaagtggtt gggtaaatgc cccggttgtg gggcatggaa cagcttgttg
    57061 gaagagcgta ctgcacctgc gggtaaatca cgggcgattc gcacgatcga ggcgttggct
    57121 ctaaactcca ttccgcagac gcaggtatac cgtttttcca gcgggatgaa tgaatttgat
    57181 cgcgtgctgg gaggaggaat ggtcccgggt tcgctggttc tgctcggcgg ggatcccggg
    57241 attggcaagt caaccttgct cttgcaggtg gctgataatc tggcacaact gggtcgcaaa
    57301 gtgctctatc tctcaggcga ggaatccccg ttgcagatcc gtctgcgatc ggtgcggctg
    57361 gggatagaaa cagaccggat ttacttgctg aatgagcaga atctcgattt gttggacagc
    57421 tatttgaccg agatcgatcc tgatctggtg attattgatt cgattcagac ggtttactgt
    57481 gagtcattga gctcaattcc cggaagtgtt tcccaattgc gggaatgtac agccaaatta
    57541 atgaccatgg ccaaacacag ccagcgggtg tttatcctgg tgggccatgt taccaaggat
    57601 ggggcattgg ctggaccgaa agttcttgaa cacatggtcg atgttgtcgt ttattttgaa
    57661 ggcgaaaaaa attattcttt tcgtattctg cgcggggtaa agaatcgctt tggtcccacg
    57721 gatgaaatag gattgctgga gatgagcggg cagggtttaa tggaagtttt ggatcctgcc
    57781 cagatattct taaacaccaa tgagccgcct gccgggggat cagctgtggt cgccagttat
    57841 gaaggcagtc gtcctttgct gattgagatt caagccttgg ttgctctttc cgggcccggt
    57901 tatgccagac gcatggcctc cggcgtcgat caaaaccgac tggcgctaat cattgccgta
    57961 ttggaaaaga aatggggcct caatctatct gcctatgatg tttatctcaa agtgaccgga
    58021 ggcgttttta tcaaagatcc atcagtcgat ttagggattg cagcggctat cctgtcgagt
    58081 tttaaagaga taccgctgcc ccgtgacatg gtgtttttag gggaaattgg tctgtccgga
    58141 gatattcgct ccgtcccttt tctcgataac cgcttgaaag aagcaaataa aatgggtttt
    58201 catcaggttg tcctgccttt cgggggagtt aaatccggtt gggaagaaac cggtttgcat
    58261 atacagggta tccgcaacct ggatcaattg ctagaaatca ttatggaggg ataaacttgg
    58321 cagaagatat caatcgaacc aatttccgca agtcattaaa actgcttgcc ccaggaacga
    58381 tttttcgcgt cggcattgaa aatgtcctgc aggccaatac cggaggtttg attgtagtgg
    58441 gagattctcc tgaactaatg agcctggtaa gcggcggttt caagatcgac tgtgagttta
    58501 ccccttcgcg tttgtatgaa ctggccaaaa tggatggagc gatcatcacc aatcatgatg
    58561 caagccggat cattcaggcc aatgcgcaat tggatcccaa ccccaatctt cctacgcggg
    58621 aaacgggtat tcgccatcgt acagcagagc ggatcgccca acaaacaggc atgctggtcg
    58681 tagctatttc ccagaggcga agtgtggtta ccctttatca aggaaatacg gtttttcgtt
    58741 tgcgtgacat ggcttccatt ctggtgaaag ccaaccaggc tttacaaacc ctcgaaaaat
    58801 atcgcaatgt attgatccgt gaactgtccc acttgggagg aatggaattt gaagatgtgg
    58861 taacagttgc agaagtatgc ggggtcttgc atcgttgcat cagagtttta aatattgccg
    58921 aggaaataga gaattacata gcagagttgg gcagtgaggg acgactggtt aaaatgcagc
    58981 ttgatgaatt ggtcgtcaat gttcgagaag aagcgattct cattataaag gattactccc
    59041 atacgccgga taagagtccg caggagttgt taaatacgat tttacgtgca tttgaggaag
    59101 aaatatcaga ttctgttttt attgcccgga ttctttccct gggaacgacc agcagtcatt
    59161 tggaacagat gatttctccc cgcggctatc ggatgctcaa taaaatccca cggataccgc
    59221 tttcgatcat tgataatctg gtggaaaagt tcaatatgct gaaatatatt ttgccggcta
    59281 ccattgagga actggatgat gttgaaggga ttggcgaagt ccgtgcacgg tcgatcaaga
    59341 atggtctgct gcgtatgcag gagcagatta tgccggagta cctggtttag ttttcgctgc
    59401 caatcaaagc aagccaatca aaaaggccca tatcattttg atatgggcct ttattgttga
    59461 gccgctggga attatcttaa attgtaaatg cctaatgctt cagcttcctt gtattcattg
    59521 atgaatacat catagagatt gatatccaat gtattgcata acgcagccgt ataaaaaagc
    59581 aacttgccca actcactgac aaccacttcc cggcattgct gacaaagttg gccgcgcaaa
    59641 tgattatcca aataagattt taattcactt atggattcga tattatcagg taactgtatc
    59701 ttttttgctt ctactgtaac gcatccacag tcggtaactg cttttatgac agaacgattt
    59761 acccggcaag tcgcttcgtt gagtttggag gttacgtcca aaacactatg atggcgaatc
    59821 aggagattat caacggtgtt ctggaactca tcacaaatca ggtccttcat aaagaggttt
    59881 cgcccctttt cattgtggct ttttatcagt atagccctgt tgttgaaact ttgtcaattg
    59941 aggaagtcca tcataattct tgccttgcgg ccgttcttcg tcttatagct ccaacaaata
    60001 ccatcttttc atcatcacct gcagaaatcg tctgagagaa tcatgtttaa aacccccttg
    60061 gggagatcgg tataattttc ctaatttacc cccacaataa tagttggagg tgtatttatg
    60121 tataagcggt tattgttagg tgtattgcta ttattctcct tggttttagg tatgtggatc
    60181 agttctttgg ctgatcaatt cttgcatttg cctgtcctct ggcaggcaac ggttggtgta
    60241 gctgcaacag gaatgctcta tgtactgcta caattgatac tccatatggc cggccatggc
    60301 atcagcaagt ttaaccacgt gctggacaat acttccctgg aaatgctgtt gggcgggttg
    60361 cttggcctta cggcaggttt gttgctggga ttgcttaccg gttacccgct tgccttgctc
    60421 agtgagttgg gggggtatct gacgttaggc atattcatgc tttgtggtta ttgggggttg
    60481 cgaacaggaa tccacagagc gcaggagata tggcagttat tgccgttgag ccggatttca
    60541 cataaaccct tgtcagactc ctgtggcagt tgtaaagtga ttgataccag tgcgattatt
    60601 gatggccgca tctatgatgt tgctttaagc aaatttctgg aaggaaattt gctggttccc
    60661 ttgtttgtag ttgaagaact tcaacatatt gctgattcat cagacagcct ccggcgaggc
    60721 aaggggcgcc gggggctgga actattgtct aaaatgaatc agcacgcgga tataaaaatt
    60781 gatataattg aaacggatat cccggaagaa aaagaagttg acatgaaact catcaagctt
    60841 tgcaagcaaa tgaatgccag catcataacc aatgattata accttaacaa ggtggctgaa
    60901 ttgcagggaa ttactgtctt gaatatcaat gaattgacca atgcagttaa agttgtggtt
    60961 tatccgggag aaaccatgtc gattaatgtt ctcaaggctg ggcgggaacc cggtcaaggg
    61021 gttggctatt tggaagacgg gaccatggtc gtagtagagg atgctcagca tgaactaggc
    61081 aacagcctgg aagtcttggt gacaagtgtc tttcagaccg cagctggtcg tatgatcttt
    61141 acccgcaaga tcaaagaaga agaaacggcg atggaaagca accttgtatt gcccaatgtt
    61201 aaatcgcaag aggtgaattt gtatggctga caacctgagg gttgttattg cggctgccgg
    61261 gcaaggaagc cgtatggggt ccaagaccaa caaacaattt ataatgttga accataaacc
    61321 ggtgattgcc tattcgttgg atttcttcga aaggcataac gcagtggatg aaattgtggt
    61381 tgttaccagt gccagggagc ttgagtattg ccgcgatgaa ataattgcca ggaacaagta
    61441 ccaaaaggtt tcggcggtcc tggccggggg gcaggaaagg caggattccg tatgggcagc
    61501 gctgcaatat ctagcccggg atacggatta cgtagcagtg catgatgggg cgcgcccatt
    61561 gctgtccact gacgtgctga accgactgct ggaagcagcc gaagattggg gagcagccat
    61621 acctggtgta cccagtcgtg atacggtaaa aatgatcaac aaggattcat ttgtgcgcca
    61681 gacactcgat cgaagtgttg tctatagcat tcaaactccc caggtgttta agttcaagga
    61741 attatatact gcttacaagt atgcggaaga agaagacttt tatgcgaccg atgatgccgc
    61801 cctattcgaa aaatacatcg gccaggtaaa agtggtggaa ggcagttatg acaatatcaa
    61861 aattactacg ccccttgacc tcgtttttgt gcagaccctg ctgaaccgga tggattgaca
    61921 gaggggagaa aaagctatgc gaatcgggag cggatatgat gtacatcgtc ttgtggaagg
    61981 tcgaaggctg atcctggggg gagtggagat acctttttcc cgaggtctgc tagggcattc
    62041 agacgcagat gtcctgctgc atgctatctg tgatgctttg ttgggcgctg cggctttggg
    62101 ggacattggg cagcactttc cggatacgag tgaggaattc aaggatattg acagccgggt
    62161 tttgctgtgc agggtcggca acctgctccg agaccgcggc tggaggatcg aaaacattga
    62221 cactatcttg attgcccagg cacccaggct ggctgcctat ctgcccgcca tgtgccgcaa
    62281 tattgcaaag accctgaaca tggaatcgga tcaggtaaat gtcaaagcaa ccactaccga
    62341 agggttgggt tgggaaggtc agggagaggg aattgccgcg caggcagtag tattgattga
    62401 caaaggcaag gattagggag gagacattgt gaaaaaaatc aaggcaagat ttgcacccag
    62461 ccctaccggt ccgctacata tcggcggagc acgttcagcg ctattcaatt atctgtttgc
    62521 tgaacattat ggcggggaaa tggtattacg gatagaagat acggatttgg atcgctccca
    62581 ccgcgaatac gaagaagaaa ttataacgtc actgcagtgg ctgggattaa cctggcagga
    62641 aggggttaat gtcgggggtg aaaatggccc ttatcgccag acggaacgct tggatctgta
    62701 tcgtcagtat gtgcagcaat taatcgaaca aggatctgct tattattgtt tttgcagtca
    62761 ggaagaattg gaagccgaac gacaggaaca atatgcccag ggtttgacgc cccgctacgg
    62821 tggcaaatgc agtcaattgg acaaggagga ggtcaaccgt cggctgcttg ccggtgagcc
    62881 tgccagcata agattcaggg taccacagaa tacgatttat gtagtcaacg atctggtgcg
    62941 gggaacggta agctttgaaa gcggcaacag cggtgatttt attattgtta agtccgatgg
    63001 gattccggtt tataattttg cggtagtcat cgatgatcat ctgatgggag tcagtcatgt
    63061 tattcgtgcg gaggagcatc tatccaacac gccccggcag ttgatgctct atgatgcctt
    63121 gaaattcccg cggccggaat ttgctcatat ttcactaatt ctgggggatg atcaccaaaa
    63181 gatgagcaag cgccatggag ccacttcgct cgtacagtat agagaaaggg gctatctgcc
    63241 tgaagcgctt tttaatttcc tggccttgct gggctggtcg ccggaaggag aaaaggaaat
    63301 tttaagcaag gaagaaatcg taaaaggttt taccttggac cgcgtttcca aaagcccggc
    63361 tgtctttgac atcaataaac tcaactggat gaaccagcag tacataaaac agttatccac
    63421 cccggcgtta atcgatggtt tattgtctta tattcaaaac agccgctatg cagatgattg
    63481 ggccaggctg tctgcagatc gcactgtggt tttggtcgag gcgattcgag aacatttggt
    63541 ttgcctcggt gatgtggagc agcagatgga agttttcttt cagccgcttg cctatgaagc
    63601 ggaggcctgg gaggtattgc aacaggcaca tgtcatgcag gtcttgaaaa gtgttcataa
    63661 cacactgccg gagttttcta agccccagga gatcaagcaa tttatcaaga ccatagtcaa
    63721 aaccacccaa ttaaaaccga aagatgtgtt tatgccattg cgcagtgctt tgagcggaag
    63781 gacccatgga ccggacttgc ctaatcttat ctatgtctgg ggacgggaac aagcgctggc
    63841 gaggttaagg gagacaatga gaaagctggg cgggtgattt gacaggaccg ggaccgtcag
    63901 catataattt taacaaatat aaaacaaatg ctaggaacgg gaagagtaat tcagggactg
    63961 ttggcagaga gagaagacca ccggctgcaa gtcttcttaa ctttgaacct gaaccaaagt
    64021 gcgcccggga gcagatatgt tgaacctgaa gtagacatat ccggcgggtg ccgttaaata
    64081 gagtaagcgg ggtgccgggc actaatcagg gtggaaccgc ggaagcatag cctttcgtct
    64141 ctgaacagag atgaaaggct tttttaaata agcaaacgag gagatcatga aaatgaaagt
    64201 ctataatacc ttaagcggga aaaaggaaga attgcggacg attgcagcca atcaagtcaa
    64261 tatttatgtt tgcggaccaa ccacgtacaa ctttatccat ctgggcaatg cccgtccttt
    64321 ggtggtgttt gatacggtca ggcgttatct ggcctatcgg ggttatacag taaaatacgt
    64381 acaaaacttt actgatgtgg atgataaaat tatcaaccgc gcccggaagg aaaacgatga
    64441 cccgctgcag ttggccaagc gctatataga tgaatatttt gtggatgcag atgccttggg
    64501 aatatgccgg gctgatgtac atccgcgggt gagtgatcat atcccggaga ttattggggt
    64561 cattgaaggc ttgatggcaa aaggatatgc ttatgaggtc aatggagatg tctattatcg
    64621 ggtgaaaaat ttttccggtt atggcaagct gtccggccga tcactggatg aaatgctggc
    64681 cggagcccgg gttgaagtgg atgagcgcaa agaagaagcg gccgactttg ccctgtggaa
    64741 aaaaaccaaa gaaggggaac cgagttggcc gagtccctgg ggagaagggc gcccaggatg
    64801 gcatattgaa tgttccgtca tgtccatgaa gtatctgggg gagacgattg atatccacgg
    64861 cggcggtgct gatctgatat ttccccatca tgaaaatgaa atcgcccaga cagaagccta
    64921 taccggcaaa ccttttgtaa aatactggat gcacaatggt tttatcaccg tgaacagtga
    64981 gaaaatgtcc aaatcgttgg gaaatttttt tattctgcgg gatattctgg aaaaattccc
    65041 tcatgatgta gtgcgctatt acctggtcgc cacccactat cgcagtccgc ttgattttga
    65101 cgatggaaaa ttggaggaag cccgcaaagc cttaagcaga ttgaaaacca ccctcaatct
    65161 ggcggaagaa tttctgttgc accacgatct tgatatggaa aagcaaacac tgccggatac
    65221 tgaaggagaa ctgtcaggta gtgtgaaagc attgcaagaa aaatatataa ccgccatgga
    65281 tgatgatttt aataccgctc aggctattgg tcatctgttt gaaatatccc atctgctcaa
    65341 tagcttcgta gccaatgccg atccaaagtc agtccgcgag caggtacaat tgcagcaagc
    65401 cctgcagata ttccgggatt tgggcgggat tctggggatc tttatggaaa cgaaaaatga
    65461 ggatctagcg caggcagtgg atgcagtcct ggatatatta ctggaagtac gcaactatgc
    65521 ccgccaggag aaaaactacg ggctggccga cagcgtccgc aaatcgctcc agacgatggg
    65581 ggttaaagtt gaagacaaag ccattggtga gagcgtctgc cgttatgaat cggcaccgga
    65641 gatggagttg ctcatgggtc atatcctgca aatgcgccgg gatttgaaaa aacaaaaaaa
    65701 ctttcaacaa gcagattatc tgcgcgatcg tttgaaaacc gccggtatca taatagaaga
    65761 tacaaaggaa ggagcccggt ggaaatttgc tgacgcataa tctggtagat gaaaagacac
    65821 tgagacaata ctcgccgctg gtgctggctt atgttggtga tgcagtattt gaactggctg
    65881 taaggacacg gattgccggg aacggtccgc gccggcttag ggatcttcat cacgaagctg
    65941 ttgccacggt caatgctgac cgtcaagcag ctattgttcg acagatggag gcccaactca
    66001 accaggaaga acttgatatt gtcagaaggg gacgcaatac ccgcagtcat cctcccaaaa
    66061 atgccgatgt tcaatcttat cgaatgagca ccggcttcga agctttactc ggatatctct
    66121 atttaaaagg ggaacatgat cggttggatt acctgctgca taaagcgctg gaagaaatat
    66181 agaaggtagg cagaatctat ctctgctata atgaaagaca atccccccaa ggttaatgaa
    66241 gaaaccggaa ggatgaagac tatgatcata ctgcagccgc aggtgctgat tcccgaatca
    66301 gaactggatg ccaacctgtt tcgcaaactg gaaagatatg cccggacctg ttataaatca
    66361 gaagataaaa tgaaggatgc cggcgacccc cattttttaa agtcaaagct gaccatggga
    66421 catgagtcca tcatcgaaca tgaaaaggcg actgtgatgt ttatcgttga tcgcggtatc
    66481 tcccatgaaa tcgttcgtca tcgcattgcg gcctatagcc aggaaagtac ccgctattgc
    66541 cgttacagtc aaaataaatt cggcaatgaa atcagcgtga ttgagccata cttctttaag
    66601 aatcgttccg atgactatca attgtggaaa gaagcttgtc tgggagcgga aaggaattac
    66661 atggccatgc tggaaagcgg ctgttcagcc caggaggccc gttccattct gcccaattca
    66721 ctgaaaacgg aactggtggt tacatacaac ctgcgtgaat ggcgccattt tttcagactg
    66781 cgggcagata ttgctgcaca tccccaaatg cgacaggcag ccatcccgct gcttttgtac
    66841 tttcaggaaa aaatgccggc tttattcgat gatgttaatt atgatcgcga ttttccagtt
    66901 gaacattatg ctcaaatcat ccagactgat gaagtattca atccgcttgc ataaatgttc
    66961 acgacccaag aaaaaataat cggagatgat ggcagattgt ctgctcaccg acgctggctg
    67021 atcctcttgc tggcggcagc gggattggtg ctgatatttc aatacaaaga atcgctgatt
    67081 gcacgttttg attatcatac actattggaa caggttgctc ttgaacctga atgtaaagtg
    67141 ccccgtttaa tcagttcccg ggataataat tacaatggga ttccggatcc attggatatc
    67201 gttgccggag cacgacagga ggtggagcaa gggacacttt atgacagttc ctactaccag
    67261 ggaggcttcc ctccggaagg cagaggcgct tgtaccgatg ttgtttggcg ggggtttcag
    67321 gctgccggac ttgatcttaa acaaatggtg gacagcgata tccgcaacca gccgcagtta
    67381 tacggtgcaa cgggcaccaa ccccgatcct aatattgatt ttcgcagagt cagtaacttg
    67441 cagatctttt ttcagcagta tgcccagcag ttgaccaccg tcatgatccc gggcaatgcc
    67501 gaaaatctga gcaactggca ggcgggggac atcgttattt ttggtgctcc gcttgagcat
    67561 attggcatta tttctgacaa acgcagccgc aatggggttc ccttactcat ccataatgcc
    67621 ggtccgcgcg ccagtgaagg ccaatatata ttgaactggc ccagtccgct tacccatcac
    67681 taccgctaca ttccctatga tgagcaatga tccagataaa ggagaggtgc aatgcaggaa
    67741 aaactggccg gtgtcaacag catcatggaa gctttgaacg gcagacgcaa aattcataaa
    67801 atatttgtcc aggaaggccg gggaggcaaa cgaattgaag aactgctaaa actggctgaa
    67861 aaacgaggcg tgtattggca gtatgttgac cgccagcggt tggatcaaat gtatacgcag
    67921 gccaatcatc agggaatcat cgcccaggtg gacagctata catatgcatc ggtggaagag
    67981 attctggaaa atgccgcttt atcaggacag gaaccttttg ttctcatcct cgacggaatc
    68041 gaagatccgc agaatctggg ttcgattatc cgaaccgctg aatgtgccgg ggtacacggt
    68101 atcattgttc cccgtcatca tagttcggaa attacgccag ccgtggtccg ttcttccgca
    68161 ggagcagtag aacacatatt attggccatg gaaaccaatc tggtaaacac cattaaatct
    68221 ttaaaaaaaa gaggattttg gatcattgct gccgatatgg acggagaaca ggattacttt
    68281 tgctgccaga tgcccacacc ggtagcattg gtggtgggcg gggaaggaca aggaatccga
    68341 cgtctggtaa aggaaaactg cgatttggta ttgaaaatcc ccatggttgg ggcaattcaa
    68401 tccttgaatg catcggttgc agcgggttta ataatttatg aagtattgcg ccaacgatac
    68461 aaccagcagt agaataacga agcattgctg tcaatagcac aacaagttct agttgacgat
    68521 tgagatttta tgcggctata atatacttgt ccaaaaatca ccgttttgaa caaacaaaca
    68581 taataaattt ggaggcggtt aaatgatcgg ttctgtagct gccaggcagc tctatgtaga
    68641 ctatgtcgag atgtccgacg aggaaatcgt tgaactggca cagttgggga atcagtttgc
    68701 catagagttt ctggttgaca aatacaagaa ctttgtgcgt gccaaagcca ggacctattt
    68761 tttaatcggc gccgaccggg aagatattat tcaggaaggc atgattggat tatttaaagc
    68821 catccgcgat tacaacgggg aaaagttaac ctcctttcga gcttttgcgg aattgtgtat
    68881 tacccggcaa attatcaccg caattaaaac tgccacccgg caaaaacata ttcctttgaa
    68941 ctcatatgtt tcattgaaca aacctgttta tgacgaagaa tccgatcgta ctctgattga
    69001 cattatcacc accaataaaa ttaccaaccc cgaagaaatt atcatcagcc gtgaggaatt
    69061 tatatacatt gaaaaaaaga tgggagaaat tctcagctct ttggaatgga aagtgctgat
    69121 ggcttatctg gagggcaagt cttatcagga aatcgcccag gaattaaaac gccatgtcaa
    69181 atcaattgac aatgccctgc agcgggtcaa gagaaaattg gagaaatacc tggaagaccg
    69241 caacgaaatc aatcgataag attttttaga cgtctttatt tttggtatcc attcaactga
    69301 atcctgcgag taccggaaac ccataaaatt attaatgcgc ttgactttaa tcggctcagc
    69361 atataaaata ttatttgctg agccgagcta gctcaatcgg tagagcagct gatttgtaat
    69421 cagcaggttg cgggttcaag tcctgtgctc ggctccagag aattacatgg agggataccc
    69481 aagcggccaa agggggcaga ctgtaaatct gttgtcgacg acttcgaagg ttcgaatcct
    69541 tctccctcca ccatcatcat catcgcggga tggagcagtt ggtagctcgt cgggctcata
    69601 acccgaaggt tgtcggttca agtccggctc ccgcaaccat gattcgtcat tcccgaaggg
    69661 aattaaaatc agtggcccat gcagggccaa ccatgattcg ttattcccgc caggaaattt
    69721 aaaataggtg gtcccggtag gggcagaaga agagttttat caacatattg cccttatagc
    69781 tcagtcggca gagcgcatcc ttggtaagga tgaggtcacc ggttcaagtc cggttaaggg
    69841 ctccacttgg cggtgtagct cagttggcca gagcattcgg ttcatacccg aagtgtcggg
    69901 ggttcaaatc cctccgccgc taccataata aacacactaa aggagccgga ttatatacct
    69961 gcttctttta ttttgaacaa tttttggtta gaatatttac gtacgcactg ggttatcccc
    70021 atttactatt ataataagga aaggtgagca agagatggca aaggcaaaat ttgaaagaac
    70081 aaaacctcat
//
LOCUS       DAOTHZ010000004        52593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_633_length_52593_cov_7.618448, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000004 DAOTHZ010000000
VERSION     DAOTHZ010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 52593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52593
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_633_length_52593_cov_7.618448"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /gene="plsY"
                     /locus_tag="P5549_01135"
     CDS             complement(<1..441)
                     /gene="plsY"
                     /locus_tag="P5549_01135"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRW11706.1"
                     /translation="MEKIIVVIISYLLGSVPFAYIMIRLFKGIDIRKVGSGNVGSTNA
                     LRTAGKRVALAALLGDLLKGLAAAWIGLQTGGEGLAAVCVLAAVIGHCWPVFIGFKGG
                     KGMATTAGAILLLMPKVFLFLAIIFVLIVFFSRYVSLASVSVAVL"
     gene            complement(472..1797)
                     /gene="der"
                     /locus_tag="P5549_01140"
     CDS             complement(472..1797)
                     /gene="der"
                     /locus_tag="P5549_01140"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRW11707.1"
                     /translation="MSKPIVAIVGRPNVGKSTLFNRLVGGRKAIVEDIPGVTRDRLYD
                     SSEWLGREFIIIDTGGIRFDAGELFAREVKLQAELAIDEADVIIFVVDTKEGITHEDE
                     QVAAILRRSKKPVVVAANKVENFDKQLEYFEFYNLGLGEPIPVSAMHGRNTNDLLDAV
                     IALFAPLSAYKEDPDAIKIAIVGRPNVGKSSFVNALLGQERVIVSEVPGTTRDSIDTS
                     LKYGGNQYILIDTAGIRKKSRIKEPTEKYSVIRSLKSIERADVVIVMLDASEGVNEQD
                     QRIAGYVHEQGKASILVVNKWDLVEKDEHTMNKFDKEIREDLKFMAYSPILYISAVTR
                     KRIFKVLELADFVAGQHVRRITTAELNQVINEAILLNPLPGGGGHKIKILYSTQVGVA
                     PPTFVFFANKPEHIHFSYLRYLENELRKNFGFEGTPIKMILRKRENKES"
     gene            complement(1782..3125)
                     /locus_tag="P5549_01145"
     CDS             complement(1782..3125)
                     /locus_tag="P5549_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRW11708.1"
                     /translation="MAAEIRHVRTDSIGAEMELEAGDLLLSINDHPIYDLLDYQFHSQ
                     EDTLLLEIRKSNGEIWQLEIEKEDDEDLGLEFEGIVFDKMKSCDNHCVFCFVDQLPRR
                     MRRTLYIKDDDYRYSFYYGNFITLTNLTEADWQKIESMHVSPLYISVHCMRGDVRARM
                     LANQRAANIKADLQRLKNANIQIHTQIVLCPGINDGVMLEESIEELAGFHPSVQSVGI
                     VPVGLTGHREKLPVMEPVDTALARRLVELAAGWQKRFRSQVGYGFVYLADEFYLRAGE
                     PLPAAEYYDDYCQLENGIGLSRLLLEEFADLEGQLPEEMKAREVYLLTGQAAIPVWDS
                     VIGRLQQIKGLRIELLPVENHYFGGGVNVTGLLTGSDIIRALGSCFPGKTILLPEIVL
                     RDGQHILLDDMTVDDIMKKSGAQIIPVDGSARDLVEKILNRKLPGSLEDAICQNQ"
     gene            complement(3202..3663)
                     /locus_tag="P5549_01150"
     CDS             complement(3202..3663)
                     /locus_tag="P5549_01150"
                     /inference="COORDINATES: protein motif:HMM:NF022827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HRW11709.1"
                     /translation="MNILFLGSTGVHQALLAARLYLDPGLDRDYKTLPYFNDYQLESG
                     GHPLYIGSDQRNRRVHCLGVGSDVQMVNKTLRQLRIILDSSPEEMEIIPIVIKSQRLI
                     AWLHQLAQIGWLKPLSSNLIACILQLEYENIQQQIPGLTKSDSLYRSKTME"
     gene            complement(3667..4134)
                     /locus_tag="P5549_01155"
     CDS             complement(3667..4134)
                     /locus_tag="P5549_01155"
                     /inference="COORDINATES: protein motif:HMM:NF022827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HRW11710.1"
                     /translation="MKIIYHCFGGSHSSVTAAALHLGLLSRERLPTAAELMALPYYDK
                     TSNGDFGSIHYMGSDEDGNEVYILGKKSLGNRFGCLLRGVAEILGKDNQLLVVNVMDR
                     VNASMKLGGFTSRRIGIPALGRPVVIQGTQQAFEAMCNLVETVKISVLDCRQR"
     gene            complement(4164..4358)
                     /locus_tag="P5549_01160"
     CDS             complement(4164..4358)
                     /locus_tag="P5549_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11711.1"
                     /translation="MNETSLNHCILAIVCRRPEKVSGACPTFIAASQEEQQNICLLLA
                     RILGGIVHDLENGVYIIVKH"
     gene            complement(4358..5248)
                     /locus_tag="P5549_01165"
     CDS             complement(4358..5248)
                     /locus_tag="P5549_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HRW11712.1"
                     /translation="MKAYLVVILVGLCLGFVDRCVMLRNDYRHYPTYPHGHLTHLAMG
                     FIAAGLGAMVVPAISKPDYVAVTFLVLAAQEFRNIRNMERESLGKLDENKLIGRGQDY
                     IEGIAKVFEARNYLCMSTALISSAVTHIWSWPLGVVAGLVCILLSLKWMAGRQLGEVI
                     EISRGKLHFAGSLLMVDDVVIMNVGLPATREKILAEGLGIILHPQDDDARMTLDNIGQ
                     RMAIIYDVVSILGSKIEINETELHPMARKNPDKGYLALFIVPNEPDVESLIDIVKKVP
                     LLESAQGIGLSSLAGRKAAD"
     gene            complement(5250..6311)
                     /locus_tag="P5549_01170"
     CDS             complement(5250..6311)
                     /locus_tag="P5549_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRW11713.1"
                     /translation="MHKKLFALASVMLLFLLITAGLVVAQQELPSNRYFSLIDEDNHV
                     IHQTGTQVYPGDVYIAANNSKFKVVEVSGYTARCVYQGQETLPSLDTARLQSLLEGGI
                     LPAVSKAANNKIAVYHTHSDESYVPSDGKESINGNGGIYDVGEAFVKQLQAQGFKVVY
                     NKNNHNPHDINAYNRSRKTASTLLKEMPAAIIDVHRDATPPGEYQANVKGQDVTKIKL
                     VVGRSNPNSQTNLQFAKQLKAAMDKVSPGLSEGIFIGKGDYNQDLSPRAMLIEVGAHT
                     NSKEEAEKGVKLFAQSLPVVLGMNGSDKTASANPEGPAQKPAGQSDQGSTTTLLILIA
                     VVAIVIGGYFFLNRSSSKG"
     gene            complement(6304..7017)
                     /locus_tag="P5549_01175"
     CDS             complement(6304..7017)
                     /locus_tag="P5549_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HRW11714.1"
                     /translation="MWNIPAGMIALIIVSILIYFGVAHRVLDRMRLNDRTALAVIAAI
                     IVGSFIDIPLGTRITINLGGSIAVILALYVLLGAGTGYEKIRALAAAAITALALYLAG
                     RFLGAEPEQIFIDPIYLYPLIAGIVGYLAGRSRRSAFFAAVIGVFSLDVGQFVYLIST
                     GVRGTVHIGGGGAFDSLILAGILAVLLAELIGESRERIQGGPRTEGRPEELIRNLREP
                     EPARKPQAKSAPERGDEDA"
     gene            complement(7190..8242)
                     /gene="fni"
                     /locus_tag="P5549_01180"
     CDS             complement(7190..8242)
                     /gene="fni"
                     /locus_tag="P5549_01180"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRW11715.1"
                     /translation="MREQRKNEHLQLALQVLDGPEQNGFADIVLIPDAVPELGMDDID
                     LRSHFLGKTLACPIMINAMTGGSGEAKTINRELAALAARYGLAMAVGSQTVAMEDRQL
                     ADSFIVARETNPDGVLIANVSAGCPAEKALQAVEMIAADGLQLHFNLPQELAMQEGDR
                     NFRGLLDNVGEIVSRCPVPVIAKEVGFGLSRESVVKIHARGVHFFDCGGKGGTNFMAI
                     EDQRGGMFDPSFYHWGIATTASLAEIAALQLPIKIIASGGIRSASEAAKAIALGADMV
                     GISGLFLRILLQEGAAELERKTERFLYQLQAVFLMSGAATCREMQQKPLLIFDQTAQW
                     LARRKIDPAQWSERSE"
     gene            complement(8242..8835)
                     /locus_tag="P5549_01185"
     CDS             complement(8242..8835)
                     /locus_tag="P5549_01185"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HRW11716.1"
                     /translation="MHCSGAILAGGKSTRMKFNKAFAVIADKSVYQIIVEKFQQSFED
                     ILTISNEPNLYRQMGTRVCEDVYPGLGPVAGIHSALYHACFDAVFVMGCDMPFMNMEL
                     VAYMLAQLGDYDGVVPQIDGKLQPLSAIYSKKCLPVLTDCLQNDRLKLIRVFEELNAK
                     TIAEQELCQFGNIREIFMNVNDRDQLSLARNIAGRYL"
     gene            complement(8839..9069)
                     /locus_tag="P5549_01190"
     CDS             complement(8839..9069)
                     /locus_tag="P5549_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11717.1"
                     /translation="MDRGWMLDDDDVVNVVEQINLRILNDIAKTYHQREHISIEVKHK
                     GEPIKNLHQLKEDIHSIERIFNVTFNFSPDLR"
     gene            complement(9155..9361)
                     /locus_tag="P5549_01195"
     CDS             complement(9155..9361)
                     /locus_tag="P5549_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRW11718.1"
                     /translation="MPIFDFACNDCASQFDLLVANKDKDKVQCPECGSRNIKQLLSVF
                     ATNSSGNAPAQNSCQSCSSTGSGG"
     gene            complement(9382..11427)
                     /locus_tag="P5549_01200"
     CDS             complement(9382..11427)
                     /locus_tag="P5549_01200"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HRW11719.1"
                     /translation="MQLIVADEAGFCAGVRRAFQISEETALEEGSWFTLGPLVHNKAV
                     VEYFSHKGLASVEDIKDLSGQGIIIRSHGVGPQTIARLEEKGLQIKDATCPLVKKVHA
                     AARLLLNEGYDIIVYGDIHHPEVQGILGWCDHKAQVITSIDEVQSQKRCKKLGIVSQT
                     TRNEEEFLAVVELLIPKAQEVRVFNTICSATRKRQEAARRLSAQVDRMLVIGDRNSSN
                     TKTLAQECRSTGVVTHVIESAQDLDPSWFKASDTVGVSAGASTPDWIIKEVLDRMTQF
                     DDGAPQEQMQESPMETNVPQENEAGEENFAKMEAEMADLASPNRGDVIKGIVVEVHDD
                     DVMVDVGGKSEGIIPLREMSVKDVSSAKLLATVGEEIEVLVLKWDDDGTILLSKKKVD
                     SKRAMDKLEETFNEGGLVEGAIISSVKGGLLVDVGVVAFLPASHVDDGYVKNLDEYIG
                     KTYTFKIIEFNRNKKRGSQIVLSRKQLVEAEKSKAREQFWTAIEEGQVIKGRIKRLVD
                     YGAFIDLGGFEGLLHVSEIDHVRIDHPSAVFQEGDEIEVFILALDREKQRVSLSRKKL
                     LKSPWEIVMEKYTEGDIVDGKVVRIAPFGAFVEIEPGVDGLVHISQLANYRVEKPQDV
                     VAVNQNIKVKVLSIDKDEKRIGLSLKEAAEPVLESEVQEYLQHQDDQESAGEPAAEE"
     gene            complement(11485..12078)
                     /locus_tag="P5549_01205"
     CDS             complement(11485..12078)
                     /locus_tag="P5549_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRW11720.1"
                     /translation="MLYNSARAVLKFIFLIMGLKIEGFANLPGSGAVIVAANHLSFMD
                     PLLVAVAIDRPVHFMAKAELFNQKIVGNLLARVYAFPVKRGNADRHAIRHALQLMEDG
                     KVLGIFPEGTRKKPGQVENTQAGAAMIALRSGAPILPVACIGTDKTLPCGWLRPLMIR
                     IGEPISMLDYQGQKINSETMGKLSNEVSEKINDLLRQ"
     gene            complement(12065..12736)
                     /gene="cmk"
                     /locus_tag="P5549_01210"
     CDS             complement(12065..12736)
                     /gene="cmk"
                     /locus_tag="P5549_01210"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRW11721.1"
                     /translation="MQIAIDGPAGAGKSTAARLIAEKLGYIYIDTGAMYRALTWKALV
                     KKIDLQDEEKLRELAGRTDIRFVKEAEQQQVFCDGVDVSEAIRSPQINANVSVVAAQP
                     RVRNVMVKKQQDMAQMNDVVMDGRDIGEQVLPQADIKFYVTASIEERTRRRLLELEKQ
                     GFIQDETSVRRQIEERDRMDRERAMGALKILDDAFVIDTSHLDIEVVLDRMLAIIGEK
                     QDALQ"
     gene            12945..13388
                     /locus_tag="P5549_01215"
     CDS             12945..13388
                     /locus_tag="P5549_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11722.1"
                     /translation="MSLSMNFQLHYINQEQHLMLPRTSSIILTQNLYDILFQYVITPE
                     KEALLDSFIKRLESHIKSKAEAPFSLPVSELEFLEEGLEELRLLNWAEIPVSVFRINL
                     DACLDKERTEDEMENIATLLAQLMIIRRYPGSDLVYVYPSNLVRY"
     gene            13428..13967
                     /locus_tag="P5549_01220"
     CDS             13428..13967
                     /locus_tag="P5549_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRW11723.1"
                     /translation="MDLKGSRTEENLMKAFAGESQARNKYNFWASQAKKEGYEQIAGF
                     FEETADNEKEHAKRLYKLAIGGSIKSTVENLVEAAQGENEEYTSMYPEMERIAREEGF
                     DEIADVFREIGEVEEAHEKRYLKLLENIKNGTVFKKSSPVKWKCRNCGYIHEGTEPPE
                     LCPACAHPRAYYEELAENY"
     gene            complement(14053..14916)
                     /gene="speB"
                     /locus_tag="P5549_01225"
     CDS             complement(14053..14916)
                     /gene="speB"
                     /locus_tag="P5549_01225"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRW11724.1"
                     /translation="MQNLAWLERKAIFLASRDHLEEADRVIIGLPMDSTTSFRPGTRL
                     APYRIREVSEEVEEYSIYQDLSLEEIAFYDAGDVQIPFGNVQRSLERMEDTARALLAM
                     QKKIFSIGGEHLVSFPIIKAYKEVYPDLVVIQMDAHADLREGYMGEPLSHASVMNHVL
                     QLVGDRNLYQLGIRSGTREELQLAQRCSNLYLDQFLAVIAEVKAKIGRRPVYLTLDID
                     VLDPAFAPGTGTPEAGGVTSRDLLQAILELASLHVVGFDLVEISPPFDIADNTSILGA
                     KILREALLAFR"
     gene            complement(14919..15749)
                     /gene="speE"
                     /locus_tag="P5549_01230"
     CDS             complement(14919..15749)
                     /gene="speE"
                     /locus_tag="P5549_01230"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRW11725.1"
                     /translation="MELWVTEYQTPYLGFSCKASETLRVEKTPFQDLAVVVTEQFGRM
                     LLLDGMVMTTDKDEFVYHEMISMIALYSHPCPRKVLVIGGGDGGALREVLRHPQVEKG
                     VLVEIDAQVIQAARDFFPALSCSFDDPEAEVIVDDGIKYIQEHKDEFDIILIDSTEPV
                     GPAIQLFSPEFYASCYAALKEDGMMVAQSESPFFNQNVIQMVYRGIGQVFPITELYLA
                     NIPTYPSGLWSFTVGSKKYDPRRIQSSPEKPCKYYNAGVHQAAFELPAFVQEIIGQGK
                     "
     gene            complement(15781..16239)
                     /locus_tag="P5549_01235"
     CDS             complement(15781..16239)
                     /locus_tag="P5549_01235"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HRW11726.1"
                     /translation="MLPLPKKFFVTAGSSEGTTALTAFDGALLNARVGNTNLLRVSSI
                     LPPGCEFEPDLVIPPGSLLPIAYGSITSSEPGDMISAAVGVGIKKNSFGVIMEFSGKC
                     SKKEAEEQVVRMVEEAFAMRNLELDEVKVAAVEHTVMNIGCAFAAVPLWY"
     gene            complement(16407..17300)
                     /locus_tag="P5549_01240"
     CDS             complement(16407..17300)
                     /locus_tag="P5549_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HRW11727.1"
                     /translation="MNVNLFKTYIRVVETRNLSRTAEELGLSQPAVTKQIQALEDLYG
                     ILLLERSGRRLKTTEAGETLYSHARDLLRSMDKLEQAMQDIAEDRKGSLLLGASTIPG
                     QYILPALIKGFKEEHPHISISLEIADTEKIFSKIGERELDIGLVGGWLNQRKAEGFPW
                     REDELVAVVPPNHRLARQSRCSLADLQQERWIFREKGSGTRKAVEEMMNAASFNKDQL
                     DVYMEAGSTELVLAMVEAGMGISIVSRWVIRADHPRTGLCYLNLVEKEARRVFYVIYP
                     KQKVRRKSVNAFLDYLKNNPS"
     gene            complement(17322..18248)
                     /locus_tag="P5549_01245"
     CDS             complement(17322..18248)
                     /locus_tag="P5549_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRW11728.1"
                     /translation="MKLNNLDLDLLNSITELHGIPKGAVNIRRDGQAVIRQSSPNISL
                     SSIVGDKPGLLVEIKPGTRNETVHVPVILTQSGFQDKVYNTFIVGEDADATIIAGCGI
                     HNDGHGSSRHDGIHEIIVRRGGKLKYIEKHYGEGGGEGKNVLNPTTVIIVEKDAVVEL
                     EMVQIKGVDDTFRRTEAHVHEKGNLKIVERLLTQGNQQAESDIEIIIEGVGGVAQVLS
                     RAVAQENSYQVFRAALVGKASCVGHVECDAILMDKARIKSIPELMAEDAEAVLTHEAA
                     IGKIAGEQLIKLMTLGLTEKEAVDAILEGFLR"
     gene            complement(18235..18987)
                     /locus_tag="P5549_01250"
     CDS             complement(18235..18987)
                     /locus_tag="P5549_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW11729.1"
                     /translation="MLKLENIWMQIACDSGEDKEILRGVNLDFEKGKVYAITGPNGGG
                     KTSLAKVIMGIYQQSQGKIYLNGEDISELSITERAHRGIAYAFQAPPRFKGLTIKDVL
                     TIAAPQAELLQQRKYLRDVGLCPEDYLDRDLGPGLSGGEIKRIEMAQLLLRDNSISIF
                     DEPEAGVDLWTIQKLITLLISSYRNNPNKTAIVITHNEKILPICDEIIVIEDGVVAGR
                     GSSSEIWHLIKDDIECKVRNQCQQGEMLYEIK"
     gene            complement(19073..19507)
                     /locus_tag="P5549_01255"
     CDS             complement(19073..19507)
                     /locus_tag="P5549_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRW11730.1"
                     /translation="MPYKSIDDRLAASNYRLTRQRKAVLDVMRENLSCHLTAEEVLFK
                     ARQKVPHIGIATVYRTLERLASMQVLYKTLLDGGKYRYEISVQEDHQHHHIICVACNR
                     IFEVEETLLDSLEKHLEEQGFAIVDHQLKIYAYCPDCKTQQV"
     gene            complement(19708..21039)
                     /gene="glnA"
                     /locus_tag="P5549_01260"
     CDS             complement(19708..21039)
                     /gene="glnA"
                     /locus_tag="P5549_01260"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRW11731.1"
                     /translation="MAQKNKAEILKQVKDCDVKFIRLQFTDIQGVMKNLAIPVSQLEK
                     ALDGEIMFDGSSITGFAEIDESDMYLIPDPDTFVIFPWRSSNGKVARLICDIYNPDGT
                     AFAGCPRNNLKRVLQEAEALGYTMNVGPEAEFFLFLADENGRATIQTQDEAGYFDLAP
                     LDLGENARRDMDITLEEMGFEIEASHHEVAPGQHEIDFKYGDALHIADAIMTFKLTVR
                     SIAQRHGLHASFMPKPIYGVNGSGMHTHQSLFKAGQNVFWDANSPDQLSDMARYYMGG
                     IMSHARALSAITNPTINSYKRLVPGFEAPVYIAWSGANRSCLIRVPAKRGLSTRIEVR
                     NPDPSCNPYLALAVMLRAGLDGIAKRMEAPAPVDKNIYKMDDAERKAVGIDSLPGNLL
                     EALQELDKDQIIIDALGEHIYTRFKEGKMQEWQDYNVQVTPWEVTNYLTKF"
     gene            complement(21052..21624)
                     /locus_tag="P5549_01265"
     CDS             complement(21052..21624)
                     /locus_tag="P5549_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HRW11732.1"
                     /translation="MSGVKIIIADSKENSRRILKDTLQHAGYQIQAEARNAPELLRKT
                     RRLFPDLVIIDANLEGGSPEEIAGIIEDDNLSSVLVITDDIHRRSLQEIAHLLKPYTE
                     ETLVSVIEVALLYRSKVASIQQEVTRLKENLSNRKLIEKAKGMIMKTQGLTESEAYRY
                     LQKQSMNRAMSMKDLAQEIILSEEKQSIIR"
     gene            complement(21803..22753)
                     /locus_tag="P5549_01270"
     CDS             complement(21803..22753)
                     /locus_tag="P5549_01270"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRW11733.1"
                     /translation="MKKSILAVVVLCLAMFLLGCNADKTDKPIAEVNGEKITQAAFNS
                     LYAVLKADYEIAQGTKLDESKDKKVIDQIKTNTYDNLVLQTLIRQDAAKQGIKLDSKE
                     VDEYLNYIKESKNATDKEGYNKFLEESKFTEKGLREYLETQQLNSKLAEKVTANIKAG
                     DKEASQYYEQNKSEFMDQGGIQIFHILVEDEKTAREIIDKLSGGGDFASLAKEFSIDT
                     SNKEQGGDLGLVNESTQFVPEFKTAALDLQPGEYTQEPVKSEYGYHIIKAGNKKEPQQ
                     KTFEEVKASIVAQLENAQKSQAYNDYMEKLKKNADIKDLR"
     gene            22970..24967
                     /locus_tag="P5549_01275"
     CDS             22970..24967
                     /locus_tag="P5549_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRW11734.1"
                     /translation="MKKMNLSTKLMLSGSIVILVICTVLGISSYIQAKKGLEAIVYET
                     ITDKAKDSARLIGNKLQLDLNSLYDIAQSSAVVSMDKNTQAGFLADVKRSMNYKELAV
                     ATPDGSIKFSDGHNYNIAKKEYFQMALKGKTNFSEPISNQLDANMQVYLASPIFLEGK
                     VIGVLVASYDGSNLYDISKNLNFSKTGYAYVINKYGIKIMHPRYDMVLAQDNDFINVK
                     KDPSLEELVELQKRMINGEIGYGEYTYKNNHKIMGFAPIPGTTWSLGVTAPMNELMSS
                     LDELKTGIIIFTLAILILGLLTTYSMGRRMSRSIIASIRQAKLMEAGDFTQDVDTAFL
                     GRNDELGDLSRALDRVTVSTRQLMSILSNSSHELAASSQQLLSAGEDIATNMKQSAFS
                     TAEIAAGMEEISAAMEEINAASQDMEYTLVKVREEANQVHVEAANIDKRALQVHKMSQ
                     QSQAATLAMYNEIQTKVLQAIEDAKVVKEISGLADNIAGIANQTNLLALNAAIEAARA
                     GVQGRGFAVVAEEVRKLAEDSAASVNSIQKLTKQVQNSIANLVNNANSLLNFINKDVV
                     RDYNLLVELGIQYKNDADMTKALTDTVSKNIENVIIAISEINKAIDATSATVQQTAAG
                     SQQIAKGSETTTELALSINAAARALSENAENLRNLISNIKV"
     gene            complement(25066..26400)
                     /gene="gdhA"
                     /locus_tag="P5549_01280"
     CDS             complement(25066..26400)
                     /gene="gdhA"
                     /locus_tag="P5549_01280"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRW11735.1"
                     /translation="MSNVNEILAQVIKRNPNEPEFHQAVKEVLESLDPVLEKHPEYTE
                     YAVVERIVEPERQIMFRIPWVDDNGKVQVNRGFRFEFNSAIGPYKGGLRFHPSVNASI
                     IKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDAEIMRFCQSFMTELYRHIGQDTD
                     VPAGDIGVGGREIGFMYGQYKRIKNIYEGVLTGKGLAWGGSLVRTEATGYGLVYFTEE
                     MLKEKNTDFQGKTVVVSGSGNVAIYAAEKIHQLGGKVVAMSDSSGYIVDADGIKLDTI
                     KQIKEVERKRIKEYVNAHPAAKYYDGCESIWTVPCDIALPCATQNELDEQAARTLVGH
                     GCIAVAEGANMPSTPEAVKVFHDNKVLFGPAKAANAGGVAVSALEMSQNSMRYSWSFE
                     EVDNKLKNIMIDIYQKSSEAARKYGFEGNLVAGANIAGFVKVADAMVAQGIV"
     gene            complement(26717..30184)
                     /locus_tag="P5549_01285"
     CDS             complement(26717..30184)
                     /locus_tag="P5549_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW11736.1"
                     /translation="MLQVNIDGKELKGYAGQTILELAQANGIAIPTLCHDERLKIYGA
                     CGLCVVEIEGSNKLARACATEINQGMVVITNSEKVRASRKMTLELLLSDHSGDCRPPC
                     VKACPAHTDCQGYVGLIANGEYLEALKLIREQIPLPASIGLVCPHPCEEACRRQLLEA
                     PVAIAALKTFAAEQDIYNDNPYIPVPKESTGKRVAIIGGGPAGLTAAYYLALEGHEVI
                     IYEAMPQAGGMLRYGIPQYRLPKEILDQEIRLIEALGVDILCNTRINRDISFDYIRQN
                     HDAVFIGIGAWKYTRIGCPGEDVEGVVGGIDFLREAALNGNVPIGQRVAVIGGGNTAM
                     DAARTAVRLGADEVMVLYRRTREEMPAEDIEIEEAEEEGVIFKFLLAPEEIMAEDGRA
                     VAIRCQKMQLGEPDSSGRRKPVPIEGEQEIIPVDTIVAAIGQQVDTSDLPGIQIDKWR
                     CIGTDTDSLQTNLQGVFAGGDAVTGPGIAIEAIAQGKKAALAMHAYLNGVELPVPGTF
                     TVERTGLTADDFAHVKRVERVKPVHRDPAVRRHDFLEIAGRMTPEQARQEGSRCLECG
                     CQDFFECKLIEYANEYHVDPRQLAGDKHPVMAYDEHPFIERDAAKCILCGLCIRICDE
                     HMGVGALGLVHRGFDSLVKPEFGRPLQDSECISCGQCISVCPTGALIEKNAHVKNVPL
                     AMDTVSSHCSFCGLSCGQVISSRGETVFRITPDQGETLCAKGRFGFAAGSERLRTPLL
                     KRGEELQECSWDEALTYIAKKSLAVKAKSEPGSIAIFASPSLTLEEAQIAASWQEMLA
                     TDQLSSFTPDAGRGLYKVLGENQATNSLDEMNGTDLILMLGSFNQNQVVPTRIRSAVR
                     QGAQLLVLSSEKTLLDDISEQCICPENHTAFLREIMAALAAQSLVDEKFVQTRTNGYR
                     ELLATLQKIVPGQQAQAIAAAYGKAKKAMIIVDGYTVSTEAVELMANLMVMAGKVGSA
                     RNGMIVINPGANSNGIWQAGFQQDGLQVTQALLEGKIKAAFILGEDPVEAGLVEAGDL
                     AKLEFLMAASPYLTATAALADVVLPMSTSLEVDGTYITADGRMVAIRAAKDSPAGQNN
                     LDIWAALSQRMNPNWDIDFVYPPERQSFLHTSGFAARDGKAVLAAGADGPIFVPVKIT
                     DPTLLKFRG"
     gene            complement(30201..31958)
                     /locus_tag="P5549_01290"
     CDS             complement(30201..31958)
                     /locus_tag="P5549_01290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRW11737.1"
                     /translation="MIIKIGIGSCGIAAGALPVWDVVQEEIGVQGQDIKLEQTGCIGM
                     CHHEPLLEMIYDDGRAYMFGHVTPEIAREIMQAEHPETLDKYLVSTSEQSNEFLSRQV
                     RIALRNCGHIDPESIDDYIKTGGYQALESVVKGNDPEGVIEQIKVSGLRGRGGAGFPT
                     WFKWNATRAEKNPQKYVVCNADEGDPGAFMDRSVLEGDPHTLLEGMAIAGFAIGASQG
                     IIYARAEYPLAVKRVEIAIQQARKKNILGKNIFGSQFSYDIIVKQGAGAFVCGEETAL
                     IASLEGERGMPRLKPPFPSQRGYWQKPTNINNVETLANVPWIIQEGGAAFAAMGTEDS
                     KGTKVFALAGKIKRGGLVEVPMGITLREIIYDLGGGIKDGRKFKAVQLGGPSGGCIPE
                     SLLDTPVDYKSISNTGAIMGSGGMVVMDEGTCMVDLARFFLNFTQKESCGKCVQCRIG
                     TKRMLEILNRICEGKGQEGDIELLENLGSQIIAGSLCGLGQSAPNPVLTTIRYFREEY
                     EAHIYDKKCPAHQCKELITYHIDAERCKSCGLCAKKCPVQCISGEKKQPYVIDQSKCI
                     KCGSCLEACPSKWSAVIVE"
     gene            complement(31955..32473)
                     /gene="nuoE"
                     /locus_tag="P5549_01295"
     CDS             complement(31955..32473)
                     /gene="nuoE"
                     /locus_tag="P5549_01295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRW11738.1"
                     /translation="MEKQFVQADVDLHLMDDILSMYSQEAGNLITVLQKAQDIYGYLP
                     LSVLQHIADGLGIKRAQVYGVATFYTQFRLTAMGKYIILLCEGTACHVNGSERIATAL
                     EEELKVSMGETTEDGLFTLSDAACLGCCSLAPVMMINGRAYGPLTPDKACAIIRDIYK
                     QEKMNAEGGVTA"
     gene            33218..33412
                     /locus_tag="P5549_01300"
     CDS             33218..33412
                     /locus_tag="P5549_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11739.1"
                     /translation="MLKYISVVVPEEIDRELRFACADQAVTKSRLVRKLIEEYLDDWR
                     QHFEGNIDMLDKELDDEDKK"
     gene            33835..34083
                     /locus_tag="P5549_01305"
     CDS             33835..34083
                     /locus_tag="P5549_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11740.1"
                     /translation="MTELQRIFLVYTGMYFVVIAALYLAFHVALIILEREARIKHAKC
                     KVLVARKRADEQERWKTAYALYNKKHQKEVPQRLETAV"
     gene            complement(34168..35178)
                     /locus_tag="P5549_01310"
     CDS             complement(34168..35178)
                     /locus_tag="P5549_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HRW11741.1"
                     /translation="MSEIAFLLLSLGIILVAAELFVNGVEWLGKIFNLSEGAVGSVLA
                     AVGTALPETIIPIIALLFNHGASGHEIGIGAILGAPLMLSTLAMFVTGMAVVIFRKKR
                     KSGVKVVGDYSTMSRDLGFFIIIFSVAVLAGMLPPEWRTYQLIIATIMVFSYIWYVHQ
                     MLTQERDIGDEEDIPSCYFAKDHDNPPLYRVIFQVVLALTMIIVGANLFVNSISEVAV
                     IYGVPAFVLAIIITPIATELPEKFNSVIWISRGKDTLALGNITGAMVFQSSLIPAIGI
                     FLTDWQLTRSALLSAVLAIAASVLLFVELRNRKHISAKMLLISGIFYVLFLIEVFIGL
                     IN"
     gene            complement(35201..35968)
                     /locus_tag="P5549_01315"
     CDS             complement(35201..35968)
                     /locus_tag="P5549_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRW11742.1"
                     /translation="MLVDYHVHALAHGEYEADQAWLNAYVDQACRRGIKEMGFSEHDE
                     FIAGLDRDTFRQVQNDRRQDLNLRLGIEIDYLPGGEEDILRKFSQADYDYWIGSVHFI
                     DGWAFDHPAYRQGFDEQDIDEIYARYAQILFNMVSSRLFDVVGHLDLVKIWGHRPRQK
                     TAVDHLRPVLQVIKENGLVVEINSAGLRKPVGEIYPSHDLLAALYSLNIPITLGSDAH
                     HPQQMGEGIQEAWAAARKAGYQSLVGFHQHRQIKIFG"
     gene            complement(36175..36933)
                     /locus_tag="P5549_01320"
     CDS             complement(36175..36933)
                     /locus_tag="P5549_01320"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRW11743.1"
                     /translation="MKLAGKVAVVTGGGTGIGQGAAVALAAEGAKVMVLGRRPEPLND
                     TVAQIQRAGGEALACPADLTDAVQIEKAVQVLKSRWGRADVLVNNAGSSMRKPFMKVT
                     MDELDELYRVDLRSVYAVTQALVPMLKEDGGGSIINISSILGVLGSAQSTAYCAMKGG
                     VVNLTRAMAAELGPDIRVNCLCPSHIVTPMMQGEMDRLEAAGKMDKLNRLFPMKRVGY
                     PEDMNGAILFFASPDSAWITGNYLMIDGGLSCYV"
     gene            complement(37039..38853)
                     /locus_tag="P5549_01325"
     CDS             complement(37039..38853)
                     /locus_tag="P5549_01325"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HRW11744.1"
                     /translation="MAEQIKVQIVPATGEASVYWALPGKTVQQILEMAGWESGGTCGG
                     QKTCGKCKVRIEGSTSPLEANEQQLLMPEEIRNGGRLACYCTLHGDATVYLDLMPAEQ
                     AVKNKITRYRPGQPRHSGVQYKSFFIPGRQAETSVPLLDRLQAALPDTLFELTPENLN
                     ELHKIDRPGRPALELHALLINRRVCHVEKAKKAILGLALDLGTTSLFAALLDLESGQV
                     LTTASQSNMQRIYGQDMISRLAYVQQHPDGAMSLHKIMINNINAMIDDMLSQTSLTGE
                     RIWKICAVGNPVMLHFFLGLETDGFYAAPFDGIFAASMEIPAALLELRVNPLARLVIP
                     PQLGGFVGADMTACLITMQNGLQFPFLLCDIGTNSEIAVCSPGGIWTSSAAAGPALEG
                     GALRCGMRAGIGAIERFGFDNERLTYQVIGNTTPKGICGSGAIDLLAILWENGCINRE
                     GTFTARAEELFSCRAGDQGQEIILPDQPDSGATPLVFTQEDIRQLQLAKGAIRTAIDI
                     LLHKASLAPADLTNIYLAGAFGSYLDAEKLIQIGLLPPVPRERIKNIGNAAAEGAMLT
                     LVEEDALFVAEQIKKQVHHVELAQEPNFQEDFLHNLNL"
     gene            complement(39188..39520)
                     /locus_tag="P5549_01330"
     CDS             complement(39188..39520)
                     /locus_tag="P5549_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11745.1"
                     /translation="MLAIFGSAAAEGGGSIFHNPLFVLLLVLVATYVFIKFCAWAKSF
                     QLSGKLKKWIFIFTGIGLLVFNVLYSKGNALVHESGDWSGATIALVGSLIWVFIFAFA
                     LMAETKAE"
     gene            39764..40101
                     /gene="rnpB"
                     /locus_tag="P5549_01335"
     ncRNA           39764..40101
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5549_01335"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(40150..40653)
                     /gene="mobB"
                     /locus_tag="P5549_01340"
     CDS             complement(40150..40653)
                     /gene="mobB"
                     /locus_tag="P5549_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRW11746.1"
                     /translation="MTAVISFIGRHNSGKTTVLSKVIAYLTQAGYKVAIIKHAHHHLN
                     IDVHKDSEMFLQAGAEYVCASSPGLILKYQHRESEADVYSLLREVPAEFDLVIVEGYK
                     NEALPKIEVLRGEIDRQPMLLPQTLALVSDFPLAAKVRVFSSQAAEEIARFILDALKL
                     EKPGTTT"
     gene            complement(40682..41977)
                     /locus_tag="P5549_01345"
     CDS             complement(40682..41977)
                     /locus_tag="P5549_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRW11747.1"
                     /translation="MSILIENASLVTMTEGNRVMEKAYLIIEDDKITALGCGQPPAGN
                     YTKRIDASDHLVLPGFVNTHTHAAMTLLRGYADDLPLKEWLETKIWPLEDKLSAEDVY
                     WGSMLAIVEMIKSGTTCFADMYFFVDQTARAVEESGLRAVLARGMVGVGPQAEKALEE
                     SQALVKEWSGRGQGRISFMLGPHAPYTCPPEYLQRVMRLAEELQVGLHIHIAETRGEC
                     ENIKQAYGRTPVAHLEHLGLFNLPVLGAHCVHLTEEEIDILARHNVGVAHNPESNMKL
                     ASGIAPVPPMLQAGIAVGLGTDGASSNNNLDMLQEMRSCALLHKVNHFDPTVLPAYQA
                     LEMATVNGARALRLNCVGTLAEGQKADIILVSLKEAHMIPRYDLIANLVYAAQAADVQ
                     TVIVNGKIIMENREIKTFDEAEVLRQAQQTARRLVGREG"
     gene            complement(42035..42685)
                     /locus_tag="P5549_01350"
     CDS             complement(42035..42685)
                     /locus_tag="P5549_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRW11748.1"
                     /translation="MQYLKERKQVLDVAQKIYREKMVPGTWGNVSLRVKGQPLLLITP
                     SGMAYESMTIDDIVLLDLEGEVVEGGRVPSIEKHLHAQVYKKRPDVGAVIHVHTIHAS
                     AFAVAGKSIPVILEETAQVIGHAVQVAAYAPCGTDRLAAETVDQLGAGNAVLLANHGL
                     VGVGQDLPQALRVCQIAEKTAQVALLAASLGQVNELCAEDINILNKQFKDYGQNKK"
     gene            complement(42714..43730)
                     /gene="mtnA"
                     /locus_tag="P5549_01355"
     CDS             complement(42714..43730)
                     /gene="mtnA"
                     /locus_tag="P5549_01355"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRW11749.1"
                     /translation="MTTIFWDQDAVVILDQNELPAQIKYVRCEDYRALVQAIKTLQVR
                     GAPLIGVTAAYGLALAVWKTQAGQAALDTLYDRAEQEFAASRPTAVNLFWAIRRMRNV
                     FKQCQGLNQSKTARILLDEADRILAEDVSINQRIGENGAALFTEKVRVMTICNAGALA
                     TGGYGTALGVIRSLHQNDNLQQVWACETRPVLQGARLTVWELSQDQIPVTLITDNMAA
                     YVMSRGEVDAVIVGADRIAVNGDTANKIGTYGLAVLAAYHKIPFYVAAPMSTVDLGMS
                     SGQGIPIEQRNHDEVRQIRGIYVTVPEVSVFNPAFDVTPGQLVTALITETGIIRSPYF
                     RSDR"
     gene            complement(43730..44521)
                     /gene="mtnP"
                     /locus_tag="P5549_01360"
     CDS             complement(43730..44521)
                     /gene="mtnP"
                     /locus_tag="P5549_01360"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRW11750.1"
                     /translation="MPEVAVIGGTGVYDPAILDNIRQINEKTRFGEVKVLVGTYKGYE
                     MAFIPRHGADHSVAPHLINYRANIMALKQLGVNTIMATAAVGSLHEAFKPGEYVLADQ
                     FLDFTKVRQHTFYEGGADGVVHCDMTIPYCPEVRAALEKSGQAQGLTVHNGGCYVCTE
                     GPRFETAAEIKMFQQVGGQVIGMTSVPEVCLAREMGICYGNISIVTNYAAGISSAILT
                     HAEVLEMMGRSIGKVRSLIMESVQYLKQNRQCDCRNILTETSLKQ"
     gene            complement(44568..45479)
                     /gene="ftsY"
                     /locus_tag="P5549_01365"
     CDS             complement(44568..45479)
                     /gene="ftsY"
                     /locus_tag="P5549_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRW11751.1"
                     /translation="MFERFKLGLSKTRQNIGGKIDSLIKRTRKLDEDFFEELEEILIQ
                     ADVGMDTSLALVEKVRSAVKKQKINDPLQVTELLQAAIAEVMGTEQVPLHIGTDKPAI
                     ILVVGVNGAGKTTTIAKLAYKYKQEKKKVVLAAGDTFRAAAIDQLQIWADRTGVELIK
                     HQEGADPGAVVFDAISAARARKADLLIIDTAGRLQNKTNLMKEISKIRKVIEKEMPTA
                     PHEVLLVLDATTGQNAISQARIFSEATGVSGIVLTKLDGTAKGGIVIAVARELGIPVK
                     LVGIGEGMEDLREFSPEIFARALFDSE"
     gene            complement(45776..49360)
                     /gene="smc"
                     /locus_tag="P5549_01370"
     CDS             complement(45776..49360)
                     /gene="smc"
                     /locus_tag="P5549_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRW11752.1"
                     /translation="MYLKKLEIKGFKSFAENTELLLEPGINVIVGPNGCGKSNIADAI
                     RWVLGESNVRHLRGQKNEDIIFNGTDKQKAQSMAMVDMTIDNACGDLPVDYREVTIGR
                     RLFRNGESEYWLNKSRVRMKDIVGLFTGTGLGKRGYSLIGQGELEQVLNGQPFDRRLI
                     LEEASGTIKYRQQRDEVTQRLAASALDLQRVTDIMGEMEIHAAELSKKAARAQQYMTL
                     QEEFVKLDRALIAAEITDSHKNLSVRIEDLQQKKASGLMIQQNLDDLAVRLTDAEQEA
                     DACRQKLEEMKDKRYQLEAALGQSEAEIKLIGERIKNLQERLCTAGADTKKYARLLES
                     LNQDLEKHQDNMQSEQQQWQQRHQAFAQLQMEIKMLEEELKAKEELFAQKRLLIFAEA
                     TQESQLKNDLLSGEEKLKQSREKIERLQLRWEDSQDKIRQAEQKLQALLQHKQNSSVL
                     LQERQKQLDRILQAYQTSIDELENLEMQEQTLRRTVSDLENKQMGLKELESKLYGYSD
                     GVKAVFEAKARGDKKFQGIKDLIARIIDVPAGMETAIEAAVGKGLENIVTATMEDARE
                     AINLLKKKGLGRVTFLPLDGLRVQKLDDRQRQVWERKEGVLGLASRLVSYDSQYEKAI
                     EYLLGRVLVVSDLDRGMQVFRNSKVPLRIVSLDGEIINVSGAMTGGSSAGRNSTSLLK
                     RKAEARKLEQQLMEHTALLEQLRQNKQNVSAALKALDEEVKAGRNQIAEAEFQLELIS
                     KEQDSTAASLADEQEANLLHEQDIKIQQTYISAYNKEIAQTQQQLAQHSANSSKSARE
                     LEELKADLEEKRREYDILQERARSQQEQLEMKTQELEKVKKNMEQFMEVKNSYEISIN
                     EAEKLQIRLQQDIRRQEELMQNSGQKRLTQKHEIEALQRSYEEQQEIMHHHTKDIQAL
                     HDQCGPWQEQQNRLNEEIRLLEMRILRLEMEYESALEKWQEKYFGEDPSHFERPMGAA
                     ERKRVRQQVDVLKQEIELLGPVDLESINEYRELDARLEFLQQQSSDILEAREALEKLL
                     QDTEKIMARNFSQFLLLANESFKRTCQEIFGGGEANLVMEDDVADLGSGVDIEIKLPG
                     KRSQSLNLLSGGERALTCIAFIFSLLRLRPAPFCLLDEIDAALDETNLRRFTEFLQGM
                     AEGIQFIVITHRQPTIEAGSSLFGVTMPQEGVSAILSLNLEQSIDLAG"
     gene            complement(49436..50515)
                     /locus_tag="P5549_01375"
     CDS             complement(49436..50515)
                     /locus_tag="P5549_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW11753.1"
                     /translation="MRHYNIPIFIPHLGCPYDCIYCDQKKISAQNQAPDEQQVLEIVE
                     QHLATITHPAEIEAAFFGGSFTAIQTDQQEQFLRLLQPYIACNLIQGIRISTRPDCID
                     EQILARLQRYGVKTIELGVQSMHDEVLRQSGRIYQAEDVEKSCALIKAGGFQLGIQLM
                     VGLPDDNAQRDMETVRRTIALAPQMVRIYPTLVLAGTKLEQMWREGRYQALTLEEAIP
                     ICRDMLLQFAKAEIPVIRMGLYPGEELRKPGNIMAGPFHPAFGEQVEQAVFKEQAILA
                     IKFFQQQYGIDQRIVLGVNQRDLSKLLGKKRSNLLAIQRSLHLPEIKVMAEGGEKRDW
                     ISVSGEEAGSGQLVFDRMEFLHYLP"
     gene            complement(50553..51257)
                     /gene="rnc"
                     /locus_tag="P5549_01380"
     CDS             complement(50553..51257)
                     /gene="rnc"
                     /locus_tag="P5549_01380"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRW11754.1"
                     /translation="MKRKDTTHRFLEDNQISCRDEHYFSMALIHPSYAQENKTRHSNQ
                     RLEFLGDAVLNFVTADYLYTHYPNKAEGDLTKIRARVVCENALVDVAAQIDLGSYLLL
                     GRGEEKSGGRQRKSILADAVEAVIGAIYLDQGMPSARDFILKHLQETIHQTAEGEFRD
                     FKSRLQEIVQARGSHNVYYETLEESGPLHAKVFEVGVYYESHLLARGTGSTKKEAEQH
                     AAEKVLQDMHLLNQFL"
     gene            complement(51254..51499)
                     /locus_tag="P5549_01385"
     CDS             complement(51254..51499)
                     /locus_tag="P5549_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRW11755.1"
                     /translation="MQEFFEIARKILAEQLNINPDNITMETTFEDIEADSIDIVEMIM
                     ALEDMYDIEFPEDDLEDYPVVGELIKALYDYLVQNQK"
     gene            complement(51537..52493)
                     /gene="plsX"
                     /locus_tag="P5549_01390"
     CDS             complement(51537..52493)
                     /gene="plsX"
                     /locus_tag="P5549_01390"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRW11756.1"
                     /translation="MRIAIDAMGGDHAPVEIIAGAVEWNKKSDDQVLLVGQEEVIAAT
                     LQNFTYSTERIEIVPASQVITMDESPAVAMRRKKDASILVATRLVKQGEADAVLSCGS
                     TGAQMAAALFILGRFENIDRPAIVAAIPKRDGSHALLVDVGANVDCKPQQLLQFALLG
                     KTYAAIGLDINEPRIALLNNGEEEGKGNSLTVEAYSFLKAQTDLNFVGNIEGRDIFSA
                     KADVIVCDGFTGNILLKTIEGLAQEAVQSCLLEIEKIPSFLNKFDYSRVGGAPLLGVE
                     GVSIVCHGSSKREAVYNGIKTARDCVVKDVVNRQKLALNPSL"
BASE COUNT        12961 a        14101 c        11391 g        14140 t
ORIGIN      
        1 cagcacggcc accgatacag aagccaggga aacataccga ctgaaaaaga cgataaggac
       61 aaatatgatc gctaaaaaca aaaatacttt cggcatcagc aaaaggattg caccggcagt
      121 ggtggccatg cctttgccgc ctttaaaacc aataaacacc ggccagcagt gaccgattac
      181 agcggccagc acacaaactg ctgccagccc ttcaccacct gtctgcagac ctatccaggc
      241 cgccgccagc ccttttaaca aatctcccag caacgctgcc aatgccacac gtttgccggc
      301 cgttcgcagc gcattggtgg aaccaacgtt gccgctgcct actttgcgga tatcgattcc
      361 tttaaacaag cggatcatga tataggcaaa gggaactgat cccaacaggt aactgatgat
      421 aacaacaata atcttttcca accgtactcc tcccgtacct atgaaaatat attacgattc
      481 tttgttttcc cgtttacgca gaatcatttt gatcggggtt ccttcaaagc cgaaattctt
      541 gcgcaattca ttttccagat aacgcaaata ggaaaaatgt atgtgttctg gcttgttggc
      601 aaaaaacaca aatgtcggcg gagcgacccc gacctgggtc gagtacaata ttttaatctt
      661 gtgtcctccc ccgcccggca ggggatttaa caaaatggct tcattgatca cctgattcag
      721 ttcggcggtg gtgatacgcc gcacgtgctg cccagctaca aaatcagcca gttcgagcac
      781 cttgaagatt cgttttctgg tgaccgcaga gatatatagg atggggctgt aggccatgaa
      841 tttcagatcc tcccggatct ccttgtcgaa tttgttcatc gtatgctcat ctttttctac
      901 cagatcccac ttgttgacca ccaatatact ggctttgccc tgctcgtgca catagccggc
      961 aattctttga tcctgttcat tgactccttc gctggcatcc agcatgacga tgaccacatc
     1021 ggcccgctca atgcttttca gtgatctgat cacactgtat ttttcagtgg gttcttttat
     1081 acgtgatttt ttccgaatgc cggccgtatc aatcaggata tactggttgc ctccatattt
     1141 taacgaggta tcgatggaat cccgggtggt gccgggcact tcgctgacaa tcacccgttc
     1201 ctgtcccagc agggcattga caaaggacga tttacccacg ttgggacggc ccacgatagc
     1261 gatcttgatc gcgtcaggat cctctttata ggcggacaac ggagcaaaaa gagcaatcac
     1321 cgcatccaac aaatcattgg tattgcgtcc gtgcatggcg gaaacaggaa tcggttcccc
     1381 cagtcccaga ttgtaaaact caaagtactc cagctgttta tcaaaattct ccactttgtt
     1441 ggcggcgacc accaccggtt ttttgctgcg gcgcagaatc gcagctactt gttcgtcttc
     1501 atgggtgatg ccttccttgg tgtccaccac aaaaatgatc acatccgctt catcgatagc
     1561 caattcagcc tgcagtttga cttcccgggc gaacagttca ccggcatcaa aacgaatgcc
     1621 gccggtatca atgataataa attcgcggcc cagccactcc gaactgtcat agaggcgatc
     1681 ccgggtaaca cccggaatgt cttccacgat tgccttgcga ccaccgacca gccgattaaa
     1741 cagcgtcgat ttacccacat tcggtctgcc aacgatggct actattggtt ttgacatatt
     1801 gcatcctcca acgatcctgg taatttgcga ttcagtattt tttcgaccag atcacgggca
     1861 ctgccatcca caggaatgat ttgggcaccg cttttcttca ttatatcatc gacagtcatg
     1921 tcatcgagaa gaatatgctg gccatcccgc agcacaattt ccggcagcaa aatggttttc
     1981 cccgggaaac agctgcccaa tgctctgatg atgtcactgc cggtcaacaa accggtcaca
     2041 ttgacacccc cgccaaaata atggttttcc accgggagca gttcaatgcg caagccttta
     2101 atttgctgca agcggccaat gactgaatcc cataccggta tcgccgcctg accggtaagc
     2161 aaatagactt cccgcgcttt catttcttcc ggcagctgtc cttccaaatc agcaaattcc
     2221 tccagcaaca accgcgacaa gccgattccg ttctccaatt gacaatagtc atcataatat
     2281 tctgccgcag gcaacggctc tccagcccgc aaataaaact catcggccag atatacaaat
     2341 ccataaccca cttggctgcg aaaacgtttt tgccagccag cggccagttc gacaagcctg
     2401 cgggccagtg ccgtatccac cggttccatc accggcagtt tctcacgatg cccggtcagt
     2461 cctaccggca caattccaac cgactgtacc gaaggatgaa agcccgccaa ttcctcaatg
     2521 ctttcttcca acatcactcc atcgttgatt cccggacaaa gaacaatctg cgtatgtatc
     2581 tgaatgttgg cattcttaag tctctgcagg tcagctttta tattggcagc ccgctggttg
     2641 gccagcatcc gggcccgcac atcgcctcgc atgcaatgca ccgatatata gagcggactt
     2701 acatgcatgc tctcaatttt ctgccagtcg gcttccgtga gattggtcag ggtgataaaa
     2761 ttaccgtaat aaaaagaata gcgataatcg tcatctttta tatacaaggt acggcgcata
     2821 cggcgtggca gttgatcgac aaaacaaaaa acacaatggt tgtcgcagct tttcatttta
     2881 tcaaaaacga tgccttcgaa ttccaggcct agatcttcat catcctcttt ttcaatttcc
     2941 agttgccaga tttctccatt ggatttgcga atttccagca gcaaggtgtc ctcctggctg
     3001 tggaattgat aatccaacag atcataaatg ggatgatcat tgattgataa aaggaggtcg
     3061 ccggcctcca attccatttc tgcaccgatg ctgtccgttc ggacatgccg tatttcagct
     3121 gccacttcat ttatttcctc tctgtaaaca aaatccattc ttcattatgg aggaaaaaaa
     3181 gaacgatagc aagacaggaa atcactccat tgttttagac ctatacaacg aatcagattt
     3241 cgtcaaacct ggaatctgct gctgaatatt ttcatattcc agctgcagaa tacaagcgat
     3301 cagattggaa gacagtggtt tgagccaacc tatttgggcc aactggtgca gccaagcaat
     3361 caaccgttgg gatttgatca cgatggggat aatctccatt tcttcgggag aactgtctaa
     3421 aataatgcgc agttggcgaa gggttttgtt gaccatttgc acatcagaac cgacccccag
     3481 gcaatgaacc cgtcggttgc gctggtcgct tcctatatat agaggatgac cgccggactc
     3541 cagttggtaa tcgttaaaat acgggagtgt tttataatcc cggtccaacc cgggatcgag
     3601 atacaaacgg gctgccagca gtgcctgatg gacgccggtc gatcccaaaa aaagaatatt
     3661 cattgttcac ctctgtctgc aatcaaggac actgattttt acggtttcca ccaggttgca
     3721 catggcctca aatgcctgct gcgtgccctg aatcacaacc ggcctgccca acgcagggat
     3781 tccgatcctt cgggaagtaa atccccccag tttcatactg gcattcaccc ggtccatcac
     3841 attgaccacc aggagctggt tatccttgcc cagaatctca gcaacacccc tcagcaaaca
     3901 accgaaccgg ttgcccagac tttttttacc aagaatatat acctcattac catcctcatc
     3961 actgcccatg taatgaatgg agccaaaatc gccgttgctg gtcttgtcat agtagggcaa
     4021 ggccatcagt tcagccgccg tgggcagccg ttcccgggaa agcaaaccca aatgcagggc
     4081 tgccgcagtc actgaagaat gggatccgcc aaaacaatga taaataattt tcatcgtcta
     4141 tcctgtccga gagtattttt tcattaatgc ttaacaataa tatatacacc gttttccaga
     4201 tcatgaacga tcccacccag aatcctcgcc agtaacaaac aaatattttg ttgttcttcc
     4261 tgactggcag caataaaagt cggacatgca ccgctcactt tttccggccg tctacaaact
     4321 atcgccagaa tacagtggtt gagggacgtt tcgttcatca atctgccgcc tttctgcctg
     4381 ccagactgct cagcccgata ccttgggcag actccagcag cggcactttt ttcacgatat
     4441 caatcaggga ttccacgtct ggttcattgg gtacaatgaa aagcgccaaa taacccttgt
     4501 cgggattttt tctcgccatg ggatgcagtt ccgtttcatt gatttcaatt tttgaaccca
     4561 gaatggatac cacatcataa atgatcgcca tacgctggcc gatattgtcc agggtcatac
     4621 gagcgtcatc atcctgggga tgcaggatga tccccagccc ttccgccagg atcttttccc
     4681 gggtagcagg cagtcctaca ttcatgatca caacatcatc caccatcaac agcgatcctg
     4741 caaaatgcag cttgccccgg ctgatttcaa taacttcgcc cagttgcctt cccgccatcc
     4801 atttcaacga caacagaatg cagacaagac cggcgacgac gcccaatggc catgaccaga
     4861 tatgggtcac cgcactgctg atgagagcag tgctcataca aagataattt ctcgcttcaa
     4921 aaaccttggc aatcccttcg atataatcct gaccacgacc gatcaacttg ttttcatcca
     4981 gtttgcccag gctttcccgt tccatgttgc gaatgttgcg aaactcctgg gctgccagca
     5041 ccaaaaaagt gactgccaca taatcgggct tgctgatggc cggcaccacc atggcgccca
     5101 gcccggctgc aatgaaaccc atggccagat gcgtcagatg cccgtgggga taggtggggt
     5161 aatgacgata atcattgcgc agcatcacac acctgtccac aaaccccagg cacaaaccta
     5221 ccagaatgac aaccagataa gctttcatat tatcccttgg aggaactgcg gtttaagaaa
     5281 aagtatccgc caatcacgat cgccactacg gcaatcaaaa tgagcaaggt ggtggtcgat
     5341 ccctgatcac tctgcccggc gggcttttgg gcaggccctt ctggattggc cgaagcagtt
     5401 ttatcgctgc cattcatccc caataccact ggcaaggact gcgcaaaaag cttgacgcct
     5461 ttttccgctt cttctttgct gttggtatgc gcccctactt caatcaacat ggcccgcggg
     5521 ctgagatcct gattgtaatc tcccttgccg ataaagatac cttctgaaag ccctgggctt
     5581 accttgtcca tggccgcttt cagctgcttg gcgaattgca aattggtttg gctgttggga
     5641 ttggaacggc ccaccaccag tttgattttg gttacatcct gacctttgac attggcctgg
     5701 tattctccgg gaggagtcgc atcacgatga acatcaatga tggccgccgg catttctttt
     5761 aaaagcgtac tggcagtctt gcgggagcgg ttataggcgt tgatatcgtg cggattatga
     5821 ttgttcttgt tatagactac cttaaacccc tgtgcctgca gttgtttgac gaacgcttcc
     5881 cccacatcat aaataccacc gttgccgttg atgctttcct tgccgtcgct gggcacatag
     5941 gattcgtcgc tgtgggtatg ataaaccgca atcttgttat tggctgcctt gctgaccgca
     6001 ggtaaaatcc cgccttccaa caaggattgc agtcgggcag tgtccaggga aggcagtgtt
     6061 tcctgaccct gataaacaca acgtgccgta tagccagaaa cttcaacgac cttgaatttg
     6121 gaattgtttg ccgcaatata gacatctccg ggatagacct gagtgccggt ttgatggatt
     6181 acatgattgt cttcatcgat cagactaaaa taccggtttg acggcaattc ttgctgggca
     6241 accacaagcc cggctgttat gagcaagaaa agcagcatga cgctggccag tgcaaatagt
     6301 ttcttatgca tcttcatcac ctcgttccgg ggcggacttt gcctgcggtt tgcgagctgg
     6361 ttcaggctcc cgcaaatttc tgatcaattc ttccggtcgc ccttcagtcc tgggtccccc
     6421 ttgaatgcgt tcccggcttt ccccgatcag ttccgccagc aacacggcca gaatacctgc
     6481 cagaatcagg gaatcaaaag ctcctccacc gccgatgtgc accgtacctc tcacaccggt
     6541 actgatcaaa taaacaaatt gtcctacgtc cagggaaaaa acacctatca ccgccgcaaa
     6601 aaaggcactg cgacgggaac gtccggccag atatccaaca ataccggcaa tcagcggata
     6661 caagtaaatc ggatcgataa agatctgctc cggctccgcc cccagaaatc gtccagccag
     6721 ataaagggcc aatgcagtaa tggcagcagc cgcgagcgca cgaatttttt catatccggt
     6781 gccagctccc agcaacacat acaaggctag aatcaccgca atcgaaccgc ctaaattgat
     6841 tgtaatcctg gtacccagcg gaatatcaat aaaacttccc acaatgatgg cagcaataac
     6901 cgccagggca gtccgatcat taagcctcat tcggtccaga acccgatgag caacgccaaa
     6961 gtatatcaag atggaaacaa tgattaaggc aatcatacca gcagggatat tccacatata
     7021 ttattccttc ctttctaata actattttta atcatattgt ttccccctcg tctttttttt
     7081 atgaagcgat ctggcgaaat gcagaaaaag aaaaaatgag tcaatcataa tgactcattg
     7141 cagataaaca ctcttccata taatcatcac taatatgatt ggttatgttt tattcggagc
     7201 gctcgctcca ttgggccgga tcaattttgc gccgggcaag ccactgtgcc gtttgatcaa
     7261 agataagcaa tggtttttgc tgcatttccc ggcaagttgc tgccccgctc atcagaaaca
     7321 cggcttggag ctggtacagg aaccgttctg ttttccgttc cagttcagcc gctccttcct
     7381 gcaacaggat acgcagaaac aaacccgaaa taccaaccat atctgctccc aaagcgatcg
     7441 ctttggcagc ctcagaggcc gatctaatcc cgccggaagc aatgatttta atgggcagct
     7501 gtaaggcagc aatctctgcc agactggcgg tagtggctat accccagtga taaaatgacg
     7561 ggtcaaacat tcctccgcgc tgatcttcaa tggccatgaa gttggttccg cctttgcctc
     7621 cacaatcgaa gaaatggact ccccgggcat gtattttgac cacgctctcc cgggacaaac
     7681 caaaaccaac ttctttggca atcaccggca ccggacaacg ggacacaatt tcacccacat
     7741 tgtccagcaa ccctctaaaa tttctatctc cttcctgcat ggccagttcc tgtggcagat
     7801 taaagtgcag ttgcaatcca tccgccgcaa tcatctccac tgcctgcaag gccttttccg
     7861 ctggacagcc ggcgctgaca ttggcaatca gcactccgtc agggttggtt tcacgggcga
     7921 cgataaaact gtcggccagc tgtcggtctt ccatggccac cgtttgcgag cctaccgcca
     7981 tggccaaacc gtatctggcc gccaacgctg ccaattcacg gttgatcgtc ttggcctctc
     8041 cgctcccgcc ggtcatggca ttaatcatga tcgggcacgc cagtgttttg cccaggaagt
     8101 ggctgcgcaa atcaatgtca tccataccca actccggtac ggcatccggg ataagcacaa
     8161 tatcggcaaa tccattttgt tcaggcccgt ccaaaacttg caaggccagc tgtaaatgtt
     8221 cgttttttcg ctgctctctg attacagata cctcccagca atgtttctgg ccaaactcag
     8281 ttgatcccga tcatttacat tcatgaaaat ttcgcggata ttgccaaact ggcataattc
     8341 ctgctcagca atggttttgg cattcagttc ttcgaatacg cgaatcagct tcagccggtc
     8401 attttgcaaa caatccgtca gcaccggcag acacttcttg ctgtaaatcg ctgacaaggg
     8461 ctggagtttc ccatcgattt ggggaaccac tccatcataa tcccccaact gcgccagcat
     8521 atacgcaacc agctccatat tcataaaagg catgtcgcag cccataacaa ataccgcatc
     8581 aaaacaggca tggtataaag cagaatgaat cccggccacg ggtcccaatc ccggataaac
     8641 atcttcacaa acccgtgttc ccatttgcct gtaaaggttt ggttcgttgc tgatggtgag
     8701 aatatcctcg aaagattgct gaaacttctc cacaataatt tgatagacag acttgtccgc
     8761 aatcacagca aaagccttgt tgaatttcat tcgggtgctt ttgcctcccg ccagtattgc
     8821 tccgctgcaa tgcatcgctt aacgtagatc aggcgaaaaa ttaaaggtaa cattgaaaat
     8881 gcgttcaatg gaatgaatat cttccttcaa ttgatgcaaa ttcttgatgg gttcgccttt
     8941 gtgtttaact tcaatgctga tatgttccct ttggtgatag gttttggcaa tatcgttgag
     9001 aatccgcaga ttgatctgct ccacgacatt caccacatca tcatcatcca acatccaacc
     9061 tctgtccact gcccaatctc tcctttcatg gtttattgaa aatactgtct atgaaagaaa
     9121 aaggctgtca actgacagcc taaaaacgca tccctcaacc ccccgaaccg gtactggaac
     9181 agctctggca ggaattctga gcaggtgcgt tgccactcga attggtggca aacacggata
     9241 aaagctgctt tatgttgcga gaaccgcatt ccgggcattg taccttgtct ttgtccttat
     9301 tggccaccaa aagatcaaat tggctggcac aatcgttaca cgcaaaatca aagattggca
     9361 tctccggcat accctttccg tttactcctc cgcagcaggt tctccagcag attcctggtc
     9421 atcctgatgc tgcaggtatt cctgcacttc actctctaaa accggttcgg cggcttcctt
     9481 caaagacaat ccgatccgtt tttcatcctt gtctatgctc aagactttca ctttgatgtt
     9541 ttggtttacc gccaccacat cctgaggttt ttccacccgg tagttggcca gctgggaaat
     9601 atgcaccagt ccgtccacgc ccggctcgat ttcaacaaac gccccgaaag gagcaatcct
     9661 gactactttt ccgtcaacaa tgtcaccctc ggtgtatttt tccataacga tttcccaggg
     9721 gctcttcaga agctttttgc ggctcaggga aactctctgt ttttcccgat ccagggccag
     9781 aatgaagact tcaatctcgt ccccttcctg gaatactgct gaaggatgat cgatgcgtac
     9841 atggtctatt tccgatacat gcagcaagcc ttcaaatccg cccagatcaa tgaaagcacc
     9901 atagtccacc aagcgtttga tccggccttt gataacctga ccttcttcga tggcagtcca
     9961 gaactgctcc cgagcttttg atttctctgc ttctaccagc tgtttgcggg ataaaacaat
    10021 ttgggaaccc cgctttttat tgcgattgaa ttcaatgatt ttaaaggtat aggttttgcc
    10081 gatatattca tcaaggtttt ttacataacc atcatctaca tgtgatgcag gcaaaaaggc
    10141 taccacccct acatcaacca gcagaccgcc cttgacgctt gatataatcg cgccttcgac
    10201 caagccacct tcattaaaag tctcttccag tttgtccatg gctcttttgg aatcaacttt
    10261 ctttttggac agcaggatgg ttccgtcatc atcccatttc agcacgagta cttcaatctc
    10321 ttccccgaca gtggccagga gtttggctga actgacgtct ttgaccgaca tctcccgcaa
    10381 cggaataatt ccctcagatt tgcctccgac atcgaccatt acgtcatcat catgtacttc
    10441 gaccacgatg cctttaatta catcgccgcg gttgggactg gccaaatcgg ccatttctgc
    10501 ttccattttg gcaaagtttt cttcaccggc ttcattttcc tggggcacat tggtttccat
    10561 tgggctttcc tgcatttgct cctgcggagc gccgtcatca aactgcgtca tcctgtctaa
    10621 tacctcctta attatccaat ccggagtaga agctccagct gaaacaccta ctgtatcact
    10681 cgccttgaac caggaaggat caagatcctg cgcagattca atcacgtggg tgaccactcc
    10741 ggtgcttcga cactcctgcg ccagggtttt ggtgttggaa ctgtttctat ctccaattac
    10801 cagcatccgg tcaacttgcg cgctcaacct tcgggctgct tcctgtcgtt tccgggtcgc
    10861 agaacaaatg gtattaaaca cgcgtacttc ttgtgctttc ggtataagta attccaccac
    10921 ggccaaaaac tcttcttcgt tgcgggtcgt ttgggagaca atccctaatt tcttgcatcg
    10981 tttttggctt tgcacttcat caatgctggt aatgacttgg gctttatgat cacaccatcc
    11041 caaaatcccc tgcacttcgg gatgatgaat atcaccatat actatgatat cataaccttc
    11101 gtttaacaac agccgtgctg ctgcatgtac ttttttcacc agcggacagg tcgcatcttt
    11161 gatctgcagt cccttttcct ccagcctggc gattgtctgc ggacctactc catgggatct
    11221 gattataatc ccttgaccgg acaggtcttt aatatcttcg acggacgcaa ggcctttatg
    11281 gctgaaatat tcaacgacag ccttattatg caccaatggc cccagtgtaa accagcttcc
    11341 ttcctccagg gccgtttcct cgctgatttg aaaagctctt ctgacaccgg cacaaaaacc
    11401 ggcttcatca gccacaataa gctgcaaatc atacacctca taatatacac taataatttc
    11461 gtagcaaaga agctaattcc tgctttattg acgcaaaagg tcgtttattt tctcggatac
    11521 ttcgttgctg agtttgccca tggtttccga attaatcttt tgcccctgat aatcaagcat
    11581 ggaaatgggt tctccaattc gaatcatcag cggtcggagc caaccacagg gcaaggtttt
    11641 atccgtgccg atacaggcta ccggcagaat cggcgccccg gaacgcaggg ctatcatggc
    11701 tgctccagcc tgggtattct ccacctgacc aggctttttg cgggttcctt cgggaaagat
    11761 tcccaggact ttgccatctt ccatcaattg cagtgcatgc cggatggcat gacggtcagc
    11821 attgcctctt tttaccggga aagcataaac ccgggccaat aggttaccca cgattttttg
    11881 gttaaacaac tcagccttgg ccataaaatg caccgggcga tcaatagcta cggccaccaa
    11941 caaaggatcc ataaagctca aatgattggc ggccacaatg acggcaccgc tgccgggaag
    12001 gttggcgaat ccttcaattt tcaaacccat aatcaagaat ataaatttaa ggactgctct
    12061 ggcgctattg taaagcatct tgcttctccc cgataatggc cagcatacgg tccagaacca
    12121 cttcgatatc caagtgactg gtatcaatca cgaacgcatc gtccaggatt tttaatgccc
    12181 ccatggcccg ttcccgatcc atgcgatcac gttcctcaat ctgccggcgg acacttgttt
    12241 catcttggat aaaaccctgt ttttccaact ccagcaggcg tcggcgggtt ctctcttcaa
    12301 tggaggccgt aacataaaac ttgatatcag cctggggcag aacctgttcc ccgatgtctc
    12361 tcccatccat gaccacatca ttcatttggg ccatgtcctg ttgttttttt accatcacgt
    12421 ttcgtacccg gggctgggcg gcaaccacag aaacattggc gttaatctgc ggactgcgaa
    12481 tggcttcgct gacatccacg ccatcacaaa acacttgttg ctgctcagcc tctttaacaa
    12541 aacggatatc cgtacggcca gccagttcac ggagtttttc ctcatcctgc aaatcaatct
    12601 ttttaaccag tgccttccat gtcaaagctc tgtacatggc tccggtatca atataaatat
    12661 aacccaattt ttcggctatc aggcgcgctg ccgtactttt tccggcaccc gcaggtccgt
    12721 cgattgctat ttgcattggt ttacctgcct aaccattttc tcatatattt tagcataacg
    12781 gctaatgata taaaagcaag tggcggaaaa tacaatcacg acgaacgtaa ccgtcaagcc
    12841 cgactcgtat ggtgaaatga tacagctcat tatttaacgg ttgtaatgcg ctggtttttt
    12901 cgctaatcta tagagtaaga cgcagcaaaa aggagaaagt tgccatgtca ttgtcgatga
    12961 attttcagct tcattatatt aatcaagagc agcatctaat gttgcctcga accagttcca
    13021 ttatcctgac ccaaaacttg tatgacattt tgttccaata tgtcattacc ccggaaaaag
    13081 aagccctgct tgacagtttt atcaaacggt tggagagcca tataaagagc aaggccgaag
    13141 cccccttctc cctgccggtc agcgaactgg aatttctgga agaaggtctg gaggaattgc
    13201 ggctgctgaa ttgggcagaa ataccggtat ccgtttttcg catcaatctt gatgcctgtc
    13261 tggacaagga gagaaccgaa gatgaaatgg aaaacatcgc aaccctgctg gcacagctga
    13321 tgatcatcag acgttatccc ggatctgatc tggtttatgt ctatccgtcc aatctggtcc
    13381 gctattaacc aaaattcaaa gatagtgtaa acgaaggagg tacaaatatg gatttaaaag
    13441 gatcaagaac agaagagaat ttaatgaagg catttgccgg tgaatcacag gccagaaaca
    13501 aatacaactt ctgggccagc caggcaaaaa aagaaggtta tgaacagatt gccggatttt
    13561 ttgaagaaac ggcagataat gaaaaagaac atgccaaacg tttgtacaag ctggcaattg
    13621 gcgggtccat caaatctacc gtggaaaatc tggtggaagc agcgcaaggc gagaacgaag
    13681 aatacaccag tatgtatccg gaaatggaaa gaatcgcccg ggaagaaggc tttgatgaaa
    13741 tcgctgatgt cttcagagaa atcggtgaag tggaagaagc ccatgaaaaa cgttatctga
    13801 aactgttgga aaacattaaa aacggtactg ttttcaaaaa atcatctccg gtcaaatgga
    13861 aatgccgtaa ctgcggatat atccacgaag gcaccgagcc cccggaactc tgcccggctt
    13921 gcgcgcaccc gcgtgcttac tacgaagagc tggccgaaaa ttactaaacc ccctgccctt
    13981 gagtggcgac cacaagcgaa ccacagtctg catgcaaaaa cccgctttcc ttacggaaag
    14041 cgggctattt ttttatcgaa aggccaacag tgcttcgcgc agtattttgg cccccaggat
    14101 cgaagtgttg tctgcaatat caaaaggcgg cgagatttcc accaggtcaa agcccacaac
    14161 atgcaaggag gccagctcca atatcgcttg aagaagatca cgcgaggtca cccctcccgc
    14221 ttcgggagtg ccggtaccag gcgcaaaagc cgggtcaagc acatcgatat ccagggtcaa
    14281 ataaaccggc cggcggccaa ttttggcttt gacctcggca atgaccgcaa ggaattgatc
    14341 caaataaaga ttgctgcaac gttgtgccaa ttgcaattct tcccgggtcc cggagcggat
    14401 ccccaactga tatagattcc tgtctcccac caactgcaaa acatggttca tcacgctggc
    14461 atgggatagg ggttctccca tatatccttc gcgcagatcg gcatgtgcat ccatctggat
    14521 caccaccaaa tccgggtaca cttccttgta ggccttgatg atgggaaaac tcaccagatg
    14581 ttcgccgccg atggaaaaga tcttcttctg catggccagc aaggcccggg cggtatcctc
    14641 catccgctcc agactgcgct gtacattgcc gaacggaatt tgcacgtctc cggcatcata
    14701 aaaggcaatc tcctccagcg acaaatcctg ataaatactg tactcttcaa cttcttccga
    14761 gacttcccgg atgcgataag gggccagacg cgtcccggga cgaaaactgg tggtagaatc
    14821 catcggcaag ccaatgatca cccgatctgc ttcttccaga tgatcccggc tggccaggaa
    14881 tatcgccttg cgctccagcc atgctaaatt ttgcatcatc attttccctg ccctatgatt
    14941 tcctgaacaa aagccggcaa ttcaaaagcg gcctggtgta cgccagcatt gtaatattta
    15001 caaggctttt ccggagaaga ttggatccgg cgcgggtcat acttcttgga accaaccgta
    15061 aagctccaaa gaccgctcgg gtaagtaggg atattcgcca ggtaaagctc ggtaatggga
    15121 aacacctgac caataccccg atacaccatt tggattacat tctggttaaa gaacggtgat
    15181 tcgctttgcg ccaccatcat gccgtcttct ttcaaagccg cataacagct ggcatagaat
    15241 tccggagaaa acaactgaat agccggtccg accggttcgg tggaatcaat cagaatgata
    15301 tcaaactcat ccttgtgctc ctgaatatac tttatcccat cgtcaacgat cacttcagct
    15361 tcggggtcat caaaggaaca cgacaacgcc gggaaaaagt cccgggcagc ctgaataacc
    15421 tgtgcatcta tctctaccaa aacgcctttt tccacctggg gatgacgcaa gacttcccgc
    15481 agtgcaccgc cgtcgccgcc gccaatgacc agtaccttgc gggggcaagg atgggaatac
    15541 aaggcaatca tgctgatcat ctcgtgatat acgaattcat ccttgtcggt ggtcatgacc
    15601 attccgtcca gcaacagcat gcggccaaac tgttcggtca caaccacggc cagatcctga
    15661 aaaggggttt tttccactct caatgtttca ctggccttgc agctaaaacc taaatagggc
    15721 gtttgatatt ctgtgaccca taattccaat tgacttcctc ctcaattcaa taaactgctc
    15781 ttagtaccat agaggaacgg ctgcaaaagc acaaccgata ttcatcaccg tgtgctcgac
    15841 agcagctact ttgacttcat caagttccaa attacgcatg gcaaaagctt cttccaccat
    15901 tctgacgacc tgttcctctg cctctttttt gctgcatttg ccggagaatt ccataatgac
    15961 tccgaaactg ttttttttga tgccgactcc caccgccgct gagatcatgt cccccggttc
    16021 ggaactggta atggagccgt aagcaatggg caacagtgat cctggcggga tgaccagatc
    16081 gggttcaaat tcacaaccgg gggggagaat actgcttact ctcaataaat tggtattgcc
    16141 aacacgagca ttcaacaatg ctccgtcaaa agcagtcagc gctgttgtac cttctgaaga
    16201 acctgcggtt acaaagaatt ttttgggcaa cggcaacacg taaaatacac ctccaaattt
    16261 cgttatctca ataacagaat aattatatcc aatgccttct taaaaagaaa taaaataaaa
    16321 atttccagac aaattcgtct gaaatgagta ttggatctac cggacacaaa tatgcattta
    16381 tgcccgatag atttcgtttg ttctgatcag gaaggattat tttttaaata gtcgaggaaa
    16441 gcattgacgg atttacggcg cactttttgt ttgggataaa tgacgtaaaa aactcgccga
    16501 gcttcctttt ccactagatt caaatagcac agtccggtcc ggggatggtc agcacggatt
    16561 acccagcggg atacaatgga gatgcccatc ccggcttcca ccatggccaa aaccaattcc
    16621 gtgctgcccg cttccatata aacatcaagc tgatccttgt taaaactggc agcgttcatc
    16681 atttcttcca ctgctttgcg ggttccgctg cctttttctc tgaagatcca cctctcctgc
    16741 tgcaaatccg ccaggctgca tctggattgc cgggccaatc tatgattcgg cggaacgact
    16801 gctaccagtt catcttcccg ccagggaaag ccttcggctt tgcgctggtt gagccaaccg
    16861 cccaccaacc ctatatcgag ttcccgttcg ccaattttgc tgaagatttt ctcggtatca
    16921 gcgatttcca ggctgatgct gatatgaggg tgctcttcct tgaacccttt gatcaaagca
    16981 ggaagaatat attgacccgg gatggtgctg gctcccaaca ggagactgcc cttgcggtct
    17041 tcggcaatgt cctgcatggc ttgctccaat ttgtccatgc tgcggagcag atcacgggca
    17101 tgactgtaca gtgtttcccc ggcttcggtg gttttgagcc ggcgccctga tctctccagc
    17161 aggaggatcc cgtacaagtc ttccaaggcc tgaatttgtt tggtaacggc tggctgggac
    17221 aagccgagtt cttctgcggt gcgcgaaaga ttacgggttt caaccaccct gatataggtt
    17281 ttaaatagat tcacattcat ttttccaatc tcctgcccag attatctcaa gaatccttcc
    17341 aatatcgcat ccaccgcttc tttttcagtc aaacccaagg tcatcagttt gatcagctgc
    17401 tcgccggcaa ttttcccgat tgccgcctca tgcgtcaaca ctgcttcggc atcttcggcc
    17461 atcaattcag ggatcgactt gatgcgggcc ttgtccatca aaatggcatc acattccacg
    17521 tgccccacgc aactggcttt gcccaccaag gcggcacgaa aaacctgata agagttttcc
    17581 tgcgcaacag cacgggacaa gacctgagct actcccccca caccttcgat aatgatttct
    17641 atatctgatt ccgcctgctg attgccttgt gtcaacaaac gctccacgat cttcaggttg
    17701 cctttttcat gcacatgggc ctcggtgcgg cgaaaggtgt catcaacccc tttgatttgt
    17761 accatttcca attcaaccac agcatccttt tcaacaatga tcaccgtagt gggattgaga
    17821 acgtttttcc cttctccacc accttctcca tagtgttttt caatgtattt caacttgccg
    17881 ccccgcctga cgataatctc atgaattcca tcatggcgtg aacttccatg tccgtcatta
    17941 tggattccgc agccggcaat tatagtagca tccgcatctt cacccacgat aaaggtattg
    18001 taaaccttgt cctgaaatcc tgactgggtt agtatcaccg gcacatgaac ggtttcattg
    18061 cgggtaccgg gcttgatctc caccaatagc cctggcttgt cgcccacgat tgaactcagt
    18121 gagatgttgg gggaggactg gcggatgaca gcttgtccgt cccgtcttat attgactgcc
    18181 cccttgggta ttccatgcaa ttccgtaatg gaattgagca aatccaggtc caaattattt
    18241 aatttcatat agcatttctc cttgttggca ttggtttctt actttgcatt cgatatcatc
    18301 cttgatcaga tgccatattt cggaggaact gccccggcct gccacgaccc cgtcctcaat
    18361 aactataatt tcatcacaga tcggcaatat cttttcattg tgcgtgatca cgatggcggt
    18421 tttgttggga ttgttgcggt aactgctaat caacagagtg atcagcttct ggatggtcca
    18481 caaatcaacg cctgcctccg gctcatcaaa gatcgatatg ctgttgtccc gcagcagcaa
    18541 ctgagccatt tcgatgcgtt tgatttctcc tccgctaagt cccggaccca ggtcgcgatc
    18601 cagataatcc tcgggacaaa gacccacatc gcgcaaatac tttctctgtt gtaacaattc
    18661 ggcctgcgga gcagctatgg tcagtacgtc ttttatcgtc aagcccttga agcgcggcgg
    18721 cgcttgaaac gcataggcga tgccgcggtg agctctttcg gtgatggaaa gctcactaat
    18781 atcttctccg ttcagataga ttttgccctg gctttgctgg tagatgccca tgatcacttt
    18841 ggccagggac gttttgccgc ctccattggg accggtgatg gcatatacct tgcccttttc
    18901 gaaatcaagg ttgacccctc tcagaatttc tttgtcttcg ccgctgtcac aggcgatttg
    18961 catccatata ttctcaagtt tcaacataat gttccaacct ccctatcgta aaaaatttat
    19021 aacaaccgct taatcggtcc ttcaatgata taattgctgc cggagatacg ctttatactt
    19081 gttgcgtctt gcagtccgga caatatgcat aaattttcaa ttgatgatcg acaatggcaa
    19141 atccctgttc ttcgagatgc ttctccaaac tgtccagcaa ggtttcctct acttcaaaga
    19201 ttcggttgca ggctacgcag atgatatgat gatgttggtg gtcttcctgc accgatatct
    19261 cgtagcgata ttttcctccg tccaacaggg tcttgtacaa gacctgcatg gacgccaaac
    19321 gttccaatgt tcgataaacc gtcgcgatgc caatatgagg cactttttgc cgggctttaa
    19381 acagcacttc ctcggcggtc aggtggcagc tcaaattttc gcgcatgaca tccagtaccg
    19441 ctttgcgctg gcgggtaagt ctgtaatttg aggctgcaag ccggtcatcg atcgatttgt
    19501 agggcatgac tacctcctca aaaaaaactg ttataactat agtatatcat ttcaggcaat
    19561 gtccagaaat aatcgcacga aatctgctgt cgttgctcca cctgctcccg ggcactatgc
    19621 tcgtccagag acgataaaat aaaaacaggc acctgaatcg tgaggtgcct gtttttcatg
    19681 gctgctcgat ttttcgccgc tattctttta aaacttggtt agatagttcg tcacttccca
    19741 cggggtgacc tgtacattat agtcctgcca ttcctgcatt ttgccttctt tgaagcgagt
    19801 gtaaatatgc tctcccagcg catcaatgat aatttgatcc ttgtcgagct cttgcaaggc
    19861 ctccagcaga ttgcccggca ggctatcaat accaaccgct tttctttccg cgtcatccat
    19921 cttgtaaata tttttatcaa ccggcgctgg cgcttccatc cgcttggcaa tgccatccag
    19981 gcctgcccgc agcatgactg ccaaagccaa ataaggattg cagctggggt caggattcct
    20041 cacttcaatg cgggtactta atcccctttt ggccggcact cgaatcaggc agctgcgatt
    20101 ggcccctgac caggcgatat ataccggcgc ctcaaagcct ggcaccaatc ttttataaga
    20161 attgatggtg gggttggtga tggccgagag cgctctggca tggctcataa ttccccccat
    20221 ataataacgc gccatgtcac ttaactggtc agggctgttg gcatcccaga aaacattctg
    20281 gcctgcttta aaaagcgact gatgcgtatg catgcctgag ccgttgacgc cgtagatcgg
    20341 cttgggcata aaagacgcat gcagaccgtg acgctgggca atggagcgca cggtaagttt
    20401 aaatgtcata atcgcatcgg caatatgcag agcatcccca tatttgaaat cgatttcgtg
    20461 ctgaccagga gcaacttcat gatgagaagc ttcaatttca aaacccattt cttccagagt
    20521 aatatccatg tcacgacggg cattctctcc cagatccagc ggtgccaaat cgaagtaacc
    20581 ggcttcgtcc tgcgtctgga tggtagcccg accgttctcg tccgccagga aaaggaaaaa
    20641 ctctgcttcc ggtccaacat tcatggtgta gcccagcgct tccgcttcct gcagcactcg
    20701 tttcagattg ttgcggggac aaccggcaaa agcagttccg tcaggattat aaatatcgca
    20761 gatcaaacgt gcaactttgc cgttggagga tctccaaggg aatatcacaa acgtatccgg
    20821 atcaggtatt aaatacatat ccgactcatc tatctcagca aatcctgtta tggaagagcc
    20881 atcaaacata atctctccgt ccagtgcttt ttccaactgg ctgacaggaa tagccaggtt
    20941 tttcataaca ccttgaatat ccgtaaactg caggcgaata aacttgacat cacaatcttt
    21001 tacttgtttt aaaatttcag ccttgttttt ctgtgccata atcatctctc cttatcttat
    21061 aatcgattgt ttttcctcgc tcaggatgat ttcctgggcc agatccttca tcgacatggc
    21121 ccgattcatg ctttgctttt gcaggtaacg ataggcttcg ctttcagtca atccctgtgt
    21181 tttcatgatc attcctttgg ctttttcaat cagcttgcgg ttgctgagat tctcctttaa
    21241 cctggttacc tcttgctgta tggaagcgac tttgcttcga tacaacaaag ctacttcaat
    21301 cactgaaacc aaagtctctt ctgtataggg tttcagtaaa tgggcaattt cctggagact
    21361 cctgcggtga atatcatcgg ttattaccag caccgaactg agattgtcat cttcgatgat
    21421 acccgcaatt tcttccggac tgccgccttc cagattggca tcgatgatta ccaaatcggg
    21481 gaaaaggcgc ctggttttgc gcagcagttc aggggcgttg cgagcttcag cctgaatctg
    21541 ataaccggca tgctgcaacg tatcctttaa aattcgccgg ctattttcct tgctgtctgc
    21601 aatgattatc ttgactcccg acacaatccg tcctcacctg ccttcaaaaa aaaagtccgc
    21661 agcatagccg gttcaagaac tgactaaaca acggacatct tcgccccaaa atttctttat
    21721 ttctttaaca gtatagtaac tgtaatctgt tttggcaatc ctctgccggc cgatatactg
    21781 tatacctcaa acggactggc aattaccgta aatccttgat gtcggcattt ttcttgagct
    21841 tctccatata atcattgtaa gcctggcttt tctgtgcgtt ttccaactgg gcaacaatgc
    21901 ttgcctttac ttcctcaaac gttttttgct gcggctcctt cttgttaccc gccttgatga
    21961 tatggtaacc gtattcactt ttcaccggtt cctgtgtata ttctcccggc tgcaaatcca
    22021 gggcagccgt cttgaactcg ggtacaaact gggtgctttc attgaccagc cccagatctc
    22081 cgccttgttc cttattgctg gtgtcaatcg aaaattcctt ggccagcgat gcaaaatcgc
    22141 ctcctccgct aagcttgtca atgatttccc gggccgtctt ttcatcctcg accaggatat
    22201 gaaaaatttg aatcccgcct tgatccataa attctgattt attttgttca tagtactgac
    22261 tggcttcttt gtcaccggct ttgatgttgg ccgtaacttt ttccgccagt ttgctgttaa
    22321 gttgctgggt ctccagatat tcccgcagtc ccttttctgt aaatttgctt tcctccagga
    22381 atttattgta accttcttta tctgtggcat ttttactttc tttaatatag ttaaggtatt
    22441 cgtctacttc tttgctgtcc agtttgattc cctgctttgc agcatcctga cgaatgagtg
    22501 tctgtaaaac caaattatcg taagtattgg ttttaatctg gtcaattacc tttttgtcct
    22561 tgctttcatc cagctttgtg ccttgtgcaa tttcataatc ggccttgagc acggcataaa
    22621 gcgaattaaa cgctgcctgg gtaatctttt ctccgtttac ttcagcaatg ggtttgtcag
    22681 ttttatcagc attacagccc agcaaaaaca tcgccagaca caaaaccact accgctagga
    22741 tgctcttttt cactaccatt cctccctctt tgtcatcaac actgccatac attatagcac
    22801 agttaaacag ttagcccaac ttgctggaaa cagttccata agcaagatga tgttcacaat
    22861 agcagagaga tgattgtaga caattttact gcagatacat tgttactaca tagttttgca
    22921 tctttctaca tgttataatt ataaggaatt tatgcaaagg aatgacgaca tgaagaaaat
    22981 gaacctgagc accaaactga tgttatccgg cagtattgtt attctggtga tttgcacggt
    23041 attgggtatc agttcataca tacaagccaa aaagggcctc gaagcaatcg tatacgaaac
    23101 catcaccgac aaagccaagg acagcgcccg actgattggc aacaaactgc aactcgacct
    23161 gaattcatta tatgatattg cccagtcatc cgctgtcgta tccatggaca agaacacaca
    23221 ggctggcttc ctggcggatg taaaacgcag catgaattac aaagaactgg cggtggcaac
    23281 ccctgacggg tcgattaagt tttccgacgg tcataattac aacattgcca aaaaagaata
    23341 ctttcaaatg gccttgaaag gtaaaaccaa cttttccgag cccatatcaa accagttgga
    23401 cgcaaacatg caggtatatc tggccagtcc aatcttttta gagggcaaag taatcggggt
    23461 gctggtagcc agctatgacg gcagtaacct ctatgacata tccaaaaatt tgaatttttc
    23521 caaaaccggc tatgcttatg tcattaacaa atacggaatc aaaataatgc atcctcgtta
    23581 tgatatggtt ttggcccaag acaatgattt tattaatgta aaaaaagacc cctccctgga
    23641 ggaactggtt gaattacaga aacggatgat taacggggaa ataggctatg gggaatatac
    23701 ctataagaat aatcataaaa tcatggggtt tgcaccgatc cccgggacga catggtcact
    23761 gggagtgacc gcccccatga atgaactgat gagttcactg gatgaattaa aaaccggcat
    23821 tatcattttc accctggcca ttcttatttt gggtctgctg accacataca gtatgggtcg
    23881 tcgtatgtcc cgcagtatta tagcctcgat cagacaggcc aaattaatgg aagcaggaga
    23941 ttttacccag gatgttgata ccgcctttct cggccgcaac gatgaactgg gtgatctgtc
    24001 gcgggcactt gaccgggtca cggttagcac ccgccaacta atgagcattc taagcaacag
    24061 cagtcatgaa ttggcggcct ccagccagca gttgttgtca gcaggcgaag acattgccac
    24121 aaatatgaaa cagtccgcct tttccaccgc ggaaatcgct gcgggcatgg aggaaatctc
    24181 ggctgccatg gaagaaatca atgcagccag tcaggatatg gagtatactc tggtcaaagt
    24241 ccgggaagaa gccaaccagg ttcatgttga agctgccaat attgacaaac gtgccttgca
    24301 ggtccacaaa atgtcacagc agtcacaagc cgccaccctt gccatgtaca atgaaatcca
    24361 gaccaaagta ttacaggcca ttgaagatgc caaagtagtc aaggagattt ccggtctggc
    24421 ggataatatt gccggaattg ccaaccaaac caatctgctg gccttgaatg ctgccatcga
    24481 ggcggcccga gcaggtgtgc agggtcgtgg gtttgccgtg gtcgctgaag aagtgcgaaa
    24541 gctggcagaa gactccgctg cctcggtcaa cagcatccaa aaactcacca aacaggttca
    24601 aaactccatc gccaatctgg tcaacaatgc caattccctg ctcaatttta tcaacaaaga
    24661 tgttgtccgc gactataacc ttttggttga gttgggtatc cagtataaga atgatgctga
    24721 tatgaccaag gctttgactg atacggtcag taaaaatatt gaaaacgtca ttatcgccat
    24781 ttccgaaatc aacaaagcca ttgatgccac ctccgctacg gttcagcaaa cggctgccgg
    24841 ctcccagcaa atcgccaagg gaagtgaaac aaccactgag ttggcactga gcatcaacgc
    24901 tgctgcccgt gctttgtcag agaacgccga gaatctgcgc aatcttatca gcaatatcaa
    24961 agtttaaatt ttaatatact gatacatcct gcagatggtt taatatataa caagcccgca
    25021 ccaattggat gatgcgggct tgttgtttat gcggataata ccaaattata cgattccctg
    25081 ggcaaccatg gcatcagcaa ctttcacaaa accagcgata ttggcacctg cgaccagatt
    25141 accctcaaac ccgtatttgc gggctgcctc actggatttc tggtagatat caatcatgat
    25201 gttcttaagt ttgttgtcaa cttcttcaaa gctccaggaa taacgcatac tgttctgcga
    25261 catttccagc gcggagactg ccactcctcc agcattggct gccttagccg ggccgaagag
    25321 cactttgtta tcgtgaaata ctttcactgc ttcaggcgtg gagggcatat tggctccttc
    25381 ggccactgcg atacagccgt gaccaaccag ggttcgtgcc gcctgttcat ccaattcgtt
    25441 ctgggtggcg cagggcagcg cgatatcaca gggaaccgtc cagatgcttt cacaaccgtc
    25501 ataatacttg gcggcagggt gggcattcac atattcttta attcgttttc tttctacttc
    25561 tttaatctgt ttgatggtat cgagtttgat tccatctgca tcaacaatat aaccggagga
    25621 atcactcatc gccaccacct tgccgcccag ctggtgtatc ttttccgccg catagatggc
    25681 aacgttgccg gacccggaaa caactacggt tttgccttga aaatcggtgt ttttctcttt
    25741 cagcatttcc tcggtaaaat aaaccagacc gtatccagtg gcttcggttc tgaccagact
    25801 gcctccccaa gccagtcctt tccctgtcaa aacaccctcg taaatgttct tgatacgctt
    25861 atactgccca tacataaaac caatctcgcg tccaccaacc ccaatatcac cggctggaac
    25921 atcggtgtct tgtcctatat gccgatagag ttccgtcata aagctctggc aaaagcgcat
    25981 aatttcagca tctgattttc ctttggggtc aaaatcactg ccgcccttgc ctccgccaat
    26041 cggcaatccg gtcagagagt ttttaaagat ctgttcaaaa cccaggaatt tgataatgga
    26101 agcattgacc gaaggatgga atctcaaccc gcctttgtat ggtccgatgg cactattgaa
    26161 ttcaaatctg aaaccacgat tcacctgcac cttgccattg tcatccaccc acggaatccg
    26221 gaacatgatt tgtctttccg gttccacaat tctttccacc accgcgtatt ctgtgtattc
    26281 agggtgcttc tccaggacag ggtccagcga ttccagtact tcctttaccg cttgatgaaa
    26341 ttccggttca ttgggatttc ttttaattac ctgggccagg atctcgttca cgttcgacat
    26401 ggttttttcc tcctttggaa aattatttgc ttttgctggg caccaaattt acatttagga
    26461 ttatagtttt aatttatgca aacataaata ggttttgcgc aaatatattg tattcatgaa
    26521 atgcctgaaa tatggtattt tgtatgcatt ataccgggct taaacctttg tgaataccac
    26581 caccctgaga cgacaaaaaa cctatgcacc tgacgtactg gatacgcaga tgcataggct
    26641 tttgccatgc ggaataaaac tcttagcaaa gaatcatatc gttggttgaa tcaatcgttt
    26701 tcgttttggc aatgatttat cctcgaaact tgagcaaagt cggatccgtg atcttgaccg
    26761 gtacgaagat cggtccgtct gcccctgcag caagtacggc tttgccgtcc cgggccgcaa
    26821 atccactggt atggagaaag gattgtcgct ctggcggata gacaaaatcg atatcccaat
    26881 tggggttcat gcgctggctc aaagctgccc atatatccaa attgttctgc ccggccgggg
    26941 aatccttggc cgccctgatg gcaaccatcc tgccatcagc cgtgatataa gtgccgtcaa
    27001 cttccaggct ggtcgacatt ggcaaaacca cgtccgccaa ggcagcggtt gcggtgagat
    27061 aagggctggc cgccattagg aattccagtt tggccaaatc cccggcttct accaggcctg
    27121 cttcaaccgg atcctccccg agaataaagg cggctttgat tttgccttca agcaaagcct
    27181 gcgtcacttg caaaccgtct tgttggaaac cggcctgcca gatgccgttg ctgttggccc
    27241 caggattaat aacaatcatt ccattgcggg ccgagccaac cttgccggcc attaccatca
    27301 gattcgccat taactcaacc gcctcggttg agactgtata gccgtccaca ataatcatgg
    27361 cttttttagc tttgccgtag gcagcagcaa tcgcctgggc ttgttgcccc ggaacgatct
    27421 tttgcagcgt tgccagcagt tctctatagc cgttcgttct ggtctgaaca aacttctcat
    27481 ccaccagtga ttgggcagcc aatgcggcca tgatttctct caaaaaggcc gtatgatttt
    27541 caggacaaat gcattgttcg gaaatatcat ccagcaacgt tttttctgaa ctgagcacaa
    27601 gcaattgtgc cccttgtctg actgcgctgc gaatccgggt tggtacaacc tggttctggt
    27661 taaaggagcc cagcatcagg atgagatcgg taccgttcat ttcatccagg ctgttggtcg
    27721 cctggttctc gcccaacacc ttgtaaagcc cgcgtccagc gtcaggcgta aaactgctta
    27781 gttggtcagt cgccagcatt tcctgccagg aagcagcaat ctgcgcttcc tccagggtta
    27841 acgacggaga agcaaagatg gcaatcgatc ctggttcgga tttggctttt acggccagac
    27901 ttttcttggc gatataggtc agggcttcat cccaactgca ctcctgcagt tcttctcccc
    27961 tctttaacag cggggtgcgt aatcgttcgc tcccggcagc aaatccaaag cggcctttgg
    28021 cgcaaagcgt ttcgccttga tccggggtga ttctgaacac agtttcaccc cgactgctga
    28081 tcacctgacc acaactcaag ccgcagaagg aacaatggct gctgacagta tccatcgcca
    28141 acggaacatt cttcacatgg gcattctttt caatcaatgc cccggtcgga catacactga
    28201 tgcactgtcc acagctgata cactcgctgt cctgcagcgg acgcccaaat tcaggtttta
    28261 ccagactgtc aaaaccccga tgcaccaagc caagtgcccc tacgcccata tgctcgtcac
    28321 agattctgat gcacagtcca cacaaaatac atttagccgc atccctctcg atgaaaggat
    28381 gttcgtcata agccatgacc ggatgcttgt caccggccag ttggcgcgga tcaacatggt
    28441 attcattggc atattcgatc agtttgcatt caaagaaatc ctgacagccg cattccagac
    28501 aacgactgcc ttcctgccgc gcttgttccg gtgtcatgcg tccggcaatc tccaggaaat
    28561 catggcgtct gaccgccgga tcacggtgaa ccggtttaac tcgctccact cttttcacgt
    28621 gggcaaagtc atctgccgtc aatccggttc tttcaacggt aaatgttccc gggacaggca
    28681 attccacgcc gtttaaatag gcatgcatgg ccaaggcggc tttttttcct tgggcgatgg
    28741 cttcaatggc gataccaggt ccggtaacgg catcaccgcc ggcaaatact ccctggagat
    28801 tggtctgcag gctgtcagta tccgtaccaa tgcatctcca tttgtcaatt tgtattcccg
    28861 gcagatcaga agtatccacc tgctgcccga tggcagccac gatggtatcc acgggaatta
    28921 tttcctgttc accttcaatc ggcaccggtt tgcgtctgcc ggaactgtcc ggctctccca
    28981 gttgcatctt ttgacaacgg atggcaacgg cccgtccgtc ttcggccata atttcttccg
    29041 gtgccagcag gaatttgaat attacgcctt cttcttctgc ttcttcgatt tcaatgtctt
    29101 ccgccggcat ttcctcccgg gtacgacgat agagcaccat gacttcatca gcacccaagc
    29161 ggacggctgt acgggctgca tccatggctg tatttccgcc gccgatgacg gctacccgct
    29221 gaccgattgg gacattgcca tttaaagcag cttcccgcag gaagtcaatt ccacctacca
    29281 ccccttctac atcttcgccg gggcagccaa tacgggtata tttccaggca cctatgccga
    29341 tgaaaacagc atcatggttc tgacggatgt aatcaaagct aatatcccga ttgattctgg
    29401 tgttgcataa aatatccacc cccaaggctt cgatcaatct gatctcctgg tccagaatct
    29461 ccttgggcag acgatactgg ggtatgccat aacgcagcat gccgccagcc tgaggcattg
    29521 cttcataaat gatcacttca tgcccttcca gagcaagata ataagccgca gtaagtccgg
    29581 ccggtccacc accgatgatg gccacccgct tgccggtact ctctttgggt acaggaatat
    29641 aaggattatc attataaata tcctgctcgg ctgcaaaggt tttgagtgca gcaatcgcta
    29701 ccggtgcttc cagcaactgc cggcggcagg cttcttcaca tggatgcggg cagaccaggc
    29761 caatgctggc cggcagtgga atttgctccc ggatcaattt taatgcttcc agatactcgc
    29821 cattggcgat caagccgaca tagccctggc aatcggtgtg agccgggcag gccttcacgc
    29881 aaggtggcct gcagtcgccg ctgtgatcag agagcagcag ttccagcgtc attttgcgtg
    29941 atgctctgac tttttccgaa ttggtgatga ccaccatgcc ctggttgatt tcagttgcac
    30001 atgcccgggc cagtttattg ctgccttcaa tctcaaccac gcacagacca caggcaccat
    30061 agatctttag tctttcatcg tggcacagag tagggattgc aatgccgttt gcctgtgcca
    30121 gttcaaggat ggtctggcca gcgtagcctt tcagttcttt gccgtcaata tttacctgta
    30181 acattgtctt cacccctcct ctattcaacg atgacggcgc tccatttgga aggacaggct
    30241 tccaaacagc tgccgcattt aatacacttg ctctggtcaa ttacataggg ctgtttcttt
    30301 tcgccgctga tacattgtac cgggcatttc ttggcgcaga gaccacagct cttgcagcgt
    30361 tccgcatcga tatggtaggt aatcagttcc ttgcattgat gcgccggaca ttttttatcg
    30421 taaatatggg cttcatattc ctcccggaaa tagcggatcg ttgtcaatac cggattgggt
    30481 gcactttggc ccagtccgca tagagaaccg gcaataattt ggctgcccaa attctccagc
    30541 agttcaatgt ctccctcctg gcctttgcct tcacagatgc gattgaggat ttccagcatg
    30601 cgttttgtgc cgatccggca ttgtacgcac ttgccgcagg actccttctg ggtaaaattc
    30661 aggaagaacc tggccaaatc taccatgcag gttccttcgt ccataactac cataccaccg
    30721 gaacccatga tggctcccgt attactgatg gatttataat caaccggggt atcgagcaaa
    30781 ctctccggga tacaaccacc ggaaggccct cccaactgaa ccgctttaaa tttacggcca
    30841 tctttaatac cgccgcccag atcatagatg atttcccgca gtgtgattcc catcggaact
    30901 tcgaccagtc cgccccgttt aatttttccg gccagggcga atactttggt gcctttgctg
    30961 tcttcggttc ccatggcggc aaaggctgca ccgccttcct ggatgatcca ggggacattg
    31021 gccagggtct caacattgtt gatattggta ggcttttgcc agtaaccccg ctgtgacgga
    31081 aagggaggtt tcagtcgagg cataccgcgt tccccttcca aagaagcaat caaggctgtt
    31141 tcctctccac aaacaaacgc tcctgccccc tgtttgacaa tgatgtcata ggaaaattgg
    31201 ctgccgaaaa tattttttcc cagaatattt tttttccggg cttgttggat ggcaatttcc
    31261 acccgtttaa cagccagcgg atactccgca cgtgcataaa taattccttg gcttgctcca
    31321 atcgcaaagc ctgcgattgc cattccttca agcaaagtat gcgggtcacc ctccagcacg
    31381 ctgcgatcca taaaagcacc ggggtcacct tcatccgcat tgcacacaac atatttttgc
    31441 ggattttttt ccgcccgggt agcattccat ttgaaccagg tcgggaaccc tgctccgcct
    31501 ctgccgcgca gtcccgaaac tttaatctgc tcgatgaccc cttccggatc gttgcctttc
    31561 accacgcttt ccagcgcctg gtatccgccg gttttgatat agtcatcgat tgattcggga
    31621 tcgatgtgac cacaattgcg caatgcaata cgaacttgcc gggacaaaaa ctcattgctt
    31681 tgctccgacg tggaaaccag atatttgtcc agcgtttcgg gatgttcggc ttgcataatc
    31741 tcccgggcaa tttccggggt gacatgtcca aacatgtagg cccgtccatc atcatagatc
    31801 atttccagca aaggctcatg atggcacatg ccaatacagc cagtttgttc aagttttata
    31861 tcctgaccct ggacgccaat ctcttcctga accacgtccc agaccggtaa agcaccggca
    31921 gcaattccgc agctgccaat ccctattttt attatcatgc tgtgacacct ccctcagcat
    31981 tcattttttc ctgcttgtag atatcacgaa tgatcgcaca ggctttgtct ggtgtcaacg
    32041 gtccatacgc acgcccattg atcatcatga ccggcgccag actgcagcaa cccagacagg
    32101 cggcatcgga caaagtaaac aaaccatctt cggtcgtctc gcccatggat acttttaatt
    32161 cctcttccaa cgctgttgca attcgttccg atccgtttac atgacaggca gtcccttcac
    32221 acagcaaaat aatgtatttg cccatagcgg taagccgaaa ttgagtataa aacgttgcta
    32281 ccccataaac ctgggcgcgt tttatcccaa gtccgtccgc aatgtgctgc aacactgaca
    32341 gcggcagata tccatagata tcctgcgctt tttgcagcac cgtgatgaga ttgccggctt
    32401 cctgactata catgcttagg atatcatcca ttaaatgcaa gtcaacatcg gcctgaacaa
    32461 attgtttctc cattctatct tctgcctgac aggagcaggt catggagaac acccctttcc
    32521 gttattaatt accgagacaa ttcatggttc tgacttgcct tggttacaaa caattgtcgt
    32581 tcgcaccagc cgttttactc cagtgcggga ataaataaag catcttacat tattataaca
    32641 atcatgattc aacacaaaat tctaaatttt ttccaattgc tcctaaacat aatcaattat
    32701 cgaacttaat gtacgaattg taaagacaaa cgtaggcaag ttcaatatta ttgtatattc
    32761 atacaaaacc aaaaccaact cttgttattc attatagtat agtctaaagc ttggttcttt
    32821 tcccgcatgg gtcagtgtct ggtttaataa cataatatga tattatgtta ttatgttatt
    32881 atatatttct ttattggaca tttttttagt atattagtgc gatttttgtg cttgttctgt
    32941 cattcacaaa tttcctctgt gataattgag aaaggagcat catgttatca tcttgttatc
    33001 tccctattct gtaaggttca cagcttatta aatgaatccg tttgtgtttg cgcttgtgat
    33061 aactagtaca ataaagattg cccacccctt gaacaagtcg aaatgccctt tttaaacagc
    33121 ataataacac caggcaattg taaatacccg tgtttttcga ataaaataat atcagaggaa
    33181 ttgtacgtca ataaaaaatc gaaagggggt aacatatatg cttaagtata tctctgtagt
    33241 ggttccggaa gaaatcgatc gtgaattacg gtttgcatgt gcggatcagg cagtaaccaa
    33301 atcacggttg gttagaaagt tgatcgaaga gtatctggat gattggcgtc agcattttga
    33361 aggcaatatt gatatgctag ataaagagct ggacgatgaa gacaaaaaat aatcaggttt
    33421 gaaaaaagtt ttatcaaaaa gtttgtttta atgataatcg tttgagcgga aataaaggaa
    33481 agccaatgaa aaataaattt cattggcttt cctttgtttt tggccatttt ttcaaattat
    33541 tctctccttt cagctgaatt ctctctcatt cttcaaatat cacattttgc tatttagatt
    33601 acatcatgtt atcttattat cataagatgt taataggttc acaggttaat tgggaggagg
    33661 ttatagctat ggaaggactc ttgttatcac tggcaattgc cttaaccttc ataagcggaa
    33721 cggcaggcac tctctatgtt tttaaaagag tatggctgga tgtttaagcg tatcagcagc
    33781 tgaacaaatt cagttattga atggataaat aaacactaga aagaggtgtt gaatatgaca
    33841 gaactgcaaa gaatattttt ggtttacacc gggatgtatt tcgtcgttat cgctgcattg
    33901 tatctcgcat ttcatgtagc gttgattata ttggagcggg aagcccgtat caaacatgcc
    33961 aaatgcaagg tactcgttgc caggaaaagg gctgatgaac aggaaagatg gaaaacagcc
    34021 tatgcactct ataataaaaa gcaccaaaaa gaagtcccgc agagattgga aacagccgta
    34081 taaccctcat acacacaaca ataaagccgg attctccaaa agaatccggc tttttgctgt
    34141 ctgtatgatt gttgaggttg catatattta gttgatgagc ccaataaaaa cttcaatcaa
    34201 gaacagcaca taaaaaattc cgctgatcag caacatcttg gcacttatat gttttctgtt
    34261 gcgcaactca acaaagagca gaacggaagc ggcgatagcc aagactgctg acaacagcgc
    34321 agagcgggtc agctgccaat cggtcagaaa tataccgatg gctggaatca atgaactttg
    34381 aaataccatg gcgcccgtta tgttccctaa tgccaaggtg tctttgcccc gcgagatcca
    34441 tatgacgctg ttgaattttt ccggcagctc cgtcgcgatc ggggtaataa tgatagccag
    34501 tacaaaagca ggcacgccat agatgacggc tacttcactt atggaattaa caaacaggtt
    34561 ggctccgaca ataatcatgg tcagcgccag cacaacctga aaaatcactc ggtaaagcgg
    34621 tggattatca tgatctttgg caaaatagca actgggaata tcctcttcgt caccaatatc
    34681 ccgttcctgg gtcagcattt ggtgaacata ccagatatac gaaaatacca ttatcgtggc
    34741 gataatcaac tgataggtac gccattccgg gggcaacatg cctgccagca cggcaacgct
    34801 aaaaatgata atgaaaaaac ccagatcacg gctcatggtt gaataatcgc caacgacctt
    34861 gaccccggac ttgcgctttt tcctaaatat gactaccgcc atgccggtaa cgaacatggc
    34921 caaggtggac agcatcaacg gcgccccgag gatcgcaccg atcccaatct catggccgct
    34981 tgcgccatgg ttaaagagca aggcaataat cggaatgatg gtttccggca aggccgtccc
    35041 gaccgcagcc aggacactgc ccacagcacc ttcggaaagg ttgaaaattt ttcccagcca
    35101 ttccacgcca ttgacgaaca attccgcagc gaccagaata attcccaaac tcaacaataa
    35161 aaatgcaatc tcgctcattg aacaaactcc ttcttgtcat ttacccgaaa attttgattt
    35221 gccggtgttg atgaaaccca accagggatt gataacctgc ttttctggca gctgcccagg
    35281 cctcctgaat tccttcgccc atctgctgcg gatggtgggc atcggagcct aacgtgatgg
    35341 gaatattcag ggaatatagc gctgccagca aatcatggga aggataaatc tcgcccaccg
    35401 gttttcgtaa accggcactg tttatctcca cgaccaggcc gttttcttta atcacctgca
    35461 gcactggtcg cagatggtca acagctgtct tttgccgcgg ccggtgtccc cagatcttga
    35521 ccagatcgag atgacccacc acgtcaaaca agcggctgga aaccatgttg aacaaaattt
    35581 gcgcataacg tgcatagatt tcatcaatat cctgttcgtc aaaaccctgc cggtaagcgg
    35641 gatggtcaaa agcccagcca tcaataaaat gtaccgagcc aatccaataa tcataatccg
    35701 cctgggaaaa ctttcggaga atatcttctt cgccgcctgg caggtaatca atttcgatcc
    35761 ccagacgaag attgagatct tgccggcgat cattttgcac ctgccgaaat gtatcccgat
    35821 ccaagccagc gataaactca tcatgctcac tgaaacccat ttccttgatg cccctccggc
    35881 aggcttggtc cacataagcg ttcagccagg cttgatctgc ctcatattcc ccatgcgcca
    35941 gggcatgaac atggtaatca accagcacgg tataaccctc ccggacttaa tttcattttt
    36001 acattgtacg cttatattcc atcaataatc aaggtatgtc aggacatttg cagtcaagtc
    36061 aaaatcccgg accataagca aaaccaaatc cgcgcctgct ttttgtcgga ttaaaccgtt
    36121 tcgcattgtc ggcacgggtc tggcttgttt ttctataatg gaaaagtacg tggcttatac
    36181 gtaacaggac aatcccccgt caatcatcag ataattgccg gtgatccagg cggagtcagg
    36241 tgaagcaaag aagaggatgg ccccattcat atcctccgga tatcccaccc gtttcatggg
    36301 aaaaagccgg tttagcttgt ccattttgcc agccgcttcc aggcgatcca tttcaccttg
    36361 catcatcggt gttacgatat gcgaggggca caggcagtta actctgatgt caggtcccaa
    36421 ttcggccgcc atggcccggg tcagattgac gactccgcct ttcatggcac aataagcggt
    36481 tgactgggcg ctgcccagaa caccgagaat agaagaaata ttaatgatgc tgccaccacc
    36541 gtcttctttc aacattggca ccagggcctg ggtcacagca taaacactgc gcaaatcgac
    36601 ccggtagagt tcatccaatt catccatggt aactttcata aagggtttgc gcatcgatga
    36661 accggcattg ttaaccaaga catctgctct tccccaacga cttttcaata cctgtacggc
    36721 cttttctatc tggaccgcat cggtcaaatc cgccggacat gccaaggctt ctccgccggc
    36781 tctctgtatt tgggcaacgg tatcattgag cggctcgggg cgacgaccca aaaccatgac
    36841 tttggctcct tctgctgcca atgcaaccgc cgctccctga ccaattcctg ttccaccacc
    36901 ggtaaccact gccaccttac ccgctagttt catttacttt acctcctgtt aaatgatatt
    36961 tggtctttta tcctttcggc ttatatgcga ttcatatgga ttgctccata acatagtaaa
    37021 tctctctttt ggaaacgatc aaaggtttag attatgcagg aaatcttcct gaaaatttgg
    37081 ttcttgggcc agttcaacat gatgaacctg ctttttaatc tgttcggcaa cgaacagagc
    37141 atcttcttcc accaaggtaa gcatcgctcc ttctgctgca gcattgccga tattttttat
    37201 tcgttccctt ggaacaggcg gaagcagtcc aatctgaatg agcttttctg catccagata
    37261 gctgccaaaa gccccggcca gataaatatt ggtaagatcc gccggagcca gtgatgcttt
    37321 gtgcaataaa atatcaatgg ctgtccggat ggcgcctttg gccaattgca attgacgaat
    37381 atcttcctgg gtaaagacca ggggcgttgc cccgctatca ggctgatccg gcaatataat
    37441 ttcctggcct tggtcacctg cccggcagga aaaaagttct tctgcccggg cagtaaaggt
    37501 tccttcccga ttgatgcaac cattttccca caggattgcc agtaaatcga tggcacctga
    37561 tccgcagatt cccttgggcg ttgtgttgcc gattacctga tatgtcaagc gctcattgtc
    37621 aaagccgaat cgctctatgg cgccaattcc ggcccgcatg ccgcatcgca aagcccctcc
    37681 ctccagcgcc ggtcccgctg ccgcagaact cgtccagatc ccgccgggac tgcagactgc
    37741 aatctcacta ttggtaccaa tatcacacag cagaaaagga aattgcaaac cgttttgcat
    37801 ggttatcaga caggccgtca tgtcggcccc gacaaaacct cccaattgtg gggggatcac
    37861 cagtcgggcc aaggggttga cccgcagttc caataacgca gcaggtattt ccatacttgc
    37921 tgcaaaaatt ccatcgaaag gtgcggcata aaagccatcg gtctccagtc ccaggaaaaa
    37981 atgcagcatc accggattgc cgacggcgca gatcttccaa atccgttcgc ccgtcaaact
    38041 ggtctggctg agcatatcat caatcatggc gttgatatta ttgatcataa ttttatgtag
    38101 agacatagcc ccgtccggat gctgttggac ataagccagt cgggaaatca tatcctgacc
    38161 gtaaatcctt tgcatattgc tttgcgaagc tgtggtcagc acctgaccgc tttcgagatc
    38221 aagcagtgcc gcaaacaagc tggttgtccc caaatccaat gccagtccca atatagcttt
    38281 tttcgctttt tccacatggc aaacccgacg atttatcaag agtgcatgca gttccagggc
    38341 aggtcgtccc ggccggtcga ttttatgtag ttcgttcaga ttctccggag tcaattcgaa
    38401 tagggtatcc ggcaaggcag cctgtaaccg atccagcagc ggaacactcg tttccgcttg
    38461 tcgtccggga ataaaaaaag acttgtattg tacgccgctg tgcctgggct gtccgggccg
    38521 atacctggtg atcttgtttt taactgcttg ctctgccggc atcagatcga gataaaccgt
    38581 ggcatcgcca tgcagcgtgc aataacaggc caggcgtccg ccatttctta tttcttccgg
    38641 catgagcagt tgctgttcgt tggcttccag cgggctggtg ctgccttcga tgcgcacttt
    38701 gcattttccg caagtcttct gcccgccaca ggtcccgcca gattcccagc cggccatctc
    38761 cagaatctgc tggacggttt tgccgggcag tgcccaataa acagatgctt cacctgttgc
    38821 cggtacaatt tgcactttga tctgttctgc catatcctca cctgcatttg cttatgatgt
    38881 caaaaatctc gtccaacgtt taaaagtttc aggctaataa tagtatagcg taaaatgtta
    38941 ttcacaggag agcaacccgg ataattttaa aaatctttaa aaaagaggcc gccctgacgg
    39001 cggcctccgg ttgtagcctt ttctaaaaga atagatgatt gtaaatttta tcctgtttgt
    39061 aagtgtatta tgccgtgagt aataaatgtt ccttcatttt gttacaccca agaacgtccc
    39121 ggtgtaaaac aacgaaaagt ggacagtccc tttggaactg tccactcaaa tctccattgc
    39181 ttttctgtta ctcggctttg gtttctgcca ttaaagcaaa agcaaatata aaaacccaga
    39241 tcaatgatcc gaccaatgca atcgtcgctc cgctccagtc tccgctctcg tgtaccaatg
    39301 cattgccttt ggaatacaat acattgaaaa ccagcaaccc aatcccggta aagataaaga
    39361 tccatttctt caacttgcct gacagctgaa agctttttgc ccaagcacag aacttaatga
    39421 atacataggt tgcaaccaaa accaacagca atacgaacag cggattgtga aatatgcttc
    39481 cgccgccttc tgctgcagct gacccaaaaa tggctaacac cttttcaccc ccctcgtttc
    39541 taatcccctg tttctcattt tattccctca cccaaggcaa aacaagagca ttctgggcag
    39601 atggatccaa actcatctca tgctctttcc ttcttttatt gatatagttc tccaaaagca
    39661 agtattttcc tgcctgttat tatttttttt tcaaatatat atttgtcagc gctggtgcat
    39721 tttcccctgg aatgtacaca ccggtttgta tataatggat aggcaagtaa accagatagt
    39781 cgcaggcaca ttggtgcctg aggaaagtcg gagctccatc gggcagggtg ctggataacg
    39841 tccagtgggg gtgaccccaa ggaaagtgcc acagaaataa accgcctctt tttgaggtaa
    39901 gggtggaacg gtgaggcaag agctcaccag caaacaggtg actgtttggc taggtaaacc
    39961 ccacccggag caagaccaaa taggggaaca tggaggtggc ccatctcgtt cccgggtaag
    40021 gtcgctggag cgtccaggca actgggcgtc gagatagatg actatccaat acagaactcc
    40081 gcttacagat ttgcttgctg agcaacggaa caactcacca tcagggggtt gttccgttat
    40141 cattttttat cacgtggttg tacctggttt ttctagcttc aatgcatcca gaatgaaccg
    40201 ggcaatctct tccgcggcct ggctggagaa taccctgacc tttgccgcca gcggaaaatc
    40261 gctgaccaga gccaacgttt ggggcaacag catcggttga cgatcgatct ctccccttaa
    40321 aacctcaatc tttggcaggg cttcgttctt gtaaccttcc acgatcacca gatcaaactc
    40381 ggcgggaact tcgcgcagca gtgaatagac atcggcctcg ctctcccgat gctgatattt
    40441 cagaataagt ccgggagaac tcgcacagac atattcggct ccagcctgca aaaacatctc
    40501 cgagtccttg tggacatcaa tattcagatg atggtgggca tgtttaatga ttgccacctt
    40561 atagccggct tgggtgagat aggcaatcac tttagacaaa accgtggttt ttccagaatt
    40621 gtggcgacct ataaaactga tcactgccgt catgtcaaag cctccctgtc ctcgctgatg
    40681 tttacccttc ccgacccacc aggcgtcggg ctgtttgctg ggcctgccgg agcacctctg
    40741 cttcatcaaa tgttttgatt tcccgatttt ccattatgat ctttccgttg acaatgactg
    40801 tttgtacatc ggctgcctgg gcagcgtaga ccaggttggc aatcaggtca tagcggggga
    40861 tcatatgggc ttcttttaaa ctcaccaaaa tgatgtcggc tttttgtccc tccgccagcg
    40921 tgccgacaca attcagccgc agcgcccggg caccgttgac cgtagccatt tccagggctt
    40981 gataggcagg caggacagtg ggatcaaaat gattcacttt atgcaacaag gcacaactgc
    41041 gcatctcctg cagcatatcc agattgttgt tgctggaagc accgtcggtg cccagtccga
    41101 ccgcaatccc ggcctggagc atgggcggta cgggagcgat cccgctggcc agtttcatat
    41161 tgctctcggg attatgggca acacccacgt tgtgacgagc caggatgtct atttcttctt
    41221 cggtaaggtg aacgcaatga gcccccagga caggaagatt gaacagaccc aagtgttcca
    41281 aatgagcaac cggggtccgt ccgtaggctt gcttgatgtt ctcacattct cccctggtct
    41341 cggcaatatg aatatgcaat cccacctgga gttcttcagc cagtcgcatg acccgctgca
    41401 ggtactcggg cggacaggta taaggggcgt gcggccccag cataaacgaa atgcgtccct
    41461 ggccacgtcc tgaccactcc ttgaccagag cctggctttc ttccaaggct ttctcggctt
    41521 ggggtccgac gcctaccata ccacgcgcca gtacggcccg caggccgctt tcttcgactg
    41581 cccgggcagt ttgatccaca aagaaataca tatccgcaaa gcaagtcgtt cctgatttaa
    41641 tcatttccac gatggccagc attgatcccc agtaaacatc ctcggcagaa agcttgtctt
    41701 ccagcggcca tattttggtt tccagccatt ccttcaatgg caggtcgtcc gcatagccac
    41761 gcagcagggt catggcagca tgggtatggg tattgacaaa accgggcaga accaaatggt
    41821 cggaggcatc gattcgctta gtatagttgc cggccggggg ttgcccgcag cccagcgcag
    41881 tgattttatc atcttctatg atcaaatatg ctttctccat cactcgattg ccttcggtca
    41941 tggtaaccag actggcgttc tcaattaaaa tgctcacttg gttaatccct ccctgtttgc
    42001 tggttaagca aaaatgctcc ccacataagc ctcattactt tttattttgc ccgtagtctt
    42061 taaactgttt atttaatatg ttgatatcct ccgcacacaa ttcattaacc tgtcccaaac
    42121 tggccgccag cagggcaacc tgggcggttt tctcagctat ctgacaaaca cgcaaagctt
    42181 gcggcaaatc ctgcccaacc cctaccagtc cgtggttggc cagcaacacc gcatttcccg
    42241 ctcccagttg gtcaacggtc tcagccgcca accgatccgt accacaggga gcataggcag
    42301 ctacctgaac ggcatgtccg atgacctgcg cggtttcctc cagaatcacc ggaatgctct
    42361 tccccgcaac cgcaaaggcg ctggcatgga tggtgtggac atgtatcaca gccccaacat
    42421 cgggtctttt tttgtaaacc tgggcatgca aatgcttttc gatggaagga acccgtcctc
    42481 cttcaacgac ttctccctcc aaatcgagca agacaatatc atcgatggtc atgctctcat
    42541 atgccatgcc gctgggtgta atcaacaaca agggctggcc ctttactctc aggctgacat
    42601 tgccccaggt gccgggcacc attttttccc ggtaaatctt ttgtgcaaca tccaatactt
    42661 gttttctttc ttttaaatac tgcatatgct tccctctttt ttttaaaatc cttttatcta
    42721 tcggaccgga agtaaggcga ccgaatgatc cctgtttctg taatcagagc agtcaccagc
    42781 tgcccgggcg tcacatcaaa tgccggatta aatacagaga cttcaggtac agtaacatat
    42841 attccccgta tttgccgcac ctcatcgtga ttgcgttgtt cgatggggat gccttgcccc
    42901 gatgacatgc cgagatcaac ggtggacatc ggggctgcta cataaaaagg gattttatga
    42961 taggcggcca gcactgccaa gccgtatgtg ccgattttat tggccgtatc tccgttgacg
    43021 gcaatgcgat ccgcgccgac gatcaccgca tcaacttcgc cccggctcat cacataagcg
    43081 gccatgttgt cggtgatcaa ggtcaccgga atttgatcct ggctcagttc ccagacggtc
    43141 aggcgcgccc cttgcaacac agggcgcgtc tcgcaggccc aaacctgctg caagttatca
    43201 ttttgatgga gggagcgaat gacgcccagg gccgtgccat aacccccggt agccaatgct
    43261 ccggcgttgc aaatggtcat aacacgtacc ttctcagtga acagagccgc accgttctcg
    43321 ccaatgcgct ggttgatcga cacatcctcg gccagaattc gatcggcttc atctaataat
    43381 atccgggctg tttttgactg gtttagtccc tggcattgct taaaaacgtt gcgcatgcga
    43441 cggattgccc agaataaatt gactgcggta ggccgggacg cagcgaattc ctgttccgcc
    43501 cggtcataca atgtatcgag tgctgcctgc ccggcctgcg ttttccatac cgccaatgcc
    43561 agtccataag cagccgtgac cccaatcagc ggtgcgcctc gcacctgcaa ggtcttgatg
    43621 gcctgcacca gggcacgata atcttcgcag cggacatatt tgatctgtgc cggcaattcg
    43681 ttttgatcca ggataacgac tgcatcctgg tcccagaaaa tcgtggtgat cattgcttga
    43741 ggctcgtttc tgtcaatata ttgcgacagt cgcactgccg gttttgcttc aggtattgta
    43801 cgctttccat gatcagggag cgtactttgc cgatactcct gcccatcatc tccaatactt
    43861 ccgcgtgggt tagaatggca gaagaaatac ccgccgcata attggtgaca atggatatat
    43921 tgccatagca gatgcccatt tcccgggcca gacatacttc cggcacactg gtcataccga
    43981 tcacttggcc gcccacctgt tgaaacatct tgatttctgc tgcggtttca aaacgaggtc
    44041 cttccgtaca cacatagcaa ccgccgttgt gaacggtcag accctgcgct tgccccgatt
    44101 tttccaaggc agcccggact tcgggacaat aaggtatcgt catatcacaa tgcacgactc
    44161 cgtcagcccc gccttcataa aaggtatgtt ggcggacttt ggtaaaatcc aggaactgat
    44221 ccgccaatac atattcaccc ggtttgaatg cttcatggag tgagcccacc gccgctgttg
    44281 ccatgatggt gtttaccccc aactgcttca aagccataat attggcacgg taattgatta
    44341 aatgaggcgc cacactatga tccgctccat ggcggggaat aaatgccatt tcatatcctt
    44401 tgtaggtacc gaccaaaacc tttacttccc caaaacgagt cttttcgtta atctgccgga
    44461 tattatccaa tatggcagga tcgtagactc cggttcctcc aatgactgca acctctggca
    44521 taacttcaac ctcccttaag ctctttatat ccagctccaa atcagcttta ttcactgtca
    44581 aacaacgctc gggcaaatat ctccggagaa aattcacgca gatcttccat tccctcgccg
    44641 attcccacca gtttgaccgg aatccccagt tcgcgggcta ctgcgatgac gatcccgcct
    44701 ttggccgttc catccaattt ggtcaatacg attccggaaa cgcctgtcgc ctcactaaaa
    44761 atccgtgcct gactgatggc gttctggccg gtagttgcat ccagtaccag caatacttca
    44821 tgcggcgcgg tgggcatttc tttttcaatt actttgcgaa ttttgctgat ttccttcatt
    44881 aaattcgttt tattctgcag acgcccggca gtatcaatga tcagcaaatc agctttgcgg
    44941 gcgcgggcag cacttatggc atcgaatacc accgccccgg gatccgctcc ttcctgatgt
    45001 ttgatcagtt ccaccccggt gcggtcagcc caaatttgca gctggtcaat ggctgctgcc
    45061 cggaaagtat cgcctgctgc caaaaccact ttcttttttt cctgcttgta tttatatgcc
    45121 agtttggcaa tagtggtcgt tttcccggct ccgttcaccc ctacaaccag aataattgcc
    45181 ggcttgtccg taccaatatg caaaggtacc tgttccgttc ccataacttc agcaatggcc
    45241 gcctgcaaaa gctcagttac ctgcagcgga tcattgatct tttgtttttt taccgcgctg
    45301 cgaactttct ccaccaaggc cagagatgta tccattccca catctgcctg aatcaatatc
    45361 tcttccagtt cttcaaagaa gtcttcatcc agtttgcggg tcctcttaat cagactgtca
    45421 atttttccgc cgatgttttg tcgggtcttg ctcaagccca gcttgaatcg ctcgaatatg
    45481 ccttttttaa atgtttccgc gctggtctgt tcagcttccg tctgttgcac agctaccccc
    45541 tcttcgcggt cagtcccgga ttcttcaagt ggttccactg gatccgtctg cgctgaggtt
    45601 tgtgccgtct gttctgccaa ctcatcagat accggtgttt tttcttcctc tgctgacagg
    45661 gcctcgccgg ctcttgggtt ttccagatag gactcctctg tttggatgtt ttcacgtttt
    45721 ttgcttttaa atctgtcgaa caatgccatg gaacgatcca cctttattgt ttgttttaac
    45781 ccgccaaatc aatactctgt tccagattaa gcgaaagaat ggcggaaacc ccttcctggg
    45841 gcatggtcac gccgaacagg ctgctgcctg cttcgatggt tggctgccgg tgagtaatga
    45901 cgataaactg aatgccttcc gccatccctt gcaagaattc tgtaaaacgc ctgagattgg
    45961 tttcatccaa cgcagcatca atttcatcca acaaacagaa aggagccggc cgcagccgca
    46021 gcaaggaaaa gataaacgcg atgcaagtca aagccctttc tccgccggac aggagattca
    46081 gagattgact gcgcttgccg ggcaatttga tttcaatgtc cacgccggag cccaaatctg
    46141 caacgtcgtc ttccatgacc agattggctt caccgccgcc gaagatctcc tgacaggtgc
    46201 gtttaaagct ttcattggcc agcagcaaaa actggctgaa attcctggcc atgatttttt
    46261 cagtatcctg caaaagcttt tccagggctt cccgcgcttc aagtatatca gaactttgct
    46321 gctgcagaaa ctccagacga gcgtccaatt cccggtattc gttgatggat tccaaatcga
    46381 ccggccccaa cagttcaatt tcctgtttga gtacatcaac ttgctgtctg acgcgttttc
    46441 gctccgctgc acccatcggc cgctcaaaat gggaagggtc ctctccgaaa tatttttcct
    46501 gccatttttc cagagcgctt tcatattcca tttccagccg caggattcgc atttccagca
    46561 accgtatctc ctcgttaagg cggttttgct gttcctgcca gggtccgcac tggtcgtgca
    46621 gcgcttggat atctttggta tgatgatgca tgatttcctg ctgttcttca taagaacgtt
    46681 gcagggcttc gatttcatgt ttttgtgtaa gacgcttttg tccggaattt tgcataagct
    46741 cttcctgccg ccgaatgtcc tgctgcaaac gtatttgtaa tttttctgct tcgttgatgg
    46801 atatttcata ggaatttttc acttccatga actgttccat attctttttt actttttcca
    46861 attcctgcgt cttcatctcc agctgttcct gctgggagcg ggctctttcc tgaagaatat
    46921 catattcccg gcgtttttct tccagatcgg cttttagttc ctccaattcc cgtgcagatt
    46981 tgctgctatt ggcggaatgc tgcgccagtt gctgttgggt ttgtgcaatc tccttgttat
    47041 atgcactgat gtaggtttgc tgtattttga tatcctgctc atggagcagg tttgcctctt
    47101 gttcatcagc caggctggca gcagtgctgt cttgttcttt gctgattaat tccaactgaa
    47161 attctgcttc tgcgatttgg ttgcggccag cttttacttc ttcatccaat gctttcaagg
    47221 cggcgctgac gttttgttta ttctggcgaa gctgctccaa aagggcagta tgttccatca
    47281 gctgctgctc cagttttctg gcttcagctt tgcgtttcag cagactggtg ctgttgcgtc
    47341 cggcagaact tccgccggtc atcgctccac tgacattgat aatctctcca tccaggctga
    47401 cgatacgcag cggcaccttg ctgtttcgaa aaacctgcat gcctctgtcc agatcgctga
    47461 ccaccaaaac tctccccagc aaatactcaa tggctttctc gtactgagaa tcatagctca
    47521 ccaaacgaga agccagtccc aatacgcctt cttttcgttc ccatacttgc cgttgacgat
    47581 cgtccagctt ttgcacccgc agtccgtcca gcggcaaaaa tgtaacccgg cccagtcctt
    47641 tttttttcaa caggttaatg gcttcacgcg catcttccat ggtcgcggta acgatgttct
    47701 ccagtccttt gccgacagcc gcttcaatag ccgtttccat tccagccggc acatcaatga
    47761 tcctggcgat caaatcttta atcccctgga attttttatc gccccgcgct ttggcctcaa
    47821 aaacagcctt gacgccatct gaatacccat agagtttgct ttccagttcc ttcagcccca
    47881 tctgcttgtt ttccagatcg ctgaccgttc tgcggagggt ttgttcctgc atctccagat
    47941 tttccaattc atcgatggag gtttgatatg cttgcaggat cctgtccaat tgcttctggc
    48001 gttcctgcaa aagaacactg ctgttttgtt tatgttgcaa caatgcctgc agtttttgtt
    48061 ctgcctgtct tattttgtcc tggctgtctt cccagcgcag ctgcagacgc tctatttttt
    48121 cccggctttg tttgagcttt tcttccccac tgagaagatc atttttcaat tggctttcct
    48181 gggtggcctc tgcaaaaata agcaagcgct tttgggcaaa taattcttcc ttggctttga
    48241 gctcctcttc cagcatcttt atttccattt gcaattgcgc aaatgcctga tggcgctgct
    48301 gccattgctg ttgttcggat tgcatgttgt cttgatgctt ttccaaatcc tgattgagcg
    48361 actccaacaa ccgggcatat ttttttgtat ccgcgcccgc tgtgcacaaa cgttcctgta
    48421 aattctttat tctttcccca atcagcttga tctctgcttc actttgtccc aatgccgcct
    48481 caagctggta tcttttgtct ttcatttctt ccagcttctg gcggcaggcg tctgcttcct
    48541 gttcggcatc ggtcagacgg actgccagat catccagatt ttgttggatc atcagccctg
    48601 atgctttctt ttgttgtaaa tcttctattc taacagacag gtttttatgg ctgtctgtta
    48661 tctcggccgc aatcaaggcg cgatcaagct tcacaaattc ctcttgcaac gtcatgtatt
    48721 gttgcgcccg ggcagccttt ttggataatt cggctgcgtg aatctccatt tcccccataa
    48781 tgtccgtaac ccgctgcaga tcaagcgcac tggccgccaa ccgctgagta acttcgtctc
    48841 tttgctgtcg atacttgatg gtcccggacg cttcctccag gatcaaacgc cgatcgaagg
    48901 gttgtccatt taaaacctgt tccagttcac cctgaccaat cagcgaatac ccgcgtttgc
    48961 ccaaacctgt tcctgtaaaa aggccaacaa tatccttcat ccgaacgcgc gatttattca
    49021 gccaatattc actttcacca ttgcgaaaga gcctgcggcc aatggtaact tcacggtaat
    49081 ccaccggcaa atcgccgcag gcattgtcga tcgtcatgtc caccattgcc atgctctgtg
    49141 ctttttgttt gtcagtgccg ttaaagataa tgtcttcgtt cttttgtccg cgcagatgac
    49201 gcacattgct ctctcccagc acccagcgaa tggcatcagc aatattgctt ttaccgcagc
    49261 cattgggccc gacaatcaca ttgatgcccg gttccagcag caattcggta ttttccgcga
    49321 atgatttgaa tcctttgatt tccagctttt ttaaatacaa aacagatcct cctgtataag
    49381 aatcactgct taagttcgtt tttctcagga ataattcctg ccccggtcag ccggtttaag
    49441 gcaagtaatg caaaaactcc atacggtcaa acacaagttg gcccgaacca gcctcttcac
    49501 cgcttacact tatccaatct cgtttttcac caccctcggc catgacttta atctccggca
    49561 gatgcagtga acgttgaatg gccagcaagt tgctgcgctt tttgcccagc agtttggaga
    49621 gatcccgctg gttcacccca agcacaatcc tctgatcaat tccgtattgc tgctgaaaaa
    49681 actttattgc caggatcgct tgttccttga aaacggcctg ttccacctgt tcaccaaagg
    49741 caggatgaaa tggtcccgcc atgatgttgc ccggcttgcg cagttcttct ccgggataaa
    49801 gccccatccg aatcaccgga atctctgctt tggcaaactg cagcagcata tcacggcaga
    49861 ttggtatggc ctcctccagg gtaagggcct gatatctccc ttcccgccac atttgctcga
    49921 gtttggttcc tgccaacacc aaggtcggat agatcctgac catctgcggt gcaagtgcaa
    49981 tcgtgcgccg gacggtttcc atgtcacgtt gggcattgtc gtccggcagt cccaccatca
    50041 gctgaatgcc cagctgaaag ccgccggctt tgatcagcgc gcagcttttt tccacatctt
    50101 ccgcctgata aatcctgcct gattgacgta aaacctcatc atgcattgac tgcacgccca
    50161 attcaatggt cttgaccccg tatctttgca aacgtgccaa gatttgctcg tcaatgcagt
    50221 caggacgagt cgagatcctg attccctgaa tcagattaca ggcaatataa ggttggagca
    50281 agcgtaagaa ttgttcttgc tggtcagtct gaatggcggt aaagctgcct ccaaagaagg
    50341 ccgcctcaat ttcggcaggg tgggtgatgg tggccagatg ttgttccaca atctccagga
    50401 cttgctgctc atccggcgct tggttttggg cagatatttt tttctggtca cagtagatgc
    50461 agtcataggg gcatcccaaa tgaggaataa aaatgggaat gttgtagtgc cgcatggtat
    50521 gtaattccct ctcttttgat acgtgccagc tttcagagga attggttcag caaatgcata
    50581 tcctgcagca ctttttctgc agcatgctgt tcagcttcct ttttggtgct gccagtcccc
    50641 cgggcgagca gatggctttc ataataaacg cccacttcaa aaaccttggc atgaagcgga
    50701 ccactttcct ccagggtctc gtagtataca ttatggctgc cgcgggcttg aacgatctcc
    50761 tgcaaacgcg atttgaaatc cctgaattca ccttcagccg tctggtggat cgtttcctgc
    50821 aggtgtttga gtataaaatc ccgcgctgac ggcatgccct ggtcaagata aatagcccct
    50881 atgaccgcct cgactgcgtc cgccaggatt gatttgcgct ggcgcccgcc ggatttttcc
    50941 tctccacgcc ccaacagcag ataggaaccg agatcaattt gggcagcgac atcaaccaag
    51001 gcgttttcgc acaccacccg ggcacggatt ttggtcaaat ctccctcggc tttgttggga
    51061 taatgagtat acagataatc tgcggtcaca aaattcaata ctgcatcacc gagaaactcc
    51121 aatcgttgat tgctgtgacg ggttttgttt tcctgggcat aagaagggtg gattaaggcc
    51181 atggaaaagt agtgctcatc ccggcatgag atctgattgt cttctaaaaa acgatgcgtt
    51241 gtatccttcc gcttcatttt tgattttgta ccagataatc atataaggct ttgatcagtt
    51301 caccgacaac cggatagtct tccagatcgt cttccggaaa ctcaatatca tacatgtctt
    51361 ccaaagccat aatcatttcc acaatatcaa tggaatctgc ttcgatatct tcaaaagtag
    51421 tctccatggt aatgttatcg ggatttatgt ttaactgttc cgctaatatt tttctggcga
    51481 tttcaaaaaa ctcctgcacc ccaccagcct ccttatgatt tgttcatcat attatattac
    51541 agggatggat ttaatgccag cttctgtctg ttgaccacgt ctttcacaac acaatcacgt
    51601 gcggtcttaa tcccgttata gaccgcttcc cgtttggaac tgccgtggca gacgatgcta
    51661 accccttcca cgcccagcag gggagcccct cccactctgc tgtaatcaaa tttgttcaaa
    51721 aacgagggga tcttctcaat ctccagcaaa catgactgaa cggcttcctg ggccaggcct
    51781 tcaatggttt tcaacaggat gttgccggta aaaccatcac agacgatcac atccgctttg
    51841 gcgctgaaga tgtcccggcc ttcaatgtta ccaacaaagt tcaggtctgt ctgcgctttt
    51901 aaaaagctat aagcttccac cgtcaggctg ttgcctttgc cctcctcttc gccgttgttt
    51961 agcaaagcaa tgcgcggttc attgatatcc aaaccgatgg cggcataagt cttgcccaac
    52021 agggcgaatt gcaacagctg ctgcggtttg caatcgacat tggcgcccac atccaccagc
    52081 aatgcatgac taccatcccg tttgggtatg gcggctacaa tggcggggcg gtcgatgttt
    52141 tcaaatcttc ccagaataaa caaggctgcg gccatctgag cacccgtact gccgcatgac
    52201 aatacggcat cggcttcgcc ttgcttaacc aggcgggttg ctaccaggat ggacgcatcc
    52261 tttttcctgc gcatggcaac ggcaggcgat tcatccatgg taatgacctg tgaagccgga
    52321 acgatctcaa tcctttccgt cgaataggtg aagttttgca atgttgctgc gatgacctct
    52381 tcctgaccga ccagcaaaac ctgatcatca gattttttat tccattccac cgcaccggca
    52441 atgatttcaa ccggagcatg atcaccgccc attgcatcaa tggcaattct caaatcgcct
    52501 ttcccccttt gcatggcttt tctctggtat gcggctgcat aaaaaacaaa aagtaagata
    52561 cagcctggaa gactatcgct tactttttgg ttc
//
LOCUS       DAOTHZ010000005        52003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_651_length_52003_cov_7.309232, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000005 DAOTHZ010000000
VERSION     DAOTHZ010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 52003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52003
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_651_length_52003_cov_7.309232"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="P5549_01395"
     CDS             complement(<1..170)
                     /locus_tag="P5549_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW11757.1"
                     /translation="MNILVCDDDKEILDAIGIYLKNEGYTVFKATNGLEALPLLEENP
                     IHLVIMDIMMPK"
     gene            complement(486..2501)
                     /locus_tag="P5549_01400"
     CDS             complement(486..2501)
                     /locus_tag="P5549_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW11758.1"
                     /translation="MSVNPKSKEIATEFIDYLHPENKSNVPWKEVKKLWQNLHNDPAL
                     RTGLRPEIYDSWERSYQYKVDRNLREIVHVCSDAEFSRAKTNSEYLMEIAIPVMERLS
                     EFVKGTGFLVALNDANGINLLNYGDKETLEWSKRTNLIEGTVWTEEIAGTNSASICLS
                     LVKPISVYSYEHFCLYAITAASSFAPIVDNGKVIGCIGMVAPYQRVSYHTLGMAVAAA
                     DHIQSKMALNRISKYQQVITDSMSDGVMVVDLNGLITYMNNKCSKILGFGTANMVGAN
                     IHDLFGQNPENQFFINIVNQGRMVTDDLVILYNGNNQIRCHITCTPLHGSILGDTGSV
                     IILRESERINNIVGKWIGRGAQMTFDDVIGSNPKFRDIIKIARTTAQSNSNVLLMGES
                     GTGKDIIAQAMHNESPRQNNPFVAINCAALPRELIASELFGYEEWAFTGAKKGGNVGK
                     FELANQGTLFLDEIADMPLDLQSTLLRVLEEKSVMRLGGNQVIPVNVRIMAATNKNLE
                     DEIARNRFRKDLYYRLGVIKLNIPSLRDRRDDIAPLVSHFLKLICKRYGKELYRISPL
                     ALQTLVSYDWPGNIRELQNVLEGCVQLAPGPEIDNDFIVNYMGLEADHLPKALSDVLV
                     PDFTATEELQLLEELLRKNKFNKKKTAQELGISRQTLYRHLHKYGLV"
     gene            2885..4780
                     /locus_tag="P5549_01405"
     CDS             2885..4780
                     /locus_tag="P5549_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW11759.1"
                     /translation="MSQGFYHTASHLPDNVAQMFNADLYNGDNNGQFTWKEMQHRVEG
                     IAGGLLSMGLEKQQRVAMMSTNSPYWTQADVATICCGGVLVTIFPTLSLNEVKYIVND
                     SESRFFYAGSKQILEKVLSGWDEMPSLEKVILLDMDYRSSDSRIIGLGELIESGSQYM
                     KSHYQEYEQIWKSLDLSDWATILYTSGTTGAGKGVILTHNSLSERVDGTHKYFVQAGH
                     PLDERDLVLSFLPLSHIFDRMCSQWLAIYRGATVAYADSAATLMNDLQKYNPTWFSCV
                     PRLYEKIYAQFNQMMAASAAKKKMFDWAYRVGEEALTYRRDEYGRYNMRQDYNLEARL
                     PSGLRMKYKVADKLFAKVRGLFGSRFRFSFSASAGIDPKLLTFFYVMGIPVLEGYGST
                     ETCSATNYNPMYAAKPGSVGPEANGSNCRLAEDGELEVRGGLFIGYLNKPEEDAAAFT
                     EDRWFRTGDLATMDDDGYYRIVDRKKAIICLATGKNVAPYKLESKFTTSLVVEQIFVL
                     GDERNVISALIVPSFAYFIDFYEKQNITYDRSQLIYTEMQGAQVCIGAGRDFIEQPIL
                     KDMIAENVRQVNETLEGFEQIKQYTIINHNFTEENGEMTPTLKLKKKAILEHYSEMIE
                     AMYQREY"
     gene            4978..7653
                     /locus_tag="P5549_01410"
     CDS             4978..7653
                     /locus_tag="P5549_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRW11760.1"
                     /translation="MSNFRRDLLNPDLKDILETFQLDKDYVHGEGTTLTDSDGNQYLD
                     FIAQYGAVPFGYNPSFVWEALERVRKRALPSLVQPSLPGEALRLANLLAECSPGDLCY
                     CTFCQSGTEAVEAAIKLARAATGKEMIISTGNSFHGKTLGALSATGKDSYQTPFRAPA
                     PGFVRIPYNDIAALEQALNDYAGNVAAFIVEPVQGEGGIVVAAPGYLQTTQQLCNTHG
                     VVFIVDEVQTGLGRTGRLFACEHEQVEPDVLLLAKALGGGLLPLGVCLSSPRVWNEDF
                     AMLHSSTFANNGVTCAVGIAVLEKLLQDQQALVKEVQQKGDYLLAKTRELQEKYPQAV
                     KEVRGQGLMVGLEFHPLDNCGSFDLAYLADQGGFTALLSGFLLNVYGIRLAPFLNNSM
                     TLRLEPTLTITCEEIDRVIKALEVCCQIMSWRDYSKLYRYLLGDRSPVTHIIDYRSQT
                     RTIKSSPLKPGEAAAHKFAFIIHYPAPEDVILNNPSFASMSRDELYRFLAWQSMTKDP
                     GIVCHMPAIRSKSGTIVEGWMIGVPFGGREIMQLPHAQTVDMIARAVDMGRDLGAEIV
                     GLGALTSVVTRGGRSVTGRDVAITSGNSFTTLMGVEALFQGAAKMHIETIAARGGVVG
                     ATGSIGRACALMLSEELSQLTLFGNPDHPVSSNNRLQSLAREIFVYASRRMQQGEMNG
                     ISLWLAQIRQLLLNRAEEPALTYLACLDKEDFSMTLMDEVCTYLDIDRPISISLSIPN
                     DLPECELVIATSNSPEYLIFADDLQSGAVVCDVARPSDVAPCVYERDDVLILEGGLVQ
                     YPDQISFGPNLGYRDGVSLACLTETVLLALEGECRDFSIGNKLSLETVHFLRNIARQH
                     GFALAGLKMGNHEINDEAIEKIYHHSLQLKRAGNL"
     gene            7664..8095
                     /locus_tag="P5549_01415"
     CDS             7664..8095
                     /locus_tag="P5549_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HRW11761.1"
                     /translation="MEYQEIKITREGKWFFGEAEMFRRNILQILAKNINRDDNGCYFI
                     EMGSERNPLIVEDVPFLAVGIVEEDEQPVKLVFHDLQEMVLNHELRLYIKEDVPYISY
                     RWPADTRLSRGVYWKLSDYFDFREDEVYIVPPAARPSSIPQ"
     gene            complement(8160..9035)
                     /locus_tag="P5549_01420"
     CDS             complement(8160..9035)
                     /locus_tag="P5549_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW11762.1"
                     /translation="MRYEGTVYRPPSEANSLLIQATIGCPHNKCTFCTMYKESRFKIR
                     PVEEIKEDLRSARDYYGEYVESLFFPDGNTIIMKTAQLVEIFSYAKQLFPYLQRITVY
                     GSARFVNKKSLEELQQLKDAGLSRVHTGMESGDDVTLQKICKGTTSAEIIAAGLKLKQ
                     VGIDTSEYYLVGIGGKERWREHALESARVLSAFSPDFIRLRTFVPVPGTPLYDDYKQG
                     KFNLLSPHEALQEIGLLISHLHCEGSYVLSDHVSNYWNVSGRLPDDREIMLEAVKTAL
                     AVPENRFRPAEISRL"
     gene            9259..10047
                     /locus_tag="P5549_01425"
     CDS             9259..10047
                     /locus_tag="P5549_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRW11763.1"
                     /translation="MEVKVLIVDDDERERIVQRYVLENIKDVEIVGEAVNGLDALLLC
                     QEKKIDLVLLDIKMSEMDGFETANKLVQLKNPPLFAFITMNKDMAVEAYELGAIDYIV
                     KPIEQNRIEKTISRSKAQIAHHHYIDELVNHKLRERIEEVLNRYKNQEQYAKKLPVRE
                     KGKIVLLKPEDIVFCESQGKKVLISTVDEGYLSSYTLNELENRLDQSYFFRAHQAFIV
                     NLNYIKEIINFGEGSYLLNMENSQKNIILSRSRAKNLREKLGIG"
     gene            10525..11736
                     /locus_tag="P5549_01430"
     CDS             10525..11736
                     /locus_tag="P5549_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRW11764.1"
                     /translation="MIPFSKLERYEGNETLFDLVTMSIVSSLVGEPLHIHAEGLRGTG
                     KTTIMRAARGIVPNITRIKNCVYNCDPEHPHCPHHRHLSPEEIAAIGVEDVPMPFLEI
                     SHSAKVGTIAGSIDLARLTDTAHPEAQLLPGIIPQAHRGIIFIDEINRLADTSPEITD
                     ILLDVMGNKPGHLQIEEAGLPVVDVEVSVSVWAASNPDEDPGPLEEIRRQLSDRFDMV
                     CYMGRPTSVDVLSQMLKENSHSQKIKKGKEMHLSAREKDDVYRQNIIKWAGQYEKADL
                     PDFLRNYIARLYVKHNLESIRAIEAMQQGAILYSTIKNRDQAMISDVTHMIPQVLKHR
                     VDGDTLIKMINDVEVRGSRDGILNFKNKKNNPEKKKEDTEYFNTAGRPLKDLGRNELI
                     NTEKTLAPKEG"
     gene            11755..13530
                     /locus_tag="P5549_01435"
     CDS             11755..13530
                     /locus_tag="P5549_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRW11765.1"
                     /translation="MDSGSGNISTATSVGNYLNLYLGQGQGVRLGDSIVVSRKAHARM
                     PEKVIKGQIKILINKDAGKTYLDYVDADQVVHIDVYHKEGEIDPRDVAKSIFWALDNH
                     LLEDEPQLKYAEDERFRIVADYANKVFLTTNSGKGLLYDLFQDKLTPYSEGEEGFDHV
                     HILARFNRDEYALVYVISEAVEAAILASDVKLAKVRNISHGKEKKQEENFAGNMKLPW
                     KKFKGRIASQLPDKENVNELIMKLAEKFGGVEEIEEWMDCYSTNIFKRKGVEYQKKKW
                     GDVEHYVEQLEELGLIEPGLTGRKLSKRGVFLHDYIIKHKIELETEIRRNMRKVPGGG
                     ANRFRKYGKSESKHSQVEFTNYNRTVNNSEKNWSGNLAVPETVIQAKKNNFLRGEKHF
                     TLQKEDLHFYDKKTYVPIDICLLIDASGSMAGEKRQAACFLAEHLLLSGKEKIAVVTF
                     QEHSSEIVVPFTRNQKQLSKGLATINPAGLTPLASGIMTALKLIKSTRLRNPLLVLIT
                     DGIPNVPLWTMDAKTDAITAAAQLKDTKVRFICIGVESNRIYLEKLANKAGGSLYLVD
                     DLNKENLINIVRNEKKAISTSELQS"
     gene            13696..14301
                     /locus_tag="P5549_01440"
     CDS             13696..14301
                     /locus_tag="P5549_01440"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRW11766.1"
                     /translation="MRNTMIVVLAMIVLGICLLSGGCSRPDQSVISEWQELLKFPAGD
                     QQAAKGGNNPDIAPLIETGTGMTGETITVSLYFADATRQQLVREDRAIAKVEGIARET
                     VEELLAGPANAESTEVFPAGTRLLDINLKPDGLCIVDLSSEACQVTDRSQAQLMVEAI
                     AKTLGQFPAVQEVTFMINGEKIDSIGGYVDLSAPIGTDYQA"
     gene            14456..15256
                     /gene="murI"
                     /locus_tag="P5549_01445"
     CDS             14456..15256
                     /gene="murI"
                     /locus_tag="P5549_01445"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRW11767.1"
                     /translation="MNDTRPIGIFDSGVGGLTVVRSLLTQLPGENFIYFGDTAHLPYG
                     NKSQAELFQYAQDIVGFLLQQQVKAIVVACGTHSSITLPEMEDRCPVPILGVVKPGAA
                     AAVAATRNGRIGIIATQASVNQGSYRQQIVSQGEYQVFASACPRFVPLVEAGHFDGAE
                     VEAAVAEYLSPLLANEIDTLVMGCTHYPFLAEAIAQYAGPGVTLVDPAIDTIKTLAAV
                     LHRDHLLGSAALGQGRFFVSGNDQSFFHAGSMLLQHQLIQKVEKISFD"
     gene            15268..16251
                     /locus_tag="P5549_01450"
     CDS             15268..16251
                     /locus_tag="P5549_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HRW11768.1"
                     /translation="MMNHTIGFTTSIPVEIILAAGRVPVDLNNIFITSGQAAQWVEKA
                     EEAGYPRNSCGWIKGLYTAGLLTQPEAVIAVMHGDCSNTQALMETMQLRGIPIFPFAY
                     PYDRSADMLTLEMEKLMRVFQVSWTQVEDVRRDLLPLRQLCQELDRLSWQENVLTGAE
                     NHYYLVCSSDFNGDPQKYARDLEEFIARAQLRRPFQDEVRLAYIGVPPIFTNLYEYVE
                     SLQARVVYNEIQRQFSMPFACQDIIDQYLQYTYPYGAFARLEDIQEQIHLRQVDGIIH
                     YTQTFCFRQIEDMVFREKLDLPILTLEGDKPGPLDARSKMRIEAFINMLKF"
     gene            16291..17055
                     /locus_tag="P5549_01455"
     CDS             16291..17055
                     /locus_tag="P5549_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRW11769.1"
                     /translation="MLGMDLGSRNVKIMEMQDHVITAQLSFDTIAFYRSYGKTGAGNL
                     RIDLSELGLAQNQPLVATGYGKVSVQIEGALHLPEIQAHVKGAVYQTGYQDFTLLDIG
                     GQDTKIVKVRGGRAVDFLTNDRCAASSGRYLENMAGVLGIDLEELSRYSLQPVELNST
                     CAIFGETEIIGKIVEGYPLAELAAGINYSLYRRFAGMLERLASDLIVVSGGVARNEAI
                     VNIIARESDRQVMCLPEAQFNGALGACIYGYEKFSK"
     gene            17071..17913
                     /locus_tag="P5549_01460"
     CDS             17071..17913
                     /locus_tag="P5549_01460"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRW11770.1"
                     /translation="MRQINVDTIRQAVKEMVMEANTRLPGDLQAALVSAKEKESSSRG
                     RQILEICLENAALAASEGMALCQDTGLVVIDLEIGQALQLLGGDLEKAVNQGVREGYL
                     AGSLRKSMVSDPLHRVNTGDNTPAVIHIHIVPGENLKITVLPKGAGSENMGQMAMLKP
                     AQGVEGVKEFVFRVVREAGGNPCPPIIVGVGIGGNMEKVALLAKQALMREIGQVHPDA
                     RLADLEAELLTAINQMNIGPQGLGGDTTALAVHIETYPTHIACLPVAVCIGCHSTRRL
                     SRIL"
     gene            17929..18480
                     /locus_tag="P5549_01465"
     CDS             17929..18480
                     /locus_tag="P5549_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRW11771.1"
                     /translation="MTKILSAPLAREEIEALQTGDEVLVQGTVYAARDAAHQKMTQAL
                     ANGQGLPVDLQGQIIYYVGPCPAPEGRVIGSAGPTTSGRMDVYTPLLLEQGLKAMIGK
                     GPRNEEVIAAMQAQGAVYLAAVGGAGAYLAQRIIAAQVVAYPELGPEALLKLEFKDFP
                     CIVAIDCRGNNIYAGNREEGASV"
     gene            18477..19202
                     /gene="rph"
                     /locus_tag="P5549_01470"
     CDS             18477..19202
                     /gene="rph"
                     /locus_tag="P5549_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRW11772.1"
                     /translation="MSRPEERKNDELRPVRITPGFVKNPDGSVLVQTGNTMVLCTAMV
                     EEKVPPFLKGTGTGWVTAEYSLLPASTDTRSPREAGKGKITGRTSEIQRLIGRSLRAV
                     VDFQALGERTIWLDCDVLQADGGTRTASITGSFVALSLAVEKLLAKGTLTQNPIVDYL
                     AATSVGIVNGEALLDLEYVEDSAAEVDMNVVMTGRGEFVEIQGTAEGKPFSRQAMDEL
                     LQLAEKGINELISLQKKILTGEQ"
     gene            19209..19814
                     /locus_tag="P5549_01475"
     CDS             19209..19814
                     /locus_tag="P5549_01475"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HRW11773.1"
                     /translation="MKTKLLIASRNQKKKNELQQILSDSAFALLSLDDLPPLPEVEED
                     GDTFAENAIKKAVTVARQTGLLTLADDSGLVVDALQGEPGIYSARYAGPEANDEKNNQ
                     KLLLALQDTPEQERTARFVCVIALAFPDGRVITVEGTCNGRIDRQSRGSEGFGYDPLF
                     VPDGYEESFAQLSAQVKHSISHRGQALELIKPYLQRINLEG"
     gene            19816..20289
                     /locus_tag="P5549_01480"
     CDS             19816..20289
                     /locus_tag="P5549_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HRW11774.1"
                     /translation="MLIAVVADTHGETDGVCQHLKQWKPDRLIFAGDYYRDGEKIAQA
                     LQIPYHGVRGNCDRHDPRARDEEIIEIEKKKFYVLHGHLYGVKQSISRLYYRALEIEA
                     EVVIYGHTHDAHLEQIDSLWMINPGSASRPRFDSRGSYVLIDLQSESLLPRLVKM"
     gene            20386..21489
                     /gene="gcvT"
                     /locus_tag="P5549_01485"
     CDS             20386..21489
                     /gene="gcvT"
                     /locus_tag="P5549_01485"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRW11775.1"
                     /translation="MKQTALISMHRKYQGKLIDFGGWELPVQYAGILKEHHMVRQKAG
                     LFDVSHMGEIEVKGDGAEDFVQKLVTNNVKMMADGEVIYTPMCYPHGGVVDDLLVYRY
                     SPQHYFLVVNAANTDKDYDWIKSNCPSSLACSNVSSQYAQLAVQGPQAQEILQKLTND
                     DLEQIEFFHFVPAMQIAGISCLISRTGYTGEDGFELYVAPGQAEQLWEAVLQAGGEEI
                     APIGLGARDTLRFEAKLPLYGQEIDQDINPLEAKLGYFVKLDNDDFIGRDVLRQVKEN
                     KPARTQVEFVMVGKGIPRSHYEVQKDGQKIGWVTSGGYSPTLDKNIGLALIDNRGYKN
                     GEQIDIIIRDKPVAAQIGEGLFYRKKTKPKSKA"
     gene            21532..21927
                     /gene="gcvH"
                     /locus_tag="P5549_01490"
     CDS             21532..21927
                     /gene="gcvH"
                     /locus_tag="P5549_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRW11776.1"
                     /translation="MNIPENLYYSNEHEWVRVEGENAYIGITDFAQEHLGDIVFVELP
                     EPDTDVSAGEQVGVIESVKAVSSMFSPVSGTIAEINEALEDTPELLNEDPYQNHILVV
                     RMSDPDDLDALLTPVKYAQLCEEEERGGK"
     gene            21929..23248
                     /gene="gcvPA"
                     /locus_tag="P5549_01495"
     CDS             21929..23248
                     /gene="gcvPA"
                     /locus_tag="P5549_01495"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRW11777.1"
                     /translation="MAYTPNSQRVAEEMLHSIGLESMDELFADIPREVKLDQLLNLAP
                     GLGEIELRQYMQTLAGRNLNVDQYPCFLGAGAYDHFVPAVVDQILQRSEFYTSYTPYQ
                     PEISQGILQAIFEYQTLICQLTGMEVSNASMYDAGTALAEACLMACEASKRKQILVPE
                     TIHPEYQKIMDTYSISGKMNCQAVPGRNGVTDVEQILALIDKETAAVVLQTPNFFGNL
                     EEIERIEQAIHQQKGLLIMVVDPISLGILKSPGEYRADIVVGEGQALGNGLNFGGPYL
                     GFMASSKKYMRKLPGRIVGQTLDFKGRRAFVLTLQAREQHIRREQASSNICSNQALNA
                     LAASIYLSMVGAQGLRMIAERCHQLAVYASNEFAKIGLSLQYDQPFFKEFALKVKEPG
                     RVNRLLLDNGIIGGYELEDALLLAFTEKRSRMEIDRLVALIGGVEHE"
     gene            23241..24695
                     /gene="gcvPB"
                     /locus_tag="P5549_01500"
     CDS             23241..24695
                     /gene="gcvPB"
                     /locus_tag="P5549_01500"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRW11778.1"
                     /translation="MNKILFEKNQPGSNGFSLPPLDFPADALSSTIPAAFLRQAELDL
                     PELSEVEVVRHFTALSHRAYGVDDGFYPLGSCTMKYNPKINEWAARLPGLAAIHPYQA
                     QETVQGALEILYKADKAFSEIAGMDRFTLQPAAGAHGELTGVMIIKAYHRHHGDEQRT
                     RMLVPDSAHGTNPATANMVGYEVVEIKSNDLGLVDLDDLRSKMNRETAGLMLTNPNTL
                     GLFEQDIQAIAEIVHEQGGLLYYDGANLNAIAGVARPGDMGFDVVHFNLHKTFSTPHG
                     GGGPGSGPVGVKEHLSAFLPAPLVECQDGRYWLDDDRPLSIGRVKEFYGNFSVIVKAY
                     CYLLTLGAAGLRSASEHAVLNANYLRHHLASDYQIPFNRLCKHEFVATSQNQMEAAHI
                     STLDIAKRLMDYGYHPPTVYFPLIVKEAMMIEPTETESRERLDEFIEALRSIAREARE
                     NPEMVKNAPHNTVIVRVDEVAAARHPLVKWEAEA"
     gene            24692..26071
                     /gene="lpdA"
                     /locus_tag="P5549_01505"
     CDS             24692..26071
                     /gene="lpdA"
                     /locus_tag="P5549_01505"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRW11779.1"
                     /translation="MKDLLIIGGGPGGYVAAIRARQLGMEVSLFEHDKLGGTCLNRGC
                     IPTKAYYKNAEVLRTMQHLSEYSIRCMDVQFNLALAKERKDGIVNKLVTGVEGLLQAN
                     GVEVITGHAEIIAPSAVRANGKEYTGQRILIASGSEAARPPLPGIDLPGVVTSTELLE
                     REEMPAQMVIIGAGVVGLELACIFNSFGSRVTVLEAADHILGTLDSELVKRMNVFLKR
                     QGIDLHTSCRIEEIQSGQDQLMVLAQGKRDRVRVEADLVLVAAGRKAATQGLYLDELG
                     IVNERGFIRVDENFESSVKGIYAIGDVIGGQMLAHVASEEAVVAVERMSGIDSQVAYH
                     AVPACIFTFPEIAAVGMSEEQAKQAEIAYKVGKFQFAANGKAMTMSETDGLVKVIADQ
                     EERIIGVHILGPHASDLILEATLMVKNHMQWEEIRGTIHPHPTLGESLAEAILDLKGE
                     AIHLLPRRK"
     gene            26223..27221
                     /locus_tag="P5549_01510"
     CDS             26223..27221
                     /locus_tag="P5549_01510"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRW11780.1"
                     /translation="MIHIINPSTDPYFNLALEEYLLLDPDHQEEYFMLWQDRPVVVVG
                     RNQNTLKEIDPAAVKSRGVEVVRRLTGGGAVYHDLGNLNFTYIINDEQHIGFDFARFT
                     VPIITTLAQLGIQAENSGRNDITIKGKKFSGNAQYRWKNRLLHHGTLLFDSNIEAMTT
                     VLNTDPAKLVSKGVDSVRSRVTNIREHLPNEMTLPQFRSALLTACKAMGLLEAEQALQ
                     EEERQAVAGLQREKYQTWEWVYGSSPAFNVHHQAAFDWGKVEIGLCIENGLISDCRIY
                     GDFFSLADVNQLCAGLQGVPYRAETLHRKWAELDWQSCFPSAQTDELKAMFLKDVP"
     gene            27261..28172
                     /locus_tag="P5549_01515"
     CDS             27261..28172
                     /locus_tag="P5549_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW11781.1"
                     /translation="MQLQHITGNSYFLEGDNCIGVYVFADRSCLLVDSGASESQAKKI
                     LHCLTEAGLQIDTIFTTHAHVDHCGGNQYLQSQCQCRVMASSLAAAILENPMLGPAML
                     FTAYPLRVLRSRVLMAAPCKVNQTVSPGPLLIQNELFQVYDLPGHSLGQTGLATPDGV
                     LFLGDSLMHTQMLQEYPFLYMVDITRQRETLAFIGSHQWPWVVLSHGGLVEDLDDCVG
                     QNQARIAIIAESILALLDQGRSHEEILAALLRQYELSANSGQYYLVSSTIAAFISYLS
                     EQKLIRNRVEQGVMKFYRISLRDRQEE"
     gene            complement(28206..28580)
                     /locus_tag="P5549_01520"
     CDS             complement(28206..28580)
                     /locus_tag="P5549_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HRW11782.1"
                     /translation="MNKSRINLLLDAIMLLGMMALVGTGYVRKYILLSGSAAKAAFGG
                     KVDMLLLGIDRDGWAVIHLYLGYFLLFLLFWHIYLHWQQVVAIYRKWIPNKNIRWTIT
                     TVFILLSLVLLLFPFFIQPVIL"
     gene            complement(28700..29374)
                     /locus_tag="P5549_01525"
     CDS             complement(28700..29374)
                     /locus_tag="P5549_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11783.1"
                     /translation="MAGIILVLSAHANAGQTTITVNLASALARQGHRVLIAEKGENKK
                     LRAWLGFDRAADRPMPSSGQMMPGTVATRIGTDFWMMKDAERILPEIAVDYEYVFLLP
                     ATSAGCENLLPRCDRILVCCSLEEKDTARQVVDLDQHLSSLTGRPQPIDLVVMNKINT
                     KEWHNNLECFSLLADHFGEERIADPLPHCERIHDLPLDGRTVWELSQENLQDAFRRLT
                     EIIAAW"
     gene            29588..30310
                     /gene="prxU"
                     /locus_tag="P5549_01530"
     CDS             29588..30310
                     /gene="prxU"
                     /locus_tag="P5549_01530"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084691462.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:29837..29839,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HRW11784.1"
                     /translation="MPEEFKPGCQRPPIKKVNVQTEEIATTELITKEAKQSMVKVGKP
                     APDFTAAAFGQGKFVNTSLSEYKGKWVLLCFYPGDFTFVUATEVSAVAEKHAELQELG
                     VEVLAVSVDSVFVHKMWNDHELSKMINKDIPFLMCSDQDGSIGKMYGIYDEDSGTETR
                     GRFIIDPDGIIQGFEVLTPPVGRNVSESIRQVKAFQLVRASQGTEATPSGWKPGKTTL
                     KPGPDLVGNVWKVWKVSEAFED"
     gene            30444..30698
                     /locus_tag="P5549_01535"
     CDS             30444..30698
                     /locus_tag="P5549_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11785.1"
                     /translation="MKAKPNPTIIQENTLQVMADTLTRIDSLKCFHIPIVLSVIEQYK
                     VAGIEGYFMEQQELQLQKLLSMLVQLEEKLARLQQRLLIS"
     gene            31011..31916
                     /locus_tag="P5549_01540"
     CDS             31011..31916
                     /locus_tag="P5549_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRW11786.1"
                     /translation="MKKSLQRIKRLLALMVLALVLLPIAGCGNPAPETVSSGAGKIKV
                     AVSIVPQASFVAAVGGDLVEIVTMIPPGGNPENYAPNPRIMEQASDARIYFAIGVAAE
                     QNGILPRLKQLSADMSVIDLASRADAVYPALEIVPGEKDPHRWMSPRRVSIMVEMIAD
                     ELAQADPEHAKIYQGNMQNYQAQLEKLHQEIEASLADADHRSFIVYHPSMGYFADDYG
                     LTMIALEEEGKAATARHIQQVIDQARQENIKAIFYQAEMDGKQAQTLARELGGEARLI
                     APLAPDYIDNLRQTAQTFSHVLKTE"
     gene            31996..32766
                     /locus_tag="P5549_01545"
     CDS             31996..32766
                     /locus_tag="P5549_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW11787.1"
                     /translation="MSNEMAICFEDVSTGYGNVLVLEDINFCIQAGSFTGIIGPNGGG
                     KTTLIKLMLGLMEPWRGKVTIFGEKPQDMRHLIGYVPQAAHINQRFPISVMEVVLQGR
                     LGEKPFFFHRYRQYDWDKARWCLQKLEAADLANRQIGELSGGQWQRVLIARALAAEPR
                     LLLLDEPGNGLDSNASGKVYELLEELNQEMTIVMATHDTMAISTCVRDIACINHRLHY
                     HGEPELSPDLVMQLYGCPVELIAHGVPHRVLHQHGGQQ"
     gene            32763..33593
                     /locus_tag="P5549_01550"
     CDS             32763..33593
                     /locus_tag="P5549_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRW11788.1"
                     /translation="MINALLQYGFLQNAVMASLLASLACGIMGPMIIEKKLLMMSGGI
                     AHTAFGGIGLGYFLQIEPMYTGLGFSLLSAVGIAQIQRRALVMPDVLIGMFWPLGMAL
                     GILFISFTPGYPPSMESYLFGDILMVSGTDLLVMTSLDGIVLATILAFYHLLQAYLFD
                     EEFSQTRGLPVALAEALTYAMIAITVVVLIRVVGIILILALLSVPPALARLFRDDLPG
                     IMVLSFAIGVFLCLTGLALSYHYNLASGAAIVILAVIIYTLFQLLDKTLLAFVRQRQL
                     "
     gene            33645..34079
                     /locus_tag="P5549_01555"
     CDS             33645..34079
                     /locus_tag="P5549_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRW11789.1"
                     /translation="MDAEQLCARLAAKGYKLTNSRRKILHSMAAQTDWVTAKSLHDKL
                     QQQNIHIDLSTICRNLDLMYAMNMLCRVDKERNGTFAYRLQDMQNHHHHLICLSCGTI
                     VPLEYCPLERLPERQTQGFSHLECQFEVYGHCQACQRAIRKS"
     gene            complement(34118..34285)
                     /locus_tag="P5549_01560"
     CDS             complement(34118..34285)
                     /locus_tag="P5549_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW11790.1"
                     /translation="MYVDNSCIGCGICVDTCPVNAILIIENKASIDSSLCLECGLCAD
                     ICPIGAIHEKA"
     gene            complement(34378..34695)
                     /locus_tag="P5549_01565"
     CDS             complement(34378..34695)
                     /locus_tag="P5549_01565"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRW11791.1"
                     /translation="MPRGDRTGPLGRGPASGRGMGYCSGYANPGYLSNTPGRCFGWAR
                     GMRGFGGRGRGMGWFNQPYYGVNANDTVAGLEERARFLEEELADIRRTISAAKTGKNI
                     DNV"
     gene            complement(34748..35143)
                     /locus_tag="P5549_01570"
     CDS             complement(34748..35143)
                     /locus_tag="P5549_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11792.1"
                     /translation="MARYLHYILTVMRIAISCWQNCIAPVFDYSKNLLLIDIVNNKVI
                     SSETMRLDIELPFQRAEELSRWATEVVICGAISRTQVMALQGRNIQIIANITGSLEEI
                     ISLFLTGQLAGTGIMRQHRQRRRRGQGNL"
     gene            35295..36662
                     /locus_tag="P5549_01575"
     CDS             35295..36662
                     /locus_tag="P5549_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW11793.1"
                     /translation="MKIENLKFNNNNYEIILDSINEGVFTVDQDWRITSFNTAAEKIT
                     GISKKEAIGHLCYEILRANVCETNCALKHTFLYGKPIINSTANIIDNCGRKVPIRLST
                     AILRNEIGEIVGGVETFQDLSYIEQLQKQLESSYSFEDIIGRSTRMIELFSILPQVAE
                     SSSTVLLNGASGTGKELFARAIHNLSPRHNKRFVGVNCAALPDTLLESELFGYQAGAF
                     TDAKRDKPGRFLLADGGTLFLDEIADMSTAMQVKLLRVLQERVVEPLGSLEPIPVDVR
                     VIAATNRDLNEMVRNGLFREDLYYRICVVHMQLPELKQRREDIPLLIEHMIYKFNRLQ
                     NKNIAGVSDEVMAVLFNYDFPGNIRELENMIEHAFVLCRGELIELQHLPAEIRALCKT
                     TVKELPTLNLDSMEKHLIIEALQKHQGHRKLAAQDLNIDVSTLYRKMKALDIQYQKEN
                     NENKD"
     gene            36678..37628
                     /locus_tag="P5549_01580"
     CDS             36678..37628
                     /locus_tag="P5549_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW11794.1"
                     /translation="MRCFGPVPSRRLGRSLGINNIPPKICSYACVYCQQGFSSHMQAG
                     RQAFHDPDELFSEVKERVERMERNGEQIDYLTIVPDGEPTLDVNLGELIRKLQSLKLK
                     TAVITNGSLIDHPQVRRELAEADLVSLKVDAVNEDVWRKVNRPLRQLHLEKIQAGMLA
                     FSQVYKGRLISETMLIKDVNDTQAECQAIAGFLTQLKPAVAYISVPTRPPACKWVNKP
                     DEEKLHLAYQIFAKDLSKVEYLNEFEGSDFSFGGDVARDILDTCAVHPMRQDALQTLL
                     GNAGADWQTVEDLISSKQLVELVYDGNKFYMCKLYEGYKR"
     gene            37722..39065
                     /locus_tag="P5549_01585"
     CDS             37722..39065
                     /locus_tag="P5549_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW11795.1"
                     /translation="MKSTNVLIIGGSAAGLSTANSVKTWHPNKSVTVVRDVAYTVVPC
                     GIPYIYGTLKAVEKDKIPDEGFLKKGIEIVVSKVSSIDRSQHLVTLADGETISYEKLV
                     LATGARPVVPNIPGAQLENIFPIYKDPDYLQHLDDVLQHNKKVVVIGGGFIGVEMAEQ
                     IRLKGDAYNVTLIEALPHCLAVACEEEYGQKIEMELEQMGIKVMTNTLVEAFTGQQKV
                     EGVVLKGGGSIAADIVILGIGTLPNIALASQAGLQVDEKEGMIVDEYLRTSDPDIFAC
                     GDCASKFSSINGQPVIIRLASVAASEGLIAASNLFQLNRKSPGALGAFATKVGNTAVG
                     AAGFTERMCQEDNIDYYVGKMVSPDRHPASLPGCTMQTEVKLLFDRRDDRIIGGHVLG
                     GVQAADMVNILALAIQQKLTAAELAIMQYATHPLLTGSPLVYQVMWAAENAIAKK"
     gene            39156..39638
                     /locus_tag="P5549_01590"
     CDS             39156..39638
                     /locus_tag="P5549_01590"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HRW11796.1"
                     /translation="MKLTDAEVIELLQYSFVRIDGAWFMGLAKTHGIETAWDADVEAW
                     KQYSYVFGKKLRNDYIQEPVWPESFLEAIEILSKVLKIEGREVSLEGERIVVRVTDCE
                     TQKAIAKANIADCGIVTIHTYQGLVRGLFEKDVEIEVEHTQNLNHGDPCCEVVIQRKS
                     "
     gene            39687..40517
                     /locus_tag="P5549_01595"
     CDS             39687..40517
                     /locus_tag="P5549_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW11797.1"
                     /translation="MNVSVTILVENTTPIPGFRGEYGFAALITVDDKKILFDTGSEDA
                     LIKNAAAGGIDLAQINDLIISHGHFDHTGAVIPFLQTAPGKKVYAHAGIFIPRYTVWG
                     NFRKAIGVPFDSQEITRNGAQFVITNDFTEIHPGVFVTGEIPRKTHYEDVGGSFYVEV
                     GDRLVEDHLYDDMSVVINHPEGLIIISGCAHAGIINTIEYARQQTGQKKVRAFIGGTH
                     LAGASEERMQETIKALREFDVEQIIACHCTGFEATMKLRNELGDRVMKGETAMNFQYQ
                     "
     gene            complement(40605..41252)
                     /locus_tag="P5549_01600"
     CDS             complement(40605..41252)
                     /locus_tag="P5549_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HRW11798.1"
                     /translation="MIIIDKDKDVFQPEKSQPPIGNDCSICQKQRCKKGRDCYPEINQ
                     GIIQIYKKPDNYKLVKASAAIEATTYQQATRLEEIRLFAREMSYSKIGIATCIGLIAE
                     AQMIAAYLKKDFDVTTVICKNGGLLKKQLELEQIDPSKDEVMCNPIGQALLLNQKQTD
                     LNLICGLCVGHDILFSQYSEAPVTTIIVKDRVLGHNPAAVLYTSYYRKKVLDLWK"
     gene            complement(41324..41527)
                     /locus_tag="P5549_01605"
     CDS             complement(41324..41527)
                     /locus_tag="P5549_01605"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HRW11799.1"
                     /translation="MPNKDGTGPMGQGAGTGRLKGGCAAGRGNNKNNSPGKGLGRRNS
                     GNNGPQNGGRGQGANNPNNTSNK"
     gene            complement(41560..42414)
                     /locus_tag="P5549_01610"
     CDS             complement(41560..42414)
                     /locus_tag="P5549_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW11800.1"
                     /translation="MKEILFISGKGGTGKTSLTSSFIALADNCIACDYDVDASNLPIL
                     LKPNNIVGHDFSSGYSACIDEEICTSCGLCKEVCRFEAINEDYQIIPLHCEGCGFCAR
                     ICPVEAIKMNPRPSGHWFRSEFKPGQFLFFADLRPGEENSGKLVAQVKTQARQKALTD
                     NVSLIIADGPPGIGCAVISSMVETDLIVLVTEPSLSGFHDLKRVYALIQLRGIKSVLI
                     INKWDLNPETSQTIEDWATAQGVCVAGKIPFSTTIADCIATARIPATQPQIRNFIFPV
                     WENILQQL"
     gene            complement(42411..43253)
                     /locus_tag="P5549_01615"
     CDS             complement(42411..43253)
                     /locus_tag="P5549_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW11801.1"
                     /translation="MQIAIASGKGGTGKTLLSTCIIKVLADKNAVLMDADVEEPNANL
                     AFLQPISATTTVYRPVPQILPEYCTYCGLCAEICEFNALVVLADKVMVFPELCHSCGA
                     CSYLCPYAAIDEIEHPIGVIEETPLQTSGTLLTGRLNIGEAQSPPLIKAVKNEQLSAA
                     IRLVDCPPGTTCPMIEAIRDSDYCLLVTEPTPFGTHDLELSLEAVKMLGMQGGLVINR
                     WQGNDGELLDLSERWGFPILARIPFSAELARAYMQGLDPFAAMPALRGIISDILHRIK
                     EVSA"
     gene            complement(43267..43614)
                     /locus_tag="P5549_01620"
     CDS             complement(43267..43614)
                     /locus_tag="P5549_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRW11802.1"
                     /translation="MLIAIPAEANMVCPHFGHCEVFAMYDTDTKALKSLPNPGHEPGF
                     LPVFLKKQGVHLVIAGGMGGRAQELFAASGIELIVGVSGSIQDVIALYEKGELKSTGA
                     VCSDHSHAGDCHH"
     gene            complement(43747..44100)
                     /locus_tag="P5549_01625"
     CDS             complement(43747..44100)
                     /locus_tag="P5549_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRW11803.1"
                     /translation="MSGKIAVCSTGSTPSSLVDERFGRCAYFMIWDPASKEFTALPNT
                     VTDSAHGAGTGAVQTLLENKVNLILSQRIGPKAFAVIEQAGVKIFSGITGKTVAEALR
                     SYENGELQELLTPSN"
     gene            44452..46272
                     /locus_tag="P5549_01630"
     CDS             44452..46272
                     /locus_tag="P5549_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRW11804.1"
                     /translation="MSNVFEILAEMKAIGLKLVLVGNPNVGKSSVINLLTGSQFFASN
                     YPGTSTEIEKAVLKINQVEFAFYDTLGVYSLYTDREEATLTRTLLEEQNYDLIINIVD
                     ASNLERNLVLTYELLERGYPLLVLLNQVDRTRELGIQIDVAELARILQRPVIAFSATN
                     NEGVSEFRQFLEELGKKPDAGREKEFTRQGLPRIVLMEDTSCSGNCGSCSTSRESCSI
                     DLDLQLAEKARKTSQAVSTFKTPAKHSLINDVQFFIDRPYVGTISLLLLAYLALVVLT
                     KFISFSEGPISQLLEPLSNMLHNWILAIMPAGFLTTVLAKAIPEGLVIPFTIIMPAML
                     MVSLLMAVLEDTGLLPRYSVAMERFGQLFGVSGQAVIPLSLGFGCRTPAVMATRILPN
                     DAQRFIVITLLSIVIPCAATVGVIVMISAKFEASLTVIVLTMLVVMTLLGLLLSRLSP
                     REEAFIYELPPLRVPRRKNVWSKIKLRFAGFFTEVLPLLLVMSIAIRALIESHLLEIF
                     NSMDALTKVIFGIPAEAFVAVLITIFQRYLAPLVLLNLDLSAREATIALSMIALSLPC
                     LPVMVVTVREMGFSSLTKILLMGFITSALVGISLNLILPF"
     gene            46285..46539
                     /locus_tag="P5549_01635"
     CDS             46285..46539
                     /locus_tag="P5549_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRW11805.1"
                     /translation="MLATEMKKGQKAMVRSFGRGRGMERRLFEMGIMPGTEIELLEKH
                     PFKGPLLFQVGQARIVLGRNIAAHLEVEITEISLPSHFGS"
     gene            complement(46553..47299)
                     /locus_tag="P5549_01640"
     CDS             complement(46553..47299)
                     /locus_tag="P5549_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRW11806.1"
                     /translation="MARESKCRRVAFLPPVKAYHPLGVPLADMEEVIFKVEEIEAIRL
                     KNHLNLEQEDCARMMEVSRPTFQRILTEAYAKVADAFTNGKAIRIEGGSYCLGQGQCR
                     RRERQLDYMEGCRFYDSGLELSATDNPPVHQTKIAVCAESTDLSDPVRERFGKCHCFW
                     IWDPASQAFSLLDVKAANTEQGQGTIGAKALVQAGVKAVIVSRIGPKAFLILQRAGID
                     VFTGANGKSITEAVQMLQHQQLNKADSPNG"
     gene            47615..48391
                     /locus_tag="P5549_01645"
     CDS             47615..48391
                     /locus_tag="P5549_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRW11807.1"
                     /translation="MRIEEGPMLVGSLNKINKVIAVMSGKGGVGKSSVSSILAASLAQ
                     NGYQVGILDADITGPSIAKAFGVKAGSCTVSEYGMNPPLSKGGIKTLSINLLLENEDD
                     PVIWRGPLIAGAVKQFWEETNWGHLDYLVVDLPPGTGDAALTVLQSIPVSGVVIVSSP
                     QDLSLMVVRKAIKMVQKMEIKILGLIQNMSYVECPDCGKKIELFGNSSGDKVSDDMGI
                     AYLGEIPWDNQINKLMDAGEIEFYEKINRLEAIVKIIELA"
     gene            complement(48528..49820)
                     /locus_tag="P5549_01650"
     CDS             complement(48528..49820)
                     /locus_tag="P5549_01650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRW11808.1"
                     /translation="MKDVFVVEAARTAVARAGKQSWFTNVRADELSAIVFKELRKRIG
                     CEDKAKQAELIDDIVWAATANAFMEQGLNQGRIAWLLSDGSYDVPGCTVDRFCASGIN
                     SISFATATMMAGWGGDVQIAGGAQHMSHIPMGAGADVHPDLGKYMDLRSINMGHTAEV
                     VARRYGVTREMQDELACASHAKCAAAQAANGGLGKNKDAIIPIIAKVPAKGAKGEVKR
                     DGAYVASDYLAKAAAEKGEELVEFCVARDQGLRAETTMETLAAMAPVFAQDDKATVTA
                     GNASQINDAAAGVVLATADGAKALDKKPVMKLVNYAVIGCDPDVMGIGPSLAIPKVLK
                     RAGMTMDQIGLWEVNEAFASQAHYCCNMVLNLPKDRTNVWGSGISIGHPLACTGARIA
                     CDIFYMFKDPDFADVDYIVESMCIGHGHGAAAIWERVK"
     gene            50368..50649
                     /locus_tag="P5549_01655"
     CDS             50368..50649
                     /locus_tag="P5549_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRW11809.1"
                     /translation="MKEEINDQEARKNVLVRLRRIEGQVRGVQRMVENEAECSEIMNQ
                     IAAIKSAVNHVGLLVFESHARECIARSLDEKDNEQSFEEIVKIMGRFIK"
     gene            50693..51019
                     /gene="trxA"
                     /locus_tag="P5549_01660"
     CDS             50693..51019
                     /gene="trxA"
                     /locus_tag="P5549_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRW11810.1"
                     /translation="MSSVKAVNTESFNNEVLKANKPVMVDFWAPWCGPCKMLGPIVDT
                     LAEENLAKLDVAKVNVDENKSLAAQFGVRGIPTLIFFKDGQEVKRLVGAQGKPQLQKM
                     IDEVIG"
     gene            51218..51913
                     /locus_tag="P5549_01665"
     CDS             51218..51913
                     /locus_tag="P5549_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRW11811.1"
                     /translation="MAEITVVTFNIRHGQGMNGKVDLAKTEQVLARCQADIICLQEVD
                     RGRMRSGFQDQAARLADTLQYQHVFGPVPNDRSQSYGNAILFRHKMLQQNNYPLPAEV
                     PERAMLSIELEIGAQNLVLFNTHLGLNRRLRSEQIEQFIIPLMDRIGLPAILCGDFNE
                     SPEDEGAARLLQCWQDSFVANRTPICCSYPADHPRQRLDYILCNQFCQVREYAIVESL
                     ASDHRPVLARIAF"
BASE COUNT        13728 a        11785 c        13477 g        13013 t
ORIGIN      
        1 atttttggca tcatgatatc catgatcacc aaatggatag ggttctcttc cagtagcggt
       61 aaagcctcca gaccattggt ggctttaaac acggtataac cttcgttttt cagatagatg
      121 ccgatggcat ccaggatctc tttgtcatcg tcgcatacga ggatattcat gaagtatcac
      181 tcctgttttg gttattatcc aatgaaacat cacacctttt atcatccaac tttactgtta
      241 tgatagcaat caattcttac gaaaccagcg cgaaaaatct taagaacttc ttaagattgc
      301 ccaaagttct gcggaaacaa tcaatcattg cccgggccat tcagctgctc acaaagcttg
      361 tgggagcttg cctgaaacgg gttgagtctg ataaatatgg gtgtgatgag gagagatttg
      421 gggccgggag agcatgcgaa ggctcatgga aaggaaaaag gccaggaagc tggatatcgt
      481 cctggctata ccaatccata tttatgcaga tgacggtaca gggtctggcg ggagatccct
      541 agttcctggg cggttttctt tttattaaac ttgttcttgc gcagcagttc ctccagtagc
      601 tggagttcct cggttgctgt gaaatccggg actaatacat cactgagagc ttttggcagg
      661 tgatcagctt ccaaacccat ataattgaca ataaaatcat tgtcgatttc cggcccgggg
      721 gctagttgaa cgcatccttc caaaacattc tgcaattccc ggatgtttcc cggccagtca
      781 tagctgacca gcgtttgcaa ggccagggga ctgattcgat ataattcctt gccgtatctt
      841 ttgcagatca gttttagaaa gtgactgacc agcggggcga tatcatcacg tctgtcccgc
      901 agtgatggaa tattcagctt gattacaccc aaccggtaat aaagatcctt gcggaagcga
      961 ttgcgggcaa tctcgtcttc cagattttta ttggttgctg ccatgatcct cacatttacc
     1021 ggtatgactt gattgccgcc caaacgcata acgctttttt cctccagcac ccgcagtaag
     1081 gtggactgca aatccagcgg catatccgcg atttcatcca agaacaaggt cccctgattg
     1141 gccagctcaa atttgccgac attgccgcct tttttggctc ccgtaaaggc ccattcctca
     1201 tagccgaaca attcgctggc aatcagttcc cgcggcaggg ccgcacagtt gatcgcgaca
     1261 aaaggattgt tttgccgggg actttcattg tgcatcgcct gggcgataat atctttgccg
     1321 gtgccgcttt cgcccatcag cagaacgttg gagttggact gggcagtggt acgggcgatt
     1381 ttgataatat cccggaactt ggggttgctg ccgattacat cgtcaaaggt catttgtgca
     1441 ccacgaccga tccatttgcc gacgatgttg ttaatccgct cactttcccg caggattata
     1501 accgaaccgg tatcgcccag aatggaacca tgcagcgggg tacaggtaat atgacagcgg
     1561 atttgattgt tgccattgta caggatcacc agatcatcgg ttaccattct gccctgattg
     1621 acaatattga taaagaattg attttccgga ttttgaccga aaagatcgtg tatgtttgca
     1681 ccgaccatat tggcagtgcc aaaaccaaga attttggaac acttgttgtt catgtaggta
     1741 atcaaaccat ttaaatcaac caccatgacg ccatccgaca tggaatcagt gatgacctgt
     1801 tgatacttgg agatgcgatt cagggccatc ttcgactgga tatggtcagc cgcggcgact
     1861 gccatgccca gcgtatgata actgacgcgc tggtaaggag ccaccatgcc gatacagccg
     1921 atcaccttgc cattgtcaac gatgggggca aaagacgaag ctgcggtgat ggcgtacaaa
     1981 caaaagtgtt cgtaggaata aacggaaatc ggtttgacca gagacagaca gatggaagca
     2041 ctgttggttc cggcaatttc ttcggtccag accgtaccct ctattaaatt ggtgcgcttg
     2101 gaccattcca gggtttcctt gtctccgtag tttaataaat taatgccgtt ggcatcattt
     2161 agtgcgacta aaaacccggt tcctttcaca aactcagaaa gacgttccat aaccgggatg
     2221 gcaatttcca ttaaatattc cgaattggtt ttggcccggc tgaattctgc atcagaacag
     2281 acatggacaa tttcccgcaa gttgcggtcc accttgtatt gataactgcg ttcccaggaa
     2341 tcataaatct ccgggcgcaa gccggtccgc aacgctggat cgttatgaag gttctgccat
     2401 agctttttta cttctttcca gggcacattg ctcttgtttt ccggatgcag ataatcgatg
     2461 aactcagtcg ctatttcttt gcttttgggg ttgactgaca taggcattaa cctccattgc
     2521 tgtcggtgaa gaaaagagtt tactctgctt cagtataaca tggaatatta catagtgtaa
     2581 caaggggctt tatcttagcc gggcggcatg ataattccac cgcgaagggg gtgcttttgt
     2641 tacacgttgt cccgctctgc ttctgtatct tatgtttttt tgataatcga ggcagagagc
     2701 atgatctgct gcgacaaggg gatgccgaca gaaacataca cgaatcgaaa aaattccccg
     2761 caaaatcata taaaagtaat gaataagctg gtataataaa aattaattag tattaagata
     2821 tttggcatgc gaaattatgt aaaaggagtt gagtaccata tttcagcatt taccgaaaag
     2881 tacaatgagc caggggtttt atcacacagc cagccacttg ccggacaacg tagcccaaat
     2941 gtttaatgcc gatctctaca atggtgacaa taacggtcag tttacctgga aagaaatgca
     3001 gcatcgtgtg gaaggcatcg cgggtggact tttaagcatg ggtctggaaa aacaacagcg
     3061 tgtggccatg atgtcgacca acagtcccta ctggacccaa gcagacgtag ccacgatttg
     3121 ttgcggcggg gtattggtaa ccatattccc cactctttcg ttaaacgagg tcaaatacat
     3181 cgtcaatgac tcagagtcgc gttttttcta cgccggcagc aaacaaatcc tggagaaagt
     3241 actgagcggt tgggacgaaa tgcccagtct ggaaaaggta attcttctgg atatggatta
     3301 tcgttccagt gattcccgaa tcattggtct gggagagctc attgaaagcg gcagtcaata
     3361 catgaaatcg cattatcagg agtatgaaca aatctggaaa agccttgatt tgagtgattg
     3421 ggcgacaatc ctttatactt cagggactac tggtgccggc aaaggggtta tcctgaccca
     3481 caacagcctc agtgagagag tggacggcac ccacaaatat tttgtacagg cgggtcatcc
     3541 ccttgatgag cgggatctgg tcctgtcatt cctgccttta tcccacattt ttgaccgcat
     3601 gtgttcacaa tggctggcta tataccgcgg ggcaacggtt gcttatgcgg acagtgctgc
     3661 cacgttgatg aacgatttac agaaatacaa ccccacctgg ttttcttgtg tccctcgttt
     3721 gtatgaaaaa atttatgcgc agtttaacca gatgatggcg gccagcgcag ccaagaaaaa
     3781 aatgttcgac tgggcctacc gggtgggcga agaagcgctg acttaccgca gagatgaata
     3841 cggacgctac aacatgcgcc aggattataa tctggaagcc agactgccgt cgggactgcg
     3901 catgaagtac aaagtggctg ataaattgtt tgccaaggta agagggctgt ttggcagccg
     3961 ttttcgcttt tccttctcgg ccagtgcggg gatcgatccc aaactgctga cgtttttcta
     4021 tgtgatgggg attccggtac tggaagggta cggctctacc gagacctgca gtgccaccaa
     4081 ttacaatccc atgtatgctg ccaaaccggg cagtgttggt ccggaggcca acggttccaa
     4141 ctgccgtctg gcagaagacg gggagttgga agtcagaggc ggattgttta tcggctatct
     4201 gaacaaacca gaggaagatg ccgctgcttt caccgaggac aggtggttcc ggacgggtga
     4261 cctggcgacc atggatgatg acggttatta ccggatcgtc gatcgtaaaa aggccatcat
     4321 ttgtctggcc accgggaaaa acgttgcgcc ttataaattg gaatcaaaat ttaccaccag
     4381 tctggtggtt gagcagattt tcgtgctggg ggatgaaaga aatgttatta gcgctttaat
     4441 cgttcccagt tttgcttatt ttatagattt ttatgaaaag caaaacatca cctatgatcg
     4501 cagccagctg atttataccg aaatgcaggg tgcccaggtt tgtattggcg caggccggga
     4561 tttcattgag cagcccatcc tgaaggatat gatcgcggaa aatgtcagac aggtcaacga
     4621 aaccctggaa ggctttgaac agatcaaaca gtacacgatt atcaaccaca actttacgga
     4681 agaaaacggc gagatgaccc ctaccttgaa actgaaaaag aaggctattc ttgaacacta
     4741 cagtgagatg atcgaggcaa tgtaccagcg ggaatactaa ttgcgtttaa aaaaagaatc
     4801 ataaatcatc atcccgtgtc gtaatttggg cgatacggga ttttttttgt gagcagtgca
     4861 agaagcgtgc ggatatattg gttaataaaa atttgacaat gccggagagt tctttaaaat
     4921 gaatgtatcc tggcaagatg cacaagtatg tgcacaatca tcatgaggag gaagttcatg
     4981 tccaattttc gcagggatct gttaaatccc gatctcaaag atatcctgga aacctttcag
     5041 ctcgataagg actacgtgca tggagagggg accacactaa cagattctga cggcaatcag
     5101 taccttgatt ttattgccca gtacggtgcc gtaccgtttg gctataatcc gtcctttgtc
     5161 tgggaagccc tggagcgtgt cagaaaacgg gccctgccca gtctggtcca gccttctctg
     5221 ccgggtgagg cactgcgcct ggccaatctg ctggcagagt gttccccggg cgatctgtgt
     5281 tattgcacgt tttgtcaaag tgggaccgag gcggtggaag ccgccatcaa actcgcccga
     5341 gctgccaccg gcaaagaaat gattatttcc accggcaaca gttttcatgg caagaccctg
     5401 ggagccctgt cggcgactgg caaggattcc tatcaaaccc ctttccgggc accggcaccg
     5461 ggttttgtca ggattccgta taatgacatt gctgctttgg aacaggcact gaacgattat
     5521 gccgggaacg tggccgcttt tatcgttgaa ccggtgcagg gggaaggcgg aattgtggtg
     5581 gcggcacccg gttatctgca aaccacacag cagctctgta acacccatgg ggtggttttt
     5641 atcgtcgatg aggtccagac gggtctgggc agaactggcc ggctttttgc ctgtgaacat
     5701 gaacaggtcg aaccggatgt gctgctgttg gccaaagcgc tggggggcgg cttgctgccg
     5761 ctcggagtct gtttgagcag ccctcgggtc tggaatgaag attttgccat gctgcacagt
     5821 tccacctttg ccaataatgg ggtgacgtgt gcagtgggta ttgcggtttt ggaaaagctc
     5881 ctccaggatc agcaggccct ggtcaaagaa gtgcagcaaa aaggcgatta tctgctggcc
     5941 aaaacccggg aattgcagga aaaatatccg caggccgtca aggaagtcag aggccaggga
     6001 ttgatggtgg gtctggaatt ccatcccctg gataactgcg gttcatttga tctggcttat
     6061 ctggccgatc agggcggctt taccgcttta ttgagcggat ttcttctcaa tgtctacgga
     6121 atccgtctgg cacccttcct gaacaactcc atgaccttgc gcctggaacc gaccctgacc
     6181 atcacctgtg aggagatcga ccgggttatc aaggcgctgg aagtgtgctg ccaaatcatg
     6241 tcctggcgtg attacagcaa actctaccgc tatttgctgg gcgaccggag tccggtaacc
     6301 catatcatcg actatcgttc ccagacacgg accattaagt cttcaccatt aaagcccggc
     6361 gaagcggctg cgcataaatt tgcctttatc attcattatc cggcgccgga ggatgtgatc
     6421 ctcaacaatc cttcttttgc ctccatgagc cgtgatgaat tatatcgctt tttagcgtgg
     6481 cagagcatga ccaaagaccc cggcattgtt tgtcacatgc cggccatccg ttcaaagagc
     6541 ggaaccatcg tggaaggctg gatgattggg gtgccgtttg gcggtcggga aatcatgcaa
     6601 ttaccccatg cccagacggt ggatatgatc gcccgggccg tcgacatggg cagagatctg
     6661 ggtgcggaaa tcgtcggcct gggggccctg acttctgtcg tgaccagagg tgggcgctcg
     6721 gtaaccggcc gcgatgtggc cattaccagc ggcaacagtt ttacaacctt gatgggcgtg
     6781 gaggcacttt tccagggagc agcgaagatg catattgaga ccatcgctgc ccggggcggg
     6841 gttgtcggtg cgaccggttc catcggtcgc gcctgtgctt tgatgctgtc ggaagaattg
     6901 tcacagttga ccttgtttgg caacccggac catcccgtca gcagcaacaa tcgtctgcaa
     6961 tcattggccc gggaaatttt cgtttatgcc agtcgacgta tgcagcaggg tgaaatgaat
     7021 ggcatcagcc tgtggctggc ccagatcagg cagttgctgc tcaatcgtgc ggaggagcca
     7081 gcgctcacct acctggcctg tttggacaaa gaagattttt ccatgacact gatggatgag
     7141 gtttgtactt atctggatat tgaccgtcca ataagtatca gcttgagtat ccccaatgac
     7201 ttgccggagt gtgagctggt gatagccacc agcaattccc cggaatattt gatttttgcg
     7261 gatgatctgc agtcgggcgc ggtggtttgt gatgttgccc gtccatcgga tgttgcccct
     7321 tgcgtttatg agcgtgatga tgtcttgatc ttggaaggcg gattggtcca atatccggat
     7381 cagataagtt ttggtcctaa tctgggttat cgcgacggcg tcagtctggc ctgtctgacc
     7441 gaaactgttc ttttggcctt ggaaggcgaa tgccgtgatt ttagcattgg caacaaactg
     7501 tctctggaaa cggtgcattt tttacgaaat attgcccgcc agcacggctt tgctttggca
     7561 ggattaaaga tgggcaatca cgaaataaat gatgaagcga ttgaaaagat ttatcatcat
     7621 tccctgcagc taaaaagagc cgggaattta taaggaggtt gccatggaat accaggaaat
     7681 aaagattacc cgtgagggca aatggttttt tggggaagcg gaaatgttca gacgcaatat
     7741 tttgcagatt ctggccaaaa atatcaatcg ggatgacaac gggtgctatt tcatcgaaat
     7801 gggatccgaa cggaacccgc tgatcgttga agatgtgcct tttttggcag ttggcatcgt
     7861 ggaagaagac gagcagccag tcaaactggt attccacgat ctgcaggaaa tggttctgaa
     7921 ccatgaactc cgtttgtaca tcaaagaaga tgttccctat atcagttatc gttggccggc
     7981 tgatacccgg ctgagccggg gcgtctattg gaaactcagt gattattttg attttcggga
     8041 agacgaggtc tatattgttc cgcctgctgc ccgcccgtca tctatcccgc aataacgttc
     8101 agaaaaagaa aaagggagga ctggctgcaa gccgggtcct ccgtttttgt aaccaaactt
     8161 tacaaacggc tgatttccgc cggacgaaaa cggttttcgg gtacggccag ggctgtcttt
     8221 actgcctcca gcataatttc cctgtcatcc ggtaaacgtc cggaaacatt ccaataattg
     8281 gagacatgat cactgagcac ataactgccc tcacaatgaa gatggctgat tagcaagccg
     8341 atttcctgca gggcttcatg cgggctcaac aggttgaact ttccctgttt gtaatcatcg
     8401 taaagcgggg ttcccggaac cgggacaaaa gtccgcaaac ggatgaaatc cggagaaaaa
     8461 gccgacaaca cccgggcgct ttccagggca tgctcacgcc atctttcctt gccgccgatc
     8521 cccaccagat agtattcgct ggtatcaatc ccgacctgtt tcagtttcaa gccggccgca
     8581 atgatttcgg cggaagtggt gcctttgcag atcttttgca gggtgacatc atcgccgctt
     8641 tccatgccgg tatggacgcg gctgagtccg gcatctttca gctgctgcag ttcttccaga
     8701 cttttcttgt tgacaaagcg cgctgaaccg tagacagtaa tgcgctgcaa gtaaggaaac
     8761 aattgcttgg cataagagaa aatttccacc aattgagcgg ttttcatgat aatcgtgttg
     8821 ccgtcaggga aaaacaaaga ttctacatat tctccgtagt aatcacgcgc agaccgcaaa
     8881 tcctccttga tctcctcgac cggccgaatt ttaaaccttg attccttgta cattgtacag
     8941 aaggtgcatt tgttgtgcgg gcacccgatt gtagcctgga tcaagaggct gttggcttcg
     9001 ctggggggac ggtaaacagt tccttcgtaa cgcatgggaa tactcctttc atgaatatta
     9061 ctgcatgtaa taaatattgc ttggctggta aaaggttctg agcaaaattg cgttaaagcg
     9121 ttaaattatg ttgctaaatc ggtaaaatcg gtaaaattta tttgttattt ggacagaaaa
     9181 tagatatatt aaatataata tagcattact gtcgccgtga ttccacattt ttaagcaagg
     9241 atggggtgag gctgaccgat ggaagtaaaa gttttgatcg tggatgatga tgaacgggag
     9301 agaatcgttc agcgttatgt attggaaaac ataaaagatg ttgagatcgt cggagaagcc
     9361 gtcaacggcc tggatgcttt gttgctttgc caggaaaaga aaatcgattt ggttttactg
     9421 gacataaaaa tgtcagagat ggacggtttt gaaaccgcca ataaactggt ccagttgaag
     9481 aatcccccgc tgtttgcttt tatcaccatg aacaaagata tggcagttga ggcgtatgaa
     9541 ctgggagcga tcgactatat tgtcaaaccg attgagcaga accggatcga aaaaacgatt
     9601 tcccgctcga aggcccagat tgcccatcac cattatatcg acgaattggt gaatcacaaa
     9661 ttgcgggaac gcatcgaaga ggtgttaaat cgatataaaa accaggaaca atatgccaaa
     9721 aagctgcccg tgagggaaaa aggcaaaatt gtactgctga aacccgaaga tatcgttttt
     9781 tgtgagagcc agggcaagaa agtgttgatt agtaccgtag atgaaggcta tttaagcagt
     9841 tatacgctga atgaattgga aaatcgcctt gaccaaagtt atttttttcg ggcccatcag
     9901 gcatttatcg tgaatctcaa ttacattaag gaaattatta atttcgggga gggttcatac
     9961 ctgctgaata tggaaaactc ccaaaaaaat atcatattaa gcagatccag ggcaaaaaac
    10021 ctccgggaaa aactagggat cggctaagtg taaacgagac tggatttaaa ttttgcatgg
    10081 ggaaaaaatc attccgttta ggatgaaaaa caaaccgctc aggcaacatt attagcctgt
    10141 tgtcagtata ccagggcacc cgtgggggac gccaaaggtc atttcttaat accgttggag
    10201 gttaaaaacg attcgtaccg taaataggcc aatgattgaa taaactgctg caaattatcc
    10261 tcttatttca acaaaatata ataatataga gaaataaatc acaatttagt atatagttat
    10321 aaggggtaat aattcgttac taattgaaaa ggaggaaagg agttttatag ctttcgctgg
    10381 tctgttcatg gtaattcagt tgacccgggc gtttataggg gtggaactga gtagatggat
    10441 tgaggctggt tgaggaagag ttgtcgtgca agatggggac aagcgcattc aatcttgcaa
    10501 ctttgttgga taggaggaat ggaaatgatt ccattttcaa agctcgaaag atatgagggc
    10561 aacgaaaccc tgttcgatct ggttaccatg tcaatcgtat cctctttggt gggcgaacct
    10621 ctgcatattc atgctgaagg tttaagaggc accggaaaaa ccaccattat gagggctgct
    10681 cgggggatcg ttcccaatat caccaggatt aaaaactgtg tctataattg cgacccggaa
    10741 catccgcatt gtccgcatca tcgccatttg agtccggaag aaattgcggc gatcggagtg
    10801 gaagatgttc ccatgccgtt tttggagata tcccattcag ccaaggtcgg gaccattgcc
    10861 ggcagtattg atttggcccg cttgaccgat acggctcatc ccgaagcaca gttgctgccg
    10921 ggaattattc cgcaggcgca ccgcggcatt atttttattg atgaaatcaa tcgtctggcg
    10981 gatacttctc cagaaatcac ggacatttta ttggatgtga tgggcaacaa acctggtcat
    11041 ctgcaaattg aagaagctgg tttgccggtt gtcgatgtgg aagtcagtgt atcggtgtgg
    11101 gctgcctcca acccggacga agatcccgga ccgctggaag aaatcagaag acaactctcg
    11161 gatcgttttg acatggtttg ctacatgggg cggccaacgt cggttgatgt tttgtcccag
    11221 atgctgaaag aaaactctca ttcccagaag attaaaaaag gaaaagaaat gcatctcagc
    11281 gcccgggaaa aagatgatgt gtaccggcaa aacattataa aatgggccgg acagtacgaa
    11341 aaagctgatc tgcctgactt tctgcgtaat tatatcgccc ggctttatgt caagcataat
    11401 cttgaaagta tcagagccat tgaggccatg caacagggag caattttgta cagcacaatt
    11461 aaaaaccgtg accaagccat gatcagcgat gttacccata tgattccgca ggtgctgaaa
    11521 catcgcgtgg acggcgatac cctgatcaag atgatcaatg atgtggaagt cagaggatcc
    11581 cgggatggta tactgaactt taaaaataaa aagaataatc ctgagaaaaa gaaagaagat
    11641 actgaatact tcaatacggc gggacgtccg ctcaaggatc tcggccgcaa tgaattaatc
    11701 aatacggaaa agacgcttgc cccgaaagaa ggttaaacaa gaggaagtgt tcctatggac
    11761 agtggctccg gcaacataag cactgctact tcggtaggca attatttaaa tctttacctg
    11821 ggccagggtc agggcgtaag attgggtgac agcattgtgg ttagccgcaa ggctcacgct
    11881 cgtatgcctg aaaaagttat caaagggcag atcaaaatat taattaacaa ggacgccggc
    11941 aagacgtatc ttgattatgt ggatgccgat caggtggttc acattgatgt ctaccacaaa
    12001 gagggagaga tcgatccccg agatgtagcc aaaagcattt tctgggcgct ggacaaccat
    12061 ttgctggagg atgagccgca gctgaaatat gcagaagatg aaaggttcag aattgtcgcc
    12121 gactatgcca acaaagtctt tttaaccacc aacagcggca agggcttgct ctatgatctt
    12181 ttccaggaca aattgactcc ttacagcgaa ggcgaggaag gctttgatca tgtgcatatc
    12241 ctggcccgct ttaatcgcga tgaatatgcc ctggtctatg tgatcagcga agcggttgag
    12301 gcagcgattc tggcttcgga tgtcaaactg gccaaggtcc gtaacatctc tcatggcaag
    12361 gaaaagaaac aagaagaaaa ttttgccggc aacatgaagc tgccctggaa gaaattcaag
    12421 ggaaggatcg cttcccaatt gcccgacaag gaaaatgtca acgaactgat tatgaaactg
    12481 gccgagaaat tcggcggagt agaagaaatt gaagagtgga tggactgcta ttctaccaac
    12541 atctttaaac gcaaaggggt agaataccaa aaaaaaaagt ggggcgacgt ggaacattat
    12601 gtcgaacagc ttgaagaatt aggtctgatc gaaccggggc tcaccggacg caaacttagt
    12661 aaacgcggcg tttttttgca tgattatatc atcaagcaca aaatcgaact ggaaaccgaa
    12721 atcaggcgca atatgcgcaa ggtcccggga ggaggagcca atcgcttccg taaatacggc
    12781 aaaagtgaga gcaaacattc ccaggtggaa ttcaccaatt acaaccgtac ggtcaacaat
    12841 tcggagaaga attggtccgg caacctggcg gtaccggaaa ccgttattca ggccaagaag
    12901 aataattttc tgcgcggcga aaagcatttt acgctgcaaa aagaagatct gcatttttat
    12961 gataaaaaaa cctatgttcc cattgatatc tgcctgctga ttgatgccag cggcagtatg
    13021 gccggggaga aacggcaggc cgcttgtttc ctggcagagc atttgttgtt gagcggcaag
    13081 gaaaaaattg ccgtggtcac cttccaggag cattccagtg agattgttgt tccctttacc
    13141 cgcaatcaga aacagttgag caaagggctg gcgaccatca atccggcggg cttgacgcct
    13201 ctggccagtg ggattatgac tgctttgaag ctaatcaaat cgacgcgact gcgcaatccc
    13261 ttgctggtat taatcaccga cggcattccc aatgttccct tgtggaccat ggatgccaaa
    13321 acagatgcca tcaccgctgc cgctcaatta aaagacacca aggttcgttt tatttgtatt
    13381 ggcgttgaat caaaccggat ctatctggaa aagctggcta ataaagccgg cggatccctc
    13441 tatctggtgg atgatttaaa caaggaaaat ttgatcaata ttgtgcgcaa tgaaaagaag
    13501 gccatcagca cctcggaatt gcaaagctga tacgggggca gtgcttgtcc ggccatcata
    13561 taaacaggat gtccaactca cccgggagac gcgatgattg cgttgccgga gtgggtttta
    13621 tttttcataa ggatgcataa tttaaattgc cttcccgtaa tcttatagca gcaatatata
    13681 aggggagggt ttaagatgcg caacacaatg atcgtggttt tggcaatgat tgtgctggga
    13741 atttgtctgc tgagcggagg atgcagcaga ccggaccaga gtgtaatcag tgaatggcag
    13801 gagttgttaa aatttccggc gggtgatcag caagccgcaa aaggcgggaa caatcccgat
    13861 atcgctcctt taattgaaac cggtacgggc atgaccgggg agacgattac cgtgagcctg
    13921 tattttgccg atgccaccag acagcaattg gtacgggagg atcgcgccat tgccaaagtg
    13981 gaagggattg cccgggagac ggtggaagaa ctgcttgcgg gaccggccaa tgcagagtca
    14041 acggaggttt ttccggctgg caccaggctt ctggatatca atctgaaacc ggacggttta
    14101 tgtatcgttg atctgagttc tgaagcctgt caggtaacgg atcgatccca ggcacaactg
    14161 atggtggaag ccatcgccaa aaccctggga cagtttcctg ccgtacagga agtcaccttc
    14221 atgattaatg gagaaaaaat agacagtata ggcggttatg tggatctttc cgccccgatc
    14281 ggcactgatt accaggcatg agacgggtcc tgcagaagaa ccgcaggcaa tactggtaca
    14341 cagcgtataa aagtgataaa gtaattatga attggattgg aagtccaggt tgaactgggg
    14401 gctgagtcca accgtcttca ataataagtc gcaaattaat tagaggtgtt gcattttgaa
    14461 tgataccaga ccgattggta ttttcgattc aggcgtcgga ggcttgacgg tagtaagatc
    14521 cctattgacg cagcttcccg gggaaaattt catctatttc ggtgatacag ctcatctgcc
    14581 ctacggcaat aaaagccagg ctgagctgtt tcaatatgcg caggatattg tggggttttt
    14641 actgcagcag caggtaaagg cgattgtagt tgcctgtggg acccattcat cgattaccct
    14701 gcccgagatg gaagatcgtt gtccagtgcc cattttaggc gtggtcaagc ccggggctgc
    14761 cgccgccgtc gccgccaccc gcaatggcag gatcggcatc attgccaccc aggcgagtgt
    14821 caatcagggt tcctaccggc agcagatcgt ctcccaaggg gagtaccagg tttttgccag
    14881 tgcctgtccg cgctttgtgc ccttggtgga agccggacat tttgacggtg ctgaagtaga
    14941 ggccgccgtt gccgaatacc tgtctccttt gctcgcaaac gagatcgata cactggtcat
    15001 gggatgtacc cattatccct tcctggcaga agccattgcc caatatgcag gtccgggcgt
    15061 gacgctggtc gatccggcca tcgatacgat taaaacactg gccgcggtat tgcaccgtga
    15121 ccatctgttg ggatcagcag ccctggggca gggtcggttt tttgtgagcg gcaatgacca
    15181 gtctttcttt cacgccgggt ccatgctgtt acagcatcaa ctcatccaaa aggttgagaa
    15241 aatcagtttt gattaaatca gggggacatg atgaatcata ccattggctt tacaaccagt
    15301 atcccggttg agattattct tgctgccggg agggtccctg ttgatctcaa caatatcttt
    15361 atcacttccg ggcaagctgc gcaatgggtg gaaaaggcgg aagaagcagg ctatccgcgc
    15421 aacagctgcg gctggatcaa aggcctttat accgccggtc tgctgactca accggaagca
    15481 gtcatcgccg tgatgcacgg agattgcagc aatacccagg cgctgatgga gaccatgcag
    15541 ctgcggggaa taccgatctt tccctttgct tatccgtatg accgcagtgc tgacatgttg
    15601 acgctggaaa tggaaaagct gatgcgggtt ttccaggtca gctggacaca ggtggaggat
    15661 gtccgccggg atctgctgcc gctgcggcaa ctgtgtcagg aattggaccg tctgagttgg
    15721 caggaaaacg tgctgactgg agcggaaaat cattactatc tggtgtgttc cagtgacttt
    15781 aacggagatc cgcagaagta tgcccgggac ctggaagaat ttattgctcg ggctcaactg
    15841 cgccggcctt ttcaggacga agtcaggctg gcttatatcg gggtgccgcc gatattcacc
    15901 aatctgtatg aatacgttga atcattgcag gcccgggtcg tatataatga gattcaacgc
    15961 cagttttcca tgcccttcgc ctgtcaggac atcattgatc agtatttgca atacacgtat
    16021 ccttatggag cgtttgcgcg gctggaagat attcaggaac aaatccacct gcgtcaggta
    16081 gacgggatta ttcactatac ccagacattt tgtttccgcc agatcgagga catggtgttt
    16141 cgggaaaagc ttgatctgcc gattttgacc ctggaagggg acaagcccgg tccgttggat
    16201 gcgcgcagca agatgcgcat cgaggccttc atcaacatgc tgaaatttta aattaacagt
    16261 atcatcagat aatggtcgga gagtgaaacc atgctgggaa tggatctggg ttcgcgcaac
    16321 gtgaaaataa tggaaatgca ggatcatgtc atcactgcgc agctgagctt tgatacgatc
    16381 gcgttttatc gttcctacgg caaaaccggc gcaggcaacc tgcgcattga tttgtcggaa
    16441 ttgggtttgg cccaaaatca acccctggtt gcgaccggtt acggcaaggt cagtgtgcag
    16501 atcgaagggg ccctccatct gcctgaaatc caggcacatg tcaaaggggc tgtttatcag
    16561 accggctatc aggattttac gttgctggat attggcggac aggacactaa aattgtgaag
    16621 gtgcgcggcg gccgggcggt ggattttctc accaatgacc gctgtgcggc cagctcggga
    16681 cgttatctgg aaaacatggc tggtgtgttg ggcattgatt tggaggaact ctcccgctac
    16741 agtctccaac cggtggaact gaattcaacc tgtgccatct tcggggaaac ggaaatcatc
    16801 ggcaagattg tggaaggcta tcccctcgca gaactggcgg ccgggatcaa ctattcactt
    16861 tatcgccgtt ttgccgggat gctggagaga ctggccagcg acctcatcgt agtatccgga
    16921 ggggtggcca ggaatgaagc aatagtcaac attatcgccc gggaaagcga ccgccaggtg
    16981 atgtgtttgc ccgaggcgca gtttaacggg gcgctgggtg cttgtattta cggatatgaa
    17041 aaattttcaa agtgacataa aggagaaaac atgcggcaga taaatgtgga cacaatcaga
    17101 caggcagtca aggaaatggt gatggaagcc aataccagac tgccgggaga tctgcaggca
    17161 gcgctggtat ccgccaagga aaaagaaagc tcctcccgcg gccgccagat cctggagatc
    17221 tgcctggaga atgctgccct ggcagcgtca gaaggcatgg cactgtgtca ggacaccggc
    17281 ctggtggtga ttgatctgga aatcggtcag gcattgcagc tgctcggggg cgatctggaa
    17341 aaggccgtta accaaggggt cagagagggt tacctggccg gcagcctgcg caaatcgatg
    17401 gtcagcgatc cgcttcaccg ggttaatacc ggtgataata caccagccgt catacatatt
    17461 cacatcgtac cgggagaaaa cttaaaaata acggttttgc ccaaaggagc aggcagtgaa
    17521 aacatgggac agatggccat gctgaagccg gcccagggtg tggagggagt caaggaattt
    17581 gttttcagag tcgtccggga agcgggcggc aatccctgtc ctcccatcat cgtgggcgta
    17641 ggcatcggcg gtaacatgga aaaggtggcc ttgctggcca agcaggcatt aatgcgggaa
    17701 atcggacagg tccatcccga tgcacggctg gccgatctgg aggcagaact gctgacagca
    17761 atcaatcaaa tgaatattgg gcctcagggt ttggggggcg acacaacggc gctggcagtg
    17821 catatcgaaa cctatcccac ccatatcgcc tgtctgcccg tagccgtatg cattggatgc
    17881 cattccaccc gtcgtttgag caggattctg taaaaaggag agggaagaat gacgaaaatt
    17941 ctatcagccc cgctagcccg ggaagagatt gaagcattgc agaccgggga tgaagtgctg
    18001 gttcagggaa ctgtttatgc cgcccgggat gctgcccatc aaaaaatgac ccaggcattg
    18061 gccaatggcc agggcctgcc ggttgacctg caaggacaga ttatatacta tgtcggaccg
    18121 tgtccagccc cggaaggacg ggtgatcggt tcggcagggc cgaccacctc cggaagaatg
    18181 gacgtgtata cgccgctgtt gctggaacag ggactcaagg ccatgattgg caaaggtccc
    18241 cgcaatgaag aagtgatcgc cgccatgcag gcgcaggggg cggtttatct ggccgcagtg
    18301 ggtggcgccg gagcttattt ggcccaacgc attattgctg cccaagtggt agcctacccg
    18361 gaactgggac cggaagcgct gcttaaattg gagttcaagg acttcccatg tattgttgcc
    18421 attgattgtc gaggcaataa tatttatgcc ggaaatagag aagaaggagc atctgtatga
    18481 gcagacctga agaacggaaa aatgatgaat tgcgtccggt gcgaatcacc ccgggatttg
    18541 taaaaaaccc ggacggttca gttctcgtcc agaccggcaa taccatggta ctgtgtacag
    18601 ccatggtgga ggaaaaggtt cccccttttt taaaaggcac cggaaccggc tgggttacgg
    18661 ctgaatactc gcttctgccc gcttccaccg atacccgcag tccacgtgaa gccggcaaag
    18721 gcaagattac cggacggacc tcggagatcc aaagattgat tggccgctcg ttgcgggcgg
    18781 tagtcgattt tcaagccttg ggagagcgga ccatctggct ggactgtgac gtactgcagg
    18841 ctgacggagg gacaagaacc gcttccataa ccggcagttt tgtggccttg tctctggccg
    18901 tggaaaaatt gctggccaag gggacgctga cgcagaaccc gattgtcgat tacctggccg
    18961 ccaccagcgt gggcattgtc aacggagagg cgctgttgga tctggagtac gtagaagatt
    19021 ccgctgccga agtagatatg aatgtagtga tgaccggtcg gggagagttt gtggagatcc
    19081 agggtacggc ggaaggaaaa ccattcagcc gtcaggcaat ggatgagctg ctgcaactgg
    19141 cggaaaaagg aatcaacgaa ctgatcagcc tgcaaaaaaa gattctcacc ggggagcagt
    19201 agacagtcat gaagacgaaa cttctaattg ccagccgcaa ccaaaagaaa aaaaatgaac
    19261 tgcaacaaat tctgagtgat tcggcatttg ccttgctcag tctggatgat ctgccgccgc
    19321 tgcctgaagt agaggaagac ggcgatacgt ttgccgaaaa tgccatcaag aaagcggtaa
    19381 cagtggcccg acaaaccgga ctgctgaccc tggccgatga ttccggtctg gtagtggatg
    19441 ccctgcaggg agaaccggga atttattcgg cacgttatgc cgggccggag gccaatgatg
    19501 agaaaaacaa tcaaaagttg ctgttggctt tgcaggatac gcctgagcaa gaacgtaccg
    19561 cccgcttcgt ctgtgtgatc gccctggcgt ttcccgatgg ccgggttata accgtggaag
    19621 gcacctgtaa cggcaggatt gaccgacaat ccagaggcag tgaaggcttc ggctatgatc
    19681 ccctgttcgt gccggacggg tatgaagaga gttttgccca attgtcagcc caggtcaagc
    19741 attccatcag tcatcgcggc caggcgctgg agctgatcaa accttatttg cagcggatta
    19801 acctggaggg ataaaatgct gattgcagtc gtggctgata cccacggtga aacagacggc
    19861 gtttgccaac atttaaaaca atggaaaccg gatcggctga tatttgccgg agattattac
    19921 cgggatggag agaagattgc acaggctttg caaatcccct atcacggagt caggggcaat
    19981 tgcgaccgcc atgatccccg cgcccgggat gaagaaatca ttgagatcga gaagaaaaaa
    20041 ttctatgtcc tgcacggtca tctctatggt gtcaaacaaa gtatcagccg gctgtattat
    20101 cgggccttgg aaatcgaagc cgaggttgtg atttatggtc atacccatga cgcccacctt
    20161 gaacaaattg attcgctgtg gatgattaat cccggcagtg ccagccgccc tagatttgac
    20221 agtcgtggca gctatgtatt gatcgacttg cagtcagagt ctcttttgcc tcgattggtg
    20281 aaaatgtaaa aaatattttc aaaaatgatg gtaacttttt tatagatgcg ggatgataat
    20341 ggtatgggca attcatgccc ggcttaatca aaggaggatg acacgttgaa acaaaccgct
    20401 ctcatatcaa tgcaccgcaa atatcagggc aaattaattg attttggagg atgggaactt
    20461 cccgtacagt atgcaggcat tctcaaggaa caccacatgg tcaggcagaa ggcaggtctt
    20521 tttgatgttt cccacatggg ggagatcgaa gtcaaaggtg acggggccga agatttcgtt
    20581 cagaaactgg tcaccaacaa cgtcaaaatg atggccgacg gcgaagtcat ctatactccg
    20641 atgtgctatc cccatggagg cgtggtcgat gaccttttgg tctaccgcta ttctccccaa
    20701 cattatttcc tggtggtcaa tgctgccaat accgacaagg attatgactg gattaaaagc
    20761 aactgcccgt ccagccttgc ttgcagcaat gtctccagcc aatatgccca actggcggtg
    20821 caaggtccgc aagcccagga gatcctgcag aagctaacga atgatgatct ggagcagatc
    20881 gagtttttcc attttgtgcc ggcaatgcag atcgccggga tttcctgcct gatttcccgg
    20941 accggatata ccggtgagga tggctttgaa ttgtacgttg ctcccggaca ggctgaacaa
    21001 ctatgggaag ccgtactgca agccggcggc gaggagattg ctcccatcgg attgggggcg
    21061 cgtgatacgc tgcgatttga agccaaactg ccgctttacg gacaggagat tgatcaggat
    21121 atcaatccgc tggaagccaa actggggtat tttgtgaaat tggacaacga tgattttatt
    21181 ggccgtgatg tcttgcgcca agtaaaagaa aataaaccgg cccgcaccca ggttgagttt
    21241 gtcatggtgg gcaaaggcat tccccgttcc cattatgaag tgcaaaaaga cggacaaaaa
    21301 attggctggg tcaccagcgg gggttattct cccacgcttg acaaaaatat cggtctggcg
    21361 ctcatcgaca atcgcggtta taaaaacggc gaacagatcg atattataat ccgggataaa
    21421 cctgttgcgg cacagattgg cgagggactc ttttatcgga aaaaaaccaa acccaaaagc
    21481 aaagcgtaag cagctggtcc tgaaaagcaa aaaacaagga ggaagatcac gatgaacatt
    21541 ccggaaaatc tgtattattc aaacgaacat gaatgggtac gcgtggaagg cgaaaatgct
    21601 tatatcggca tcactgattt tgcccaggaa catttgggtg acatcgtatt tgttgaactg
    21661 cctgagccgg acacggatgt ttccgccggc gaacaagttg gcgtcattga gtcggtcaaa
    21721 gcagtttcat ccatgttcag cccggtcagc ggtaccattg cagagattaa tgaagcgctg
    21781 gaagataccc cggaattatt aaatgaagac ccttatcaaa atcatatcct ggttgtcaga
    21841 atgagcgatc cggacgacct ggatgcgctg cttaccccgg taaaatatgc ccagctctgt
    21901 gaagaagagg agcgaggagg caaataacat ggcctataca ccaaattcac agcgggttgc
    21961 cgaggaaatg ctgcacagca tcggcctgga aagcatggat gaattatttg ctgatatccc
    22021 ccgcgaagtc aaattggatc agctcttaaa cctggcgccc ggtctcggcg agatcgagct
    22081 gcgtcaatac atgcagacac tggccggccg caacctcaat gttgatcagt acccctgctt
    22141 tttgggggcg ggtgcttatg atcattttgt tccggccgtg gtggaccaga tcctgcagcg
    22201 ttcggagttt tatacttcct acacgcccta tcagccggaa ataagccagg gaattttaca
    22261 ggccatattc gaatatcaga ccctgatctg tcagttaacc ggcatggaag tctccaatgc
    22321 ctccatgtat gatgccggta cggcattggc ggaagcctgc ctgatggcct gtgaggccag
    22381 taaaagaaag cagattctgg tgccggaaac gatccatcct gagtatcaaa aaattatgga
    22441 tacctacagc atttccggca aaatgaactg ccaggcggta ccgggacgca acggagttac
    22501 ggatgttgaa caaatcctgg ccctgattga caaggagaca gcggcggtgg ttctccaaac
    22561 gcccaacttt ttcggcaatc tggaggaaat tgaaagaatt gaacaggcca tccatcagca
    22621 aaagggattg ctgatcatgg tggttgatcc gatttctctg gggatattaa aatcgccggg
    22681 tgagtacagg gcagatattg tggtgggcga aggacaagcg ctgggcaacg gtttgaattt
    22741 tgggggacca tacctgggat ttatggccag cagcaagaaa tatatgcgca agctgcctgg
    22801 tcgaattgtg gggcagacgc ttgattttaa aggccgcaga gcctttgtgc tgaccttgca
    22861 ggcgcgcgag cagcatatcc gccgcgagca agcttcctcc aatatttgtt ccaatcaggc
    22921 gttaaatgcc ctggcggcca gcatttacct ttccatggtc ggggcgcagg gacttcggat
    22981 gattgcggag cgctgtcatc aactggccgt ttatgccagc aatgaatttg ctaaaatcgg
    23041 tttgtcgttg caatatgacc agcctttctt caaggaattt gccctaaagg tcaaggagcc
    23101 cggtcgcgtg aaccgtctgt tgctggacaa tggcatcatc ggcggctacg aacttgaaga
    23161 tgcgctcttg ctggctttta cggaaaaacg cagccggatg gaaattgacc gcctggttgc
    23221 tctgatcgga ggggttgaac atgaataaaa ttctatttga aaagaatcaa cccggcagca
    23281 atgggttctc gttgcctccg cttgattttc cagcagatgc attgtcctcc accataccgg
    23341 cagcgtttct gcgtcaggcc gagcttgatc tgccggaatt aagtgaagtg gaagtggtcc
    23401 gtcattttac tgctttgtca catcgggcct acggggtgga tgacggcttt tatccattgg
    23461 gttcctgtac aatgaaatac aaccccaaga ttaatgaatg ggcggcacgc ctgcccggac
    23521 tggcggccat ccatccttat caggcgcaag agacggtgca aggcgccctg gagatactat
    23581 acaaggccga taaagccttc agtgaaatcg ccggcatgga tcgctttact ctgcagcccg
    23641 cggccggggc gcatggagag ctgaccggcg taatgatcat caaagcctat caccgccatc
    23701 acggcgacga acagcgtacc cgcatgctgg tgcctgactc cgcccatggc accaatcctg
    23761 ccaccgccaa catggtcgga tacgaagtgg tggagatcaa atccaatgat ctgggactgg
    23821 tggatctgga cgatctgcgc agcaaaatga accgggaaac ggccggactg atgctgacca
    23881 atcccaatac attaggcctt tttgaacagg atatccaggc cattgccgaa atcgtgcatg
    23941 aacagggcgg cttgttgtat tatgacggtg ccaatctcaa tgccattgcc ggggtcgcgc
    24001 gtccgggcga tatggggttt gacgtcgtcc attttaacct gcacaaaacc ttttccacgc
    24061 cccatggcgg aggaggacca ggttccggac cagtgggtgt aaaagagcac ttgagcgcct
    24121 tcctgccggc tcccctggtg gaatgccagg acggcaggta ttggcttgat gatgaccggc
    24181 ctttgtcgat cggtcgggtg aaagaattct acggaaattt tagtgtaatc gtcaaagcat
    24241 attgctatct tcttaccctg ggggcagcag ggctgcgttc agccagcgaa catgccgtgc
    24301 tgaatgccaa ctatctgcgc caccatctgg ccagtgacta ccaaattccc ttcaatcgtt
    24361 tgtgcaaaca cgaatttgtc gctaccagtc aaaatcaaat ggaagcggcg catatttcaa
    24421 ctcttgatat tgccaagcgc ctgatggatt acggttacca tcctcccacg gtctacttcc
    24481 cgctgattgt caaagaagcg atgatgatcg aacctacgga aacggaaagc cgtgagagac
    24541 tggatgaatt tattgaagct ctgcgcagca ttgctcgaga agcccgggag aatccggaga
    24601 tggtcaaaaa tgctccccac aacaccgtga tcgtaagggt ggacgaagtt gccgctgcgc
    24661 gtcatccatt ggtcaaatgg gaggcagaag cgtgaaggat cttttgatca ttggcggggg
    24721 accgggtggc tatgtagccg caatccgtgc ccgtcaactg gggatggagg tcagcctgtt
    24781 cgaacacgac aaactgggcg gaacctgcct gaatcgaggt tgcatcccca ccaaggctta
    24841 ctataaaaac gctgaagttc tgcggaccat gcagcacctg agcgaatata gcatccgctg
    24901 catggatgtg cagttcaatc tggcgctggc taaagaacgc aaggacggga ttgtcaacaa
    24961 gctggtgacc ggcgtggaag ggctgctgca ggccaacggg gttgaagtca tcaccggcca
    25021 tgccgaaatc atcgctccat ccgctgttcg ggccaacgga aaagaatata ccggccagag
    25081 aattttgatc gccagcggct ctgaagctgc gcgcccgccc cttcccggta tcgacctgcc
    25141 cggtgtggtg accagcacag aactgctcga gcgggaagag atgccggcgc aaatggtcat
    25201 tatcggtgcc ggggtggtag gcctggaact ggcttgtata ttcaactcct ttgggagccg
    25261 cgtaaccgtt ctcgaggcag cagatcatat cctgggaacg ctggacagcg aactggtcaa
    25321 aagaatgaat gtgtttttaa aaagacaggg gatcgacctt catacctcct gccggattga
    25381 ggagattcag tcagggcagg atcaattgat ggtccttgcg caaggcaaac gagaccgtgt
    25441 acgggtcgag gcagatctgg tactggtcgc cgccggcaga aaagccgcca cccaggggtt
    25501 gtatctggat gagctgggca ttgtcaatga gcgagggttt attcgggttg acgagaactt
    25561 tgagagcagt gtcaaaggga tttatgccat cggggatgtg attggcgggc agatgctggc
    25621 ccacgttgct tcggaggaag ccgtggtggc agtcgagcgc atgtccggca ttgacagtca
    25681 ggtggcctat catgccgtcc ctgcctgtat ttttaccttt ccggaaatcg ctgcggtggg
    25741 catgagcgag gagcaagcca aacaagccga aattgcttat aaagtgggta aatttcaatt
    25801 tgcagccaac ggcaaagcca tgaccatgag tgaaaccgat ggtctggtca aagtcatcgc
    25861 ggatcaggaa gaacggatca ttggggtcca cattctgggt ccgcatgctt ctgatttgat
    25921 cctggaggct acgctgatgg tcaagaacca catgcagtgg gaagaaatcc gcgggaccat
    25981 ccacccccat ccaaccttgg gagagagtct ggccgaggca attcttgacc ttaaaggaga
    26041 agcaattcat ctgctgccgc gccgcaaata agctggcgaa agcgatgtaa aatggtttta
    26101 atcaaggcgg gaatgactcc cgccttttgt tataatgaaa gacaaggtca acacgtccga
    26161 atcaacagga aaatttaggc tgtatcggca gtgtttcata agtattttcg gcggaggtaa
    26221 agatgataca tataataaat ccttccaccg acccgtattt taatctggcg ttggaagagt
    26281 atctgctgct cgacccggat catcaagagg agtatttcat gctctggcag gatcgcccgg
    26341 tcgtggtggt gggacgcaat cagaataccc tgaaagaaat tgatccggct gcggttaaaa
    26401 gcaggggggt cgaggtggtg cgcaggctga ccggaggggg agccgtctat cacgatttgg
    26461 gcaatttgaa ttttacttat atcatcaatg acgaacaaca tatcggcttt gattttgcac
    26521 gatttaccgt accgattata acaacccttg cgcagttggg cattcaggcg gaaaacagcg
    26581 ggcgcaacga catcaccatc aaggggaaga agttctccgg caacgcgcaa taccgttgga
    26641 aaaaccgctt gctccatcac ggcaccttac tgtttgacag caatatcgaa gccatgacca
    26701 cggttttaaa taccgatccg gccaaactcg tatccaaggg agtggattcg gtgcgcagcc
    26761 gggtcaccaa tattcgggag catttgccca acgagatgac gctgccgcaa tttcgatccg
    26821 ccttgctgac tgcctgcaag gccatgggat tgctggaggc ggaacaggcg ttgcaggaag
    26881 aggaacgaca ggcggttgcc gggctgcaac gggaaaaata ccagacctgg gaatgggtct
    26941 atggcagttc gccggccttc aacgttcacc accaagcggc ctttgactgg ggcaaggtgg
    27001 agattggcct gtgcattgaa aacggtttga tcagcgactg caggatttat ggagatttct
    27061 tctcgctggc ggatgtcaat caattatgtg cagggctgca gggggtgcct taccgggcag
    27121 agacgctgca cagaaaatgg gctgagcttg attggcaatc ctgttttccg tctgcccaga
    27181 cggatgaact caaagcgatg tttttaaaag atgttccctg atgatcgcat atataaaggg
    27241 aaaattgagg ggatgaaccg atgcaattac agcatatcac cggcaacagc tattttctgg
    27301 agggagacaa ttgcataggc gtatatgtgt ttgcggaccg gagttgtctt ttggttgaca
    27361 gcggcgccag tgagagccag gcaaagaaga ttctgcactg cctcaccgaa gcaggtctgc
    27421 aaatcgatac gatttttacg acccatgccc atgttgacca ttgcggcggc aaccaatact
    27481 tgcagtccca gtgccaatgc cgtgttatgg cctcgtctct ggcggcagcg attttggaaa
    27541 acccgatgct gggaccggcc atgctgttta ccgcctatcc gctgcgggtt ctgcgcagtc
    27601 gggtgttgat ggccgccccc tgcaaggtca atcagaccgt cagcccgggc cctttgctga
    27661 ttcaaaacga actgtttcag gtgtatgatc tgcctggtca cagcctggga caaaccggtt
    27721 tggctacgcc cgacggggtt ttatttctgg gagacagcct gatgcatacg cagatgctgc
    27781 aggaatatcc gtttttatat atggtcgata tcacccggca aagggagacc ctggccttca
    27841 ttggcagcca tcaatggcct tgggttgtgc tgtcccatgg aggattggtg gaagatttgg
    27901 acgattgcgt cggacaaaat caagccagga ttgctattat tgccgaatca attctggcct
    27961 tgctggatca gggccggtcg catgaagaaa tcctcgccgc cctgctgcgg caatatgagc
    28021 tgagtgccaa cagcggccag tattatctgg tttcctcgac gattgccgct tttatttcgt
    28081 atctcagtga acaaaagctg atcaggaacc gcgtggagca aggggttatg aagttttacc
    28141 gcatttccct gcgtgatcgc caggaggaat aaacggtcac ggtatttcgg gaaattggca
    28201 attatctaca gaataactgg ttgaataaaa aagggaaaca gcagcagaac cagacttaac
    28261 aagatgaata cggtggttat tgtccagcgt atgtttttat tgggaatcca tttgcggtat
    28321 atggccacaa cctgttgcca atgcaggtag atgtgccaga aaagcaaaaa caacaggaaa
    28381 taacccagat aaagatggat aactgcccaa ccatcccggt cgatgcccag caacaacatg
    28441 tcaaccttgc cgccaaaggc tgctttggcg gcggagccgc tcaacaagat gtacttgcgg
    28501 acataccccg tacccaccag ggccatcatt cccaacaaca taatggcatc aagaagcaaa
    28561 ttgattcgcg atttgttcat ttttataacc attcctttct tgaggcataa aacgtcatga
    28621 aagcctttct ttctgacttc aacctctttt tttcacaaca gacaaccggg ctatgcttgt
    28681 gcagcgcttg tcaggatacc taccatgctg caatgatctc tgtcaaacgc cgaaaagcat
    28741 cctgcaggtt ttcctggctt aattcccaaa ccgtgcgccc atccagcggc aaatcatgaa
    28801 ttcgctcaca atggggaaga ggatccgcga tccgttcctc cccgaaatga tcggctaaaa
    28861 gggaaaaaca ctccaggttg ttgtgccact ccttggtatt gattttgttc atcaccacca
    28921 gatcaatcgg ttggggacgg ccggtcaggc tggacaaatg ctgatcaagg tcgacaacct
    28981 gacgggccgt atctttttct tccagactgc agcagaccag gatacgatcg cacctgggca
    29041 gcagattttc gcatccagca gaggtggcag gcagcagaaa aacatattcg tagtcaactg
    29101 caatttcggg cagaatccgt tctgcgtctt tcatcatcca aaaatcggtt ccaatacggg
    29161 ttgctacggt accgggcatc atttgtcctg atgatggcat tgggcggtca gctgcccggt
    29221 caaatcccag ccaggctctc agtttcttgt tttctccctt ttctgcgatg agcacccggt
    29281 gtccctgacg ggccagagca ctggcaagat ttacggtgat cgtcgtttgc cctgcgtttg
    29341 cgtgggccga cagcacgagg atgattccag ccaaataatc acctcccgga aaatacttat
    29401 agaaacatca taacagggaa ttctatgtaa agaaaaacat tcgcagcgat taagcttatt
    29461 ttatcaggta aagaatcaaa tctaaacaag catatgttca ttattttcaa aaacgttgca
    29521 catggggtgt catttatgtt attaataaaa cataacaaaa agttatacag ggaaaggagg
    29581 ccagcaaatg cccgaagaat tcaaaccagg ctgtcaacgg ccgccaatta aaaaggtcaa
    29641 tgtacaaaca gaagaaatcg caacaacaga actgattacg aaggaggcga agcaaagcat
    29701 ggttaaagta ggaaaaccgg ccccggattt taccgccgct gcctttgggc aaggcaagtt
    29761 tgtgaatacc agtctttcag agtataaagg aaaatgggtg ttgctttgct tctacccggg
    29821 tgattttacc ttcgtttgag cgacagaagt ttcagcagtt gctgaaaaac atgccgaact
    29881 ccaggagctg ggagttgaag tactggctgt aagtgtagac agtgtttttg tccacaagat
    29941 gtggaatgat cacgaacttt ccaaaatgat caataaagat atcccgtttt tgatgtgctc
    30001 cgaccaggat ggcagcatcg ggaaaatgta tggcatttat gatgaggaca gcggcactga
    30061 aacccgtggt cggttcatta tcgatcccga tggcatcatt cagggctttg aagtactgac
    30121 cccgccagtg ggcagaaatg tttctgaatc cattcgccaa gtaaaagcct tccaactggt
    30181 gcgtgcctct caaggcacgg aagccacgcc ttccggctgg aaacccggca aaaccacttt
    30241 gaaacccggt ccggacctgg taggtaacgt ttggaaagta tggaaagtca gcgaggcttt
    30301 tgaagactag atagcaccca aacacatgcg taatcagcaa gcaagggctg tttctgatat
    30361 cttcagaaca gcccttgtgt ttctggtttc tgcaatcttg ctggatagaa tgcataataa
    30421 ttttatgaag gatgttcgcg ggaatgaaag caaaaccaaa ccctacaatc attcaggaaa
    30481 atacactcca ggtaatggca gatacattaa cgagaatcga cagtctgaag tgttttcata
    30541 ttcccattgt tttaagtgtg attgaacaat ataaagttgc tggcattgaa ggctatttca
    30601 tggaacagca ggaattgcag ctgcaaaaac tgctgtcgat gctggttcaa ttggaagaaa
    30661 agcttgcccg gctgcagcaa cgtttgctta tctcttgagg gaatatcccg agaattcaaa
    30721 cgagagataa tactgcggat ataatcagtc aaaagatcgc tcccaagcgg ctttaggtat
    30781 aaccctcaga caaagctaac aatagttcag atgaaaaagt ttaaaattat caaaaatcgg
    30841 ggctctcagc cccgattttt gctggacatg gcgttattcg tcaaggtgag tccgccgaaa
    30901 gtaaatcatt tgttgacgat ttcggttgtt aattgatgat tgacaggaca ggctgacagc
    30961 gcaataatag taaatgcaac ttcttgcaat tagaaaagga gtggtttgca gtgaaaaaat
    31021 ccttgcaaag aataaagcgt ttgctcgcgt tgatggtact ggcattggtt ttgctgccga
    31081 tcgccggatg cggcaatcct gccccggaga ccgtgtcatc cggcgcagga aaaatcaaag
    31141 tagcggtatc gatcgtgcca caggcaagct ttgtcgctgc tgttggcggt gatctggtgg
    31201 aaatcgtaac catgattcca cccggcggca atccggaaaa ctatgcaccc aacccaagaa
    31261 tcatggagca ggcgagtgat gcccggatat actttgccat cggggttgca gccgaacaaa
    31321 acggcatttt gccaagactc aaacagctta gtgcggacat gtcggtgatt gatttggcca
    31381 gccgggccga tgcagtctat ccagctttgg aaattgttcc gggcgaaaaa gatccgcacc
    31441 gctggatgtc tccacgtcgg gtcagcatta tggtagagat gattgccgat gaattggccc
    31501 aggcagaccc ggaacacgcc aagatttacc agggaaatat gcaaaactat caggcgcagc
    31561 tggaaaaact gcaccaggag atcgaagcct ccctggcgga cgcagaccac cgcagtttta
    31621 tcgtttatca tccttccatg ggatattttg cagatgatta cggactcacc atgattgcct
    31681 tggaggaaga agggaaagca gctactgccc gccatattca gcaggtgatt gaccaggccc
    31741 gacaggaaaa catcaaagcg atcttctatc aggcggaaat ggatggcaaa caggcccaga
    31801 ccttggcccg tgaactgggc ggggaggcgc gcctgatcgc tccgttggca cctgattata
    31861 ttgataacct gcgccaaacg gcgcagacat tcagccatgt attaaagacg gaataaaaac
    31921 ccggcaggca tcatgaatta gccgacattt aagtgacact ggctggacgg gaacgcataa
    31981 aggtgggaaa gaaaaatgtc caatgaaatg gcaatctgtt ttgaagatgt cagtaccggt
    32041 tatggtaatg tgttggttct ggaagacatc aacttttgca ttcaggccgg cagttttacc
    32101 gggatcatcg gccctaacgg cggtggcaaa accaccctga taaaactgat gctgggattg
    32161 atggagcctt ggcgcgggaa ggtgaccata tttggtgaaa agccccagga catgagacac
    32221 ttgattggtt atgtgccgca ggctgcccat atcaaccaac ggtttcccat atccgtaatg
    32281 gaggttgtcc tgcagggacg attgggagaa aaaccgttct tttttcatcg atatcgtcaa
    32341 tacgattggg acaaagcccg gtggtgtctg cagaagctgg aggccgccga cctggccaac
    32401 cggcaaatcg gtgagctgtc cggagggcaa tggcagcgtg ttctgattgc acgcgcgctg
    32461 gccgctgaac cacgacttct gctcctggac gagccaggca atgggcttga ttccaatgcc
    32521 agcggtaaag tctacgaact gttggaggaa ttaaaccagg agatgaccat tgtaatggcg
    32581 actcatgata ccatggcgat cagtacctgc gtgcgcgata tcgcctgtat caatcaccgt
    32641 ctccattatc atggagaacc ggaactcagc ccggatctgg tgatgcagct gtacggatgt
    32701 ccggtcgaac tgattgcgca cggtgttcca catcgggttt tgcatcaaca tggaggacaa
    32761 caatgataaa cgccttatta cagtacggtt ttttgcagaa tgcggtgatg gcctctttac
    32821 tggccagtct ggcttgcgga attatgggac caatgattat cgagaaaaaa ctcctgatga
    32881 tgagcggcgg tattgcgcat accgcttttg gcgggattgg tttgggatac tttttacaaa
    32941 ttgaaccgat gtatacgggt ttgggttttt ctttgctctc tgctgtgggt attgcgcaaa
    33001 tacagcgccg ggccttggtc atgccggacg ttttgatcgg catgttctgg ccgttgggca
    33061 tggccctggg aattctgttt atttccttta cccccggcta tcctcccagc atggagagct
    33121 atttgttcgg cgatattctg atggtatccg gcacggattt gctggttatg accagcctcg
    33181 atggcattgt tttggctacc attttggcct tttatcattt attgcaggcc tatctgtttg
    33241 acgaagagtt cagtcagaca cgaggactgc cggtcgccct ggccgaagcg ctgacctatg
    33301 ccatgattgc catcaccgtt gtggtattga tcagagtggt ggggatcatt ttaattttag
    33361 ccctgctctc tgttccgccc gcgctggccc gcctttttcg ggatgacctt ccgggcatca
    33421 tggttttgtc ctttgccata ggtgtgtttc tctgcctgac aggcctggcc ctttcctatc
    33481 attacaacct ggcctccggc gcagcgattg tcatactcgc tgtcataatc tataccttgt
    33541 tccagttgct tgacaaaacg ttgctggcgt ttgtccggca acgacaactg taaacaaaaa
    33601 tttctgtggg acgctgatta tccccggaca ggagtgaaaa cgatatggat gcggaacaat
    33661 tatgtgcccg gctggctgcc aaaggataca aactgaccaa cagccggcgt aagatactgc
    33721 acagcatggc ggctcaaact gactgggtga cggccaaatc gttgcatgac aagctacagc
    33781 agcagaacat tcacattgat ttatccacca tctgcagaaa tcttgatttg atgtatgcca
    33841 tgaacatgct ttgccgggtg gacaaagaac gtaacggcac gtttgcctat cgtctgcagg
    33901 atatgcaaaa ccatcatcat cacctgatct gtttgtcgtg cggcacgatt gtaccgctgg
    33961 aatactgtcc cctggaaagg ttgccggaaa gacaaaccca aggcttcagc catctggaat
    34021 gccaattcga ggtctatggc cattgtcagg catgccagag ggcgattcgg aaatcgtgag
    34081 agatgaagca gtttgttttc aggccccaaa ataattgtca agctttttca tggatagcgc
    34141 cgattgggca aatatcagca cataatccgc attcaagaca taggctggaa tcaatagagg
    34201 ctttgttttc aatgatcaaa atagcgttta ccgggcaggt atcgacacat attccacaac
    34261 caatacaact gttatctaca tacattttca tatctcccta aatctttcat aagcaattaa
    34321 acaaagagtt ggcgaggaat tcttaaaaat cctcaccaac tgttgcaaaa ctcagcttta
    34381 aacattatcg atatttttgc ctgtcttggc agcagatatg gttctgcgga tgtctgccag
    34441 ttcttcttcc aaaaatctgg ctcgttcttc caacccggcg acggtgtcat tcgcgtttac
    34501 tccgtaatat ggctgattaa accaacccat gccgcgtcct ctgccgccaa agcctcgcat
    34561 tccacgtgcc cagccaaaac atcttcccgg ggtattgctt agatatcctg gattggcata
    34621 tcctgaacaa tatcccatgc cacgtccgct tgcagggcct ctgccgagcg gaccggtacg
    34681 atctcctctt ggcataacgg ttcacctccc ttccagtaac cttaatgaat tttagcaaac
    34741 aagagaatca aaggttaccc tgacctcttc tacggcgttg ccgatgttgt cgcattatac
    34801 cagtgccggc aagctgtccc gtcagaaaca agctgatgat ttcttccagg gagcctgtta
    34861 tattagcaat aatttgaata ttccgtccct gcagcgccat gacttgggtg cgtgaaatcg
    34921 caccgcaaat tacaacttct gttgcccaac gggaaagctc ttcggcccgc tggaaaggaa
    34981 gttcgatgtc aagacgcatc gtttctgagc tgatgacttt gttattgaca atgtctatca
    35041 gcaacagatt tttggaataa tcaaataccg gagctatgca attctgccag catgatatag
    35101 caattctcat aacagttaaa atataatgca aataacgtgc catattattg tttttctatt
    35161 taatccctta atatcgaggt aaatggcaca tcaaaataat ctaatccgca aaatacattg
    35221 caaaatgcaa cttaaatgat ataataaaat agcaatgtgc aatgtttgct gagcaaaggt
    35281 gtagagcagg aaaaatgaaa atagaaaatt taaaatttaa taacaacaat tacgagatca
    35341 ttttggattc gatcaacgaa ggtgtcttta ccgtagatca ggattggaga atcaccagtt
    35401 ttaatacggc tgccgagaaa attaccggga tttcaaaaaa agaagcaatt ggccatttat
    35461 gctatgagat tctgcgggcc aacgtttgtg aaacgaactg cgcactaaag cacacttttt
    35521 tgtatggaaa gccgattata aacagcacgg ccaatatcat agacaactgc gggcgcaagg
    35581 ttcccatccg gttgtcgacc gccattctgc gcaatgaaat cggggaaatt gtgggtggcg
    35641 ttgagacttt tcaggacttg agttatattg aacaattgca gaagcaactg gaatcaagct
    35701 acagttttga agacatcatc ggccgcagta cccgtatgat tgagttattc agcattttac
    35761 cgcaggttgc cgagagcagc agtaccgtgc tgcttaacgg tgcgagcggt accggcaaag
    35821 aactgtttgc ccgggcgatt cacaatctct ctccccgcca taacaagcga tttgtgggag
    35881 ttaactgtgc agctttgccg gatactcttt tggagagcga attgtttggc tatcaagcag
    35941 gagcctttac ggatgctaaa cgagataaac cgggtcgttt tttactggca gacggaggaa
    36001 cccttttctt ggatgaaatt gctgatatgt ctacggccat gcaggttaag ctgttacggg
    36061 ttttacagga acgtgtcgtc gaaccgctcg gctcactcga accgattccc gtggatgtgc
    36121 gtgtcattgc cgcaaccaat cgtgatttga atgaaatggt gaggaacggg ttgtttcgtg
    36181 aggatttata ttaccggatt tgcgttgtac atatgcaatt gccagaatta aagcagcgcc
    36241 gagaggatat cccgctgttg attgaacata tgatttataa atttaatcgt ttacaaaata
    36301 agaacattgc cggggtctcg gatgaagtta tggcggtttt gtttaattat gattttcccg
    36361 gtaatatcag agaactggaa aacatgatag aacatgcttt tgtgctgtgc cgcggggaac
    36421 tcatcgaact tcagcatttg ccggctgaaa tccgtgcttt gtgcaaaacc acagtgaaag
    36481 aacttcctac attgaatctc gattccatgg aaaaacactt gattatcgaa gcactgcaaa
    36541 aacatcaggg acatcgaaaa cttgcggcac aagacctgaa tatagatgta agtacgcttt
    36601 atcggaaaat gaaagccttg gacatacagt atcaaaaaga aaacaacgaa aataaagact
    36661 agaaagaagg caggatgatg aggtgttttg gaccggttcc gtcgcgtcgt ctgggcagga
    36721 gtctgggcat aaacaatata ccgcccaaaa tttgcagcta cgcctgcgta tattgtcagc
    36781 aggggttttc ctctcatatg caggccggac gacaggcgtt ccatgatccg gacgaacttt
    36841 tcagtgaggt aaaagaaagg gttgaacgga tggagcgcaa cggggagcag attgattatt
    36901 taacaattgt tccggatggt gaaccaacac tggatgtaaa tctgggggaa ttgatcagaa
    36961 aactgcaaag tcttaagctt aaaacagcgg ttattaccaa tgggtccctg atcgatcatc
    37021 cgcaagtgag gcgtgaactt gcggaagctg acctggtttc tttaaaagtt gatgctgtta
    37081 atgaagatgt atggcggaag gtgaatcgtc cgctcagaca attacacttg gaaaaaattc
    37141 aagctggtat gcttgcattc agtcaggtgt ataaaggacg gctgataagc gagaccatgt
    37201 taattaaaga tgttaatgat acgcaggcgg agtgtcaggc catcgcaggt tttctaaccc
    37261 aactgaagcc agctgttgcc tatatctctg tgccgacccg accgcctgcc tgcaaatggg
    37321 ttaacaagcc ggacgaagag aaattacatc tggcttatca aatatttgcc aaagatttgt
    37381 ctaaggttga atatctgaat gagtttgaag gcagcgattt ttcctttggc ggcgatgttg
    37441 cccgggacat ccttgatact tgtgcagtcc accccatgcg tcaggacgcg ttgcaaacat
    37501 tgctcgggaa tgcaggtgcg gattggcaaa cggtggagga cttgataagc tcgaagcagt
    37561 tggtggagct tgtctatgat ggaaacaagt tttatatgtg taaactatat gaaggataca
    37621 aacgataaaa aaacttttaa tggatactga cattgacccg gggatatttt ataatgagca
    37681 tatgttcttt actttaaaca atacagaggg gagtttcaaa gatgaaatca acgaatgtat
    37741 taattattgg cggatcagca gcaggactga gcacggcaaa ctctgtaaaa acctggcacc
    37801 caaataaaag cgtaacggtg gtgcgtgatg tagcttatac ggttgtacct tgtggaatcc
    37861 cttatatata cggaacgctc aaagcggtgg aaaaggacaa aataccggat gaaggatttc
    37921 tcaaaaaagg catcgaaata gtggtatcca aagtaagcag cattgacaga agccagcatt
    37981 tggttacgtt ggcggacgga gagacgataa gctatgaaaa actggttttg gcaaccggtg
    38041 ccagaccggt tgttccgaac attcccggtg cacaattgga aaacatattt ccgatttaca
    38101 aggacccgga ctatttgcag catttggatg atgtattaca gcataacaag aaggttgtcg
    38161 ttatcggcgg cggttttatc ggcgtcgaga tggctgaaca aattcgtctc aagggcgatg
    38221 catacaatgt aaccctgatt gaggccttgc cccattgtct ggcggttgcc tgtgaagagg
    38281 aatatggcca aaaaattgaa atggaacttg aacaaatggg gatcaaggta atgaccaata
    38341 ccttggtgga agcatttacg gggcaacaaa aagttgaggg agttgttctg aagggtggcg
    38401 gcagtattgc cgctgatata gtcatattgg ggataggcac tcttcccaat attgcattgg
    38461 ccagtcaggc aggcttgcaa gtcgatgaaa aagaaggaat gattgtcgat gaatacttgc
    38521 gtaccagtga tccggatatt tttgcttgtg gtgactgtgc cagcaagttc tccagcatca
    38581 acggtcagcc ggtaatcatc agactggctt ctgtagcagc gtcggaaggg ctcattgcgg
    38641 ccagcaacct gttccagtta aaccgtaaat caccgggtgc gttaggagct tttgcgacca
    38701 aagttggcaa tacggctgtc ggtgctgcag gatttacgga gcggatgtgc caggaagaca
    38761 atatcgatta ttatgttggc aaaatggttt ctccagaccg gcaccctgcc tcattgccgg
    38821 gatgtaccat gcagacagaa gtaaaattat tatttgaccg acgggatgac cgaatcattg
    38881 gcggacatgt tctgggagga gtacaggcag cggatatggt gaatatactg gcgctcgcca
    38941 ttcaacagaa gcttactgcg gcagagttgg ccattatgca gtatgcgaca catcccttac
    39001 tcactggatc acccctggta tatcaggtca tgtgggcggc agaaaatgcc atcgctaaaa
    39061 aataatcatc ctcgtctaac cgaaacgata ttccattaaa agactttttt gtagtaatat
    39121 aatgggaaaa gaatgaaagc gaggagacga taattttgaa attaaccgat gctgaagtga
    39181 ttgaactgtt gcagtattcg tttgtgcgca ttgacggagc ctggttcatg ggtctggcca
    39241 aaacgcatgg gattgagacc gcttgggatg ccgacgtgga agcctggaag caatactcct
    39301 atgtgtttgg caagaaactt cgcaatgatt acattcaaga accggtatgg ccggaaagtt
    39361 tcctggaagc catcgagatt ctcagcaaag tgttaaaaat cgaaggccgg gaagtttcct
    39421 tggagggaga gcgtatcgtt gtcagagtga cagactgcga aacccagaag gcgattgcca
    39481 aggccaacat tgctgactgt ggcattgtaa ccatccatac ttatcaggga ttggtaaggg
    39541 gactgtttga aaaagacgta gagattgaag tggagcatac ccaaaacctg aatcatggag
    39601 atccttgctg tgaggtggta atacagcgca aatcatagtt ttccggtcaa gtcaagatgg
    39661 catcagcaaa gaagggggat tttataatga acgtcagcgt tacaattttg gtggaaaaca
    39721 ccactcccat cccgggattc cgcggcgaat atggttttgc ggcattgatc acggtggatg
    39781 ataaaaagat tttgtttgat acaggttccg aagatgcact gataaaaaat gccgccgctg
    39841 gaggcattga tttggcgcag ataaacgact tgatcatcag tcacggccat tttgatcata
    39901 ccggagcggt gattccattt ttacagacgg cacctggaaa aaaagtgtat gcgcatgcgg
    39961 gaatatttat ccctcgttac accgtttggg gaaatttccg gaaagccatc ggggttccgt
    40021 ttgattccca ggaaatcaca cgtaatggtg cgcagtttgt gattaccaat gattttaccg
    40081 agattcaccc cggcgttttt gttaccggcg agatcccccg caagacgcac tacgaagatg
    40141 tcggcggcag tttttatgta gaggtgggcg atcgtttggt cgaggatcat ctctacgatg
    40201 acatgtcggt agttatcaat catccagagg gcttgattat cattagtggc tgcgcccatg
    40261 ccggcatcat caataccatt gaatacgcaa ggcagcaaac cggacaaaag aaagtccggg
    40321 ctttcattgg cgggacccat ctggcgggcg cttctgaaga gagaatgcag gaaaccatca
    40381 aggctttgcg ggaatttgat gtggaacaga tcattgcctg tcactgtacc ggctttgaag
    40441 caaccatgaa actccgtaac gaactggggg acagagtaat gaaaggcgaa accgccatga
    40501 actttcagta ccaatagttg aacaaacaaa taaaataaga aacaccttta gagaaatcat
    40561 tctctgaagg tgttttttta aacgaacgta ctgatgcatt aggtttattt ccagagatcc
    40621 agaacctttt tgcgataata actggtatac aaaacagccg ccggattgtg tcccagtacc
    40681 cgatccttga caatgattgt tgttaccgga gcctcagaat attgggagaa aagtatgtca
    40741 tgtccaacgc acaatccaca gatgaggttt aaatcagttt gtttttggtt cagcaagagc
    40801 gcttgcccaa ttggattgca cattacttca tccttgctgg gatcgatttg ttccaattcc
    40861 agttgttttt tgagcaatcc gccatttttg caaatgaccg ttgtcacatc aaagtctttt
    40921 tttagataag cagcgatcat ttgtgcttct gcaatcaaac cgatacaggt tgcgataccg
    40981 atttttgaat acgacatttc gcgggcaaaa aggcgaatct cttccagacg ggtagcttgc
    41041 tggtaagttg ttgcttcaat tgcggcagaa gcttttacca gtttataatt gtctggtttt
    41101 ttatatattt gtatgattcc ctggtttatc tccggataac aatcccggcc ttttttacaa
    41161 cgttgttttt gacagatgga acaatcattg ccgatcggcg gttgactttt ttctggttga
    41221 aaaacatcct tgtctttatc gatgatgatc acccctttcg ggaaataaaa tacttaacaa
    41281 tggagaggga gggacagctg atccctccct caaagaacat tttctacttg ttgcttgtat
    41341 tgttggggtt atttgccccc tgaccccggc cgccgttttg cggaccgttg ttgccactgt
    41401 ttctgcgtcc cagacctttt ccgggagaat tgtttttatt attgccgcga ccggcagcac
    41461 agcctccctt taaacgaccg gtaccagcgc cctgacccat tgggccggtt ccatctttgt
    41521 tcggcatctg tttcactcct tcctgtcacc tctacccgtt tacaactgtt gtaagatatt
    41581 ttcccatacg ggaaaaatga aatttctgat ttgtggctgc gtggcgggga ttcttgccgt
    41641 tgcaatacaa tcagcaatcg tggtgctgaa gggtatttta ccggccacgc aaacgccttg
    41701 tgcagttgcc cagtcttcta ttgtttgact tgtttccggg ttaagatccc atttattgat
    41761 gattaaaacg gattttatac cccgtaattg aattaacgca taaacccgtt taaggtcatg
    41821 aaatcctgat aaactgggtt ctgtaaccaa tacgatcaga tcggtttcca ccatggaaga
    41881 aattactgca caaccgattc ccggcgggcc atccgcgata atcaaagaaa cattgtcagt
    41941 caaagctttt tgacgggctt gagtttttac ctgggccacc agtttgccgg agttttcctc
    42001 tccaggccga agatcggcaa aaaacaagaa ttgacctggt ttgaattcag aacgaaacca
    42061 gtgcccggaa ggtcggggat tcattttaat cgcttccacc gggcaaatcc tggcgcagaa
    42121 accacatcct tcgcaatgca gaggaatgat ctggtaatcc tcgttgatgg cctcaaaacg
    42181 acaaacttct ttgcaaagac cacagctggt gcagatttct tcatcgatgc aggcactgta
    42241 gccggagctg aaatcatgcc cgacaatgtt attcggtttt aacaaaatcg gcagattgga
    42301 ggcgtcaaca tcataatcac aagcgatgca gttgtcggct agagcaataa aggaagaagt
    42361 aagagacgtc ttgccggttc cgcctttgcc gcttatgaac aggatttctt tcatgctgac
    42421 acctccttga tacggtgcaa aatatcgctg atgataccac gcagcgcagg catggcggcg
    42481 aaaggatcca aaccttgcat ataggcgcgg gccaattctg cactaaatgg aatccttgcc
    42541 aggataggaa aaccccagcg ttcggataaa tcgagcagtt ccccgtcatt accctgccag
    42601 cgattaatga ccagtccacc ttgcataccc agcattttga ccgcttccag ggatagttcc
    42661 aaatcatgtg ttccaaaggg agtgggctcg gttaccagca aacaataatc actgtcacgt
    42721 atggcttcaa tcataggaca ggttgtcccg ggtggacagt ccaccagtct gattgcggcc
    42781 gaaagctgtt cattctttac cgctttaatc agtgggggac tctgggcttc gcctatattt
    42841 aacctgccgg tcaggagagt gccacttgtc tgtagcgggg tttcttcaat taccccgatg
    42901 gggtgttcga tctcatcgat tgccgcatag ggacataaat aactgcaagc gccacaggag
    42961 tgacataatt ctggaaacac catcactttg tccgcaagca cgaccagagc attgaattca
    43021 catatctcgg cacataaacc acaataggtg caatactccg gcaaaatctg cggaaccggg
    43081 cgataaactg tggtggttgc ggatatgggc tgcagaaagg ccaggttggc gttgggctct
    43141 tccacatccg catccatcag gacagcgttt ttgtccgcca aaaccttgat gatgcaggtg
    43201 gaaagcagtg tcttgcccgt gccgcctttg ccgctggcga ttgcaatttg cataaaggac
    43261 tccttcctag tggtgacagt cgcccgcgtg agagtgatcg gaacagactg cccccgtact
    43321 tttcaattca cctttttcat acaaggcgat aacatcctga atgcttcccg atactccaac
    43381 aattaattcg atcccgctgg cagcaaagag ttcctgagct cttccgccca ttccgccggc
    43441 gatcaccaaa tgtacccctt gctttttcaa aaaaacgggc aggaagcctg gttcatgtcc
    43501 agggttgggc agggatttta acgctttggt atctgtgtca tacatggcaa atacttcaca
    43561 atgtccaaaa tgaggacaaa ccatattggc ttccgctgga atagcgatta acatgtatac
    43621 tacctcctgt ttcgataatt ttttactttt ccattcaaga ctgtgacata attcgttttg
    43681 cttaaaagac gttctctatt atactgttgc tttgtttgtc tggcaaacaa agcaacaata
    43741 ccgggtttaa ttgctggggg tcaataattc ctgcaattca ccgttctcat agcttcgcag
    43801 agcttcggca accgtttttc cagtgattcc ggaaaagatt tttacaccgg cctgttcaat
    43861 gacagcgaag gccttggggc cgattctttg cgagagaatc agattaacct tgttttcaag
    43921 cagggtctgt accgctcctg ttcctgcacc gtgtgcggaa tcggtaacgg tgttgggcag
    43981 agctgtaaac tccttgcttg ccggatccca gatcatgaaa taggcacaac ggccaaacct
    44041 ttcatctaca agagaagatg gggttgaacc cgttgaacaa acagcaattt taccagacat
    44101 catttcacac cctttcgatc aaaatttaaa tgagcatatg ttcataacca tatttaaata
    44161 ataggatggc actgtcttct tgtcaagtat gaatgttttt aatggtcagg gtttacggtg
    44221 atgcttttat aaattttgca attgcttcag gttatatgtg cacaatataa atatgcttgt
    44281 atatttgtat tgtttaaatc gctatgatcg aataaaatga taatggttat catttcgggg
    44341 ttgtttgggt tcatcgcata gagaattacc cgcttcggag agattagctt gattgcctgc
    44401 ttttttgaag atataagcag gaatcatccg cttttgggaa aactttagtg aatgagtaat
    44461 gtttttgaaa tccttgcgga aatgaaggcg attggtttga aattggtttt ggtggggaac
    44521 cccaatgtgg ggaagagttc cgtcattaat ttgctcaccg gcagtcagtt ttttgcttcc
    44581 aattatccgg ggacctctac ggaaatcgaa aaagcggtat taaaaataaa ccaagtggag
    44641 tttgcgtttt acgatacatt aggggtatat tcgctctata cagatcggga agaagctacg
    44701 ttgacacgta ctcttttgga agaacagaac tacgatttga ttatcaacat cgttgatgcg
    44761 agtaatttgg aaagaaatct ggtcttaacc tatgaattgc tggagcgggg ctatccctta
    44821 ttggtgctgc ttaaccaggt ggatcgtacc cgggagttgg gaatacagat tgatgtggcg
    44881 gaacttgcaa ggattttaca gcgtccagtt attgcttttt cggctaccaa caacgaaggc
    44941 gtcagcgagt tcagacagtt tcttgaggaa ctgggtaaaa aaccggatgc tggccgggaa
    45001 aaagaattta cccggcaagg tttgccgcgg atcgttttaa tggaagacac gagttgttca
    45061 ggaaattgtg gaagctgttc aacatccaga gaatcctgca gcattgacct tgatttgcaa
    45121 ctggcggaaa aggcacggaa aacatctcag gcagtgagca cattcaaaac gccggctaag
    45181 cacagcctca tcaatgatgt gcaatttttt attgaccgac cttatgttgg cacgattagc
    45241 ctgttgctgc ttgcctatct ggcgctggtg gttttgacga aatttatcag cttcagcgaa
    45301 ggaccaatta gtcaattact ggagccgttg agcaacatgc tgcacaactg gattctggcg
    45361 attatgccgg cagggttttt aaccacagta ttggccaagg cgattccgga aggactggtg
    45421 atcccattta cgattattat gccggcgatg ctgatggtga gcttgctgat ggcagtattg
    45481 gaggacaccg gtttgttgcc tcgttattct gtggcaatgg aacgatttgg ccagttgttt
    45541 ggcgtatccg gacaggcagt cattcctttg tcacttggtt ttggctgtcg cacgccagca
    45601 gtaatggcga cccgtatcct gcccaatgat gcccagcgct ttattgtcat cacgctgtta
    45661 agtattgtga ttccctgcgc ggccacggta ggagttatcg ttatgatcag tgccaagttt
    45721 gaggcttcat tgacggtgat tgttctgacc atgctggtag tgatgactct cctgggtttg
    45781 ttgctgagtc gtctgagtcc ccgggaggaa gcttttatct atgaactgcc gccgctgcgt
    45841 gtcccgagga ggaaaaacgt ctggagcaaa atcaagcttc gttttgcagg tttttttact
    45901 gaagtactgc cactgctgct ggtgatgagc atcgccattc gagcattaat cgaatctcat
    45961 ctgctggaaa tattcaacag catggatgcg ctgaccaaag tgatctttgg gattccggcc
    46021 gaagcttttg tagccgtatt gattaccatc tttcaaagat atctggcgcc cctggtattg
    46081 ctcaatctgg atctttccgc ccgggaagca accattgcgc tttccatgat tgcgctttcc
    46141 ctgccttgtt tgccggtcat ggtggtgacg gtgcgggaga tgggcttcag cagtttaacc
    46201 aaaattctgc tgatgggctt tatcacttca gcacttgtgg ggatttcact caatctgatt
    46261 ttgccgttct aggaggggaa aaaaatgctg gcaacagaga tgaaaaaagg gcaaaaagcc
    46321 atggttcgtt ccttcgggag aggcagaggg atggaaagaa gactcttcga gatgggcatt
    46381 atgccgggaa cagaaataga attactggag aagcacccgt tcaagggacc gctattgttt
    46441 caggtaggac aagcccgcat cgtactgggc cggaacatcg ctgcccattt ggaggttgaa
    46501 attacggaaa tatcgctccc cagccacttc ggatcataag ttggggcggc tgtcagccat
    46561 tgggggaatc agctttgttc agctgttgat gctgcagcat ctgcacagct tcggtgatgc
    46621 ttttcccatt tgcgcctgta aaaacatcta tcccggctct ttgcagtata agaaaagcct
    46681 tggggccgat ccgactgaca ataactgcct tcacgccagc ttgaaccaag gctttggcgc
    46741 cgatggttcc ctgtccctgt tctgtgtttg ctgccttgac atccagtaac gaaaaagcct
    46801 gactggcggg atcccaaatc caaaaacaat ggcatttgcc aaaacgttcc cgcactggat
    46861 cggaaagatc cgtactttcg gcacaaactg cgatctttgt ctgatggaca ggaggattgt
    46921 ccgttgcact caattccagc ccactgtcgt aaaaacggca gccttccatg taatccagct
    46981 gacgttcacg ccgtcggcat tgtccctgac ccaaacagta actcccacct tcgatgcgga
    47041 tggccttgcc gttggtaaat gcatccgcaa ccttggcata agcttcggtt agaatacgct
    47101 ggaaagtagg acgggaaacc tccatcatgc gcgcgcagtc ttcctgttcg agattgaggt
    47161 ggtttttcag tcgaatggct tcaatttcct caactttaaa gataacctcc tccatatccg
    47221 ccaatggtac gccgagggga tgatatgctt ttaccggggg caaaaaagcg actcttctgc
    47281 attttgattc tcttgccaac gtagtgtacc tcgcttgtct cgtttcattt tgaccagtat
    47341 tgttaagttg attctatctg tatttacagc gaaacgtcaa gtgaggaggt tttttccgca
    47401 ttataacttc cggcaattga catactggga ggatgcgagt attctaaata tgaatatgag
    47461 catatgctcg ataataatga tgtcggggtt gttctatcat ccggttacgg aaagggatga
    47521 gatgaacaaa aatttcatac tcaaacgatc agatgacgaa aggagatcaa gattaatgag
    47581 tcaaaacaaa aaaaatgccg gcaatgggca ggggatgcgt attgaagaag gtcccatgct
    47641 ggttggcagt ctgaacaaga tcaataaggt gatcgccgtc atgagcggga aaggtggcgt
    47701 cggcaaatca tccgtttcct ccatcctggc cgcgtctttg gcacaaaatg gatatcaggt
    47761 aggcattctt gatgccgaca taaccggacc gagtattgcc aaggcctttg gcgtgaaggc
    47821 aggaagttgt accgtttccg aatatggaat gaaccctccg ctcagcaaag gtggcattaa
    47881 aacgctgtcg atcaatttgt tgctcgaaaa cgaggatgac ccggtaattt ggagaggtcc
    47941 tttgatcgcc ggggcggtca aacaattctg ggaagagacc aactgggggc atctggatta
    48001 tttggtggtt gacctgcctc ccggaaccgg cgatgcagcg ttgacagttt tgcagagcat
    48061 tcctgtcagc ggggtggtca ttgtttcctc accgcaggat ctgtccctga tggtggtcag
    48121 gaaggccatt aaaatggtac agaaaatgga aattaaaatt ttaggcttga ttcagaacat
    48181 gagctatgta gagtgcccgg attgtggtaa aaagatcgag ctctttggta attcctccgg
    48241 agataaggtg agtgatgaca tgggcattgc ttatttgggc gaaataccct gggataatca
    48301 gattaacaag ctgatggatg ctggggaaat cgagttttat gaaaaaataa accgcttgga
    48361 agccatcgtg aaaatcattg aacttgctta aaaattggag tgtggacatc ttttacagaa
    48421 acgatgcaga gggattgcct ggttgaaaca aattgcataa agttggctat aaaaaaacgg
    48481 cggatgaatc gaaatcatcc gccgtcttgt tttcatttag ggtctattta ttttactctc
    48541 tcccagattg cggctgcgcc atggccgtgg ccgatacaca tggactcaac gatgtagtct
    48601 acatcagcaa agtcaggatc cttgaacata tagaatatgt cgcaggcgat tctagcaccg
    48661 gtgcaggcca ggggatggcc gatggagatt ccggaacccc atacattggt gcggtctttg
    48721 ggcagattca gaaccatatt gcagcaatag tgagcctgtg aagcaaaggc ttcatttact
    48781 tcccagaggc cgatttgatc catggtcatt ccagctctct ttaatacttt gggaatggcc
    48841 agagagggac cgatacccat aacatcagga tcacaaccta ttacagcata gtttacgagc
    48901 ttcataacag gcttcttatc caaagctttg gcgccatcgg cagttgccaa taccacaccg
    48961 gcagcagcat cattgatctg tgaagcatta ccagcggtaa ccgttgcttt gtcatcctgg
    49021 gcgaaaaccg gtgccatggc agccagagtt tccatggtgg tttcagcacg taaaccctgg
    49081 tcgcgggcta cacagaattc caccagttct tcaccttttt cagccgcagc tttggccaga
    49141 taatcactag ctacataagc accatcacgt ttgacttcac ccttggcacc tttggccgga
    49201 actttggcaa taatggggat gatcgcatct ttgtttttgc ccagtccgcc attggctgcc
    49261 tgagcggcgg cgcatttggc atgggaagca caagccaatt catcctgcat ttcacgggtt
    49321 acgccataac gacgagctac aacttcagct gtatgtccca tgttgatgga tctcaaatcc
    49381 atgtatttgc ccaagtcagg atggacatcc gcgccagcgc ccatcggaat gtggctcatg
    49441 tgctgggcac ctccggcaat ttgaacatct ccgccccagc cagccatcat cgtagcagtg
    49501 gcaaaggaaa tggaattgat acctgatgca cagaagcggt caaccgtaca tccgggaaca
    49561 tcgtaggaac cgtcggaaag gagccaggca atgcggccct ggttgaggcc ttgttccatg
    49621 aaagcattgg cggtggcagc ccagactatg tcgtcaatta attcagcctg tttggctttg
    49681 tcttcgcagc cgattctttt tctcagctct ttaaatacaa tagctgataa ttcatcagcg
    49741 cggacattgg tgaaccagga ttgttttcct gcacgggcaa cggccgtacg tgcagcttct
    49801 acgacaaata catctttcat ctaaatggta tcctccttta aattttggta agattgtaac
    49861 ccaatcagtt aactattact gtttcgcgat aaatgttcaa aatccttctt ttattaaaaa
    49921 aggtaattaa tatttttaat aataattaat aattgcatca attcaatgtt tatctgttta
    49981 tcttttacct tatgttgtaa tttcattcat gcagattaat gatttttttc acgctggaat
    50041 tgtgttatac tacgttaata aaaacaagct ggaataatct gaatggattt gtgaaggggg
    50101 aagaatcatg gaagaactga ccaaacaagt gcaatttgaa atgctccgca ccgtattatg
    50161 gctggcagtt gcgctgggca ttgggattgg tgcttatttc attatttggt agcctcccgg
    50221 cacaaaacac ccaacagcaa aagagcctct gctttatgca ggggtttttg atttttgggg
    50281 acagtcccca aaaatcactt ttaaatagtt gacaaaccgg taacaatctt cttataatca
    50341 ttaatagata cccaaggggg gtatgagatg aaagaagaaa ttaacgatca agaagcgcgt
    50401 aaaaatgtcc tggtaagact cagaagaatc gaagggcaag tgcgcggcgt ccagcgcatg
    50461 gtggaaaatg aagcagagtg cagcgaaatt atgaaccaga tcgctgcaat caagtctgct
    50521 gtcaatcacg tggggctgtt ggtctttgaa agtcatgccc gtgaatgcat agcccgctca
    50581 ttggatgaaa aagataatga gcagagcttt gaggaaatcg ttaaaatcat gggccgattt
    50641 atcaaatgat caacctttgc agtaaaacaa gaacattgaa ggaggaatca taatgtcaag
    50701 tgtgaaggca gttaacacag aaagcttcaa taatgaagtg ttaaaagcaa acaaaccggt
    50761 tatggtcgat ttttgggcgc cttggtgcgg accctgcaaa atgctcgggc cgatcgtgga
    50821 taccctggcg gaagaaaacc tggccaaact cgacgtagcc aaagtcaatg tagatgagaa
    50881 caaatcactc gccgcccagt ttggcgtacg gggaatccct accctgattt tctttaagga
    50941 cggtcaggag gtaaaacgcc tggtgggtgc ccaaggcaag ccgcagctgc aaaaaatgat
    51001 tgatgaagtc attggctaaa gcaactgaac gtgcagaata aacaggcagt ctgtctatgc
    51061 tggcagactg ctttttctgc ccatcgggag cgcagcttgt tgagatcgaa tcgatttgtt
    51121 aacataacca taccgtatta tttattgatg cgaagcaatg aattcgatga acattttttg
    51181 taacatgaaa tcgtaggctg gaacaggagc agtgagggtg gcagaaatta ctgttgtgac
    51241 atttaatatc agacacggtc aagggatgaa cgggaaggtt gatctggcca aaacggaaca
    51301 ggtattggcc cggtgtcagg cggatatcat ttgcctgcag gaagtggatc gcggcagaat
    51361 gcgcagcggg tttcaggatc aggccgcccg gctggcggac accttgcaat atcagcatgt
    51421 cttcggcccg gttcccaacg atcgcagcca atcatacggc aatgccatct tgttcagaca
    51481 taaaatgtta caacaaaaca actatcctct gccggcagaa gtgccggagc gggccatgtt
    51541 atcaatagag ctggaaatag gggcgcaaaa cctggtgctg ttcaacacac atttgggcct
    51601 gaaccgtcgt ctccgcagcg aacagatcga acaatttatt attccgctca tggaccgcat
    51661 cgggttgccc gccattttat gcggggactt taacgaaagc ccggaagacg agggggcggc
    51721 ccgtctgctc cagtgctggc aggatagttt tgtggccaac cggacaccga tttgctgcag
    51781 ctatccggca gaccatcccc ggcaaagatt ggactacatc ctgtgcaatc aattctgtca
    51841 ggtacgggag tatgcaatcg tggagagcct ggcctccgat caccggccgg tattggcccg
    51901 gattgccttt tagcacgcct tatttgccgg gcagtttttt gagaaaatcc tgaatatgct
    51961 tttcataacc gttgctgccg ggctgataaa atttgggctg gcc
//
LOCUS       DAOTHZ010000006        44536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_868_length_44536_cov_7.674131, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000006 DAOTHZ010000000
VERSION     DAOTHZ010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 44536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44536
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_44536_cov_7.674131"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(521..1399)
                     /locus_tag="P5549_01670"
     CDS             complement(521..1399)
                     /locus_tag="P5549_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW11812.1"
                     /translation="MRYEGNIFRPFSEANSYLLQCTIGCSHNRCTFCGMYKDKKYRIR
                     SLEEIKTDIKMAKDYYGDAEKVFLCDGDAIAIETDILLEILAELYKTFPRLRHVGSYV
                     GPQSTLGKSLSELAALRAAGLTKAYLGVETGDDALLKEVKKGVTYDEMLQAGRNLVNS
                     GINLSSMVLLGLAGKGPRSAEHALATARITNAMNPQYLAALTVTPVPGTPLFRQVQEG
                     TFQVLDPFETLEEMKIIFENITIDNLHFVGSHASNYLPIKGTLQKDKQQMVETVNKVL
                     ESRDNRYLRSEKMRGL"
     gene            complement(1430..3490)
                     /locus_tag="P5549_01675"
     CDS             complement(1430..3490)
                     /locus_tag="P5549_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRW11813.1"
                     /translation="MKFISKLLLAAGAILLFLMFFYTGVSLTQTTPKLAKFVTGISNW
                     EYEVQGQTAVFYHDGKKMGNLGYQREYSEEFPKFMKDAVVAVEDKRFYEHSGFDSRSI
                     GRAIYVNIRSGNKAEGGSTITQQLARTLFLSQEKTYWRKIKEVFIASAIEDKYTKDAI
                     LNMYLNEIYMGRGCTGMGIAARAYFGKDVFDLSKAEITALVGIIQSPEYYSPGKNLEG
                     LKKRQQTVVNLLEEQGLLTAQEGQAIFQEELAFEPFRQSLSAHPYYMTYLARQLEKEL
                     GAQKLYQGGLKIYTTIDNRMQKAAEYAVVKQAATFAARGITAQDVALVAIEPQTGAVR
                     AMVGGVEWDKNQINMAVEPRQPGSAIKPLYYAAAINEGVIQADTKLNNKPRSFNGYTP
                     NNYAVSPEETTVRLAIVNSYNVASVEVLDMLGVNKAIKYLEKYGITSITKEDHNLALG
                     LGGMSNGISPMEMASAYTMFANGGTISPCYSIERLVGNNGETLYAGRGSAKKVVDSST
                     AATMDSILKSVVAYGTGTPAQISIRSGGKTGTTTQSRDLWFMGYTSQLSTAVWVGNSN
                     NQPVGGSRVSGGGTSALVWRDFMNRLISQGVLSVPSNQEPLEQAPVQEETPTEEEPLE
                     ELPPDAENLQPESPQPESPGDATNPDRPDSNDNEQPEDLPLPDVGNQTDNGANDAIEM
                     LEIQ"
     gene            3798..4607
                     /gene="minD"
                     /locus_tag="P5549_01680"
     CDS             3798..4607
                     /gene="minD"
                     /locus_tag="P5549_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRW11814.1"
                     /translation="MGNILEECQVYVVTSGKGGVGKTTTVANLSTGLAKLGYKVVVMD
                     LDIGLKKLDLIMGLENRVIYDIVQVAEGECTLKQALVKDKRFPDLFMLPAAQTRNKED
                     ITIEQVKAICDELREEFDFIFIDSPAGIEQGFKNSIAAADKAIVVTNPEVSAVRDADR
                     IIGMLESAGFKDIQLLINRLRPDMVKKGDMLSIEDLIEHLCIEPLGVIPEDKSVVIST
                     NRGEPIVLGNTPAANAFSNIVQRLTGEDVETMVIPEDTFWTKVKRNLFGAS"
     gene            4622..4873
                     /gene="minE"
                     /locus_tag="P5549_01685"
     CDS             4622..4873
                     /gene="minE"
                     /locus_tag="P5549_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRW11815.1"
                     /translation="MMELLRKLFLGEQNTSRKQANDRLRVVLTHDRLTVNADLMETIK
                     EEILQVIAKHIDIEGSPEVNLITEGRHAALDISIPLKGR"
     gene            4881..5549
                     /locus_tag="P5549_01690"
     CDS             4881..5549
                     /locus_tag="P5549_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRW11816.1"
                     /translation="MAKLDVKGKNNNLIFVFGAGSFEELAGLLKERVENNQALFSGSQ
                     VLFQGDGLKTLRLEELIALQRMCLDYGMVLNNMPAPQPAAAPVSAPAAAPAPAAAQPD
                     RKTHATGRDLMIFKTLRSGQKVHSEGSIIVWGDVHESAEITAASDVVVLGRLEGLAHA
                     GCFGDVSSTIFALTLTPRQLRIADRISRSSGKIVRNPYPEIAYVEGDGICIREYSNRD
                     NLNK"
     gene            5595..7508
                     /locus_tag="P5549_01695"
     CDS             5595..7508
                     /locus_tag="P5549_01695"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRW11817.1"
                     /translation="MSKRNNAPAATAQEFISSQPVTGWKQWRLPFFILLLLQAVVLLG
                     VLERPEMSLYDAWFRLQGAADPGQQVVVAAMDESSIDRLGPPAWPRSVHAQLLEKLVS
                     AKIVAFDLTFGAAKDAEQDLAFAEAIAQHGNVVLISKFYFEKDEDGDVVQIPELPLEE
                     LAASAAGMGFANMPTDPDQVVRHTTLVDTNSFDIPYPSFATAIILAAQDLNYEDLELG
                     DGYLQAGQYKVPLDSRHQAMTTFWGPRGTFQTISYADILEEKISPAYFQDKIVLVGST
                     TQDEHDYFATPYTTSNMVKSGALETPGVEVHAAVVQSYLNQSWYQRVGRGLNFLFLLL
                     LTLLTVKLVAGRGPLAGLLGVLGVSALTLLTVFVLWKNHWWLDLASPLALIFLLYVVV
                     TASDFVRAEMQRRKTKALFSRYVSADVVEQLMADPAQMALGGKKQLVTVMFADIRGFT
                     AFSENKDPVEVIHRLNEYLTVMTESILKHGGTLDKYLGDGLMAFFGAPIYYPDHVERS
                     ILVAREIQQKVAELNRQWETKGEVPLLVAVGINTGPAVVGNVGSPERMDYTLIGEDVN
                     LASRVEALTKLFSTLVLVSERSYHLLADGPVKDSLAYVGEELVKGFTHPIKVYTFTDL
                     DLHFEKSTDSGFK"
     gene            complement(7666..8097)
                     /gene="mscL"
                     /locus_tag="P5549_01700"
     CDS             complement(7666..8097)
                     /gene="mscL"
                     /locus_tag="P5549_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRW11818.1"
                     /translation="MWKEFREFAMKGNVIDLAIGIIIGAAFGKIVTSFVNDIIMPPIG
                     LLLGKVDFANLYVNLSGQEFASLAEAVEAGAPVIKYGVFINSIIDFLIVAFVIFLVVK
                     QINKMKKQEAPAEPDKKDCPFCKSSVAIAATRCPYCTSDLK"
     gene            complement(8204..11971)
                     /locus_tag="P5549_01705"
     CDS             complement(8204..11971)
                     /locus_tag="P5549_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW11819.1"
                     /translation="MSRRAEKRTGVFLLLVFLMVFVIVLPAWGNEIKTGSGLQVQSFE
                     DLTDHPEAAYIEYLASQSIIKGFDDGTYRPDEGLTRAQAACLLIRAAGITPDVTLSVS
                     FTDVPADHWARASIAAAVQAGYIKGFPDKRYHPEEQLTRAQAATLVLRLSRQSLDNAD
                     EAQFSDMNSGHWAAASATTAVEAGMMLAGSDGSQFQPEKTMTRAAMARALAVLMTEDP
                     QLYKTDLVGVLAVTKGTVTLQKDQQEPVKVTQPTTVGIGDTIVTAGNTEAEISFEDGS
                     GLLLKANTRLSIIESHGRAYIKQDGSAAVAVEWLKIDMTTGEVFGTVPATSEEEQENI
                     EESIEPENTGYLQQFNRSLLAGLNPPALLAAAKSKKATPWYKTSKTKRVKVQVDMPWG
                     VAAVRGTQFYIAQSAAGRSHITVLDGSVTADSHNLQQGQNSYQIGAGQFVEVGSSGQF
                     TAPPRPMNQNQLRQWVEVKEWLLERAQQTQINQQLNEKDMVDNILNPPSVLQQSDLPD
                     AINRALQEAQRLIRQNSGGGSSGGGGTILPVITRLQLPSDISLTVEAGQSYPLPTEVT
                     AYYSDGSSRNVSVTWSPTAVDTSRVGVQTLSGTVSGFSGAVQLPVSVTAAALGEILGS
                     YALTDRLVLDDYIYDDGVLTQDFSMNPAASLYARAGSYEIWMATTPVVEKHSLDASAR
                     VMKKLFADPERLQVVFFDENEYIVAHAGLDGSGTLVNLADRTISGTIALPEGMVAPAE
                     GMDIMLYLYGQFCAYTAPVTIAQGQSQAEYSIAVLDEAHLMPGFMPEGFQALSEPEAS
                     AGAYRHADRIPHHHGMQPMTYSRAAYNLYFDLIGSYDENLVMSGQYSEMIDVAEGSQE
                     NIDLHIETGQIVSGTIRLPEGGAAESDLDIIIQAEMISADNQEYYYAFVPLASGQNST
                     AFAITVPAEASYCLYYHLGNPVNPAEPVNSHLLREGYYTTEGSSLNAEDAVVVTVAAE
                     PAAGMDWTMSTGNLLSGSIYLTDEEAAPQDTTVYLDLSNDGFYSYAEYFLPAGSQSLE
                     YAVVLPPSSFILWLSTDYSYMDSMTLDVSNGDLEFDLALDYWSSEGEYSPYDVTAPAV
                     LVADGAYGLNSLYFGNEEFIWELYYHGSNRIYMKTGEGLWFDILNPQVNTVQDMIADN
                     AYGSSLPPIDYDDSWADILSVFSRENSFGSLDIIFFSLPEGAGVNVYDAAEGGNLLGT
                     AVCEYIDGDFVAKVELNVPEISESDLFVSFTVNQAGGQRRIPLVTLLSPLV"
     assembly_gap    12240..12339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12455..13255
                     /locus_tag="P5549_01710"
     CDS             12455..13255
                     /locus_tag="P5549_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW11820.1"
                     /translation="MAENYKGIQYETILAWVEDGIGYLQFNRPKAFNAVNYKLLIEAT
                     EALNFYSEDPDVKVIIVCGNENAFAAGADLKFVSGFNAFEARDFLDKVHDCMFALEDN
                     HKPTIAAVRGLALGGGLETVMACDIHIMADNCTLGLPEISLGIFPGAGGTQRMPRITS
                     LNIAKQYIMTGDFFDAATAYRIGLANIVVPADEVMGEALKFAKKLTKKSPLALREAKN
                     AINNSMNYDIKAGCRAEQIAWSMLFSSEDQKEGMAAFLEGRKAQFKGK"
     gene            complement(13329..14609)
                     /locus_tag="P5549_01715"
     CDS             complement(13329..14609)
                     /locus_tag="P5549_01715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW11821.1"
                     /translation="MTGEIILEKMLDVVECAVACYQMLLREEQRDYQFVAVNHTFESL
                     SGHDRSQLLGRCLGRDHFSGLFPGFDLAAWLSQSDEVSPPTPLLLLGNSCYQSFLHKL
                     DKDHLVSVFYDVTAMHSYQQKFELVFNSANALQSITTYKEDEDGIYVDVNERLCSTIG
                     LSREEIIGRSWLDINLFANSDDKHLIKLLIARDKRIDNLELITYNRQGEKIHGLLTSE
                     IIAINNQQFAFSMVHDITEFRRIEKELAEKNQQLEELNQLLSRQATRDDLTGLYNRRH
                     IFAILNKEIIRSKRYGYPLSLMMLDLDKFKAVNDNYGHQFGDFMLQKTARILLSNIRE
                     TDRLGRYGGEEFLLILTHTDLQAAEVVAQRIINAVAQASFGEDQIKITISIGVCDYQG
                     ESRDKFVQCADDLLYQAKKAGRNRFVAKSLPSQK"
     gene            complement(14773..18453)
                     /locus_tag="P5549_01720"
     CDS             complement(14773..18453)
                     /locus_tag="P5549_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW11822.1"
                     /translation="MRIEKLRFQNLNSLTGVWEIDFTEPAYRNNGIFAITGPTGAGKT
                     TILDAICLALYGRTPRLERINKSSNEIMSRHTGSCFAEIVFATGSGEYCCHWSQQRAR
                     KKAEGELQPPKHEIADLRSGQILENRLRDVAAAVEQITGMSFIQFTRSMMLAQGGFAA
                     FLQAPADERAPILEQITGTAIYSEISIRVHQRYSDKKKELELLQAATSNIKILTEAEE
                     QEMEILLKTLNEQDTAGQAQLQTLTEALAWLTRLEEREQEVKAVKLQWKAFLARQEAF
                     RPDEERLRLARQAVGLDGAYHRLMLVRRRQAEELAELNQLAAVIPAQRAAQKSADQAW
                     RDAGQRLNEAKAARKIESETARQVREMDLMIRQLAGQMEQLKTMVATTQATCQEWQGK
                     ITAAEVQQQQMAANIAAATEYLQKNQRDETLLTKFSGIRRIFEQLRDKGEVLQQKQQE
                     HHDWQTAGKEYQQRYHRAGSRHKENQEKLQQQIKLRHSLQRQMEEWMGSRDVKQWRDE
                     LERAKDYQSKLGEAHTRLAKIQDGDRELNSAREAMLTLVRQQQEITSVIKNQQILCEK
                     DEREMELLEQQTALENRIRDLETERTRLEEGRPCPLCGATHHPYAQGHLPRLDKTTAA
                     LQQVREEAKQRHQELQALQLRLAGLTKETDLCRMALQKNEAELAQESEDLQQTIRSLP
                     IRMEQPMSLPALQAHINQAEEARVKLAGQIKTIEDLQQQGEQASREYEETLRLCSQSE
                     KELQQAQMQLEQQQKEQERLQREWQRISSELKEMRQACLLEVAEYGIIDLQLPDLSAI
                     LHQLEMRRDRLQEQQAEKQRLQNALDAICNHLMQYTIHLHNQQEIWQNDRQRLAQLGE
                     EHARHRQIRLERYGDRDPDQEEARLEQVIAKAEAETEQTRQAREAIVRDLQRAEERSQ
                     MLADSTRQRADERSSLEHAFVEQLEHAGFPNENAYLQASVLPDELQQLSARAEQLAAE
                     GTEMKTRLAERQENWQREKARQLSDQTAAVLEQEKQQIEQDLQALREQIGANKGILNN
                     NRRLHQQLASQLQQIARQQEELRSWKVLHDLIGSADGKKFRNFAQGLTFEIMTGLANQ
                     QLRRMSDRYLLLRSPEQALGLKVIDNYQGGEIRSTANLSGGESFLVSLALALGLSQMA
                     SHKVSVDSLFLDEGFGALDEEALDTALNTLAGLQHEGKLIGVISHVPALKERISTQIQ
                     VISQAGGRSLLQGPGCRRVVSG"
     gene            complement(18467..19696)
                     /locus_tag="P5549_01725"
     CDS             complement(18467..19696)
                     /locus_tag="P5549_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HRW11823.1"
                     /translation="MKILHTSDWHLGRSLYGRKRYEEFAAFLEWLARLLEEEAIDVLL
                     VAGDIFDNTAPSHRSQEMYYRFLCRVADSSCRHVVITAGNHDSPSFLDAPRELLRHLH
                     IHIIGSIGVDIDSEVLLLQDAQGQAELIVCAVPYLRDRDIRSVDSGETVADKDRKLVE
                     GIRDHYAQVWQRAAAVRDQLERWVPVVAMGHLFAAGGQTVDGDGVRELYVGSLAHVGI
                     DIFPETIDYLALGHLHSAQTVGGQEKFRYAGAPLAMGFGEAGRAKSVVKLEWHNQQMH
                     IKPIEVPVWQELRRVRGDREQIEQQLQGLLANGTEAWLEIVYAGREIISDLREQLETA
                     AAGSGLEILRVFNQHIWDQEMSLSASGELLNELQPREVFQRCLDACEMADEQSDILLK
                     LFDEVINEIDLTDRMAE"
     gene            complement(19747..20958)
                     /locus_tag="P5549_01730"
     CDS             complement(19747..20958)
                     /locus_tag="P5549_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRW11824.1"
                     /translation="MMIRKEMLELLFRPASMQRWNDHIRPHTGFSELDKQSHKMVFAY
                     VLAKTEENDRGIAVDWQRLIEGGIFEFLQRSILTDLKPDVFHRLMEQKSRELNSWVIE
                     QIHDSVASIKGEFLSKLQTYLFETEYAKLEKRILRAAHYLATNWEFAIIDRMNAGFFG
                     LEQTRQNIANEVEEFYDLAGVQKFVLGKKSRNFMDLVGQLRFQQRWGQSPRVPETSVL
                     GHMLIVAILVYFFSAEIGANPKRRYNNFFAGLFHDLPEVLTRDIVSPVKRSVAGLEEL
                     IKTMEEEQFKEKILPLLPRAWHQEMRYFLADEFQSKAKVDGQTLLCSSEMLSEQYAQD
                     EFDPLDGALVRACDQLAAYIEASMSIDYGIKAPDLLKGKEIIRERFSGMKICSIDFKD
                     YYAYWQAKASS"
     gene            complement(21022..22290)
                     /locus_tag="P5549_01735"
     CDS             complement(21022..22290)
                     /locus_tag="P5549_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRW11825.1"
                     /translation="MNRTASTVQITSRLREYVINHPDADRDEVMQAVGIGSIASLYSI
                     LAGLNDDRQICSNGAYAICNNGQRLLLDEIATYLQEKPDRAPSAPKLAERLLYLYWCL
                     HNSIPDGGLSMKAIEKVYLDLFAKSVDHVPAEGALKRMIYRDLETFANLGIWVERSEK
                     SKKYCLQDEYLPKLSSESAAAVYVSMLLYRGTLLDEATLGAKEQLEKSFFKHFPERSR
                     MLKDRIYVMGDTLAHPQEFGNILGKLILAVGESYRVKLTYMTNDGEESARLIEPLGLL
                     CKRNVWYLIARRQNKEMRSYRVDQILHLNVRESERFAYPGDFSIESYLGCSWGVFRND
                     EVQTVKLKFSREVSHRVKNLHYHASQRIFSEEEDGSVILAFEVCGLMEMQSWILQWGT
                     QVEVLEPPALRDSVLQTVRAVALQYEKQDD"
     gene            complement(22647..23075)
                     /locus_tag="P5549_01740"
     CDS             complement(22647..23075)
                     /locus_tag="P5549_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11826.1"
                     /translation="MTEMNTPQAPAPDPEAMKALMAKIAKIQKILKFLYLGQATVAVL
                     LLLLAFMPNLSGSPHVSFAFLMLVPFFCGLAYLASFAGKDLTFAKAAFRNTAISQLGL
                     FIVALYVYVSLGAPMVAVLAVLALIFGALAGMVIYQASKG"
     gene            complement(23182..25209)
                     /locus_tag="P5549_01745"
     CDS             complement(23182..25209)
                     /locus_tag="P5549_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRW11827.1"
                     /translation="MLKRWDTMNDLPLKDTKQLWKAFVERGELPGSNLSPIVKASWQR
                     CYAAGVDPYDGCSHTMLGTAELERLCREHAELIKIALPIMKELNRYVAGSGFLVMLSS
                     ECGYIMEVMGNPETLAQARQINFIKGAAWTEQEVGTNAIGTVLQEKIPLQISSDEHYC
                     QLHHVWTCSAAPIFNDQKQIIGVLNMSGPSPEAHQHTLGMVVSAATAIMFEMRLHAQN
                     QEIIRMSEQMRNVFMSISDGVMIIDQAGRIVQLNPVAENLLGRSSREMENQFCTTVFP
                     ELKKMEEFLNSGSEINELETYLQGFSGTISCILSGQPFRDEEGSVNGGVLIFNPLKKL
                     RSLVNRISGAHASFTFQDIIGQNPKLLEAVQTARLAADKRSNILLQGESGTGKELFAQ
                     AIHNASSRRHGPFVALNCGAIPRELLGSELFGYVDGAFTGARKGGRTGKFELASGGTL
                     FLDEIGEMPLGKQVSLLRAVQEKEITRIGDDKVVPVDVRIICATNKDLRVEVEKGNFR
                     QDLYYRLNVIRIKLPSLRERPEDIPLQFHYFLEQTSMKMEMSMPQVEPAVLDCLCRYD
                     WPGNVRELQNVVERMINISNGREITIKHLPQEIMQDQGRLPEKMAQTVLTGESAGKYR
                     QQQKLLQNQQERQELEQLLEIYGGNISRIAQVKGVSRNTIYRRMRRLGMEL"
     gene            complement(25292..26032)
                     /locus_tag="P5549_01750"
     CDS             complement(25292..26032)
                     /locus_tag="P5549_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRW11828.1"
                     /translation="MKINVNGQQREAHAGESILDLCRREGIPIPTFCYHQAFGGQGAC
                     RMCMVEIKEEGQTRSRMVAACTYPLTQPAEITTESEQILRIRRTIAMLLARRADKNAL
                     LETMCQKYGASRLPAIVVEPANCILCGLCIHACAEMGKSAIWSMFRGVDKRIATPYDE
                     ASDDCMGCAACAQVCPTQAIKVEEEGAVRRIWNKTFALVACTRCGKPYATRDELDYLG
                     QHGDFGQEKLCEACRRKTLAANMQTFQA"
     gene            complement(26044..27861)
                     /locus_tag="P5549_01755"
     CDS             complement(26044..27861)
                     /locus_tag="P5549_01755"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRW11829.1"
                     /translation="MQTEMNPGKGDRQTVLVCCGTGCTANGGREVFKAFSQSLQHHPE
                     IEVLPVVKATGCNGWCEKGPLVRLIPKDITYCSVAPADVDEIISKTLLQGVPVKRLLY
                     RDPVSKEHLTSHHLTDFYRKQHKIALRNIGEIDPGQIDDYIQRGGYQALQKALLGMER
                     QEVIDTIKAAGLRGRGGGGFPTGQKWQACRNAAGNPKYIICNGDEGDPGAFMDRSIME
                     GDPHTVIEGMCLGAYAVGAARGYIYVRDEYDLAVQYLRQAIDDARQRGYLGKNILNSE
                     LSFDIELVRGGGAFVCGEETALLASIEGRVGEPHDKYVFPTEKGLWGKPTVINNVETW
                     ANVPVIMLHGAESFAAIGTDKSKGTKVFSLVGKVVNTGLVEVPMGTTLREIIYEIGGG
                     VLKKRSFKAVQTGGPSGGCIPAALLDLPIDFDSLTEAGSMMGSGGLIVMDDHTCMVEV
                     ARYYVSFLAGESCGKCTPCREGIRALLEILTRICEGQGSIEDLDLLEDISVTMQQASL
                     CALGRTAPNPVLSTLRYFRDEYLEHIQQQRCPAGVCRALSVFHIDPEICNGCGVCVRH
                     CSVNAIRGEKKQVHVIDESICIHCGECYRQCAFDAIKLR"
     gene            complement(27851..28345)
                     /gene="nuoE"
                     /locus_tag="P5549_01760"
     CDS             complement(27851..28345)
                     /gene="nuoE"
                     /locus_tag="P5549_01760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRW11830.1"
                     /translation="MQIDAKEYENASAYQSLIKTYAGKPEYLLAIMQDIQRQYNYLPR
                     LAITMIASHVGLPVSRVYAMASFYKAFSLTPKGRFVIRVCDGTACHIKNSETLLDQLE
                     KQLQVRPGQTTADGLFSVETVNCLGACALAPVMVINGKVYGKVNRSEVERIIKEYGGQ
                     SDAN"
     assembly_gap    28407..28506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28507..30116)
                     /locus_tag="P5549_01765"
     CDS             complement(<28507..30116)
                     /locus_tag="P5549_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRW11831.1"
                     /translation="MFAWQGKILRVNLSDGTIKAEPLNREYAEQYIGARGLGTKYFTE
                     EVDPRVDPLSPENKVIIMTGPLTGTFAGSGGRYNVVTKGPLNGTIAASNSGGTFGPEL
                     KYAGWDGIIFEGRADSPVYLSIYNDQVELKSAAELWGQDVFATTDALKATMDEEAKVC
                     CIGPAGENLVKYACIMNEYHRAAGRSGVGAVMGSKNLKAIVVKGTLGIVVEDPKAFMA
                     AAIDARTKLREHPVSGEGLAAYGTNILVNILNEHGGLPVKNFSEAAVFPNAEKISGEY
                     QTEKYLVRNKGCFGCTIGCGRVTDIPKGPFKSAGEGPEYEATWTFGSNLAVDDFEAIC
                     RANFLCNELGLDPITLGGTIACAIELVEKGIIPRATADLRWGDGAMLVDLVTKAAYRE
                     GFGDELAEGSYRLAEKYGYPEYSMTVKKQEMPAYDPRAQQGIGLNYATSNRGGCHVRG
                     YMTSPEILGIPVKVDPDDTEGKPALLKLFQDLTALVDASGLCLFVTFAIGLPEIAAQL
                     RTATGLDLSDDEYLLIGERIWNLERLFNL"
     gene            complement(30118..30597)
                     /locus_tag="P5549_01770"
     CDS             complement(30118..30597)
                     /locus_tag="P5549_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW11832.1"
                     /translation="MSKVLFVEPEKCTGCRSCELICSYIHAGEFNPLRSRISIIAFEK
                     TGFSFPAVCQQCSVAACMEVCPVGAISVDDVTGAKIVNHAICLRCKMCTIACPFGATF
                     YDADHDLIIKCDLCGGDPECVKHCPSGAISYREAKTANFNKRRANAEKFRDVMEEGR"
     gene            complement(30895..31611)
                     /locus_tag="P5549_01775"
     CDS             complement(30895..31611)
                     /locus_tag="P5549_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRW11833.1"
                     /translation="MHHGQGEPVPERYLRNLQAISASEQAQLRDARVGVCGCGGLGLY
                     VVNYLARLGVGRITVWDPDVFHESNINRQLLASYKTLGQKKAEVCRQYVQEINPEVVL
                     TALPCLWEAGDPVIFAQQQVIVDALDSIPSRLALAEACGRAALPMVHGAVGGWYGQVT
                     VIMPGDKTLHALYSNTDRTGIEEEQGTLSFTAAVIAGMQAAEVIKLLLKRESDLIHEI
                     GMVDLLDLGVERFQKNATPM"
     gene            complement(31613..31837)
                     /locus_tag="P5549_01780"
     CDS             complement(31613..31837)
                     /locus_tag="P5549_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRW11834.1"
                     /translation="MRVRVKLFATFRKGRFKEKEMELDEGCTVGWVIDCLRLPRKELG
                     VVFLNGLSVSDDQQLLAGDTLSIFPMVGGG"
     gene            complement(31855..32766)
                     /locus_tag="P5549_01785"
     CDS             complement(31855..32766)
                     /locus_tag="P5549_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRW11835.1"
                     /translation="MEPVVEPVYNRKHPGTLAYKKFFYKNAIKGVLRPHRVIFKKRRV
                     GRDDHFVLYPQGQKKWYKLFGVPEDVTVPFTYSDFSRAVGFMEVIEGLQSNFKHLLHL
                     KSEIWFYRELIPGEAYIIDYLFEDVLQIKPDKAAMIGCSAISRNGEQYMKMRDHFVIK
                     DLPPRDAVYLQKDTSDQFKGITRVPAEPLDSARMEEIYIPADLAKRYGDTSGDYNIVH
                     TSPLMARLFGYERTFIQGLCSANLIMSRLCMGGIKLRHFTITFGRPVFMDSTVYLYYT
                     DHEYRLQDQDNRVLCFGQINCAEDFSA"
     gene            complement(32898..33821)
                     /locus_tag="P5549_01790"
     CDS             complement(32898..33821)
                     /locus_tag="P5549_01790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW11836.1"
                     /translation="MKGKKQMKVLVADDDKLSRLLVKDLVTSMGYEVLEAADGEQAWQ
                     VLQEHSPRLVILDWIMPRMDGTELCQRLRLLDDENYYYIILLTGLNSKENIIAGLNAG
                     ADDYITKPFIPQELEMRLKAGKRILELQQSLQEALEKQRHMARHDALTGILNRGEIIK
                     MLEKELIRAERQHERLSVIMGDLDHFKQINDTYGHMVGDAVLVETAQRLKDTLRVYDS
                     VGRYGGEEFLLVLPGCALEEAQLLAGRILHAINAQPVRYNNHKIPVTISLGLACNDTP
                     AHAHVEGIVQAADFAMYQAKKNGRNCYAVAE"
     gene            complement(34115..35026)
                     /locus_tag="P5549_01795"
     CDS             complement(34115..35026)
                     /locus_tag="P5549_01795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW11837.1"
                     /translation="MKVLIADDDKISRLVVKNLLNSLGYEVLEAEDGERAWQILQEEA
                     IQLLVLDWVMPKIEGPELCRRLRSSKGEDYYIIVLTGRNSSEDIVAGLQAGADDYITK
                     PFQPQEFAIRLKIARRILELRQTMKEAVEDQRYMAQHDVLTGILNRKEIINVLEKEIH
                     RAQRQKSKLSVLMGDVDNFRQINEMHGKAAGDAVLAEVAKRMKNSLRIYDNVGRYGGD
                     EFLLVMPGCSVNEARLIARRILNVVKNQSVMYYNKDISVTISLGLASHDDPEQADMQS
                     LIQAAIAAMRQAKANGHNCYVVAEPYL"
     gene            complement(35162..35848)
                     /locus_tag="P5549_01800"
     CDS             complement(35162..35848)
                     /locus_tag="P5549_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRW11838.1"
                     /translation="MSNKRPNDSSISGGRPLVYGKKGELAICGRYLVSTEEEIIETRE
                     IIRQINQRYEGWPEHARMKSGEIFPTDVVPVLAWEEDAVHPRLFSWGFPRWQGSGVII
                     NARRETVLEKKMFRQAFLERRCIIPSHGFYEWKKRAGQKQKEKYLLRLPGTPLLYMAG
                     IYTLYRQEGLELPGFVILTTSASPSVQPIHERMPVILRPDEQNNWLRDEIFARRAIER
                     EGPQLEARRA"
     gene            complement(35773..36045)
                     /locus_tag="P5549_01805"
     CDS             complement(35773..36045)
                     /locus_tag="P5549_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11839.1"
                     /translation="MTIMEERRYCNPDKVYVSVKAVFYPDGGFKPTSLIWEDGCEYPI
                     DKVTDIRRAASLKAGGTGIRYTCRISGQTIHLFLEEDRWFMERRGS"
     gene            complement(36055..37317)
                     /locus_tag="P5549_01810"
     CDS             complement(36055..37317)
                     /locus_tag="P5549_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRW11840.1"
                     /translation="MKERVILHSDLNNFYASVECLHRPEIRDKPVAVCGDPLLRHGIV
                     LAKNYPAKATGIQTGEAIWQAQQKCPGLVVLPPNFGLYLRFARLAREIYASYTDQVEP
                     FGLDEAWLDVGGSCQVFGDGREIADRIRTRIKEELGVTASVGVSYNKIFAKLGSDMKK
                     PDATTVITSDNFRQTVWPLPAGDLLYVGRATKRKLASRGVTSIGDIALADPAGLRSLL
                     GKWGEVLWAFANGLDCSPVVRMGEESQIKSVGNSITTPRDLKTDADVEMTFYVLSESV
                     AERMRQHGFKCRTAQIYIRDNNLCSLGAQGKFPRPTQLAADLAAKAMTLFRAHYAWEK
                     PVRSLGVKGTDLVAADCCEQLFLFEDEERRRKQEKIEATVDDLRHRFGHFSIQRALLL
                     QDEKLGAINPKEDHVIHPVAFFKEGRLP"
     gene            37657..38487
                     /locus_tag="P5549_01815"
     CDS             37657..38487
                     /locus_tag="P5549_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW11841.1"
                     /translation="MNRWQQDELDYSGPVQIADNIYWVGFSDEKAGIHCHPYLIMEGS
                     EAVLIDGGSRDDFSTVMLKILRTGADPRNIKRLIYQHYDPDLCGNLPHFEAVINDPEL
                     KIISHRENNLFIKYYSSASPMQCIEELNNSFTFSSGRELKFIRTPFAHAPGSFVTYDS
                     QTRTLFSSDIFGSYDNNWQLYLPCADVCAVCLPQAICPHNGQTCPLHQMLYFHQRIMT
                     SAKALGYALEQIEKLDIELIAPQHGSLIKSKQDCKIIIDQLKRLQPVGIDFYFQEIGQ
                     "
     gene            38484..39743
                     /locus_tag="P5549_01820"
     CDS             38484..39743
                     /locus_tag="P5549_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRW11842.1"
                     /translation="MNTDLTLVQQKLLDAIKTYPNEAALQLLLEDFAARIENKIQNRL
                     MTDLMLKYAELSQKLEATNLFLAQSETRLREAQQIALLGHWELDVATRDLRWSDTMYQ
                     ILQIDPAQKPHYELFMQRVHPEDCDMVAIHYRQLPVSVEPNELRYRLLMPDNQLKWVH
                     IKATAHFDADGKILAARGTIQDITIMKLTEEQLQKFNHHLQELVDEKVQEISESQMAT
                     IFALVKLSESRDDETGNHIERTASLCRLMAERMGLLSRYAGRVNDEFIDNIYHASPLH
                     DIGKVGIPDAILLKPGRLTPEEFEIMKTHTTIGFNTLAQVEQRYTQNTFLQMGMDIAI
                     GHHEKWDGSGYPQGLAGEDIPLAARIMAIVDVYDALRSKRVYKEAFSHEKSRAIIAEG
                     AGSHFDPYLVELFMQIHQEFEIVRDSL"
     gene            complement(39738..40739)
                     /gene="cydB"
                     /locus_tag="P5549_01825"
     CDS             complement(39738..40739)
                     /gene="cydB"
                     /locus_tag="P5549_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRW11843.1"
                     /translation="MGLNELWYSLIGFFFAGFFFFEGFAFGVGMLMQWLGHNEEEKNM
                     IIHSTEPLWGANEVWLLAASGAIFAVFPGWYAVLFNSYGGIFSLILASLLIRGVAISF
                     WHRLHQPAWRKTWNWLLGMTSLLIPFLLAVVLAGCLVGVPINAEKEYAGSLFDLMSLP
                     AMFYGLDLVVFFLYHGAVFLAIKLQGKMRRQARQAARRTGLLSLATAVAVGVVAAITT
                     NMFTSSVAIALTLAGALALLLSYICVCKRLSRLAFVLNEVSIIAIIVALFTALFPRVM
                     VSTVNEAFSLTLYNISASSHILKVMTIAAMAMVPVVILYIVWACSVFKRRLTGGPGL"
     gene            41020..41859
                     /locus_tag="P5549_01830"
     CDS             41020..41859
                     /locus_tag="P5549_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HRW11844.1"
                     /translation="MKTLWKGAVSFGLVNIPVSMYVATENKDLKFHYLHQACMAPINY
                     KKICSKCEQEAKPEEIVRGYEFQKGSYIIMQEEDFARIPQENTKTIDILDFVRLEQVD
                     PIYFDKSYYLEPSAGGDKAYTLIVEAMKKTGKIAIAKIMIRSKQSLAALRVKSEHLVV
                     ETIFYPDEIRSPASLSTGVGTDKLHEKELKMAVDLIENLSVDFDPARYEDEYRQALWE
                     LIEAKIAGQEYVSPTPEPSGGNVVDLMEALKASVQMTESQKTKPVTKKKAAAGGKRAK
                     TGA"
     gene            41862..42791
                     /gene="ligD"
                     /locus_tag="P5549_01835"
     CDS             41862..42791
                     /gene="ligD"
                     /locus_tag="P5549_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRW11845.1"
                     /translation="MTDELGNIRAMEPIRQTAVTDRDDFVYQVKWDGVRIMAGITNGR
                     VCLLNKKGRERTLQYPELQKLPGLLQADRAILDGEIVVMKNDRPHFPAVMQRDRAQLP
                     NTIKLLAQTLPIEYMVFDLLSLNNQDLRKLPLLERVAKLEKVFADQAQLHHVPCFLQG
                     SSLLAAVQEAGMEGIVAKRKNSAYHPARQHNDWFKIKCSQSQKCMVGGYTLHGQKVNS
                     LLLGVYRDDQLCYIGKAANGLREEHWQTLSRELPALETKRSPFAGPVPKEAHFIEPRL
                     AVLVEFLEWTEAMQLRFPVIQAFITANAADCRI"
     gene            42806..43717
                     /gene="ligD"
                     /locus_tag="P5549_01840"
     CDS             42806..43717
                     /gene="ligD"
                     /locus_tag="P5549_01840"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HRW11846.1"
                     /translation="MDKTNTLDIVGHEVSLSNLDRLLWPEAGYRKKDLIAYYGAVYPY
                     MITHLLRRPLVFTRYPGGIDGPSFYQKNVPANLPEWIQTYPWTEANGQTKQYVLVNSA
                     ADLVWLANQACLEIHPWMSQIDRIENPDFIVFDLDPSENNDFTEVVTVARLLKQLLNE
                     LGLRTYPKTSGSLGLHIYLPVVNRYSYEQVRQFGRAIAAIICKVLPDTATIERSVRRR
                     GRKIYIDYLQNGLGKTVCAPYSVRPRPQATVSAPFAWEELDCLAPIEFTLKTMPGLLA
                     RRGDLFSEVLTDRQELEPALKMLGISF"
     gene            complement(43753..>44536)
                     /locus_tag="P5549_01845"
     CDS             complement(43753..>44536)
                     /locus_tag="P5549_01845"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW11847.1"
                     /translation="EALELYYRQFGCGQLGHFSAFRWDNGLQPIAHPDPITWDGIIGY
                     DYQKKVLLDNTRAFMEGRPANNILLYGAKGTGKSSSVKALLHIFAEQGLRMVEITRRQ
                     LADIHEITDQLRYRNYRFILFIDDLSFEDFEVDYKYIKASMEGSLETRPRNILFYVTS
                     NRRHLVRENWSDRQSAGEEVFPPDSQQEKLSFADRFGISLVYLSPDQELYLQMVTEMA
                     EKEGIPMETEELRNQAIQWELSHHGRSGRSARQFIDDLLGRR"
BASE COUNT        10518 a        12046 c        10559 g        11213 t
ORIGIN      
        1 atcttctcgg cagtctcacc gaccgaatct gatactgcga atatgtgtaa tttgtcaggc
       61 attaacacca tcccccttct gtgcgtgcac tattatgtat gtgccacgga acaattctat
      121 actactattt attcgaccat cctgtacata tttccttttt atttagacga aagttcattt
      181 ttgtttttga cagacctgct taatttttac aatccaacaa tctcgcgtaa aattaattac
      241 ccgcgtcatt ttccgtcaca attagcttta ttttgccgga taatatgcta tcatagaaat
      301 agtgaattat gtaacgaata gcgggtgatt tgaatggaag aaaccggtgt ttttattacc
      361 atcctgctgg tcgtcctggg tatcggtatt gtttttgcag tgctggccgt aatcctggtc
      421 aataattcca ccaattggtg gaacaagttg taggtacgtt taaaaacctt tcacgcgcct
      481 gtaggggcag tatgtttctt gttccaacta ccttctgagc ttacaagcct ctcatcttct
      541 cactccgcaa atagcgattg tctctggact ccagcacttt attcaccgtc tccaccatct
      601 gttgtttgtc tttctgcagt gtccctttga tgggcaggta attggaggca tggctgccga
      661 caaaatgcag attgtcgatg gttatgttct caaagatgat tttcatttct tccagggttt
      721 caaaagggtc cagcacttgg aatgtccctt cctgcacctg tcggaacagc ggcgtgcccg
      781 gcacgggcgt gacggtcagc gcagcaaggt actggggatt cattgcattg gtgatgcggg
      841 ctgtcgccag ggcgtgttcg gccgaccggg gacctttacc ggccagtccc agcagcacca
      901 tgctggaaag attgatgccg ctgttgacca gatttcttcc tgcctgcagc atttcgtcat
      961 acgtgacgcc ttttttcact tccttgagca aggcatcgtc gccggtctcg acacctaaat
     1021 aggccttggt caggcctgcc gcccgcaggg cggccagctc tgaaaggctc ttgccaagtg
     1081 tgctttgcgg ccccacatag gagcctacat ggcgcaggcg gggaaacgtt ttatacagct
     1141 ctgccagaat ctccagcaga atgtctgttt caatggcaat ggcatctcca tcacagagaa
     1201 acactttttc tgcatcccca taataatcct tggccatttt gatatcggtt ttgatttcct
     1261 ccaggctgcg tatccggtat tttttgtcct tgtacattcc gcagaaggtg cagcggttgt
     1321 gcgaacagcc gatggtgcat tgcagcagat agctgttggc ttcgctgaac ggcctgaata
     1381 tattgccttc atatctcatg cttgtctgtc tccttgatca ttgttttctt tactgtatct
     1441 ccaacatttc tatggcgtca tttgctccgt tgtccgtctg gttgccgacg tctggcaacg
     1501 gcaaatcttc gggttgttca ttgtcattgc tgtccggtcg atccggattg gtcgcatcac
     1561 cgggggattc tggttggggg gattctggtt ggaggttttc tgcatccggc ggcaattctt
     1621 ccagcggctc ttcttccgtt ggtgtttcct cctgtaccgg cgcctgttca agcggttcct
     1681 gattggaggg aacgctcagc actccctggg aaatgagccg gttcataaaa tcacgccaca
     1741 ccagtgcgga cgtaccgcca ccgctgaccc ggcttccgcc tacgggttgg ttattactgt
     1801 tgcccaccca gacagccgtg gacagctgag aagtataacc cataaaccat agatcacggc
     1861 tttgggtggt ggttccggtc ttgccgccgc tgcggatgga aatttgggca ggtgtgccgg
     1921 tgccgtaagc cacaactgat ttcaggatgc tgtccatggt agcagcggta ctgctgtcaa
     1981 cgactttctt ggcactgcct cgtccggcat acagggtttc tccgttgttg ccgacgagcc
     2041 gttcgatgct gtaacaagga gatattgtgc ctccattggc gaacatggta taggcactgg
     2101 ccatttccat aggagatatg ccattgctca ttccgcccaa tcccagggcc aggttgtggt
     2161 cttctttggt gatggaggtg atcccgtatt tctcaagata tttaatggcc ttgtttacgc
     2221 ccagcatatc cagcacttcc accgaggcca cgttgtatga attgacaatc gccagccgta
     2281 cggtggtttc ctccgggctg acggcatagt tgttgggcgt ataaccgtta aagctgcgcg
     2341 gtttgttgtt cagtttggta tccgcctgga ttacgccttc attgatcgct gctgcataat
     2401 agagtggctt gatggctgag cctggctgac gcggttccac cgccatgttg atctgatttt
     2461 tgtcccattc cacgcctcct accatggccc gcacggctcc cgtctgaggt tcgatcgcca
     2521 ccagagcgac atcctgggcg gtaatccccc gggcagcaaa ggttgccgcc tgtttgacga
     2581 ctgcatattc agcggccttt tgcatgcggt tgtcaatggt ggtataaatt ttcaagccgc
     2641 cctggtagag tttctgggcg cccaattctt tttccagctg tctggccaaa taggtcatat
     2701 agtagggatg ggcactcaag ctctgacgga acggttcaaa agcaagctct tcctgaaaaa
     2761 tggcttgccc ttcctgcgcc gtcagcaatc cttgttcctc caggagattg accacggttt
     2821 gctggcgttt ttttaatcct tccagattct tgccggggga ataatactct ggtgattgaa
     2881 tgattcccac caaggctgtg atctcagctt tgctcagatc gaagacatcc ttgccaaagt
     2941 aagcccgggc agcgatcccc atcccggtgc acccgcgtcc catatatatt tcattgagat
     3001 acatattcag gatggcatcc ttggtgtact tgtcttcaat ggcgctggcg ataaaaactt
     3061 ccttgatctt gcgccaatag gtcttttcct ggcttaggaa gagggtccgg gccaattgct
     3121 gggtaatggt gctgccgccc tcggccttgt tgcctgatct gatgttgaca tagatggccc
     3181 ggccgatact gcgggaatca aagccggaat gctcataaaa acgtttgtcc tcaaccgcca
     3241 ccaccgcatc tttcatgaat ttggggaact cttcgctata ctcacgctga taacccagat
     3301 tgcccatttt ctttccgtca tgataaaaaa cggcggtttg tccttgtacc tcatactccc
     3361 agttgcttat gcctgtcaca aatttggcca gcttgggcgt ggtctgggtc agcgacaccc
     3421 cggtgtagaa aaacatcagg aataataaaa tcgcgcccgc tgccagcaaa agtttgctta
     3481 tgaacttcat tgcatcaaga cctttctgtc aattagactt tcccgttctc ttcccgggga
     3541 acagtcctgg tcgtaaagga tccctattca atttgctttc ttcataatta tatacagttt
     3601 atacgctgaa agctcacatt tcattatcgt ctcagaataa aaatatttca acattctgtc
     3661 tatcatcaga aatagaagtt ccttcagcag gaaatttgct ataaaatgat gaagatacta
     3721 agtgttgggt aaacattttt ccgatcacct tctgccaagc ggcaatgaac aaagagcgat
     3781 atgaggagga ccatggaatg ggcaatattt tagaggaatg tcaggtctac gtagtaacat
     3841 ccggcaaagg cggagttggc aagacaacca ccgtagccaa cctgagcacc ggtttggcca
     3901 aactaggtta taaagtcgtc gtcatggatc tggatatcgg tttaaagaaa ctcgatttga
     3961 tcatgggatt ggaaaatcgg gtaatctacg atatcgtgca ggtggcggaa ggagagtgta
     4021 cgctcaaaca agctttggtg aaggacaagc gtttccctga tctttttatg ctgcccgctg
     4081 cccagaccag aaacaaagag gatattacaa tcgaacaggt caaagccatc tgtgatgaac
     4141 ttcgtgaaga atttgatttt atttttattg acagcccggc cggcatcgaa cagggtttta
     4201 agaattcgat tgcagcagca gacaaagcaa tcgtggtcac caatccggaa gtatccgccg
     4261 tgcgggatgc agatcgcatc attggcatgc tggaatcggc cggtttcaaa gacatccagc
     4321 tgctgatcaa ccgtttgcgt ccggatatgg tcaaaaaggg agacatgttg agtattgaag
     4381 atctgatcga acatctctgt atcgaaccat tgggcgttat cccggaagat aaaagcgtcg
     4441 ttatttccac caatcggggc gagccaatcg tcctgggcaa cactccggca gccaatgctt
     4501 tcagcaacat cgtacagcgt ttgactggtg aagatgtgga aaccatggta atcccggaag
     4561 atacattttg gaccaaagtc aagcgcaact tgtttggcgc cagctagagg agggttttat
     4621 catgatggaa ttactgagaa aattatttct gggagagcag aataccagcc gcaaacaagc
     4681 caatgaccgt ctgcgggtgg tgctgaccca tgaccgtctg acggtgaatg ctgatttgat
     4741 ggagaccatc aaagaagaaa tcctgcaggt catcgccaaa cacattgata ttgagggaag
     4801 tccggaagtc aatctgataa ccgaagggcg tcatgctgcg ctggacatca gcataccact
     4861 taaaggaagg tagatgagat ttggccaaac ttgacgttaa gggaaagaat aataatctga
     4921 tttttgtttt tggagcaggc agttttgagg aactggccgg attgctcaaa gaacgggttg
     4981 agaacaatca ggcacttttc agcggctccc aggtcctttt tcagggagac ggcttgaaaa
     5041 ccctccgtct ggaagaactg attgctttac aaaggatgtg cctcgattat ggcatggtct
     5101 taaacaatat gcccgcacca cagccggcgg cagcaccagt gtcggccccg gcagccgctc
     5161 ctgcgccggc agccgcccag ccggaccgga aaacacacgc aaccggtcgt gatctgatga
     5221 ttttcaagac cctgcgcagc ggacaaaagg tacacagtga aggttccatc attgtctggg
     5281 gcgatgtaca tgaaagtgcg gaaatcactg cagccagtga cgttgtggtg cttggccgtc
     5341 tggaaggact ggcgcatgct ggctgcttcg gtgacgtcag cagcaccatt tttgccctga
     5401 ccctgacccc gcgtcaattg cgtatcgctg atcgcatttc tcgttcttcc ggtaaaatcg
     5461 tcaggaatcc ttacccggaa attgcctacg tggagggcga tggaatctgc atcagggaat
     5521 actccaaccg tgacaactta aacaaatagg caaagcttca tctttgcctg tttcttttcc
     5581 gggagggtga agggatgtca aaaagaaata atgcccccgc cgccacggct caggaattca
     5641 tctccagtca accggtgacc ggctggaaac agtggcggct tccttttttt attctgcttt
     5701 tactgcaggc agtagtactg ttgggcgtcc tggaaagacc ggaaatgtct ttgtatgatg
     5761 cctggtttcg cctgcaaggc gccgctgatc cgggacagca agtggtcgtg gcggccatgg
     5821 acgaaagctc aatcgaccga ctcgggccgc cggcctggcc tcgttcagtt catgcccagt
     5881 tgttggagaa gttagtctcc gccaaaattg tggcctttga tcttacgttt ggcgccgcca
     5941 aagacgctga acaggatctg gctttcgccg aggccattgc ccaacacggc aacgtcgttc
     6001 tgatcagcaa gttctatttt gaaaaagatg aagacggtga tgtcgttcag atccctgaac
     6061 tgcccctgga ggaattggcc gcctctgctg ccgggatggg ctttgccaat atgcccaccg
     6121 atcctgatca ggtcgtgcgt cacacaacgc tggttgatac caatagtttt gatattccct
     6181 atccttcctt tgctacggcc atcatcctgg ctgcccagga tctgaattac gaagatctgg
     6241 agctggggga tggatacctg caggccggcc agtacaaggt tccgctggac agtcgtcatc
     6301 aggccatgac caccttttgg ggaccgcgag gaacctttca aaccatcagt tatgcggata
     6361 ttttggagga aaaaatcagt cctgcctatt tccaggataa aatcgtgctg gtgggcagta
     6421 ccacccagga tgaacatgac tattttgcta cgccctacac taccagcaac atggtaaaaa
     6481 gcggtgccct ggaaacacct ggagtggaag tccacgccgc tgtagtgcag agttatctta
     6541 atcaaagctg gtaccagcgc gtcggtcggg ggttgaattt tctctttctg ctcctgctta
     6601 ccctgctgac cgtcaaactg gtggcaggac ggggccctct ggctggtttg ctgggtgtgc
     6661 tgggcgtttc ggctctaacg ctgctgacgg tctttgtgct gtggaagaat cactggtggc
     6721 ttgatttggc ctctccgctg gcattgatct ttctgcttta tgttgtcgtt actgccagtg
     6781 actttgtgcg ggcggaaatg cagcggcgca agacgaaagc cttgttttca cgctatgtgt
     6841 cagccgatgt cgtggaacag ctgatggcag atcctgccca aatggcactg ggcggaaaga
     6901 agcagctggt cacggtaatg tttgccgaca ttcgcggttt tacggctttt agtgaaaaca
     6961 aggaccccgt cgaggtgatc catcgtttga atgaatattt gaccgtcatg acggaatcca
     7021 tcctaaaaca tggcggcacc ctggacaaat atctagggga tgggctgatg gcctttttcg
     7081 gtgcgcccat ttactatccc gatcatgtgg aaagatccat cctggtagcc cgggagatcc
     7141 aacagaaagt agctgaacta aatcgccagt gggagaccaa aggggaagtc cccctgctgg
     7201 ttgccgtagg aatcaacacc ggcccggcag tggtgggcaa cgtaggcagt cctgagcgga
     7261 tggattatac tttgattgga gaagatgtca atctggcttc ccgggtggaa gctttgacca
     7321 aactgttctc gaccctggtc ctggtttcgg aacgctccta tcacctgctg gcagatggtc
     7381 cggtcaagga ttcactggct tacgtaggcg aagaactggt aaaagggttc acccatccga
     7441 tcaaggtata tacctttacc gatctggatc tgcattttga gaagtccacc gacagtggtt
     7501 ttaaataagc agttccctca tcatctgcaa cttgggcagg caatcctggc aatatttcca
     7561 tctttcccct gcgtttgccc gatcatcctc tgatccacgc caaaaggctt ccggcaaaac
     7621 cggaagcctt ttttaattct gaacctgtca tcctcgttgg ccaagttatt ttaaatcaga
     7681 agtgcaatag gggcagcgcg tcgccgcaat cgcaactgac gatttgcaga acgggcaatc
     7741 cttcttgtcg ggttccgcag gggcttcctg cttcttcatc ttgttgatct gtttgactac
     7801 caggaaaatc acaaaagcaa caatgagaaa atcgataata ctgttgataa aaacaccata
     7861 tttgattacc ggagcaccgg cttcaacagc ttcggccaaa gaagcaaact cctgtccgga
     7921 caggtttaca tacaggttgg caaagtcaac tttgcctaac agcaagccga tgggcggcat
     7981 aatgatatca ttgacaaaag aggtgacaat tttcccaaat gctgcaccga taatgatacc
     8041 tattgccagg tcaattacat tgcctttcat ggcaaactca cgaaattctt tccacatctt
     8101 atctgccccc ttttcttatt tcagcccaaa tcaggccacc gtgtaatcct tcacctcctc
     8161 catgctgcga ttacatccta ttcatgcccc taaagacaaa tgattacacc agcggagaca
     8221 ggagcgtgac cagcgggatt ctcctctgcc cgccggcttg gttcacggta aagctaacga
     8281 aaagatcgct ttccgagatt tcgggtacgt tgagttcaac cttggcaaca aagtccccgt
     8341 caatatattc acatacagcc gttcccagca gattgccgcc ctccgccgca tcatagacgt
     8401 tgaccccggc accctccggc agactgaaaa atattatatc cagactccca aaactgttct
     8461 cccggctgaa taccgacaga atatccgccc aggaatcgtc ataatcaatg ggcggcaggc
     8521 tggaaccata ggcattgtct gcaatcatat cctggaccgt gttgacctgc ggattcagga
     8581 tatcaaacca caaaccctct ccggtcttca tgtaaatccg gttggaaccg tgataataca
     8641 attcccagat aaattcctca ttgccaaaat acaggctatt caagccataa gcgccatcgg
     8701 cgaccaggac tgcgggagcg gtgacatcat acggggaata ttcaccttca gacgaccagt
     8761 agtccagggc aagatcgaat tccaagtctc cattgctgac gtccagggtc atggaatcca
     8821 tgtagctgta gtctgttgat agccacagga taaagcttga gggggggagc accaccgcat
     8881 attcaagcga ctggcttccc gccggcagga aatattcagc atagctgtaa aagccatcgt
     8941 tggagagatc caaataaacc gttgtatcct gcggtgcagc ctcttcgtct gtcaaataaa
     9001 ttgagcccga taagagattc ccggtactca ttgtccaatc catccctgct gcaggctcgg
     9061 cggcgactgt caccacgact gcatcttctg cattcaggct gctgccttca gtggtgtaat
     9121 aaccttcccg cagcagatga ctgttgaccg gctctgccgg gttgacggga ttgccgaggt
     9181 gataatacaa acagtaactg gcctctgcgg gaaccgtgat cgcaaaagca gtgctgttct
     9241 ggccgctggc caggggaacg aaagcatagt aatattcctg attgtccgcg gaaatcatct
     9301 cggcctgtat gatgatatcc agatcgcttt ctgccgcacc gccttcaggc aagcgaatcg
     9361 tgccggaaac aatctgaccc gtttcaatat gcaaatcgat gttttcttga ctgccctcgg
     9421 ccacatcgat catctccgag tattggcccg acataaccag gttttcatca taactgccga
     9481 ttagatcgaa atacaggttg taagctgccc tactgtaagt cataggctgc atgccatgat
     9541 gatgaggaat cctgtccgca tgacgatagg cacccgcaga tgcttccggc tcggacagcg
     9601 cctggaaccc ttcgggcata aagccgggca ttaggtgggc ttcgtccaaa accgcaatcg
     9661 agtactcggc ctgcgactgt ccttgcgcaa tggtcaccgg tgcagtatat gcacaaaact
     9721 gaccgtacaa atacaacata atgtccatac cttcagccgg agccaccatc ccttcaggca
     9781 aggcaatggt accggagatg gtccgatctg ccaaattgac gagtgttccg gaaccatcca
     9841 aaccggcgtg ggcaacaata tactcattct cgtcgaaaaa taccacctgc aatctttccg
     9901 gatcggcaaa cagtttcttc atgacccgtg cggacgcatc gaggctatgc ttttccacca
     9961 cgggtgtcgt tgccatccag atttcataag aaccggcccg ggcatacagg gaggcagccg
    10021 gattcatgct gaaatcctgc gttaaaacgc catcgtcata gatatagtca tccaaaacca
    10081 gccgatcggt cagggcataa ctccccagga tttctcccaa tgcggcagct gtaacgctga
    10141 ccggcagctg tactgcaccg ctaaagccgc tgaccgttcc agaaagagtt tgcacaccaa
    10201 cccgggaagt atccactgca gtggggctcc aggtgacact tacattgcgg ctggaaccgt
    10261 cactataata ggctgtcact tcggtcggca atggataact ctgcccggct tcaaccgtca
    10321 acgagatatc actgggcaat tgcagtcggg taataacagg caggattgtt ccgccgccgc
    10381 ccgagcttcc cccgccggag ttctgccgga ttaatctctg ggcctcctgc aaggcccggt
    10441 ttatggcatc cggcaaatca gattgttgca atacagaggg tggatttaaa atattgtcca
    10501 ccatgtcttt ctcatttaat tgctgattta tttgggtctg ttgggcccgc tccagcagcc
    10561 attccttcac ttcaacccat tgccgcaatt ggttttggtt catcggccgg ggcggcgctg
    10621 taaactgacc cgagctgccg acttcaacaa attgtcccgc cccaatttga tagctgttct
    10681 gaccttgctg gagattatgg ctgtcggcgg tcacgctgcc gtccaaaaca gtaatatgcg
    10741 accttccggc agcactctgg gcgatataaa actgggtccc gcgtacggcg gccacgcccc
    10801 agggcatgtc tacttgaact ttgacccgct tggttttgga ggttttgtac cagggagtgg
    10861 ctttcttgct cttggcagcg gccagcaatg cgggagggtt taacccggcc agcagagaac
    10921 ggttgaattg ctgtaaataa ccggtattct caggttcaat actttcttcg atgttttctt
    10981 gctcctcctc cgaggtggcg ggcacagtcc caaagacttc acccgtggtc atgtctatct
    11041 tcagccattc caccgcgacc gccgcactgc cgtcctgttt aatataggcc cgtccgtgtg
    11101 attcaataat cgacagacga gtattggcct ttagcaataa cccgctgcca tcctcaaaac
    11161 tgatttccgc ttccgtgttg ccggcggtta caatcgtatc accgattcca actgtcgttg
    11221 gctgggttac tttgaccggt tcctgttggt ctttctgtag ggtaaccgta ccttttgtca
    11281 ctgccaacac accgaccagg tcagttttat acaactgcgg atcttccgtc atcagaacgg
    11341 ccaaggcacg tgccattgcg gcccgggtca tggttttttc aggctgaaac tgactgccgt
    11401 cgctgccggc cagcatcatg cccgcttcga ctgcagtggt tgctgaagct gctgcccaat
    11461 gtccgctgtt catatcgcta aactgagctt cgtcagcatt gtcgagactt tgacgggata
    11521 atcgcaaaac cagcgtggct gcttgtgcgc gggtcagttg ttcttccgga tggtaacgtt
    11581 tgtcaggaaa acctttgatg taacccgctt gaaccgctgc ggcgatactg gctctggccc
    11641 agtgatcggc tggcacatcg gtaaagctga ctgacagagt gacatccgga gtaattcctg
    11701 cggctctgat caacaagcaa gctgcctggg cacgggtaag cccctcgtcg ggacgatagg
    11761 ttccatcgtc aaatcccttg atgatcgact ggctggccag atactcaatg taggctgcct
    11821 caggatgatc cgttaaatct tcaaatgact gtacctgcag gccgctcccg gtctttattt
    11881 catttcccca ggccggcaac acgataacga aaaccatcag gaacaccagc aataagaaaa
    11941 ctcctgtccg cttctctgct ctcctgctca ttgccactct ctccttcttc atttatcaat
    12001 aagtaacgtt aatcatttga catgttactt tatttttcct gctggatttg actctgctga
    12061 aaaatttcag gtgatccatg ccaagttctt ttcctcaaaa ttccatattt gattaatcgt
    12121 catcgattgt gcaaaaccgg ggttgattat gtcacattat ttcaattgca atcgcttgcc
    12181 aaaacagtta ttttttatat acaggctgaa ttttcataaa tttttgtcaa atccctgccn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tcaggtaata tcaggtatta
    12361 tgtgcagcat tatgtattct tgtgttatga ttaaatggat atgtaataca tttacatata
    12421 gttggtcgat acaatcattt aaggaggtac tgaaatggcg gaaaattata aggggatcca
    12481 gtacgaaacg attctggcct gggtggaaga cggcatcggt tacctgcagt tcaatcgtcc
    12541 caaagcattt aatgcagtca actacaaatt attgatcgaa gcgaccgaag ctctgaactt
    12601 ctacagtgaa gatcctgatg tcaaggtgat tatcgtctgc ggtaatgaaa atgcattcgc
    12661 agccggggct gatctcaaat ttgtttccgg ttttaatgct tttgaagccc gcgattttct
    12721 ggacaaagtg catgattgta tgtttgccct ggaagacaat cacaaaccca ctattgctgc
    12781 cgtacgcggt ctggctctgg gcggcggact ggaaaccgtc atggcctgtg atattcatat
    12841 tatggccgac aactgcaccc tcggcctgcc ggaaatttcc ctgggaatct tccccggcgc
    12901 cggcggcacc cagcgcatgc cccgcatcac ttcactgaat attgccaaac agtatatcat
    12961 gaccggcgac ttctttgatg ccgcgactgc ctaccgtatt ggcctggcca atattgttgt
    13021 accggcagat gaagtgatgg gcgaagcctt gaaattcgcc aagaaactga ccaagaagag
    13081 tccgctggca cttcgcgaag ccaaaaatgc catcaacaat tccatgaact acgacatcaa
    13141 ggccggttgc cgggctgaac aaattgcctg gtccatgctg ttcagttccg aggatcaaaa
    13201 ggaaggcatg gcggctttcc tggaaggcag aaaggcccag tttaaaggaa aataacccgg
    13261 accgacagaa acggcctgac tgcatagcca ggccgttttt tttatgagac ttcattcgat
    13321 gtaagacttt atttttggct gggcaatgat ttcgctacga agcgattgcg gcccgccttt
    13381 ttggcttgat aaagcagatc gtcggcacac tgtacgaact tatcccggct ctcgccttga
    13441 tagtcgcaaa ccccgatact tatggtgatt ttgatctgat cctcgccaaa gcttgcttgc
    13501 gctacagcgt tgataatcct ttgggcaacc acctcggcgg cttgcaaatc cgtgtgggtt
    13561 agaatcaaaa ggaattcctc ccccccgtaa cggcccagtc ggtcggtttc ccgaatgttg
    13621 ctcaacagta tccgggctgt cttttgcagc ataaaatccc caaactgatg tccataatta
    13681 tcattcactg ccttaaactt atccagatcc agcatcatca agctgagcgg atatccatag
    13741 cgcttgctgc gaatgatttc cttgtttaaa attgcgaata tatggcgtcg gttatacagt
    13801 ccggttaaat cgtcgcgggt ggcttgccgg ctgagcaatt ggttcaactc ttccaactgc
    13861 tggtttttct ctgccagttc tttttcaatg cggcgaaact cagtgatatc atgtaccatc
    13921 gaaaaagcaa attgctggtt attaatggca atgatttcgc tggtcaataa cccatgaatt
    13981 ttttcaccct gccggttgta tgtaattaat tcaagattgt caattctttt gtcccgggcg
    14041 attaatagtt taatgagatg cttatcgtcg gaattcgcaa aaagattgat gtcaagccaa
    14101 ctgcggccga tgatttcctc cctggacaat ccaattgtgg aacacaacct ctcatttaca
    14161 tcaacgtaaa tgccgtcttc atcttcttta tacgtagtga tgctctgcaa ggcgttggca
    14221 ctgttaaaaa ccagttcaaa tttctgctgg tagctgtgca ttgccgttac atcataaaaa
    14281 acgctgacca aatggtcttt gtcgagcttg tgcaggaatg attgatagca ggagttgcca
    14341 agcaaaagca acggcgtggg ggggctgact tcatcagatt ggcttagcca tgctgccaga
    14401 tcaaagcccg ggaataatcc gctgaaatga tctctgccca gacaacggcc cagcaattgg
    14461 gaacggtcgt gaccgcttag gctttcgaaa gtgtgattga cggctacaaa ttgataatct
    14521 ctttgctctt cccggagtag catctgataa caagcaacgg cacactcaac cacatccagc
    14581 attttttcaa ggatgatctc tccagtcaat cctgtcacct cctcctgccc agcattgaat
    14641 tccaatcatt atcatccagt tcggtaataa caaacaatcc cgtatgataa atgatatcac
    14701 ttcgcctcct ccccagcaac gcctgcttta tatatgtctt accaatccca accgctcccg
    14761 gcagaatcac ttttaaccgg aaacaacccg acggcaaccc ggtccctgca ggaggcttct
    14821 gcccccagcc tggctgatca cctggatctg ggtgctgatc cgttctttca gtgccggcac
    14881 atgggagatg actccgatca gcttgccttc atgctgcagg cctgccaggg tattgagcgc
    14941 agtatcgagc gcttcttcat cgagtgcacc aaatccttcg tccagaaaaa gggagtcgac
    15001 actgacttta tgactggcca tctgcgacag ccccagggcc agggccaggc tgaccaggaa
    15061 gctctctcct ccagaaagat tggccgtaga acgaatttcc ccgccctgat aattgtcaat
    15121 caccttcaag cccaaagcct gttcagggct gcgcagcagc aaataacgat cgctcatcct
    15181 gcgcagctgt tgattggcca gtccggtcat gatttcgaac gtcaagccct gggcaaagtt
    15241 gcgaaacttc tttccgtctg cggagccaat cagatcatgc agtaccttcc agctgcgcaa
    15301 ctcttcctgt tgcctggcga tctgctgtaa ttgggatgcc agctgctgat gcagcctgcg
    15361 gttattgttt aaaatgcctt tattggctcc gatctgctcc cgtaaggcct gcagatcctg
    15421 ttcaatttgt tgcttttcct gctccagtac ggcagcagtc tggtcactca gttggcgggc
    15481 tttttctctt tgccaatttt cctggcgctc cgccaggcgg gttttcatct cggtgccctc
    15541 agccgccagc tgttctgccc gggcagacag ttgctgcagc tcgtccggca gcacactggc
    15601 ctgcaaatag gcgttttcat tggggaaacc ggcgtgttca agctgttcga caaaggcgtg
    15661 ttccaaactg cttcgttcat cagcccgctg gcgcgtggaa tcagccagca tctgactgcg
    15721 ttcttccgcc cgctgcaaat cgcgcacaat cgcctcccgg gcttgacggg tttgttccgt
    15781 ctctgcttca gccttcgcta ttacctgctc caaccgggct tcttcctgat cgggatcacg
    15841 atcgccatac cgttccaagc gaatttgccg gtgccgggca tgctcctccc ctagttgtgc
    15901 caagcgctga cgatcattct gccagatttc ttgctggttg tgcagatgaa tcgtgtattg
    15961 catgagatgg ttgcagatcg catccaaggc gttttgcagg cgctgctttt cagcctgctg
    16021 ctcctgtaag cgatcccggc gcatttccaa ttggtgcagg atggcggaca agtccggcag
    16081 ctgcaggtca atgatgccgt attcagccac ctccagcaga cacgcttgcc gcatctcctt
    16141 taattcactg ctgatccgtt gccactctcg ctgcagacgc tcctgctcct tttgctgctg
    16201 ctccaattgc atttgcgcct gttgcagctc tttttctgat tggctgcata ggcgcaatgt
    16261 ctcttcgtac tctcgactcg cctgctcgcc ttgctgttgc agatcctcga tggttttaat
    16321 ctgcccggcc agtttaaccc gggcctcttc cgcctgattt atatgggcct gcaatgctgg
    16381 caaagacatg ggttgttcca tcctgattgg caatgaacga atggtttgct gcaaatcctc
    16441 gctctcttgc gccagttcgg cttcattttt ttgcagcgcc atccggcaca ggtcagtttc
    16501 cttggtcaat ccggccaatc tcagctgcag cgcctgcagc tcctggtgac gctgtttggc
    16561 ttcttcccgg acttgctgaa gggctgccgt cgttttgtcg agacggggga gatgtccctg
    16621 cgcatagggg tgatgggtcg ctccgcacaa cgggcaggga cggccctctt cgagtctggt
    16681 tctttctgtc tccagatcac ggatacggtt ctccagcgcg gtttgttgtt ccaaaagttc
    16741 catttcccgc tcatctttct cacaaaggat ttgttggttt ttaatgaccg aagtgatttc
    16801 ctgctgctgc cgaaccaacg ttagcattgc ttcccgggca gaattcagtt ctctatcgcc
    16861 gtcctgaatt tttgccagtc gggtatgcgc ctcccccagc ttgctttgat aatctttggc
    16921 acgctcgagt tcatcacgcc attgttttac atctcgactg cccatccatt cttccatttg
    16981 tctctgcaag gaatgccgca gtttgatctg ctgctgcagc ttttcctgat tttctttatg
    17041 acgggaaccg gcccggtggt atcgttgctg atattctttc ccggcagtct gccagtcatg
    17101 atgttcctgc tgtttttgct gcaaaacttc gcccttgtct cgaagttgtt caaaaatccg
    17161 gcggattccg ctgaatttag tcaggagtgt ctcgtctcgt tggtttttct gtaaatattc
    17221 cgtcgccgct gcaatattcg cagccatctg ctgctgttgc acttcagccg cggtaatttt
    17281 tccctgccat tcctgacagg ttgcctgggt cgtcgcaacc atcgttttca attgttccat
    17341 ctgtccggcc agttgtctga tcatcagatc catctcccga acctgacggg cggtttcgct
    17401 ttcgatttta cgggcggctt tggcttcatt cagtctttgt ccggcatccc gccacgcctg
    17461 gtcggcgctt ttttgcgctg ccctttgtgc cggaatcact gcagccagct ggttcagttc
    17521 agcaagttct tcagcctgcc gcctccttac caacatcaat cgatggtagg ctccgtccag
    17581 tcccaccgcc tgccgggcca ggcgcagcct ttcctcgtca ggacgaaaag cttcctgccg
    17641 cgccaaaaaa gccttccact gcagcttgac tgccttgact tcttgttctc gttcctccaa
    17701 ccgggtcagc caggccaacg cttcggtcaa ggtttgcagc tgggcttgac cggctgtatc
    17761 ctgctcattt aacgttttta gcagtatttc catttcctgt tcttccgctt cggttaatat
    17821 tttaatattg ctggtggctg cctgcagcag ttccaattcc tttttcttgt cactgtaccg
    17881 ctgatgtacc cgaatggaga tctcgctgta gattgccgtc ccggtaatct gctccaggat
    17941 gggtgccctt tcatccgctg gtgcctgcag aaaggcagca aatcccccct gggccagcat
    18001 catggagcga gtaaactgaa taaaactcat gccggtgatc tgctccaccg ctgcagctac
    18061 atcacggagc cggttttcca ggatctgacc gctgcgcagg tcggcaatct catgcttggg
    18121 aggttgcagc tctccttccg cttttttgcg ggcgcgctgc tgactccaat gacagcaata
    18181 ctctccactg ccggttgcga atacgatttc agcgaaacag ctgccagtat gacgggacat
    18241 aatttcattg ctgcttttat tgatccgttc caaacgcggg gttcgtccgt acaaagccag
    18301 acatatggca tccaggatgg tggttttgcc tgccccggtg ggaccggtta tggcaaatat
    18361 tccattgttg cggtaggccg gttcggtaaa atctatttcc caaacgcccg tcagcgaatt
    18421 gaggttttga aagcgaagtt tctctatgcg caaatcaatg tcccccttat tcagccattc
    18481 gatccgtcaa atcaatttca tttatcactt cgtcaaatag cttcaacagt atgtcgcttt
    18541 gttcatcggc catttcacaa gcgtccaggc agcgctgaaa aacttcccgg ggctggagtt
    18601 cgttcagcag ttctcctgaa gcggacaggc tcatttcctg gtcccaaatg tgctggttga
    18661 aaacccgcaa tatttcaagc ccactgccgg cagcagcagt ttccagctgc tcccgcagat
    18721 cactgatgat ttcccgtcct gcatagacga tttccagcca ggcctcggtc ccgttggcaa
    18781 gcagaccctg caactgctgc tcaatctgtt cccggtcgcc gcgaacccga cggagttcct
    18841 gccaaaccgg cacctcaatt ggtttgatgt gcatctgctg attgtgccat tccagtttca
    18901 ccactgactt ggcgcgtccg gcctcgccaa agcccatcgc caagggcgcg ccggcatagc
    18961 ggaatttttc ctgtccacct actgtctggg cactgtgcag gtgccccagc gccagataat
    19021 caatcgtctc cggaaatata tctataccga catgcgccag cgaaccgaca tacaattccc
    19081 tgacgccatc tccgtctact gtttgtccgc cggcagcaaa gagatgaccc atggcaacaa
    19141 ccggcaccca gcgctccaac tggtctctta ctgctgccgc ccgctgccat acttgggcat
    19201 aatggtctcg aatcccctcc accagtttgc ggtccttgtc tgccaccgtc tcaccactgt
    19261 ccacactgcg aatgtcgcgg tcccgcaaat agggaacggc acaaacaatc aattccgcct
    19321 gcccctgagc atcttgcagc agcagcactt cactgtcgat atcaacgccg atgctgccga
    19381 tgatatgaat gtgcagatga cgcagcagtt cacggggagc atccaggaaa gagggagagt
    19441 cgtgattgcc ggcggtgatc acgacatgac ggcaggaaga atcggccacc cggcacagaa
    19501 aacggtaata catctcctgg gagcggtggc tgggtgcggt gttatcgaag atatcacctg
    19561 ccaccagcag aacgtcaatc gcttcctctt ccagtaaccg ggccagccat tccagaaagg
    19621 cggcaaattc ttcataacgt ttgcggccat acagggagcg tcccagatgc caatcagagg
    19681 tatggagtat tttcataagg tcttccgtcc tttccggttt cagttggcat aatgtcaggc
    19741 tgcggatcag cttgaagctt tggcctgcca gtaagcgtag taatccttaa aatcaatcga
    19801 gcagatcttc atcccggaaa agcgctcacg gattatttct ttgcctttca gcaaatccgg
    19861 tgccttgatg ccataatcga tggacatgga ggcttcaatg taggcggcca gttgatcaca
    19921 ggcgcgaacc aaagcaccgt caaggggatc gaactcatcc tgcgcgtatt gttcgctcag
    19981 catttctgac gagcaaagca aggtttggcc atcgaccttg gctttgctct ggaattcatc
    20041 cgccagaaaa tagcgcattt cctgatgcca ggcccggggc agcaacggca gtattttttc
    20101 cttgaactgt tcttcctcca ttgttttaat caattcttcc agtccagcca ccgaccgttt
    20161 gactggggaa acgatatccc gggtcaaaac ctcgggcaga tcatgaaaaa gtccggcaaa
    20221 aaaattattg tagcgtcgtt tggggttggc tccgatctct gccgagaaga aataaacgag
    20281 gatggctacg atgagcatgt gccccagcac tgaagtctcc ggcacccgcg gtgactggcc
    20341 ccatctttgc tgaaaacgca gctgccccac caggtccata aaattgcggc tttttttacc
    20401 cagcacaaat ttctgcacgc cggccaagtc atagaactct tcgacctcat tggcaatatt
    20461 ctggcgggtt tgttccaggc caaaaaatcc tgcattcatc cggtcgatga tggcaaactc
    20521 ccaattggtg gccagataat gagccgcacg caagatgcgc ttctccaatt tggcgtattc
    20581 cgtctcgaat aaataggttt gcagtttgga taaaaattct cctttgatac tggctacgct
    20641 gtcatggatc tgctcgatca cccagctgtt cagttcgcgg cttttttgct ccatcagccg
    20701 gtgaaaaaca tccggtttaa gatcagtcag aatactgcgc tgtaaaaact caaaaatacc
    20761 accttcgatc aagcgctgcc agtcaaccgc aatgccgcgg tcattttcct cggttttggc
    20821 cagcacatag gcaaagacca ttttgtggga ttgcttgtcc aattcactga acccggtatg
    20881 cggtctgata tgatcattcc agcgctgcat gctggccggg cggaacaaca gctccagcat
    20941 ttccttgcgg atcatcattc gttcctcctt aaaaaatatc tgcttgcggg aatgggcaga
    21001 gggactcatt ggttttcgcc atcaatcgtc ttgtttttca tattgcaggg ctacggcgcg
    21061 gaccgtttgg agaaccgaat cccgcagcgc aggcggttcc agcacttcaa cctgggtgcc
    21121 ccactgcaga atccagctct gcatctccat cagcccgcag acttcaaacg ccaaaataac
    21181 ggaaccgtct tcttcttcgg aaaatatcct ctgactggcg tggtagtgca gatttttcac
    21241 ccggtgactg acttcacgtg aaaacttcaa ttttacggtt tgcacctcgt cattgcgaaa
    21301 cacgccccag ctgcatccca gatacgattc aatggaaaaa tcgcccggat aggcaaacct
    21361 ttcgctttcc cgtacgttga gatgcaggat ctgatcgacc cggtaggacc tcatttcctt
    21421 gttctgccgg cgggcgatca aataccagac attccttttg cacagcaatc ccagcggttc
    21481 gattaaacgg gctgattctt ctccatcatt ggtcatgtag gtcagcttga cccgataaga
    21541 ctctcccacg gccagaatca gtttgcccag gatattgccg aactcctgcg ggtgggccag
    21601 ggtatcaccc atcacataga ttcgatcttt gagcatccgg gagcgttccg gaaaatgctt
    21661 gaaaaagctt ttttccaact gttctttggc ccccagcgtc gcttcatcca gtagcgtccc
    21721 gcgatagagc agcatgctga catacacggc ggccgcactc tccgaactga gcttggggag
    21781 atattcatcc tgcagacaat attttttact cttttctgaa cgttcaaccc agatccccag
    21841 attcgcaaag gtttccagat ctcgatagat catccttttc agcgcccctt ctgccgggac
    21901 atgatcaacg cttttggcga acagatccag ataaaccttc tcaatggctt tcatgctcag
    21961 gccaccgtca ggtatgctgt tgtgcagaca ccaatagaga tacagcagcc gctcagccaa
    22021 tttgggcgca cttggggcgc ggtcaggctt ctcctgcaga tacgttgcaa tctcatccag
    22081 caacagacgc tgcccattgt tacagatggc atacgcgccg ttgctgcaaa tctgccggtc
    22141 atcattcagt cccgccagga tactgtaaag cgaagctata ctgccaatgc cgaccgcttg
    22201 catcacttca tcccgatctg catcaggatg gttgattaca tattcccgca atcggctggt
    22261 gatctgtacc gtggaagctg tgcgattcat tttcaccacc cctgaccccc tttgcttgtt
    22321 cttatggtag atttccataa acaacaccta aatcctgttg gtgccggcga ataatcctac
    22381 gatgatatgt ttctttctct tctgaagata tgtgcttata gtgacactta tatgtcagcg
    22441 taatgaaacc cttgacgaaa tacttggcca ataccgtgtc aactattggc acgatattgg
    22501 cccgggcttg atcatggttt gcgtgactga gcaaaggaaa agcccgccaa agcgggctgt
    22561 ttagatgcaa aatttatgac tgtgatgaaa tggttcattc agctatcact gctattactg
    22621 cggacactgt tgcatcagtc catttattac cctttgctgg cttgataaat aaccatcccc
    22681 gccagcgcgc caaaaatcaa tgccaataca gccagtacag ccaccatggg agcacccagg
    22741 gacacataga cataaagcgc tacaataaac aaaccgagtt gggagatggc cgtattgcga
    22801 aaggccgcct tggcaaaagt caaatccttg ccggcaaagg aagccagata agcaaggccg
    22861 cagaaaaagg gtaccagcat caggaaagca aaagatacat gcggggaacc actgaggttg
    22921 ggcatgaagg cgagcagcaa caacagcact gccacggtcg cctgtcccag atacagaaat
    22981 ttgagtatct tctgtatctt ggctatcttg gccataaggg ccttcatggc ttcgggatcg
    23041 ggagccgggg cttggggagt gttcatctca gtcatcagtc tacctcctta aaatttgtta
    23101 tggcaaaacc atgacataat cttagcaatt tatacaaata atagcaagaa atagttatat
    23161 tcccccacaa tcacatgccc ctcacagttc catcccgagc ctcctcatcc ggcggtaaat
    23221 ggtgttgcgg gatacgccct tgacctgtgc aatccgactg atattgccgc catagatctc
    23281 cagcaattgc tcaagttcct gcctttcctg ctggttctgc agcaacttct gctgttggcg
    23341 gtatttgccg gcactctccc cggtcaggac cgtctgggcc attttttcgg gcaggcgtcc
    23401 ttgatcctgc ataatctcct ggggcagatg cttgatggtt atttctctgc cgttgctgat
    23461 atttatcatt ctttccacta cattttgcaa ttcgcgaacg ttgcccggcc aatcataacg
    23521 gcacaggcag tccagtaccg ccggttccac ctgcggcatc gacatttcca tcttcatgct
    23581 ggtctgttcc aaaaagtaat gaaactgcag cggaatatcc tccggccttt ctcttaggga
    23641 tggcaacttg attctgatga cattcagacg ataatagagg tcctggcgga aattgccttt
    23701 ctccacctcg acacgcaggt ctttattggt tgcacagata atgcgcacgt caaccggaac
    23761 cactttatca tcaccgattc gagtgatttc tttttcctga acggcccgca gcaaagagac
    23821 ttgcttccct aatggcattt cgccgatttc atccagaaac agcgtccctc cggaagccag
    23881 ttcaaacttc ccggtccgcc cgcctttccg ggcgccggtg aaggcgccat ccacatagcc
    23941 aaagagttca ctgcccagca attcgcgggg aatagcgccg caattcaggg caacaaacgg
    24001 tccgtgccgg cgtgaactgg cattgtgaat ggcctgggca aacaactcct tgccggtgcc
    24061 gctctctccc tgcaacaata tattgctgcg cttgtccgca gccaggcggg cggtttggac
    24121 tgcctccaac agtttgggat tctgtccgat gatatcctga aaggtaaaac tggcatgggc
    24181 tccgctgatc cgattgacca gactgcgtaa ttttttcagc ggattaaaaa tcagaacccc
    24241 cccgtttaca ctcccttctt catcccgaaa gggctggccg gagagaatgc agcttatcgt
    24301 tccggagaaa ccttgcaaat aagtttccag ctcattgatt tccgacccgc tgttaagaaa
    24361 ctcttccatt tttttaagct ccggaaaaac ggtcgtacag aattggtttt ccatctcccg
    24421 gctgctgcgc cccaagaggt tttccgctac cggattcagt tgcacaatgc gtcctgcctg
    24481 atcaatgatc ataaccccgt cggaaatgct catgaaaaca ttgcgcatct gttccgacat
    24541 ccttatgatt tcctggttct gggcatgcaa gcgcatctca aacattatgg ctgtagcagc
    24601 ggaaaccacc atccccaggg tgtgttgatg ggcttccggc gaggggccgg acatgttgag
    24661 cacgccgatg atttgctttt ggtcattaaa aatgggtgca gccgaacagg tccagacatg
    24721 atgcaactga cagtaatgct catcgctgct gatttgcagg ggaatctttt cctgcagcac
    24781 ggtaccgatg gcattggttc caacctcctg ctcagtccag gccgcccctt taataaaatt
    24841 gatttggcgg gcttgagcca acgtttccgg gttccccatc acctccatga tataaccgca
    24901 ctcactgctc aacatgacca aaaagccaga accggctaca tagcggttta attccttcat
    24961 aatcggcagg gcaattttga tcagctcggc atgttcccgg cacaatcgtt ccagttccgc
    25021 agtccccagc atggtgtgac tgcacccgtc ataaggatcg actccggcgg cataacaacg
    25081 ctgccaggag gctttgacaa tgggcgaaag gttgctgccg ggcagttcac cacgctcaac
    25141 aaacgccttc cacaactgtt ttgtatcttt caagggaaga tcattcatgg tatcccacct
    25201 ctttaacaaa aaactgccct ccctgcccat gcagcggaga gcagtttctg cctccagtgt
    25261 aacacaactg ttacatattt ttcatccctg tttatgcttg aaaagtttgc atattggcag
    25321 ccagtgtttt cctgcggcag gcttcgcaca gtttttcctg cccaaaatcg ccgtgctgtc
    25381 ccaaataatc gagttcatcc ctggttgcat aaggcttgcc gcagcgcgta caggcaacca
    25441 gcgcgaaagt tttgttccaa attctcctga ccgctccctc ttcttccact ttgatggcct
    25501 gcgtgggaca gacttgggca caggcggcac agcccatgca atcgtcggac gcttcatcat
    25561 agggcgtggc gatgcgcttg tcaacgccgc gaaacatact ccaaatcgca ctcttgccca
    25621 tttcggcaca ggcatggata caaagcccgc aaaggatgca gttggccggt tcgacaacaa
    25681 tggcaggcag gcgggaagct ccatatttct ggcacatggt ttccagcaaa gcatttttgt
    25741 ctgccctgcg agccagcaac atggcgatgg tacgccgaat cctcaggatt tgttctgatt
    25801 ccgttgtaat ctcggccggt tgggtaagcg gataggtgca ggctgccacc atgcgtgaac
    25861 gagtctgtcc ctcttccttg atttccacca tgcacatccg gcaggcacct tgtccgccaa
    25921 acgcctgatg atagcaaaag gtgggaatgg gaatgccttc tctgcggcaa agatctaaaa
    25981 tgctttctcc tgcatgcgct tctcgttgct gaccattcac gtttatcttc atcatcattc
    26041 ctcctagcgc agcttgatcg catcaaaagc acactgacga taacattctc cgcaatgaat
    26101 acaaatagat tcatcaatca catggacttg ttttttctca ccgcggatag cattgaccga
    26161 acaatgtctg acacacactc cacagccgtt gcatatttcg ggatcgatat ggaacactga
    26221 taaagcccgg catactccgg ccggacaccg ctgctgctgg atgtgttcca ggtactcatc
    26281 acgaaaatag cgcagcgttg acagtaccgg attcggagca gttctgccca aagcgcagag
    26341 cgaggcctgc tgcatggtca cgctgatatc ttccaacagg tcaagatctt caatgctccc
    26401 ctgcccttca cagatgcggg tcagaatctc cagcagcgcc cggattcctt cccggcaggg
    26461 agtgcatttg ccgcaggact cgcctgccag gaaactgaca taatagcggg ctacttccac
    26521 catacaggta tggtcatcca tgacgatcaa cccgcctgag cccatcatcg aaccggcttc
    26581 agtcagactg tcaaaatcaa tgggaagatc cagcagtgca gcaggaatgc atcccccgga
    26641 aggccctccg gtctgcaccg ccttgaaact tctttttttc aagacgccgc cgccgatttc
    26701 atagataatt tcccgcaaag tcgttcccat ggggacttcc accagacccg tattgaccac
    26761 cttgcccacc agggaaaaga ccttggtccc tttacttttg tccgtgccga tggctgcaaa
    26821 ggattccgca ccgtgtaaca taatcaccgg aacattggcc caggtttcca cgttgttgat
    26881 gaccgtgggt ttgccccaca agcctttttc ggtaggaaaa acatatttgt catgcggttc
    26941 cccgacccgg ccttcgatgg aagcaagcaa agcagtctcc tcgccgcata cgaaggcccc
    27001 tccgcctctg accagctcaa tgtcaaagga taactctgaa ttgaggatgt ttttgcccag
    27061 ataaccccgc tggcgggcat cgtcaatcgc ctggcgcagg tattgtacgg ccagatcata
    27121 ttcatcgcgg acgtaaatat acccgcgggc agcacctacc gcgtaagcgc ccagacacat
    27181 accctcaatt actgtatgcg gatcaccctc cataatgctg cgatccataa aggccccggg
    27241 atcaccctca tccccattgc aaatgatata tttgggattg ccggcagcat tccggcaggc
    27301 ctgccatttt tgtccggtgg gaaacccacc cccacctcgg cctcttaagc cggctgcctt
    27361 gatcgtatca atgacttcct gcctttccat ccccagcaaa gccttctgta aagcctgata
    27421 gccgccacgc tgaatataat cgtcgatttg tccaggatca atctctccga tattgcgcag
    27481 ggcaatcttg tgctgtttgc gataaaaatc ggtcaggtgg tgggaggtca gatgttcctt
    27541 gctgaccgga tcacggtaca gaagtcgttt aaccggcacc ccttgcagca gggttttgct
    27601 gatgatttca tccacgtccg ccggtgccac actgcaatag gttatgtctt tgggaatcag
    27661 cctgaccaga ggaccctttt cacaccaacc attacagcca gtcgccttta ctactggcag
    27721 cacttcgatt tccggatgat gctgcaggct ttgggaaaaa gccttgaata cttcccgccc
    27781 gccgttggca gtacagccgg taccacaaca caccagcacg gtctgccggt cacccttgcc
    27841 tggattcatc tcagtttgca tcgctctgcc ccccatactc cttgatgatc ctttccacct
    27901 cgctgcgatt gaccttgcca tagactttcc cgttgattac catcaccggt gccagcgcac
    27961 atgcccccaa acagttcacc gtttccactg aaaaaagtcc gtccgcagtg gtttgcccag
    28021 gcctgacctg caattgtttt tccagctgat cgagcagcgt ttcagagttt ttgatatggc
    28081 aagccgtacc gtcacaaacc cgaattacga aacgaccctt gggggttagg ctgaaagcct
    28141 tgtaaaaaga ggccatggca taaacacggc tgaccggcag tccgacatgg gatgcaatca
    28201 tggtaattgc cagacgaggc agataattgt attgtctttg gatatcctgc atgatggcca
    28261 gaagatactc gggcttcccg gcataggttt tgatcagact ctggtatgcg ctggcattct
    28321 catattcctt ggcatcgatt tgcatgcatt aacgcctcct aaacggaaag aattctgccg
    28381 gacctgggtt ttccaaggac ggcagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnagat tgaacagccg ttccagattc caaatccgct caccgatcag cagatattcg
    28561 tcatcactca ggtccaatcc cgtagccgtg cgcaactgag cagcgatttc cggcaaacca
    28621 atggcaaacg taacgaacag gcacaatccg gaagcatcca ccagggcagt tagatcctgg
    28681 aacagcttca gcaaagccgg tttgccttca gtgtcatccg ggtcaacctt gacagggatg
    28741 ccaagtattt ctggtgaagt catatatccg cgcacatggc aaccgccccg gttggaagtt
    28801 gcatagttca gaccgatgcc ttgctgggcc cgcggatcgt aggccggcat ttcctgtttc
    28861 ttcaccgtca tcgaatactc gggataacca tacttctctg ccaaacgata cgacccttcc
    28921 gccagttcat ccccaaatcc ttcccggtag gccgctttgg ttaccagatc aaccagcatg
    28981 gcgccatccc cccagcgcag atcggccgta gcacgaggaa taatgccttt ttccaccagt
    29041 tcaatggcac atgcaatcgt gcctcccagc gtaatcggat ccaaacccag ttcattgcaa
    29101 aggaaattgg ctctgcaaat ggcttcgaaa tcatccaccg ccaggttgga gccaaaggtc
    29161 caggttgctt cgtattccgg gccttcacca gcactcttga aaggtccttt gggaatgtca
    29221 gtaaccctgc cgcagccgat tgtacaacca aagcagcctt tgtttctgac cagatatttt
    29281 tctgtctggt attctccgga tattttttcc gcgttgggaa acactgccgc ttcactgaag
    29341 tttttgaccg gcaatccccc atgttcattc aggatgttga caagaatgtt ggtaccatag
    29401 gccgccagtc cttctccgct gacgggatgt tccctgagtt tggtgcgggc gtcaatagcc
    29461 gccgccataa aggccttggg atcctccacc acgattccca gggtgccttt gaccacgatc
    29521 gctttaagat ttttcgaacc catgaccgca ccaacccctg aacgtccggc agcacggtgg
    29581 tattcgttca taatacaggc atatttcacc aaattctccc cggccgggcc gatacagcat
    29641 accttggctt cctcgtccat agtcgccttg agcgcatcgg tcgtggcaaa aacatcctga
    29701 ccccaaagct ctgccgcgga tttcaattca acctggtcat tgtaaattga caaataaacc
    29761 gggctgtccg cccgtccctc aaagatgata ccatcccagc ccgcatattt cagttccggg
    29821 ccaaatgttc ccccggaatt ggatgccgca atcgtaccat tcagcggtcc tttggtcact
    29881 acattgtaac gcccgccaga cccggcaaac gttccggtga gggggccggt cataatgata
    29941 actttgtttt ctggacttaa tggatccacc ctgggatcta cttcttccgt aaaatatttg
    30001 gttcccaaac cccgggcacc aatatattgc tctgcatatt cccggtttag aggttccgct
    30061 tttatggttc cgtcgcttag atttacccgc aaaatcttac cttgccaagc aaacatgtta
    30121 tctcccctcc tccatgacgt ctctgaattt ttcggcattc gcgcggcgtt tattgaaatt
    30181 ggcggttttc gcctcacgat aggagatcgc gccggacggg cagtgtttca cacactccgg
    30241 atcgccgccg caaagatcac atttaataat taaatcatga tcagcatcat agaaagtcgc
    30301 gccaaaggga caggcaatgg tgcacatttt acagcgcagg catatggcat ggttgacaat
    30361 cttggcaccg gtcacatcat caacactgat ggcgcccacc gggcagacct ccatgcaggc
    30421 cgccacactg cattgctgac aaacggccgg aaatgaaaaa ccggtctttt cgaaagcaat
    30481 aattgagatg cgggaacgca agggattaaa ctccccggca tggatatatg aacaaatcag
    30541 ttcacaacta cgacagccgg tgcatttttc tggttccaca aatagcactt tgctcatacg
    30601 ttgctgattt caccctgcac gggcaaacca gtcctcactc tccttttgct tggatttgag
    30661 aaaatacctg tctgttgttc agccggacca tgacccgcca ggcaatacag agtccggatg
    30721 gcaaaaaaca atcgcatctt ctctgtgaat tattaagagc aaaatctgtg ccagtatttg
    30781 cataaccata aaaaaagacg ggaaccccgt cccgtcctgt gccaatccat ttataaaaat
    30841 acttgcttca tgctccactg ctgctccaat tttcacatcg ttgtagcaca catgttacat
    30901 gggtgtagca tttttttgaa accgttccac acccagatcc agcaaatcaa ccataccaat
    30961 ttcatgaata agatctgatt cgcgttttaa caacagttta atgacctcag ccgcctgcat
    31021 cccggcgatc accgctgcag taaacgacag cgtgccctgc tcctcttcga tgccggttcg
    31081 atctgtatta ctgtacaagg catggagtgt cttgtccccg ggcataatca ccgtaacctg
    31141 tccataccat cctcctaccg ctccgtgcac catcggcagc gcggcacgtc cacaggcttc
    31201 tgccagtgct aaccgggaag gaatgctgtc caacgcatca acaatgacct gctgctgtgc
    31261 aaatatgacg ggatcacccg cctcccaaag gcagggcagg gccgtcaaga caacctccgg
    31321 gtttatctcc tgtacatatt gccgacatac ctccgccttc ttctgcccca gcgtcttgta
    31381 actggccagc aattgacgat tgatgttgct ttcatggaaa acgtccggat cccatacggt
    31441 aatgcgcccg actcccagac gggccaaata attgacgaca tacaaaccca ggccgccgca
    31501 cccgcacacc cccacccggg catcccgaag ctgtgcctgc tcgcttgcac tgatggcttg
    31561 cagattgcgc aaataacgtt ccggaaccgg ttcaccctgc ccatgatgca tgctatccac
    31621 ctcccaccat ggggaatatg gacaaagtat cgcctgccag caattgttgg tcatcgctga
    31681 ccgataaacc gttcagaaat accacaccca actctttgcg cggcaggcgc agacaatcaa
    31741 ttacccagcc gactgtacat ccttcgtcca attccatttc tttttccttg aagcggcctt
    31801 tgcgaaaggt ggcaaaaagc ttgactctga cccgcatccc ggcaccactc cctcctaggc
    31861 tgagaaatct tcggcacagt tgatttgtcc aaagcacagc acacggttgt cttgatcctg
    31921 caaacggtat tcgtgatcgg tgtaataaag atagaccgta ctgtccataa agaccggacg
    31981 gccaaaggta atcgtaaaat gccgcagctt gatcccgccc atgcaaagcc ggctcatgat
    32041 caggttggct gaacacaggc cctgaatgaa cgtcctttca taaccaaaaa gcctggccat
    32101 caagggggaa gtatgtacaa tattgtagtc ccccgaggta tctccgtagc gtttggccaa
    32161 atcggcagga atatagatct cctccatccg tgcgctgtcc agtggttcag ccggcactcg
    32221 ggtaatgcct ttaaattgat cgctggtatc cttttgcaga taaaccgcat ctcttggggg
    32281 cagatccttg ataacaaaat gatcgcgcat tttcatatac tgttctccgt tgcgactgat
    32341 ggccgagcat ccgatcatcg ccgctttatc aggcttgatc tgcaaaacat cttcaaataa
    32401 atagtcaatg atataggctt ccccgggaat cagttcccga taaaaccaga tctcgctttt
    32461 aagatgaagc aaatgtttga aattggactg caacccttcg atcacttcca taaagccgac
    32521 cgctcgggaa aaatcggaat aggtaaaggg gactgtcaca tcctcgggca ctccgaaaag
    32581 tttataccat ttcttttggc cctggggata aagtacgaaa tgatcgtccc tgccaacccg
    32641 gcgtttttta aaaatgaccc ggtgcggacg taaaacacct ttgatcgcat ttttgtaaaa
    32701 aaacttctta taggccagcg ttcccgggtg tttacggtta tagactggtt cgacaactgg
    32761 ttccatgctg gtccctccgt tactgatatg atgtacaatc gttttgtttt ggaacgcgtc
    32821 ttcaaaaacc gtcccccttt ttatagcgga aacggtttgc tgaaagcaaa acagcagcaa
    32881 cagctcttgc agatgggtta ttcggctact gcgtaacagt tgcggccatt ttttttggcc
    32941 tggtacatgg caaaatcagc cgcctgtaca atcccttcaa catgcgcatg cgcaggggtg
    33001 tcgttacagg ccagtcccag gctgatggta accggtattt tatgattatt ataccgcacc
    33061 ggctgggcgt tgatcgcatg caaaatacga ccggcgagca gctgcgcctc ctcaagcgca
    33121 caaccaggca gcaccagcaa aaattcctcc ccgccgtaac ggccaacact atcataaaca
    33181 cgcagcgtat ctttgagccg ttgagcggtt tccacgagca ctgcatctcc gaccatatgt
    33241 ccatacgtat cattgatttg cttgaaatga tccaggtctc ccatgattac tgacaatctt
    33301 tcatgttgac gttcagcccg gataagttcc ttttccaaca ttttgatgat ctctccccgg
    33361 ttcaagatcc cggtcaatgc atcatgccgt gccatatgcc gttgtttctc cagcgcctcc
    33421 tgcagagatt gctgcaactc caatatgcgt ttgccggcct ttaaacgcat ttccaattcc
    33481 tgcggtatga atggtttggt gatgtaatca tcggccccgg cattcagacc cgcaataata
    33541 ttttctttgc tgttgagtcc ggtaagcaga ataatataat agtagttttc atcgtccagc
    33601 aagcgcagcc gttgacacaa ctcggtcccg tccatgcggg gcatgatcca gtcgagaatc
    33661 accagacggg gcgagtgctc ctgcaggact tgccaagcct gttcaccgtc ggcggcctcc
    33721 aggacctcat atcccatgga ggtgaccagg tcttttacca ataaacgaga gagtttgtca
    33781 tcatcggcaa ccaacacttt catctgcttc tttcccttca ctcgcaaata tgctccgtat
    33841 ggtatttccc tgaagggatc gccacagcgt tttatcctct caggaagatt gatgtttctg
    33901 tttggttatc aaccaataag attataaacc atctttcaaa tatttggctt ttttatttaa
    33961 attgatctcc cccacgcgcc gatctttcaa ccaggattac gaccggagag cctggaacag
    34021 atcactgaat cccggaaaaa caaaaaccgc tcccttcatt tataacggaa acggttgctc
    34081 atagacaagg taaaaaatat aaaaacaggt tgttttagag atatggttcg gcgaccacgt
    34141 aacagttgtg cccgtttgcc ttggcctggc gcatggccgc aatggcagcc tgtatgaggc
    34201 tctgcatatc cgcctgttcg ggatcgtcat ggctggccag gcccaggctg atggttaccg
    34261 aaatatcctt gttataatac atcactgatt ggtttttaac cacatttaaa atacgccggg
    34321 cgatcaggcg ggcttcattc acagaacaac cgggcatcac cagtaaaaac tcgtctccgc
    34381 cataacgacc aacgttgtca tatatccgca gagagttttt cattcttttg gcgacctcgg
    34441 ccagcacagc atctccggct gccttgccat gcatttcatt gatttgccgg aaattgtcta
    34501 catcgcccat gagcaccgac agtttgcttt tttggcgttg cgcccggtga atctcttttt
    34561 ccagaacatt gattatttcc ttgcgattca agatgccggt caaaacatcg tgctgcgcca
    34621 tgtaacgctg atcttccacc gcctccttca tcgtttgccg caattccagt atccgccggg
    34681 caattttcaa acggatggca aattcctgag gctggaatgg cttggtgata taatcgtcag
    34741 ctcctgcctg caatccggca acaatatctt cactgctgtt gcggccagtc aacacaatga
    34801 tatagtaatc ttctcctttg gaggatcgca ggcgtctgca taactcgggt ccttcgattt
    34861 tgggcatgac ccagtcaagc accagcagct ggatcgcctc ctcctgcaga atctgccagg
    34921 cacgctcccc gtcttcggct tccaaaacct catagcccag ggagtttaac aagtttttca
    34981 ccactaaacg ggaaatttta tcatcatcgg cgatcaatac tttcatcggt tctcctccgc
    35041 cttgccggat catggtaagg ttttccatct taattttata ctagtgtact ttattttctg
    35101 tcgctaataa acaccgtttt caataattta acaatgacct aaagattgcc gcggttctgc
    35161 cctatgctct tcgggcttcc aactgcgggc cttcgcgttc gatagcccgg cgggcaaaga
    35221 tttcgtcccg cagccaattg ttttgctcgt caggtcgtaa aatcaccggc atccgctcat
    35281 gaatgggctg cacggaagga ctggcggacg tcgtgagaat aacaaaaccg ggcagttcca
    35341 ggccctcctg ccggtaaagg gtatagatgc cggccatgta aagcagaggg gtgcccggca
    35401 aacgcagcag atacttttct ttttgcttct gcccggctct ttttttccac tcataaaacc
    35461 catgggaagg gatgatgcag cgccgctcca aaaaggcctg gcggaacatt tttttctcca
    35521 gcaccgtttc ccggcgggcg ttgatgatca cgccgctgcc ctgccaacgg ggaaagcccc
    35581 aactgaacaa acggggatgc acggcatctt cttcccaagc caataccgga accacatcgg
    35641 tgggaaagat ctctccgctc ttcattcggg catgttcagg ccagccttca taacgctgat
    35701 tgatttgccg tatgatctcc cgtgtctcga taatttcctc ttcggtagag accagatatc
    35761 gtccgcagat ggctaactcc cccttctttc cataaaccag cggtcttcct ccagaaatag
    35821 atgaatcgtt tggccgctta ttcgacaagt ataacggatg cctgtgccac cggctttgag
    35881 actggcagcc cggcgaatat cggtgacttt gtcgatggga tattcgcatc cgtcttccca
    35941 aatcaaagaa gttggcttga atccgccatc cggataaaat actgctttga cgctgacgta
    36001 gactttatcc gggttgcaat aacgtctttc ttccattatc gtcattagcc tcctttacgg
    36061 caatctgcct tccttaaaaa atgccaccgg atgaatgaca tgatcttcct tgggattgat
    36121 cgctcccagt ttttcatcct gcagcaacaa ggcccgctga atggagaaat gtccaaagcg
    36181 gtgccgcaag tcatcaaccg tggcttcgat tttctcttgt ttgcggcgtc tttcttcatc
    36241 ttcaaaaaga aacaactgtt cacaacagtc cgctgctacc aaatcagttc ccttgacccc
    36301 caaactgcgg acgggttttt cccaggcgta atgggcacgg aaaagcgtca tcgcctttgc
    36361 ggccagatct gcagcaagtt gggtaggccg gggaaacttg ccctgggcgc cgaggctgca
    36421 aagattgtta tcgcgaatat aaatctgggc ggtacgacat ttaaagccgt gctggcgcat
    36481 acgctcggcc acactctcac tgagcacata aaaggtcatc tctacatctg catccgtttt
    36541 taaatcacgg ggggtggtaa tgctgttgcc cacgcttttg atctgggatt cctcccccat
    36601 gcgaacgacc ggggagcagt ccagaccgtt ggcaaaggcc cacagcacct ccccccactt
    36661 gcccagcaaa gaacgcaggc cggcagggtc agccagggca atatcaccaa tactggtgac
    36721 gccccgggag gccagtttgc gtttggtggc ccggcctaca taaagcaagt caccggccgg
    36781 cagcggccag accgtttgtc tgaagttgtc gctggtgatg acggttgtgg catcgggctt
    36841 tttcatgtca ctgcccagct tggcaaaaat cttgttgtag ctgacaccga cggaagcagt
    36901 cacacccagt tcttctttga tgcgggttcg gatccggtca gcgatctccc gaccgtctcc
    36961 aaatacctgg caacttccgc ccacatccag ccaggcttca tccaagccga aaggttccac
    37021 ctgatcggta taactggcat aaatctcccg ggccaggcgg gcaaagcgta aatataagcc
    37081 aaaattgggg ggcagcacca ccaaccccgg acatttctgc tgcgcctgcc agatggcttc
    37141 accggtctga atcccggtgg ctttggcggg atagtttttc gccagcacga tcccgtgccg
    37201 caaaagcgga tccccgcaaa cggctaccgg tttatcgcgt atttccggac gatgcagaca
    37261 ttcgaccgaa gcataaaaat tattcagatc agaatgcaaa ataactcgtt ccttcatatc
    37321 gctctcacac cttaaggaac atttgttcta tcatgatagc actttcaggg cgtaagtcaa
    37381 gcaccgacct gaacgggccg acaaaagcaa gttccgtccg tgagcctggg cagggagggg
    37441 ataagatttt catcttttca atccgcaacc gcagccagta atcgtcctgc tgccttcggc
    37501 tcgctttcag ccccaaaaac ctcctggcca gtgcgttcag gtaagtcttc tccattgccg
    37561 ggaaagtgtt aaaatagaat tatcaagaaa acattttcgt ccgcacaacc agtcgcatgt
    37621 gctcagcaaa cagcatttca acaggaggag cagaatatga atcgatggca gcaggatgag
    37681 cttgattaca gcggaccagt gcagatcgcc gacaacatct attgggtcgg cttttccgat
    37741 gagaaagccg gtattcattg tcatccttat ttaatcatgg aaggttcgga agcagttttg
    37801 atcgatggcg gcagtcgcga tgatttcagc acggtgatgc tcaagatcct gcgcacaggc
    37861 gctgatcccc gtaacatcaa gcgcctgatc tatcagcatt acgatcctga tctgtgcggc
    37921 aatctgcctc actttgaagc agtcattaac gaccctgaac tgaaaatcat ctctcatcgg
    37981 gaaaacaatc tgttcatcaa atattattct tcggcctcgc ccatgcaatg tatcgaagag
    38041 ctgaataatt ctttcacctt ttccagcggc agggaactaa aattcattcg caccccgttt
    38101 gcgcatgcgc ctggcagttt tgtgacctat gacagccaaa ccaggaccct cttttccagc
    38161 gacatttttg gcagttacga caacaactgg cagctgtatc tgccatgcgc agatgtatgt
    38221 gccgtttgtc tgccccaggc aatctgcccg cataacggac agacctgccc cctgcatcaa
    38281 atgttatatt ttcatcagcg catcatgaca tccgccaaag ctttaggcta tgccctggag
    38341 caaatcgaaa aactggatat cgagttgatt gccccgcagc acggcagcct gatcaaatcc
    38401 aagcaagact gcaagatcat aatcgatcag ttgaaaagac tacagccggt gggcatcgac
    38461 ttctattttc aggagattgg ccaatgaata ctgatttaac acttgttcag caaaaactgc
    38521 tggatgccat caaaacgtat ccaaatgaag cagcgttgca gcttttgctg gaggattttg
    38581 ccgcccgcat cgaaaacaaa atccaaaacc ggctcatgac cgacctgatg ctgaaatatg
    38641 cggagctctc tcaaaaactg gaagcaacca acctgttcct ggcccaaagt gaaacccggc
    38701 tgcgcgaagc gcagcaaatc gccctgttgg gtcattggga actggatgtt gccacccggg
    38761 atctgcgctg gtcagatacg atgtaccaga tcctgcagat tgaccctgcg caaaagccgc
    38821 attacgaatt attcatgcaa cgggttcatc ccgaggattg tgacatggtt gccattcatt
    38881 atcggcaact gccggttagc gtcgaaccca atgaactgcg ttatcgatta ttaatgcctg
    38941 acaaccagtt gaaatgggta catataaaag caaccgccca ctttgatgcc gacggaaaaa
    39001 tactggccgc acgcgggacg attcaggata ttaccatcat gaaactgacg gaggaacaac
    39061 tgcagaaatt caatcatcat ttgcaggaat tggttgatga aaaagtgcag gaaatctccg
    39121 agtcacagat ggcgaccatc tttgcactgg tcaagttgtc cgaatcccgg gatgatgaaa
    39181 ccggcaatca tatcgaacga accgccagct tgtgccggtt aatggccgaa agaatggggt
    39241 tgctgtcccg ctatgcaggc agggtcaacg acgagtttat cgataatatt taccatgcca
    39301 gcccgcttca tgatatcggc aaggtgggta tccccgacgc cattttgctc aaaccgggcc
    39361 gattgacgcc agaggaattc gagatcatga aaacccacac caccatcgga ttcaacacgc
    39421 tggcccaggt ggaacagcgt tacacccaaa acaccttttt gcaaatgggt atggatattg
    39481 ccatcggtca tcatgaaaaa tgggacggaa gcggttatcc ccagggattg gccggtgaag
    39541 acatcccgct ggcagcacgc atcatggcca tcgtggatgt ttatgatgca ctgcgctcga
    39601 aacgggtgta caaggaggct tttagtcacg aaaaaagccg ggcgattatt gcggagggtg
    39661 ccggcagcca ttttgatccc tatttggtgg aattgttcat gcaaattcat caggaatttg
    39721 aaatcgttcg cgacagttta taatcccgga ccgcctgtga gccttctttt gaatacagaa
    39781 caagcccaga cgatatagag gattacaacc ggtaccatgg ccatggccgc aatcgtcatg
    39841 actttcagga tgtgggaact ggcggagatg ttatagaggg tcaggctgaa ggcttcattg
    39901 acagtggata ccataacccg ggggaacaaa gccgtaaaca aagcgacgat gatcgctatg
    39961 atggacacct cgttcaaaac gaaagccagt ctgctgaggc gtttgcacac acatatataa
    40021 gaaagcagca gcgccagcgc gccggccagg gtcagggcga tggcaacgga ggaagtaaac
    40081 atattcgtgg taatagcagc tacaacaccg acggcaacag ctgttgcaag ggacagcagg
    40141 ccagtgcggc gggcagcttg gcgagcctgt cgccgcattt tcccttgtag ttttatggca
    40201 aggaataccg cgccatgata gaggaaaaag accaccaggt ccaaaccgta aaacattgcc
    40261 ggcagggaca tgagatcaaa gagactgccg gcatattctt tttccgcatt gatcggcacg
    40321 cccaccaggc aaccggccag gaccactgcc agcaagaagg gaatcagcag gctggtcatc
    40381 cccaataacc agttccaggt tttgcgccag gcgggctgat gtaagcgatg ccaaaaagaa
    40441 atagccaccc cgcggatgag caaagacgcc aaaataaggg agaaaatccc tccataactg
    40501 ttgaacagga cggcatacca ccctggaaag accgcaaata tagctccgct cgcggctaac
    40561 agccaaactt cattggctcc ccacaagggt tcagtggaat gtatgatcat gttcttctct
    40621 tcctcattat gccccaacca ttgcatcagc atgccgacgc caaaagcgaa gccttcgaag
    40681 aagaaaaaac ctgcgaagaa aaatccgatt agactatacc agagttcatt caagcccatt
    40741 ccgccacctc ctctgatcca tctgttgctt cgtttttatt gctttgattc ccagatagat
    40801 gacctcggcg agaacgccac aaaagagcat caaatctaaa catcgcaggg atagttggca
    40861 attgattaca aatatatcgt accagatttt gggcaaattc ggatagaatt cacctaaaaa
    40921 tgatgcttgg gtgcgggtac ttatatcctc tgcaccgcgc ataaattccg gcaaaagcat
    40981 aaaagctaaa ccataagaag ccgaaaggag tatgcttgca tgaaaacact ctggaaaggt
    41041 gcggtcagtt tcggtttggt caatattcct gtaagcatgt atgtagccac cgaaaacaag
    41101 gatctaaaat ttcattattt gcatcaggcc tgcatggcgc ccatcaatta caaaaaaatc
    41161 tgctccaaat gcgagcagga agcaaaacca gaggaaattg tcaggggcta cgaatttcaa
    41221 aaaggcagtt acatcatcat gcaggaagaa gactttgccc gtattcctca ggaaaatacc
    41281 aaaaccattg atatcctgga ctttgtcaga ctggagcagg tcgatcccat ctattttgat
    41341 aaaagttact atctggagcc ctctgccggc ggagacaaag cctacacact gatcgtggaa
    41401 gccatgaaaa aaaccggcaa gatcgccatc gccaaaatca tgatccgttc caagcagtct
    41461 ctggcagcct tgcgggtcaa aagcgaacac ctggtggtag aaaccatctt ttatcccgat
    41521 gagatacgct ccccagcctc cctaagtacg ggggtcggaa ctgacaaatt gcacgagaaa
    41581 gagctgaaaa tggctgtgga tctgatcgaa aacctgtccg tcgattttga tcccgctcgc
    41641 tatgaagatg aataccggca ggctttgtgg gaattaatcg aagccaaaat cgccgggcag
    41701 gaatatgtca gcccgactcc tgagccgtcg ggcggtaacg tcgtggattt gatggaagcc
    41761 ctgaaagcca gtgtgcagat gaccgaaagt caaaagacca aaccggtcac gaagaaaaaa
    41821 gctgctgccg gcggcaaacg ggccaaaaca ggagcctgat tgtgacggac gagctgggca
    41881 acattcgggc gatggagccc atcagacaaa cagcggtgac ggatcgggat gattttgttt
    41941 accaggtcaa atgggatggc gtgcgcatca tggccggcat cacgaatggc cgggtttgtc
    42001 tgctgaacaa aaaaggcagg gaacgaacgc tgcaatatcc tgaattacag aaactgcccg
    42061 ggctgctcca ggccgatcgg gcgattctgg acggtgaaat tgtggtgatg aaaaatgacc
    42121 ggccccattt cccagcggtt atgcaacggg atcgagccca actgcccaac accatcaaac
    42181 tactggcgca aaccttgcca atcgagtata tggtctttga tcttttatct ttgaacaacc
    42241 aggatctgcg taaactcccc ttgttggagc gtgtggccaa gctggaaaaa gtgtttgccg
    42301 atcaggctca actgcatcat gtaccctgtt ttctccaggg ttcgtcgctt ttggcagcgg
    42361 tgcaggaggc cggcatggaa gggattgtgg ccaaacgaaa aaatagtgcc taccatcccg
    42421 cccggcaaca caacgactgg tttaaaatca aatgcagcca aagccagaag tgtatggtgg
    42481 ggggctatac gttacacggt cagaaagtga attcgttatt gctgggggtt taccgcgatg
    42541 atcaactctg ttacatcggc aaagccgcca atggtctgcg agaagagcat tggcaaaccc
    42601 tctcccggga gctgcccgct ctggagacaa aacgttcgcc ttttgccggg cccgttccca
    42661 aagaagctca ctttattgag ccccgcttgg cggtgctggt ggaattcctg gaatggaccg
    42721 aagccatgca gctgcgtttc ccggtgattc aggcatttat taccgccaac gctgcagatt
    42781 gccgcattta gaggaggttg acccaatgga taagacaaac acgttggaca tcgttggcca
    42841 tgaagtttcc ctgagcaatc tggatcggct cttgtggccg gaagcagggt atcgcaaaaa
    42901 agatctgatt gcttactacg gcgcagttta tccctacatg atcacccatc tgctcaggcg
    42961 ccccctggta ttcacccgct atccgggcgg catcgacggt ccgtcgtttt atcagaagaa
    43021 tgtcccggcc aatttgccgg aatggatcca aacctatccc tggacggaag ccaacggcca
    43081 aaccaaacaa tatgttttgg tcaacagcgc cgctgattta gtctggctgg ccaaccaggc
    43141 ctgcctggaa attcacccct ggatgtcgca gatcgatcgt atcgaaaacc ctgatttcat
    43201 cgtttttgac ctcgatcctt cggaaaacaa tgacttcacg gaggtagtga cggtagccag
    43261 gctgctcaag cagttgctga atgaactggg cctccgcact tatcccaaga catctggttc
    43321 attgggactg catatatatc ttcccgtagt aaaccgctat agctacgaac aagttcgcca
    43381 attcggccgt gccattgccg ccatcatttg caaggtcctg cctgatactg ctaccatcga
    43441 acgctccgtc cgccgccggg gccgcaagat ctatatcgat tacctgcaga acgggttggg
    43501 caaaaccgtc tgtgcgccat acagcgtccg tccccgtccg caggccactg tctcagcccc
    43561 ctttgcctgg gaggagctgg actgcctcgc tccgatcgag ttcaccctaa aaacaatgcc
    43621 gggactgctg gcccggcgtg gagatttgtt cagcgaagtt ctgactgacc ggcaggaact
    43681 cgaaccggcc ttgaaaatgc tgggtatttc gttttaataa aaaactttcc tgatggccga
    43741 tgcgtgcttg cctcagcgcc gtcccaacag atcatcaatg aattggcggg ctgagcggcc
    43801 cgagcggcca tgatggctca gctcccactg aattgcctgg ttgcgcagtt cttccgtttc
    43861 cattgggatt ccctcttttt cagccatctc cgttaccatc tgcagataaa gctcctgatc
    43921 gggtgacaag tagaccaggc tgatgccaaa gcggtcagca aaagaaagct tttcctgctg
    43981 ggaatccgga ggaaaaacct cttctcctgc agactggcgg tcgctccaat tctcccgcac
    44041 cagatgacgg cggttggaag taacgtaaaa gagaatattc cggggacgcg tttccagact
    44101 gccttccatg ctggctttga tgtatttgta atccacctcg aaatcttcaa acgaaaggtc
    44161 atcaataaag agaataaagc gataattgcg gtagcgcagc tgatccgtga tttcatgaat
    44221 atccgccagt tgacggcggg taatttccac catccgcagt ccctgttcag caaaaatgtg
    44281 cagcagcgct ttgacagatg aggatttgcc cgtgcctttg gctccgtaaa gcagaatatt
    44341 gttggccggc cggccttcca taaaggcacg ggtattgtcc aacaagacct ttttttgata
    44401 atcgtaaccg atgatcccat cccaggtaat cggatcaggg tgggcaatcg gctgcagacc
    44461 attgtcccag cgaaaagcac tgaagtgccc cagttgaccg cagccgaatt gacgataata
    44521 taattccagg gcttcc
//
LOCUS       DAOTHZ010000007        44201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_876_length_44201_cov_6.500362, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000007 DAOTHZ010000000
VERSION     DAOTHZ010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 44201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44201
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_44201_cov_6.500362"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="P5549_01850"
     CDS             <1..197
                     /locus_tag="P5549_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11848.1"
                     /translation="YRLYVKEELGAIIIKTTPPYIIFAINESNLTAAFWDYLTLLHNE
                     TRMDKKTVIKQLQKIVNALT"
     gene            complement(277..1452)
                     /locus_tag="P5549_01855"
     CDS             complement(277..1452)
                     /locus_tag="P5549_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW11849.1"
                     /translation="MDLKMLLKISVNSILANKLRSFLTMLGVIIGVCAVIILVSIGQG
                     TTASITESIESMGSNLITVRIMGRGATTTLTYEEAKAFAELDGIIASAPVASGSVTIK
                     YGNNEMENISLMGVDSDYKIVRNRTIQGGRDILSLDVENRQKSAVLGVDIINELFSAG
                     TNPVGKTVQINGTHFTVVGVLDESGSSMGASNDEIILIPITTAQRFLNSSGISMVYVS
                     AENAEVSTQAVAAIENELYEKFKQDEDAYRVSSQDDMLETVSSISQTMSLMLGGIAGI
                     SLLVGGIGIMNIMLVSVTERTREIGIRKAIGARRRDILTQFIIEAAVVSGMGGIIGVL
                     LGWGGCSLISSAGINTAISLPIVFIAFIFSLVIGLIFGILPARKAASLNPVDALRFE"
     gene            complement(1453..2127)
                     /locus_tag="P5549_01860"
     CDS             complement(1453..2127)
                     /locus_tag="P5549_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW11850.1"
                     /translation="MIRLENIVKTYQMGEEIVHALQGVSLHVPAGDMIAVIGPSGSGK
                     STLMNMIGCLDVPDEGQYLLDGQAVDCLDEDELAQVRNRKIGFIFQQFNLLPKLTAFE
                     NVELPLIYRGMPPRERREKAEAALHRVGLGDRLKHKPTQLSGGQQQRVAVARALAGDP
                     PIILADEPTGALDSKSGEVFMQALRELNESGITIVLITHDMHIAQQARHFVSIRDGLI
                     VDRGVN"
     gene            complement(2130..3761)
                     /locus_tag="P5549_01865"
     CDS             complement(2130..3761)
                     /locus_tag="P5549_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRW11851.1"
                     /translation="MKKIWAAIVAKAGRKRLIIAAVAIVLLAALWYSAAENKNSTADA
                     LQTMKSVAVVKGNIEETISGSGTIEAIDRKEIASECTGTISKLYVSEGQQVQKGDLLM
                     TFDNDNFDSQVQSARLNLQQAQQEMASLQQDKKNLKIVATASGVLGDSLPAVGDNVGK
                     GSSFSTITDQTHMQARASFNLGTELNISTGQEADLFVADYLTGIKGTVTSVSTINAAG
                     GNRICDVLITVSNPGRLTSGTTVTKVEVPTTGRTIAAIQGTTLEWPSAANVQAKSAGT
                     VAKLYVTANQWVEQGQILAELESSDLDEQISNQRIAIQQKQLSLAQQNRELEKRTLYA
                     PIAGTVVAVNVTEGEEVTDNQSNLVTISCLNTLQVTIPVDELDILNVKTGQSARVSSD
                     AIKGQVFTAKVTEIAGEGTVSSGVSTFDVLLLLDDPGALKAGMNVNAEILLAAKEDVL
                     LLPLDAITEQGDKKFVKIRSSNGEEKRTEVKVGIVTSTTAEIKAGLQEGDNVVYNALQ
                     ATAKTSSSITQQRQQGGMMGGSGGRTGGPPPAGGF"
     gene            4080..5156
                     /locus_tag="P5549_01870"
     CDS             4080..5156
                     /locus_tag="P5549_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW11852.1"
                     /translation="MLHTKIMVVDDSNVDIALIKSILTDYDLITAGDGVEAMELLQEY
                     PDTEIMILDLNMPRMNGFEVLEAMRKHPVYKKVTVLILTNYDETENEIKGLDMGALDY
                     IRKPINIQSLRKRIQVHTHLRRARQQLQRYNEILEKTVQERTRELVITRDVSIHAMVI
                     LLEKRSIESSHHAKRTQWIMKALCEHLSSQPIYQNVLTEEYIKELFDTAPLHDIGKVG
                     IPDRILLKPGPLDERELAIMKKHTTYGVEALQLGNDENLSVSFIKTAIEIAGTHHEKY
                     DGTGYPNQLKGEEIPLSGRLMAVIDVYDAIISQRVYKPPYEHTTALAYLLEERGKSFD
                     PTIVDAFLQIEKMVEYISKTFKPY"
     gene            5177..8101
                     /locus_tag="P5549_01875"
     CDS             5177..8101
                     /locus_tag="P5549_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRW11853.1"
                     /translation="MKMIRILMIAILITFLFPMISLASSPSLDLTPEEKTFIAEHPEI
                     RLGVDPKFIPYEFIDTDGVYKGIAADYIKLISQRTGLTMTVAPDLTWPEAYEKAVIRE
                     LDALPCIAQTREREKHFLFTQPYYTFQRVIYIKEDNTSINSLDDLKDETIAVQKDSSH
                     HSFLLSYPGLKLHLYTNVEDALRAVASGEETAFIGNLATSNYLIKSYGFTDLKYIKMA
                     GEDEKSLYFAVRTDWPLLVSILDKALASITEEEKLAITDKWIGINENPDYGRLIRMAG
                     MAGAVIALALMVSFFWIRRLRKEIKIRKQIQDELNIAKNEAESANQVKSMFLARMSHE
                     IRTPLNAIIGMAYLLRKTDINATQRNYLHKITEASNNMLAIINDILDFSKIEAGKVEI
                     ENVPFNLDNLLSEIINIISFKVEEQGIHFTLEKDPDMPEIFKGDPTRIGQVLLNIVNN
                     AVKFTPQGMVSLSVHLAKKEMTTCRLQFVVTDTGIGMSEEQLSQIFKPFDQGESSINR
                     RFGGTGLGLSIAKNLLDLMGGEITVESTPEQGSRFTIQLELEAEPSCTREENRKKNSV
                     FFKNLHALVMEKSAMGTELLKKYLHSFAIAADFAASDAEAMRLIEQSGNGSKPPYNLF
                     IIDFETPKEGGIGFFNFWKQAWSASEEMPKCIMMVPLTRADLHEAAEAAKIDFTINKP
                     VLPSVLYNAIVEIFNPAMLEAQGKSLLTSDLAKPVTDYPYHVLIVEDNKTNQFIAKTI
                     LEQSGLRTSIAENGQEGYDCFLALQDELDAILMDLHMPVLDGFEATALIRTKNKKIPI
                     IAMTADAITGIEEQCRRIGIDHYVSKPFDPDQFVATIIEVIQSQPRMVAATEKSKKQT
                     AAARDEEAALDVAAGLNLLGGKEDLYDMVLQQYLLENESVPQLLQAAIAKLDYKNAVQ
                     IIHKIKGSSGNIGAKTLHKTARELQQALSEENTAEIDRLHHRFLQELQQLLQQIQQRL
                     QK"
     gene            complement(8166..>8420)
                     /locus_tag="P5549_01880"
     CDS             complement(8166..>8420)
                     /locus_tag="P5549_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OST-HTH/LOTUS domain-containing protein"
                     /protein_id="HRW11854.1"
                     /translation="KESMASLDELIEAIKGIVTESSDEDGWAFLGEVGKKLNKRYSDF
                     DTRNYGYSKLTPLISSLKQFEIQSRKSSRPHITLKYIRNK"
     assembly_gap    8421..8520
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8582..9418
                     /locus_tag="P5549_01885"
     CDS             8582..9418
                     /locus_tag="P5549_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW11855.1"
                     /translation="MISDNPVLGEGVQVNYSIEQSPDVLNAKLLSGEIELATIPTNTA
                     ATLYNKGVPYQLASMNTWGVMYVVSNDQAITSWADLKGQTIGAVAKGASSDVVFKYLL
                     SKNGLDPDKDVTLNYNPSPVELAQLVIAGKVAVAALPEPWVSVVLNKNPQIKVGLDLQ
                     QEWRKIAGEDVPFAQTCLVVNRDFAAQHPQIVETFLAEYQQAIDWVNAHPQPAAELIK
                     NTDIGIPADVAADAIPRCNLKYMGSQAAKPAVTQYLNVLLQFSPETVGGKMPDDKFFY
                     QQ"
     gene            9393..10148
                     /locus_tag="P5549_01890"
     CDS             9393..10148
                     /locus_tag="P5549_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRW11856.1"
                     /translation="MINSSTNNKIITALSILAMLLTWHGLALLIGKEYILPTPAQTGL
                     SLLLIMQSQDFWPAVLMTVGRGLLGFLISMGLGIILGMAAGFSRVINWLLRPWVTVMR
                     STPVMSVIILAIIWFESNLVPVVVAILMLFPIIYGNVVAGIRNINQEWLEMARMYKVK
                     PRRIIAELYFPAILPYLLAGATTAMGIAWKVIIAAEILSQPTRAIGTDLMIAKFNFAT
                     AQVLAWTVVVIMISFVFESLLGAWENNLTNWRR"
     gene            10150..10863
                     /locus_tag="P5549_01895"
     CDS             10150..10863
                     /locus_tag="P5549_01895"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW11857.1"
                     /translation="MAFVLDKINKGFGGLEVIKDLSMQEEENRIVCILGPSGAGKTTL
                     LNLMSGVMKADSGQISGFSGHSISYLFQETRLLPWKTVRENIDFVLKDKLPRHQREQT
                     IERYLEMVELSPFASYYPARLSGGMKQRVSIARAFAYPADVLLMDEPFKGLDTQLKMS
                     LIKSFIRLWTLDRRSVFFVTHDIQEAVLIGHLVYVLTDRPASIKGRIINPVPHEQRDD
                     SNAPELQSIEQAIYQLVTL"
     gene            complement(10945..11613)
                     /locus_tag="P5549_01900"
     CDS             complement(10945..11613)
                     /locus_tag="P5549_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW11858.1"
                     /translation="MGITRDFGDKVKTRVLFGVDLEIRRGEFTSLIGASGSGKSTLLN
                     IIGALDRPTEGKVIIDGTDLGTLDDDGLALFRNQTMGFIFQSHFLLPQFTVLENVMIP
                     YLIYAGRATPTVKKRALDLLERVGLSHRRDYRANAISGGEQQRAAIARALINQPRMIL
                     ADEPTGNLDTSNTENVFAMLREINREFKTSFIMVTHDRALAERTDRLLIMRDGQIVED
                     ICHI"
     gene            complement(11630..12850)
                     /locus_tag="P5549_01905"
     CDS             complement(11630..12850)
                     /locus_tag="P5549_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW11859.1"
                     /translation="MRKNSIRFEFSLASRFLTENRMQTFLIILGIAIGVSVMVFLSAL
                     IDGLQANLIEKTVGRSPHIVISSDENAAAEAVKSLNGNPVLVLDATRKTMRPMVEWRN
                     ITAGLTADQRIKTALPVVEGSGLIERGQISRAILLKGFDLNQADRIYNLTGSLVAGSP
                     NLPDGAVLIGKDLAADLGVSVGEPITLELSGRDSLPVVVDGIFDLGVSAINQRWMVMD
                     QRRAAALLGMGDRVNTIEVQVHDVLQADALAAEWSQRLPGLAVESWQESNASLLAGLR
                     SQSSSSYTIQFFVMLAVILGVASVLAISAVQKSKQIGILKAMGIRTGSVSRVFMIQGL
                     VLGTLGTLSGLGLGLLMSKAFIVLAKQDYTLLLKPMTTAIIVLSTIVAAVLAAYLPAR
                     QVSQIDPIEVIRNN"
     gene            complement(12854..14050)
                     /locus_tag="P5549_01910"
     CDS             complement(12854..14050)
                     /locus_tag="P5549_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11860.1"
                     /translation="MLQHKKTIVVTAILLAAALLLFVWLRPTQVNAYKVTQQDYVPSL
                     LLSGEVIADGSTLLSATAAGKVLACPIGKGELVRHGQLLVQIDDAQAHLDRDRAAAAV
                     QMARAQLQKASAVTREEARARSIQADLETEKAAAQYERVKSLHASGAVSETELEEAAH
                     QLQVKQEAARSARAQLESLASQGSAMAILQAELQQRQLDLQEKEILLNEYKIVAPADG
                     QLLDLYVKPGEMLSKGSRAVLLAAGQGLRIKVQPDQRYAALAALGNQAKVWITNAESK
                     KWDAQVVYTDPLGNAEQGSFTAELDFVGETPDLYPGQLLSVQLFAATQSDAVILPDRY
                     LSEQAGQSGVWLAVNSQAHFAPVQIGLRTGDGVIITQGLKADDLVLEPSGWREGATVS
                     VRSGKG"
     gene            14617..16446
                     /locus_tag="P5549_01915"
     CDS             14617..16446
                     /locus_tag="P5549_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRW11861.1"
                     /translation="MTAKYSYNPRDAKFLLKEWLPLEDILQYERYQELSLDDIDGLLE
                     QMNRMVAKVIAPTNDDGEINHPRLVNGRVVGPPTWKDVYVYLQQQGWGTGNFDRHAAG
                     TLPEVVYAALAEMMCAASPAFIYYPALTTGAFKVIQSYASPALQEIFLPGMMDGRFQG
                     CMCITEPSAGTDVGDGLTRALPTDEPGIYKIKGSKIFISAGDGDHAENFIYLTLARIA
                     GAKPGTKGLSLFIIPRFWINDDGSTTPNDVETTGVENKFGIRGCPTVSLAYGDNDQCR
                     GYLVGHPPDEQGNGEGMAQMFQMMNGKRLECGLTSAGVTANEYWNAAAYCRERIQGRP
                     LNNPRADRVPIMQHEDVKRMLMLNKATTEVIRSLVMKAYFYQDISDYDPDPDRRKWAF
                     ARLECLFPICKAYTSDEAWTLICESIQIYGGYGFTEDYPAARAVRDTKINSLYEGTNY
                     IQSMDLITRKWPLHKGQAFAGLLQDIESLITSERAAYPELEREFAKLEKALRAYQELQ
                     GITLGYFQQGKIELVASFARRILTATGQLLGAHFLLEQAVIAQSHLRELEKDHYDYNY
                     YLGKTLSARFYLNQILPNVWNTTDLLREGDPTVLHCPDEIFAF"
     assembly_gap    16730..16829
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16830..18478)
                     /locus_tag="P5549_01920"
     CDS             complement(<16830..18478)
                     /locus_tag="P5549_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW11862.1"
                     /translation="MEKPWLKEFEADVAKWRPLWEKHYDEGVPKNFEYPDYPLKYWFN
                     KWAEETPEKPYILMGDVAINYGTANNLARRIANALLGLGVQKGDRVAIMAPNVPQYIL
                     SLQALFKIGAIEVPTNPLYTIPELALQFKDSGTETVIVMAAFADKAIQIMQDAASPVK
                     RVIVFQVPSAAVEIPPGEGIYDFNQLAMAASDAEPDIEVFADDVARLQYTGGTTGIPK
                     GCVLTNAIIFSQANRTATWTSGAFKLVSKEEVRTLCAIPLNHIYGFNANVNVCLFTGG
                     SIVLVALPTPDNLLEAIERHRPNIWAAVPAMIIGLNNHPKIASADITSLKGIFCGSAP
                     LAVEVMNEFERLTGGRILEGYGMSETVNILTVTPVFTLRKPGSCGIVWPDTDLVVVDV
                     DTGTQVIPRNELGELIGRGPQIMREYWNNQEETDNVLRDGWLYTGDIVRIDEDGFVFI
                     VDRKKDMILCSGFNVYPRDIDEVLYAHPKIMEACTVGVADPKRGETAKAFVVVKPGEV
                     LTAEEVIEHCRKSLAPYKVPTLVEFISEIPRTSVGKPDRKVL"
     gene            complement(18851..19384)
                     /locus_tag="P5549_01925"
     CDS             complement(18851..19384)
                     /locus_tag="P5549_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRW11863.1"
                     /translation="MEDRDRIIITGSGNVAFYVYQFASFLDYEIVIFDNQEERLTPDR
                     FPLASERIGGDIVENISSYEIRSTDSIVIVTHRHHFDEAVLKTVIASPARYIGVLSNR
                     KQVVAYFGKLTALGIQDERLERIHAPIGLDLGGQKAVEIALSVVAEIQAVRYGRAGGF
                     MLLKSRHSSKEKRDNLF"
     gene            complement(19592..20122)
                     /gene="hpt"
                     /locus_tag="P5549_01930"
     CDS             complement(19592..20122)
                     /gene="hpt"
                     /locus_tag="P5549_01930"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRW11864.1"
                     /translation="MPAPGLGRMIYTQEQIQTRLREVAEEINRDYAGQKLVIISVLKG
                     SIYFLADLTRQLHIPLSIDFLAIAASPPSENHSGGVHFTKDLDINLKDRHVLLVEDVI
                     GTGLTLGYVIQHIENAGPASFKICTLLDNPAGRIVTVKIDYRCFTMPDLFLVGYGLDY
                     REEYRNLPYIAEFHRP"
     gene            complement(20122..21051)
                     /locus_tag="P5549_01935"
     CDS             complement(20122..21051)
                     /locus_tag="P5549_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRW11865.1"
                     /translation="MLITVSRETGSLGREITDLLAMKLDLPVITRDLVMNQWFPEIAD
                     KHELHMLSESPRYFLNTSAEGITYAAYLENRLKAYAAQHSAIVFGLGAQMIFAGQPGV
                     LHVKIMASPQIRNRRLMQSLHIGEKEAQRFLELTDRKHRRYIATLYERDWADPTLYHL
                     MLNTDCISEEEGAEVLAFMARHLQLGTALPAPAADEDSVRQVVFKHPSEAEFATILDM
                     HHIQWEYEPRTFPVQWDTEGNVTQAFAPDFYLPRFDTYIELTTMDQKYVSQKKKKVRM
                     LQKLYPDININIVFKNDFYHLAERFGLRKGEDD"
     gene            complement(21757..22245)
                     /locus_tag="P5549_01940"
     CDS             complement(21757..22245)
                     /locus_tag="P5549_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRW11866.1"
                     /translation="MIRYKKLFFWLPVLIWTGSLFFFSSQTADESTRLSGEMIRTLLV
                     FIRPEFLQMTAAQQAQWVASLQHLVRNAAHFGSYFVLAMLCMIALRQYPWRLARQAAL
                     TLLLCMGYALTDELHQLFVAGRAFEMVDLGLDLGGTLLGLAVMLVIAGEKQSHIKRQA
                     PR"
     gene            complement(22245..24848)
                     /locus_tag="P5549_01945"
     CDS             complement(22245..24848)
                     /locus_tag="P5549_01945"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HRW11867.1"
                     /translation="MIQLPYNRDDQSVLQELGVDPANGLSSEEAARRLAEQGPNKLAE
                     GQKKTTLQLFLEQFKDVMIIILLIAALISFVVAMYEGEGYFEPFLILLIVVLNAVIGV
                     VQEGKAEKALEALKDLSAPQARVTRDGREMMIDAATLVPGDMIRLEAGDFVPADARLI
                     ESNSLKSEESALTGESVPVEKDAGVIVNADAPLGDRLNMVYSGCSITYGTGRAVIAAT
                     GMNTEMGKIANLLSTAEDTQTPLQHKLAEMGKYIGFFALAICAVIFVIGLTSGMPMME
                     IFMVAVALAVSAIPEGLPAIVTVVLAIGVQRMVKKNAIIRKLPAVETLGSASVICSDK
                     TGTLTLNQMTLVKAFSAAEILTESIDADNSAPIKKLLQYATLCSDGSVEIDENGRETH
                     IGDPTETAIVLAAYKNGLPKDELNRLYPRQCELPFDSDRKLMTTVNQIDGKYVVIVKG
                     AFDVLAERCLAGNVEAGRQMTDELSSQALRVLAVAWKAIDALPETITPVVLENGLTFM
                     GLLGMIDPPRPEARDAVAVCRAAGIKPVMITGDHVVTAAAIARDLGIMQEGYEAVTGA
                     ALDAMPEEELQRRVDHISVYARVSPENKIRIVRAWQQQDAVVSMTGDGVNDAPALKAA
                     DIGCAMGITGTDVAKGASDMILTDDNFATIVDAIREGRGIYDNIKHTVGFLLGTNIGE
                     VLVVFIAMLVWQVSPFLAVQLLWINLVTDSLPAIALGMEPVEEDVMRRRPKPKGESIF
                     AGGFGLRIVLQGTMFALLTLIGFMSVWKSTGNIEAARTMAFIVLAGSQIFHAFNMRSP
                     HSLFTTGFFTNRYLNLAALASLILICLVLFIPPVADAFGMVQLPSSLYLVGLGLALVP
                     IPVLELYKRIV"
     assembly_gap    24994..25093
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25207..26100
                     /locus_tag="P5549_01950"
     CDS             25207..26100
                     /locus_tag="P5549_01950"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRW11868.1"
                     /translation="MDENLLIYVFDPQDQLVYLRDVFKKKLPLSHALLARLKTQEKIH
                     VNGMFAHTNYRLQPGDVVTVDLNLAETNPILPQDIPLEIVYQDADVMVINKPAGMAVH
                     PVKAKAEGTLANAVTHYWQQQGRFCLFRPVNRLDKGTSGLVLVAQSQYAHQALFRQQK
                     LGQIQRRYLAVVEGVVEKDQERIELPIGRSQPGSSVFRQVDAAGKPAVTHYTVIQRYP
                     RHTLLSLRLETGRTHQIRVHLAHLGHPVCGDSVYGQASPLIARQALHAAWTSFLQPRT
                     QTRLEFAVPLPPDIQHLLTLI"
     gene            complement(26166..28349)
                     /locus_tag="P5549_01955"
     CDS             complement(26166..28349)
                     /locus_tag="P5549_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW11869.1"
                     /translation="MKKRLRKGMSLLMMVLLLLSLLPAGVMAAEKEAALSLEQAIQKV
                     KQNFTIPAEYTEFTSSFRSRDNIQIWSLSWTDKENRLGSFNAEVDATAGLITSMHIYK
                     QGRQTTEIPAVSIDQARQIGLELLKKLIPDRVAELQYVPNQQIISLSSWQGGQYELRW
                     QRMANQIPVGNEGVTLQISTGDGTVNSYDLNWSDKIIPAKTEFISAEKAAEVFAANKM
                     LELQYYIAPKYSILREGDEKAQPLLVYRLMHNSGGMIDAASGQPIENQSIYGINDVGG
                     MGSMEKSPAPQADLSPQEQAEVERTLNLISQEKAVSMVKQWVSLPANVKLEEANLYKD
                     YQDPEKRTWNLNWRNDTEVNSSAYVSAQVDAVSGEIRNFYIDRGDLNDVKPMLDKAAA
                     ERIARDFLARIQPSKQNSVRLEKDVYQEIYVKESPYPAQWQFQFTRLENSIPCLNQGI
                     SIAVDGRSKQVSSYRLEWPTLTFSDPAQVMNMEKANQVYLQHQPLTMRYELTYPNLLT
                     RNESSGEYKLVYIPRAEVQNQSSNMFDAVSGEPLSWDGTPVAEALQPQAFTDIAGHFA
                     EKEISMLGQAGIMTEYGTTFHPHETINLVTMLRVMVMARNGYYEPVRGSDEEIIDQAL
                     RSQWLKEKMDPQTPMTTGLLAQLMVRMLDVDFIANMPSPALQAPFRDFNSMDANLQGY
                     AALCWGLGIIKGDGVNFNAAHHTTRGEAAAVLVRTLTTANQKSNF"
     gene            complement(28893..29360)
                     /locus_tag="P5549_01960"
     CDS             complement(28893..29360)
                     /locus_tag="P5549_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11870.1"
                     /translation="MAVSLLVLLGVVALVVLFIVGVRGESKNGGEDMIKKVYVYLVLF
                     ATLMMTIGGGVAAFMAAADIVAPTAYHQTFEDYKLYSTERMVKEGGVTTEVSKLTEEQ
                     LKANYDAMVVAEKQRQIDRAKNSLIKSLGWIIIPFPVFLYFQRKLKAGGFEGV"
     gene            complement(29629..31350)
                     /locus_tag="P5549_01965"
     CDS             complement(29629..31350)
                     /locus_tag="P5549_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRW11871.1"
                     /translation="MSIIPLQENLMEKWKLPLLTDYIKKWAAITPDNPALVSADTGKS
                     YTYQEFDEMITLYALRLKKMGIKKGDIVVAQWLAWPEFYMLTYACATVGAVMTPLDVR
                     LMGHEVVRDMSKINPVAFFCLGNTPIRDFRDLVKEVQQAVPSVKHYIQYTPGGKEGDF
                     LEGCLNFDEYFAPAALEELKQDSALIESLRQEYDQLGKRDAHIIIFTTGSTGEPKPAL
                     ICHENTITNNAVFSREVGLYGTDSRYLNSMPTSHVAGTCQGPMTVWFVGGTVVSLSMY
                     NPQAVLGCIQKYRPTWWGGVPTMFRMLWLDPNYASYDLSSLRYILYGGSAVDVPFLEK
                     MSTMAATFGTSLGMTECAGYFTATPRGISLEEMAGQVGQVFPEYAPVTIRQPMNEDGT
                     AGEEMPPGEVGEICVHGPIVFLGYYNNPAATAAAISKEGILYTGDMGYFHDFGSYMGL
                     KFAGRRKFVIKPKGFLVFPDEVTDYISRHPKVAQAQVLGMPHEVFVDGVFAYVQPKPG
                     VELTPGEILEYCKNMAAYKRPIHVELWPVDEPLPATRVGKVNVPALIELAEKVVARLR
                     SEGKWDK"
     gene            complement(31555..33465)
                     /locus_tag="P5549_01970"
     CDS             complement(31555..33465)
                     /locus_tag="P5549_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW11872.1"
                     /translation="MAGKSNRATDREKRSWEEEIKNYINDADSYRQLTENIPVLVYVY
                     RNSRVIYVNPAVEKTLGFTFEEMTQKDFWDICHPDYRDLIRERGLARLQGKEVTSNYE
                     FKIVKKNGEAIWVDVFFATTRMKGEILGIVGAYDITEKKLLKEQLQAARDELELRVRQ
                     RTEELSLKNNELTLLNRNLNNVVTNMSDGVVVVNRKGEIELLNQVFKQNWGDWLEKIR
                     TKLQKDVRTGKSHFINDLFDKRISFQDEEVIYGTPGDQVHFLASGTPIVNEAGVVVSG
                     LLILRPIKEIRQLVNRFSGAKATFRFEDIITGNGAMLDLIENAKMAAASTSNVLIEGE
                     SGTGKELFAQSIHNYSRRSKGPFIALNCGAIPRELIGSELFGYAEGAFTGARKGGNPG
                     KFELASGGTIFLDEIGDMPFEQQAALLRVIQDKSIARIGGNQAIPVDVRIICATNKDL
                     HTEMMHGRFRQDLYYRLNVISIRIPPLRERREDIRLLFHHFLRSNDAFRENDRDCLAP
                     KIMGHLEAYDWPGNVRELQNVAESMLLAARGGRLSDMELPSGTLAAKLLHRETPVQKS
                     MPAQEDGEGKSIWDARQAGRQQQEDRERARILELLQLYNGNISRIAREMGMSRTTLYK
                     KMSKYKVDDQTV"
     gene            complement(33650..34609)
                     /locus_tag="P5549_01975"
     CDS             complement(33650..34609)
                     /locus_tag="P5549_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRW11873.1"
                     /translation="MQERNRIRLIVDIVLTILFIALLYAENTGFTFHEIAGLAMGALL
                     IYHIVLNWSWIKSIGKNLFNPRLKAKPKLFYLLNTVSLLAVALIIVTGVQISMVLFPA
                     DEMISHTLIDVHKWVSYACVGLFGLHIALHWQFIAKNIPRLFRASDRSAWGRAALSTA
                     AVMLILSLLYTQIAVVSKDVSSDNRASESNDMIARQSVQKQDEIYTDRPRRGHGHGRI
                     AQQEYAYYDQKSSPGSTSNADKIAVPGSGDSLHQQDQNNETSTDSNIASDNGNDKPSL
                     NEYLGSLFCDACEKHCSLLSLRCDRGIPQLEAAQQQYQSIYVQ"
     gene            complement(34763..35620)
                     /locus_tag="P5549_01980"
     CDS             complement(34763..35620)
                     /locus_tag="P5549_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRW11874.1"
                     /translation="MNKRLRIEYAGAVYHVIQRGNNKEFVFHQAQDKGYFLKSVEKVM
                     RFCEADTFAYVIMGNHYHLLVRTGEIGLGQVMHRLNTRYSMYYNQTKNRSGHVFQGRY
                     QAGLIQDERHLLTVLRYIHQNPLRAGICDRVGEYRWSSDHCYRYLQPGFVNKEFVLDL
                     LASDQGKARNEYLHLMESVVEPENDYDDNFEKIPWIPDIKPAAPILSTSAAKQLKSLD
                     EILLAATIKRETFELIKAGSRRRELTPLKAIFIDLALQEGHPLQNIAAHINISVVAAF
                     KLKNVQNIP"
     gene            complement(35907..36629)
                     /locus_tag="P5549_01985"
     CDS             complement(35907..36629)
                     /locus_tag="P5549_01985"
                     /inference="COORDINATES: protein motif:HMM:NF022354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection system antitoxin AbiGi family
                     protein"
                     /protein_id="HRW11875.1"
                     /translation="MQSYFSKIYWHFTGGPKVDWENVTAPREIKHKTKSPAESVQILN
                     QIIAARKLLASCTERIMGGLTTREFCCVCDIPFKDLIGHAEYYGQVGIGFAARSIYPH
                     FNPVLYLERDFPAHGTIELDDPLLAETVQRCGGRCSLYDYMRYLLGLDQNAHHRDFLN
                     RYFGHMTQCIKFTRFSENDDETFYREREWRSISGDFHFAPEDVEAIIVPREFIPAVKI
                     QLANCDYKNDIAIIPFDLLEKV"
     gene            complement(36820..38508)
                     /locus_tag="P5549_01990"
     CDS             complement(36820..38508)
                     /locus_tag="P5549_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRW11876.1"
                     /translation="MLERVVITLSASEIIFLFIGGLGLVLFGLHFMSEGLQSAAGDKI
                     RKVLEKGTRTPLRGVLTGTLVTALIQSSSATTVLTVGLVNAQLLTLRQAIGVILGANL
                     GTTITAYLIGFNLYDYVLPIVALGTFLYLAPKQNYLKMIGQALLGFGILLFGLNLMGE
                     GMKPLTEVAFIMNLMAGIDNQSLTGLVMGIVFTVLVQSSGATIGVLQQLALNGVVTFH
                     QAVPILFGANLGTTITALLAGMGASVTAKRAALTHFLLNIFGLVVFLPLFLMGFFEKF
                     IVWLTNMILLIPGLGGTWAALNIKLQIAQTHGAYNLCNVIILIPLISALAYLVTQLIP
                     DRDEDADDPYKPKYIDHRLLNNPSVALAQAKRETMQMGLIAYNAFNNAVDYFNNPQEA
                     LLKKGEVLENAINRLEREITDYVVLASRKQLSKEESNSASIILQSLNDIERIGDHCDN
                     IIEQADYATRHQVQLSGEAREEMDNIISVTKDTILLSYRALEKQDKSVATQVLGFEEK
                     IDELQAAYRKNHIRRLNEGVCNGNNGAVFLDLLVNLERIGDHCRNIAEYVLEQR"
     gene            complement(38673..39860)
                     /gene="nirJ1"
                     /locus_tag="P5549_01995"
     CDS             complement(38673..39860)
                     /gene="nirJ1"
                     /locus_tag="P5549_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative heme d1 biosynthesis radical SAM
                     protein NirJ1"
                     /protein_id="HRW11877.1"
                     /translation="MISVTKLLFNDEYFGDSLRYSHDSRGAANGATSGSGPVVVWNST
                     RTCNLKCIHCYMDSDARKYQDELTTVEAKRFIDDLAEFRVPVLLFSGGEPLIRPDFFE
                     LAEYTAAHGIRPTLSTNGTLITREVAQRIKDIGVGYVGISLDGLREVNDKFRGRVGAF
                     QAAMEGIQNCVAVGQRVGLRFTINSHNLAELDNIFDFIEEEQIDRVCFYHLVYSGRGN
                     RMVEEDVTPQQSRQAMETIIRRALDFEARGLKKEILTVDNHCDGVYLYLRTLQENPAK
                     AQTIKDLISLNGGNRSGIAFGEVDPRGYVHPDQFTQHITFGNVQERKFGDIWTDTSHP
                     VLAGLKNRQPLLKGRCARCQYLDICNGNFRTRAEAVTGDFWESDPACYLTDEEIGLNK
                     ANA"
     gene            40281..41633
                     /locus_tag="P5549_02000"
     CDS             40281..41633
                     /locus_tag="P5549_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRW11878.1"
                     /translation="MSHAVDDFTKTYSQICYENSLIDNRLYAQYGVKRGLRDINGQGV
                     LTGITNISMIQSQEKVDGKLVPCDGKLFYRGYNILDLVRGFVSEKRLGFEEITYLLLF
                     GLLPTPEQLTEFNAILASCRSLPRNFVRDVVMKAPSKDVMNSLTKSILTLTSYDKKVF
                     DLSLPNVLRQCLTLISVFPMLSVYGYHAYNHYNCDESLYIHRPDPKLSTAENILRMLR
                     PDKKYTELEAIVLDTSLVLHMEHGGGNNSTFTTRVVSSSGSDTYSVIAAALSSLKGPK
                     HGGANNKVVEMVRDIKAHVKDVSDEDEVKRYLSKILNKQSFDHKGLIYGMGHAVYSIS
                     DPRALVFKGFVEKLANAKGRKSDFELYAMIEKTAPEVIAQERQIYKGVSANVDFYSGF
                     VYSMLDIPLELYTPLFAMARIVGWSAHRLEELINADKIIRPAYQSNVTFKDYVGLDAR
                     "
     gene            complement(41685..>44201)
                     /locus_tag="P5549_02005"
     CDS             complement(41685..>44201)
                     /locus_tag="P5549_02005"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11879.1"
                     /translation="SPDGQSSIEITFAINRKPVAEEIQPNQVLGRYEYRSEDDYDPAE
                     DFVAGKDTVIQVFLPDDVKAEDHFDATVEVYRDGNQVAALSNYRRDLANNALIFIPSS
                     RSSCGNWQAGIYKFVVKLGEKCEELTLDNVKFQKQRKLRVLAVPVKANYAGTIETPGD
                     QWKNGSSFMRRVYPVAHDDFTYRQGSLFDASDESYNITTDAGQLKLWQALNSLQSSSE
                     PYDQIIGFIEKGIRLPNNSMMQGYTYGAPSNIVVNSDQDMPATIAHEVAHNYSVGDEY
                     RGGSFNLGINSPPLGYRGTDWNNDSITVTANDPKVRPFPGASGVLISENLHPYDTGGR
                     GLLKDSIGFMGSGAAQSKNWINPSVWKQLFSSLAGSNTPASVSPSMVAQEVSEGIRVV
                     EANGWVSQAGAVEITLPWSSCYTTEPIVSNSGPYTIQAVDAAGNILAGNGFTPNFFVR
                     TNPPRELDPAPFVQVVVPYPAATAKFIIVDQSQTVLAEYAVSAHAPTVTVTSPTTGQN
                     LTGTGTITWDGEDADGDDLTYNVAYTPDGAEWELLAFGLEDEQWVQDFNLLPGGHQAQ
                     IRVTASDGINSSTPAVSGVFSVTLRAPQVSIEAPTAEASFSLTEGGVVLQGLAYDPQE
                     GQIYDDSRLVWTSDRVGELGRGPTVFTSALDEGRHVITLTAGGSVSGAGTYSDGASVT
                     AEAVTQAGYTFVNWTEGGSEVSQSASYTFSISADRDLTAHFQANVPGPTDECFIATAA
                     FGSKFTWPVKLLRHFRDQYLLTNSLGTAFVEFYYQNSPPIAAAIADSPALRVLVRLLL
                     APIILGVYLLEHPLLLSAALLGLLLTLRRRRPSANYQNIP"
BASE COUNT        10432 a        12203 c        10810 g        10456 t
ORIGIN      
        1 aataccggct ctatgtaaaa gaagaactgg gggccattat tatcaaaaca accccaccct
       61 atattatttt tgccatcaat gaaagcaatc tgacggcagc tttttgggat tatttaacat
      121 tgctccacaa tgaaacccgg atggataaaa aaaccgttat caaacagctg caaaaaatcg
      181 taaatgcatt aacgtaacag ccaacagagg gacgttcctt ttgttggcct cacgagaata
      241 tataagacca aaaaaagaac gtacccctgt tgggttttat tcaaaacgca gggcgtcgac
      301 cgggtttaaa ctagcagcct ttctggccgg caaaatgcca aaaatgaggc caattaccaa
      361 agagaatata aacgcgatga agacaatggg cagcgatatg gcagtattga taccggcaga
      421 ggaaatcagg ctacatcctc cccagcccaa aagcaccccg atgatgcccc ccatcccgct
      481 gaccaccgcg gcttcaatga taaactgggt caggatatcc cggcgccgag ccccaatggc
      541 cttgcgaata ccaatttccc gggtgcgttc tgtcactgat accagcatga tgttcatgat
      601 cccgattccg cccaccagca gtgaaatacc ggcaatcccg cccagcatca ggctcatggt
      661 ctgggagatg ctgctgacgg tttccaacat gtcgtcctga ctggaaaccc ggtaggcatc
      721 ttcgtcttgc ttgaattttt catatagctc attctcaatg gcggccacag cctgggtgga
      781 tacttccgca ttttccgccg agacatagac catggaaatc ccgctgctgt ttaaaaagcg
      841 ctgggcagtg gtgatgggaa tcaagatgat ctcgtcattg ctggcaccca tcgaactgcc
      901 gctttcatcc agcaccccga caacggtaaa atgagtgccg ttgatctgca ccgttttgcc
      961 aaccggattg gtcccggcgg aaaacagctc attgattata tccacgccca ggacggctga
     1021 cttctgacgg ttctccacat ccaggctgag aatgtccctc cctccctgga tggtgcggtt
     1081 tctgacgatt ttgtaatcac tgtccacccc catcagggag atgttttcca tttcattgtt
     1141 gccgtacttt atcgtcacac tgccggatgc aaccggcgca gaagcgatta taccgtccag
     1201 ttcagcaaat gctttggctt cttcataggt cagcgtcgtg gtcgcgcctc gccccatgat
     1261 gcgaacggtg atcagattgg aacccattga ttcgatcgat tccgtaatgg aagccgtcgt
     1321 tccctgtccg atggaaacca gaataataac tgcacacacc ccaataatga cgcccagcat
     1381 ggttaaaaaa gaccgtaatt tgttggccag gatgctgttg acggaaatct tgagcagcat
     1441 tttcagatcc actcaattca ccccccgatc aacgatcaag ccgtcacgga tactgacgaa
     1501 atgacgtgcc tgttgggcaa tatgcatatc gtgagtgatt agcacgatgg taatgccact
     1561 ctcattcaat tcacgcaagg cttgcatgaa tacctccccg gacttgctgt ccagcgcacc
     1621 ggtcggttca tcggccagaa taatcggcgg atcgccggcc aaagcccggg ccaccgccac
     1681 acgctgctgc tgcccgcccg acagttgtgt cggtttatgc ttcaaacggt cgcccaggcc
     1741 gacgcgatgc agggcagctt ctgccttctc acgccgttcc cggggaggca tcccccggta
     1801 gatgagcggc agttcaacgt tttcaaatgc agtcagcttg ggcagcaggt tgaactgctg
     1861 aaaaataaaa ccgattttgc ggttgcgaac ttgcgccagt tcatcttcgt cgagacaatc
     1921 aactgcctga ccgtcaagaa ggtattggcc ctcgtccggt acatccagac aaccaatcat
     1981 gttcatcaag gtcgatttgc cggaaccgct gggaccaatc accgcaatca tgtcaccggc
     2041 cggcacatgg agtgacaccc cttgcagcgc atgtacgatt tcttccccca tctggtaggt
     2101 tttaacaata ttctccaagc ggatcatgtt taaaatcctc ctgcgggcgg cggtccgccg
     2161 gttcttccgc ccgatccgcc catcatgccg ccttgctgac gctgctgcgt gatcgaagaa
     2221 gatgtcttgg cagtggcttg cagggcgtta tacactacat tatcaccttc ctgcaatcca
     2281 gctttgatct ccgccgtagt ggaagtgaca atccccacct tgacttcggt gcgtttctcc
     2341 tctccattgc tgcttcttat tttgacgaat tttttatcac cctgctcagt gatcgcatcc
     2401 agaggcaaca gcaaaacatc ttccttggca gcgagcagaa tttcggcatt gacattcatc
     2461 cctgctttta aagccccggg atcatccagc aatagcagca catcaaacgt tgatacacct
     2521 gaagaaacgg ttccttctcc ggcaatttca gttaccttgg cggtaaatac ctgccctttg
     2581 atggcgtcgc tgctaacccg ggccgattgt ccggttttga cgttaagaat atccagttca
     2641 tcgaccggga tggtcacttg taaagtgttc agacaggaaa tggtcaccag attggattga
     2701 ttgtccgtca cctcttcccc ctcggtcacg ttaaccgcta ccactgttcc ggcgatgggc
     2761 gcatacaggg tccttttctc cagttcccgg ttctgctgag ccaggctcag ttgtttttgc
     2821 tgaatggcga tgcgttgatt gctgatctgt tcgtccagat cactgctctc caattccgcc
     2881 aggatttgcc cctgctctac ccattgattg gcagttacgt ataatttggc cactgtaccg
     2941 gcgcttttag cctgcacatt ggcggccgaa ggccactcca gcgtcgtccc ctgaatcgcg
     3001 gcaatcgtac gcccggtggt aggtacctcg actttggtga ccgtcgttcc ggaagtcaac
     3061 cggcccggat tgctgaccgt aatcagcaca tcacaaatgc gattgccgcc agcggcatta
     3121 atggtgctca cgctggttac cgtgcctttg ataccggtca ggtaatccgc cacaaaaaga
     3181 tccgcttcct gaccggtgct gatgttcaat tccgtgccca aattgaagga agcccgtgcc
     3241 tgcatatggg tttgatcggt gatggtggaa aaagaagaac ctttgcccac attgtcgcct
     3301 accgccggca agctgtcacc cagcacgccg gaagccgtgg ccacgatctt taaatttttc
     3361 ttgtcttgct gcaagctggc catttcctgt tgggcctgct gcaaattcag ccgggccgac
     3421 tgcacctggc tgtcaaaatt atcattgtca aacgtcatca gcaaatcgcc tttttgcacc
     3481 tgttggcctt cgctcacata tagtttgcta atcgtcccgg tacattcgga agcaatttcc
     3541 ttgcggtcga tggcttcaat ggttcccgag ccactgatgg tttcctcaat attgcctttc
     3601 accaccgcca ccgatttcat tgtctgcaag gcgtcagcag tgctgttctt attttctgcc
     3661 gccgagtacc atagcgcagc cagcaatacg attgccaccg ccgcgataat caatctcttt
     3721 cggcctgctt tggcaacaat agccgcccag atttttttca aaagataccc gcctcctctg
     3781 tttgatctat ctctttcatc ttactgaccc ttggcctgcc aaaccgtgac tcaggaataa
     3841 acaggagtta ttgcatttct ttactatcat tatgccgctg ttgtatgaag gaatgatgaa
     3901 gagttccctt ttttcgtaaa aaacaaattc agtcaagaac tgataatttt ttctgtatta
     3961 aattgagaat attttgtcag ttaaaattta gatatgttag aataattatg taagcttata
     4021 agaatatctc tgcccaacat cttcctttaa gttctttgcc acaaaggagc gaatttgatt
     4081 tgttgcatac caaaatcatg gtcgtagatg attccaatgt cgatattgcc ctgattaaaa
     4141 gcatactgac tgattatgat ttgataaccg ccggggatgg cgtggaagcc atggaattgc
     4201 tgcaggaata ccccgatacc gaaatcatga ttttggattt gaacatgccg cgcatgaacg
     4261 gttttgaagt attggaagcc atgcgtaaac acccggttta caaaaaagtt accgttttaa
     4321 ttctcaccaa ctacgacgaa acggaaaatg aaatcaaagg gctggatatg ggcgcattgg
     4381 attatatccg caaaccaatc aatattcaat ctctgcgcaa aagaatacaa gtccataccc
     4441 atctgcgcag agcccgccag caactgcaac ggtataatga aatcttggaa aaaaccgtcc
     4501 aggaaagaac ccgggaattg gtaataaccc gtgatgtttc cattcatgca atggttatac
     4561 tattggagaa acgttccatc gagtccagcc atcatgccaa aaggacccaa tggattatga
     4621 aagccctgtg cgaacatctg agctcccagc ccatctatca aaacgtactg acggaagaat
     4681 acatcaaaga actttttgat acggcgccgt tgcatgatat cggcaaagtc ggcatacctg
     4741 acaggattct gttaaaaccc ggcccgttgg atgaacgtga attggcaatc atgaaaaagc
     4801 ataccacata cggcgtcgaa gccttgcagt tgggaaacga tgaaaacctg tctgtttcgt
     4861 tcatcaaaac cgccattgaa atcgccggca cccatcatga aaaatatgat ggcaccggtt
     4921 atccaaatca gttaaaaggc gaagagattc ctttgtcagg ccggctgatg gcggttattg
     4981 atgtatatga cgccatcatc agccaacgag tttacaaacc tccttatgaa cataccacgg
     5041 ccctggcata tctgcttgaa gaacgcggaa aatcatttga tcccaccatc gtggatgctt
     5101 ttttgcaaat cgaaaaaatg gttgaatata tcagcaaaac cttcaagcca tattaatata
     5161 ggggaggtat ctacccatga aaatgattcg gattttgatg attgccattc tgataacttt
     5221 tctttttccg atgatatcac tggcgtcctc tcccagtctc gacctgaccc cggaagaaaa
     5281 aacctttatt gcggaacatc cggaaattcg tctgggggta gacccgaaat tcatccccta
     5341 tgaatttatt gatacggatg gcgtttacaa aggaatcgct gccgattata tcaaactcat
     5401 cagccagcga accggcttaa cgatgacggt cgctcctgat ctgacctggc cggaagcata
     5461 cgaaaaagcg gtaatcagag aactggacgc attgccctgt atcgcccaaa ccagggaacg
     5521 ggaaaaacat ttcttgttta cccagcctta ttacaccttt cagcgcgtca tttatatcaa
     5581 ggaagacaac acttcaatca actccctcga tgatttaaaa gatgaaacca tagcggtgca
     5641 aaaagacagt tcccaccaca gtttcctgct cagctacccc ggactcaagc ttcatctgta
     5701 taccaatgtt gaagatgccc tgcgcgcggt agcttccggc gaggaaaccg cgttcatcgg
     5761 caatctggct acttccaatt acttgattaa atcctatggc ttcaccgatt taaaatacat
     5821 aaaaatggcc ggcgaagacg aaaaatccct atactttgcc gtacgcactg actggccgct
     5881 cctggtcagc attctcgaca aggcgttggc cagcattacg gaagaagaaa aactggcgat
     5941 caccgacaaa tggattggaa tcaatgaaaa tcccgattat ggcaggttga tcagaatggc
     6001 cggcatggcc ggggcggtta ttgccttggc cttaatggtt tcttttttct ggatcagacg
     6061 gctgcgcaag gaaatcaaga taagaaaaca gatccaggac gaattaaata ttgccaaaaa
     6121 tgaagcagaa tcagccaatc aggtcaagtc aatgttcctg gccagaatgt cccatgagat
     6181 cagaacccct ttgaacgcaa ttatcgggat ggcttatctg ctgagaaaaa ccgatatcaa
     6241 tgcgacccaa cgaaactatc tgcataaaat caccgaggcc tccaacaata tgctggcgat
     6301 catcaatgat attttggatt tctctaaaat tgaagccggg aaagtcgaaa tcgaaaacgt
     6361 accttttaac ctggacaatc ttctcagtga aatcattaac atcatctcct ttaaagtgga
     6421 agaacagggc atccatttta ccttggaaaa agatccggat atgccggaaa tatttaaggg
     6481 cgatcccacc cggatcgggc aggtattgtt gaatatcgtc aacaatgcgg ttaaatttac
     6541 gccgcaggga atggtgtcat tatccgttca tctagccaaa aaagaaatga ccacatgtag
     6601 actgcaattt gtggtcacag acaccggaat cggcatgtca gaagaacaac tcagccaaat
     6661 attcaaaccg tttgaccagg gagaatccag catcaacaga cgttttggcg gaaccggcct
     6721 gggtttgtcc attgccaaaa atctgctgga tttgatgggc ggtgagatta ccgttgaaag
     6781 tacgccggag caaggttccc gcttcactat tcaactggaa ttggaagctg aaccatcttg
     6841 cacgcgggaa gaaaaccgaa aaaagaactc cgtttttttc aagaacttgc atgccctggt
     6901 gatggagaaa agtgctatgg gcaccgaatt actgaaaaaa tatctgcatt cctttgccat
     6961 tgcagccgac ttcgctgcct cagacgcaga agcgatgcgc ttgatcgaac agtcaggcaa
     7021 tggcagcaaa ccaccctaca atctgtttat catcgacttt gaaaccccca aagaaggcgg
     7081 tatcggattt tttaacttct ggaaacaggc ctggtctgcg tcagaggaaa tgcccaaatg
     7141 catcatgatg gtccctttga cccgagccga tctgcacgag gcagctgaag ctgcgaaaat
     7201 agattttacc atcaacaaac cggttctgcc ttccgtcctc tataacgcca tcgtcgaaat
     7261 tttcaatccg gcaatgctcg aagcacaagg caagtctctg ctcacctccg accttgcaaa
     7321 gccggtgaca gattatccct atcatgtgct gatcgtggag gacaacaaaa ccaatcagtt
     7381 catcgccaaa accattctgg aacagtccgg attgcggact tcgattgctg aaaacggtca
     7441 ggaaggctat gattgtttcc tcgccctcca ggatgaactg gatgccattc tgatggattt
     7501 gcatatgcct gtactggacg gatttgaggc gactgcgctg atccggacaa aaaacaaaaa
     7561 gattccaatt atcgcgatga ccgccgatgc aattaccgga atcgaagaac aatgccgccg
     7621 cattggcatt gatcattatg tcagcaaacc ctttgatcca gatcaatttg tggcgacgat
     7681 catcgaagtg attcaatcac agccgcgcat ggtcgcggcg acggagaaga gtaagaaaca
     7741 aacagccgct gcccgggatg aagaagctgc cctggacgtt gctgccgggc tcaatctgct
     7801 gggcggaaaa gaagatctgt atgatatggt tttacagcag tatttacttg aaaacgaatc
     7861 agtcccgcag ctcctgcagg cagccatcgc caaactcgat tacaaaaatg ccgttcagat
     7921 cattcataaa atcaagggca gttccggcaa tatcggcgcc aaaacattac ataaaacggc
     7981 gcgggaattg caacaagcgc taagtgaaga gaatactgcg gaaatagacc gtctgcatca
     8041 tcgtttcctg caggaactgc agcagctcct gcagcaaata cagcaacgtc tgcaaaaata
     8101 aacaaccggg aggataaccg cccggctggc atttattcca agtctctggc ctgcgtctgg
     8161 caggattatt tatttctgat atacttgagc gtaatatgcg gacggctgga tttgcgtgat
     8221 tgaatctcaa actgcttcaa ggaagaaatc aagggcgtga gtttggaata gccgtagttg
     8281 cgggtatcaa agtccgaata gcgcttgttc agcttcttgc cgacttcacc caaaaacgcc
     8341 cagccatctt catcggaact ctcggtcaca atgcctttga tggcttcgat cagttcatcc
     8401 agactggcca tgctttcctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 tgaaaatgtc acgcttgatg taggcgtgtt gaggggtccc agcggcatca gcatgatcaa
     8581 aatgatcagc gacaaccccg tcttgggcga aggcgtgcag gtcaattatt ccattgaaca
     8641 gtcccccgac gtgttaaacg ccaagctgct ttcgggtgag atcgaactgg ctaccatccc
     8701 taccaatacc gcagccacac tgtacaataa aggggttccc tatcagttgg ccagcatgaa
     8761 cacctggggc gtaatgtatg tggtgagcaa cgatcaggcc atcacttcgt gggcagatct
     8821 caaagggcaa accattggcg cggttgccaa gggagcttct tcagatgtcg tcttcaaata
     8881 tttgctcagc aaaaacggtc tggatcccga caaagacgtg accctgaatt acaatcccag
     8941 tccggtggaa ctggcccagc tggtcatagc cggcaaggtt gccgttgctg ccctgcctga
     9001 gccctgggtc tcggtcgtcc tgaacaaaaa cccgcagatc aaggtcggct tggatctgca
     9061 gcaggaatgg cgcaagatag cgggagaaga tgtccccttc gcccagacct gtctggtcgt
     9121 aaaccgcgat tttgcagcgc agcatccgca aatcgtggag acattcctgg ctgaatacca
     9181 gcaggcgatc gattgggtca atgcccatcc ccagccggca gccgagctga tcaagaacac
     9241 cgacatcggc atcccggccg acgttgccgc ggatgccatt ccccgctgca acttaaagta
     9301 tatgggcagt caggcagcca agccggcggt gacgcaatat ctgaacgttc tgctgcagtt
     9361 ctcacctgaa acagtgggag gcaaaatgcc cgatgataaa ttcttctacc aacaataaaa
     9421 tcatcacggc tttatcgata ttggccatgc tcttgacctg gcatgggttg gcgctgctga
     9481 tcggcaagga atatattctg cccacaccgg cccaaaccgg gctgagcttg ctgctgatca
     9541 tgcaatcgca agacttctgg ccggcggtac tgatgaccgt tggccggggg ctgttgggat
     9601 ttttaatttc catgggactg gggattattc tgggcatggc ggccgggttc agccgggtaa
     9661 tcaactggct gctgcgaccc tgggtcacag tgatgcgaag caccccggtt atgtctgtca
     9721 ttattctggc catcatctgg tttgagtcca acctggtgcc ggtggtggtt gccattctca
     9781 tgcttttccc gatcatctac ggcaacgtgg tggcgggcat ccgcaacatc aatcaggaat
     9841 ggctggagat ggctcgcatg tataaagtga agccgcgtcg aatcatagcg gagctttatt
     9901 ttccggccat cctcccctat ctgctggccg gtgcgacaac ggccatgggc attgcctgga
     9961 aagtcattat tgccgccgag atcctcagcc agcccacccg ggcaatcggc accgacctga
    10021 tgatcgccaa attcaatttt gcgacggccc aggtgctggc ttggacggtc gtcgtcatca
    10081 tgatcagctt tgtttttgaa tccttgctgg gcgcatggga aaacaatctg accaactgga
    10141 gaagataaga tggcttttgt attggacaaa atcaataaag ggtttggcgg tctcgaggtc
    10201 atcaaagacc tgagcatgca ggaagaagaa aaccggattg tgtgtatcct ggggccttcc
    10261 ggagctggaa aaacgacact tttaaacctc atgtccggag ttatgaaggc cgacagcggt
    10321 cagatatcag gtttttccgg gcacagcatc agctatttgt ttcaggaaac caggctgctg
    10381 ccgtggaaga cggtacggga gaatatcgat tttgtcctca aggacaagct gccccgtcac
    10441 caacgcgagc agacaattga gcgctatctg gaaatggtgg aattaagtcc ttttgcctcc
    10501 tattatccgg cccggctcag cggcggcatg aaacagcggg tctcgatcgc ccgcgccttt
    10561 gcttatccgg cggatgtctt gctgatggat gaacccttca agggactgga cacccagctg
    10621 aaaatgagcc tcatcaagtc attcatccgg ctctggaccc tggaccggcg cagcgtcttt
    10681 tttgttaccc atgatatcca ggaagcagtg ttgatcggcc atctggtgta tgtgctgacc
    10741 gatcgcccgg ccagcatcaa aggccggatc atcaatccgg ttccgcacga gcagcgcgat
    10801 gacagcaatg ccccggaatt acagtccatt gagcaggcca tctatcagct ggttactctt
    10861 taaaagcagc tttgccccca tcaatcctgc cggcgctgct gccaaccgtc ccgcccgcat
    10921 cgggcaccct tcgatttcgc ttttttaaat atgacaaata tcttccacga tctgcccgtc
    10981 acgcatgatc agcaagcggt ccgtgcgctc tgccagcgcg cggtcatgcg ttaccatgat
    11041 aaagctggtc ttgaactctc gattgatctc ccgcagcatg gcaaatacgt tttccgtatt
    11101 gctggtgtcc agattgccgg tcggctcatc agccagaatc atgcgcggct ggttgatcag
    11161 ggcacgggca atcgccgccc gctgctgttc cccgccggag atggcattgg cccgataatc
    11221 gcggcggtgg ctcagcccga cccgttccag caaatccagc gctctctttt ttaccgtcgg
    11281 ggttgcccgg ccggcataaa tcagatacgg aatcataaca ttttccaaca cggtaaactg
    11341 cggcagaaga aaatgcgatt gaaaaataaa ccccatggtt tgattgcgaa acaaagccag
    11401 gccgtcatca tccaacgttc ccaaatcggt gccgtcaatg atgactttcc cttctgtcgg
    11461 gcggtccagc gcaccgatga tgttcagcag cgtactcttg cctgagccgg aggccccgat
    11521 cagcgaagta aactctcccc gccggatctc cagatcaacc ccgaacagta cgcgggtctt
    11581 gactttgtcc ccgaaatccc gggtaatacc catagtttta atgagtacat cagttattgc
    11641 ggatcacctc gatcggatct atttgggaaa cctgacgcgc cggcagataa gccgccaaca
    11701 ctgccgccac aatcgtagac aagacaatga tggcggtcgt catcggtttc aacagtaatg
    11761 tgtaatcctg tttggccagg acgatgaagg ccttgctcat caacaagccc agtcccagcc
    11821 cggaaagcgt tcccagcgtg cccagcacca gaccctggat catgaacacc cgcgacacac
    11881 tgccggtacg gatccccatt gctttgagaa tgccgatctg tttggatttc tgtaccgcac
    11941 tgatggccag cacggaagcg acgcccaaaa taaccgccag catgacaaag aattgaatgg
    12001 tataactgga actgctctga ctgcgcagac cggccaaaag agaagcattg ctctcctgcc
    12061 agctttccac cgccaagccg ggcagacgtt gactccattc tgcggccagc gcatcggcct
    12121 gcagcacatc atggacctgc acttcaatcg tattgacccg gtcccccatg cccagcagcg
    12181 ccgccgcccg gcgctgatcc atgaccatcc agcgctggtt gatggcgctc acgcccagat
    12241 caaagatgcc atccaccacc accggcaatg aatccctgcc ggacagttcc agggtaatgg
    12301 gctcgcccac actgaccccg agatcagccg ccaaatcttt gccaatcaat acagccccat
    12361 ccggcaggtt gggactgcct gccaccagtg aaccggtcag attgtaaata cggtcagcct
    12421 ggttcagatc aaaacccttc aggagaatcg cccggctaat ctgaccgcgc tcgatcaagc
    12481 ccgacccttc caccaccggc agtgctgttt tgatccgttg gtcagcggtc agccccgcag
    12541 tgatattgcg ccattccacc atcggccgca tcgtcttgcg ggtggcatcc agcaccaaga
    12601 cggggttgcc gttaagggat ttgacagctt cggccgccgc attttcatcg ctgctgatca
    12661 caatatgcgg ggatcgcccc actgtttttt caatcaagtt ggcctgcaat ccgtcaatca
    12721 gcgccgacag gaaaaccatc accgaaaccc cgatggcaat ccccaggata atcagaaacg
    12781 tctgcatcct gttctcggtc aggaaacgac tggccagact gaattcaaac cggatactgt
    12841 tttttcgcat aagctatccc ttgcctgacc gaaccgagac agttgcacct tcgcgccaac
    12901 cgctcggttc cagcaccaga tcatccgcct taagtccctg cgtaatgatc accccatccc
    12961 cggtgcgcaa gccaatctgc accggcgcaa aatgcgcctg gctgtttacc gccagccaaa
    13021 ccccgctttg tcccgcctgc tcactcaaat aacgatccgg cagaatcacg gcatccgact
    13081 gcgttgcggc aaacaactgt accgacagca gctgtcccgg atagagatcg ggagtctcgc
    13141 cgacaaagtc cagctcggcg gtgaaggatc cctgttcggc attccccagg gggtcggtgt
    13201 agaccacctg cgcatcccat ttttttgatt ccgcattggt gatccaaacc ttggcctgat
    13261 tgcccaaggc cgccagcgcc gcgtaacgct gatccggctg gacctttatt ctgagcccct
    13321 gtccggctgc cagcaagact gcccggcttc ccttgctcag catttcgccc ggtttcacgt
    13381 acaaatccag caactgtccg tcggcaggcg ccacaatttt gtactcgttc aataaaatct
    13441 ctttttcctg cagatccagc tgacgctgct gtaattcggc ctgcaggata gccatggccg
    13501 aaccctggct ggccagtgat tccaattgcg cccgggccga cctggcagcc tcctgcttca
    13561 cctgcagctg atgcgcagcc tcttccaatt cggtctcact gaccgcaccg gaagcatgca
    13621 aagattttac ccgttcatac tgggcggcgg ccttctctgt ctccagatcc gcctgaatgc
    13681 tcctggcccg cgcctcttca cgggttaccg cggaggcctt ctgcaattgt gcccgggcca
    13741 tttggaccgc tgctgctgcc cggtcacgat ccaaatgtgc ctgggcatcg tcgatctgta
    13801 ccaagagttg tccatgccgg accaactccc ccttgccaat cggacaggcc agcaccttgc
    13861 ccgctgccgt cgccgacaac agcgtggagc cgtccgcaat cacctccccg ctcagcagca
    13921 gggaaggaac gtaatcctgt tgagtcactt tataagcatt gacctgtgtc ggtctcagcc
    13981 agacaaacag cagcagtgcc gctgccagaa gaatcgctgt gacaacgatg gtttttttat
    14041 gttgcagcat agcttgcgtt accctccacc ctttattttc accattataa ttgattgccg
    14101 cgatataaac ctactactaa tttttctcaa gccctgccgt gatgtaaatt gtcagaaaat
    14161 tcaataagtt aatgcgtcag gcgtattaac ttattgaatt ttcgaaattt tctggctcaa
    14221 tacgccaaaa aaaaatccca tcgtagtgat gggatattgc tgtctccacg gcaggtcccc
    14281 ttaaaaaagg gggatctgct gataggttgc aatcggacaa ctcctgtacg cccgaccacc
    14341 agttcatgca aacgcttgtg tcagtcaaac aaccttttgt acgttcccct gaccgtttac
    14401 tttgaacctg gatttattat aacacgaatc atgtgagatg caaagcgatt ccggtaaagt
    14461 tcttgtaaat tttttgtaat gcaaccaggt cgggaaaaac tttgcccttg cttttccctc
    14521 ggcattcacc ggcggacagc ccgctgttcg cttgtcaccg gaaaaagaat gcattaaact
    14581 gatatcagcc aaatgcaggt cggaggagga attattttga ccgccaaata cagctacaat
    14641 ccacgggatg ccaaattttt gctgaaagag tggcttcccc tggaagatat tttacaatac
    14701 gaacgctatc aggaactcag cctggatgac attgacggtc tgctggagca gatgaaccgc
    14761 atggtggcca aggtgatcgc ccccaccaat gatgacggcg agatcaacca tccccgcctc
    14821 gtcaacggac gggtggtggg accgcccacc tggaaggacg tgtatgtata cctgcagcag
    14881 cagggctggg gcaccggcaa ttttgaccgc cacgctgccg gcacgctgcc tgaagttgtc
    14941 tatgcggccc tggcagaaat gatgtgcgcc gccagtcccg cttttattta ctatccggcc
    15001 cttaccaccg gcgctttcaa agtcattcaa agctatgcca gtcccgcgct gcaggagata
    15061 tttctgcccg gtatgatgga cgggcgcttt cagggctgca tgtgcatcac cgagccttcc
    15121 gccggcaccg atgtcgggga cggactgacc cgggccctgc ccaccgatga gccgggcatc
    15181 tataaaatca aaggcagcaa aatctttatc agtgccgggg acggagacca cgccgaaaac
    15241 ttcatttatc tcaccctggc ccgcatcgcc ggcgccaaac ccggcaccaa aggattatcg
    15301 ctgttcatca ttccccgctt ctggatcaat gacgacggca gcaccacccc caacgacgtg
    15361 gagaccaccg gggtggaaaa caaatttggc attcgcggtt gtcccaccgt ttccctggcc
    15421 tacggcgaca atgaccagtg ccgcggctat ttagtcggcc atccacctga tgagcaaggc
    15481 aacggcgagg gcatggcgca gatgtttcag atgatgaacg gcaagcgcct ggaatgtggt
    15541 ctgacctccg ccggcgtgac cgccaatgaa tactggaacg ccgccgccta ttgccgcgaa
    15601 cgcatacagg gtagacccct caacaatccc cgcgccgacc gcgttccgat tatgcagcac
    15661 gaagatgtta aacgcatgct gatgctcaac aaagccacca ctgaggtcat tcgctccctg
    15721 gtgatgaagg cttacttcta tcaggacatc agcgactatg atcccgatcc ggatcggcgc
    15781 aagtgggcct tcgcccggct ggaatgcctg tttccgatat gcaaggccta caccagcgat
    15841 gaagcctgga ccttgatctg cgagtccatt cagatttacg gcggctacgg atttaccgaa
    15901 gactaccccg ccgcccgggc cgtacgcgat accaaaatca attccctcta cgagggcacc
    15961 aattacatcc agtccatgga cctgatcacc cgcaaatggc cgctgcacaa aggccaggcc
    16021 ttcgccggcc tgctgcagga tattgagtcc ttaataacca gcgaacgcgc agcttatccc
    16081 gagttggaac gcgagtttgc caaactggag aaagccctgc gtgcctatca ggaattgcag
    16141 ggcatcaccc tgggctattt ccagcagggc aagatcgagc tggtggccag cttcgcccgc
    16201 cgcatcctga ccgctaccgg acaattgctg ggcgcccact tcctgctcga gcaggccgtc
    16261 atcgcccaat ctcatctgcg ggagctggag aaagaccatt atgattacaa ctattatctg
    16321 ggcaagaccc tgtcggccag gttctatctc aaccaaatcc tgcccaacgt ctggaacacc
    16381 accgatctgc tccgcgaagg cgaccccacc gtcctccact gcccggacga gatcttcgcc
    16441 ttttaatact cgaaatcttt gctgaggtta aggcattgat cttttgcaga ggtttgtgct
    16501 taagataagc gccagggggt aaggcggcag ccgtgcgatg ggacgtaacg acgccaagaa
    16561 cgcccgcaaa gagaataaag atatacgtta cccaaaaacg agactcacgt aattgaataa
    16621 agacccctgg catgcagatg agcgccaatg ctgcaactct ggcaaatcaa gagaccggcc
    16681 ccaagcgttc ttaagtacaa acctcacgct aacgatacaa aaatagccan nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gtaccttgcg gtccggtttg cccaccgaag
    16861 tccgcgggat ttcgctgata aattccacca gcgtcggcac cttgtagggc gccagactct
    16921 tgcggcagtg ttcaatgacc tcttccgcgg tcagcacttc gcccggtttg accaccacaa
    16981 acgccttggc cgtttcaccc cgtttgggat ccgccacccc caccgtgcag gcctccatga
    17041 ttttaggatg ggcatacagc acttcatcaa tatcacgcgg ataaacattg aagccgctgc
    17101 acagaatcat gtcctttttg cggtcaacaa tgaaaacaaa cccgtcttcg tcgatcctga
    17161 caatgtcgcc ggtatacaac cagccgtccc gcagaacgtt gtcagtttcc tcctgattgt
    17221 tccagtattc ccgcataatc tgcggcccgc ggccaatcaa ttcacccagt tcattgcggg
    17281 ggatgacctg cgtgccggta tcgacatcga ccaccactag atccgtatcc ggccagacaa
    17341 tcccgcagct gcccggtttt ctcagggtaa acaccggggt taccgtcagg atgttgaccg
    17401 tctctgacat gccgtagcct tccaggatgc gtccgccggt cagccgttca aactcgttca
    17461 tcacttctac cgccagcggc gccgacccgc aaaagatgcc tttcagcgag gtaatgtcag
    17521 cactggcaat tttgggatga ttgttgagac cgataatcat ggcggggacc gccgcccaga
    17581 tgttgggacg gtggcgttcg atggcttcca gcagattgtc cggggtgggc agcgccacca
    17641 ggacaatact cccgcccgta aacaagcaca cgttgacgtt ggcattgaag ccatatatat
    17701 gattgagcgg aatcgcacaa agagtacgaa cctcttcctt ggacaccagc ttgaaggcgc
    17761 ctgacgtcca ggtggcggtg cggttggcct gggagaatat tatggcattg gtcaggacac
    17821 agcccttggg aatgccggtg gtgccgccgg tatattgcaa gcgggctacg tcatcggcaa
    17881 atacttcgat gtccggttcg gcatcactgg ccgccatggc cagctggttg aaatcataaa
    17941 tcccttctcc gggaggaatt tccactgccg cgctgggcac ctggaaaacg atcacccgct
    18001 tgaccggaga ggcagcatct tgcatgattt gaatggcctt gtcggcaaag gcggccatca
    18061 cgatgacggt ctccgtgccg ctgtctttaa actgcagcgc cagttcggga atcgtataca
    18121 aagggttggt aggaacttca atggcgccga tcttgaaaag cgcctgcagg gatagaatgt
    18181 actgcggaac attgggagcc atgattgcca cccggtctcc tttttgcacc cccaggccca
    18241 gcagggcgtt ggcgattctg cgggccaggt tgttggccgt gccgtaattg atggcgacat
    18301 cacccattaa tatatagggc ttttcaggcg tttcttccgc ccatttgttg aaccagtact
    18361 tcaagggata atcaggatac tcaaaattct tcggtacacc ttcgtcgtaa tgtttttccc
    18421 acagggggcg ccatttggct acatctgctt caaattcctt cagccacggt ttctccattg
    18481 ccatccctcc gtatcgtttt attcattttt gtaatattaa cacttattag acaaatgcat
    18541 aaagaattcc tgccaggaca tgaccttcac aagcgaatta ttttctcttc tctgtttcca
    18601 tgcaaaacgc catcttaaac gccggttctt ttataaattc tccctgtgat caagaaatat
    18661 acctgccctc cggcaaagga tagctatatc taaacgttac ggagttgtca ggttgaaata
    18721 agaccgtcgg caagagggtt taagcaaaaa aaggtgcaag ctccgcggcc gcacccttga
    18781 ttttttctga aaatgtttta ttcggaacaa cttccccgaa cgtcgcagca attggtttga
    18841 agtgcatatt ttaaaacagg ttatcgcgct tctccttgct gctgtgcctg ctcttgagca
    18901 gcatgaaacc gcctgcacgg ccatatctca ccgcctgaat ctcagcgacc accgacaagg
    18961 cgatttccac cgccttttgt ccgcccagat ccaggcctat cggcgcgtga atccgttcca
    19021 gccgttcatc ttggatcccc agggcggtta gttttccaaa gtaggcaacg acctgctttc
    19081 tgttgctgag gactccgata tacctggccg gagaagcaat aacggttttc agcaccgcct
    19141 cgtcgaagtg atgacgatgg gtcacaatca caatgctgtc ggtggacctg atctcgtagc
    19201 tgctgatatt ctctacaatg tcaccgccga tccgttcgct ggccagcgga aacctgtccg
    19261 gggtgagcct ctcctcctgg ttgtcgaaga tgacgatttc ataatccaga aaactggcaa
    19321 actggtatac ataaaaagcc acattgccgc tgccggtaat gatgattctg tccctgtctt
    19381 ccatactctt tccccgcttt cacctgtcgt cgcgccattc tttccattcg ggcctgcgcc
    19441 ttcccggagg aaaccatcag gttggcatct gcgctgcaag cagcgccggc atctttgcaa
    19501 aacttgacac gctggccgtc ctgtccggaa aagttgttga tgaacaatgt ttatgccggt
    19561 tggcagcaat tcatacctgc gccggctctc ctcacggccg atggaactca gcaatataag
    19621 ggaggttgcg gtactcctcg cggtagtcca ggccgtagcc gaccaggaag agatcgggca
    19681 tggtaaaaca gcgataatcg attttaacgg tgacaatccg cccagctgga ttgtccagca
    19741 gggtacagat tttgaaactg gccggcccgg cattctcaat gtgttggatc acataaccca
    19801 gcgtcagccc ggtgccgatc acgtcttcca ccagcagcac atgccggtct ttgagattga
    19861 tatccaaatc cttggtaaaa tgaaccccgc cgctgtgatt ctctgatggc ggggacgccg
    19921 cgatcgccaa aaaatcaata ctgagcggga tgtgcagttg ccgggtcaga tccgccagaa
    19981 aatagatgga acctttcagc acactgatga tgaccagttt ttgcccggca tagtcgcggt
    20041 tgatttcctc cgccacttcc cgcagccggg tctggatttg ctcctgtgta taaatcattc
    20101 gccccagacc gggagccggc atcaatcatc ctcccctttc cgcaaaccga accgctctgc
    20161 cagatgatag aaatcgtttt taaagacaat attgatattg atgtccggat acagcttttg
    20221 cagcatccgc acctttttct ttttttgcga aacatacttt tgatccatgg tagtcagctc
    20281 aatataggta tcaaaacgcg gcagataaaa atccggcgca aacgcctggg tcacattgcc
    20341 ctccgtatcc cactgcaccg gaaaggtccg cggttcatac tcccactgga tatgatgcat
    20401 gtccaggatg gtggcaaatt ccgcttcaga aggatgtttg aaaaccacct ggcgaacgct
    20461 gtcctcgtct gcagccggtg ccggcagcgc tgtccccagt tgcaagtgac gggccataaa
    20521 ggccagcact tcagccccct cttcctcact gatacagtcg gtgttgagca tcagatgata
    20581 aagggtggga tccgcccaat cgcgctcata aagggtagcg atatagcgcc ggtgtttgcg
    20641 gtcggtcaat tccaggaagc gctgcgcctc tttttctccg atatgaaggc tctgcatcag
    20701 ccgccggttt ctgatctgcg gggatgccat gattttgaca tgcagaacgc ccggctgtcc
    20761 ggcaaatatc atctgggcgc cgagcccaaa cacaatcgcg gagtgctgcg cggcatacgc
    20821 tttgaggcgg ttttccagat aggcggcata ggtgatcccc tccgccgacg tattcagaaa
    20881 atagcgcgga ctctcagaca gcatatgcag ctcatgtttg tcagcgattt ccgggaacca
    20941 ttgattcatg accaggtccc tggtgatgac cggcagatcc agtttcatcg ccaggagatc
    21001 ggtgatttcc cggcccagac tgccggtttc ccgggaaacg gtaatcagca tcgcacccac
    21061 tcctgccctg tatttttgaa aggcggcagc acatcttccg gcacctcaag tcatcttcat
    21121 tattgctctg ttgactttta tccctctgga atgctggctg cgcctcatcc tggcaccttt
    21181 tcttatactt gaattatagc atgaaattat acttgatttc gcagcttgtg atatagtcct
    21241 ggttcgtccg gagcaaaata ttttgacgcg aaatcaaaaa ccttaaaaat ttttaaaatt
    21301 agacgattga gcgagataaa tcatccaaac agctcatcag gttaaatttt cagaaaattc
    21361 acgcgttttt gctgtttgct cttgttgtca tcgtcctgta tgatgtaatc aagctccaag
    21421 gaattcctaa gacggaaacc tatccgagcc ggaaacagga aacgaaacac gaaaactgaa
    21481 tataaggcta gtgaaagcgg atttcactat atcgaaacgg aatcagggaa aatggtttgt
    21541 caccgggatt gtaaaacgga agttcaggat tgaagatctg caaaggtttg aaggcaggaa
    21601 cagaagtcag gcaaagccag tacagacgag gagtaactga aacccgcaag acgaaggcct
    21661 tataggaaag tgtaagtggg aaacgaaatg gcaaaggcaa agacaggtgg aaatcgataa
    21721 aggttcgttg gagcttttat tacggcaggc accccgctag cgaggcgcct gccgtttgat
    21781 atggctttgt ttttcgccag caatgaccag catgactgcc agccccagca gcgtcccgcc
    21841 caggtcaaga cccagatcaa ccatttcaaa ggcccggccg gccacgaaca actgatgcag
    21901 ttcgtccgtc agggcgtagc ccatgcacag gagcagcgtg agagcggctt gccgggccag
    21961 acgccacgga tattgccgca acgcgatcat gcaaagcatg gccaggacaa agtagctgcc
    22021 gaaatgggcg gcattcctga ccaaatgctg cagactggcg acccattggg cctgctgcgc
    22081 cgccgtcatc tgcaggaatt ccggccggat aaagaccagc agcgtgcgga tcatttctcc
    22141 gctcaagcgg gtcgattcat cagccgtttg cgaagaaaag aaaaacagcg atccggtcca
    22201 gatgagaacc ggcagccaaa aaaacaactt cttatatcga atcattatac tatcctttta
    22261 tacagttcca gtaccggaat cggcaccagc gccagtccca gaccgaccag atacagggaa
    22321 gacggcagtt ggaccatccc aaaagcatcc gccaccggag gaataaacag gaccagacag
    22381 atcaggatca gcgaggccag cgctgccaga ttcaaatacc ggttggtaaa gaatccggtc
    22441 gtaaacaagg aatgcgggga acgcatatta aacgcatgga agatttggga gccggccagc
    22501 acgataaacg ccatggtacg ggcggcctcg atgtttccgg tgctcttcca gacactcata
    22561 aaaccgatca gggtcagcaa ggcaaacatg gtgccttgca gcacaatgcg caagccaaat
    22621 ccgccggcaa agattgattc ccctttgggt ttgggccggc ggcgcatgac gtcctcttcc
    22681 accggttcca tgcccagcgc gatcgccggc agactgtccg tcaccagatt aatccacaac
    22741 agctgcaccg ccaggaacgg tgacacctgc cagaccagca tggcgataaa caccaccagc
    22801 acttcgccga tattggttcc cagcaggaag cccacggtat gtttgatatt gtcataaatg
    22861 ccccgccctt cgcggatggc atcgacaatg gtagcaaaat tgtcatcggt caggatcatg
    22921 tcagacgcgc ctttggccac atccgtgccg gtaattccca tcgcacagcc gatatcagct
    22981 gctttcaggg ccggcgcatc gttgacacca tccccggtca tggaaactac cgcgtcttgc
    23041 tgctgccagg cccggacgat gcggatcttg ttttccgggg agacccgggc atagaccgag
    23101 atatgatcaa cccgccgctg cagttcctct tccggcatag catccagggc cgcaccggta
    23161 accgcctcat atccttcctg catgataccc agatcccggg cgatcgcagc cgctgtcacc
    23221 acatggtccc cggtaatcat aaccggtttg atcccagccg cgcggcacac cgccaccgca
    23281 tcccgcgctt ccggccgggg cgggtcaatc atacccagca gtcccataaa agtcagaccg
    23341 ttttcgagta ccaccggggt aatcgtttcc ggcaaagcgt cgatagcttt ccaggccact
    23401 gccagcaccc gcagcgcctg actgctcaat tcatcggtca tctgccgtcc ggcttccaca
    23461 ttgccggcca ggcagcgctc cgccagaaca tcaaacgcgc ctttgacaat caccacatat
    23521 ttaccgtcta tttgattgac ggtggtcatc agtttacggt cggaatcaaa cggcagctca
    23581 cactgccgcg gatacagacg gttcagttca tccttgggca gaccgttttt atacgcggcc
    23641 agcacgatgg ccgtttcagt tggatcaccg atatgggttt cccgaccgtt ctcgtctatt
    23701 tccacactgc cgtccgagca gagcgtcgca tactgcagca gttttttgat gggcgcggaa
    23761 ttgtctgcat caatgctttc ggtcagtatt tcggcggcgc tgaaggcctt gaccagggtc
    23821 atctggttga gggtcagcgt ccccgtcttg tctgaacaaa tcaccgaagc gctgcccagc
    23881 gtctctaccg ccggcagctt gcgaatgatg gcatttttct taaccatgcg ctgcacgccg
    23941 atggccagca ccactgtcac gatggccggc aagccttcgg gaatggcgga tacggccagc
    24001 gcaaccgcca ccataaagat ttccatcatc ggcatgccgc tggtgagccc gatcacgaaa
    24061 atcaccgcgc agattgccag ggcgaagaaa ccaatgtatt tgcccatttc cgccagcttg
    24121 tgctgcagcg gcgtctgggt gtcctctgcc gtgctgagca ggttggcgat tttacccatt
    24181 tccgtattca tccccgtggc cgcgatcact gcccggcctg tcccgtaggt gatgctgcag
    24241 ccggaataga ccatgttgag gcggtcaccc agcggtgcat cagcgtttac gatgacaccc
    24301 gcatcctttt ccactgggac agactcaccg gtcagcgctg attcctctga tttcaaacta
    24361 ttgctctcaa tcagcctggc atcggcggga acgaaatccc ctgcctccag tcggatcata
    24421 tcccccggca ccagcgtcgc tgcatcaatc atcatttccc ggccgtcacg ggttacccgc
    24481 gcctgcggcg ccgacagatc tttcagcgcc tccagcgctt tttccgcctt gccctcctgg
    24541 accacgccga tcaccgcatt cagcaccacg atcaacagga tcaggaaagg ctcgaaatac
    24601 ccttcccctt catacatggc aaccacgaaa gaaatcaagg cagcgatcag caaaataatg
    24661 atcattacgt ctttaaattg ctccagaaag agctgcagcg ttgttttctt ttggccttcg
    24721 gccagtttgt tgggaccctg ttcagccagt cgccgggcag cctcttcgct gctcagtccg
    24781 ttggcgggat cgacacccaa ttcctgcagg acggattgat cgtcccgatt gtatggcaat
    24841 tgaatcataa aacctcctga ttccgggcat atgcgataag gcatatttcc cagccgcaac
    24901 caatttcttt tcattattcc cggaatcaac tgtcaggtca agtttttgtt tctgataacc
    24961 cccccgtagg cgggagttgt ttttttatct gtgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnncgtcggg gatggtaata tcatattcag cgtttggttt tgctgccgtt
    25141 tttcgtcttt gatgatggga ttgaaatccc gccttgggat ccacaaaaac aggagaaatg
    25201 cttgttatgg atgaaaattt gctgatttac gtgttcgatc cgcaggatca attggtttat
    25261 ctgcgggacg tctttaaaaa gaagcttccc ctgtcccatg ctttgctggc ccgcctcaag
    25321 acccaggaaa agatccatgt caatggtatg tttgctcata ccaattaccg cctgcagccc
    25381 ggcgatgtgg ttaccgtgga tttgaatctg gcggaaacca atccgatttt gccgcaggac
    25441 atccccctcg agatcgtgta tcaggatgcc gatgtcatgg tcatcaacaa accggccggc
    25501 atggccgtcc atcccgtcaa agcaaaagcg gaaggcaccc tggccaatgc cgttacccat
    25561 tactggcagc agcagggcag gttctgcctg tttcgccccg tcaaccgcct cgacaaaggc
    25621 acctccggtc tggtgctggt ggcccaaagc cagtatgccc atcaagccct gttccgacag
    25681 cagaaactcg gtcagatcca gcgccgctat ttggccgtgg tggaaggcgt ggtggaaaaa
    25741 gaccaggaac gcattgaact gcccatcggc cgcagccaac cgggcagttc tgtctttcgt
    25801 caggtcgatg ctgccggcaa gccggccgtg acccattata ccgttatcca aagatatccc
    25861 cggcacacgc tgctcagcct gcgtctggaa accggccgca cccaccaaat ccgcgtccat
    25921 ctggcccatc tcggccatcc cgtgtgcggc gactccgtct atggccaggc ctccccgctc
    25981 atcgcccgac aggccttgca cgccgcctgg acctcattcc tccagccccg cacccaaacc
    26041 cgcctcgagt ttgcagtccc cctgccccca gacatccagc atctcctcac cctcatctaa
    26101 ttgtccgaat attccataag ttaatacgtc aggcgtatca acttatggaa ttttctgata
    26161 atttatcaga agttgctttt ttgattggcg gtggtcaatg ttcgcaccag tacggcggcg
    26221 gcttcgccgc gggtggtgtg atgagcggcg ttaaagttga ccccgtcgcc tttgataatt
    26281 cccagccccc agcataaggc ggcatagcct tgcagattcg catccatact gttgaaatcc
    26341 cggaaaggtg cttgcagagc cggggacggc atgttggcga taaagtcgac atccagcatc
    26401 cgcaccatca gttgcgccag caatccggtg gtcatggggg tttggggatc cattttttcc
    26461 ttgagccatt ggctgcgcag agcctggtca atgatttcct catcgctgcc gcggacgggc
    26521 tcataatagc catttcgcgc catcaccatg acccgcagca tggtcaccag attgatggtt
    26581 tcgtgggggt ggaaagttgt accgtattcc gtcatgatac ccgcctgccc gagcatggag
    26641 atttcctttt cggcaaaatg accggcgata tccgtgaatg cctgcggctg caaagcttct
    26701 gccaccggtg ttccgtccca gctcaagggt tcgccgctga ccgcgtcaaa catgttgctt
    26761 gactggtttt gcacctcggc ccggggaatg taaaccagct tgtattcccc cgaagactca
    26821 ttgcgggtga gcaaattcgg gtaggtgagc tcatagcgca tggtcagggg ctgatgctgc
    26881 aaatatacct gattggcttt ttccatgttc atcacctggg ccggatcgga aaatgtcagc
    26941 gtcggccatt ccaaacgata ggaagagacc tgtttgctgc gaccgtcaac ggcgatgcta
    27001 atcccctggt tcaggcaggg aatgctgttc tccagccggg tgaattggaa ctgccattga
    27061 gcgggataag gtgattcctt gacatatatt tcttgataaa catctttctc caggcgcacc
    27121 gagttttgtt ttgaaggctg gatgcgggcc aggaagtcac gggcgattct ttctgcggca
    27181 gccttgtcca gcatcggttt gacgtcattc aagtcgcccc ggtcaatata aaaattgcga
    27241 atctcgccgc tcacggcatc aacctgcgcg ctgacataag cagaactgtt gacctccgta
    27301 tcgttgcgcc agttgaggtt ccaggtgcgc ttttccggat cctgatagtc tttgtataga
    27361 ttggcttctt ccagtttgac attggccggt aagctcaccc actgtttgac catggaaacg
    27421 gccttctcct gggagatcaa attgagcgtg cgctcgactt ccgcctgttc ctgcgggctc
    27481 aaatcagcct ggggggccgg gcttttttcc atggaaccca tgccgccgac atcgtttatg
    27541 ccgtaaatgc tttgattctc gatcggctgt ccgctggccg catcaatcat tccgccggag
    27601 ttgtgcatca ggcgatacac cagcagcggc tgggccttct cgtcaccctc gcgcaggatc
    27661 gaatatttgg gcgcgatata atattgcagc tccagcattt tgttggccgc aaacacctcg
    27721 gccgcttttt ctgccgagat gaattccgtt ttcgcgggga taattttgtc tgaccagttc
    27781 aggtcgtagc tgttgacggt gccatcgccg gtgctgatct ggagggtgac tccctcgttg
    27841 cccaccggga tttggttggc catccgctgc cagcgcagtt catactgtcc cccctgccag
    27901 ctgcttaagg aaatgatctg ctggttgggc acatattgca gctcggcaac ccggtcggga
    27961 atcaactttt tcagcagctc caggccgatc tgccgggcct gatcgataga aacagcggga
    28021 atctcagtgg tctgacgccc ttgtttgtag atatgcatgg aggtaatcaa ccccgccgtg
    28081 gcatcgacct cagcgttgaa cgaccccagc cggttctcct tgtcggtcca gctcaatgac
    28141 cagatttgga tattgtcccg actgcggaag ctggacgtaa attcggtata ctccgccggt
    28201 atggtgaaat tttgtttcac cttttggatg gcctgttcca gagaaagcgc cgcttccttt
    28261 tccgccgcca tgaccccggc cggcagcagg gacaacagca gcaggaccat catcagcaaa
    28321 ctcataccct tgcgcaatcg cttcttcatc ctcacactct cctgtctttt attgatggtt
    28381 gttttccatc ccggagaccc ctgtctcccg ggttccgcct gcagagaggc ggcggcctgt
    28441 cgaattgaca ttttgcagct gccaaaatga tcttctattt gcatttacgc attacggata
    28501 tgtagcttac accggggaaa gaataagatc gaccaaattt tctatttccg gcaacgggcg
    28561 aacgctttta gaactaactc cttaagcgac agatataaaa acgggagcag tttttttatc
    28621 tgctcccgct cgggttgaac ctggcgagtg ttgcatcaat gttccgaaaa atatgctcga
    28681 tcaattttac catttctttt tctatcgcat ccacagggca tcgctgtgga gcgaccccaa
    28741 tgcttttcgt gctacaacgc agcctggttt gacgatgcag tttgcgcccg atcaagcccg
    28801 gaaacgattg ccctgccatc gtccgcagtg ggtgcttccc cacccccaca caccatgaaa
    28861 acagcggtaa tttggctaga cgcaccggtt ggctagacgc cttcgaagcc gcccgccttc
    28921 agctttcttt gaaaatacag gaaaaccggg aacggaataa tgatccagcc caggctcttg
    28981 atcaggctgt ttttggcccg gtcgatctgc ctttgttttt ccgccacgac catggcatca
    29041 tagttggcct tgagctgttc ctccgttaat ttgctgacct ccgtcgtcac tccgccttcc
    29101 ttgaccattc tttccgtact gtataacttg taatcctcaa aagtctgatg ataggcagtc
    29161 ggagcaacga tgtcggcggc ggccataaaa gctgccacgc ccccgccgat ggtcatcatc
    29221 aaggttgcaa acagcactag ataaacatac accttcttga tcatatcttc gcctccattt
    29281 ttcgattcgc cgcgcacacc gacgatgaac agaacgacca gcgccacgac acctaataaa
    29341 accagcagtg ataccgccat actcatagga cacctctatt ccgtataaga tctgtacaac
    29401 aacgatatat gctgattata tcccataact tgcacccaca acaaaaataa gtcaagcacc
    29461 aggccgctca cctcgctaaa tgttcagaat attccataag ttaatgcgtc aggcgtatta
    29521 acttatggaa tattctgaat tttgtggata aaaatccagc cgggaaaata cattcccggc
    29581 tggaaaaaga gaggggttat ggaagacgaa agggccttca gactgtcact atttatccca
    29641 tttaccttcg gaacggagtc tggcaactac tttttcggcc agttcgatca gggcggggac
    29701 gttgactttt ccgacccggg ttgccgggag gggttcgtcc accggccaca gttcaacgtg
    29761 aatcggccgc ttgtacgcag ccatgttttt gcaatactca aggatctctc ccggggtgag
    29821 ttccacaccg ggtttgggct gcacataagc aaacacaccg tccacgaaca cttcatgtgg
    29881 cattcccagc acctgggcct gggccacctt gggatgccgg gagatatagt cggtaacttc
    29941 gtcagggaac accaggaagc ccttgggttt gatgacaaac ttccggcgcc cggcaaactt
    30001 cagtcccata tagctgccga aatcatgaaa atagcccatg tctccggtat agaggatgcc
    30061 ttctttggag atggcagcag cagtcgctgc cgggttattg tagtaaccca ggaatacgat
    30121 aggcccatgc acgcaaatct ccccgacttc tccggggggc atttcctcac cggcggtgcc
    30181 gtcctcattc atgggctgcc ggatggtaac tggcgcatat tccggaaaca cctgtccgac
    30241 ctgaccggcc atttcttcca gggaaatgcc gcggggggtg gcggtaaaat agccggcaca
    30301 ctcggtcatt cccaaagatg tcccgaaagt cgcagccatc gtcgacatct tctccaggaa
    30361 gggtacgtcc accgcactgc cgccgtagag aatatagcgc agactgctca gatcatagct
    30421 ggcatagttg ggatcgagcc acagcatgcg gaacatggtg ggcacgcctc cccaccacgt
    30481 gggacggtac ttctggatgc agcccagcac cgcctggggg ttgtacatac taagggaaac
    30541 caccgtgccg cccacgaacc agaccgtcat cggtccctgg caggtgcccg ccacatggct
    30601 ggtaggcatg gaattcagat accggctgtc cgtcccgtac aagccaactt cacgcgagaa
    30661 taccgcgttg ttggtgatgg tattttcatg acaaatcagc gccggtttgg gctccccggt
    30721 tgaaccggtg gtaaaaataa taatgtgggc atcgcgcttt cccaactgat cgtattcctg
    30781 gcgcagtgac tcgatcaaag cgctgtcctg ttttaattcc tccagcgccg cgggtgcgaa
    30841 atattcgtca aaattcaggc agccctcgag aaaatctcct tctttgccgc cgggggtata
    30901 ttgtatataa tgtttgaccg aaggaaccgc ctgctgcact tccttgacca aatcgcgaaa
    30961 atcacgaata ggcgtgttgc ccagacagaa gaaggccacc gggttgatct tgctcatgtc
    31021 acgaacgact tcatgtccca tcagacggac atccagcggg gtcatcaccg cgccgacggt
    31081 tgcgcaggca taagtcagca tgtaaaactc cggccaggcc agccattggg ccaccacgat
    31141 gtcgcctttt ttaatcccca ttttcttcag acgcaacgca tacaaggtga tcatctcgtc
    31201 aaactcttgg taggtatagc ttttgccggt atcggcacta accagggcgg gattgtcagg
    31261 ggtaatggcg gcccatttct taatataatc agtcagcagc ggcagcttcc atttctccat
    31321 tagattttcc tgcaacggga ttatgctcat cgttcatcat cctttctgtt ttggcttgtt
    31381 acttactgtt agataaacta gcaaaaacta taccagatgc ccgcagaaaa acctgtccgc
    31441 aataaaaagc cgacaggttg ggaatgtttg gcttggacgc tttattgttg gcacggtcca
    31501 gggccttgtt caattgtaaa ccactgttca tagtttgaac atttatgaac aacactaaac
    31561 agtctggtca tctactttgt acttgctcat ttttttgtaa agggtggtgc gggacatccc
    31621 catttcccga gcgatccggc tgatatttcc gttataaagc tgcagcaatt ccaagatccg
    31681 ggcgcgttcc cggtcctcct gttgctgtct accggcctgt ctggcatccc aaatgctttt
    31741 tccctccccg tcttcctgcg caggcatgct cttctggaca ggggtttcgc gatgcagtaa
    31801 tttggcagcc aacgtcccgg aaggcaattc catatcgctg agccgtcctc ccctggcagc
    31861 gagcagcatg ctttccgcca cgttctgcag ttccctgacg ttgccgggcc agtcatacgc
    31921 ttccagatgc cccattattt tcggcgccag gcagtcccga tcattttccc ggaaagcatc
    31981 attgctgcgg agaaaatgat ggaacaacag ccggatatcc tcacggcgtt cccgcagcgg
    32041 cgggatgcga atggaaatca cattgaggcg gtaatacaaa tcctggcgga aacgtccgtg
    32101 catcatttcg gtatgcagat ccttgttggt agcacagata atgcgcacat ccaccgggat
    32161 ggcctggttg ccgccaatcc gggcgatgct cttgtcctgg atcacccgca gcagggccgc
    32221 ttgctgctca aacggcatat cgccaatctc gtcgagaaag atggtgcccc ccgaggccag
    32281 ttcaaatttc cccgggttgc cgcccttgcg ggcaccggtg aaggcaccct cagcatagcc
    32341 gaacagttca ctgccaatca gttcccgcgg gatggccccg cagttcaagg cgataaacgg
    32401 acccttgctg cgtctggaat aattatggat tgattgggca aacaactcct tgccggtccc
    32461 gctctctcct tcgatcagca cattgctggt gctggccgca gccattttgg cattttcaat
    32521 cagatccagc atagccccgt taccggtaat gatatcttca aagcggaatg ttgccttggc
    32581 tccgctgaaa cggttgacga gctggcgaat ttccttgatg gggcgcagaa tcagcagacc
    32641 gctgacgacc accccagcct cattgacgat gggtgtgccg gatgccagga aatgcacctg
    32701 atcgcccggc gtgccataga tgacctcttc gtcctggaac gaaatgcgtt tgtcaaacag
    32761 atcattaatg aagtgacttt tgcccgtccg gacatctttt tgcagttttg tccggatttt
    32821 ttccagccag tcaccccaat tctgtttaaa cacctggttt aacagctcga tttcgccctt
    32881 gcggttgacc actaccaccc cgtctgacat gttggtgaca acattgttga ggttgcggtt
    32941 cagcagagtc agttcattgt ttttcaggct cagttcctcg gtacgctggc gcacccgcag
    33001 ttccagttca tcccgcgcgg cttgcagctg ttcttttaaa agcttttttt cagtaatgtc
    33061 ataggcgccc acgatgccca ggatttcgcc tttcatacgc gtcgtggcga aaaagacgtc
    33121 cacccagatg gcctcgccgt ttttcttgac aatcttgaat tcataattgg aggtgacctc
    33181 cttgccctgc aagcgggcca aacccctttc ccgaatcagg tcacgataat ccgggtggca
    33241 gatatcccag aagtcctttt gcgtcatttc ttcaaatgta aagcccagtg tcttctccac
    33301 tgccgggttc acatagatga cccgactatt gcggtaaaca tagaccagca ccgggatgtt
    33361 ttccgtcagt tgacggtagg agtcagcatc attgatataa ttcttgattt cctcttccca
    33421 tgaacgtttt tcccggtccg ttgctctatt gctttttcca gccatttcat ccacccccgc
    33481 gcaaatcttc tcctaacaaa gattttacac gaagcaataa ataacgcaaa cgtcaggccg
    33541 ctcacctcgc cgattattca gaaaattcca taagttaata cgcctgacgt ataaacttat
    33601 ggaattttct gaacattttt gagcttattg gtcaggcact tgcatttttt tattgaacgt
    33661 atatgctctg atattgctgc tgcgctgcct ccaactgagg tatgccgcga tcacatctta
    33721 aacttagcaa ggagcagtgt ttctcacatg catcacagaa caagctgccc agatactcat
    33781 tcagcgaagg tttatcgttg ccgttgtcgg aagcgatatt gctgtccgtt gatgtttcgt
    33841 tgttctgatc ctgctggtgc aaactatcac cgctgcccgg aactgcaatt ttatcggcgt
    33901 ttgacgtcga gcccggggaa cttttctgat catagtaggc atactcttgc tgcgcgattc
    33961 ggccatgacc atgtccgcgc cggggacgat ccgtataaat ttcatcctgc ttttgcacag
    34021 attgccgggc gatcatgtca tttgattctg atgccctgtt atcggaggac acatctttgg
    34081 aaaccacagc gatctgggta tataacaaac ttagaatcaa catgaccgcc gccgtgctca
    34141 gcgctgctct gccccatgcc gatcggtccg acgctctgaa cagtctgggt atgtttttgg
    34201 caataaactg ccagtgcaat gcaatatgca gaccaaacaa accgacacat gcataggaaa
    34261 cccatttatg cacgtcgatc agcgtatgac tgatcatctc atccgcaggg aacagcacca
    34321 tggaaatctg cacgccggta acgataatca acgccaccgc cagcaaagat accgtgttga
    34381 gcagataaaa gagcttgggt ttggccttta gacgggggtt gaacagattt ttgccaatgc
    34441 ttttgatcca tgaccaattg agtacaatat ggtaaataag caaagcaccc attgccaacc
    34501 cagcaatttc gtgaaaggta aagccggtgt tctcggcata cagcaaggca ataaacaata
    34561 tggtcagaac gatatctacg atcagcctga tacggtttcg ttcctgcatt tgaattcctc
    34621 cacctcagat tttactttca cctgtcgtta ttctacctgg tgaacatgaa aatcttgtga
    34681 acaaatcttt aagatattgt ggagataatg aaactcgtta tgttcagaat attccataag
    34741 tttatgcgtc aggcgtatta acttatggaa tattctgaac atttttgagc ttgaaggcgg
    34801 cgacgacgga gatgttgata tgggcggcga tgttttgcag cgggtgccct tcctgaagag
    34861 ccaaatcgat aaagatagct tttagcggag tcaattcccg tctgcgagaa cctgccttga
    34921 tcagctcgaa tgtttcccgt ttgatcgtag cggctagaag gatttcgtcg agagacttga
    34981 gctgtttggc tgccgacgtt gatagaatcg gggcggcggg ttttatgtct ggaatccagg
    35041 ggattttctc gaaattgtca tcgtaatcat tttccggctc aacgacgctt tccatcaggt
    35101 gcaaatactc attgcgggcc ttaccttggt cagaagccag taaatccagg acaaactcct
    35161 tgttgacaaa gcccggttgc aggtagcgat aacagtgatc actactccaa cggtattcgc
    35221 cgacccggtc acaaatcccg gcccgcagcg ggttttgatg aatataacgc agcaccgtga
    35281 gcaggtgtcg ttcatcctga atcaacccgg cttgataacg accctgaaaa acatgcccgc
    35341 tgcggttttt ggtttggttg taatacatgc tgtatcgggt attcaaccgg tgcatcacct
    35401 gccccaggcc gatttctccc gtccgcacca ggagatggta gtgattgccc atgataacgt
    35461 aagcaaaggt atccgcttca cagaaccgca ttaccttttc cacgctcttc agaaaatacc
    35521 ccttgtcctg ggcttggtga aaaacaaatt ccttattgtt accccgctga atcacgtgat
    35581 aaaccgcccc cgcgtattcg atccgcaacc tcttgttcat cctcgctccc cctgtgcaat
    35641 aaaataacgc aaacgccagg cacccgtcgc acattttcag aattttccat aagttaatgc
    35701 gtcaggcgta ttaacttatt gaatattctg aattcgccag ccggtagccc gcccgttgaa
    35761 ttttgtctcc atttattcca ttttatcaca tttatagcag aatggtgttg ctggtttttt
    35821 taaactcgaa aacgcaagcg tgaggccgca ttattttcgg aatattccat aagtttatac
    35881 gtcaggcgta ttaacttatg gaatattcag actttttcca ggagatcgaa cgggatgatg
    35941 gcgatgtcgt ttttataatc gcagttggcc agttggattt taacggcggg gatgaattcg
    36001 cggggaacga tgatggcttc cacgtcctcc ggcgcaaaat gaaaatcccc gctgatgctg
    36061 cgccattccc tttcacggta gaaggtttca tcgtcatttt cgctgaagcg ggtgaacttg
    36121 atacactggg tcatgtgacc gaagtagcgg ttgagaaagt cgcggtggtg ggcgttctgg
    36181 tccaatccca gcagatagcg catgtaatcg tacaaggaac agcgcccgcc gcagcgttgg
    36241 accgtctccg ccaaaagcgg gtcatcgagc tcaatcgttc cgtgggcagg aaaatcgcgc
    36301 tccaaataca agaccgggtt gaaatgaggg taaatcgacc gggccgcaaa gccgataccc
    36361 acctggccgt agtattctgc atgcccaatc agatctttga atggaatgtc acaaacacaa
    36421 caaaattcgc gggtggtgag cccgcccata attctttccg tgcaggaggc gagcagtttg
    36481 cgcgcggcaa taatctggtt aagaatctgc accgattcag ccggcgactt ggttttatgc
    36541 ttgatctccc tgggcgcggt gacgttttcc caatcgactt tcgggccgcc cgtgaaatgc
    36601 cagtagattt ttgaaaaata gctctgcatc ggacccattc ccccttgttg tacgggatta
    36661 gtgtccaata aaaccgcacc gcttatacaa aaaattccgc caaatcctgt ctttttggcg
    36721 gaacgaaatg ttcagaaaat tccataagtt aatacgcctg acgcattaac ttttggaata
    36781 ttccgaattt ggtgcgtcag gcgcttgacg ttgttgggat cagcgttgtt cgaggacgta
    36841 ttcggcaatg ttgcggcagt ggtcgccgat gcgttccagg ttgaccagta aatcgaggaa
    36901 gacggcgccg ttgttgccgt tgcagacgcc ttcgttcagc cggcggatgt ggtttttgcg
    36961 ataggcggcc tgcaattcgt cgattttttc ttcgaagccg agcacctggg tggctaccga
    37021 tttgtcctgc ttttccagcg cccggtagga cagcagaatg gtatccttgg tcaccgagat
    37081 gatattgtcc atttcctcgc gggcctcgcc ggataattgt acctgatgtc tggtcgcgta
    37141 atcggcctgt tcaatgatat tgtcgcaatg gtcgccgatc cgttcgatat cgttcaagga
    37201 ctgcagaata atggaggcgc tgttggattc ctctttcgac agctgcttgc gcgaggccag
    37261 cacgacataa tccgtaatct cgcgctcgag ccggttgatg gcattttcca acacttcccc
    37321 cttcttcagc aacgcttcct ggggattgtt gaaatagtca accgcgttgt taaaagcatt
    37381 atacgcaatc agccccatct gcatggtttc ccgcttagcc tgggccagcg ccacggaggg
    37441 attgttgagc agacggtgat cgatgtattt gggtttgtaa ggatcatccg cgtcctcgtc
    37501 gcgatccgga atcagctggg taaccagata agccagcgcg gaaatcagcg ggatcaggat
    37561 gattacgttg caaagattgt aagccccgtg ggtctgggcg atttgcagtt tgatattcaa
    37621 ggccgcccag gtgccgccca gcccgggaat cagcagaatc atattggtca gccagacaat
    37681 gaatttttca aaaaatccca tcaaaaacag aggtaggaaa accaccaagc cgaaaatgtt
    37741 cagcaaaaag tgagtcaggg ccgctctttt cgcggttaca ctggccccca tgccagccag
    37801 caaggcggta atggtggtac ccaaattggc accgaaaagg atcggtactg cctgatgaaa
    37861 ggtcaccacg ccattcagcg ccaattgctg cagcacgccg atggtcgcgc cgctactctg
    37921 caccagcacg gtaaagacga ttcccatcac cagcccggtc agggattggt tgtcgatccc
    37981 tgccatcaga ttcatgataa aggcaacctc cgtcagcggc ttcataccct cgcccatcag
    38041 gttcaggccg aacagcagga tgccaaagcc cagcagggcc tgtccgatca ttttgagata
    38101 attctgcttg ggcgccagat acaaaaacgt tcccaaggcg acaatcggca ggacataatc
    38161 atagagattg aaaccaatca ggtaagccgt aattgtcgtg cccaggttgg cccccaagat
    38221 aacgccaatc gcctggcgca gcgtcagcaa ctgggcgttg accagcccta cggtgagaac
    38281 cgtggtggcg ctgctgcttt ggatcaaggc agtgaccaac gtcccggtca gcaccccccg
    38341 cagcggcgtg cgagtcccct tctcgagaac cttccttatt ttatcaccag ccgccgactg
    38401 cagaccttcg gacataaaat gcagcccaaa cagcaccaga cccagaccgc cgataaacaa
    38461 aaatatgatt tctgatgcag ataacgttat aaccaccctc tcgagcatat ttcactttat
    38521 ccctagtata actgaacaag aaaaagttaa caaaacaaat aaaacgcgcg agcgcgttgg
    38581 ccgctttgca ttttcagaat attccataag tttatacgcc tgacgtatta acttatggaa
    38641 ttttctaaat attcaatatg tttgttcgtc cgtcaggcgt tggctttatt caggccgatt
    38701 tcctcgtcgg tcaggtagca ggccggatca gattcccaga agtcgccggt gacggcctcc
    38761 gcccgggtgc gaaagttgcc gttgcaaatg tccaggtact ggcacctggc gcagcgcccc
    38821 ttgagcagcg gctggcggtt tttcaagccg gccagaaccg gatgggaggt gtccgtccag
    38881 atgtcaccga acttgcgttc ctgcacgttg ccaaacgtaa tgtgctgggt gaattgatcc
    38941 ggatgaacgt aacccctggg atcaacctcg ccgaacgcga tgccggaacg attgccgccg
    39001 ttcaggctga tcagatcttt gatcgtttgc gccttggcgg gattttcctg cagcgtgcgc
    39061 agataaagat aaacgccgtc gcagtggttg tcgacagtca ggatttcctt tttcaggccg
    39121 cgcgcttcaa aatccagcgc ccggcggatg atggtttcca tcgcctgacg ggactgctgc
    39181 ggggtgacgt cctcctcgac catgcggttg ccccgcccgg agtaaaccag atggtaaaaa
    39241 cagacccggt ctatctgctc ttcctcgata aaatcaaata tattgtccaa ttcggccaga
    39301 ttgtgactat taatggtaaa acgcagtccc acccgctggc caaccgccac gcagttctgg
    39361 atgccctcca tggccgcctg aaaagccccg acccggccgc gaaatttatc attgacctca
    39421 cgcagaccgt caagcgaaat gcccacataa cccacgccga tatccttgat gcgctgcgcc
    39481 acctcgcggg tgatgagcgt gccgttggtc gacagcgttg gccggatgcc atgcgccgcg
    39541 gtatattcgg ccagttcgaa aaagtccggt cgaatcaacg gctccccgcc ggaaaacagc
    39601 agcaccggca cccgaaattc agccaagtcg tcgataaaac gtttggcctc caccgtcgtc
    39661 aattcgtcct ggtatttgcg ggcatccgag tccatgtaac aatggatgca ctttaagttg
    39721 caggtgcggg tggagttcca aaccaccacc gggcccgagc cgctggtcgc cccgttggcc
    39781 gccccccggg aatcatggct gtagcgcagg gaatccccga aatactcgtc attaaataaa
    39841 agtttggtta cactgatcat tgcccttccc ctttaaaaca ggtatttttc atctgcctgt
    39901 attatatcgc atttgcgccg gggacagcaa aaaactcaaa taaaccaaga cgcccaacac
    39961 ttttgatatt ttcagaatat ttaataagtt aatacgcctg acgcattaac ttattaaata
    40021 ttcagaacat tccataagtt tatgcgtcag gcgtattaac ttatggaatg ttctgaatat
    40081 ttggcggggg ggtggctgtg ttgttgaatt ctgaatttat taatgcctag atatgggcat
    40141 tacgatataa tagaagatat catccggtgt tgttgcggcg taatgagttg cagccatttg
    40201 cagttgcagt caaacgaaag cggcgccttt catcggttca ccgacaatat tgaaaaaaac
    40261 cattattaag gagtggattt atgagtcatg cagtagatga tttcacgaaa acatacagcc
    40321 aaatttgtta tgaaaacagt ttgattgaca accgcctgta tgcccaatac ggcgtcaagc
    40381 gtgggctgcg ggatatcaac ggccaggggg tgctgaccgg cattaccaac atttccatga
    40441 ttcaatcgca ggaaaaggtc gacggcaagt tggtcccctg tgacggcaag ctgttttatc
    40501 gcggctacaa tattcttgat ttggtgcggg ggttcgtcag cgaaaagcgc ctggggtttg
    40561 aagaaatcac ctatttgctg ctctttggcc tgctccccac cccggaacag ctcaccgaat
    40621 tcaatgcaat cctggccagc tgccgcagcc tgcccaggaa tttcgtccgc gacgtggtca
    40681 tgaaggcgcc cagcaaggac gtcatgaatt ccctgaccaa aagcatcctc accctgactt
    40741 cctatgacaa aaaagtgttc gatttgtcgc tgcccaacgt gctgcgccaa tgcctgacgc
    40801 tgatcagcgt tttccccatg ctgtcggtct acggctacca cgcatacaac cattacaatt
    40861 gcgacgaaag tctgtacatt caccggcccg acccgaagct atcgacggcg gagaacattt
    40921 tacgcatgtt gcgccccgac aaaaagtata ctgagctgga ggcgatcgtg ctggatacct
    40981 cgctggtcct gcacatggaa cacggtggtg gcaacaattc cacctttacg acccgggtgg
    41041 tgtcttcgtc ggggtcggac acgtattcgg tcattgccgc ggccttgtca tcactgaaag
    41101 gtcccaagca cggcggcgcc aacaataaag tggtggaaat ggtgcgggac atcaaagccc
    41161 atgtaaagga tgtcagcgac gaagacgaag tcaagcgcta tctgagcaag atcctgaaca
    41221 agcagtcgtt tgaccataaa ggcctcattt acggcatggg acacgcggtg tattccatct
    41281 ccgatccgcg cgcgctggta ttcaaaggat tcgtggaaaa actggcgaac gccaagggca
    41341 gaaaatccga tttcgaattg tacgccatga tcgaaaaaac cgctcccgag gtcatcgccc
    41401 aggaacggca aatctacaaa ggcgtcagcg ccaacgttga tttttacagc ggttttgttt
    41461 acagcatgct ggacattccc ctggagctct acacacccct ctttgccatg gcccggatcg
    41521 tcggctggag cgcccatcgc ctggaagagc tgatcaacgc cgacaaaatc atccgcccgg
    41581 cctatcaaag caacgtcacc ttcaaagatt acgtcggcct cgatgcccgg tgatttttta
    41641 aattgtgcga atattccata agtttatgcg tcaggcgtat taacttatgg aatattctga
    41701 taattggcgg agggacggcg gcggcgcagc gtgagcagca agcccagcag agcggcgctc
    41761 agcaacaggg ggtgctccag taaataaacc cctagaataa ttggggccag caacaggcgc
    41821 accaggactc tgagggccgg gctgtcagca atcgcagcgg cgatgggggg agaattttga
    41881 tagtaaaatt ctacaaacgc tgtccccagg gagttggtca gcagatactg atcgcggaaa
    41941 tgacgcagca gcttcaccgg ccaggtaaat ttggagccga aggcagcagt ggcgatgaag
    42001 cattcgtcgg tggggcccgg tacattcgcc tgaaaatggg cggtcaaatc tctgtcggcg
    42061 ctgatactga acgtatagct ggcgctctgg ctgacctcgc tgcccccttc ggtccagttg
    42121 acaaacgtat aaccggcctg ggtcaccgcc tcagccgtca cactggcacc atcactatat
    42181 gttccggcgc cgctgaccga gccgcccgcc gtcagggtga tcacatgccg gccttcgtcc
    42241 agtgcacttg taaacaccgt cggcccacgg cccagctcac ccacccggtc ggacgtccaa
    42301 accagccggc tatcgtcata aatctgccct tcctgcggat cataggccaa tccctgtaag
    42361 acgaccccgc cttcggtcaa cgaaaaactg gcttctgcgg tcggcgcttc gatcgaaacc
    42421 tgcggagctc tcagcgtcac tgagaaaacc ccgctgacag ccggcgtgct gctgttgatg
    42481 ccatcactgg cagtcactct gatttgggct tgatggccgc ccggcagcag attgaaatcc
    42541 tgcacccatt gttcatcctc caggccgaac gccagcagtt cccattcagc cccgtccggg
    42601 gtgtaggcaa cattgtaggt cagatcatcc ccgtccgcat cctcgccgtc ccaggtaata
    42661 gtgccggtcc cggtgaggtt ttgtccggtg gtggggctgg tcaccgtaac cgttggcgca
    42721 tgggcactga ccgcgtactc cgccagaacg gtttggctct gatcgacaat gataaacttg
    42781 gcagtagccg caggataagg caccacgact tgcacaaacg gggccgggtc aagctcccgt
    42841 ggcggattgg tgcggacaaa gaaatttggg gtaaacccgt tgccggccaa tatattgccg
    42901 gcggcgtcga cagcctgaat cgtatagggg ccgctattgc tcacaatcgg ttcagtcgta
    42961 tagcagctgc tccagggcag cgtaatttcc accgctccgg cctggctgac ccagccattc
    43021 gcctccacta cccgaatccc ctcgctgact tcctgagcaa ccatgctcgg gctgactgat
    43081 gccggcgtgt ttgaacccgc cagcgaagaa aataattgtt tccacacact ggggttgatc
    43141 cagttcttgc tttgcgccgc accgctgccc ataaacccaa tcgagtcttt cagcaaaccg
    43201 cgcccgccgg tatcataagg atgcagattt tcggagatca aaacaccaga tgcgcccggg
    43261 aaaggtctca ctttgggatc attggcagtc actgtaatac tatcattatt ccagtccgtg
    43321 ccccggtagc ccaacggcgg cgagttgata cccaggttaa aggagccgcc ccggtattcg
    43381 tcgcctacac tatagttatg ggcaacttca tgggcgatcg tggccggcat atcctgatcc
    43441 gaattgacca cgatattcga gggtgcacca taggtatagc cctgcatcat cgagttatta
    43501 ggcagcctga tccccttttc aatgaaaccg atgatctggt cgtagggctc actgctgctc
    43561 tgcagtgagt tcagggcctg ccagagcttc aactgcccgg cgtcggtggt aatattataa
    43621 ctctcatcac tggcatcaaa aagactgccc tgccggtagg tgaaatcatc atgggccacc
    43681 ggataaaccc ggcgcatgaa ggaactcccg ttcttccact ggtcaccggg cgtttcaatg
    43741 gtacccgcat agttggcttt gaccggcacg gccagcaccc ggagcttgcg ttgtttctga
    43801 aacttgacat tgtccagggt caattcctcg catttttccc ccagcttgac gacaaactta
    43861 taaatgcccg cctgccaatt gccgcagctg gaacggctgg acggaatgaa aatcagggcg
    43921 ttgttggcaa ggtcccggcg ataattgctg agcgcagcca cctggttgcc atcgcggtag
    43981 acctccacgg ttgcatcgaa atgatcttcg gccttgacat catccggcag gaagacttga
    44041 atcacggtat ccttgccggc cacaaagtct tcagccgggt catagtcgtc ctcgctgcgg
    44101 tactcatagc gtcccagcac ctggttgggc tggatttcct ccgctacggg cttgcggttg
    44161 atcgcaaagg tgatttcaat ggaggactgg ccatcgggag a
//
LOCUS       DAOTHZ010000008        44093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_880_length_44093_cov_7.437622, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000008 DAOTHZ010000000
VERSION     DAOTHZ010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 44093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44093
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_44093_cov_7.437622"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="murC"
                     /locus_tag="P5549_02010"
     CDS             <1..1182
                     /gene="murC"
                     /locus_tag="P5549_02010"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRW11880.1"
                     /translation="IQEGVDLLVISSAVPPENIEVKTAIQKGIPVLKRGEMLARLANA
                     SKGIAVAGAHGKTTTTSMVYTVLEQCGVQPTYIAGGELQDSALNAHLGQGEYFVVEAD
                     ESDASFLALQPYISIVTNVENDHLDFYQSFDRIKAAFQKFLQQTDNDGFVLLNGADPC
                     LQEISCSAAGRVLLYGEDSSFDYYLQDWQVQGMGSVFEAYRAGQHLGKITLSVPGRHN
                     AWNALAALAVAIELGHPFAAVAASLKDFHGAKRRFQIMGHIGNSVVVDDYAHHPTEIQ
                     ATLKAARDLDFARLLVIFQPHRYTRTHLLADQFAVSFSAADLVIITDIYSAGEKPIEG
                     VDSHLICRLAAQAGANIKYASTLEEAESAIRQEVKDHDLIMTMGAGDIWKLGVKLVDE
                     K"
     gene            1223..2470
                     /gene="murA"
                     /locus_tag="P5549_02015"
     CDS             1223..2470
                     /gene="murA"
                     /locus_tag="P5549_02015"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRW11881.1"
                     /translation="MEEHLLVRGGKALKGQVSISGSKNAILPIMAASLLSTGEVILNE
                     VPDLEDINVMAQALKILGAEIKREGKNLIIIGSGVNNCELPESVSRRMRASNLVMGAS
                     LGRLREARVAFPGGCAIGSRPMDLHIKGFKGLGYEISEGSGYLLGKAKHAQGKEIMLD
                     VPSVGATENIMMAAVLTPGYTVIRNAAREPEIVDLQNFMNRMGARVKGAGLDTIRVEG
                     VEKLGGVEHTVIPDRIEAGTFMVAAAISRGDVWIENVVVEHLQPVISKLTEVGVEILP
                     WAGGLRLIGNKDYRPTDIKTMPYPGYPTDMQAQMMSLLATVPGTSVMVETIFENRFMH
                     VQELRRMGADIKIEGRVAIIKGGQYEGATVESTDLRAGAALIIAGLAAHGETKIGKLE
                     HIDRGYEKIHEKFQNLGAEITRI"
     gene            2730..3458
                     /locus_tag="P5549_02020"
     CDS             2730..3458
                     /locus_tag="P5549_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRW11882.1"
                     /translation="MAKKAGKMSLLLLLVMVCIYLFLHSSVFNISKVYVSGQKKVTSD
                     EVKALAGVTPGENLFKIDGDLIEKSVEIHPVVKNAQMIRHLPHSVEIKIEERVSWAVV
                     PYEDLFLLIDDEGHCLDKLNKLPDGNTPIITMEQMPARVNLGQAVNPEVIKMIKTVWK
                     SIDAEGRAQISQFHYANTQKSLLIYTLEGTEVRYGNLERVEEKAANFTEALKIEASLQ
                     KEGKDQLEYVDLRFSGRPVVKTKT"
     gene            3472..4173
                     /locus_tag="P5549_02025"
     CDS             3472..4173
                     /locus_tag="P5549_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HRW11883.1"
                     /translation="MRSKTGQVSIMLVCLVLGIMLAVQFRNTQNTDVNLRGARTDEVY
                     VRLMEVTEERDALAKEVLSLREKMVNVSNNNDANRELQEEMQKANMAAGLLPVHGQGI
                     VVTLNDSARTVQIGEDPNGLLVHDVDILNVVSELKASGAEAISVNEERIIAMSEVRCA
                     GTTILVNTNKIAPPFIIKAIGDPQMLESGLTIKGGVAQKLKDYGLQVQIQKMDDVEVP
                     AYTGVIKTKFSQLKK"
     gene            4271..4606
                     /locus_tag="P5549_02030"
     CDS             4271..4606
                     /locus_tag="P5549_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HRW11884.1"
                     /translation="MWLLTLFCLLIGLFIGFQIPILLPAVFVKYMSIGVLAALDSVFG
                     GIRAYMEDSFDSTIFISGFITNTLLAGGLAYLGDRLGLELYLAAVVVFGVRLFQNLGV
                     IRRYLLKKY"
     gene            4701..5915
                     /gene="ftsA"
                     /locus_tag="P5549_02035"
     CDS             4701..5915
                     /gene="ftsA"
                     /locus_tag="P5549_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRW11885.1"
                     /translation="MVVGLDVGTSSVKAALAEVVSPGAVNILGIIHQPSAGLRKGNIV
                     DIEATAQSIDSCLNELERLTGVEIQSAFCGFSGMSVSAVSNHAVIAVANPNDEITEED
                     KRRVLQLAQNVALPPDKMIVQLIEQQYIVDGYDGVKDPVGMMGSRLEVEVMIIIAAAA
                     ALQNLQRCADRINLHTERFVYSPILMEEAVLMPAEKEMGVALVDLGGGTTEISVFEQG
                     VLLSTSVLPIGGEYITRDLAIVLRTSIEEAARIKEESGVADISMASSEVMINVRNIQG
                     SEVKQVSQHVIADIVAARVAEIATMVHAELMQTTDLDKLPGGLVLCGGGAQLKGIAEV
                     MEAYLNIPVRIGRPDNIRGLQREYNLPGNAVVLGCINYASRRASFSLIDHNHKFGKAF
                     GRITYWLKDLFG"
     gene            5935..7005
                     /gene="ftsZ"
                     /locus_tag="P5549_02040"
     CDS             5935..7005
                     /gene="ftsZ"
                     /locus_tag="P5549_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRW11886.1"
                     /translation="MPLDFDVEMQQFADIKVIGVGGGGNNAINRMIEAGLKGVEFISV
                     NTDSQALFLSKAEKKIQVGEKLTKGLGAGADPEIGMKAAQESEDDILRALQGADMVFV
                     TAGMGGGTGTGGAPIIARLAKQVGALTVGVVTKPFSFEGRKRNQQAETGIQELKNEVD
                     SLITIPNDRLLQVVDKNTAFNDAFRIADDILRQGVQGISDLIAVPGVINCDFADVKTV
                     MKNTGSALMGIGRASGENRSAEAARMAVSSPLLETSIEGARGVLFNISGGSNLTLFEI
                     NEAAEIIHQSADVEANIIFGANINEELADEVMVTVIATGFDAKKPLLSEPSLRSARPV
                     ESAQSILDKHDLDIPPFMRRSR"
     gene            7269..8147
                     /locus_tag="P5549_02045"
     CDS             7269..8147
                     /locus_tag="P5549_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HRW11887.1"
                     /translation="MSDNKVYADLTFLLNVSMDFLLLWLTARLCHLSIAYRRLLLAAC
                     LGGFYGVGQLISAGSILYTLPAKLVVSCLLLLVACPSKAWAQFGKAFMVFYGLSFITA
                     GASLAAASLVPTGEDASWSMYYLVAGVSALISIAIFAERFFRQTLLPVLLKYQVRLCF
                     DNKCCRGQGFLDTGNGLRDPLTHRPIVVAEFALVQSCLPEEICCAMEDDLNEEQRLEA
                     LSHSQWGNRVRLIPFSSLGKKNGMLIGLRCDELGIRVGQREMLHKNLVVAIYPYKLSA
                     NGDYQLLIPSEVVQQA"
     gene            8161..8883
                     /gene="sigE"
                     /locus_tag="P5549_02050"
     CDS             8161..8883
                     /gene="sigE"
                     /locus_tag="P5549_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HRW11888.1"
                     /translation="MNLREKWETLKLRLLKWYIKRKLPGYIHYIGSAEVLPPPLKESE
                     ESNLISRLDSGDGAVKSTLIEHNLRLVVYIAKKFENTGINIEDLISIGTIGLIKAVNT
                     FEPSKKIKLATYASRCIENEILMYLRRNLKNRAEVSLDEPLNVDWDGNELLLSDVLGT
                     EADMIYKTIEGEVEKSLLQQAMHKLNPREKIIIQLRFGLNNGYEKTQKEVADILGISQ
                     SYISRLEKRILKRLQREIRKIE"
     gene            9106..9876
                     /gene="sigG"
                     /locus_tag="P5549_02055"
     CDS             9106..9876
                     /gene="sigG"
                     /locus_tag="P5549_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HRW11889.1"
                     /translation="MINKVEICGVNTARLPVLKNEEMRKLFAAMQAGGSDAREKLIQG
                     NLRLVLSVIQRFSNRGEYVDDLFQVGCIGLIKAIDNFDLAQNVRFSTYAVPMIIGEIR
                     RYLRDNNAIRVSRSLRDIAYKALQVREKMLAENSTEPSISQLAEQLEVPREEVVFALD
                     AIQEPVSLFEPIYNDGGDPILVMDQISDDVNADDHWLQSITINEALKRLSEREKHIMA
                     LRFFYGRTQMEVADEIGISQAQVSRLEKAALKQLKKYV"
     gene            9893..10033
                     /locus_tag="P5549_02060"
     CDS             9893..10033
                     /locus_tag="P5549_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11890.1"
                     /translation="MNKNLLVMVLLVIILSMAVPLTNYLQQSHYAFTCNNVISVRPGL
                     DY"
     gene            10113..11471
                     /locus_tag="P5549_02065"
     CDS             10113..11471
                     /locus_tag="P5549_02065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRW11891.1"
                     /translation="MSRQNSIKLEWAGKDRPFMQEEPVVLLPEIQHVYPFPLFTDSNL
                     LASPAPTDTPMMNQYYQADNLHVLQALSQAENKPSIQLIYIDPPYMSQIHYRSLKEDR
                     QQPAFADVWQPESYLAMLYPRLQLMKEVLADNGSIFVHVDWHVSHYVHLLLDEIFGPD
                     NFINEIVWCYSGGGNSRRHLLRKHDLIFWYAKSSAYTYHPQHRPYTKGTLARGLTAVK
                     GDKYKLAAEGALLQDWWTDINKILSPTAHENLKYPTQKPRQLLKRIITMASNPGDRVA
                     DFFAGSGTTGEVCNQLGRSWIMCDNSSLALQTTLSRLVVTNSPPFAIHACALSAEDTC
                     GGEKDAAAAALRLQDVALQPQGTDQVWLSMGIDHFMPQDLPESNVLKDFSETIEFWEI
                     DPEYDGKIFRSRYQILRSHHQRSGDLPLNVLLSQPLRQQYRIAVKVHDIFARQYLRVL
                     AF"
     gene            11617..12705
                     /gene="dprA"
                     /locus_tag="P5549_02070"
     CDS             11617..12705
                     /gene="dprA"
                     /locus_tag="P5549_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRW11892.1"
                     /translation="METNELASLFCLHSLDRIGTRTLWKIKSYFGSFQACFEASRQNL
                     QASFLSEEIISRILTARASIDRQQMLQDFMQTGGRISCVEDEDYPSWLGTIHDPPYLL
                     YYQGELACLNQLCIGIVGSRRASSYGRTQSRRFGRELAATGITVVSGMARGIDTEAHR
                     GALEAGGKTAAVLGSGLDVIYPAENKALYQEIVAHGVVLTEFSPHTHPEPGNFPVRNR
                     IISGLSRGILVVEAQQKSGALITVDFALEQGRDVFALPGPINIKTSVGSNHLIKQGAC
                     MVTEINDILSEYGLVQDNTAMMQQGDIFALNHAEKVVLNLLELDSQHFDELVGQSKLT
                     IGELSTVLLQMELKGIVKALPGNYYVKI"
     gene            12753..14819
                     /gene="topA"
                     /locus_tag="P5549_02075"
     CDS             12753..14819
                     /gene="topA"
                     /locus_tag="P5549_02075"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRW11893.1"
                     /translation="MSETTLVIVESAAKAKTISKFLGKNYKVKASVGHIRDLPKSKLG
                     IDVEKDFEPQYITIRGKGELLREIKTEAAKADRVLLATDPDREGEAIAWHLQTAMKLA
                     PGSKCRIEFNEITKDAIKKAVKSARPIDIDRVDAQQTRRILDRLVGYNLSPLLWSKVR
                     KGLSAGRVQSVAVKLICDREKEIDAFVSEEYWSIETILRAGKKDTFTAKLSTYKNKKI
                     EIGSGGQKDKIVNDLQKARISVVNIDTKEKRKRPYPPFITSTLQQEASKRLNFFSSRT
                     MRVAQELYEGLALGKEGTVGLITYLRTDSTRIAAGAQQEALQYIAEHFGQAYVPATPN
                     QYKSRKSAQDAHEAIRPTSIYRSPEAIKAYLSRDQYKLYKLIWERYAASQMSQAVYDT
                     ITVEMAAGDYGLRAYSSQLKFSGYKKVYNDYDEEEAKNPIPALQEKQELKLQDILPEQ
                     HFTQPPPRYTEASLVKLLEEKNIGRPSTYAPIIETILKRRYVVRENKQFSPTELGFVV
                     VELLNEHFGDILDVEFTAKLEENLDAIEEGEAGWKQVIREFYVPFKEDLDKAQDLIEK
                     IEIKDEEAGRDCPQCGRPLFIKYGRFGKFIACSGFPECRYTESINEEIGVDCPFCGSP
                     IIALKSKKGRKYFGCKAYPECSFRSWNKPTGEKCPHCGDAMVEKTSRGKEAQTVCQNP
                     QCKAQQ"
     gene            14831..16165
                     /gene="trmFO"
                     /locus_tag="P5549_02080"
     CDS             14831..16165
                     /gene="trmFO"
                     /locus_tag="P5549_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRW11894.1"
                     /translation="MVIKPVNVIGGGLAGAEAAWQLAERGIPVKLWEMRPQIKTAVHR
                     SENLAELVCSNSLKSNLADTAQGLLKNEMEQIGSLLLACARATQVPAGSALAVDRERF
                     SALVTRRLQAHSRIEIIREEMTSIPGEGVSIIATGPLTSEKMFQEIAALGGDDNLYFY
                     DAIAPSVTFNSLNQNKIFKASRYGKGSDDYYNCPLNREEYEKFWQELTAADIVEGHSI
                     DQKDFFSGCMPVELIARRGLDTLRFGPMRPVGLTDPRTNQRAWAILQLRQENHEGTVF
                     GMVGFQTRLRWKEQDRIFRMIPGLEAAEFIRYGVMHRNTYINSPRLLLPTLQNKNHPA
                     LFFAGQITGVEGYMESAATGILAGLNAARFVAGQELLVLSPLTMIGALVHFITSAAAE
                     HFQPMNANFGILPALEQKIKDKKSRYQHYVERSQKEMCKISELFLEHVVKEF"
     gene            16208..17119
                     /gene="xerC"
                     /locus_tag="P5549_02085"
     CDS             16208..17119
                     /gene="xerC"
                     /locus_tag="P5549_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRW11895.1"
                     /translation="MLMKYEQEFLTHMRVEKSASNLTLLSYRTDLDQFFDFLSRENEQ
                     LLEDLSTDMINHKTVRKYLAQMQAAGLQRATIARKLAALRSFVKYMCREDILPGNPIA
                     NVSTPKQDRRLPRFLYPAEINLLLDAPDTASYLGRRDKAILETMYASGVRVSELVAIN
                     LADIDWGGETILIRGKGNKERLAPLGRQARIALRTYLDNSRSLFIQAGKPVPEAVFLN
                     RFGGRLSGRSIRNIIDKYVDEVALTQKVSPHTLRHSFATHLLNGGADLRSVQELLGHV
                     KLSTTQIYTHLTKENIKTIYDNSHPRR"
     gene            17124..17657
                     /gene="hslV"
                     /locus_tag="P5549_02090"
     CDS             17124..17657
                     /gene="hslV"
                     /locus_tag="P5549_02090"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRW11896.1"
                     /translation="MFEATTIIAVKKGTQMAIAGDGQVTMGQNTIMKNNAVKIRRLYD
                     GKILAGFAGSVADAFTLFARFEEKLRETGGNLTRSVIELAKDWRSDRVLRKLEALLIV
                     ADLHTMFIVSGNGEVIEPEEGIAAIGSGGNYALAAARALTRFTDLQAAQVARESLLIA
                     SQICVFTNDHISVEMLE"
     gene            17690..19093
                     /gene="hslU"
                     /locus_tag="P5549_02095"
     CDS             17690..19093
                     /gene="hslU"
                     /locus_tag="P5549_02095"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRW11897.1"
                     /translation="MNRQLTPKEIVEELDKHIIGQKEAKKAVAIALRNRYRRKLLPEA
                     LSEEIYPKNIIMIGPTGVGKTEIARRLAKLVKAPFVKVEASKFTEVGYVGRDVDSMIR
                     DLLETAIRMVKSEKMEEVEDKGRVLANERIVDAILPMPRKKSKGFKNPLEMLLGQNQE
                     NSFHDDEFEQRSKEIEQKRAIISQKLDRMELEQEIIEIEVEEKSPSFMEIFSGSGVEE
                     MGINMQEALGGLMPKKTKKRKVTVEEARKILTQEEAQRLIDMDGVSREAIQRVEENGI
                     VFLDEIDKIASQENNHGPDVSRGGVQRDILPIVEGSTVMTKHGPVKTDHILFIAAGAF
                     HVCKPSDLIPELQGRFPIRVELESLQKDDLQRILIEPDNSLLKQYKALLSTEGLELGF
                     AADAVEYIAQTAYEVNARTENIGARRLHTIMEKVLEDLLFESAYMQGQKITIDQDYVQ
                     QRLKKIVDNDDLSRYIL"
     gene            19107..19889
                     /gene="codY"
                     /locus_tag="P5549_02100"
     CDS             19107..19889
                     /gene="codY"
                     /locus_tag="P5549_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HRW11898.1"
                     /translation="MKSILEKSRAINRILKKISSSAVDFYEMAEVLAENMECSAIIVD
                     RKGNIMGYALVGEEHCPELEKLIKDKKPFPPIINQEILNITETKSNTILGDNRTCMFN
                     VENPECSCATRLWTICPNFGGASRIGTLILTRNNGQIFTDEDIVLAEYGTIVVANEIM
                     RMRTERMEEDARRKAAVQIALATLSYSEREAIENIMVELDGSEGLLVASRIADKVGIT
                     RSVIVSALRKFESAGVIEARSLGMKGTHIKVLNDYIFDELKK"
     gene            20082..20444
                     /locus_tag="P5549_02105"
     CDS             20082..20444
                     /locus_tag="P5549_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW11899.1"
                     /translation="MGKRVLIVDDAAFMRMMIKDILDKNGYEIAGEAVDGMGALQKYK
                     ELSPDLVTMDITMPEMDGVDTVKAIIEFDPDAKIIMVSAIGQQAIVIDAIQAGAKDFI
                     VKPFMPERVIEAINKVLR"
     gene            20502..21572
                     /locus_tag="P5549_02110"
     CDS             20502..21572
                     /locus_tag="P5549_02110"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HRW11900.1"
                     /translation="MEFISVLVVDDSAFMRKVIKDIINSDPLMQVVGTAKNGQEALLK
                     VELLCPDVITMDIEMPEMDGLTALEILMETNPVPVVMLSNLTQLGAKATIKALQLGAV
                     DFIPKPSGAISLDIATISEEIINKVKTAASAHVIPSTRTAAHLITERVDKTSFKGNSG
                     ENQLDKLVLIGASTGGPKALQQIISALPGDIDAALLLVQHMPAGFTKSLAERLNASSS
                     ISVKEAEDGEPILTGHAYVAPGGYHMRVHQQKLPLGDRMVIKLQQDEPRAGHRPSINE
                     MFLSVAAQFAGEVTGVILTGMGNDGTAALPALRNKGAWIIAEDQSSCVIYGMPKSAIE
                     SGYVNEVLPLSEISAAIVKLLQ"
     gene            21611..23638
                     /locus_tag="P5549_02115"
     CDS             21611..23638
                     /locus_tag="P5549_02115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HRW11901.1"
                     /translation="MELDMNKYLDVFLEESREHIRTLNSRLLQLEKNPQETSGLNEIF
                     RSAHTLKGMSSTMGFIALADLTHHMENLLAELKEGKMQVTSRAIDILFACIDRLQIMI
                     DDIAGGKKPHEDNADLIHALETIRSAQAETAAGTTQHAEAVVLTVDDAPDTRAHGMIY
                     NDYDITILKEALSRGFNVQYLNIGVAPNSMMKAVRVFMVFKALEESGEVIKTNPSAQE
                     LDEGKFDDQFELIYICEGDLTKALARINQISEVKLLGNHAVDPSELTVTEPVIKAGPQ
                     SSAGAAEVKKNEPDMVAPVRHTVRVDIEKLDLLMNLVGELVMHKGRLEQIGVLYKMEE
                     LNNAIEQIDRITSDLQSVVMKVRMVPIEQVFNRFPRMVRDLAKELDKEVDFYMEGKET
                     ELDRTVIDEIGDPLLHLLRNAIDHGLELPQQRLSLQKKRQGQLYLRARHEGNHVFIEV
                     EDDGAGINPDKVRERAIESGLLGIREAEQLSDEEIIKLVFESGFSTAEVVTDISGRGV
                     GMDVVKSKIEALSGEIIVNSKPGYGSLFKIKLPLTLAIIQAMMVTVQSETYAIPLTSV
                     DETTMISARDIKVIQGQEFITLRGSILPLLRLNRLVELPETDQTTDEIYVVVVHKGSK
                     QVGLVVDTLIGQQEIVIKSLGKILDGIPGIAGAIIARDGSVCLILDVATLF"
     gene            23650..24123
                     /locus_tag="P5549_02120"
     CDS             23650..24123
                     /locus_tag="P5549_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HRW11902.1"
                     /translation="MSLSKVPLEKNQSPGETMQVVVFFLAGAEYAVEIKQVEEINRLL
                     NITRVPHAPSYIEGVINLRGNIIPVINLHKKFNLSGQEFDPQTRIIVLKINDLNAAII
                     VDRVSEVIYLEVDHIVADTQFFSPINAEVIEGMVKREDRLIIVLDLLKLLNKEIN"
     gene            24129..24761
                     /locus_tag="P5549_02125"
     CDS             24129..24761
                     /locus_tag="P5549_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRW11903.1"
                     /translation="MSVLHEGWDKISKMQLDVLREIANIGAGNAATALATMLKSRIEI
                     SVPHALIMPFADVPGFMGGAEAHSVGIYFHVSGPAQASILLVMPVEKAAQLLEMLLGT
                     SPASMSGPFSNLEYSALMELGNILSSSYLNALSSFTRISFKPSPPALAVDMVGSILNT
                     ILSQYGEVSEMVLVMETGFKKDKVDVVGNIFLLPEPDSLNTLFNALGVVV"
     gene            24761..25246
                     /locus_tag="P5549_02130"
     CDS             24761..25246
                     /locus_tag="P5549_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRW11904.1"
                     /translation="MGELIQVGMAECKLARPPDKLMTAGLGSCVGICLLDPITKIASM
                     CHIMLPYCHHDKPAVNPGKYADTAILAAVGAMHRSGADTDHLIAKIAGGAQMFRFSGN
                     SDIFKIGQRNVLSVEENLEKNKIQLLGKDIGGNVGRTIVFDPATGNLAIRTIHEGDKI
                     I"
     gene            25426..26124
                     /gene="rpsB"
                     /locus_tag="P5549_02135"
     CDS             25426..26124
                     /gene="rpsB"
                     /locus_tag="P5549_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRW11905.1"
                     /translation="MAVITMKQLLEAGVHFGHQTRRWNPKMSPYIYMERNGIYIIDLQ
                     QTVKKFDEAYAFVKEVVADGKGILFVGTKKQAQETIKDEAARCNMYFVNQRWLGGMLT
                     NYKTIRKRVMRLKELERMETDGTFDVLTKKEVAHLRNEAERLERFLGGIKDMDKLPGA
                     VFVVDPRKERIAVAEARKLNIPVVAIVDTNCDPDEIDYIIPGNDDAIRAVKLISAKIA
                     DAVLEGRQGEQVTA"
     gene            26214..26867
                     /gene="tsf"
                     /locus_tag="P5549_02140"
     CDS             26214..26867
                     /gene="tsf"
                     /locus_tag="P5549_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRW11906.1"
                     /translation="MMEITAVMVKELRERTGAGMMDCKKALVAMEGDIEKAIDELRTK
                     GLAKAAKKAGRVASEGVVISYIHGGGRIGVLVEVNCETDFVAKTDEFKQLAYDVAMQI
                     AATNPVCISREQVSEELLQREREVLRAQAIEEGKPEKIIEKMVEGRIEKYYKENCLLE
                     QGYIKDPDKTLQEMIHESIAKIGENIAVRRFARFELGEGIEKEETDFAAEVMSQIKS"
     gene            26910..27626
                     /gene="pyrH"
                     /locus_tag="P5549_02145"
     CDS             26910..27626
                     /gene="pyrH"
                     /locus_tag="P5549_02145"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRW11907.1"
                     /translation="MHEPKYKRIILKLSGEALAGDNGYGIEHNTITSIARQIVELTEI
                     GVEVAIVVGGGNIWRGVAGSARGMDRATADYMGMLATVINALALQDALESEGVVTRVM
                     SAIGMQAVCEPYIRRRAIRHLEKGRVVIFAAGTGNPYFSTDTTAALRSAEIEADAILM
                     AKKVDGVYDADPKTNPNAQKYTRLDYIDVLNQGLAVMDSTAASLCMDNKIPIIVFNLT
                     VEGNILRAVMGEDIGTYVGR"
     gene            27632..28189
                     /gene="frr"
                     /locus_tag="P5549_02150"
     CDS             27632..28189
                     /gene="frr"
                     /locus_tag="P5549_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRW11908.1"
                     /translation="MINDILQDAEDRMKKTIEHMLGDFASMRAGRANPAMLDRIMVSY
                     YGEPTPINQMANINVPEARLLVIQPWDKSSIADIEKAIMKSDLGITPNNDGNVIRLAV
                     PQLTEERRKDVVKTVKKRAEEAKVAIRNIRREGNDLIKSSEKDKLISEDESKKGLDSI
                     QKVTDKYIKDIDAALQVKEKDIMEV"
     gene            28515..29255
                     /locus_tag="P5549_02155"
     CDS             28515..29255
                     /locus_tag="P5549_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRW11909.1"
                     /translation="MARKTSQQTLDPRKMPRHVAIIMDGNGRWAKKRFMPRTMGHRAG
                     MSSLKKVVRACDDMGIAILTVFAFSTENWKRPGDEVGYLMQLLTEYLHKELDELHQNK
                     VRIRVLGDYQILPLECRVEIQRALQMTRDNTGLVFNIALNYGARQEIMEAAKKLAEKM
                     AVGEIKAEDLSEEIFSGLLYTSGMPDPDLLIRSSGEMRISNFLLWQIAYTELVVVDTL
                     WPDFSEKDLVQAVWEYQQRDRKFGGLSK"
     gene            29274..30059
                     /locus_tag="P5549_02160"
     CDS             29274..30059
                     /locus_tag="P5549_02160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRW11910.1"
                     /translation="MLKTRIITAVIGVPLLLAILYLGGLAWSALFVLLAVVALYEYLA
                     MMQQNGQSPQYLPAFMLLFLLLFQTQTGPCFTPLLMAVIMLIVLEGVFFYPQFTLADT
                     AQSFLGAFYIGFLLHFALLIQDLPKPFLTILLIFLVTWGSDIGGYVFGRLWGKRKMTP
                     LLSPNKTWAGAMGGVLMATLAAVGFEYFVPLAGFSLGKMIFLAAIASVAAQLGDLFES
                     AMKRSFGVKDSGWIIPGHGGVLDRFDSFMLVLPVFYYFLSLAC"
     gene            30123..31244
                     /gene="ytvI"
                     /locus_tag="P5549_02165"
     CDS             30123..31244
                     /gene="ytvI"
                     /locus_tag="P5549_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HRW11911.1"
                     /translation="MDQEIKKNLLLLLKTSSIVIAMFGLYLLLVYLFPVMGRILGYIP
                     NLFLPFIFAILIAILVEPVVNFFETKLRFKRVLAVLTSLLLVIGGFVYLVSMLVTAII
                     KQLTTIYRATQSNADSMIADMIGTISEVRLIFLRLDLPLQIQNTLQSSLQQAIEWAQH
                     LISAIINSLVTFVTLLPGLMIFLMIATVATFFIIKDRALIRSFVLHIIPPGMRDSGQD
                     VLGKLIKAFVGFVKAYSILISITFILTLVAMKILNVQYALTLALIIALADILPVLGPG
                     AIYVPWIIWEFISHHLAMGIALLAAYVIISAVRQFLEPKIVGDNIGLHPLVTLMSLYV
                     GLKLGGVVGMIMGPVCVVIFIAIYRTGVLDRYDWRDTGE"
     gene            31237..32400
                     /locus_tag="P5549_02170"
     CDS             31237..32400
                     /locus_tag="P5549_02170"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRW11912.1"
                     /translation="MNNTVVNLIVLGSTGSIGRQALEVVDLHPERFRVLGLAAYDELD
                     LLEMQVRKYQPRMIALGNASLYNECRARIGDRAETLTGVEGLCELAAMEGADTVLAAL
                     SGAVGIKPTLAAIKKSRRIALANKETLVAAGDIVMQAARDYHAEIIPVDSEHSAVFQC
                     LQGERPYVQNIWLTASGGPFRDHTREQLAEVTVEMALSHPNWNMGPKISIDSATMMNK
                     GLEVIEAHHLFAIAYDNIKVLVQKESVIHSMVEFVDGSFIAHLGVADMRIPIQYALTY
                     PERKISPASHLDFTALQAIHFAAPDQQRFPALALAIAAGRRGGTLPAVMNAANEVAVG
                     AFIQKQIKFDQIAVIVERVMQNHVVSDQPGLDQILNADRWARDYAGQCVLRRK"
     gene            32402..33433
                     /gene="rseP"
                     /locus_tag="P5549_02175"
     CDS             32402..33433
                     /gene="rseP"
                     /locus_tag="P5549_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRW11913.1"
                     /translation="MTVVLALIIIAVLILVHEAGHFVAARRIGIPVYEFSLGFGSKLF
                     STKKDGVEYSLRLIPLGGYVRMAGEEPGDADNPNGFNHRTPLEKMIVSFAGPFMNFVL
                     AILIFIYTFAVIGIAQPVDDAILGEVIKDKPAYEAGLQSGDRILEVDRQKVSSWQDFV
                     GIIQDEPAGQPVLLTVQREGKNLALSVTPTVNESNGKSMLGVYSQIEYERQGIITSIK
                     MGFIQTYQMTVLLLQGLGMLISGGASTSDLAGPVGIASMVGEAARGGIVYLLSFSAFL
                     SINLGILNLLPIPALDGSRFVFAIVEAIRRKPMDPEREGFIHWLGFLFLMGLIVLATY
                     NDILRLIKG"
     gene            33439..34497
                     /gene="ispG"
                     /locus_tag="P5549_02180"
                     /gene_synonym="gcpE"
     CDS             33439..34497
                     /gene="ispG"
                     /locus_tag="P5549_02180"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRW11914.1"
                     /translation="MMRRKSRVVNIGSCRIGGDNPVVVQSMCSTDTRNVEATVAQIRA
                     LEAAGCQITRVAVVDSEAAAAIRRIKADIAIPLVADIHFDYRLALQSLAAGADALRIN
                     PGNIGDRKRVQAVVSACKEKNIPIRIGVNSGSLDKQMLQRYGAVCAEAMVESALENVK
                     LLEDMDFREIKISLKASSVRLTLEAYRLMAARVDYPLHIGITEAGTKDRALIKSALGL
                     GMLLHEGIGDTIRVSLTGDPVDEVWAAYEILRNLGLSQRGVELISCPTCGRCEIDMIP
                     LAEAVDQAIRNLEQPLKVAVMGCVVNGPGEAREADVGIAGGRGFGLLFRQGEIIRKVP
                     AAEMLSALLQEIDKLSKK"
     gene            34513..36219
                     /locus_tag="P5549_02185"
     CDS             34513..36219
                     /locus_tag="P5549_02185"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRW11915.1"
                     /translation="MKASQLFFPTLRELPSEAEIVSHQLLLRAGYMRKTGAGIYSYLP
                     LAVRVLKKIEQIVREEMNRADGQEILMPIIQPQELWEKSGRWSVYGDEMFRLQDRHKR
                     GFALGPTHEEIITTLVDADVQSYRDLPLMLYQIQNKYRDEIRPRFGLMRGREFIMKDL
                     YSFDLDEEGLDVSYKKMYAAYCAIFHRLGLEFRVVEADSGAIGGNESHEFVVLADSGE
                     SVIVYCDRCDYAANVEKAECVLPEPAKEAPPLQEMEKIHTPGVKTIQELVGFLGHPAH
                     EQAKTLMYMADGELIAVILRGDRELNEIKLQHALQASELFMADEMTVREQCGAGFGSL
                     GPVGLPLKVYADPEITRLYNFSAGANQDDYHLVNINLGRDFVPEEIIDVRNAEAGDIC
                     PHCGGTLQSTRGIEAGHIFKLGTKYSDAMEASYRDQNGQDQPIVMGCYGIGISRTMAA
                     AVEQKNDQDGIVWPLPIAPYHLIIVPVNSKKEDQMEAAERLYQEFSARGVEVLLDDRD
                     ERAGVKFKDADLIGIPVRITIGPKSLQENKVEVKKRMDKENLLLTLDEVVDKVLAMLN
                     AE"
     gene            36249..36914
                     /locus_tag="P5549_02190"
     CDS             36249..36914
                     /locus_tag="P5549_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRW11916.1"
                     /translation="MEALEIVFRLALACILGGIVGLERESLNRPAGFRTYTVVCVGSA
                     LAMIVSIDMYTQFHHYVTADPGRIAAQVISGIGFLGAGTIMKEGATIRGLTTAAGLWV
                     VACIGLAAGAGMYLAAIVATASILFVLIYFARFEHRFTGMRLYKGIVILVDDQPGQVG
                     IIGSILGDMNVLIKEINLDREETENQLEIELLLQLPPEISIMEVIERLSNIQGLHHID
                     RLN"
     gene            36990..40646
                     /locus_tag="P5549_02195"
     CDS             36990..40646
                     /locus_tag="P5549_02195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HRW11917.1"
                     /translation="MAISFYSFVMDKLEPELACLWESTLIQKVEVSGTQRFWRFYLRL
                     PEPLPENAIQATEAALRSRLVFLDQLEIVPALHDYAGSIKDILQRRKADWTAGCFPGY
                     AQHQEYQTIDWQIADDRIDLLADQLTCYQKIIDADVCSRLAAWFWQEYRLGILVRVVC
                     RQLQNPNNPPVTLIGQERPEIISLAPEENKKKRNFGQNKKAVSQVIKAPAMAINELQE
                     GMKAAVTEGEVWFKEVNVLKDGRLVISYYLTDHTDTMIIKTFVDATKDDKIEIGDYIK
                     VKGAIRFDPFIKEAVLYMDDCNKLERRQRVDQSDKKRIELHAHTKMSSLDGLCEVKAL
                     INKAAAWGHPAIAITDHGCIQAFPLAFAAVKALQSAGKELKIIYGVEAYLIEEDKKER
                     PYHIILLAKNQTGLRNLYQLISLSYLDYFYRKPKMRRADLELYREGLLLGSACEAGEL
                     YQALLNHASEEQLDAIARFYDYLEIQPLSNNQFLLKEGRVQSEEELMDLNRRIIALGE
                     KHNKLVVATGDVHFIEPEDQIYRTIIQAGQGYEDADAQAPLYFKNTEEMLQDFAWLGA
                     ETARQVVIDSPNQIAAMIETLQPVPSGFYPPQIDSAEQEITDLTWENARRRYGQPLPE
                     IVEARLRRELGSITTHGFSVLYLIAHKLVKKSNEDGYMVGSRGSVGSSLVAFLTGITE
                     VNALPPHYLCTNCYFNEFFCQGEVGSGADLADKTCPHCGAVLNKDGFDIPFETFLGFD
                     GDKTPDIDLNFSGDYQSRAHQYVEELFGSENVFRAGTISTVAEKTAFGFVKKYIEDKQ
                     LQLKNSEITRLAGGITGVRRTTGQHPGGLIVVPRDHSILEFTPLQHPADKKESGITTT
                     HFEYHALDEQLIKLDILGHDDPTVIKELEDLTGIQARSISLSEPETMKLFSGVEPLKV
                     KPEDIDSSVGTYGIPEFGTRFVRQMLEATRPTSFSELVRISGLSHGTDVWTHNAQDLI
                     EARTAALSEVICTRDDIMTYLIQKGLDKKQSFKIMEKVRKGKKLNQEEADTMLAQGVP
                     RWYVDSCNKIEYMFPKAHAVAYVTMAYRIAWFKVYYPLAFYASFFGIRAEDFEAATIL
                     SGYEPVRQRIKEIEKMGYNASQKEKKLVTILELAMEMYARGYGFLPVDIYESDARKFM
                     VKGNKLLLPFAALPNIGEAAAQGVVGARADGPFISVEDFQVRTHLNKSAMELLRQENC
                     FADLPEKSQMSLFA"
     gene            40818..41270
                     /gene="rimP"
                     /locus_tag="P5549_02200"
     CDS             40818..41270
                     /gene="rimP"
                     /locus_tag="P5549_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRW11918.1"
                     /translation="MAKATAQQVEAMITPILAAIGIELVEVEYRKEHGEQMLRIYIDR
                     EEGVDLSTCTQATRAVQDTIDEANIAYDHLEMSSPGLNRLLNKELDFIRFQGERVKVR
                     TRQALAGQKNFSGRLGQVDQAHLNLEIDGQIQSIPRDIISIVRLDPEI"
     gene            41283..42347
                     /gene="nusA"
                     /locus_tag="P5549_02205"
     CDS             41283..42347
                     /gene="nusA"
                     /locus_tag="P5549_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRW11919.1"
                     /translation="MSNELMQALIDLEKERGIPKAALIEAIRMALNTAYKKNFGTSQN
                     VNVEMNEITGAVQVYSQKMVVAEVTDKSTEILLEEALQIDPDAQLDNLINIEITPSNF
                     GRIAAQTAKQVVVQKLREAERSMIFEEFADREGEIVTGTIQRIESKIVVVNLGRTEGI
                     MLPSDQISNERYEVGHRIKGYIYEVKNSTKGPSIFISRSHPAFLKRLFELEVPEIYDG
                     LVEIKSIAREAGARSKISVHSLEEGIDSVGACVGPRGIRVQNIVNELNGEKIDIIKYS
                     KEPEKFIANALSPSKVIAVEINEAEKKARVIVPDYQLSLAIGKEGQNARLAAKLTAWK
                     IDIKSESQAEEELYEDGESS"
     gene            42331..42597
                     /locus_tag="P5549_02210"
     CDS             42331..42597
                     /locus_tag="P5549_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRW11920.1"
                     /translation="MAKVRKIPQRMCVGCREMKNKRDLIRVVRTPEGSIEVDLTGKKS
                     GRGAYLCNNPECLAQAVKGKRLQKALEHEISNDILDIIKTQLKG"
     gene            42623..42967
                     /locus_tag="P5549_02215"
     CDS             42623..42967
                     /locus_tag="P5549_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRW11921.1"
                     /translation="MKKVYNLLGIAQRAGKLSSGTMAAKQSLTRHRAVLLILSDDIAA
                     NSREDLLNCSQRQRIPAIALGSKFDLGTALGKAYRVALTINDIGIAKAIMQELAESGE
                     EIKTMGVVEWPK"
     gene            42955..>44093
                     /locus_tag="P5549_02220"
     CDS             42955..>44093
                     /locus_tag="P5549_02220"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HRW11922.1"
                     /translation="MAKIRVHELAKELNIPSKEMVVTLQNLGLDVKNHMSTMEDSQAS
                     WVRKKLTEPPVREHAQKKTEAAPRQNVAPAQKTPEPPPRPKPKPPATDSSATVTRGKE
                     PIKRTDDGRKQSPLDAARPQAGKEPRPAPSTGQGGMRDRSGRPTPPAQTGHTPAPGIK
                     PQGQFENRPAPGIKPQGQFENRPAPGARPQGQFDRRPAPGARNDNRPRPQNAPPRTEQ
                     RPQSGTAPQRRPEGQQPGKPGPVRTDAGGRPIQRPQPGQNQTNNRPAAQEKSNRVAPA
                     NKPGGDNKGRAAAPGKGFTQNKGGPTVRRDYSRPQRKGKHKRKKEDNVMLTPESITIG
                     ASVQVRELAEAMGKTAAEVVKKLMELGTMATINQEIDYDTAEIVA"
BASE COUNT        12072 a         9934 c        11603 g        10484 t
ORIGIN      
        1 attcaggagg gagttgattt gctggtcata tcctcagccg ttccccccga gaatattgaa
       61 gtgaaaactg ctatacaaaa gggcatacct gtccttaaaa gaggggagat gctggcccgg
      121 ttggccaatg ccagcaaagg gatcgcagtt gccggagcgc atgggaaaac cacaaccact
      181 tccatggtct ataccgtatt ggagcaatgt ggtgtgcaac ccacctacat cgccggcgga
      241 gagttgcagg acagtgcact taacgcccat ttggggcagg gagaatattt tgtggttgag
      301 gccgatgaaa gcgatgcctc atttttagcc ctgcagccat atatttcgat cgtaaccaat
      361 gtggaaaatg atcatcttga tttttatcaa tcatttgacc gcatcaaagc agcctttcag
      421 aaatttctcc agcaaaccga taatgatggc tttgtattgt tgaacggagc agatccctgc
      481 ttgcaggaga tcagctgctc agctgcggga cgcgttcttt tatatggaga agacagcagt
      541 tttgattact atctgcagga ttggcaggta caggggatgg gttccgtctt tgaggcctac
      601 cgggcaggac agcatttagg caaaataaca ctgtccgtac cgggacgaca taatgcctgg
      661 aatgcgctgg ccgcattggc cgtcgccatt gaactgggtc atccgtttgc tgccgtcgcg
      721 gccagtctca aggacttcca tggtgccaaa cgccgttttc aaatcatggg acatatcggt
      781 aacagtgtgg ttgttgatga ttatgcccat catcccacag aaattcaagc cacgctcaag
      841 gcagcccgtg atttggattt tgctcggctg ctggtcattt ttcaaccgca ccgttatacc
      901 cgtacccatc ttctcgccga ccagtttgcc gtttcatttt ccgcggccga tctggtcatt
      961 atcaccgata tttattccgc gggagaaaaa cccattgagg gcgtagacag tcatctgatc
     1021 tgtcggcttg cagcccaggc cggcgccaac atcaaatatg caagtacctt ggaagaagca
     1081 gaaagcgcaa tccgtcagga agtaaaagat catgatctga ttatgaccat gggggcaggg
     1141 gatatttgga aactgggagt caagttggtg gatgaaaagt aattaacccg tgtctggacc
     1201 ggaaatcagc ggaggtgttg atttggaaga gcacttattg gtcagggggg gcaaggctct
     1261 caaaggccag gtcagtataa gcggttcaaa gaatgccatt ctgccgatta tggcagccag
     1321 tctgctgtca acaggtgagg ttatactcaa tgaagtacct gacctggaag atatcaatgt
     1381 gatggcgcag gcgctcaaaa ttctgggagc tgaaatcaaa cgtgaaggaa aaaacctgat
     1441 tatcattgga agcggagtca acaactgtga actcccggaa agtgtttccc ggcgcatgcg
     1501 agcttccaat ctggtcatgg gcgcttcgtt gggtcgtttg cgggaggccc gggtggcctt
     1561 cccgggaggc tgtgcgattg gctcccggcc catggatttg catatcaaag gatttaaagg
     1621 actgggttat gaaatcagcg aaggcagcgg gtatctgctt ggcaaagcaa aacatgccca
     1681 gggcaaggaa atcatgttgg atgtgcccag tgtaggtgcg actgaaaaca tcatgatggc
     1741 agctgtttta actcctggat atacggtgat tagaaatgct gcacgtgaac cggaaatcgt
     1801 tgatttgcaa aactttatga atcgaatggg agccagagtc aaaggggccg gattggatac
     1861 catcagagtc gaaggcgttg aaaaactagg tggggtggag cacacggtta ttccagaccg
     1921 tattgaagct ggaactttta tggtggctgc tgccatcagt cgtggtgatg tatggatcga
     1981 aaatgtagtg gtggaacatt tgcagccggt tatttccaaa ctgaccgagg taggggtgga
     2041 aatactgcct tgggctgggg gactgcgatt aattggaaac aaagactatc ggcctacaga
     2101 tattaaaacc atgccttatc caggctatcc gaccgacatg caagcccaga tgatgagcct
     2161 tttggcgact gttccgggca ccagcgtgat ggttgaaacg atctttgaga accgctttat
     2221 gcatgttcag gagttgcgtc gcatgggggc agatatcaag attgaaggcc gcgtggcaat
     2281 tattaaaggc gggcaatacg aaggggccac ggtggagagt accgatctgc gtgccggtgc
     2341 tgcccttatt atagctggtc ttgccgctca tggtgaaacc aaaataggga aactggaaca
     2401 tattgatcgg ggttatgaaa aaatccacga aaaattccag aaccttggcg cagaaattac
     2461 aagaatatga cgacatggaa cagatgaata atcccgtttt tccggcctgc cctgcaagtc
     2521 ttttaaaatt gcttgctggg gtgggtttta ttttgcaaga aacgagaaaa ttttgtgtac
     2581 attatattaa gagataatat ccaaaccgat tacaatttaa cttgcaaccg gagtcaatca
     2641 cgattatcct taatttgtgt gttatattgt aaacatgaat tttgatataa ttgcgctgaa
     2701 tgaattggaa aatagcggag taaatcgata tggcgaagaa agctggaaaa atgtcattgc
     2761 tgctcctttt ggttatggta tgtatttatc ttttcttgca cagttcagtt tttaatatca
     2821 gcaaagttta tgtcagcgga caaaaaaaag taaccagtga tgaggtaaaa gcacttgccg
     2881 gagtaactcc cggtgaaaac ctgtttaaaa ttgatggtga tctgattgaa aaatcggtcg
     2941 aaatccatcc cgtagtaaaa aacgcacaaa tgatcagaca tttgccgcat agcgtagaga
     3001 ttaaaatcga agagcgtgtc agttgggcgg tcgttcctta tgaagattta ttcctgctga
     3061 ttgatgatga aggccattgt ctcgataaac taaacaaact tcctgatggg aacaccccca
     3121 ttattaccat ggaacaaatg ccggccaggg tgaatctggg acaagcggtc aacccggaag
     3181 tcataaaaat gatcaaaact gtctggaaat cgattgacgc tgaaggaaga gcccaaatat
     3241 cccaattcca ctatgccaac acacagaagt cattgcttat ttataccctg gaaggaacag
     3301 aagtgcgtta cggcaacctg gagagggtgg aggaaaaagc cgccaacttt accgaagcat
     3361 taaaaatcga agccagtttg caaaaagagg gcaaagacca gctggaatac gtagatttaa
     3421 gattttccgg ccgtcccgtg gtgaaaacca aaacatagaa aggtgataca aatgcgcagc
     3481 aaaacaggac aagtatcgat catgctggtt tgtctggtat tagggatcat gctggcagtt
     3541 caatttagaa atacgcaaaa tactgatgtt aacctccgcg gagccagaac agacgaagtc
     3601 tatgtacggc tcatggaagt gactgaggaa agagatgcat tggccaagga agtattatcg
     3661 ttgcgggaaa aaatggtcaa tgtcagcaac aacaacgatg ccaaccggga actgcaggaa
     3721 gaaatgcaga aagccaatat ggcagcaggg ttgttgccgg ttcatggaca aggcatcgtg
     3781 gtaacgttaa atgacagtgc tagaactgta cagataggcg aggatcccaa tggcttgctg
     3841 gtgcatgatg tggacatact taatgttgta agtgaactca aggcttccgg ggcagaagcc
     3901 atctctgtca acgaggagcg catcattgcc atgtccgagg tacgctgtgc cggaaccaca
     3961 atccttgtca ataccaacaa gattgcaccg ccctttatca ttaaagccat cggtgatccg
     4021 caaatgctgg aaagcggact gaccatcaaa ggcggcgttg cccaaaaatt aaaagattac
     4081 ggtctgcagg tacagataca aaaaatggat gatgtcgaag tccctgctta taccggggtt
     4141 ataaaaacca aattcagtca actgaaaaag taaagattat cagtgccgct gccttgacac
     4201 aaaatgcgtt tcttatttct gcggtggcac ctttttccct ttaaaaatca atgcaaaggc
     4261 ggcatgaatt atgtggctgt tgaccctgtt ttgtctgctc atcggtttgt ttatcggttt
     4321 tcaaatcccc atattattac cggcagtttt tgtaaaatac atgtcaatcg gggttttggc
     4381 agcactcgat tcagtgtttg gcgggattcg tgcctatatg gaagattctt ttgacagcac
     4441 tattttcatc agcggtttta tcaccaatac tctgttggcc ggtggactgg cttatctggg
     4501 ggatcgtctt ggccttgaac tttacttggc agcggtggtg gtttttgggg taagactttt
     4561 ccaaaatcta ggagttattc gtcgatatct attgaaaaaa tactgataaa aattcttcgt
     4621 aaaaagaggg attgccaata cgtttgtggt atttatgaca catgtaaaca aaggggtgtt
     4681 caagtattaa caaacgcaac gtggttgtag ggctggatgt tggtacgagt tcggtaaaag
     4741 cagcgctggc ggaggtcgta tctcccggtg cggtcaatat cctgggtatc atccatcagc
     4801 cctccgcagg cctccgcaaa ggaaatatag tggatattga agcgactgcc cagtcgattg
     4861 acagttgtct caatgaactg gaaagactga ccggtgtgga aattcagagt gctttctgcg
     4921 gcttttccgg catgagtgtc agtgcagtca gtaatcatgc cgtcattgcc gttgccaacc
     4981 ccaatgatga aatcaccgag gaagacaaac gccgggtttt gcaattggcg caaaacgttg
     5041 cgcttccccc cgataaaatg attgttcagc tgatcgaaca gcagtatatt gtagatggct
     5101 atgacggggt caaggatccg gtaggcatga tgggcagccg tctggaagtg gaagtaatga
     5161 ttatcattgc cgccgcggca gcgctgcaga atctgcaaag atgcgctgac cgcatcaatc
     5221 tgcatacaga aagatttgtt tacagtccaa ttctgatgga ggaagcggtt ttgatgccgg
     5281 ctgaaaagga aatgggcgtc gcgctggtcg atctgggcgg agggactact gaaatcagtg
     5341 tctttgaaca gggagttctt ttatcaacca gcgttttacc gattggcggt gagtatatca
     5401 cccgcgacct ggccatagtc cttagaacgt cgattgaaga agcagcccga atcaaagaag
     5461 aaagcggcgt cgctgatatc tcgatggcca gctcggaagt catgatcaat gttcgcaata
     5521 tacaaggcag cgaggtaaaa caggtatccc aacatgtgat tgctgatatt gtagctgcga
     5581 gggtagcaga gatagccact atggttcatg ccgaactaat gcaaaccacc gatcttgaca
     5641 agctgcccgg cggattggtt ttgtgcgggg gtggtgccca gttaaaaggg attgccgagg
     5701 ttatggaagc gtatttgaac ataccggtac gcattggcag accggacaat ataagaggat
     5761 tgcagaggga atacaatctt cccggaaatg ccgtcgtact tggctgtata aattatgcat
     5821 cccggcgcgc aagtttcagt ttaatcgatc ataatcacaa atttggtaaa gcttttggca
     5881 ggataacata ttggctcaag gatttatttg gttaataaca gaggaggtta cgcaatgcca
     5941 ttggattttg atgtggaaat gcagcagttc gcggatatca aggttatagg agtaggagga
     6001 ggcggcaaca acgccatcaa cagaatgatc gaagcaggac tcaaaggagt cgagtttata
     6061 tcggtcaata ctgattctca ggcattgttc ctgtccaagg cggaaaagaa gattcaagtc
     6121 ggagaaaagc ttaccaaagg cctgggtgcc ggtgctgatc ctgagattgg catgaaagct
     6181 gcccaagaaa gcgaagatga tatcctgcga gcccttcagg gtgctgacat ggtgtttgta
     6241 accgcgggta tgggtggagg taccggcaca ggcggtgcgc cgatcattgc ccgtctggcc
     6301 aaacaggtcg gagccttgac agttggtgtg gtcaccaagc ccttttcttt tgaaggccgc
     6361 aaacgcaatc agcaggctga aaccggtatt caggaattaa aaaatgaggt cgacagtctg
     6421 atcaccatac ccaacgacag attgctgcaa gtagtggata aaaatacggc atttaacgat
     6481 gccttccgca ttgctgatga catattgcgc caaggtgtac agggaatatc tgatctgatt
     6541 gctgttcctg gggtaattaa ctgcgatttt gcagatgtca aaacagttat gaaaaataca
     6601 ggttcggcat tgatgggaat cggtcgtgca tccggagaaa accgttcagc ggaagccgcc
     6661 cgtatggcag tttccagtcc cttgctggaa acgtcaatcg aaggagcacg cggcgtcttg
     6721 tttaatatca gcggcggaag caatctgact ttgtttgaga tcaatgaggc ggctgagatc
     6781 atccatcaat ctgcagatgt ggaagccaat attatctttg gggccaacat caatgaagag
     6841 ctggcagatg aagtgatggt aacggttatt gccaccggat ttgatgccaa aaagccgttg
     6901 ctgagtgaac cctccctgcg ttccgccaga ccggtggaaa gcgcccagag cattctggac
     6961 aaacatgatc ttgatattcc tccgtttatg cggcgcagcc ggtaggtttt aagcaaactt
     7021 cacctacctt tcccttcgat tttgtcgatg tatcctaaac cgggcaggaa atggcccggt
     7081 ttatttcttt ttctgcagga agaaggagag gggctgtacc acaaaacaac aatttcttga
     7141 tcccccattt actataattt ggaatacata cttgagcaaa ttgatcatat tcctgttgaa
     7201 agatccattt tgcctggggc aaaccaattg caacagggag caatgatccc cgccggcaag
     7261 gagactgtat gtctgacaat aaagtatatg cagatctgac atttctgctc aatgtgagca
     7321 tggatttttt gttgctctgg ttgaccgcca gattatgtca tttatccata gcgtaccgtc
     7381 gcttgttgct ggcagcttgc ctgggcggtt tttatggcgt cggccagctc atatcagcag
     7441 gcagtattct ctataccttg cccgccaaac tggtggtatc ctgcctgctc ttgctggtcg
     7501 cttgtccatc gaaggcctgg gcgcaatttg gcaaagcctt catggtattt tacggtttga
     7561 gctttatcac tgccggagcc agcctggctg cagcgtctct ggtgccaacc ggagaagacg
     7621 cttcctggtc aatgtattat ctggtggcag gggtgagcgc actcattagc attgcaatat
     7681 ttgccgaacg cttttttcgg caaacgttgc tgccggtgtt gttgaaatac caggtgcgac
     7741 tctgtttcga taacaagtgc tgccggggac agggatttct ggataccggc aatggactgc
     7801 gcgatccact gactcatcgt ccaattgtgg tagcagaatt cgccctggta caatcctgtt
     7861 tgccggaaga gatttgttgt gccatggaag atgatctgaa tgaagagcaa cgtttggaag
     7921 cgttaagcca cagccagtgg ggaaaccggg taaggttgat cccgttttct tctttgggga
     7981 aaaaaaatgg aatgctgatt ggactgcgct gtgacgagtt ggggataaga gtgggacagc
     8041 gggaaatgct gcataaaaac ctggtagtgg ccatttatcc atataaactg agtgccaatg
     8101 gtgattatca actgctgata ccgtctgaag ttgtgcaaca agcatgaggg gggagcgagc
     8161 atgaacctga gggaaaaatg ggaaaccctg aaactacgtt tattaaaatg gtacatcaaa
     8221 agaaaactgc ctggatacat tcattacata gggtctgccg aagtgttgcc acctcccttg
     8281 aaggaaagtg aagaaagcaa tctgatttcc cgtctggaca gcggtgatgg cgcagtcaaa
     8341 tcaaccttga ttgagcacaa tttgcgcctg gtcgtgtata ttgccaaaaa gtttgagaat
     8401 accggcatta acatcgaaga tttgatatcg ataggtacca ttggcctgat caaagcggtg
     8461 aatacatttg aacccagtaa aaaaatcaaa ttggcaacct atgcctcccg ttgcattgaa
     8521 aacgagatcc tcatgtattt gcgccgcaat ttaaaaaacc gggccgaagt atctcttgat
     8581 gaaccgctca atgtggattg ggacggaaat gaattgttgc tctcggacgt gctgggcaca
     8641 gaagccgata tgatttataa aaccatcgaa ggagaagtgg aaaaatcctt gctgcagcag
     8701 gctatgcaca agcttaatcc ccgggaaaaa atcatcatcc agctgcgttt cgggcttaac
     8761 aatggctatg agaaaaccca gaaagaagta gcagatatcc ttggcatatc ccaatcctat
     8821 atttcaagat tggaaaaaag aattttaaaa cgactgcagc gcgagatacg caagattgaa
     8881 taatcatgtc tttccattac tacatgttcc gctgcctgct gggatgctca acaggcggcg
     8941 gtttttttat gcgtgccggc agaaaaaccg ggcacaacgt tcgagggccg gacagagaat
     9001 aattaatcat gtaaatgtaa ataatgaata aaaatctgta cgcatcaatg ccttgcttac
     9061 aggcatgctt ttgaaaaagc gggatgagca ggaaggaggc actccttgat caacaaggtg
     9121 gaaatttgcg gcgtaaacac tgcccggttg ccagttttga aaaatgaaga gatgcgcaaa
     9181 ctttttgctg ccatgcaagc cggcgggagt gatgcccggg aaaaattaat ccagggcaat
     9241 ttgcggctgg ttttaagcgt cattcaacgt ttcagcaatc gcggcgaata tgtggatgat
     9301 ctatttcagg tcggttgtat tggcctcatc aaggcaatcg ataattttga tctggcccag
     9361 aatgttcgct tttcgaccta tgcggtgcct atgatcatcg gcgagatccg ccgctatctg
     9421 cgtgacaaca atgccatccg ggtttcgcgc tcgctgcgcg atatcgctta taaagcattg
     9481 caggtcagag aaaagatgtt ggcggaaaac agcactgaac catcgatatc acagctggcc
     9541 gaacagcttg aggtgcccag ggaagaagta gtttttgcct tggatgcgat acaggaaccg
     9601 gtctctctat ttgaacccat ttataatgac gggggagacc cgattctggt gatggatcag
     9661 atcagtgacg acgtaaatgc agacgatcac tggctgcaat ccataaccat caacgaagcc
     9721 cttaaacgct tgagcgagcg agaaaagcat atcatggcac tgcgtttctt ctatggacgc
     9781 acccagatgg aagtcgctga tgagatcggg atctcccagg cgcaggtatc acggttggaa
     9841 aaagctgcgc tcaagcaatt gaaaaaatat gtttaacatc aggaggccca agatgaataa
     9901 aaacctgctg gtcatggtat tgctggtcat tattctcagc atggccgtgc ctttgaccaa
     9961 ttatcttcag cagagtcact atgctttcac ctgcaacaat gtgattagtg tccgcccggg
    10021 acttgattat taaacattgt gctaatttgt tgctgattcc tgccgtggtt ttagggtaga
    10081 atgaaaagaa agaaaagcaa aggcaggaca caatgagcag gcaaaattcg atcaaattgg
    10141 aatgggcagg gaaagacagg ccttttatgc aagaagaacc tgttgttttg ttgccggaaa
    10201 tacagcatgt ttatcctttt cctttgttta ctgacagcaa ccttttggcc agtcctgccc
    10261 ctacagatac tccaatgatg aaccaatatt accaggctga caatctgcat gtcctgcaag
    10321 ccttgtccca ggctgagaac aagccatcga tccaactcat ctacattgat ccgccctaca
    10381 tgagccagat ccactatcgt tctctcaaag aagacagaca acagccggct tttgctgatg
    10441 tctggcagcc ggaaagttat cttgccatgt tatacccccg tttgcagctt atgaaagaag
    10501 tgttggctga caacggcagt atttttgtac atgtcgactg gcatgtgagc cattatgtac
    10561 atttgctgtt ggatgaaatt tttggcccgg acaattttat caatgaaatc gtttggtgtt
    10621 acagcggcgg ggggaattcc cgacgtcatc tgctgcgcaa acacgacctc atcttttggt
    10681 atgcgaagag ttctgcctat acgtatcatc cccagcatcg tccctatacc aaaggtactt
    10741 tggcaagagg gcttaccgcg gtaaaaggag ataaatacaa attggcagcg gagggtgccc
    10801 ttctgcagga ctggtggacc gatatcaaca aaatcttaag ccccactgcc catgagaatc
    10861 tcaagtatcc cacccaaaaa ccccgccagc tgttgaaacg aatcatcacc atggcatcga
    10921 acccggggga tcgggtagca gatttctttg ccggctccgg gacaacagga gaggtctgca
    10981 atcaattggg cagatcatgg atcatgtgtg acaatagttc tttggccctg caaaccacgc
    11041 tcagccgttt ggttgtgacc aactcaccac cttttgcgat ccatgcctgt gccctgtccg
    11101 cagaagatac ttgcggggga gaaaaagatg ctgccgctgc ggcgttgcgt ctgcaagacg
    11161 ttgccctgca accccaaggc acggatcaag tatggttgag tatgggcatc gatcatttta
    11221 tgccccagga tttgccggag agcaacgtgt taaaagattt ttccgaaacg atagaatttt
    11281 gggagatcga ccccgaatac gatggcaaga tcttccgttc ccgttatcaa atcctgcgtt
    11341 cccatcacca aagaagcgga gacctgccgt taaatgttct cttatcccaa ccactccgac
    11401 aacaataccg gattgcagta aaagtacacg atatttttgc ccgtcaatac ctgcgggtcc
    11461 tggcgtttta gacaggttgc cggggtgcaa tccatgctgg gaaaaacaag aagcgcttgc
    11521 attgagaagg tatttatgtt atcaatggct ttagaactta tttccagttt cgcccggaca
    11581 tattttcggt gagctgagtg agctgaggtg agctagatgg agacaaacga gttagccagt
    11641 ctcttttgtt tgcatagcct ggatcgcatc gggacccgca ccttatggaa gatcaaaagt
    11701 tatttcggca gctttcaggc ctgttttgaa gcttcaaggc agaatctgca ggcctctttt
    11761 ttaagtgaag aaattatcag ccggattctg actgcccggg catccatcga cagacaacaa
    11821 atgctgcagg attttatgca aaccgggggt cggatcagtt gtgtagaaga tgaggattac
    11881 ccaagctggt tggggacaat acatgatccg ccctatttgc tctattacca gggggagttg
    11941 gcatgtttaa accagctttg tataggcatc gtggggtcac gtcgggccag cagttacggc
    12001 agaacccaat cccggcgttt tgggcgggaa ttggcagcaa caggcattac ggtggtaagc
    12061 ggcatggccc gcggcattga tacggaagcc caccggggag cattggaagc aggcggaaaa
    12121 actgctgctg tactgggcag tggtctggac gtcatctatc ctgcggaaaa caaagcactt
    12181 tatcaggaaa ttgttgccca cggggtagtc ctcacagaat tctcccccca tacccacccc
    12241 gaaccaggca attttcccgt gagaaatcgc atcatttccg gactgagcag aggcatcctg
    12301 gttgtggaag cccaacagaa aagcggagcc ttgatcacgg ttgattttgc ccttgaacag
    12361 gggcgggatg tttttgccct gcccggcccc atcaacatca agaccagtgt cgggagcaac
    12421 cacctgatca aacagggggc ttgcatggta acggaaatca atgatatatt atccgaatac
    12481 gggcttgttc aagacaatac ggcgatgatg caacagggag atatatttgc cctgaaccac
    12541 gccgagaaag tggtcctgaa cctgctcgag ttggattcgc agcactttga tgaactggtt
    12601 ggccaaagca aactgaccat tggcgaactc agcaccgtgc tgttgcagat ggaattaaaa
    12661 ggaatagtta aagccctccc cggtaattac tatgtaaaga tataaatccc acgacagagc
    12721 gaatagaccc aatactaaga tgaggtgata ttttgtcaga aacaacactg gtaatcgttg
    12781 aatcggcagc caaagccaag accatcagca aattcctggg caagaattat aaagtgaaag
    12841 catctgtcgg ccatatccgt gatttgccca agagcaagct tggcatagat gtagaaaagg
    12901 attttgagcc tcaatatata acgattcgcg gcaagggaga gctgctgcgc gaaatcaaga
    12961 ccgaggccgc caaggcggat cgggtattgc tggccactga cccggaccgc gaaggggaag
    13021 ccattgcctg gcatctgcag accgccatga agttggctcc aggttcaaaa tgccgtattg
    13081 aattcaacga aatcaccaaa gacgcgatca aaaaagccgt caagagcgca agacccatcg
    13141 acattgatcg ggttgacgcc cagcaaaccc gacgcatcct cgatcgcctg gtgggctata
    13201 atttaagtcc gctgctgtgg agcaaggtgc gcaagggatt gtcggccgga cgcgtacagt
    13261 ctgtggcggt aaaactgatc tgcgatcgtg aaaaagagat cgatgctttc gtctctgaag
    13321 agtattggag catagaaacc attttgcgtg cggggaaaaa agacaccttt accgccaaac
    13381 tcagtactta taagaataaa aaaattgaaa ttggttctgg cggacaaaaa gacaagattg
    13441 tcaacgattt gcagaaagcc cggatatccg tggtcaatat cgataccaag gaaaaacgca
    13501 agcgtcccta tccgcctttt atcaccagta cgctgcaaca ggaagcttcc aaacgcctga
    13561 attttttttc cagccgcacc atgcgggtcg cccaggaatt gtatgagggc ctggctttag
    13621 gcaaggaagg caccgtaggt ttgatcacct atttgagaac cgattctacc cgaatagctg
    13681 ccggtgctca gcaggaagca ctgcagtata tcgccgaaca ttttggacag gcgtatgtac
    13741 ccgcaacccc caatcaatat aaaagcagaa aatccgccca ggatgcccac gaagcgattc
    13801 ggccgacttc gatttatcga agccctgaag ccatcaaggc ctacctgagc agggatcagt
    13861 acaaactgta caaactgatt tgggaacggt atgcagccag ccaaatgagc caggctgttt
    13921 acgacaccat cacggtggaa atggcagcgg gggactatgg actgcgcgcc tattcatccc
    13981 agttgaaatt ttcgggatat aaaaaagtat ataacgatta tgatgaagaa gaagccaaaa
    14041 atcccatacc ggcgctgcaa gaaaaacagg aactgaaact gcaggatatt ctgccggaac
    14101 agcattttac ccagccgccc ccccgttata ccgaagccag tctggttaaa ctgctggaag
    14161 agaagaacat tggccggccc agtacatatg cgcccatcat tgaaaccatt cttaaacggc
    14221 gttacgtggt acgcgaaaac aagcagttta gtccaacgga attgggattt gttgtagtag
    14281 agctgctcaa tgaacatttt ggggatattc ttgacgtgga atttacggcc aaactggaag
    14341 agaacctgga tgccattgag gaaggcgaag ccggttggaa gcaagtgatc cgggaattct
    14401 atgtgccctt taaggaggat ctggacaaag cccaggactt gattgaaaaa atcgaaatca
    14461 aagatgagga agccggccgg gattgtcccc aatgtggccg ccccttgttc attaaatacg
    14521 ggcgcttcgg caagtttatc gcttgttcgg gatttccgga atgcagatac acagagtcaa
    14581 ttaatgaaga aatcggcgtt gactgcccgt tttgcggcag tcccatcatt gccctcaaat
    14641 ccaaaaaagg cagaaaatat tttggctgca aggcctatcc cgaatgcagt ttccgatcct
    14701 ggaacaagcc caccggagaa aaatgccctc attgcggcga tgccatggtg gaaaagacca
    14761 gccgcggcaa ggaagcccaa acagtgtgcc aaaatccgca atgcaaagcg cagcaatagc
    14821 agggggcgat atggtgataa aacctgtaaa tgtaatcggc ggagggctgg caggcgcaga
    14881 agcagcctgg cagttggccg agcggggaat ccctgtaaag ctttgggaaa tgagaccgca
    14941 gattaaaacc gctgtacatc gctctgagaa tctggccgag ctggtatgca gcaattcgct
    15001 gaaatcaaat ctggctgaca ccgcacaggg tctgctgaaa aatgaaatgg agcaaatagg
    15061 ctccctgttg ctggcctgtg cacgggcgac ccaggtgcct gccggctcag ccctggcggt
    15121 cgaccgggaa cggttttctg ccttggtcac ccgtcgcctg caagcgcatt cccggataga
    15181 aatcatcaga gaagaaatga cctcgattcc tggtgagggg gtcagtatta ttgctaccgg
    15241 tccgctcact tcagaaaaga tgtttcagga aattgctgcg ctgggcggcg atgacaacct
    15301 ttacttctat gatgccattg cgcccagcgt gacgtttaac agtcttaatc aaaataaaat
    15361 attcaaagcc tcccgctatg gtaagggcag tgatgattat tacaactgtc ccctcaaccg
    15421 ggaggagtat gaaaaattct ggcaagagct gacggctgcg gatatcgttg aaggacacag
    15481 tatcgaccaa aaggattttt tttccggttg catgccggtt gaactcattg cccgccgggg
    15541 attggatacg ctgcgttttg gtcccatgcg cccggtggga ctgacagatc cccgtaccaa
    15601 tcaacgtgcc tgggccattt tgcaattgcg ccaggaaaac catgaaggca cggttttcgg
    15661 gatggtcgga ttccagacca gactgcggtg gaaagaacaa gaccggattt tccgtatgat
    15721 ccccggcctg gaagcagctg aattcattcg atatggtgtc atgcaccgca atacctacat
    15781 caacagtccc cgtctgctgt tgccgaccct gcagaacaaa aatcatcccg ccctgttctt
    15841 cgccggtcaa atcaccgggg tggaaggcta tatggaatca gccgccaccg gcatcctggc
    15901 cgggttaaat gccgcccgtt tcgttgccgg tcaggaactg ctggtgctta gtcctctgac
    15961 catgatcggc gccctggttc atttcatcac ttctgctgcg gctgaacatt ttcagcccat
    16021 gaatgccaat tttggcatcc tgcctgcgct ggagcaaaag atcaaagaca aaaaaagcag
    16081 atatcaacac tatgttgaaa gatcacaaaa agaaatgtgc aaaatttcag aattgtttct
    16141 ggaacatgtt gtcaaagaat tctaagtatg ctagcatttc attagtaaga aatcggagga
    16201 agaccccatg ctaatgaaat atgagcaaga atttttaacc cacatgcggg tcgagaaaag
    16261 cgcctcgaat ttaacactgc tcagctatag aactgatttg gatcagtttt ttgacttttt
    16321 atccagggaa aatgaacagt tattggaaga tttatcaaca gacatgatta atcataaaac
    16381 ggttcgtaaa tacctggcgc agatgcaagc tgccggattg cagcgggcaa ccatcgccag
    16441 aaaactggct gccctgcgct ccttcgtgaa atatatgtgc agggaagata tcctgccggg
    16501 caaccccatc gccaacgttt ccacccccaa acaggacaga cgcctgccgc gcttcctata
    16561 cccggcagaa atcaatctgc tgcttgatgc acctgatacc gccagctatt tggggcgcag
    16621 ggacaaagcg atattggaaa ccatgtatgc ctccggggtg cgggtgagtg agttggttgc
    16681 catcaatctg gctgacatcg actggggggg agaaaccatt ctgatcaggg gcaaaggcaa
    16741 caaggagaga ttggctccct tggggcgaca agcccggatt gccttgcgga cctatttgga
    16801 taacagccgc agtcttttca tacaggccgg caagccggtt cctgaagcag tatttttaaa
    16861 tcgcttcggc ggacgtttaa gcgggagaag tataagaaat attattgata aatacgttga
    16921 tgaagttgcc ctcacccaga aagtaagtcc ccatacccta aggcattcgt ttgccaccca
    16981 tttgcttaac ggcggggctg atctaaggtc ggtgcaggaa ttgcttgggc atgtgaaatt
    17041 atccacgacc cagatctata cccatctgac caaagagaat attaaaacca tctacgataa
    17101 cagtcatccc aggaggtgaa gcagtgtttg aagccacaac catcattgcc gtcaaaaaag
    17161 gcacgcagat ggcgattgcc ggagacgggc aggtaaccat gggacaaaat accattatga
    17221 aaaacaatgc ggtaaaaatt cgcagactgt acgatggcaa aatactggcc ggttttgccg
    17281 gttcagttgc cgacgccttt accctttttg cccgttttga agaaaaactc agggagaccg
    17341 gcggaaactt gacccgctca gtcatcgagt tagccaagga ttggcgtagt gacagagtct
    17401 tgcggaaatt ggaagcgtta ttgatcgttg ccgatcttca taccatgttt atcgtttccg
    17461 gcaacgggga ggtcatagaa cctgaagaag ggattgcggc cattggctcg ggaggcaatt
    17521 atgccctggc ggcagcccgt gccttgacaa ggtttactga tttgcaagca gcgcaagttg
    17581 ctcgcgaatc gctgttgatc gcttcgcaaa tctgtgtttt caccaatgat catattagtg
    17641 tggaaatgct ggaataaagc cgggttaaaa gcagaaaagg agaaccgttt tgaatagaca
    17701 gttgaccccg aaagaaatcg tggaagaatt ggataagcat atcattggcc agaaggaggc
    17761 caaaaaggcg gttgccatcg ctttgcgcaa ccggtatcgg cgcaagctgc tgccggaagc
    17821 gttaagcgag gaaatatacc ccaaaaacat cattatgatc ggaccaaccg gcgtcggcaa
    17881 gacggaaatt gccagacggt tggccaagct ggtcaaggca ccatttgtca aagtagaagc
    17941 cagcaaattc accgaagttg gctatgttgg ccgcgatgtc gactcaatga ttcgcgacct
    18001 tttggaaact gccataagaa tggtaaaatc agaaaagatg gaggaggtgg aagataaagg
    18061 ccgggtgctt gccaatgaga ggattgttga tgccatcctg cccatgcctc gaaaaaagag
    18121 caagggcttc aagaatcctt tggaaatgct gctggggcag aatcaggaaa acagttttca
    18181 tgatgacgag tttgaacaac gcagcaagga gattgagcaa aaacgggcaa ttatcagcca
    18241 aaaacttgac cgcatggaac tggagcaaga aatcatcgaa attgaagtgg aagaaaaatc
    18301 cccttcgttc atggagatct tctctggctc cggagttgag gaaatgggta ttaatatgca
    18361 ggaagctctg gggggattaa tgcccaaaaa aaccaaaaag cgcaaagtga ccgtggaaga
    18421 ggccaggaaa attctcaccc aggaagaggc gcagcgatta attgacatgg acggagtcag
    18481 tcgtgaagct attcagcgag ttgaagaaaa cggcatcgta tttctggatg agatcgacaa
    18541 gatcgccagt caagaaaaca atcacggtcc ggatgtatcc agaggcggag tacagcgcga
    18601 tatattgcca attgtagaag gttctaccgt aatgaccaag catggaccgg tcaagaccga
    18661 ccatattttg tttattgcgg caggcgcatt tcatgtttgc aaaccatccg accttattcc
    18721 tgaactacag ggaagatttc cgatcagggt cgagctggaa agcttgcaaa aggatgatct
    18781 gcaaagaatt ctaatcgagc cggacaactc tttgttgaaa caatataaag ctttgttgtc
    18841 taccgaaggt ctggaattag gattcgctgc tgacgcggtt gaatacatcg ctcaaaccgc
    18901 ctatgaagta aatgccagaa ccgaaaacat tggggcacgc agattacaca caataatgga
    18961 aaaagtgctg gaagatcttc tctttgaatc tgcctacatg cagggacaaa agataacgat
    19021 tgaccaggac tatgtgcagc aacggctcaa aaagattgtg gataatgatg atctgagcag
    19081 atatatcctg taggaggttg ttttaaatga aaagtatttt ggaaaaatct cgagccatta
    19141 atcggatttt aaaaaaaatc tcatccagtg cggtagactt ttatgaaatg gccgaggttc
    19201 tggctgagaa catggaatgc agtgctataa tcgtcgatcg taaggggaat atcatgggtt
    19261 atgccttggt tggcgaagaa cattgtccgg aactggaaaa actcattaaa gacaaaaaac
    19321 ctttcccacc cattatcaat caggaaatct tgaatattac cgaaaccaaa agcaacacaa
    19381 ttttaggaga caaccgtact tgcatgttta atgtggaaaa cccagaatgt tcatgtgcca
    19441 cccgtctctg gacgatatgt cccaattttg ggggagcaag ccgcatcggc accttgattc
    19501 tgaccagaaa caacggtcag atatttaccg acgaggatat tgttttggcc gaatatggca
    19561 ccattgtcgt agccaatgaa atcatgcgga tgagaacgga acgcatggaa gaggatgccc
    19621 gccgaaaagc ggccgtgcaa attgcccttg ccaccctctc ttattcagag cgcgaagcca
    19681 ttgagaatat tatggttgaa ttggatggtt cggaagggtt gctggttgcc agtcgcatcg
    19741 ccgataaagt aggcattacc cgctctgtga ttgtgagtgc cctgcgcaag tttgaaagtg
    19801 ccggagtgat agaagccaga tccctgggga tgaaaggcac ccacataaag gttttaaacg
    19861 attatatttt tgatgaacta aagaaataat ccagttagcc gggaagataa tctatgaatg
    19921 gactaatcaa cagagattag tctattcttt ttccaatctt tttggaggtg aacgctgcgc
    19981 aggaaaacga ttcatatgat agaataatgg tgaattgttt atgcgatcaa agtcatttta
    20041 ttttgtttca acatgaaaca aacagaaata ggaggttaac gatgggcaaa cgggtattga
    20101 ttgtcgatga tgctgctttt atgaggatga tgataaaaga tattctggat aaaaatggtt
    20161 atgaaattgc gggtgaagcc gttgatggca tgggtgccct gcaaaaatac aaggaattgt
    20221 cccccgatct ggtcacaatg gatataacca tgccggaaat ggatggggtt gatacggtaa
    20281 aagcaatcat cgaatttgat ccagatgcca aaatcattat ggtgagcgcc attgggcagc
    20341 aggccatcgt cattgatgca attcaagccg gagccaaaga ctttatcgtc aaaccgttta
    20401 tgccggaaag agtgattgaa gcaatcaaca aagtattaag gtgataaacc ctggagcgga
    20461 atctttgctg caataacaag gggccagcag ggggtggaga gttggagttc atttcagtat
    20521 tggtcgtaga cgattctgcc tttatgcgta aagtgatcaa agacattatt aacagtgatc
    20581 ccttgatgca ggttgtaggg acggccaaaa acggacaaga agccttgttg aaggttgagc
    20641 tgctttgtcc tgatgtaatc accatggata ttgagatgcc ggaaatggat ggtttaactg
    20701 ccctggagat cctcatggaa accaatcctg tgcccgttgt catgttgagc aatctgactc
    20761 aattgggagc aaaagcaacc attaaagctt tacagttggg agcagttgat tttattccca
    20821 aaccttccgg agcaatatca cttgatatag ccacgatcag tgaagaaatt ataaataaag
    20881 tcaaaacagc tgcgtcagcg catgtaattc cctccaccag aacagcagcc catttaatta
    20941 ccgaacgggt agacaagaca tcctttaagg gaaacagcgg tgaaaaccaa ttggacaaat
    21001 tggtattgat cggtgcctct accggcgggc ccaaagcact ccaacaaatc atatctgccc
    21061 tgcccggcga tattgatgca gccctgttgc ttgttcagca tatgccggca ggctttacca
    21121 aatccctggc tgaaagactg aacgccagca gcagcatcag cgtcaaggaa gcggaagatg
    21181 gggaacccat tttgactggc catgcctatg ttgcgccagg cggctatcat atgcgggttc
    21241 atcaacaaaa actgccgctg ggggatcgga tggtcatcaa gctgcagcag gatgaaccgc
    21301 gggcaggaca tcgaccttcc attaatgaga tgtttctttc cgtggcggcg caatttgcgg
    21361 gagaggtcac aggggtcatt ctgaccggaa tgggaaacga tggtacggca gccttgccgg
    21421 cgctgcggaa caaaggtgcc tggattattg ccgaagacca atcttcctgt gttatatatg
    21481 gcatgcccaa atctgctatc gaatcaggtt atgtcaatga agtcctgccc ttgtcggaaa
    21541 taagcgcagc gatcgtaaaa ctgctgcagt aggaaagaat cacttgtagt gacggcggag
    21601 gtgctcgtgc atggaactgg atatgaacaa gtacttggat gttttcctgg aggagagccg
    21661 cgaacatatc agaactttga acagcaggct tttgcagttg gagaaaaatc ctcaagaaac
    21721 atcaggcctc aacgagatat tccgctctgc ccacacactg aaaggaatgt cttcgaccat
    21781 gggctttatt gcgttggccg atttgacaca tcatatggaa aatttgctgg ctgaactgaa
    21841 ggaaggaaaa atgcaggtta cctcccgagc gatagatatt ttgtttgcct gtatcgatcg
    21901 tctgcaaatc atgattgatg atattgccgg aggaaaaaag ccacatgagg ataacgcaga
    21961 tcttatccat gcgctggaaa ccatccgcag cgctcaggcg gaaactgctg ccggcacaac
    22021 acagcatgcc gaagcagttg tactgactgt tgatgatgcc ccggacacac gcgcgcatgg
    22081 aatgatttat aacgattatg acatcaccat cctgaaagaa gctttaagcc ggggttttaa
    22141 tgttcaatat ctaaacatcg gggttgctcc caacagcatg atgaaagcag tcagagtatt
    22201 tatggtgttc aaagccctgg aggagagcgg agaggtaatc aagacaaatc cttccgccca
    22261 ggaacttgat gaaggcaagt ttgatgacca gtttgaactg atctatattt gcgaaggtga
    22321 tttgaccaag gcgcttgcgc gtatcaacca aatcagcgaa gttaaactgc tgggcaacca
    22381 tgcagttgac ccaagtgaat taacggtaac cgaacctgtc ataaaagctg ggccccagag
    22441 ttcggccggt gctgctgagg taaaaaagaa tgaaccggac atggtcgcgc cggtcagaca
    22501 cacggtcagg gtagatattg aaaaacttga cctgcttatg aatctggtcg gggaattggt
    22561 catgcataaa ggacgtctgg aacagattgg agttctttat aaaatggaag aactcaataa
    22621 tgccattgaa caaattgatc ggattacttc agacctgcag tcggttgtta tgaaagtacg
    22681 catggttccg atcgagcaag tgttcaatcg ctttccccgt atggtcagag acctggccaa
    22741 agaactggac aaggaagttg atttctacat ggagggcaag gaaaccgagt tggatcgtac
    22801 ggtgattgat gaaatcgggg atcccctcct gcatttgctc agaaatgcaa ttgatcacgg
    22861 gcttgaactt ccccaacaac gcttatctct gcaaaaaaaa cggcaggggc agctctattt
    22921 gcgggcacga catgaaggca atcatgtctt catcgaagtg gaagacgatg gcgccggcat
    22981 aaatccggac aaggtaaggg aaagagcaat tgaatccgga ctgctgggaa tacgggaagc
    23041 tgaacaatta agcgatgaag aaatcatcaa gctggttttt gaatccggct ttagtacggc
    23101 agaggtggtc accgacattt ccggccgcgg ggtaggcatg gacgtagtga aaagcaagat
    23161 cgaagccttg agcggagaga ttattgtcaa ctccaaacca ggatacggca gtctcttcaa
    23221 gataaaactc ccgcttacct tggccattat tcaagccatg atggtaactg ttcagagtga
    23281 aacctatgcg atacctttga cttctgtaga tgaaacaacc atgatcagcg cccgggacat
    23341 aaaagtaatc caaggtcagg agtttattac gttaagaggc agcatattgc ctctgctgcg
    23401 tcttaatcgt ctggtcgaac ttcccgaaac cgaccagaca accgatgaaa tatatgtggt
    23461 ggtggttcac aagggcagca aacaagttgg tctggtggta gatacgctga tcgggcaaca
    23521 agagatcgtc atcaagagtt tgggcaagat actggacggt attccgggga ttgccggagc
    23581 gattattgcc cgggatggca gtgtatgtct gatcctggac gttgcaacat tgttttaagg
    23641 ggtgagggaa tgagcctttc caaagtcccg ctggaaaaga atcagtcgcc tggcgagacg
    23701 atgcaagtgg tagtcttttt tttagcgggg gccgaatatg cagtggaaat caaacaggtt
    23761 gaggaaatca atcggctcct caatattacg cgcgttcccc atgccccttc ctatatcgaa
    23821 ggtgtcatta atttgcgcgg caatatcatt ccggtcatta atctgcacaa gaaattcaat
    23881 ttatccggtc aggaatttga tccacaaacc cgtatcattg tactgaaaat aaatgatctc
    23941 aatgccgcca ttattgttga ccgggtttcc gaagtgattt atctggaggt tgatcatatc
    24001 gttgcggata ctcagttttt cagtcccatc aatgcggaag tgattgaggg aatggtaaaa
    24061 agagaagacc gactcatcat tgtactggat ttgcttaaac tgctgaataa ggaaataaac
    24121 tgatcggggt gagcgttttg cacgagggat gggacaaaat atcaaaaatg caactggatg
    24181 tgctccgcga gattgccaac attggagcag gcaatgcggc aaccgctttg gctaccatgc
    24241 tcaaatccag aatcgaaatc agtgtgccgc acgcattaat catgccgttt gccgatgtgc
    24301 ctggctttat ggggggagct gaagctcatt ccgtgggcat ttattttcat gtgagcggac
    24361 cagcccaagc cagtatcctg ctggtcatgc cggttgaaaa agcagcgcag ttgttggaaa
    24421 tgctgttggg taccagtccg gcatcaatga gcggcccgtt ttccaatttg gagtattctg
    24481 ctttaatgga gctgggcaat attctgtctt catcctattt aaatgccttg tcgtcattta
    24541 ccaggatatc ctttaaacct tctccgccgg cgctggccgt tgatatggtc ggctccattt
    24601 taaacaccat tctgagccaa tacggtgaag tgtcggagat ggttctggtg atggagaccg
    24661 gctttaaaaa agataaagtt gatgtggttg gcaatatctt tttactgccg gaaccggact
    24721 cactgaatac gctttttaat gcacttggag tggttgttta atgggagaat tgatccaggt
    24781 aggcatggca gaatgcaaac tggccagacc acctgacaag ctgatgactg ccggcctggg
    24841 ttcgtgtgtt ggcatatgcc tgttggatcc gataaccaaa atcgccagta tgtgtcatat
    24901 catgctgccg tattgccacc acgacaaacc tgcagtcaat cccggcaaat atgctgatac
    24961 ggccatcctt gctgccgttg gcgccatgca caggagcggc gcagacacag atcatttgat
    25021 tgccaagatt gccggaggtg cacagatgtt tcgcttttcc gggaacagcg atatttttaa
    25081 aatcggccaa cgcaacgttt tatcagtaga agagaacctg gaaaaaaaca aaatccaatt
    25141 gctggggaaa gacattggcg gaaatgtggg tcgtaccatt gttttcgatc cggctacggg
    25201 gaacctggca atacgcacca ttcacgaggg agataaaatt atttgatttt acagcctggc
    25261 gctgacaccc tgacaaaaat gactgcaaat cttgccaacg cccgggttga tatgctatag
    25321 tatctgttgg tgattacaca cgttgctgga tcggatgggg ttgtgcttaa aagtccccca
    25381 ctgaaatgaa acaacggagg caaagaaaaa caaggaggca ttggaatggc agttattacc
    25441 atgaaacaat tacttgaagc cggggtccat ttcggacatc aaaccagaag atggaacccc
    25501 aaaatgagtc cctacatcta tatggaaaga aacgggatct atatcatcga tttgcagcag
    25561 accgtcaaga aatttgatga agcgtatgcc ttcgtcaagg aagttgtggc agatggcaaa
    25621 ggaatcctgt ttgttggaac caagaagcag gcccaggaaa ccattaagga tgaagctgct
    25681 cgctgcaata tgtattttgt caaccagcgc tggttgggag gcatgctgac caactacaaa
    25741 accatccgta aaagagtcat gcggctcaaa gaacttgagc gcatggaaac agacggaact
    25801 tttgatgtat tgaccaaaaa agaagtagcc catctgagaa acgaagccga acggctggag
    25861 agattcctgg gcgggatcaa ggacatggat aaactgcccg gtgccgtttt cgtcgtcgat
    25921 ccccgcaagg agagaatcgc tgtagctgaa gccagaaagc tgaatattcc ggtagttgcc
    25981 atcgtcgaca ccaattgcga tccagatgaa attgattaca tcatacctgg aaacgatgat
    26041 gccatcagag ccgttaaatt gatctcagcc aagatcgctg atgcagtatt ggaaggccgc
    26101 cagggagaac aagttaccgc ttaaagatca ggagaagggg gtggggttta cagcaaacac
    26161 accccttttt tgtctgcgca atacagcaag aaaaaacaac aggaggttat ggcataatgg
    26221 aaatcacagc cgtaatggta aaagaactgc gcgaaagaac cggcgcagga atgatggatt
    26281 gcaaaaaagc actggtagcc atggagggcg acattgaaaa agcgattgat gaactccgga
    26341 ccaaagggct ggccaaagct gccaaaaaag caggccgagt tgccagtgaa ggggtagtca
    26401 tatcctatat tcacggcgga ggccggatcg gggtcctggt ggaagtcaac tgtgagactg
    26461 attttgtggc caaaacagat gaattcaaac aattggccta tgatgtggcc atgcagattg
    26521 ctgccaccaa cccggtatgt atctcccgcg aacaggtatc ggaagaattg ctgcaaagag
    26581 aacgggaagt attgagagcc caggcaattg aagaaggcaa accggaaaaa atcattgaaa
    26641 agatggttga aggacgtatc gaaaaatact acaaggaaaa ctgtctgctg gagcagggtt
    26701 atatcaagga tcccgataaa accttgcaag agatgattca cgagagcatc gccaaaatcg
    26761 gtgaaaacat cgctgtaaga agatttgccc gttttgaatt gggtgaaggc atagaaaaag
    26821 aagaaacgga tttcgctgcc gaagtcatgt cccagatcaa gtcctaaccc cgtacaacat
    26881 agccaagaat aatgggggag gtattcatca tgcatgaacc caaatataaa cgcatcatat
    26941 tgaagctaag cggggaagcc ctggccggcg acaatgggta tggaattgaa cacaatacca
    27001 tcacctccat tgcccgtcaa attgtggaac taaccgagat tggcgtggaa gtagccattg
    27061 ttgtcggggg cggcaatatt tggcgcgggg ttgccggcag tgcgcgcggc atggatcgtg
    27121 ccaccgccga ttacatggga atgctggcta cggttatcaa tgccttggct ttgcaggatg
    27181 ccttggaaag tgaaggagtc gtaacccgcg tcatgtcagc cattgggatg caggcggtat
    27241 gcgaaccgta tatccgccga cgggccattc gtcatttgga aaaaggacgt gtggttattt
    27301 ttgctgccgg taccggcaac ccatattttt cgactgatac caccgcggcg ctgcgttctg
    27361 ctgaaattga agccgatgcc attctgatgg ccaaaaaagt tgatggcgta tatgatgctg
    27421 atcccaaaac caatcccaat gcccaaaaat atacccggtt ggattatatt gatgtgctca
    27481 atcagggcct ggcagttatg gattcaaccg ctgcttctct gtgtatggac aataaaattc
    27541 cgattatcgt attcaatctg accgtagaag gaaatatatt aagagcggtg atgggtgaag
    27601 acataggcac atatgtaggg aggtaaaata aatgatcaat gatattttgc aagatgctga
    27661 agatcgaatg aaaaaaacca ttgaacatat gttgggcgat ttcgccagta tgcgtgccgg
    27721 ccgcgccaac cctgccatgc tggatcgaat catggtcagc tactatggtg aaccgactcc
    27781 catcaaccag atggccaata tcaatgtgcc ggaagcccgc ttgctggtca tccagccttg
    27841 ggacaagagc agcatcgcgg atattgaaaa ggccatcatg aaatccgact tgggaatcac
    27901 acccaacaac gacggcaacg tgattcgtct ggcggttcct cagctgaccg aagagagaag
    27961 aaaagatgtt gtcaaaacgg taaaaaaacg cgctgaagaa gccaaagtgg ccattcgcaa
    28021 cattcgccgc gaaggcaatg atctcatcaa gtccagcgag aaagacaaac tcatctccga
    28081 ggatgagagc aagaaaggcc tcgacagtat tcaaaaagtg actgacaaat atatcaaaga
    28141 catcgatgct gccctgcagg tcaaagaaaa ggacatcatg gaagtataac tatttgcgtt
    28201 acccgggggc ctcttgtcgg ctgcaattac gtagcggttg ctggttgttg attgcaacct
    28261 cgatgggatt gcttttgccc ctgctgcgtc cggcaaaaaa ttctcgggcg gacaacctaa
    28321 agcgatcggg gatacagaat gccagtctta aattgctgct ggattatggc tcctgaaatg
    28381 gttataataa taacgaaatg cccccatcgt attgggggtt ttgttatatg accgagcagc
    28441 ctgggctgag tccttgcaca gacaaatcga taccgggaat gagtgcaacc ataacggcag
    28501 agagaaggga taaaatggct cgcaaaacca gtcaacagac cctcgatccc aggaaaatgc
    28561 cccggcacgt agccattatc atggacggga acgggcgctg ggcgaaaaaa agattcatgc
    28621 ctcggaccat gggacaccgc gccgggatga gttcccttaa aaaagtggtg cgcgcctgtg
    28681 atgatatggg tatagccatc ctgacggtat tcgccttctc tactgaaaac tggaaaagac
    28741 cgggcgatga agtgggttat ctcatgcagt tgctcaccga atacctgcac aaggaattgg
    28801 atgaattgca tcaaaacaag gttcgtatcc gggtgctggg ggattaccaa atactgcccc
    28861 tggaatgccg tgtcgaaatc caacgcgcgt tacagatgac tcgagacaat accggacttg
    28921 tctttaatat cgctttgaat tacggagccc gccaggaaat catggaggcc gccaaaaagc
    28981 tggcagaaaa aatggctgtc ggtgaaatca aagcagaaga tctaagcgaa gaaatatttt
    29041 ccggcttatt gtatactagt ggtatgcctg acccggattt gcttattcgc agttcaggag
    29101 agatgagaat cagcaacttt ttgctctggc agatcgctta caccgaattg gttgtggtgg
    29161 ataccctctg gcctgacttt agcgagaagg acctggtgca agcggtgtgg gaataccagc
    29221 agcgggatcg taaattcggc ggtctcagca aataaccgga aggattaaac cgtatgctta
    29281 aaaccaggat cattactgct gttatcggcg ttccactgtt gcttgccatt ttgtatctgg
    29341 gcggactggc ttggagcgcg ctgtttgtcc tcctggcggt cgtggcttta tacgaatatc
    29401 tggccatgat gcagcagaac ggacagagcc cgcagtattt gccggcattt atgctgcttt
    29461 tcctgttgct gtttcagacg cagaccggtc cctgtttcac ccctctgctg atggcagtga
    29521 tcatgctgat cgttttggaa ggtgtattct tctatccaca atttaccttg gcagatacgg
    29581 cgcaaagttt cctgggcgct ttttatattg gctttttatt acactttgcc ctgctgatac
    29641 aggatcttcc caagcctttc ctgaccattc tgctgatctt tctggtgacc tggggatctg
    29701 atattggtgg ttatgttttt ggccggctgt gggggaaaag gaaaatgacc cccttgttaa
    29761 gtcccaataa aacatgggct ggagcaatgg ggggagtgct gatggctacg ctggctgcag
    29821 ttggttttga atactttgtg ccccttgcag gcttcagcct gggcaaaatg atcttcctgg
    29881 cggccatcgc ttcggttgcc gcccaactgg gtgatctttt tgaatcggcc atgaaaagat
    29941 cttttggcgt caaggattcc ggatggatta ttccgggcca cggcggtgtg ctggaccggt
    30001 ttgacagttt tatgctggtc ctgccggttt tttattattt tctttctttg gcctgttaat
    30061 atgtttcgcc agcagactca taaatccaat catacctaat aaaataaaat gaggtgagcg
    30121 atttggatca ggaaatcaag aaaaacctgc tgttattact caaaacaagc agtattgtga
    30181 tcgccatgtt tggtctttat ttgctgctgg tatatctatt tccggttatg ggccggatat
    30241 tgggttatat tcccaatctg ttcctgccct ttatttttgc gattctgatt gcaatcctgg
    30301 tggaaccggt cgttaatttt tttgagacca aattgcgctt taaacgtgtg ttggcggttt
    30361 taaccagttt actgctggtg atcggcggct tcgtttatct agtttccatg ctggttacgg
    30421 ccatcatcaa acagctcacc accatctatc gggcaaccca gtccaatgca gattcaatga
    30481 tcgcagacat gatcggcaca atcagtgagg tgcgtcttat tttcctgcgc ctggatttgc
    30541 cgctgcagat tcagaatacc ttgcagtcaa gtctgcaaca ggcgatcgaa tgggcgcagc
    30601 atttaatcag cgcgatcatt aacagcctgg ttacctttgt caccctgctg ccgggattga
    30661 tgatcttttt aatgattgcc acggtggcga catttttcat tatcaaagac agggccttga
    30721 tcagaagttt cgttctgcac attatccccc ccggtatgcg cgactcgggc caggatgttc
    30781 tcggcaaact catcaaagcc tttgtcggct ttgttaaagc atacagcatc ttgatatcca
    30841 ttacgtttat cttgaccctg gtcgccatga agattctgaa tgtgcaatat gctcttaccc
    30901 tggctttaat cattgcgctc gcagacatcc tgccggtctt gggaccgggt gccatctatg
    30961 ttccctggat tatctgggag tttatttccc atcatttggc gatgggcatt gccttgttgg
    31021 cggcttatgt tatcatttca gccgttcggc agttcctgga gcccaagatc gtgggcgata
    31081 atatcggtct gcatccgctc gttactttga tgtctctcta tgtgggacta aagctgggcg
    31141 gagtcgtggg catgattatg ggaccggttt gcgttgtcat ctttattgcc atttatcgga
    31201 ccggcgtgct ggaccgttat gactggaggg ataccggtga ataatacagt tgtgaatctg
    31261 attgtattgg gatcgacggg ttccatcggc cgacaggcat tggaagtggt tgatctgcac
    31321 ccggaaagat ttcgcgtgct gggactggct gcctatgatg aactggattt gttggaaatg
    31381 caggtcagga aataccagcc gcgtatgatt gctttgggca acgcttcttt atataatgag
    31441 tgtcgggcca gaatcgggga ccgggcagag accctgaccg gtgtggaggg gctgtgcgaa
    31501 ttggctgcga tggagggtgc cgacacggtg ctggcagcct tgagcggtgc ggtaggcatc
    31561 aagcccacct tggcagcgat aaaaaaatca cggcgcatcg ctctggccaa caaggaaacc
    31621 ctggttgcag ccggagatat tgtcatgcag gctgcgcggg actaccatgc tgaaattatt
    31681 cctgtggata gtgaacattc cgcggttttt cagtgcctgc agggagagcg gccttacgtg
    31741 caaaacattt ggctgacggc ttccggggga ccttttcgtg accatacccg cgagcagttg
    31801 gctgaggtta cggtagaaat ggccttaagt catcccaact ggaatatggg ccccaaaata
    31861 agcattgatt cggccaccat gatgaacaaa gggctggaag ttatcgaagc tcatcatctt
    31921 tttgccatcg cctatgataa tatcaaagta ctggttcaga aagaaagtgt gatccactcc
    31981 atggtggagt ttgtggacgg atcctttatc gcccatctgg gagtggccga tatgcgcatc
    32041 cccatccagt atgcgctgac ttatccggaa agaaagatat cgccggcgtc ccatctggat
    32101 tttaccgcgc tgcaggccat tcattttgct gcgcctgacc agcaacgatt tccggcgctg
    32161 gctttggcga tcgctgccgg acgacgcgga ggaacgctgc cggcagtgat gaatgctgcc
    32221 aatgaggttg cggtgggtgc atttattcaa aaacagatca agtttgatca gattgccgtt
    32281 attgtggaaa gagtcatgca gaatcatgtt gtcagcgatc agcccggact ggatcaaatc
    32341 ctgaatgctg atcgatgggc ccgtgattat gccggacagt gcgttttaag gaggaaataa
    32401 attgacagta gttttagccc tgatcatcat tgcggttttg atcttggttc acgaagcagg
    32461 tcattttgta gcagcccgta gaattggaat tccagtctat gaattcagtc tgggatttgg
    32521 cagcaaactt ttttccacca aaaaagacgg ggtggagtat tcgctgcggt tgattccgct
    32581 ggggggctat gttcgcatgg ccggtgaaga acccggtgat gccgacaatc ccaacggctt
    32641 taaccatcgt acgccgttgg aaaaaatgat tgtatccttt gccggaccgt ttatgaactt
    32701 cgtgctggcc atcctcatct ttatttatac gtttgcggtg ataggcatcg cccaaccggt
    32761 cgatgatgcc attctcggcg aagtcatcaa agacaagcca gcctatgagg cgggactgca
    32821 aagcggtgat cggattttgg aggtcgaccg gcagaaggta agcagttggc aagactttgt
    32881 ggggatcatc caggacgaac cggccggtca gcccgttctg ctgacggttc aacgggaagg
    32941 gaaaaacctt gcgctttcgg ttaccccaac ggtcaatgaa agtaacggca aatccatgct
    33001 gggggtatac agccagattg aatatgaacg gcaagggatt attacatcaa ttaaaatggg
    33061 ctttatacag acgtatcaga tgacggtgct gttgttgcag ggcttgggca tgttgatcag
    33121 cggcggggcc tctacttctg atttggccgg gccggtgggg attgccagca tggttggcga
    33181 agcggcccgc ggcgggattg tatatctgct cagtttttct gcgtttttaa gcatcaatct
    33241 ggggatttta aacttgctgc ccatccctgc cctggatggc agtcgttttg tatttgccat
    33301 tgtggaagca atccggcgca aacccatgga tcctgaaagg gaaggtttta ttcattggct
    33361 cggattcctg tttctcatgg gattgattgt actggctacc tacaacgata ttctcagact
    33421 gattaaagga tgatgatgat gatgagacgt aaaagcagag ttgttaacat aggttcctgc
    33481 cgcataggcg gggacaaccc ggtggttgtc cagtcaatgt gcagtactga cacccgcaac
    33541 gtagaagcaa ctgttgccca gatcagggca ttggaagctg ccggatgtca gattacccgc
    33601 gtagccgtag ttgattccga ggctgccgcc gccatccgtc ggatcaaggc ggacattgcc
    33661 attcccttgg tggcagatat tcattttgat taccggctgg ctttgcagag cctggcagcc
    33721 ggggcggatg cactgcgtat caaccccggc aacataggag atcgcaagcg ggttcaggcg
    33781 gtggtcagcg cctgcaagga aaaaaacatc cccatacgca tcggggtcaa cagcgggtcg
    33841 ctggacaagc aaatgctgca gcgttacgga gccgtttgcg ccgaagccat ggtggaaagt
    33901 gccctggaaa acgtaaagct cctggaagat atggattttc gggagataaa aatatccctc
    33961 aaggcctcat cggtcagact taccctggaa gcgtaccggt tgatggccgc ccgggtggac
    34021 tatcctttgc atattggcat tacggaagcc ggaaccaagg atcgggcctt aattaaatcc
    34081 gctttgggtc tggggatgct cttgcatgaa ggcattggtg ataccatccg ggtatccctt
    34141 accggggacc cggtggatga agtttgggca gcgtatgaaa ttctgcgcaa tctgggctta
    34201 agccagcgcg gggtggaact gatttcctgt cctacttgcg ggcgttgtga aattgatatg
    34261 ataccgttgg cggaagctgt cgatcaggcc ataaggaatc tggaacaacc gctgaaggta
    34321 gcggtgatgg gctgcgttgt caacggaccc ggtgaagccc gggaagccga tgtaggcatt
    34381 gccggcgggc gaggctttgg cttgttgttt cgtcagggcg agatcatcag gaaagtacct
    34441 gcggcggaga tgctgtcagc cttgctgcag gagattgata aattgagtaa aaagtaatag
    34501 gaggatggtt ctttgaaagc atcacagttg ttttttccaa ccctgcggga gcttcccagc
    34561 gaagccgaga ttgttagcca tcaactcttg ctcagggcag gctacatgcg caagaccgga
    34621 gccggaatat acagttatct gcccttggcc gtccgcgtat tgaaaaagat cgagcagatt
    34681 gtccgtgaag aaatgaaccg ggcggacgga caggaaatcc tcatgcccat catacagccc
    34741 caggaattat gggaaaagtc aggccgctgg tcggtatacg gtgatgaaat gtttcgtctg
    34801 caggaccggc acaaaagggg ctttgccctg gggcctaccc atgaagagat tatcaccacc
    34861 ctggtcgatg ccgatgtgca atcttaccgt gatctgccgt tgatgctcta ccagattcag
    34921 aacaaatacc gggatgaaat ccggccgcgt ttcggtttga tgcggggacg cgagtttatc
    34981 atgaaagacc tttattcctt cgacctcgat gaagaagggt tggatgtttc ctataaaaaa
    35041 atgtatgccg cctattgtgc catttttcat cgcctgggac tcgagttccg ggtggtagag
    35101 gcggacagcg gggcgatcgg cggcaatgaa agccatgagt ttgtggtgct ggccgacagc
    35161 ggtgaatcgg tgatcgtcta ttgtgaccgg tgtgactatg cggccaatgt agaaaaagcg
    35221 gaatgcgtgc tgccggagcc ggcgaaagaa gcaccgccct tgcaggaaat ggaaaaaatt
    35281 catactccgg gggtcaagac cattcaggaa ctagtcggat ttttaggcca tcctgcccat
    35341 gaacaggcca agacattgat gtatatggcc gatggggagc tcattgcggt gatcctgcgc
    35401 ggagatcggg aattaaatga gatcaaactg cagcatgccc tgcaggcaag tgaactgttt
    35461 atggctgacg agatgacggt gcgtgaacaa tgcggggcag gttttggatc actgggaccg
    35521 gtcgggttgc ctcttaaagt ttatgctgat ccggagatca cccggctgta taattttagt
    35581 gccggtgcca accaggacga ttatcatctg gtcaatatca atctgggccg ggattttgtc
    35641 cctgaagaaa tcattgatgt tcgcaatgct gaggctggtg acatctgtcc gcactgcggc
    35701 gggaccctgc agtcaacccg gggcattgaa gcaggccata tttttaagct gggaaccaaa
    35761 tattccgatg ccatggaagc ctcctatcgt gatcagaacg gacaggatca gcccattgta
    35821 atgggctgct acggcattgg catctcccgc accatggccg cggccgtgga acagaaaaat
    35881 gaccaggacg ggatcgtttg gcctctgccc atcgcgcctt accatttgat catcgttccg
    35941 gtcaacagta aaaaggaaga tcaaatggaa gcggcagaac gtctttatca ggaattttcc
    36001 gcccgcggcg tggaagttct ccttgatgat cgagatgaaa gagccggggt aaagttcaaa
    36061 gatgccgacc tgatcggcat accggttaga attacaattg gtcccaaatc tctgcaggaa
    36121 aacaaggtcg aagtgaagaa acgaatggat aaagagaatc ttctgctgac cttggatgaa
    36181 gttgtcgaca aagtactggc catgttgaac gcagaataaa attgcaggaa agatgaggac
    36241 aggtgatcat ggaagctctg gaaatcgtct ttagactggc gctggcgtgc atactgggcg
    36301 ggattgtagg attggaacgg gaaagtctga accgtccggc cggttttcgc acctataccg
    36361 tggtgtgtgt cgggtctgcc ctggccatga ttgtttcaat cgacatgtat acccaattcc
    36421 atcattatgt tacggctgac cccggacgaa ttgctgccca ggtaatcagc ggcatcggct
    36481 tcctgggtgc cggtacgatc atgaaggaag gcgctaccat ccgcggcttg accacggcgg
    36541 cggggctgtg ggtggttgcc tgcatcggcc tggcagcagg cgccggcatg tatttggcgg
    36601 caattgtggc aaccgcttca atcctttttg tcctcattta ttttgccaga tttgaacatc
    36661 gtttcaccgg catgcgactg tacaaaggca ttgtcatcct ggtggatgac caacccggac
    36721 aagttggaat cattggttcc attctggggg atatgaatgt tcttattaaa gaaatcaacc
    36781 tcgaccggga agaaacggaa aatcaactgg aaatcgaact gttgctgcag ttgcctcccg
    36841 aaattagcat catggaagtc attgagagac tgtcaaacat tcaaggcctg catcatattg
    36901 accgtttgaa ttagatcaag ccatccgatt tcaacctgtc ttggcaggaa taaacacgat
    36961 tttggctgac acacaggagg attattttta tggccatttc tttttacagt tttgtcatgg
    37021 ataaactgga accggaactg gcttgcctat gggaatcaac acttatccaa aaagtggaag
    37081 tgtccggcac gcaacggttt tggaggttct atctgcggct gcccgaacca ctgccggaaa
    37141 acgccattca ggccacagaa gctgccctgc gcagccgatt ggtgtttctt gatcagcttg
    37201 agattgttcc cgctctgcat gattatgccg gcagtataaa agatattttg caacgccgca
    37261 aggccgattg gacagccgga tgttttcccg ggtatgccca gcatcaagag tatcaaacca
    37321 tcgattggca gatcgctgat gaccggatcg atttacttgc tgatcagctg acctgctacc
    37381 aaaagatcat cgatgcagat gtttgttccc ggctggcagc ctggttctgg caggaatatc
    37441 gtctcggtat tctggtgcgg gtcgtctgcc gccagctcca aaatccaaac aatcctccgg
    37501 ttacgctgat cgggcaggaa cggccggaaa tcatttccct ggcacccgag gaaaacaaga
    37561 aaaagcgcaa ctttgggcag aataaaaaag ctgtctccca ggttatcaaa gcaccggcaa
    37621 tggcaattaa tgaattgcag gaagggatga aagcagccgt aaccgaggga gaggtttggt
    37681 ttaaagaagt gaatgtgctg aaggatggac gactggttat cagctattat ttgactgatc
    37741 ataccgatac catgatcatc aagacctttg tcgatgcaac caaagacgac aaaattgaga
    37801 tcggtgatta tatcaaggtc aaaggcgcga tccgcttcga tccgtttatc aaagaagccg
    37861 tgctctatat ggatgactgc aacaaattgg aacgtcggca acgcgttgat caaagtgata
    37921 aaaaacgaat cgaactgcat gcccatacca aaatgagttc cctggatggg ttgtgtgaag
    37981 tcaaggcatt gattaataaa gctgccgcct ggggacatcc cgcgattgcc atcaccgatc
    38041 atggctgcat tcaggccttt ccattggctt ttgctgcagt caaggccctg cagtcagccg
    38101 gaaaagagct gaaaatcata tacggggtcg aagcttatct gatcgaagaa gacaaaaaag
    38161 aacgcccgta tcatattatt ctgttggcca aaaaccagac cgggctgaga aatctctacc
    38221 aactgatcag cctgtcctat ctggactatt tttatcgcaa acccaaaatg cgccgggctg
    38281 atctggagct ataccgggaa ggattgttgc tgggatcagc ctgcgaggcc ggagaattgt
    38341 atcaggcctt gctcaatcac gccagtgagg agcaactcga tgccatcgcc cgcttttatg
    38401 actatctgga aatacagccc ttgagcaaca accagtttct cctcaaggaa ggccgggttc
    38461 agtccgaaga agagttgatg gacttgaacc gtcgcatcat tgctttgggc gaaaagcaca
    38521 acaaactggt tgtggctacc ggggatgttc attttattga gccggaggac cagatctacc
    38581 ggacgattat tcaggccgga caaggctatg aagatgctga tgcccaggcg cccctgtatt
    38641 ttaaaaacac tgaagaaatg ctgcaggatt ttgcctggct gggagcagag accgcccggc
    38701 aggtagtcat tgacagcccc aatcaaattg cagccatgat cgaaacatta caaccggtgc
    38761 cgtcagggtt ttatccaccc cagatcgaca gtgcggaaca ggaaatcacc gatctgacct
    38821 gggaaaatgc ccgccgacgc tacggacagc ccttgccgga aatcgtggag gctcgattgc
    38881 gccgggaact tggctccatc acgacccacg gtttttccgt gctttacctg attgcccaca
    38941 aactggtcaa aaaatccaac gaagatggct atatggtcgg ttcacgtgga tcggtgggct
    39001 cttccctggt agcatttctg accggaatta cggaagtcaa tgcactgccc ccgcattacc
    39061 tgtgtaccaa ctgttacttc aacgaatttt tctgccaggg ggaagtcggt tcgggggcgg
    39121 atctggcaga caaaacctgt ccccattgcg gtgcggtgct caacaaagac ggctttgaca
    39181 tcccctttga aactttcctg ggcttcgacg gagacaagac cccggatatt gatctgaatt
    39241 tctccggtga ctaccagagc cgcgcgcatc aatatgtgga ggaactgttc ggcagcgaaa
    39301 atgttttccg ggccggcacc atctcgaccg ttgcggaaaa gacagccttt ggctttgtta
    39361 aaaaatatat tgaagacaaa caactgcagc ttaaaaattc ggaaatcacc agattggccg
    39421 gcggcatcac cggggtacgc agaaccaccg gacagcatcc cggggggctg atcgttgttc
    39481 cccgcgatca cagtattttg gagttcacac cgctgcagca tcctgccgat aaaaaagaat
    39541 ccggcatcac aactacccat tttgaatatc atgccctcga tgagcagttg atcaagctgg
    39601 atatcctggg gcatgacgat cctacggtaa tcaaggaact cgaagacttg acaggaatac
    39661 aggcccgcag catcagtttg tccgagcccg agaccatgaa actgttttcc ggtgtggaac
    39721 cgctcaaagt gaaaccggaa gacattgatt ccagtgttgg tacctatggc attcccgaat
    39781 tcggtacccg ttttgtccgg caaatgctgg aagccacccg ccccaccagt ttttcggaat
    39841 tggtcagaat cagcggactg tctcacggta ccgatgtttg gacccataat gcccaggatt
    39901 taattgaggc cagaacagct gccctcagcg aggttatctg cacccgggat gatatcatga
    39961 cctatctcat ccagaaggga ctggacaaga aacaatcctt caaaatcatg gagaaggtcc
    40021 gcaaaggtaa aaaactcaat caggaagaag ccgataccat gcttgcacaa ggcgtgcccc
    40081 gctggtatgt ggattcctgc aacaagattg agtatatgtt tcccaaagcc catgcagtcg
    40141 cctatgtgac catggcttac cgtatcgcct ggttcaaggt ctattatccg ctggccttct
    40201 atgcatcctt ttttggcatt cgggcggaag attttgaagc cgccacgatt ttaagcggct
    40261 atgaacccgt ccggcaaaga atcaaggaaa tagaaaaaat gggttacaac gcctcgcaaa
    40321 aagagaaaaa actggtcacc attctggagc tggccatgga aatgtatgcc cgcggctatg
    40381 gatttctgcc ggtcgatata tatgaatccg atgcccgcaa gttcatggtc aaaggcaaca
    40441 agctgctctt gccttttgcg gctttgccca atatcggcga ggctgctgcc cagggagtag
    40501 ttggcgcccg tgctgatgga ccctttatct ccgtggaaga ctttcaggtc cgcacccacc
    40561 ttaataaaag tgccatggaa ctgctgcgcc aggaaaactg ctttgctgat cttccggaga
    40621 aaagtcagat gtctttattt gcctagccgc aaacgttttt atgttgcccg gcaagaaaat
    40681 catccataaa cttgtataat tatattcagt ttgctatact ctattttagt gaacgaatat
    40741 ggacgcaaaa gagtgggtag ccaacccact ctttctattg taatataacc ggagaatgat
    40801 gagcaggggg cgaaatgatg gccaaagcaa cggctcagca ggttgaagca atgatcacgc
    40861 cgattcttgc cgcgattggc attgaattgg tggaagttga ataccgcaaa gaacatggcg
    40921 agcaaatgct gcgtatctat atcgatcggg aggaaggggt ggacctcagc acctgcaccc
    40981 aggctacccg cgccgtgcag gatacaattg atgaggccaa catcgcctac gatcatttgg
    41041 aaatgtcttc ccccggtttg aaccgtttac tgaataaaga gcttgatttt ataagatttc
    41101 agggggaaag agtcaaagtc aggacccggc aggccttggc agggcagaag aatttttcag
    41161 gacgtctcgg ccaggttgac caggcgcact tgaatctcga gatagatgga caaatacaat
    41221 ccattccccg ggacatcatt tcaattgtcc ggctggaccc ggaaatataa ggaggaagaa
    41281 caatgtctaa tgagttgatg caggcattga ttgatttgga aaaagaaaga ggcattccca
    41341 aagcggccct tatcgaggcg atccggatgg cacttaatac ggcctataaa aagaacttcg
    41401 gcacctcgca gaacgtcaac gtcgagatga atgaaatcac cggtgccgta caagtctact
    41461 cgcaaaagat ggtggttgcc gaagtcaccg acaagtcaac ggaaatattg ctggaggaag
    41521 ccttgcagat tgatcccgat gcccaattgg acaatctgat caatattgag atcacaccgt
    41581 ccaattttgg ccgtatcgct gcccagaccg ccaaacaggt ggtggtgcaa aaattgcgcg
    41641 aagccgaacg cagtatgata tttgaagaat ttgctgatcg cgaaggcgaa atcgtcaccg
    41701 gcaccatcca gcgtatcgaa tccaaaatcg tggtggtcaa cctgggacgc actgaaggca
    41761 ttatgttgcc ctccgatcag atcagcaacg aaaggtacga agtcggtcac cggataaagg
    41821 gctatatata tgaagtaaaa aacagcacca aaggtcccag catatttatt tccaggtctc
    41881 acccggcttt tttgaaaagg ctttttgaat tggaagtacc ggaaatctat gacggactgg
    41941 tggaaattaa atccatcgcc cgcgaagcag gagcccgctc caaaatctcc gtgcattcgc
    42001 tggaggaggg gattgattct gtcggtgcct gtgtggggcc gcggggcatt cgggtccaga
    42061 atatcgtcaa cgaattgaat ggggaaaaga tagatatcat caaatacagc aaggaaccgg
    42121 aaaaatttat cgccaacgcg ctcagtccct ccaaagtcat cgcggtggaa attaatgaag
    42181 ccgagaagaa agcccgggtc attgtgccgg attatcagtt gtcactggcc atcggcaagg
    42241 aagggcagaa tgcgcgtctg gctgccaaac tgaccgcctg gaaaattgac atcaagagtg
    42301 aaagccaggc agaggaggaa ctgtacgagg atggcgaaag ttcgtaagat cccgcagcgc
    42361 atgtgcgtgg gatgccggga aatgaaaaac aagcgcgacc tgatccgggt ggtgcgcacg
    42421 cccgaaggca gcatcgaagt cgatctgacc ggcaagaaat ccggtcgcgg agcttatttg
    42481 tgcaataatc cggaatgtct tgcccaggcg gtcaaaggca agcgactgca aaaagcgctg
    42541 gagcatgaga tatccaacga catcctggac ataataaaaa cacagttaaa aggataatcg
    42601 gcactttaag gtgtgtgata gattgaaaaa ggtatataat ttactgggaa ttgcacagcg
    42661 cgccgggaaa ctgtcatcgg gcaccatggc ggccaagcaa agtctgacca gacaccgggc
    42721 cgtcttgtta atcctgagtg acgacatcgc agccaacagc agggaagatt tactgaactg
    42781 cagccaaaga cagcgaatac ccgccattgc tttagggagc aaattcgatt tgggtacagc
    42841 tcttggcaaa gcctatcgag tggcgttaac cataaatgat atcgggatag caaaagcaat
    42901 tatgcaggaa ctggcggaga gcggagaaga gataaaaacg atgggggtgg ttgaatggcc
    42961 aaaataagag tacatgaact ggctaaagaa cttaacatac caagcaaaga gatggtcgtg
    43021 acattacaga atttaggtct ggacgttaag aatcacatga gcaccatgga agattcccag
    43081 gccagctggg tcagaaaaaa actgacggaa cctccggttc gcgagcatgc gcagaaaaaa
    43141 acggaggcag cgccccggca gaatgtcgca cctgcacaaa agacacccga accaccgccc
    43201 cggcccaaac ccaaaccgcc agcaactgac agcagtgcaa ctgtaacccg cggcaaagaa
    43261 ccaataaagc gtaccgatga tggcaggaag cagtcaccgc tggatgctgc ccgcccccag
    43321 gcaggcaaag agcccagacc ggcgccctca actgggcaag gtggcatgcg ggatagaagc
    43381 gggagaccca cacccccggc gcagaccggt catacgcctg cacccgggat taaaccccag
    43441 ggtcaatttg agaacagacc tgcacccggg attaaacccc agggtcaatt tgagaacaga
    43501 cctgcacccg gtgccagacc gcagggtcaa ttcgaccgca gaccggcacc cggggcaaga
    43561 aatgataatc gtccccggcc ccagaacgca ccaccgcgga cggaacagcg tccccaatca
    43621 gggactgcgc cgcagcgccg accggaaggc cagcaacccg gtaaacccgg tcctgttcgc
    43681 accgatgccg gaggcagacc cattcagcgc ccgcagcccg gacagaatca gaccaacaac
    43741 cgccctgcgg cacaggaaaa atcgaaccgc gttgcgcccg ccaacaaacc aggcggtgat
    43801 aataaaggac gcgctgctgc tcccggcaag ggttttaccc agaacaaagg cggaccaacc
    43861 gtaagacgtg actacagccg gccccagcgc aagggcaaac ataaacgcaa aaaagaagac
    43921 aacgttatgc tcacaccgga gtcaatcacc atcggagctt ccgttcaggt acgtgaattg
    43981 gctgaagcca tgggcaaaac tgctgccgaa gtggttaaaa aattaatgga attgggcacc
    44041 atggccacca ttaatcagga aatcgattat gatacagcgg agatcgtcgc cag
//
LOCUS       DAOTHZ010000009        39281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1098_length_39281_cov_7.908989, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000009 DAOTHZ010000000
VERSION     DAOTHZ010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 39281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39281
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_39281_cov_7.908989"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="P5549_02225"
     CDS             complement(<1..766)
                     /locus_tag="P5549_02225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRW11923.1"
                     /translation="MHDVVIVAAARTAVGDFQGSLTGLKGHELGVVALKGTMEKFGID
                     PNTIEEVIAGQCNQYGSPGNSGRWVTLKSGLPIECVSTTVHQQCPSSMRAADILSQEI
                     MLGRVNVAAAVGYESMSNAPYLLMDARKGYRLNDGERVQDSLMLGGLICPFEGYHMGI
                     TAENIAEMYGITREQQDEYALLSHQRACKAITDGLFKDEIIPVEVKIKKGIQIFDTDE
                     HPNANTSLEALAKLKPAFKKDGGTVTAGNASGLNDGG"
     gene            complement(789..1553)
                     /locus_tag="P5549_02230"
     CDS             complement(789..1553)
                     /locus_tag="P5549_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRW11924.1"
                     /translation="MDYKNFILDIEDSIAVVQINRPEALNALSRDMVLELEQVFITLE
                     ADDAVRVVILTGGKHFAAGADIKGMLDSNPEQARAFSFRHVFNRIEAFPKPLIGVVSG
                     FALGGGMELALVCDLRIADNTAKFGLPEINLGIFPGAGGTIRLPRLIGVGRAKEMIFS
                     GMPINAEQALQYGLINKIVDNPLEEAKNTARMLASKPPVALKHAKQCINMAFDTDAQK
                     AIEFESIAFSVCFATSDQYEGMKAFTEKRKPNFMGK"
     gene            complement(1583..2443)
                     /locus_tag="P5549_02235"
     CDS             complement(1583..2443)
                     /locus_tag="P5549_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRW11925.1"
                     /translation="MNMITKNICVIGAGLMGQQIVLQAAVCGFEASCVEINPEALKKA
                     QAFAEKWLQGRIDKNAMTPEAAQQVKDRLLYTTQLEEAAVKADLVIEAAVEDLGVKRS
                     LFAQLDKFCPEHTILSTNSSYIVSSKIADATKRPDKVLNLHFFNPALVMKLAEVVKGP
                     HTSDETVEEVLAVSRALNKVPTLVKKEIYGFVCNRIFSAITHEALYMLDQEVASIEDI
                     DNAIRNGLGHPMGPLQLLDMTGIDLEYKVSMDRYRETGDPADKPSPAIAERFALGHYG
                     RKSGKGFYQY"
     gene            2975..5137
                     /locus_tag="P5549_02240"
     CDS             2975..5137
                     /locus_tag="P5549_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW11926.1"
                     /translation="MLQTQKEDWVKLGEDLAHLGTRLNIKTSLKVKDFMRDELNMLSM
                     SASIESAMRIMVKDSIDAVPIVDEANNLVGLITKTLVLRKTLQGMDLVRPVSDIMIEK
                     VASIGPDEDVTKLISINVGNLPVVKNRQLLGMVTLSDTIRAYFSSLITLHAELNTVLD
                     STHNGILTVNAEREIILLNRAAEIALRIKREDVVGRKVGEILPQNRLTEALINGEVSF
                     GQKFTLGKKGYICNITPVHGNGQFIGTVGVFQDISELEMISEELSYTKEIKEELIALI
                     ESSFDGIHVTDNNGITLRANRAFCRITGWKNGDLEGKSMQELIDEGVYSQDIAAMVVM
                     RRESVTISQKSKPGNIVMITANPVFDERGVLFRIVINVRDISELNLLRQQLEQAQTLS
                     QHYQEKLSKYNLADHFVIRSKESRDLADLCVRLGQVDATVLIKGESGVGKEFAAQLIH
                     SNSNRRHKPMVTVNCAAIPESLFESELFGYEPGAFTGASKQGKRGLFEAADGGTLFLD
                     EIGETPLNTQAKLLRAIQEKVITRVGGTIPINVDVRIIAATNRNLQEMVQNKEFRSDL
                     YFRLNVIPVVVPPLRERKVEIPFLVDHFIQKFNAKYRLHKRIDEREIKAMMNYDWPGN
                     IRELENLIERLVVTYPGEVIRHINLQDIETTINNGVNYETFTGFKLQTAMEQLERQMI
                     KNALETLGSTRKAAMELGVSQPTIVRKAAKYNIPLREK"
     gene            complement(5205..5984)
                     /locus_tag="P5549_02245"
     CDS             complement(5205..5984)
                     /locus_tag="P5549_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRW11927.1"
                     /translation="MDYETLIVTKEDWLATIMFNRPKAMNASNQQSIADLTAAVADIE
                     ADDNILAVIMTGGDKVFAAGGDIKYFSEAGHLEMEAFIAKCHAVHDTMVASAKPYIAA
                     IGGLALGGGCEMALACDIRIAAENAIFGLPEINIGIIPGAGGTQRLPRVVGSGWARHL
                     ILTGDMIDAKTAFKIGLVTKVVPAEELMETAKKVAKSLARKSPMGIRSAKKCIAMSET
                     ADMPSALAYEQKAWAFLFSGSDRAEGMKAFLEKRKPTYINR"
     gene            complement(6149..6451)
                     /locus_tag="P5549_02250"
     CDS             complement(6149..6451)
                     /locus_tag="P5549_02250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRW11928.1"
                     /translation="MIVIVATFKAKAGMEKELEEGLVSVVPDVQQEEGTLMYTVHKSK
                     ADPGVFMFYEMYQDKAALEYHSSTPHFKALFGKIAPYLEDQPDIKIFKDIASINRK"
     gene            6695..8050
                     /gene="trkA"
                     /locus_tag="P5549_02255"
     CDS             6695..8050
                     /gene="trkA"
                     /locus_tag="P5549_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRW11929.1"
                     /translation="MKVIIIGAGKVGFSMAQLLSDEGNDVVIIEQSPERQQLLEEALD
                     VQVIYGSGSSTSVLEAAGVRSADMLIAVTEYDELNMIACLLAKKYGIKTTVARVRNPE
                     YLEVKGFSLNETLGIDLFINPERVTAKEIYKIVKNPEALSVEYYADGKVQLNELEIDE
                     ASHLKGKKLKELNSTIPYNIVAIVRDRKMLMPSGDDVLQEEDRINIMARTADMPEVEK
                     VMGFHSRKIEHVTILGGGRTGCYLAQMLEASRAGINIKIIERDLSRARQIAQKLKNTM
                     VIHGDGGDYEMLEEENIGHSDMFVAVTDDDKVNLLCCLIAQNLGVKKTICQIKRTDVM
                     PLVEQIGIDAVVSPRIITAGAILKYIRSGDIISVTLFGEERAEMLELLAQPGAVAVNK
                     TLKSLDMPNGALIGAVMRQDRVIIPDGNFIINPHDRLMVFALPRNIHKVEKLFRNGGR
                     K"
     gene            8051..9508
                     /locus_tag="P5549_02260"
     CDS             8051..9508
                     /locus_tag="P5549_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRW11930.1"
                     /translation="MIRGHVILNALGKISLFVGVAMLTCLLWASQDGETVIKGFVLAA
                     VVTIFTGLVLTIFFRTNATINFKESFAVVTLGWLVASLFGALPFLTTGVLDSFANAWF
                     ESMSGFTTTGASVFKDVEVCPRAVLFWRSLTQWLGGMGIIALFVALISNIGVRANQLF
                     KAEVPGPISDKISPRVRETARSLWKTYVVISLLCILTFYALGMNFFDAICHAFATMAT
                     GGFSTKNASIGFYSSPWLQWAVTFFMIVAGSNFALHFLALKRRNVDGYLKNAEFKFYL
                     AIIFVASLLIAMDLNHGLNGSLAAWEEKCRVACFQVVSILTTTGFATFDYTLWTPLAG
                     AVLFLMMFVGGCAGSTGGCIKPGRYLIILRRSLIELRKMVHPKAVMPLRFGGKVLNED
                     MVMNVQQFFFLYILSLVAGMMIISALGLDFVSSLTASAACLGNIGPGFGQVGPTQNFS
                     FLPAVGKYVLILEMLIGRLEIYPLLVLFVPAFWRR"
     gene            9765..10400
                     /locus_tag="P5549_02265"
     CDS             9765..10400
                     /locus_tag="P5549_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRW11931.1"
                     /translation="MMNLLDYLLLGWLAAGMIIGCKKGLVKVIGGLAGTLLGLAAAII
                     YRDDMALYLEREFGCRTMLVQTIAEKLPQPALGDIPLDTILPPLQSLPVIQNMLTDLA
                     QMILIVGAFLLLYLAVSKGILLLVKLLDMPLRKGALGGLNRLAGTVVMVGKELLVMTI
                     LLGIASPIIHNGAAMGLEGLMKAETALEQSYCAAWLQVFFAGLEKLVGIGV"
     gene            10463..11854
                     /locus_tag="P5549_02270"
     CDS             10463..11854
                     /locus_tag="P5549_02270"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRW11932.1"
                     /translation="MAEKKPKKHEDSWSRMNASEIKACEQFNAGYIDFLSQVKTEREA
                     VAYISTEAFRKGFKEIEQVKKIKPGDKLIIQLKSKVAALVVIGNQPLEKGVNLIASHI
                     DSPRLDLKPHPAYEADGMALFKTHYYGGIKKYQWLAIPLALHGVVVKKDGRIIEVSIG
                     EKADDPVFTIADLLPHLAKDQMERKMADAVKGEDLNAILGSRPLHDQEDEAIKTRVLN
                     ILKDQYEIEEDDFTSAELQFVPAFPARELGLDRSMIAAYGQDDRVSAYASLQAILAME
                     KPERTVLCLFADKEEIGSSGNTGLQSLLIENVFAELMHKTGQNDYFSLRKALANSAAI
                     SADVNAAVDPNYPEVFEKMNCSFLSRGVVLTKYTGSRGKYDSNDANPEFMAQIRKIFD
                     DAGVVWQVGELGKVDIGGGGTVAQYIARYGMEVVDCGVALLGMHSPFEVASKADVYMS
                     YKGYQAFLQKYLP"
     gene            11885..12937
                     /gene="selD"
                     /locus_tag="P5549_02275"
     CDS             11885..12937
                     /gene="selD"
                     /locus_tag="P5549_02275"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:11930..11932,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRW11933.1"
                     /translation="MGEGKRLTEMVRAAGUGAKIGPGALEEALKGLVLPQDPALLVGL
                     HTHDDAGVYKLNEETALVQTLDFFTPMVDDPYLFGQIAATNAINDVYAMGAKPLLAMN
                     VVCFPECEEMSLLAEILQGGLSKVKEAGALLVGGHTVDDNEPKYGLSVTGLIHPKKII
                     CNSAARPGDRLYLSKPLGNGVITTAIKAEMAEAGDVAEAIHWMTTLNQAGGEVMQQCG
                     VKAATDITGFGFLGHLYEMAAASNVEVEVYVQMIPFMGGALSYAGLGLIPAGAYNNRA
                     YLADKVLYEDEIEPDCRELLFSPETAGGLLMAVAAETEQEFLQRMEQKQVKVYLVGHV
                     LAEEFAPIRIRQRPAS"
     gene            12980..13195
                     /locus_tag="P5549_02280"
     CDS             12980..13195
                     /locus_tag="P5549_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRW11934.1"
                     /translation="MERKAFNLGDIVRMKKQHPCGSYHWEVTRMGADIKIKCQGCGRI
                     VMLPRSEFERKLTKVVESKPIPPEDQA"
     gene            13271..13483
                     /locus_tag="P5549_02285"
     CDS             13271..13483
                     /locus_tag="P5549_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11935.1"
                     /translation="MRLKMLDKHFSAGDLVTIKNSRKGEHFCIIGFKYDEDHQPMAIL
                     KALFNETCIIEKPVTELQTLLIKGKL"
     gene            13708..14001
                     /gene="rpsF"
                     /locus_tag="P5549_02290"
     CDS             13708..14001
                     /gene="rpsF"
                     /locus_tag="P5549_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRW11936.1"
                     /translation="MRDYEMMYIIKPDLADEAVAEIKEKLHKIIEDFGGEFENEVPGW
                     GKKRMAYRIEDYSEGIYVLWNFKGKPETVTELDRVIKISDSLLRHIIIRKDEK"
     gene            14040..14483
                     /gene="ssb"
                     /locus_tag="P5549_02295"
     CDS             14040..14483
                     /gene="ssb"
                     /locus_tag="P5549_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRW11937.1"
                     /translation="MNYNRVILVGRLTRDPELRYTTNGTAVASFSLAINRRFNREETD
                     FIDVVAWRQQAEYCANYGSKGRLMMVEGRLQVRTYEAQDGQKRKVTEVVSDDLKFLDK
                     QGTSQGSRPASTGKKDADGWDDLGREVRLEDIDIIDKPEEDDIPF"
     gene            14510..14734
                     /gene="rpsR"
                     /locus_tag="P5549_02300"
     CDS             14510..14734
                     /gene="rpsR"
                     /locus_tag="P5549_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRW11938.1"
                     /translation="MKKDKRKKRRQCNFCADKVECIDYKDLVKLRRYVTERGKILPRR
                     ITGNCAVHQRQLTVSIKRARIMALMPYTQE"
     gene            14860..15186
                     /locus_tag="P5549_02305"
     CDS             14860..15186
                     /locus_tag="P5549_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HRW11939.1"
                     /translation="MRSKIKAMNGESNLRIIEWLKAELIESTSVLFKSLLRSGGEATA
                     DALSTIVIICYVLGRRVGINYQSIDMNIMHKINTTVNEASEIEQMYGDLTELKKYLDR
                     KESRTR"
     gene            15191..16156
                     /locus_tag="P5549_02310"
     CDS             15191..16156
                     /locus_tag="P5549_02310"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HRW11940.1"
                     /translation="MPAVLKPFFITFAACLAIHLWPAGLFFWAVIWGTAILLAGAYMG
                     MIQLLALAGSNFLFLLLLGDSALTLDLLVFYAIPALVAAILLKKQKTYYTILGKALLA
                     AVMSVLIYMGISYAYLGTEGISQIKSGIDLAAENSLAWADQSGFMERYEQRGISREEI
                     KQAYTELAHLLLVFTPALLCIQALLATFLILYSSSRMYRKKRLSGLERKPFHEEIMPW
                     QLTWVVIAGLACWLLGREQMNWLYYTGTNILAIMFPITFFFGCAVIVYWLQHLPAKRK
                     TWVVTLVVLLGLFFSVPTIIFIGLTGLFDSLLDYRKVRRKKEGIQ"
     gene            16153..16599
                     /gene="rplI"
                     /locus_tag="P5549_02315"
     CDS             16153..16599
                     /gene="rplI"
                     /locus_tag="P5549_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRW11941.1"
                     /translation="MKVILTQDVKKQGVKGQIIEVSDGYARNYLIPQGLAEEASKTRL
                     KEIEEKKVHDAKQKDKEKAQAEQIKAKIDGQTIELKAKTGSNDKLFGAITSQEIADAL
                     KQKFGVKIDKKKIEQAEPIKHLGEYSFKIKIYPAVQAELKLIVTAE"
     gene            16618..18546
                     /gene="lonC"
                     /locus_tag="P5549_02320"
     CDS             16618..18546
                     /gene="lonC"
                     /locus_tag="P5549_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HRW11942.1"
                     /translation="MKQPMFKLFKRKRLQPEPTASLNSKIAILISQTEKLFGSQEFII
                     RASKLNAVELLQAEREEDQILGLHKILHEDPTLTALPAEKTGAQLLQELEELIAEQSA
                     RQMVARQMEQRVQELMEQNYNEYVQEIRKRVLKEANKSRENARTLKKLGQLEVMEQNG
                     LNDSALNFLRPGSMDEMVGQEQAVKALVSKLNSPWPQHILLYGPPGVGKTSCARLALE
                     MARSNPRSVFKANAPFIEVDGSTLRWDPRESTNPLLGSVHDPIYQGARRELAEDGIPE
                     PKLGLVAEAHGGILFIDEIGEMDHYLQNKLLKVMEDKRVYFESSYYDPQDERIPQYIR
                     KMFDEGVPADFLLIGATTRSREEIIPAFRSRCMEIFFEPLSAGQIDQIVHHSAVKLGI
                     RMEHEVSQIICDYSSDGRTANKILVDAYALARYEDDPETTGGIELRTRHVYEAIQNSR
                     ITPAIASKAYAGAEVGKIYGLGISGYRGTLIELEAIAFAAEKGNGSIRFNDTAGSMTK
                     DSVANAASVFRKIHHAQLKDYDVHINVVGGGQVDGPSAGAAIYLAISSAVLNCPVCQD
                     VAISGEISIQGRVKPVGGICQKILSARQAGVRKVLIPAENIADIPAGVKDIAIIPIRM
                     IEEAYEHVFAAESQAQVY"
     gene            18700..19971
                     /locus_tag="P5549_02325"
     CDS             18700..19971
                     /locus_tag="P5549_02325"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRW11943.1"
                     /translation="MSAVVLVGSQWGDEGKGKITDYLAEKADCVVRYQGGSNAGHTVE
                     VDSEKFMLHLIPSGILYPKTACIIGNGVVVDMGEVLKELQGLQERGIDTNNLRISSRA
                     QVVMPYHKEIDAIEDRCKKIGTTKRGIGPAYEDKISRTGFRICDLYDPGFKERFIQEM
                     ERKNLLLQKIYDAPGFAVETIYAELMEQAAQLKKYVADTSLLVNQVIKDGKKVLFEGA
                     QGTLLDIDHGTYPYVTSSYPTSGGACIGAGVGPTHINRVIGVVKAYTTRVGEGPFPTE
                     LNDDNGEILRTRGAEFGTTTGRPRRCGWLDAVILRYATRINGLTDLAITKLDVLDSFA
                     TIKICVAYRYQGELLYEFPDNLSILEECVPEYIEMPGWQEDITKIEQYEDLPSAARDY
                     IAKIEELSGVRQAIVAVGPKRSQTIVSHQLF"
     gene            20023..20097
                     /locus_tag="P5549_02330"
     tRNA            20023..20097
                     /locus_tag="P5549_02330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20055..20057,aa:Val,seq:cac)
     gene            complement(20150..21829)
                     /locus_tag="P5549_02335"
     CDS             complement(20150..21829)
                     /locus_tag="P5549_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW11944.1"
                     /translation="MSEKPWLKAFEEEVAKWRPVWHANYDDKIEAHPEYPTEPPKWWF
                     NKWAEEQPDKPYVMQGDVVITYGLANDLSRRLANALTNLGVKKGDRVAIMSPNLPQYV
                     LSIQALLKIGAIEVPSNPLYTVPELSYQFRDSGAETVIVMAMFANKAIEIMKEPNSPV
                     KRVIVFQVPTMPVEIEKADNIYDYNEIIGAASNAEPDVEITMDDIVRLQYTGGTTGVP
                     KGCVLTNFNVMSKAVRVGQWVTNNYTTMPGGDLRALACIPMNHIFGFMANVGFNFFCG
                     GSLVTVPVPNPDTLLDAIEKYQPATFPTVPAMIIAMLNHPRFKEADISCFKGGVFSGG
                     SPLPVEVMKSFEEQAHCRIVEGFGMSESCQQVCANSGHHTRKVGSVGVPYPDTDVLIV
                     DDKDGTTIMPVGEPGELIWRGPQMMREYWNNPEETALTIRDGWLYTGDIARMDEDGFF
                     FIVDRKKDMIICSGFNVFPRDIDEVCYAMPEVLDACTVGIPDPKRGETPKVFIVLKEG
                     ATLTAEEVIARCRENLAPYKVPSEVEFLQDLPRTLAGKADRKAMKAMALKK"
     gene            22420..23253
                     /locus_tag="P5549_02340"
     CDS             22420..23253
                     /locus_tag="P5549_02340"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRW11945.1"
                     /translation="MTVNINWKPSPNYTAGRGGRKIIAIVNHQTGGQGPGALSWLSNP
                     ASKVSAHYLIFRDGTVYQLVRLQDTAWQAGAVDQPSWMLYDGTNPNRYTLGIEHECYP
                     AVGGDGNLTERQYHATLELHRQLISNHSIVADRTHIIGHYQLDSVNRSNCPGRNFPWE
                     RLMQDLTLGTTSVPIVTSPKKLMGFVINGVSYAPVRALANQLGRTVEWDALRNAVLIP
                     PVTVAVPTAQADKVFVVTGAVVIEGLLLGGTSYAPARTLAEALGYQAEWDDANKTVII
                     L"
     gene            complement(23443..25362)
                     /locus_tag="P5549_02345"
     CDS             complement(23443..25362)
                     /locus_tag="P5549_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW11946.1"
                     /translation="MDYMEQPSYRWYMSQQTFPELLNRNVTQFAAKTAQMWKNQKGEV
                     ESLRYAQLGQVVKEVTCGLMALGVEPGDRVAIMSNTRPEWVWCDYGILCAAGITVCIY
                     PTLSANEMTYIMNDSGSKILFIEKQADLEKCLAASANIPSLEKLIIMDRDADFISDQA
                     MSLNKLREMGNVLLLQDRFAFEKRWRTVKLHDRMTIVYTSGTTGNPKGAVHTHFSINA
                     AVLRDLTFSPFIEDDYLLLSFLPISHTYERECGHALAMQTAIAVAYSSPQTLVADFAT
                     FRPHVFMSVPRIYERIYMALRASTSQSAVKKTIFNKAIDTGLQVVQTITDENGFIDVY
                     PHMDITRNASFFLKLKYRLYNKLVFNKVKEMMGGRFVFACSAAGSLSADLCKLFLAMG
                     LPIYEGYGSTETCNTINMNRVHRVLPGSVGALCPGVEGYMAEDGEWCVRGNNIFLEYW
                     NNPEATRNAFTEDGYYMTGDIVEMLPEGFIKIVDRKKGLIVLDTGKNIASSKVEQAFS
                     TSTYVDLAFAVGDSQKYIGALIVPNFEEFMALFEKNGVSYDQNAIKYIDVSGVKTVSS
                     VGDDFINQPLLKETVEKEVQRINNELESYEKIKKYAILPRRMTEESGEITPTLKVKRN
                     LVIQNFDTEIKSLFN"
     gene            25620..34874
                     /locus_tag="P5549_02350"
     CDS             25620..34874
                     /locus_tag="P5549_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW11947.1"
                     /translation="MLLINTCSLTAWADINTTKENSLNSNQNILQSLQTVQGEATTPE
                     AARQVLEELDLWDDSDSQQDLATVINWRGQAMTLDEIWKLINSPEVNLDEAATVDGTS
                     ITLRDLKTMIAIEYEIQRIKNTYFNDVNLDKEHQQTLSSLHQQIATEGIKIYPQGIVP
                     MATESSSLVFPSGIDQGIYVEVDASPLIYDNNSGEQTITARLKDRQGNALTQVPDYDI
                     SFDYRLLQGSAEFAYEFTGNRTGTVTFPAKSTAITQKITLRINDSIAWRHLPFEGQRS
                     FLVQFYNPHNVTFQEGKRLIEKVIKINSNYTFLPINDPFLNDSLPGYLALDYGSTVSF
                     AYDRNVLKNELFTQKVFNEIEVQLSGGTPFVGSPGNTSAYEFRPAIFGKYFGDVLTIK
                     SVNSIIDHFGTIALDDDVYDELIYDNDNQLDVYFGHNHTNPWDHCNVTAKVGLSLRFI
                     DTKNPCVKDFFRIDSKPWDVGKTFAYGQSIPICVTYSEPVDVSEATLQANGMTLTPIE
                     AGSHGVTSATFLYPVKQIDDPDLRISEPRGAKDLAGNLQVPSSDSVMESVTFKNIIQP
                     QYKLDTFKKASASLVTAENGVSVCQVEIDLNDNQDVTNWLVSEAGVNYDETVNKYRVP
                     SIYASLDGGATKIDLYADNDITPTKLVGSFVPAFNDSAVTRSGVVEFYLDPVIHSSTN
                     HQLLVGTYAVYTVEPVVFLTDDDLTISDNFPADGNLFADQANVWNLNYSVANTAATYQ
                     GADDFVWSSSDEAIAGITNDGRITLTGTPGSVTFTLTALNGQAAGKQVSLNSRTVIVK
                     AGLTPFLVIPEQMNAITIKQGEDVQVRWTSNLTGKNAEAGGSTVFDVDVSMADYRGGS
                     LRKGDSVYRSTVTSTQENPVSGCTIPAAYVGNISAMGRYSYIITITAPHPYDAAQTLT
                     AAAYLSVAAKPAVVRLGKLDKYFISDRTPSVPISWTLENFDEVNAAEFVLEISKNDGK
                     TPFYTQTGTDSSGGSYDLAIQTVDGGFKDLYTVAVKVRNKGDSAWSYDSFVLHVYSQD
                     ALHIWIDGAAPGTTFTMSNLPAISAMSSEEILALKRDIKLRNEASINFGDHGWGQISD
                     QIQWHSSDSRIGSLNYQQGGYYRNIEELIYSSYRPETKFALSGLHDGNTRLTVTHVAT
                     GLQRTLDVAVETLQDKLYLFQILPQAETTLTYVNGNGEQRVVTTNARGELALYEASGI
                     AGEIYLESAVEERIYMGTLYKGLLSSEKDSTQMAMYPLNLFKLRECATVELYVRKPDG
                     TPYHGEVFLNGGVYKNDRYCPTALLNKLPGDQSQTITTDQDGKIIVRMNTSQFWHESS
                     DEVLTGADKLNFIYLLHFPNDEYYPQIINLDGNMSSEEVVMSGAGIVSLEMVGEGEKY
                     KPFIAAQTITYETERDRRIDVRKFKGKIGPSDTHDKVILNTTVLWWGDSKDDPSEDRQ
                     LYFQDRYGMAFNNQTHTTDEYPFTDMVSSTNTFTLDRATVTEWSEPVQDLHLIYSHSG
                     STRYINQEMTFRAANMLDVPKAIDSDSLVITLQAMSANAKAEAGSLSLADEMIGAGLS
                     HLSQQGTGSLGEFFQMVIAPTADPTVYVALIALNLGGLATEDPQTGSELEYTPGFTDA
                     MDIMKKKYITEQKATLDKNRSGKSFSEKDFSFVFGGYMEAEVFYNFDHGKWEVLVLNG
                     GFNAGAGLDYTWNYNTMVGPIPVTAQLGVGASAEVIFKAAVQRGEIIKKTYDQDAVND
                     YLTTLRIYAYFKAFAGVGFDYSVVALKIGLFGRISLDAQFAWLNQPYAKTKKNGQSLT
                     VNGQIGIEADAKFLFVSETYILTSRNFKIAGATYNDWEKIQTTWEKIGKGSNDNLDRL
                     AIPNAMMPEDLSLYPISAQSTLEDRDYLQQFQRSWASSQEFVQQSLDEENGVDSLQTN
                     TYPFANPLFSDDGQILVYVYDGDSQEIENTRVYWSSLQDGQYPQGVPLAAEMTSFGDS
                     QLQLAGDDDFAAATWVTQSETIPREAGQEISNADIALLSNGTEVVASVYENRQWVTTR
                     LSQNSTPDMAPTVAVNDSRVLVAWRSVYAADADHPLEFNGADNILYRMYDREKKTWSE
                     TKTLYNGSSGAVKALEAAMLEDSTAALTYSLDTSADTAGSDHLLTVSDDGKKVSSGME
                     IIYAVIEPEGALGKNVRVTNDAFLDENPQLTAVTFDDDIERFVLGWYSIHDADGVSRN
                     DIRLCAFDRYGSIYPDFIDSISSVNNHSNVNISSRFKFSKNAPTIDQLSILWKEPANT
                     ATTEGDTVKADKDSLKAVKFIKENGKIFITSALDVATMSDFTLIDHFDARCVDQDTVK
                     AVILGTNYDNGYREHKIPIEVNGERTEQIIYLPVARSDLYTATAVYENKMSVNQVAVD
                     YRTIQPGLRIPVQFTLFNAGVQPIDNITIQIGNNITEFNQGLFLLPNESITLTATYDI
                     PTAQVINPDYNITAHFSSMMPTLGKQYTIADTLYLDVPDVGIANTKIVGEANGQRVVQ
                     VTLYNNGAALAGSSRNVKIGLFSDPECTHLLQMVNGQEEGVPFSVDPDDFALIDAGAY
                     TRQFIFDIAGHVMLGNEIPENGVQIFVKAWAEETLLTGDTAANLELIEYYRHNNISTV
                     LFESLLVRNDYQPVTLSTQLHCEEGSSTVLVDLKNNALASRHSGNLIVSLLDKNGRII
                     DTKRSYNRFLLGAGLLNLAGEESSQSVFTFDREGAAVMVTYADVNKEEEENTGLASHF
                     MQGIPLQLVEGQKEYYYETENLTATFINIAAQDPDAVIKVNGRPTDLGSAVVDLHHGI
                     NTITITVTSADGTVTDNYTVTISNSLQTPPQESGSHSGSGTATDAKGISITSEVKQGV
                     RLFSIKTDNQVLSAAGGRVNVVVPAENASPETVAIRVYPDGREEIIPMALLGNGQMII
                     SASEPMTIKLQENKPSFNDIQHHWGRDDILFVGARKLFAGIDHDKFGPDMTMTRGMLV
                     TVLGRLGNVPSERYKGSAFQDVSPDSYYAPYIDWAQQTGIVKGTGDNRFAPDEAITRE
                     QLAVMLANYVAFSQLGLKNPIDKKPFVDDEKISDYARDAIESMHQAGIINGRDDQRID
                     PQSTASRAEVATMLARLIRNVMQQ"
     gene            34913..36169
                     /gene="uvsE"
                     /locus_tag="P5549_02355"
     CDS             34913..36169
                     /gene="uvsE"
                     /locus_tag="P5549_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HRW11948.1"
                     /translation="MSIGYACLTIGVQNTDLKTCMLKNVNESRLAELISHNLQSLERI
                     IDYNLANRIRLFRISSDLIPFGSSPANTLVWPEIFTARLQTIGAKINDSGMRVSMHPG
                     QYTVLNSPDAGVVARAIEDLAYHAQVLESLGTGSEHKIVLHIGGVYNDKKQAIKRFIA
                     HYRQLDSAITRRLVLENDERSYNIEEVLEIGTKLGAPVIFDNLHHAIHPPAGEKDQFY
                     WLEQCRPTWHPRDGQQKIHYSQQNPGKKPGSHSDTIHINEFLRFYHTLNRNDLDIMLE
                     VKDKNLSALKCINCTLAEKKIKQLEQEWSTYKYTVLERSPSAYAQIRQLLRDKNNYPA
                     AAFYSLLEDALQSPLETGHAINAAQHVWGYFKDVASEKEKASFFQILNDYQQGKTGLP
                     KTKSFLKKMADKYQRRYLLDSMYFIL"
     gene            36243..37043
                     /locus_tag="P5549_02360"
     CDS             36243..37043
                     /locus_tag="P5549_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRW11949.1"
                     /translation="MVNQKEVTSIKMLVLVTYLAMIIVNALANILPINGVTTGQVSDS
                     YPNLFAPAGSTFAIWGLIYLLLALYCLYQMGLFRDPQAPRNQALLARTGLIFSISSLA
                     NVAWIFSWHNYMIAFSMLWMIVILLCLITILQTIRQETLTRREKFLIKLPFSVYFGWI
                     TVATIANATTLLVSWQWNGWGIAEPVWAIIIIIVGLLIGAATTIINRDIAYGLVIVWA
                     YIGIYIKHTSLAGFSGQYPAIINTVLACIGGMLAVLVYVYIQVKRPMH"
     gene            complement(37119..38465)
                     /locus_tag="P5549_02365"
     CDS             complement(37119..38465)
                     /locus_tag="P5549_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW11950.1"
                     /translation="MSDIIKWQYEQKKITAEQAAAMVKSGDQVAYGEFTMFPLALDEA
                     LSKRVNELQDVRVTGVCYTSPPQVVLADPKREHFILTDLHFGIVSRKLHDHNLCNYIP
                     NTYHQGSRIIKKYYNIDVGFFSVSSMDARGYFNLGPSNSITPAIMEKTKIKIVEVNNT
                     VPVCLGGNQESIHISQVDYVVEGNNIPLRQLTPAMPTENEQTIANLLIKEIEDGACLQ
                     LGIGGLPNAVGALIADSDLKDLGIHSEMMMDACVDLYDKGLITGAKKTIDRNKMVYTF
                     ALGTQKLYDFLDNNPVCAIYPVNYTNDPRVIALNDKVVAINNALAIDLFTQVASESVQ
                     TRQVSGTGGQLDFIMGAFNSHGGKGIICIDSTYTDKEGNLHSRIMPTLAPGTIVTLPR
                     SLVQYVATEYGIEQFKGKSTYERAEGLIRLAHPQFRDELIKAADEMKIWRRTNKIE"
     gene            38678..38965
                     /locus_tag="P5549_02370"
     CDS             38678..38965
                     /locus_tag="P5549_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRW11951.1"
                     /translation="MFGITHHEMWFIYLLAVNTVCLLLFFIDKRRARAGRYRIPERTL
                     FLWAVIGGAAGGWLGMYLFRHKTRHGKFTFGFPLLLLLQGVLIYFITRTGI"
     gene            39110..39185
                     /locus_tag="P5549_02375"
     tRNA            39110..39185
                     /locus_tag="P5549_02375"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39143..39145,aa:Asp,seq:gtc)
BASE COUNT        10342 a         9111 c        10110 g         9718 t
ORIGIN      
        1 cgccaccatc atttagtccg gatgcattgc cggcagtaac agttcccccg tctttcttga
       61 aagccggctt taacttggcc agtgcttcca aactggtatt ggcattggga tgctcatcgg
      121 tatcaaagat ttggatgcct ttcttgatct tcacttcgac cgggatgatt tcatctttga
      181 acaatccgtc agtgatcgcc ttacaagctc gctgatgact gagcagtgca tattcatcct
      241 gctgctcccg ggtaatgccg tacatttcgg caatattttc ggcggtgatg cccatatggt
      301 aaccttcgaa tggacaaatc agaccgccca acatcaaact gtcctgtacg cgttcaccgt
      361 cattcaggcg atagcccttt ctggcatcca tgagcagata aggggcattg gacatgcttt
      421 catagccgac ggcggcagcg acgttgaccc gtcccagcat gatttcctgg gaaagaatat
      481 cagccgcccg catggaagag gggcactgct gatggaccgt tgtggaaaca cactctatgg
      541 gcagacctga ctttaacgtc acccagcggc ctgaattgcc gggagatcca tattgattgc
      601 actgaccggc aattacttct tcaatggtgt tgggatcaat gccgaatttc tccatcgttc
      661 ctttaagggc gacaaccccg agctcatggc ctttcagacc tgtgagcgaa ccttggaaat
      721 ctccaacggc ggttctggcg gcagcaacaa tgactacatc atgcatatta caggctcctt
      781 tcaatgggct atttacccat aaaatttggt ttgcgttttt cggtaaatgc tttcatgcct
      841 tcgtattgat cactggtggc aaaacagacg gaaaacgcga tcgattcaaa ttcaatcgcc
      901 ttctgcgcgt cagtatcaaa ggccatgttg atacactgtt tggcgtgctt taaggcaacc
      961 ggtggtttgc tggccagcat gcgagcggta tttttggctt cttccaaagg attgtcaacg
     1021 attttgttga ttagtccgta ttgcagggct tgctctgcat ttatgggcat accgctgaaa
     1081 atcatttcct tggcacgacc gacaccgatt aatcttggca aacgaatggt acctccggcg
     1141 ccggggaaaa tgcccagatt gatttccggc agaccgaatt tggcagtatt gtcggcgatt
     1201 cgcaaatcac acaccagtgc caattccatg ccgccgccca aggcgaaacc ggaaacaaca
     1261 ccgatcagcg gtttgggaaa agcttcgatg cggttgaaaa cgtgccggaa agaaaatgcc
     1321 cgcgcctgtt ccggattgga atccagcatt cctttaatgt cagcaccggc agcaaaatgt
     1381 ttgccgccgg tgaggatcac cactcgcacc gcatcgtctg cttccaaggt gataaatact
     1441 tgttccagtt ccaggaccat atctctgctt aaagcgttca aggcttccgg tcggttaatc
     1501 tgaacgaccg caatgctatc ctcaatatca agtataaaat ttttgtaatc catgattttt
     1561 acccccaagt tttttcgtgc ccttaatact ggtaaaaacc tttaccggat ttgcgcccat
     1621 aatgccccag agcaaatctt tcggcaatcg ccgggctggg tttgtcggcc ggatcaccgg
     1681 tttcccgata acggtccatg gatactttgt attccagatc gatcccggtc atatccagca
     1741 attgcaaggg tcccatggga tggccgagac cgttgcgaat ggcattgtcg atgtcttcga
     1801 tcgaagccac ctcctgatcc agcatataga gcgcttcatg ggttatggca ctaaaaatgc
     1861 ggttgcaaac aaaaccatag atttcttttt taaccagggt gggaaccttg ttcagggccc
     1921 ggctgactgc caaaacttcc tccacggttt catccgaggt atgggggcct ttgacaactt
     1981 ccgccagttt cataaccaga gccggattga aaaagtgcag attaaggacc ttgtccggtc
     2041 gcttggtggc atcagcaatt tttgaactga cgatatagga actgtttgtg ctgagaatag
     2101 tatgttcggg acagaacttg tccagttggg caaacaaact gcgtttcacg cccagatctt
     2161 caaccgccgc ttcaattacc agatcagcct tgaccgcagc ttcttccagc tgggtggtgt
     2221 ataagagccg atctttgact tgttgcgcag cttcgggggt catcgcattt ttatcaatgc
     2281 gtccctgcaa ccatttttcc gcaaaagctt gcgccttttt cagggcctcc ggattaattt
     2341 ccacacaact ggcttcaaat ccacaaactg cagcctgtaa gacgatctgc tgacccatta
     2401 gtccagcgcc gattacgcaa atgtttttgg tgatcatgtt catcctcctt aacaaattag
     2461 ttgcttttaa attagctgtt atgcgggttc tgcttgaatc aggttgaaat atagcgctgc
     2521 cattgtttct atttagatga acttcgcaaa aatcatgcca tcggatgaaa atccccagat
     2581 aaggggcctt tttgttcgag tatcaataat atttacaaat tttgcgttcc cttttggcaa
     2641 aacaatgtgc tatatttgat acaatagtgt atcaaaaggc tgctcgccag ttcaatcaaa
     2701 gcagtattcg cgataatagt tcgttaatat gcgtattggc ggaacaactg caatcgatgc
     2761 aatgctgtat caatatgaca gcattcctct tgtaaccggg atataatcag ccacaaaccc
     2821 ttgccaagcc gggaacagat acggtttttc aaggatttgc cggggcgatt ttgttggtga
     2881 tacattattg tttcatttgt ctccgcccgg gaacagttaa ctccccgcaa gggaagagcg
     2941 aaaccttggt cagataaaaa gggggttgct gacattgctg caaacacaga aggaagactg
     3001 ggtaaagctg ggtgaggatc ttgcccatct gggcacaagg ctcaatatca aaacgtcttt
     3061 gaaagtcaag gatttcatgc gtgatgagct gaatatgctg agcatgtcgg ccagcataga
     3121 gtcggctatg agaatcatgg tcaaggacag tattgatgct gtaccgattg tggatgaagc
     3181 caataatctg gtgggtttga tcaccaagac cctggtgctg cgcaaaacct tgcaaggcat
     3241 ggaccttgtc cgaccggtca gcgatattat gatcgaaaag gtagcctcta tcggaccaga
     3301 tgaagatgtg accaaactga tatcgattaa cgtgggtaat cttccggtgg taaaaaaccg
     3361 tcaactgctg ggcatggtta ccttgtcaga taccatccgc gcctatttca gctctttgat
     3421 taccctgcat gccgaattga atacggtcct tgacagcact cataatggaa ttctgaccgt
     3481 caacgcggaa agagaaatta ttctcctcaa tcgggcggca gaaatcgctc tgcgtatcaa
     3541 gcgggaggat gtcgtgggac gcaaggttgg tgaaattctg ccgcaaaacc gattgaccga
     3601 ggcgttgatc aatggagaag tcagtttcgg gcaaaaattt acccttggta aaaagggata
     3661 tatctgcaac atcaccccgg tgcacggcaa cgggcagttt ataggaaccg tgggggtatt
     3721 tcaggacatc tctgaattgg aaatgatttc tgaggaactg agttatacca aggaaatcaa
     3781 ggaagaattg attgcgctga tcgaatcttc ctttgacggg attcacgtta ccgacaataa
     3841 tggcataacc ctgcgggcca atcgtgcctt ttgccgtatt accggctgga agaacgggga
     3901 cctggaaggc aagagcatgc aggaattaat tgacgaaggg gtttattccc aggatatcgc
     3961 cgccatggtg gtaatgcgcc gcgaatccgt taccatttcg cagaagtcaa agccgggcaa
     4021 tatagtcatg attaccgcca acccggtttt tgacgaacgg ggcgtgttgt ttagaatcgt
     4081 tatcaatgtt cgcgatattt ccgagttgaa tttgctgcgt caacagctcg agcaagccca
     4141 aaccttgagt cagcattacc aggaaaagct aagtaaatac aatctggcgg atcattttgt
     4201 aattcgttcc aaagaatcca gggatctggc cgatctgtgt gtacggctgg gacaggtcga
     4261 tgctacggtg ctgattaaag gggagtctgg agtgggtaag gaatttgctg cccagttgat
     4321 ccattccaac agcaaccgcc gccacaaacc gatggttacc gtcaactgtg ccgccattcc
     4381 ggaaagcctg tttgaatcgg aattgttcgg ttatgagccg ggcgcattta caggggccag
     4441 caaacagggg aaacggggtt tgtttgaagc ggctgatgga ggcaccttgt ttttggatga
     4501 aatcggggag actccgctca atacccaggc caagctgtta cgggcgatcc aggaaaaagt
     4561 gatcacccgg gtggggggaa ccattcccat taatgttgat gttcgcatca ttgctgccac
     4621 caaccgcaat ctgcaggaga tggtacagaa caaggaattt cgcagcgatc tgtatttccg
     4681 attgaatgtc attcccgttg tggtgccgcc gttgcgggag agaaaggtgg aaataccatt
     4741 tctcgtcgat cattttatcc aaaaattcaa tgccaagtat cgtctgcata aacgcatcga
     4801 cgagcgggaa atcaaggcca tgatgaatta tgattggccg ggcaatatca gggagttgga
     4861 aaacctgatt gaacgtctgg ttgtcaccta tcccggcgaa gtcatccggc atatcaatct
     4921 gcaggatatt gaaaccacga ttaataacgg agttaattat gaaacgttta ctggattcaa
     4981 actgcagaca gccatggaac aattagaacg gcagatgatc aaaaatgcct tggagacgct
     5041 gggatcgacc cgcaaggcag ccatggaact gggggtaagc caacccacca ttgtgcgcaa
     5101 ggcggccaaa tataacattc cactgcggga aaaatagcca ccgttaaaag caaaaaggcg
     5161 atcgcaccat ttttgggcga tcgccttgat ttacttggct aaaattatct gttgatgtaa
     5221 gtgggttttc tcttttcgag gaaagctttc attccttctg ctctgtcaga tccggagaaa
     5281 aggaatgccc aggctttctg ttcgtacgcc aatgcagaag gcatatcagc agtttcgctc
     5341 atggcaatac atttcttggc actgcggatg cccatggggc tctttctggc caaactttta
     5401 gcgactttct tggcggtttc catcagttct tcagcgggaa caactttggt taccaggcca
     5461 attttaaaag cggttttggc gtcaatcatg tcgccggtga ggatgaggtg tctggcccag
     5521 ccggagccaa ctacacgcgg taaacgctgg gttccgccag cgccggggat gatgccgatg
     5581 ttgatttccg gcagtccgaa aatcgcattc tcagcagcga ttctgatgtc acaggccaag
     5641 gccatttcac agccgccacc caatgccagt ccgccgattg ctgcgatgta aggtttggct
     5701 gaagcaacca tggtgtcatg cactgcatgg catttcgcga taaaagcttc catttccaga
     5761 tgcccggctt ccgagaaata cttgatgtcg ccgcctgctg cgaaaacttt atcgccgcca
     5821 gtcataataa cagccaggat attgtcgtct gcttcaatgt ccgcaacggc cgccgtcagg
     5881 tccgcaatgg actgctgatt ggaagcattc atggctttgg ggcgattgaa catgatggtg
     5941 gctagccagt cctctttcgt aacgattagg gtttcgtaat ccattcttac atcctccttc
     6001 agtaattagc ttttccgatt attatcggag cctttacgtt aatgatagcc ggttctttcc
     6061 aatctgtata gtacttttat aaacaaaatt agcattacat gaaaacactg gcaaatgcaa
     6121 aaacctctat attcgggctt tggtttgatt attttctgtt aatggaagca atatccttga
     6181 agatttttat atccggctga tcctccagat aaggagcgat tttgccaaac agagctttga
     6241 aatgcggagt tgaactgtga tattcaagcg ccgctttatc ctggtacatt tcgtagaaca
     6301 taaacactcc gggatcagct ttggatttat gcacggtgta catcaaggtt ccctcttcct
     6361 gctggacatc gggaacaacg ctgaccaaac cttcttccag ttctttttcc atgcctgctt
     6421 tggccttgaa agtagctaca ataacgatca ttttcatacc tcccgtttat atttgccaca
     6481 gccgcaaaat tttatgctcg tcagctgtta tatctttatt atcatattct tggccgggta
     6541 ttgcaaatct ccggaaaacg accatgtaac attattcaca aagtatttaa aaatatatat
     6601 ccaatggata taatcagtat gttacaggaa aattatttaa aaaaggccgg tgcaaagtca
     6661 ttcggcctat catcattcat gagggtggtt agcgatgaag gtgatcatta tcggtgctgg
     6721 taaagtcggc tttagtatgg cgcaattgtt gtcagatgag ggcaatgatg tggtgatcat
     6781 tgagcaatcc ccggagcggc agcagctgtt ggaagaagcc ctggatgtac aggttatcta
     6841 tggcagcggt tcgtctactt cggtcttgga ggctgccggc gttcgcagtg cggatatgct
     6901 gattgccgtt acagaatatg atgaactgaa tatgattgcc tgcctgctgg ccaaaaaata
     6961 tgggatcaaa acaaccgtag cccgggttcg caatcctgaa tacctcgaag tgaaagggtt
     7021 ttccctcaat gagacgttgg ggattgatct gttcattaat ccggaaaggg ttacagccaa
     7081 ggagatttat aaaattgtta aaaaccccga ggcgctcagt gtggagtatt atgccgacgg
     7141 caaggtgcag ttgaacgaat tggaaattga tgaggcgtcg caccttaaag gtaaaaaatt
     7201 gaaggaactg aacagtacca tcccttataa tattgtagcc attgtacgag atcgcaaaat
     7261 gctgatgccc agcggggacg acgttttgca agaggaagac cggattaata ttatggcccg
     7321 cacagcggat atgccggagg tagaaaaagt catgggcttt cattcccgca agatcgagca
     7381 cgtgaccatc ctgggcggcg gacggaccgg atgctatctg gctcagatgc tggaagccag
     7441 ccgcgccggg atcaatatta aaattattga aagggattta tcccgggccc ggcagattgc
     7501 acagaaatta aaaaacacca tggtcattca cggagacggg ggtgactacg aaatgctgga
     7561 ggaggaaaac attggtcatt ccgatatgtt tgttgccgtt actgatgacg acaaagtgaa
     7621 cctgctttgt tgcctgatcg cccagaatct gggggtaaaa aagaccatct gccaaataaa
     7681 acgaactgac gttatgccgc tggtcgaaca aattgggatt gatgccgttg tcagcccgcg
     7741 gattattact gcgggggcga ttttaaaata tatccgcagc ggtgatatca tctcggtgac
     7801 cctgtttggc gaagaacggg cagagatgct ggagctattg gcgcagcctg gagcggtggc
     7861 tgtcaacaag acgttgaaga gcctcgatat gcccaatggc gcgctcattg gtgcggttat
     7921 gcgccaagac cgggtgatta ttcctgacgg caattttatc atcaatcccc atgataggct
     7981 gatggtattt gccctgccca ggaacattca caaagtagag aaattgtttc gcaacggggg
     8041 gcgcaaataa atgattcggg gacacgtgat cctgaatgct ctgggcaaaa tcagcttgtt
     8101 cgtgggggtg gccatgttga cttgtctgct ctgggcaagc caggacgggg aaacggttat
     8161 caaaggtttt gttctggccg cggtcgtgac catttttacc ggattggttc tgacaatttt
     8221 tttccgtacc aacgcaacta ttaatttcaa agagagtttt gcggtagtga ccctgggctg
     8281 gctggtggcg tccctgtttg gggcattgcc ttttttaacc accggagtgc tggattcatt
     8341 tgccaatgcc tggtttgaat cgatgtcagg ttttacaact accggagctt cggtattcaa
     8401 ggatgtggaa gtatgtcccc gggcggtgct gttttggcgc agcttgaccc agtggctggg
     8461 tggaatgggg atcattgctc tttttgtagc gctcatctcc aatatcgggg tacgggccaa
     8521 ccaattgttt aaagctgagg taccaggacc gatttctgac aagatcagcc cacgagtccg
     8581 tgagacggcc cgcagtctct ggaagaccta tgttgtcatc agcttgcttt gtattttgac
     8641 cttttatgcg ctggggatga atttctttga tgcgatttgc catgcttttg cgaccatggc
     8701 caccggagga ttttcgacca aaaatgccag tatcggcttt tacagcagtc cctggctgca
     8761 atgggcagtt acgtttttca tgattgtggc cggtagcaac tttgccctgc attttctggc
     8821 actgaaaaga cgtaatgttg atggttatct aaagaacgcc gaattcaagt tttatttggc
     8881 catcattttt gtggcgtcac tgttgattgc catggatttg aatcatggtc tgaacggatc
     8941 gctggcagcg tgggaagaga aatgcagagt ggcctgcttc caggtggttt ccatacttac
     9001 cactacagga tttgctacgt ttgattatac tttgtggacc cctttggcgg gcgcggttct
     9061 ttttctgatg atgtttgtcg gcggatgtgc cgggtctacc ggtggatgca ttaaaccggg
     9121 cagatatttg atcatcttgc gccgttccct gatcgagctc aggaaaatgg ttcatcccaa
     9181 agcagtcatg ccgctgcgat ttggcggcaa agtgctgaat gaagatatgg tgatgaatgt
     9241 acagcagttt tttttcctat atatactaag cctggtggcg ggaatgatga tcatcagtgc
     9301 gttgggactg gattttgtca gcagcctgac ggcttcggca gcttgtctgg gcaatatcgg
     9361 acccggtttt ggacaggttg gccctaccca gaacttcagc tttcttccgg ctgtcggcaa
     9421 gtatgttttg atcctggaaa tgctgatcgg tcgtctggag atctatccct tgctggtctt
     9481 gtttgtgccc gccttctggc gccgataaga accccacccg ctagcgcaat caatagattg
     9541 cttgcgggtc ccgggaacct ttttgctgcg caataagaac cccacccgct agcgcaatca
     9601 atagattgct tgcgggtccc gggaacctac ttggttcacc gcaaaggact gcctgcacag
     9661 gctgcgtttc ttctgcattc aagtcaaccg ctggtgagaa aaatatgata cgctataaga
     9721 ggtgtgtatt ccatcagctg aaaattgacg gttactgagg agagatgatg aacctgctgg
     9781 attatttgct gttaggctgg ctggcagccg gcatgatcat tggttgtaaa aaaggcctgg
     9841 tcaaagtcat cggaggatta gccggaacgc tgcttggatt ggctgctgcg attatttatc
     9901 gggatgatat ggcactgtac ctggaaaggg aattcggttg ccgtaccatg ttggtgcaga
     9961 ccatcgccga aaaactaccc cagcctgcgt tgggagacat tcccctggac acaattctgc
    10021 ccccgctgca gtcattgccg gtcattcaaa acatgctgac ggacctggcc cagatgattt
    10081 tgatcgtagg tgctttttta ttgttgtatc tggcggtcag caaaggaata ctgttgcttg
    10141 taaagttact ggatatgcct ctgcgcaagg gagcgttggg cgggttaaac cgtctggccg
    10201 gaacggtggt catggttggc aaggaattac tggttatgac gatcttgctg ggaatcgctt
    10261 ccccgattat tcataacgga gcagctatgg gactggaagg cctgatgaaa gcagagacgg
    10321 cactggaaca atcctattgc gcggcttggc tgcaagtttt ttttgccgga ctggaaaagc
    10381 ttgttggaat cggtgtatag tttgccggtc ggattgagaa attaggaata atattatata
    10441 gaaggttgaa aggagtctgg caatggcgga aaaaaaacca aagaaacatg aggatagctg
    10501 gagtcggatg aatgccagtg aaataaaagc ctgcgagcag tttaacgcgg gctatatcga
    10561 tttcctcagt caggtaaaga cggaacgtga ggcggttgcc tatatcagta cggaggcttt
    10621 ccgcaaggga tttaaagaaa tagagcaggt caaaaagatt aagcccgggg ataaattaat
    10681 cattcaatta aagtccaaag ttgcagcctt ggttgtaatc ggcaatcaac cgctggaaaa
    10741 aggcgtaaat ctgatcgctt cgcacattga ttccccgcga ctggatctaa agcctcatcc
    10801 ggcctatgaa gcggatggaa tggctttgtt caagacgcac tattacgggg gaattaaaaa
    10861 ataccaatgg ctggccatac cgctggcgtt gcacggagtc gtggtgaaaa aagacgggcg
    10921 catcatcgaa gtcagcatcg gcgaaaaagc agatgatccg gtatttacca tcgccgatct
    10981 gctgccccat ctggccaagg atcagatgga gagaaaaatg gcggatgcgg tcaagggtga
    11041 agacctcaat gcaatactag gcagccggcc cctgcatgac caggaggatg aggcgatcaa
    11101 aacccgggtg ctgaacatac taaaggatca atatgagatt gaagaagatg attttacgag
    11161 tgccgaactg cagttcgtac ctgcctttcc ggctcgggaa ctgggactgg accggagcat
    11221 gatcgcagcc tacggtcagg acgaccgggt tagcgcctat gcttcgctgc aggcaatcct
    11281 ggctatggaa aaaccggaaa ggactgtact gtgcctgttt gcagacaagg aagagatagg
    11341 cagttccggc aataccggcc tgcaatcctt gctgatcgaa aatgtttttg cggaattgat
    11401 gcacaagacc gggcaaaatg actattttag cctgcgcaag gccctggcca attcggcagc
    11461 catttcggca gatgtaaatg cggcagtgga tcccaactat ccggaagtat ttgagaaaat
    11521 gaattgcagc tttctttcac ggggcgttgt attgacaaaa tacaccggtt cacgcgggaa
    11581 atatgattcc aacgatgcca acccggaatt catggcgcag attcgcaaga tttttgatga
    11641 tgccggtgtt gtttggcagg ttggtgaact gggcaaagtg gatatcggcg gaggcggcac
    11701 tgtagcccag tatattgccc gctatggaat ggaagtggtg gactgcggcg tagccctgct
    11761 gggcatgcat tcaccttttg aagtggccag caaagctgat gtttatatgt cctataaagg
    11821 gtatcaggcg tttttgcaga aatatctacc ataatcattc tggcgcaggg ggaggtgaca
    11881 aacaatgggt gagggaaaaa gacttacgga aatggtgcgg gcagccggct gaggtgccaa
    11941 aatcgggccg ggggccctgg aagaagcatt aaaagggttg gttttgccac aggatccggc
    12001 gttgctggtg ggtttgcata cccatgatga tgccggggta tataaattaa atgaagaaac
    12061 agcccttgta cagaccctgg atttttttac gcccatggtg gatgatccct accttttcgg
    12121 acagattgct gccaccaatg ccatcaacga tgtctatgcc atgggcgcca aaccacttct
    12181 ggcgatgaat gtggtttgct ttccggaatg tgaggaaatg tccctgctgg ccgagatcct
    12241 gcaaggcggg ctgagcaaag tcaaagaagc cggagcgctg ctggtgggcg gacatacggt
    12301 ggatgacaac gaacccaaat atggtttgtc cgtcaccgga ctgattcatc ctaaaaaaat
    12361 catctgcaac agtgcggctc gtcccggtga tcggctttat ttaagcaaac cactcggcaa
    12421 tggagtgatt accactgcaa ttaaagcgga gatggcggaa gctggggatg ttgccgaagc
    12481 catacactgg atgaccaccc tcaaccaggc aggcggggag gtcatgcagc aatgcggggt
    12541 caaagcagct actgatatta caggctttgg attcctgggt cacctttatg aaatggctgc
    12601 ggccagcaat gttgaggtag aagtgtatgt acaaatgatt ccgtttatgg gaggcgcact
    12661 cagctatgca ggattgggtc tgatcccggc cggcgcctat aacaaccgcg cttatctggc
    12721 agacaaagtc ctctatgaag acgaaattga accggactgc cgggaattat tgttctcgcc
    12781 ggagaccgct ggagggttgc tgatggcagt agcggccgag acggagcagg aatttttaca
    12841 acgcatggaa caaaagcaag tcaaggttta tctggttggg catgtattgg cagaggagtt
    12901 tgcaccgatt cgcatacggc agcgaccagc gtcgtgaaga ggctcaaata taaaggatgc
    12961 agggttttgg gggaatgaga tggagaggaa agcatttaat ctgggcgaca ttgtgcgcat
    13021 gaaaaaacag catccctgtg gcagctacca ttgggaagtg actcgcatgg gcgctgacat
    13081 taaaataaag tgtcagggtt gcggccggat tgtcatgttg ccccgcagcg aatttgaacg
    13141 aaaattgacc aaggtggttg agagcaaacc catcccgccg gaagatcagg cataaagcag
    13201 ctgggccctt gcacccgccg gcgcaggcgt gcatatactg tagcacaatg atgccccagg
    13261 gggtgtgaat atgcggctga aaatgcttga taaacatttt tccgctggcg atctggttac
    13321 catcaagaat tcccgcaagg gagaacattt ctgtatcata ggctttaaat atgatgagga
    13381 tcatcagccc atggccattc tgaaagccct ctttaatgag acctgcatca ttgaaaagcc
    13441 ggtcacagaa ttacaaacgc tgctgattaa gggaaagctg taagtgccca acggttatgc
    13501 aggaaagcgc tcctcagtaa acacgacctt tttcttccaa ccgggatgca tttacccaca
    13561 tcgatgtaaa aaaatgttaa atttttgcca aacttattgc atctcaactt taatcctgct
    13621 ataatctatg gaagtccctg ccccggattt catttcgggg ccattactcg tgttgacgag
    13681 tttagtccag agggaggagg tgtaaaaatg cgcgattacg aaatgatgta tatcatcaag
    13741 cctgaccttg ctgacgaagc ggttgctgaa atcaaggaaa aactgcacaa gattatcgaa
    13801 gattttggcg gtgaatttga aaacgaagtt ccaggttggg gaaaaaagcg catggcgtac
    13861 aggatagaag actattcaga gggtatctat gtcttgtgga atttcaaagg caagcctgaa
    13921 acagtcactg aactggaccg ggttattaag atatccgaca gcctgttgcg tcacataatc
    13981 attcgcaaag atgaaaaata gatgatgtgc gctcggtttt ttgatagggg gagtctctga
    14041 tgaattacaa tcgggttatt ctggtaggcc gtttgacgcg tgatcctgaa ttgcgatata
    14101 caaccaacgg gacagccgta gcttcgtttt ccctggccat caaccgcaga ttcaatcggg
    14161 aggaaaccga ttttattgat gtggttgcct ggcgtcagca agctgaatac tgtgccaact
    14221 atggtagcaa gggccgcctg atgatggtgg aaggcagact gcaggtgcgc acctatgaag
    14281 cgcaggacgg gcaaaaaaga aaggtcactg aagtggtctc cgacgatctg aagttcctgg
    14341 ataaacaagg gacaagccag ggcagtcgtc ctgcttcaac cggaaaaaaa gatgccgatg
    14401 gctgggacga tctgggtcgt gaagttcgcc tggaggatat cgatataatt gataaacccg
    14461 aggaagatga catccccttt tagcagctta gcagaaggag gtttgcagct tgaaaaaaga
    14521 taaaaggaaa aagagacgcc agtgcaattt ttgcgctgat aaagtagaat gcatagatta
    14581 caaggatttg gttaaactgc gccgttatgt gacagaaaga ggcaagatcc tgccccgcag
    14641 aattaccgga aactgtgcgg tgcatcagcg tcagctgacc gtgtccatca aaagagcacg
    14701 gattatggct ttgatgcctt atacccagga ataaaagaca ggccgccctc ctgcatgcag
    14761 gagggctttt ttactataaa aaccagaaaa agcaacggtt ttttcaagca ggatttcaaa
    14821 ccccatttgc agaaatgata cccatggagg tgtctttttt tgcgcagtaa aatcaaagcg
    14881 atgaacggcg aatcaaatct caggattatc gaatggctta aggcagaact gatcgaatcg
    14941 accagcgtat tgttcaaatc attgctaagg tcaggcggtg aagcgaccgc cgatgccttg
    15001 tccacgattg tcattatatg ttatgtgttg ggcaggagag tggggatcaa ttaccagtcc
    15061 attgatatga acattatgca caagataaac actacggtca atgaagcatc cgagattgaa
    15121 caaatgtacg gagatttaac tgaactaaag aagtatttgg atcgcaagga gagcagaacg
    15181 aggtgattcg ttgccggccg tattgaaacc atttttcatc acatttgcag cctgtctggc
    15241 tattcatctg tggcctgcgg gcctgttttt ctgggcagtg atctggggta cagccatact
    15301 gctggctgga gcatatatgg ggatgattca gttgctggca ctggcgggca gcaatttttt
    15361 attcctgctg ctgctgggag acagtgccct gactcttgac ctgttggtat tctatgccat
    15421 cccggccctg gtagcggcaa tcctgttaaa aaagcaaaaa acctattaca ccatactggg
    15481 aaaagcactg ctggcggcag tgatgagtgt gctcatctat atgggtataa gctatgcgta
    15541 tctgggtacg gagggaatca gccaaatcaa atccgggata gatttggcgg cggagaacag
    15601 tcttgcctgg gcggatcagt caggatttat ggaacgttat gagcaacggg gtatcagccg
    15661 cgaagagata aaacaggcct ataccgaact ggcgcacctg ttgctggttt ttacaccggc
    15721 tttgttatgc atacaggctt tgttggcgac cttcctgatc ctgtatagca gttcgcgaat
    15781 gtatcgtaaa aaacggttgt ccgggttgga gcgaaagccg tttcacgaag aaatcatgcc
    15841 ctggcagtta acctgggtgg tgattgccgg tctggcctgc tggctcctgg ggcgtgagca
    15901 gatgaactgg ctctattaca cgggaaccaa cattctggcc atcatgtttc cgatcacctt
    15961 cttttttggt tgtgcagtga ttgtttattg gctgcaacac ctgccagcca aaagaaaaac
    16021 atgggtcgtg accctggtcg ttttactggg cctgttcttt tccgtaccaa ccattatttt
    16081 cattggccta acgggcctgt ttgattcatt attagactat agaaaagttc gcagaaaaaa
    16141 ggaggggatc caatgaaagt cattctgacc caggatgtaa agaaacaggg tgtcaaaggg
    16201 caaatcatcg aagtatcaga cggttatgcc cgcaattatc tcattcccca gggcctggcc
    16261 gaagaagcca gcaaaaccag gttaaaagaa atcgaagaga aaaaagtaca tgatgccaaa
    16321 caaaaggaca aggaaaaagc ccaggccgag cagatcaaag ccaaaataga cggacagacg
    16381 attgaattga aagccaaaac cggcagcaat gacaaactct ttggtgccat taccagtcag
    16441 gagattgctg atgctttgaa acaaaaattc ggggtcaaga tcgacaaaaa gaaaatcgaa
    16501 caggcggagc ccatcaaaca tctgggcgaa tacagcttta aaataaaaat ttatccggct
    16561 gtccaggcag aactgaaact tatcgtcacc gccgaataaa tccaggagga acccccattg
    16621 aagcagccaa tgttcaagct ttttaaacgc aaacgattac agcccgaacc aaccgccagt
    16681 ctgaactcca aaattgccat cctgatcagt caaacggaaa aactatttgg cagtcaggaa
    16741 ttcattatcc gcgccagtaa actcaatgcc gtagaattgc ttcaggcaga acgggaagaa
    16801 gaccagattt taggactaca taaaatactg catgaagatc cgacccttac cgcactgccg
    16861 gctgaaaaaa ccggggcgca attattgcag gaactggaag agctgatcgc agagcaatcc
    16921 gcccgacaaa tggtggcccg gcagatggag cagcgcgtgc aggaactgat ggaacaaaac
    16981 tacaatgaat acgtgcagga aatccgcaaa agagtcctca aggaagccaa caaaagccgg
    17041 gaaaatgccc gtacgctaaa gaagctgggg caactggagg ttatggagca aaacggtctg
    17101 aatgactcgg cattaaattt tctccggccg ggctccatgg atgaaatggt gggacaggaa
    17161 caggccgtca aggcattggt gtccaaattg aattcgccct ggccgcagca tattctgctt
    17221 tacggacccc ccggggtggg aaaaaccagt tgcgcacggc tggctctgga gatggccagg
    17281 agcaaccctc gaagcgtatt caaagccaat gcccccttta ttgaagtgga tggcagcact
    17341 ttgcgctggg acccgcgtga atcgaccaat cctttgctgg gctccgtcca cgatcccatt
    17401 tatcaaggcg cccggcggga actggccgaa gacggcatcc ccgaacccaa actggggctg
    17461 gttgccgaag cgcatggcgg catccttttc attgatgaaa ttggcgagat ggatcattat
    17521 ctgcagaata aactcctcaa agtcatggaa gataaacggg tttattttga atcatcctac
    17581 tacgatccgc aggatgaaag gatcccgcaa tatatcagga aaatgttcga cgaaggcgta
    17641 ccggcagatt ttttactgat cggtgctacc acccgcagtc gggaagagat tattccggct
    17701 tttcgttccc gctgcatgga aatatttttc gaacctcttt cggcagggca gatcgaccag
    17761 attgtgcacc attccgcagt caaactggga atacgaatgg agcatgaagt cagccagatt
    17821 atttgtgatt acagcagtga cggacgcacc gccaacaaaa ttctggtaga tgcctatgcc
    17881 ctggcccgct atgaagacga tcctgaaaca acgggcggga ttgaactgag gacaaggcat
    17941 gtatacgaag ctattcaaaa cagccgaatc acacccgcca ttgccagcaa agcgtatgca
    18001 ggagcagaag taggcaaaat atatggactg ggtatcagcg ggtaccgggg tactctgatc
    18061 gagctggagg cgattgcgtt tgccgcagaa aaaggcaatg gcagcatacg attcaatgat
    18121 actgccggca gcatgaccaa agactcggtg gcgaatgcag cctcggtttt ccggaaaata
    18181 catcatgccc agcttaagga ttatgatgtg catatcaatg tagtcggtgg cggacaggtg
    18241 gatggcccct cggcgggagc ggccatatat ctggctatca gcagtgcggt gctcaactgc
    18301 cctgtttgtc aggatgtggc cattagcggc gaaatatcca tacaaggccg ggtcaagccg
    18361 gtgggcggca tctgccagaa aattctcagc gctcgccagg cgggagtcag aaaagtgttg
    18421 ataccggcag aaaacattgc cgatatcccg gctggggtaa aggatattgc cattatcccg
    18481 attcgaatga ttgaagaagc gtatgaacat gtatttgctg ccgaatcgca agcccaggtc
    18541 tactgaccag gcttcaagaa aggcagcagg ccttgcggtt gcccatttgg ttgacaccag
    18601 ataggccatt tgctaagatg aataggttga cgaaggaaaa agcaaccagt atggataaaa
    18661 tggttgctga ccaacgagat aaaccagggg ggtggaccta tgtcagcggt agtattagta
    18721 ggttcccagt ggggcgacga aggaaaagga aagataaccg attatctggc cgagaaggca
    18781 gattgtgtag tccgttacca gggcggcagc aacgctggac ataccgtcga agtggacagc
    18841 gagaaattca tgttgcacct gatcccttcc gggattctat accccaaaac cgcctgtatc
    18901 atcggcaacg gcgtagtggt tgacatgggt gaagtactga aagaactgca aggactgcag
    18961 gagcgcggaa ttgacaccaa caatctgcgc atcagcagtc gtgcccaagt ggtcatgccg
    19021 taccacaagg aaatcgatgc cattgaagac cgctgcaaaa agatcggcac gaccaaacgc
    19081 ggcatcggac ctgcttatga agacaagatc agccggaccg gatttcgcat ctgcgatttg
    19141 tatgatcccg gctttaaaga aagatttatc caggaaatgg agcgcaaaaa cctcctcctg
    19201 caaaaaatat acgatgcgcc cggctttgcc gttgagacca tctatgccga gttgatggag
    19261 caggcggcac agcttaaaaa gtatgtcgct gatacctccc tgttggtcaa tcaagtcatc
    19321 aaggacggga aaaaagtttt gtttgaagga gcccagggga cgttactcga catcgaccac
    19381 ggcacctatc cctatgtaac ctcctcctat ccaacttccg gaggagcctg catcggggcg
    19441 ggcgtcggac cgacccatat caatcgggtc attggcgtag tcaaggcata taccaccaga
    19501 gtcggcgaag gaccctttcc caccgagctg aacgatgaca acggagagat tctgcgcacc
    19561 cggggagccg agtttggcac tactaccggg agacctcgca gatgcggctg gctggatgca
    19621 gtgattttgc gttatgcaac cagaatcaac ggcttgaccg atttggccat taccaaactg
    19681 gatgtgctgg acagctttgc aaccataaag atttgtgtgg cctatcggta ccaaggagaa
    19741 ctgctctacg agtttccaga taacctcagc atcctggaag aatgcgttcc cgagtatatc
    19801 gaaatgcccg gctggcagga ggatataacc aagattgaac agtatgaaga cttgccttca
    19861 gcggcacgtg actatatcgc caaaatcgag gaactgagcg gggttagaca ggcgattgtg
    19921 gccgtaggac caaaaagaag ccagaccatc gtcagccatc agctatttta agatttgaat
    19981 ccttatggtc agggtgttaa aataaaaaca acctgaaaaa acgggcgcgt aactcagcgg
    20041 gagagtgcca ccttcacacg gtggaagtcg tgggttcgaa accctccgcg cccaccatat
    20101 gaatacagta atagcgggac tttgaaggtc ccgctatttt ttgttggact tatttcttta
    20161 gtgccatggc tttcatggct ttgcggtcgg ctttgccggc cagggtgcgc ggcaagtctt
    20221 gcaggaattc cacttcggag ggaactttat aaggggccag gttttcacgg caacgcgcaa
    20281 tcacttcttc agcggtcagc gtcgcccctt ctttgaggac aatgaatacc ttgggggttt
    20341 ctccgcgttt gggatccgga atccctaccg tgcaggcatc caggacctca ggcatggcat
    20401 aacagacttc gtcgatatca cggggaaaga cgttaaaacc gctgcagatg atcatatctt
    20461 tcttgcggtc aacaatgaaa aagaagccgt cttcatccat gcgggcgatg tctccggtat
    20521 aaagccatcc gtcgcgaatc gtcagggcag tttcttcggg gttgttccag tattcccgca
    20581 tcatctgagg cccacgccag atgagctcac ccggttcgcc caccggcatg atggtggtgc
    20641 cgtctttgtc atccacgatc agaacatcgg tatcgggata cggtacgccc acactgccta
    20701 ccttgcgggt atgatggccg gaattggcgc acacctgctg acagctctcc gacatgccga
    20761 agccttctac gatacggcaa tgggcttgtt cttcaaagct cttcatcact tccaccggca
    20821 gaggggagcc cccactgaat acgccccctt tgaagcagga aatatcagct tctttaaagc
    20881 ggggatggtt gagcatggcg atgatcatcg ccggtacggt ggggaaagtg gcgggctgat
    20941 atttttcaat cgcatccagt aatgtatccg gattgggtac cggcacagtg accagactgc
    21001 cgccgcagaa gaagttaaaa ccaacattag ccataaagcc gaagatatga ttcatgggga
    21061 tgcaggccag cgcacgtaaa tcgcccccgg gcatggtggt gtaattattg gtgacccact
    21121 gcccgacgcg cactgccttg gacatcacat tgaaattggt caggacgcag cccttgggga
    21181 caccggtggt tccgccggtg tattgcagac ggacaatgtc gtccatggtg atttccacat
    21241 ccggttctgc attgctggcc gcgccgatga tttcattata gtcatagata ttgtcagcct
    21301 tttcgatctc caccggcatg gtgggcacct ggaagacgat gacccgtttc accggggaat
    21361 tgggttcctt catgatttcg atggccttgt tggcgaacat cgccatcacg ataacggttt
    21421 ccgcaccgct gtccctgaac tggtaggaaa gctccgggac tgtatagagc gggttggatg
    21481 ggacttcaat ggctccgatc tttaggagcg cctgaatcga taaaacatac tggggcagat
    21541 tgggtgacat gatcgccaca cgatctcctt ttttcacccc caggttggtc agcgcattgg
    21601 ccaggcggcg gctcaaatca ttggcgagtc cataggttat tacgacatct ccttgcatga
    21661 cgtaaggttt gtccggctgt tcctctgccc atttattaaa ccaccacttg ggcggttcgg
    21721 tggggtactc cggatgcgct tcaattttgt catcgtagtt ggcgtgccac acgggtcgcc
    21781 atttggcaac ttcctcttca aacgctttta accatggctt ttctgacatt acaaacccct
    21841 ccttaaaaat agtgctgata ctttgttgcc gcagagatac ctctttcggc tacggatttt
    21901 gaatctaggt atcagccaga ctgtatactt cctccagcaa aactttcgtt caaaacctga
    21961 aaacaggaga tcactaacgg cttaataaac aacatcagga tttggacaat ggtttgggaa
    22021 cctactccaa tgactatgca ataataatgc catgccgtaa taaatcgcca taggaaggaa
    22081 gttatatcac cattggggca tcaagaagca cgctaaatga agcaattttc agaacaaatc
    22141 ggggtctttt gatgcaatat tgtatcgatt ggataaatat aaaattattt atctgcttca
    22201 atctggttta attggaaagc acaaaaaaaa aacaaatgtc tgccagattg atacaaaaat
    22261 gcatcgtttg ttgcattttt ggaaacggtg gcattgttcc gctgtttttt caattgttag
    22321 tggagtgttc atgcggagac acaaaaaacc tgccttgcat attatggtaa tggaaactat
    22381 gttagagatt caggccgagg caggaaaggg gtgttttaaa tgactgtaaa tataaactgg
    22441 aaaccaagcc cgaactatac tgctggacgg ggcgggagaa aaattattgc catcgtcaat
    22501 catcagaccg gaggacaggg accaggagca ttgagctggc tgagcaaccc ggcatccaaa
    22561 gtatctgctc actatttaat tttccgggat ggaacagttt atcaattggt taggcttcag
    22621 gacacggcct ggcaggccgg ggctgtcgac cagccttcct ggatgttata tgacggtacc
    22681 aatcccaatc gctataccct tggaattgaa catgaatgct atcctgcagt gggaggagac
    22741 ggaaatctta ctgaaaggca ataccacgct acgcttgaat tgcatcgcca gttgatcagc
    22801 aatcattcta ttgtggcgga tcgcacgcac atcatcgggc attaccagct cgacagcgtc
    22861 aaccgttcta actgtccggg aaggaatttt ccctgggaac gtcttatgca agatctaacc
    22921 ctcggaacca cttccgtacc gatcgtaacc agccccaaaa agctgatggg atttgtgatc
    22981 aacggggtat cctatgcacc ggtccgggcg ctggccaacc aactgggcag aacggtggaa
    23041 tgggatgcgc ttcgcaacgc tgtcctgatt cccccggtta cggtggctgt gccaacagcg
    23101 caggcggaca aagtttttgt ggttaccggc gccgttgtta tagagggctt gctgttgggc
    23161 ggaacttctt acgcaccggc gcggactctg gcggaggcac ttggctacca agcggaatgg
    23221 gatgatgcca acaaaacggt aattatatta tagccaaagg agggtgtcag cgggacgttt
    23281 ttcgttccgc tgttttattg tctggacacg ccgagacacc gcaggatgaa aatgcatatt
    23341 atggtaaaga ggagatagga ttgcggccgg ctatgataat ggtgagtaga aaagaaaaga
    23401 tcacgcatcc ggacggctgc aaccacccgg atgcgttaca tatcaattga acagggattt
    23461 gatttccgta tcaaagttct gaatgacgag attgcgtttt actttgagag tgggggtgat
    23521 ttcacctgat tcttccgtca ttctgcgggg aagaatagca tactttttaa tcttttcata
    23581 cgattccagt tcattgttga tgcgttggac ttctttttca accgtttcct tcagcaatgg
    23641 ctgattgatg aaatcatcgc cgacactgga caccgtcttg acgccactga catcgatgta
    23701 tttaatcgcg ttttgatcat aggacacccc gtttttctca aataaagcca tgaattcttc
    23761 aaaattggga acaatcaagg ccccaatata tttctggctg tcacccaccg caaaagccag
    23821 atcaacatac gtactggtgg aaaaggcctg ttctaccttg gaggaggcga tattcttacc
    23881 ggtatccagc acaatcaagc cttttttgcg atccacgatt ttaataaagc cttccggcaa
    23941 catctccaca atatcgccgg tcatgtaata cccgtcctcg gtaaacgcat tgcgggtggc
    24001 ttcggggttg ttccaatact ccaggaaaat attgttgccg cggacgcacc attcgccgtc
    24061 ttcggccata taaccctcta cacccgggca gagtgcgccg accgaacctg gcagtacacg
    24121 atgcacccgg ttcatgttga tggtgttgca ggtttcggtg gaaccgtagc cttcatagat
    24181 gggcaggccc atggccagga acagcttgca taagtcagcc gacaggctgc cggcagccga
    24241 acaagcgaag acaaaccggc cccccatcat ttcttttact ttattgaaca ccagcttgtt
    24301 gtataaacga tattttaatt taagaaagaa gctggcgttg cgggtgatgt ccatatgggg
    24361 gtagacatcg ataaagccgt tttcatcggt gatggtttgc accacttgca ggccggtatc
    24421 aatggccttg ttaaagatgg tctttttaac tgctgactgg gaggtggaag cccgcagggc
    24481 catatagatt ctttcgtaga tgcggggaac ggacatgaac acatgcggtc ggaaagtagc
    24541 aaaatcggcc actagtgtct ggggtgagga gtaggctacg gcgatggccg tctgcatcgc
    24601 cagcgcatgc ccgcattccc gttcataagt atgagagatg ggcaggaaag agagcagtaa
    24661 gtaatcatct tcgataaagg gagagaaggt cagatcacgc aaaaccgcag cattgatgct
    24721 gaaatgggta tggacggcac ctttgggatt gccggtggta cccgaggtat agacgatggt
    24781 catgcgatca tgcagtttga cggtgcgcca tcttttttca aaagcaaaac gatcctgcag
    24841 caacagaaca ttgcccatct ctcgtaattt attcagactc atggcctgat cgctgataaa
    24901 gtcggcgtca cggtccatga ttataagctt ttccagggag gggatattcg cagaggccgc
    24961 caggcatttt tccagatcgg cctgcttttc aataaaaagg attttggaac cggaatcgtt
    25021 catgatgtag gtcatctcgt tggcactgag ggtgggatag atacagacgg tgatcccagc
    25081 tgcgcagagg atgccgtaat cacaccagac ccactccggg cgggtattgc tcatgatggc
    25141 tacccggtcg ccgggctcga cgcccaaggc catcaagccg caggtaactt ctttcaccac
    25201 ttggccaagc tgggcgtagc gcaacgattc aacttcacct ttctggtttt tccacatttg
    25261 cgccgttttc gctgcgaatt gggtcacatt gcggttgagc aattcaggga aggtctgttg
    25321 ggacatgtac catcggtaag agggttgttc catgtaatcc ataatatgcc tcctccaaaa
    25381 aaatattacg ttacgtaaat tatacaccaa acttataaga aatttagata aaatgtcatg
    25441 tttattacaa tgaacgcttt ccgtcaagtt acgcaaaaga aggaaaacag ctgaaaatcg
    25501 agaaaacact aagtaaatat tgtatagggg ctgaggaaag gggaaaaaaa gtgttgatta
    25561 catattttgg caaaagttgt cgagccggca tgaagttggc tgcttggctg gttatgataa
    25621 tgttgctgat caatacctgt tctttgactg cctgggcaga tattaataca accaaagaaa
    25681 attctctgaa ttctaatcaa aatattcttc aatcgctgca gacagttcaa ggagaggcaa
    25741 caacgcctga ggctgcccga caggtgctgg aagagttgga tttatgggac gacagtgaca
    25801 gccaacaaga tctagcgaca gtgataaatt ggcgtggtca ggcaatgacc ttggacgaga
    25861 tctggaaact gatcaacagc ccggaggtaa atcttgatga agccgccacg gtagatggga
    25921 cttcaatcac cttgcgtgat ctcaaaacca tgattgccat tgaatatgaa attcaacgca
    25981 tcaaaaatac ctattttaat gatgtaaatc tggataaaga gcatcagcaa accctgtctt
    26041 ccttgcatca gcagattgcg acggagggaa ttaaaattta tccacagggg attgtgccga
    26101 tggcaacaga atcctcttcc ctggtttttc cctccggcat tgaccagggc atctatgtcg
    26161 aagtcgatgc cagccccctg atctatgaca ataacagcgg tgagcagacc atcacagcaa
    26221 gactcaagga tcgtcaggga aatgcattga cgcaggtgcc tgattatgac atcagctttg
    26281 attatcgact gctgcaaggc agcgccgaat ttgcctatga attcactggc aaccgcaccg
    26341 gaaccgttac atttcccgcc aagagcacag caatcaccca aaaaataact ttgcgaatta
    26401 acgattccat cgcctggcgc caccttccct ttgaaggcca gcgcagcttt ctggtgcaat
    26461 tttacaaccc gcacaacgtt acctttcagg aaggcaagcg attgatcgag aaggtcataa
    26521 aaattaacag caattatacc tttttgccca tcaatgaccc ttttctaaat gacagtctac
    26581 ccggctacct tgctttggat tacggctcca cggtatcctt tgcctacgac cgcaatgtcc
    26641 tcaaaaacga attgttcacc caaaaagtgt ttaatgaaat cgaggttcaa ctgtccgggg
    26701 ggacgccatt tgtcggttca cccggcaata cctccgctta tgaattccgg cccgccattt
    26761 ttggaaaata ttttggcgac gtgctaacca tcaaatcagt caacagcata attgatcatt
    26821 tcggaaccat tgcgctggat gatgatgtct atgatgaatt gatttacgac aacgataacc
    26881 agctagatgt ttacttcggg cacaatcata ccaatccctg ggatcattgc aatgtaaccg
    26941 ccaaagtggg attatcgctt cggtttatcg atacaaagaa tccctgcgtc aaggattttt
    27001 tccggatcga cagcaagccg tgggatgtag gcaagacctt tgcttacgga caatcgatcc
    27061 cgatttgcgt gacttattcc gagccggttg atgtgtccga agctaccctg caagccaacg
    27121 ggatgaccct tacgccgatt gaagctggca gccatggagt gacaagtgca accttccttt
    27181 acccggtcaa acagattgat gatcctgatc tgcgcatttc cgaaccccgg ggtgccaaag
    27241 atttggccgg aaacctgcaa gtaccttcgt ctgacagtgt gatggaaagt gttaccttca
    27301 agaacatcat tcagcctcag tacaagctgg atacgtttaa aaaagcatcg gccagcctgg
    27361 tgactgcaga aaacggagtt tcagtttgtc aggtagaaat tgatttaaat gataaccagg
    27421 atgtgaccaa ctggctggtc agcgaggccg gtgtcaatta cgatgaaacc gttaataaat
    27481 accgggttcc cagcatttac gcctccctcg acggaggggc cacaaaaatt gacctttatg
    27541 ccgacaatga tataaccccc accaaactgg tgggcagttt tgtgccagcg ttcaatgaca
    27601 gtgccgtgac ccggtccggc gtggtcgaat tctatcttga tccggtcatc cacagcagta
    27661 ccaaccatca gctgctggtc ggcacctatg ctgtctatac cgtagagccg gttgtttttc
    27721 tgaccgatga tgatttgacc atcagcgaca actttccggc cgatggaaat ctttttgcag
    27781 atcaggcaaa cgtctggaat ttaaactaca gcgttgccaa tacagcggct acttatcagg
    27841 gggcagatga tttcgtctgg tccagcagtg atgaagccat tgccggaatt acaaatgacg
    27901 gcaggattac gctgaccggt actccgggca gcgtaacctt tacccttacc gctttaaacg
    27961 gacaggcagc tggcaaacag gtctcgctga acagtcgcac ggttatcgtc aaagcaggtc
    28021 tgaccccttt cctggtgatt cctgagcaga tgaatgccat aaccatcaag cagggagaag
    28081 atgtccaggt gcgctggaca tccaacctga ccggcaaaaa tgctgaggca ggcggaagta
    28141 cggtttttga tgtggacgtc agtatggctg attatcgtgg aggatcgttg cgaaaaggcg
    28201 attccgtcta ccgcagcacc gttacttcca cccaggaaaa ccccgtttcc ggatgtacga
    28261 ttccagctgc ctatgttggc aatatttccg cgatgggcag atacagttat atcatcacga
    28321 tcaccgcacc ccatccctat gatgctgccc agaccttaac ggcagctgct tacctgtcag
    28381 tggcggccaa acctgccgtg gtccgactgg gaaaactgga caaatacttt attagcgacc
    28441 gcacccccag cgttcccatc agttggacgc tggaaaactt cgacgaggtc aatgcagcgg
    28501 aatttgtcct ggaaataagc aaaaatgacg gaaagacacc gttctacacc cagaccggca
    28561 cagactccag cggaggctcg tatgatctgg cgatccaaac ggtagacggc ggatttaaag
    28621 atctttatac ggtagctgta aaagtcagga ataaagggga ttcagcctgg tcgtatgatt
    28681 cttttgtgtt gcatgtgtac agccaggatg cattgcatat atggattgac ggggcagctc
    28741 cgggaaccac ttttaccatg agcaaccttc ccgccataag tgccatgagc agcgaggaaa
    28801 tcctggcgct caaacgcgat ataaagctgc gcaacgaggc ttccataaac ttcggtgacc
    28861 atggttgggg gcagatcagc gaccagatcc agtggcattc cagcgatagc cgcatcggca
    28921 gcctgaatta ccaacagggt ggctattatc ggaacattga agaattgatt tattcttcat
    28981 accggccgga aacaaaattt gccctgtccg ggctgcatga cggcaataca cggctaacgg
    29041 taacccacgt tgccaccggt ttgcaaagaa ccttggatgt ggcggtggaa acgctgcagg
    29101 acaagcttta tttgttccaa atcctgccgc aggccgaaac tactttgaca tacgtcaacg
    29161 gcaacggaga acaacgcgtc gttacgacca atgccagagg agaactggcg ctgtatgaag
    29221 caagcggtat cgccggtgaa atttatctgg agtctgctgt cgaagaacgg atctatatgg
    29281 ggaccttgta caaaggactg ctttcttccg aaaaggactc aacccaaatg gcaatgtatc
    29341 cgctgaatct atttaaactg cgggaatgtg caacggtaga gctgtatgta agaaaaccgg
    29401 atggaacccc ctatcacgga gaagtattct tgaatggcgg agtttacaaa aatgaccgtt
    29461 attgtccgac tgcgctgttg aacaaattgc cgggagatca atcccaaacc atcacgacag
    29521 accaggacgg caagatcatc gtgcgcatga acaccagtca attttggcat gaatccagcg
    29581 acgaggtatt aaccggagcg gataaactca attttattta tttgttgcat tttccgaacg
    29641 atgagtatta tccgcagatc atcaatctgg acggcaatat gagcagtgaa gaagtagtga
    29701 tgtctggtgc aggcattgtt tctctggaaa tggtgggcga aggggagaaa tacaaaccat
    29761 ttatcgctgc tcagacgatt acatatgaaa cggaaagaga tcgccgcatt gatgtacgca
    29821 aattcaaagg aaaaatcggt cccagtgaca cccatgacaa ggtcatattg aatacaacgg
    29881 tgttgtggtg gggagacagc aaagatgacc cgtctgagga tcgccagttg tattttcaag
    29941 acaggtatgg aatggcattc aacaatcaga cccatacaac ggatgaatat ccatttaccg
    30001 acatggtcag ttcaaccaat acatttacgc ttgaccgggc tacggttaca gaatggagcg
    30061 agccggtcca ggacctgcat ttgatttaca gtcattccgg ctcgacccgt tacatcaatc
    30121 aagaaatgac ttttcgggcg gccaacatgc tggatgtacc caaagccatt gacagtgaca
    30181 gcctggtcat cactctgcag gccatgtcag ccaatgccaa ggccgaggcc ggcagcttga
    30241 gtttggcgga tgaaatgatc ggggccggat tatcacatct cagccagcag ggaacaggaa
    30301 gtctggggga atttttccag atggtcatcg cgcctactgc cgatccgact gtttatgttg
    30361 ctctgatcgc cctcaatttg ggggggctgg ccacggaaga cccccaaacc ggcagtgaac
    30421 tggaatatac ccctggtttt accgacgcca tggatatcat gaaaaagaaa tatatcaccg
    30481 aacaaaaggc aaccctggac aagaatcgtt ccggaaaaag cttttccgag aaagattttt
    30541 ctttcgtctt tggtgggtat atggaagccg aggtgtttta taattttgac cacggaaaat
    30601 gggaagtact tgttcttaat ggaggtttca acgccggtgc gggcctcgac tacacctgga
    30661 attacaatac catggtgggg cccattccgg taacggctca acttggggtc ggggccagtg
    30721 cggaagtgat tttcaaagcg gccgtacagc gaggagaaat catcaagaaa acctatgacc
    30781 aggatgccgt caatgactac ctgacgacgc tgcgtatcta tgcatatttc aaggcatttg
    30841 ccggagtcgg ctttgattac tcggtagtag ccttgaaaat aggcttgttt ggccgaattt
    30901 ccctggatgc gcaatttgcc tggctgaacc agccctatgc caaaaccaag aaaaacggac
    30961 aaagtctgac cgtcaacgga cagattggca tcgaggcgga tgccaaattc ctgtttgtat
    31021 cggaaaccta tatattgacc tccagaaact ttaaaattgc cggagcaacc tataacgatt
    31081 gggaaaaaat ccagactacc tgggaaaaga tcggcaaggg ctccaatgac aatctcgatc
    31141 ggctggcgat tcccaatgct atgatgccgg aggatttgag cctgtatccc atctccgctc
    31201 aatcaaccct ggaagatcga gattatttgc agcaattcca gcgttcctgg gcgtcttcac
    31261 aggaatttgt acagcaatca ctggatgagg aaaacggcgt ggatagtttg cagaccaata
    31321 cgtatccctt tgccaatccg ctgttctctg atgacggtca gatactggtc tacgtttacg
    31381 acggggacag tcaagagatc gaaaacaccc gggtatactg gagcagcctc caagatggcc
    31441 aatatccgca aggagttccg cttgcggcgg agatgaccag cttcggcgac tcccagctgc
    31501 aactggcggg cgatgacgat tttgcggctg cgacgtgggt cacccagagc gaaaccattc
    31561 cccgggaagc agggcaggaa atcagcaacg ccgacatcgc tttgttgagc aacggcaccg
    31621 aagtagtggc gtcagtttac gaaaaccgtc aatgggtgac gacccggctc agccagaaca
    31681 gtaccccgga tatggcgcca acagtagcgg taaatgacag ccgggtgctg gttgcctggc
    31741 gcagcgtgta tgcggcagat gccgaccatc ctttggaatt caacggtgcc gataatatcc
    31801 tttaccggat gtatgatcgg gaaaaaaaga cgtggagtga gaccaagacg ctgtacaacg
    31861 gcagcagcgg cgcggtgaaa gcccttgaag ctgccatgct ggaggacagt acggcagcgc
    31921 tgacctattc cttggatacg tctgcagata cggcaggaag tgaccatctg ctcactgttt
    31981 ccgatgatgg caaaaaagtt agcagcggca tggaaatcat ctatgcagtc attgaaccgg
    32041 aaggagcatt ggggaaaaac gtacgcgtga ccaacgatgc ctttttggat gaaaaccccc
    32101 agctgaccgc cgtcactttt gatgatgata tcgagcgctt cgtacttggc tggtacagca
    32161 ttcatgatgc tgatggagtc agccgcaacg atatccgcct gtgtgccttt gaccgctatg
    32221 gctcgattta tccggatttt attgacagta tcagcagcgt taacaaccat tccaatgtga
    32281 atatcagttc ccgttttaaa ttcagcaaga atgccccaac aattgatcaa ctgtctattt
    32341 tatggaaaga gccggcgaat actgcaacca ccgaaggaga tactgtcaaa gcagacaaag
    32401 attcgctgaa ggcggtcaag ttcattaaag aaaacggaaa aatatttatc acctcggcct
    32461 tggatgtagc aacgatgagc gattttacct tgatcgatca ttttgatgcc cggtgtgtgg
    32521 atcaggatac ggtcaaggca gtaattctgg gcaccaatta cgataatggt taccgggaac
    32581 ataaaatccc tatagaagta aacggagaga gaaccgagca gatcatttat cttccggtgg
    32641 cccgctctga tctgtatacg gctactgcag tctatgaaaa taaaatgtcg gtcaatcagg
    32701 tagcggtaga ctatcgtacc atccaaccgg gcttacggat accggttcag tttaccctgt
    32761 tcaatgcggg cgtacagcca attgataata taaccatcca aatcgggaat aatatcaccg
    32821 agtttaatca gggactgttt ttgttgccca atgaaagcat tacactaacc gccacttatg
    32881 atattccgac cgctcaggtc atcaatccgg actacaacat cacggcccat ttcagcagca
    32941 tgatgccgac cttgggcaag caatatacga ttgccgacac cttgtatctg gatgtaccgg
    33001 atgtaggcat agccaacaca aaaattgtcg gggaagcaaa cggccagagg gttgtccagg
    33061 tgacgctgta caacaatgga gcagccttgg caggaagctc gcgcaatgtc aaaataggac
    33121 tgttcagcga cccggaatgt acccatctgc tgcagatggt aaacggtcag gaagagggag
    33181 ttcccttcag cgtagatccg gatgattttg ccctgattga tgccggcgct tatacccgac
    33241 aatttatctt tgatattgcc ggacatgtga tgctgggcaa tgagattccg gaaaacggcg
    33301 tgcagatctt tgtcaaggcc tgggcggaag aaacgcttct taccggtgac acagcggcta
    33361 atctggaact gatcgaatac tatcgccata ataatatttc tacagtactt tttgaaagtc
    33421 tgctggttcg caatgattat cagccggtga ccttgtctac ccaactgcac tgtgaagagg
    33481 ggtcttccac ggtgctggtc gatttaaaga acaatgccct ggccagccgt catagcggca
    33541 atctgatcgt cagcctgttg gataaaaacg gccgcatcat tgacactaaa cgcagctata
    33601 accgttttct gttgggagcc ggtctcctca atctggccgg agaagaatcc tcccagtcag
    33661 tctttacctt tgaccgtgag ggagcagcgg taatggttac ctacgccgat gtgaacaaag
    33721 aggaagagga aaataccggc ctggcatctc atttcatgca agggatacca ctgcagttgg
    33781 tggaggggca gaaggagtat tattatgaaa cggaaaatct gaccgccact ttcatcaata
    33841 tcgcagcgca agatccggat gctgtcatca aagtcaatgg gcggccgact gatttaggca
    33901 gcgcggtggt cgatctgcat cacggtatca atacgattac cataactgta acttcagctg
    33961 atggtacagt caccgataac tatacggtaa cgatcagcaa cagtctgcag acaccccccc
    34021 aggagagcgg ttctcattcc ggatcgggta cagctacgga tgccaaaggc atcagtatca
    34081 cttcggaagt aaaacaagga gttcgcttat ttagtatcaa aactgacaat caagtgttgt
    34141 ctgccgcggg cggaagagtc aacgttgtgg tgccggctga aaacgccagc ccggaaacgg
    34201 tagcgatcag ggtttatccc gacggacgcg aggagattat cccgatggcc ttgcttggca
    34261 acggacagat gatcatttct gccagcgaac cgatgactat aaaattgcag gaaaataagc
    34321 cctcctttaa tgacattcaa catcactggg gcagggatga tatcctcttt gtgggggcca
    34381 gaaaactgtt tgccggcatc gatcatgaca aatttggtcc ggacatgacc atgaccagag
    34441 gcatgctggt aacggttctg ggacggcttg gtaatgtacc ttccgaaagg tacaaagggt
    34501 ctgctttcca ggatgtaagt ccggatagct attatgctcc ttatattgat tgggcacaac
    34561 agacaggcat cgtgaagggc accggtgata accggtttgc cccggatgag gcgataacgc
    34621 gggaacagtt ggcggtcatg ctggccaatt acgttgcttt ttcccagctt ggcctgaaaa
    34681 atccgataga caagaagccg tttgtggatg atgaaaagat cagcgattat gcccgggatg
    34741 ccatagaatc aatgcatcag gccgggatca taaacggccg cgacgatcag cgtattgatc
    34801 cgcaaagtac cgcttcccgc gctgaagtag ccaccatgct ggccagattg ataagaaatg
    34861 ttatgcaaca atagatgcgt tcgccgattg cgtaaactta aggaggtaac gagtgagcat
    34921 agggtacgct tgtctgacca ttggggtgca gaatactgat ctgaaaactt gtatgctgaa
    34981 aaacgtcaat gagagcaggc tcgctgaact aatcagccat aatctgcagt ctctggaaag
    35041 aatcatcgat tataacctgg ccaaccgcat caggcttttc agaatcagct ctgatctgat
    35101 accgttcggc tccagcccag ccaatacact ggtctggcca gagatattta cagcccggct
    35161 gcaaaccatt ggagccaaaa ttaatgacag cggcatgcgg gtttccatgc acccggggca
    35221 gtatacggta ttgaattcgc ccgatgcagg cgtagttgcc cgggcaatag aggatctggc
    35281 ctatcacgcg caagtgctcg aaagtttggg aaccggttcg gaacataaaa ttgtcctgca
    35341 cattggcggc gtgtacaacg acaagaaaca ggcgatcaag cgatttattg cccattaccg
    35401 tcaattggat tcggcgatta cacgtagatt ggtactggag aatgatgagc gctcctacaa
    35461 tattgaggaa gtgttggaaa tcggcacgaa actgggggca cctgttattt ttgacaatct
    35521 tcatcatgcc atccatcctc ctgccggtga aaaagaccag ttctattggc ttgagcaatg
    35581 ccggcctaca tggcatcccc gggacggaca gcaaaaaatc cattattccc agcagaatcc
    35641 ggggaagaag cccggttccc actcggatac cattcatatc aatgaatttc tgcgctttta
    35701 tcacaccctg aacagaaatg atctggatat catgctggag gtgaaggaca agaatctttc
    35761 tgccctgaag tgcataaact gtaccttggc ggagaaaaag atcaagcagc tggaacaaga
    35821 gtggagcacg tacaaatata ctgttttgga acggtcacca tcggcttacg cccagatccg
    35881 gcagttgctg cgcgataaaa acaattatcc ggccgcagcg ttttattccc ttttggaaga
    35941 tgctctgcaa agtccgctgg agaccggtca cgccattaat gctgcccagc atgtctgggg
    36001 atatttcaaa gatgtggcca gtgaaaaaga aaaggccagc ttttttcaaa tcctcaatga
    36061 ttatcagcag ggtaaaacag ggttgccgaa aacaaaatcc tttttgaaaa aaatggcgga
    36121 caagtatcag cgccgttatt tgttggacag catgtatttt attctttagg aaataaaatg
    36181 ggatatagtg atttcaagag atattgaaag aagatgaaag tttattcagg gagggaagct
    36241 taatggtcaa tcaaaaagaa gtcacatcca taaaaatgct ggttttagtt acgtatctgg
    36301 caatgatcat cgtcaatgcc ctggccaata tcctgcccat caacggcgtc acgaccgggc
    36361 aggtttccga ttcctatccc aatctattcg ctccggccgg gagtaccttt gccatctggg
    36421 gactgattta tcttttactg gccttgtatt gtctgtatca gatgggtctg ttccgggatc
    36481 cgcaagcccc ccgcaatcaa gctttgctgg ccaggaccgg actgattttc agtatctctt
    36541 ccctggccaa tgtggcttgg attttttcct ggcacaatta catgatcgct ttttccatgt
    36601 tatggatgat cgtcattttg ctgtgtttga taaccatcct gcagacgatt cgccaggaaa
    36661 cgcttacccg gcgggagaag ttcctgatca aacttccttt cagtgtctat tttggctgga
    36721 tcaccgtagc caccatcgcc aatgccacca ccctgttggt tagctggcaa tggaacggtt
    36781 gggggattgc tgaaccggtt tgggccatta tcatcatcat cgttggtttg ttgattgggg
    36841 cggccaccac cattataaac cgcgatatcg cctacggtct ggtaatcgtt tgggcatata
    36901 tcggcatcta tatcaaacat acttctttgg ccggattttc cgggcaatat ccggctatca
    36961 tcaacacggt gctcgcatgt atcggaggaa tgctggccgt gctggtctat gtttacatcc
    37021 aggtcaaacg gccgatgcat tgattattct tttattgcag gaggagcaaa aaagaacgcc
    37081 cccgcgggga cgttcttgct tttagagggg aatttaagtt attcaatttt gttggttctg
    37141 cgccagattt tcatttcatc ggcggccttg atcagttcat cccggaactg gggatgggcc
    37201 aggcgaatca acccttccgc cctttcgtag gtggatttgc ctttaaactg ctcgattccg
    37261 tactccgtgg ctacatactg caccagggag cggggcaggg ttacgatggt tcccggggcc
    37321 agagtcggca tgatccggga atgcaggttg ccttccttgt ctgtataagt tgagtctata
    37381 cagataattc cctttccgcc gtgggaatta aaggcgccca tgatgaaatc aagctgtccg
    37441 ccggtaccgg atacctgtcg ggtttgaacg ctttctgaag ccacctgggt aaacaagtct
    37501 atggcaaggg cgttgttaat ggcgacaacc ttatcattca gcgcgatgac ccggggatca
    37561 ttggtatagt tgactggata aatggcgcat accggattgt tatccaaaaa gtcatacagc
    37621 ttctgggtgc ccagggcaaa ggtgtagacc attttgttcc tgtcgatggt ctttttggca
    37681 ccggtgatca accctttgtc atagagatca acgcaggcgt ccatcatcat ttcactatga
    37741 atgcccaagt ctttgagatc actgtcagcg atcagggccc caacagcatt aggcagcccg
    37801 ccgatgccca gctgcaggca ggcaccgtct tcaatctcct tgatcagcaa attggcgatg
    37861 gtttgttcat tttccgtagg catggctggc gtcagttggc gcaacggaat attgttgcct
    37921 tccaccacat agtcgacttg ggaaatatgg attgattcct ggttgccccc cagacacacc
    37981 ggtacagtgt tgtttacttc cacgattttg atcttggttt tttccatgat cgccggagta
    38041 attgagttgg acggacccag attgaaatag ccgcgcgcat ccatgctgct gacgctgaaa
    38101 aaacccacgt cgatgttgta atacttttta atgatccggg acccctggtg atacgtattg
    38161 gggatataat tgcacagatt gtggtcatgc aatttacggg atacgatccc aaaatggaga
    38221 tccgtcagga taaaatgttc tcttttagga tcagccaaca ccacctgggg cggggaggta
    38281 taacataccc cggtgactcg gacatcctgc agttcattga cacgtttgga caaagcttca
    38341 tccaatgcca gcggaaacat ggtaaactcg ccataagcga cctgatcgcc ggatttgacc
    38401 atggcggcgg cctgttcagc ggtaatcttt ttctgttcgt attgccattt aataatatcg
    38461 gacatggttt cacgctcctt ttagtagtac attctgttat tgtattgata tctatacgaa
    38521 gagtttgatt tccgaacgcg ttaagcgatt accttaacat aatagtaata ttatttattc
    38581 taataatatc agcttgtgca aaatttaaca agctctctgg agcatatttt ataaacgtta
    38641 cctggcatta ccaatcgata gaaagaaggc gtctttgttg tttgggatta ctcatcatga
    38701 aatgtggttc atttatttgc tggctgtcaa caccgtctgt ctgctgcttt tctttattga
    38761 caagcgacgc gcccgggccg gccgatacag gataccggaa cgtacgctct ttctgtgggc
    38821 agtcatcgga ggggcagcag gaggctggct ggggatgtat ctgttccggc acaaaacccg
    38881 tcatggcaaa tttacgttcg gcttcccctt gctgttattg ctgcagggcg tattgattta
    38941 tttcataacc cggacgggaa tataaccgcc ggactccgca gacctataac atttgtcccg
    39001 cgcttgcttc atcatagtga attttatttt cgcaattctc ccgggcgttt cgtgatcaaa
    39061 cttatacctt gcgaaaacaa tctgattatg ataccatgtt tctgcatgcg gagccgtggt
    39121 gtagcggtta acatgccggc ctgtcaagcc ggagagcgtg ggttcaaatc ccatcggctt
    39181 cgccatttaa aaaaacttgt caacttgaca tatagtaatt attcaagcgg gagtagctca
    39241 gtggcagagc accggcttcc caagccgggt gtcgagggtt c
//
LOCUS       DAOTHZ010000010        37745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1178_length_37745_cov_7.023799, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000010 DAOTHZ010000000
VERSION     DAOTHZ010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 37745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37745
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_37745_cov_7.023799"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="P5549_02380"
     CDS             <1..510
                     /locus_tag="P5549_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HRW11952.1"
                     /translation="ILVKQDTAADEYARAVALADAIKAESPDLILAGHVASDDGSSQV
                     PTRIAEILGLPHVNVITSIEIADGKATCTSEADGGTAVTEVAIPAVISSQVSWNEPRY
                     PSMKGIMQAKKKPVATVDAVAVDNKVKIVEVGLPAGKTAGIKIEEDPDVAAAKLADWM
                     KNTVKVEVK"
     gene            545..1498
                     /locus_tag="P5549_02385"
     CDS             545..1498
                     /locus_tag="P5549_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRW11953.1"
                     /translation="MAGTWVFVDQRDGNIRKVSYELLSEAKKFGGEVAAVIFGQGVEG
                     LAGEFAKYGADKVYVADDAIFAAYNTGAYVAQIVAMIKEYSPNAVLFGHTINGRDMAS
                     RLAQKLDAGLATDAIAAEISDGKGIFTRAIYAGKAIAKVEVTGSPVVATIRAGVWEVV
                     EGAGAGEVVKAAIAATAADVYQAAKEFIPTVSARPELTEAAVVVSGGRGCKGPDGIKV
                     VEQLADLLGAALGGSRASIDSGWLGHELQVGQTGKVVCPGLYVACGISGAIQHLAGMS
                     SSKFIAAINTDTEAPIFGVADFGVVGDLFKVVPLLCDELKK"
     gene            1660..3789
                     /locus_tag="P5549_02390"
     CDS             1660..3789
                     /locus_tag="P5549_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRW11954.1"
                     /translation="MIFGFKPLEGGYDIPMREVGANIPFDWLIYIIMFIPIGIFLYGA
                     YKKYLMWTIGKGEIHRNDKVGARTVSFLVNSFLQARVIKKPLAGWMHFFLFWGFLFLF
                     MAAGIDAAHHWLGWPHLEGFLYMGMSWIVDVTGFLAMIGIMVLAYIRYIQKPDRLNDT
                     KASDGWIILLIFTILLTGYFIEGLRIAAQIQLSTTMAQIAYERVCSPFGWMFAALFGG
                     MHLDSILIWHRLLWWFHMAIAFLFIAIIPFTKLWHIFAGMVNYVFRDLEPSALRFVEN
                     IEEAETFGVERIEEFGWKDLLDLDACIRCGRCQENCPAYNTGKHLNPKIVIIQNMRQH
                     LEEKMPYLLEAKDNPEEVAEIAMTDAAAEKVSPMETSLIYDVVTTEAIWDCTNCRACM
                     EHCPMYIEHIPKIVEMRRNLVMWQGDMPGEAQMAFTNMERNYNPWGVGWAGRAGWLEE
                     RGVRDLVNLLPEDGKEFEYLLYAGCAVSFDDRYKKVGESLVRLLDKAGVSFGYLGTEE
                     FCCGDSARRLGNEYLYQTLVSQNLESFNNYGVKKIIVVCPHGYNALKNEYPQMGGNFE
                     VYHYTEILAKLVAEGKLKPAKSVGAKISYHDSCFLGRHNGVYDQPRNVLKAAGASIME
                     IDQSRNFGFCCGAGGGRMWMEEEAVEGFKRINDTRTDQLLVTNPQMIVTNCPFCLTMI
                     SDGVKAAEAEENTKVFDVAEVLWQSMQ"
     gene            4138..4920
                     /locus_tag="P5549_02395"
     CDS             4138..4920
                     /locus_tag="P5549_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW11955.1"
                     /translation="MANYQHVLVGKEGKIASLTVNRAEKFNTFGPDVWREILLAGQEI
                     MEMDDLGAIVISAKGDHFSAGIDLKDLNTFNSEYVFKSIPWAQSVYSFWEEIPVPVVV
                     AIQGLCYGSAVELILACDIRVAASNARFSIPEVRFGLAPDMGGTTRMTKLVGSGQAKR
                     MIMCCDEIDAAEAKSIGLVEIVAEPENLMQTAMKYANKLTSLPPVAVKMAKKGINLAV
                     DSSVRGGLLFEQAQTTYCCGTEDQKEAIAAFYEKRQPVFKGR"
     gene            complement(5106..5423)
                     /locus_tag="P5549_02400"
     CDS             complement(5106..5423)
                     /locus_tag="P5549_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW11956.1"
                     /translation="MRQVLLKYCGGCNPTINRSKIIAEVKRGLPPDVELVSKTADQVA
                     EVGIMMSGCSTTCLDREDVRKQAQQWIVVGGNNVNYFPVENDQLADQIIKKIIDCLPP
                     ASK"
     gene            complement(5430..6902)
                     /locus_tag="P5549_02405"
     CDS             complement(5430..6902)
                     /locus_tag="P5549_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRW11957.1"
                     /translation="MTLKTPQQYLDDLRNMKIDLYMFGEKVENYVDNPIIRPSINAFA
                     MTYKLAQEPEYEDLMTATSSLTGHRINRFTHLHQSPEELMKKVKMLRLLGQKTGCCFQ
                     RCVGMDAFNAVFNTTFEIDAKHGTQYHERFKKWLEYVQDNDLAVDGCMTDARGDRSKT
                     PGQQPDPDAYLHVVERREDGIVIRGCKLHQTGMINSHEILVMPNNTLKDDEKDWAVCC
                     AVPLDAPGMIYVYGRQASDTRKLEGGNIDIGNIYFGGQEVMTIFENVFVPNDRIFMNG
                     ETDFSTMLVERFAGYHRQSYGGCKVGVGDALIGATKAMADYHGVSKASAVKDKIVEMC
                     HLNETLYACGIACSAEGRKTAAGNYLIDLLLANICKHNVTRFPYEIARLAQDIAGGYF
                     VTMPSEKDFAHPEIGPLVKKYSTTSTTVPAEHRQRMGRLIENMTQGAAAVGYLTESMH
                     GAGSPQAQRIMITRLVDLEHRKELAEKLCGVKENNDVDWK"
     gene            7535..8833
                     /locus_tag="P5549_02410"
     CDS             7535..8833
                     /locus_tag="P5549_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW11958.1"
                     /translation="MDWKSYYSDHIRTPAEAVKAIKSGDRVVLGHACGEPQALTRALV
                     ARAQELENVEIIQRVAMGESSYVNPEMQGHFKLNSLFGSAPTRKAIADGRADFTPSFI
                     SEIPGLFLTGRIPVDVALIAITPPDNHGYCSLGISIDYTIQAARAAKIVIAQVNPNMP
                     RTYGDTLLHVSEIDYFAPAEEPLIVLDRPVIGPAEKQIGMNVASLIEDGATLQLGIGS
                     IPDAILSFLYDKNDLGVHTELFSDGVRDLVEAGIINGKKKTLHPNKVVANFIMGTPKL
                     YDWIHDNHAINMYPEDYVNDPWVVAQNYHMTAINSALEVDVLGQVAADTLGPMQFSGV
                     GGQVDFVRGAARSVGGKSIIAFPSTARKGKTSRIVAVLARGSAVTTSRHDTDYIVTEY
                     GIASMKGKTVKERMEALINIAHPDFRSEIRKDAYNTYFSR"
     gene            9015..10280
                     /locus_tag="P5549_02415"
     CDS             9015..10280
                     /locus_tag="P5549_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW11959.1"
                     /translation="MRFRNKRYIGLLVALVFVLSLVAGCGGGGGTDKEAAKEIKMGTI
                     LPLSGSAAPLGKIGQEARDMAIEEINAAGGIKSMDGAKLKMVYSDSKGDPATGVTEAE
                     KLITQEEVVLLTGCYQSGVAMPSTEVAERYQIPYFVDVPSEDKITERGFKYIFRLAEK
                     TSWRNKAQTEFITEMSKKYNTPIKTVGLIYENTAWGQGAVTAWEKYLPAAGLQIVMKE
                     PYPANSSDLTPVVLKAKQLNPDIIMMVSYVSDAALLTKTFAEQKVTPKAFIGTSGGFA
                     DPTYYNLAGDACENFFDISTWEPDVNRPTSKETNEKFKQKYGYAMNAEAVKAYVGMYV
                     IADTLERAASTEPEKLREAFAATDLKSGPAQMYAAETKFDETGQMVNAPIVICQYQKV
                     NGKMERVTVYPQAEARPGSQIVWPYPGLK"
     gene            10359..11240
                     /locus_tag="P5549_02420"
     CDS             10359..11240
                     /locus_tag="P5549_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW11960.1"
                     /translation="MGFLSVVANGLSMGAIYGLIALGLTLIFGIMKIINFAQGALLMV
                     SMMISYWLWVATGLSPYILVLIVAPLMFLMGYFSERFLIKPVIDAQKDVREPVSVLLL
                     TAAFAIVLENLCLMLFGADYVMAQTSFSSQTLALGSVTVSAARFYGLIIALVVAFLFY
                     MFLQYSQTGRNLRATGQDRNAAALMGIDVTKTYAIAFGIGTALLAVAAVALIPFYYVH
                     PSVGAVFMTKAFIVVVLGGLGSVPGAILGGIIIGLIESVAAQYMTATLTAMLVYLVFL
                     VVLFIKPTGIFGSPYEW"
     gene            11250..12281
                     /locus_tag="P5549_02425"
     CDS             11250..12281
                     /locus_tag="P5549_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW11961.1"
                     /translation="MKGINKNYIILAGLGILLCLLPMFVTKPYYLQVIIMCILWAYLA
                     SCWNIIGGFAGQLSLGHGIYTAVGAYVAVILFNTFGLSPWLGMFVGGGIAVLLSLVIG
                     FPTFGLRGAYYALATVAIGEAVVVLLNNTGHLGQFLIGGAEGLSVKMVGVSAAAIQFN
                     SKVPYYYIILAMLFLIIFIAQWIKNSKLGYYLVALREDEDAAAAMGIDVRRNKLIAGA
                     ISAFFTALGGVFYAVLIRYLEPTAIAGAIMSNQMVFMTIVGGAGTVLGPVIGGITLSF
                     ISELIRFYLGSQLMGLHLFIFGLIVMLMIIFQPKGLIELKERFKRKRGSGADQNEGGE
                     SGARSASGA"
     gene            12256..12987
                     /locus_tag="P5549_02430"
     CDS             12256..12987
                     /locus_tag="P5549_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW11962.1"
                     /translation="MQEALLELKDLTISFGGLTAVNKLNTKLYSGEAIGLIGPNGAGK
                     TTAFNLIVGVYQPTAGQVFFNGEEITGLNPHEVCRKGIGRTFQVVKPFGKMTVLQNIM
                     VGAFVNTSREKEAKRVAIEIMEKVGLQRKKDELAQNLTIPERKRLEVAKALATKPKLL
                     LLDEVLAGLNPTEIEDAIVLIKSINQEGITVLMIEHVLQATMAICERIIVLDYGKKIA
                     EGTPEEVTSNSEVIKAYLGDEYENA"
     gene            12974..13684
                     /locus_tag="P5549_02435"
     CDS             12974..13684
                     /locus_tag="P5549_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW11963.1"
                     /translation="MKMLEIKSLDSGYGDVTVLKNVSLHVDEGEVVSIVGANGSGKTT
                     LLNTISGILPTKRGEIWFGDQRVDQLPPHKITELGIVQVPEGRRIFPLMTVYENLMIG
                     AALPRAKSKRNENLEYVFELLPRLKERKDQVGRTMSGGEQQMLAIGRALMAMPRILML
                     DEPSMGLSPLLVSTVFEVISKIGDQGITVLLVEQNVKRALGLSQRGYVLENGFISMEG
                     PSSELLVNPHIKKAYLGL"
     gene            13945..16026
                     /locus_tag="P5549_02440"
     CDS             13945..16026
                     /locus_tag="P5549_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW11964.1"
                     /translation="MLAKDIMIPTPLQCHQKMTLTEVSGMLLENHLDGLPVVDDGGDF
                     VGYFGRRQLYKALHQHKDPQMPVRELMITNVMTVSPDTALTDLYPLHNTIMPVLEKRK
                     ILGVITRELVAMYSNLEAHGYSPQTMATFDAAYEGIIIINNDLQVLAFNRAAEEILGI
                     KRENILNRRYPEVFPRGNLHRVIESRQAIANEKFVHHQRTYLSINSPIELAGNLIGAI
                     AVINDISTLESVTHELEYTKKLKSELDAIIEASFDSIFVTDAKANVISINEAYTRITG
                     ISAEEIIGKNMYDLVEQGLYDRSATIHVIENRTPITFAQNIKSGKTLLVTGNPVFNEQ
                     GELAWVITNGRDITELMRLKQEVELAQNLSRHYEEELKKVVQSGEMVVNSPKTKELLE
                     LAMRIGKVDSTVLISGESGVGKELIARELHKNSNRKDKPLININCAAIPESLLESELF
                     GYESGAFSGARKGGKMGIFELANAGTLFLDEIGEMPLTLQSKLLRAIQEKEILRIGAT
                     TPIKIDVRLITATNRDLWEMTRRGQFRQDLYYRLNVVPVYVPPLRERKEEIPALIYHF
                     VNLFNEQYNLNKKIDERMITSLLEYEWPGNVRELKNAIERAIVTSPEEVIRTIQLGGN
                     VNGGEMEFNTEKTENSGIDLKEKVNAYEKDLLQRYIQKYKSSRKVAKALNVSQTTVVR
                     KAAQYGIPLEG"
     gene            16301..17443
                     /locus_tag="P5549_02445"
     CDS             16301..17443
                     /locus_tag="P5549_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW11965.1"
                     /translation="MDFKMTEEYEMIRQSVRDFAEGVLAPGVVDRDEKEYFDRTLFDK
                     MGELGLTGIPFEEKYGGAGMDYVAYAIAVEELGRVDASAADTLSSHLSLGTWPIYAFG
                     TEEQKMKYLVPLAEGTKLGAFGLTEAGAGSDAVAMTCKADLDGYSYVLNGRKIFITNA
                     EAADTYIVFARTGPKELRSKAFSAFIVEKGTPGFSFGKFEKKMGLRGTQNCDLVFEDC
                     RIPKENLLGQEGEGFKIAMATLDGGRIGIAAQSVGVAQGAFEIARDYSKQRIQFGKPI
                     STLQAIQFKLADMMVDIKASRLLTYDAAWRKSQHMEYGVDAAIAKLKASETANQVANQ
                     AVQIHGGYGYTREYPVERIMRDAKITELYEGTSEIQRVVISNNVLR"
     gene            17460..17882
                     /locus_tag="P5549_02450"
     CDS             17460..17882
                     /locus_tag="P5549_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRW11966.1"
                     /translation="MDYSDKIKHWDDLHIGDAATFTKTITETDVILWVGLTGDLNPIH
                     IDKEYSKTTRFGDVIVPGVMVLGLISNTITMATFGNVYANQSIKFLKPVYIGDTITAT
                     ATIVEKLEAKHMVKLETKCENQKGELIMVGEGMEYILS"
     gene            18364..19548
                     /locus_tag="P5549_02455"
     CDS             18364..19548
                     /locus_tag="P5549_02455"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRW11967.1"
                     /translation="MTREVVLVGACRTPVGSFGGALKDVSVVELGSLVMNEALQRAGI
                     EAACLDEVIFGCVLQAAQGQNVARQSAVNAGIPIDITAMTINKVCGSGLRAVSLAAQI
                     IKAGDADVILAGGMENMSAAPFALEKARYGYRMNNGTLVDVMIKDGLWEAFNNYHMGI
                     TAENINEQYGISREEQDAFGLRSQERALQAIETGRFNDEIVPVTIKGKKGDQVFAIDE
                     FPRATTAQKLAGLKPAFKKDGSVTAGNASGINDSAAAVIVMSREKADEMNIKPMARVL
                     SYASGGVDPKVMGLGPIPATRKALAKAGLTIDDIDLIEANEAFAAQSIAVARDLGFEN
                     HMDKVNVNGGAIAIGHPIGASGARILVTLLYEMQKREARKGLATLCIGGGMGTAMVVE
                     KC"
     gene            19609..21444
                     /locus_tag="P5549_02460"
     CDS             19609..21444
                     /locus_tag="P5549_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW11968.1"
                     /translation="MPHYNYLYQTRDIKFQMKEWLDMDKLLSCDGYKDYYGADDIDAF
                     LEVNAKISRDVICPANKDSDEIGMKFVGGEKQAVISPDSFKTVYNTVVEAGLGPQFGD
                     RQSEGRIPLSWYAPILEQQSGASPAMVMLWCLTQGATTVLQFEGTEKQQQLFLPNMFA
                     GKWGGTMCLTEPGAGSEVGNCSTKAFATDTRGLYKIKGLKCFITSGDHDCVENFVHLV
                     LAKTEGAKAGTAGISLFIVPKFWVNDDGTMGDWNDVTTVGIEHKMGIHGSSTCTLAFG
                     ENNHCLGWLVGDELPVDGRGKGMAQMFRYMNEERLNTGLFSLGAFSSAYYAALEYAKV
                     RVQGRKSTDSKGSQVRIIEHEDVRRMLLYQKACMEAIRALIYTTYYYVDLSHEANDPA
                     DREYADDMFMINNPVCKAYASDMAWSLTQEAIQVHGGYGFMEEYAPASLARDCKIYSL
                     WEGTNFIQAQDFTGRKFTMKNGEPFKKWVGEIEEFVARQKMPEFAAEFALLEEALQAF
                     KAIVAMNDDWQNTNKQLKQLFATRTLHAAARVYCGKLMLDQGLLAASQLAQLGDEHYD
                     ANFYKGKIASVKFYVLNVVPEVFGYERAMKESDTSAIDIPEEAFM"
     gene            21647..22132
                     /gene="coaD"
                     /locus_tag="P5549_02465"
     CDS             21647..22132
                     /gene="coaD"
                     /locus_tag="P5549_02465"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRW11969.1"
                     /translation="MAIAVYPGSFDPVTMGHIDILERTSRIFDKIVVAVVHNVTKQAL
                     FTLDERVELIKNSVQHLPNVEVECFNGLLADFLKSKDATVIIRGLRSITDFEYESHMS
                     MMNKNLLPDIDTVFIMSNPCYIFVSSSGIKEAALLGGSVCGLVPKAVEIGLKEKLHRF
                     K"
     gene            complement(22184..22822)
                     /locus_tag="P5549_02470"
     CDS             complement(22184..22822)
                     /locus_tag="P5549_02470"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRW11970.1"
                     /translation="MKRIKLIIPVIMLSLLFTGLLFTQSEKAMASANLSWGSHGTEVV
                     TLQKSLNNQGYWCGEADGVFGPKTYAAVIKLQKAQGLDTDGIVGPKTKTALGLSDNSN
                     VTSTPSRSGSSVNRAKTLTMTATAYCSCYKCNYPYYGAPSYIGLPLGRGIIAVDPRVI
                     PMGTRLYVEGYGEGIAADQGNAIKGNRIDLCFATHQQALSYGMKTVKVTILD"
     gene            complement(23109..23639)
                     /locus_tag="P5549_02475"
     CDS             complement(23109..23639)
                     /locus_tag="P5549_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRW11971.1"
                     /translation="MPFYEFEEKRPTIGENSFVHPQATVIGDVKIGNQCYVGAGAILR
                     GDYGTIEIGNGSNIQENAVLHAEPGTCAKIGEDVLVGHMAIVHGPCTILNRVIIGMGS
                     LISTGCEMGEGSFLAAGSVLPPGRIIPAHQLAIGNPAVAVKEVSGTLAQYNGIAVKLY
                     QDLAIRCKTGLKQIDL"
     gene            23816..24703
                     /gene="hslO"
                     /locus_tag="P5549_02480"
     CDS             23816..24703
                     /gene="hslO"
                     /locus_tag="P5549_02480"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRW11972.1"
                     /translation="MQNQDYLIKLIDAGQNVRVWLARTNRLVEEAHIRHQTSATATAA
                     LGRVLTAAVMMGSDLKSDQDLISLRFNGNGPAGTIIATADGNGHVKGLIGNPQADLPS
                     RSPGKLAVGDLVGAEGYLEVRKDLGLKQPFEGKVPLVSGEIAEDLAQYFMQSEQIPAL
                     VSLGVLVGADLRVLAAGGLIVQALPNAEDAVLEILENNILSLGQISQLVLENETLEDI
                     AASIMQGIEYTIIARMNLAFACNCSRERLAIILAGLNEEEIREAAENNKDIEAVCNFC
                     RTTYTFSPDEIKALKSEKP"
     gene            complement(24738..24926)
                     /gene="rpmB"
                     /locus_tag="P5549_02485"
     CDS             complement(24738..24926)
                     /gene="rpmB"
                     /locus_tag="P5549_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRW11973.1"
                     /translation="MAKCEICGKGVTFGKKYSHSHIRTNRQWKPNVQRVKVLDDKTPR
                     RMYVCTRCLRSGKVQRAL"
     gene            25044..25406
                     /locus_tag="P5549_02490"
     CDS             25044..25406
                     /locus_tag="P5549_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRW11974.1"
                     /translation="MFTIKTNDLGNITVEKEVVETIAGLAALDSYGLVGMVPHNLQSG
                     ITHMLGLESIRKGVEVKNGTDGLIVDVHVIVGYGIKISEVALSVMQKVTYVLEKKAGI
                     PVEKVNVNVEGIRVIKDK"
     gene            25423..27045
                     /locus_tag="P5549_02495"
     CDS             25423..27045
                     /locus_tag="P5549_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRW11975.1"
                     /translation="MSLLSITGYDLVRCIKAGCIRLEHNRDEVDQLNVFPVPDGDTGT
                     NMYLTLLSAVREGEKNSTASLGKVAKAVSMGSLMGARGNSGVILSQIFRGFAKTLENK
                     ETANAVDLALALRSGADTAYKAVMKPVEGTILTVIREIARACEQEAQSGSDILAVLLI
                     GVEEGYRTLEKTPSLLPILKEAGVVDAGGRGLLFFLEGVIEGLAADKEIQLDSYKEKT
                     ITSRQEMIKQDIVLDFQYCTEMLIKGQDLNPEDIKDHLRPLGDSMLVVGEEEIVKVHI
                     HSNHPGKVLETALQFGQLSDIKINNMLEEVHENRHDFEPNNLSAFCKKIGLVAVAAGE
                     GIVSILQSLGVDQVVQGGQSMNPSTEDLLKACQDVPAQAVIIMPNNKNIIMAAEQVKE
                     LSDKQVTVLPTRSVMQAIAALIAYDSEGELDVIMAAMQAETEKVSYAEVTHAVRDSGV
                     SGLEIEAGNVIGLIDGKISQKADSEEEVVLAILRNMIKPDSELITFLYGNGLEEAEAS
                     ALQEKVMEICPQCEVEMHFGGQPHYSYFISVE"
     gene            27142..29187
                     /gene="recG"
                     /locus_tag="P5549_02500"
     CDS             27142..29187
                     /gene="recG"
                     /locus_tag="P5549_02500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRW11976.1"
                     /translation="MESLFMELQYIKGVGPKRKRDLQKLGVDNIFDLLWLVPRSYFNR
                     SHLTPICDLPLDTTVNLQARVLSTTSTRTGRGLQIFKALLEDDSAAIHAIWFNQPFLT
                     NVIKTGQQILVTGKVDGRYKELQLQVSAYEILEDDEVMAFSVVPIYPLSGSLNQKSLR
                     KIMNYVLQNFLTDYPEILDADLREKYELLDIHSALHHIHFPENAEKYLQARRRLALEE
                     LLLFQISLDKHRTDRQPAAPCVIHLEKTNLVQHLRQSLPFRLTDDQEKVVGEIYADMQ
                     APRTMNRLLQGDVGAGKTVVAALAMAKAAASGYQAALMAPTEILAQQHFAALQRLFAG
                     APVEMACLTGGTSQAQRRIMLEAVQRGEIDILLGTHALIQEQVEFRRLGLVIIDEQHR
                     FGVRQRARLGQKGINPDVLVMTATPIPRTLALTAYGDLDLSVIHQLPPGRKPVKTRYL
                     PRSSRSKAYDFARPMIQQGMQCYVVCPLVEESEKQDMQAAITLYEELRTHIFAEFSVG
                     LLHGRMKANEKEMIMQRFQAGQIQVLVSTTVIEVGVDVPNASVIIIEHAERFGLSQLH
                     QLRGRVGRGSHQSYCILLADARTEEAKRRLQAMEQSNDGFELANEDLAIRGPGDFWGV
                     RQHGLEELKVASLVKDLELIRLSMELAGQLEIAYLQTPFLQLYMQLKFKKNHEIARN"
     gene            29323..29460
                     /locus_tag="P5549_02505"
     CDS             29323..29460
                     /locus_tag="P5549_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HRW11977.1"
                     /translation="MKKTSGQQQPKKPSTNDAGNQAMENLKYEVAQEMGIKPKTKNKK
                     S"
     gene            29532..29738
                     /locus_tag="P5549_02510"
     CDS             29532..29738
                     /locus_tag="P5549_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HRW11978.1"
                     /translation="MARNSNRLLVPQAKAAMDSFKYEAAQQVGVNLKQGYNGDLTSAQ
                     AGSIGGQMVKKMIESYETGKSTMK"
     gene            complement(29824..30783)
                     /gene="gpr"
                     /locus_tag="P5549_02515"
     CDS             complement(29824..30783)
                     /gene="gpr"
                     /locus_tag="P5549_02515"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HRW11979.1"
                     /translation="MNDFLNHFNLNVDLAVEARDLVRGTTGREIQGVEEQVEKMEQIK
                     ITTITIKDPQAVAVMGRPLGTYVTIESPPLKINDPYVKDEIITAMNKSMEVLLNNRLQ
                     PKDTVLLVGLGNWRATADALGPKFIEYSPITRHYFQHAPEALVEGMRPVSGIAPGVLG
                     ITGLETYEVIKGVVDNVKPAVLVVVDALAAQNVDRIGNTIQMSNTGIQPGGGVGNARH
                     ALTEEELGIPVIAIGCPTIVNAAVIAQQAINNFCSVSNTVFHDMTVTQSVKDTLSYFG
                     GDLAVTPKEVDEIIENTARIISMGVAASLFPGISREQLELYAT"
     gene            31076..31666
                     /gene="rsmD"
                     /locus_tag="P5549_02520"
     CDS             31076..31666
                     /gene="rsmD"
                     /locus_tag="P5549_02520"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRW11980.1"
                     /translation="MISGYARGKKLKAPSGLHTRPITDMIKEALFNVWGPGIVNASIL
                     DLFAGSGSVGIEALSRGAAQAVFVDNDKNAVKVMAENLRICGFDNGYELYHNDVFKAV
                     NTFSRNRRCFDYIYVDPPFTEDKIFDQIMMVLGQADILARQGALVIRTRRQRELAPSF
                     GKLNQYRKKNYGESTLHYYRYMEEDQNDDGNISDLG"
     gene            31638..32105
                     /locus_tag="P5549_02525"
     CDS             31638..32105
                     /locus_tag="P5549_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRW11981.1"
                     /translation="MMEIFRILDELELLLKGSRKVPLTGGKSIIETSRFLDRVDRIRA
                     ILPEELETAKLIMAEKERIVSEACAEADKYLEHSKSHVAQMVDDNEITRSARGAAEEI
                     IAQSEQIAAQIRRDADEYADGVLSHMEIVLRKGLEAVLQGRDDIREGLRSNDY"
     gene            complement(32099..33280)
                     /locus_tag="P5549_02530"
     CDS             complement(32099..33280)
                     /locus_tag="P5549_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HRW11982.1"
                     /translation="MSNVRFPLSVIIILTGTFFMIIHPQATVQAASQGLLLWYSIVLP
                     ALLPFFIVAELLVKIRFVNFMGVLMEPVMHPLFRLPGCSALVVVLGFTSGFPVGAVLT
                     RKLYDQQQLSTEEAERLLVFTNNASPLFILGAVGIGLLSNPAAGYLLAFSQYLSNLLL
                     GILLRFKAPRSAPPIPPGGSGWKAACQASLFDNGERSGFAGMLGDAIRDSLGNVMAVG
                     GFIVVFSVLTHMLSQWGLLEVMAGLLFSLLNIFPLDYAQVYGLCMGIFEMTLGAKTAA
                     IIVPGNMLAVLLVLSAILAFSGISVIAQVMSIMAGTSIRLSFYLLSRLLQMAMACSIT
                     FLAYRLALLTGMAGIQVQAIPYHKVLYAFDAWTWSLYSLLAAFIIIAVLLCMATGRQH
                     Q"
     gene            33358..34146
                     /locus_tag="P5549_02535"
     CDS             33358..34146
                     /locus_tag="P5549_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRW11983.1"
                     /translation="MENSKPVVGLVLGAGSARGLAHIGVLQVFREKGIKVDFIVGSSM
                     GAMVGAIYASGADLYMLEKMLECMNPNLMIDVQVPRYGFVAGKRIDAFLTLLTKNKSF
                     DQLDPPVVAVATDLITGARVVLDQGPVREAVRASISIPGVFTPVRKEGMVLVDGAVID
                     RLPVEVAKDRGADIIIAVDVTFGPDKKVTIKNTLDVIMTSLDIMQKYHFSLIGSQANV
                     LIQPAVGNFASRDFECSKDIIALGRAAALAKVEEIKQLTDSFKS"
     gene            complement(34136..35377)
                     /locus_tag="P5549_02540"
     CDS             complement(34136..35377)
                     /locus_tag="P5549_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRW11984.1"
                     /translation="MTALGIIAEFNPFHNGHLHLLQAAKKAVPDAPIVCVMSGNFLQR
                     GEAAWCSKWSRAAMALHAGADLILELPVCFAVRSAYYFARGAIQLLAQTGIITHLVFG
                     SESGNLNELQKISAVISNESPEYQMILKQHLSRGYSFARARSLTLQKIIDNPQLNDLL
                     LQPNNILALEYLRVLNELHSSIIALTFPRQGTGYHSNKLSDWASASAIRAALDKEQNL
                     AAIASSVPPYTLQILAEEISHGRAPIEAEPLHQSILLKLRLSSAAKLADIYEVEEGLE
                     NRIVKAALQSVNLAELKQKIKSKRFSQSRINRILLYCLWELDKAQIIRFDECGPQYLH
                     VLGFSPSGQKILQEIKSQTDLPIFSRGSDFKKACRQTNQPMLTEMLALDRQASDVYSL
                     LYPDPAVRTGGLDFTTSPVRI"
     gene            35611..36603
                     /gene="pta"
                     /locus_tag="P5549_02545"
     CDS             35611..36603
                     /gene="pta"
                     /locus_tag="P5549_02545"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRW11985.1"
                     /translation="MSLLAEIKEKASKKGKTVVLPEGTEERTLLAVKGICDGKIANPI
                     LLGNESDVKAAASKAGADISGAVIIDPVKAPYFDDFVQAFYEMRKSKGVDMDKAKATM
                     QDPLFFASMMVQMGKADGEVAGAENTTGNVLRPALQIIKTAPGISAVSGSFIMIVPNS
                     EFGDKGIFVFADCAVTPMPTAAQLAEFAKTSAQTAVNLCGMKEPKIGMLSFSTKGSAD
                     HEVVDKVVEATRIAKERFPELAVDGELQADAAMVPKVGSSKAPGSLVAGQCNVLVFPD
                     LQAGNIGYKLVQRLAKAEAIGPILQGMAKPVNDLSRGCSVEDVINTVAMVVMQS"
     gene            36649..>37745
                     /locus_tag="P5549_02550"
     CDS             36649..>37745
                     /locus_tag="P5549_02550"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRW11986.1"
                     /translation="MKVLVVNAGSSSIKYQVFNMEDESVLAKGLVDRVGIPGTTLEHK
                     PTGKDEVIIKKDLPDHTAGMKLVLEVLANSEYGVISDMAEIGAVGHRVVHGGESFADS
                     VVIDDAVKKVIKDCFEIAPLHNPPNLMGIEACQELMPNVAHVAVFDTAFHQTMQAENY
                     LYALPYEVYEKFKVRRYGFHGTSHYYVAHRAADMLGKPYEDCKIVTLHLGNGGSMAAI
                     KDGKVVDTSMGFTPLEGLVMGTRSGDIDPAIVWFLMDKLGMDTAEANNYFNKKSGMLG
                     LSGVSNDLRDVLEAAGSGNERAKIALQVYYNQVKGYIGNYMAKLNGCDCLVFTAGVGE
                     NGYDVREAICADMDYLGVKMDNEKNKVRGKEV"
BASE COUNT        10371 a         7947 c         9699 g         9728 t
ORIGIN      
        1 attttagtga aacaggacac tgctgctgat gaatatgcca gagctgtagc cctggccgat
       61 gcaatcaaag cagaaagccc ggatttgatc ctggctggtc acgtagcttc cgatgatggt
      121 tcttcacagg ttcccacccg tattgctgaa attttaggcc tgccgcatgt taacgttata
      181 accagcattg aaatcgctga cggaaaagct acctgcacca gtgaagcaga tggcggtact
      241 gctgtaaccg aagttgccat tccggcagtt atttccagcc aggtaagctg gaatgaaccc
      301 cgttatcctt ccatgaaggg tatcatgcaa gccaagaaaa aaccggttgc tacggttgat
      361 gcagtagccg ttgacaacaa ggttaaaata gttgaagttg gtctgccggc tggtaaaact
      421 gccggtatca agatcgagga agatccggac gtagcagccg ccaagttggc tgactggatg
      481 aagaataccg taaaggtaga agtaaaataa tttatacgtt tgtataaata aaggagggta
      541 attgatggct ggaacatggg tttttgttga tcaaagagat ggaaatatac gcaaagtttc
      601 atatgaatta ctatcagaag ccaagaaatt tggcggagaa gtagcagccg ttatatttgg
      661 tcagggcgtt gaaggcttgg ccggagaatt tgcaaaatat ggtgctgaca aggtttatgt
      721 agcggatgac gctatatttg cagcatacaa tactggtgct tatgtggctc agatcgttgc
      781 catgatcaaa gaatacagcc ccaatgcggt attgttcgga cacaccataa acggccgcga
      841 catggcttcc agactggctc agaaattgga tgcaggtctg gcaactgatg ctattgctgc
      901 tgagatttca gatggcaaag gcatttttac cagagcgatc tatgccggta aagccatcgc
      961 taaagtcgaa gtaaccggca gtcccgtagt ggcaaccatt cgtgccggtg tttgggaagt
     1021 tgttgaaggt gctggtgccg gcgaagtagt taaagcagca atcgctgcta cagctgccga
     1081 tgtttaccag gcagccaaag agtttattcc tacggtttcc gccagaccgg aactgactga
     1141 agctgcggtt gtagtatccg gcggacgtgg atgtaaaggt ccggatggaa tcaaagttgt
     1201 tgaacagctg gctgatctgc tgggcgcagc cctgggcgga tcacgtgcat ccatcgattc
     1261 cggctggttg ggccatgaac tgcaggttgg acaaaccggc aaggtggttt gccctggtct
     1321 gtatgtagct tgcggcatct caggagccat tcagcatctg gctggtatgt cttcttccaa
     1381 gtttatcgct gccatcaaca ccgacactga agcacccatt tttggcgtag cagatttcgg
     1441 cgtagtaggc gatttgttca aggttgttcc gctcctttgc gatgaactga agaaataatg
     1501 gataatgaaa ggcttccgga tatgccgggg gtgtatccgg gagacttttc ttattcgaca
     1561 ggtgctcaga attaatggcg tgattagcag caagtattga ttctggtgtc gccaaactcg
     1621 gcacatccgg gttaaatttt gagggaggga agttttttca tgatttttgg ttttaaacct
     1681 ctggaaggcg gctacgatat tcccatgcgg gaagtggggg ccaacattcc cttcgactgg
     1741 ctgatttata ttatcatgtt tatccccatc ggaatatttc tttatggtgc ctacaaaaaa
     1801 tatctgatgt ggacgattgg aaaaggtgaa attcacagaa acgataaagt tggcgcccgc
     1861 acggtgtcat ttttagtgaa cagcttcctg caagccagag tgataaaaaa accgctggcc
     1921 ggctggatgc atttcttcct tttctgggga ttcctgttct tgtttatggc tgccggcata
     1981 gatgccgccc accattggct gggatggcca catttggaag gtttccttta tatgggtatg
     2041 tcctggattg ttgatgttac tggattcctg gccatgatcg gtattatggt tttggcttat
     2101 atccgctata ttcaaaaacc tgatcgttta aatgacacca aagcctctga tggctggatc
     2161 atcctcttga ttttcaccat tcttctgact ggatacttca ttgaagggct cagaatagca
     2221 gcccaaattc agctttctac aaccatggcg caaattgctt atgagagagt ctgttctcct
     2281 tttggctgga tgtttgccgc cctctttggc gggatgcatc ttgacagtat cctaatctgg
     2341 caccggttgc tgtggtggtt ccatatggcg atcgcgtttc tgtttattgc aattataccg
     2401 tttaccaagc tgtggcatat ctttgctgga atggtcaact atgtattccg cgatctggaa
     2461 ccctcggcct tacgttttgt tgaaaacatt gaagaagcag aaacatttgg cgtagagaga
     2521 attgaagaat tcggctggaa agatctgctt gacctggatg cgtgtattcg ctgcggacgt
     2581 tgtcaggaaa attgtccggc ctataacacc gggaaacatc tgaatcccaa aattgtaatc
     2641 atacaaaata tgagacaaca ccttgaggaa aaaatgcctt atctgcttga agccaaggat
     2701 aatcctgagg aagttgctga aattgctatg actgatgcag ctgctgaaaa ggtcagcccc
     2761 atggaaacga gtctaatcta tgatgtggtg actacggaag caatatggga ttgcaccaat
     2821 tgcagagcct gcatggaaca ctgcccgatg tatattgagc atattcccaa gatcgttgaa
     2881 atgcgcagaa acttggttat gtggcaggga gatatgcctg gtgaagccca aatggcattt
     2941 accaacatgg aaagaaacta caatccgtgg ggggttggct gggctggaag agctggatgg
     3001 ctggaagaaa gaggtgtccg ggatctggtt aatctgctgc cggaagacgg caaggaattt
     3061 gaatatctcc tttatgccgg ctgtgcggtt tcctttgatg atcgttacaa gaaggttgga
     3121 gaatcgctgg taagactgct tgacaaggcg ggagtaagct ttggttacct gggtacggaa
     3181 gaattctgct gcggcgattc tgccagaaga ctgggcaacg agtatttata ccaaaccttg
     3241 gtcagccaaa acctcgaatc ttttaacaat tacggcgtca agaaaattat cgttgtatgt
     3301 ccgcatggtt acaatgccct gaagaatgag tatccgcaaa tgggcggcaa ttttgaagtc
     3361 tatcattata ctgaaattct ggccaagctg gtcgctgaag gcaaactgaa acccgccaaa
     3421 tcagtcggtg ccaagatcag ttatcatgac tcctgtttcc tgggacgtca caacggcgta
     3481 tatgatcagc ccagaaatgt gctgaaagct gctggtgcat ccattatgga aattgatcaa
     3541 tccagaaact tcggattctg ctgcggcgcg ggaggtggtc gcatgtggat ggaagaagaa
     3601 gcagtggaag gattcaaacg tatcaatgat accagaaccg atcaactgct ggtaaccaat
     3661 ccgcaaatga tcgtaaccaa ctgccccttc tgtctgacca tgatctctga tggtgtcaaa
     3721 gctgctgaag cagaagaaaa caccaaggtg ttcgacgtcg ccgaagtatt gtggcagtca
     3781 atgcaataac tgccagatca acagggcatc tggttgatca aaatattgga atttaacaaa
     3841 acacaaggaa ttgcccctct gcctgtcaga cgaggggcaa ttcttttttg cttggaattt
     3901 ttcgtcgccg gccgtacaga cattactccg gacgtgtagc ttgaaaagcc ccgggcgcct
     3961 gattgaaaag cactaggcct tcttgcttta gcgcattgaa aatgccaagt ggaaaatctt
     4021 aatttatgta tgttcaacag aagaacgctt tccgattgac tgaaaaaaca gttaggacta
     4081 taatttatat atcaaaacga tatatgcgtg caaaaactat aaaatggagg tagaaggatg
     4141 gcaaattatc aacacgtatt ggttggcaaa gaaggaaaga ttgcatccct aaccgttaac
     4201 cgggcagaaa agttcaatac atttgggccg gacgtatggc gggaaatctt gctggctggt
     4261 caggaaatta tggaaatgga tgatcttgga gcgattgtga tcagcgccaa gggggatcat
     4321 ttctcagccg gaattgacct taaggatctg aataccttta attctgaata tgttttcaaa
     4381 tcgattccct gggcgcagtc ggtctattct ttttgggaag aaatacccgt tccggtggta
     4441 gttgccattc agggactttg ttatggttca gcggtggaac tcatactggc ttgcgatatt
     4501 cgcgtggctg cttcgaatgc ccgcttctca attcctgaag tacgtttcgg gcttgctcct
     4561 gacatgggcg gaacaactcg catgacaaaa ctggttggct caggacaggc taaacggatg
     4621 atcatgtgct gtgatgagat tgatgccgcc gaagcgaaat caatcgggtt ggtagagatt
     4681 gttgctgaac cagaaaatct aatgcaaacg gcaatgaagt acgccaataa actgacttct
     4741 cttccaccgg ttgcagttaa gatggccaag aaaggaatta atctggccgt tgacagcagc
     4801 gttagagggg gactcctttt tgagcaggct caaacgacct actgctgcgg taccgaagat
     4861 caaaaagaag cgattgcagc tttctatgag aagcgacagc cggttttcaa gggacgctga
     4921 ttggtttatg aatgccaggt ttgattgcaa aaacagtcaa taataggtgt tcaaaatgga
     4981 ttcttcaaag gaagaattaa aaagcagaga ttaaagaaaa caaccccctg aagcagtgtt
     5041 taaattctgc ctcagggggt catttttaaa gggatttcag tcagtcgatc agatctgtaa
     5101 aattttcatt tgcttgcggg gggaagacaa tcgattatct ttttgataat ctgatcggca
     5161 agctggtcat tttcaaccgg gaaataattt acattgtttc ctccgacgac gatccattgt
     5221 tgggcttgtt tacgaacatc ttcccgatcc agacaagttg ttgagcaacc cgacatcata
     5281 atacccacct ccgctacttg atctgctgtt ttgctgacca attcaacgtc aggaggaagg
     5341 ccgcgtttga cttcggcaat gattttggaa cgattaatag taggattgca gcctccgcaa
     5401 tactttagca gtacctgcct catacaaaat tatttccaat ccacatcatt attttctttc
     5461 acgccgcaaa gtttttctgc cagttctttg cgatgttcca gatcaaccaa acgggtgatc
     5521 attattctct gcgcctgggg agaacctgcc ccgtgcatgg attcggtcag gtatcccacg
     5581 gcagctgcac cctgcgtcat gttttcaatc agtcggccca ttctctgacg atgttccgcc
     5641 gggacggttg tgctggtggt ggaatatttt ttgaccagag gcccgatttc tggatgagca
     5701 aagtcttttt ccgaaggcat ggttacaaag taaccaccgg caatatcctg tgccaaacgg
     5761 gcaatctcat atgggaaacg agtaacatta tgcttgcaga tgtttgccag cagcaaatca
     5821 atcagataat tgccggcggc agtttttctg ccttctgcag aacaagcaat accacatgca
     5881 tacaatgttt cgttcagatg gcacatttcc acgattttat ctttcactgc tgatgctttg
     5941 gatacaccat gataatcagc catggctttg gtagccccaa tcaaagcatc gcctacgcct
     6001 actttacagc cgccgtaact ctgacgatga tacccggcaa accgttcaac aagcatcgtg
     6061 gaaaaatcag tttcgccatt cataaagatc ctgtcatttg gtacaaatac attttcaaag
     6121 atggtcatga cttcctgacc gccgaaatag atgttgccga tatcaatatt gccgccttcc
     6181 aatttgcgag tatcacttgc ctgtcgccca taaacataga tcatgccagg tgcatccaat
     6241 ggaaccgcac aacaaacggc ccagtctttt tcatcgtctt taagtgtatt gttcggcata
     6301 accaaaattt catgagaatt aatcatgccg gtctgatgca gtttacagcc acgaataacg
     6361 ataccgtctt cacgtctttc cacaacatgc agataagcat caggatcagg ctgctgtccc
     6421 ggcgttttag aacggtcacc acgggcatcc gtcatacagc catcaaccgc aaggtcatta
     6481 tcctgcacat attccagcca ttttttaaaa cgttcatgat actgggtgcc atgtttggca
     6541 tcgatttcga aagtggtatt aaagaccgca ttaaaagcat ccataccaac acatctctga
     6601 aaacaacagc cggttttttg gccgagcagt cttaacatct ttactttttt cattagttct
     6661 tcaggactct gatgcagatg cgtaaaacga ttaatcctgt gaccggtcaa actggatgta
     6721 gccgtcatga gatcttcata ttcaggctcc tgtgccagtt tataagtcat tgcgaacgca
     6781 ttgatggacg gtctgataat tggattgtct acatagtttt ctaccttttc accaaacatg
     6841 tagaggtcaa tcttcatgtt tcttaaatcg tccaaatatt gttggggtgt ttttagtgtc
     6901 atttctattc ccgcctttct aaataagcat tgtttcgaaa acctgagctg caaagaattg
     6961 tatgggatca ttttaataat cccagttaag tgtttcacta tttataacat gcaaaatcaa
     7021 tgccatacag attcaaacaa tcacaacctt tcgattgaaa tgctacaatc ttcgacgcag
     7081 cggttcttaa gtcatgcttg gatgtattta tataatgaag caacctattg taaaacaatt
     7141 cctgagtcag caacaggtca attcgcgata ccgttttgaa tgcgggaaat accgggtgcc
     7201 tgcataatgc ctaaatttcg ggaatacaat aagtgaacct aaaatgaatc aagaagacaa
     7261 gcagaaccga atatgaatca gcacgaagaa cgtcgaattg aagagcaatt tttgagaaaa
     7321 tgtaataaag acgaatcgaa aacggttcaa ttattgtgca taaacgcaca aaattgattt
     7381 cgtgcaggtg aatcaaatta ttttcatagt gaaagaataa caatattacc ggctttcgaa
     7441 gaaatattgt ccttgaaccg atctggtttc agctggttgg catgttgttt gctttaatat
     7501 aagtcaggag ccttaggaaa ggagcaggag agtgttggat tggaaatctt attatagtga
     7561 tcatatcaga actcccgcag aagcagtaaa ggctatcaag tctggagatc gggtcgttct
     7621 gggacatgcc tgtggggaac cccaggccct gacacgcgcc ttggttgcca gagcgcagga
     7681 actagagaat gtcgaaatca ttcagcgggt ggcgatgggt gaatcatcct atgtaaatcc
     7741 tgaaatgcaa ggccatttca aattgaattc cctgtttggc agtgcgccga ccagaaaagc
     7801 gattgcggac ggtcgggcag attttacccc tagctttatc agtgaaatac cgggtctgtt
     7861 tttgaccggt aggattccgg tcgatgttgc cctaatcgca attaccccac cagataatca
     7921 cggctactgt tctttgggga tctcgattga ttacaccatc caggccgccc gtgctgccaa
     7981 aattgttatt gcccaggtca atcccaatat gccccgtact tacggggaca ccttgctgca
     8041 tgtaagtgaa atcgattatt ttgcgccggc tgaggaacca ctgatcgttt tggaccgtcc
     8101 cgtgattgga ccagccgaaa agcaaattgg catgaatgtg gcttccttga tcgaggatgg
     8161 agctacgttg cagctgggga tcggttcaat cccggatgca atcctgagtt ttctttatga
     8221 caagaatgat ttgggcgtgc ataccgaact gttttcagat ggtgtcagag atctggtgga
     8281 agcgggaatc ataaacggca agaaaaaaac attgcatccc aacaaagtag ttgccaattt
     8341 tatcatggga acacccaagc tatacgactg gatacatgac aatcatgcga taaacatgta
     8401 tcccgaagac tatgtcaatg atccctgggt ggttgcccaa aactatcata tgaccgccat
     8461 caactcggcc ctggaggttg atgttctggg acaggtggcg gcagataccc tgggacccat
     8521 gcaattcagc ggagtgggtg gacaagttga tttcgtcagg ggggcagccc gctcggtggg
     8581 cggaaaatca atcattgcct tcccctctac cgcccggaag ggcaagacca gccgtattgt
     8641 tgcggttctg gcccggggtt ccgcggtcac tacctcacgg catgatactg attatattgt
     8701 aaccgaatac gggatcgcct ccatgaaagg caaaaccgtc aaggaaagaa tggaagccct
     8761 gattaatatc gcgcatcctg actttagaag tgaaatcaga aaagatgcgt acaacacata
     8821 tttttcccgt tgatatagtg ttccttgcaa tcctgttttg cattgcatag gtgaaaaaat
     8881 agtttgaagg aggaggggta tacaacaaat aaaatgatta tcgttgccag ccagggttgg
     8941 cagggaacgg ggcagatagc ctcgcggaga gttctcgttg tatgtgacaa aaaatatttt
     9001 aggaggtcag agggatgaga tttagaaaca agcgttatat cggtttactg gttgcattgg
     9061 tttttgtgtt gagtctggtc gcaggttgtg gcggcggtgg tggaactgac aaagaagccg
     9121 ccaaggaaat caaaatgggt actatccttc ccttgtctgg ttccgctgct cccctaggca
     9181 aaattggcca ggaagctcgt gacatggcca ttgaagaaat caatgctgcc ggtgggatca
     9241 aatccatgga tggcgccaaa ttaaaaatgg tttattcaga cagtaagggt gaccccgcaa
     9301 ccggcgtaac ggaagcagaa aaattgatca cccaggaaga ggttgttttg ctgactggtt
     9361 gctatcagag tggtgtggcc atgccttcca ctgaagttgc cgaacgctat cagattcctt
     9421 attttgtaga tgtaccttca gaagataaaa ttactgagcg cggattcaaa tatatcttcc
     9481 gcctggcgga aaaaaccagc tggagaaata aagcgcagac tgagttcatc accgaaatgt
     9541 ccaagaaata taataccccc atcaaaacgg ttggtctgat ttatgaaaac actgcctggg
     9601 ggcaaggtgc ggttacagct tgggaaaaat atctgcctgc agccggtctg caaatcgtga
     9661 tgaaagaacc ctatccggcc aattcttccg acctgacacc ggtcgtgtta aaagccaaac
     9721 agttgaatcc tgatatcatc atgatggttt cctatgtatc agacgctgct ctcctgacca
     9781 aaacgtttgc cgagcaaaaa gtaacgccga aagcatttat cggcaccagc ggcggatttg
     9841 ctgatccgac ttattataat ctagccggcg atgcttgtga aaacttcttt gatatctcta
     9901 cctgggaacc cgacgtaaac cgtccgactt ccaaggaaac caacgaaaag tttaagcaaa
     9961 aatatggtta tgccatgaac gccgaagcgg taaaagccta tgtaggtatg tatgttattg
    10021 ctgatacgct ggaaagagct gcttccactg agcctgaaaa attgcgggaa gcttttgcag
    10081 caactgattt gaaatccggt cctgcccaga tgtatgcagc tgaaaccaag tttgatgaaa
    10141 ccggacaaat ggtcaatgct ccgatcgtta tctgccagta tcagaaagtc aatggtaaga
    10201 tggagcgtgt aaccgtctat ccgcaggcag aagcaagacc tggttcgcaa atcgtttggc
    10261 cgtatcctgg tctgaaataa caattctggc aatgaggtgt gggactggcc ggtggcccgg
    10321 ccactcccca ttctcccaga gaaagggggt aataatcagt gggcttttta tcagtagttg
    10381 cgaacggtct tagtatgggc gccatatatg gactgattgc attgggactg acactaatct
    10441 tcggtattat gaagattatc aacttcgcgc aaggcgccct gctcatggtg tcaatgatga
    10501 tttcctactg gctatgggtg gccaccgggc ttagtccata tattttggtt cttattgtgg
    10561 ctccgcttat gtttttgatg ggctatttca gtgaacgatt cctgattaaa cccgtaattg
    10621 atgctcagaa ggacgttcgt gagccggtca gtgtgctttt gcttacggcc gcgtttgcca
    10681 tcgtgctcga gaatttatgc ttaatgttat tcggtgctga ttatgtaatg gcgcaaacca
    10741 gtttctcgag tcaaactttg gcgttgggct cagtgactgt tagtgcggcg cgtttttatg
    10801 gtttaattat tgctttggta gttgcatttc ttttctatat gtttttgcag tattcacaga
    10861 ccggacgcaa tttaagagct accggtcagg atcgcaatgc ggccgctttg atggggattg
    10921 acgtcaccaa aacctatgcc attgcctttg ggattggcac agccttgctg gcagtagcgg
    10981 cagtagcgct gataccgttt tattatgttc atccctcagt gggagcggtc tttatgacca
    11041 aagcgtttat tgttgtagtt ttgggtggat tgggtagtgt gcccggcgcc attttaggcg
    11101 gcattattat cggtttgatt gaatcggtag cggcccagta tatgacagca accttaacgg
    11161 cgatgctggt atacctggtg ttcctggtgg ttctgtttat taaaccgacg ggtatatttg
    11221 gctcacccta tgaatggtag gtgagaagaa tgaagggaat taacaagaat tatatcatcc
    11281 tggctggcct ggggattctc ctgtgtcttc tgcctatgtt cgtgaccaaa ccctattatt
    11341 tacaggtcat tatcatgtgt attctatggg cttatctggc atcctgctgg aatattattg
    11401 gaggatttgc cggtcagctt tccttgggtc atggtattta tactgcggtg ggtgcttatg
    11461 ttgccgtaat cttgtttaat acgtttggtt tgtcaccctg gctggggatg tttgttggcg
    11521 gaggaattgc ggttttatta agtttggtga ttggttttcc cacttttggc ttacggggag
    11581 cctattatgc cttggctacc gtagcgattg gcgaagcagt agttgttttg ctaaacaata
    11641 caggacatct gggccagttt ctaattggcg gagcggaagg cttaagtgtc aaaatggttg
    11701 gcgtatccgc agccgcgatt cagtttaact caaaagtacc atattattac attatcttgg
    11761 caatgttgtt tttaattatt ttcattgccc agtggatcaa aaattccaag cttggttact
    11821 atttggttgc gttgcgcgaa gacgaagatg ccgctgcagc gatgggcatt gacgtgcgtc
    11881 gcaataaatt gatcgcaggt gcaatcagtg ctttctttac cgccttgggt ggcgtgttct
    11941 atgcagtctt gattcgttat ttggaaccca ccgcaattgc aggagcaatt atgtccaacc
    12001 aaatggtgtt catgacgatc gttggagggg cgggtactgt tctcggcccg gtaatcggag
    12061 gaataaccct ctcctttatc agtgaattga ttcgcttcta tttagggagt cagttgatgg
    12121 gcttgcatct gtttattttc ggattgattg ttatgttgat gattatcttc cagcccaaag
    12181 ggctcattga gttaaaagaa aggttcaagc gaaaacgagg gtcgggggct gaccaaaatg
    12241 aaggaggtga gagcggtgca agaagcgctt ctggagctta aagacttaac catcagtttt
    12301 ggtggactga cagcggtaaa taaactcaat accaaactgt attcaggtga agcgattggt
    12361 ctgattgggc cgaatggtgc gggaaaaacc accgctttta accttatcgt tggagtatat
    12421 cagcccactg ctggacaggt attctttaac ggagaagaaa taaccggtct taacccacat
    12481 gaagtatgtc gcaaagggat cggtcgtacc ttccaggtgg tgaaaccgtt cggcaaaatg
    12541 accgtactgc aaaatattat ggtgggtgcg tttgttaata catcccggga aaaggaagcc
    12601 aaacgggtcg ccattgaaat catggaaaaa gtcggattac aaagaaaaaa agatgagctg
    12661 gcacaaaacc ttaccattcc cgaaagaaaa agattggaag tggccaaggc gttagccacc
    12721 aaacccaaat tactgctttt ggacgaggta ctggcagggt taaatcctac cgagatagag
    12781 gatgctatcg tattgataaa atccattaat caggaaggaa ttacggtttt aatgattgaa
    12841 cacgttttac aggccaccat ggcgatttgc gaacggatta ttgttctgga ttacggcaaa
    12901 aaaatcgctg aaggaactcc cgaagaggtg actagtaaca gcgaagtcat taaagcgtat
    12961 ttgggtgatg aatatgaaaa tgcttgagat taaatcactg gattcaggtt atggcgatgt
    13021 gaccgtattg aaaaatgttt ctttacacgt agatgaaggg gaagtcgtta gtattgttgg
    13081 ggccaacggc tcgggaaaaa caaccctgct caacacgatt tcagggattc tgccgaccaa
    13141 acgcggagaa atctggtttg gcgatcaacg ggttgaccaa ttgccgcccc acaagataac
    13201 ggaactggga attgtgcagg tgcctgaagg caggagaatt ttcccgctga tgacggttta
    13261 tgagaatctc atgatcggcg ccgccctgcc acgggctaaa agtaaaagga atgagaacct
    13321 ggaatacgta ttcgagttgt tgcccaggtt aaaagaacgc aaggatcagg tgggacgcac
    13381 catgagcggt ggcgagcaac aaatgttggc catcggcaga gcgttgatgg caatgccgcg
    13441 tatccttatg ctggatgaac cgtccatggg tttgtctccg ctgctcgttt ctactgtatt
    13501 cgaagtgatc agcaaaatcg gcgaccaagg catcaccgtt ttgctggtcg aacaaaatgt
    13561 gaaaagagcc ctgggattga gtcagcgagg ctatgtcctg gaaaacggat ttatttccat
    13621 ggaagggccg tccagtgaac ttttggttaa tccccatatt aaaaaggcct atctggggct
    13681 gtaaggcgca ggatagtgtt tggataaatt cccccctcct cagtagcgct atccctttgg
    13741 gccggaagcc tgaagggata gccttgttat gaatcgttga ttgcgatgga caaaaacatc
    13801 gtaaaaagaa tacatttcat aattatggtg ttaatagggt gtcaagattg gtagaattac
    13861 aaacaaatcc tgatctgtca ggatgaacac ttgttataac cgaatcattg atgggcgaat
    13921 gaaaatacta caggaggtat tgggatgctg gcaaaagaca taatgatacc gacacccttg
    13981 caatgtcatc aaaagatgac cctgacagaa gtgtccggga tgttgctgga gaatcatttg
    14041 gatggcctgc cggtagttga tgatgggggt gactttgtcg gttatttcgg tcgccgacaa
    14101 ttgtacaaag cccttcatca gcataaggat ccccagatgc ctgttcgtga attgatgatt
    14161 acgaatgtca tgacggtttc gccggatact gctttaaccg atttgtatcc tctgcacaat
    14221 accattatgc cagtgctcga aaaacgcaag attcttggcg taatcaccag ggaattagtg
    14281 gctatgtaca gtaatttgga ggcgcatggt tattcccccc aaaccatggc aacttttgac
    14341 gctgcctacg aaggtattat tatcatcaac aacgatctgc aagttttggc cttcaatcga
    14401 gcagcagaag agattctggg aatcaagcgc gaaaacattc tcaaccgacg ctatcccgaa
    14461 gtatttcccc gtggtaatct gcatcgggtg atcgaaagca ggcaggcaat tgccaatgag
    14521 aagtttgtcc atcaccaaag gacctattta tccatcaaca gtcctatcga actggcaggc
    14581 aacctgatcg gggccatcgc ggttatcaac gacatatcta cgctcgaaag tgtaactcat
    14641 gaactggaat acaccaaaaa attaaaatca gagttggatg ccattattga agcttcattt
    14701 gacagtattt ttgtaaccga tgccaaagcc aatgtgatca gcatcaatga agcctatacc
    14761 cggattaccg gcatcagtgc cgaagagatc atcggcaaaa acatgtatga tttggtcgaa
    14821 caaggtttgt atgaccgttc agctaccatc catgtcattg aaaaccgcac ccccatcacc
    14881 tttgcccaga atataaaaag cggcaaaacc ctcttggtaa ccggcaatcc ggtatttaat
    14941 gagcagggtg aactggcctg ggtcattacc aatggacgtg atataaccga gcttatgcgt
    15001 ctcaaacaag aggtcgaact ggctcagaac ctgagccggc attatgaaga agaattaaaa
    15061 aaagtggtgc aatcaggtga gatggtcgtt aattctccta aaaccaaaga gctcttggaa
    15121 ttggccatgc gtatcggtaa agtcgattcc accgtcttga tttccgggga atcaggggtg
    15181 ggcaaggaac tgattgcgag ggaattgcat aaaaacagca atcgcaaaga caagccttta
    15241 atcaatatca attgtgcagc tatccctgaa agtttattgg aatcagaatt gtttggttac
    15301 gaatcggggg ccttcagcgg agctcgcaaa ggtggcaaaa tgggaatatt tgaactggcc
    15361 aacgctggaa cgctatttct ggatgaaatc ggagaaatgc ctttgaccct tcaatccaag
    15421 ttgttgcggg ccatccagga aaaagaaatc ttacggattg gagccactac gcccataaaa
    15481 attgatgtcc ggctaatcac agcgaccaac cgcgatctgt gggagatgac ccggcgcggg
    15541 caatttcgtc aggatttgta ttatcgcctc aatgttgtgc cggtgtatgt gccgccgctg
    15601 cgggaaagga aagaggaaat tcccgcactg atttatcatt ttgttaatct ttttaacgag
    15661 cagtataacc tgaacaagaa aattgatgaa agaatgatca ccagtctgct ggaatatgaa
    15721 tggccgggga atgttcggga attaaagaat gccattgaaa gagcaatagt aaccagcccg
    15781 gaagaagtga ttcgaacgat tcaactgggc ggcaatgtca atggcggtga aatggagttt
    15841 aataccgaaa aaacagaaaa cagcggaatt gatttaaagg aaaaagtcaa tgcttatgaa
    15901 aaagacttgt tgcagcgtta tattcagaaa tacaaaagca gccgtaaagt tgcgaaagcg
    15961 ttaaatgtga gtcagacgac tgtagtgcgt aaagccgccc aatacgggat acctttggaa
    16021 ggttaatgtt gtgaaccgca gacagttcga tgcgaatcag ataagaatcg cgcaaacaag
    16081 aaaataaggc aaaaacacaa gcgtgtgaac cgaaattgca aaagtgaaaa ccgataaacg
    16141 aatgaaaata tgtctggcgt cacccggcaa gcccgtattt atgagggttg ttagaaaaat
    16201 gtcgcggaat ttgcagaagt tcgtttggtt ggcaaacatt ttgcattgta ttgaatatag
    16261 aaatgataca ccgttgccaa agataaagga ggaataagaa atggatttta aaatgaccga
    16321 agaatacgaa atgatcagac agtcagtcag agattttgca gaaggcgtcc tggcacccgg
    16381 tgtggttgat cgggatgaaa aagagtattt tgaccgcact ttgtttgata aaatgggaga
    16441 attgggtctg accgggattc catttgaaga aaaatatggc ggagcgggaa tggattatgt
    16501 tgcctatgcc attgccgttg aagaactggg gcgggtcgat gcttccgctg ccgacaccct
    16561 gtcttctcat ttaagccttg gaacctggcc gatttatgct tttggcaccg aagaacagaa
    16621 aatgaagtat cttgtccccc tggcggaagg aacaaaactg ggtgcctttg gtcttacgga
    16681 agctggagcc ggatccgatg ccgtggccat gacctgcaag gctgatttgg acggatacag
    16741 ctatgttctt aatggcagaa aaatctttat taccaacgcc gaggcggcgg atacctatat
    16801 cgtctttgcc cgtaccggac ccaaggaatt aagaagcaag gcgttcagcg cattcatcgt
    16861 ggaaaaagga acacccggtt tcagttttgg gaaatttgaa aagaaaatgg gcctcagagg
    16921 aacccaaaac tgtgatctgg tctttgagga ttgcagaatt ccgaaagaaa atctgctggg
    16981 ccaagaagga gaaggtttca aaatcgccat ggcgaccttg gacggtggca gaatcggcat
    17041 tgctgctcag tcggtagggg ttgcccaggg agcttttgaa atcgcccgtg attattccaa
    17101 acaaagaata caatttggta aaccgatatc tactctccag gccattcaat tcaagttggc
    17161 tgacatgatg gtggatatca aagcctcccg gcttttgact tatgatgccg cctggagaaa
    17221 gagccagcat atggaatatg gcgtggatgc tgccattgcc aagctgaaag cctctgaaac
    17281 agccaatcag gttgccaacc aggcggtcca gattcatggc ggctatggct acactcgcga
    17341 gtatccggtt gagagaatta tgcgtgatgc caagatcact gaattgtatg aaggtaccag
    17401 cgagattcag cgagtggtta tcagtaataa cgttctgcgt taaccgggag gtgtgaggca
    17461 tggattacag cgataaaata aaacactggg acgatttgca tattggtgat gctgccactt
    17521 ttaccaagac cattacggaa accgatgtga tattgtgggt tggattgacc ggcgacttga
    17581 atccgattca cattgacaag gagtacagta aaactacccg cttcggcgat gttatcgtcc
    17641 ccggcgtcat ggtgctgggc ctgatttcca ataccattac catggcgact tttggtaatg
    17701 tttatgccaa tcaaagcatt aaatttttaa agccggtcta tattggcgat accatcactg
    17761 ctactgcgac catcgttgaa aagctggaag ccaaacatat ggtcaaactc gaaaccaaat
    17821 gtgaaaacca gaagggcgaa ctgatcatgg ttggcgaagg catggaatat attttaagct
    17881 aaatatttat tcggcttgtt tgctgatgtg ccgttcctca tggtgatgcg acgcggcgaa
    17941 acgtcaggcc ggcaaaaacc ccgttattga agcggctcgt atggtggtga tacctttctt
    18001 aaagaagttg tccctcaaac gagcgtagct taatgaacgg gatttttgta tatacgagtg
    18061 aacccgggcg agtgaccgtt gttgactgat cattcacccg gtttttttga taacacaata
    18121 actttttaac atcctattaa taaggtttcg tgcccggacc gtatttagat gaatttacca
    18181 gaggttgtaa aaagttattt aatagcagga aaacggtaat ccatagagaa tggtataatg
    18241 tgcggaatta accgataatt cattatatat ggaaagttta aaaaattaga caaaggagga
    18301 gggaagtcct tatcggttac ggtgttcttc atccatttta attaacaaaa ggaggtttat
    18361 gatatgacaa gggaagtagt actggttggt gcttgcagaa ctccggttgg ttcgttcgga
    18421 ggagcattaa aagatgtaag tgtagttgaa ttgggcagtc ttgtgatgaa cgaagcgctg
    18481 caacgtgcag gcattgaagc ggcatgtttg gatgaagtta tttttggctg tgttctgcaa
    18541 gcagcccaag ggcagaatgt ggcacggcaa tctgctgtta atgcaggaat cccgattgat
    18601 ataaccgcca tgaccataaa taaagtgtgc ggctcagggt tgcgggcggt tagtttggct
    18661 gcccagatca tcaaagccgg ggatgcagac gttatcctgg cggggggcat ggagaatatg
    18721 tcagcggctc cctttgccct ggaaaaagcg cgttatggtt acaggatgaa taacggtacg
    18781 ctggttgacg tgatgataaa agacggttta tgggaagcgt ttaacaatta tcatatggga
    18841 atcacagccg agaatatcaa tgaacagtat ggcatcagtc gggaagagca ggatgccttt
    18901 gggttacgca gccaggaaag ggccttgcag gccatcgaaa caggaagatt caacgatgaa
    18961 atcgttccgg tcaccatcaa agggaaaaaa ggtgatcagg tatttgccat tgatgaattc
    19021 ccgagggcaa cgacagccca aaagctggca gggcttaaac cggcatttaa aaaagatggt
    19081 tcggtgactg ccggcaatgc ttctggtatt aatgacagtg cggcagcggt catcgttatg
    19141 tcaagggaaa aagcagatga gatgaacatc aaaccgatgg cccgggtgct gagttatgct
    19201 tccggcggag ttgatcccaa ggtgatggga ctggggccga ttcctgccac ccgaaaagcg
    19261 ctggccaaag ccggcctcac catcgatgac atagatttga ttgaggccaa tgaagctttt
    19321 gccgcccaat ccatcgcggt ggcaagagac ctgggttttg aaaatcatat ggacaaggtg
    19381 aatgtaaacg gaggagcaat tgccattggg catccgattg gtgcatccgg tgccaggata
    19441 ctggttacct tgttatatga aatgcaaaaa agagaagccc gcaagggtct ggctacgctt
    19501 tgcattggag gtggcatggg cacggcaatg gtcgttgaaa aatgttgata actgcgaaga
    19561 aggcctataa accatcgacc aaataatcat taatgagggg gaaacgtatt gccacactat
    19621 aattatcttt accaaacccg tgacatcaag tttcagatga aagaatggct cgacatggac
    19681 aaacttttat cctgtgatgg ttataaagac tactatggcg ctgacgatat tgatgccttc
    19741 cttgaggtaa atgccaaaat cagccgcgat gtgatctgtc cggcaaacaa ggattccgat
    19801 gaaatcggga tgaaatttgt ggggggagaa aagcaggcag taatcagtcc ggattcattt
    19861 aaaaccgtct acaataccgt agtggaagca gggttggggc ctcaatttgg tgaccgccaa
    19921 tcggaaggac gtattccttt gtcctggtac gctcccatct tggaacaaca atccggtgct
    19981 tccccggcca tggtgatgtt atggtgcctg acccagggag ccacgactgt actgcagttt
    20041 gagggtacgg aaaaacaaca acagcttttt ctgcccaaca tgtttgcggg taaatggggc
    20101 ggcaccatgt gcctgacaga accgggggca ggatctgaag ttggcaattg ttccacaaaa
    20161 gcgttcgcaa ccgataccag aggcttatat aagattaagg gcttgaagtg cttcattacc
    20221 tctggtgacc atgactgcgt tgaaaatttt gtgcatctgg tgctagccaa gaccgaaggt
    20281 gcgaaggcag gtacagcagg catttcgctt tttattgtgc ccaagttctg ggtcaacgat
    20341 gatggaacca tgggtgattg gaatgatgtc actacggttg gaatcgagca caagatggga
    20401 atccatggct cttccacctg caccttggcc tttggcgaaa acaatcactg cttgggatgg
    20461 ctggtcggag acgagttgcc agttgacggg cgcggcaaag gcatggcgca gatgtttcgg
    20521 tacatgaatg aagaacgcct taacacaggt ttgttttcgc tgggtgcctt cagttccgcc
    20581 tactatgctg cgcttgaata tgccaaggtg cgggtacagg gcagaaaatc caccgattca
    20641 aaaggatcgc aagtccggat catagaacac gaagatgtcc gccgcatgtt gttgtatcag
    20701 aaggcttgca tggaagccat acgagcactt atttatacga cttattatta tgttgatctg
    20761 tcccatgagg ccaacgatcc ggcagatagg gaatacgctg atgatatgtt tatgatcaat
    20821 aatccagtgt gcaaggcgta tgcatctgat atggcctgga gcctgaccca ggaagccatt
    20881 caggttcatg gcggttacgg ttttatggaa gaatatgcac cggcttcgct ggcacgtgac
    20941 tgcaaaatct attccttatg ggaagggacc aactttatcc aggcccagga ttttaccggt
    21001 cgcaagttta ccatgaagaa cggtgaacct tttaaaaaat gggtgggaga aatcgaggaa
    21061 tttgttgcca ggcaaaagat gccagaattt gcagccgaat ttgctttact ggaagaagcc
    21121 ttacaagcat ttaaagccat agttgccatg aatgacgact ggcaaaacac caacaagcag
    21181 cttaaacagc tgtttgctac gcggacactg catgcagcgg ctcgtgtcta ttgcggcaag
    21241 ctgatgcttg atcagggttt gttggcggcc agtcaattgg cccaattagg ggatgaacac
    21301 tatgatgcca atttttacaa gggcaagata gccagtgtca aattttatgt gctgaacgtt
    21361 gtacctgaag tctttggcta tgaaagggct atgaaggaaa gcgacaccag cgcaattgat
    21421 atcccggaag aagcatttat gtaatctgaa agcctgacag cagaaaagtg agattgcttt
    21481 gcgctcacat ttctgctgaa tttttctccc gaggggtata ttattgagcc caaaatacgc
    21541 aaaatagaat gataaatttt cgggtatcta cgattatata acactttaac tgccattata
    21601 tgctacaatt tattaaaatg cattgatcgg gaaggagcgg caacgggtgg cgatagcagt
    21661 atatccaggg agttttgacc ctgttaccat ggggcatatt gatattttgg aaaggaccag
    21721 ccggatattt gacaaaattg tagtagcagt ggtgcacaat gtgaccaaac aggctctctt
    21781 taccttggat gaacgggttg agctcattaa aaatagtgta cagcatttgc ccaatgtaga
    21841 ggtggaatgt tttaacggtc tgttggccga ttttctgaag tccaaggatg caacggtgat
    21901 tatcagggga ctgcggtcaa tcaccgattt tgaatatgaa tcccatatgt ccatgatgaa
    21961 taaaaacctg ttgccggata ttgatacagt ctttatcatg tccaatccct gttatatttt
    22021 tgtcagttcc agcggaataa aagaagcagc cctgctggga ggcagtgtat gcggcctggt
    22081 tcctaaagca gtggaaatag gactgaagga gaaactacat cgctttaaat agcaataaaa
    22141 agtccgcctc tattgatgtg agaggcggac tcttttttgt tcattaatcc aaaatagtaa
    22201 ctttgacggt cttcatacca tagctcaggg cttgttgatg agttgcaaag caaaggtcga
    22261 tgcgatttcc tttgatggca ttgccctggt cggcagcgat gccttcaccg tagccttcca
    22321 cataaagccg ggttcccatg ggaatcacgc gggggtcgac tgcaataatc cctctaccca
    22381 acggcaggcc gatataagaa ggggctccat aataaggata attgcatttg taacaggaac
    22441 aataagcagt tgctgtcatg gtcagcgttt ttgcgcgatt cacacttgag ccactgcgtg
    22501 aaggggtgga tgtgacgttg ctgttgtcac tcaaacccag tgccgttttg gttttggggc
    22561 cgacgatacc atcggtatcc aagccttggg ctttttgtaa tttaatgacc gctgcatacg
    22621 ttttggggcc aaaaacacca tccgcttctc cgcaccaata accttggtta ttaagagatt
    22681 tctgtaaagt cactacttcg gttccatggg atccccagga aagattggca gatgccatgg
    22741 ccttttcgct ttgtgtaaat aaaagaccgg taaacagtaa cgacaacatg ataacgggta
    22801 taatcaattt aattcgtttc atacaagact cctttccaga catcagcttg tgcatcagga
    22861 aatcaaacca gtcaatcaag ggtttgtttc cttgccatca accttgaacc cagtgtactt
    22921 gatttatggt gtgcggttca aaccggttta ggcgttataa ttatggacaa gtcgaagtct
    22981 gattggaagt cctgtgacat caggagggaa ttggacaaat cctccctgat gggattatat
    23041 tccgggccgg tcgtttcatt ttcctgtaat tgacgagcat taagaaaacg acaggaattt
    23101 gctgtcagct ataggtcgat ctgtttcaat ccggttttac agcgaattgc cagatcttga
    23161 tacagtttta ccgcaatccc gttatactgg gccagagtgc cgctcacttc cttaacagct
    23221 actgctgggt tgcctattgc caattgatgt gccggaataa tacggcccgg tgggaggaca
    23281 ctgcctgcgg ccaggaagct tccctctccc atctcacaac cagtggaaat gagggagccc
    23341 ataccaatga tcacccgatt taggatcgta cagggaccat gcacaatagc catatgtccg
    23401 accaatacat cctcgccgat ttttgcacag gtacccggtt cagcatgaag aactgcgttt
    23461 tcctgtatat tgcttccgtt gccgatttct atggtaccat aatcaccccg gagaattgcc
    23521 ccagccccga cataacattg attacctatc tttacgtcgc caatgactgt cgcctgggga
    23581 tgaacaaaac tgttttcgcc gatggtcggc cttttctctt cgaattcata aaatggcata
    23641 ttttaacccc ctatcagttt attgcatgac taattatgac gtagtaaatt gttctggtta
    23701 tttatatttt atcatagcag ttttagcaag gtggttgcag taaaatatgg gtcaacgttg
    23761 attttgctgc cattacgggt ataatgattg atatgacaat gaagggaatc aaattatgca
    23821 aaaccaggat tatttgataa aactgataga tgccggacaa aacgtcaggg tgtggttggc
    23881 gcgtaccaac cggctggtgg aagaggctca cattcgccat caaacatctg ctactgcgac
    23941 cgcagcgctg ggaagggtgc tgaccgctgc ggtgatgatg ggcagcgatc tgaaaagtga
    24001 ccaggatttg atcagtttgc gcttcaatgg caacggtccg gctgggacca tcattgccac
    24061 ggcagatggc aacggtcatg tcaaaggtct gatcggcaac ccacaagcgg atttgccgtc
    24121 ccgttcaccg ggtaagctgg cagttggtga tttggtgggg gcagaaggtt atcttgaggt
    24181 gcgcaaggac ctgggactga agcaaccttt tgaaggcaag gtgccactgg tcagcggaga
    24241 aattgctgaa gatctggccc agtattttat gcagtccgaa caaatccccg cgctggtttc
    24301 gctgggcgta ctggtaggtg cggatctgcg tgtcctggcc gcaggggggc tgattgtgca
    24361 ggctttgccc aatgctgagg atgctgtgct tgaaatcctg gaaaacaata ttttatcgct
    24421 ggggcagatc agccaacttg tgcttgaaaa tgaaacgctg gaagatattg cagcttctat
    24481 tatgcaggga attgaatata cgattattgc tcgcatgaat ctggcctttg catgcaattg
    24541 cagtcgggaa aggctggcga ttattctggc cgggctgaat gaggaggaaa tccgggaggc
    24601 agcggagaat aataaagaca ttgaagcggt atgcaatttc tgccggacga catatacctt
    24661 cagtcctgac gagatcaagg ctttaaaaag cgaaaagcct tagataaact atctaaggct
    24721 tgatcagatc aaaagcttta aagtgctctt tgaactttac cggaacgcaa acagcgggta
    24781 caaacataca ttcttctcgg tgttttatca tccagtactt tgacccgttg tacatttggt
    24841 ttccattgac ggttggttct tatatgggaa tggctatact ttttaccaaa ggttacgcct
    24901 tttccacaga tctcacactt agccactatt ttgccccctc tctatagcag ttgcacttag
    24961 atattttagc agaagcaaaa gcaaagggca agaagaagct ataaatatta tgtaggaaga
    25021 tgaatgaagt caggagggaa aacatgttta ctattaaaac caatgatctc ggtaatatta
    25081 cggtggaaaa agaagtagtg gagacgattg ccgggttggc cgccctggat tcctatgggc
    25141 tggtgggaat ggtgccccac aatctgcaaa gcgggattac ccatatgctt ggtttggaat
    25201 cgattcgcaa aggcgtggaa gtaaaaaatg gtacagatgg actgattgtg gatgtacatg
    25261 ttatcgtcgg ttatggtata aaaatatctg aagtagccct gagtgttatg cagaaggtaa
    25321 cttatgttct ggaaaaaaaa gccgggattc cggtggaaaa agttaatgtt aatgttgagg
    25381 ggatcagagt tatcaaagat aagtaatggg aggattaaca gtttgagttt gctgagtata
    25441 accggatatg atctggtgcg atgtataaaa gccggttgca tcagattgga acataatcgt
    25501 gatgaggtcg atcagttaaa tgtttttccg gtaccggacg gtgacaccgg aaccaatatg
    25561 tacttgaccc ttttatcggc tgtcagggaa ggcgaaaaaa acagcactgc atctttgggc
    25621 aaggtggcca aggcagtttc catggggtcc ctgatgggtg cccggggcaa ttccggggtg
    25681 atactatccc agattttcag aggttttgcc aaaacgctgg agaacaagga aaccgccaat
    25741 gcggttgatc ttgcacttgc tctgcgttcc ggagctgata ccgcctacaa ggcagtcatg
    25801 aagccggtgg aagggaccat attgacagta attcgcgaga ttgccagggc ttgtgaacag
    25861 gaggcccaat caggttctga tatactggcc gttctgctga tcggcgtgga agaaggctat
    25921 cgtacgctgg agaaaacccc ttccctgctg cccatcctca aggaagcagg agtcgtagac
    25981 gccggtggac gggggctgct gtttttcctt gaaggcgtga ttgaggggct ggcagctgac
    26041 aaggaaattc aactagacag ttacaaggaa aaaaccatta ccagtcgcca ggaaatgatc
    26101 aagcaagata tcgttttgga ttttcaatac tgtaccgaga tgctgatcaa ggggcaggat
    26161 ttaaatcctg aggatatcaa ggaccatttg cgtccgctgg gagattccat gctggtagtc
    26221 ggagaagagg agattgtcaa ggttcacatc cattccaatc atcccggcaa ggtgttggaa
    26281 acagcactgc aattcggaca gttgagtgat ataaaaatca acaatatgct ggaagaggta
    26341 catgaaaacc gtcatgattt tgaaccgaac aacctgtctg cgttttgtaa aaaaatcggc
    26401 ctggtagctg tagcagcggg tgaagggatt gtcagtatcc tccaaagctt gggggttgat
    26461 caggttgtgc agggtggtca gagcatgaac cccagcaccg aagatctgct gaaagcctgc
    26521 caggatgtgc cagcgcaagc tgtgatcatt atgcccaaca acaagaatat tatcatggcc
    26581 gccgaacagg tgaaggaatt gtctgacaaa caggtgacgg ttttaccgac ccgctcagtg
    26641 atgcaggcga ttgctgcgct gattgcctat gactctgagg gcgaattgga tgtcattatg
    26701 gcagccatgc aagcggaaac agaaaaagtc tcctatgccg aagtgactca tgcggtgagg
    26761 gattccgggg taagtggtct ggagattgaa gcgggcaacg tgattggctt gattgacggc
    26821 aaaatcagcc agaaagcaga cagtgaagaa gaagtggtct tggctatttt gcggaatatg
    26881 atcaagcccg acagcgaatt gattaccttc ctgtatggta atggtttgga agaagcagaa
    26941 gccagcgcgc ttcaggaaaa ggtgatggaa atttgcccgc agtgcgaagt ggaaatgcat
    27001 tttggcgggc agccccatta cagctatttc atatctgttg agtaatttgg gacttatgct
    27061 attatgggcg gtttttcacc gttttgaaac ggtgaaaggc cgtcttgtca tatcatgcgg
    27121 ggatgaggcc gggaggttgt tatggaaagt ctttttatgg aactgcaata catcaagggg
    27181 gtaggcccca aacgaaaacg tgacctgcaa aaattgggcg tggacaatat tttcgatttg
    27241 ctgtggttgg ttccgcgtag ctatttcaac cgttctcact taaccccgat ttgtgatttg
    27301 ccgcttgata cgaccgtgaa cctgcaggcc cgggttttgt ctacgaccag taccagaacc
    27361 gggcggggat tgcagatttt caaggcgctt ttggaagatg acagcgctgc gattcatgct
    27421 atttggttta atcagccgtt tttaaccaat gtaatcaaaa ccggccagca aatactggtt
    27481 accggcaagg tggacgggcg ttacaaggaa ttgcagttgc aggtctctgc ctatgaaatc
    27541 ctggaagacg acgaagttat ggcattcagc gtggtaccga tttatccgct gagcgggagt
    27601 ctgaaccaaa aaagtttgcg caagataatg aattatgtgc tgcaaaactt tctcaccgat
    27661 tatccggaga ttttggacgc tgatttgaga gagaagtatg aactgctgga tatccattct
    27721 gcgctgcacc atattcattt cccggagaac gcagaaaagt atctgcaggc cagaagacga
    27781 ctggctttgg aagagttatt gttgtttcag atatcacttg acaagcaccg cactgatcgt
    27841 cagccagctg ccccttgtgt cattcacctg gaaaaaacta atttggtaca gcatttgcgg
    27901 caaagtttgc cgttccggct gacggatgat caggaaaaag tcgtgggaga gatctatgcg
    27961 gatatgcaag ctccccggac gatgaaccgt ctgctgcaag gtgatgttgg cgccggcaaa
    28021 acagtggtgg cagcgctggc catggccaaa gcagcagcca gcggatacca ggctgcgctg
    28081 atggcaccta cggaaattct ggcacagcag cattttgctg cgctgcagag actttttgct
    28141 ggcgcgccgg tcgaaatggc ctgtctgacc ggcggcactt cccaggcaca gcgtcgtatc
    28201 atgttggagg cagtgcagcg tggagaaatt gatatcctgc tgggaaccca tgccttgatc
    28261 caggaacagg ttgaattcag gcgcctgggt ttggtgatta ttgatgagca gcatcgcttt
    28321 ggggtacgcc agcgggcccg gcttggtcag aaggggataa atccagacgt attggtcatg
    28381 acagctactc ccatcccacg aacactggct ttgactgcct atggggatct ggatctttcc
    28441 gttattcatc aactgccgcc cggacgcaag ccggttaaaa cccgctatct tccacgttca
    28501 tcccggtcaa aagcctatga ttttgcccgg ccaatgattc aacaagggat gcagtgctat
    28561 gtcgtctgtc cgctggtgga agaatcggaa aaacaggata tgcaggcagc cattacgctg
    28621 tatgaggaat tgcgcaccca tatttttgcg gaattctcag ttggtttgct tcatggccgg
    28681 atgaaagcca acgaaaaaga aatgattatg cagcgcttcc aggcaggcca gattcaggtg
    28741 ctggtatcga caacggtgat tgaagtgggc gtggatgtac ccaatgcttc cgtgatcatt
    28801 attgagcatg cggaacggtt tggtttgtcg caattacacc aactgcgggg cagagtcggc
    28861 cggggaagcc atcaatccta ctgtattctg ttggcagatg cacggaccga agaagccaaa
    28921 cgtcgcttgc aagcgatgga gcaaagcaat gatggtttcg aactggccaa tgaagacttg
    28981 gccatccgcg gacccggtga cttttggggg gtgcgtcagc atggtctgga ggaactgaaa
    29041 gttgccagtc tggtcaaaga tttggagctc atcagattga gtatggagct tgccggccaa
    29101 ttggaaatag cctatctgca aaccccgttt ctgcaacttt atatgcagct taaatttaaa
    29161 aaaaatcatg aaatagcccg caattagaga atggaaataa cgcctggaca tgattgacgc
    29221 cccattgatt gccaaaataa aatcatttaa tttgcaccat cctactactg cagacctatg
    29281 gtttgcagta ttttttttga tattggaaag ggggattgaa aaatgaaaaa aacgtccggt
    29341 caacaacagc caaagaagcc ttcaacgaat gatgccggta atcaggccat ggagaacctt
    29401 aaatatgaag ttgcacagga aatggggatt aaaccgaaaa caaaaaataa aaagtcttga
    29461 aaatacttct agcatattcg cagatgaagg ggaaaaacta agaccaacct aataaaagga
    29521 ggtgaagata aatggcaaga aacagtaaca gactgttggt tccccaagcg aaagcggcta
    29581 tggactcatt caagtatgaa gccgcccagc aggtaggagt taacttaaag cagggttaca
    29641 atggcgatct gacttctgct caggctggca gcatcggtgg tcagatggtc aagaaaatga
    29701 tcgagtctta tgaaactggg aaatccacta tgaaataatt gtacagctga ctaattatac
    29761 tacacgaaca agcggaatca tttatgatcc gcttgttttc atgtgatgct taatgcattt
    29821 gttttacgtt gcgtacagtt ccagctgttc ccggctgatc cccgggaaaa gagacgcagc
    29881 aacgcccatg gagataatac gggcagtgtt ttcaatgatt tcgtcaactt ctttgggagt
    29941 gacggccaga tcgccgccaa aataagaaag cgtgtcttta accgattggg taaccgtcat
    30001 atcatgaaaa acggtattgc tgacagaaca gaagttattg atggcttgct gggcgatgac
    30061 cgcggcattg acaatggttg gacagccgat ggcgatgact ggaattccca gttcttcttc
    30121 agtcagggca tgacgagcat tgcctacgcc gccgccaggc tggataccgg tgttggacat
    30181 ttggatggta ttgccgatcc ggtcaacatt ttgtgccgcc agagcatcca ccactaccaa
    30241 tacagctggt ttcacattgt cgaccacgcc tttgatgact tcatatgttt ccaatccggt
    30301 aatcccaagg actcccggag caatgccgga aaccggtcgc attccttcca ccagcgcttc
    30361 cggagcatgc tgaaaataat gccgggttat gggactgtat tcaataaact ttggtcccag
    30421 ggcgtcagcg gtcgcccgcc aattgcccag tcccaccaat aatacggtat ctttgggctg
    30481 taaacggttg ttcagcaaga cctccatgct tttattcatg gcggtaataa tttcatcttt
    30541 tacataggga tcattgattt ttaaaggggg agattcaatt gtaacatatg ttcccagtgg
    30601 tctgcccata accgcaaccg cctgagggtc tttgatggtt atggtggtga ttttgatttg
    30661 ctccattttt tcaacttgtt cttcgacacc ctggatctcg cgtccggttg ttccccggac
    30721 caaatcccgt gcttcaacgg ccagatccac attaagatta aaatgattta aaaaatcgtt
    30781 cacggaaaat tacctccttt gcgctttatt atcatcccaa gctggtaata ttattcaagg
    30841 taaacaaaat taaaatggcg acttgcgtcg ccattttggg gagaggagaa ttttgtaaag
    30901 ttgttgggga gggtttgttt accggttttt ttctttccgg tacttatatc attgccgata
    30961 ttagctataa tatacattaa taacaaaaga atttttaaaa aatacaaatt caccttttta
    31021 aaagaaggta attttatgct ggaagagaaa ttgacaaagg agaatagact tgcgagtgat
    31081 atctggctac gcgcgaggca aaaaacttaa ggctccgagt ggcttgcata cccgccccat
    31141 tactgacatg attaaggaag cgctgtttaa tgtatgggga ccgggtattg ttaacgccag
    31201 tattttggat ttgtttgcag gcagcggcag cgttggtatt gaggccttga gccgtggagc
    31261 agcacaagct gtgtttgttg acaatgataa aaacgctgtc aaagtgatgg ctgaaaacct
    31321 tcgaatttgt ggttttgaca acggttatga gctgtatcat aacgatgttt tcaaagctgt
    31381 aaatacgttt tcacgtaacc gtcgttgctt cgattatatc tatgtcgatc cgccttttac
    31441 cgaggacaaa atctttgatc agattatgat ggtcctgggg caagcagata tattggcccg
    31501 gcaaggggcc ttggtaatcc ggaccagaag gcagcgggaa ctggcgcctt cattcgggaa
    31561 gttgaaccaa tatcgaaaaa aaaactacgg agaatcaacg ctacactatt accgttatat
    31621 ggaggaggac caaaacgatg atggaaatat ttcggatctt ggatgaactg gaactgctgt
    31681 tgaaaggtag ccgtaaagtg cccctcaccg gcggcaaatc aattattgaa accagccgtt
    31741 ttttggacag agtcgatcgt attagagcga tcctgccgga agagttggag acggccaagc
    31801 taattatggc ggaaaaagaa agaattgtca gtgaggcttg tgctgaagcc gacaagtatc
    31861 tggaacattc aaaaagtcac gtcgcccaaa tggttgatga caacgagatt accagaagtg
    31921 cccgcggcgc tgctgaggaa atcattgctc aaagtgaaca gattgctgcc cagatccgcc
    31981 gggatgctga tgagtatgcc gatggtgttc tctctcacat ggagatcgtt ttgcgcaaag
    32041 ggttggaagc ggtgcttcaa ggccgggatg atatcagaga agggcttcgc agcaacgatt
    32101 attgatgctg cctgcctgtc gccatgcaca gcaaaacggc gattatgata aaagccgcca
    32161 gcagggaata gagtgaccag gtccaggcat caaatgcata taaaaccttg tgataaggga
    32221 tcgcctggac ctgaatgccg gccatgccgg tcagcaaggc caggcgataa gccagaaagg
    32281 ttatgctgca ggccattgcc atctgcagca aacgtgacag caaataaaaa gacagacgaa
    32341 tcgatgttcc agccattata ctcatcacct gggcgatgac tgagatgccg ctgaacgcaa
    32401 gtatggcgct caagaccagc aaaaccgcca gcatgttgcc aggaacaatg attgctgccg
    32461 ttttggcacc cagggtcatt tcaaaaatac ccatgcataa accgtaaacc tgggcataat
    32521 ccagaggaaa aatatttagc aaggaaaaga gcagtcctgc cataacctcc agcaaccccc
    32581 actggctcaa catatgggta agcacggaaa atacaacgat aaaacctcct accgccatca
    32641 cattgcccag actgtcgcga atggcatcac ccagcatacc ggcgaaaccg gaacgctctc
    32701 cgttgtcaaa gagacttgcc tggcaggcgg ctttccaacc agaacccccg ggagggatgg
    32761 gaggcgcaga tcggggtgct ttgaaccgca gcaaaatacc cagcaacaag ttggaaagat
    32821 attgggagaa ggccagcagg taaccggccg ccgggttgct caacaaaccg attcctaccg
    32881 ccccaagaat aaacagaggg ctggcattgt tggtgaaaac aaggagtctt tcagcttctt
    32941 ccgtgcttag ctgctgctga tcatacagtt ttctggtcag cactgccccc accggaaagc
    33001 cggaagtgaa tcccaaaacc accaccagtg cactgcaacc cggcaagcga aacaggggat
    33061 gcataaccgg ttccatcagt acccccataa aattaacaaa acgaattttt accagtaatt
    33121 cggctacaat aaaaaaaggc agcagggccg gaagaacgat tgaataccac agcaacaatc
    33181 cctgggaggc ggcctgtacg gttgcctgcg gatgaatgat cataaaaaat gtaccggtca
    33241 gtattataat gacagacaaa gggaagcgga cattgctcac tccatcagac tccgttcacg
    33301 ttttattcta tatatatgaa ggatgtaagg ttccttagaa taagggggca gttgcaaatg
    33361 gaaaacagca aaccggtcgt cggactggtg ctgggggccg gcagcgcacg cggactggcg
    33421 catattggtg tgctgcaggt tttccgagag aaagggatca aggttgattt cattgttggc
    33481 agcagcatgg gggcgatggt aggagcaatt tatgccagcg gtgctgacct ttacatgctg
    33541 gaaaaaatgt tggaatgtat gaatcccaac ctgatgattg atgtgcaggt accccgttat
    33601 ggttttgttg ccggcaagcg gatcgatgca tttttaacct tgctgaccaa gaacaagagc
    33661 tttgatcaat tggacccgcc ggtggtggct gttgcgactg atcttatcac cggagcaagg
    33721 gttgtcctgg atcaggggcc ggttagagaa gccgtacggg ccagtatctc cattcccggt
    33781 gttttcacac cggtgcgcaa ggaagggatg gttttggtgg atggagcagt gattgatcga
    33841 ttgccggtgg aagtagccaa agatcggggc gcggatatta ttattgcagt ggatgtcact
    33901 tttgggccgg ataaaaaagt gaccatcaag aataccctgg atgtcataat gacatcactg
    33961 gacatcatgc aaaagtatca ttttagcctc attggttccc aggccaacgt attaatacaa
    34021 ccagcggtgg gcaattttgc ttcccgggat ttcgaatgtt ccaaggacat cattgcactg
    34081 ggaagagccg cagctcttgc caaagtcgaa gaaatcaagc aactgacgga cagtttcaaa
    34141 tcctgactgg agaggtggta aaatcaagac cgcctgtgcg cacggccgga tcaggataca
    34201 acaaggaata aacatcggaa gcctgcctgt caagcgcgag catttccgtc agcatcggtt
    34261 ggttggtttg acgacaggct tttttaaaat cactgccgcg tgaaaaaatt ggcaaatctg
    34321 tttgggattt aatttcttgc aggattttct gtcccgatgg cgaaaatcct aaaacgtgca
    34381 gatattgtgg accacattcg tcaaagcgaa tgatttgcgc tttatcaagc tcccacaggc
    34441 aatatagaag aatacggttg atacgactct gggaaaaacg tttactcttg attttctgtt
    34501 tgagttctgc aaggttgacg gattgtaggg cagctttaac aatgcggttt tccaaacctt
    34561 cttcgacttc ataaatatct gccaattttg ctgcggagga gagacgcagt ttgagcagga
    34621 tactctggtg caggggttct gcttcaatag gtgcgcggcc gtgggaaatt tcttcggcca
    34681 gaatttgcag cgtataaggg gggacggaag aggcaattgc agccaggttc tgttctttgt
    34741 ccagagccgc acgaatggca ctggcactgg cccagtcgga gagtttgttg ctgtgatagc
    34801 cggttccctg acggggaaat gtaagcgcta tgatggaaga atggagttcg tttaacacac
    34861 gcaggtattc cagtgccagg atgttgttgg gttgcaacag caaatcgttt aattgggggt
    34921 tatcgattat cttctgcagg gtgagcgaac gagcccgcgc aaagctgtag ccccgggaca
    34981 gatgttgttt gagaatcatt tgatattcgg gactttcgtt gctgattaca gcagatattt
    35041 tctgcagttc attaagattg ccgctctcgc tgccaaaaac cagatgagta ataattccgg
    35101 tctgtgccaa cagttggatg gcaccgcggg caaaataata ggcactgcgg accgcgaaac
    35161 aaaccggcaa ttcaaggatc aggtctgcac ctgcgtgcag cgccatggca gctcgagacc
    35221 atttgctgca ccaggcagcc tcgccccgtt gcaggaaatt gccgctcatg acacaaacaa
    35281 tcggagcgtc ggggacagct tttttagccg cctgcaacaa atgcaagtga ccgttgtgaa
    35341 aaggattaaa ttcagcaatg ataccaagtg ccgtcatgta catactcctt ttgctttcgg
    35401 tccgcaattc acaaagcaat aaagctcttt tgataagtga tcgttctgcc tgctgcgttc
    35461 cataaggcgg actcgatttg tggtgacggg tttttcaaac cagggggtta cataaattta
    35521 ccacttataa attcatttat caaaggcttt atggtaaaat tttattcaaa gtattccaat
    35581 aacgaacggt atgaataggg aggatgaagg atgagtttat tagccgagat taaagagaaa
    35641 gcttccaaaa agggtaaaac agtcgttctg ccggaaggca cagaggaaag aacactgctg
    35701 gccgtcaaag ggatttgtga cggaaaaatc gccaacccga ttctgttagg caatgaaagc
    35761 gatgtaaaag ctgctgccag caaggccggt gctgatatct ccggagctgt cattattgac
    35821 ccggttaaag caccgtattt tgatgatttt gtccaggctt tttatgaaat gcgcaaaagc
    35881 aaaggggttg acatggacaa agccaaagcc accatgcagg atccgctttt ctttgcttcc
    35941 atgatggtac aaatgggtaa ggcggacggc gaagttgccg gcgcggaaaa caccaccggc
    36001 aacgtgttga gaccagcgtt gcagataatt aagactgctc ctggtatttc agcggtttcc
    36061 ggatctttta tcatgatcgt acccaattca gaatttggcg ataaaggcat ttttgttttt
    36121 gctgattgcg ccgtaacccc catgcctacc gctgctcaac tggctgaatt tgccaagaca
    36181 tctgcccaga cggcggtgaa tctgtgcggg atgaaagaac ccaaaatcgg tatgttgtct
    36241 ttctccacca aaggcagtgc tgatcacgaa gtggtcgaca aagtagtgga agctaccagg
    36301 attgccaaag agagattccc cgaactggcg gttgatggtg aattgcaagc cgatgcagcc
    36361 atggttccca aagttggctc ctctaaagcg cctggcagtt tggtggccgg acaatgtaac
    36421 gtactggtct tcccggatct tcaggccggc aatatcggtt ataaactggt gcagagactt
    36481 gccaaagctg aagccatcgg gccgattctc caagggatgg ccaaaccggt taatgatctt
    36541 tccagaggct gcagcgtgga agacgtcatc aataccgtag ccatggtcgt aatgcagtca
    36601 taaatcaatg aatccatgaa attaaaataa cgaggaggaa gaacattcat gaaggtatta
    36661 gtggtaaatg ccgggagttc gtcaatcaag tatcaggtat tcaatatgga agacgagtct
    36721 gtactggcca agggtctggt cgacagagta gggattccgg gaacgacatt ggaacacaag
    36781 ccaaccggca aggatgaagt tatcattaaa aaggatctgc ccgatcacac tgcagggatg
    36841 aaattggtgc tggaagttct ggccaacagt gaatacggtg tcatctctga catggctgaa
    36901 attggtgcag tcggccaccg ggtcgtacat ggcggcgagt cctttgccga ttccgtagta
    36961 atcgatgatg ccgttaaaaa ggtcatcaag gattgtttcg aaatcgctcc gctgcacaac
    37021 cccccgaatc tgatgggaat cgaagcttgc caggaattaa tgcccaatgt agcgcatgtg
    37081 gctgtttttg ataccgcttt tcatcagacc atgcaagccg aaaactatct gtatgcgctg
    37141 ccctatgaag tttatgagaa attcaaggtc cgcagatacg gtttccacgg gacatcccat
    37201 tactatgtag cccaccgggc tgcagacatg ttgggcaagc cttatgaaga ttgcaagatc
    37261 gttacccttc atctgggcaa tggcggcagt atggcagcca ttaaagacgg caaagtggtg
    37321 gatacttcca tgggctttac gccgctggaa ggattggtca tgggcacccg ctccggagat
    37381 atcgatcccg ccatcgtttg gttcctgatg gacaaactgg gtatggacac agctgaagcc
    37441 aacaactatt tcaataaaaa atcgggaatg ttgggactgt ccggcgtttc caatgatctg
    37501 cgggatgtgc tggaagcagc cggctcaggc aatgaaagag ccaaaatcgc tttacaggtc
    37561 tactacaatc aggtcaaagg ctacatcggc aactacatgg ccaaattaaa tggctgcgat
    37621 tgcctggtct ttaccgccgg cgtaggggag aacggctatg acgtacgcga ggctatctgt
    37681 gcagatatgg attatctggg cgtaaagatg gacaatgaaa aaaacaaggt aagaggcaag
    37741 gaagt
//
LOCUS       DAOTHZ010000011        37452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1197_length_37452_cov_7.236971, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000011 DAOTHZ010000000
VERSION     DAOTHZ010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 37452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37452
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1197_length_37452_cov_7.236971"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /gene="pstA"
                     /locus_tag="P5549_02555"
     CDS             complement(<1..785)
                     /gene="pstA"
                     /locus_tag="P5549_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRW11987.1"
                     /translation="MDVTKQKDKIIYALFWLSGLIIVAILVSIIGYVMVKGISSISLA
                     FLLEAPRRAGAQGGISTTIAGTVYLTVLALLIAVPIGVGAAVYLEEYAHSQSKFASIV
                     NLTAETLAGIPSIVFGLFGFVFLVIFLDLGWSLLSGSITVAIMILPTITRTAQEAILT
                     LPREYRENSLALGASKWQTIWKVILPFALPGIVTGIILSIGRAIGETAALILTAGSSL
                     GMPMSVFDPARTMSVHLYMLAMEGISMDRAFGTAFLIIILILI"
     gene            complement(772..1713)
                     /gene="pstC"
                     /locus_tag="P5549_02560"
     CDS             complement(772..1713)
                     /gene="pstC"
                     /locus_tag="P5549_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRW11988.1"
                     /translation="MEEALQNQKINILAPTNQLSANHSHSRRRRGEKAIEKILVLCAA
                     IFILAIFLITLFIFAKGMPLIAKIGLGDFLFNTQWSPTKGMYGIGSMFIGSVLITLGA
                     LIWTVPLGLSTAILMAEIAPPRIGTILGNLINLLAGIPSVVYGFIGLIVIVPFIRENL
                     GGNGMSLLAGAIILGIMILPTLINISRDSLLAVPREYKEGSLALGASHFQTISRVMVP
                     AAKSGIITAVVLGMGRAIGETMAVVMVTGNAAIIPGSILEPVRTMTSNIVLEMGYASG
                     DHQTALFATGMVLFVFIVILNLSINLFAKGGQGIGRN"
     gene            complement(1806..2657)
                     /locus_tag="P5549_02565"
     CDS             complement(1806..2657)
                     /locus_tag="P5549_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRW11989.1"
                     /translation="MKKNFGRIVLTGILVLALLVVTGCGKSNDAGEQGSDSGKLSGTI
                     TVAGSTSVQPFSEVLAEEFQAVNTGVTVNVQGGGSSQGVEAVISGAAQIGAASRDLKD
                     EEKARANLLVTPIALDGVAVVVNPANKVSALTAEDIKNIYIGNIKNWQELGGEDAPIT
                     IISREEGSGTRDAFSEIVLNKEDIVKDAIIQNSTGAVKTGVAGDKNAIGYISMAKVDQ
                     AVKALRVDGVEANEANVKNGSYKVQRPFIYITNGEPQGLSKAFIEYVLSDEGQSIISE
                     EGAFSIQ"
     gene            3152..3919
                     /locus_tag="P5549_02570"
     CDS             3152..3919
                     /locus_tag="P5549_02570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HRW11990.1"
                     /translation="MRLLVIQHVSAEGLGLFHDPLMARGWELDIRCMDMAGVSLPDNL
                     AGYQAFIILGGPMGAYEENIYPYLYKVEELVREAATNNLPTVGICLGGQIIARALGAE
                     VCPNPEKEIGWSHISILGEGKSSPLFLGLPDELPVFQWHGDTFSLPTGAVLLATSTVC
                     RNQAFLYNDHIWALQFHLEVNPTMIDHWSVLYENELSEFAGTDAAAVLSRDTRERWDS
                     MQTGREQFLANLLRLLQDQPFTPVPASEHSGQPANKE"
     gene            complement(3867..6005)
                     /gene="feoB"
                     /locus_tag="P5549_02575"
     CDS             complement(3867..6005)
                     /gene="feoB"
                     /locus_tag="P5549_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRW11991.1"
                     /translation="MNHRQMFSQPRDTDRTDCQEEALVVALVGNPNTGKSTVFNRLTG
                     LKQHTGNWPGKTVANARGSYLYQMQRFVLVDLPGTYSLAANSADEEVTRDFLCSGEAR
                     VAVVVVDATCLARNLHLVLQVLEMTEQVVLCVNLLDEAERKQIQLDLPALANILGVPV
                     IGTNARNGMGLDALKNAVWQVASGTLPTRPLRVKYGAAVEKGLALLIPAMEGMVEGKI
                     KCRWHALQLLDRAWEREEGAAQFPFLRMGDSQKLFPVLALTGQMLREEYTDDHSLRDH
                     IVTCIVARAEEIAQNVTRYGKEDYNSTDRRIDRILTSRAGGIPVMLVLLGIIFWITVK
                     GANFPSQWLAMGFFWGQERLTQIFLSAGWPEWLYGLLVLGMYRTLAWVVSVMLPPMAI
                     FFPLFTLLEDLGYLPRIAFNLDNYFKRACAHGKQALTMCMGFGCNAAGVIACRIIDSP
                     RERLIAIITNNFVPCNGRFPTLIALATVFIAGGLGGTGQSLVAVLSILAVIVLAVVIT
                     LVVSKLLSQTILQGMPSSFALELPPYRKPQIGRIIIRSLYDRTLFVLARALVVAAPAG
                     MMIWVFANINIGSLSILQHGANFLAPFAHLMGLDGFILMAFILGLPANEIVLPILAMS
                     YLSAGQMMEIISLQALRDLFVSNGWTWLTAVCTMLLCLNHFPCGTTLLTIRKETGSFK
                     WTLVTFAITTAAGIILCLLVAQSARWLGLV"
     gene            complement(6031..6267)
                     /locus_tag="P5549_02580"
     CDS             complement(6031..6267)
                     /locus_tag="P5549_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRW11992.1"
                     /translation="MDKLIPLHRLALGTCGKVSQLTANGNTRRRMLDLGFICGTRVQA
                     LQKSPSGDPVAYEIRGAVIALRADEASQILVEVF"
     gene            complement(6450..8573)
                     /gene="feoB"
                     /locus_tag="P5549_02585"
     CDS             complement(6450..8573)
                     /gene="feoB"
                     /locus_tag="P5549_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRW11993.1"
                     /translation="MEYKKLVIALAGNPNAGKTTIFNNITGSRQHVGNYPGVTVEKKE
                     GKITYQGYEITVIDLPGTYGLSGYSPDELVSRNVILKEKPDLIVNVVDASNLERNLYL
                     TSQLKELEVPLLLVLNMADVAEQSNKIDYTLLATLLGIPTVRAVGSKNEGTEEILQQA
                     LRSVENERQFPERVVRYRHEIEDELLYLRKQMGAAGQKKLEIAASTESGSAVALTYPS
                     RWLAVKLLENDREITQLIATVHPEMLPDILKSRERIQNLIGDDAETCLVEDRYAFVRG
                     VYHEACKAHAMDRLSVTEKIDSVLLNRALGIPIFLGIMWLLFQLTFALGAPLMDWIDV
                     GVSALAGWVLAQMPEGLLSSLLVDGIIGGVGGVLVFLPNIVLLFLGIALLESTGYMSR
                     AAFVMDGIMHRAGLHGKSFVPMLIGFGCSVPAIMATRTLENPRDRLVTMLVTPLMSCG
                     ARLPVYTLLIAAFFSPQAGGNVLFSLYLIGIILAIGMARIFRTYLLPGETEPFVMELP
                     IYRMPTLKYLLIHMWERTWLYLQKAGTLILAMSILMWGLFTFPLTNAQENQFADATQQ
                     LESSYAGKVGKAIEPLIKPLGFDWKTGVALVSGLGAKEIVVSTMGTLYSITDSDVLAE
                     EEDHAVQSFAQRVREQSGFTPLVAYVLMLFTLIYVPCLATIAVIKRETNGWKWPLFTV
                     GYTIVLAWVVSFVVYRAGLWLGIGI"
     gene            complement(8593..8814)
                     /locus_tag="P5549_02590"
     CDS             complement(8593..8814)
                     /locus_tag="P5549_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRW11994.1"
                     /translation="MRTLKDLRPGEKGRIKMVKGNGPIRRRIMDMGLVPGTELVMERY
                     APLGDPIEVKNHGFHLSLRKEEADTIILE"
     gene            complement(9101..9484)
                     /locus_tag="P5549_02595"
     CDS             complement(9101..9484)
                     /locus_tag="P5549_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRW11995.1"
                     /translation="MDNHLTPALEDYLEIILRLTEENGKATITEIASRLGIAKPSANQ
                     AIHHLRADGLVTQDRYGPVYLTETGRQKAEQVWHRHLIIRRYLQNVLKLSAAVAERDA
                     CMMEHVISTETLAAMLKTLDSKNKF"
     gene            complement(9626..11566)
                     /locus_tag="P5549_02600"
     CDS             complement(9626..11566)
                     /locus_tag="P5549_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW11996.1"
                     /translation="MINREQLQMCTLPELLNRAAMKFGERRCQWFKVAPGSTETVSLT
                     FAEVGMKVRDLTAGLLSMGIKKQDRAAIMAYNCPQWLWSDFAILSSGAVTVTIYPSFS
                     VREMAYIVNNSGSRFIFVKDQAGVDKVYSVLNDMPSLEKVIVMEDDCSLPDHAAFIHL
                     SQLQQLGRIYMHKNPYCYEKACDEIKLWDMASIIYTSGTTGDPKGAVHTHSSFMYAVN
                     ADNNMWTRNDCMLDEYDVLLSFLPLSHTYERQCGQMQSICLGETIAYAESPQTVVGDL
                     QLFKPTWFCSVPRIFERIYMALRDAASATPEGKAAFEKAMEIGIKAVDYVSDEEGFID
                     MGLDVNMADLLPEDLKKEYQWADQAVFSKARYILGPNYKMAFSASAGLPPNLCKSLMA
                     MGVRVCEGYGLTETMNAVNFSNLKAVLPGSMGPTNYYAEEKIAEDGELLVRGPMLFQG
                     YYNNPQATAEAFDEDGFFHTGDIAITVHNSKLGVDYYRIIDRKKSIMVLDTGKNVPRA
                     KVEGKFSTARYVEQICAVADERKYVAAIVVPKFEVVIPLLKAKGVAFDESKIVSVGEG
                     AAKVIVQVGDDLAGSPELRALIDEDIAEANKELENYESIKKYTISSRRFLEPLEEVTP
                     TLKLKPRVILKNFAAQVEEMYK"
     gene            complement(11674..13428)
                     /locus_tag="P5549_02605"
     CDS             complement(11674..13428)
                     /locus_tag="P5549_02605"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW11997.1"
                     /translation="MFKSFRGKLTGSYLLIILMLLLIGGAMTYFGFKDYYLHNLQQRL
                     TREAMLIADIIKYMQPESAATYHELCATAARDSAMRVSIIDQDGQVLGDSDYDASQLQ
                     THKSRPEVYQALHGQTGVAIRYSATEKVNMLYVAVPFAHQQQTGVVRLAMPLSDLAAI
                     NHKILMIMLLALFISAALALLLSLYMASRIAGPLVEITAATRDLSSGNLQRRISCYGD
                     DEMAYLASAFNDMSGNLEKGMIALNDMKNRFQTLLENTVNGIVLIDPGGIVSYANPAA
                     LKLLQIAEVMIGRSKTEVINHYELLEMISQVEAGWQPLHKELVLHQSGGKTVEVNIVP
                     IGGDERSHQGVLAVLNDISDLKRIDQIRKDFVANVSHELKTPLASISGFAETLMHTSG
                     EEQETVQEFSRIIYEEAQRLKTMINSLLELSRLEAEQSVVAFQPTDVDAVLRQALQVM
                     QHQSRTQMINLEYLAPAGKPMIESDPDLIMQVVNNLLDNAVKYSAPGGTVKLQLEESE
                     SHIIIKVIDSGVGIPEPEQQRIFERFYRVDKARSRKTGGSGLGLAIAKHILEKLDGSI
                     TVISKPGEGSVFAVCLPK"
     gene            complement(13421..14125)
                     /locus_tag="P5549_02610"
     CDS             complement(13421..14125)
                     /locus_tag="P5549_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW11998.1"
                     /translation="MDGKILVVDDEESLVRLIGYNLAKEGFTILAADDGNVAWQMIRE
                     EKPDLIVLDLMLPGKDGLEICRDLRREDNDTPILMLTAREEEIDKVLGLELGADDYMT
                     KPFSVRELNARVKAILRRNRGMTKPVPETKETRIGDFIIKADQYEIYHQGQRLDLTLK
                     EYELLEVLLRNHERVLKREYLLQLLWDYADGVNTRVLDVHISKLRDKIENDPKIPVYI
                     KTVRGVGYKFEVNDNV"
     gene            14497..16731
                     /gene="pflB"
                     /locus_tag="P5549_02615"
     CDS             14497..16731
                     /gene="pflB"
                     /locus_tag="P5549_02615"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HRW11999.1"
                     /translation="MENTAWEGFQKGAWNEEIDVQDFIQKNYTPYTDGDDFLSPPSQR
                     TLQLWEKVKALMQEEHCRQGVYSIDTKTVSSITSHPPGYLDRDLELIVGLQTDEPLKR
                     SIMPNGGIRMVRSAVDAYGYKLDPEIELIFTHYRKTHNQGVFDAYTQEIRQARKAGII
                     TGLPDAYGRGRIIGDYRRIALYGTDRLMEDRQKLMTGLERDNFTEELIRQREELTEQL
                     AALKELAEMAAAYGFDITRPARNAREACQFVYFGYLGSIKEQNGAAMSLGRVSTFLDI
                     YLQRDLQRGDINEEFAQELVDQFCMKLRLVRFLRTSEYNELFSGDPVWVTEAIGGMGV
                     DGRSLVTRSSFRFLHTLANLGPAPEPNMTVLWSANLPAPFKIFCTRTSIASSSIQYEN
                     DDLMRSYWGDDYGIACCVSAMRIGRQMQFFGARANLAKALLYAINGGMDELLGLQVAP
                     NIKPITADHLDFDELWERYEQVLAWLAKTYVNTMNIIHYMHDKYAYERLEFALHDLAL
                     HRTMAFGIAGLSVVADSLAAVQYARVKVIRNRQGLAIDYEVEGEYPAFGNNDPRADEL
                     AVKVQKTFLEELKKHHTYRNAQPTLSILTITSNVVYGKKTGSTPDGRKAGEPFAPGAN
                     PMHQRDKRGALAAMNSVARLPYRYALDGISYTFSIVPAGLGREETERPGKLSALLDGY
                     FAQGGHHINVNVLERATLEDAMQHPEKYPQLTIRVSGYAVNFIKLSKEQQLDVINRTI
                     HAAL"
     gene            16867..17604
                     /gene="pflA"
                     /locus_tag="P5549_02620"
     CDS             16867..17604
                     /gene="pflA"
                     /locus_tag="P5549_02620"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRW12000.1"
                     /translation="MLHEAYLHSIETMGMLDGPGIRYVLFMQGCPLRCAFCHNPDTWH
                     MSKGQVKSLAWLLTDIKDYLPYFRASGGGVTVSGGEPLLQTDFLEVLFAELKKLDVHR
                     AIDTSGFAALEKVKFLMGVTDLVMLSIKHPDPDKHREICGQSPQRPLALARYLTSIKL
                     PVWIRYVIIPGISNLPADLARLADLIKSMPNVERLELLPYHTLGRPKWEQLGLSTPLL
                     TVPPPLPEEMEAARQTMAGLLPGLKIV"
     gene            complement(17914..19587)
                     /locus_tag="P5549_02625"
     CDS             complement(17914..19587)
                     /locus_tag="P5549_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRW12001.1"
                     /translation="MSKLSAKTNQINVLVKGLGNITAAAGISLEALIAGLQLSHELPV
                     VAGIVNNRLRDLNFLLDRDCEVEMVELYSEAGLKIYRRSASFLLLKACKDLFPERRLM
                     IKHTLSNGTYCEFLDETINNVEVEQIARRMQEIVEADLPIQRKRLPKQQAQELLARQG
                     KPDTVKILAHRDKEEVHIYELDGFYEYFYGYMVSHTGVLNKFKLIYYPPGLVLQTPEP
                     NDPDLSQPFHEQGKLFQIYQEAKAWAGMLNTSHLGALNDIIENGKIDDLIRVNEALQE
                     KKIAQIADMIVHNPGVRVVLIAGPSSSGKTTFAQRLLIQLRVNGCRPVSISLDNYFID
                     QKLTPLDENGEYDFEAIEALKLDLFNEHLTRLVEGEEIEMPIYDFKQGCCLEKGIMLS
                     VPAGEPIIIEGIHGLNDQLTWSIPREQKFKIYVSALTQLNIDYANRIPTTDCRLLRRI
                     IRDSRTRGYSALNTIKRWPSVRRGEEKHIFPFQENADIMFNSSLVYELAALKPLAEPL
                     LKEISADHREYTEARRLLKFLSYFRSIVPDPIPPNSILREFVGGSWFDV"
     gene            complement(19698..21752)
                     /gene="pheT"
                     /locus_tag="P5549_02630"
     CDS             complement(19698..21752)
                     /gene="pheT"
                     /locus_tag="P5549_02630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRW12002.1"
                     /translation="MAVSINWLKQYVDIPWSPQELAHRLTMAGIAIEGVDEINGDSVL
                     ELDLTPNRGDCLGMIHLAREVAALQHQEIKIPPLKIRENGEKIDDYIKVDIADADLCL
                     RYAARVIKNVKIKPSPEWLQTCLVNSGIRPINNVVDITNFVMLETNQPLHAFDYDLLS
                     QAKRILVRRAEAEEKIVTLDDVERMLDKEMLLITDDGRPVALAGIMGGQNTEISDDTV
                     NVLLESACFYGVGIRKTSRKLALRSDSSMRFEKGADVNGLIYALNRAADLIQQLAEGE
                     VVQGICDCYPAPQTLRTVGLRPRRINALLGINLTTEEIKGYIHDLGFSLEEKDGQLMV
                     EVPSYRPDIELEADLIEEVARLHGYEHIPATFPGAASRGGLSPYQHFREKIQNLMART
                     FYEVINYSFISPRYFDLLGLAEDNRLRHTVQVANPLSEEQSVMRTMLLPGLLETVSRN
                     LARKNSNLAFFELGKVYFPEKGEQLPREELKLGAVVAGRLESNWLKQNVEMDFFYLKG
                     ILENFLSQLGIENTQFKDVVLDAYHPGRCARILCQGQELGVIGEVHPLVRQNFDLKPR
                     VCAFELDVDRLFDLSSPKHMMESITRYPAVERDIALLLREEIQSDQIVEVIKNSGGPL
                     LQKVSLFDVYAGDRLEAGHKSLAYRMVFQSGERTLTEAEVTQVMEAIVHAIKDKLDVR
                     LR"
     gene            complement(21765..22787)
                     /gene="pheS"
                     /locus_tag="P5549_02635"
     CDS             complement(21765..22787)
                     /gene="pheS"
                     /locus_tag="P5549_02635"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRW12003.1"
                     /translation="MIDRLLQLRQATEEAVKKVSDIEALNEIKVKVLGRKGELTNLLR
                     GLKDLSGDARVEAGKTANEIKAILENLIEARTSDIRARELERTLLAEKIDVSLPGLPL
                     QTGGLHPISQVIREIQMIFIGMGFTVAEGPEIESDYYNFAALNVPKDHPARDMQDTFY
                     ITEDILLRTHTSPCQVRTMEKMAPQLPVKIVVPGKVYRKDDDATHSPMFNQIEGLVVD
                     QGITFGDLKGTLMAFAKQVFGEDVRVRYRPSFFPFTEPSAEMDISCVICKGKGCRVCS
                     HTGWLEILGAGMVHPRVLLNGGYDPEKVSGFAFGMGIERVAMLKHGINDLRLFFDNDK
                     RFLTQF"
     misc_binding    complement(22838..23061)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23235..24005)
                     /locus_tag="P5549_02640"
     CDS             complement(23235..24005)
                     /locus_tag="P5549_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRW12004.1"
                     /translation="MKFISSSENQVIKLARSLHLKKYRDQENLFLAEGRRLVEEALAL
                     PDRIVALFIREELAGEFADRQDFACYVIDDKLLKHISMTETPQGMAAILRKPDWNLTA
                     LTAADSFLVLLNHLADPGNLGSIMRSCWALEVDGLLLTPDCVDPFNPKVVRSTMGAIF
                     HLPLIEAVSDRQLDDLQAHGFELIATGLENAMDYYDIDLRGKKILVLGSEASGIDPAL
                     QRRCSQTARIPINPSVDSLNVAAACAIITAEAWRQRRT"
     gene            complement(24120..24473)
                     /gene="rplT"
                     /locus_tag="P5549_02645"
     CDS             complement(24120..24473)
                     /gene="rplT"
                     /locus_tag="P5549_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRW12005.1"
                     /translation="MARVKRGVTTHRRHKKILKLAKGYVGSKSKLYRVANQQVMKSGN
                     YAYAHRKLKKRDFRQLWIARINAAARDNGTTYSRMVHGLKVAGVDINRKVLADLAISD
                     PQGFADLAELAKKSV"
     gene            complement(24493..24690)
                     /gene="rpmI"
                     /locus_tag="P5549_02650"
     CDS             complement(24493..24690)
                     /gene="rpmI"
                     /locus_tag="P5549_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRW12006.1"
                     /translation="MPKMKSHRAAAKRFKKTGSGKIKRSKAYASHLLGSKSPKRKRNL
                     RKAGLVSTVEEKRISKLIPYK"
     gene            complement(24706..25227)
                     /gene="infC"
                     /locus_tag="P5549_02655"
     CDS             complement(24706..25227)
                     /gene="infC"
                     /locus_tag="P5549_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRW12007.1"
                     /translation="MSKEWRVNEDIRVKEVRLVSEDGEQLGIVPIREALQIALDKGAD
                     LVEVAPSAKPPVCRIMDYGKFKFEQSKRDKDARKKQKVITIKEVKMRPNIEDHDFDVK
                     TKNARKFLTSGDKVKFTIMFRGREITHPELGMKLCQKFAADLADISTIEKMPKVEGKN
                     MVMILLPKIENND"
     gene            complement(25602..26600)
                     /locus_tag="P5549_02660"
                     /pseudo
     CDS             complement(25602..26600)
                     /locus_tag="P5549_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
     gene            complement(26931..27365)
                     /locus_tag="P5549_02665"
                     /pseudo
     CDS             complement(26931..27365)
                     /locus_tag="P5549_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
     gene            complement(27475..27789)
                     /locus_tag="P5549_02670"
     CDS             complement(27475..27789)
                     /locus_tag="P5549_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRW12008.1"
                     /translation="MANFTIAEFMEMLEPLIASPYMGVVVVDRNGFITDMNQTYLKLL
                     ETSPEEVMGQYILDVLPSSELADKWQKYRHHASSHRQRRTNHRCHGQKYFFGYVRSAV
                     AH"
     gene            complement(28016..29926)
                     /gene="thrS"
                     /locus_tag="P5549_02675"
     CDS             complement(28016..29926)
                     /gene="thrS"
                     /locus_tag="P5549_02675"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRW12009.1"
                     /translation="MLTITLQDGSRREFSEGSTFLEMAEKISAKLAKNVIAAEFNGQV
                     YDLTRPVNEDGALRLLDFKDEKARDVYRHSSSHVMAQAIKRLWPDTRLAIGPAIDKGF
                     YYDFDTEHTFTPEDFAKIEKEMADIIKSDYPIVRKELPRNEAIAYFRSIGEIYKVELI
                     EDLPEDAIISFYEQGDFVDLCAGPHVPSTGRLKAIKLMSLAGAYWRGNENNKMLQRIY
                     ATSFPKQAQLDDYLHKLEEAKKRDHRKLGKELGLFVLLDEGPGFPFFLPKGMVLRNEL
                     EKFWREQHEIAGYQEIRTPIMLNRELWERSGHWDKYKDNMYFTEIDETDFALKPMNCP
                     GGILVYKQNLHSYRDLPLRIGEMGLVHRHEMSGVLHGLMRVRAFTQDDAHIFMLPEQI
                     IAEIKGVMNLVDLFYSQFGFAYHVELSTRPENSIGSQEAWDLATDALRQALDERNIAY
                     KVNEGDGAFYGPKIDFHLQDSLDRTWQCGTIQLDFQMPERFDLTYVGEDGEKHRPVMI
                     HRVIYGSMERFIGILTEHYAGAFPTWLAPVQVRVLPITDRQHEYARNVLTALKAGGIR
                     AEIDDRSEKIGYKIREGQLQKIPYLLVLGDKEIEMDGVAVRHRKQGDLGSQKLDDFIA
                     TIKDEIANRHNI"
     gene            complement(29949..30542)
                     /locus_tag="P5549_02680"
     CDS             complement(29949..30542)
                     /locus_tag="P5549_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HRW12010.1"
                     /translation="MVIKIATTLLISTFIGYVTNVVAVKMLFWPRQPINLGFYKVQGI
                     LPKRQAQIAVSLGELVEKELLSIDDLLVQINTPANQQTIVTKISALVRERLNELLPRI
                     IPGRLIQLIIDTLDKILSQESAQIVEQLFKSGSEFLTHEIDISKIVEEKVNAFDLQQL
                     EEMIKNISAPELTFIKVLGGVMGFMIGLVQVIILLAG"
     gene            complement(30880..>31810)
                     /locus_tag="P5549_02685"
     CDS             complement(30880..>31810)
                     /locus_tag="P5549_02685"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HRW12011.1"
                     /translation="MIYKMQIATLAEENLLWDDFSHRFGWLRERGFVLQINREVCPNS
                     NTLLLDLVIEGDNNDLFMKDEDIVYIFKHQISEFLAEHILHDWENKMVRREISKRTRH
                     LNGEELKIVWHKAMDFLQRCNTNDSINLLFNYGRKNKISHKIMDHIYHYDKLVIEGLI
                     NFGMQDYWNELRFAVDLACEELNNEKEYNDFVRLLRYFVDTQTPRMPEVNLRMDQDGL
                     FFLWDGNGADIDEKIINYYSNELLYNDISLDDILVSILITISPRRIILHNSNSDNSEP
                     VKVIRQVFGERIKDCGGCERCRQHQEGERNKSN"
     assembly_gap    31811..31910
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(32210..32284)
                     /locus_tag="P5549_02690"
     tRNA            complement(32210..32284)
                     /locus_tag="P5549_02690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32250..32252),aa:Val,seq:gac)
     gene            32454..33194
                     /locus_tag="P5549_02695"
     CDS             32454..33194
                     /locus_tag="P5549_02695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRW12012.1"
                     /translation="MRQKKIIGYGFMFSGLILVSLLVGFYKFALHSNMEYWKKNHLLI
                     DRVKIAEKVVALTFDDGPDPATTPAVLTSLAKHDVPATFFVVGQHAEAHPALLQKISA
                     AGHELGNHSYSHRDFNHASGAEIRDEIRRTNQIIHQISGQQPVLFRPPGGYLSNEMIR
                     ITQEEKVIVAYWSWETDSKDWRSSNSQSIADHITGHIAPGQIIILHDGGKNGLTTARA
                     VDLMIKVLQQKGYRFVTMGELIKMGNQE"
     gene            33246..33992
                     /gene="pdaB"
                     /locus_tag="P5549_02700"
     CDS             33246..33992
                     /gene="pdaB"
                     /locus_tag="P5549_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family sporulation
                     protein PdaB"
                     /protein_id="HRW12013.1"
                     /translation="MPKVIGFWHLKNSTKLIIISILATLIMTTSVLYVQAYHKLKPIY
                     QIDTEQKVVALTFDISWGDKTPLPVIEILKANQVKSTFFLSGPWVKQYPEIPKRIQKD
                     GHEIASHGYRHINLSNLSKNEIKEEIMQAHQNIREVTGINANLIRTPNGDYNDQVIEA
                     AHECKYEAIQWSVDSLDWMNPGVNTITERVGKKVHPGAIILMHASDTCKQTTEALPQV
                     IKNLKSQGYELVTVSELMKLQKQERRDKQN"
     gene            34334..34552
                     /locus_tag="P5549_02705"
     CDS             34334..34552
                     /locus_tag="P5549_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12014.1"
                     /translation="MLSSDGAWARFIFSICFFLLSGSEMITGWLATLSGVAGTIELAT
                     ALTRYSPLQNLLQVLTASTPGQGARRDH"
     gene            complement(34546..34971)
                     /locus_tag="P5549_02710"
     CDS             complement(34546..34971)
                     /locus_tag="P5549_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12015.1"
                     /translation="MNFRRVFYIIALALAAYFLYAHNQTETVRETPAISQQEIFADFA
                     DLADNDIPKATLGGTFFTTDVFFPADFLGEAGDEFYVTMEDGHTQYTQRYIIATEVQK
                     DLSVKLVYQPKANWEGFRPPDGKYESYQFDGEKWTQTNQ"
     gene            complement(34990..35907)
                     /locus_tag="P5549_02715"
     CDS             complement(34990..35907)
                     /locus_tag="P5549_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12016.1"
                     /translation="MIIDLDPKIKFIRPQGKAMFPYCNSIYIDDEIKTLIDAGSGGNA
                     YQEIPREEIGLILLSHSHFDHTHGLGYFPQAKIMAGTEEQYAFQDENKFYITAGFQRW
                     EELMGSKKDEKMTMQTPLPDDVLVKPGFRQVELGGVFKDGDEIVVGSTRLLALHTPGH
                     SVGHYGFYFPEKKILFSGDMDISPRGPWYGGYDSDFGEVVASVQKLIALQPEVLVTSH
                     RKVFYDEIEKHLRAYVQIALDRDEKIMDFLAQPRSLQEIAEQELGFYELGRTAFNVFW
                     SKVMILKHLEYHIRAGEVEDLGNHLYKRK"
     gene            complement(35993..36403)
                     /locus_tag="P5549_02720"
     CDS             complement(35993..36403)
                     /locus_tag="P5549_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12017.1"
                     /translation="MGVKNKWSKGDPVNLSDLLKITPVANEILHLSNELNMSITDVIW
                     IASQILDNSEELKDSSIFKLEDEDIIPLEDDWEDIDLESLEDDLEQAPYMISVKIQSG
                     GRFPEVGFKAGINEKEDMNVFFDMIMEMMDRLDG"
     gene            complement(36904..37191)
                     /locus_tag="P5549_02725"
     CDS             complement(36904..37191)
                     /locus_tag="P5549_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12018.1"
                     /translation="MQLVSFNGLHMTDYNMATGKGVFAADNGQHEAELFFYLQGQDCL
                     GIRLGRHDQGISTQALEDYLAMHKTEIRRQIQPVVEELREEGRQQLMQIAM"
BASE COUNT         9328 a         9781 c         8481 g         9762 t
ORIGIN      
        1 atgatcagaa tcagaatgat gatcaagaaa gcggtcccaa aggctctatc catggatata
       61 ccctccatgg ccaacatgta cagatggacc gacatggtgc gggccggatc aaagactgac
      121 atcggcattc ccagcgaact gccggccgtc aggatcaggg ctgcggtttc accaatagcg
      181 cggccgatcg ataaaatgat gccggtgacg atgcccggta aggcaaaggg caaaatcact
      241 ttccaaatcg tctgccattt gctggccccc agcgccagac tgttttcacg atattcacgc
      301 ggcagagtca ggatcgcttc ctgggcagtt cgggtaatcg tcggcaaaat catgatcgcc
      361 acagtgatgc tcccggacaa caatgaccag cccaaatcga gaaaaatgac caggaagacg
      421 aagccgaaca gtccaaacac aatggaagga atgccggcca gggtttcggc ggtgagattg
      481 acgatagaag cgaatttgct ctgactgtga gcatattctt ccagatagac ggctgcgccc
      541 accccgatcg gtacagcgat aagcaaagcc agtacggtaa gatagactgt ccctgcaatg
      601 gtggtggata tccctccctg ggcgccggcg cgacggggcg cctccaataa gaacgcaagg
      661 ctgatggagg aaatgccttt caccatcacg taaccaatga tactgaccaa aatcgctacg
      721 ataatgagtc ccgacagcca gaacaaagca tagatgattt tatctttttg cttagttacg
      781 tccaatgccc tgtcctcctt tggcgaataa attgatactt aaattcagaa tgacgataaa
      841 aacaaacaat accatccctg tagcaaacaa ggcagtctga tgatcaccgc tggcatagcc
      901 catctccaga acaatattgc tggtcatggt acgcaccggt tcaaggatgc tgccggggat
      961 gatcgccgca ttgccggtta ccataaccac agccatggtt tctccgatgg ctctgcccat
     1021 tcccaatacg accgcggtaa taattcctga tttggctgcc ggcaccatga ctctgctgat
     1081 ggtttgaaaa tgcgacgctc ccagcgccaa cgatccttct ttatattccc gcggcacggc
     1141 cagcagtgaa tcacgggaaa tattgatgag ggtgggcaag atcataattc ccaaaataat
     1201 cgctccggcc agcaaactca taccgttgcc gcccaaattt tcacgaataa agggaacaat
     1261 cacaatcaag ccgataaatc cgtaaacaac cgaaggtatg cctgccagca gattgatcaa
     1321 attgcccagg atcgtgccga ttcgcggcgg cgcaatctcc gccatcagga tggccgtgct
     1381 cagccccagc ggtactgtcc agattaaagc ccccaaggta atcaaaacgg aaccgataaa
     1441 catgctgcca ataccataca tccccttggt cggactccat tgggtgttaa acaagaaatc
     1501 tcccagcccg attttggcaa tcagcggcat tcctttggca aagataaaca aagtgatgag
     1561 aaatatggcc agaataaata tggcggcaca gagcaccagt attttttcga tggctttttc
     1621 cccacggcgt cgcctgctgt gggaatgatt tgcgcttagc tggttggttg gagccaatat
     1681 attaattttt tggttttgca atgcttcttc catcgcttta tctcactttc attcaggatc
     1741 attgccaaag ggagacaact tgtggttgtc tccggcggca ggttgtgaat attcaacctg
     1801 cctggctatt gaatcgaaaa cgcaccctct tcactgatga tactttgtcc ttcatcgctg
     1861 agaacatatt ctataaaagc tttgcttaat ccctgcggtt caccgttggt gatgtaaata
     1921 aacggtcttt gcactttgta gctgccgttt tttacattgg cttcattggc ttccacacca
     1981 tctacacgca aagctttaac agcttgatca accttggcca tggaaatata tccgatggca
     2041 ttcttgtcgc cagccacgcc ggtttttact gctcccgtgg aattctggat gatggcatct
     2101 ttcacgatat cttctttgtt taaaacgatc tcactaaaag cgtctctggt acccgaacct
     2161 tcttcccggg aaatgatggt tatgggagca tcttctccgc ccagttcctg ccagttttta
     2221 atgttgccaa tgtaaatatt cttgatatct tcggcggtaa gtgcgctgac tttgttggcc
     2281 ggattcacaa cgacggctac accgtccagc gcaatcgggg taaccaacag attggccctg
     2341 gctttttcct catccttgag atcgcgggat gctgctccga tttgggctgc tccggagata
     2401 acagcctcta cgccctgact ggaaccgccc ccctgcacat tgactgtcac accggtgttg
     2461 acagcttgaa actcttccgc cagcacttca ctaaacggct gcacggaagt ggacccggct
     2521 acggtaatcg ttccggacag tttcccgctg tcgctgcctt gctctcctgc gtcattgctc
     2581 ttgccgcagc cggtcacaac cagcagcgcc agtaccagaa tcccggttaa aacaatcctg
     2641 ccaaaattct ttttcatctc aataatcctc cctttgttac atcgattttt ttcttcattc
     2701 acagcatatc aaccgattgt taactgtcat catggggagt attaaaatca actgcagcaa
     2761 ttgtaaattt tgtgtaaaag cgttcggcag cttatcatta ataccgcatc acccagaaaa
     2821 taaaaacggc cagttgcaag taagcactca tcaagcgttg catccaattg tgtcgccatt
     2881 ctacccgtgc cacagcagta gaagcagcaa aatctgcctt gcagtctctt gccagcccgg
     2941 cgcttggagc gcgattttga gtatacatga tacatcggcg gcggactgta gtaggcggta
     3001 gcttatggag tataatcagg atacagctag gcaaaccaag cccagaccca aactaagcaa
     3061 agtgtccggg taatgcagag gttctatcat ttttgtccgg attctcgcat cgttaccctg
     3121 gatttgtttc gatagaagga ggatatatca gatgcgatta ctggtgatac agcatgtatc
     3181 ggcggaaggc cttggattat tccatgatcc cctcatggcg cgcggctggg aactggacat
     3241 ccgttgtatg gacatggctg gcgtcagctt gccggacaat cttgccggct atcaggcatt
     3301 tatcatcctg ggagggccca tgggtgctta tgaggaaaac atctaccctt atctttataa
     3361 agttgaggaa ctggtccgcg aagctgccac gaataatctt cccaccgtgg gcatttgtct
     3421 gggtggacaa atcattgcgc gcgccttggg agccgaggtt tgtcccaacc cggaaaaaga
     3481 aatcggctgg tcacacatct ctattcttgg tgaaggaaaa tccagtccgc tgttcttggg
     3541 gctgccggac gagttaccgg tttttcaatg gcacggggat accttctccc tgccaacggg
     3601 agcagtcctt ctggctacca gcacagtatg ccgcaatcag gcctttttgt acaacgatca
     3661 tatctgggcg ctgcagttcc atctggaagt aaacccaaca atgattgatc actggtcagt
     3721 attgtatgaa aatgaattaa gtgaatttgc cgggaccgat gccgcggccg tactttcaag
     3781 ggatacccgg gaacgctggg atagcatgca aacagggcgg gaacagtttt tggcaaacct
     3841 tctccggctc ctgcaagacc aaccatttac accagtccca gccagcgagc actctgggca
     3901 accagcaaac aaagaataat tccggcagcg gttgttattg caaaggtcac cagcgtccat
     3961 ttgaaactgc cggtttcctt gcggatggtc aacagggttg tgccgcaggg aaaatggttc
     4021 agacacagca gcatcgtaca tactgccgtc agccaggtcc acccgttgct gacaaagaga
     4081 tcccgcaagg cttgcaaact gatgatttcc atcatctgac cagcggaaag atagctcatc
     4141 gccagaatgg gaagaacgat ctcgttggca ggcaaaccca gaataaaagc catgagaata
     4201 aatccatcaa gccccattaa atgggcaaag ggcgccagaa aattggcccc gtgctgtaaa
     4261 atgctgagag agccaatatt tatattggca aaaacccaaa tcatcatgcc cgccggtgcg
     4321 gccaccacca aggcccgggc cagcacgaac agagtccgat catagagtga acggataata
     4381 attctgccga tctgtggttt gcggtaagga ggaagttcca gggcaaaaga ggaaggcatg
     4441 ccttgcaata tcgtctgcga caatagtttg gataccacaa gggtaatcac cactgccaaa
     4501 acaatgaccg ccaggatact cagcacagcc accaatgatt ggccggtgcc tcccagtccg
     4561 ccggcgatga aaacggtagc caaagcaatt aaggtgggaa agcgcccgtt gcagggtaca
     4621 aaattgttgg taataatagc aataagtcgt tcccggggcg aatcaataat gcggcaggca
     4681 attaccccag ccgcattgca tccgaatccc atacacatgg tgagggcctg tttaccgtgc
     4741 gcacaggcgc gcttaaaata attatccaga ttaaaggcaa tgcgcggcag gtatcccaga
     4801 tcttccaaca aggtaaacaa agggaaaaaa atggccatgg gcggcagcat aaccgatacg
     4861 acccaggcca gggtccggta cattcccaaa accaacagcc cgtacagcca ctccggccag
     4921 ccggcagata aaaagatctg ggtcaagcgc tcctgtcccc agaagaatcc catcgccagc
     4981 cattgggagg gaaagttggc tcccttgacg gtaatccaga agatgatccc caacagcacc
     5041 agcataactg gaatcccccc cgcccgggag gtcagaatcc ggtcgattct gcgatctgta
     5101 ctgttataat cctcttttcc ataccgcgtt acattttgcg caatctcctc agcccgggcc
     5161 acaatgcaag taacaatatg atcgcgcagg ctgtgatcat cggtgtattc ttcccgcaac
     5221 atctgtccgg ttaaggccag aacagggaaa agcttttgac tgtcacccat ccttaaaaag
     5281 ggaaactgtg ctgccccttc ttccctttcc caagccctgt caagcaactg cagggcatgc
     5341 cagcggcatt ttatcttccc ctccaccatc ccctccatgg caggaataag cagggcaagc
     5401 cctttttcca ccgctgctcc atatttcacc cgcaaaggtc tggttggcaa tgtgccgctt
     5461 gccacctgcc agacagcatt tttcagagca tccagtccca ttccgttgcg ggcattggtc
     5521 ccgatcaccg gcacaccaag gatattcgcc agtgccggca agtccagctg aatttgtttg
     5581 cgctcggctt catccagcag attcacacac agcacaacct gctccgtcat ttccagtacc
     5641 tgcaacacca gatgcagatt tctggccaga caggtggcat cgacaactac caccgctacc
     5701 cttgcttctc ctgaacaaag aaaatcccgg gtgacttctt cgtcagccga gttggccgcc
     5761 agagagtacg ttcccggcaa atcgacgagc acgaatcttt gcatttgata aagatagctc
     5821 ccccgtgcat tggcaaccgt cttgcccggc cagttgccgg tatgctgttt gagcccggtc
     5881 aagcggttaa acaccgtact tttgcctgta ttaggattac cgaccagcgc tactacgaga
     5941 gcttcctctt gacagtctgt gcgatccgta tctcttggtt ggctgaacat ttgtctgtga
     6001 ttcacgttta tgatcctcag cttgtcggct ttaaaagact tccaccagga tttgggaggc
     6061 ttcatcagcg cgaagggcga tgaccgcacc gcgaatctca taggcaaccg ggtcaccgga
     6121 gggacttttt tgcaaagctt gcacccgcgt gccgcagatg aaacccaaat ccagcattct
     6181 ccttcgggta ttgccattcg ccgtcaattg acttactttc ccgcaagttc ccagtgccag
     6241 acgatgcaac ggaatcagtt tatccatctg caagcctcct aaaatattca tgctttgctt
     6301 cccgttatca taaattcgac cagggaaaat gatttgcaca tgtctcattt tatgaagcac
     6361 tgacagaatt ggttacatgc tggtgataaa gagcaataaa tcttccagga aaaaagcaac
     6421 ttgcatcaac aatgtttcac gtgaaacatc tatatcccga tccccagcca aagtccggcc
     6481 cgatagacca caaaggaaac cacccaggcc aaaacgatcg tataacccac ggtaaatagc
     6541 ggccatttcc agccgttggt ttctcgcttg atgaccgcga tggtcgccag acagggaaca
     6601 tagatcagcg tgaacaacat cagcacataa gcaaccagcg gggtaaaccc tgattgttcc
     6661 ctgactctct gggcaaagct ctgcaccgca tggtcctctt cttccgccag cacatcgctg
     6721 tcagtaatac tgtacaaggt tcccatcgtg cttactacaa tttccttggc acccaacccg
     6781 ctcaccagcg ccacaccggt tttccagtca aaacccagcg gttttatcag cggctcaatc
     6841 gccttgccga ctttgccggc ataactgctc tccaattgct gcgtggcatc agcgaattgg
     6901 ttttcctggg cattggtcag ggggaaggta aacaatcccc acataagaat ggacatcgcc
     6961 agaatcaggg taccggcttt ttgcaaataa agccaggttc tctcccacat gtgaatcaac
     7021 agatacttca gggttggcat ccggtaaatt ggcaactcca taacgaatgg ctcggtttca
     7081 cccgggagca gataagtacg aaaaattcga gccatgccaa tcgccagtat aataccgatc
     7141 aagtacaagg aaaacagcac gttgcccccc gcctgcgggc taaagaaagc agcaatcaat
     7201 aatgtatata ccggcaaacg agccccgcag ctcattaacg gggtaaccag catggtcacc
     7261 aaacgatcgc gcgggttctc cagggtgcgg gttgccataa tggcgggtac cgaacaaccg
     7321 aaaccaatca gcatcggcac aaatgatttt ccgtgcagtc cggcacggtg catgatgcca
     7381 tccatgacaa aggctgcccg ggacatataa ccggtgctct ccagtaaggc aattcccaaa
     7441 aacagcagta caatattagg aagaaatacc aacaccccac caaccccgcc gatgattccg
     7501 tccaccagca aagaactgag caggccttcc ggcatttggg ccaacaccca accggccagt
     7561 gcggataccc cgacatcgat ccagtccata aggggggctc ccagagcaaa ggtcaattgg
     7621 aaaagcagcc acatgatccc taaaaatatt ggtatcccaa gcgcacgatt caacaaaacg
     7681 ctgtcaatct tttcggtcac ggacaatcga tccatcgcat gggctttaca agcttcgtgg
     7741 tataccccgc gaacgaatgc gtagcggtct tccaccagac aggtttccgc atcatcgcca
     7801 atcagatttt gaattcgttc ccgagattta aggatatccg gcagcatctc gggatgaact
     7861 gtggcgatca gttgagtgat ttcccgatca ttttccagga gtttaaccgc cagccagcgg
     7921 ctcggatacg tcaaggcaac cgcagagccg ctttcggtac tggcggcgat ctccagcttt
     7981 ttttgacctg ctgcgcccat ctgctttcgt aaatagagca attcatcttc gatttcatga
     8041 cggtaacgga caactctttc cggaaactgg cgctcgtttt ccacgctcct taaggcttgc
     8101 tgcaaaatct cttctgttcc ttcgtttttg ctgcccactg ctctcacggt cggtatgccc
     8161 agcaaggttg ccagcaatgt ataatcaatt ttgttggact gttcagccac atcggccata
     8221 tttaaaacca gcagcaaagg aacttccaat tccttcaact gactggtgag atacaaattg
     8281 cgttccaggt tggaagcgtc tacaacattg acgatgagat ccggtttttc tttgagaatc
     8341 acattgcggc tgaccagttc atccggtgaa taaccggata atccataggt tcccggcaag
     8401 tcaattacgg ttatctcata accctggtat gtgattttgc cttctttttt ctctaccgtt
     8461 acccccggat aatttcctac atgctggcgg cttccggtga tattgttaaa aatggtggtc
     8521 ttgccagcat ttggatttcc ggccagcgca atcaccagtt ttttatattc cattttcttt
     8581 cctcctcgtc tgctattcca aaataatggt atcggcttct tctttccgca gactcagatg
     8641 aaaaccatga tttttcactt caatcggatc gcccagcggc gcataacgtt ccatcaccag
     8701 ctccgtgccg ggtaccaatc ccatgtccat gattcttcta cgaatgggac cgttgccttt
     8761 gaccatctta atcctgcctt tttctccggg ccgcaggtct tttaatgttc tcatccttct
     8821 ctctcctctc tgtcaatttg gattttggat ttacaattgt tattccggca gcttccgcaa
     8881 gacgagcaat ccagttggaa atcatcctgt accaagcccg ttattctttt ttttactctg
     8941 gtaatgatat acagcaaggc cagcgtgata atcgaaatta aaatcatttt ttccattgtt
     9001 gtcaactcct ttcttcagtt agtagtacct aacatttatt gaacttttaa accccttgcg
     9061 gggttttgat aaaaattatc aacttggcaa taaaagctta ttaaaatttg tttttactgt
     9121 caagtgtttt tagcattgct gccagtgttt ccgtactgat aacgtgttcc atcatgcagg
     9181 catcgcgttc cgccaccgca gcggaaagct ttaatacatt ctgcaaatat cttctgataa
     9241 tcaagtgacg atgccaaacc tgttctgctt tctgtcggcc tgtttcggtc agataaaccg
     9301 gtccgtaacg atcttgggta accaaaccgt cggctcgcaa atgatggatg gcttggttgg
     9361 cgctgggctt ggcaatccca agacgactgg caatttcggt gatggtagct ttgccgtttt
     9421 cttctgtcag gcgcaaaata atttccaaat aatcttccag agccggagtc agatggttat
     9481 ccatgccgcg tcacttcctt tcatataatg ttattataac ctaacttcag caagaataaa
     9541 agcacttgct gtccggtcag gcacacccat aaaaaaaggc tgtccagcag cggacagcct
     9601 ttttcaaaca gcctctgttc tgattctatt tatacatttc ctccacctgc gcggcgaagt
     9661 ttttcagaat cacgcgcggc tttagtttca gggtgggtgt cacttcctca agcggttcca
     9721 ggaaacggcg gctggagatg gtatattttt taatgctctc gtagttttcc agttccttgt
     9781 tggcctcggc gatgtcttca tcgatcagcg cccgcagctc cggactgccg gccagatcgt
     9841 cgccaacctg cacgatgact ttggcggcgc cttcgccgac ggaaacaatc ttggattcat
     9901 cgaaagctac tcccttggcc tttaacaagg gaataaccac ttcaaatttg ggcaccacga
     9961 tagcagctac atattttctt tcatccgcca cagcgcagat ttgttccaca taacgagcag
    10021 tggagaactt gccttccact ttggcgcggg gaacattttt gccggtatcg agcaccatga
    10081 tgctcttttt gcggtcaatg atcctgtaat aatccacacc cagcttgctg ttgtgcactg
    10141 ttattgcaat gtcaccggta tggaagaaac cgtcttcgtc aaatgcttcg gcggttgcct
    10201 ggggattgtt gtaataaccc tggaaaagca tgggtcctct gaccagcagt tccccatcct
    10261 cggcaatttt ttcttccgca tagtaattgg tcggtcccat ggaacctggc aacaccgctt
    10321 ttaagttgct gaagttgact gcattcatgg tctcagtcag accatagcct tcgcacactc
    10381 tgacgcccat cgccattaag gatttgcaca ggttgggggg aagcccggcg gaggcggaaa
    10441 aagccatttt atagttgggt cccaggatat atctggcttt gctgaatact gcctgatcag
    10501 cccactggta ttctttcttc agatcttccg gcagcagatc cgccatgttg acatccagcc
    10561 ccatgtcgat gaagccttcc tcatcgctga catagtcgac cgctttgatg ccgatttcca
    10621 tggctttttc aaaagcggcc ttgccttccg gggttgcaga agctgcatcc cgcagtgcca
    10681 tgtagattct ttcgaaaatt ctcggcacgg aacagaacca ggtgggtttg aacagttgca
    10741 gatcgccgac cactgtctgg ggggattcgg cgtaggcaat ggtttcaccc aagcagatgg
    10801 actgcatttg gccgcactgt ctttcgtagg tatgggacaa tggcaggaag gaaagcaaga
    10861 catcatattc atccagcatg caatcattcc gggtccacat attgttgtcg gcattgaccg
    10921 catacatgaa gcttgagtgg gtgtgaaccg cgcctttggg atcacctgtg gtgccggagg
    10981 tataaataat actggccata tcccagagct ttatttcatc gcaggctttt tcataacaat
    11041 agggattctt gtgcatgtaa atgcgtccca gttgctgcaa ttggctgaga tggatgaacg
    11101 ccgcatggtc aggcagcgaa caatcgtctt ccatcacgat gactttttcc agagaaggca
    11161 tgtcattcag aacggaataa actttatcca ccccggcctg gtccttgaca aagataaatc
    11221 tcgaccctga gttgtttacg atataggcca tttcacggac ggaaaaagac ggataaatcg
    11281 taaccgtcac tgctccgctg ctgaggatgg caaaatcgga ccaaagccat tgcgggcaat
    11341 tgtaggccat aatggctgcc cggtcctgtt tcttgatacc catggacagc agaccggcag
    11401 ttaaatcgcg gactttcatg cctacttcgg cgaaggtcag ggaaacggtt tcggtcgaac
    11461 ccggagccac tttaaaccac tggcagcggc gttcgccaaa tttcattgcg gcacggttca
    11521 atagttcggg taaagtacac atctgcagct gttctcggtt aatcatacat tcacgctcct
    11581 tattaagtat cttgatacgt acagtattat tatactgttt atgaatgata tgcgcgaatg
    11641 ttttaaattt tattacaacc agcaatacat tttttattta ggcaaacata ctgcaaaaac
    11701 agatccttcg cccggcttgc taatcacggt aattgatcca tccagctttt ccaatatgtg
    11761 tttggcgatc gccaatccca gaccgctgcc gccggttttg cgcgaacggg ccttgtccac
    11821 gcggtaaaac ctttcaaaga tgcgctgctg ttccggttcg ggaatgccca cgccgctgtc
    11881 aatcactttg attatgatat ggctttccga ctcttccagc tgcagtttga ccgtcccgcc
    11941 gggggcactg tatttcaccg cattatccag cagattgttg acaacctgca tgattaaatc
    12001 ggggtcactt tctatcattg gcttgccggc aggcgcaaga tattccaggt tgatcatttg
    12061 cgtccggctc tgatgctgca tgacctgcaa ggcctggcgc aagaccgcat caacatccgt
    12121 aggctgaaag gccaccacgg attgttccgc ttccagccgt gacagttcca gcaagctatt
    12181 aatcatggtc ttgaggcgct gggcttcctc atagatgatc cgactgaatt cctgcacggt
    12241 ttcctgctct tctccgcttg tatgcattag tgtctcggca aaaccactga tggaagccaa
    12301 aggcgttttg agttcatggg atacgttggc cacgaaatct ttgcgtatct ggtcaatccg
    12361 tttcaggtca ctgatgtcat ttaaaaccgc caggactccc tgatgactcc tttcatctcc
    12421 gccaatcgga acaatgttga cctcgactgt tttgccgccg gattgatgca ggacgagttc
    12481 tttgtgtaac ggctgccacc cggcttccac ttgactgatc atttccagca attcataatg
    12541 attgatgact tccgttttgc tgcggccgat cattacttcc gcaatctgca gcagcttcag
    12601 ggcagccggg ttggcatacg agacaatgcc gccgggatct atcagcacga tcccgttgac
    12661 cgtgttctcc agcaaggtct gaaagcgatt tttcatgtca ttcaaggcaa tcatgccttt
    12721 ttccaggttc cccgacatat cgttgaaagc gctggccaaa taggccattt cgtcatcacc
    12781 atagcagctg atccggcgtt ggaggttgcc gctggacaaa tcacgggtgg ctgcagtaat
    12841 ctcaaccaag ggccctgcta tccggctggc catatagagg ctcagaagaa gtgccagcgc
    12901 tgctgatatg aacaacgcca gcagcatgat catcaaaatc ttatggttaa tggcggctaa
    12961 atctgacaac ggcatggcca gtctgaccac accggtctgc tgctgatgtg caaaagggac
    13021 ggctacatac agcatgttta ctttttccgt ggcgctgtag cgtatggcaa cacctgtttg
    13081 accgtgcagg gcctgataga cttcgggtct cgatttatgt gtctgcagct gactggcatc
    13141 gtaatccgaa tcccccagta cttgtccgtc ctggtcgata atactgaccc gcatcgcact
    13201 gtcacgggct gccgtggcac acagttcatg atatgttgct gccgattcgg gctgcatata
    13261 tttgatgata tcagcaatca gcatggcctc ccgggtcaaa cgctgctgca aattatgcaa
    13321 ataataatct ttaaaaccaa aataggtcat cgcgccgccg attagcagca gcattaggat
    13381 aatcagcaga tagctgccgg tcaatttgcc gcgaaaactt ttaaacatta tcattcacct
    13441 caaatttata ccccaccccg cgaaccgttt ttatatagac ggggattttg gggtcgtttt
    13501 cgattttatc ccgcaatttg ctgatatgta catccagcac ccgggtgttg acgccgtccg
    13561 catagtccca taacaattgc agcagatact cccgtttcaa tacccgttcg tggtttctga
    13621 gcagaacttc cagcagttcg tattccttta atgtcagatc aagccgttgt ccctgatggt
    13681 atatctcata ttgatctgct ttaatgataa aatcgccgat gcgggtttcc ttggtttccg
    13741 gaaccggctt tgtcatcccg cggttgcggc gcaggattgc ctttacccgg gcattcagct
    13801 cccttacact gaagggcttg gtcatatagt catccgcccc cagttccaaa cccaggactt
    13861 tgtcgatttc ttcttcccgg gcagtgagca tcagaatcgg tgtatcgttg tcttcccggc
    13921 gcaaatcccg gcagatttcc aaaccgtcct tgccgggcag catcagatcc agcacgatta
    13981 gatcaggttt ttcctctctg atcatttgcc aggcaacatt gccgtcatcg gcagcgagaa
    14041 tggtaaaccc ttctttggca aggttataac caatcaatct gaccaatgac tcttcatcat
    14101 ccacgaccag aatttttcca tccatatcca cccgctcctt aaaacgattt aatcattgtt
    14161 aattgatata tgcgcgtttt gcataaaaac ttttttagta aaccatatga aatggatttg
    14221 agaaaccggc aagggtacag gctgtgtttt gtcaatgctc ttgtgtaaat cgataaataa
    14281 tgcaaaatgc tcatatgtta atcctatact gaaaagcaat aattataaac caccggttgg
    14341 actggatttg ttttttttta ttaaaagaaa ctgtctgcaa gtgatttatc ctcctttagg
    14401 tatacgatta cccggcaaaa caaaaaataa aactgatcat gccaagaaaa acaagctggc
    14461 aatcgctcag caaacttcgc ataaaggagc aaacagatgg aaaatacagc atgggaaggt
    14521 tttcaaaagg gtgcctggaa cgaggagatt gacgtacagg atttcatcca gaaaaactat
    14581 accccctata ccgacggtga cgatttcctc tctccaccca gtcagcggac cttgcagctg
    14641 tgggaaaaag ttaaagcttt aatgcaggag gagcattgcc gtcagggggt ttactcgatt
    14701 gataccaaaa cagtctcctc tatcacctct cacccgcccg gttaccttga tcgtgacctg
    14761 gaattgatcg tcggcctgca gactgatgaa cctttaaaac gatccatcat gcccaacggc
    14821 ggcatacgca tggtgcgttc ggcagttgat gcctatggct acaaactcga tccggaaatc
    14881 gaactgattt tcacccatta ccgcaaaacc cataatcagg gggtttttga cgcctatacc
    14941 caggaaatac gccaggctcg caaagccggc atcattaccg ggttgccgga tgcctatgga
    15001 cgcggccgga tcatcggtga ttaccgccgt atcgccctat atggaacgga tcggctgatg
    15061 gaggaccggc aaaaactgat gaccggcctg gagcgggaca attttaccga agaattaatc
    15121 cgccagcggg aagaactcac cgaacagctc gccgccttaa aagaactggc ggaaatggct
    15181 gccgcctacg ggttcgacat tacccgcccc gcccgcaacg cacgggaggc ctgccaattt
    15241 gtctattttg gctacctggg ctcaatcaaa gagcaaaatg gcgccgccat gagcctgggg
    15301 cgggtatcaa ccttcctgga catttatctg cagcgtgatc tgcagcgcgg tgacatcaac
    15361 gaagagttcg cccaggaact ggttgaccag ttttgtatga agctgcgcct ggttcgcttc
    15421 ctgcgcactt ccgagtataa tgaattattc tccggcgacc cggtttgggt cacggaagcc
    15481 atcggcggca tgggcgtgga tggacgcagc ttggttaccc gcagctcttt tcgtttcctg
    15541 cacacccttg cgaatctagg cccggcaccg gagcccaata tgacggtttt atggtcagcc
    15601 aacctgccgg ccccttttaa aattttttgc acccgcactt cgattgccag cagctccatc
    15661 cagtatgaaa atgatgacct gatgcgttct tactggggag atgattatgg catcgcctgc
    15721 tgcgtttccg ccatgcgcat cggcagacaa atgcagttct tcggcgcccg ggccaacctg
    15781 gccaaagccc tgctgtatgc catcaacgga gggatggatg aattgctggg cttgcaagtt
    15841 gcacccaaca tcaagccgat caccgccgac caccttgatt ttgatgaatt gtgggagcgc
    15901 tatgaacaag tgctggcctg gctggccaaa acttatgtca ataccatgaa cattattcat
    15961 tacatgcacg acaaatatgc ctatgaaagg ctggaatttg ccctgcatga ccttgccctg
    16021 caccgcacca tggcgtttgg catcgccgga ttgtcagtgg tcgccgattc cctggccgcg
    16081 gtccagtatg cccgggttaa agtgattcgc aaccggcagg gtttggcgat tgactatgaa
    16141 gtggaaggag aatacccagc ctttggcaat aatgatcccc gtgccgacga actggccgtc
    16201 aaggtacaga aaacctttct cgaggaactg aaaaaacatc acacctaccg caatgcacag
    16261 ccaaccttgt cgattctgac catcacttcc aacgtggtgt acggtaaaaa aaccggcagc
    16321 accccggacg gacgcaaagc cggtgaaccc ttcgcccccg gtgccaatcc catgcaccag
    16381 cgagataagc gcggtgccct ggctgccatg aattcggtag cccgactgcc ttatcggtat
    16441 gccctggacg gcatctccta tactttttct atcgttccag ccggcctggg gcgagaggag
    16501 acagaacgac ccggcaagct ttctgcctta ctggatggat atttcgccca aggcggccat
    16561 catatcaatg tcaacgtcct ggaacgggcg accctggaag atgccatgca gcatccggaa
    16621 aaatatcccc agctgaccat ccgggtgtcg ggctatgcag tcaatttcat taaactgagc
    16681 aaagagcagc agctggacgt gatcaaccgc acgatccatg cggcattgta accactccgc
    16741 gaattggcgg catgcgcgat tctccatgaa gagcgccagg ggtacatgga gttcttgcgt
    16801 ttaacgtttt gacgagttta caccgatttt agccagcgac cacgacgttt atctggaggg
    16861 taagacatgc tacatgaagc ctatcttcat tccatcgaaa ccatgggtat gctggacggg
    16921 cccggcattc gctatgtgct gtttatgcag ggctgtccgc tgcgctgtgc cttttgccac
    16981 aatcctgata cctggcacat gagtaaaggt caggttaaaa gcctcgcctg gttgctgacc
    17041 gatatcaaag actatcttcc ctattttcgc gcatccggag ggggggttac ggtttccggc
    17101 ggagaaccgc tgctgcagac ggattttctg gaagttctgt ttgcggaatt aaagaagctg
    17161 gatgtccatc gggcaatcga caccagcggt tttgcagcgc tggagaaggt aaaattttta
    17221 atgggggtaa ccgatttggt gatgctgtcc atcaaacatc ccgatcccga taaacaccgt
    17281 gagatctgcg gacaatcgcc gcagcgcccc ctcgccttgg cccgttatct gaccagtatc
    17341 aagctgccag tctggatcag atatgtaatc atccccggga tcagcaatct cccggcggat
    17401 ctggcccggc tggctgacct gattaaatcg atgcccaatg tagaacggct agaattgctg
    17461 ccctatcaca ctctgggcag gcccaagtgg gaacagttgg gcctaagtac accgctgttg
    17521 acagttcctc cccccctgcc cgaggagatg gaagctgccc gccaaaccat ggccgggctg
    17581 ctgcccggcc tgaaaatcgt ataaacttgc gctgacttgg gatttttatc gctacccctc
    17641 cacagaggtt tatgtttttg tgcagcccaa ggggcaaggc aacagccgtg tgcttaaaca
    17701 taacgacgca aagagcgctt accgtttaat aaatacattc gtacccaaaa acgagactta
    17761 cgtatttgtc tcaagacccc gaggtaacaa atgcgcgctg atactgtaac tatagccagt
    17821 caaaagaccg gccccaagcg gaacgaatac ataaacatca gacaaaaacc acagtaaata
    17881 cgcaagtcaa gaaccgtccc caacttgcgt ggattagacg tcaaaccagc tgccgcctac
    17941 gaattcgcgc aatatcgagt tgggcgggat cggatccggg acgatggagc ggaaatagct
    18001 caggaatttc aacaaacgcc gcgcttcagt atattcgcgg tgatccgcac tgatttcctt
    18061 taacagtggt tcggccagcg gcttcagggc ggccagttca tagaccaggg agctgttgaa
    18121 catgatgtcc gcattctcct ggaaaggaaa aatgtgtttc tcctctcccc ttctcaccga
    18181 aggccagcgt ttgatggtat ttaaggccga ataaccacga gtccggctgt cgcggatgat
    18241 gcgcctcagc aagcggcaat cggtagtagg aatgcggttg gcataatcaa tgttgagttg
    18301 ggtaagggca ctgacataga ttttaaactt ttgttcgcgg gggatgctcc aggtcagctg
    18361 gtcattcaag ccgtgaattc cttcaataat aatcggttct cccgccggga cgctcagcat
    18421 tatgcccttc tccaggcagc agccttgctt gaagtcatag atgggcatct cgatctcttc
    18481 tccttcaacc aggcgggtga gatgctcgtt gaacaaatcc agcttcaatg cttcgatggc
    18541 ttcaaaatca tattccccgt tttcatccag cggggtcagt ttttgatcga taaaataatt
    18601 gtccagggaa atggataccg gccggcaccc attgacccgc aactggatca gcagacgctg
    18661 agcaaaggtg gttttcccgg aggaagacgg accggcaatg agcactactc gtaccccggg
    18721 attgtgtaca atcatatcag caatctgggc aatctttttt tcctgcaggg cttcgttgac
    18781 cctgattaaa tcatcgattt tgccgttttc aataatatca ttcaaggcgc ccagatggga
    18841 ggtattgagc atcccggccc aggctttggc ttcctggtaa atctggaaca gtttgccttg
    18901 ttcatgaaaa ggctgactga gatcaggatc attgggttcc ggggtctgca aaaccagtcc
    18961 cggcggatag tagattagtt tgaatttgtt taagactccg gtatggctga ccatgtaacc
    19021 gtaaaaatat tcataaaagc cgtccaattc ataaatatgg acttcttctt tatcgcggtg
    19081 ggcaaggatt ttcaccgtat ctggtttgcc ctgccgggcc agcagttcct gggcctgctg
    19141 tttgggcaga cgttttcttt gaatgggcaa atccgcctcc acgatttctt gcatacgcct
    19201 ggcaatctgc tcaacctcaa cattgttaat ggtttcatcc agaaactcac agtaggtgcc
    19261 gttggacagg gtgtgtttga tcatcagacg gcgttcaggg aacaggtcct tgcacgcttt
    19321 gagcaacagg aacgaagcac tgcggcgata aattttcaga ccggcttccg aatataattc
    19381 gaccatttcc acttcgcagt cgcgatccag caagaagttt aaatcccgca ggcggttatt
    19441 gacaatcccg gctacgaccg gcaactcatg agacagctgc aatccggcaa tgagcgcttc
    19501 cagagagatc cctgcagccg cagtaatgtt gcccagtcct ttgaccagca cattgatctg
    19561 gtttgttttc gctgacagtt tggacaatcc tatctcctcc cttcagaaca ttatacacga
    19621 ttgaccaaaa gggggacact actgcccggc agaatatccc ccttttcata atagatatgc
    19681 ttttgtaaag agatttatca gcgcagacgc acgtcgagtt tgtctttgat tgcatggaca
    19741 atggcctcca tgacctgggt gacttcagct tctgtcaaag tgcgttctcc agattggaac
    19801 accatgcgat aggccagact tttatgtcct gcttccagcc tgtcaccggc atagacatca
    19861 aacaagctga ccttctgcag aagaggcccg ccgctgtttt taatcacctc gacaatttga
    19921 tcggactgta tctcttcccg cagcagcagg gcaatatctc tttccacagc cgggtagcgg
    19981 gtgatgcttt ccatcatatg tttgggactg gacaaatcaa acaagcggtc aacatccaat
    20041 tcgaaagcac aaacccgcgg tttgaggtca aagttctgtc tcaccagcgg atggacttcc
    20101 ccaatgacgc ccagttcctg tccttgacaa agaatccgtg cacagcgacc gggatgataa
    20161 gcgtccaaga cgacatcctt aaattgagtg ttttcaatac ccagctggga caggaagttt
    20221 tccaaaatac ctttcagata gaaaaaatcc atttctacat tttgtttaag ccaattgctc
    20281 tccaaccgtc cggccactac tgccccgagt tttaattcct cgcggggcag ttgttcacct
    20341 ttctcgggga aatacacctt gccgagttca aaaaaggcca aattgctgtt tttccgggct
    20401 agattgcggc tgacggtttc caacagcccc ggcaggagca tagtacgcat cacgctttgc
    20461 tcttccgaca agggattggc cacctgcacc gtgtgacgca gccgattgtc ttccgccaag
    20521 ccgagcaggt caaaatagcg cgggctgata aagctgtaat taatgacttc ataaaaagtt
    20581 ctggccatca ggttctggat tttttcccgg aaatgctgat aggggctcag tcctccccgg
    20641 gaagccgcac cgggaaacgt agccggaata tgttcataac cgtgcagccg ggccacttct
    20701 tcaattaaat cggcttccag ctcaatgtcg ggacgatagc tgggcacctc caccatcaac
    20761 tgaccgtctt tttcttccag cgaaaaccct agatcatgga tatagccttt gatttcctcc
    20821 gtggtcaggt tgatgcccag caaagcgttg atccgccggg gacgcagtcc gaccgtccgc
    20881 agggtctggg gagccggata gcagtcgcaa atgccctgta ccacttcccc ctccgccagt
    20941 tgctgaataa ggtccgccgc ccggttcaag gcataaatca gtccattgac gtccgcacct
    21001 ttttcaaagc gcatcgatga atcactgcgc agcgccagtt tgcgtgatgt tttgcggatg
    21061 ccgacgccat aaaaacaagc tgattccaac agaacattga ccgtatcatc gctgatttcc
    21121 gtattctgtc cacccatgat gccggccagc gccaccggtc tgccgtcatc ggtaatgagc
    21181 agcatctcct tgtccagcat tctttccaca tcatccagcg tcacgatctt ttcctctgct
    21241 tcggcccggc gcaccaggat tctcttggcc tggctgagca gatcgtagtc aaaggcatgc
    21301 aagggctgat tggtttccag cataacgaaa ttggtgatgt cgaccacatt attgatggga
    21361 cggatgccgc tgttgaccag acaggtctgc agccactccg gagatggttt gatttttaca
    21421 tttttgatca cccgggctgc ataacgcaga cacaaatccg catcggcgat gtccactttg
    21481 atatagtcat ctatcttttc cccgttttcc ctgattttta acggcgggat cttgatttcc
    21541 tgatgctgca gcgccgccac ttcccgggcc agatggatca tgcccagaca atcaccgcga
    21601 ttgggggtca aatccagttc cagaacactg tcgccgttta tctcatccac gccttcaatc
    21661 gcaatcccgg ccatggtcag ccgatgcgcc aattcctggg gggaccaggg aatgtctaca
    21721 tattgtttca gccaattgat tgatactgcc atcatgccac ctccttaaaa ctgcgtaaga
    21781 aatcttttat cattgtcaaa gaacaggcgc aggtcattga tgccgtgttt cagcatggcg
    21841 acccgctcga tacccatccc gaacgcaaat cctgatactt tttccgggtc gtacccgccg
    21901 ttgagcaata cccgcggatg taccatgccg gcacccagaa tttccagcca gccggtatgt
    21961 gagcaaaccc ggcagccttt tcctttgcag atgacacagg agatatccat ttccgcactc
    22021 ggttcagtga agggaaaaaa gctgggtcga taacggaccc tgacgtcctc cccgaagact
    22081 tgtttggcaa aggccatcag ggttcccttc aagtcgccga aggtaatccc ctggtcgacc
    22141 accagccctt cgatctggtt gaacatgggc gaatgggtgg cgtcgtcatc tttacgatat
    22201 accttgccgg gcacgacgat tttcaccggc agctgcggcg ccattttttc catggtcctg
    22261 acctggcacg gagacgtatg ggtgcgcagc agaatatcct cggtgatata aaatgtatcc
    22321 tgcatgtcgc gggccggatg atccttgggc acgttcaagg cagcaaagtt gtaataatcg
    22381 ctttcaatct ccggcccttc cgctactgta aagcccatcc cgataaagat catctggatc
    22441 tcgcggatca cctgactgat gggatgaagt cctcccgtct gcagcggcag tccgggcaga
    22501 cttacatcga ttttttccgc cagcagggtg cgttccaatt cgcgggctcg aatgtcgctg
    22561 gtcctggcct cgatcagatt ctcaagaata gccttgattt cgttggccgt cttgccggct
    22621 tccaccctgg catccccgct caggtctttc agcccgcgca gcagattggt caattcgcct
    22681 ttccggccca gtacttttac cttgatttca ttcagggctt cgatatcact gacttttttc
    22741 acggcttcct cggttgcttg cctgagctgc aaaagtctgt cgatcatgtg acccatcctc
    22801 ccatgtaatt gatatgtaat aaaaaaagac cttcgtcgct agggacgaaa gccttgattc
    22861 cgcggtacca ccctgattaa ccgatgcaga ggcatcggct cacttcgggc cattaacgtt
    22921 ggccaacggg cagacctact aatggttcag tctgccgctc ccaggtgaat tcacctgctt
    22981 tcccctggct gtactcgcag tctttgatac agcctccctg aaggttttca gcagattact
    23041 ggtcctgttc atcacgttcg caattattgt gttgccatca ttttatacag aagatagtaa
    23101 ataatacaac cggaagtttc aaaaaggctc cacaaacttt cagtttgttc tctcataacc
    23161 gttccaccct ccgttgcttg cgacaggtaa tgatttcctg ctattcactc tctccagaaa
    23221 gatctcaaaa aggactaggt tcttctttgc cgccaagctt ctgctgttat tatagcacag
    23281 gccgcagcca cattcaaaga atcaacgctg ggattgattg ggattctggc agtctggctg
    23341 cagcgacgtt gcaacgctgg atcaatcccg ctggcttcac tgcccaggac cagaattttc
    23401 ttgccccgca gatcgatgtc ataataatcc attgcatttt ccaaaccggt ggcgatcaat
    23461 tcgaagccat gcgcctgcag atcatcaagc tgccggtcag agaccgcttc gatcagggga
    23521 agatggaaga tggcgcccat ggtggaccga accactttgg gattgaaagg atcgacgcaa
    23581 tccggggtca aaagcagccc atctacctcc agcgcccagc agctgcgcat gatgctgccc
    23641 aggttgccgg gatcggcaag gtgattcaac aacaccaaaa agctgtctgc cgcagtcagc
    23701 gcggtcagat tccaatcagg cttgcgcaga attgccgcca tgccttgcgg ggtttcggtc
    23761 atactgatat gcttgagcag tttgtcgtca atgacatagc atgcaaagtc ctgccggtca
    23821 gcaaactcac cagccaattc ctctctgata aaaagcgcta caatccgatc cggcaaagcc
    23881 agcgcctctt ccaccaaccg ccggccttca gccagaaata agttttcctg atcccggtac
    23941 tttttcaggt gcaggctgcg ggccagtttt atcacctggt tttcggaaga actgatgaat
    24001 ttcattctct tcacctctcc tgcctgcaaa caggcagcgt gattgacact ctgtttgcgg
    24061 taaacaaaaa agcatggctt gcaggccata cttttttata cattaatatg ggggttgcct
    24121 tatacacttt tcttggcaag ttcagccaga tcggcgaatc cctggggatc actgatcgcc
    24181 agatcagcca ggactttacg gttgatgtca acgccggcta ctttcaggcc atgtaccata
    24241 cggctgtaag ttgtaccatt gtcacgtgct gcggcattga tacgggcgat ccataactgc
    24301 cgaaaatctc ttttcttgag tttgcgatgg gcataggcat aattgccgga cttcatgacc
    24361 tgttggttgg ctacccggta cagcttggac ttgctgccca cataaccctt ggccagtttc
    24421 aatatttttt tatgtcttct gtgggtggtg acaccccttt ttactcttgc catgacttag
    24481 ctcctcctcc gtctatttat aaggaatcag tttggaaatt ctcttttctt cgaccgtgct
    24541 taccagacca gctttacgca gattcctctt tctcttgggg gattttgaac caagtaaatg
    24601 actggcatat gctttggatc tctttatttt cccgctgccg gttttcttga accgtttcgc
    24661 agcagctcga tgggacttca ttttgggcat gatgaatttc ctcctttaat cgttgttctc
    24721 tattttaggc aacagaatca ttaccatgtt cttgccttcc actttgggca ttttttcaat
    24781 ggtcgaaatg tcagcaagat ctgcggcaaa tttctggcac agtttcattc ccagttcagg
    24841 atgtgtgatc tctcttcctc ggaacataat ggtaaacttg actttatccc cggaggtcaa
    24901 aaacttgcgg gcatttttcg tcttgacatc aaaatcatga tcttcgatgt tgggacgcat
    24961 tttgacttct ttgatggtaa tgactttttg ctttttgcgg gcatccttgt cacgtttgct
    25021 ctgttcaaac ttgaatttgc catagtccat gattctgcat accggcggtt tggctgaagg
    25081 ggccacctcc accagatctg cgcccttgtc caaggcaatc tgcagcgctt cacgaatcgg
    25141 cacaataccc aactgttcac catcttcact gaccagtctg acttccttta cccgaatatc
    25201 ctcgttgact ctccattcct tactgataat gaattcacct ccaaataaac ttaaaaaaag
    25261 agcgggttac aaaaacccgc tcctgaatgc catagccaag caattcccat gcactggtac
    25321 acgaatcatc attgcttgtt tacggtaacc ctgcgaacaa ccggttgcag ggtgagaagc
    25381 aaggcttctg cttaaaaaaa catctttatg agtatagcat atctatctgg caaaatgcaa
    25441 ccatttaaag cactatctcc ttatgattat agccttcgtc aatatacaaa gtaaatgcac
    25501 ccgcccgaag aaatagtcgt gttggttttc cgccctgcta tcgtcttttg caaacgatgg
    25561 cattttacaa ttcattgtat tttacactac actgcccaat cctaaaagga atccttgttg
    25621 gttttaaccc agattttcat attctgtgca tctttgatca attcatcacg gaacatggga
    25681 tgggcgatat tgatcagcgc ttccgcccgc tgccaggtgg tcttggcttt gagcatggca
    25741 atgccgtatt cggttactac ataatagttg atggtacgcg gatcggtgat gatgccgccc
    25801 ggcgtgatgg cgggcttgat acgcgatacg agctgaccat ccttgccttt gacggtggaa
    25861 ctcagacaaa taaatccttt gccgccggct gaccggtagg agccaaagat aaagtccaat
    25921 tgcccgccgg ttccggaaat ctgccggggt ccggaagatt cggcacacgc ctggccgaac
    25981 agatcgattt ctaccgcgtt gttgatggct acgaccttgt tgttttggcc gataataaca
    26041 ggatcattgg tataatcaac cggatacttg gcgcatatcg ggttttcatc caggaattca
    26101 tacagtttgt tggttcccat ggcaaaagtg tagacgatct tgttggcgta aacctgcttt
    26161 aatcggccgg tgatgcggcc ggcttcatac atatcaacaa aggaatcgac catcatttcg
    26221 gtgtgcacac ccagatcttt caagtcggat tgcgcaatca acgagccgat ggcgttgggc
    26281 atcgcgccta tgcccaactg cagacaagcc ccgtcctcga tttcattgag caccagttgg
    26341 gccacctggt tgtcgatgtc gctgatgggc gtttccggaa gctgaatcaa gggtttgttg
    26401 tccccttcca cgataatatc tatttctgaa atatgcaggg cttcccgcac tccgcccaaa
    26461 cactggggaa cagaactgtt gacttcgaca atgacggttt tggctgcatc acaaagatac
    26521 ggagtgatgg agctgctggt acccaggttg aaaaaaccgt gtttgtccat aggggcaact
    26581 tttaccatgg ctacatcaat cggtacatag ctttcctgtt tgttgtcggg caatttttct
    26641 gtgattgacg gaaaatttct tgaagaaaga aatttatcat gattcatgtc agccagattg
    26701 attgcccttt ccaaaatgtt ttccaactcc cggacattgc cgggccagga atagttttgc
    26761 agcaactgca ctgcccgggc agaaatagta aaaaggttgg tgtgcagttg tttattgatc
    26821 cggtatatca attcattgat gataatcggg atatctgcgg ttctttcgcg taatggcgga
    26881 attcttagcc tgaccacatt taaacgatag taaagatcct gacgaaattc cttgttaaaa
    26941 accatgctct ccaattcccg attggtagca gcgatcaccc ttacattggt ggtaatcagg
    27001 cgggtacccc caacccgctc caccactcgt tcctgcagaa ccgacaacag tttggtctgc
    27061 atcgttaagg gcatgtcccc gatttcatcg aggaaaatcg ttcccccgtt ggccaactca
    27121 aactttcctg gtttcccgcc ttttctggcg ccggtgaagg ccccctcctc atagccaaac
    27181 agttccgctt ccagcagact ctccggcagg gcagcacagt taatacgaac aaaaggtgcc
    27241 aggtagcgat tgctggcatt atgaatggca tgagcaaaca actctttgcc ggttccgctt
    27301 tcaccggtga tcaaaatcgt ggaacttgtt ttagaaagat gataggccag ggatttgatt
    27361 ttcataaatt cattatcact gccgatcaaa tcatcaaaat accatcgtgc ctggtagctc
    27421 tgacgaactt cttctttata aatattcaat tctttttgcg tttcctgcaa ttttttaatg
    27481 agcaactgcg ctccggacat atccaaaaaa atacttttgg ccatggcacc gatgatttgt
    27541 ccgtctttga cgatgggaag acgcgtgatg acggtatttc tgccatttat cggccagttc
    27601 cgagctcggc agaacatcca gaatatactg gcccatgacc tcttccggac ttgtttccag
    27661 cagttttagg taagtttgat tcatatcggt gataaaaccg ttgcgatcga cgacaactac
    27721 ccccatgtac ggactggcaa taagaggctc cagcatttcc atgaattcag caatcgtaaa
    27781 atttgccaag caatcgcccc cccataaatc ttgatagctt gtttataata gaattattag
    27841 gcgacgacgc tgttcatgcg catctttcaa tatattcaag acaacattcc gttttctctc
    27901 ttttcgatgc ccttgctcgg gcaatacatt gcataccaat aataaattct actctatcca
    27961 tcaaaaaaga gcgggttgaa aaacccgctc ccgaagcgtc taaaaggcaa tatgtttaaa
    28021 tgttgtgccg gttggcgatt tcatctttga tcgtggcgat aaaatcgtcc agcttctgac
    28081 tgcccagatc gccttgtttg cgatgacgca cagcaactcc gtccatttcg atttccttgt
    28141 cacccagcac cagcagataa ggaatcttct gcagctggcc ttcacggatc ttgtagccga
    28201 ttttttcgct gcggtcatcg atctccgccc gaatcccgcc ggctttcaaa gcggttaaaa
    28261 cattgcgggc atattcatgc tggcggtcgg ttatcggtaa tactctgacc tggacgggtg
    28321 ccagccaggt gggaaaggcg ccggcataat gctcggtcag aatcccaata aacctttcca
    28381 tgctgccgta gataacccga tggatcatga ccggacggtg tttttctcca tcttctccga
    28441 cataggtcag atcaaatctc tccggcatct gaaaatccaa ttggatggtg ccgcactgcc
    28501 aggtacgatc cagggaatcc tgcagatgga agtcaatctt cggtccgtag aaggcgccat
    28561 ccccttcatt gaccttgtag gcgatgtttc tttcatccag cgcttgccgg agagcatcgg
    28621 tggccaaatc ccaggcttcc tggctgccaa ttgaattttc cggccgggtg gacagctcaa
    28681 catgataggc aaagccaaac tgactgtaga acaaatccac cagattcata acgcctttga
    28741 tttccgcaat aatttgttcg ggcagcatga agatatgagc gtcatcctgg gtaaaagcac
    28801 gcactctcat caagccgtgc aaaacgcctg acatctcgtg ccggtggacc agtcccatct
    28861 ctccgatgcg caggggcaga tcgcggtaac tgtgcagatt ttgtttgtaa accaggatgc
    28921 ctcccgggca attcatcggt ttcagcgcaa aatcggtctc atcgatttcg gtaaaataca
    28981 tgttgtcctt gtacttgtcc caatgtccgg agcgttccca cagttcccgg tttaacataa
    29041 tcggggtgcg gatctcctga tatccggcaa tttcatgttg ttcccgccag aatttctcca
    29101 attcgtttcg caaaaccatg cctttgggca ggaagaacgg aaagcccggt ccttcatcca
    29161 gcagcacaaa caagcccagt tccttgccca gcttgcggtg gtcacgtttt ttggcttcct
    29221 ccaatttgtg cagataatca tccaactgcg cctgtttggg aaaggaagta gcgtagatgc
    29281 gctgcagcat tttgttgttt tcattgccgc gccagtaagc tcccgccagg ctcatcagct
    29341 tgatggcttt gagccgcccg gtggaaggca catgcggtcc ggcacataaa tccacaaagt
    29401 caccctgttc atagaaactg atgatggcat cttcgggcag atcttcgatc agctcgactt
    29461 tatagatttc cccaatggaa cggaaatacg caatcgcttc attgcgcggc aattcctttc
    29521 tcacgatggg ataatcgctc ttgatgatat ctgccatttc tttttcgatt ttggcaaagt
    29581 cttccggggt aaatgtatgt tcggtatcaa aatcataata aaatcctttg tcaatagccg
    29641 gaccgatggc cagacgagta tccggccaaa gccttttgat ggcctgggcc atgacgtggg
    29701 aagaagaatg acgatatacg tcacgcgctt tttcatcttt aaaatcaagc agtcgcagcg
    29761 ctccgtcctc gttgacaggg cgggtaagat catatacctg gccgttaaat tccgcagcga
    29821 tcacattttt agccagtttt gcacttattt tctccgccat ttccaggaag gttgacccct
    29881 ccgaaaactc gcgccggctt ccgtcttgca acgtaattgt aagcatcttt ataacctcct
    29941 gttaaaactt atcctgccaa taaaatgata acctgaacca gaccgatcat aaaccccatt
    30001 acgccaccca aaaccttaat aaaggtgagc tccggggcgg aaatattttt aatcatctct
    30061 tccagttgct gcaggtcaaa ggcattgacc ttttcctcca caattttgct gatatcgatc
    30121 tcatgggtca gaaattcgct gcccgattta aataactgtt caacaatctg cgcggattcc
    30181 tgactcaaga tcttgtccaa ggtatcgatg atgagttgga ttaatcgacc gggaataatg
    30241 cgcggcagca attcattcaa tcgttctcgc accaaggccg atattttggt cacgatcgtc
    30301 tgctggttgg cgggcgtgtt gatttgcacc agcagatcat caatggacag cagttctttt
    30361 tccaccagtt cgcccagact gacagcgatc tgggcctgcc ttttgggcag aatcccctgc
    30421 accttgtaaa agcccagatt gattggctgg cgaggccaaa acagcatttt aaccgccact
    30481 acattggtga cataaccaat aaaagtgcta atcaaaagtg ttgtagcgat cttgataacc
    30541 atatgatcac cctatcccat attccttcaa agaatagcac tttttcgagt ccatatcaat
    30601 aaattatgcg gcgagccctt ttgccctcgc caactgggtc agcatcttcc aagatcttaa
    30661 aagccgttca ggatgtagcc ctgccggcca gccgttctcc attggtccac ctcatagaaa
    30721 agcactccat tgccggttta tggcctgttg ccaaggtgtc aatcaaatta gcagaaaacg
    30781 tttgaaagat aaagatgaat aaaaaaaacg ccgccctggc agcactatgc tgaccggcgg
    30841 catgaacatg ccccattggg ctgtctcaat gcgcaagggt tagttgcttt tattgcgctc
    30901 tccttcctgg tgctggcgac acctttcgca gccgccgcag tccttgatcc gctctccgaa
    30961 gacctggcga atcaccttga ccggttcgga attgtcactg tttgaattat ggaggatgat
    31021 gcggcgcggg gaaatggtga tcaagatgct gaccagaata tcatccagac taatatcgtt
    31081 gtacaacaac tcgttggagt aataatttat gatcttttca tcaatgtcgg ccccgttgcc
    31141 gtcccacagg aaaaacaacc cgtcctggtc catccgcaaa ttgacctccg gcatccgcgg
    31201 ggtctgggtg tcgacaaaat aacgcagcag cctgacgaag tcattatact ctttttcatt
    31261 gttcagttcc tcacaagcca aatctactgc aaagcgcagt tcgttccaat aatcctgcat
    31321 gccgaaattg attaatcctt caatcaccag tttgtcgtaa tgataaatgt gatccatgat
    31381 tttatgggaa attttattct tgcgtccata gttaaacaat aggtttatgc tgtcattagt
    31441 attgcatcgc tgcaggaaat ccatcgcctt gtgccaaacg attttcagtt cctcgccatt
    31501 gagatgccgg gtccgtttgc tgatctcccg gcggaccatc ttgttctccc agtcgtgcag
    31561 gatgtgttct gccagaaatt ctgaaatttg atgcttgaaa atatagacga tgtcctcatc
    31621 cttcataaaa agatcgttat tgtcgccttc aatgaccaaa tccagcagca gcgtattgga
    31681 gtttgggcag acttccctgt ttatctgcaa aacaaagccg cgctcacgca gccagccaaa
    31741 tcgatgcgaa aaatcatccc acaacaggtt ttcctcggct aaagttgcga tctgcatttt
    31801 ataaatcatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcccacaac
    31921 aggttttcct cggctaaagt tgcgatctgc attttataaa tcatggcagg cctcctcaac
    31981 cattgcttac gttattatat gtgaggccga ttgccattat tcaaaccaat ccatggttca
    32041 atttttatta aaaaagattc aatccaaaaa ctatcaccaa tgaggctgtt aatttgcaat
    32101 aatcggcaat actctgtctc cttgcgagca atttcatggc catgaccaac tggatgcttt
    32161 ttccacaaaa aataccgggg ccataaaccc cggtattact gcttttttat ggtgggctgt
    32221 ataggattcg aacctatgac ttcctccgcg tcagggaggc actctcccgc tgagttaaca
    32281 gcccatgtca acaatataaa ttatataatc atcaaactgc aaaagcaagt caaactcgct
    32341 gtatttatac aacgatcttt tttttccgca tgtcgaaacc caacgttttg ttgagaatac
    32401 aaacatactc tgcctccttg cataataaaa tgtaccccgg aggaggtgaa atcttgcgtc
    32461 agaagaaaat aatcggctac ggatttatgt tcagcggcct cattctggtc agtttgctgg
    32521 taggatttta taaatttgcc ctgcattcca acatggaata ctggaagaag aatcatcttt
    32581 tgattgatcg ggtcaaaatc gccgaaaaag tggtggccct gacctttgat gacgggcctg
    32641 acccggcaac aaccccggcg gtcctgacca gcctggccaa acatgatgta cctgccacct
    32701 ttttcgtcgt tgggcagcac gctgaagcgc atcctgcctt gctgcaaaaa atctctgcgg
    32761 ccggccatga actgggcaac cacagttaca gtcaccggga ttttaaccac gccagcgggg
    32821 ctgagatccg ggatgaaatc cggcgcacca atcaaatcat tcatcaaatc tccggacagc
    32881 aaccggtctt gttccgtcct ccgggaggtt atttatccaa tgaaatgatt cgtatcaccc
    32941 aggaagaaaa agtcatcgtt gcctattgga gctgggaaac ggacagcaaa gactggcgca
    33001 gcagcaacag ccaaagcatt gccgatcata taaccggcca tattgcgccc ggtcagatca
    33061 tcatcttgca tgatggcgga aaaaacggtc tgaccactgc ccgggcagtg gatctgatga
    33121 tcaaagtgct ccagcagaag ggctaccgtt tcgtcaccat gggcgagttg atcaaaatgg
    33181 gaaatcagga ataaaggcga aattggccaa ataaaatata gaaaagcaaa aggaggatga
    33241 tacttatgcc taaagtcatc ggtttttggc atctgaaaaa cagcaccaag ctgattatca
    33301 tttccattct ggcaacgttg atcatgacca ccagcgtatt atatgtgcag gcctaccata
    33361 aactgaaacc aatttatcag attgacactg agcaaaaagt ggtagcgctg acttttgaca
    33421 tcagttgggg cgacaagacc ccgctgccgg taattgagat cctcaaagcc aaccaggtaa
    33481 aatcgacctt ctttttatcc ggtccctggg tgaaacaata cccggaaata ccaaaacgca
    33541 ttcaaaaaga cggtcatgag attgccagtc acggctaccg gcatattaat ttaagcaacc
    33601 tgagcaagaa tgaaatcaag gaagaaatta tgcaagccca tcaaaacatc agggaggtta
    33661 ccggcatcaa cgccaatctc attcggaccc ccaatggtga ttacaatgac caggtgatcg
    33721 aagcagcgca tgaatgtaaa tatgaagcaa ttcagtggag tgtggattcg ctggattgga
    33781 tgaatccggg cgtcaatacc atcaccgaga gagtcggcaa gaaagttcat cccggcgcca
    33841 ttattctgat gcatgccagc gacacctgca agcaaactac agaggctctg ccccaggtca
    33901 taaaaaacct gaaaagtcag gggtatgagt tggtcacggt ttcggaactg atgaaactgc
    33961 aaaagcagga gcggcgagac aagcagaatt aaccgatcaa aaacaatagt accgtattca
    34021 aactttatat gactttgcag aaatgcgaaa agccatttaa gtgtttccac ttaaatggct
    34081 ttaggcttga gaatcactgt catcttcatc aatatgttcg agtccgatgt ccctcaagat
    34141 ctcatcaatc aatgccttat ccaatctttc atctcccttc tgaatataat aaaaaatctt
    34201 ccgccatgat aatgattata aaacggcaaa tcaagttttg caatactttt ttgaattttt
    34261 atacatagtt tcctgcccca atcaaatact aagcacagaa aacgctgtgc ctggcagcag
    34321 aaaagaggtt tctatgctta gcagtgacgg agcctgggca cgttttattt tttctatttg
    34381 cttttttctt ctctcgggaa gcgaaatgat caccggttgg ctggcaacct tatccggggt
    34441 ggcaggaacc atcgaattgg ccacggcgtt gacacggtac tctcccttgc agaatttgtt
    34501 gcaggtgctc accgccagta cgccgggcca gggggcgcga cgggatcatt gattggtttg
    34561 ggtccacttt tctccatcaa attgataact ctcatatttt ccatcgggcg gacggaaacc
    34621 ttcccaattc gctttaggtt gataaaccag tttgaccgac agatcttttt gcacttccgt
    34681 ggcaataata tagcgctggg tatactgggt atggccgtct tccattgtca cataaaactc
    34741 gtcgccggct tcaccaagaa aatcagccgg aaagaaaacg tcagtggtaa aaaacgtgcc
    34801 ccccaacgtc gctttcggaa tatcattatc ggccaggtcg gcaaaatcgg cgaaaatttc
    34861 ctgttggctg atggctggtg tttctctgac cgtttccgtt tgattgtgag catagagaaa
    34921 atacgccgcc aaggccaggg caattatata gaatactctg cgaaaattca tattatatcc
    34981 ctcttttact tatttccttt tatatagatg attgcccaga tcttcaactt ctccggctct
    35041 gatatggtac tccagatgtt tgagaatcat caccttggac cagaaaacgt tgaacgccgt
    35101 ccggcccagt tcataaaaac ccagttcctg ttcggcgatt tcttggagtg aacgcggctg
    35161 ggcaagaaaa tccatgattt tctcatcccg gtccagggca atttgcacat aggcacgcag
    35221 atgtttttca atctcatcgt aaaacacctt gcgatgggag gtcaccagca cctcaggctg
    35281 aagcgcaatc agcttttgta cagaggctac cacttccccg aaatccgaat cgtatcctcc
    35341 gtaccaggga ccgcgcggcg agatgtccat atctccggaa aaaaggatct ttttctccgg
    35401 aaaatagaat ccatagtgac caacggaatg tccaggggta tgcagcgcca acaagcgggt
    35461 ggaacccacg acaatctcat ccccgtcttt aaagactccg cccagttcca cctgccggaa
    35521 tcccggcttg accagaacat catccgggag cggggtttgc atcgtcattt tttcgtcttt
    35581 tttactgccc atcaactctt cccagcgttg aaatccggca gtgatgtaga acttgttttc
    35641 atcctgaaag gcatattgtt cttccgtgcc ggccataatt tttgcctggg ggaaatagcc
    35701 cagaccgtgg gtatggtcaa agtgcgaatg gctcaacagg atcaagccga tttcttctct
    35761 gggaatctcc tggtacgcat tgcccccgct gccggcatcg atcaaggttt tgatttcatc
    35821 gtcgatataa atggaattgc aatatggaaa catcgccttg ccctgcggac gaataaattt
    35881 aatctttggg tccagatcaa ttatcattta cgttcatgct cctgctccat cgttactatt
    35941 attatactaa aacaaaaccc ggttcaaaac cgggttttac aaattccttt aattaaccgt
    36001 ccagtctgtc catcatttcc atgatcatat cgaagaaaac gttcatatct tctttttcat
    36061 taatgcccgc tttaaaacca acctccggga atcttcctcc gctttgaatc ttgacactga
    36121 tcatataggg tgcttgttcc aggtcatcct ccagactctc caaatcgatg tcttcccaat
    36181 catcttccag cgggatgatg tcttcgtctt ccagtttaaa gatcgaggaa tccttgagtt
    36241 cctcactatt gtcgaggatt tgtgaagcaa tccaaatgac atcagttatg ctcatattta
    36301 attcgttgct gagatggagt atctcattgg caactggtgt tatcttcagc aagtcagata
    36361 aatttaccgg gtccccttta ctccatttgt ttttcacacc catcacctcc gcatctattt
    36421 ggtgaacgaa cctcatcata ttccgggtga tcattttata taaagcacca cgtgcaaggt
    36481 cacatttcat tctactcatt atatcaaaat tatatgtaat gccaatgtga aaaaaacaat
    36541 tttgcccggc tggctgaccg gagggctgta aacgcttggc ggtacagcct gcagtttaaa
    36601 ttgtaaatca ttcttgataa aaaatctccc ggcaagttat gggatttttt cacattttta
    36661 ttccaaactg ttactttcta ttcatcatat tcttgctatt ctgcttttca ttttaacagg
    36721 ttaacatgtt gctatttgtc gtattttcgt tcttcattgt cgcaagcaat ttgtcccttc
    36781 ttaaaaaaaa cagcctgtat caaacagtgc atctaatcac tttaaatcca ggctggaagt
    36841 ctttttaaat tcaaatcccg tccaaattca cttaaataat cgccctaatt acagcacaaa
    36901 attctacatt gctatttgca tcagttgctg gcgtccttct tctcttaatt cttcaacgac
    36961 aggctgaatc tgcctgcgaa tttccgtctt gtgcatggcc agataatcct ccagcgcttg
    37021 cgtggatatc ccttggtcat gtctgcccaa acgaatcccc aaacaatcct gtccctgcag
    37081 atagaaaaac agttctgcct catgttggcc gttatctgcc gcaaagaccc ctttgccggt
    37141 ggccatattg tagtccgtca tgtgcaagcc gttaaatgat actaattgca tgattggttc
    37201 gccttctttc tcgttacgta aattttatga tttaatcata tccttatagg taaatttttg
    37261 caatccgatt ggattatttt gtattattta atactatatt tgtcgatttg catgcctgtt
    37321 gttacacctt tcacttggtt gggcattttc aactgaaaag taacggcaag aaaccgtcaa
    37381 cccattccaa caaaaggcaa tcgggtagga ggccacccat taattgagta gccgacctcc
    37441 cacagcaccg ta
//
LOCUS       DAOTHZ010000012        36574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1249_length_36574_cov_7.618473, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000012 DAOTHZ010000000
VERSION     DAOTHZ010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 36574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36574
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1249_length_36574_cov_7.618473"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="P5549_02730"
     CDS             complement(<1..264)
                     /locus_tag="P5549_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRW12019.1"
                     /translation="MHIPDGYLSPQTAVPFVALMVPIWGAAIKKVQSDLKKREVPVLA
                     IGAAFCFTIMMFNLPIPGGSSAHAVGAVLLAILLGPWPACIGIS"
     gene            complement(505..1005)
                     /locus_tag="P5549_02735"
     CDS             complement(505..1005)
                     /locus_tag="P5549_02735"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRW12020.1"
                     /translation="MKADAGAAYSALEAFAVNEGNVSQVHVHDHHGNKEINLGQEADG
                     LLQIYVHEQGTRELYAKVVMEIGHHHHHGLQPLGLPLEIIPTDYSHARMGESYEIQVM
                     QNGQLLVGAEVKVTYASTRSKDYPHRLTTNEEGKCKVFLSGRGNYLFSVAAQGISSTF
                     TLVKSF"
     gene            complement(1117..1308)
                     /locus_tag="P5549_02740"
                     /pseudo
     CDS             complement(1117..1308)
                     /locus_tag="P5549_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
     gene            complement(1301..1912)
                     /locus_tag="P5549_02745"
     CDS             complement(1301..1912)
                     /locus_tag="P5549_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HRW12021.1"
                     /translation="MCKDLPPLERAIQFHGHICPGLLMGVRVAEFAQKHLGVNQDIDE
                     ELLAIVETNSCGVDAIQSILGCTFGKGNLIFKDYGKNVYTIASREKNSAVRIAQVYRE
                     QKSPESIRYRQLNSKGHLTEEEEAEREELLACIFEQTMSAPFAELFNWQDVELDFPEK
                     AQIHATVQCAICGEGVMDSKTVTGPQGYVCPDCLQAKEASINV"
     gene            complement(1949..2383)
                     /locus_tag="P5549_02750"
     CDS             complement(1949..2383)
                     /locus_tag="P5549_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HRW12022.1"
                     /translation="MNIAVVDGQGGGIGRLIVEKLRQELGASVKIIGLGTNAVASSAM
                     LKAGANEGASGENAVIYNVRKVDCIVGSVAILAANAFAGELSPPMAEAIASAEAVKIL
                     IPLNRSGIRISGAAGEPLPIQVDHLIEQVQEFGRQMELLCRT"
     gene            complement(2582..2794)
                     /locus_tag="P5549_02755"
     CDS             complement(2582..2794)
                     /locus_tag="P5549_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRW12023.1"
                     /translation="MRLSARNNFKGKVVKIVPGAVNTEVHLQLAGGETIVSVITKESA
                     AGLGLEVGKEAYAVIKASSIMIGVDH"
     gene            complement(2909..3190)
                     /locus_tag="P5549_02760"
     CDS             complement(2909..3190)
                     /locus_tag="P5549_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HRW12024.1"
                     /translation="MGWKDKGTYFVEEIELAVDGLCERIRELVQEGNVRRLIIRKENG
                     DKLLEVPLTAGVAVGGVIAVIWPLIAAAAAVTGLMTRVRVQVIRKWDDS"
     gene            complement(3405..3992)
                     /gene="yedF"
                     /locus_tag="P5549_02765"
     CDS             complement(3405..3992)
                     /gene="yedF"
                     /locus_tag="P5549_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRW12025.1"
                     /translation="MREIVDARGLSCPQPVVLTKKALEREEVKEVLTIVDNPTALENV
                     ARMVKTLQLGSVVDEKEGQYYISIFKEEGLQGKNEKETLEGDTVILINSNVLGQGDNK
                     LGGILMKSFLYTLTQLEGEIKSVIFMNSGVLLTTAGSELIEHLQVLAEHGTEILSCGT
                     CLDFYNVNNKLAVGTVTNMYTITEKLLHAGRVLNF"
     gene            complement(4202..6067)
                     /locus_tag="P5549_02770"
     CDS             complement(4202..6067)
                     /locus_tag="P5549_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW12026.1"
                     /translation="MHKLTQNLPIWRDNDINWDKEFEKYLAENKTLAFMMLKNAKIFK
                     DKVILMQKNVKGEWESLTWTEFGERITALAKALIDKGIQPGDMCSVFARNCSEWAISD
                     LGILATRAVSVPIYATNSKEEAEYIINDAEVKVVFVGDQDQYDRADKIIANNQYLQFI
                     VAMQDNIKISGEKSEYLSKLIEKGKKLKNDKEFDDRLNNVHPDDKVTIIYTSGTTGNP
                     KGAVHTHRTFLTGIFPSLRYPEAGPDYTSLCILPLSHIFERLWSYGCMSIGVKIGYCP
                     NPADFMEYMVHYKPQFMTSVPRIWDKVYGTIHAGMKSAGGAKYKMFEWSKGVGIEAYR
                     SNHYGFKYKIADKLVFSKIRTMLGADHCNIYHIGGSAFAPDVNEFFQAIGINIAMGFG
                     LTEFFPVCVGFRDNAIPGYCGVLLSMCQARISDEGEVQLKGGMSMTEYYKNPEATKEI
                     FTEDGWFKTQDVGELHFVEKNGEKLGYIKITDRIKELIITAGGKNISPQQIESLLGNE
                     VMISQFVCIGENRKFLSALVVPNFLILEDYCKKNNIEYTSPEQIIQNPAIYKLYEKLI
                     ADKTKSLGQVEKIKKFGLLPQELTQEAGELTPTLKLKRKFIDQKYKPLIDKLYED"
     gene            6460..7014
                     /locus_tag="P5549_02775"
     CDS             6460..7014
                     /locus_tag="P5549_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRW12027.1"
                     /translation="MLELRINGFGGQGSVTLAHLLAQAALENGEQGQALPCFGVERRG
                     APVRAAVRMSPTTIFAHSQCELPDYVVVMSEKLTDAAISEGITEIGSVIINTPSKIAV
                     KQHDTWHVDADGIACEAGLFSADGPMINIPLFGAVCRVLNIPQQVMEQTLLNKWPGKN
                     GERNLAPANKAYNSVMKVSAALSA"
     gene            7044..7301
                     /locus_tag="P5549_02780"
     CDS             7044..7301
                     /locus_tag="P5549_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW12028.1"
                     /translation="MKSVTKDYRPISWPMQGAGGDTGSWRVHRPVVDMQKCTKCQLCW
                     IYCPEAVIDRQEIIIDYRYCKGCGVCALECPTGAIRMEKEE"
     gene            7306..8436
                     /locus_tag="P5549_02785"
     CDS             7306..8436
                     /locus_tag="P5549_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12029.1"
                     /translation="MKPAQVTMETGNYAAALAVKSARVDVVPGYPITPQTSIMEALSA
                     MVEKGELNARFIPVEGEHSAMAAAVAASAAGARVFTATSANGLLYMHEVLHMASGGRL
                     PVVMCNVNRGIFAPWTLWADHQDSMAQRDTGWIQYHCSSNQEVFNTILQAFRVAEQIN
                     IPVMVNFDGFAISHCLMPLTVPPQEDIDRFLPPHQPEWRLDPNHPSSFSNVTNAPEYA
                     PYRQMLSEDVMACLPLVKQSAEEYRAITGMWDGDTIDTYRLEDAEVVAFAINSMAAEL
                     RLSVDILREQGIKAGLLRPRLFLPFPAEDIINVLPQNAQVMVLDRNYNFGHPGGILAA
                     ELKSVLFGRRNDITVKNRVIGIGGIDLTRRFMADEILSMLEE"
     gene            8468..9406
                     /locus_tag="P5549_02790"
     CDS             8468..9406
                     /locus_tag="P5549_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRW12030.1"
                     /translation="MTTSIKKLPDTEQVLSGNGACGGCPATMALRIVGKALGPNAIML
                     LTPSCSVASMGMTPKLGYNWPCINICFATAGSSASGITHALEIMKEKGRLQGELPTVF
                     SWVGDGGTYDIGFQALSAAAERNDNIIHFCYNNEAYSNTGVQRSGATPRGSYTTTTPG
                     GKRQPKKNMALLMLEHNIPYVATATVAYPGDLYDKVVKAKSIPGMKYIEILAPCYLGW
                     QFDMPETVQMSRMAVNSGAWLLWEAENGQVNFNSPSTNIISGKKEPAPVNEYLKKQGR
                     FNRLLKGPDAAQRIAEIQADIDREIAFMRERSKITI"
     gene            9605..10309
                     /locus_tag="P5549_02795"
     CDS             9605..10309
                     /locus_tag="P5549_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRW12031.1"
                     /translation="MIFQPIKTKKIWEEIVEQLKAMITNGELKPGDKLPSERDLAESL
                     NVSRASVREALTTLEAFGILEIKPGEGTFIRQTSDAETFAPLTLLLTVEGNPEVQMME
                     VRRVLETEMAALAAQRATADNLDRIEESLCSMKAAKNTRTAVDADLRFHFAIAEATQN
                     TILLRLMNTIADLMHHSLRSNREKLYSDPPTGQRILREHDAIFQAIRNHDPQVARNAM
                     LEHINNIEKGIAEANK"
     gene            complement(10509..12299)
                     /locus_tag="P5549_02800"
     CDS             complement(10509..12299)
                     /locus_tag="P5549_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HRW12032.1"
                     /translation="MQRLLGAIKGIVQGLVKAVSRFPLTVIALVIATGLVWYMISLDK
                     NPDLFIQKLLFTCLLGAFLGVAVQFSCERFERLGRLRWLTYTGAALLAGVYYASLGAS
                     PSIEFDVQIRTLVAVFAMFSLALFIPTYKEGFDFDSLALIHFKSAFTAVLYSAVLSAG
                     CASIIAAVDILLFHIDESAYAYSMSFIWILFAVLYYLSLLPRFNSQDQNDREYAEGES
                     NYPRILEILVSYIAIPLVAVFSMVLIAYFFKILITLHWPAGQLGPMVLAYSAAGLIIY
                     ILAGRLENRSTSLYRMIFPKLLIPVVIMQLVSVGIRVQAFGITESRYYVALFGIFAIV
                     IAVFLSSKPKSRNGLIVVLAAAFAILSIVPPIDAFTVSRVSQTSRLEKMLQTQGILVD
                     GQLKPQADVPMELRIEATSILNYLQNRKYTAEIDWLPADFNTNKDMLKTFGFETAYPG
                     YPGEDKYFYAHLNMDDPIAIKGYDIMYNGHSYRGQGKNEIKPVYFTVDQVKYQLMVNR
                     VSPQETIVAVQDAQGKEMVATGLYDFAQSLQGITESSKGSLSSSEMTLIKEHNGAKLY
                     VLLQDVNVALGNGSDAGADYTFLVFFDAAE"
     gene            complement(12579..13538)
                     /locus_tag="P5549_02805"
     CDS             complement(12579..13538)
                     /locus_tag="P5549_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRW12033.1"
                     /translation="MIRRYVLFLCLWLLGCCFFMTSAVQAEQNAEPMKTERIRVDRTD
                     FPVLYCGQPRLEGEAIWMVQARLRELGYEIIPSGQYDEKTAEAVMMFQTAHRLQANGV
                     VTQLVWENLMYDGGSQPCLTSQEQPQKASVEIDLIKHRLTVFQNGDIIKEYPVGVGKS
                     STPSPLGEWKVVHKGVNWGNGFGTRWMGLNVPWGIYGIHGTNKPYSIGASMSHGCIRM
                     RNRDVEELYPLIPLGTRVRIVENGQIFPRNFSGHALIIKNSGQNVVYLQSRLKEKGIV
                     FDAADGRFGHMTELAVKYYQAWHGLEPTGIADEATYRSLGMIQ"
     gene            complement(13620..14507)
                     /gene="sdaAA"
                     /locus_tag="P5549_02810"
     CDS             complement(13620..14507)
                     /gene="sdaAA"
                     /locus_tag="P5549_02810"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRW12034.1"
                     /translation="MYQFSTAQELLAMCEREKAGIAEVALRSEMEASGQAREYIWSRM
                     EELYQVMQESIKNGLAPDRKTLGGLIGGNAYKLQQQMQKGCLSGQTVLKAASYALAVT
                     EVNASMGKIIAAPTGGASGTLPGVIAAFIEDRKLGDEMAVQALLVGAAIGKIIASQAT
                     LSGAEGGCQAECGSAAAMAAAAVVYLAGGTAEMSVDAAAMALKGLLGLVCDPVAGLVE
                     VPCSKRNAIGTAHAVLCADMALAGITSYIPFDEVVEAMSRVGRMMSPDLRETARGGCA
                     ATPTARAYVLKMNQLRTEQ"
     gene            complement(14524..15189)
                     /gene="sdaAB"
                     /locus_tag="P5549_02815"
     CDS             complement(14524..15189)
                     /gene="sdaAB"
                     /locus_tag="P5549_02815"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRW12035.1"
                     /translation="MAVSLFDVVGPVMIGPSSSHTAGAARLGNMARRICGHGFQKVEL
                     LLHGSFAHTGKGHGTEKALLGGVLGFKASDERIRDSFALAEQQGVTFSFAEIDLGEQH
                     PNSVKIIFHDGKTTCSIIGSSIGGGKILIKEIDGMALEFSGEYPTLITRHHDEPGVVA
                     KVSAILSQYGVNIAFLKVFRQYRAGEASMVIETDQPVEPEILAQLEAVEGINRVIFVD
                     LDE"
     gene            complement(15224..15793)
                     /locus_tag="P5549_02820"
     CDS             complement(15224..15793)
                     /locus_tag="P5549_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRW12036.1"
                     /translation="METRNCIKERRSMRKFTEQPVSDEQLQELLEAIRWSPSWANTQC
                     WEVVVIKDQARKEQLAGMLSEKNPAGKGVVQAPVVLVVCARLGVAGYEHGEGAEHVFA
                     TDKGDWFMFDTGIASQNICLAAYDMGLGTVHVGHINHHEIDQWLGLPEEVCSVEVIPV
                     GYPAKEGKAPPRKPLGDFVHFEKYGQKQS"
     gene            complement(15891..17693)
                     /locus_tag="P5549_02825"
     CDS             complement(15891..17693)
                     /locus_tag="P5549_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRW12037.1"
                     /translation="MDHKIKQSNRLIEQKSPYLLQHAYNPVDWFPWSEAAFTKAKQEN
                     KPVFLSIGYSTCHWCHVMAHESFEDQAVADLLNRHFVAVKVDREERPDIDYIYMMVCQ
                     TLTGHGGWPLTIIMDSEKRPFYAATYLPKESRGGMPGLLELLPRVARLWGEEKQALMQ
                     SGQQISQLIDMKPEKGEKQTVDLSLLSTAYHQFTRSFDNQWGGFGPPPKFPTPHNLLF
                     LLRYYETMKEDHALQMTEKTLQSMYRGGMYDHVGFGFARYSTDRVWLVPHFEKMLYDN
                     ALLAMAYLECYRLTNHAFYATVSRDIFAYVLRDMTGDAGAFFSAEDADSEGEEGRFYL
                     WSKDEVEQILGQKAERWAQTYDISAKGNFEGKNIPNLIHSANYYEARLGMEAERQLML
                     ACRAQRPRPLKDDKVLTAWNGLMIAALAQGARILKEDRYWQAACRAVDFINSHLRRDD
                     GRLWASYREGQAGHLAYAADYAYLIWGLLELYKTGRDENHLAMAQQFNQDMLRFFWDQ
                     EAGGLFFYGNDAETLLTRPKEGHDGAMPSANAVAAMNFLYLARLTGDEELEQKAGQTL
                     DCFAAQIAAQPTAHSYWLLMVNEKFFPQGRIVLS"
     gene            18212..18982
                     /locus_tag="P5549_02830"
     CDS             18212..18982
                     /locus_tag="P5549_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRW12038.1"
                     /translation="MIFRSNYRKLAIYIITFFILFSALAIPIKAEAASSVKVKKLASG
                     TKYETKLYVIKSGKAGPVVLIVGGVHGNEIAGYKAALKVCNLRPSRGTLLVIPKANVL
                     AIAEEKRVASGQLDLNRAFPSTKTGPARGILANSILKVIKDYDVDWVMDMHEGYDYAR
                     NPSTVSVGQSLIYYPSSSVKTIGSRIVKTLNKNISLQRKEFSLLKPPVKGSISRAAAV
                     TVGARSFIFETCSRESLATRINHQLKAANMLLDYLNMK"
     gene            19130..20008
                     /locus_tag="P5549_02835"
     CDS             19130..20008
                     /locus_tag="P5549_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016135026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRW12039.1"
                     /translation="MKYWGALCLALAASIWGSMYVASKYVLDYVPPFTLLWLRYALAG
                     IVLTIILLVTKKHKVASRDILLLAWIGFIGYFISIGAQFIGTKLADAHTGALMTSSSP
                     VFTFLLAWLLLEETITRRKLLALLLSMLGIIAVIGITGNEPINVGGSIALMVAAITWA
                     LLSVYVKKASTRYSSLTITTYAILFALLFTTPAAIGEMQHQTVRLFANPLVWTGTLYI
                     ALISTALAFFLWNKGMDLLEAGAGSLFLCFQPVVGSLLGWLLLDEVLKTSFFIGAAFI
                     ISALVIASLPPKKALP"
     gene            20095..21732
                     /locus_tag="P5549_02840"
     CDS             20095..21732
                     /locus_tag="P5549_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRW12040.1"
                     /translation="MKKIYVCLLMLLVVFILTAGVQAKLVPGDFIDLHGHWAQQDIQL
                     LAQLEIMQGTGTTPQGYRVFAPDSLVTRAQMAKVLTDTFHLDYGQLRFIKQPVASDFY
                     RDVDNNSWYADAVLMCAINEIFYTDYNFSPAHNLTRIEVANAIYRSFQAKKISVPMIL
                     MMPVYNDTQDLVQDDMNAMVFVSNTGIMKGDGQNFRPNDSLTRAELAAITSRCIELIA
                     LNENNHEQEYVMKSGQEFILSLASNPSTGYSWDFNKSFDDKLLELAVDKTYRADSPVN
                     ETMVGQGGRTYWKFKALQNGKTELELRYARPWESVQPGQTYKLTIHITDTDPVTPEDI
                     SIQTKAYNRLAEYMETNLRIPCLQGMPDEVLQAKLNDRFVEDALALEKILQADVQQYV
                     ANAQASGFPIHNFVLYSRFQPGYLNQHLLSLTIDYYQYTGGAHGITERRPYNVDLQSG
                     QDLTLKNLFVDNYDYASVINQEIKKQISNGEPVYFEGNRGFQEISDNQAFYLQEEALV
                     MVFQQYEIAPYAAGIPEFKIPLRLFGDKFRVDLLAAK"
     gene            21845..22033
                     /locus_tag="P5549_02845"
     CDS             21845..22033
                     /locus_tag="P5549_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRW12041.1"
                     /translation="MNLREALELAAKEEAKAMQNYTRLAGEAEDPETRLMLEQIAKEE
                     AGHLKRLTERLKAIKMLG"
     gene            complement(22076..23404)
                     /locus_tag="P5549_02850"
     CDS             complement(22076..23404)
                     /locus_tag="P5549_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12042.1"
                     /translation="MIVTTQLVWRFYYFMAEDIESLQKLTDIFHGHGLLLDTSNTFES
                     DRQTEGTMYNLDYPQGKLKILAVKPTHGNEHWQTALKELRTWEEEDPLAAMDIVEVLT
                     IIAGTEQREEAAKTVAAAQTGGDIYTLQKGRLSCLKRDRSKHQTIYLFSRDNNETDDL
                     SFLSRRLPLLHGGVLRLQALDFLLHDRLLSIGREKEEIQRGLIKVLHTKLVMNQATLM
                     AAEELENEIQTLAAAYGKLVEDQNMLVDGQKNFDAAMLALQRQINVEAFWQEPAAVFA
                     QLTEPYQQRRDEMNRLRDELNEVQEDYRAAMNVVQSKIEVMNSRTNIATQEQIKGLLE
                     VNTEMQKQGLVYQYAAGLIEFIVLAYYSHTLWSHLQHAAYTVIPSWIQFITVMLFSGN
                     VVWATHLIAEYNQGEHHVRTKLRIALFLFLLLFVFIVVGSILAAGHSTGH"
     gene            23648..24112
                     /locus_tag="P5549_02855"
     CDS             23648..24112
                     /locus_tag="P5549_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRW12043.1"
                     /translation="MSKKITLSKFVFDRLSKHFKDFTETQNKLLNDTEMVPAAERKTY
                     QEIFMQYLQHLERLLKEAQITDSEDNSLPCVTFGSVVELENLENKRINKIFVTYPTAN
                     PPVDKKIKVSLTSYNSPVGKALFLKKPGDTVEVNAPGGKFKYRIKSVDLMIS"
     gene            complement(24123..24593)
                     /locus_tag="P5549_02860"
     CDS             complement(24123..24593)
                     /locus_tag="P5549_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HRW12044.1"
                     /translation="MRKNKIVILGMGNPLFRDEGLGIHLIKRLMQELITDGVELVDGG
                     TDGLALLNVVEATDYLLVVDAVMGDNAPGTVCKLAVQDMPMQVSGHLSTHQTGFQEIL
                     ALAALRGRLPQHLVLLGIQPQCLDWGTQLSPPVARALPELTGLISEQLKEWLDC"
     gene            complement(24580..25254)
                     /gene="cybH"
                     /locus_tag="P5549_02865"
     CDS             complement(24580..25254)
                     /gene="cybH"
                     /locus_tag="P5549_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HRW12045.1"
                     /translation="MLKRAVYVWEAPIRLYHWINVVLIIALLITGLYIDKPFAFQTGE
                     PYANFIMGKMRLWHALTAWFFIANFLFRLYWAFAGNEHAQFKPWRKGFVADGMDSLKY
                     YLFLKKEHTVHTGHNVIAQLTYFFVLWIGSLFMILSGLAMQGEIAPGGFQERFFGWVI
                     PLFGMSSPVRMYHHLVAWLFVVFIAAHLYLVIRQEILDDDGTISSIFSGYKYVIDEAG
                     ASHEKE"
     gene            complement(25270..26982)
                     /locus_tag="P5549_02870"
     CDS             complement(25270..26982)
                     /locus_tag="P5549_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRW12046.1"
                     /translation="MAQRIVVDPITRIEGHLRIEAELEDGKIVNAFSSGTAFRGLERI
                     VQDRDPRDAWAFVQRICGVCTHIHAIASIQAVEDALDITVPKNAVLIRQIMTAAQFVQ
                     DHVIHFYHLHALDWIDVVSALKADPKAASALAQSLSNYARSSPGHFADVQKTFKSFVD
                     SGQLGIFANAYWGHPAYKLPPEVNLIALSHYLDALDWQKDIARIHTIFGGKNPHPNYL
                     VGGMACTINIDNDNTINMERLGLVFKEISKAKAFVEQVYLPDLFAIASFYKDWTYGGG
                     IDHFLVYGTLAEQNKNNNRDEIFPGGVILNRNLNEVFDVDPRDPKQVEEFVDHAWYEY
                     SSAPEGRHPWNGETRVKYSGPKPPYEQLDTQGKYSWIKTPLWKGKPVEVGPLARMLVG
                     YARGNDPIKSSVDEALQRLGLGQEALFSALGRTLARGLESKLMVDYLQKSYDLLLANI
                     KAGDSRVFDGSKWEPTSWPSSAQGVGFTEAPRGALGHWVKINNGKISLYQAVVPTTWN
                     AAPESKGGKGPYEAALIGTPLADPEQPLEILRIIHSFDPCIACAAHIIDLKSRQDLGE
                     CLFC"
     gene            complement(26982..28076)
                     /locus_tag="P5549_02875"
     CDS             complement(26982..28076)
                     /locus_tag="P5549_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRW12047.1"
                     /translation="MVRDSLYEAMIKRGYSRRDFMKICAILTAMMGLEASFVPKVAQA
                     LEDKPRLPVIYLHLQECTCCAESFIRTAHPLLADMLFNMISLDYMETLQAAAGHQAEA
                     AKAKTMQDYKDHYLVVVEGSIPLQDDGVYCTIGGRTARDILLEAARDSVAVIAFGSCA
                     TNACVQGAHPNPTGAVSVKEVVKDKPVIDVPGCPPIAEVIAGTIVHYLTFDRLPELTT
                     LGRPKAFFNHRIHDNCNRRAYFDAGLFVEQFDDEGAKQGWCLYKVGCKGPTTYNACAI
                     TQWNNGVSYPIKSGHPCIGCSEPGFYDDMPMYSRMAHIPGTAMPFNPDKIGLAVTGAT
                     VAGAAAHGLATGVLMRKKPQQKRVEKGGDE"
     gene            complement(28316..28615)
                     /locus_tag="P5549_02880"
     CDS             complement(28316..28615)
                     /locus_tag="P5549_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HRW12048.1"
                     /translation="MFEDKYLACKDCGSEFLFSASEQEFYAEKGFENEPGRCPSCRRA
                     RKQERNGGFGNSGRREERVMFKTVCAACGQETTVPFEPTGDRPVYCRDCFQARRR"
     gene            complement(28767..29321)
                     /locus_tag="P5549_02885"
     CDS             complement(28767..29321)
                     /locus_tag="P5549_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRW12049.1"
                     /translation="MLRFISKIYIKLAGWKIAGAIPPEIKKCVLLAVPHTSNYDFPIA
                     IAILRIMNVKIKYLIKKEWMKFPLGWFFKYTGAIAVDRSKSNNLIAAIVDIMNQRDEI
                     VMVIPPEGTRKLARKWKTGFYYVALGAKVPIVLSYLDYEKKTGGFGPAIYPTGNLVED
                     MHQIRDFYKDKVPRHPEKVSLDII"
     gene            complement(29431..30579)
                     /locus_tag="P5549_02890"
     CDS             complement(29431..30579)
                     /locus_tag="P5549_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRW12050.1"
                     /translation="MAEVAIVRCESYEQREVDAAVHRGLELIGGLRSFVKPGEKILLK
                     PNMLSADPPEKCVTTHPAVFKAAALALQKAGVQLSYGDSPGFGSPEGVARKSGIAAVA
                     AELGIPLADFHQGQEAVCAQALQNKKFTIAQGVLASEGLISLPKLKTHGLTRMTGCIK
                     NQFGCIPGFLKGEYHVKLPEVSHFAQMLVDLNVLLQPRLFIMDGIIAMEGNGPRGGRP
                     KKMNVLLFSSDPVALDAVVCRMVGVIPETVSTIQAGMKSGLGTCLEQDINLLGDVLEA
                     FADSTFDINRGSNHNQRRIPQSKLLREILLSKPCIIESGCVKCGVCIKVCPVIPKVLA
                     WPQGDQERPPVYDYDRCIRCFCCQELCPESTIIIKKTRLRRWVDKIHA"
     gene            complement(30598..32256)
                     /locus_tag="P5549_02895"
     CDS             complement(30598..32256)
                     /locus_tag="P5549_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRW12051.1"
                     /translation="MNSRWQLKYIKRRREIVGTFMRHGLGYFLQRFGFSERIALKSTR
                     TSVPGSVKNQYLLAVNLREALVELGPTFVKLGQLLSTRTDLLPPVFIGELEKLQDKVD
                     PISWEDLKQVLDREIGAVETVFADFNTQPLAAASIGQVHRARLHNGQEVIVKVQRPEI
                     EAKVRNDFEILKGLARISERRSSEASRLGILAMVEDYRRTLLRELDYEREARNTERVY
                     KNFADDERVIIPRVFKEYSTPRLLVQEYVEGIKLSDIEKIDEKGLDRRKISALGTEAF
                     LSQILLHGFFQADPHPGNIFVLDNEHIAFIDFGEIGYLSEVRLAYVGEMLIAIAKNDP
                     YRAMPVMRDMGILEQLDDEEDFTEAFAELVESLYTSSIGSLDMKRMRSEIMEFAYRFH
                     LKMPSYLTSLMKALITVEGLGKKLDPQFDFREVASPLAQKVYRERLKPSNLGKFLRSR
                     YYKEIEPLKKIPGNFNRLLQDTVDEKLRLNLDVNLSSKLYHKTGQMVNRLSFSLIISA
                     TLIGSAMIIQANHNALVERFAFLGIIGFGIALISMVAFFMGSLK"
     gene            complement(32447..34132)
                     /locus_tag="P5549_02900"
     CDS             complement(32447..34132)
                     /locus_tag="P5549_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12052.1"
                     /translation="MYKAMDKKIWEKNVPYWWPDRFHYGKDPLVKHLYDHSWRVPDKP
                     AIIFYGRVITFKELNDLVWRVAGGLQKMGMKKGDRLYIALQNCPQFIIAYYAASAIGA
                     TVITGSPLYKPGELRFVLNDCTPKVVLFEDDLFPVFAAIRDEVPSVEKVVVTSLGEYL
                     PEKPEILVSFDPGGKAGELPNTITWKELVESEPIQELADLDIKKDIAMLQYTSGTTGN
                     PKGAILTHFNVLANATNSAYGRPTYFDDIHVAVLPLFHVNGSNNSLNAPVLMGATIVL
                     LVRFDLETLYQAIEKYRISVWIGITTMNIAFLQHPKFKDYDLSSLTFAGTGGMPLPQA
                     LFNQYKEIFGFELQDGFGMSETMAYTICNSSGYSKVGSIGIPCPLVDVRIASLKDENI
                     DAEIGEEGELWQRGPSIAIGYWNNPEGTAEIFVQSDDSEFPWLKTGDIVKMDDDGFLW
                     LCGRTKEMIKVSGFSVYPAEVEEYLYHHPAILECCVIGVPHEYKGEEVKAFVVLRPDY
                     VGKVSEEEIIAWASKEMSAYKYPRLIEFRETLPKGGTGKIMRKVLKAEEAERK"
     gene            34520..34876
                     /locus_tag="P5549_02905"
     CDS             34520..34876
                     /locus_tag="P5549_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12053.1"
                     /translation="MQDTNEKNIFLKIVRASATNIVGFGLATALVSYLVRNTDVKTPL
                     MVLAGLIVFIMLVSLSSLMIFIYFTIKNIPETMRKAGGKSFGEVYKYILSALAVRLVE
                     AAICIVFIVHIYHLYF"
     gene            complement(34959..35381)
                     /locus_tag="P5549_02910"
     CDS             complement(34959..35381)
                     /locus_tag="P5549_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRW12054.1"
                     /translation="MYVLSIMLIVISNVFYNIAQKSTPQNANPMAALLVTYLTAISVT
                     LVAFLFTRSEKGFMQSFAALNWTSIVLGFAILGLELGFLLAYRAGWNISVGSLVANIL
                     LALMLIPVGILFYHESFNVYKVIGACFCIVGLILINKM"
     gene            complement(35492..36169)
                     /locus_tag="P5549_02915"
     CDS             complement(35492..36169)
                     /locus_tag="P5549_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRW12055.1"
                     /translation="MPTETSSQDKSGNPSSGTTSVNQHESKAASNPVESGSNPASEGN
                     STNSSNNQVSRGESAIDNSKYPLLSKINGSFGQFRYRELAGGRIEVDPQWVATNIVTI
                     TLPGLNRQVQVHKNAAENFITAFNYIKNGTAVVNGQEVALLSLIKTMDGTYVARHVNW
                     DSSRGLSNHSWGTAIDINASGHFGYVDPQQDQDDPNLILWEKAFKPAGFSWGNRYSDS
                     MHYELIK"
BASE COUNT         9586 a         9411 c         7851 g         9726 t
ORIGIN      
        1 ggatatcccg atacacgccg gccatggacc caacaagata gccagcagta ctgctccgac
       61 ggcatgggca gagctccctc cgggaatggg cagattaaac atcataatgg taaagcaaaa
      121 ggctgcaccg atcgccaata caggtacttc tcttttttta agatctgatt gtactttttt
      181 gatagccgcc ccccaaatcg ggaccataag cgccacgaaa ggaacagctg tctggggact
      241 taaataacca tccggaatat gcatttgtta ccctcctcaa tttatttgtt atgacagatc
      301 agtgatcttc atctctcaaa ataaaaagaa ggtatcgacc gattctgcgg tttatacctt
      361 catgatattt ttatttgtat gaaattagaa gaaccctggt catcaacaaa gcactttcat
      421 agcgccatta tatagactgc tattcgccat taatcaattt tttcctgcca atctgaaaaa
      481 gaataatacg gctgtcagca agtattaaaa actctttacc agcgtaaacg tactgctgat
      541 accctgcgcc gctacggaaa acaaataatt gccccgtccg ctcaaaaata ctttgcactt
      601 tccttcttcg ttggtagtca aacgatgcgg ataatctttg ctgcgggtac tggcataagt
      661 cactttgact tcggctccca ccagtagctg gccattttgc ataacctgga tctcataact
      721 ctcccccata cgagcatggc tatagtcggt ggggatgatt tccagcggca gccccaacgg
      781 ttgcaaaccg tgatggtggt gatgtcctat ttccattacc actttggcat aaagctcccg
      841 tgtcccctgc tcatgcacat agatctgcaa aagaccatca gcctcctgac ccaggtttat
      901 ttccttgttg ccatgatgat catgcacatg aacctggctg acattgcctt cattgacagc
      961 aaacgcctcc aaggctgaat acgccgcgcc ggcatccgct ttcatattgt agcctttgga
     1021 cagcttcaaa accacttcct cgccggcatg aaaatgaccg tgatcggtat ccggttccag
     1081 ccagatttcc ggatggcgat caaccacttc cggcttgtcg atctcctcca ggatgatgcg
     1141 atgatgcacc agttccattt ccttgattcg tgctctgacc accttgcgtt ccccaaaaat
     1201 actctccata aagatattgc catcttccgc cggaataatc cggtctacgc tttccataag
     1261 taattccttg tgaccggcgc gatcaagata gacgtttgat tcacacattg atggaagcct
     1321 cctttgcctg caaacaatct ggacatacat agccttgcgg tcctgtaact gttttgctgt
     1381 ccataacccc ttcgccacat attgcgcatt gcaccgtggc atggatctgc gccttttccg
     1441 ggaagtcgag ttccacgtcc tgccaattga agagttctgc aaagggtgca ctcatggtct
     1501 gctcaaaaat acacgccagc aactcttctc tttccgcttc ttcttcctcc gtcaggtgtc
     1561 ccttgctgtt taactggcga taacgaatag attccggact tttttgctca cgataaactt
     1621 gagcaatgcg aaccgcactg tttttctccc gactggcaat cgtatataca tttttgccgt
     1681 aatctttgaa aattaaattg cctttgccga acgtacagcc taaaatggac tgaatggcat
     1741 ccactccgca ggaattggtc tcaacaatcg ccagcagttc ctcgtcaata tcctggttta
     1801 cacccaaatg cttctgcgca aattcagcca cgcgtacgcc cattaacaag ccgggacaaa
     1861 tatgaccgtg aaactgaatc gctctctcca gcggaggcag atctttgcac atagtttccc
     1921 cacctttcca tgattatagt gatccatgtc atgttctgca gagcaattcc atttgtctgc
     1981 caaactcctg cacctgctca atgagatgat ccacctggat tggcagcggt tctcctgctg
     2041 ccccggatat tcttattccc gaacggttca gcgggatcaa tattttcact gcctccgctg
     2101 aagcaatggc ttctgccatg ggcggactaa gctcgccggc aaaggcattg gctgcgagaa
     2161 ttgccaccga gccaacgatg caatccacct tgcgcacgtt gtagataacg gcattctctc
     2221 cgctagcccc ttcattggca cccgctttga gcatggccga actggctacc gcattggtgc
     2281 ccagaccaat aatctttaca gatgcaccca gctcttgccg cagtttttcc acgatgagcc
     2341 tgccaatacc gcccccttgg ccatccacga ccgcaatatt cacagcctgg cctccttttc
     2401 tttttgaaac attatagcat aattcaaaaa ttgtgctcgt tagttttcag ggattttcct
     2461 ccctctttct cagtctttct cagtaccggt tctttcttgg ctcggattgc tcttctgaag
     2521 ccaattctcc aaaaaaaagc aagggactga tttcccttgc tgcaaaatat ccggtaattt
     2581 tttaatgatc cactccgatc ataatactgg aggccttgat aactgcatag gcttccttgc
     2641 cgacttccag gccgaggcct gcagcagact ccttggtaat gactgagaca atggtttctc
     2701 ctccggcaag ctgaagatgc acttccgtat tcaccgcacc gggtacgatt ttaacaacct
     2761 tgcctttaaa attgttgcgg gcactcaaac gcatgtccaa ttcctccttt tgttaaaatg
     2821 tttggccagc ataatggcct atttgtttac atttctatca taccatatga tctcgtctga
     2881 tctcgttatc cggagtcccc tttactcatt atgaatcgtc ccattttctg atgacttgaa
     2941 cccttacccg ggtcattaat ccagtcaccg ccgcagcagc ggcaatcagc ggccaaatca
     3001 cagcaatcac accgcctacg gctacgccgg cggtaagcgg tacttccagc aacttgtcac
     3061 cattctcctt gcgaataatc aagcggcgaa cgttgccttc ctgaacgagt tctctgatgc
     3121 gctcgcataa gccatccact gccagttcaa tctcctcaac aaaatacgtt cctttatctt
     3181 tccatcccat ccagacaccc tcccgtattt agagttgttt tcatttttcc cttatatcta
     3241 tagattatta ataaactggc cgttttggtt actgttgcaa tgaaatgtgg aagaattttg
     3301 ataagcaaag ctgatgtcaa tagggcaaag aagcggtcct gatggcagca ccctagtccc
     3361 cgacggttcc aacctgccca ggccctttaa ttacctaatg taaatcagaa attcaggact
     3421 cttccagcgt gcagaagttt ttcagtgatg gtgtacatat tggtcactgt tcccaccgcc
     3481 agtttattgt tgacattgta aaaatcaaga caggttccgc aggaaagaat ctccgttcca
     3541 tgctcggcca gaacctgcag gtgttcaatt aattcagatc ctgccgtggt gaggagaacc
     3601 ccgctgttca taaaaataac actcttgatc tcgccttcca actgggtcag ggtgtataag
     3661 aagcttttca tcaatattcc ccccagtttg ttatctccct gacccaacac attgctgttg
     3721 atgaggatta ctgtgtcacc ttccagcgtt tctttttcat tcttgccctg tagtccttcc
     3781 tccttgaaaa tactgatata gtactggcct tccttttcat cgacaacact tcccaattgc
     3841 agcgttttaa ccatccgtgc tacgttttcc agggcggtcg ggttgtcaac gatcgtcagg
     3901 acttccttta cctcttcccg ttccagcgcc tttttggtca gaaccaccgg ttgtggacag
     3961 cttagcccgc gggcatcgac gatttctctc atgtgtagac tcaactcctc tgttggttag
     4021 ctccagcatt aacaatcgta tcttaccaag ccttatttta tcatatgtat ttaatatcat
     4081 agctattaaa gtgagttata taaccagtct gccggcgcgt tataaaaaat gccccctgaa
     4141 tgagcacact aaaaaagcac cccgtggttg ataccggggt gcctggtagc gggaacctcg
     4201 tttagtcttc gtagagtttg tcaatcagcg gtttgtactt ctgatcgata aattttcgtt
     4261 tcagcttcaa cgtcggagtc agttctccgg cctcctgggt aagttcctgg ggaagcagcc
     4321 caaatttctt gattttttcc acctgcccga ggctcttggt tttatccgca attagctttt
     4381 catatagttt atagatggca ggattttgaa taatctgttc cggtgatgta tactcaatat
     4441 tattcttttt acaataatct tccaggataa ggaagtttgg taccaccaat gcggaaagga
     4501 atttccgatt ttctccgatg caaacaaatt gtgagatcat aacttcattg cccagcagcg
     4561 attcgatctg ctgcggggaa atgtttttgc cgccggcggt gatgatgagt tccttgatac
     4621 ggtcagtaat tttaatatag cctagttttt cgccattctt ttctacaaaa tgcaattcac
     4681 ctacatcctg ggtcttgaac catccgtcct cagtaaatat ttctttggtc gcttcaggat
     4741 ttttgtaata ctcggtcatg gacatgccgc ctttgagctg cacttcccct tcatcggaga
     4801 tccgcgcctg gcacatggac agcaacacgc cgcaataacc gggaatggca ttgtccctga
     4861 acccgacgca aaccgggaaa aactcggtta atccaaagcc catggctata ttgataccga
     4921 ttgcctggaa gaattcattt acatcgggag caaaagcaga accgccgata tgatagatat
     4981 tgcaatgatc cgctcccaac atggtgcgaa tcttgctgaa gaccaatttg tcagcaattt
     5041 tatatttaaa tccgtagtgg ttgctgcgat aagcttcaat gcctacgcct tttgaccatt
     5101 caaacatttt gtacttcgca ccgccggcgc ttttcatacc ggcatggatg gtgccgtaaa
     5161 ctttgtccca gatcctcggc acactggtca tgaactgcgg tttataatga accatatatt
     5221 ccataaaatc agccgggttg ggacaataac ctattttcac cccaatgctc atacatccgt
     5281 aggaccagag tctttcaaag atatgggaaa gcggcaaaat gcaaagcgag gtataatcgg
     5341 gtccggcttc aggatagcgc aatgaaggaa atatgccggt caggaaagtt ctgtgggtat
     5401 gaacagcacc tttggggttc ccggtggttc ctgaagtata gatgatggta actttatcat
     5461 cggggtgaac attgttgaga cgatcatcaa attccttatc attttttaat tttttaccct
     5521 tttcaataag cttgctaaga tactcgctct tttcgccgga tattttgata ttgtcctgca
     5581 tggcgacgat aaattgcagg tattggttat tggcgataat cttgtcagcc cggtcatact
     5641 gatcctgatc gccgacaaat accaccttga cttccgcatc attaataata tattcggctt
     5701 cttccttgga gttggtcgca taaatcggta cagataccgc tctggttgcc agaataccaa
     5761 gatctgatat tgcccattca gagcaattgc gtgcaaaaac tgaacacata tccccaggct
     5821 gtatgccctt atcaatcaac gctttagcca atgccgtgat tcgctcacca aattctgtcc
     5881 aggtcagtga ttcccattca cctttgacat ttttctgcat aagtatgact ttgtctttga
     5941 aaatcttggc attcttcagc atcataaaag ccagggtctt gttctctgcc aaatactttt
     6001 cgaattcctt gtcccagttg atgtcgttat cacgccagat cggtaaattt tgtgtcaatt
     6061 tgtgcatgca agctcctcct tataatttta tatgactata attattatcc ttcgtaatat
     6121 ccgaagcgtc aacatattaa tttgatttat aagcgcattg ttgccgtttt tttggcagcc
     6181 ttaataaaat ttcaaattaa tttagcaatc atagatcccc acaacattat caaataatgt
     6241 cttacataat tttttaaaaa aagaaggaat tatttagttt cattgcgaat aataatctgg
     6301 tatactggtc agaccaccag aacatcgagt cagttggcaa cacagccagt tgacaaaacc
     6361 cgtcttcgag gaaaacatta ccgtctgatg agcttggatt ggtcaagttg cagataatat
     6421 gtgcctcaaa ggatccaggg aagaatggag gaacaaaata tgttggaact tagaattaac
     6481 ggttttggcg gacagggttc cgttaccctg gctcatctgc tggcccaggc cgcactggaa
     6541 aacggtgagc aagggcaggc actgccttgt ttcggggtcg aacgccgcgg tgcaccggta
     6601 cgggcagcgg tgcgtatgtc tcctaccaca atttttgccc acagtcaatg tgaactgcct
     6661 gattatgtgg tggttatgag tgaaaaactc accgatgccg caatcagcga agggattact
     6721 gagattggtt cggtaatcat caatacgccc agtaaaatcg ccgtcaaaca acatgacacc
     6781 tggcatgttg atgccgatgg catcgcatgt gaagccgggc ttttctccgc tgacgggccc
     6841 atgatcaata tccctctgtt tggtgcggtt tgccgggtgc tgaacattcc gcagcaagtt
     6901 atggaacaaa cgctgctgaa caaatggccg ggcaaaaacg gcgagcgcaa cctggctccc
     6961 gccaacaagg cctataactc ggtaatgaaa gttagtgctg cattaagcgc ttaatatttg
     7021 gattgacgaa aggaatggaa taaatgaagt ccgtcacgaa agactaccgg ccgatttcct
     7081 ggccaatgca aggagctgga ggtgacaccg gttcctggcg ggtgcaccgg ccggttgttg
     7141 atatgcaaaa atgcaccaaa tgtcagctat gctggatata ttgtccggaa gcagtcattg
     7201 accgtcaaga aattatcatc gactaccgct actgcaaagg atgcggtgtc tgtgccctgg
     7261 aatgtcccac gggcgcaatt cgtatggaaa aggaggagta accgtatgaa accagcgcaa
     7321 gttacaatgg aaaccggtaa ttatgctgcc gctctggctg taaaaagcgc acgtgtagat
     7381 gtcgtgcccg gctatccgat tactcctcag accagtatta tggaagcgtt gtctgccatg
     7441 gttgaaaaag gcgagttgaa tgcccgattc atcccggtag aaggtgaaca cagcgccatg
     7501 gcggcggctg tcgcagcttc tgctgccggt gcccgcgttt tcacggccac ttcagccaat
     7561 ggcttgctat atatgcacga agttctacat atggcctccg gaggacgttt gccggtggtg
     7621 atgtgcaacg tgaaccgtgg tatttttgct ccctggactt tgtgggcaga tcatcaagat
     7681 tccatggcgc agcgtgacac cggttggatc caatatcact gttcatccaa ccaagaagta
     7741 ttcaacacca ttttacaggc ttttcgggtg gctgaacaaa tcaacatccc agtgatggtc
     7801 aactttgatg gttttgccat ctcccattgt ctgatgcctc ttaccgtccc tccccaggag
     7861 gatatcgacc gtttcctgcc tccgcaccag ccggaatggc ggcttgaccc caatcatccc
     7921 tcttccttca gcaatgtgac caatgcgcct gaatatgctc cataccgcca gatgctgtcc
     7981 gaggatgtca tggcctgtct gcccctggtc aaacaatcgg cagaagaata tcgtgctatc
     8041 actggcatgt gggatggcga tacgattgac acctatcggc tggaagatgc cgaggtggtt
     8101 gcttttgcaa tcaacagcat ggcagcggaa ttaaggctct ccgttgacat tcttcgtgaa
     8161 cagggtatca aagccggcct tttaaggcca cgcctgtttt tacccttccc ggccgaggat
     8221 attatcaacg tcctgccgca aaatgcacag gtgatggtac tggatcgcaa ttataacttc
     8281 ggccatcccg gcggtattct ggcagcggaa ttaaaaagcg tactctttgg ccgacgcaat
     8341 gatatcaccg tcaaaaaccg ggtaataggt atcgggggca ttgatctgac ccgccgtttt
     8401 atggccgacg agatcctgtc catgctggaa gaatagcagc aaggaccacg aaaggaatgg
     8461 atggaatatg actaccagta taaaaaaatt gccggataca gaacaagtat taagcggcaa
     8521 cggggcttgc ggcggatgcc ctgccacgat ggcgctgcgt attgtgggaa aggctttggg
     8581 ccccaatgcc atcatgttgt tgaccccttc ctgctcggtc gccagtatgg gcatgacacc
     8641 taaactgggt tacaactggc catgcataaa tatttgtttt gcaaccgccg gttcctcggc
     8701 ctcaggtata acccatgcac tggaaataat gaaagaaaaa ggccgcctgc aaggcgagct
     8761 gcctaccgta ttcagttggg taggagacgg cgggacatat gacataggct ttcaggcact
     8821 ttctgcagct gcagaacgca atgacaatat cattcacttt tgctataaca acgaagcgta
     8881 cagcaatacc ggcgtacagc gcagcggtgc aacgccgcgc ggcagttaca ccaccactac
     8941 gccgggcggc aagcgtcagc ccaagaagaa tatggcctta ctgatgctgg aacataacat
     9001 tccttatgta gctaccgcta ctgtagccta ccccggcgat ttgtatgaca aggtggttaa
     9061 agccaaaagc atccctggca tgaaatatat tgaaattctg gccccctgct atttgggatg
     9121 gcagtttgac atgccggaaa ccgtacagat gagtcgtatg gcagtcaaca gcggcgcatg
     9181 gctgttatgg gaagcagaga atggccaagt caatttcaac agccctagta ccaatatcat
     9241 cagcggaaaa aaagaacccg cgccggtgaa tgaatatctg aaaaagcaag gtcgcttcaa
     9301 tcgcctgctt aaagggcctg acgctgccca acgcatcgca gagattcaag ccgacatcga
     9361 tcgcgaaatc gcatttatgc gtgaacgttc caagatcacg atctagtctt gatgggtttg
     9421 tctgtacaaa ggcgagctgg caatcacagt gctttcattt agccttgcat ggtcatgacc
     9481 acctgcgctc accaggatat acatgtcatt cccacaatgg tataataccg gaaggacagg
     9541 aatgcgtcct gcagttcgtc tgcaaaagca aaattcctac ccgagcagaa aagcaggtgg
     9601 tattttgatc ttccaaccta ttaaaaccaa gaagatttgg gaagaaattg tagaacaatt
     9661 aaaagccatg attaccaatg gcgagcttaa accgggggat aaattaccct ccgaacgcga
     9721 tctggctgaa tcattaaatg tcagccgggc ctctgtccgg gaagccctca caacacttga
     9781 agcgttcggc attctggaaa tcaaaccggg tgagggaacc ttcatcaggc agaccagcga
     9841 tgcagaaact tttgccccgc tgacgttgtt attgacggtg gaaggcaatc cggaagtgca
     9901 aatgatggaa gtcagaaggg tgctggaaac tgaaatggca gcgctggcag cccaaagagc
     9961 aactgcggat aacctggatc gaattgaaga atccttgtgt tcgatgaaag cagctaaaaa
    10021 cacccgcact gcagtagatg cagatttgcg ttttcatttc gcgattgcgg aagcaaccca
    10081 gaataccatc ttgctgcgac tgatgaatac gattgcggat ttaatgcatc attccctgcg
    10141 ctccaatcgg gagaaactct attccgaccc tcctacgggt cagcggattc tcagggaaca
    10201 tgatgccatt tttcaagcca ttcgcaatca cgacccgcaa gttgccagaa acgctatgct
    10261 ggaacacatt aacaatattg aaaaagggat tgcggaagcg aataaataag tatgaaacgg
    10321 ggccggcatt gtatgtcagc cccgttgcca ttgattcatt ttcccgtcta gctgcagatg
    10381 agtcactttt acatttcata cccggttctg tatccatgtc cattccaaac tgttccctgt
    10441 caattccccc gcgggccaaa taaactgttc ttccagccat gtgccttcag tgatgggctg
    10501 aggtgagatt attccgcagc atcgaagaat accagaaagg tataatcggc cccagcgtcc
    10561 gaaccgttgc caagagcaac attcacatcc tgcaacaaga cgtatagttt ggccccgtta
    10621 tgctctttta tcagcgtcat ctccgacgaa ctaagcgagc ctttggacga ttcggtgata
    10681 ccctgcaaag actgggcaaa atcgtacaga cccgtcgcca ccatttcctt gccctgcgca
    10741 tcttgcaccg ccacaatggt ttcctgcggc gaaacccggt tcaccatcag ttgatacttt
    10801 acctgatcaa ccgtaaaata aaccggcttt atctcatttt ttccctgccc ccgataggaa
    10861 tgaccattgt acatgatgtc gtagcccttg atggcaatgg gatcgtccat attgagatgg
    10921 gcataaaaat atttatcttc acctggatat cccggataag ccgtttcaaa accaaaggtt
    10981 ttaagcatgt ctttgtttgt attaaaatca gccggcagcc aatcaatctc cgcagtatac
    11041 ttgcggttct gcaggtaatt gagaatactg gtagcttcaa ttctcaattc cattggaacg
    11101 tctgcctggg gcttgagctg cccgtcaacc agaatgccct gggtttgcag cattttttcc
    11161 aaacggctgg tctgactgac ccgggatacg gtaaaagcgt cgattggcgg cactattgac
    11221 aaaatcgcaa aggcggctgc caagaccaca atcaggccgt ttctgctttt tggtttagaa
    11281 ctcaggaaaa ccgctatgac aatggcaaag atgccgaata aagcaacgta atagcgggac
    11341 tcagtaatgc caaatgcctg cacccgaatc ccaaccgaaa ccaattgcat aatgaccacc
    11401 gggatgagca acttgggaaa tatcatgcgg tataaagatg tggatcgatt ttccaaccgc
    11461 ccggccagaa tataaatgat cagaccggca gctgaatagg caaggaccat cgggccaagc
    11521 tgcccggcgg gccaatgcag ggtgatcagg attttgaaaa aataagcaat tagaaccatg
    11581 gaaaaaacgg cgaccaaagg aatggcaata taggacacca atatctccaa aatacgtgga
    11641 taattgctct ccccctcggc atactcgcga tcattctgat cttgtgagtt aaagcggggc
    11701 agcaaagaca aatagtaaag caccgcaaat agaatccaga tgaagctcat actgtaagca
    11761 tatgcgctct cgtcaatatg aaacagcaga atgtcaacag ccgcgatgat actggcgcag
    11821 ccggccgata ataccgctga gtagagcacc gcagtaaagg ctgacttgaa atggatcagc
    11881 gccaggctgt caaaatcgaa tccctccttg taagtcggga taaacaaagc cagactgaac
    11941 atggcaaaga ccgccaccaa agttctaatc tggacatcaa attcaatgct cggcgatgcg
    12001 cccaagcttg cataatagac tcctgccagc aaagctgccc cagtatatgt cagccatctc
    12061 agccgaccca gacgttcgaa tctctcacag ctgaactgaa cagcaacacc caaaaaagct
    12121 cccagcaaac aagtaaacaa aagtttctga atgaagagat ccggattctt gtccagagaa
    12181 atcatatacc acaccagacc ggtggcaatg accaaagcaa taaccgtcaa gggaaaacga
    12241 gataccgctt ttacaagtcc ttggacaatg cctttgattg ccccgagcaa acgctgcata
    12301 atcatatcct cctttttaaa tcttcccgac tggacaacct ataactgcaa ttacgttacc
    12361 gtcggccaaa ggcttaccag gtcagacaca aaagcactcc ttttaaaact atgctatcag
    12421 tttaaaggaa ccggaaagca atcacaatca acaataaaag acgaaccaaa ttttctttga
    12481 tccgtctctt ccttgccaaa taaacaatta ttcccaacca tcttgaacca tcccctctta
    12541 ccgggtgact gtctttatag ctgaacggtt ggtaggcctt attgaatcat ccccagggag
    12601 cgataggtgg cttcatccgc aatgccggtc ggctccagtc catgccaggc ctgataatac
    12661 ttcactgcca gttctgtcat atgaccaaag cgtccgtcgg cagcatcgaa aacaattcct
    12721 ttttccttga gccggctctg cagatatacg acattctggc cgctgttttt gattattaaa
    12781 gcgtggccgg aaaaattccg gggaaaaatt tgaccgtttt ccacgatcct gacccgggtt
    12841 ccaagcggga tcagcgggta aagctcttcg acatcacggt tcctcatgcg aatacagcca
    12901 tgcgacatgg aagcacctat ggaatagggt ttgttggtgc catgaattcc atagattccc
    12961 cagggtacat tcagtcccat ccagcgggtg ccaaagccgt tgccccagtt gacccccttg
    13021 tgtaccactt tccattcacc cagcggtgaa ggagtggagg atttgcccac ccctaccggg
    13081 tattccttga tgatatcacc gttttgaaaa accgtcagac ggtgcttgat taaatcaatt
    13141 tcgacgcttg ccttctgcgg ttgttcctga ctggtcaaac agggttgact gcctccatcg
    13201 tacattaaat tctcccaaac gagttgggtg accactccgt tcgcctgcag gcgatgggcg
    13261 gtttgaaaca tcataacagc ctccgcagtt ttttcatcat actgaccgct ggggattatt
    13321 tcatagccaa gttcccgcaa gcgggcctga accatccaaa tcgcttcgcc ttccagcctc
    13381 ggttgcccgc aatacaaaac cggaaaatcc gttcggtcaa cgcggatcct ctctgttttc
    13441 atcggttcag cattctgttc agcctggacg gcagaagtca tgaaaaaaca acaacccagc
    13501 aaccacaggc aaagaaaaag aacatatcta cgtatcacag atctaccccc taaaaatgtt
    13561 ctctgttagt agatatattc cccgaaaacg aaaaaaagca tagcaagtga ataaaaatat
    13621 tattgctctg ttcgcaactg attcatcttc agcacatagg cacgggcggt tggggttgcg
    13681 gcacatcccc cccgggctgt ttcccgcaga tccggggaca tcattcttcc tacccgggac
    13741 atcgcctcca ccacctcatc aaaaggaata tagctggtga tcccggccag cgccatatca
    13801 gcacagagta cggcatgggc agtgccaatg gcgttgcgtt tggagcaggg tacctccacc
    13861 agaccggcca ccggatcaca taccaagccc agcagacctt tcaatgccat cgcagctgca
    13921 tccacggaca tttctgccgt tccacctgcc aaatagacta ccgctgccgc cgccatggct
    13981 gctgccgagc cgcattccgc ctggcatcct ccttctgccc ctgaaagcgt cgcctgactg
    14041 gcaatgatct tgccgatcgc cgctcccacc agcaaagctt gtaccgccat ttcatctccg
    14101 agtttcctgt cttcaataaa agcagcaatc actccaggca gcgtcccgct ggcgcctccc
    14161 gtcggcgcgg caatgatctt gcccatggaa gcattgactt cggtcaccgc cagcgcatag
    14221 ctggccgcct tgagcaccgt ctgcccgctg agacaccctt tttgcatttg ctgctgcaat
    14281 ttataggcat tgccgccgat caagccgccc aaggtcttgc ggtcaggcgc cagtccgttt
    14341 ttgatacttt cctgcataac ttgatatagt tcttccatcc tggaccagat gtattccctg
    14401 gcctggccgg aagcttccat ttcagaacga agggcgactt ctgcaatacc agccttttcc
    14461 ctctcgcaca tggccagcaa ttcctgggca gttgagaact gatacatgcc atccctccgt
    14521 ttcctattca tccaggtcaa caaatatgac ccgatttatt ccttcaactg cttccagttg
    14581 cgccaggatc tccggttcta caggttggtc ggtttcaatt accatcgaag cttcaccggc
    14641 ccgatactga cgaaaaactt tgagaaaagc gatattgacc ccgtattgtg acaagattgc
    14701 gctgaccttg gccacgactc ccggttcgtc atgatgccgg gttataaggg ttgggtattc
    14761 gccgctgaat tccagtgcca tgccgtcaat ctccttgatc aggatcttcc cgccgccaat
    14821 cgatgaaccg atgatactgc aagtcgtttt cccgtcatgg aatatgattt ttacactgtt
    14881 gggatgttgt tcccccagat caatttcagc aaaagaaaac gttactcctt gttgctccgc
    14941 caaggcaaaa gaatccctga tcctctcatc actggcctta aagcccagta caccgcccag
    15001 caacgccttt tcagtcccat ggcctttgcc ggtatgagca aaggatccgt gcaaaagtaa
    15061 ctccactttt tgaaaaccat gcccgcaaat gcgtcgggcc atgttcccca agcgagctgc
    15121 cccggccgta tgtgaactgg atggccctat catgaccggc cctaccacat caaacagact
    15181 taccgccatg cccggctccc cccatatact cgttcaactc tcatcaactc tgcttctgcc
    15241 cgtacttctc aaaatggacg aaatccccca acggtttgcg aggtggtgcc ttgccttctt
    15301 tggccggata accaaccggg atgacctcca ccgaacaaac ctcttccggc agacccagcc
    15361 attgatcaat ttcatgatgg tttatatgcc caacatgaac cgtacccaat cccatatcat
    15421 aggcagccag acaaatgttc tgactggcaa tgccggtgtc aaacataaac caatcgccct
    15481 tgtccgtggc aaaaacatgt tcagcacctt ctccatgttc ataaccagcc acacccaacc
    15541 gggcacaaac caccaaaacg acgggagcct ggactacgcc tttgccagcc ggattctttt
    15601 ctgacagcat gcctgccagc tgctctttgc gagcttggtc ttttataacc accacttccc
    15661 agcactgggt attggcccag gaaggtgacc agcgaatcgc ttccaacagc tcctgcagtt
    15721 gttcgtcact gaccggctgt tctgtaaatt tgcgcatgct gcggcgctct tttatacaat
    15781 ttctggtttc caaacaaatt cctcctcaca taattgaatg ggcctgttta gcctcattat
    15841 agcacaccaa cgaatctctg ccgtcaacaa tttgataaat ttttccaatt ttatgacaac
    15901 acgatcctgc cttgcgggaa gaatttttcg ttaaccatta gaagccaata ggaatgtgca
    15961 gttggctgcg ctgcaatctg agcagcgaaa caatccaacg tttgtccggc tttctgttcc
    16021 aactcctcgt cccccgtcag ccgggccaaa tagaggaaat tcatcgctgc gactgcattg
    16081 gcagaaggca tcgcaccgtc atggccctcc ttgggacgtg tcagcagggt ctcggcatca
    16141 ttcccataga aaaacaatcc gccagcttcc tgatcccaga aaaagcgcag catatcctga
    16201 ttgaattgtt gtgccatggc cagatggttt tcatccctgc cggttttata caactccagc
    16261 agaccccaaa tcaaataggc atagtctgcc gcataagcca gatgccccgc ctgcccctcg
    16321 cgatagcttg cccatagcct gccatcgtca cgacgcagat gagaattgat aaaatccact
    16381 gcccggcagg ctgcctgcca atacctgtcc tcttttaata tccttgctcc ttgggcaagc
    16441 gccgcaatca tcaatccatt ccatgccgtc agcaccttgt catcctttag cggtcgtggt
    16501 ctttgcgccc ggcaagccag cattaactgc ctttctgcct ccatccccag acgcgcttca
    16561 taataattgg cagagtgaat cagattgggg atatttttgc cttcaaaatt cccttttgca
    16621 ctaatatcgt aagtctgcgc ccatcgttcg gccttttgcc ctaaaatctg ctcgacttca
    16681 tctttgctcc acagataaaa cctgccctct tccccttctg aatccgcgtc ctccgccgag
    16741 aaaaacgccc ctgcatctcc agtcatatcg cgcaggacat aagcaaagat atcccggctg
    16801 accgtagcat aaaaggcatg gttggtaagc cggtagcact ccaaataagc catggcaagc
    16861 aaagcattgt cataaagcat tttttcaaaa tgcggtacca gccagacacg atcggtagaa
    16921 tagcgagcaa aaccaaatcc gacatggtca tacatgccgc cccgatacat gctttgcaaa
    16981 gttttttccg tcatctgcag ggcatggtct tccttcatgg tttcataata gcgcagcaaa
    17041 aacagcaggt tatgcggcgt tgggaacttg ggcggcgggc cgaaccctcc ccactgattg
    17101 tcaaagcttc gggtaaattg gtgataggcg gtgctcagca aagacaaatc aaccgtctgt
    17161 ttttcaccct tttccggctt catgtcaatc aactgactga tctgctgccc tgattgcatt
    17221 aatgcttgct tttcttcccc ccacaaacgg gcaacccggg gcaacaattc caacaaaccc
    17281 ggcatccctc cccgactctc tttgggcaaa taggtagccg cataaaaagg acgtttttca
    17341 gaatccataa tgatggtcag cggccagccg ccatgaccgg tcagggtttg acacaccatc
    17401 atatagatat agtcaatatc ggggcgttcc tcccggtcaa ccttcacggc aacaaaatgc
    17461 cggtttaata aatcggctac tgcctggtct tcaaaagatt cgtgcgccat cacatggcac
    17521 cagtgacagg tagagtaacc aatagaaagg aataccggtt tgttttcctg tttggcttta
    17581 gtaaaagctg cttcgctcca gggaaaccaa tcgactggat tataggcatg ttgcagcaaa
    17641 taagggcttt tttgctctat taaccgattc gattgcttaa ttttatgatc caaattcaaa
    17701 tcacctatgt cattttttcg ttttcacatg tataaatcga tcctaccgta tattattggt
    17761 gatttccatc ataattaacc ttccatatca cagattatca ttctgccaac ccatctttta
    17821 tcgaagccct gatgctcccg ggtattcttc atttctttct ttttctcatt cgagagcctg
    17881 tttctttatg ctagccattc cgctctcttc gctgccggct gaaagagatc aaaactgtct
    17941 cttctgaaac attttcttta attatatcag gtaatttatg gcttttacgg gtttttcata
    18001 gttttataac cttatttttg cattatccaa acggggatgg aagttcgccc aattaccatc
    18061 atcaccgcat cagcaaccag tctttttgca atttggacaa gtcattaatt accactgaat
    18121 ccattaactt ggttgtctta taatgttgaa aaagaagacc gggaagcatt aaggattttt
    18181 cattcatttt aatcattgaa agggtgatag cttgattttt cgcagcaatt ataggaaact
    18241 cgcaatatac ataatcacat tcttcatact cttctctgcc ctggcgattc ccataaaggc
    18301 agaagcagct tcttcggtaa aggtaaaaaa actagcttcc ggcaccaaat atgaaaccaa
    18361 attgtatgta attaaaagcg gcaaggctgg tccggtagtg ttgatcgtcg gcggcgtaca
    18421 cggcaatgaa atagctggct acaaagccgc ccttaaagtc tgtaatctcc gccccagcag
    18481 gggcacattg ctggttatcc ccaaagccaa tgtgctagcc attgcagagg aaaaaagggt
    18541 ggctagcggg cagttagacc ttaaccgagc ctttcccagc accaaaaccg gcccggcccg
    18601 cggcattttg gccaattcaa ttttaaaagt catcaaagat tacgatgtcg actgggtgat
    18661 ggacatgcat gagggctatg attatgccag gaatccttct accgtttcag taggacaatc
    18721 tcttatatat tacccctctt catcggtaaa aaccatcggc agtcggattg ttaaaacctt
    18781 gaataaaaac ataagtttgc aacgcaagga attctccctg ctcaaacccc cagtcaaagg
    18841 aagtataagc cgggctgctg cagtcaccgt aggcgcccgc agctttatct ttgaaacctg
    18901 ttctcgggag agtcttgcaa cccgcataaa tcatcaacta aaagctgcca acatgttgct
    18961 tgactatctg aatatgaaat gatacctggc ggaggtttgc ttatgccggc ctccgccccg
    19021 ctcccctcat tgttcctttc gttggctgcc tttgcttaag aatttgtcct cacgcaccct
    19081 ccaatctgtt ataatagcaa tgtttatttg taaattggag gaaacacccg tgaaatattg
    19141 gggagcccta tgtttggcct tggcagcctc gatctggggc agcatgtatg tcgccagcaa
    19201 atatgtgctt gattatgtac cgccttttac cctgctatgg ttgcgttatg cgctagccgg
    19261 gatcgttttg acaataatcc tgctggtaac gaaaaaacac aaagttgcca gccgggatat
    19321 cctgttactg gcctggattg gctttattgg ctattttatt tcgatcgggg cgcagttcat
    19381 tggaaccaaa ctggcggacg cccataccgg agcattgatg acttccagtt ccccggtttt
    19441 caccttcctg ttggcctggc tgctgttgga agaaaccatc acccggcgca aacttctggc
    19501 attgctcctg tctatgttgg gaatcatcgc agtcattgga attaccggca atgagccgat
    19561 caatgtgggc ggcagtattg ctttaatggt agctgccatt acttgggcac tgctttccgt
    19621 atatgttaaa aaagcctcca cgcgctactc atcacttacc ataaccacat atgcgatttt
    19681 gttcgcgctg ctgtttacca ccccggctgc cattggagaa atgcagcatc agacggtccg
    19741 gctgtttgcc aaccctctgg tatggacagg aacattgtat atcgctttga tctctactgc
    19801 gctggccttc tttctctgga ataaagggat ggatctattg gaagccggag ccggatcgtt
    19861 gtttctttgt tttcagccgg ttgttggttc cttgttgggc tggctcttgc tcgatgaggt
    19921 attaaagaca agctttttta tcggggcggc ttttatcatt agcgctctgg tcatcgccag
    19981 ccttcctcca aaaaaagcct tgccctgact ggttacaggc aatcggataa agccatgcaa
    20041 gaaatgtctt tttcagccgt attataaaga aagattaaaa gaggtggagc attgatgaaa
    20101 aaaatctatg tttgtctgct gatgttgctg gttgtcttta ttcttacggc aggcgtccaa
    20161 gccaaactgg ttccggggga ttttattgat cttcatggtc attgggcaca gcaagacata
    20221 cagttactgg ctcaacttga aatcatgcag ggaacgggaa caacgccgca aggataccgg
    20281 gtatttgccc ctgacagcct ggtcacccgg gcgcaaatgg ccaaggtgct gaccgatacc
    20341 ttccatctcg attatggtca gctgcgtttt atcaagcagc ctgtagccag tgacttttac
    20401 cgggacgtgg acaataacag ctggtatgca gatgcggtat taatgtgtgc cattaatgaa
    20461 atattttata ccgattacaa tttttcgccc gcacacaacc tgacccgaat tgaagttgcc
    20521 aatgcgattt atcgcagctt tcaggcaaaa aaaatttccg tcccgatgat cctaatgatg
    20581 ccggtctaca acgataccca ggacctggtg caggatgata tgaatgccat ggtctttgta
    20641 agcaacaccg gaattatgaa aggcgacggc caaaatttca ggcctaatga ttcgcttacc
    20701 cgggcggaac tggcagccat caccagtcgc tgtatcgaac tgattgctct taatgaaaac
    20761 aaccacgagc aggaatacgt tatgaaatcc ggccaggaat ttattttatc cctggcatcc
    20821 aacccaagca ccggttacag ttgggatttt aacaagtcct ttgatgacaa actgctggaa
    20881 ttggctgttg acaagactta tagagcggat tctccagtca atgaaacaat ggtaggccaa
    20941 ggtggcagaa cctattggaa atttaaagcc ctgcagaatg gaaaaaccga attggaacta
    21001 cgttatgccc gtccctggga aagcgttcag ccgggccaga catacaagct gacgattcat
    21061 ataactgata ccgatccggt tactcctgag gacatttcca ttcagaccaa ggcatataat
    21121 cgtctggccg aatacatgga aaccaatctg cgcattcctt gcttgcaggg gatgcctgat
    21181 gaagtgctgc aagccaaact gaacgatcgc tttgtcgagg atgccttggc actggaaaaa
    21241 atactacagg cagatgtaca acaatacgtg gcaaatgcac aagcttccgg ttttcccatt
    21301 cacaattttg tgttgtattc gcgctttcag ccaggatatt taaaccagca cctgctcagc
    21361 ctgacaattg attattatca gtatactgga ggtgcgcatg gaataaccga gcgcaggccc
    21421 tataatgttg atttgcaaag cggtcaggat cttaccttga aaaacctctt cgttgacaac
    21481 tatgattacg ccagtgtcat taatcaggag attaaaaaac aaatcagcaa cggcgaacct
    21541 gtttatttcg agggtaacag gggatttcag gaaattagtg acaaccaggc tttttacttg
    21601 caggaggaag ccttggtgat ggttttccag caatatgaaa ttgctcccta tgcagccggt
    21661 attcctgaat tcaaaatccc tttgcgcctt tttggagata aattcagagt ggatttattg
    21721 gctgccaagt gatttttact gcggcccatc ttgattttat ggcaatcatc gttttttgct
    21781 aggttttttg tctctgcaat tatataataa tagtgacgtc aaacatggag gtgaagatca
    21841 agccatgaat ttaagagaag cgctggaact ggcagccaaa gaagaagcca aagccatgca
    21901 gaactatacc agactggccg gtgaggctga agatccggaa accaggctga tgcttgaaca
    21961 aatcgccaaa gaggaagccg gtcaccttaa acggctcacg gaaagactga aagctataaa
    22021 aatgttggga taggatattt aaaaacagct gtcggcgatg tcggcagctg ttttttcaat
    22081 gcccggttga gtgaccggct gccaggatgc tgccgaccac gataaaaaca aatagcagca
    22141 aaaacaaaaa caaggcaatg cgcagtttgg tgcgcacatg atgctcgccc tggttgtatt
    22201 ccgcaatcaa atgggttgcc cagaccacat tgccggaaaa caacataacg gttataaact
    22261 gaatccaact gggaatcacc gtataggcag catgttgcag atgtgaccag agcgtatgac
    22321 tgtaataggc caaaacgata aattcaatca agccggcggc atattgataa accagtcctt
    22381 gtttctgcat ttcggtattt acttccagca accctttaat ctgctcctgg gttgcaatat
    22441 tggtacggct gttcataacc tcaattttgc tttggaccac attcatggcc gcccgataat
    22501 cctcctgaac ctcattgagt tcatcccgta atctgttcat ttcatcccgg cgttgctgat
    22561 acggttcggt taattgggca aaaacagcag caggttcctg ccagaatgct tcgacattaa
    22621 tctgcctttg cagggcaagc atggctgcat cgaaattctt ttgtccgtca acaagcatat
    22681 tctgatcctc gaccagtttg ccataggcag cagccagcgt ttgaatttca ttttccaatt
    22741 cctctgccgc cataagagtc gcctggttca tgaccaactt ggtgtgcaga actttgatca
    22801 acccccgctg gatctcctct ttttcccgac caatggataa caggcggtca tgcaaaagaa
    22861 agtccaaggc ttgcaaacgc aatacgcccc catgtaacag cggcaaacga cgggaaagga
    22921 aggacaaatc atccgtctca ttattatccc gggagaataa ataaatagtt tgatgcttgc
    22981 tgcggtcacg cttcaggcat gacaatctgc ctttctgcag tgtgtaaatg tctccgccgg
    23041 tctgggcagc tgcgacggtt tttgcggctt cttcccgttg ttccgtacct gcgattatgg
    23101 ttaacacttc cactatgtcc atggccgcca aagggtcttc ttcttcccat gttcgcaatt
    23161 ctttcaaagc cgtctgccag tgctcattcc catgcgtcgg tttgaccgcc aatatcttca
    23221 gtttcccctg cgggtagtcg aggttgtaca tggtaccctc ggtttgccgg tctgattcga
    23281 aagtattgct ggtgtccagg agcagcccat gaccatgaaa aatatccgtc agcttttgca
    23341 gagattcgat atcctctgcc ataaaataat aaaaacgcca taccaattga gtcgtgacga
    23401 tcaactcctc tgcctgccgc tatgcacgac aattctacca ttatattcgg caaaggttat
    23461 catattcctt taccgtccgg caaaaactac ctgcaatttt ttggcacctg catccattat
    23521 tgataatatt tttatattgt ggtatcatat attaaactaa atgaaaacag aggcacttcg
    23581 ttctagcgaa gtgcctctat atatttttca ttaaattttc catttaatgg agaaggagtg
    23641 tatttccatg agtaaaaaaa taacgctttc aaaattcgta tttgatcgat tatcgaaaca
    23701 cttcaaggat tttactgaaa cacaaaacaa acttctgaac gacacggaaa tggttcccgc
    23761 ggctgagcgc aaaacctatc aagagatctt tatgcaatat ttacaacatc tggagcgttt
    23821 attaaaagaa gcacaaataa ctgattccga agataacagt cttccttgtg taaccttcgg
    23881 tagtgtggtt gaattggaaa acctggagaa taaacgtatc aataagattt tcgttacgta
    23941 cccgacggcc aatccgccgg ttgataaaaa gatcaaggta tcattgactt catataattc
    24001 ccctgtaggc aaagcattgt ttctgaaaaa acccggggac acggtggaag tcaacgcccc
    24061 cggaggcaaa ttcaaatatc gcatcaaatc agtggatctg atgatttcct gatcccttca
    24121 ggttaacaat ccagccattc cttaagctgt tcgctgatca agccagtcaa ttcaggcagc
    24181 gccctggcaa ccgggggact taattgcgtc ccccaatcca gacattgcgg ctgaattcca
    24241 agcaagacca aatgctgcgg caatctgccc cgcaaggcag ctaacgccaa tatctcctgg
    24301 aagccggtct gatgggtaga cagatgaccg gatacctgca tgggcatgtc ttgtactgca
    24361 agtttgcaaa cagtgcccgg ggcattgtct cccattactg catcaacgac taaaagataa
    24421 tcggttgctt ccactacgtt cagcaaagcc aagccatccg tccccccatc caccagctcc
    24481 actccatctg tgattaactc ctgcatgagc cgcttgatca aatggatgcc caaaccttca
    24541 tcccgaaaca aaggattgcc catccctaaa atcacgatct tattctttct catgactcgc
    24601 tccggcttcg tcaatcacat atttataacc actgaaaatc gaagatatgg tgccgtcatc
    24661 atccaaaatt tcctgacgaa tcaccaagta aagatgcgct gcgataaata caacaaaaag
    24721 ccaggcgacc aaatgatggt acatcctgac cggggaagac ataccgaata aaggaattac
    24781 ccaaccaaaa aagcgttcct ggaatccccc cggcgcaatt tctccctgca tggccagtcc
    24841 gctcaaaatc atgaacagcg aaccgatcca gagtacaaaa aaataagtga gctgggcaat
    24901 cacgttgtgc ccggtatgca ccgtatgctc ctttttaaga aacaaatagt acttcagact
    24961 gtccatccca tcggcaacaa atcctttgcg ccatggtttg aattgggcat gctcattgcc
    25021 ggcaaatgcc caatacaatc gaaaaagaaa atttgcgata aagaaccagg cggtcagtgc
    25081 atgccaaagg cgcatttttc ccatgataaa gttggcatat ggctctccgg tttgaaaggc
    25141 aaagggttta tcgatataaa gaccagtgat caacagcgcg ataatcagca ccacattgat
    25201 ccaatgatac aaccgtatgg gggcttccca gacataaacc gctcttttca acataagcct
    25261 tcaccccttt taacagaata gacattcgcc caaatcctgc cggctcttca gatcgataat
    25321 atgagccgca caggcgatac agggatcaaa ggaatgaatg atgcgcagga tttccagcgg
    25381 ttgttccgga tctgccaggg gcgttccgat caaggctgct tcatacgggc ccttgcctcc
    25441 cttgctttcc ggcgctgcgt tccaggtggt aggtaccacc gcttggtaaa gactgatttt
    25501 gccattatta attttcaccc agtgtcccag cgctccccgc ggtgcttcgg taaatcctac
    25561 cccctgggcg gaacttggcc aactggtggg ttcccatttg ctgccgtcaa acacccgact
    25621 gtctcccgcc tttatattgg ccagcaataa atcatagctt ttttgcaggt aatcaaccat
    25681 cagcttgctt tccagtcccc ttgccagggt acgccccaat gctgagaata atgcctcctg
    25741 accaaggccc agacgctgca acgcttcatc aacgctggat tttatggggt cattgccgcg
    25801 ggcatagccc accaacatcc tggccaatgg cccgacttcc accggtttgc ctttccagag
    25861 cggcgtcttg atccagctgt attttccctg ggtatccagt tgttcatagg gaggcttggg
    25921 accgctgtat ttaacgcggg tttccccgtt ccagggatgc ctgccttccg gcgcgctgct
    25981 gtattcatac caggcatgat ccacaaattc ttcaacctgt ttagggtccc ggggatcaac
    26041 gtcaaaaact tcgtttaaat tgcgatttag gatcactccg cctgggaaga tttcgtcgcg
    26101 gttgttgttt ttgttctgct ccgccaacgt tccgtaaacc agaaaatgat cgatcccgcc
    26161 gccataggtc cagtctttat aaaaagaagc gatggcaaac agatcgggca ggtaaacctg
    26221 ctcaacaaat gccttggcct tgctgatttc tttgaagacc agccccaatc tttccatatt
    26281 gatggtgttg tcattgtcaa tgttgatggt acaggccatc ccccccacca gatagttggg
    26341 atgcggattt ttaccgccaa aaatggtatg gattctggcg atatcctttt gccaatccag
    26401 cgcatcaagg taatgggaaa gagcgattaa attgacctcc ggcggcaatt tataggccgg
    26461 gtgcccccaa taggcattgg cgaaaatccc caactgcccg ctgtccacaa aacttttgaa
    26521 ggtcttctga acatcggcaa aatgccccgg tgaagagcgt gcataattgg ataacgattg
    26581 agctaacgcg gaagctgcct tgggatccgc tttcaaggcg gaaaccacgt caatccagtc
    26641 cagcgcatgc aaatgataga aatgaatcac atggtcctgc acaaattgcg cggctgtcat
    26701 gatctggcga attaaaacag catttttcgg caccgtaatg tccagtgcat cttcgactgc
    26761 ctgaatagag gcgatggcat gaatatgagt acatacgccg cagatacgct gtacaaacgc
    26821 ccaggcgtca cgaggatcac gatcctgcac gattctttcc aacccgcgaa aagccgttcc
    26881 ggaactaaag gcattgacta tttttccgtc ttcaagttct gcttctatac gcagatgccc
    26941 ctcaatacgg gtaatcggat caaccacaat acgttgtgcc attattcatc ccctcccttc
    27001 tcaactcttt tttgttgagg ttttttgcgc attaacactc cggttgccaa gccatgggcc
    27061 gctgcgcccg ctactgttgc tccggttact gccaggccga ttttgtccgg gttaaaaggc
    27121 atggctgttc ccggaatatg ggccatgcgg ctgtacatgg gcatatcatc ataaaatcca
    27181 ggttctgaac aaccgataca aggatgacct gatttaatcg gataacttac accattgttc
    27241 cactgggtga tggcacaggc attataagtg gtgggacctt tgcagcctac cttgtataga
    27301 caccagccct gcttggcacc ttcatcatcg aattgttcca caaacaagcc agcatcaaaa
    27361 tacgcccggc ggttgcaatt gtcatggata cggtgattaa aaaaggcttt aggacgaccc
    27421 agcgtggtca gttccggcag tcgatcaaag gtcaaataat gaacgatcgt cccggcaatc
    27481 acttcggcga ttggcggaca gcctggcaca tcaatcaccg gtttgtcttt aacgacttct
    27541 tttactgaca ccgcgccggt cggattggga tgcgcgccct gcacgcaagc gttggttgcg
    27601 catgatccaa aagcaatcac cgcaactgaa tcccgggcgg cctccaatag gatatcccgg
    27661 gctgttcgtc ccccaatcgt acagtaaaca ccgtcatctt gcaaaggtat gctgccttcc
    27721 accaccacca gatagtgatc cttgtaatcc tgcatggttt tcgccttggc ggcctctgcc
    27781 tggtgcccgg cggcagcctg caaagtctcc atgtaatcca gggagatcat attgaacaac
    27841 atatctgcca acaagggatg cgctgttctg ataaaagact ccgcgcaaca ggtgcactcc
    27901 tgcaaatgca aataaataac cggtaatctt ggtttatcct ccaaagcctg ggcaactttc
    27961 ggcacaaaag aagcttccag ccccatcatc gcggtgagaa tggcgcaaat cttcatgaaa
    28021 tcgcggcgtg aatagcctct tttgatcatt gcctcataca agctgtctct caccaagcca
    28081 acctccttgt attcaggttg cctatagtgt tttccattgt ttgcgaatat atgcaaaaac
    28141 ccggtagcgc tgggacaaga acgaggctgc tattaaatct cccttttcag aaataaaaaa
    28201 ggaccaaaca aaaacgatcg gtcttgcgat ggtttttgtt tgatccataa cgctgtcagg
    28261 caatgaaaaa ggagccagta aactgactcc tcaacatgca aattcagctt atatcctagc
    28321 ggcgtctagc ctggaagcaa tctctgcaat ataccggtct gtccccagtg ggctcaaacg
    28381 gtacagtcgt ttcttggcca caagccgcgc agacggtttt aaacatgaca cgctcttctc
    28441 tgcggccgga attaccaaag ccaccattgc gttcctgttt acgagccctt ctgcaagacg
    28501 gacaacgacc cggttcgttc tcgaatcctt tttcagcata aaactcttgc tcagatgctg
    28561 aaaacaaaaa ttcgctgcca caatctttac acgccaaata cttatcctca aacatgtttt
    28621 catctccctt ccggaagtac tcttggacct agtttccccc caaacaagga gatatttaaa
    28681 gaacaaggca attgcaattt taaatcatat ttggttcaat gtcaaatgag atttagcatt
    28741 aaatgaataa aaaaagggaa atacccttaa atgatatcca ggctgacttt ttcgggatgg
    28801 cgcggtactt tgtctttgta aaaatctctg atctgatgca tatcctcaac gagattcccg
    28861 gtaggataga tagccggtcc aaaaccgcca gtcttctttt cgtaatccag ataacttaaa
    28921 acgatgggaa cttttgctcc cagggccaca taataaaagc ctgttttcca tttcctggcc
    28981 agtttgcggg ttccctccgg aggaatcacc attacgattt catcccgttg attcatgatg
    29041 tcaacgatgg cagcaattaa attattgctt ttgcttcgat ctaccgcaat cgcaccggta
    29101 tatttaaaaa accaacccag cgggaatttc atccattcct ttttgattag atacttgatc
    29161 ttgacattca ttattctgag aatcgctatg gcaatgggaa aatcataatt actggtatgc
    29221 ggtaccgcca acagcacaca tttttttatt tcgggaggta ttgcgcccgc gatcttccat
    29281 ccggccaatt tgatatatat tttagatatg aaccttaaca accgttatcc tgcccccgtt
    29341 tcttgcttat ttttacaaaa caaattcaca cgcgcctttt cagtgaacgt ttctattatt
    29401 gctgttttcc cctgtcatta caagttcata tcaggcatga attttatcca cccagcgacg
    29461 caatcgtgtt tttttaataa taatcgtact ttccgggcac aactcctggc agcaaaagca
    29521 cctgatacag cgatcataat catagacggg cggcctttcc tgatcgcctt gcggccaggc
    29581 aagcaccttg ggaataaccg gacacacttt gatacacacc ccacatttga cacaacctga
    29641 ctcgattatg caaggtttgg aaaggagaat ttctctgagg agctttgatt gcggaattct
    29701 cctttggtta tggttacttc cccggttaat gtcaaacgtg ctgtccgcaa aggcttccaa
    29761 gacatctccc agaaggttga tgtcctgctc caaacaagtg cccaagccgg acttcattcc
    29821 tgcttgtatt gtagaaacag tctccggaat aacgccaacc atgcgacaga caaccgcatc
    29881 cagcgcaacc ggatcactgg aaaagagcag tacgttcatt ttcttgggcc ttccgccgcg
    29941 gggcccgttg ccctccatgg caatgattcc atccataata aataatcttg gctgcaatag
    30001 gacattaaga tcgaccagca tctgggcaaa atgactgacc tccggcagtt tcacatgata
    30061 ctcacccttt agaaagcctg gaatacagcc gaattgattc ttaatacatc cggtcatgcg
    30121 ggtcagtcca tgggttttta atttgggcag actgatcaag ccctcgctgg ccagaacccc
    30181 ttgggcaatg gtaaattttt tattctgcag tgcttgcgcg catacggctt cctgcccctg
    30241 atgaaaatct gccaagggaa tccccaattc tgcagcgacc gccgcaatgc ccgatttgcg
    30301 ggctacccct tcaggactgc caaagccggg agagtctcca tagcttaatt gtacaccagc
    30361 cttttgcagg gccaaagccg ctgccttgaa tacggcagga tgtgtagtaa cacatttctc
    30421 aggcggatca gccgaaagca tattgggctt caacagtatt ttttccccgg gtttgacgaa
    30481 gctgcgcaag ccgccgatga gttcaagtcc gcggtgaacg gcagcatcga cttctcgctg
    30541 ttcatagctc tcacatctta cgatggcaac ttcagccata tgacttcctc gattctttta
    30601 tttaagcgag cccatgaaaa atgccaccat gctgatcagc gcgatgccaa agccaatgat
    30661 ccccaggaag gcaaatcgct ctaccagcgc attgtgattg gcctgaatga tcatggccga
    30721 gccaatcaag gttgctgata tgatcaagct gaaactgagc cggttgacca tctggccagt
    30781 cttgtggtat agcttggaag acagattgac gtcaaggttt aatctcagct tctcatcgac
    30841 cgtgtcctgt aataaacggt tgaaatttcc aggtattttt ttgagcggtt caatttcctt
    30901 gtagtagcgg ctgcgtaaaa acttgcccaa gttggacggc tttaatcttt cccggtaaac
    30961 cttctgggcc aaaggggaag ccacttcccg aaagtcaaac tgcggatcga gttttttgcc
    31021 cagaccctca accgtaatca gtgccttcat caaagaggtc aggtaagaag gcattttcaa
    31081 gtggaaacga taagcaaatt ccatgatttc actacgcatc cgtttcatat ccagactgcc
    31141 aatactgctc gtatacagac tttccaccag ttctgcaaaa gcctcggtaa agtcttcttc
    31201 atcgtccagc tgctcgagaa tgcccatatc acgcatgaca ggcatcgcac gatatgggtc
    31261 gtttttagca atcgcgatca gcatctctcc tacataggcc agtcttacct cacttaaata
    31321 accaatttct ccaaaatcga taaaagcaat atgttcgttg tccagcacga aaatattgcc
    31381 cgggtgcgga tctgcctgaa aaaaaccatg caacaggatt tgtgataaga atgcctcggt
    31441 tcccaaagcc gatatttttc ttctatccag gcctttttca tcaatttttt caatatcact
    31501 tagctttata ccttcaacat actcctgcac caatagccgc ggagtactgt attccttaaa
    31561 tacacgcgga atgatgacgc gctcatcgtc cgcaaaattc ttgtagaccc gctcggtatt
    31621 gcgggcttcg cgctcataat caagttcccg cagcaaggta cggcggtagt cctccaccat
    31681 ggccaaaata cccagacgac ttgcttcaga tgaacgtctc tcggaaatcc gtgccaatcc
    31741 cttgagaatc tcgaaatcat ttctaacctt ggcttcaatc tcaggacgct gtaccttgac
    31801 aatgacttcc tgtccgttgt gcaaccgggc ccggtgaacc tggccgatgg aagctgccgc
    31861 cagcggctgc gtgttaaaat cggcaaaaac ggtctcaact gctccgattt cccgatcaag
    31921 aacttgcttc aaatcctccc aactgatagg atcaactttg tcctgcagct tttccaactc
    31981 cccaatgaaa accggcggaa gaaggtctgt cctggtgctg agcaattgtc ccaacttgac
    32041 aaatgtaggt cccaattcaa ccaaagcctc gcgcaaattc accgccaata aatactggtt
    32101 tttaaccgaa cccggaacgc tggtgcgcgt gcttttcaaa gctatgcgtt cactaaaacc
    32161 aaatctttgc aaaaaatatc caagtccgtg gcgcataaac gtcccgacaa tttcccgccg
    32221 gcgcttgata tatttcaact gccatctgga attcaccgcc agaccccctc cacgattatc
    32281 tgctataagt attccagtat aaaagtcaga ttccttttaa aaaagagatt ggtggtgtaa
    32341 tttataactc tgaacatgct gcctctctgc aaaatggctc gtatcattta atgaaagtga
    32401 tacgggccgt aattacaggg gctttccgcc atcatttatc agtttattat ttgcgttctg
    32461 cttcctctgc cttcagcact ttacgcatga ttttacctgt gccgccttta ggcagcgttt
    32521 cgcggaactc gatgagccgc ggatatttat aggctgacat ttccttgctg gcccatgcga
    32581 ttatttcttc ctcgcttact ttaccgacat aatccggtcg caggacaaca aaggctttga
    32641 cttcctcgcc tttgtattcg tgcggaaccc ctatcacaca gcattccaag atggccggat
    32701 gatgatatag atattcctct acttcagccg gatagacact gaacccggat accttgatca
    32761 tttccttggt tctgccgcac aaccaaagga aaccgtcatc atccatctta acaatgtctc
    32821 ctgtcttaag ccatggaaac tcgctgtcgt ccgattgcac aaatatctca gcagtcccct
    32881 cggggttgtt ccagtaaccg atcgcaatgg aagggcctct ttgccacagt tcgccttcct
    32941 ctccgatctc cgcgtcaatg ttttcatctt tcaggctggc gatccgaaca tcaaccaagg
    33001 gacagggaat cccaatggag cctactttgg aataaccact ggagttacat atggtatatg
    33061 ccatagtctc gctcatccca aaaccatcct gcaattcaaa gccaaaaatc tccttgtatt
    33121 gattaaacaa agcttgcgga agaggcatgc ctccagtgcc agcgaatgta agtgaagaca
    33181 gatcgtagtc cttgaatttg ggatgttgga gaaatgcaat attcatcgtg gtaatcccga
    33241 tccaaaccga aatacggtat ttttcaatcg cctgatagag tgtttccaga tcaaaacgaa
    33301 ccagcaatac aatcgtcgct cccattaaaa caggtgcgtt cagggaatta ttggatccgt
    33361 tgacatgaaa gagcggtaat acggcgacat gaatgtcatc gaaataggtc ggtctgccat
    33421 aggcagaatt ggtggcattg gccaacacat tgaaatgcgt caggattgcg cctttagggt
    33481 taccagtggt gcccgaagtg tactgcagca tggcaatatc ctttttaatg tccagatctg
    33541 ccaattcttg gatcggttca ctctccacca gttctttcca ggtaatcgta tttggtaatt
    33601 ctcctgcctt gcctccagga tcaaaactca ccaaaatctc aggtttttcc ggcagatact
    33661 ctcccaaaga agtgaccacc actttttcga cagaaggtac ctcatctctg attgctgcaa
    33721 aaaccggaaa aagatcatct tcaaacaaaa cgactttggg ggtacagtcg tttaagacaa
    33781 aacgtaattc gcctggctta tacaaagggc ttccggtgat aaccgtcgcc ccgatggctg
    33841 acgctgcata ataggcaatt ataaactggg gacaattctg cagggcaata tataaccggt
    33901 cacccttttt catccccatt ttctgcaatc ctccggcaac tcgccagacc aaatcattta
    33961 attccttgaa ggtgataacc cgaccataaa aaatgattgc tggtttatcc gggactctcc
    34021 aggaatgatc ataaagatgc tttaccagcg ggtcttttcc atagtggaaa cgatccggcc
    34081 accaatacgg tacatttttt tcccaaatct ttttgtccat tgctttatac aatgactact
    34141 cctccttcat gatattcatc gagccggcaa ctctaatctt tcctcgcctc ctcaagcatt
    34201 tgcctggcta tttgaccaac aaccttccgt tacaactaat acaactaaaa cccagcggta
    34261 acagacgcat aactaccgca aatcttttca tgcaaagtgc ctatcacgag aggctataaa
    34321 tatcacttca atatttagac aaaatatcct cttattatta attattgcca tatttattcg
    34381 cgtatttaat tggctaaatc tggaattgag ctaaaacctc ggttttatcg cggtaactca
    34441 agcttatatt gctttatcac atgacaccct cagaaattgt tataatataa caagacagtt
    34501 caaagcggga ggaaagcaaa tgcaggacac aaatgaaaaa aatatttttt tgaaaattgt
    34561 gcgggcgtcg gcgacgaata tcgtagggtt tgggttggca actgcgctgg tcagctattt
    34621 ggtgagaaat actgatgtaa aaacaccgct catggttttg gcgggcttga ttgtttttat
    34681 catgttggta agcctgagct ctctgatgat atttatctat tttacgataa aaaatatccc
    34741 ggaaaccatg cgtaaggctg gcggtaaaag ctttggggaa gtgtataaat atattttatc
    34801 tgcgctggcg gtgcgtctgg tggaagcggc catatgcatt gtctttatcg tccatatata
    34861 tcatctttat ttttgattta tcgtataatc tgttgcttac gcaagctttg ttcacatcaa
    34921 cttaggaata tatgacgatt cattgaagac tggcttgatt acattttgtt gatgagtatc
    34981 aacccgacaa tacaaaaaca tgcaccaatt actttatata cattgaagct ttcatgataa
    35041 aacaggatcc ctacaggaat cagcatcagt gccaggagga tattggctac cagcgaaccg
    35101 acactaatgt tccatcccgc tctgtaagcc agcaaaaaac ccaattcaag tcctaatata
    35161 gcaaacccca ggacaatact ggtccaatta agcgctgcga aagattgcat aaaccctttc
    35221 tcggagcgcg tgaataaaaa agcaaccagg gtaaccgaaa tcgctgtcag ataagtgacc
    35281 agtaacgcag ccatcggatt ggcattctgg ggagttgact tttgggcaat attataaaat
    35341 acattggata tgacaatcaa catgatggat aatacataca tttacacgaa cacctcacaa
    35401 aaaaacaacc atgatgttat ccatggttgt tttcaataat acaggtttca ttatacaata
    35461 acaaattatt gatgatatat atgatacccg tttattttat taattcgtaa tgcatggaat
    35521 cgctgtagcg gttgccccag ctgaacccgg ccggtttgaa ggctttttcc cacagaatca
    35581 aattggggtc gtcctgatcc tgttggggat caacatagcc aaaatgtccg ctggcattga
    35641 tatcaatggc ggttccccag gaatggttgc ttaatcctct ggaagaatcc cagttgacat
    35701 gacgagcaac atacgtgcca tccatggttt taatcaagct tagcaacgct acctcctgcc
    35761 cattgacaac ggccgtgcca ttctttatat agttgaaagc agtgataaaa ttttccgcgg
    35821 catttttatg tacctgcacc tgacgattca aaccgggcag cgttatggtt actatgtttg
    35881 tggccaccca ctgaggatca acttcaattc ttcctcctgc cagctcccga taacgaaatt
    35941 gcccaaagga accatttatt ttactgagca gcggatattt gctgttatcg attgctgact
    36001 ccccgcgcga tacttgattg ttgctggaat tggtgctgtt accctcgctt gccgggttgc
    36061 tgccggattc gacagggttg cttgccgctt tgctttcatg ctggttcacg ctggtcgtcc
    36121 cggacgatgg attgccgctt ttatcctgac tgctggtttc tgttggcact cgagctattt
    36181 ccggctctgc cggcggggca ggagactgaa ctttttctgt atttgtctga ttatcagcag
    36241 gcttcactgt aagtacctta tcaactgaaa catgcttctc caatctgttt tgagccgttt
    36301 taatctcatt ggggttctcg ctcccggtga gctggtcaag cggttgtatg atacccggta
    36361 tgtaaatgca tacgccgaaa aacagtaaaa atgccaaaat gcttgctgcg atcttatgtt
    36421 tcatattttt ctccctgtat gatagtttgt atcaaatgaa caacctgatt gccatataga
    36481 gcacttaaat atcatcctcc attttccaga tttaaactat gctcccacga ttgtttgata
    36541 attcatttgg atatctatca ttttataggc atcg
//
LOCUS       DAOTHZ010000013        35231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1337_length_35231_cov_8.010376, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000013 DAOTHZ010000000
VERSION     DAOTHZ010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 35231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35231
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1337_length_35231_cov_8.010376"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..3032)
                     /locus_tag="P5549_02920"
     CDS             complement(78..3032)
                     /locus_tag="P5549_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW12056.1"
                     /translation="MLKKHNKLIMLLVLVTFMFTMVGSAGAATFSDVTGTSVESAAIY
                     KLNGLGIIDGYPDGTFGAEKTITRAEFAKIACVTAGLKSVASGMGGTASPFSDVATDH
                     WANGWINVAAAQGYVKGDPAGTFRPEDQITQAEVVTVLLRLLGYNDNLAGEWPSDYVA
                     KAANLGILDDVTFVAGQASTRGIVAVIAGATLDENVVEYAASDNLFNESLKTYDKGDG
                     KGKISQSYTLLYDKFSESQKTEDALIMSFSVNDDDEYTIYYYTYDDNGNLSDANANTD
                     GYQPYSKKLADECTLPGGSILGAIENFADFVTNDDDEITSIVLKDYKKFGPKTAAGTP
                     LNEIVIDGGKVKIDGVKYSFADTAIVTESCNTEFAPIINAPLTALVKSIATTIGTKDS
                     TTAQWQAVQDAMLLNTIDASKIADKTYDASDAYAVIMNDDDEVVALRAWTFPTPGIVK
                     KVNGEKVTYQTGATPAAAGNPLQSVVTAQLIAAGVPAATAVTEVALELPNIDFKVQSN
                     SGSWDGFVNTTNFTDKDVYVQKDGKPATLADLQENDTLSVLENYRGCDYYLVATSTKI
                     NGTLASAQYDNNNVKSVKVMSMTWKAPWSNLLKTTYQGRYTDDANDPDYATLTQNVLY
                     NEYDAWNTDVSLLLSATGKVAGLILGDTESSKMYGVVTDWSLDMTAANGDTVRNVTIM
                     KSDGEEYSYGLAEDSYLQNAATGTRTKVEDLTSGGRAADYAAAEALLIPGTSAGAFTD
                     GILVSISLDSDNIIKTIKYWGGYAATTVANANMDTDEDLFKAADGIWYEAKDVVVFNL
                     VGTDLADTEVMGWKELKESAGAKYYSYYTNSSNELEYIVIPTTSAASLATDTKYAIYY
                     EQYSDSDDWIKFVGMDALEVDDIVSGLTKGDVVSYKLSGGKATNITKKIAYGTTSPLT
                     LDTLNKGTIVLSNGNSYKIDSEDTVYLDNSDDKDLQVLEGVAEGDNVIVIDDNNVVNG
                     NGDTAMAIIVVD"
     gene            3408..3773
                     /locus_tag="P5549_02925"
     CDS             3408..3773
                     /locus_tag="P5549_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12057.1"
                     /translation="MNKLQKYLCAAILVLVLAGIGWQYVGWQKQVERHENALQFWQVE
                     VPKIQAEFPEFKPQNAEQMMAAENKQFAEQKKTPYVNSGIIVLLGLMAGGAVIYGPMM
                     VAKIKLALQPQEASKKRKR"
     gene            complement(4083..6533)
                     /locus_tag="P5549_02930"
     CDS             complement(4083..6533)
                     /locus_tag="P5549_02930"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRW12058.1"
                     /translation="MLPVLLLTLLLISMTPAKAGAASGIVTGSVVNVRSSPGSNGDII
                     GQVLKDTEVEITALSGDWYQIKYGSLKGYIARELIAARQQVQVKNGPINARSGPGTDY
                     DIVASLGDKLVFAVLGEQNGWYRIQLPDGSLAFVAGWLVTLVDITATTTETVTTVPAA
                     TNPSPVSSAPANPSVILNGSQLQFEVEPRIENGRTLVPLRAIFEAMGANVEWNDSTRT
                     VTARTSNTTVVLPIGSTSPTVNGQVWPLDVPAKIVNDRTLAPLRFVGEAFGGKVDWNE
                     QTRVINISHTAGALPPAVLVTGGSVNLRDNPSTEAAIVEVAQAGEKMTVLAEKNSWYQ
                     VSRGGRNTWVAAWVVDPLTDASVGGTTEPGNGAAPSEPAAGNDQEEPVVIDEADINRH
                     GDENVAVAEGYITISTRLASDGYRLVMKTGEKAEPIITESRDGKKITYKFDQAVLKST
                     RDEAYFYIGSGKNAKVTISAQSMEDATWVTVEMPLAYQYTNTTEANGCKQVFTITSQL
                     TTVEDITLKNGNQIIVLRATSAMEYQESFSDGVITLNLSKTGQGFVEDDYLFSGLAGR
                     VSLSQDKGKPLQIKVNTGKNLETYQVNRSVNGLKLSIILVNKDKKVMEEEPTKPTDDT
                     DNQVTIVLDPGHGGRDTGSISKSRTLYEKDVVLSMGLKARDILEKAGYKVIMTRDDDT
                     FVGLSDRPAMANKAKADLFVSIHCDSVDSTEPHGTGTFYYAPESTPALYAQEYQRSLL
                     ADLLQKAMLEKCGRTDRSIRQANYAVLRESNMPSALVETAFISNAEEAQLLASADFQT
                     KAAQAIAEGIQKYVKLML"
     gene            complement(6905..7972)
                     /locus_tag="P5549_02935"
     CDS             complement(6905..7972)
                     /locus_tag="P5549_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRW12059.1"
                     /translation="MSKLKLLLKLVLVFSLVFMLSFGLGASLHNLLLADDDPTEEQEP
                     TVIDGERTNILLLGTDARPGETHTRTDTIILASIDPELNKMALVSIPRDTRVTIKGST
                     DKINSANAVGGPELVADKVEEIVGEKIDYYVEMDFAGFKKIIDTIGGVDINVDQRMYK
                     PSEDIDLQPGQQRLKGSQALAFVRFRDYALGDIERTEHQQIFLKALGDELLQAKNIVK
                     LPALVKVVKQNIDTNLGMTDMLKMASWAPGFSSDSIIAQTLPGYFWDQRDEYGNLTMS
                     YWVADQDKTEDLLEKMLAGQTVAVVSDSPVAYNPGNQSSVKDKNKDKDDSESEAEQTI
                     EELNWERSRLPSAGHGVKNKV"
     gene            complement(7998..9062)
                     /locus_tag="P5549_02940"
     CDS             complement(7998..9062)
                     /locus_tag="P5549_02940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRW12060.1"
                     /translation="MSFLTSALILVSAFVIAYFSTPLVIRLAHRLGAIDQPDKRKVHQ
                     TAMPRMGGLAIFFAFMICVLLGVKVSGPYVGIVIGAVIIFIIGILDDVYQLSAWIKLA
                     GQIIATAVAVYFGVVIHFVSNPFDGMLALGYLSIPLTFLWIVGITNAINLIDGLDGLA
                     AGVAGIAGMTMGIVALMQGQMETAIIAFALVAAIMGFLPYNFHPARTFMGDCGSNFLG
                     FVLGCLAVTGVAKSTALISLVIPIVILGIPIFDTFFAIFRRIYKKTPIFMPDKDHLHH
                     RLLKLGYSHQTTVLIIYAISGFFALTAIVLSWFDNPKATLVLAFLLLFMVIGANRIGM
                     LTGQTKASVHSTDQSTGISS"
     gene            9274..10356
                     /gene="csaB"
                     /locus_tag="P5549_02945"
     CDS             9274..10356
                     /gene="csaB"
                     /locus_tag="P5549_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HRW12061.1"
                     /translation="MKIALSGYYGFDNAGDEALLLAISSSIKSLVPDGEFVVFSGNPI
                     KTRQLHNIGAVYNMNPWSVARELLSCDLLISGGGSIFQDVTSSRSLLYYISVVVLARL
                     LGKPVVFYAQGVGPIRKPFNRWLLGRVANHVNLITLRDQASREFLRKLGVSKPPLQVT
                     ADPVFALQTNEDIKADIKHRYPLLSSPATKRLGVSVRHWPALQGYQQKLAVALDHFKR
                     AGYEIIFVPMDYPEDIKESERVSACMQEEAWIITDELTTAQHLAVIAQLDLMVAMRLH
                     ALIFAASCGVPFAGISYDPKIDAFLDLFDKRPLSPSSELMIAQIEELLYDQNAQDQVR
                     HRADKMRAQSLENARMTLQVLKNSRR"
     gene            10474..11985
                     /gene="murJ"
                     /locus_tag="P5549_02950"
     CDS             10474..11985
                     /gene="murJ"
                     /locus_tag="P5549_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRW12062.1"
                     /translation="MEQENTRTGKTFIGVSLMIFISKVFGFARDIVFASVFGTTVLTD
                     LFQVIFSLPSLLFSSIGNALSFVNIPDLTYYLKHGTREERNTYLSNLYAQVALGAALL
                     SLLGIIFAPALTKLIAPGLQEEVTSMALLLTRIMIPTLVFVTLTYITAGVLQVHGYFL
                     LSAAISIPFNFLIIASLLLKGQDLIFLGIVTTLGWFLQFLIQVPVLFKEKYQFSLKID
                     WHNQHTVNMFKQLLPILMGNSLLQLCLIIDRSFGTHLDEGTAAALGFGSNLFMTITGV
                     FIIALSTVIFPRLSQYCMDSDYRQVRSMLRYGFKILAFILLPYLILVTAYSPDIIALV
                     YERGAFTSNSTHLTATAFLCYSFAVVGYACQEVFNRVFYALKRFKTPMYASLVCLTLH
                     FVLNLLFYRQAGIAGLALSTAVCLLVYALIMGLIVRRVIGPFLGQNFANYSLRLCLPA
                     AGMIATILVCRYLFVSGLLLSFLLPLLASGMVYLLVAWLGGFYQELINRGDPV"
     gene            11985..14015
                     /locus_tag="P5549_02955"
     CDS             11985..14015
                     /locus_tag="P5549_02955"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HRW12063.1"
                     /translation="MNNREHAFLWLALAVALLFSITGFWLRISNESMNNTLVTVADYQ
                     LFEKGAQSSSLSMDEVLADLKASGVKTIAVKETTLRGLAARGDAYVASFADFNNTVRT
                     YQPDVWAIVKQLPDMEEISPANLVVSVESPETASFLQEKLGNRFTPDQIVNFPAGGRQ
                     YFIIKAELEALDKSKTNIREYDVQLGFETSILQKLKAMGFAIVLRPGDSNGSNRDYLQ
                     EYEPLVRAYDVKTLIFNYNRVSGSPDHLDVMARLIDKYDLTIGVIETSKQLGIIEQDG
                     LNEVMSATKYPINRVYSTGNDDFVTSVDERYYRWIRGVVDRGIRILYVVPFNDDKKIA
                     SVNVSETIDTVARFLPAITAKGFVVDQTVLPYGLNSETTTAAHRLAISLSLLAALLLY
                     LLYLWHPQRHWMRAIAILGLLACLAINIVLHMDFSKIYALAAAVLYPAFSSLLLLIYL
                     KKYPRHGLMKKLWRSLAIILVINALGMYTIVSSLADIRFIMNLSYFSGVKVAFITPLL
                     LFPLNYFCVTVPRENWLPWLRARLKESPSYLALALFMAALVVVYVYIGRSGNDSGIQV
                     SSLEIRLREILENIFLARPRFKEFVIGYPALMAMVYLYHRYKNDLILLLLGFGVVIGS
                     ISMVNSFCHVFTAVSISASRTLAGLATGLLLGTMLVIAIWLAEKIWAKYGFD"
     gene            14199..15245
                     /locus_tag="P5549_02960"
     CDS             14199..15245
                     /locus_tag="P5549_02960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRW12064.1"
                     /translation="MDKQHLQEFDFLRALATLTVIGIHVSAAYVYTSMWGCLANQVAR
                     FAVPLFLIMSGFLLLHADLNKQTFSAPDFYRRRFSKILWPYFLWSLFYACLNGILGAS
                     LLAALLQLPIQLLWGTAFYHLYFVVIIIQLYLLYPLLYRWMIRAPRSCLLTGLALTLL
                     CQIVLNRYSLGQLAIPSQLNNLSLVFFPVWIFFFVFGMFAALCKERLVKLFKNRRGLL
                     ALLWILSLALVVIDGYLHNTYGSSSRPTVTLYTLISFFFFYALALHLRGAFRPVIAWL
                     ARQSFLIYLLHPLVLTGLITFAAWIGRADLWNRTRGMIAIYGLVTLLSCAAVYLVSLT
                     PLAEKLGGVRRTKS"
     gene            15275..16873
                     /locus_tag="P5549_02965"
     CDS             15275..16873
                     /locus_tag="P5549_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HRW12065.1"
                     /translation="MKKVFYVLAGMVFLIVLAAAGFYWARYYDHYFDEKKIVSADETN
                     TIVRVDKEYRRLYNHAGGYAINYPAQFRLDVREPSLKSDFSDDQHNIEVYSDNFTGKI
                     SDAKAYIGYSNGFLARDKDIQVEFNKYSVINGRLANLTKWQRAKLARVENDKNHYFCI
                     KLVENSHQVYTIVIKSSQPIDDREFMKLVKSFRPIEKNNDLAGIVYQPQPKNWNSETT
                     TVYDQYFGHQADLKWGIYEPTAPAKMKNLTWLEDRLDYDFHFLIRYQNLDKPLPVQEL
                     QSAWADQKLIELTLQLGFEFAEAAAPYNPKGIPLAQFNEKIIPELLNGTFDAYLAQYA
                     QDIKDFGHPLLFRLNNEMNGDWCVYCSYHYGNDADLYIEAWKYIYKIFADHQVDNVIW
                     VWNPHDLSFPDFAWNHYLNYFPGSEYVDIIGLTGYNTGTYYEGERWREFAAIYDPLYA
                     EYEKRFALPFMITEFGSNSVGGDKARWLQDMFAHIDRYPSIKAAIFFSSTDLDSNQQP
                     ARIYRIDETEAVLDTVKQGLARYQ"
     gene            complement(17041..18522)
                     /gene="lysS"
                     /locus_tag="P5549_02970"
     CDS             complement(17041..18522)
                     /gene="lysS"
                     /locus_tag="P5549_02970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRW12066.1"
                     /translation="MSEQNQQEIDLHELKRVRLEKLQELRDMGIDPFGSRFERDTNCQ
                     TIIDDFAALEGQKVTVAGRIMSKRRHGKAGFSNLQDLSGNIQLYFKKDDLGEERYELF
                     KKLDIGDILGIKGYVFRTQKGEITVHVEDMTYLSKSLNPLPEKWHGLRDVEMRYRQRY
                     VDLIVNPDAREVFIKRSRIVKEMRNYLDARGFLEVETPMMQPIAGGAAARPFITHHNT
                     LDMNLYLRIAPELYLKRLIVGGLEKVYEINRSFRNEGIDTRHNPEFTMLEIYQAYADY
                     QVMMELTENMVAEVLQQVNGSMQVEFEGVTLDFTPPWNRITMLDAIKEYAGVDFTVIS
                     GDDQARTAAARLGLKVDHRSTRGEIINEVFEEYVEEKLIQPTFVYGHPVEISPLAKRN
                     AQNPEFTDRFEVFVMQREIANAFSELNDPIDQKNRFLKQVEKRAGGDAEAHMMDEDYI
                     NALEYGMPPAGGLGIGIDRLVMLMTDSSSIRDVILFPTLRARE"
     gene            complement(18611..19096)
                     /gene="greA"
                     /locus_tag="P5549_02975"
     CDS             complement(18611..19096)
                     /gene="greA"
                     /locus_tag="P5549_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRW12067.1"
                     /translation="MAEREDREIILTKEGLEKLENELETLKSVRREEVAERIKQAIAF
                     GDISENSEYEDAKNEQAFIEGRILTLEKTLKNARLMTADEIRDDVVSLGTKVTLKEMK
                     SGREVNVTLVASVESKLKDGKISDESPVGKAIMGKAVGAKVEVEAPAGIIKYKIIKVK
                     R"
     gene            complement(19220..20191)
                     /locus_tag="P5549_02980"
     CDS             complement(19220..20191)
                     /locus_tag="P5549_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12068.1"
                     /translation="MPEFDFAILHMLSRNRRGLTAKKTFLDRLAPVLFSPRVEHLIHF
                     GDLGFKGCNVFLPLGPENWQQLESAQRGCMWKKSEGIMQDFAVTSLAADHRLRFALQP
                     GEAQFPLWFGDHFVKALAAVMVKYILEKRDVKRLILVGDMAALLPLLDHLGSCGLPIA
                     VQNQHPSGDEAIAWRLLYERGWAISNSYICPEKWQKGDLIVLFQPGFQRLAVTSPEVF
                     WLELTDEGRHLAPSLENALERAGMEAGLSILAPILESILYTKAGISYGNVELQNLHFA
                     PVIKTGADLTVLIEAGEKLGLWEPFRQGYGGGFLDNAIWGHYNTIKD"
     gene            complement(20184..21098)
                     /locus_tag="P5549_02985"
     CDS             complement(20184..21098)
                     /locus_tag="P5549_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HRW12069.1"
                     /translation="MKHVVSVSLGSSKRDHAFETDFMGEKFRIERIGTDGDWDKALQL
                     IRDLDGKIDAFGMGGIDLYIQIAGKRYVIKDAKKLVAAARKTPMVDGSGLKNTLERKC
                     IIDVQKEGIYDLHGKKVLMVCGVDRFGMAEAFEEVGADLTLGDFVYTLGIPFPIHSLK
                     TLRIFGRMAAPIVVNMPFDKLYPTGENQDVIIPKHSKYYYNAEVLAGDFLYIKRYLPE
                     KLNGQLIITNTTTHEDMVMLKQRGISKVITTTPDMGGRSFGTNVIEALMVTLLNKPVD
                     QISPQDYYAMLKELDFKPGVVDLETLRA"
     gene            complement(21118..21648)
                     /locus_tag="P5549_02990"
     CDS             complement(21118..21648)
                     /locus_tag="P5549_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRW12070.1"
                     /translation="MEFIRIQDKIISWQKIETSLKKVLQMRARGFSQQEVAERLKIDR
                     TFISRLETLGELRKGQSIACIGFPLSNKEEILAILEKEGVEYIMLMTEQERQDFINHR
                     SGKELLNVIMDLIADVRQYDVVVCIGSDERIRLMEGVLNGQIISIAIGTSPITEDKWL
                     DPAIIKEVLRSIRAAR"
     gene            complement(21805..22764)
                     /gene="dusB"
                     /locus_tag="P5549_02995"
     CDS             complement(21805..22764)
                     /gene="dusB"
                     /locus_tag="P5549_02995"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRW12071.1"
                     /translation="MLKIGSVKLENRVVAAPLAGITDRAFRTILQSMGCGLVFTEMVS
                     DMGLLYNSNKSRQIAEPGEEEGPVAVQIFGSDPGAMAQAAHMVEKMGADIIDINIGCP
                     TPKIVRNGEGAALMLEPELSRKIIRSVVTAVQVPVTVKMRKGWDDERCNYLQMADIAV
                     SEGVRAITLHPRTRMQFFSGQADWQAIAEVKRHSTVPVIGSGDINKAEDAVRMVAETG
                     CDAVMIGRGALGNPFLIRETIALLEHGQKIQAPSQKEKITLAIRHLELAIHYKGEHIA
                     VREMRKHISWYTKGMPAAARLRDQINHTVTRQEMMKILEGLLV"
     gene            complement(22774..23541)
                     /locus_tag="P5549_03000"
     CDS             complement(22774..23541)
                     /locus_tag="P5549_03000"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRW12072.1"
                     /translation="MILVFDVGNTNMVVGVFDQEKMVDHWRIRTDTQRTADEYGIIIH
                     GLFLYQNLQAEKIKAAIISSVVPSLNMELEWMCQRYFGCKPLLVGPGTKTGLAIKYDN
                     PREVGADRVVNAVAAYHKYKGPLIIVDFGTATTFCVVSQAGEYLGGAIAPGIKISTDA
                     LVSRASKLPRVELSIPKSMIGKNTVMSMQVGIMYGFVGQVEGIINRMKKEIGGDVRVI
                     ATGGLAALIAGETDTIDEVDEFLTLDGLRLLYELNHG"
     gene            complement(23598..24434)
                     /gene="nadC"
                     /locus_tag="P5549_03005"
     CDS             complement(23598..24434)
                     /gene="nadC"
                     /locus_tag="P5549_03005"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRW12073.1"
                     /translation="MDYPGLNELISRSLAEDMGYGDITTMSVVPQEQSSEGFLIAKAD
                     GIIAGLGVARAVFAQLDPEADFTCFLEDGCPVSAGSIIAKVRGRSRALLSAERTALNF
                     LQRLSGIATQTHYLTQLIKDYPAQLLDTRKTTPGLRILEKYAVAAGGGCNHRFGLYDG
                     VMIKDNHIIAAGGIAQAVVMARAKVPHTLKIEVEVENLVQLQEALEAGADIIMLDNMP
                     PAMMKEAVTLVAGRALLEASGGIKAANLAEIAQTGVDFISMGALTHSAVSLDISFDLH
                     QL"
     gene            complement(24500..26098)
                     /gene="nadB"
                     /locus_tag="P5549_03010"
     CDS             complement(24500..26098)
                     /gene="nadB"
                     /locus_tag="P5549_03010"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRW12074.1"
                     /translation="MRITRYTWKLQPDTTIMHTDFLIIGGGIAGLFTALKASAFGEVV
                     VLTKKTIKDSNTGLAQGGIAAAVHEEDSPFLHLEDTLEAGAGICDIEAVDVLVREGPE
                     RVRELIAAGASFDMKDGSISLTREGAHSKARILHSADTTGETIRAALVKNCEADQRIR
                     IFEYQFLIDLLGSEEKECQGALVYDKQNGRQILYIARAVIIATGGAGQLYRYTTNPDV
                     ATADGMAAAYRAGCELSDLEFIQFHPTVLFSHDTQRFLISEAVRGEGGLLYNTNGERF
                     MNKYHSLLELAPRDVVSRAILNEMVLTGSEYVFLDMSIIPGVKERFPNIYRTCLLHGI
                     DLEKDKVPVSPAAHYTMGGIKTNTYGETGVNGLYACGEAACTGVHGANRLASNSLLEG
                     IVFGQRIVERAGEIMYRRQVKAEELCRSFAAGGVYAPLHPQIGPVEIRQRLQDVMWEK
                     VGIIRNEEALKAANQEIEYLYNHLARGDDNLAFIEMVNMLTVAHVVVQAALWRRESRG
                     GHFRSDFPQRDDVRWLKHTSFVNC"
     gene            complement(26114..27019)
                     /gene="nadA"
                     /locus_tag="P5549_03015"
     CDS             complement(26114..27019)
                     /gene="nadA"
                     /locus_tag="P5549_03015"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRW12075.1"
                     /translation="MANDIIDYIMRRKQELNAVIMAHYYQNPEIQDIADFVGDSLQLA
                     QQASATSAQVIVSCGVNFMAESAKILNPDKMVLLPAQAGCPMADMVTPEALREMKRQH
                     PQAVVVAYVNTSAAVKAESDICCTSSNALAVVNSIPREKEIIFIPDRNLGRYVAAQTG
                     REMIFWPGYCPVHDIVSAGHVKEQMSLHPQAKVVVHPECPPEVIALADAVRSTAGILQ
                     YVQASAEKEFIIGTEEGFLHSLRKACPDKTFYLARSHFVCQDMKMISLSALALSLEKL
                     QEQIEVTENVRIKAFRSLQNMLEIK"
     gene            complement(27035..27406)
                     /locus_tag="P5549_03020"
     CDS             complement(27035..27406)
                     /locus_tag="P5549_03020"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRW12076.1"
                     /translation="MLRYMLKSKIHRAVVTDANLNYVGSITLDRDLMDAANIIENEKV
                     TIVNNNNGERFETYVIEGERGSKTVCLNGAAARKVQRGDVVIILTYTILSDEECQGFK
                     PVLVYVNEHNEVQKISPGGMA"
     gene            complement(27410..28258)
                     /gene="panC"
                     /locus_tag="P5549_03025"
     CDS             complement(27410..28258)
                     /gene="panC"
                     /locus_tag="P5549_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRW12077.1"
                     /translation="MLVFNEIKEMQSWCLEFKRQGRKIGLVPTMGYLHEGHLSLVKEA
                     GRQCDIVVVSIFVNPTQFGPNEDFDSYPRDIESDRQKLAQEKVDAIFAPSAREMYHEG
                     YNSWVELKGEITSKLCGASREGHFRGVTTVVGKLFNICQPDKAFFGQKDAQQAMIIEK
                     MVQELNFPLAIVRVPIVREADGLALSSRNVYLDDEQRRQALVLSRSLQKARQLAENGE
                     HNVARLKQEIRKMIEAAPQARIDYVEIYAADDLGDVEEINGRVLIALAVKFGNTRLID
                     NLIVEV"
     gene            complement(28327..29157)
                     /gene="panB"
                     /locus_tag="P5549_03030"
     CDS             complement(28327..29157)
                     /gene="panB"
                     /locus_tag="P5549_03030"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRW12078.1"
                     /translation="MSKVTIATIKDKKAKNESITMLTSYDYSMAAMVDEAGIDMILVG
                     DSLGMVQLGYENTLAVSMDDMVHHTAAVVRGTKNALVVADMPFLSYHISREEAVRNAG
                     RLIQEGGAQAVKLEGGLERVDFTKAILDAQIPVMAHIGLTPQSVNAFGGFKVQGKDLE
                     SAQNLIAAAKAYEEAGAFSVVLECVPTLLAKKITESVSIPTIGIGAGAYCDGQVLVVN
                     DMLGMFKGHIPKFVKKFANLEPLIKEALQAYKQEVEAGTFPGEEHGFTIKEDVLERLY
                     "
     gene            complement(29154..30068)
                     /locus_tag="P5549_03035"
     CDS             complement(29154..30068)
                     /locus_tag="P5549_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRW12079.1"
                     /translation="MLRKKIGIIGAGVVGTAIGVVLHDKGYEITGAFDIKPESTELLA
                     KRTGCTVAANPEDVARAADLLFITPCDSAIQKVVDTLADRRAIHSGHTLVHMSGAQSS
                     EILDRAKEFGARVLSVHPIQSFASPDMAIKNLPGSVFSIEGDRQAFDVAVCIVETLGG
                     EYFFIDRKAKPLYHAGACVVSNYLVTIIDFGVKLLESTGIPKSMATRALLPLINGTLH
                     NVENIGIPKSLTGPIARGDLSTINKHLDCLEKMAPELTKLYGWLGYYTAQIALEKGSI
                     DKEAMEKFQTVFMQELARISSVSKEVRQ"
     gene            complement(30184..30690)
                     /gene="folK"
                     /locus_tag="P5549_03040"
     CDS             complement(30184..30690)
                     /gene="folK"
                     /locus_tag="P5549_03040"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRW12080.1"
                     /translation="MNGKVFLGLGSNLGNKAVNLAQAVELLAHHDKIKVTRQSSLYIT
                     APWGKTDQDEFLNQVVEIDTELTPLELLDVVQQIEIKLGRLRNEKWGPRVIDLDILLF
                     GEETIALPELVVPHPHMYARLFVLVPLQEIAPLLVFPDGKGIGEVLARAAQLEDASQS
                     IRRTEIII"
     gene            complement(30739..31098)
                     /gene="folB"
                     /locus_tag="P5549_03045"
     CDS             complement(30739..31098)
                     /gene="folB"
                     /locus_tag="P5549_03045"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRW12081.1"
                     /translation="MDKIMARGMTFKGCHGVLDFEKTQPQTFKVDADLFLDTRLAGHR
                     DDISHTVSYADVFALVKNIVEKESYQLLEAMAEKIAAAVLEQFPVEGVEITVYKPEAP
                     VEGEFDYFGVSIARSRN"
     gene            complement(31091..32284)
                     /gene="folP"
                     /locus_tag="P5549_03050"
     CDS             complement(31091..32284)
                     /gene="folP"
                     /locus_tag="P5549_03050"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRW12082.1"
                     /translation="MVHHAVLIENRAEAKQYLEEIGVSSPGIAYMVDKAVFRCIKLKH
                     ISHRAANILKQEMLAKGGEAAVTRDAAGGEKGFGDVLLLGTLKHYTLLLEKLKQQPFG
                     LRTVAAEIENILQTMEAPLSDLALAQGKNLALGSKTVIMGILNITPDSFSDGGRFLEP
                     DKAYARASTMQAEGADIIDIGGASSRPDSIMVSEAEELARVIPLVKKLVDAGMLVSVD
                     TFRAEVARQSLENGAHLINDIGSLQMDSELLDVLIKWQAPVVLMHNRLQLRRHEPYVN
                     LIDDIAAELAQSAGQAEEAGLGPGKIILDPGLGFGKSPAQNRLIIKRLGDFKSLGWPL
                     LIGASRKSFIGKTLGLEVEERLEGSLAVMAAAIMNGADIVRVHDVQESCRMARMLDAI
                     RSEHG"
     gene            complement(32300..32827)
                     /locus_tag="P5549_03055"
     CDS             complement(32300..32827)
                     /locus_tag="P5549_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRW12083.1"
                     /translation="MVKRIELLLQDEQYVDYMKRNKDDENVTAYCQHGLQHHIDVARI
                     AYILVLEHNDLNYFVRESGVSGKLAAKECIYAGGLLHDIGKWKEYREGVDHASFGAKL
                     AREILPRAFFNANETSLICRAIYEHRNISRDMSFLGERIHRADNLSRVCSQCEERNTC
                     PKRGNKEFSVTTFAY"
     gene            complement(32911..>33861)
                     /locus_tag="P5549_03060"
     CDS             complement(32911..>33861)
                     /locus_tag="P5549_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HRW12084.1"
                     /translation="MASTNRPDILDPALLRPGRFDRHILIDRPDVKGREAILKVHVKD
                     KPLDENVNLEILAKRTPGFTGADLANLVNEAALLSARRDKDSVGMEELEESIERVIAG
                     PEKKTRVISEKEKKLVAYHEAGHALVSFFLPHTDKLHKISIIPRGRAGGYTLLLPEED
                     RNYITKSYMLDEVTTLLGGRVAEALVLNDISTGAQNDLERASSIVRKMITEYGMSEDL
                     GPLTFGHRSEEVFLGRDISRDRNYSDAIAYAIDKEASHYMDTSYNKAEKILRDNIDKL
                     HHIAARLIEAETIDSDEFERIMNDEQQQTESAPATEETLP"
     assembly_gap    33862..33961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33962..34626)
                     /locus_tag="P5549_03065"
     CDS             complement(<33962..34626)
                     /locus_tag="P5549_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRW12085.1"
                     /translation="MDKALKNIAIYTLIVLLALFAIKMTSTPQTEVRELTYSQFYSQV
                     EAGQVKNADIVIDDLVYKISATLKNGDKVTTVATKESNIDKIMQEKGVQVKAQPVPPA
                     PWWTTLLSTLFPIVILIVFLLFIMNQTQGGGGKVMQFGKSKARMMSGEQVKTTFADVA
                     GAEEAKEEMAEVVEFLKNPEKFIKIGAKIPRGVLLFGAPGTGKTLMAKAVAGEAGVPF
                     FSI"
     gene            complement(34730..>35231)
                     /gene="tilS"
                     /locus_tag="P5549_03070"
     CDS             complement(34730..>35231)
                     /gene="tilS"
                     /locus_tag="P5549_03070"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRW12086.1"
                     /translation="LLHKPGSAKILQLKKGIIINKSYDRIVFTSQWQETGSFCIEVGI
                     PGQVVLPNGNRYHFALQTSAGFKAAPGDICLDYDKLSLPLVLRSRRPGDMIVLTGSAG
                     HKKLKKYLNEQRIPVRERNQIAILASRGGEIYAMPGLYFCPTMAVNQQSKNILLISAE
                     EGGSPI"
BASE COUNT         8399 a         9172 c         7935 g         9625 t
ORIGIN      
        1 gatcatagta aagactcccc cggacaaatc cgggggagtc ttttgttgct ttagatattt
       61 agctcgctgg atatagctta gtcgacgact atgatagcca tagctgtatc tccattgccg
      121 ttaaccacat tgttatcatc aataacaata acgttatcgc cttcggcgac tccttccaga
      181 acctgcagat ccttgtcatc gctgttatcc agatatacag tgtcttcgga atcaatcttg
      241 taggaattgc cattcgaaag aacaatcgtt cccttgttaa gggtatcaag ggtcaatggg
      301 ctggtagtac cgtaagcaat tttcttggta atgttggtag ctttgccgcc actcagtttg
      361 taggatacta catcgccttt ggtcagaccg gaaacaatat catccacttc caatgcatcc
      421 atacctacga acttgatcca gtcatcagaa tctgaatact gttcatagta gatcgcatac
      481 ttggtatctg tagccagaga tgctgctgaa gttgtcggga ttacaatata ttccagttca
      541 ttggaactgt tggtatagta tgagtagtat tttgcaccgg cagattcttt cagttctttc
      601 caacccataa cttcggtatc ggccagatcg gtcccaacca gattaaagac gactacatct
      661 ttcgcttcat accaaatacc gtcagctgct ttgaacagat cttcatcggt atccatattg
      721 gcattagcaa cagttgttgc agcataacca ccccagtatt tgatggtttt gataatattg
      781 tcggaatcga gagatatgct caccagaatg ccatcggtaa atgctccggc tgacgttccc
      841 ggtatcagta gtgcctcggc tgcagcataa tcggctgctc tgccgccgga agtaagatct
      901 tcgaccttgg tgcgggttcc ggtagcagca ttctgcagat agctgtcttc agctaatccg
      961 taggaatatt cttcaccgtc agatttcata atggttacgt ttcgtacggt atctccgttg
     1021 gctgcagtca tatctaagga ccaatcggta acaacaccat acatcttgga gctttcggta
     1081 tcgccgagaa taagtccggc taccttgccg gttgcactca gcaataagga gacatcagta
     1141 ttccaagcat catattcatt gtagagtaca ttctgggtca gagttgcata atcagggtca
     1201 tttgcatcat cagtataacg tccctgatat gtggtcttga gcagattgga ccacggagct
     1261 ttccaagtca tactcatgac tttgactgat ttgacgttgt tgttatcata ttgggcagag
     1321 gccagagtgc cattgatctt cgtactggta gctaccagat agtagtcaca accacgatag
     1381 ttttccagaa cactcagcgt gtcattttcc tgcagatcag ccagggtggc aggtttgcca
     1441 tctttttgga catagacgtc tttgtcagtg aaattggttg tattgacaaa tccatcccag
     1501 gaacctgagt tgctctgtac tttgaaatca atattcggaa gttctaaagc aacttcagtt
     1561 acagcagtgg ctgcaggcac accagcagca atcaattgtg ctgtaacaac agactgtaag
     1621 ggattgccgg cagcagcagg agttgcgcca gtctgatagg taactttttc gccgttaact
     1681 tttttaacaa taccaggggt cgggaaagtc catgctctta gggcaacgac ttcgtcatca
     1741 tcattcatga taacggcata ggcatcggaa gcatcataag ttttatctgc aatcttagat
     1801 gcatcaatgg tgttgagcag cattgcgtcc tgaacagcct gccattgtgc agttgtgctg
     1861 tctttggtgc caatagtagt agcgatagat tttactaatg ctgtcaacgg ggcatttatg
     1921 ataggagcaa actcagtgtt gcagctttca gttacaatcg cggtatcggc aaagctgtat
     1981 ttaactccgt cgatttttac tttgccgccg tcaattacga tttcatttaa gggagttccg
     2041 gcagcagtct tgggtccaaa tttcttgtaa tcttttaaca cgatgctggt gatttcatca
     2101 tcgtcattgg ttacaaaatc agcaaagttt tcaatagcgc ccagaattga gccaccgggc
     2161 agtgtgcatt cgtctgccaa tttctttgaa taaggctgat aaccgtcagt atttgcattg
     2221 gcatcgctca gattgccgtt gtcatcataa gtgtaatagt agatggtata ttcatcgtca
     2281 tcatttactg agaaagacat gatcagggca tcttcagttt tctgagattc actgaacttg
     2341 tcataaagca aggtatagct ctgggatatt ttccctttgc catcgccttt gtcataagtc
     2401 ttcagagatt cgttgaacag attgtcagaa gctgcatatt ctacaacgtt ttcatccagg
     2461 gttgcaccgg ctataacagc aacaatgcct ctggtggaag cctgaccggc aacgaaagtc
     2521 acatcatcca gaatgccaag attggcagct ttggcaacgt aatctgacgg ccattcgccg
     2581 gccagattgt cattgtagcc caaaagtctt aagagaacgg taactacttc agcttgagta
     2641 atttgatctt ccgggcggaa agttccagcc ggatcgcctt ttacatatcc ctgtgcagcg
     2701 gctacattga tccagccgtt ggcccagtgg tcagtggcaa catcgctgaa gggggaagcg
     2761 gttccgccca taccggaagc tacagatttc agcccggcag ttacgcaggc gattttcgca
     2821 aattctgcac gggtgatggt cttttcagct ccgaacgtgc catccggata accgtcaatg
     2881 attccaagac cattgagttt gtaaatagca gctgattcaa cggatgttcc agtaacatcg
     2941 ctgaaagttg ctgcaccagc gctgccaacc atggtgaaca taaatgttac caaaaccagc
     3001 agcatgatca gtttgttatg ttttttcaac atactgtctt ttcctcctta tgtttttaaa
     3061 tttagataat catattgatc gctcaatata acttcatcag gtaatcgtat tgatggctca
     3121 atacagcatt gaataggtga tctcggcgct tgtttgtgtt tttaattccc cctttcaatg
     3181 gtctcaaatg ttttgcttgg tgtgattttt gacctgttaa atatgacgac caaggcttat
     3241 atttggttcc ataaattaaa taaatatttt ttcattctgt ttcgttcgga ttcgacagcg
     3301 ttattgccat ttatatccta tgtcttggct aagtagcgac ttgcattttt gctggcgctg
     3361 gtgcaataat atctgagata ctacttcaat agctaaggag agggaaaatg aacaaacttc
     3421 agaaatatct atgcgcggcc atattggtgc tggtattggc cggcataggc tggcagtacg
     3481 ttgggtggca gaagcaggtc gagcggcatg aaaatgccct gcagttctgg caggtggaag
     3541 tccccaaaat acaagcggag tttcctgagt tcaagcctca gaatgcggaa cagatgatgg
     3601 cggcggaaaa caagcagttt gccgaacaga aaaagacacc ttacgtcaac tccggaatca
     3661 ttgtattatt gggattgatg gctggcggcg cagtcattta cgggccgatg atggtcgcta
     3721 aaataaaact tgctctgcaa ccccaagaag catccaagaa gcgtaaaaga taattcaaac
     3781 aggcgcctga tttaaatatc agtcgcctgt tttttgggcg aattggttcc atttactggt
     3841 atatgcttga atatgccgat taggacagat tattttgtcg gtaatttctt ttataacttt
     3901 gatatccgcc gacgctcagg ctaatcttgg agatccttcg gctttgcctc aggatgacac
     3961 taacggcttc agctcttcgg gattaaatat ttactgcttc aactcctcag gataacatgt
     4021 ttacctcttt ggcctctcag gatgacattc ttggctgttc ctattgctca ggatgacaaa
     4081 atctacagca tcagcttcac atacttctgt atgccttcgg caatggcctg ggcggctttg
     4141 gtctggaaat cggctgacgc cagcagttgg gcttcctcgg cattgctgat aaaggcggtt
     4201 tccaccaggg cagagggcat attgctctct cgcagcaccg catagttggc ctggcggatg
     4261 ctgcgatcag tacgtccgca tttttccagc atggcttttt ggagcagatc ggccagcagg
     4321 gagcgctggt attcctgggc ataaagggcc ggggtgcttt cgggtgcata atagaaagtt
     4381 ccggtaccgt gtggttcggt gctgtctacc gaatcgcagt gaatcgacac aaacaagtcg
     4441 gctttggctt tattggccat ggccggtcgg tcgctcaatc ccacaaaggt atcatcatcg
     4501 cgggtcataa tcactttata gcccgccttc tccaaaatat cccgcgcttt taagcccatg
     4561 cttaaaacta catctttttc gtacaaggtg cgtgatttgc tgatcgatcc ggtatcacgg
     4621 ccgccatggc cgggatcaag gacgatggtg acctggttgt cagtgtcatc agtgggtttg
     4681 gtcggttctt cctccatgac tttcttatct ttattgacca ggatgatgct gagttttaaa
     4741 ccattaacgc tgcgattgac ctggtaggtt tccaggtttt tgccggtgtt gactttgatc
     4801 tgcaatggct ttcctttgtc ttggctgagg ctgacccttc cggccagacc gctgaacaaa
     4861 tagtcgtcct ccacaaaacc ctgcccggtc ttgcttagat tcaaggtgat tacgccgtca
     4921 ctaaagcttt cctggtattc catagcagaa gtagcccgca aaacaatgat ctgattgccg
     4981 tttttaagtg tgatatcttc gaccgtggtc agttgcgagg ttattgtaaa gacttgcttg
     5041 cagccgttgg cttcggtggt gttggtatat tgataggcca agggcatttc cactgtcacc
     5101 caggtggcat cctccatcga ttgggcggag atggtcactt tggcgttttt gccgctgccg
     5161 atgtagaaat aggcttcatc acgggtggat ttcagcaccg cctggtcaaa cttataggtg
     5221 atctttttgc cgtcgcggct ttcggtgatg atgggttcag ccttttctcc ggttttcata
     5281 accagccggt aaccgtcact ggccaagcgg gtggatatcg taatgtatcc ttcggcgact
     5341 gcgacgtttt catctccgtg ccggttgatg tctgcttcat cgatgactac cggttcttcc
     5401 tggtcattgc cggcagccgg ttcagacggg gctgctccgt tgccgggttc agtggtgcct
     5461 cccactgagg catcggtcag ggggtccacg acccaggccg ccacccaagt atttctgcca
     5521 ccgcggctga cctgatacca gctgttcttt tctgccagca cggtcatttt ttctcctgcc
     5581 tgggcaacct caacaatggc tgcttcggtt gacggattat cgcgtagatt gacagatccg
     5641 ccggtcacta aaaccgctgg cggcaaggct ccggcagtgt gggatatatt gatcacccgg
     5701 gtctgttcat tccaatccac cttgccccca aaggcttcgc ccacaaaacg aaggggtgcc
     5761 agggtgcggt cattaacgat cttggcgggg acatccaatg gccagacttg accgttgacc
     5821 gtcggactgg tagagccgat cggcagtacc accgtggtgt tgctggtgcg ggcggtaacg
     5881 gtgcgggtgc tgtcattcca ttccacattg gctcccatgg cttcaaagat ggcccttaag
     5941 ggcaccaggg tacggccatt ttcaatgcgc ggctcgactt caaactgcaa ctggctgcca
     6001 tttagaatga cgctgggatt ggccggagca gaggataccg gggaagggtt ggtcgctgcc
     6061 ggcacagtcg tcaccgtttc ggttgtggtg gcggtaatat cgaccagggt caccagccag
     6121 ccggccacaa aagccaggct gccgtccggc agttggatcc ggtaccagcc attttgttcc
     6181 cccaatacgg caaaaaccag tttgtctccc agactggcca ctatatcata gtctgttccc
     6241 ggtccgctgc gcgcgttgat gggaccattt ttcacctgca cctgctgtct ggcggcgatc
     6301 agctcacggg caatgtaacc ttttaggctg ccatacttga tctggtacca atcgccggaa
     6361 agtgcggtta tctcaacttc cgtatccttt aatacctgtc caataatatc cccattgctg
     6421 ccgggagagg agcggacgtt gaccactgac ccggtcacaa taccgctcgc cgctcctgcc
     6481 ttggcagggg tcatgctgat caacagcagg gtcagcagca gaacgggcag caaaagacgg
     6541 cttatcctat gtaataaagg ctgggggtta ttaagcatga atgcacctcg caatttacgt
     6601 tttaatgaaa cttcgacttg tcgttttatc aatcctgctg ttaaaacaag gaaattcaag
     6661 gaaactgtcg ctatatgtat atacccacaa cggctggttt tctttcatga atcgcagaaa
     6721 atattcgaga ctactgaaaa agtacccgtt ttgtcatcct gaggagccga agcgacgctg
     6781 ctgagggttt tgattccccc tacacgaacc aagtcgttca ggataacaat aatgtcaatg
     6841 aaaaaaggga accggctcga ttaagctgga attccctctt taatccattt tgtcaattgc
     6901 ttgatcatac tttgttctta acgccgtgcc cggcgctggg cagtcggctg cgttcccagt
     6961 taagttcttc aatggtttgc tccgcttcgc tttcgctgtc atctttatct ttatttttat
     7021 ctttaacact gctttgattg ccgggattgt aggcaaccgg tgaatcggaa acgactgcca
     7081 ccgtttgtcc ggccagcatt ttctccagca aatcctcggt tttatcctgg tcagccaccc
     7141 agtaactcat ggtcagattg ccgtattcat cccgctgatc ccaaaaatat cccggcagcg
     7201 tctgggcaat aatggaatcg ctactgaagc ccggcgccca actggccatt ttcagcatgt
     7261 ctgtcatgcc cagattggta tcgatatttt gtttgaccac cttgaccaga gccggtagtt
     7321 tgacaatatt tttggcttgc aacaactcgt ctcccagagc cttcaggaaa atctgctggt
     7381 gctcggtgcg ttcaatatca cccagggcat aatcacggaa acggacaaaa gccagggctt
     7441 gactgccctt caaccgctgc tgaccgggct gcaggtcaat gtcttcggag ggcttgtaca
     7501 tccgctgatc aacattaatg tctaccccgc caatggtatc aatgatcttt ttgaatcctg
     7561 caaaatccat ctctacataa tagtcgatct tttcgcccac gatttcttcc actttatccg
     7621 ctaccagttc cggtccgcct accgcgttgg cgctgttgat cttgtctgta ctgcccttaa
     7681 tcgtcacacg ggtgtcacgg gggatggaga ccagcgccat cttgttcagt tccgggtcga
     7741 tggaggctag gataatagta tcggtacgcg tgtgggtctc ccccggacgg gcatccgttc
     7801 ccagcagcag aatattggtt ctttccccgt cgatgacggt tggttcctgc tcttcggtgg
     7861 gatcatcgtc ggccagcagc agattgtgca aggaagcgcc cagcccaaaa gacagcatga
     7921 aaaccaggct gaaaaccaat accagtttta ataacaattt tagcttggac atggtaataa
     7981 cctcaaactt tccaatctca ggacgaaatc ccggtgcttt gatcggtgct gtgcaccgag
     8041 gccttggtct gaccggtcag catgccgatg cggtttgccc cgatgaccat aaacagcagc
     8101 aggaaggcca gcaccaaggt cgctttggga ttgtcaaacc agctcagcac aatcgcggtc
     8161 aaggcaaaaa agccactgat cgcgtatatg atcagtaccg tggtctgatg ggaatagccc
     8221 aattttaaca gccggtgatg caaatgatcc ttgtccggca tgaatatcgg agttttctta
     8281 taaatacgcc gaaaaatcgc aaaaaaagta tcaaagatgg ggattcccag gatgacgatc
     8341 gggattacca aggaaatcaa agccgtactc ttggcaaccc cggtgacggc cagacaaccc
     8401 agcacaaaac ccaggaaatt ggaaccgcaa tctcccataa aggttcgggc aggatggaaa
     8461 ttatagggca ggaaccccat aatggcagcg accagggcaa aagcaattat ggccgtttcc
     8521 atctgtccct gcataagcgc cacaatgccc atggtcatac cggctatgcc ggctactcca
     8581 gccgccagtc catccaggcc gtcgatcagg tttatggcat tggtaatgcc cacgatccat
     8641 aaaaacgtca gcgggatact taaatatccc agcgccagca taccgtcaaa gggattgctg
     8701 acaaaatgga tcaccactcc aaaatatacg gctaccgcag tcgcgatgat ttgtccggcc
     8761 agtttgatcc aggctgacaa ttgataaaca tcatccaaaa tcccaataat aaaaataatc
     8821 acggctccaa ttacaatacc cacataaggc ccggaaactt tcactcccaa aagcacacaa
     8881 atcataaaag caaaaaatat agccagacct cccatgcggg gcatggcagt ctgatgaacc
     8941 ttgcgcttgt caggctgatc tatggcaccc agacggtgag ccagccggat caccaacggg
     9001 gttgaaaaat acgcgattac aaaagccgac accaatatta atgccgacgt aagaaaagac
     9061 atttctatgc aatcctcccc ctctgttttg tctaggtcaa ttctatcacc gctctggtgg
     9121 cttgtctata taccaagatt tagtgatttc gggcttaaat aaaggaactt tgcctttaac
     9181 tgtagaaaaa aaccacaagt tctctataat aataacgtat actatcattt agttgagcat
     9241 ttttagaaag ttagaaagtt aggtgcctgg cacgtgaaaa ttgcgctatc tggatattat
     9301 ggttttgaca atgccggcga cgaagctttg ttgctggcca taagctcgtc gatcaaaagt
     9361 ctggtaccgg atggtgagtt tgtggttttt tccggtaatc ctatcaagac aaggcagttg
     9421 cacaacattg gggctgttta caatatgaat ccctggtcgg tcgcccggga gctgttgagc
     9481 tgtgatctgc tcatcagcgg aggcggtagt atttttcagg atgtgaccag ttcccgttct
     9541 cttctatatt atataagcgt tgttgtactg gctcgcctgt tgggtaaacc ggtggttttc
     9601 tatgcccagg gggtaggccc gattcgcaaa ccttttaacc gctggctgct gggccgggtc
     9661 gccaatcatg ttaacctgat caccctgcgt gaccaggcat cccgtgaatt cttgcgtaag
     9721 ctgggggtca gcaagccgcc cttgcaagtt acggctgatc cggtttttgc cttgcagacc
     9781 aatgaagata tcaaagctga tatcaagcat cgttatcctt tattatcaag cccggcaacc
     9841 aaacggctgg gggtttctgt ccggcactgg ccggcgctgc aaggttatca acagaaattg
     9901 gcagttgccc tcgatcattt taagcgtgcc ggatatgaaa ttatttttgt ccccatggat
     9961 tatcctgaag atatcaagga atctgaacga gtcagcgctt gtatgcagga agaagcttgg
    10021 atcatcacag atgagcttac cactgcccag catttggcgg tgatcgccca gctcgatctg
    10081 atggtggcta tgcgtctgca cgccttaatc tttgcggcca gctgcggggt gccttttgcg
    10141 ggtatatcct atgatcccaa gattgatgcc tttttggatt tattcgacaa acggcctctg
    10201 tctccatcaa gcgagttaat gatcgctcaa attgaggaat tgctgtatga tcaaaatgcc
    10261 caggatcaag ttcgtcaccg ggcagataaa atgcgcgctc agtctttgga aaacgcccgc
    10321 atgactttgc aggttttaaa aaattcgcga cgttaaaaat agattgcatc tgcttggaag
    10381 cttatcccag cggtcataaa gatattttta catagaaaat caaaaacaga aaatgtttca
    10441 cgtgaaacat ttctgacaga tttgaggatg aatatggagc aggaaaacac tcgcaccggt
    10501 aaaactttta ttggggtctc cctgatgatt tttatttcca aagtttttgg ctttgccaga
    10561 gacattgtgt ttgcatccgt ctttggaacg acggtgctga ccgatttgtt tcaggtcatc
    10621 ttcagtctgc ccagcctgtt gttttccagt atcggcaatg ccctatcctt tgtaaatatc
    10681 cctgatctta cctattatct gaaacatggc accagagagg aaagaaacac ctatctctcc
    10741 aatctctatg cccaggtggc tttgggggcg gccttgctgt cattgctggg tatcatcttt
    10801 gccccggcgc tgaccaaatt gattgccccc gggctgcaag aagaagttac ctccatggct
    10861 ttgctgctga cccggatcat gattcctacc ctggtgtttg tgacgctcac ctatattacc
    10921 gccggtgtcc tgcaggtgca cggttatttt ctgctctccg ccgccatcag tatccccttt
    10981 aatttcttga tcattgcctc gttgctcctc aaaggtcagg atctgatctt ccttggcatc
    11041 gtcaccactc tgggctggtt tttgcagttt ttgattcaag tgccggtgct gttcaaagaa
    11101 aaatatcagt tttccctaaa aattgattgg cataatcagc ataccgtcaa tatgttcaaa
    11161 cagctcctgc ccatcctgat gggcaattcc ctgttgcagc tctgcctgat catagatcgt
    11221 tcctttggca cccatctgga tgaaggcacc gcggcagcgc tgggctttgg cagtaatctg
    11281 tttatgacca ttaccggggt gttcatcatc gcgttatcca cggttatctt tccccgcctt
    11341 tcccaatact gtatggacag cgattaccgc caggtacgtt ccatgctgcg ctatggcttt
    11401 aagatcctgg cctttattct gctgccctat ttaattctgg tcaccgccta ttccccggac
    11461 atcatcgccc tggtctatga acggggggcc tttacctcaa actccaccca tctgaccgct
    11521 acggcctttc tatgctatag ttttgcggtc gtgggatacg cctgccagga agtgttcaac
    11581 cgggtttttt atgccctgaa gcgctttaag acccccatgt atgccagttt ggtctgcctg
    11641 acgctgcatt ttgtcctcaa cctgctcttc taccgtcagg cgggtatcgc cgggctggcc
    11701 ttgtcaaccg ctgtttgcct gctggtctat gccttgatta tgggcttgat cgtgcggcgg
    11761 gtgatcggcc ctttcctggg tcaaaatttt gccaattact ccctgcgttt gtgtctgccg
    11821 gcagccggta tgattgcaac cattctggtt tgcagatacc tgtttgtcag tggactgtta
    11881 ctttcatttt tgcttccctt actggccagc ggtatggttt atttgctggt ggcctggctg
    11941 gggggttttt accaagaact tatcaatagg ggggatcctg tctgatgaat aaccgtgaac
    12001 atgccttttt gtggctggcc ctggccgtag ccttgctgtt ttccataacc ggcttttggc
    12061 tgcggatcag caatgagagt atgaataaca ccttggtaac ggtggctgat tatcagctct
    12121 ttgagaaagg cgctcaatcc tccagcctat ccatggatga ggttctggcg gatctcaaag
    12181 ccagcggggt taaaacgatt gcggtcaagg aaacgaccct gcgcggactg gctgcccggg
    12241 gtgacgccta tgttgcttcc ttcgctgatt tcaacaacac ggttcgcacc tatcagccgg
    12301 atgtctgggc aattgtcaaa cagctgcccg atatggagga aataagtcct gccaatctgg
    12361 tcgtttcggt tgaatccccg gaaacagcca gctttttaca ggaaaaattg ggcaaccgct
    12421 ttacgccaga tcaaatcgtt aacttcccgg cgggcggccg ccaatacttt attatcaaag
    12481 cggaattgga agcattggac aaatccaaaa ccaacatcag agaatatgat gtgcaactgg
    12541 ggtttgaaac atcgatcctg caaaaattaa aagcaatggg gtttgcgatt gttttacgcc
    12601 ccggcgacag caacggaagc aatcgtgatt atttgcagga atacgagccg ctggttcgcg
    12661 cttatgatgt caagaccttg atctttaatt acaatcgtgt cagcggcagc ccggatcact
    12721 tggatgtcat ggcccgcttg attgataaat atgatctgac catcggagta atcgaaacct
    12781 ccaagcaact gggtatcatt gaacaggacg gtctgaacga ggtcatgtct gccaccaaat
    12841 acccgatcaa tagggtttac tcgaccggca atgatgattt tgtgacttcc gtggacgaac
    12901 gttattatcg ctggatcagg ggagtcgttg atcggggcat aagaattttg tatgtcgtac
    12961 cttttaacga tgataagaaa atagcctcgg tcaacgtgag tgaaaccatt gatacggtag
    13021 ccagatttct gccggcgatt acggccaagg ggtttgtggt cgaccagacc gtgctacctt
    13081 atggtctgaa cagtgaaacc acgacggctg ctcatcgtct ggccatatcc ctcagtctgt
    13141 tggcagcttt gctgctctat ctcctgtatt tgtggcatcc gcagcgccat tggatgcggg
    13201 ccattgccat tcttggcttg ctggcctgtc tggctataaa tattgtgctg cacatggact
    13261 tttccaaaat ctacgccctg gcagcggcag ttttatatcc ggccttttcc agtttactgt
    13321 tgctgattta tttaaaaaaa tacccccgcc atgggttgat gaaaaaacta tggcgctccc
    13381 tggccatcat cctggtgatt aacgctctgg gcatgtatac gatcgtcagc tctctggcgg
    13441 atatccgttt catcatgaat ctgtcctatt tcagcggggt gaaggtggct tttattaccc
    13501 ccttgctgtt gttcccgctc aattatttct gtgtcactgt cccccgggaa aactggctgc
    13561 cttggttacg ggcccggctg aaagaaagcc cctcctatct ggcgctggct ctattcatgg
    13621 cggccctggt ggtggtctat gtctatatcg ggcgcagcgg caatgattct gggattcagg
    13681 tttccagcct ggagatccgg ctgcgggaga tactggaaaa tattttcctg gcccgaccgc
    13741 gtttcaaaga atttgtgatc ggctatccgg cactgatggc catggtttac ctgtatcatc
    13801 gctataaaaa cgatctgatc ctgttgttgc tgggctttgg cgtggtcatc ggcagcatct
    13861 ccatggtcaa ttccttttgc catgtcttta cggcggtaag catttccgcc agccgcaccc
    13921 tggccggact ggccaccggt ttgttgctgg gcacaatgct ggttattgcg atttggctgg
    13981 cggaaaaaat ctgggccaaa tatggctttg actgagaaat aaaagtttgg ctttgatccg
    14041 gttgaaagag atcctctctt cggaggacta cccctatgaa ctaaaagtca aatattaagg
    14101 cagctgcgct gcgcgcagga tgacatgcta aagtaaatct acagttttaa aaaagattat
    14161 gcaaaactct cattttaaac aactgaaagg aatctccaat ggataagcaa catctgcagg
    14221 agtttgattt tctgcgggct ctggccactc ttaccgtgat cggtatccat gtcagtgcgg
    14281 cttatgtata tacttccatg tggggctgtc tggccaacca ggtagcccgc tttgcggttc
    14341 cgcttttttt gatcatgtcg ggttttttat tgttgcatgc cgatcttaac aaacaaactt
    14401 tttccgcccc ggatttttac cgccggcgct tcagcaaaat tctgtggcct tacttcctgt
    14461 ggagtctttt ctacgcttgt ctgaacggaa ttttgggagc cagtttgctg gctgcgttgc
    14521 tgcaactgcc gattcagctt ttatggggaa cggcttttta tcatctatat tttgtggtca
    14581 tcattatcca gctctatctg ctttatccct tgctctaccg ctggatgatt cgtgcccccc
    14641 gcagctgttt gctgaccgga ctggccctga ccctgctttg ccagattgtg ctcaaccgct
    14701 attcgcttgg acagttggcc attccctcgc agctcaataa cctttccctg gtctttttcc
    14761 cggtatggat ctttttcttt gtttttggta tgtttgcggc actttgtaaa gaaagactgg
    14821 tgaagctgtt taaaaaccgc cgcgggctgt tggctttgct ctggatcctt agcctggcgc
    14881 tggtggtcat cgacggttat ctgcataata cctatggttc ttccagccgg cctacggtca
    14941 cgctgtatac cctgatcagc tttttctttt tctatgcgct ggcccttcat ttgcggggag
    15001 cttttcgccc tgtcattgct tggctggccc ggcagtcatt tttaatttat ttgctgcatc
    15061 cgctggtgct taccggcttg atcactttcg ctgcctggat cggccgggct gacctgtgga
    15121 accgcacccg gggcatgatt gccatctatg gattggttac cctgctaagc tgtgcagccg
    15181 tatacctggt ctcgctgacc cctctggcag aaaaactagg cggggtaaga agaacgaaat
    15241 cataacgttt tactccctaa ggaggattgg cgccatgaaa aaagtttttt atgtcctggc
    15301 cgggatggtt ttcctgatcg tactggccgc agccggattt tattgggcca ggtattatga
    15361 tcactatttt gatgaaaaaa agatcgtcag cgcggatgaa acgaacacca ttgtcagggt
    15421 tgataaagaa tatcggcgtt tatataatca tgccggcggc tatgccatca attatccggc
    15481 gcaattccgg cttgatgtta gagaaccgtc gctgaaaagc gatttcagcg acgatcagca
    15541 caatatcgaa gtctacagtg ataacttcac cggcaaaatc agcgacgcca aagcctatat
    15601 cggttacagc aacggatttc tggcccgtga taaagatatc caggtggaat tcaacaagta
    15661 ttctgtcatt aacggacgcc tggctaatct caccaaatgg cagcgggcca agctggcccg
    15721 ggttgaaaat gataaaaacc attacttttg tatcaaattg gtggagaatt cccatcaggt
    15781 ttataccatc gtgataaaat ccagccagcc catcgacgac cgggagttca tgaaactggt
    15841 caagagtttt cgtcccattg aaaagaataa tgacctggcg ggaatcgtct atcagcccca
    15901 gcctaaaaac tggaattcag aaaccacaac ggtgtacgat cagtatttcg gccatcaggc
    15961 tgatttgaaa tggggcatct atgagccaac cgcccccgct aaaatgaaaa atctgacctg
    16021 gctggaagac cgcctcgatt atgattttca ttttctgatc cgttaccaga acctcgacaa
    16081 acctttgccg gtgcaggaat tgcaaagcgc ctgggccgat cagaagttga tcgaactgac
    16141 cttgcagctg gggtttgaat ttgccgaggc ggctgccccc tataacccca agggaatccc
    16201 cttggcccag tttaatgaaa aaatcattcc tgagctattg aacggtactt ttgatgctta
    16261 tctggcgcaa tacgcccagg atataaaaga cttcggccat cctcttcttt ttcgcttgaa
    16321 taatgaaatg aacggggact ggtgtgttta ttgcagttat cattacggca acgacgctga
    16381 tttgtatatc gaagcctgga aatatattta caagattttt gccgaccatc aggttgacaa
    16441 tgtcatttgg gtatggaacc cccatgacct gtccttccct gattttgcct ggaatcatta
    16501 tctgaattat ttcccaggca gcgaatatgt ggatattatt ggcctgaccg gttataatac
    16561 cggcacctat tacgaaggtg aacgctggcg ggaatttgct gcaatttatg acccgctcta
    16621 tgcagaatat gaaaagcgct tcgctctgcc ttttatgatt accgagtttg gcagcaactc
    16681 cgtgggcgga gacaaagcgc gctggctgca ggacatgttt gcccacatcg atcgttatcc
    16741 gagtatcaaa gccgctatat tcttcagttc tacggatttg gacagcaacc aacagccggc
    16801 ccgcatctac cggatcgatg aaaccgaggc tgttctcgac acagtcaagc agggactggc
    16861 ccgttatcaa tagatttatt cttgcggcaa aggatgagtt actgcgataa aggtcggaaa
    16921 taacaaaaat caaggccatg tttcttttca tgaaacatgg cctttactta atttattcac
    16981 caatcattcc agggatgata aaggctatac ccccatcgtt ttgccgtcag ccaatgctgc
    17041 ctattcccgg gcccgcagag tgggaaacag aataacatcc cggatggagg atgaatcggt
    17101 catcagcatg accagccggt caattccgat gcccagcccg ccagccggag gcatcccgta
    17161 ttccagggca ttgatataat cctcatccat catgtgagcc tcggcatccc cgccggctct
    17221 tttttccacc tgttttagaa aacggttttt ctggtcgatg ggatcattca gttcggaaaa
    17281 ggcattggcg atttcccgct gcatgacaaa cacttcaaac cgatcagtaa attccggatt
    17341 ctgcgcattg cgcttggcca gcggggaaat ttccaccgga tgaccgtaaa caaaggtcgg
    17401 ctgaatgagt ttttcctcga cgtattcctc aaatacttca ttgataattt ctccgcgggt
    17461 actgcgatga tcaactttca agcccagcct ggcagcagct gtgcgggcct ggtcatcgcc
    17521 ggaaataacg gtaaaatcta cgccggcata ctctttgatg gcatccagca tggtaatccg
    17581 attccaggga ggtgtaaaat ccaaggtaac gccctcaaac tccacctgca tgctgccgtt
    17641 aacctgctgc agaacctccg ccaccatgtt ctcggtcaac tccatcatga cctgatagtc
    17701 tgcataggcc tggtaaattt ccaacatggt aaactcgggg ttatggcggg tgtctatgcc
    17761 ttcattcctg aaactgcggt tgatctcgta gaccttttcc agcccgccca cgatcaagcg
    17821 ttttaagtaa agctcgggag caatgcgcag atacagattc atgtccagcg tattgtggtg
    17881 ggtaataaaa ggacgggctg ctgctccgcc ggcaatcggc tgcatcatgg gcgtctccac
    17941 ttccagaaac ccccgggcat caagataatt ccgcatctct ttcacgatgc ggctgcgttt
    18001 gatgaaaacc tctcgcgcat ccggattgac gatcagatcg acgtagcgct gacgataacg
    18061 catttccaca tcccgcaggc catgccattt ttccggtaag ggattaagtg atttggacag
    18121 atacgtcata tcctcaacat ggacggtgat ttcacccttc tgggtgcgaa agacatagcc
    18181 cttgatcccc aatatgtctc cgatgtccag ctttttaaaa agttcgtagc gttcttcgcc
    18241 cagatcatcc tttttgaaat atagctgaat gttgccggac agatcctgta aattggaaaa
    18301 tcctgccttg ccatggcgtc gcttggacat gatccgtccg gctacagtga ctttctgtcc
    18361 ttccaaagcg gcaaaatcat cgataatcgt ctggcagttt gtatcgcgtt caaaacggct
    18421 cccgaaagga tcgatcccca tatcgcgaag ttcctgcagt ttttccagac gtactctttt
    18481 taactcgtgc agatctattt cctgctggtt ttgctccgac atttgtccac ctctatctga
    18541 ggtgccaggc accaaacaat ctaacttgct tgcatgtaag ttagattgtt tggtgcctgg
    18601 caccaccttt ttatctcttc acttttatga ttttgtattt aatgatacct gccggagctt
    18661 cgacttcgac cttggcccct accgctttgc ccataatggc tttgccaacc ggggattcgt
    18721 ctgagatctt gccatccttg agcttggatt cgactgacgc gaccagcgta acattgactt
    18781 cacgtcctga tttcatttcc ttcagggtga ctttggtccc cagagaaaca acatcgtcac
    18841 gtatttcatc ggcagtcatc aagcgggcat tcttcaaggt tttttccaaa gtcagaatac
    18901 gtccctcaat aaaagcctgt tcgttcttgg catcttcata ctcagagttc tctgatatgt
    18961 caccaaaagc gattgcctgc ttaatccgtt ccgctacttc ttccctccgg acagatttca
    19021 gggtttccag ttcattctcc agtttttcca gtccctcttt ggtcaaaata atttctctgt
    19081 cttctctttc ggccatttaa tcgctcccta accataaata ttgtagcaaa ccaaatcatt
    19141 attgctgcaa ttaaattttt aaaagcgagc cccgctcgct cccggaaata ataaagggtt
    19201 atcttcctca cccttaccct taatccttta tggtattata atgaccccat atagcgttgt
    19261 caagaaatcc tccgccataa ccttgtcgaa aaggttccca aaggcccaat ttctcaccgg
    19321 cttcgatgag aacagttaaa tcagcaccgg ttttgatgac cggtgcaaaa tgcaagtttt
    19381 gtaattctac attcccatat gaaatacctg ctttggtgta cagaatactt tccagaatcg
    19441 gagccaggat tgacaaacct gcctccatgc cggcccgttc cagggcattc tccaggctgg
    19501 gagccagatg gcggccttca tcggtcagtt ccagccaaaa aacttccggt gaagtcactg
    19561 ccaaacgttg aaaaccgggt tggaaaagca cgatcaagtc acctttttgc catttttcag
    19621 gacaaatata gctgttgctg atcgcccatc cccgctcata cagtagacgc caggcaatcg
    19681 cttcatcacc gcttggatgc tgattctgca ctgcaatcgg caagccgcag ctgcccagat
    19741 gatccagtaa cggaagcaac gctgccatat ctcccaccag gatcagtcgc ttgacgtctc
    19801 ttttttccag gatgtatttg accatgactg ctgccagggc ttttacaaaa tgatctccaa
    19861 accataaagg aaattgggct tcaccgggct gcaaggcaaa cctcagacgg tggtctgcag
    19921 ccaggctggt aactgcgaag tcctgcataa ttccctctga ttttttccac atgcagccgc
    19981 gctgcgcaga ctccagctgt tgccagtttt ccggccccaa gggcaaaaat acattgcatc
    20041 ccttgaaccc cagatctcca aaatggatca gatgctccac ccggggagag aacagaaccg
    20101 gggccaacct gtccaaaaaa gtttttttgg cggtcagacc ccggcgatta cgacttaaca
    20161 tatgcaaaat ggcaaaatca aattcaggca cgcaatgtct ccaaatcaac caccccgggt
    20221 ttgaaatcaa gctccttcag catggcataa taatcctgcg gactaatctg gtcaactggt
    20281 ttgttcagca aggtaaccat caatgcctca attacattgg taccgaaaga acgtcctccc
    20341 atatccggcg tggtagtaat cactttgctg atgccgcgtt gcttcagcat caccatatct
    20401 tcatgagtcg tcgtattggt gataatcaac tggccgttta atttttcggg taaataacgt
    20461 ttgatataca gaaaatcgcc ggccagcact tctgcattat aatagtattt tgagtgtttg
    20521 ggaattatga catcctgatt ttcaccggtt gggtacagct tgtcaaacgg catgttgacc
    20581 acgatcggag ctgccatacg gccaaaaatg cgcagtgttt tcagagaatg gatcggaaag
    20641 gggatcccca gggtataaac aaagtcaccc agcgtaagat cagccccgac ttcttcaaag
    20701 gcttccgcca tgccaaagcg atcaactccg cacaccatca ggactttttt gccgtgcaga
    20761 tcataaatcc cttccttttg tacatcgata atgcatttgc gttccaaggt attcttcaaa
    20821 ccgctgccgt cgaccatggg ggtcttgcgg gcagctgcca cgagtttttt cgcatctttg
    20881 atgacataac gcttgcccgc gatctggatg tacagatcga tcccgcccat tccgaaagca
    20941 tcaattttgc cgtccaaatc tctaatcaat tgcagcgctt tatcccagtc accatctgta
    21001 ccaatcctct caatacggaa cttctctccc atgaaatctg tttcaaaggc atggtcacgt
    21061 tttgatgatc ccagactgac actgacaaca tgtttcatac aaaaacctcc atcaaattca
    21121 tctggctgca cggatggatc ttaatacttc tttgatgatg gccggatcca gccatttgtc
    21181 ttccgtaatc ggagaagtcc cgattgcaat cgaaatgatc tgtccgttga ggactccctc
    21241 catcagcctg atcctttcat ctgaaccaat acagacaacc acatcatatt ggcgtacatc
    21301 agcaattaaa tccatgataa cattcagcag ttctttgccg gatcgatggt ttataaaatc
    21361 ctgtctttcc tgttccgtca tcagcatgat atattcaacg ccttcttttt ccaggatggc
    21421 cagaatctct tccttgttgc tgagcgggaa tccgatgcag gcgatgctct gtcctttacg
    21481 cagttctccc agggtttcca gacgggaaat aaaagtacgg tcaatcttga gtcgctccgc
    21541 cacctcctgc tgggaaaaac cgcgcgctct catctgtaaa acttttttca gggaagtttc
    21601 gatcttttgc cagctgatga ttttgtcctg gatacgaata aattccatga tggctcctcg
    21661 aacaccgttt ttgtgtacag ttttgtgtac agggctattt tatcatcctt tgcgttacaa
    21721 aacaagcaaa tgaagggatc gcggcaaggt gaaaagcatt gacattctgc gacggttgtt
    21781 gccggcgccg acaagattcg tttcctatac caaaaggcct tccaagatct tcatcatttc
    21841 ctgccgggtg acagtgtgat tgatttggtc gcgcagacga gccgcggcag gcattccttt
    21901 ggtataccag gagatatgtt tgcgcatttc ccgcacggcg atatgttcgc ctttgtagtg
    21961 aatcgccagc tcaagatggc ggatggccag ggttattttt tccttctgcg agggagcctg
    22021 gatcttttgc ccgtgttcca acaaggctat ggtttcacga atcaaaaacg gattacccag
    22081 tgcgccccgg ccaatcatga ccgcatcaca accggtttcc gctaccatcc gtaccgcatc
    22141 ctcggctttg ttgatatctc cgctgccgat gaccggcacc gtcgagtgac gttttacctc
    22201 agcaatggcc tgccaatctg cctgtcccga gaaaaattgc atgcgggtac gcggatgcag
    22261 ggtaatggcc cgaacccctt cggacactgc aatatctgcc atctgtaaat aattgcagcg
    22321 ctcatcatcc caacctttgc gcatttttac agtaaccggc acttggacgg ctgtcaccac
    22381 gctgcggatg atctttcggc tcagttccgg ttccaacatt aaggctgccc cttccccatt
    22441 gcgaacaatt ttgggagttg gacagcctat attgatatca atgatgtcag cccccatctt
    22501 ttcaaccatg tgcgctgcct gcgccatggc cccggggtcg gaaccgaata tctgtaccgc
    22561 caccggccct tcttcttcac cgggttctgc aatctgacgg ctcttgttgc tgttatatag
    22621 taaacccata tcgctgacca tctcagtaaa aaccagtccg catcccattg attgcaatat
    22681 cgtgcggaaa gcccggtcgg taatcccggc caaaggagcg gcgaccactc ggttttccag
    22741 ttttacactg cctatcttca acatcaccac accttaacca tgatttaact catacagcag
    22801 acgcagaccg tccagggtaa ggaattcgtc cacctcatca atggtatctg tttcgccagc
    22861 aatcagcgct gccaatcctc ccgtggcaat aacccgtaca tccccgccga tttctttttt
    22921 catgcgattg atgatgcctt caacctgccc gacgaaacca tacataattc cgacctgcat
    22981 gctcatgacc gtattcttgc caatcatgct tttggggatg ctcagctcca cccggggcag
    23041 tttggaagct cttgaaacca gggcatccgt cgaaatttta atgcccggag caatggcgcc
    23101 gcccagatac tcgcccgcct gactgaccac acaaaaggtg gtggcagtcc caaaatccac
    23161 aataatcaag gggcctttgt atttatggta agcggctacc gcattgacga cccggtcagc
    23221 acctacttcg cgcggattgt catatttaat ggccagtccg gtcttggtcc ccggacccac
    23281 cagtagcggc ttgcagccga aatagcgctg gcacatccat tccagttcca tattcaaact
    23341 gggaaccacc gaagatataa tggctgcctt tattttttct gcctgcaaat tttgataaag
    23401 aaacagcccg tgaatgatga tcccgtattc atctgccgta cgctgggtat cagtgcggat
    23461 gcgccaatgg tcaaccattt tttcctgatc gaatactccg actaccatat tggtattgcc
    23521 gacgtcaaat actaaaatca tacttttgcc tccacataag atctatttat tcacaaattg
    23581 gtgcaaggtt gtgcttttta cagttgatgc aaatcaaagc tgatatccag actgacagcg
    23641 gaatgggtaa gcgcacccat ggaaataaaa tccacgccgg tttgggcgat ttctgccagg
    23701 ttggctgctt taatgcctcc cgaagcttcc agcaaagccc ggccggctac cagcgtcacc
    23761 gcctccttca tcattgccgg aggcatattg tccagcatga tgatgtcagc gccggcttcc
    23821 agggcttcct gcaactggac cagattttcc acttcgacct cgatcttcag cgtatgcgga
    23881 accttggcgc gagccatgac aaccgcctgg gcaatgcctc cggcggcaat gatatgattg
    23941 tctttaatca tcacgccgtc ataaaggcca aagcgatgat tgcagccacc gccggcagcg
    24001 actgcatact tttccaatat ccgcaatcca ggcgtcgttt tgcgggtatc cagcagctgg
    24061 gccggatagt ctttgatcag ttgtgtcagg taatgggtct gggtggcaat accggacaat
    24121 cgctgcaaga agttcagggc agtccgctcg gcgctcagca aagccctgct ccggcccctt
    24181 actttggcaa tgatggaacc ggcactgacc ggacaaccat cttccaggaa acaggtgaaa
    24241 tcagcttccg gatcaagctg ggcaaaaacc gcccgggcta cgcccagtcc ggcaataatc
    24301 ccatccgctt tggcaatcaa aaagccttcg ctggattgct cttgcggtac cacactcatt
    24361 gtggttatat caccgtatcc catatcctct gccagactcc tgctgatcaa ttcatttaat
    24421 cccggataat ccattcgatt tcctcctgct cctggcctta attattcatc tgcttacaat
    24481 acttgcgcag tatgtatcat tagcaattga caaagctggt atgtttcagc cagcgtacat
    24541 catcacgctg cggaaaatca gagcgaaaat gccccccccg gctttctctg cgccacaggg
    24601 cagcttgtac gacgacatgg gcaaccgtca gcatattgac catttcaata aaagccaggt
    24661 tgtcatcccc ccgggccaga tggttataaa ggtattcgat ctcctggttg gccgctttaa
    24721 gagcttcttc attccgaatg ataccgactt tttcccacat cacatcctgc aatcgttgtc
    24781 tgatttcaac gggtccgatt tgcggatgca aaggggcata gactccaccc gctgcaaaac
    24841 ttcggcaaag ttcttccgct ttgacctgac gccgatacat gatttctcct gcccgctcga
    24901 caatgcgctg gccaaacaca atcccttcca gcagggagtt gctggccagc cgattggcac
    24961 catgcacacc ggtacaagcc gcttcgccgc aggcataaag tccgttcact ccggtttccc
    25021 cgtaagtgtt ggttttaatg cctcccatgg tataatgggc tgccggagat accggcactt
    25081 tatctttttc caggtcaatc ccgtgcagca ggcaagtgcg ataaatgttg ggaaaacgct
    25141 cctttactcc aggtatgatc gacatatcca gaaatacata ttcactgccg gtgagcacca
    25201 tctcatttaa aatcgcccgc gatacgacat cccgcggagc caattccaac agggaatgat
    25261 atttgttcat aaatctttcc ccgttggtat tatagagcaa tccgccttcc ccgcgaacgg
    25321 cttcggaaat taaaaatcgc tgcgtgtcat gactaaacaa aacggtggga tgaaactgaa
    25381 tgaattccaa atcactgagc tcacaaccgg cccgataagc agccgccata ccatctgcgg
    25441 tagccacatc agggttggtg gtatagcggt aaagctgccc ggctccgccg gtggcgatga
    25501 tcactgcccg ggcaatatat aaaatctgtc ggccgttttg cttgtcatag accagagctc
    25561 cctggcattc tttctcttcg cttcccaata aatcaatcag aaactgatat tcaaaaattc
    25621 tgatccgctg gtcggcttcg cagttcttta ccagggccgc acgaatcgtc tctccggtgg
    25681 tatccgcgga atgtaaaata cgggccttgc tgtgggcccc ttccctggtc agggatatac
    25741 ttccatcttt catatcaaag ctggcaccgg cagcaatcaa ttccctgacc cgctccgggc
    25801 cttcccggac cagcacatcg acggcttcaa tgtcgcaaat ccctgcgccg gcttccaaag
    25861 tatcctccaa atgcaaaaag ggagaatcct cctcgtgcac ggcggcagcg attcccccct
    25921 gagccaaccc ggtatttgaa tccttaatcg tctttttggt cagtaccacg acctcgccaa
    25981 aggcggaagc cttgagagcc gtaaataatc cggcaatacc gccgccaatg atcaaaaaat
    26041 ctgtatgcat aatcgttgtg tccggctgca gtttccaggt atatcttgta attctcatat
    26101 cgaacccttt attctatttg atttccagca tgttttgcag ggatcggaag gcttttatgc
    26161 gcacattttc tgttacttcg atctgttcct gcaatttctc cagggaaagc gccaacgctg
    26221 acaggctgat cattttcata tcctgacaga caaaatgact gcgggccaga taaaaagttt
    26281 tgtccggaca agccttgcgc aggctgtgca ggaagccctc ctcggtcccg ataataaact
    26341 ccttttctgc actggcctga acatattgca gtattcccgc agtggagcgc actgcatcag
    26401 ccaaggcaat cacttccggc ggacattcgg gatgaacaac gaccttggct tgcggatgca
    26461 ggctcatctg ttccttcaca tgaccggcac tgacaatgtc atgaaccgga cagtagcctg
    26521 gccagaaaat catctcccgt ccggtctgtg cagctacata tcggcccaga ttgcggtcag
    26581 gaataaaaat gatttccttt tcccggggaa tggaatttac caccgccagc gcattggatg
    26641 aagtgcagca aatatcactt tccgccttga ccgccgccga ggtattgaca tacgcaacca
    26701 cgaccgcctg gggatgctgg cgtttcatct ctcgcagtgc ttccggtgta accatgtctg
    26761 ccatcgggca tcccgcctgg gcaggcaaca gtaccatttt atcgggattt aaaattttgg
    26821 cgctttcggc cataaaattg accccgcagc tgacaatcac ttgcgctgag gttgcactgg
    26881 cctgctgagc cagctgcaga gaatcgccca caaaatctgc gatatcctga atttcaggat
    26941 tttgataata atgtgccatg ataacggcat tcagctcttg cttgcgccgc attatataat
    27001 ctattatgtc gttggccatg aaaatggcat cctcctacgc catgccgccc gggctgattt
    27061 tttgcacctc gttgtgctca ttcacataaa ccagcaccgg cttaaagcct tggcattcct
    27121 catcactcag gatggtatag gtaagaataa taaccacatc accacgctgt accttgcggg
    27181 ctgctgcacc attcagacaa acggtttttg agcctctttc accctcgatg acatacgtct
    27241 caaaacgttc cccattgtta ttgttgacaa tggtcacttt ctcattctca ataatattgg
    27301 ccgcatccat gagatcccga tccagggtaa tgctgcctac atagttgaga ttggcatccg
    27361 taactactgc acgatgaatt ttggacttca acatataccg gagcactttt tacacctcca
    27421 cgataagatt gtctatcaat ctggtattgc caaactttac agccagggcg atcagcaccc
    27481 ggccattgat ttcctctaca tcccccaaat cgtctgcagc ataaatctcc acataatcga
    27541 tcctggcctg gggggcggcc tcgatcattt ttctgatttc ctgcttgagg cgggcaacat
    27601 tatgttctcc attttccgcc aactgccggg ccttttgcag cgaacggctc aacaccaggg
    27661 cttgccggcg ctgctcatcg tccagatata cattccggga actcaaggcc agaccatccg
    27721 cttcccgtac gatgggcacc cgcacaatgg caaggggaaa attcaattcc tgtaccatct
    27781 tttcaataat catggcctgc tgcgcatcct tttgaccaaa gaatgccttg tccggctggc
    27841 agatattgaa aagcttgccg accaccgtcg tgacccctct gaaatggcct tctctggaag
    27901 ctccgcagag ttttgaggta atctctcctt ttaattccac ccagctgttg taaccttcgt
    27961 gatacatttc ccgcgctgat ggagcaaaaa tggcatccac tttttcctgg gccagctttt
    28021 gccggtcact ctcgatgtcc cgcggataac tgtcaaaatc ctcattgggc ccaaactggg
    28081 tagggtttac aaagatcgaa acaaccacga tatcacattg tcgtcccgct tcttttacca
    28141 aagacaaatg gccctcgtgg agatatccca tggtgggtac cagcccgatc tttctgcctt
    28201 gacgcttaaa ttccaggcac cagctttgca tttctttgat ctcattaaaa accagcatca
    28261 tgatccctcg tcgctgttgg atttttaatc cccaggaagg cctgtcggcc gtcccggagc
    28321 gcaaacctaa tacaatcttt ccagaacatc ttccttaatg gtaaaaccgt gttcttcccc
    28381 tggaaaagta cccgcttcca cttcctgctt gtaggcctgc aaggcttcct tgatcagcgg
    28441 ttccagattg gcaaactttt taacaaactt gggaatatgt cctttgaaca tgcccagcat
    28501 gtcgttgacc accagaactt gtccgtcaca atatgcaccg gcaccaatgc ctatggtagg
    28561 aatcgaaact gactcggtga tttttttggc aagcagggtg ggtacacatt ccaagacgac
    28621 ggaaaaagcc ccggcctctt cataggcttt ggccgccgca atcaagttct gggctgattc
    28681 gagatctttg ccttgtactt taaaaccgcc aaaagcattg actgattgag gggtcaagcc
    28741 aatatgtgcc atgactggaa tttgcgcatc caaaatggct ttggtgaagt caacccgttc
    28801 cagtccgcct tccaacttga ctgcctgagc gccgccttcc tgaatgagcc gtccggcatt
    28861 gcgcactgct tcttcccgtg aaatatggta ggacaaaaac ggcatgtcag ctaccaccaa
    28921 ggcatttttt gttcctctga cgacggccgc agtatggtgc accatgtcat ccatactgac
    28981 cgccagggta ttctcgtatc caagctgcac catgcccaat gaatccccta ccagaatcat
    29041 gtctatgccc gcttcatcca ccatggctgc cattgaataa tcataggacg taagcatcgt
    29101 aatactttcg ttttttgctt ttttatcttt aatcgttgct attgttactt tgctcactgg
    29161 cgcacctcct tactgactga gctgattcga gccagctctt gcataaatac ggtctgaaac
    29221 ttttccatgg cctctttgtc gatactgcct ttttccaggg caatttgcgc agtataatag
    29281 cccaaccatc cgtagagctt ggttaattcg ggtgccattt tttcaaggca atccaaatgt
    29341 ttgtttattg ttgatagatc accccgggcg attggcccgg tcagggattt gggaatacct
    29401 atattttcta cattgtgcag tgttccgtta attaagggca gcaaagcacg ggtagccatg
    29461 cttttgggaa taccggttga ttccagcagt ttaaccccaa aatcaattat tgttaccaga
    29521 taattggata ccacacaggc accggcatga tataaaggct tggccttgcg gtcgataaag
    29581 aaatattctc ctcccagcgt ttccacaata cagacagcca catcaaaagc ctgcctgtcc
    29641 ccttcgatgc taaagacaga cccgggaaga ttcttaatcg ccatatccgg gctggcaaaa
    29701 gactgaatgg gatgcacgga aagaacccgt gccccaaact ctttggcccg gtccaatatc
    29761 tccgaggatt gtgccccact catgtgaacc agggtgtgtc cgctatgaat ggccctgcga
    29821 tcagccagcg tatccacaac cttctgaatg gcactgtcac acggagtaat aaaaagtaaa
    29881 tcagctgcgc gagcaacgtc ttccgggttt gccgcaaccg tacaaccggt ccgttttgcc
    29941 agcagctctg tagattcagg tttaatgtcg aatgcccctg taatctcata gcccttgtca
    30001 tgcaaaacca caccgatggc cgttccaaca acccccgccc caataatacc gatctttttt
    30061 ctcaacatct aatgaaaccc cctcagaaaa caggcacaca aaagcactgg cctgttttgc
    30121 gtattccgtc ctggtccgtc aaggatccag gcttcttatt caggagtact ggcgggagac
    30181 tccctatata attatttccg ttcttctgat gctttggctc gcatcctcca gctgtgctgc
    30241 cctggccaac acctccccga tccctttgcc atccggaaat accagcaacg gtgcgatttc
    30301 ctgcaacggg actaaaacaa acaaacgagc atacatgtgc ggatgaggaa ccaccagctc
    30361 cggtaaagca atggtttcct ctccaaataa aaggatatcc agatctatta cccggggacc
    30421 ccatttttcg ttcctgaggc gtcccaattt aatttcaatt tgttgaacaa catccagcaa
    30481 ctccagcgga gtcaattccg tatctatttc aacgacctga ttcaaaaatt catcctggtc
    30541 ggtctttccc catggtgcgg ttatatataa agacgactgg cgagtcacct tgatcttgtc
    30601 atgatgggcc agcaactcaa ccgcctgcgc cagattgact gccttgtttc ccagattgga
    30661 accgaggcca agaaatactt tcccattcaa acagtgttca ccctctaaat tagcatatag
    30721 gttttccctc tgcaagagtt aatttcggga tctggcaatc gaaaccccaa aataatcaaa
    30781 ctctccctcc accggagcct ccggcttgta aacagtaatt tctacgccct caacgggaaa
    30841 ctgctccagc acagccgcgg caatcttttc cgccattgct tccaacaatt gataactttc
    30901 tttttctaca atatttttaa ccagagcgaa aacgtcggca taagacacgg tatgacttat
    30961 atcatcacga tgaccggcca gcctggtatc caaaaacaaa tctgcatcca ccttaaacgt
    31021 ctgcggctgt gtcttttcaa agtccaatac cccgtggcaa cctttaaaag tcatgccccg
    31081 cgccatgatc ttatccatgt tcgctcctta ttgcatccag catccgtgcc atccggcagc
    31141 tctcctgcac atcatgaacc cgtacaatgt ctgcaccgtt cataatcgcg gcagccatca
    31201 cagccagact tccttccagc ctttcttcca cttccaaacc gagtgttttt ccaataaatg
    31261 atttgcggga agcaccgatc aacaaaggcc agcccaaact cttgaaatct ccgagtcttt
    31321 taatgatcag gcggttttgg gccggcgatt tgccaaaacc caagcccgga tcaagaataa
    31381 tctttcccgg tcccaaaccg gcttcttccg cctgcccggc agattgcgcc agttctgcag
    31441 caatatcgtc aattaaattg acatacggtt catgtcttct caattgcagt cgattgtgca
    31501 tcaaaacaac cggcgcctgc cacttgatca aaacatccaa aagttctgaa tccatttgca
    31561 gactgccgat atcatttatt aagtgggccc cattctccaa tgattgccgg gcaacttccg
    31621 cccgaaaagt atccacagaa acgagcatgc cggcatcaac cagtttcttt accagcggaa
    31681 tcacgcgcgc cagctcttct gcctcactga ccatgatgga atctggacgt gatgatgctc
    31741 cgccgatgtc aatgatgtca gcaccttcgg cttgcatcgt cgaagcccgg gcataagcct
    31801 tgtcaggctc caaaaaccgc cctccgtcac taaaagaatc cggtgtaata ttaaggattc
    31861 ccatgatgac cgtcttgctg cccaatgcca aattttttcc ctgtgccaaa gccaaatcgc
    31921 tgagcggcgc ttccatggtt tgcagtatat tttctatctc tgccgccact gtccgcaggc
    31981 caaagggttg ttgtttgagt ttttccaaca gaagtgtata gtgtttcaag gtccccagca
    32041 acagcacatc gccaaaacct ttttcgcccc cggctgcatc ccgggtaacc gctgcttcgc
    32101 cgcccttggc caacatttcc tgctttaaaa tattggccgc ccggtgggaa atatgcttta
    32161 gtttgatgca tcggaacacc gccttgtcga ccatgtaagc aatacctgga gaactcacgc
    32221 caatctcttc cagatattgc ttcgcttctg cccggttttc aattaatact gcatgatgga
    32281 ccaatatctc acctcaacat tagtaggcaa acgtggtaac actgaactcc ttgttacccc
    32341 gttttggaca ggtgttgcgt tcttcacact gactgcagac ccgggacaaa ttgtcagccc
    32401 gatggatcct ttcacccaga aaactcatgt cccggctgat gttgcgatgt tcatatatcg
    32461 cgcggcaaat gaggctggtt tcattggcat taaaaaaggc acgcggtaat atctcacgtg
    32521 ccagttttgc accgaaagaa gcatggtcga ctccttcccg gtattctttc catttgccga
    32581 tatcatgcaa caagccaccc gcatagatac attccttggc ggccagtttt ccgcttaccc
    32641 cgctctctct tacaaaataa ttgagatcgt tatgctccaa gaccaatata taagcaatcc
    32701 gggcaacatc aatatgatgc tgcagaccat gctgacagta tgcggtcaca ttctcatcat
    32761 ctttattgcg tttcatatag tcaacgtatt gttcatcctg taaaagcaac tctatccgct
    32821 tcaccatcgt caccccgtga attattttat caaccggctt acgtatatat taaacaaaga
    32881 agcgataaat cctgctgtac aaggcatatt ttatgggagc gtttcctcag ttgcgggcgc
    32941 cgactctgtt tgttgctgtt cgtcgttcat aatccgttca aattcatccg agtcaatcgt
    33001 ttccgcctcg atcaaacgcg cagcgatatg gtgcaattta tcgatattgt cacgcaaaat
    33061 cttttctgcc ttgttgtagc tggtgtccat ataatgagac gcttctttgt caatggcata
    33121 agcaatcgca tccgaataat tgcgatcccg ggaaatgtca cggcctaaaa acacttcttc
    33181 actccggtgt ccaaaggtta acggtcccaa atcttcgctc attccatatt cggtgatcat
    33241 tttgcgaaca atgctgctgg ctctttccaa atcattttga gcgccggtgc tgatatcatt
    33301 tagaaccagc gcttctgcga cccgaccgcc caacaatgtg gtcacctcat caagcatata
    33361 tgatttggtg atataattac ggtcttcctc tggcaaaagc agggtatacc ccccggcccg
    33421 tccgcggggt atgatcgata ttttatgcag tttgtcggta tgcggcaaga aaaatgaaac
    33481 caaggcatgg cccgcttcgt gataagccac cagtttcttt tctttttcgg aaatgacgcg
    33541 ggtcttcttc tccggaccgg caatcacgcg ctcgatggat tcctccaatt cttccattcc
    33601 taccgaatct ttgtcacgcc gggccgacaa cagggctgcc tcattgacca gattggccaa
    33661 atccgccccg gtaaaaccag gcgtgcgttt ggccagtatt tcaagattca cattttcatc
    33721 cagtggttta tccttgacat ggactttcag gatggcttcg cggcctttga catcaggacg
    33781 atcaatcagg atatgccgat caaagcgtcc tggtcttaac aaagccggat caagaatatc
    33841 cggtcgattg gtggaagcca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33961 nctgatggaa aagaagggta cgccggcttc cccggcaacc gccttggcca tcagggtttt
    34021 gccggtaccg ggagcgccga acaataaaac cccccggggg atcttggctc cgattttaat
    34081 aaacttctcg ggattcttca gaaactcaac tacttcggcc atttcctctt tggcttcctc
    34141 tgcaccggcg acatcagcga aagtggtttt gacctgttcc cccgacatca tacgcgcctt
    34201 gctcttacca aactgcatga ccttgcctcc gcccccctgg gtttggttca tgataaacaa
    34261 caggaagacg atcagaatca caatgggaaa gagtgtactg agcaacgtcg tccaccaggg
    34321 tgccggcggt acgggttggg ctttaacctg tacgcctttt tcctgcatga ttttatcgat
    34381 gttgctttcc ttggtagcta cggttgtcac cttgtcccca tttttcaagg ttgccgaaat
    34441 cttgtacacc agatcatcaa tcacgatatc agcattcttt acttgacctg cttcaacctg
    34501 gctgtagaat tgtgaatacg tcaattcccg aacctcggtt tgcggggtgg aggtcatttt
    34561 gattgcaaat aatgccagca atacgatcaa tgtatatatc gctatgtttt ttagagcctt
    34621 gtccaattaa ttacctcccc ggtttctcac aaacatttct gttatatgga attttctaga
    34681 cgaattaata ttctgaatca aaatatttta tcatatggtt atctgctttt caaatcgggc
    34741 tgccgccttc ttcagcgctg atcagcagta tgtttttgct ttgttggttt accgccattg
    34801 ttggacagaa atataaccct ggcatcgcat atatttctcc gccccgggag gcaaggatgg
    34861 caatctgatt tctttcccgg actggaattc tctgctcgtt caaatacttt ttaagttttt
    34921 tatgcccggc agatccggtc agtacgatca tatctccggg gcgacgggag cgaagcacca
    34981 atggcaaaga aagtttgtca tagtccagac aaatatcacc cggtgctgct ttgaaccccg
    35041 cgctcgtttg cagcgcaaaa tgatatctgt tcccattggg caagacaacc tgcccgggaa
    35101 tcccaacctc tatacagaaa ctcccggtct cttgccactg actcgtaaat actattctat
    35161 cataagactt gttaatgatg attccctttt ttaactgtag tatttttgca gaacccggct
    35221 tgtgcagcaa a
//
LOCUS       DAOTHZ010000014        35041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1347_length_35041_cov_7.959584, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000014 DAOTHZ010000000
VERSION     DAOTHZ010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 35041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35041
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1347_length_35041_cov_7.959584"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..600
                     /locus_tag="P5549_03075"
     CDS             7..600
                     /locus_tag="P5549_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRW12087.1"
                     /translation="MSLDHINAQQALQRLKEGNERFRSNLQSKDCCSQRRQELLTKGQ
                     TPFAVILGCSDSRIPPEVIFDQALGDIFVIRVAGNVVSAIELGSIEYGVEHLHTPLLV
                     ILGHDNCGAVKATVDGGEAPGSIGAIVDMIKPSVEKAKAAGASGDDLYEKACEENLWA
                     MLAIVKQSPIIEEMVAHGHLEIVLAKYLQNSGEVVFF"
     gene            823..1077
                     /locus_tag="P5549_03080"
     CDS             823..1077
                     /locus_tag="P5549_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12088.1"
                     /translation="MLKYHFEILKTTPLKGHKLQGTKDYETHVTIEKDDKLIFDEVVQ
                     VRKNKEGVFPDLSMANKKIAAASIRRELAEKLKEFFKRMK"
     gene            1260..1796
                     /locus_tag="P5549_03085"
     CDS             1260..1796
                     /locus_tag="P5549_03085"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRW12089.1"
                     /translation="MNNYFTVDYIILGCLSIEPMSGYDIKRLISISTSLFYNASYGSI
                     YPTLKKMEEAGLVVAEEAVENGRFKKIYALTAEGHKEFMAWLEEPPKPIVIKNDMLLR
                     LFFYRQLPREKVLSITAAHIEQLKAFKLELDEIEATEGSWADKFQYDTLRWGKDFYDF
                     QISWYEKLLYQLENETSQ"
     gene            1927..2895
                     /locus_tag="P5549_03090"
     CDS             1927..2895
                     /locus_tag="P5549_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRW12090.1"
                     /translation="MKTRITEMLGIKHPIICGGMLWLCKPDLCAAISNAGGLGNITAG
                     NYDTGEELRAALKQTKALTDKPFGVNVTLLPSFRITQETYDDYFRVCCEEKVAAIEVS
                     GAPAKRYLDQVHEAGIKIFHKVGSLRHALNIEKLGYDGIFAAGIEEGGHPLNDDVTTM
                     VLTPRIAESVKIPVVTTGGIADGRGLAAALALGAEGVMMATRFINTKECIVHENIKQE
                     IINRQEQDTTLICKTLNLQGRALKNELTQQVLDIEAEGKDLGRIFPLITGQRVKEAWN
                     TGDVNGAAFMVGQSIGLIQDVLSCQELMDGMVKDAEAILRKNLERF"
     gene            2972..4474
                     /locus_tag="P5549_03095"
     CDS             2972..4474
                     /locus_tag="P5549_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRW12091.1"
                     /translation="MSEYDVVVIGAGNGGLTAAASLAQKGMKVLMLERHNVPGGCATS
                     FMRGRFEFEVALHQLSGFGTKDKPGPLRMFLDGLGVVDQLDWVAMENLYRVVLPGELD
                     LILSSDREKMIATLQESFPQDSQAIAEFFDLVYKFFHEIIRAFFMKDPEVSKEKYPLY
                     FKYSLKSTQEVMDEFFTDPLLQAAISVYWCYVGLGPNQLTFSDFAALMFGYLEFKPFH
                     LKGGSQALSNAILDQFLNHGGEVRFNCAAQRILVENGHVKGVATEDGDVIDCKYVVSN
                     ASSVETYVDLMEPSPAADQEIQKMAGSTVGPSSLTLYIGLDCSPEEVGINEATNFILT
                     SADMNGGYERFKTLDTSDDMLLLTCYNLLDPSFAPAGCSQIVIVGLKYADPWIEVPPA
                     EYYQIKYQAAEGFLQSAEKVFPGLREHIEEIEVATPITHMRFLGQPGGAIYGFDKFAK
                     ENSLFTNRKSPIKGLYQAGAWAGSGGFQPTLTSGGQAARAIVKDFRGVEK"
     gene            4471..>4994
                     /locus_tag="P5549_03100"
     CDS             4471..>4994
                     /locus_tag="P5549_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRW12092.1"
                     /translation="MRDFREDIEGYGEIKQEMEISRKYGINYRVQKNRRADVVSRLHP
                     KVLKLRLADIILETPTTKTLRLVAREGNLPPFQAGQYINLQVEINGIRTSRPFSISSS
                     PRQTAYYDITVRKIENGFVSAYLFDDVRVGDEFTSSGPAGNFYYNPLFHGQKLVFLAG
                     GSGVTPFMSMIREN"
     assembly_gap    4995..5094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5095..5442
                     /locus_tag="P5549_03105"
     CDS             <5095..5442
                     /locus_tag="P5549_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HRW12093.1"
                     /translation="PARKIRHEMFGNPADITAQPGWPREIRKEAAFTVKVEGHKSLAA
                     QAGEPLLIALEKAGLIVPSLCRSGECSLCRVQLLSGKVFQPQGVLMRESDKKYGYIHS
                     CKAYPLEDLVIRL"
     gene            5501..5623
                     /locus_tag="P5549_03110"
     CDS             5501..5623
                     /locus_tag="P5549_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12094.1"
                     /translation="MDLLKIYKQGEDDPNAAAFYLENPQAVLMDFSGKFEKINM"
     gene            5650..7890
                     /locus_tag="P5549_03115"
     CDS             5650..7890
                     /locus_tag="P5549_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRW12095.1"
                     /translation="MGEWKKTSCVLCAQSCGLEMLIEDNRIIKVRGAKDNQRSQGYCC
                     RKGLNMAHHVHNADRLKYPLKKVGDHHERISWEQALTEIAEKLLSIKEKHGGKAIAYM
                     GGGALGGQMQVGVGLRMLGFLGSRNYYSSITQEFSNSFWVDGRVTGKQSIVTMPDAHH
                     ADTLVAWGWNGWMSHQEPRTRELILEFKKDPDKKLIVIDPRRSETAERADIHLPIRPG
                     TDSMLLKAMIRIILDNSWEDQAFIAAHVNGWEEVKPLFDGFDARRAVTEVCGLDYETV
                     LEVTRLLAQTRSCIHQDLGIYMNRNSSINNYMLHILRAITGRFCVEGGQIIPAFLFPM
                     GSNSDERNPKVWRTVKTNMFPILGSFPPAVFPEEVLNDHPDRIRALIVSASNPLRSYP
                     DTQAYEKAFAEMELSVCLEIVYNETARCSDYVLPCLSYMESYDTVCFNYSYPDLYFHM
                     KQPVLEPLSSESREGSRIIMDLIKAMGFLPEFPQSLYDAGKEGISAYLGAMGAYVQEN
                     PSQAKLIPLILAETLGTALGSVNQAMIVGLLVSSSKEFKAGAVAMGYPADFSMIEAMY
                     KDILAHPQGLILSRFQGDNFNMLKTEDKKLNLVIEELVDQMQAASIENEIEKLKMPAE
                     FPLILHSGLHHETVANTSLRNPEWNKDKRCMTMLMNEVDAARLDLADGDKAIITTKAG
                     SAEVEVEISSRAAVNCVYVRHGQGLIYDGVKYGVNVNDLVLTTDRDEMCTPMHRRIPC
                     RVEKAS"
     gene            complement(7972..9018)
                     /locus_tag="P5549_03120"
     CDS             complement(7972..9018)
                     /locus_tag="P5549_03120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRW12096.1"
                     /translation="MHTIISDRPRKILMISPYRHSQRGNSITSMRLKTGLEKQGFMID
                     WITMEESDVLPQLQQQLDSGCYGLIHAFHALHWGRLLDQLPQIQQIPVLLTTTGTDLH
                     FDLSGKDSPVLARSLFSAQKIVLFHQDFTTQIAVPFPSLAARLVVIPQGIFFENVSRE
                     TFLPLDFKQDDFIFLFPSGLRPVKNFELTLNALENIQPDYPQLKLVIIGALIDPDYSR
                     RMLQRIDALPWVTYMGEIPHASVRELILHADVVVNSSHAEGQPQGALEAMSLGLPCIL
                     SAVPGNLHIIEEGREGFYVHDEKEFARAARIFMDHPRRTKMMGQAARKLVEEKFSLEK
                     ELNAYAALYQQLCQ"
     gene            complement(9041..9700)
                     /locus_tag="P5549_03125"
     CDS             complement(9041..9700)
                     /locus_tag="P5549_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRW12097.1"
                     /translation="MEKLLIFAPHPDDDIIGCGGTIARHMAHGDEAAIVYLTSGENGS
                     LSYNPSELAALREEEASRAAAMIGVSDVIFLRQPDGYIVWSKELMDTLVTVIRNQQPS
                     MVFLPHGEEAVRDHRQSCELILEACQRAAGPWFPACGKPWGGGKIIAYEVWTPLSRYN
                     LTVDISEYMQKKLAALGEHKSQLADIAYAEAVQGLNRYRAVTSGTGQFAECFQLIKTR
                     S"
     gene            9904..10689
                     /locus_tag="P5549_03130"
     CDS             9904..10689
                     /locus_tag="P5549_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12098.1"
                     /translation="MQWETVNLEIMDSGAAIMTFNRPDFLNSVNEQFCKDVNAACKEA
                     ADNNDIKVLIVTGAGKGWSSGGDVSTLYSMTTPLNAKETYDASASVVTAIYELQKPVI
                     AAVNGVVAGASTATCLACDLIIASEKARFGFTFMQLAFCPDSGASYFLTQKVGYHKAA
                     EIIWFGKILNAAEANELGLVNTVVAHDELMKTAVEWAEKLTRLPLVTVGLDKKLLRAS
                     LKNDYYQQCELEALNQVLCWSSEDFKEGCQAFVEKRKPNFKGK"
     regulatory      complement(10881..11000)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     regulatory      11206..11324
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11425..11871
                     /gene="nuoE"
                     /locus_tag="P5549_03135"
     CDS             11425..11871
                     /gene="nuoE"
                     /locus_tag="P5549_03135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRW12099.1"
                     /translation="MSRIQEIIAQYGDKQGGIIEAYHAVQKEFNYLPEEAVIEAASVF
                     GVSEAQAYGVATFYSYLSVKPRGKYIIRMCESAPCHVAGADEVIQAIEKELGIKVGET
                     TADGKFTLELTECIGQCQATPVITVNSEPIFDVTPQKVPEILSALK"
     gene            11930..13144
                     /locus_tag="P5549_03140"
     CDS             11930..13144
                     /locus_tag="P5549_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRW12100.1"
                     /translation="MAKEMRVLLEYADRIDPASAEDYIKVGGYKGLEKARKMNPRDLI
                     QEVKDSKLRGRGGAGFNAGMKWSFVPQNVPIKYVVCNLDEGEPGTYKDRIICEKNAQA
                     LLEGMAICGHAIGSNQGYIYCRGEYPFVVDLLRKAIDSASEVLGDFNIEVRMGAGAYV
                     CGEETALIESIEGHRGEPRFKPPFPGVEGLWQVPTVVNNVETYACLPYILVNGAQWFA
                     GIGSSSYPGTKVLSLTGDVINRTYFEVPTDMTLREVIFELGGGIKNGKKFKAVQVGGT
                     SGAFIPEHYLDTKIDFDSMGAIGAALGSGAVFVLDESRDILDVATRISKFFEHESCGK
                     CNPCREGTFRSREILERLNAGKGSEADLDKLLLLSKVMQKACLCGLGQAAPIPTESTI
                     KHFRHEYLRKVQ"
     gene            13169..15871
                     /gene="fdhF"
                     /locus_tag="P5549_03145"
     CDS             13169..15871
                     /gene="fdhF"
                     /locus_tag="P5549_03145"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:14228..14230,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRW12101.1"
                     /translation="MISLTIDGMEVQVPRGTMLLDACRQAGADIPTFCYDSDLKLAGS
                     CRMCVVECKGRRNLLAACVTPAENGMVINTESADVVETRKVILDLLLARHKLECHVCE
                     KDGDCKLQDYCYRYGVKESSLGEEGPHYYKEDANPFIERDYDKCIMCTRCVRACEEIT
                     GACAINLEDRGHHVHIATAFNGLLEDSPCVFCGQCIMVCPVGALTSKISAGKGKPYQV
                     KKVPTTCTYCGTGCTFELNVKDDEIVGVTSMRNYQQSPVNNGALCVKGRFGWDFVNSQ
                     ERLTTPLIRENGELRPASWEEALDFAAGKIKEVKEKHGPDALAMFSSARMTNEENYLA
                     QKLMRAVIGTNNVDHCARLUHSVTVAGLGAAFGSGAMTNSINELQNDAKAIFLIGTNT
                     TENHPVIGYKIKSNVRKNGAKLIVADPRRIELAEMADVYMQFRPGTDVALVNAMMNVI
                     ISEDLLDKEFIANRTEGYEAMAAVLASYTPEAAEKITGVPAADIRKAARIYAEAEVAS
                     ICYAMGLTQHSTGTDNVKTVCNLALLTGNLGKPGGGVNPLRGQNNVQGACDMGALPVC
                     YPAYQAVTNPDIREKFEKAWGVPLSPSNGVTLTKAINMAHHGEIKSIIVIGENPMVSD
                     PDLHHVEEALENLDFLMVQDIFLTETAQKADVVLPGVTFAEKDGTFSNTERRVQRVRQ
                     AISPRGDARQDWQILCELSTRLGYPMYYASPEEIFNEMRTVTPSYAGITYERIEECGL
                     HWPCPTEDHPGTPILHKEKFNRGLGLFHAIDFKEAAELPDAEYPILLTTGRSLFHYHT
                     GTMTRRSVGLDAIQPENELQIHPLKAAELGIHDGEKIRVTSRRGSIELKARVTDIVDE
                     NVVFTFFHFAEAAANRLTNAAPESLDPVAGIPEYKVSAVKIEKI"
     gene            16130..16327
                     /locus_tag="P5549_03150"
     CDS             16130..16327
                     /locus_tag="P5549_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW12102.1"
                     /translation="MFINNTCNGCGICVDLCLGKAIYLADDKAVINYEKCLGCGICVN
                     FCDLGAIREDWEKMFYGMGAE"
     gene            complement(16340..16474)
                     /locus_tag="P5549_03155"
     CDS             complement(16340..16474)
                     /locus_tag="P5549_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12103.1"
                     /translation="MKTQVAIIAVVVEETESAEKLNRILHQFGQYIIGRMGLPYQKKI
                     "
     gene            16872..18470
                     /locus_tag="P5549_03160"
     CDS             16872..18470
                     /locus_tag="P5549_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRW12104.1"
                     /translation="MSLINAGKLYPALDSIPGEFCPPVFKPDARYLVNGRIITAASRE
                     VFSPIQFHSGQGVQPYCLGVTPWLEPDEAMEALLAAETAYDAGLGEWPQMPVSERILC
                     VEELLQRMRPLEEEFVLRAMWEIGKSYQACKDEFKRTIKYAEDTVARLRQDDRNSSLV
                     QQLDGFEAQIRRCPLGPTLLMGPFNYPLNESFAMLVPALVMGNTVVMKLPAWGAWALE
                     PFLDALCESFPPGVVNIINGDGAAIITPIIRRGTVNILGFIGSTRVAHSIVGQHPHIN
                     RMRTILGLEAKNPAFIFPDCDLELTVRECLQGALEYNGQRCTALKHLWVHKDILDRFL
                     TALNEAVGQVKCGLPWEKEVMITPLAEKDKCARLSDWIDQAYSHGARIVNPGGGEHAG
                     NLFYPAVLYPVTSNMDLFHVEQFGPIIPVSCFAALDEIAAYMAQCPYGQQASIFTRSP
                     ALAAPLIDILVTQVSRINLNAQCRRGPDDLPFTGRKDSAEGTLSVEDALRSFSIRALV
                     VANQSGQELFEAILDKGNSKFLRR"
     gene            18616..21234
                     /locus_tag="P5549_03165"
     CDS             18616..21234
                     /locus_tag="P5549_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW12105.1"
                     /translation="MGALKIKENVYSVGVQNPDLRVFDIVMRTEYGTSYNAYLVKGSD
                     KTCLIETVKDRFFDEYLGNLQEVVKLEEIDYLIMNHTEPDHSGSVKKLMDKIPGLTIL
                     ASPTALKFLKEITNSKLNAREVNHGEELDLGGKTLQFISAPFLHWPDTMYTYLQQDKV
                     LFSCDSFGCHYSDPVMFNDLIDKDFTDAYKYYFDMIMGPFKPYVLEALEKIKDLEIDV
                     VCPGHGPILRQNIEYYFDLYRQWATPPASDGDERPKIVIAYVTAYGYTETISNSIVEG
                     LEMIAEFNVMKFNLVEDPVSAVLEALPGADGLLIGSPTINGDTLPPIWELLTHLSPIV
                     HADLVAAAFGAYGWSGEAVPNIEARLNALRMKVLSGLRINFKPSEANLEEAFTFGMDF
                     AKAILEKRQPQSQKKWRCLVCGYIGEGPEPPSVCPACGVGAENFVEVRMEDEFRNDTN
                     DTFLIIGGGIAALSAAAAIRKRNETAAITILTEESVRPYYRPALSDYLSEELEDKKFY
                     VYSDDWYEEKRVEVKTSCQVTAIDTKARTVTVADGRSITYNKLIIATGARSNIPPIPG
                     AEKPGVYALRSYEDAQILKKAIQSAKRAVVIGGGVLGLEAVWEMISQGVDVSVIENNP
                     RIMPRQLDEGASERLQKLMTERGVKLYMGIGTEEIGGEGQASGVKLKNGQWLPADLVL
                     LSTGVKPNVELAQAAGINVDRGIVVDTGMRTSASDVFAAGDVAQVVDRLIGLWSISTE
                     MGNIAGANAAGDWLDYKEPTISTMLAAFDHEIFSIGEVNLPPEECRVIEVWDPIEDFY
                     KKTYIQDGVMMGEIIIAPQVNTTEAFRSIGRDGSGKKRVTKWKCLVCGYIHEGPEPPD
                     ECPVCGAPKDKFEPIG"
     gene            21340..22119
                     /locus_tag="P5549_03170"
     CDS             21340..22119
                     /locus_tag="P5549_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRW12106.1"
                     /translation="MLIGVLAGCLGALVGLGGGVIMLPGLQLLAGFDLTKAIGTTLFA
                     VIFTSISAAQGHYRAGHVHMRSAGYAALGGLVGVLLGSYAFAQYLCNGFSVLETMLGL
                     FFLFTAYRTTKDTWLDIRRRGKNATDADAGEKARFASLMALGFITGSLTGILGIGGGF
                     ILTPGFMFLAGMSPQMAVGTTMLTMLPLSLSGGLIKLYQGYVNLPAGIVLGLGTALGA
                     QAGVWLSTRMSAILLKALLSIVFFILAADYLLPLLQRQLLP"
     gene            22147..22806
                     /locus_tag="P5549_03175"
     CDS             22147..22806
                     /locus_tag="P5549_03175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRW12107.1"
                     /translation="MIPLRDSTPSDRFPLVTVVLILINLLIFMFETTLGSAALEALVY
                     TYGLVPSQMIHYNGPLTGYLPFISSTFLHGSWMHVIGNMWVLWLFGDNVEDKMGHGRF
                     LFFYLLCGLVAGITHVAFEPGSQVPVVGASGAAAGIMGAYFLMFRHARVFTYIPPIFL
                     FNIPAGIYLGFWALTQLFYGTFQTLAQGQIAFWAHVGGFAAGMIIYRFFLQARNEITE
                     V"
     gene            complement(22879..24039)
                     /locus_tag="P5549_03180"
     CDS             complement(22879..24039)
                     /locus_tag="P5549_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12108.1"
                     /translation="MLSTFLSKEDCHFLDLSVFINCSPEKILYYYYNSFIRIHLETYH
                     QMKEWSQGEDSPKSCLNQWMELLETELNSREDLAILQENEYLHEIGPYYYGPTNTRIY
                     FFKLSTMSNEPLTSVDFAVLFNLNKTPALDKRLQKYYKSRKTIKKSPPSREDLVTDLD
                     MCLQSLQQIERINRHCDYWQVFLNQRNDLLLQQDLLPPEPEALIAKPAKPEEPQLSFS
                     SLLAINHSKKRHREYVQSCGEYNRRMKIYLIRYREHEKACERYKIALQQWEENHLLLA
                     EKCIEQIEEATAKLKTAQGLLEMYQFILDKCYLHKDYHQTNALSTCKHYLLTGRANDL
                     QDCINLYEEERHWREIKASQERIENTIYFLQADTDAPVLANPASLIASTMDL"
     gene            24220..24936
                     /locus_tag="P5549_03185"
     CDS             24220..24936
                     /locus_tag="P5549_03185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRW12109.1"
                     /translation="MYSAILFDLDGTLLHIDMEEFLQYYFAEMMRAAREAGYKEGQKL
                     VEQVFASTSLMLNDFSPTVSNEAVFMGDFLSHCDYPEEEVRTFFDHFYQHHFPRLKVH
                     CRPFPGIPGMMQELFQRGLKVVIATNPVFPREAIQQRLQWAGIDDLPYALVTSYEEMH
                     FCKPQLAYYEEICQRIGVGADECLMVGNDVDEDLPAGLLGMKTYLVEDLLIDKGQSRL
                     FPDWRGSVNDMIAFLKKTGS"
     gene            24996..25190
                     /locus_tag="P5549_03190"
     CDS             24996..25190
                     /locus_tag="P5549_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HRW12110.1"
                     /translation="MSRKKPKANRPLTERDLLKLEIAREMGVLEQVEKEGWQSISNAT
                     CGRLGAIMRQRLKLTDRNTE"
     gene            25302..25766
                     /locus_tag="P5549_03195"
     CDS             25302..25766
                     /locus_tag="P5549_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRW12111.1"
                     /translation="MNEIERVRSYLQEKNPRLQIIELDQNTSTAPLAAAALGLEVGQI
                     AKSILFKTKSEQYLMVVSAGDVKMDSKAIKETVGERVRMANPEEVYHVTGFSVGGVCP
                     FALAHEVPIYLDESLLRYDVVYAAAGTANTALPITFTELQAITAGRPCKVSL"
     gene            25801..29196
                     /locus_tag="P5549_03200"
     CDS             25801..29196
                     /locus_tag="P5549_03200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRW12112.1"
                     /translation="MSKGFVHLHNHSEYSLLDGACRVKELVSQAVQLGMPAVAITDHG
                     VLYGVIDFYREAKKQGIKPIIGCEVYVAPRSRLDKEVRIDDNQYHLILLCKNETGYKN
                     LVKMVSRAFIEGFYYKPRVDRELLEQHHEGLIALSACVAGEIPQMILTGQGETARETA
                     LWYQQIFGAGNFYLEMQDHGMDAEKIVCRELARISQSTGIPLVATNDLHYIRQEDAAI
                     HDVLLCIQTGKTVQDEERMRFPGNDFYMKSEAEMRSLFADFPQALDNSLLIAEACNLE
                     FHFGEFHLPYFDIPAGYDPETYLEQLAWEKFAQRYPQPTEKIIERFNYELQMIKQMGF
                     AEYFLIVQDLVNWARYNQVPVGPGRGSAAGSLVSYALGITNIDPLKYDLLFERFLNPE
                     RVSMPDIDIDFCFEKRDQVINYIVERYGADRVAQIITFGTMAARAAIRDVGRALNVPY
                     GEVDKIAKLIPAELGVTLDRSLEIAPDLIELYQNNYDARKIIDLARAIEGMPRHASIH
                     AAGVVIGKEDLSSIVPLQKTADGHVITQFAKETVEDIGLLKMDILGLRTLTVIDRALA
                     IIKKTRNLDISIDELPLDALEVYKLLQEKNTIGVFQLESDGLRRILGEMQPSRFEDLI
                     AIIALYRPGPLGSGMVEDFINCKHGRQKIEYIDPRLQDILQETYGVVLYQEQVMRIAS
                     DLAGFSMGEADVLRRAMGKKKVKELMDQRDKFVDGAARNHISAENATRLFDIMESFAG
                     YGFNKSHSAAYALITYQTAYLKAYFPEEYMCAFLSSVIDHQDRVVFYIKECKKMGIDI
                     LPPDINESFENFTVSAGRIRFGLGAIKNVGINAVKSIVQTRKQGSFTSLFDFCQRIDL
                     SQINKRMMENLIVAGCFDALHITRKQALSIMDECMELASQIKLNENSQQISLFGDSSE
                     LIVEEPRSKVKGEMDVRELLSREKEVLGFYVSENPLDRFKDIIPLIVSEELGELEKDT
                     HEGYVRVAGLASELNKKVSRKGEAYARFVLEDLSGRMEMMIFPSAYQKTINKIEPDQL
                     LVVEGFLDRRDEMPKIAVRNVGVPGKEVKDLHIRLPYEKDDDQGRQELLGKLAQYPGN
                     IEVVLHLPNKKIVVLTEKFNVAANLQLKNDLTRLYGKGSIWYS"
     gene            29330..30286
                     /gene="pfkA"
                     /locus_tag="P5549_03205"
     CDS             29330..30286
                     /gene="pfkA"
                     /locus_tag="P5549_03205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRW12113.1"
                     /translation="MHKVAVLSSGGDASGMNAAIRAVVRTALYMRMRVYGVRRGFAGL
                     IEDDMVEMNAGSVADIIQRGGTILQTSRSELFKTDEGRQKASQNLLNRGIENLVVIGG
                     EGSLKGAYHISRLGINAIGIPATIDNDIVFTKSIGFDTAVNTVLDAINRIRDTASSHG
                     RNFIIEVMGRECGEIALAAGVAGGAESILIPEIATDLDAVIDKINQGSKRGKLHSIIV
                     VAEGVYPPLELRNIIERRTGHDTRITILGHTQRGGSPTAQDRILASCMGKAAVELIAS
                     QNRNQMVAADGKHFFALPLQEVINGNKTLDLEMFAIARMLSI"
     gene            30301..32049
                     /gene="pyk"
                     /locus_tag="P5549_03210"
     CDS             30301..32049
                     /gene="pyk"
                     /locus_tag="P5549_03210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRW12114.1"
                     /translation="MRKTKIICTIGPASEDAEQLKKMIINGMNVARLNFSHGTYAEHE
                     IRIERLRQAARELDRPLAIMLDTKGPEIRTGKLKDGKVNLHSGQPFVLTNREVSGDEK
                     EVQVSYAPLAQEVGKGSTILLADGLIHLQVEDCRDEDVMCTVVNGGVLGEKKGVNVPG
                     VRINMPFLSPKDVADIHFGIDHGVDFIAASFVRSAEDVLEIRRILEERNADIDIVAKI
                     ESQGAVDNLEDIIKVADGVMVARGDLGVEIPAEDVPLVQRKIVAQCNYAGKIVIIATQ
                     MLESMINNPRPTRAEVSDVANAIFDGADAIMLSGESAAGHYPLAAVETMARIAKRAEE
                     AIDYKEILAKKGPQGKLSVTDAISYACCTTASNLQIASIITPTQSGLTARSIAKYRPQ
                     ADIFAVTSREKVLRKLTLVWGVHPVHARKTNGTDELLDEALNCCLDKQYIKRGDMVVL
                     SAGSPTGISGGTNLIKVHIVGEILAYGMGIGNHPVSGPVRLILHEEDLSKIQSGDIVV
                     THAASAILSSYLDKIKAIIAEAGGLTSDAAIIGLNAGIPVIVGCDEATRIFKDDMLVT
                     VDTAHGRIFNGLTKVL"
     gene            32078..32992
                     /locus_tag="P5549_03215"
     CDS             32078..32992
                     /locus_tag="P5549_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12115.1"
                     /translation="MFNINQHIAVVEFHGFEYPHCNCLLVRDDINCLLDNSPGRADLE
                     NLCRQPVDIIVNSHGHIDHYLCNQYFPSSRVYMHPADQAIAQSAEKYLQAFGLTTLTH
                     NPLLQQLYLKAVKYRTAIIDEDISEGQKLNCGATKIEVLHLPGHSPGHCGFLFPDQGF
                     IFTADIDVSNFGPWYANLNSSIIDYVQSINRVIDLKPDFIISGHGQAMIKDGLMKKLT
                     DYRDIIFARQERIVELLFRGYHRLPDLARQCPIYIKFPRPREIFYIYEQVMILAHLRY
                     MEEIGQVIRDGDQYYLKDGIRHSKSYPL"
     gene            complement(33121..33978)
                     /locus_tag="P5549_03220"
     CDS             complement(33121..33978)
                     /locus_tag="P5549_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRW12116.1"
                     /translation="MDVNKVAVLGAGTMGMGIAWAFAGAGKKVVLYDIKKEFSDKALE
                     RIGKGLAKAEEKGQVPAGAKDFVTGNITPTGTIEDIKDVDYVVESVFENMEVKKNLYA
                     QLDQILPPEIIIATNTSSLSITEIAAITKRSDKVIGMHFFNPANIMALIEVIPALQTS
                     EETLAVTMELSKAIGKKPIKVKEGPGFVVNRILIPGMNEAAFILYEGLATVEDIDKAM
                     KLGAGWPMGPFTLADMVGIDISLAVCNTLFNETGDSKYRPAPILKQMVRAGYLGRKTG
                     RGFYDYSGK"
     gene            complement(34129..34356)
                     /locus_tag="P5549_03225"
     CDS             complement(34129..34356)
                     /locus_tag="P5549_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HRW12117.1"
                     /translation="MRISAPNKLFGKIMKVHTGQDMAEIIVDVGDQAVTATITASAYS
                     DMDLQEGDAVFAVFNSTVVSLIKDHHESQSE"
     gene            complement(34514..>35041)
                     /locus_tag="P5549_03230"
     CDS             complement(34514..>35041)
                     /locus_tag="P5549_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRW12118.1"
                     /translation="ARLMQLCNVHNLPILSLIDTPGFMVGPDVEQRAQVRHVCRMFVV
                     GSHVTVPYFSIALRRGYGLGAMAMAKGGFHESFFTAAWVTGEFGAMGLEGAVKSGFKR
                     ELAAIEDEQEREALYNKLVDKLYERGKAVNMASYLEIDAVIDPADTRTWIMRGLKSAP
                     RRKDGESLLPFVDTW"
BASE COUNT         9267 a         7649 c         9107 g         8918 t
ORIGIN      
        1 tcttacatga gtttggatca tataaacgca cagcaggcgc tgcaacgact caaagaaggc
       61 aatgaaagat tccgcagcaa tctgcagtcc aaagactgct gcagtcagcg ccggcaggaa
      121 ctgcttacca agggacaaac cccttttgct gtcatattgg gttgttccga ttccagaatt
      181 cctccggagg tcatttttga tcaggcattg ggtgatattt ttgtcatcag agtggccggc
      241 aatgtagttt ctgcgattga gcttggcagc attgaatacg gagtcgagca tttgcatact
      301 cctttgctgg tcattctcgg tcacgacaac tgcggagcgg tcaaggcgac ggttgatggg
      361 ggagaagccc caggttccat tggagcgatt gtagatatga tcaagccctc cgtggaaaag
      421 gccaaggccg ccggtgccag tggagatgat ctttacgaaa aggcttgtga agaaaacctg
      481 tgggcgatgc tggcgattgt caagcagagt ccgattattg aagaaatggt cgcccatggt
      541 cacttagaga tcgtgttggc caagtatctg caaaacagcg gcgaggtagt gtttttctaa
      601 gctcatgacg gagcgctggt caatgtgata gcgctcgtcc acatagacgg aggaaacgtt
      661 ggagaggatc ctaacactat catagtgatg tagcgtctta cctgattatg gccagcacat
      721 ttttctattt gtgaaaagaa aaagcattgt attgggttga ccggacagag cgtttgcata
      781 ccgaatgatc cagtaaaaac ataacacttg gggggagttt ttgtgctcaa atatcatttt
      841 gaaattctta aaacgactcc actaaagggg cataagctgc agggcaccaa agattatgaa
      901 acccacgtaa cgatagaaaa agacgataag ttgatctttg acgaagtagt gcaggtgcgc
      961 aaaaacaaag aaggcgtttt tcctgatctg tccatggcca ataaaaaaat tgcggcggct
     1021 tccattcgcc gcgaactggc ggaaaaacta aaggaatttt ttaaacgaat gaaataaaag
     1081 gttaaggcct gctcatgctg attagcaaag caggcctttt cttcatcctc ataggcggag
     1141 tattgttcgc ctgagagcgg cgcttccttg ttcttatttt tacaaaggaa cagtattgac
     1201 agaataaact aaatagaaat aaaataaata tatcaaagtg atatatggag aataaaacta
     1261 tgaacaatta tttcacggtt gattatatca ttctaggctg tttgagtatt gagcccatga
     1321 gcggatatga catcaagcgg ctgattagta tcagcacttc cctgttttac aatgccagct
     1381 acggcagtat ttatccgacc ctgaaaaaga tggaggaagc cggactggtg gtggcggaag
     1441 aagctgtgga aaatggccgc tttaaaaaaa tctatgcctt gacagccgag ggacacaagg
     1501 agtttatggc ttggctggag gagcccccca aaccgattgt catcaaaaat gatatgttgc
     1561 tgcggctgtt tttttatcgc caactgcccc gggaaaaggt actttccatt accgctgcgc
     1621 acattgagca gttaaaggct tttaaattgg aactggatga aatagaggcc actgaaggaa
     1681 gttgggcgga caagttccaa tatgatacgt tgcgctgggg aaaagatttt tatgattttc
     1741 agatcagttg gtatgaaaag cttttatatc aattggagaa tgaaacgagc caatagacta
     1801 accgttttga tatgaacgta aaaggaaatg gccatcaagc tgtttggtta caggagggag
     1861 tgagtataaa ctgcgaaaat aagtatactc tatttcaaaa aggcttgaga gaaaggatga
     1921 tgaaaaatga aaaccaggat tactgaaatg ttagggataa aacatccaat catttgcgga
     1981 ggtatgctgt ggctgtgcaa gcctgatctg tgtgcggcca tttccaatgc cggcgggctc
     2041 ggcaatatca ctgcgggcaa ctatgatacc ggggaagaac tgcgggctgc cctgaaacag
     2101 accaaagccc tgacagataa accatttggc gtaaacgtta ccttgctgcc gtcttttcgt
     2161 atcacccagg aaacctatga tgattatttc cgggtctgct gtgaagaaaa agtagccgcc
     2221 attgaagtat cgggtgcgcc tgccaaacgt tatctggatc aggtgcatga ggctgggatt
     2281 aaaatattcc acaaggtcgg ttctttaagg catgcgctga atatcgaaaa acttggttat
     2341 gatggcatct ttgcggccgg catagaagag ggcggccatc cgctcaacga cgatgtaacc
     2401 accatggtgc tgacgccgcg cattgctgaa tcggtcaaaa tcccggtcgt gaccaccggt
     2461 ggcatcgctg acggtcgagg actggcggca gccttggcct taggcgcgga aggcgtgatg
     2521 atggcgaccc gttttatcaa caccaaagaa tgcatcgtcc atgagaatat caagcaggaa
     2581 atcatcaacc gtcaggaaca ggataccact ttgatttgca aaaccctcaa tctgcaaggc
     2641 cgggcgctga aaaatgaact gacccaacag gtgttggata ttgaagcgga aggcaaagac
     2701 ctgggacgga tcttcccgct gattaccggg caacgggtca aagaggcctg gaataccggc
     2761 gatgtaaatg gtgcggcttt catggtcggg cagtcgatcg gcctgatcca ggatgttctc
     2821 tcttgccagg aattaatgga tggtatggta aaagatgcgg aagccatcct gagaaagaat
     2881 ctggaaagat tctgatcgtt cttttgatcg gtggtgaagt tggctgacct gactgatgac
     2941 cacattggtt ctatatgaaa ggggggtgtt tatgtccgaa tatgatgttg tagtaatcgg
     3001 tgctggcaac ggaggcttaa ctgcagctgc cagccttgcc caaaaaggaa tgaaagtatt
     3061 gatgctggaa cgacataatg ttccgggagg atgtgccacc agttttatgc gcggccggtt
     3121 tgaatttgag gtggctctgc accaactgag cggatttgga acaaaagata aaccgggccc
     3181 gttaagaatg tttctggacg gactgggagt ggtggatcaa ttggattggg ttgccatgga
     3241 gaatctttac cgggtcgtcc tgcccggaga actggatctg attttaagca gcgatcggga
     3301 aaaaatgatt gccaccttgc aggaaagttt cccgcaggat agccaggcga tcgctgaatt
     3361 cttcgatctg gtctacaagt tctttcatga aatcatcagg gccttcttta tgaaggatcc
     3421 cgaggtcagc aaggagaaat atccgctcta tttcaaatac agtcttaaat caacgcagga
     3481 agtcatggat gagttcttta ccgatccgct gctccaggct gccatatcag tgtattggtg
     3541 ttacgtcggc ctgggaccga accaactgac tttctcggat ttcgcggccc tgatgtttgg
     3601 ctatttggag ttcaaaccat ttcatttaaa aggcggatcg caggctttat ctaatgcgat
     3661 tctagatcaa ttcctaaacc atggcggaga agtgagattc aactgtgccg cccagcgaat
     3721 cctggtcgag aacggacatg tcaagggagt tgccactgaa gatggtgatg taatcgactg
     3781 taaatatgtc gtttccaatg cctccagtgt ggaaacgtat gttgacctta tggaaccatc
     3841 cccggcggca gaccaggaga tccaaaagat ggccggcagt acagtcggac cttcctcgct
     3901 gacgctttat atcggtttgg attgctcccc tgaggaagtc ggaatcaatg aagccaccaa
     3961 tttcattctt accagtgcag acatgaacgg tggctacgag cggtttaaga cgctggatac
     4021 cagcgatgac atgcttttgc tcacttgtta taacctgctg gatccaagtt ttgccccggc
     4081 aggctgcagt cagatcgtaa tcgtgggttt gaaatatgcc gatccgtgga ttgaggtgcc
     4141 tcccgccgaa tactaccaga ttaaatacca ggcggctgaa ggcttcctgc aaagcgcaga
     4201 aaaagtattc ccaggattgc gcgagcatat tgaagaaatt gaagtggcta ccccgatcac
     4261 ccatatgcgt tttctggggc aacctggagg agccatctat ggctttgata aatttgccaa
     4321 ggaaaactct ctttttacaa atcgtaaatc tcccattaaa ggattgtatc aggccggtgc
     4381 ctgggctggc agcggaggct tccagccgac attgacatcc ggcggtcagg cagcccgggc
     4441 gattgtaaaa gattttaggg gggtggaaaa atgagagact tccgtgaaga tattgaaggc
     4501 tatggtgaga tcaagcagga gatggagata tctcgtaaat acgggatcaa ttatcgggtg
     4561 caaaagaacc gccgggcgga tgtagtaagc cgcctccatc ccaaggtttt aaaacttagg
     4621 ttggctgata taatattgga gacgcctacc accaaaacgt tgcgtctggt cgcccgcgag
     4681 ggaaatctac cgccgtttca ggccggacag tacatcaatc tgcaggtaga gatcaacggg
     4741 atcaggacca gccgcccgtt cagtatttct tcatctccgc ggcaaacggc ttattatgat
     4801 attaccgtcc gtaaaattga aaacggattt gtctcagcct atttgtttga cgatgtaaga
     4861 gtgggagatg aatttacaag ttccggcccg gccggcaact tctattataa tccgttgttc
     4921 cacggacaaa aattggtctt tttagccggc ggcagcggag tcactccctt tatgagcatg
     4981 atccgcgaaa atatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctgcc
     5101 cgcaaaatcc ggcatgaaat gtttggcaat cctgcagata taacggctca accgggatgg
     5161 ccgcgggaaa tacgaaaaga ggctgccttt acggttaagg tcgagggcca taagagtttg
     5221 gctgcccagg caggagagcc gctgctgatt gcgttggaaa aagcaggact gatcgttcct
     5281 tctctgtgtc gctcgggaga gtgcagcctc tgccgcgtac aactcttatc cggcaaagtg
     5341 tttcagccgc aaggtgtgct gatgcgggaa agtgacaaaa aatacggtta catccatagc
     5401 tgcaaggctt atccactgga ggatctggtc attcggctgt aaattttctt acgatgttcc
     5461 tttgtatttt aaaaagtata cactaaagga gaaaaagctg atggatctgc tgaaaattta
     5521 taagcaaggt gaagatgatc cgaatgcagc agccttctac ctggagaacc cgcaagccgt
     5581 tctcatggat ttctccggaa agttcgaaaa aataaatatg taaaaggtaa aagggaagga
     5641 ggcgcaagca tgggtgaatg gaaaaagacc agctgcgtat tatgcgcgca gagctgcgga
     5701 ttggagatgc taatcgaaga caatcggatc atcaaggttc gcggagctaa agacaaccag
     5761 cgttctcaag gctattgctg tcgcaaaggg ctgaatatgg cgcatcatgt gcataacgct
     5821 gatcgcctca agtatccact caaaaaagtg ggggatcatc atgaacgcat cagttgggaa
     5881 caggccctca cggagattgc tgagaaattg ctgtccataa aagaaaagca tggcggcaag
     5941 gcgatcgctt atatgggcgg cggtgcgctg ggcggtcaga tgcaggtcgg ggttggattg
     6001 cgtatgttgg gttttctggg ttcccgtaat tattattctt cgattaccca ggaattttcc
     6061 aacagcttct gggttgacgg acgagtgacc ggcaaacaga gtatcgttac catgccggat
     6121 gcgcatcatg ctgatacatt ggtggcttgg ggctggaacg gctggatgag ccatcaggaa
     6181 cctcgcaccc gcgaattgat cctggaattt aaaaaagacc cggataaaaa gctgatcgtg
     6241 atcgacccgc gccgttcgga aacggcggaa agagccgaca ttcatctgcc catacggccc
     6301 ggcactgaca gcatgctgct gaaagccatg attcgcatca ttcttgacaa cagctgggaa
     6361 gaccaggcgt ttattgccgc gcatgtcaac ggctgggagg aagtaaaacc cctgtttgac
     6421 ggatttgatg cgcgacgtgc ggtcactgaa gtctgtggcc tggattatga aacggtgctg
     6481 gaggtgactc gtttgctggc ccagaccagg tcttgcatcc atcaggatct gggcatttat
     6541 atgaaccgca attccagtat caataactat atgctgcata tcctgcgggc catcaccgga
     6601 cgcttttgtg tggaaggcgg ccagatcata ccggcattcc tttttcccat gggcagcaac
     6661 agtgatgagc gtaatcccaa agtctggcgc acagtcaaaa ccaatatgtt tccgatcctg
     6721 ggatcttttc cgccggcggt atttccggag gaagtgctca atgatcatcc agacagaatt
     6781 cgggctctga tcgtcagtgc cagtaacccg ctgcgttcct atcccgatac gcaggcttat
     6841 gaaaaagcct ttgcggagat ggaactctcg gtctgtttgg aaatcgtcta caatgaaacc
     6901 gctcgttgca gtgattatgt gctgccttgt ttgagctaca tggaatcata tgatacggtc
     6961 tgcttcaact acagctatcc ggatctttat ttccatatga agcagccggt gctggagccg
     7021 ttgagcagtg aaagcaggga aggctcgcgc atcataatgg atctcatcaa agccatggga
     7081 tttctgccgg agtttccaca gtcgctttat gatgcgggca aagagggtat ctctgcttat
     7141 ttgggcgcga tgggcgcata tgttcaggaa aatccgtccc aggccaagtt gattcccctg
     7201 atattggcgg aaacattagg aacagccttg ggttctgtca atcaggccat gattgtagga
     7261 ctgctggtat cgagcagcaa ggaatttaaa gccggcgctg tcgccatggg gtatccggct
     7321 gatttttcca tgattgaagc catgtacaag gatatcctgg ctcatccaca gggactcatt
     7381 ctctccagat tccagggaga caactttaac atgctgaaaa ccgaagataa aaagctgaat
     7441 ctggtgatcg aggaactggt tgatcagatg caagccgcca gcattgaaaa tgaaatcgaa
     7501 aaattgaaaa tgccggctga atttccgctc atcctgcatt ccgggctgca tcatgaaacg
     7561 gttgccaata ctagtttgcg caatccggaa tggaacaagg acaagcgctg tatgaccatg
     7621 ttgatgaatg aagttgatgc tgcccggcta gatctggctg acggggacaa ggccatcatc
     7681 accaccaagg caggctcggc cgaagtggaa gtggaaatca gttcgcgcgc cgctgtcaac
     7741 tgtgtatatg tccgtcacgg gcagggtttg atctatgatg gcgtaaaata tggcgtcaat
     7801 gtcaatgatc tggtcttgac cacagaccgg gacgaaatgt gcacccccat gcaccgacgc
     7861 attccctgcc gggtggaaaa agcatcataa acccggaacc agtgaccatc cctgagccaa
     7921 cagaaaagcc aacaagccca tatcatcaat catggtatgg gcttgctcac tttattggca
     7981 aagttgctgg tatagtgctg catatgcgtt taattctttc tccaaactga acttttcctc
     8041 caccagtttt cgtgcggcct gccccatcat cttggtgcgc cggggatggt ccataaagat
     8101 tcgggctgcc cgggcgaact ccttttcatc atgcacgtaa aagccctccc gaccctcttc
     8161 aataatatgc aaattgccgg gtacggcgct caagatgcag ggtaatccca aactcattgc
     8221 ttccagcgcc ccctgcggct gaccttcggc atgggagctg ttgacgacga catctgcgtg
     8281 caggatcagt tcccgcacgc tggcatgagg tatttcaccc atataggtaa cccagggcaa
     8341 ggcgtcaatc cgctgcaaca ttcgtcggct gtagtcggga tctataagcg ctccgataat
     8401 caccaatttt aattgaggat aatcaggttg aatattttcc agtgcgttga gggtcaactc
     8461 aaaatttttt accggtctta acccgctggg gaagagaaaa ataaaatcat cctgtttaaa
     8521 atcaaggggc agaaatgttt cacgtgaaac attttcgaaa aaaatccctt gggggataac
     8581 caccaatcga gccgccaggg acgggaaagg aactgcaatt tgagtggtga aatcctgatg
     8641 aaaaagcaca attttttgcg cggaaaacaa gctcctggca agcaccggcg aatcctttcc
     8701 ggatagatca aaatgcaaat ccgtaccggt ggtggtaaga agcaccggga tctgctgaat
     8761 ctgaggcagt tgatctagca aacgacccca atgcagcgca tgaaaggcat gaatcaatcc
     8821 atagcatcca gagtccagct gctgctgcag ctgtggcaag acatcgcttt cctccatagt
     8881 gatccaatca atcatgaaac cctgcttttc caagccggtt ttgaggcgca tgctggtgat
     8941 gctgttgccg cgctggctgt gccggtaagg actgatcatc aatattttcc tgggtcggtc
     9001 agatatgatg gtatgcataa cccctccgct gcaagcaaga ttaagatcgg gttttaatca
     9061 attgaaaaca ctccgcaaac tgacccgttc cactggtgac ggcgcgatag cgattcaggc
     9121 cttggaccgc ttcagcatag gcgatatccg ccagttggga cttgtgttcg cccaaagcag
     9181 ctagtttttt ctgcatatat tcactgatgt cgacggtcag gttatagcgg ctaagcggag
     9241 tccatacttc ataggcaatg atctttccgc ctccccaggg ttttccgcag gcaggaaacc
     9301 aaggcccggc agctcgttgg caggcttcca ggatcaactc gcagctttgc cggtgatcac
     9361 ggactgcttc ttcgccatgg ggcaggaaaa ccatgcttgg ttgttggttg cgaatgacgg
     9421 tgaccagtgt atccattaat tctttgctcc agacaatata gccatccggt tggcgcagaa
     9481 agatgacgtc gctgactcca atcatggctg ccgcccgtga ggcttcttct tcccgcaaag
     9541 cagccagctc tgacggattg taagacagac tgccgttttc tccggaagtc agataaacaa
     9601 tcgccgcctc atctccgtgg gccatatgac gggcaatcgt tcccccgcag ccgatgatgt
     9661 cgtcatctgg atgaggggcg aagatcagca gtttctccat tataattacc tccgcgtagt
     9721 tgataatcac ctggatttaa gccaggatga gtattcttag tatagcgaaa atcaagggtg
     9781 atgataagag tgccaggatc agcacctttt tataaagtat taacattaaa atcaataatt
     9841 gttataatat tttaaaagtg aaatatagag aaacgtaaaa atatttgaaa ggaaggtatt
     9901 tttatgcagt gggaaacagt caatcttgag attatggata gcggtgcagc catcatgacg
     9961 tttaatcggc cggatttcct gaactcggtt aatgaacagt tttgtaaaga tgtaaacgca
    10021 gcctgcaaag aagcagcgga taataatgat atcaaagtcc tgatcgtgac aggggccggc
    10081 aaaggatggt ccagcggggg agatgtttcc accctgtaca gtatgacgac gccgctcaac
    10141 gccaaagaaa cttatgatgc ctcagccagt gtggtgacag cgatctatga actgcaaaaa
    10201 ccggttatcg ccgccgtcaa tggggtggta gccggtgcct caacggctac ctgcctggcc
    10261 tgcgatttaa taatcgcctc ggagaaagcc cgctttggtt tcacctttat gcagctggcc
    10321 ttttgtccgg acagcggggc ttcctatttc ctgacccaaa aggtgggcta tcacaaagcg
    10381 gcggagatca tctggtttgg aaaaattcta aatgcggcgg aggccaacga gttgggtctg
    10441 gtcaacactg ttgtagccca tgacgaactg atgaaaactg ccgttgaatg ggcggagaaa
    10501 cttactcgtc tgccgctggt gaccgtcgga ctggataaaa aattgttgcg ggcatccttg
    10561 aaaaatgatt actatcagca atgcgaactg gaagcgctca atcaagttct atgctggagc
    10621 tcggaagatt ttaaggaggg ctgtcaggct tttgtagaaa aacgcaaacc taatttcaaa
    10681 ggaaaataaa tcaaatcaga ggctgggatc aaatttgatg ccagcctttt ttgtgaaaaa
    10741 agaccctccg gaataaagac cggagggcaa aagaaaagga ttggaattag acactagaaa
    10801 agccattcgg ttgtcagtct cccatcagaa gcaaaaagcc agacccatgc gcacaggtct
    10861 gaccaaaaag gctgccaaaa aagccttccc agcaaactcc tttccgcaat gggaggcata
    10921 accgtttcca gctataccct aacggtcata agaccatggc caaaagcttt agatcttagg
    10981 actcggagac tgaagactag ataaatggac ttttctaatt agaataattt tacactgatt
    11041 gtgattatga tgcaataaag attaggtagc aaagagaatt cacaatggag tataataaaa
    11101 cggaacaaat aatattgcca ggcaggattt cgataagtta tgtagaagtt ttcacaaagt
    11161 acggctttcg ttttccttct tgcggacggt atgccaaact gccaaattaa atatggtctc
    11221 cgaactgtta acctaaagct gtcaggctat ggtaagcagt cattgggtga tttcggaaac
    11281 ggatttgcct cccattgcgg aaaggagcaa ctgctatact gctggtttca ggaaaacgca
    11341 gaaaccgctg ttgtagttgt acttttctat ctgcagtggg tttttgtttt tttagataag
    11401 tttatacgga taggaggtga gagtgtgagc agaattcaag aaattattgc acaatatggt
    11461 gacaaacagg gcggaattat tgaagcttac catgctgttc agaaggaatt caattatctt
    11521 cccgaggagg cagtcattga agctgccagc gtttttggtg tgtctgaagc ccaggcctat
    11581 ggtgtggcta cattttattc ctacttgtcg gtgaaaccgc ggggcaagta tatcatcagg
    11641 atgtgtgaaa gtgctccctg tcatgtggct ggcgcagacg aggtgattca agcaattgaa
    11701 aaagagctcg gcattaaagt gggtgaaacg acagcagacg gaaagtttac cctggaactt
    11761 acggaatgta tcggacagtg ccaggctacc ccggtgatta cggtaaatag tgaaccgatt
    11821 tttgatgtta ccccgcagaa ggttccggag atattgagtg ctttaaaata aaaacaccgg
    11881 atgcctgtaa acaaagatag agagggtaaa agaaaaggat gggataaaaa tggcgaaaga
    11941 aatgcgtgtc ttgctcgaat acgcagatcg aatcgatccc gccagcgctg aagattatat
    12001 caaggtgggg ggatacaaag gtctggaaaa agcgcgcaag atgaacccca gggacctcat
    12061 ccaggaggtc aaggattcca aactgagggg acgcggcgga gctggattca atgccggtat
    12121 gaaatggagt tttgttcccc agaatgtgcc gatcaaatat gtggtctgca accttgatga
    12181 aggggaaccg ggaacctaca aggatagaat tatttgtgaa aaaaatgccc aagccctgct
    12241 tgaaggaatg gccatttgcg gacatgcaat aggctccaac caaggatata tttattgccg
    12301 cggggagtat cccttcgtcg tggatttatt acgcaaggcc atcgacagtg ccagcgaagt
    12361 gctgggggat tttaacattg aagtcagaat gggtgccggt gcttatgttt gcggagaaga
    12421 aacggcactg attgaatcca ttgaaggaca tcggggtgag ccccgtttta aaccgccttt
    12481 cccgggagtg gaaggtttgt ggcaggtgcc aacggtggtt aacaatgtag aaacctacgc
    12541 ttgcctgcct tatattctgg ttaatggtgc gcaatggttt gccggcattg gttcttccag
    12601 ttatccgggc accaaagtac tgtctttgac cggagatgtg atcaaccgga cctattttga
    12661 agttcccacc gatatgaccc tgcgggaagt cattttcgaa ctgggcggag ggataaagaa
    12721 cggcaagaag ttcaaggcag tccaagtcgg gggaacgtcg ggagcgttta tccctgaaca
    12781 ttatctggat accaaaatag atttcgattc catgggcgcc attggcgcag cccttggttc
    12841 cggagccgtt tttgtgctgg atgagagccg tgatatttta gatgtggcaa cgcgtatttc
    12901 caaattcttt gaacatgaat cctgtggcaa atgtaatcct tgccgggaag gtactttccg
    12961 cagcagggaa atattggagc gcttaaatgc cggcaagggc agcgaagctg accttgacaa
    13021 actgctgctg ctctccaaag tgatgcaaaa ggcatgtttg tgcggattag ggcaggctgc
    13081 tccaattccc accgagagta ccatcaaaca tttccgtcat gaatacctgc gcaaggtgca
    13141 gtaatttgaa caggaaggag gagcaaccat gattagcctg accatagatg ggatggaagt
    13201 tcaggtaccc cgcggcacca tgctgctaga tgcatgccgc caggccggag cggatattcc
    13261 caccttttgt tatgactcgg atttaaaact ggccggttcc tgccggatgt gtgtggttga
    13321 atgtaaaggg cgccgcaatc ttttggctgc ctgtgtaacc ccggctgaga atggcatggt
    13381 gataaacact gaaagcgccg atgttgttga aaccagaaaa gtgattttgg atctcttgct
    13441 ggcccgtcac aaactggaat gtcatgtttg tgagaaagac ggagactgca agcttcagga
    13501 ctattgctat cggtatggcg taaaagaatc ctcactcggt gaagaaggac cgcattatta
    13561 caaggaagat gccaatccct ttatcgagcg ggattatgac aagtgcatca tgtgcacccg
    13621 ctgcgtacgc gcctgtgaag agattaccgg tgcctgcgcc attaatcttg aagaccgcgg
    13681 tcatcatgtg catattgcta cagcctttaa tggactcctg gaggattctc cttgtgtctt
    13741 ctgcggacag tgcataatgg tttgcccggt aggggcattg accagcaaaa tatcagccgg
    13801 caaaggcaag ccctatcagg tcaaaaaagt cccgactacc tgtacctatt gcggtacggg
    13861 ctgtaccttt gaattaaacg tgaaagacga cgagattgtt ggtgtgactt ccatgcgcaa
    13921 ctatcagcag agccccgtaa acaatggagc gctttgcgtc aagggacgtt ttggctggga
    13981 tttcgtcaac tctcaggaaa gactgaccac gcctttgatc agagaaaacg gcgaattgcg
    14041 tcccgcttcc tgggaggaag cgctggactt tgccgccggc aagattaaag aagttaaaga
    14101 aaagcatgga ccagatgcct tggccatgtt ttcttcggca cgcatgacca atgaagagaa
    14161 ctacctggcg caaaaactga tgcgggcagt tatcggcacc aataacgtcg atcactgcgc
    14221 ccgtctctga cactccgtta ctgtcgccgg tctgggggca gcatttggga gtggtgcaat
    14281 gaccaactcc attaacgagt tacaaaacga tgccaaagcc atctttttga ttggtaccaa
    14341 tacaactgaa aaccatcccg taattggcta caaaatcaag agcaacgtcc gcaagaacgg
    14401 tgccaaattg atcgttgctg atccgcgccg gattgaactg gcggagatgg ctgacgtcta
    14461 tatgcaattc agaccaggta cggatgtggc cctggtcaat gccatgatga atgtgattat
    14521 cagcgaagat ttgttggaca aagagtttat tgccaaccgc accgagggtt atgaagccat
    14581 ggcagcggtt ctagccagtt atacccccga agcagcggaa aagattaccg gtgttcctgc
    14641 tgcagacatc cgcaaggcgg ctcgcattta tgcggaagcc gaagttgctt cgatttgcta
    14701 tgccatgggt ttaacccagc acagcaccgg tacggacaat gtaaaaaccg tctgtaattt
    14761 agccttgctc accggtaatc tcggcaaacc cggcggaggg gtcaacccgc tgcgcggtca
    14821 gaacaatgtg cagggcgctt gcgatatggg tgcattgcca gtatgctatc cggcctacca
    14881 ggccgtcacc aatccggata tccgcgagaa attcgaaaag gcttggggcg tacccctttc
    14941 ccccagcaat ggtgtaaccc tgaccaaggc catcaatatg gcgcatcatg gtgaaatcaa
    15001 gagcatcatt gtcatcggtg aaaatcctat ggtcagtgac cctgatctgc accatgtcga
    15061 ggaagccctg gaaaaccttg attttctaat ggttcaggat atattcctga ctgaaaccgc
    15121 ccagaaagca gatgtggtac tcccgggcgt aacttttgct gaaaaagacg ggacattttc
    15181 caatacggaa agacgggtac aaagagtccg ccaggccatc agcccccgcg gcgatgcccg
    15241 gcaggattgg cagattcttt gtgaactttc cacacggctg ggctatccaa tgtactatgc
    15301 ctcaccggaa gaaatcttca atgaaatgcg tacggttact ccttcctacg ccggcattac
    15361 ctatgagcgt atcgaagaat gcggtctgca ttggccctgc ccgaccgaag atcatcccgg
    15421 tacgccgatt ctgcataaag aaaaattcaa tcgcggactc ggactgttcc atgccattga
    15481 cttcaaggaa gcagctgagt tgccggatgc tgaatatccc attctgttga ccaccgggcg
    15541 tagtcttttc cattaccaca ctggtaccat gacccgccgc tcggttggtt tggatgctat
    15601 tcagccggaa aatgaattgc agattcatcc gctgaaagca gcggaactgg gaatccatga
    15661 cggtgaaaag attcgggtca cttcacgtcg cggcagtatt gaactgaaag ccagggtgac
    15721 ggatattgta gatgaaaatg tcgtttttac cttcttccat ttcgcggaag cggcagccaa
    15781 cagactgacc aacgcggcac ccgaatcact cgatccggta gccggcattc ccgaatacaa
    15841 ggtatcagca gtaaaaattg aaaaaatcta gatatctaaa gtaaaaagcc aaggggctgt
    15901 ctttaaaccc ttcagaggga ttgggacggc ccctttttat cagcacatgg gcttgacaag
    15961 tcatcgattg acaagtctgc tgcttttggt ttatatatga gtggatccct gttgcattaa
    16021 ttccgtttcg aggaggtaca accgcttcgt tgataacaga tatggaaacg caaacgctgg
    16081 ttgttgagat ctgtacccgg gtctggttat ttccaaaagg agattgatca tgtttataaa
    16141 taatacctgc aacggttgtg gaatatgcgt tgatttatgt ctcggcaagg ccatttatct
    16201 ggcagatgat aaagcggtta tcaactacga aaagtgcctg ggctgtggaa tatgtgttaa
    16261 tttctgcgac ttgggggcaa tacgggagga ttgggaaaaa atgttctatg ggatgggcgc
    16321 tgaataagct tgataatgtt tatatttttt tctggtaggg aagacccatg cggccaatga
    16381 tgtattgccc gaattgatgc agaattcggt tcagtttttc cgcggattct gtttcttcta
    16441 caactacggc gatgattgcg acctgcgttt tcaatgtcaa cacctcgatt cataaaaata
    16501 aaaaagcgcc cggcaaggcg cacaaaaaca ctggaaatct tgcacttaca cctcggatag
    16561 catctttacg tgtcagattt aaccatatcg ctttgttaaa agacgcatcc cgtaacttag
    16621 cggtattctt tttataccat gttgaaacaa gcatacaaaa ctaactcata atatatacag
    16681 aaagaaaatt ttgcatttct ggttttgtgc cacattcata aaacatcccc ggaatatgtc
    16741 atcatgccat tataatccag agcccagatg ttaatatgga atatgatacg tttccatgga
    16801 acctgcgatt ggaattacct ttgttaaact accgcatact ataaaaagaa tatctgcaaa
    16861 ggagcaagat gatgtcattg attaatgcag gcaagttata tccggcactt gattcgatcc
    16921 ccggtgaatt ttgcccgccg gtttttaaac cggacgcaag gtacttggtg aatggcagga
    16981 tcattactgc ggcaagccgg gaagtatttt cgcccattca attccattcc gggcaggggg
    17041 tgcagccgta ctgcctggga gtcacaccct ggcttgagcc ggatgaggcc atggaggcct
    17101 tgctggcggc tgaaacagct tatgatgcag gactggggga gtggccgcag atgccggtca
    17161 gtgagcgaat cctatgcgtt gaagagttgc ttcagcggat gcgtcctttg gaagaggagt
    17221 ttgtgctgcg ggccatgtgg gagattggaa aatcttatca ggcttgcaaa gacgaattta
    17281 aacggacgat caaatatgct gaagataccg ttgcccggtt gcgtcaagat gatcgcaaca
    17341 gcagcctggt tcagcaattg gatggatttg aagcccagat caggcgctgc cctttggggc
    17401 cgacattatt gatgggacct tttaattatc ccttgaatga atcatttgcc atgcttgttc
    17461 cggccctggt catgggcaac acggtggtga tgaagctgcc cgcatggggc gcatgggcgc
    17521 ttgaaccgtt cctggatgcg ctctgtgaat cctttccccc gggggttgta aatattatca
    17581 atggagatgg cgctgcaatc ataacgccga tcattcggcg ggggacagtc aacatattgg
    17641 gctttattgg ttccacccgt gtggctcaca gcatcgttgg ccagcatccg catataaacc
    17701 ggatgcgcac tatcttaggg ctggaagcaa aaaatccggc gtttatcttc ccggattgtg
    17761 acctggaact taccgtgcgg gaatgcctgc agggagcgct ggaatacaac gggcagcgct
    17821 gtacggcgct caaacactta tgggtacaca aggatatttt ggatcggttt ttaactgccc
    17881 tgaatgaagc cgtcggtcag gttaaatgtg gtctgccctg ggaaaaagaa gtgatgatca
    17941 ctccgctggc ggagaaggat aaatgtgctc gtctatccga ctggattgac caagcatact
    18001 cgcatggtgc caggattgtc aaccccgggg gaggagagca cgccggcaat ttgttttatc
    18061 ctgccgtcct atatccggta accagtaaca tggatttgtt tcacgtggaa caattcgggc
    18121 cgatcatacc cgtcagttgt tttgctgcgt tggatgaaat tgctgcctat atggcgcaat
    18181 gtccctatgg ccaacaagcc agtattttta cccgttcacc cgcactggca gctccgctga
    18241 ttgatatcct ggtcacccag gtttctagga tcaatctgaa tgcccagtgc cggcgggggc
    18301 ctgacgattt gccctttacc gggcgaaagg actctgccga aggaacactc tcggtggagg
    18361 atgccctgcg ttccttttcg attcgggcgc tggtggttgc caatcaatca ggccaggaat
    18421 tgtttgaagc gattttggat aaaggcaatt ccaaattttt acgccgttga tcgcttacca
    18481 taaggcctga tggcataatt gcagctttcc gagggcagga tgttgaacag gactgttttt
    18541 gcttgtccaa acaagggaaa acacgtaata ttgatgatag gtatcagtaa agatttggta
    18601 tgggaggttg atgaaatggg tgcattgaaa attaaagaga atgtctacag tgtcggagtc
    18661 caaaatccgg atttgcgtgt ttttgatatt gttatgagga ctgaatacgg aacatcctac
    18721 aatgcttacc tggttaaagg aagtgacaaa acctgtctca ttgaaaccgt aaaagatcgc
    18781 ttttttgatg agtatcttgg caatttacag gaagtggtaa agcttgagga aattgattat
    18841 ctgatcatga atcatacgga accggatcat tccggttcgg ttaaaaagct tatggacaag
    18901 atccctggac tgacgatttt ggccagtccg acagcgctca agttcttaaa ggaaattacc
    18961 aacagcaagc tcaatgcgcg cgaagtgaat catggcgaag agctggatct aggcggtaaa
    19021 accctgcagt ttatcagtgc tccgtttttg cactggccgg acaccatgta tacctatttg
    19081 cagcaagaca aggtcttgtt ctcctgcgat tccttcggat gtcattattc cgatccggtg
    19141 atgttcaatg atttgatcga caaagatttt accgatgctt acaaatatta ttttgacatg
    19201 atcatgggac ctttcaaacc ctatgtactg gaagctctgg aaaagatcaa agatctggag
    19261 atcgacgtcg tttgtcccgg tcatggaccg atcctgcggc aaaatattga atactatttc
    19321 gatctttatc ggcaatgggc gacgccgccg gcatctgatg gagacgagcg tcccaagatc
    19381 gtcatcgctt atgtgaccgc gtatggatat actgagacca tcagcaacag cattgttgaa
    19441 ggcctggaaa tgattgctga attcaatgtg atgaaattca acctggtaga agatccggtc
    19501 agcgcggttt tagaggcact gcctggagca gatggattgc tgatcggatc tccgacgatt
    19561 aatggcgata cgctgcctcc catctgggaa ctgctgactc atctttctcc catcgtgcat
    19621 gcggatttgg tagcagctgc tttcggtgcc tatggctgga gcggtgaagc tgtaccgaat
    19681 atagaagccc ggttaaacgc tctgcgcatg aaagtcttgt ctgggctgag aatcaacttt
    19741 aaaccttccg aagccaattt agaggaagcc tttacctttg gtatggactt tgccaaggca
    19801 attttagaga aaagacagcc tcaatcccaa aagaaatggc gctgtctggt ctgtggttat
    19861 attggggagg gtccggaacc cccgtcagta tgtccagctt gcggcgttgg ggcagagaat
    19921 tttgtggagg tccgcatgga agacgagttt cgcaatgaca ccaacgatac cttcctgatt
    19981 ataggcggag gcattgctgc cctgtctgca gctgcggcaa tccgcaagcg caatgaaacc
    20041 gcggcgatta ccattctcac cgaggaatca gtcagaccct attatcggcc ggctttatct
    20101 gattatttga gcgaagagct ggaagacaaa aaattctatg tttacagcga tgattggtat
    20161 gaagaaaagc gggtggaagt gaaaacctca tgtcaagtga cggccattga taccaaggcg
    20221 agaacagtta ccgtagcaga tggcaggagt ataacttaca acaaattgat catcgccacg
    20281 ggtgcccgca gtaatatacc tccgattccc ggggccgaaa aaccaggcgt atatgcactg
    20341 cgcagttatg aggatgccca aattctgaaa aaagcgattc agtcagccaa gcgggcggtg
    20401 gtcatcggcg gcggcgtttt gggactggaa gccgtctggg aaatgatctc ccagggagta
    20461 gatgtatctg tcattgaaaa caacccccgt attatgccgc gtcagttgga cgaaggtgcc
    20521 tcggaacgtt tgcagaagct tatgacagag cggggcgtaa aactctatat gggaatcggt
    20581 accgaagaaa tcggcgggga agggcaggcc agcggggtca agcttaaaaa cgggcagtgg
    20641 ctgccggccg atttggtctt gttgtcaacc ggcgtaaaac ccaatgttga attggcgcag
    20701 gcagccggca tcaatgttga tcgtgggatt gtagttgaca ccgggatgcg caccagcgct
    20761 tcagatgtat ttgcagctgg agatgtggca caggtggtag atcgtctgat tggtttgtgg
    20821 tcgatctcaa cggaaatggg caatattgcc ggagccaatg cagccgggga ctggctggat
    20881 tataaagagc ccaccatatc caccatgctg gcggcttttg atcatgagat cttctccatt
    20941 ggcgaggtca acctgccgcc ggaggaatgc cgggtaattg aagtttggga cccgattgaa
    21001 gatttttata aaaagaccta tatacaggat ggggtcatga tgggtgagat cattatcgca
    21061 ccccaggtca acaccactga agcctttcgc agtattggcc gggatggcag cggcaagaaa
    21121 agggtgacca aatggaaatg tttggtctgt ggttatattc atgagggacc ggagccgccg
    21181 gatgaatgtc cggtttgcgg agcacccaag gataagtttg aaccgatcgg ttaagcacca
    21241 aggaaatgtt tatacccggc aggcatatag tagactgccg ggttgtttct aatgcagggt
    21301 tagcagcaag gagggccgat taacattttt ttcttcatat tgctaatcgg agtgctggcc
    21361 ggctgcctgg gggccttggt tggtttaggc ggtggcgtta ttatgctgcc gggattgcag
    21421 ttgctggcag gctttgattt gaccaaggca attggtacca ctttgtttgc agtaatattt
    21481 acgtccatat cagcagccca gggacattac cgggcaggcc atgtgcatat gcgcagtgcc
    21541 ggttatgcag cgttgggggg attggtcggc gttttactgg gctcctatgc gtttgcgcaa
    21601 tatctatgca acggcttttc ggttttggaa acgatgttag gattattctt tctgtttact
    21661 gcttaccgaa cgactaaaga cacttggctg gatatacgca gaagagggaa aaatgcaact
    21721 gacgccgatg cgggggaaaa agcaaggttt gcttccctga tggctttggg ttttattacc
    21781 ggcagtctca ctggtatttt aggaatcggc ggagggttta tcctgactcc cgggtttatg
    21841 tttttagccg gaatgtcccc gcagatggcg gtaggtacaa ccatgctgac catgctcccc
    21901 ttgtcgctga gcggcgggtt gataaaacta tatcaaggct atgtaaacct gcctgccggg
    21961 attgtgctgg ggttggggac tgcgctgggg gcccaggcgg gcgtttggtt gtcaaccagg
    22021 atgagcgcaa tcctcttaaa ggcattatta tccattgtgt tttttatcct ggcagctgat
    22081 tatttgctcc ctttgctgca aaggcagctt ttgccgtgag aattttatgg taatggagaa
    22141 tgatgaatga ttcccttgag agacagtaca cctagtgatc gttttccgtt ggttacggtt
    22201 gttttgatct taatcaacct gttgatcttc atgtttgaaa ccactttggg gtcagcggcg
    22261 ctggaagcgc ttgtctatac ttatggtctg gtaccgagcc aaatgattca ttacaacgga
    22321 cccttgacag gttatttgcc gtttatcagc tcaacttttc tgcatggcag ttggatgcat
    22381 gtaataggca atatgtgggt actgtggctc tttggtgata acgtggaaga taaaatgggg
    22441 cacggtcgct ttctgttttt ttatttgcta tgcggcttgg ttgccggcat aacccatgtt
    22501 gcgtttgaac ctggttcgca agtgccggtg gtgggagctt cgggtgctgc ggcgggaatt
    22561 atgggagcat attttttaat gttcagacat gcccgcgtgt ttacttacat tccacccatc
    22621 tttttgttta atatccctgc cggtatctat ttgggattct gggccctgac gcaattgttt
    22681 tatggaacct ttcagactct tgcacagggg cagatcgctt tctgggcgca tgtcggtggt
    22741 tttgctgctg gaatgataat ctatcgtttc tttctgcaag caagaaatga aatcacagaa
    22801 gtctaacaat gcaatgacaa gagacgaaaa gctcaccttc tgcgctggcg ctattcaggc
    22861 agaggtgagc tttggcgatt acaaatccat ggtactggcg attaggcttg ccggattggc
    22921 gagcaccggg gcgtctgtat cggcttggag aaaataaatg gtgttttcta ttctttcctg
    22981 actggctttt atttcccgcc aatggcgttc ttcttcgtat aaatttatac aatcctgcag
    23041 gtcattggcc cgaccggtga ggagataatg tttgcatgtg ctgagggcat tggtttgatg
    23101 atagtccttg tgaagataac atttatccaa aataaactga tacatctcca gcaatccttg
    23161 cgcggttttc aatttggcag ttgcctcttc aatttgttcg atacatttct ctgccagcag
    23221 caaatggttt tcttcccatt gctgcaaagc aattttataa cgttcgcaag ctttttcatg
    23281 ctcacggtag cgaatgagat aaatcttcat gcgacggttg tattctccac aagattgaac
    23341 atattcgcga tgacgttttt tggaatgatt gattgccagc aagctgctga aagaaagctg
    23401 tggttcctcc ggttttgctg gtttggcaat cagagcttcc ggttcgggcg gcaaaaggtc
    23461 ttgctgtaac agcaaatcat tgcgctgatt taaaaaaacc tgccaataat cacaatggcg
    23521 gttgattctt tcaatctgtt gcagtgattg caaacacata tccaggtcag tcaccagatc
    23581 ttcccggctg gggggcgatt ttttaatggt tttacgtgat ttgtaatatt tttgcagcct
    23641 tttgtcaagg gcaggtgttt tgtttaaatt gaagagcacg gcaaagtcaa ccgaggtcaa
    23701 aggctcgttg ctcatggtac ttagcttgaa aaaataaata cgggtattgg taggaccata
    23761 atagtacgga ccaatttcat gcaaatactc attctcctgc aaaatggcca gatcttcccg
    23821 gctgttgagc tccgtctcca acaattccat ccactgatta aggcaggatt tgggggaatc
    23881 ctccccttga ctccactcct tcatttgatg atatgtttca agatggatgc gaatgaagga
    23941 attataatag taataaagaa ttttctccgg gctgcagttg atgaatactg ataaatcgag
    24001 gaaatggcaa tcctccttgc ttagaaatgt tgataacatg gtccatcctc cttaaattaa
    24061 aataccaata aaggatttct atatattacc aataatatcc tttaaatagc aatggaaaaa
    24121 taccttggtt tttcgccata tcactgatat cgtggctgtc tttttcccgt cggaatttgc
    24181 tataatcttt aaaacagttg ataaaaagag gagaataaca tgtattcagc aatcctgttt
    24241 gatctggatg gtaccctttt acatattgat atggaagaat ttttacaata ttattttgct
    24301 gagatgatgc gtgctgccag ggaagcgggt tacaaagaag gccaaaaact ggttgagcag
    24361 gtgtttgcct caaccagcct gatgcttaat gatttttcac cgacagtttc caatgaagcc
    24421 gtttttatgg gtgacttcct gtcccattgt gattatcctg aagaggaagt gcgcacgttt
    24481 tttgatcatt tttatcagca tcattttccc cggttaaaag ttcattgccg cccttttcca
    24541 gggataccgg ggatgatgca ggaacttttt caacgcggtc ttaaggtggt cattgccacc
    24601 aacccggttt ttccccggga agccattcag caacgcctgc aatgggctgg gattgatgac
    24661 ttgccttatg cattggtgac ttcctatgaa gagatgcact tctgcaaacc ccaacttgcg
    24721 tattatgaag agatttgtca gcggattggt gttggagcag acgagtgtct gatggtgggc
    24781 aatgatgtgg atgaggatct tcctgccggt ttgctcggaa tgaaaaccta tctggtggag
    24841 gatttgctga tagacaaggg ccaaagcaga ctttttccag actggcgtgg aagcgtaaat
    24901 gacatgatcg cttttctgaa aaaaaccggc tcataaaaaa ttgcctctcg ggacattata
    24961 tggtcagtac ttgacgaccg ggagagatgt gaaacatgag cagaaaaaag cccaaggcca
    25021 atcggccatt aacggaaaga gatttgctta aattagaaat tgcccgtgaa atgggcgtcc
    25081 tggagcaggt ggaaaaggag ggctggcaat cgatcagcaa tgcaacctgc ggacgtttgg
    25141 gggcgattat gcggcaaaga ttaaagctga ccgacagaaa tacggaataa agtttggttc
    25201 aatgcatgga gccgtaaagt catttaatag aataagcatg tggtttgatt tataattaag
    25261 tcataaatca aaccacattt tttatataag aggatgacaa gatgaacgaa atcgaacgag
    25321 tgagaagcta tttgcaggaa aaaaatccac gtctgcagat cattgaactg gaccaaaaca
    25381 caagcaccgc tccgctggcc gcagcagcac tgggcttgga agtcggacaa attgccaaat
    25441 ccattctatt taagaccaaa agtgagcaat atttgatggt ggtatcagcc ggagatgtta
    25501 aaatggacag caaggccatc aaagagactg tcggcgagag agtgagaatg gcgaatcccg
    25561 aggaagtata tcatgtgacc ggtttttctg tgggaggagt gtgtcccttt gcactagccc
    25621 atgaagtacc catctatttg gacgaaagcc tgctgcgcta cgatgtggtc tatgctgcag
    25681 caggtacggc caatacagcg ctgccgataa catttactga attacaggca atcaccgccg
    25741 gccggccctg taaagtttcc ctatgagaat tactattttg ctggaccagg agaagaaagc
    25801 atgagcaaag gctttgtaca cttacataat cattctgaat acagccttct ggatggtgcc
    25861 tgccgggtca aggaactggt cagtcaagcg gtccagctgg gtatgccggc agttgccata
    25921 actgatcatg gcgttctcta tggggtcatt gatttttatc gagaggcaaa aaaacagggc
    25981 attaaaccga tcattggctg tgaagtttat gtagcgcccc gcagccgttt ggacaaggaa
    26041 gtccgcattg atgacaacca gtatcatctc attttgctgt gtaaaaatga aaccggttac
    26101 aagaatctgg ttaaaatggt cagccgcgcc ttcattgagg gattttatta taaaccccgc
    26161 gtcgaccggg aactactgga gcagcatcat gaagggctga ttgccctttc cgcctgcgta
    26221 gccggggaga ttccgcagat gattttaacc gggcaggggg aaaccgcccg ggaaacagct
    26281 ctatggtatc agcagatctt tggcgcaggt aatttttatc tggaaatgca ggatcatggc
    26341 atggatgcgg aaaaaatcgt ctgccgggag ctggccagga ttagccagtc cactgggata
    26401 ccgctggtgg caaccaacga tctgcactat atcagacagg aggatgccgc catccatgac
    26461 gtacttttat gcatccagac tggaaaaacc gtacaggatg aggagcggat gcgctttccc
    26521 ggcaatgatt tttatatgaa aagtgaagcg gaaatgcgat cgttgttcgc ggactttcca
    26581 caagcgctgg acaatagtct gctcattgcc gaggcctgca atttggaatt tcactttgga
    26641 gagtttcatt taccttattt cgatatcccg gcgggctatg acccggaaac gtatcttgag
    26701 caactggctt gggaaaaatt tgcccaacgt tacccgcaac caacagaaaa aatcatcgaa
    26761 cggttcaatt atgaattgca gatgatcaaa cagatgggat ttgcggagta ttttttgatt
    26821 gttcaggatc tggtaaattg ggcccggtac aaccaagttc cggttggacc cgggcggggt
    26881 tcggcagcag gtagcctggt ttcctatgcg ttggggatca ccaatattga tccgcttaaa
    26941 tatgatctgc tgtttgaacg ttttcttaat ccggaaagag tcagtatgcc ggacattgat
    27001 attgattttt gctttgaaaa acgcgaccag gtaatcaatt atattgtgga gcgctatggg
    27061 gctgatcggg tagcgcagat cattacattc ggaaccatgg ccgcccgtgc agccatccgt
    27121 gatgtaggtc gggccttgaa cgtaccgtac ggggaagtag acaaaatcgc caaattaatc
    27181 ccggctgagc tgggggttac cttggatcgt tcgctggaaa ttgcccctga tttgattgaa
    27241 ctgtatcaga acaactatga cgcccgtaaa attatcgatc tggccagagc cattgagggc
    27301 atgccgcggc atgcttccat tcatgcggca ggagtggtga ttggcaaaga agacttaagc
    27361 tccatcgttc cgctgcagaa gactgcagat ggacatgtca ttacgcaatt tgccaaggag
    27421 acggtggaag atattggctt gctgaagatg gatatattgg ggctcagaac cttgaccgtg
    27481 atcgaccggg ccttggcgat tatcaaaaag acccgtaatc ttgacataag cattgatgag
    27541 cttccgttgg atgcgctgga agtatacaaa ctattgcagg aaaaaaatac gattggcgtc
    27601 ttccagttgg aaagtgacgg actgcgcaga atcctgggag aaatgcagcc cagccgtttc
    27661 gaagatctga ttgccatcat cgctctttat cgtcccgggc cgctgggcag cggcatggtg
    27721 gaagatttta tcaattgcaa acacggccgc cagaagattg aatacatcga tccccgtttg
    27781 caggatatcc tccaggaaac ttatggggtt gtcctgtatc aggaacaggt catgaggatc
    27841 gcttcggatc tggctggttt tagcatgggc gaagcagatg tcctgcggcg ggccatgggc
    27901 aagaaaaagg tcaaggaatt gatggaccag cgtgacaagt ttgtggacgg tgcagcccgg
    27961 aaccatattt cggcagagaa cgccacccgt ctgtttgaca ttatggagag ctttgccggt
    28021 tacggcttta ataaaagtca ttctgctgcc tatgccctga ttacatacca aaccgcttat
    28081 ctgaaagctt atttcccgga agaatacatg tgtgccttcc tgagcagcgt aatcgaccat
    28141 caagatcggg tggttttcta catcaaagag tgcaaaaaaa tggggatcga catcctgcca
    28201 ccggatatta acgagagttt cgagaatttc accgtcagcg ccggcaggat cagatttgga
    28261 ctgggagcaa tcaaaaatgt gggcatcaat gcggtcaagt ctattgtcca gactcgcaaa
    28321 caaggcagct ttacctcctt gtttgacttc tgtcagcgga ttgatctgtc tcaaatcaac
    28381 aaacgtatga tggagaacct gatcgtggcc ggatgctttg atgcattaca tatcacccgt
    28441 aaacaagcct tgtcgattat ggatgaatgc atggaactgg ccagtcagat caaactgaat
    28501 gaaaactctc agcagatatc tttgtttggt gacagcagcg aattgattgt tgaagagccc
    28561 cgcagcaagg tgaaagggga gatggatgtc cgtgaattgc tgagccggga aaaagaagta
    28621 ctgggctttt atgtatcaga aaatccgttg gacagattta aagatatcat tcccctgatt
    28681 gtcagtgaag aattggggga gcttgagaag gacacgcatg aaggctatgt cagagtggcg
    28741 ggcctggcca gtgaactcaa caaaaaagtg agccgcaaag gtgaagccta tgcccgtttt
    28801 gtcctggaag acctgagcgg ccggatggaa atgatgatct ttccttcggc ctatcaaaag
    28861 accatcaata aaatcgaacc tgatcaattg ctggtggtgg agggattcct cgatcgacgg
    28921 gatgaaatgc ccaaaatagc tgtacgcaat gtcggtgtac cgggcaaaga agtgaaagat
    28981 ctgcatatcc gattgcctta tgaaaaggat gatgatcaag gcagacagga attgctgggg
    29041 aaattggccc aatatccggg caatattgag gtggttctgc acctgcccaa taaaaaaatt
    29101 gtggttttga cggaaaaatt taatgttgcg gcgaatctgc agttaaaaaa cgatctaacc
    29161 cgtttgtatg gaaagggcag catctggtac agttgataac gtatcatctc tcgcacgaaa
    29221 atgaatgaac cggaacggtt tggcgaagac tatactgggg aaaacaagca accaggcaat
    29281 aatgactgat tccggaatac aggtgagcgg aacaactgga gggataagta tgcataaagt
    29341 ggcggtttta agcagcggcg gcgatgcttc ggggatgaat gcggcgatcc gggcggtcgt
    29401 gagaaccgcg ctgtatatgc gtatgagagt ctatggggtc cgcagaggtt ttgcgggttt
    29461 gattgaagat gacatggtgg agatgaatgc cggttcagtc gcggacatca ttcaacgtgg
    29521 cgggaccatc ctgcagacgt cccgatcgga actctttaaa acagacgagg ggcggcagaa
    29581 agccagccaa aacctactta accgggggat tgaaaacctg gtggtcattg gcggggaagg
    29641 cagtttgaaa ggagcctatc atatctctcg gctgggaatt aatgccattg gtattccggc
    29701 aacgattgac aatgatattg tttttaccaa atcaatcggt tttgatacgg ctgtcaatac
    29761 cgtgctggat gccatcaatc gcattcgcga tacagccagc agtcatggac gcaactttat
    29821 catcgaagta atgggtcggg aatgtggtga aatcgcgttg gcagctggcg tcgccggagg
    29881 cgcggagtca attttgatcc ccgaaatcgc aaccgacctg gacgcagtca ttgataaaat
    29941 taatcagggc agcaaacgcg gcaagctgca cagtattatc gttgtagctg aaggagttta
    30001 ccctccgctg gagctgagaa atattatcga aagacggacg gggcatgata cccgcatcac
    30061 cattctgggt catactcaac gcggtggttc accgactgcc caggatcgga ttttggcttc
    30121 ctgtatgggc aaggctgccg tagaattgat cgcctcccaa aatcgcaacc aaatggttgc
    30181 cgcggacggc aagcattttt ttgccctgcc gctgcaggaa gtgataaacg ggaataaaac
    30241 ccttgatttg gaaatgtttg caattgcccg gatgctttct atatagacag gagtgttatc
    30301 atgcgcaaga ctaagataat atgtacgatc gggccggcca gtgaagatgc agagcagcta
    30361 aaaaaaatga tcatcaatgg catgaatgta gcccggctta atttttccca tggaacctat
    30421 gcagaacatg aaatacggat tgaacggtta agacaggcag cacgggaact ggatcgtccg
    30481 ctggccatca tgttggacac caaggggccg gaaatcagga ccggcaaatt gaaagacgga
    30541 aaagtaaact tgcattctgg acagccgttt gtactgacca accgggaagt aagcggtgat
    30601 gaaaaggaag tgcaagtcag ctatgcgccg ctggcgcaag aggttggcaa aggcagtacc
    30661 atcctgctgg cagatgggct gatccattta caggttgaag attgccggga tgaagatgtc
    30721 atgtgcacgg tggtaaatgg cggtgtattg ggagagaaaa aaggtgtcaa tgttcccggg
    30781 gtgagaatca atatgccttt tttaagcccg aaggacgttg ccgatatcca ttttggcatt
    30841 gatcatgggg tcgattttat tgccgcatcc tttgtcagga gcgcggaaga tgttctggaa
    30901 atcagacgga ttctggagga gcggaatgct gacattgata ttgtagccaa aattgaaagt
    30961 cagggcgcgg tagacaatct cgaagatatc atcaaggtcg ccgatggagt catggtggcc
    31021 cgcggtgatt tgggggttga gattccggct gaggatgtac cgctggtgca aagaaaaatc
    31081 gtggcgcaat gcaattatgc cggcaagatt gtcattattg ccacgcaaat gctggagtcc
    31141 atgattaata atccccgtcc gaccagagcg gaagtatccg acgttgccaa tgccatattt
    31201 gatggagcag acgccatcat gctatccggg gagtcagccg ctggtcatta tcccctggca
    31261 gcggtggaaa ccatggcgcg gatcgccaaa cgagctgaag aagcgattga ttataaagaa
    31321 attctggcga aaaaaggacc ccaaggcaaa ctgtccgtta ctgacgcgat cagctatgca
    31381 tgctgtacta ctgccagcaa tctgcagatc gcatcgatta ttacgcctac ccaatccggt
    31441 ttaacagctc gcagtattgc caaatacaga ccgcaggctg atatttttgc cgttaccagc
    31501 cgggaaaagg tcttgcggaa attaaccctg gtctggggcg ttcatccggt ccatgcccgg
    31561 aaaaccaatg gcacagatga attgctggat gaagcgctga actgctgttt ggacaaacaa
    31621 tatatcaagc gcggagacat ggtggttttg agtgcgggca gtccaaccgg catttccggc
    31681 gggacgaatc ttatcaaggt gcatattgtg ggagaaatat tggcctatgg catgggtatc
    31741 ggcaaccatc cggtcagcgg tcctgtcagg ctgatccttc atgaggaaga tctgtctaaa
    31801 attcagtccg gggatattgt ggtcacccat gccgccagtg caattttatc gtcctatttg
    31861 gacaaaatca aggcgattat tgctgaagcg ggggggttga cttccgatgc tgccatcatt
    31921 ggtttaaatg caggcattcc ggtgattgtc ggctgcgatg aggccacccg tatttttaaa
    31981 gatgacatgc tggttaccgt ggacacagca catgggagaa tctttaacgg actgaccaag
    32041 gtattataac aagttacata tttaagggga gttaggtttg ttcaacatta atcaacacat
    32101 tgcagtggtc gagtttcatg gttttgaata cccccattgc aactgcctgc tggtccggga
    32161 tgacatcaat tgtctgctgg acaacagccc tggaagggct gatttggaaa acctgtgtcg
    32221 ccagccggtt gatattatag tcaattcaca tgggcacatt gatcattact tatgcaacca
    32281 gtattttcct agcagcaggg tctacatgca tccggccgat caggctatcg cccagtcagc
    32341 cgaaaaatac ctgcaagcct ttgggttgac cacgttaacg cataacccgt tgttgcaaca
    32401 attatatctg aaggcagtca aatatcggac cgcaatcata gatgaagata tcagcgaagg
    32461 tcaaaagttg aattgcggcg ctaccaaaat cgaagttctg catttgcccg gacactcgcc
    32521 cggtcattgt ggatttttat ttccggatca gggcttcatc tttactgcgg atatagatgt
    32581 ttccaatttc ggcccttggt atgccaatct caattcttcc attattgact atgtacagtc
    32641 gatcaatcgg gtcatcgatt tgaagcctga ctttatcatt tccggtcatg gtcaggcgat
    32701 gattaaagac ggcttgatga aaaaactgac cgattatcgc gacattatct ttgcccggca
    32761 ggagcgcatc gtggaattgc tttttagagg gtatcaccgc cttccggatt tggcccggca
    32821 atgcccgatt tatatcaagt tcccccgtcc ccgggaaata ttctatatct acgaacaggt
    32881 catgatcctc gcccatttgc gatatatgga ggaaattggc caggtaatca gggacggaga
    32941 ccaatattac ttaaaagacg gaattcgtca tagcaaatca tatccgcttt aatgcttgtc
    33001 tggaggatgg ctaaaattaa tgtccaacca tcgttttaca gctcaacaaa caaggggctt
    33061 ccattaggag gaagcccctt gaacatttcg ttgattgccg ggtaaggatt tgcctgcaga
    33121 ctatttgccg ctgtaatcat agaagccacg accggttttt cttcccagat aaccggcacg
    33181 caccatttgc ttcaggatgg gagcgggacg atatttggag tcacctgtct cattaaacaa
    33241 ggtgttgcat actgccaggg atatatcgat gccaaccatg tcagccaggg taaagggacc
    33301 catcggccag ccggctccca atttcatggc tttgtcaata tcttcaaccg ttgccagtcc
    33361 ttcatagaga atgaaagctg cttcgttcat gccaggaatt aaaatccggt tgacgacaaa
    33421 ccccggacct tcttttactt ttatgggttt tttgccaatg gccttgctga gttccatggt
    33481 aacggccagc gtttcttccg aagtctgcag ggctggaatt acttcaatca aagccataat
    33541 gttggcagga ttgaagaaat gcatgccgat gaccttgtct gaccttttgg taatggcggc
    33601 gatttcagta atggaaagcg aggatgtatt tgtggcgatt atgatttccg gcggcaatat
    33661 ctgatccaat tgtgcataga ggttcttttt aacctccata ttttcaaata cagattcaac
    33721 cacatagtct acatctttga tgtcttctat ggtgccggtg ggggtgatgt tgccggtcac
    33781 aaaatctttg gcgccagcgg ggacttgtcc tttttcttct gctttggcca gtcctttgcc
    33841 gattctttcc agggctttat cggagaattc cttttttatg tcgtacagca cgaccttttt
    33901 ccctgcgccg gcaaatgccc aagcgattcc cattcccatt gtacctgcac ccagaacagc
    33961 tactttatta acatccatta tgtgccctcc tcatttttaa cagtgctaat taagagttta
    34021 aacgaaatag aattattttt caatatttta acagtttaaa atttttaatt aatgataaat
    34081 tgagacagcc aaaaccgtaa ccgcaaagga aaagatttcg acaaaatatt actcgctttg
    34141 agattcatgg tggtctttga ttaaactgac gactgtcgaa ttgaaaacgg cgaacaccgc
    34201 atcaccttcc tgcaggtcca tgtcgctgta tgcgctcgca gtaatggttg cggtgacggc
    34261 ttgatctccg acgtcaacga tgatttccgc catatcttgc ccagtatgga ccttcatgat
    34321 tttaccaaac agtttattcg gagcacttat tcgcatacta ttcctccttt ttaatatagt
    34381 ttgcacggat gccagtcaaa atattttgct ttcaagtaca ggcataaaaa atcctttgat
    34441 gccatcaaaa acccctgtca gggtcgcatc tgacaggggt tgggtatatg atttttaaat
    34501 tgttggctta gttttaccag gtatcaacga agggaagcag ggattctcca tctttgcgcc
    34561 gcggggctga ttttaaacct cgcataatcc aggtgcgggt atcagcggga tcaatgacgg
    34621 catcaatttc aaggtaggat gccatattga ctgctttacc tcgttcatac agcttgtcga
    34681 ccaacttgtt gtaaagtgct tcacgttcct gttcgtcctc aatggctgcc agttcccttt
    34741 tgaaaccgct tttgacggct ccttcgagtc ccatagcacc aaattcaccg gtcacccagg
    34801 cagcagtgaa aaacgactcg tggaagccgc ctttggccat ggccatggct cccaaaccat
    34861 atcccctgcg cagagcaatg ctgaaataag gcaccgtcac atgagatcca acgacaaaca
    34921 tgcggcaaac atgacgaacc tgggctcttt gttcaacatc aggtccaacc atgaaaccgg
    34981 gggtgtcaat caaagataaa attggcagat tgtgtacatt acatagctgc attaaacgag
    35041 c
//
LOCUS       DAOTHZ010000015        34174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1403_length_34174_cov_8.372989, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000015 DAOTHZ010000000
VERSION     DAOTHZ010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 34174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34174
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_34174_cov_8.372989"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="P5549_03235"
     CDS             <1..744
                     /locus_tag="P5549_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRW12119.1"
                     /translation="VTQMVDMMTPIVAPVLAFYLEGGAKTILVDTGVVEPGPDGTFHG
                     FPLIGGGAQGMINALAEIGLKPEDIDIVIITHMHFDHAANISLFSNAQFILQKKEWEY
                     AKAPLPIQKGVYLTDLLTELEEYDLVLADDGYEVADGVNVICIPGHTNGQQAVVVNTS
                     DGTYVLSGDLMYSMANLYPQTTEIIDSLGNKVPCTPQLGHPFYPPGIHTNLNDWYSSA
                     WRLLSIAGTRKHILPGHDPSLVGRVFPEK"
     gene            790..2415
                     /locus_tag="P5549_03240"
     CDS             790..2415
                     /locus_tag="P5549_03240"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRW12120.1"
                     /translation="MTATKLEFPTTRLEDLPYLQGRQLNFDSIAEMFYTRVNEEPDAI
                     HVLYYDQVITYAQTNQRANQVAHYLKGKGVQKGDVVSIMVLNSPENYYTMFGAQKLGA
                     VAGAVNYMLKGPEIAYLLDDCQPKVAFVSSEFMKDFAAGYALAQHKPVVVEVVTGVEH
                     DSHINECTLADILSQYPVTETLVKQNPDDPFMLLYSSGTTGNPKGILLSNKGQLSICR
                     DMNRIRLFQGKDVMMIILPMFHTNPLCVWTYPLTYGGHTICIRKTFSPLDFWPAVMQY
                     GVTVVFGVPTMFNYIYNAADPKIINPAKLKIRYAFSGAAPLPVELIHGFKEKFNIEIV
                     EGYGLTEVSGVSTVNPPAGKKIPGSVGLPYPEQEIALMDENNQIMPPGEPGEICVRSD
                     ANMLAYLNQPDATAETIKDGWLHTGDVGRLDEYGYLYIVDRKKDMINRGGENIYPREI
                     EIAIEANTKVSEVAVIGVPDPALGEKVKAFIITRPNQSLSVDEIKEFLQDKIAKYKIP
                     EFIEITNDIPRNPTGKILKKELREMEAAKTMKE"
     gene            2633..3982
                     /locus_tag="P5549_03245"
     CDS             2633..3982
                     /locus_tag="P5549_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12121.1"
                     /translation="MNYSKDYSSKLVTAEEAVKVVKSGDRIFYSEFAMFPKMLDAALA
                     KRIPELHDLELYSVFYTQVPKAIEADPQHEHIIIQDWHFGPVSRHLAEEDKCFYVPIA
                     YHQGPRIIHKYIENDVAMVAVSPMDEKGFFNLGPTNSVTPAYLDRSKIIILEVNNSVP
                     HCLGGNSENIHINDVDYVVEGNNYSLIQLPAAKPKPFDCQIAGYIMNEIEDGACLQMG
                     IGSMPNVVGANIADSDLKDLGIHTEMLVDSCVDLYESGRVTGNRKSIDKGKMVYSFAL
                     GTDKLYDFMNNNPVCASYPVNYTNDPRMIALNDKVIAINSALEVDLLSQVSSESSGYR
                     HISGTGGQLDFIMGAFQSHGGKGFICLSSTYEDKHGQLHSRIVPSLRVGSVVTVPRSM
                     IQYVVTEYGIVQLKGKSTWQRAEALISIAHPDFQDELVEQAQKMKIWARSNKNEEKP"
     gene            4223..4612
                     /locus_tag="P5549_03250"
     CDS             4223..4612
                     /locus_tag="P5549_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HRW12122.1"
                     /translation="MQYKAPSQCPVCGQELIISKLSCKQCQTSIEGLFSPCKFCRLPK
                     DQLDFIETFIKCRGNIKDVERELNISYPTVRKNLDTVIQSLGYQLERSEEREEEIVLR
                     RKEILSALERGEINSKEAAKKLKEYKR"
     gene            4667..5089
                     /locus_tag="P5549_03255"
     CDS             4667..5089
                     /locus_tag="P5549_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12123.1"
                     /translation="MNQETMKILELLEQGKITRQEAMELINSLETRSDSEGGAPVSGE
                     NTKRFLRVRVNGDKTKVNVNIPVNLIKVATQIADASMKWIPQEAHEQIKRQGINLSQI
                     DFDEIIKLVDQGLSDGRLVDVETEDEKEGRIKVEVYVE"
     gene            5109..5393
                     /locus_tag="P5549_03260"
     CDS             5109..5393
                     /locus_tag="P5549_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12124.1"
                     /translation="MRIQAGQKPGLYIPLPLVVLDVTLASLHECLEFWQVLFLNLVVR
                     KIGLDEIKNRPKPAEILALLIKMIDEIRSYGSFELVWMEDEENKISLRLY"
     gene            5430..5771
                     /locus_tag="P5549_03265"
     CDS             5430..5771
                     /locus_tag="P5549_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRW12125.1"
                     /translation="MKRFIWKSIVNICVIYSLSLFLPQVFLQGAFPAILAGALLSLLS
                     VTLRPLLMLLCLPLNLLTLGLFVLVINTWMLQLTDLMVKGFVVSGFWYALLAALFIML
                     ANKLVNVLRYN"
     gene            complement(5810..5980)
                     /locus_tag="P5549_03270"
     CDS             complement(5810..5980)
                     /locus_tag="P5549_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type small acid-soluble spore protein"
                     /protein_id="HRW12126.1"
                     /translation="MDIKRANEIFDSLGVIEVFYQGSPVWIEKVAANRIQVQDLRTKQ
                     RFEVSVYNLSEE"
     gene            complement(6204..7445)
                     /locus_tag="P5549_03275"
     CDS             complement(6204..7445)
                     /locus_tag="P5549_03275"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRW12127.1"
                     /translation="MAESEYKVVRRLEGQKRLAGMIMAHYGGALEIEKNKSKPVAWVS
                     AGTPAEILWAMDFCVQFPEAYAATCGARHIAHSHVEITEQRGYEHHLCTYCRNSLGAS
                     ISQLEGMSQFSELARPDFLLVANNSCILITKWWEHLSQLWNIPMFNIDCPLVIPAYEE
                     KYLVDHVKRQCLDLITYLEEFTGKKFDYDRLQEIVANGQRSSKYYMAMLEANKHDPVP
                     ATFFDIMGHNFPNLVLRYLPETAEHYRLMTEELNQRIADKIIPMSNMKHRIYWDGIPY
                     WFAIRELSEKLESLGLCLVTSGYFDMFSFEQLDSSRPLDSVAEGVATMILNHNVSFKT
                     KITENIFRNYNLEGGIFAYAQSCKPFSITMRYIAEHVQKSLDVPVTIIEGDLVDETFY
                     DGERNNMKLQALAETLAARRG"
     gene            complement(7532..8311)
                     /locus_tag="P5549_03280"
     CDS             complement(7532..8311)
                     /locus_tag="P5549_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRW12128.1"
                     /translation="MITAGIDIGSTTTKAVVLKADDQFVYALQPTGIEISSVSRQVLA
                     EACENAGLQPEDLDRIVATGYGRISIPFATTTITEITCNAYGVHYLFPNASLVVDIGG
                     QDSKVIKFDKNGRVVQFAMNDKCAAGTGKFLEVAAQTLGVPVSEMGDISQQSQNKVSI
                     SSTCTVFAQTEIVSLIARQTNKEDIAAGLHESIVSRVFGLISSVNPDPQAEVVLTGGV
                     AKNVAIVNLLERMLGRKISVPEDPQIVTALGAALLARQWES"
     gene            complement(8327..9472)
                     /locus_tag="P5549_03285"
     CDS             complement(8327..9472)
                     /locus_tag="P5549_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRW12129.1"
                     /translation="MEEILATFRDYCEKPYERAAQIKAAGKTKIIGCLPMYFPEEIIH
                     AAGALPVTLFGSDDPITLGDGHLMTNACDQVRSSFDSLLKGKYDFLDGIAAIYICDQV
                     RFYLEVWQLDHPVDFFHQMWRPYKWDSSTQEFFHRELKRLIARLEEFTGNKITTEKLK
                     ASIRLYNKSRNLMRQLNELRKEHPGIIRASDMACVVASSMLLPKEEHNELLAKLLAQL
                     TQQELSAGKKQRIIAVGHPCSMPEREILDMIEDNGMLIVDDDFFTGGRYFSKDIALND
                     DPLDAIIDFNANAIPCTTGHYADAWIQPDKIYSPYAEYVIDLMKHGQAKGVVVLKEMY
                     CDPYDMEFVLMKNRFEEEGIPYISISSGSLEAIRTRVQAFGETLQFV"
     gene            complement(9760..11451)
                     /locus_tag="P5549_03290"
     CDS             complement(9760..11451)
                     /locus_tag="P5549_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12130.1"
                     /translation="MTGLTPAMMEQRFWLKSYDNITRPTLNYPKFPAHEILHMIATTN
                     PDKAATDFYGTEITYWDLFISSTRMANGLIEAGIKKGDRVGILLPNCPQMIVSFWAIL
                     QAGAVIVNLNPMYKPEELKSMIEKTGMTALITFDGLIAGVQQLCQMVNISFVMVTKLT
                     DYINEMKVSTPEELGLPEGWHHFSSFLAGSANTAPPSLAFNVEEDAAVLQFTGGTTGT
                     PKAATLTHRNLVAALYTGNEWGRSVSEFIPIERRNIMGVLPCFHVFGEICGIAMSAIT
                     VSTLIILPRFDLDEFMNTMERIPHVWFFPAVATMLGAIINHPRVKEINLAKKIAVINA
                     GAAPCSLDLLKKARELGFIISEGWGMSETTSVGITTPFLGVKKPLSIGIPYPDAEVKF
                     VDVNTGEEVPRGKPGEMIMKSPMVMKGYWNDPEETAQQLKDGWLHTGDIAYQDEDGYI
                     FIVDRAKDLIIAGGYNIYPQEVDQLLLQHPQVADAMTIGIPDEYRGETVKSFVQLIES
                     ATVKENELIAYCRDHLAAYKVPRSIEVRASLPRSATGKAMKRILRDEELAKMKKS"
     gene            complement(12104..13474)
                     /locus_tag="P5549_03295"
     CDS             complement(12104..13474)
                     /locus_tag="P5549_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRW12131.1"
                     /translation="MAKWKSISTKILIGFLSVVLFTFLVTALAFYPILVGVLEKRAEI
                     GLEKQAWEISHAIGSGTVPSEDNKTPLTILLLGRSVESDFLWISPQDQIIYSSWPEKF
                     TPGQRIFQLSLRQEEPFDRSRVNVFKNDFYLTVEVPASNGGTVMTFVALSKLQSMYRE
                     TLATIFRSLAVALAMALLIAFFLIRYLVKPLRSLENYAKAVGNRQFDVRLELKSDDEL
                     SKLAIALNDMAERLQRYDESRSRFFQNASHELKTPLMSINGYAEGIRDGIFSGPSLDH
                     AIDIIHKESQRLRDIVENMIDITILDQPHETYLLPHDLDYIMDQALETVGGYALERGI
                     QIKCEISPAIGVIGDWDQLHSLFVNVLSNAIRHANHNITVTAALGNHNTQVTIKITDD
                     GAGFTKTDLENAFDYFYTGDKKGSGLGLTIVKKIVEEHAGAIKLCNAPTSGGVVQITL
                     PSSPES"
     gene            complement(13446..>13856)
                     /locus_tag="P5549_03300"
     CDS             complement(13446..>13856)
                     /locus_tag="P5549_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW12132.1"
                     /translation="DDYLPKPFSPRELVARVRSVFRRTMVPPDPQPYMLAGNLKLVTQ
                     DRLVLIDGEEISLSSKEYDLLALLIQHPQRTFNRQELLDRIWGYDYEGSERAVDDLIK
                     RLRRKLNESNSKTNIKTIWGYGYRLDGKVEKHIY"
     assembly_gap    13857..13956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13957..14296)
                     /locus_tag="P5549_03305"
     CDS             complement(<13957..14296)
                     /locus_tag="P5549_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW12133.1"
                     /translation="MNTIFVVDDEENIRQLIMQYLQNEGYVVEAFGNVEDLSRRLETS
                     YPDMLILDIMLPGQDGLQFCRDFRQHSRIPIIFISARRKALDRIAGLEIGGDDYLPKP
                     FSPRELVARVR"
     regulatory      14620..14747
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            14865..16076
                     /locus_tag="P5549_03310"
     CDS             14865..16076
                     /locus_tag="P5549_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HRW12134.1"
                     /translation="MEQISEKIIVKDLIKIFESHTQKELNLLKTGKSKEEMRRETGLT
                     VGVNNISFTVKQGEIFVVMGLSGSGKSTILRCLNRLIEPSAGEIIIEGTDITQLKSKE
                     LQSFRQKKTAMVFQQFALLPHRKVLQNTVYGLEVQGVAKKEREEKAMQALELVGLKGW
                     EDKYPAQLSGGMKQRVGLARALTNDADILLMDEAFSALDPLIREEMQDELLNLQQKMN
                     KTIVFITHDLNEALKLGDRIAFLRDGAMVQVGTPEEITTSPADDYVARFVRGVDMSKI
                     LTAGDVMKKPQPLITLRDGPHVALRIMREQGISSVFVVDRNRHLQGIITVDMAVEAAR
                     SGIISLIDVQLAEGPVVQRDTPVLDTLGIIAESKLPMAVVDDENRLIGIIVRGAVLAA
                     LASYKGGEENE"
     gene            16069..16923
                     /locus_tag="P5549_03315"
     CDS             16069..16923
                     /locus_tag="P5549_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRW12135.1"
                     /translation="MNKFPLDALIDQAVKWLQINYGESVDAFSAAFSESINFMGDSLA
                     MVPWWLIILVFAVIAWRCNGWKLSLGTVIGLYIIYNLRLWAPFLDTLILVIISAALSI
                     IIGIPLGIMAAKSNKFHKGISPVLDVMQTMPSFVYLIPALLFFGIGKVPAVFSTVIFA
                     MPPAIRFTDLGIRQVPTDLVEVGEAFGSSPGQLLWKIQMPVALPTIMAGINQTVMLSL
                     SMVVISAMIGAGGLGAGVLEAISQLKIGMGFEYGIAVVIIAIILDRISQGIGRSLGTV
                     DNKNNKEE"
     gene            16926..17816
                     /locus_tag="P5549_03320"
     CDS             16926..17816
                     /locus_tag="P5549_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRW12136.1"
                     /translation="MLKGQKRKYKILIVALIVALTAGLVVGCSNSQQPSADKDKGTIN
                     IGYVNWAECVATSNLWKVILEEQGYTVKLTQLDVAPVFVGLSKGDIDFFMDAWLPITH
                     QTYWEEYKDNLEDYGIWYEAPAKIGLAVPQYVTIDSIAQLKDEAGKFNGQIIGIDPGA
                     GIMKASERANEEYQLNFEVVQGSEAAMMTTLEKAYGKQGWIAITGWSPHWMFAKYDLK
                     YLEDPKKSFGEEEEIHILANKDFSSKQPDIAAMLKKFRMDDAQIGSLEALINEGMQPI
                     DAAKQWKNDNQDIVDSWTKS"
     gene            18230..19537
                     /locus_tag="P5549_03325"
     CDS             18230..19537
                     /locus_tag="P5549_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12137.1"
                     /translation="MPFPKNEIVMELSLHPENLPVVMNMAEQAALVFGMQSKDTMALR
                     LAAEEVFSYLCTHTDMQDTIRLECKKGIYYIQMDFIFQHSNLDLRAFNISTDINLEDD
                     SQLEEMGLLIAARSIDRLFLNDEQNRMVLSLYKEKSYPPAEISTPMSRNTTEFFIKPA
                     GIEDLKSFCQLAASFDKDTQELPVFLRYPGKFADMIYSGEYQAALAFDQQQAILGGIV
                     WKSQAQRTIEFYGPYLFTRFDGMAEALIESCLEQIGRSDAVGLVNRCGTVDLSEKYFE
                     KLGTIQYLHNDDKVLDITAYYRQLCEDPGQRIWTHPDLLPYLQSQYQRLFLPREIRPV
                     SNMGEYKHAHSVFSSEIGHDQVILRPLLGGEDTAANLKGHLQLFAREKIPNIFFEIDL
                     GLAWHAELVPALLCQGFKPGLILPYAGKADLLLMQYRAGDVTC"
     gene            19531..20634
                     /gene="hisC"
                     /locus_tag="P5549_03330"
     CDS             19531..20634
                     /gene="hisC"
                     /locus_tag="P5549_03330"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRW12138.1"
                     /translation="MLNVALDHLVPSYINNFDAYIPSKPDPELKKMFGCDTLYRLNNN
                     ENPLGTPPAAQELFQKFNPARAAIYPSGDSYYLREKLADKFSLHPDQILVGNGANEVI
                     AFVINAFCESGDNIITADKTFATYEWIATFSGYEARLVPLKEYAFDDQAMLEQVDARS
                     KIIFICNPNNPTGTYWNQEKLVRFLDQVDGQQIVVVDEAYCEFVEKEDFPDTLSLLEK
                     YPNLIIFRTFSKMYGLAGLRIGYLIGNLEVVNIIRKTCVVYSVNTIAQEAALLALDDQ
                     EHVIRTRKLVAEEKAYLRQEFDRLGLSYVINEGNYVMLKLPISDTLAYRKLMREGVMV
                     RTMTGFRFPNYIRVTIAQHEAMEAFIRGLEKIL"
     gene            20691..21713
                     /locus_tag="P5549_03335"
     CDS             20691..21713
                     /locus_tag="P5549_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12139.1"
                     /translation="MKNSTKNDLELDIRLMLPEDAPGVVELYRSVYGDEYPIKSVYDV
                     EGIIRQQNNGDMIRTIGVSNKGQVLGQTAIYRSCVSNPALYEEGQAIVLREYRNQGIL
                     ERCHDYAHNQVYPQIMEQLWGEAVCNHVFTQKSSIRAGGYETGIELDLMPAASYQKEQ
                     SSSGRVAAVLFFKTYKALAQTLYLPEIYEANLRYLYLAYDFGHTFLPANKPLSAVPTR
                     GQIEIYEGAGVARFTITELGSDLEIYLLQQEKDALSRNACVIQIYLNLASPATGAAIE
                     ILRKHQYFLGGVLPRWFDSDGLLMQKILHEPNYADIHLYSDRAQKILALIQADREAVM
                     SLQILS"
     gene            21873..23147
                     /locus_tag="P5549_03340"
     CDS             21873..23147
                     /locus_tag="P5549_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRW12140.1"
                     /translation="MISMFFQNLYAVIDTVFISWLGAVPLAAQTFSIPLFYVALSLSK
                     GVQVGTAALISHARGRGETEQVEAIVQAALPLVLLCILPLFMLLIPGVCDAVFGLIGA
                     RGEMLEQIYFYTFWLILGFPVMAYTMICEANFMSHGNTVAPMQAMLLGNGANLILAPT
                     LMFGLDLGIAGASLATLLGQALSAFYIGKKIKQSGLFMPTLKWKAGILAIWKKIGRQG
                     SLVAITLLVSPLGLTLLNGVLSSFGAEALGAWNIMSRLAMLGLLPLNGMAASMIPFMS
                     FNNAQMKVERVNEGIKYFLLLATVFILPIIMIFIAFPHILLLLFQAEGKIMELGSYAV
                     RVAALGYILVPIDLALFSLAQGLQKPVYPLFTLILRILVFRYPLAVYFAATWGVRSVY
                     WCEPVSMGVAAIFSAFLLWRLTNGLKSVVSRY"
     gene            23415..25298
                     /locus_tag="P5549_03345"
     CDS             23415..25298
                     /locus_tag="P5549_03345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRW12141.1"
                     /translation="MCEKGSIIDELKKSHPEKFVSVAEVFKNIRRGNKIFIGTAAGEP
                     RHLVRSLMDYLVNNPKAFFDADIYYVWTVFDESNYFLEKYKRNFRISSFFIGDPNRDA
                     INNGDADFTPIFTSRIPELIDRKMLPVDIALIQVSLPDEHGYVNLGVSVDVAKTAVEN
                     AGLVIAQVNSFMPKVHGDGFIHINDIDFMLPFDEPLPEYDPREESEVVSQIGKYVASL
                     VQDGDTIQIGYGNVPFSILPSLSDKKNLGVHSELLTDGIIQLMKQGVIDNSRKTINRG
                     KTIGTYCLGKKETYDFLNNNPAVELRRIDYVDNLTTIAQHDNMTAINTVLQIDLTGQA
                     STESLGTVYYSGISGFANFMRATPLAKRGKTIVAMKSTAKNGTVSKIVPNLLEGSGVS
                     LHRADVQYVVTEYGIAYLHGKNLRERAMELIAIAHPDFRPWLIEEAKKLNLIFKDQKF
                     VPGPRGKYMEEYEALRETKTGLDILLRPIRISDEQRLRDFFNTLSDKSLYLRFFTPRK
                     YVPHESLQEIITVDNAKGISIVAIDQQEETEKIIGLGQYFLNETNYSAEVSFATSDDY
                     KNNGIASILLTHLTYVAQKNGLIGFTATVLAENRSMVKVLKKMGFTHKSTEEGVSDFE
                     KSF"
     gene            25402..25722
                     /locus_tag="P5549_03350"
     CDS             25402..25722
                     /locus_tag="P5549_03350"
                     /inference="COORDINATES: protein motif:HMM:NF023184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HRW12142.1"
                     /translation="MAFSRSLEIKTLQQMITMYCHAHHSTSRNELCADCSALFEYAGQ
                     RIDKCFYGDDKPVCSQCTVHCYKPEMREKIKAVMQYSGPKMMWTSPILSLRYMYRKLF
                     KSNI"
     gene            25953..26381
                     /locus_tag="P5549_03355"
     CDS             25953..26381
                     /locus_tag="P5549_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HRW12143.1"
                     /translation="MKKMILWLAALMLLGFGMMLMVKIPALGLAEAGFCGKCHAMDYQ
                     VSTYLHSSHAREANCGDCHDPHGLVTGSMYAAYTGSRDVYRVVTNTTPDEIRATELSK
                     KILQENCLRCHGDIMGDVGDTRENGGVYCFHCHQNIVHPK"
     gene            26399..27658
                     /locus_tag="P5549_03360"
     CDS             26399..27658
                     /locus_tag="P5549_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HRW12144.1"
                     /translation="MEAAKIKKWVLAVSVIGMVGILTGVFFFNRNNTVITETARGEIA
                     ATETNSAVWGQFYPAHWDSYQANLSNAAQPSHYDTKLYLQAMYEGTGFAKEYNEPRGH
                     IFSIEDVTSVNPARQKAGASCFTCKSTQVPEMMDKYGAKYYLMSFDEMNGQITQPIGC
                     LDCHDPKTMALRITRPALQEALQRQGRDLDKVTNQEMRSLVCAQCHVTYYFAPETKKV
                     TFPWDKGFTAQQILAYYDEEQFTEWVHPVAGSNLVKARHAEYETFMGSTHQTAGVACA
                     DCHMPYTVVGNKKISSHVWQSPLNNIEQSCTTCHRNGTEWLKNRVETIQTQAKQSQDL
                     AGEAVVQAINELKMARETPGVDQAKLAEAMKLHREAQWYLDYVAVTNGYGFHNPTATL
                     NDINAALDKAHKAVVKAREARGDNNPS"
     gene            27726..28655
                     /locus_tag="P5549_03365"
     CDS             27726..28655
                     /locus_tag="P5549_03365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12145.1"
                     /translation="MDRLKDLGRIKVILGENSGRFPFCNSILIDDSVQAVIDPGAGLQ
                     QLRAINDRQNIEVVFNTHVHFDHIVFNYVFDHSRIMVNEPESIYFRDRRKFIEDFGTS
                     QVWGDAWADHWLEQTARPENLQSKTIAYRHEWYLSMARLDGTYRWGDVFDFGQVKMEV
                     IPAPGHSQGFNVMYFPLQGIVYCSDIDLTAFGPWGEDYDQFVESARRIAQLDADTFVT
                     GHESGIVSKNEFVSRLDQYLELIDRRDDILLEKLSRPRTQEDLIKEGTFYGPRIFRDE
                     FVYCWEWYMVKQHLRRLLKQHRIVEKEGIYSRV"
     gene            28960..30552
                     /locus_tag="P5549_03370"
     CDS             28960..30552
                     /locus_tag="P5549_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12146.1"
                     /translation="MDFIGKQPGKTSLTSNGISTIRGHVQKHPDRIALKTEDFSETYA
                     EMWQRCVRLANALLAKGLNKPDLIATYMPNSYQFVEVIVAAEMIGLPITLGNYRLTPE
                     EIIYQINNCEAKVIFVKQEQYDLISPLLDRLPTIKEIVLISDTPAEGVLNYEELIAGG
                     SQEEPKADVLPEDMHLLFYTSGTTGKPKGAVRTMYCDYNMAISTAIELGLSRDDSMLV
                     VAPMYAAATTGYFLTTLMVGGTLCIAPAFIPEDSLRLIDLHKPSFVFMVPIMFDWMLS
                     LPPETIAKYNLSSVRLAVACGAPMHSTIFQKMADHFTNAKCINMLGASELGFVTSITT
                     EEWFGLKKEGSIGKGIFDMDLKIVDDKGQEVGPDGVGVLYSRSPATFDGYWQNPESTR
                     ESFLNHEWTSVGDMARIDEDGYIYLVDRAKDMIVSGGTNIYPAEVEGVILKLNGIADV
                     GVIGIPDEKWGEQVKAIVVLKPGYSISEDEIIAHCRQHLAGFKVPKSVDYADAIPRNA
                     VGKMLKKELRKPYWEGKKSFIS"
     gene            30696..31298
                     /locus_tag="P5549_03375"
     CDS             30696..31298
                     /locus_tag="P5549_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRW12147.1"
                     /translation="MNQKKPTKRDLQAAETRKKIFDTAFRLITEKGFENVTVEEIAKE
                     SEVAKGLFYHYFKSKDDIVVETYFIIDKSYSQFYAENAPHCDAIEKILLTVVFQARHA
                     TFLGVDFVRQIYKSQLYAGTQYFISKERSFYTILRDAIQAAQIEGTIRDDVSAEKLAN
                     IVLSCGRGMLYDWCLNDGKYNLEERMTNQLTIIIEGMQKR"
     gene            complement(31599..32093)
                     /locus_tag="P5549_03380"
     CDS             complement(31599..32093)
                     /locus_tag="P5549_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRW12148.1"
                     /translation="MFKVSDYVVYSTTGVCRITAIGKDEYAGNNDTEYYVLQPVYDTN
                     MTIKIPVNNPKIAMRPILTQEEVLSLIAAMPTQETIWIEDNRERNELYKTILKRGTCQ
                     EWIKLIKTLHVEKEARLANGKTLTSTDEEIMRAAEKQLHEEFAIALNISPDKVLPYIL
                     EHIS"
     gene            complement(32346..32780)
                     /locus_tag="P5549_03385"
     CDS             complement(32346..32780)
                     /locus_tag="P5549_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12149.1"
                     /translation="MSLEVKRTKIKELLHRKIITPMEHDKIVALLAELADEGEIVAEY
                     KSIYNTCKKNTTKQLIAPNSAVYPDFSVDMIEPLDDNSYLILFYVDSENKKGDMLRTN
                     LKCKVVNGQYEGTSFQIKKVRTRLGGAKITEEWSKFTKPKTK"
     gene            complement(33147..34106)
                     /locus_tag="P5549_03390"
     CDS             complement(33147..34106)
                     /locus_tag="P5549_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW12150.1"
                     /translation="MHTHQDNSLTPQEVAQQLQITKNTVYELVKRGDLPAFRIGRKLR
                     FDQHDVDMYKQTGKFTQPSGPVNQTQPTVKGTCFSYAGAERRQELVICGQDIILDILA
                     RHMERYSGGLKILRNQIGSFDGLMALYHNRADMAAVHLWDGDSGTYNVASVRRLLPGI
                     PVVLVHLAMRMQGFYVKKGNPKNIQDWTDLVRPEIRFINREKGSGTRILLDEKFRSLG
                     YDRLQIQGYTQEEFSHLAVASAVARGEADVGLGNQKAAMQVREIEFIPLQSERYDLVV
                     KQEDCYTDWYKAVLQIINSSEFADELKGLGDYDLHETGRTTKL"
BASE COUNT         9382 a         7205 c         8180 g         9307 t
ORIGIN      
        1 gtaacccaaa tggttgacat gatgaccccg atcgtagccc cggttcttgc cttctacctg
       61 gaaggaggag ccaaaacaat attggttgac actggcgttg tagaacctgg ccccgatggt
      121 acttttcatg gctttccttt aataggcggc ggggcacagg gtatgataaa tgctctggca
      181 gaaattggct taaaaccgga agatattgat attgtaatca taacgcatat gcactttgac
      241 catgccgcta atatttcctt gttttccaac gctcagttta tcctgcagaa aaaagagtgg
      301 gaatatgcca aagctccatt gcccatacag aagggagttt atttgaccga tttattaacc
      361 gaattggagg agtacgattt ggttttggct gatgatggtt acgaggttgc agatggagta
      421 aacgtaattt gtattccggg gcataccaat ggacaacaag ccgtcgtagt caataccagt
      481 gatggaactt atgtactttc aggagatctt atgtattcca tggccaatct atatccgcaa
      541 acaactgaaa ttattgattc actgggaaat aaagttccct gcacgccaca acttggacat
      601 cctttttatc ctcctggaat acatacgaat ctcaatgatt ggtatagcag tgcatggcgg
      661 ctgttgtcga ttgccggaac ccgcaagcac attctgccgg ggcatgatcc cagcctggtg
      721 gggagagttt ttccggaaaa gtagcatcct gcgattagtc aaaacaagaa tcaaggaaag
      781 gggaatatca tgactgcaac aaaattggaa tttccaacaa ccagacttga agatttaccg
      841 tacctgcaag gcagacagct gaattttgac agtattgccg aaatgtttta tacccgggtg
      901 aatgaagagc cggatgcaat tcacgtgctt tattatgacc aggtaatcac gtatgcccag
      961 actaatcaaa gagccaacca ggttgcccac tatttgaagg gaaaaggggt gcaaaagggc
     1021 gatgtggtat ctatcatggt tttaaattca cccgaaaatt attacaccat gtttggggcc
     1081 caaaaacttg gggcagttgc cggtgcagtg aactatatgt tgaaaggtcc ggaaattgcg
     1141 tatcttttgg acgactgtca gcccaaagtt gcttttgtga gcagcgagtt tatgaaggac
     1201 tttgcggccg gttatgcgct ggcccagcat aaaccggtgg tcgtggaagt tgtcaccggg
     1261 gtagaacatg acagccacat taatgagtgt acattggcag acatactcag ccaatatcca
     1321 gtcaccgaaa ccctggtaaa acaaaaccct gatgatccgt ttatgcttct ttattcttca
     1381 ggtactaccg gtaatcccaa aggtatcctt ctgagcaata aaggacagtt gtccatctgc
     1441 cgtgatatga accgcatccg tttgttccag ggcaaagatg taatgatgat cattttgcca
     1501 atgttccata ccaatccgct ctgtgtatgg acctacccct tgacttatgg ggggcatacg
     1561 atctgcatcc gcaagacgtt ttcgccgctg gatttctggc cggcggtcat gcagtatggg
     1621 gttacggtag tgttcggagt acccacgatg tttaactaca tttataatgc tgctgatccc
     1681 aagatcatta atccggctaa actaaagata agatacgctt ttagcggagc cgccccgttg
     1741 ccggtggagt tgattcatgg tttcaaggaa aaattcaata tcgaaatcgt ggaaggctat
     1801 ggcttgacag aggtttccgg ggtatcaacc gtgaatccgc cggcgggcaa aaagatcccg
     1861 ggttcagtag gcctgcccta tccggagcag gaaatcgccc tgatggatga aaataaccaa
     1921 attatgcctc cgggagaacc gggagagata tgtgtacgct cggatgccaa tatgttggct
     1981 tatctcaatc aaccggatgc cactgctgaa acgatcaagg atggctggct gcacaccggt
     2041 gatgttggac gcctggatga atacggttat ttatatatcg ttgatcgcaa gaaagatatg
     2101 atcaaccgtg gcggtgaaaa tatttatccc cgggaaatcg aaattgctat tgaagcaaat
     2161 accaaagtat ctgaagtagc ggtcatcggc gtgcccgatc cggcgctggg cgaaaaggtt
     2221 aaggccttta ttattacgag gcctaatcaa agtctcagtg tcgatgaaat caaagaattt
     2281 cttcaggata aaatagccaa atacaagatt cccgagttta ttgaaattac caatgatatt
     2341 ccccgcaacc caaccggaaa gattctaaaa aaggaattga gggagatgga agcggccaaa
     2401 acaatgaaag agtaattact caaaccaggt gatgttggaa ttatcaaaat agacgaatat
     2461 ccattatggt tggaattata gtattaaggc ttagttgtgt tttatctgat gccgcagtta
     2521 atttgtaaat acaattctcc caaatgttgc tgttataatt gcggcatcag aagatttttt
     2581 aacccatgcc atcagacatt atatgcatac aacagatgtt ggaggaattt aagtgaacta
     2641 cagtaaagat tacagcagca aacttgttac agcagaagaa gcagttaagg tagttaagtc
     2701 cggtgatcgc atattctata gcgaatttgc catgttcccc aaaatgttgg acgccgctct
     2761 ggccaagcgc atacctgaat tgcatgattt ggaactttac agtgttttct atacccaggt
     2821 gcccaaagca atagaggctg atccgcaaca tgagcatatt atcatccagg attggcattt
     2881 cgggccggtc tcacggcacc ttgctgaaga agacaagtgt ttctatgtac ctatcgcgta
     2941 tcaccaggga ccgcggatca ttcataagta tattgagaat gatgtagcta tggtggcagt
     3001 tagtcccatg gatgaaaaag gattctttaa tctgggccca accaattcag ttaccccggc
     3061 ttatctggac aggtccaaaa ttattatcct ggaagtcaat aattctgttc cccattgcct
     3121 gggaggcaac agcgaaaata ttcatattaa tgacgtcgat tatgtagtgg aaggcaacaa
     3181 ttattcgctg atccagctgc ccgctgccaa acccaaaccg tttgactgtc agatagcggg
     3241 ttacattatg aatgaaatcg aggatggtgc ctgtctgcag atgggcatcg ggagcatgcc
     3301 caacgtggtc ggagccaata ttgccgacag cgacctgaag gatctcggta ttcataccga
     3361 aatgctggtc gattcatgtg tggatttgta tgaatccggt agggtgaccg gcaaccgcaa
     3421 aagcatcgac aaaggcaaga tggtatacag cttcgcgctg ggtaccgaca agctttatga
     3481 ctttatgaat aataatccag tgtgtgcatc ctatccggtc aattatacca atgacccgcg
     3541 catgatcgcg ctgaatgaca aggtgattgc catcaacagc gcgttggagg ttgatctttt
     3601 gtcacaggtc agttccgagt catccgggta ccgtcatata tcaggaaccg gaggccagtt
     3661 ggatttcatt atgggcgcgt ttcaatccca tggcggaaaa ggatttatat gtttgagttc
     3721 cacgtatgag gacaagcacg gtcagcttca ctcccgtatc gtacccagcc tgagagtagg
     3781 ttcagtcgtt accgtaccgc gttccatgat tcagtacgtt gtaactgaat acgggatcgt
     3841 tcaactaaag ggaaaatcca cctggcaacg ggcggaagcg ttgatcagca ttgcccatcc
     3901 agattttcag gatgaactgg tcgaacaagc acagaagatg aaaatctggg ccagaagcaa
     3961 taaaaatgag gaaaaacctt aaaacattgt cccggctgac agttacagct aaacagagat
     4021 ttttcttgat tatgatttat atgttttggc atctgttaaa tagcagatgc ttttttatat
     4081 tcatattata aaattaattt aaccacagaa taattgttaa aattaaaaca ataattaata
     4141 atattaattt ttaggttgac aatgttaata acatggttta taaaataaac atacgcaatt
     4201 gattttgaag gaggggaata aaattcagta taaagcaccc agccaatgtc ctgtatgcgg
     4261 acaggaattg ataatcagca aactcagttg taaacaatgt cagacatcaa tcgaaggctt
     4321 gttcagtccc tgcaagtttt gtcgtttacc aaaagatcag cttgatttta ttgaaacttt
     4381 tattaaatgc cggggcaata taaaagacgt cgagcgggag ttgaatatat catatcctac
     4441 cgttcgcaaa aatttggata cggtaatcca gtccttggga tatcaactgg aacgttcaga
     4501 ggaaagggaa gaagaaatcg ttttgcggcg taaggagatt ttaagtgcat tggaacgcgg
     4561 ggagataaat tccaaagaag cagccaaaaa attaaaggaa tataaacgct aagtcgaccg
     4621 gctgatgcca ggatgtaaat ttgtaaagtg gaaaggagtt ttaactatga atcaggaaac
     4681 catgaaaatt ttggaactgt tggaacaagg aaaaattacc cgccaggaag ccatggagct
     4741 aataaattca cttgaaactc gcagcgattc cgaagggggt gctccggttt ctggtgaaaa
     4801 taccaaacgc tttttacggg ttcgggtgaa tggagataaa accaaagtaa atgtaaatat
     4861 ccccgtgaac ctgataaaag tagccaccca aattgccgat gccagtatga aatggatacc
     4921 acaggaagca cacgagcaga taaaaaggca gggtattaat ttgagccaga ttgactttga
     4981 tgaaattatc aagctggttg atcagggttt aagtgatggc cggctggtgg atgtcgaaac
     5041 tgaggatgaa aaagaaggca gaatcaaggt agaagtatat gttgaataaa aggagcaact
     5101 tactgctggt gcggatacaa gccgggcaaa aacccggctt gtatattccc ttgccattgg
     5161 tggttttaga tgtaactcta gcatctttgc atgaatgcct ggagttttgg caggttctgt
     5221 ttctaaatct ggttgtcaga aaaattggcc ttgacgaaat aaaaaacagg ccgaagccgg
     5281 cagagatact ggccctgctg ataaagatga tcgacgaaat tcgcagctat ggaagctttg
     5341 aacttgtctg gatggaagat gaagaaaata aaatttccct tcgtttgtat taaaatactc
     5401 gccaaattat taaaaaggaa ttatttatta tgaaacgatt tatctggaaa agcattgtca
     5461 acatatgtgt gatctattcg ctgtcactct ttcttccgca ggttttttta cagggagcgt
     5521 ttccggctat cctggccggg gcgctactca gtctgctttc cgtcaccctc cggccgctgc
     5581 tgatgcttct ctgtctgccg cttaatctct taacgctggg cttgtttgtt ctcgttatca
     5641 atacctggat gctgcagttg acggatctta tggtaaaggg atttgtggta tcagggttct
     5701 ggtatgcctt gctggctgcc ttgttcatca tgttggccaa taaattggtt aatgttttgc
     5761 gatataactg attaaaaaaa actagacatt tatttacttt ggatttgctt tattcttcag
     5821 acagattata aacggatact tcaaatcttt gctttgttct caagtcctga acctggatac
     5881 gattagcagc caccttctca atccatacag gagagccttg ataaaatact tcaataactc
     5941 ccagtgagtc aaatatttcg ttagctcttt tgatatccat aaatcttact cctaatgcaa
     6001 tttttcatag catgtcttat aataggagta attattatgg cagaaaacac caaggggatt
     6061 taagacatag gcctgaaacc cccttggata cctgtcattc agataaatgt aataaacccg
     6121 tacacaagta tttttctcct cggtctccgg gaataccacc acaaagcctt cctctatgtc
     6181 tgtgatgcat taaacaaaaa taattagcct cttctggcgg ccagcgtttc tgccagcgcc
     6241 tgcagtttca tattatttct ttctccgtca tagaaagtct catccactaa atcgccttcg
     6301 atgatggtca ccggcacatc caggcttttt tgcacatgct cggcaatata gcgcatggtg
     6361 atggagaagg gtttgcaact ctgggcatag gcaaagattc cgccttccaa attataattg
     6421 cgaaagatat tttcagttat tttggttttg aaactgacgt tatgatttaa aatcatggta
     6481 gccacacctt ccgctacgct atccagggga cgtgacgaat cgagctgttc aaaggaaaac
     6541 atatcaaaat aaccactggt gaccaggcac agacccaggc tttccagttt ctcagacagc
     6601 tcccggatgg caaaccagta ggggatacca tcccaataaa tacgatgttt catgttggac
     6661 atggggatga ttttgtcggc gatgcgctga ttcaattctt cggtcatcaa acgataatgc
     6721 tcagcggttt caggcagata ccgcaaaacc agattgggga aattgtgtcc catgatatca
     6781 aaaaaagtag ctggcaccgg atcatgcttg ttggcttcca gcatggccat ataatacttg
     6841 gaactgcgct ggccattggc tacgatctcc tgcaggcggt cataatcaaa ctttttcccg
     6901 gtgaactctt ccaggtaggt gattaggtcc aaacattggc gtttgacatg gtctacgaga
     6961 tatttttctt cgtaggcagg aataaccaag gggcagtcga tattgaacat cggaatgttc
     7021 cataattggc tcagatgttc ccaccatttt gttatcagaa tgcagctatt gtttgcaacc
     7081 aacagaaaat ccggacgggc cagctctgaa aattgagaca ttccttccag ctgggatatg
     7141 gaagccccca agctgttgcg gcaataggtg cacagatgat gctcatagcc tctttgttct
     7201 gtaatttcaa catgggaatg agcgatgtgt ctggcgccac aggtagcggc gtaggcttcg
     7261 ggaaactgta cacaaaagtc catcgcccaa agaatttcag cgggcgttcc ggctgatacc
     7321 caggcgacgg gcttggattt gtttttttcg atttcaagtg cgcccccgta atgggccatg
     7381 atcattcctg ccagacgttt ctggccttcc aatctgcgaa ccactttata ttctgattct
     7441 gccatgcatc ttgcctcctc actttttatc accgcaaggc gttgattttt catagaataa
     7501 tattgaattt tttaaccgca ggggagaggc gttagctttc ccattgccgg gccaaaagag
     7561 ctgctcccag agcggttaca atctgcggat cttccggaac tgaaatttta cgtcccagca
     7621 ttcgttccaa taggtttacg atggcaacgt tcttggcgac accacctgtt agaacaactt
     7681 cggcctgggg atccggatta accgagctga tgagtccgaa tacccgactg acgatcgatt
     7741 cgtgcagtcc ggcggcgata tcttccttgt tggtttggcg ggcaatcaag gaaacgatct
     7801 cggtttgggc aaagacggta caagtgctgc tgatggatac tttgttttgt gactgctgtg
     7861 aaatatcgcc catttcactt accggcaccc ccagcgtttg ggcagctact tccaggaatt
     7921 tacctgtgcc ggcagcacat ttgtcattca tggcaaactg tacgacccgg ccatttttat
     7981 caaatttaat gactttgctg tcctgtccgc caatatcgac caccagcgag gcgttgggaa
     8041 acaaataatg aaccccatac gcattacagg taatctcagt gattgttgtc gtagcaaagg
     8101 gaatgctgat ccgaccgtaa ccggtggcta cgatgcggtc gagatcctct ggctgcaaac
     8161 cggcattttc gcaagcctca gcaagaacct ggcgactgac tgagcttatt tcaattccgg
     8221 taggctgcag cgcataaaca aattgatcgt cggctttcag gactacagct ttcgtcgtgg
     8281 tggaaccaat atcaataccg gcggtgatca tatataaaaa cctccactaa acaaattgta
     8341 acgtttctcc gaaggcctga actctggtgc ggatcgcttc cagggaacct gaactgattg
     8401 atatataggg gataccttct tcttcaaaac ggtttttcat taaaacgaat tccatatcat
     8461 acggatcaca gtacatttct tttaatacca caaccccctt ggcctgacca tgcttcatca
     8521 gatcgatcac gtattcagca tagggagagt agatcttgtc aggctgaatc caggcgtcgg
     8581 catagtgacc ggtagtacag ggaatggcgt tggcattgaa atcaatgatc gcgtcaagcg
     8641 gatcgtcgtt taaagcaata tctttactga aatatctgcc gccggtaaaa aagtcatcat
     8701 cgacgatgag catcccgttg tcttcgatca tatcaaggat ttccctttcc ggcatggaac
     8761 agggatgtcc gacagcgatg atccgctgtt ttttgccggc agacagttcc tgttgggtga
     8821 gttgggcgag cagctttgcc aacagttcgt tatgttcttc tttggggagc aacatgctgg
     8881 atgcaactac gcaggccata tccgaagcac ggataatgcc ggggtgttcc ttgcgtaatt
     8941 cgttcagctg tcgcatcaga ttccgtgatt tgttatacag acgaatactt gccttgagtt
     9001 tttctgtcgt aattttgttg ccggtgaatt cttccagacg ggcgatcagt ctcttcagtt
     9061 ctctatggaa aaattcctgg gtggaactgt cccacttata gggacgccac atctgatgga
     9121 agaaatcgac cgggtgatcc aactgccata cttccagata aaatctgacc tggtcacaga
     9181 tatagatcgc agcaattccg tccagaaaat cgtatttgcc cttcaacagg ctgtcaaagg
     9241 aactgcgtac ctgatcacag gcattggtca tcagatgccc gtctcccaat gtaatcggat
     9301 catcgctgcc gaacaaggtg accggcagtg cccccgccgc atgaatgatc tcttccggga
     9361 aatacatggg aagacacccg ataattttgg tttttccggc tgcttttatc tgcgcagcac
     9421 gctcgtaagg cttttcacaa tagtccctga atgtagcaag aatttcctcc aaattaatcc
     9481 ctccaacttt acaggctcgt tgatacggtc ttctttttac aagtttaaac gattctgcga
     9541 tatcagtata gttattcttc ttttcttttc gtcaagtgca ggtgagaaga tcgtaaagat
     9601 tccgccacga ctgacaaaag acagcggttt ttcatgaaag acgcataaac caagggacac
     9661 aatgaaacat ttttatcaat gttcccttgt gtcctctgtg tcatgcttgt cttggtgctc
     9721 tttacaatga ttaaaagcag tttcttgttg ccggtgcagt tagctttttt tcattttggc
     9781 cagttcttca tcccgcaaaa tccttttcat ggctttacca gtggcggaac ggggcagact
     9841 ggccctaact tcgatgcttc tcggcacctt gtatgcggcc agatgatcgc ggcagtaagc
     9901 gatcagctcg ttttccttga cagtggcact ttcgatgagc tgaacaaatg atttaaccgt
     9961 ttcaccgcga tattcgtcag gaatcccgat ggtcatagca tcagcaactt gcgggtgctg
    10021 cagcagcagc tgatcgactt cctggggata gatattataa ccgccggcaa tgatcagatc
    10081 tttggcccga tcgacgatga agatatatcc gtcttcatct tgataagcga tgtcaccggt
    10141 atgcagccat ccgtctttta attgctgggc ggtttcttca gggtcattcc aatatccttt
    10201 catgaccatg ggggatttca taatcatttc gcccggtttt ccgcggggaa cttcttcgcc
    10261 ggtgtttaca tcaacgaatt taacttccgc gtcaggatat gggatgccaa tgcttaaagg
    10321 ttttttcact cccaggaacg gggtggttat ccctacggaa gttgtttcgc tcatgcccca
    10381 gccttcagat ataataaagc ccaattcacg agcttttttt agcagatcca aagaacacgg
    10441 ggcggctccg gcattaatga cagcaatttt tttcgccagg tttatttctt tgacccgggg
    10501 atgattgata atggcgccca gcatggtggc aacagccggg aaaaaccaga catgaggtat
    10561 gcgttccatg gtattcataa attcatccaa atcaaaacga ggcaaaataa tcagcgttga
    10621 tacggttatg gcggacatgg cgatgccgca tatttctccg aaaacatgga aacagggcag
    10681 cacgcccata atgttgcgcc gctcaatggg aatgaattca ctaacggaac gcccccattc
    10741 attgccggta tataatgcag ctaccaggtt gcgatgggtc agcgtagctg ctttcggtgt
    10801 gccggtagtt cctccggtaa attgcaacac ggctgcatct tcttccacat taaacgcgag
    10861 ggaagggggc gccgtattgg cactcccggc caggaaactg gagaagtggt gccagccttc
    10921 cggcaagcca agctcttccg gagtgctcac tttcatttcg ttgatataat cagtcaattt
    10981 ggtaaccatt acaaacgaaa tattaaccat ctgacataat tgctgaacac cggcgattaa
    11041 tccgtcaaag gtgatcaatg cagtcatgcc ggttttttcg atcatgcttt ttaattcttc
    11101 cggtttgtac atggggttca ggttgacgat tacagcgcct gcttgcagaa ttgcccagaa
    11161 ggagacgatc atctgcgggc agttgggaag caggatccct acccgatcgc ctttttttat
    11221 accagcttcg atcaagccat tggccatacg agtagaagag ataaataaat cccagtaggt
    11281 gatttcggtt ccgtaaaagt cagtggcagc tttgtcagga tttgtagttg caatcatatg
    11341 caggatttcg tgggcaggaa atttgggata atttaatgtc ggtcgggtga tgttgtcgta
    11401 acttttcaac cagaatcgtt gttccatcat tgcaggtgtt aatccggtca tttatattcc
    11461 tccctataaa acgtcatatt tgcttgatag aaatgtattc acccccaaaa tcatattgcg
    11521 tggctgtggc gttggtatcc cacctttctt cggtgaattg tgccgttcaa ttcacacgac
    11581 tattttcatc acaggaatag ctgttttccc gcgaaatttg tttcattctt cgtgttgcat
    11641 tggacaaaag aaacattaga aaaggacata acaaaaggaa caagatggtg ttcagtttgt
    11701 aaacctgctt gccaatgatg cgattttcgg attcatgctt gaatattacg agactaaccg
    11761 tatcaggcaa tgaaaatgcc tgacaatcct ttgggaaagt cttggtttga tgttttgctg
    11821 cgaagattta tagaagatat tttgctggat gaattgctgc aataaaagtt ttttattaat
    11881 gttaggcgga gtttgtatta tttattcgaa taaacatatt aaaattgtta atatctgtat
    11941 aatcagctta caaatagtat tgtattaaat attacagctt attacaacat tatggcgaga
    12001 gttcatggtg aaagaataag agcgggttaa caatgacgag ctgcaataag ccagggaggg
    12061 agaaggggac tgtttgcggg caaggaaact agcgcatcca gagttatgat tccggggatg
    12121 acggcagcgt gatttgcact accccaccgc ttgttggagc attacaaagc ttaatggcac
    12181 cggcgtgttc ttcgacaatc tttttgacta tggtcaaacc caatcccgaa ccttttttat
    12241 ccccggtata aaaatagtcg aaagcgtttt cgagatcagt tttggtgaaa ccggcaccgt
    12301 catcggtgat ttttatcgta acctgggtgt tgtgattccc aagcgcagca gtgactgtga
    12361 tgttgtggtt ggcatgacgg atggcatttg acaaaacatt gacaaagaga ctgtgcaatt
    12421 gatcccagtc accaattaca ccgatcgcag gagagatttc gcacttgatt tgaatgcctc
    12481 tttccagtgc gtaaccgcct acagtttcca gggcttgatc cataatgtag tcaagatcat
    12541 ggggcaatag atatgtttcg tgaggctgat ccaggatcgt aatgtcaatc atgttctcca
    12601 cgatatcccg caatcgctga ctttctttat gaatgatatc aatcgcatgg tcaagagatg
    12661 gtccgctgaa gatgccgtcc cggattcctt cggcgtaacc gttgatgctc atcaacgggg
    12721 ttttcagctc atgggaggca ttctgaaaaa aacgactccg gctttcatca tatcgctgca
    12781 gtctttccgc catgtcattc aacgcaatcg ccaacttgga cagttcatca tcagatttta
    12841 attccaggcg cacgtcaaac tgacgattgc ctactgcttt tgcatagttt tccaaggaac
    12901 gaagcggttt caccaaatac cggatcaaga aaaaagcaat caacaaggcc atcgcaagtg
    12961 caactgccag actgcggaaa attgttgcca gggtttcccg atacatggac tgcagtttac
    13021 tgagagcgac aaaagtcatt actgttccgc cgttgcttgc cggcacttca acggtcagat
    13081 aaaaatcatt tttaaaaaca ttgacccggg agcggtcaaa gggctcttcc tgacgtaaag
    13141 acagttggaa aatgcgttgg ccgggtgtaa acttctccgg ccagctgctg taaatgattt
    13201 ggtcttgcgg gctgatccat aaaaaatcgc tctccaccga acgccccagc aacaggatgg
    13261 tcaagggcgt tttattatcc tccgaaggaa cggttcccga gccaatggca tgggagattt
    13321 cccaagcttg cttttccagc ccgatttccg cacgtttttc caaaactcca accagaatcg
    13381 gataaaacgc caaagccgtc accaggaagg taaacaacac cacgctcaaa aaacctatta
    13441 atattttagt agatatgctt ttccactttg ccatctaatc ggtaaccgta cccccatatc
    13501 gttttaatat tggttttaga attactttcg ttgagcttgc ggcgtaaacg tttgataaga
    13561 tcatccacgg cccgttcgct tccctcataa tcataacccc atatgcgatc cagcagttcc
    13621 tgacggttaa aagttcgctg aggatgttgg attaacaaag ccaacagatc atattccttg
    13681 ctggacaagc tgatttcctc gccatcgatc aataccagcc ggtcctgggt gactaatttg
    13741 agattgcccg ccaacatata tggttgggga tcaggcggga ccatggtccg gcgaaaaacc
    13801 gagcgaaccc gggcaaccag ttcccgggga ctgaatggtt tgggcagata atcatcnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcg aacccgggca accagttccc
    13981 ggggactgaa tggtttgggc agataatcat ctccgccgat ctccagaccg gcgatccgat
    14041 ccagagcctt gcggcgggcc gagataaaaa tgatcgggat ccgactgtgc tgtcgaaaat
    14101 cgcggcagaa ttgcagacca tcttgtccgg gcagcataat atccagaatt aacatatccg
    14161 ggtagcttgt ctccagacgg cgggacaggt cctccacatt tccgaaagcc tccaccacgt
    14221 atccctcatt ctgaagatat tgcatgatca attggcggat attttcttcg tcatctacta
    14281 caaaaatcgt attcactgca ctcactcctc agatgttcag gcaactgtta aagatatata
    14341 cagcgtaata aaaagatgtg gcaaaaacat gtgagcttgc cgtgcaatga aacccttttc
    14401 ccgggaatcg ccaaataatg taagaggttt tgccccaatt ttgccacaaa tatagcttac
    14461 aatttaaaag atcggattgg catggaaaaa atctatgagt ccgggaacta ttagatttta
    14521 ttgtagcatg aattgctccg gttataaaaa gggaggtacg ctaacggcta tataaggttg
    14581 ggaagcactg atatatactg accctgccca acaatctatt tgtaaatcgc gctgagtctt
    14641 tctttgcaga aagaacgggg gaaccaattt atggggtgaa tccgcaaggt agggaaatcc
    14701 cttttccgaa cccgacagct aacttcgtaa gcgctttaag ggaatccgag gttaatatat
    14761 ttacaaaacc aaaggaagac ctttggtttt ttgtttctga aacagttggt aagtccaata
    14821 ggtttgagaa gtgattgaaa aactgaatat gcaaggagga aaaaatggaa cagatttcag
    14881 aaaaaatcat cgtaaaagac ttgattaaaa tctttgaaag tcatacacaa aaggaattga
    14941 acctgttgaa aacaggaaaa agcaaagaag aaatgcgccg ggaaacgggt ctgacggtgg
    15001 gcgtcaataa tatcagtttt actgtcaagc agggtgaaat atttgtggtg atgggtcttt
    15061 ccggaagcgg caagtcaacc attctacgct gtcttaaccg gctgatcgag ccaagtgcag
    15121 gcgagattat cattgaggga acagatatta cccagttgaa gagcaaagaa ctgcagagtt
    15181 tccggcaaaa aaagactgcc atggtatttc agcaatttgc gttgctgcct caccgcaaag
    15241 ttctgcagaa tacagtatat gggctggagg ttcagggtgt tgccaagaag gaacgagaag
    15301 aaaaagccat gcaggccctt gaactggttg gcttaaaagg ctgggaggac aaatatcctg
    15361 cgcaattgag cggaggcatg aagcaaaggg taggtctggc ccgggccctg accaatgacg
    15421 ctgatattct gctgatggat gaggctttca gtgcgcttga ccccttaatc cgggaagaga
    15481 tgcaggatga gcttttaaat cttcagcaaa agatgaacaa gacgatcgta ttcattaccc
    15541 acgatctcaa tgaagcactc aaactgggcg atcgcattgc ctttttacgg gatggggcca
    15601 tggtccaggt gggcaccccg gaggagataa ccaccagtcc tgcggatgat tatgtcgcca
    15661 gatttgtcag gggggttgac atgagcaaga ttctgaccgc cggggatgtg atgaaaaagc
    15721 cgcagccatt gataaccctc cgggatggtc cgcatgttgc tttgcggatc atgagggaac
    15781 aagggatatc aagtgtattt gtggttgaca gaaacagaca tttgcaaggc atcataaccg
    15841 tggatatggc ggtggaggct gcccgaagcg ggattatcag tttaattgat gtccaactgg
    15901 cggaaggacc ggtcgttcaa agagatactc ccgttttgga tacgttggga attatcgccg
    15961 aaagtaaact gccaatggcg gtggttgatg atgagaatcg attgatcgga atcattgtcc
    16021 gcggtgccgt gctggcagcc ttggcctcat ataaaggagg tgaggaaaat gaataaattt
    16081 ccgctggacg cgctgattga tcaggcagtt aaatggctcc aaataaacta cggcgaaagc
    16141 gttgatgcat tttccgctgc ttttagtgaa agcatcaatt ttatgggaga ttctttggca
    16201 atggttccct ggtggctgat aatcctcgtc tttgcggtta ttgcctggcg ttgcaacggc
    16261 tggaaacttt cgctggggac ggtcataggc ttgtatatca tttataacct taggctatgg
    16321 gcaccttttt tagatacatt gattctggtc attatttcgg cagcactttc tattattatc
    16381 gggattccgc tgggaattat ggcggccaaa agcaataaat ttcataaagg gatttccccg
    16441 gtactggacg tcatgcaaac catgccgtcg tttgtctatc tcattccggc gttgctgttt
    16501 tttgggattg gaaaagtacc ggcggtattt tccacggtta tatttgccat gcccccggcc
    16561 attcgcttta cagacctagg catccgccag gttccgaccg atctggtgga agtaggggag
    16621 gcctttggct ccagcccggg acaacttctt tggaagattc agatgccggt ggcattgccg
    16681 acgattatgg cgggcatcaa ccaaaccgtg atgctttcct tgtccatggt ggtaatatca
    16741 gccatgattg gcgccggcgg acttggcgcc ggggtactgg aagccatcag ccagctcaag
    16801 atcgggatgg gatttgaata tggtatcgca gtggttatta tcgcgattat tttggacagg
    16861 atttctcaag ggattggtcg ttctttagga acggttgata ataaaaacaa caaggaggaa
    16921 taggaatgct caaaggacaa aaaaggaagt acaaaattct gattgtagcg ctgattgtag
    16981 cgttgacggc aggtttggtg gtggggtgtt caaactcaca acagccatct gcagacaaag
    17041 acaaaggcac gatcaatatt ggctatgtca actgggcaga gtgtgtagct accagcaacc
    17101 tctggaaagt gattctggaa gagcagggtt atacggtgaa acttacccag ctcgatgtgg
    17161 cgccggtatt tgttgggttg agcaaaggcg atattgactt cttcatggat gcctggctgc
    17221 ccataaccca tcaaacatac tgggaggagt ataaagacaa tttggaggat tacgggattt
    17281 ggtatgaagc accggccaag attggccttg ccgtacccca gtatgtgacg attgattcca
    17341 ttgcccagct taaagatgaa gcaggaaaat tcaatggtca aattattggc attgatccgg
    17401 gtgccgggat tatgaaggca tcagaacgag ccaatgaaga ataccaactc aattttgagg
    17461 tggtccaggg cagtgaagcg gccatgatga caacgctgga aaaagcgtat ggcaagcagg
    17521 gatggattgc catcacgggt tggagtccgc actggatgtt tgccaaatat gatctgaagt
    17581 atttggaaga tccgaagaaa tctttcggtg aagaagaaga aattcatatt ctggccaaca
    17641 aagactttag cagcaaacag cctgatatag ctgccatgct gaaaaaattc agaatggatg
    17701 acgctcagat cggcagcctg gaggcattga tcaatgaagg catgcaaccg attgatgccg
    17761 ccaaacagtg gaaaaatgac aaccaagata tcgtagacag ctggacaaaa tcgtaaagtg
    17821 ctgccataaa acagatgata atcagattaa atgatgatgg agtatgttct ttatgactgc
    17881 catcattgaa aaaggagttt cagatcacaa cgctgaaact ccttttttga ataaaaaata
    17941 gagtgcttcg ctgccaaatg ggcgtttgta tatgagcgga ttgcagtaag cactgttaaa
    18001 aactacgcaa tgaataagtt gaaaaacaaa aagggatttt ataaatacaa gaagaacatc
    18061 taaatttgat gaacaaaaga aatcagggtg ggggagttat tatttttacg ctggtgggct
    18121 gatttctgtc aaaagtaatt acctgagagc gcaaacagat gcgcttatat ttttatctta
    18181 ttggcgatga gaatatcttt aaagtctgaa gcagaaggag agaagtatca tgccatttcc
    18241 caaaaacgaa atcgtgatgg agttaagttt gcatccggag aatttgccag ttgttatgaa
    18301 catggcagag caagccgctt tggtatttgg catgcaaagc aaggatacca tggcattaag
    18361 actggctgcg gaagaggttt ttagctattt gtgcacgcat acagatatgc aggatacgat
    18421 ccgtttagaa tgtaaaaagg ggatttacta tattcaaatg gattttatct tccagcacag
    18481 caatctcgac cttagagcat ttaatatcag cacggatata aatctggagg atgacagtca
    18541 acttgaggaa atgggtctgt tgatcgcagc tcgcagcatt gatcggctat ttttgaatga
    18601 cgaacagaat cgaatggttt tgtctcttta taaggaaaag agctatcctc ccgctgaaat
    18661 atctacccct atgagccgga acacaacaga attttttatt aaaccagctg gtatcgaaga
    18721 tttaaaatct ttctgccagc tagctgcgag ttttgacaaa gatacccagg agttacctgt
    18781 ttttctgcgt tatccgggca agtttgcaga tatgatttat tccggggaat atcaagcagc
    18841 actggctttt gaccaacagc aagcgatttt gggagggatt gtctggaaat cccaggcaca
    18901 aagaaccata gaattttacg ggccctatct ttttacccgg tttgacggca tggcggaagc
    18961 cctgatcgaa agctgtctcg agcaaatcgg tcgctcagat gcagtcggac ttgtcaatcg
    19021 atgtgggacg gtggacttga gcgaaaagta ttttgaaaaa ctgggaacca tccagtactt
    19081 gcataatgat gacaaagtac tggatattac agcatattac cggcaattat gtgaagatcc
    19141 cgggcaaaga atatggaccc atcctgattt attgccttac ttgcaaagtc aatatcagcg
    19201 gttgtttttg ccccgagaga ttagaccagt tagcaacatg ggcgaataca aacacgcgca
    19261 ttcagttttt tcatcggaaa ttggccatga ccaggtgatt ttgcggccgc tccttggggg
    19321 agaggatact gctgccaacc tgaaaggaca tttacagctg tttgcgaggg agaagattcc
    19381 caacatcttt tttgaaatcg atttggggct agcttggcac gctgaactgg tcccggcttt
    19441 gttatgtcag ggatttaaac ccggtttaat tttaccgtat gcgggcaagg ctgacctgtt
    19501 attgatgcag tatcgggcag gtgatgtgac atgctaaacg tagctttgga tcatttggtt
    19561 ccatcttata taaataattt tgatgcttat attcccagca aaccagaccc ggaattaaaa
    19621 aaaatgttcg gctgcgacac gttgtatcgg ctgaacaata atgaaaaccc cctgggtact
    19681 ccaccggccg cgcaggaact ttttcaaaaa tttaacccgg ctcgggcagc gatctatccc
    19741 agtggtgatt catattatct gcgggagaaa ctggcggaca aattcagcct tcacccggat
    19801 caaattttgg tcggtaatgg tgccaatgaa gtgattgcct ttgtcattaa tgccttttgt
    19861 gaatcgggag acaacattat cacggctgac aaaacctttg ccacgtatga atggatagcg
    19921 acattttcag gctatgaggc ccggctggtg cctttaaagg agtatgcctt cgatgatcag
    19981 gcaatgctgg aacaggtgga cgcacgcagt aaaataattt tcatctgcaa ccccaacaat
    20041 cctactggaa cttactggaa ccaggaaaag ctggttcgat ttctggacca ggtcgatggt
    20101 cagcaaattg ttgtagtcga tgaggcttac tgtgagtttg tggaaaagga agattttccg
    20161 gataccctgt cactgcttga gaagtatccg aacctgataa tttttcgcac cttctccaaa
    20221 atgtacggat tggcaggctt gcgtatcggc tatttgatag gcaatttgga ggtcgtcaac
    20281 atcatccgta aaacttgtgt ggtttattca gtcaatacta tcgcccagga agctgcactt
    20341 ttagccttgg atgaccagga gcatgtgatt cgaacccgga aactggtggc ggaggagaaa
    20401 gcctatttac gacaggaatt tgatagattg ggcctgtcct atgtcatcaa tgaaggcaat
    20461 tatgtcatgc tcaaactgcc gattagtgac accctggctt atcgcaagct aatgcgggaa
    20521 ggtgttatgg tacgtaccat gacgggattt cgatttccta attatattcg cgttaccatt
    20581 gcccagcacg aagcgatgga agctttcata aggggattgg agaagattct ttaatgtaaa
    20641 ctgcataagt ttaaatgagc agtatttatt cttcagaggg ggttatagca ttgaagaatt
    20701 ctacaaaaaa tgacctggag ttggatattc ggctaatgtt gcccgaagat gctcctggcg
    20761 tggtggaatt atatcgcagt gtatatggtg acgaataccc gatcaagtcg gtttatgatg
    20821 tggaaggcat catacggcag caaaacaacg gtgatatgat cagaactatt ggggtttcta
    20881 ataagggaca ggtgttgggc caaactgcta tttatcgttc ttgtgtcagc aatccggctt
    20941 tatacgaaga aggacaggcc attgtattgc gggaatacag aaaccaaggt attctcgaac
    21001 gttgtcatga ttacgctcat aatcaggtct atccccaaat catggagcag ctatgggggg
    21061 aggcggtctg taaccatgtt ttcacgcaaa aatcaagtat tcgtgccggg gggtatgaaa
    21121 cgggcattga gctggattta atgcccgccg cttcttatca aaaagaacag agcagcagtg
    21181 ggcgcgtggc tgcggtgctt ttctttaaga catataaggc tttggctcaa accctttacc
    21241 tgccggagat atatgaagcc aacttacggt atctctattt agcctatgat tttggccaca
    21301 cttttcttcc tgccaataaa ccgctttctg ctgtgcctac ccgtggtcaa attgaaatat
    21361 acgaaggtgc cggggtggct cgttttacta ttactgaact gggaagtgat ctcgaaatct
    21421 atctgctgca gcaggaaaaa gatgctttat cccggaacgc ctgtgttata caaatttatt
    21481 taaatttggc ttcccctgct acaggagcag ccattgagat actgcgtaaa catcagtatt
    21541 ttttaggagg ggttttgccc cgttggttcg acagtgatgg tctgttaatg cagaagattc
    21601 ttcatgagcc taattatgcc gacatccatc tgtattcaga ccgggctcag aaaatcctgg
    21661 ccttgattca agctgatcgg gaggcagtta tgtctctaca aatcctgtca taattacttg
    21721 cgaggatgag tgtaaatcgg taaacagttc agatgtaatt tatttaggca cattgcggaa
    21781 agggatttat tgtgagattc attgcatcca agtctgtaga tatgggcaat ggccctattc
    21841 tgccaataat tttaacactt gcaattccgg ctatgatctc catgtttttt cagaatctat
    21901 atgccgtcat agataccgtt tttatctctt ggctgggcgc ggtgccgttg gccgcacaga
    21961 ctttttccat accgctgttt tatgttgctt tatccctgag caaaggggta caagtgggca
    22021 ctgctgcctt gatcagtcat gcccggggac ggggagaaac tgaacaggta gaggccatcg
    22081 tacaggctgc gttgccgctg gtactattgt gtatcttgcc tttattcatg ctattgatac
    22141 ccggggtatg tgatgctgtt tttggcttga tcggtgcccg tggggaaatg ttggaacaga
    22201 tttacttcta tacattttgg ttgatccttg ggtttcctgt catggcctac accatgatat
    22261 gcgaggcaaa ttttatgtcg catggaaata ccgtggcgcc tatgcaggcc atgctgttgg
    22321 gaaacggcgc taatttaatc ctggctccca ccctgatgtt tggtttggat ttggggattg
    22381 ccggagcttc tttggctacg ttactgggac aggcactatc agctttttac atcgggaaga
    22441 agattaaaca atccggtctg tttatgccaa ccttaaaatg gaaagctggg atcctggcaa
    22501 tttggaaaaa gattgggcgc cagggttcgt tggtagcaat tacccttttg gttagtccgc
    22561 tgggattgac cttgttgaac ggggtgctct catccttcgg tgctgaagcg ttgggtgcgt
    22621 ggaatattat gtcccgtctg gccatgctgg ggttattgcc tttgaacggt atggcggcct
    22681 ctatgattcc attcatgtct tttaataacg cccagatgaa ggttgagcgg gtcaacgaag
    22741 gtataaagta ttttttatta ttggcgactg tttttatttt acctatcata atgatattca
    22801 ttgcctttcc gcacatcctg ttgttgctat ttcaggcgga agggaaaatt atggaattgg
    22861 gaagctatgc ggttcgcgtt gccgcgctgg gttatattct ggttcccatt gacctggcat
    22921 tattcagctt ggcccaggga ttgcaaaaac ccgtttatcc gttattcaca ttgattttgc
    22981 ggatccttgt gttccgctat cctctggcgg tttattttgc cgctacctgg ggtgtgagga
    23041 gtgtttattg gtgtgaaccg gtttcaatgg gcgtagctgc aattttttcc gcatttcttt
    23101 tatggagact gaccaatggc ttgaaaagtg tagtttcccg ctattaattc cggtgtatta
    23161 taaagacgtt agaacgagcc aacaaacctg tcaggggata aatgataata cgactaacga
    23221 aggcaattgg attcaattta atcaaaatgc gtccgcgaag aagcaagggt ttcagatgaa
    23281 ggactgaaat cccttttgat tgcttgaaga aagacatgga ataagcacta aataggattt
    23341 tatctgcgtt tttaaataaa aaatatatgt ggtatattaa aatcatgaca gcaaaggggg
    23401 gatagcatgt gaacatgtgt gaaaaaggca gcataataga tgaattaaag aaatcccatc
    23461 cggagaagtt tgtttccgtc gcggaagtat tcaaaaatat tcgccgcggc aataaaatct
    23521 tcatcggcac cgccgccggc gaaccaaggc atctggtcag atctttgatg gattacctgg
    23581 tcaataaccc caaggcgttt tttgatgctg acatatatta tgtttggacg gttttcgatg
    23641 agagcaacta ttttttggaa aagtacaaac gcaattttag aatcagttcc ttttttatcg
    23701 gcgaccccaa tcgggatgcc attaacaacg gagacgctga ttttacgccg atatttactt
    23761 ccaggatccc ggaattaatt gatcggaaaa tgttgccggt tgatatcgcc ctgattcaag
    23821 tatctttgcc ggatgagcat ggttacgtta atctgggtgt cagcgttgat gttgccaaaa
    23881 cggcagtgga aaacgccggg ctggtcattg cccaggtgaa ttccttcatg cccaaggtgc
    23941 atggggatgg tttcatccac atcaatgata ttgactttat gcttcctttt gatgaaccac
    24001 tgccggaata cgatccccgg gaagaaagcg aagtcgtatc ccagatcggc aaatatgtcg
    24061 ctagtctggt acaggatggt gatacgattc agatcggcta tggaaatgtg cctttttcca
    24121 tattgcccag tctcagcgat aaaaaaaacc tgggcgtgca ttccgagcta ttaaccgacg
    24181 ggattattca attaatgaaa caaggcgtca ttgacaacag tcgaaaaacc atcaaccggg
    24241 gcaagaccat cggaacctac tgtctgggca agaaagaaac ctatgatttt ttaaacaata
    24301 atccggcggt agagttgaga aggattgatt atgtagataa tctgacgacg attgcccagc
    24361 acgacaatat gacggcgatt aatacggttt tgcagattga cctcaccggc caggcatcaa
    24421 cggaatcctt gggcacggtt tattacagcg gtattagcgg atttgccaat tttatgcggg
    24481 ctacccccct ggccaagcga ggcaagacga ttgtagcgat gaagtccacc gccaagaacg
    24541 ggacggtttc caagattgtg cccaatctgc tggaaggctc cggcgtgagt ttgcaccgtg
    24601 ccgatgtcca atatgtcgta accgaatacg gaattgctta tctgcatggg aagaatttac
    24661 gggaaagggc catggaattg atcgccattg ctcatcccga ttttagacca tggctgatcg
    24721 aagaggccaa aaagctgaat ctgatcttta aagaccagaa gtttgtaccc ggtccccgcg
    24781 gcaaatatat ggaagaatac gaagcgctca gagaaaccaa gaccggcctg gatatcctgt
    24841 tacggccgat tagaattagt gatgaacaaa gattaagaga tttcttcaat acgctttccg
    24901 acaagagcct gtatctaagg ttctttacgc cgcgcaaata tgttccccat gaaagcctgc
    24961 aggaaatcat cactgtggat aacgccaaag ggatttcgat tgttgcgata gaccaacagg
    25021 aggaaactga aaaaatcatc ggcttgggcc aatattttct taacgaaacc aactattcgg
    25081 cagaggtcag ttttgctacc agtgatgatt ataaaaacaa tggaatcgct tcgatattgc
    25141 tgacccattt gacttatgtc gcccagaaga acggtttgat tggctttact gccaccgttt
    25201 tggcggaaaa tcgttccatg gtcaaggtgc tgaagaaaat gggtttcact cacaagtcaa
    25261 ccgaagaagg cgtatccgat tttgaaaaat cattttaaat tctgaccggg ttcccaatac
    25321 ggagaagcaa ggcagtttta tacaataaac agcataatga agggaagaac aaagttaatc
    25381 cataaaatca acggaggaaa tatggcgttc tcaagatctt tggaaataaa aaccctgcag
    25441 caaatgatca ccatgtattg ccatgcccat catagcacct caaggaatga gttgtgtgcg
    25501 gattgcagcg cattgtttga gtatgccgga cagcggattg ataaatgctt ttacggcgac
    25561 gacaagccgg tgtgctcgca atgcactgtc cattgctata agccggaaat gagggaaaag
    25621 atcaaagctg tcatgcagta ttccggtccc aaaatgatgt ggaccagtcc catattgtcg
    25681 ctcaggtata tgtacaggaa gctatttaaa agcaatattt aaagaaccga tgtgacattc
    25741 cttcggcttt gactgaagga atttttttgt gtaatggggt tacaggtttt catttgacgt
    25801 tttattccca gacatttggg ttatcttttc cataattgtt ggttgacccg ccgatattgc
    25861 aggaattaaa tcggcgaacg agaattactc ttgtatgatt caaatagaca attaattccc
    25921 aatattgcat aaggggagtt gatgcaaacc acatgaaaaa gatgatactt tggcttgctg
    25981 ctttaatgct attgggtttc ggcatgatgc tgatggtcaa gatcccggcc ttgggtctgg
    26041 ctgaagcagg tttctgcggg aagtgccatg ccatggatta tcaggttagc acctatctgc
    26101 attcctccca tgcacgggag gccaattgcg gagattgtca tgacccgcac ggactggtca
    26161 ccggcagtat gtatgctgct tacactggca gcagggatgt ctatcgcgtg gtcaccaata
    26221 ccactccgga tgaaattcgc gccacggaac tgagtaaaaa gattctgcag gaaaattgtc
    26281 tgcgctgtca cggagacatc atgggagacg ttggcgatac cagggagaat ggaggcgtct
    26341 attgctttca ttgccatcag aacattgttc atcccaaata ggaaaggaga ggtgttgttt
    26401 ggaagcagca aagataaaaa aatgggtgtt ggcagtgagc gtcatcggta tggttggtat
    26461 tttaaccggg gttttctttt tcaatcgcaa taataccgtg ataacggaaa ccgcccgggg
    26521 cgagattgcc gctactgaga ctaattcggc tgtatggggc cagttttatc ctgctcattg
    26581 ggacagctat caggccaatc tgagcaacgc agcccagccg tctcattatg ataccaagct
    26641 ctacttgcag gccatgtatg aaggaaccgg ctttgccaag gaatacaacg agccccgtgg
    26701 acacatattc agcattgagg atgtgacatc cgttaatcca gcccgacaaa aggcgggtgc
    26761 atcctgcttt acctgtaaat ccacccaagt tcctgagatg atggacaagt acggggcgaa
    26821 atattatctg atgtcttttg acgaaatgaa tgggcaaatc acccaaccga tcggctgttt
    26881 ggattgtcat gatcccaaga ccatggcttt gagaatcacc cgcccggctt tgcaagaagc
    26941 cttgcagcgg caaggacggg atttggacaa ggtcaccaac caggaaatgc gcagcctggt
    27001 ctgtgcccag tgccatgtaa cctattactt cgccccggaa accaaaaaag ttacgttccc
    27061 gtgggataaa ggctttacgg cacaacagat cctggcttat tacgatgaag aacaatttac
    27121 cgaatgggtc catcccgtcg ctggatcgaa tttggtgaaa gcccgtcatg cggaatatga
    27181 aacctttatg ggcagcacgc atcaaacagc cggagtggcc tgcgctgatt gtcatatgcc
    27241 ttatacggtg gtgggcaaca agaagatctc ttcccatgtc tggcagagtc cgctgaacaa
    27301 cattgaacag agctgtacca cctgtcaccg caatggcacg gaatggttaa aaaaccgggt
    27361 ggaaaccatc cagacccagg ccaaacagag tcaggacttg gccggagaag cagtagtaca
    27421 ggctatcaac gaactgaaaa tggcccggga aacacctggt gttgatcagg ctaagctggc
    27481 cgaggccatg aaactccatc gtgaggccca atggtatctg gattacgttg ccgtgaccaa
    27541 tggctacggt ttccataacc ccacggctac tttgaacgac ataaatgctg ctctggacaa
    27601 agcacataaa gcagttgtga aggcgcggga agccaggggg gacaacaatc cttcttgaga
    27661 agtgttaggt atgagcagaa tatcattaac ctttaatcaa ggtaaacatg aaatgagggt
    27721 tggaaatgga cagactgaag gacttggggc gtatcaaagt gatactggga gagaatagcg
    27781 gacgcttccc cttctgcaat tccatcctga tcgatgattc tgtgcaggcg gtgatcgatc
    27841 cgggggcggg cttgcagcaa ttgcgggcga taaatgaccg gcagaatatc gaagttgttt
    27901 tcaataccca cgttcatttt gatcatatcg ttttcaatta tgtcttcgat cattcccgga
    27961 tcatggtcaa cgaaccggaa tccatctatt ttcgcgaccg acgcaaattc attgaggatt
    28021 tcggaacgag tcaggtctgg ggtgatgcct gggccgatca ttggctggaa cagactgcgc
    28081 gaccggagaa cctgcagagt aaaaccattg cctatcggca tgaatggtac ctttccatgg
    28141 cccgtttgga tggtacctac cgctgggggg acgtttttga ttttggccag gtcaaaatgg
    28201 aagtgattcc tgcccccggg cattcgcagg gtttcaatgt catgtatttt cccctccagg
    28261 gaattgttta ctgcagtgat attgatttaa ctgcttttgg cccttggggg gaagattacg
    28321 atcagttcgt cgaatcagcc cggcgtattg cccaactgga tgcagatact tttgtaaccg
    28381 gtcatgaaag cggtatcgtt tccaaaaacg aatttgtttc ccgcctggat cagtacctgg
    28441 aactgattga tcgccgggac gatatcttgc tggagaagct gagccggcca aggacgcagg
    28501 aagacctcat taaagaaggc actttctatg gcccccggat cttcagggat gaatttgttt
    28561 attgctggga atggtatatg gtcaaacaac atttgcgaag gctgctcaaa caacatcgga
    28621 tagtcgagaa agaaggaatt tatagccgcg tttgagattt ttgaatgtag ataaaatata
    28681 taacattttt attgagagaa aaattgatgg agatttttct cttaattttt taggaaaaca
    28741 atttcgttga ccgaggtcat tggctaaagt acaatggaat caaaccctga aagaataata
    28801 aaaaggaggg attttaaacc gggcttgatt ctgtttgatg cacataatcc ccgttacaga
    28861 gatcagttgc ataatattcg tttcccaggg agattctcaa aatagcagaa gcctttaaat
    28921 tacacaaaat acaggaacgc ataaaaggag gaaatgatga tggattttat cggcaaacaa
    28981 ccaggcaaaa catcgttgac cagtaacggc atctctacca tcagaggtca tgtgcaaaaa
    29041 catcctgacc gaatcgccct gaaaacggag gattttagtg aaacctacgc cgaaatgtgg
    29101 cagcgctgtg tgcgattggc caatgcgctt ttggccaaag gattgaacaa gcccgatttg
    29161 atagcaacct atatgcccaa ttcctaccag tttgtcgaag ttatcgtggc tgcggagatg
    29221 attggtctgc ccataacctt gggaaattat cgtctgaccc cggaggagat tatttatcag
    29281 atcaataact gtgaggccaa agtgatcttt gtcaaacagg aacaatatga tttaatttct
    29341 ccattgctgg atcgcttgcc caccattaaa gaaatcgttc tgatcagtga taccccggca
    29401 gaaggcgtgc tgaactacga agaactgatt gccggcggga gtcaagagga acctaaagca
    29461 gatgtgttgc cggaagacat gcacctgctt ttctatactt ccggtaccac tggaaaaccc
    29521 aagggagcag tgcggaccat gtactgtgat tacaatatgg ctatctccac tgccatagag
    29581 cttggtctga gccgtgatga ttccatgctg gtcgttgccc ccatgtatgc cgcggcgact
    29641 accggttatt ttctcaccac gttaatggta ggcggaacgc tctgtattgc gccggctttt
    29701 ataccggagg attccctgcg gctgattgac ctgcataaac ccagttttgt ttttatggta
    29761 ccgattatgt ttgactggat gctgtccctg ccgcctgaaa ccattgccaa atacaacctt
    29821 tcttcggtac gattggcagt agcctgcgga gcccccatgc acagcaccat ttttcaaaaa
    29881 atggcagatc actttaccaa tgccaaatgc attaacatgc tgggtgcttc tgaattgggt
    29941 tttgtaacga gcattacgac agaggaatgg ttcggactca aaaaggaagg cagtattggc
    30001 aaaggtattt ttgatatgga tttgaaaatc gttgacgaca aaggtcagga agttggtccg
    30061 gacggagtag gggtgcttta ttcacggagt cccgctacct ttgatggata ttggcagaac
    30121 ccggagagca cccgggaatc attcctgaat catgaatgga cctcggtggg agatatggcc
    30181 cggattgatg aagatggcta tatttatctg gttgaccgcg ccaaagatat gattgtcagt
    30241 ggcggcacca atatttatcc ggctgaagta gagggcgtaa tcctgaaatt gaatgggatt
    30301 gctgatgtag gtgttattgg cataccggat gaaaaatggg gcgagcaagt caaagccatc
    30361 gtggttttga aaccgggtta ttccatcagc gaagatgaga ttattgccca ttgccggcag
    30421 catctggctg gatttaaggt ccccaaatca gttgattatg ccgatgccat cccccgcaat
    30481 gcggtaggca aaatgctcaa aaaggaactg cgcaagccct actgggaagg gaaaaaatca
    30541 tttatatcct gattccttta caagctaaca tgaaatcaac ggctagagca actgcggcgg
    30601 attaagcgcc gcagttgaat ttttgcttaa gtttatgttg gctgaggaaa aatggtataa
    30661 tagacccgcc gaaactgtta ttcaaggagt cgaacatgaa tcaaaagaaa cctaccaaac
    30721 gcgacctgca ggcggcagaa accagaaaga agatatttga cacggctttc cggctaatca
    30781 ctgaaaaagg ttttgaaaat gttacggtag aagagattgc caaagaatca gaggtagcca
    30841 aaggcctttt ttatcattat tttaaatcca aagatgacat tgtggtggaa acctatttca
    30901 ttattgataa aagctacagc caattttatg cagagaatgc gccccattgc gatgctattg
    30961 aaaaaatcct gctgacggtc gttttccagg cgcgtcatgc caccttcctg ggggttgatt
    31021 ttgttcgtca aatatacaaa agtcaactct atgccggcac gcagtatttt atttccaagg
    31081 aacggtcctt ttatacaatt ctgcgggatg caatccaggc tgcgcagata gagggtacga
    31141 tccgtgacga cgtctcagcc gagaaattag ccaatatcgt acttagctgc ggacgaggca
    31201 tgctttatga ttggtgttta aatgacggca aatataatct ggaagagcgg atgaccaacc
    31261 aattgaccat aattattgaa ggtatgcaaa aaagataaat aatcaatgaa gaagggcctg
    31321 cttatggcgc ggcacagtct tcatgaaaga tttgttatcg ataccggcct tggtcataga
    31381 gaaatatcgt actcgccaaa ggctgggtgg tacgctatca tgatgactct tgcaacggat
    31441 tttacacaca aaaaagccgt ctgccatgaa gcggacggtt aggctttcag cctccttttt
    31501 gggaacgaaa cgtgcagctt tgacgacggg gcttccattc tttcaatgtg cgcgttacat
    31561 gtctttttat gctggtgctt aagattttac tgcggcattt aagaaatgtg ttcaagaata
    31621 tatgggagaa ccttatccgg agatatattt aatgcaatag caaattcttc atgcaattgt
    31681 ttttcggctg ctcgcatgat ttcttcatct gtactggtca gggttttgcc gttggccaag
    31741 cgggcttctt tttccacatg cagggttttg atgagtttta tccattcctg gcacgttcct
    31801 cttttgagaa tggttttata taactcattt ctttcccggt tgtcttctat ccaaatggtt
    31861 tcctgggttg gcatggcggc gattagggac aggacttctt cttgggtaag gattggcctc
    31921 atggcaattt tgggattatt tacaggtatt tttatcgtca tattggtatc ataaacaggt
    31981 tgcaaaacgt agtactcggt atcattgttg ccggcatatt catcttttcc gatagcggtg
    32041 atccggcata ctcccgttgt gctatatacg acataatcgc ttaccttgaa catgggtgtt
    32101 cctccttgca gtaagattac gaacagatca gcagataagt tatctgcgaa aaaaagaaag
    32161 tctcgacaac aggatttacg ccatcgagac aacacgttgt atttcaatta tagcaaaata
    32221 atcaagtttg atgtaattgg caaaatacgt atattctcaa caatgcttgt tgtaccttcg
    32281 ctgcggccat tattctctgg gcgaatcagc gcttcacccc cgatgacagc aaatgaaaaa
    32341 gaatttcatt tggttttagg cttggtaaat tttgaccatt cttcggttat ctttgcgccg
    32401 cccaatcggg tccgcacttt ttttatctgg aatgaagttc cctcgtattg accgtttacc
    32461 actttacatt ttaaattggt gcgcagcatg tcgccttttt tattctcgct gtcgacgtaa
    32521 aacaaaatca agtaactgtt gtcatccaac ggctcgatca tgtctacaga aaaatcggga
    32581 tatacagctg agttaggggc gatcagctgc ttggtggtgt ttttcttgca agtattgtag
    32641 atgcttttat attctgcgac aatctccccc tcatccgcca attcggctaa aagcgcaaca
    32701 attttgtcat gttccatggg ggtaatgatt ttacgatgga ggagttcttt tattttggtt
    32761 cgctttactt ccaatgacac gatgatcacc tctttagcag atgtacaggt ttattatatc
    32821 ataattggca tacaaaactg gttccgttca gcatttcttt tacatccatt aaaaaattgc
    32881 ggataattct ttagagttag gggggagatt ggcaatacat gttggaggat acaagagatg
    32941 cgtatgaaca aggaaaataa ctacaaccaa accatggaca aagcaattac aaatgaaaca
    33001 agctgttatt gcctgcaaat gggttcaaag tattgtaaaa aacgaatgtc ccaaccaatg
    33061 ctatggtcgc tttcaggatt tgattgacaa actgttcgag gatgccatga aaataaatac
    33121 cgacgcccag gagtgcccat aaagggttaa agtttggtgg ttcgaccggt ttcatgtaaa
    33181 tcatagtcac ccagaccttt gagttcatct gcaaattcac tggaattgat gatttgcaaa
    33241 acggctttgt accagtcggt ataacaatct tcctgcttca ccaccagatc atatctttca
    33301 ctctgcaagg ggataaattc aatttcccgt acctgcatgg ccgccttctg gttgcccaga
    33361 cccacatcag cctcgcctcg ggcgacggca ctggcaaccg ccaggtggct gaactcttct
    33421 tgtgtatagc cttgtatttg caaacgatca tatcccaaag aacgaaattt ttcatccaat
    33481 agaattcgtg tgccgctgcc tttttctcgg tttatgaagc gaatctccgg gcggacaaga
    33541 tcggtccaat cctggatgtt tttagggttg cctttcttga cgtaaaaacc ctgcatacgc
    33601 attgccaaat gaaccaggac aaccgggatg ccgggcagca aacggcgtac agaagcaaca
    33661 ttgtatgttc cggagtcacc atcccagagg tggactgccg ccatatcagc acgattatga
    33721 taaagtgcca tcagaccatc aaagctgccg atttgattgc gcagtatttt aagtcccccg
    33781 ctgtatcgtt ccatgtgtcg ggcaagaatg tccagaatga tgtcctgtcc gcaaatcacc
    33841 agttcctgcc gacgctctgc tccggcatag gaaaaacagg tacctttgac ggtcggttga
    33901 gtttgattga ccggaccaga gggttgcgta aatttgcccg tttgtttata catatccacg
    33961 tcatgctggt caaatctgag ttttctgccg atcctaaagg ctggaagatc tcctcgtttc
    34021 accaattcgt aaacggtatt cttggttatc tgcaattgtt gggctacttc ttgtggggtc
    34081 agagaattat cttgatgggt atgcatatta tcacctcatt caataaccat aattgaattt
    34141 tatcacatat ttttttactt ttgcagacaa agca
//
LOCUS       DAOTHZ010000016        32826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1505_length_32826_cov_7.882152, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000016 DAOTHZ010000000
VERSION     DAOTHZ010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 32826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32826
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_32826_cov_7.882152"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1919
                     /locus_tag="P5549_03395"
     CDS             <1..1919
                     /locus_tag="P5549_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRW12151.1"
                     /translation="ETLAANKLVYTLSLSYLEGNDQEKIVAQLLTLLQPYYPEVTAAQ
                     IKEKIELQKYRLYEPVAIMRDIPWELVVKLEESRQDLPGVAVNIEPLRVYPQQHLAGH
                     VLGYIHSINEEELAASGEKYSINSLIGKSGVEKEYESQLKGKDGARRVEVDAQGRPMG
                     ELVTMSSVPGKNLTLTLDLKLQKVMESSMESNLQRIQQTHPKAKVGSAVLLEVKTGEV
                     LAMASSPYMRPDDFKGKLASSVAAYYFPAGDQYDPMEPGAAINRAIQAQYPPGSTFKP
                     ITGMAALESGNLDPNDYINCPGRYWIAPYITCTGVHGNENYFSGMADSCNTYFQEAGR
                     RAGKDKIIEVAKDFGLGQKSGIDLSGEASGLVPTPAWKKEINAILIDRKYDHLRQQLT
                     EKYDRLLAAAQSDEEKEELQQQQKNESAKLEAQYKIDYNFDTQWQAFDTFNMSIGQGS
                     NQYTVLQLANYVAAIANGGYLMKPHVVKEITDPNSEQVELVKPNIIHKTSVSSSTLAL
                     TRQAMQAVTGAGGTGSFLFTNFPATLPVAAKTGTAQTGRVGDKTLSEFHGVFVAYAPA
                     NDPQVAFAGVIEYGQHGSESAGWVAKAVFEQYFGLVDHYQEIIDAEEKTRSAQAKKDG
                     DAITRPAGNTTSQE"
     gene            2329..2751
                     /gene="minC"
                     /locus_tag="P5549_03400"
     CDS             2329..2751
                     /gene="minC"
                     /locus_tag="P5549_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRW12152.1"
                     /translation="MKELINEKDPRSDPDRQCFYQGFNEGENTVSNNTTLIYRSLRSG
                     QKYRSEGNMVILGDINPGAEVIAGGNILVMGALKGIAHAGANGDENAVIAAFRLNPIQ
                     LRIANHITRPPDGECIIVNTPELAKIRAGKVVIEKLKI"
     gene            2767..3567
                     /gene="minD"
                     /locus_tag="P5549_03405"
     CDS             2767..3567
                     /gene="minD"
                     /locus_tag="P5549_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRW12153.1"
                     /translation="MKSKVLVITSGKGGVGKTTTTANLGTALAMLGKKVVMLDTDIGL
                     RNLDVVMGLENRIVFDIVDVVNGNCKIKQALIKDKRFEGLCLLPAAQTKDKTAVTPHQ
                     MKNLTNELRKEFDFILVDCPAGIEQGFKNAISGADDAVVVATPEISSVRDADRIIGLL
                     EAAGLSNSKLIINRMRSKMVKRGDMMDIDDILDILSINLLGVVPEDEAIVVSTNRGEP
                     AVLETTSRAGEAYRRIARRITGEDLPIDSQEDSINLVDRFKRILKLAR"
     gene            3581..3853
                     /gene="minE"
                     /locus_tag="P5549_03410"
     CDS             3581..3853
                     /gene="minE"
                     /locus_tag="P5549_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRW12154.1"
                     /translation="MLELISRIFGKETSSSKDIARERLRLVLVHDRSTVSPEIINLLR
                     DDLIQVIQKYMDIDMEGLLVNIEGESNSVALIANIPIKGLKRAPAI"
     gene            4114..5250
                     /gene="rodA"
                     /locus_tag="P5549_03415"
     CDS             4114..5250
                     /gene="rodA"
                     /locus_tag="P5549_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRW12155.1"
                     /translation="MQIKRIKFLDKGFLFSLAIILAFGLVILFSASSGITSDPFYYVK
                     KQILWLIVGLVMAIMLIRFDYSQLEGYWKYLYGISIITLVIVLIFGQEVRGTTGWIGF
                     GPFPKIQPAEFTKILLILAFAAYLNKRKDSLDTFREMLPCFIFMGIPFLLIMMQPDLG
                     TALVYIAVTLVMMFIAGANPKVLGMIILIASLFIALMLFMHFKFDTWMPLDDYQIKRL
                     IVFVDPYNDGQGGRDAGWNTIQSLVAIGSGGLTGKGLFNGSQVQLNFLPEHHTDFIYA
                     VIGEEMGFIGAVLVILLYGILLIRAVIISFNANDLYGSFLVIGITAMWMFHIFENIGM
                     SIGIMPITGIPLPFLSYGGSAMLTNMLAVGFILSVNMRGKKLVF"
     gene            5272..5718
                     /locus_tag="P5549_03420"
     CDS             5272..5718
                     /locus_tag="P5549_03420"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRW12156.1"
                     /translation="MKKEGIDLDMARGKLQEAIMLIPNYLKLLYRLTRDNRVQVTEKA
                     LLLGTAAYVLSPIDFLPDFIPFVGQVDDVLLVALILKRFMNSVSPQVLYQHWDGRPDL
                     LRNIEQILSWSRFFLPADVYQKVVRKSRENPGHGSHDTIDADYEIK"
     gene            5822..6397
                     /locus_tag="P5549_03425"
     CDS             5822..6397
                     /locus_tag="P5549_03425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRW12157.1"
                     /translation="MFYSFKIRLLMVIVLAAVLGLVMQSDSPGKQAVEPVLRYIMTSD
                     DALTQTFNQLIYHPERSGLESLPASVRTLIKSPCEYIAVEQKFGSYWNEAQGGETFNP
                     GMNFKVAASTPVKAVLPGTVQAITKESREYTVKIKHIGGLESIYGGLREVEVKVDHPV
                     LIEEVIGKTGEQFYFEMRSKNDPVDPRSIFE"
     gene            6430..7257
                     /locus_tag="P5549_03430"
     CDS             6430..7257
                     /locus_tag="P5549_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRW12158.1"
                     /translation="MRINWLFLLVCAVYTWLGLGSEILIIFASVLLHELSHTIMAKIL
                     RVQVAEVELLPFGGQAKIEDFTGLDPDREIYIALAGPIMSLSLAAFFYLLPPHLLHKT
                     PLLLQINLFLGLFNLLPALPLDGGRILRAILSLRIGYKRATSVAAGLGKTIAILLCAY
                     GGYLFYLQQSGANHILAGLILFWAARREGLLLGYAFMRYLVNKKSELANKGFLASHQV
                     VSFEDTLIKSILASTRPGYYTMVMVLDQQHMPQGLISEAQMIESLLNHGPRVRLKDC"
     gene            complement(7326..7613)
                     /locus_tag="P5549_03435"
     CDS             complement(7326..7613)
                     /locus_tag="P5549_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12159.1"
                     /translation="MPSKDTEQFIKQLEAELAAKNNEIAALKAREAQVIKSNQAQAEE
                     LEDLRVLALAVRKHFHNNLAVERCAACSKCDYEELEKAMLQCKAFTRIRLD"
     gene            complement(7732..8502)
                     /locus_tag="P5549_03440"
     CDS             complement(7732..8502)
                     /locus_tag="P5549_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12160.1"
                     /translation="MSNSKLKMFTKLYLKDLHEVTAEILIVVIGVVLVTIGFSLDSEV
                     PPPAFLFPLFLLLGLVGFLPLLSSFKLLSREWSHNTVYLVMSLPVSGAMILASKMMVL
                     LTQYVGGTLLLGLSGYLLFATKLFQYIAPYQPDLAVLANNPELVSNLLAFYLAGLAFL
                     VFLCCTSFFSQIAGRLSAKFSGLVTAVTFVFTLILSGKLLEALGTGSHEVNLVMVRMS
                     GNTDSLVNSLYLSSLGYLLLAIILFILAAVIYDRKLEL"
     gene            complement(8489..9187)
                     /locus_tag="P5549_03445"
     CDS             complement(8489..9187)
                     /locus_tag="P5549_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12161.1"
                     /translation="MIEFDQVGKRFGSIQALDQLSLQFGPGKIIGLFGPNGAGKSTAM
                     KMIAGLNQPDSGHVLVDGQKPRHSRVKIAFLPEIDHLYGWWTLKQAADFMRSFYADWD
                     DRKYTELLGFLNLREDMKISKISKGQRAKCKLILTVSRRAPYLLLDEPLSGIDILTRE
                     EIINTLIRDFREGEQSIIISTHEINDVESLVDEVFFIDQGRIVVSGSAEELRITQGLS
                     LVEIMKEAFRHVQQ"
     gene            complement(9180..9569)
                     /locus_tag="P5549_03450"
     CDS             complement(9180..9569)
                     /locus_tag="P5549_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRW12162.1"
                     /translation="MSQLDYDTSKAIYKQIIEDFQKQLVRGELGPGDKIPSQREYALK
                     VSVNPNTVQRAYQELERIGLAETIRGQGTFVKADTTLISEIRQAMADDILNRFFAEMN
                     SLGYAKEQITEVLNRRLMEMKGATTND"
     gene            9724..11463
                     /locus_tag="P5549_03455"
     CDS             9724..11463
                     /locus_tag="P5549_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12163.1"
                     /translation="MKKHTIKPLRQPDRIINYWKNDKPVAAFIIVFGLGYNIAAILGP
                     IYQGKLIDALLRGDGLAALIRLAVFFVLMIAGIQLMRYLKRFYTRRFANQTGAVMRLM
                     IYNSVINKSYLELQHENIGDLMTRVLADVDLCVEGMRKFTTEVFDTGVLMASYLAAML
                     YYDVKITLLGAIFIPVAMIIAEKLKRIIYKYSIAYRTKSSIITGMTYEYIEHAMLYRI
                     NGLERQSREKYHQELNDLQDQAIKANLFGNAMQPVYNVVAMLGVIIVIYLGGNRVIEG
                     SWTVGNFSAYMIMFAALATKASKAANLFNSVQKSQISWQRIKPYFREYQSQDTAQDIR
                     QGLTSLTIEDMSFHYPEAQENVIKSISLNARQGEIIGVTGAIASGKTTLGMSLLGLYP
                     YAGSICIDGKELRDYSDYERSQMIAYLGHNPQLLSDTIYNNITLGGNEDISEVLHDVC
                     LDEDLPAMPQGADTMVGSSGIRLSGGQQARIALARTLLHKAKIIILDDPFSAVDMGTE
                     EKIIRSLKNNYQQCLIILISHRLAIFSQIDRIVLLHPDHSFDYGTHHQLMETSELYAS
                     IYELQRLAGEQNE"
     gene            11456..13201
                     /locus_tag="P5549_03460"
     CDS             11456..13201
                     /locus_tag="P5549_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12164.1"
                     /translation="MNKSVVKKSFCFTAKNNKMIMVLLILVVLGAVTASLIPPQILRL
                     VIDQNLVPKNPNGLIRLAAFYLAVLVLVGIFDFIKEFILTVMGQKVSKEIRLAMMAKL
                     ARVNAGYFSSQDSGALVSRFTNDVDAINTLFASGVAGMLVSVFKIVGIVVSIWLFSYK
                     LGLFTLLFLPVIYFITRLFQKRMLIAQIKNRVLVGKVNNHIAESLNNVLMIKAGNKEE
                     YMEQNYTGYLRENFQTLEKVNFYDAVFSPLIQVLRAFVIASIVIMSANQLGWLGISLG
                     MVAASIELISNLFEPVENFGMELQNIQQAVSGIRRVDEFYGEPEETGKNEELTAETVI
                     PDRACVRLAFRNVNFAYEAGNEVLQNIDLSIKSRQNVTFAGRTGVGKSTLFKLVLGLL
                     QPQEGVITINDIDVCSIPHHEKRKIFGYVEQGFHLIDGSVADQISLQDAHISRTQIEQ
                     ALELVGMREYVEQLENGLDTEVKGDTQFSQGQKQLLAIARAIVTDPPILLLDEITAHL
                     DSLTEERIVAVMRKAGQARTILSISHRLSSLVASDIVVILENGRIKNAGSPEMLLQDD
                     DWYRSHIALEKLTWK"
     assembly_gap    13326..13425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13510..14655
                     /locus_tag="P5549_03465"
     CDS             13510..14655
                     /locus_tag="P5549_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRW12165.1"
                     /translation="MIDNLNKAPRNQTVKLIILAGMVLLLVMYFPAVINKTKALLAVF
                     YPLLLGAFIAFILNILMAGYEGIYFPASRNRFINATRRGMCLILAILTIVLIIYFVLH
                     IVIPQVGHTIALIAAGFPEAYNDALVWLQQYSDEIPGLQQRLAALDMDGSAILQQALA
                     LLGGWAFGTVSMIGSVFGKMVNFVLALVFCIYILLDKENLKVKFDKLFRAYLRYDRRE
                     KLYADLRVTNRTFSDYIVGQCKEAVILGVLCALGMWIFGFPYALSVGSVIGLTALIPM
                     IGAYIGAAVGFLLIVIVDPLKALLFILFIVVLQQLEGNIIYPKVVGNSIGLPGIWVLA
                     AITVGGGLMGITGVLLGVPVAATIYKLLGQAVNARLDKNAPIADESM"
     gene            14935..16767
                     /locus_tag="P5549_03470"
     CDS             14935..16767
                     /locus_tag="P5549_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRW12166.1"
                     /translation="MKERLFRDILPRVEKPARYTGSEVNMIKKDWDSKSAKMVMAFPD
                     VYEIGMSHIGCKILYGLVNETTNHLMERSFAPWPDMEALLRQEEIPLYSLESFTPLAK
                     FDVVGFSLQYELSITNVLNMLELGGIPLCSAQRSDTDPLVIAGGPVAFNPEPFADFFD
                     AFLLGDGEEVTVEFLDCVETNRHLEKAALLQELAQIEGVYIPALYEVSYHADGTIKSF
                     APSAEGIPLRVRKRLVRDLDLAYYPSQPVVPYLGIVHDRAVLEVMRGCQRACRFCHAG
                     MVYRPVRERSVEVLARQAEEQLKNTGYDEISLASLSTLDYSGVEILVKQLVEEYGRQG
                     IGVSLPSLRVDAFSIELANEVQKVRKTTLTLAPEAGTQRLRDVINKNVTEEDLLVAAE
                     AVFKSGWMGLKLYFMIGLPTESEEDLQGILELLKKVKSIGSRYSRRAIEIRASLACFV
                     PKAHTPFQWQPQAGISSLEEKRHFMLKQKMKNVKLSFHDSRTSYLEGVMARGDRKLGE
                     VIHRAWRMGCKFDGWSEYFSFTTWREAFAECGIDPDFYVRERSRDEIFPWDFIDIGVD
                     KDYLWEENERAFQRILTPDCRQDGCVSCGVCPQFEFEPFSGEGA"
     gene            16769..17440
                     /locus_tag="P5549_03475"
     CDS             16769..17440
                     /locus_tag="P5549_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRW12167.1"
                     /translation="MRLRVEYSVGPELKFLANLDMMHLMERALRRAGIPYYLSEGFNP
                     HIRLSMGTVLPVGIWGKHEYFDLDLAEMDREDFIYNMNLALPPALSIMDCQQIEENAP
                     SLMKIINAAEYTFVIEAGLDLSDLTTRIMQQDTLPVQSRGKNKNVIKDLRPGIYQIRI
                     DRDEAYDLLSLMVSINEPLNIRYDELQALFIEYGLFAKNIADIFRQGNYIKEDQQFYS
                     PLKRV"
     gene            17444..18868
                     /locus_tag="P5549_03480"
     CDS             17444..18868
                     /locus_tag="P5549_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRW12168.1"
                     /translation="MVRDIIGEIYPWESRVAIVEDDRLVEVFWADQNESVGNIYKGKV
                     KDVLPGLSCAFIDIGLSKNAFLYVADVITAGSRKDKNIGQLLKSGQEIMVQVKKEAFS
                     EKGARVTCDITIPGHLLVLLPFQREVSISRKIIGNQKRNLLRSLVEENKPEDIGVILR
                     TACMEARDSEILAELNHLLGTWQDILKRFNQQNAPSLVYEDIDVIERALRDYLDADIR
                     RVVTNNLKIKEKIDLIALKKNTSFVPSVHFEEGDLFEKYGLEKDIRKALRRKVWLKSG
                     GYLIFDVTEAMTVIDVNSGKFTGKDNFEETVYKLNLEAALEIPRQLRLRSIGGIVLID
                     FIDMQDKNNEDNVISILRQELEKDKAHNRIMGMTQLGFLEMTRKKSRYGISEIFTDEC
                     ITCHGRGRTINLFALACEAKRKLANMGYLESEEIVCEAHPQLLQYLINEENNMNYIRK
                     KTGKKIRLEENSSMNIEEYNLYST"
     misc_feature    18913..18991
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19002..19313
                     /gene="rplU"
                     /locus_tag="P5549_03485"
     CDS             19002..19313
                     /gene="rplU"
                     /locus_tag="P5549_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRW12169.1"
                     /translation="MYAIIETGGKQYKVSEGLMIKVEKLSCEAGDKVTLDQVLMLSDD
                     NGTRIGNPLVEGAKVTAAVLEQGRGKKIIVYKYKKRKNERKKQGHRQAYTKLLIEKIE
                     G"
     gene            19375..19647
                     /gene="rpmA"
                     /locus_tag="P5549_03490"
     CDS             19375..19647
                     /gene="rpmA"
                     /locus_tag="P5549_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRW12170.1"
                     /translation="MAHKKGVGSTRNGRDSESKRLGVKRYDGQVVRAGNILVRQRGTK
                     IHPGNNVMIGGDDTLFAVVDGKVKFERKDKTRKQVSVYPVDESATV"
     gene            20036..20488
                     /locus_tag="P5549_03495"
     CDS             20036..20488
                     /locus_tag="P5549_03495"
                     /inference="COORDINATES: protein motif:HMM:NF026040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HRW12171.1"
                     /translation="MEAGQIVSMLRRVRHEYANHLQVISGYLDLGWEGQLGSYVRAII
                     TELNQERIIFDSLPPEEALYFYEQLLMVKDLGIILVYDDLDVSSTGYLRESNEPVNSI
                     AALQSDIQANDEDPVIYLSIHENEGGIDLYFCCESWEGESRHIHIDKR"
     gene            20493..21755
                     /gene="obgE"
                     /locus_tag="P5549_03500"
     CDS             20493..21755
                     /gene="obgE"
                     /locus_tag="P5549_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRW12172.1"
                     /translation="MFIDHAKIYVKGGDGGNGCVAFRREKYVPMGGPYGGDGGRGGNV
                     IFVAEEGMTTLMDFRYRKHFKAERGNHGLGKNQHGAWGEDLIIKVPMGTIIRDDDTGE
                     LLADLTEHGQQAVIAKGGRGGKGNSHFANATHKAPSMAEKGEIGQERWINMELKLLAD
                     VGLVGYPNAGKSTLIAQVSAARPKIADYPFTTLIPNLGVVGTNHHESFVIADIPGLIE
                     GAHEGVGLGHDFLRHIERTRVLLFILDAAETDGRDVQEDLRILQQELQLFNPELAKRP
                     YLIVANKMDIPGADERFAALKKVHGDKVLAISAASGQGVSELTEKTYALLQSIPRQEA
                     PPEEVRVIRRYEDEPPFQVEVVNGIYQVSGKRIENLVNMTNMEQEESLQKFQRTIERM
                     GLEDTLREMGIKEGDLVRIADLEFEYSE"
     gene            21804..22931
                     /gene="proB"
                     /locus_tag="P5549_03505"
     CDS             21804..22931
                     /gene="proB"
                     /locus_tag="P5549_03505"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRW12173.1"
                     /translation="MSVRGEFKQIKRLVVKVGTSTLIHANGQLNLERMERLVRELADL
                     HNRDIEVVLVSSGAVGTGANRMNMRKIPKTLPEKQALAAIGQGRLLHMYEKFFADYGK
                     TVAQVLLTREDLDERMRYLNATNTLLAILNMQVIPIINENDTVVVEEIKIGDNDTLST
                     MVAGIVNADLLIILSDVDGFYDNDPRTHRNARLQSEIYEVTEEMEAHAVARGTTFASG
                     GMLTKLKSARNCMAAGIPMVIANSAAENVIRRIVAGEPLGTVFVPREEKMPSRKKWLA
                     FGSAEHGQIQVDHGAEGALLNRGKSLLASGVTAVEGEFERGTVIGVINHAGKEIARGL
                     TNYNSIEIRQIAGHKSVDIEKILGAKDYDEVIHRDNLWVKC"
     gene            22949..24208
                     /locus_tag="P5549_03510"
     CDS             22949..24208
                     /locus_tag="P5549_03510"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRW12174.1"
                     /translation="MDRELELLLLEQGRRAKQASRFLATAGTELKNTALFKMAEQLEK
                     QSQEIIAANQIDLDQGERQGLTKALLERLMLNQTRIQDMTQGLREIAALPDPVGEMLE
                     MHHRPNGLDIGRIRTPIGVIGIIYESRPNVTADAAGLCLKSGNAIILRGGEEALYSNR
                     VIARILADAAAECGLPAGTIQLVDSEDREAAVFLMKMHDYLDVLIPRGGKGLKKAVLE
                     NATVPVIMTGMGNCHVYLDAFADYEKALPIVHNAKVQRPSVCNAAETLLVHSRVAEAF
                     LPGIIADLQSAGVEIRGCAQTCSIVPDLTAATEDDWATEYLDLILAVKVVDCLEDAIE
                     HINQYGTGHSEAIVSEHYPSVRKFMAGVDAAAVYANASTRFTDGSVFGFGAEMGISTQ
                     KLHARGPMGLQELTTTKFVIYGDGQIR"
     gene            24294..24887
                     /locus_tag="P5549_03515"
     CDS             24294..24887
                     /locus_tag="P5549_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRW12175.1"
                     /translation="MKSLKGTKTAENLLKAFAGESQARNRYTFYASVADKEGYKQIKN
                     IFIETADNEKEHGKRFYKFLLEGLAGELPAFIEINAGYPVAQGTTLENLQAAADGEYE
                     EWHDLYPEFAKVAEEEGFPQVAAAFKMIALAEVRHDTRFKKLAANIENDKVFKKEAKT
                     LWKCGNCGYVHEGGEAPKICPACIHPQEYFEVFTEPY"
     gene            complement(25037..25498)
                     /locus_tag="P5549_03520"
     CDS             complement(25037..25498)
                     /locus_tag="P5549_03520"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRW12176.1"
                     /translation="MKPQKTVKEYRNNDIIVYWYPQECSHAGKCWQELPEVFDMDKRP
                     WVNISGTSPERIIATIDKCPSRSLQYSLPEGSSVDPNLAKGPGSKDYKIDPEKAGRIR
                     VIREGPLLVEAPVRIIDIDGTIIGEGDRFVLCRCGKTKNPPFCDGSHIPGK"
     gene            complement(25526..25681)
                     /locus_tag="P5549_03525"
                     /pseudo
     CDS             complement(25526..25681)
                     /locus_tag="P5549_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618291.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
     gene            complement(25734..26768)
                     /locus_tag="P5549_03530"
     CDS             complement(25734..26768)
                     /locus_tag="P5549_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HRW12177.1"
                     /translation="MDWNRNKTIANGVLEAIGYTPMIRLNRWSQDLGTDICVKLEYVN
                     PSGSLKDRVVHKIVEKAEQRGDLKPDMILLEGTTGNTGIATAMVGAAKGYQVIIVMPE
                     GMSEERKKTIQSYGAQLVLTPGAETDVDLVIEELERIKARYGEKIFEIGQFVRQDNLL
                     AHYEHTGPEIWEQTGGKLDVFIMNQGTGGTVTGTAKYLKEKKPAIKIYISEPAESPIL
                     AYGKIGKHKVQGVADGLIPEILDLQYIDGIILVSSEQSVATAQEIARTEGIFCGISSG
                     CNLAAARMVAEKYPDEKMIVTIINDNGLRYLSTSLCGCETPRQELDRVYALDQKDLEK
                     LASYHLEIIE"
     gene            complement(26823..27443)
                     /locus_tag="P5549_03535"
     CDS             complement(26823..27443)
                     /locus_tag="P5549_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12178.1"
                     /translation="MVKEITLIFHNLGLRTYIEVDLCAECPRQDDKGCCGHYSPVFYG
                     TDLAYLYLHQPDVIEQIFAFDHLTILDHSVTVNNVPDGDSYRCKFHSKDGGCLLEQTW
                     RESICRHFVCPGIGWGEESALQEWKDYMEQLTDYEIHLNQRLADALDQQGLCLRNPQS
                     RSDYLRALVQQYQQELEETPAFMCSMPVQQSFKIVRQLHFGKEWPL"
     gene            27682..28311
                     /gene="nadD"
                     /locus_tag="P5549_03540"
     CDS             27682..28311
                     /gene="nadD"
                     /locus_tag="P5549_03540"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRW12179.1"
                     /translation="MAGTQSLGIFGGTFDPVHYGHLLAAQWAGEAFGLDEIIFIPAAR
                     PPHKDPAGVINGRHRLRMVELAIKNNPTFSVSALELERSGYSYTVDTIRHFLLADPRL
                     DIRFILGVDALQLIHTWKEVEQLITLCRFIVVTRPGYELDRADPVFATVPAELWDRID
                     LLAIPGLEISSSEIRKRIAAGRSVRYLLPPEVAEYIKKNELYRNETNDQ"
     gene            28301..28894
                     /gene="yqeK"
                     /locus_tag="P5549_03545"
     CDS             28301..28894
                     /gene="yqeK"
                     /locus_tag="P5549_03545"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRW12180.1"
                     /translation="MINRNEHEILAILERRLSEHRFQHSVAVAQAARELAAIFGEEED
                     LAYKTGLLHDYAKGIPAPQLIAIASENGLIEDEIENKVPDLLHAPVGAYLLKLELGIE
                     NPTVLEAVRVHTMGSLHMSRLDKIIFLADMIEPGRDYPGQDRLDCLARRDLDRAMLYG
                     LDATIRYCLDQARILHPRTITVRNHFLEILRQPIKDE"
     gene            28930..29289
                     /gene="rsfS"
                     /locus_tag="P5549_03550"
     CDS             28930..29289
                     /gene="rsfS"
                     /locus_tag="P5549_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRW12181.1"
                     /translation="MEKEERMARVIAEAALEENALDMVILNVHELTIIADYFIICNGR
                     STVQIRSIAENVEKVMQEKEGLLPLRRDGLEQGKWVILDYGSVIVHIFRQEERDYYKL
                     EKLWGDAREISVNQEMQ"
     gene            29621..>32826
                     /gene="gltB"
                     /locus_tag="P5549_03555"
     CDS             29621..>32826
                     /gene="gltB"
                     /locus_tag="P5549_03555"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRW12182.1"
                     /translation="MKNYGLPGKQGLYDPAFEHDACGMGFVAHIKGEKSHQIIEDALT
                     VLENLSHRGASGADENTGDGAGIMLQIPHVFFQRECSVLGFDLPATGGYGVGMIFAHR
                     YEDFRNTQMAAFAQIVKEEGQKILGWREVPIDKSGIGAGAQSVMPRFIQVFIARDEGL
                     CDDMDFERKLYVIRKRAEKIIIPLCEDKGGSFYIASLSSRTVVYKGMLTAEQLRSFYL
                     DLSDLDFASALAMVHSRFSTNTFPSWERAHPNRYIVHNGEINTVRGNVNWMKARQRVI
                     DSPLFADITKVYPIIDESGSDSAMFDNCLEFLYLTGRSLPHGVMMMIPEPWEANHLIA
                     AERKEFYKFNNFLMEPWDGPAAIGFSDGTVIGAVLDRNGLRPARYYVTKDDRVILASE
                     VGVIDINQANVACKGRLEPGKMLLIDTEARRIISDDEIKSEVARLHPYQEWNGQHIVD
                     LDDMPAAPAAEETDKTYLIEQQKAFGYTFEDIYQTIQPMAAEGHDPVGSMGMDSPLAV
                     LSEQPQLLYLYFKQLFAQVTNPPIDGIREEIVTSSSLPLGSTGNLLDPNHSQAACIYL
                     EHPILTNEQLHNLKYLHDDRFKPAVISILYKASEGARAMNRALERIFRQADQAIADGA
                     NIVILSDRGVNETLAPIPALLASSGLHHHLTRKEIRTKVGIVLESGEPREVHHFCTLV
                     GYGVTAINPWLTFDTVRQLGEQEALGGLSLAEAQKNYIQASVKGMMKVISKMGISTMR
                     GYHGAQVFEAVGLKRELVDKYFTMTPSRLEGIGLEEIAQESRMRHDSAFNRNDPGRET
                     LETGGYFQCKENGEVHLYNPQTIYLLQRACREGDYHLFKEFSRRINEEKVYTLRNLLE
                     FNIIPGDTIPIEEVEPVESIVRRFKTGAMSYGSISQEAHECLAIAMNRLGGKSNSGEG
                     GEDADRYIPDKHGDSRSSAIKQVASGRFGVTSNYLVHAAEIQIKMAQGAKPGEGGQLP
                     GEKVYPAIAKVRHSTPGVDLISPPPHHDIYSIEDLAELIHDLKNANRDAGISVKLVSE
                     VGVGTIAAGVAKGKADVILVSGYDGGTGAAPRTSIRNAG"
BASE COUNT         8499 a         7496 c         8606 g         8125 t
ORIGIN      
        1 gggagacctt ggcggccaat aaactggttt ataccttgag cctgagctac ctggagggca
       61 acgatcagga aaagatcgtt gcccaattgc tgaccttgct gcagccctat tatccggaag
      121 tgacagcagc gcagatcaaa gaaaaaatcg agctgcaaaa atatcgttta tacgagccgg
      181 tcgcgatcat gcgtgatatc ccttgggaac tggtggtaaa gcttgaggaa agccgccagg
      241 atttgcccgg cgttgcggtc aatatagaac cgctgcgggt ctatccccag cagcatctgg
      301 cgggtcatgt gctaggctat atccattcca tcaatgaaga ggaattggca gccagcgggg
      361 aaaaatacag catcaacagc ctgattggca aatccggcgt ggaaaaagaa tatgaaagcc
      421 agctgaaagg caaagacggc gctcggcggg tggaggttga tgcccagggc agaccgatgg
      481 gggaactggt gaccatgtcg tcggtgccgg gcaagaattt gactttgacc ctggatctga
      541 agctccagaa ggtcatggaa tccagtatgg aaagtaacct gcagcgaatc cagcagaccc
      601 atcccaaggc caaggttggt tcagccgtcc ttttagaggt gaaaaccggc gaggtattgg
      661 ccatggccag cagtccgtac atgcgcccgg atgatttcaa gggaaaactg gcttcttctg
      721 tggcggccta ttattttccg gctggagacc aatatgatcc aatggaaccg ggggcggcca
      781 ttaatcgagc tattcaggcg caatatccac cgggttcgac ttttaaaccc atcaccggta
      841 tggcagccct ggaatcaggc aatctggatc ccaacgatta tattaattgc ccggggcgtt
      901 actggattgc cccttatatt acctgtacgg gcgtacatgg caatgaaaac tatttcagcg
      961 gcatggcgga ttcctgcaat acttattttc aggaagcagg gcgcagggct ggcaaggata
     1021 aaataatcga agttgccaag gatttcggtc tggggcaaaa atccggcatc gatctcagcg
     1081 gagaagccag cggactggtg cccacgccgg cctggaaaaa agaaatcaat gccattctga
     1141 ttgatcgcaa atatgatcat ctgcgccaac aactgaccga aaaatatgac cgcctgttgg
     1201 ccgctgcgca aagtgatgaa gaaaaggaag aactgcagca gcaacaaaaa aatgaaagtg
     1261 ccaaactgga agcccagtac aagatagatt ataattttga tacccaatgg caggcctttg
     1321 atacctttaa catgtccatc ggccagggtt ccaatcagta taccgtgctc caactggcca
     1381 attatgttgc tgccatcgcc aacggcggct atctgatgaa accgcatgtc gtcaaagaaa
     1441 tcaccgatcc caacagcgaa caggtcgagc tggtcaagcc gaacatcatt cacaaaacct
     1501 cggtttcgtc cagcacgctg gccttgacca gacaggccat gcaggcggta accggagctg
     1561 gaggtacggg tagtttcctt ttcaccaatt ttcccgccac cctcccggta gcagctaaaa
     1621 ccggtactgc ccagaccggc cgggtagggg acaagacttt gagcgaattt cacggcgtgt
     1681 ttgttgccta tgctccggcc aacgatcctc aggttgcttt tgccggagtg atcgaatacg
     1741 gtcagcacgg cagcgaatca gccggatggg tagccaaagc tgtatttgaa cagtatttcg
     1801 gtctggttga tcattatcag gaaattattg atgccgagga gaaaacccgc agtgcgcagg
     1861 caaaaaaaga tggtgatgcc ataacaagac ccgccggcaa tactacgtcc caagaataaa
     1921 aaaacaataa taagtcgagg ctgatggcga ataatcaaat aattttctgg gggcagaacg
     1981 acttcaaaag aggaaatgcg aggacatttc ctcttttatt atatttttca atagaggtaa
     2041 aatatggatt taaaagaata aactataaat gatgatataa gaatgggcgg ataaggaggc
     2101 ttgctaatgt ctgtggaggt aacgccaaag ggtgcaggct cggacctcat tatcaatgtt
     2161 aaagcttggg gaagctttgc agatatgaaa gctgaattgg aaagaaatct gaatgcgatc
     2221 gacccatatt tagcgggcag tctggtgagc atcgatgtag gtaacaaaag catcaccgaa
     2281 aggcaacttc gggaaatgga ggatatcttg ctggaacatg gctttcatat gaaagaactg
     2341 atcaacgaaa aagaccccag gtctgatcct gaccgccaat gtttttatca aggttttaac
     2401 gaaggtgaaa ataccgtcag caacaatacc accctgattt atcgcagcct tcgttcagga
     2461 caaaaatacc gaagtgaagg gaatatggtc attctgggag atatcaatcc cggagcggaa
     2521 gtcattgccg ggggtaatat tctggtcatg ggtgcactga agggaatcgc ccatgctggt
     2581 gccaatggag atgaaaatgc ggtcatcgca gcctttcgtc tgaatcctat ccaattgcgg
     2641 attgccaacc atatcacccg tccacccgat ggagaatgca ttattgtcaa cacgccggaa
     2701 ctggcaaaga tccgggcagg caaggtcgtt atcgaaaagc taaaaatatg attgggggct
     2761 aaaaaaatga aaagtaaggt actggtgatt acttccggta aggggggagt ggggaaaacc
     2821 actacgactg ccaatcttgg aacagctctg gccatgctgg gcaaaaaagt ggtcatgctc
     2881 gacaccgata tcggcctgcg caatctggat gtggtcatgg gtctggagaa ccgaatcgtt
     2941 tttgatattg tagatgtggt caacggcaac tgcaaaatca aacaggcgct cataaaagac
     3001 aaaagatttg aaggcctgtg tcttttgccg gcagcgcaga ccaaggacaa gactgcggtc
     3061 acaccgcatc agatgaagaa ccttaccaat gagctccgca aagaattcga ctttatcctg
     3121 gtagattgcc cggctggcat cgaacaggga tttaagaatg ccatttccgg cgctgatgat
     3181 gccgtagtag tggcaacacc tgaaatatct tcagttcgtg acgctgatcg gatcattggt
     3241 ttgctggagg ctgcaggctt aagcaactcg aagctgatca tcaaccgcat gcgcagtaaa
     3301 atggtgaaac gcggagatat gatggatatt gatgatatcc tcgatatcct ttccatcaat
     3361 ttgctgggtg tggtaccgga agatgaagcc atcgttgtat ccaccaaccg cggtgagccg
     3421 gccgtattgg agaccacttc ccgggccggg gaggcatatc gccgcattgc cagacggata
     3481 accggggaag atctgcccat cgattcgcag gaggattcca ttaatttggt ggataggttt
     3541 aaaagaatac ttaagttagc caggtagggg gggattcatt ttgttagaac tgataagcag
     3601 aatttttggg aaagaaacaa gctccagcaa agatattgcc cgtgaaagac tgcgtctggt
     3661 actggtgcat gatcgctcga cagtatcacc ggagatcatt aatctgcttc gtgatgacct
     3721 gatccaggta atccagaaat acatggatat tgatatggaa ggattactgg tcaatattga
     3781 aggggaaagc aattctgttg ccctgatcgc caatattccc atcaaaggat tgaaaagggc
     3841 tccagccata taagccaacc ggtgcccgac cttgtttttc gtatatacat acgacccttt
     3901 atcccgctta aagagattaa atatttttta agcgggatgt tgttttctgc tgataaagta
     3961 tgaatcggat gagcagatca caaaaaaatc tgcctgatca ggggctatta tcgccttaac
     4021 cataatttaa catctaagca aatgattagc cgcaccaggg aactggacag ggttgattta
     4081 ttacctctat aatggagcag ggaggggtct gaaatgcaaa taaaacgaat aaagttttta
     4141 gataaaggtt ttctcttcag cttggccata atcctggctt tcggtttggt catccttttc
     4201 agcgccagca gcgggatcac cagcgatcct ttttattatg tcaagaagca gatcttatgg
     4261 ttgattgtag gtctggttat ggccatcatg ctgatccgtt ttgattattc ccagttggaa
     4321 ggatactgga aatatctata tggtatatcg attattacgc ttgttatagt attgattttt
     4381 gggcaggaag tacgcggcac gaccggatgg ataggcttcg gaccattccc caaaatccaa
     4441 ccggctgaat ttaccaaaat cctgctgatc ctggcatttg ctgcctatct aaacaaacgt
     4501 aaagatagtc tggatacttt tcgggaaatg ctgccttgtt tcatatttat gggcattccc
     4561 ttcctgctga tcatgatgca gcctgatttg ggcacagcgc tggtttatat tgcggtcacc
     4621 ctggtgatga tgtttattgc cggggctaac ccaaaagtac tgggaatgat cattttaatt
     4681 gcttcacttt tcattgctct aatgttgttc atgcatttca agttcgatac ctggatgccg
     4741 ctggacgatt atcagatcaa acggctgatc gtctttgttg atccgtataa tgacggccag
     4801 ggcggacggg atgccggctg gaatacgatc cagtccctgg tggcgatcgg ttcgggggga
     4861 ttgaccggca aaggcctgtt taacggcagt caggtgcaat taaatttcct gccggagcat
     4921 catactgatt ttatttatgc agtaattggc gaggaaatgg gatttattgg tgcggtattg
     4981 gttatccttc tttatggaat actattgatt agggcagtca tcattagttt taatgccaat
     5041 gatctgtacg gcagcttcct ggtcatcggc ataactgcca tgtggatgtt tcatatattc
     5101 gaaaatatag gcatgtcaat cggtatcatg cccattaccg gcataccttt gcctttccta
     5161 agctacggcg gcagtgccat gcttaccaac atgctggcgg tgggctttat tctgagtgta
     5221 aacatgagag ggaaaaaact ggtcttttga tcagcggaga ggagcgaggg gatgaaaaag
     5281 gaaggaattg atctggacat ggcgcgagga aaattacagg aagcgattat gctgataccc
     5341 aattatttaa aactgcttta ccggctgacc cgcgacaatc gggttcaggt gacggaaaag
     5401 gccttgctgt tggggactgc cgcctatgtt ctgtcgccca tcgattttct gccggatttt
     5461 attccttttg taggtcaggt cgacgatgtt ttgctggtgg ctttgatcct gaaaagattt
     5521 atgaacagtg tgagccccca ggtgctttat cagcattggg atgggcggcc cgatctgctg
     5581 cgcaatatcg aacagatcct gtcctggtcg cgcttctttt taccggccga tgtatatcaa
     5641 aaagtggtac gcaaatcacg ggaaaatcct ggccatggca gccatgatac gattgacgcg
     5701 gattatgaaa taaaataagc aatttatctt aataaacgta tttttacgaa gcaggctgca
     5761 aagctgacat aaaataacca agcttactat atatttatta aaaagtcagg tggtgagctt
     5821 ggtgttttat agttttaaga ttcgtttgtt gatggtaatc gtcctggcgg ctgtgctggg
     5881 actggttatg caatctgaca gccccggcaa acaggcagtg gagcctgtcc tgcgatacat
     5941 aatgacgtcc gatgacgccc ttacccaaac cttcaatcag cttatctacc acccggagcg
     6001 cagcggcctg gaaagtttgc cggcttcggt ccggaccctg atcaagtcac cctgtgaata
     6061 tatagcggtg gaacagaaat ttggttctta ctggaatgaa gcgcagggtg gtgaaacatt
     6121 caacccggga atgaacttta aagtagctgc aagtacaccg gtcaaggcgg ttttgccggg
     6181 gaccgtacag gcaatcacca aagaaagtcg ggagtatacg gtcaaaatca agcatatcgg
     6241 cggtttggag tccatttatg ggggactgcg ggaagtagaa gtaaaagttg accatccggt
     6301 actgatcgag gaggtaatcg gcaaaaccgg tgagcagttc tattttgaaa tgcgcagcaa
     6361 aaacgatccc gtcgaccctc gcagtatttt tgaataaaaa tgattctcgg taaaattgcc
     6421 gggataaggg tgcggatcaa ctggcttttt ttactggtct gcgcagttta tacctggctg
     6481 gggctgggca gcgaaatact cataattttt gcttcggtat tgctgcatga actcagccat
     6541 accatcatgg ccaaaatatt aagggtgcaa gtcgcggaag ttgaactgct gccttttggc
     6601 ggtcaggcca aaattgaaga tttcaccgga cttgatcccg accgggagat ttatatcgcc
     6661 ttggccgggc ccatcatgag cctgtccctg gcggcttttt tttatttgct gccgccccac
     6721 cttttgcata aaacccctct attgcttcag atcaacctgt ttttaggcct gtttaattta
     6781 ttaccggcgc tgccgctgga tggcggccgg atcttgcgag ccatcctttc cctgcggatc
     6841 ggatataaga gagctacttc cgtagcagcc ggtttgggaa aaaccatcgc catccttctc
     6901 tgtgcctatg gcggctacct tttttatctg caacaaagcg gtgccaacca tatcctggcc
     6961 ggtttaatat tattttgggc ggcccggcgg gaaggattgc tgctgggtta cgcctttatg
     7021 cgctatttgg ttaataaaaa aagtgaactg gccaataaag gctttttggc ctctcatcag
     7081 gtggtgagtt ttgaagatac cctgatcaag agtattcttg cctcgacccg ccccggctat
     7141 tataccatgg tcatggtgct cgaccagcaa catatgccgc aaggtttgat cagcgaggcc
     7201 caaatgattg aaagcctctt gaaccatggg ccacgggtgc ggctaaaaga ttgttgaccc
     7261 taatcgccag aatgatcagt ccaaacctat cctgccaaaa ttgtacactc aaaagattgt
     7321 taagcctaat ccaaccggat gcgggtaaag gccttgcatt gcagcatggc tttttccagc
     7381 tcttcataat cacatttgct gcaagcggca catctttcca ctgccagatt gttatggaag
     7441 tgtttgcgca ccgccagcgc caacactctt aaatcctcca gctcctcggc ctgggcttga
     7501 tttgatttga taacctgcgc ttcccgtgcc ttaagagcag cgatctcatt atttttggct
     7561 gccagttccg cttccaactg ctttataaat tgttctgtat ctttgctggg caattttttc
     7621 tcctccttgt tgctattcga gcttcaactg agccccattt ttctcctttc aacaggataa
     7681 cggctgttgc cttttttggc aagcgtagcc gttatcgcgt tgctattggt tttataattc
     7741 cagtttgcgg tcgtagatca ctgctgccag gatgaataat atgatcgcca gcagcagata
     7801 gcctagactg gaaagataca gactgttgac cagactatct gtattgcccg acatccgtac
     7861 catgaccagg ttaacctcat ggctgccggt tcccagcgct tccagtaatt taccggataa
     7921 gatcagggtg aacacaaacg ttactgccgt gaccaggccc gagaattttg cgcttaacct
     7981 gccggcgatc tgactgaaga agctggtgca gcatagaaaa accaggaagg ccagtccggc
     8041 cagatagaaa gccaataaat tgctgactaa ttccggatta ttggcaagca ccgccagatc
     8101 cggctgatag ggtgcgatgt attgaaaaag tttggtggca aaaagcagat atccgctcag
     8161 accgagcaaa agcgttcccc cgacatactg ggtcagcagc accatcattt tggaagctaa
     8221 aatcatggcg ccggataccg gcagagacat gaccagatac accgtgttat ggctccattc
     8281 ccgcgacaac agcttaaacg aagatagcag cggcaagaat ccgaccaggc ccaacagcaa
     8341 aaacaagggg aataaaaatg ccggaggagg cacttctgaa tccaggctga aaccaatggt
     8401 caccagaacg actccgatta ccacgatcaa aatctctgct gtgacttcgt gcaggtcttt
     8461 caaatacagt ttggtgaaca ttttaagttt actgttggac atggcgaaag gcctccttca
     8521 ttatttcaac cagcgacaac ccctgggtta ttcgtaattc ttccgccgaa ccgctcacta
     8581 cgatcctgcc ctggtcgata aagaaaacct cgtcgaccag gctttccaca tcatttattt
     8641 catgggtgga aatgataatg gattgttctc cttcccggaa atcgcggatc aaggtgttga
     8701 taatttcttc ccgggtcaaa atatcgatgc cggaaagcgg ttcatccagc agtagatagg
     8761 gagcgcgccg ggagacggtt aaaatcaatt tgcatttggc ccgttgtccc ttggatattt
     8821 tactgatttt catatcctcg cgcagattta aaaagccaag cagttctgtg tatttacggt
     8881 cgtcccagtc agcataaaaa ctgcgcataa aatctgccgc ctgctttaac gtccaccatc
     8941 cgtacagatg atcgatctcc ggcagaaagg cgattttgac ccggctgtgg cggggctttt
     9001 gaccgtccac cagtacgtgg ccgctatcgg gctgatttaa accagcgatc atcttcatgg
     9061 cagttgattt gccggctcca tttggtccaa acaaaccgat aattttgccc gggccgaatt
     9121 gtaaactcag ctgatcaagc gcctgtatgc tgccgaaacg cttgccaacc tgatcaaatt
     9181 caatcattgg tagttgctcc cttcatctcc attaatcggc ggtttaatac ttcagtgatc
     9241 tgttctttgg catagcccaa tgaattcatc tctgcgaaaa agcgattcag gatgtcgtca
     9301 gccatcgctt gcctgatctc gctgatcaaa gtcgtatccg ccttgacaaa agtaccctgc
     9361 ccgcggatcg tctccgccag accaatcctt tccagctcct gataggctcg ctgcacggta
     9421 ttgggattga cacttacctt cagcgcgtat tcccgctgtg aaggaatctt atctccggga
     9481 cccaactccc cgcgaaccaa ttgcttttga aaatcctcaa tgatttgttt ataaattgct
     9541 tttgatgtat cgtagtccag ttggctcacc tcatccttat gcttagtgca ttaatgtact
     9601 agtacaccat gattttaatt aatgcttttt cttttgtcaa gcagcttgct aatttcttta
     9661 aaggatcact acaataacat tattaagatc tgcaaaagct gataaaaagg aactgacatt
     9721 agaatgaaaa aacatactat caagccgctg cgtcagccgg acagaattat caactactgg
     9781 aaaaatgaca aaccagtggc ggcttttatt atcgtctttg gcctgggcta caatattgcc
     9841 gccatcctgg gccccattta tcagggcaaa ctgattgacg cgctgctgcg cggggacggg
     9901 ctggcggcct tgatccggct ggcggttttc tttgtcctca tgatcgcggg catccagctt
     9961 atgcgctacc tgaaacgctt ttacacccgc cgttttgcca atcagaccgg tgccgtcatg
    10021 cgcctgatga tttacaacag cgtaatcaat aaaagttatc tggagttaca acatgaaaat
    10081 atcggggatc taatgaccag agttctggcg gatgtagatt tatgtgttga aggcatgcgc
    10141 aaatttacga ccgaagtttt tgatactggg gttttgatgg cttcgtatct ggccgccatg
    10201 ctctattacg atgtaaaaat aaccctcctg ggggcgattt ttattcccgt ggccatgatc
    10261 atcgcggaaa aactgaaaag gattatatac aagtattcca ttgcttatag aacaaaaagc
    10321 agtattatca cgggcatgac ttatgaatat attgagcatg ccatgctata ccggatcaac
    10381 ggcctggaga ggcaaagccg ggagaaatac catcaggaac tcaatgatct gcaggaccag
    10441 gcgataaaag ccaacctttt tggcaatgcc atgcagcccg tctataatgt ggtggctatg
    10501 ctgggcgtga tcatcgttat atacctgggc ggaaaccgag taatcgaggg aagttggacc
    10561 gtgggaaact tttctgccta tatgatcatg tttgccgcgc tggccaccaa agccagcaaa
    10621 gccgccaatc ttttcaattc agtacagaag tcacagattt catggcagcg gataaaaccc
    10681 tattttaggg aatatcaaag ccaggatacg gctcaggata tccgccaggg tttaacctct
    10741 ttgaccattg aggatatgag ctttcactat cctgaagcgc aggaaaatgt tattaaaagt
    10801 atcagcctca atgcccggca gggagagatc atcggtgtga ccggagcgat cgcctccggc
    10861 aaaaccacgc tgggcatgtc tttgctggga ttataccctt atgccggcag catttgcatc
    10921 gacggcaagg aactgcgtga ctattcggat tatgagcgca gtcagatgat cgcctatctg
    10981 ggccacaatc cgcagctgct gtcagatacg atttacaaca acatcacttt gggcggtaat
    11041 gaagacataa gcgaggtttt gcatgatgtt tgtctggatg aagacctgcc ggccatgcca
    11101 caaggggcag acactatggt cggcagcagc ggcatccgcc tgagcggcgg gcagcaggct
    11161 cgtattgctt tggccagaac tcttttgcat aaagccaaaa taattattct tgacgatcct
    11221 ttttcggcgg ttgatatggg cacggaagaa aaaatcatcc gcagtctgaa aaacaactat
    11281 cagcagtgct tgatcatcct gatatctcac cgcctggcca ttttcagcca gattgatcgc
    11341 atcgtgctgc tgcatcctga tcatagcttt gattacggaa ctcatcatca gttgatggaa
    11401 acatcagagc tgtatgccag catctatgaa ctgcagcgat tggcaggtga gcaaaatgaa
    11461 taaaagtgta gtcaaaaaat cgttctgttt tactgccaaa aacaacaaga tgatcatggt
    11521 gctgctgatc ctggtcgtgc tgggggcggt gactgccagc ctgatccctc cgcagatcct
    11581 tagactggtc atcgatcaaa acctggtgcc taaaaacccc aacggattaa tacgcctggc
    11641 ggctttctat ctggcggtat tggtactggt cggcatcttt gatttcataa aagaattcat
    11701 tttgacggtt atggggcaga aggtcagcaa agagattcgt ctggccatga tggctaagct
    11761 tgcacgtgta aacgccggat atttttcctc ccaggattcc ggagcactgg tttcaaggtt
    11821 caccaacgat gtcgatgcga tcaacaccct gtttgccagc ggggtggcag gcatgttggt
    11881 gagcgttttc aagatcgtgg ggatcgtggt atcgatctgg ttgttcagtt ataaactggg
    11941 gttgttcaca ttattgtttc tgccggtcat atattttata acccgcttgt ttcagaaacg
    12001 tatgctgatt gcgcagatca aaaaccgtgt cctggtcggc aaggtcaaca accatatagc
    12061 agaaagtctc aataatgtgc tgatgattaa agccggcaac aaggaagagt atatggaaca
    12121 gaattatacc ggatatttgc gggaaaattt tcaaaccctt gagaaagtga atttctatga
    12181 tgctgtattc tctcccctta ttcaggtcct gcgggcattt gtaatagcct ctattgttat
    12241 tatgtctgcc aatcagctcg gctggctggg gatttctctg gggatggtgg cggcctccat
    12301 cgaacttatc tccaatctgt ttgaaccggt ggagaatttc ggcatggaac tgcagaacat
    12361 tcagcaggcc gtttccggta ttcgccgggt tgatgagttt tatggcgaac ctgaggaaac
    12421 aggaaagaat gaggagttga cagcagagac cgtgattccc gatcgtgcct gtgtcaggct
    12481 cgcttttcgc aatgtgaatt ttgcctatga agcaggcaat gaagttttac agaatatcga
    12541 tctgtccatc aaatcccggc aaaatgtcac ttttgccggc cgcaccgggg tgggcaagtc
    12601 cacactattc aaactggttc tgggtttgct gcagccccag gaaggagtta taacgatcaa
    12661 tgacatcgat gtatgcagca ttccccatca tgagaaacgc aaaatctttg gttatgtcga
    12721 gcagggtttt catctgattg acggcagtgt agcggatcag atcagtctgc aggatgccca
    12781 tatatctcgc actcaaatcg agcaggcgct ggagttggtg ggtatgaggg agtatgttga
    12841 acagctggaa aatggcctgg atactgaggt gaaaggtgat acgcagttct ctcagggaca
    12901 aaagcagctg ctggcaattg cccgggccat cgtcaccgac ccgcccattc tgctgctgga
    12961 tgagattact gcccatctcg attccctcac cgaagagagg atcgttgcgg taatgcgcaa
    13021 agccggccag gcccggacca ttttatcgat ctcccatcgc ttgtcatcgc tggttgccag
    13081 tgatatcgtc gtcattttgg aaaacggccg catcaagaat gctggctcgc cggagatgct
    13141 cctgcaagat gatgattggt atcgcagtca tattgctctg gaaaaactaa cttggaaata
    13201 attcacacta ctttatggcc aaactgggat attcctgtta tactgcaggt aaactatgct
    13261 aatcagagac ccttcgctat cgctcaggat gacattgctg cgtaattttt gaatgatgca
    13321 aatccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttc gctatcgctc
    13441 aggatgacat tgctgcgtaa tttttgaatg atgcaaatcc tcaatcagca gaacggaaag
    13501 gaatttgcca tgatcgacaa tctgaacaag gccccgcgca atcaaaccgt aaaattgatc
    13561 atcctggccg gcatggtgtt gctgctggtt atgtatttcc ctgcggtcat caacaagacc
    13621 aaagccctcc tcgcagtttt ctatccgtta ctgctggggg ctttcattgc tttcattctg
    13681 aatatactga tggccggata tgagggaatt tattttccgg ccagccggaa ccgttttatc
    13741 aacgcgaccc gccgcggcat gtgcctgatt ctggcgatct tgactattgt gctgatcatt
    13801 tattttgtgc tgcatatcgt tattccccag gtggggcata ccattgcttt aatcgcggcc
    13861 ggttttccgg aagcctataa tgacgcgctg gtttggctcc agcaatattc tgacgaaatc
    13921 ccgggtttgc aacagagact ggcggcactc gacatggatg gctccgccat tttgcagcag
    13981 gccctggctt tgttgggggg ctgggccttt ggtacggttt ccatgatcgg ctcggtattc
    14041 ggcaagatgg ttaattttgt cttggctctg gttttctgca tatatatttt gttggacaag
    14101 gaaaacctca aagtcaaatt tgacaaattg tttcgggcct atctgcgtta tgaccgccgg
    14161 gagaaacttt atgcggattt gcgcgtaacc aaccggacat tttctgacta tattgtcggg
    14221 cagtgcaaag aagcggtcat cctgggcgtc ttgtgtgcct tggggatgtg gatttttggt
    14281 ttcccctatg ccttgtcggt gggatcggtc ataggtctga cggcattgat tccaatgatt
    14341 ggggcgtata tcggtgcagc cgtcgggttt ttactgattg tcatcgtcga tccgcttaag
    14401 gctttgctgt tcatcttgtt tattgtcgtc ctgcagcaac tggaaggcaa tatcatctac
    14461 cccaaagtag tcggcaacag cattggcctg cccggaattt gggtcctggc agccatcacc
    14521 gtgggcggtg gcttgatggg aataaccgga gttttgctgg gcgtaccggt tgctgcaacc
    14581 atttacaagc tgctggggca agcggttaat gccagactcg ataaaaacgc tccgattgcc
    14641 gatgagagta tgtagatttg ttattctaac cgttggtcgg ctggttttgc gaaggtgagt
    14701 taatgattca gggggttgtc gttcgaatgt gttaaaatgt atgctaaggt gagtgttagg
    14761 acgaatgata cgagtcgttt atgaaagaga ttgaaggaca ttgatcctgg catagccggg
    14821 tatgagggat cagaggaaat ataacaaaaa ccaagaaatc catcgacatt tcgcggccgg
    14881 tggattttgt gctaatatag taaaacaatg attttaacca acggaggcca gataatgaaa
    14941 gagaggcttt tcagggatat tttgccgcgg gtggagaaac ccgctcgtta taccggcagc
    15001 gaagtgaata tgatcaagaa ggattgggac agcaaatcag ccaaaatggt gatggctttc
    15061 ccggatgtgt atgagatcgg catgtcccat atcggctgca aaattctgta cgggttggtc
    15121 aatgagacca ccaatcattt gatggaaaga agctttgcac cctggccgga tatggaggcg
    15181 ctgctgcggc aagaggaaat ccccttgtat tccctggaat cgttcactcc gctggccaag
    15241 tttgatgtgg tcggcttctc tctgcagtat gaactgtcca ttaccaatgt gctaaacatg
    15301 ctggaactgg gcgggatacc gctgtgcagc gcccaacgca gcgataccga tccgttggtc
    15361 attgcaggtg gaccggtggc atttaatccg gagccttttg ccgacttctt tgatgctttt
    15421 ctgttgggcg acggggaaga ggtgaccgtg gaatttcttg attgtgtgga gaccaaccgc
    15481 catctggaaa aagctgcgtt gctgcaggaa ctcgctcaga tcgaaggcgt ctacatcccg
    15541 gccctgtatg aggtatctta tcatgcagac ggaacgataa aatccttcgc gcccagcgct
    15601 gaagggattc cgttacgggt tcgcaaacgg ctggtccggg accttgacct tgcctattac
    15661 ccgtcgcagc ccgtcgtacc ctatctgggc atcgtccatg accgggcggt gctggaagtc
    15721 atgcggggat gccagcgggc ttgccgcttc tgccatgccg ggatggtcta ccgtccggtg
    15781 cgggagaggt ctgttgaggt attggcacgg caggcggagg aacaactgaa gaacactggc
    15841 tatgatgaaa tctcgctggc gtcgctcagt accctggact actccggggt ggagatcttg
    15901 gtcaaacaac tggtggagga atatggccgt caagggatcg gtgtctcgct gccctcgctg
    15961 cgggtggatg cattttccat cgagctggcc aatgaggtgc aaaaggttcg caagacgacg
    16021 ctgacactgg cacctgaagc tggcacccag cgcttgcggg atgtgattaa caagaacgtt
    16081 accgaggaag atttgctggt ggctgcagaa gctgttttca aatcgggttg gatgggatta
    16141 aaactttatt ttatgattgg cttgccgacg gaaagcgaag aagatctgca aggcatcctg
    16201 gaactgttga aaaaagtcaa gtcgatcggc agccgttatt cccgccgggc gattgaaatc
    16261 agggcgagtc tggcctgttt tgttcccaaa gcccataccc cctttcaatg gcaaccccag
    16321 gccggcatca gcagtctgga ggaaaaacgc catttcatgt tgaaacaaaa aatgaagaat
    16381 gtaaaactca gttttcatga cagccgtacc agttatctcg aaggggttat ggcccggggg
    16441 gacagaaaac taggtgaggt gattcaccgg gcctggcgca tgggctgcaa atttgacggc
    16501 tggtcggagt atttcagttt taccacttgg agggaggctt ttgcggaatg tgggatcgat
    16561 cccgattttt atgtccgtga acgaagccgt gacgagattt tcccctggga ttttattgac
    16621 attggtgtgg acaaggacta tttatgggaa gagaatgaac gtgcattcca gcgcatcctg
    16681 accccggatt gccgccagga cggctgtgtc agctgcggcg tctgtcccca gttcgagttt
    16741 gagccgttca gcggggaggg ggcgtaaaat gcgtttgcgg gttgagtata gcgtaggtcc
    16801 ggaactgaag tttctggcca atctggatat gatgcacctg atggaaagag ctttgcgtcg
    16861 ggcaggcatc ccctactatc ttagcgaagg cttcaatccc catatccgac tgtcgatggg
    16921 gacggtactt ccggttggga tttggggcaa gcacgagtat tttgatctgg atttggcgga
    16981 gatggacagg gaggatttca tatacaacat gaacctggca ttgccgccag ctttatcaat
    17041 catggattgt cagcagatcg aggaaaacgc tccctcgctg atgaagatca ttaatgccgc
    17101 cgaatatact ttcgtgattg aggcgggact tgatttgtct gacttaacga ccagaatcat
    17161 gcagcaagat acgctgccgg tacaaagtcg gggaaaaaac aaaaacgtga tcaaggatct
    17221 gcgacccggg atttatcaga tacggattga ccgggatgag gcgtatgatc tgctgtcgct
    17281 catggtcagc atcaacgaac cattgaatat cagatatgat gaattacagg cattattcat
    17341 cgaatacgga ctgtttgcca aaaatattgc tgacattttc cgccagggga attatattaa
    17401 agaagaccag cagttttatt caccattaaa gagggtgtaa tcattggtaa gagatattat
    17461 tggtgagatc tatccctggg aatctcgcgt ggccattgtg gaagatgacc gtttggtaga
    17521 ggtcttttgg gctgatcaaa atgaaagcgt gggcaacatt tacaaaggca aggtgaaaga
    17581 tgtattgccg ggcttgtcct gtgcattcat cgatattggg ctcagcaaga atgctttttt
    17641 atatgtggcg gatgtgataa ctgcaggcag ccgcaaagac aaaaacattg gccagctgct
    17701 gaaaagcggg caggagatca tggtacaggt gaaaaaagag gctttttccg aaaaaggtgc
    17761 ccgtgtcacc tgcgacataa ccattcccgg gcatctgctg gtgctgttgc cctttcagcg
    17821 tgaagtttcc atctcgagaa aaattatcgg caatcaaaag cgcaacctgc tgcgcagttt
    17881 ggtggaagaa aacaaacccg aggatatcgg ggttatttta cgaactgctt gtatggaggc
    17941 ccgtgatagt gaaattctgg cagaattgaa tcatttgctg ggaacctggc aggatatttt
    18001 gaaacgcttt aatcagcaga atgctcccag tctagtttat gaagatatcg atgtgatcga
    18061 gcgagctttg cgggattatt tggacgctga tatccgtcgg gtggtcacca acaatttaaa
    18121 aatcaaggaa aaaattgacc tgatcgcttt aaaaaagaac acttcctttg tgcccagtgt
    18181 tcattttgaa gaaggcgact tgttcgaaaa atatggttta gaaaaggata tccgtaaagc
    18241 cctgcggcgc aaggtatggc ttaaaagcgg cggttatctt atttttgacg tgaccgaagc
    18301 gatgaccgtc atcgatgtca acagcggcaa gtttaccggc aaagacaatt ttgaagaaac
    18361 agtttacaag ctaaatttgg aagcggcttt ggaaattcca aggcagctgc gtctgcgcag
    18421 tattggaggt attgtcctga ttgattttat cgatatgcag gacaagaata acgaagacaa
    18481 tgtgatctcc atcctgcgcc aggaactgga aaaagacaag gcccacaacc ggattatggg
    18541 catgacccaa ctgggcttcc tggagatgac ccggaaaaaa tcccgctacg gcatttccga
    18601 gatctttacc gatgaatgca tcacctgcca cggccgagga cggaccatca atctctttgc
    18661 cctggcctgc gaagcaaaac gcaaactggc caatatgggt tatctggaaa gtgaagagat
    18721 cgtttgcgaa gcgcatcccc aactgctgca atacctgata aatgaagaga acaacatgaa
    18781 ttatatccgc aaaaaaaccg gcaagaaaat ccgtttggaa gaaaacagca gcatgaatat
    18841 cgaagagtat aatctgtaca gcacatagct tgacggtggt tttaaattgt gttaggctta
    18901 gctgtgggtt gcacgttttt gcaaccgctc ggtccaggct ccaaaatggc attccctgcc
    18961 tgcctgacga tcggcgagtc tgaaagatgt ggaggtgtat tatgtacgca attattgaaa
    19021 caggtggcaa acagtacaag gtatccgagg gtctaatgat caaggttgag aagcttagtt
    19081 gcgaagcagg ggacaaggta accctggatc aggttttaat gctgagcgat gacaacggaa
    19141 ccaggatcgg caatcctctg gtggagggcg caaaagtcac agcagcggta ctggaacagg
    19201 gacgaggcaa gaaaatcatc gtctacaagt acaagaaacg caagaatgag cgcaagaaac
    19261 agggacaccg gcaggcctat accaaactcc tgatcgagaa gatcgagggt taagcaatag
    19321 aaatccggat tacgaacatt tatttttgaa gatgtaacac tgggaggtgc aatcatggct
    19381 cataaaaaag gagttggcag taccagaaat ggacgcgaca gtgaatccaa acgactgggc
    19441 gtcaaaagat atgatggtca ggtagtgcgt gccggcaaca ttttggttcg tcagagagga
    19501 accaagattc acccgggcaa caacgttatg attggtggag acgatacgct ttttgcagta
    19561 gttgacggca aagtgaaatt cgagcgcaaa gacaaaacca gaaaacaggt cagcgtatat
    19621 ccagtcgacg aaagcgctac ggtttagcac agcaaatttc gtgaaccggg gcgatgccca
    19681 agggaaagaa caaagcatga caaaagtccg caattgagac gtatcttcag tacgttgatc
    19741 gagcggactt tttgattcat ccaggtgaag tgactgccag tttttggcgg gtgcccccgg
    19801 cgcagtctgg agttgtccgg tacagctttc atacatcttt aagatttgaa aggttgctcc
    19861 agcagaaggg gattattaaa agtataaata tgccctaatc atcacattca acccctggag
    19921 tattgcaggg gtatttgtta ttattaggaa taagctggtg tttataccaa aggaacatgt
    19981 gataaatatt tgccgggtat acttaagaat tgtttaaaga agggggaaga acgcgatgga
    20041 agccggacaa attgtgagta tgctgcgtcg ggtacgacac gaatacgcca atcatctgca
    20101 ggtcatcagc ggatatctgg atttaggctg ggaggggcaa ctgggttcct atgtccgggc
    20161 aatcataacc gagctgaacc aggaaagaat tatttttgat tccctgcccc cggaagaagc
    20221 cttgtatttt tatgaacagt tgttaatggt aaaagatctg ggcatcatac tcgtgtatga
    20281 tgatctggat gtgagttcga ccgggtattt aagggaaagc aatgaaccgg tcaacagtat
    20341 tgcggcgctg caatcggata tccaggcgaa cgacgaggat ccggttattt atctttccat
    20401 ccatgaaaat gaaggcggga tagatttgta tttctgctgt gaaagctggg agggagaatc
    20461 ccgtcacatc catattgaca agaggtgaac aaatgtttat tgatcatgct aaaatttatg
    20521 taaaaggcgg tgacgggggc aacggctgcg ttgctttccg acgcgaaaaa tatgtgccca
    20581 tgggcggtcc ttatggcggt gacggcggac gcggcggcaa tgtgatcttc gtagccgagg
    20641 aaggcatgac caccctgatg gacttccgtt atcgcaagca tttcaaggcg gaacgaggca
    20701 atcacgggtt gggcaaaaac cagcatggtg catggggaga agatctgatc atcaaggttc
    20761 ccatgggcac catcatacgc gatgacgaca ccggcgaatt gttggcagat ttgaccgaac
    20821 acggtcagca ggcggtcatt gccaaaggcg gtcggggagg caaaggcaac agtcattttg
    20881 ccaacgctac gcacaaagct ccctccatgg ctgagaaagg tgaaatcgga caggaacgct
    20941 ggatcaatat ggaactgaag ctgctggccg acgtaggtct ggtggggtac cccaatgccg
    21001 gcaaatcaac cttgattgcc caggtatcag cagcccggcc caagattgcc gactatccct
    21061 tcacgacact gattcccaat ctgggggtcg ttggtaccaa tcatcatgaa tctttcgtta
    21121 ttgccgatat ccccggtctg attgaagggg ctcatgaagg agtaggactc ggacatgatt
    21181 tcctgcggca cattgaaagg accagagtcc tgctgtttat tctcgacgct gccgagacgg
    21241 acggacggga tgtacaggaa gatctgcgga tcctgcagca ggaactgcag cttttcaatc
    21301 cggaacttgc caagcgccct tatttaatcg tagccaataa aatggatatt cccggagcag
    21361 atgaacgttt tgcggcatta aagaaagtcc acggagataa agtgctggcc atttccgccg
    21421 ccagtggtca gggggtcagt gaattgaccg aaaaaaccta tgccttgctg cagagtatcc
    21481 cccggcagga agccccacca gaagaggtca gggtcattcg gcgatatgag gatgagccgc
    21541 cctttcaagt cgaagtggtc aatggcatct accaggtaag cggcaagcgg atcgagaatc
    21601 tggtcaacat gaccaatatg gagcaggaag agtcattgca aaaattccag cgcacgatcg
    21661 aacggatggg actggaggac actctgcgcg agatggggat caaagaaggg gatctggtca
    21721 gaatcgctga tctggaattc gaatacagtg aataatcgtt tgtaattaaa aaagaatagc
    21781 gaaatacatg gagtgtacgg acaatgagtg taagaggaga attcaagcag atcaaacggc
    21841 tggtggtgaa agtcggtacc agcacgctga ttcatgccaa tgggcaactg aatctggaac
    21901 gcatggaaag actggttcgg gaactggctg atctgcacaa tcgtgatatt gaagtagtgc
    21961 tggttagctc aggcgcggtc ggcaccggag ccaaccgcat gaacatgcgc aaaattccca
    22021 agacgctgcc ggagaaacag gcgttggcag ccattggtca ggggcgcttg ctgcatatgt
    22081 atgagaagtt ttttgctgat tatggcaaga cagtcgccca ggtgctgcta acccgggagg
    22141 atctggacga aaggatgcgc tatctgaatg ccaccaacac cttgctggcc attttgaaca
    22201 tgcaggtgat cccgattatc aatgagaatg atacggtggt ggtggaagaa atcaagatcg
    22261 gcgacaatga taccctgtcg acgatggtcg ccgggattgt caatgctgat ctattgatca
    22321 tcctgtctga cgtggacggc ttttatgaca atgaccctcg tacccaccga aatgctcgtt
    22381 tgcagagcga gatatacgaa gtcaccgagg aaatggaagc ccatgcggta gcccggggga
    22441 caacctttgc cagcggaggc atgctgacca aattaaaatc cgcccgcaac tgcatggcgg
    22501 ccgggattcc catggtgatt gccaattccg ccgcagagaa tgtcattcga cgcatcgttg
    22561 ccggagagcc actcgggacg gttttcgtgc cgcgggaaga aaaaatgccc tcgcgtaaga
    22621 aatggctggc ttttggctcg gctgaacacg gacaaatcca ggtcgatcac ggtgcagagg
    22681 gggctctcct caatcgcggc aaaagtttgc tggcctccgg agtgacggca gtggaaggtg
    22741 aatttgaacg gggtacggtg atcggggtga taaaccatgc cggcaaggaa atcgcccggg
    22801 gcctgaccaa ttacaacagc attgagatcc gccagatcgc cggtcacaag tcggttgata
    22861 ttgaaaagat tctgggagcc aaggattatg atgaagttat ccacagagac aatctctggg
    22921 ttaaatgttg atagggggag aaatgcccat ggacagggaa ctggaattat tattactgga
    22981 gcaaggccgc agggccaagc aagcttcccg ttttctggcg actgccggga cggaactgaa
    23041 gaacacagct ttgtttaaaa tggcggaaca actggaaaaa cagagtcagg agatcatcgc
    23101 cgccaatcag attgacctgg atcagggaga gaggcagggg ctgaccaaag ccttgctgga
    23161 gcgattgatg ttaaatcaga cccgtattca ggatatgacc cagggactga gggagatcgc
    23221 cgccctgccg gatccagttg gagagatgct ggaaatgcac catcgcccca atggtctcga
    23281 tattggccga atcagaacgc caatcggcgt catcggcatt atttacgaat cacgtcccaa
    23341 tgtgaccgcg gacgctgccg gtctctgcct caagtcaggc aatgcaatca tcctgcgcgg
    23401 cggtgaagaa gccttgtatt ccaaccgggt catcgcccgc atcctggcag atgctgctgc
    23461 tgaatgcggc ttgccggcag gcaccattca gctggtggac agcgaagatc gggaagccgc
    23521 cgttttcctg atgaaaatgc atgattatct ggatgttttg attccccgcg gcggcaaagg
    23581 cctgaaaaag gcggtactgg agaatgcgac ggtacccgtt atcatgaccg ggatgggcaa
    23641 ctgccatgta tatctcgacg catttgcgga ttatgaaaaa gccctgccca tcgtgcacaa
    23701 tgccaaagta cagaggccgt cggtatgcaa cgccgctgag accttgttgg tgcatagccg
    23761 ggtggcggaa gcatttttgc cgggcatcat tgccgatttg cagtcagctg gagtagagat
    23821 tcgcggttgt gcgcagacat gcagtatcgt acccgatttg accgcagcta cagaggatga
    23881 ttgggcaacg gaatatcttg atctgatcct ggccgtcaag gtagttgatt gtctggaaga
    23941 tgccatcgag catatcaacc agtatgggac cggacacagt gaagccatcg tcagcgagca
    24001 ttatccgtca gttcgtaaat ttatggcggg tgttgatgct gcggccgttt atgccaatgc
    24061 ttcgacccgt ttcaccgatg gcagtgtttt cgggttcggg gcggagatgg gaatcagcac
    24121 ccagaagctg catgcccggg gaccgatggg cttacaagaa ctgaccacca ccaaatttgt
    24181 catttatggg gacggacaga tcaggtaatc catggccaag aaaagaagaa atcaagtata
    24241 atagactcag gtcgatccag aatcataaac cagaaaatga aaggagcgtt gtaatgaaga
    24301 gtttaaaagg caccaaaaca gctgagaatc tgctgaaggc gtttgccggt gaatcccagg
    24361 cccgcaaccg ttatacgttt tatgcttcgg ttgcagacaa ggaagggtac aagcaaatca
    24421 agaatatctt tattgaaacg gctgacaatg aaaaagaaca tggcaaacgt ttctataaat
    24481 ttcttttgga gggcttagcg ggagagctgc ctgccttcat cgaaatcaat gccggttacc
    24541 cggtagccca gggtaccacc ctggaaaatc tgcaggctgc tgccgatggt gaatatgaag
    24601 aatggcatga tctgtatccc gaatttgcca aagtagccga ggaggaaggc ttcccccagg
    24661 ttgctgctgc ctttaagatg atcgctctcg ccgaggtccg ccatgacacc agattcaaga
    24721 aactggcagc caatatcgaa aacgataaag tgttcaagaa agaagccaaa accctttgga
    24781 aatgcggcaa ttgcggttat gtccacgaag gcggggaagc gcccaaaatc tgtccagcct
    24841 gtatccatcc gcaggaatat ttcgaggtct ttactgagcc ctattgattt aaactggcgt
    24901 aaaagccatt gttttcttca acgcgatggt tatgatccga gcaacagtga agcccatacc
    24961 cctatgtcgg gtatgggctt tttttaaact gaaggttgat gtttggcaac cttgccggat
    25021 aggtgaaaac gctgacttat ttccctggaa tatgactgcc gtcgcagaaa ggtggatttt
    25081 tggtcttgcc gcagcggcaa agcacaaaac gatctccttc gccgatgatg gtgccgtcaa
    25141 tatcaatgat ccgcactggg gcttcgacca gcagcggacc ttcccgtatc acccttattc
    25201 tgccggcttt ttccggatcg attttataat cctttgaccc cggtcctttg gccaggttgg
    25261 gatcgacgga agatccctcc ggcaggctgt actgcaaaga ccgactggga cacttgtcaa
    25321 tggtcgcgat gattctttcc ggactggtac cggaaatatt gacccaaggc cgtttatcca
    25381 tatcaaacac ttcaggcagt tcttgccagc acttgccggc atgggagcat tcctgcgggt
    25441 accagtaaac gataatgtcg ttgttgcgat attccttgac ggttttttgc ggctttatct
    25501 cagccggcgg cgtgggactg gcttcttgct tggcaaatag ttccttttcg gctccgcaca
    25561 ggggacagac ccaatcggag ggcagatcct caaaagccgt cccggcagga aaaccatttt
    25621 ccggatcgcc ttggactgga tcataaagat atccacacac cgtgcattga taaacagcca
    25681 aattattcat accaacatct ccatttcttg agacatatca ttattttata aacttattca
    25741 atgatttcca agtgatagct ggccagtttt tccaggtcct tctggtccaa tgcataaacc
    25801 cggtccagtt cctgacgcgg ggtttcgcat ccacacaatg aagtggacag gtagcgcaat
    25861 ccattgtcat tgatgatggt gacaatcatt ttttcatcgg ggtatttttc cgccaccata
    25921 cgcgcggcag caagattaca acccgaggaa atgccgcaga agatgccttc cgtacgggcg
    25981 atttcctgtg ccgtggccac ggactgctcc gagctgacca ggataatgcc gtcaatatat
    26041 tgcagatcaa ggatctctgg aataagacca tctgccactc cctgaacttt gtgcttgccg
    26101 atctttccat aagccaaaat tggagactcg gccggttcgg agatatagat tttgatggca
    26161 ggtttttttt ctttcagata tttggcggta ccggtaaccg taccgccggt gccctggttc
    26221 atgatgaaga cgtccagttt gcctcccgtc tgttcccata tttccggccc ggtatgttca
    26281 taatgggcca gcagattgtc ctgcctgaca aattggccga tttcaaatat tttttctccg
    26341 tagcgggctt tgatgcgttc cagctcttcg attaccagat ccacatccgt ttccgcgccg
    26401 ggcgtcaaca ccagctgcgc accgtaagac tggatggtct ttttccgttc ctcgctcatt
    26461 ccctcgggca tgacgatgat aacctgataa cccttggctg cgcccaccat agcggtggcg
    26521 atgccggtat tgccagtggt tccctccagc aaaatcatat cgggcttgag atcgccgcgt
    26581 tgttctgcct tttccacaat tttgtgcacc acccggtctt tcagactgcc gctggggttg
    26641 acgtattcca gtttgacaca gatatcggtt cccagatcct gactccagcg atttaaacgg
    26701 atcatggggg tatagccgat ggcttccagc accccgttgg caatggtctt gttcctgttc
    26761 caatccatgt attattcctc ctcaagtttg tatcaatata ttgtaatgat tgaggctttt
    26821 tattacagcg gccattcctt gccgaaatgc agctgccgca cgattttaaa ggattgctgc
    26881 acgggcatgc tacacatgaa agccggtgtt tcttcgagtt cctgctgata ttgctgcacc
    26941 agggcgcgta aataatcgct ccgtgattgc gggttgcgca gacacaatcc ctgctggtcc
    27001 agggcatcgg ccagccgctg attaagatgg atttcataat cagtcagctg ctccatatag
    27061 tctttccact cctgcagggc cgattcctcc ccccagccaa tccccggaca gacaaaatgc
    27121 cggcagatgg actcccgcca ggtttgttcc agcagacaac caccatcttt gctgtggaat
    27181 ttgcagcggt aactgtcccc gtcgggaaca ttgttgacag tgaccgagtg atccagaatg
    27241 gtcaggtggt caaaggcaaa gatctgttca atcacatccg gctgatgaag atacaaatag
    27301 gccagatcgg tgccataaaa aaccggcgag taatgaccgc agcagccttt atcatcctga
    27361 cgggggcatt cggcgcagag atctacttct atgtaagtac gcaaacccag attatgaaaa
    27421 atgagtgtta tctctttaac cactgtcttg tccttccggg aagtattata tgcgcattgg
    27481 caatcgattc gtttcattac atttaaataa gttttcttaa gcctagctaa aagcttatag
    27541 tatttctgct ccggattcaa tcattgccgg tcctgatttg caatttctac cctgaatggc
    27601 tatcattaat ttagaggata aagcttttcg agccgacggg tacggggttt tcatcgttta
    27661 acaagcagca gggagaagta tatggcaggt acacaatcac tgggcatttt tggcggaacc
    27721 ttcgatcccg ttcattacgg acacttgctg gcagcgcaat gggccggcga agcgtttgga
    27781 ttggatgaaa tcattttcat tccggctgcc cgaccgcctc acaaagaccc ggccggcgtc
    27841 atcaatggac gacaccgcct gcgaatggtc gaactggcga tcaagaacaa tcctactttt
    27901 tccgtatctg cgctggaact ggagcgcagc ggctattctt atacggtgga taccatccgg
    27961 cattttcttc tggctgatcc gcgacttgat attcgcttta ttctcggtgt ggatgcgctg
    28021 caattgatcc atacctggaa agaagtggag caattgatta cactgtgccg cttcattgtg
    28081 gttactcgtc ccggttacga gttggaccgg gctgatccgg tctttgccac ggtgccggca
    28141 gagctttggg accggatcga tttgcttgcc attcccgggt tggagatctc ttcttccgaa
    28201 attcgcaagc gcatagctgc cggccgctcg gtgcgatatc tcttgccccc ggaagtggct
    28261 gaatatatta aaaagaacga attatacagg aatgaaacca atgatcaata gaaatgagca
    28321 tgagattctg gccatactgg aacgcagatt gtcggagcac cgcttccagc acagtgtagc
    28381 ggtagcccag gcagcccggg aactggctgc gatattcggt gaagaagaag atttggctta
    28441 taaaaccggt cttttgcatg attatgccaa agggattcct gctcctcaat taattgcgat
    28501 tgccagcgag aatggcctga tcgaggatga aatcgaaaat aaagtgccgg atctgctgca
    28561 tgcaccggtg ggagcctatt tgctgaaact tgaattgggc atagaaaatc cgacggtgtt
    28621 ggaagccgtc agggtgcata ccatgggatc gctgcatatg tcccgattgg ataaaatcat
    28681 atttctggcc gatatgatcg aacccggccg tgactatccc ggtcaggacc gcttggattg
    28741 tctggccaga agggatcttg accgggccat gttatatggt ttggatgcca ccattcgtta
    28801 ttgtctggat caggccagga ttcttcatcc ccggaccatt acggtcagaa atcatttttt
    28861 ggagattctc aggcaaccaa taaaagatga atgagtattc ccgtaagtaa ggatttagga
    28921 gggctgtttt tggaaaaaga agaacgtatg gcaagggtga ttgctgaagc agcattggaa
    28981 gaaaatgcat tggacatggt gattctcaat gttcacgaac tgactatcat cgccgattat
    29041 tttatcattt gcaacggcag gagcaccgtc cagatcagaa gcattgcgga aaatgtagaa
    29101 aaagtcatgc aggaaaagga agggttgctg cccttgcggc gggatggcct ggaacagggc
    29161 aaatgggtca tcctggatta tggttcggtg atcgtgcata tcttccggca ggaggaaaga
    29221 gattactaca aattagaaaa attgtgggga gacgcgcgtg agatttcggt caatcaggaa
    29281 atgcagtgac aagctgacaa tgccggtata accttgaaat caatcatgcc tatgacgatg
    29341 atggatgccg gtattgtagg gacaagtatt gccgagattg tatacattat atctgcgctg
    29401 caagccttgc atacttgacg ggaagcgttt aaaattaccg tgtggatgca atggagtgtc
    29461 ctcttttggg tcaactgaat attgcttagg gacaatgggg tcctcgcacc tgtcggtgtt
    29521 gaggcccctt ttattatttc tataggaaat gcgttaaagg ttcgcaaatg aaaaaagtca
    29581 ggaacaagtc tatataaata cggccaatga gggtggtaat atgaaaaatt acgggctgcc
    29641 aggcaaacag ggactgtacg atccagcatt tgagcatgat gcatgcggga tgggatttgt
    29701 agcccatatc aaaggagaaa aatcccatca gatcatagag gacgctttaa ccgtgctgga
    29761 gaatctcagt caccgcggcg caagcggcgc cgatgaaaat accggtgacg gggctggcat
    29821 catgctgcag atcccgcatg ttttctttca acgggaatgc agcgtgctgg gctttgattt
    29881 accggcaaca ggcggttacg gggtgggcat gatctttgcc catcgttatg aggatttcag
    29941 aaatacgcag atggcagcct ttgcgcagat cgtaaaggaa gaaggccaga agatcctggg
    30001 ctggagagaa gtgcccattg acaaaagcgg catcggggcc ggtgcccaat ccgttatgcc
    30061 ccgtttcatc caggttttca ttgccaggga tgagggactg tgcgatgaca tggattttga
    30121 aagaaaactc tacgttattc gcaaaagagc ggaaaaaatc attattcccc tgtgcgagga
    30181 caagggaggc tcattttata tagccagtct ttcatcaaga accgttgtct ataaagggat
    30241 gctaacggct gagcagctgc gcagcttcta tctggatctg tccgacctgg attttgcatc
    30301 agctttggcc atggtgcatt caaggttcag caccaatact ttccccagct gggaaagagc
    30361 gcatcccaac cgttatatcg tccacaacgg ggaaatcaat acggtgcgcg gcaacgtcaa
    30421 ctggatgaag gcccgccaga gggtcatcga ttcaccgctt tttgcagaca ttaccaaggt
    30481 ctatccgatc attgatgaga gcggaagcga ttccgccatg tttgacaact gtctggaatt
    30541 tctctatctt accggacggt cactgccgca cggcgtaatg atgatgatcc ctgagccatg
    30601 ggaagccaat catcttatcg ccgcagagag aaaagaattt tacaaattca acaacttttt
    30661 aatggagccc tgggacggac ctgccgcgat cggtttttca gacggaacgg tgatcggtgc
    30721 ggtattggac cgcaatggtc tgcggccggc ccgctattat gtcaccaagg acgaccgggt
    30781 gatcctggcc tcggaagtgg gcgtcatcga catcaatcag gccaacgttg cctgcaaagg
    30841 acggctcgaa cccggcaaga tgctgctgat cgataccgag gcccgcagga tcatcagtga
    30901 tgatgaaatc aaaagcgagg ttgcccggct gcatccctac caggaatgga acgggcagca
    30961 catcgtagat ctggatgata tgccggctgc ccccgcggca gaagaaactg ataaaaccta
    31021 tctgatcgag cagcagaagg cctttggcta tacgtttgag gatatttatc agaccataca
    31081 gcccatggcg gctgagggtc acgatccggt cggctcgatg gggatggact ccccgctggc
    31141 agttctgtct gaacagccgc agctgctgta cctgtatttt aaacagcttt ttgcccaggt
    31201 taccaatcct cccattgatg ggatcagaga agaaattgta acctccagca gtctgccgct
    31261 gggcagcacc ggaaacctgc tggatcccaa ccacagccag gcggcttgta tttatctgga
    31321 gcatccgatc cttaccaacg aacagcttca taacctgaaa tatttgcatg atgaccgctt
    31381 caagccggct gtgatttcca tcctgtataa agcttccgaa ggtgccagag ccatgaaccg
    31441 ggcattggaa cgtattttcc gccaagcaga tcaggcgatt gccgatggtg ccaatatcgt
    31501 tatcctttcc gatcggggcg tcaatgaaac gctggctcca attccggcgt tactggcttc
    31561 ctccggtttg catcatcatc tgacccgaaa agagatccgc accaaggtgg gcatcgtgct
    31621 ggaatcaggt gagccgcgcg aggtgcatca cttttgtacc ctggttggct acggtgttac
    31681 ggccatcaat ccctggctga cttttgacac cgtccgtcag cttggtgaac aggaagcgct
    31741 gggcggtctg agcttggccg aagcgcaaaa gaattacatc caggcttctg tgaaaggcat
    31801 gatgaaagta atcagcaaaa tgggcatttc caccatgcgg ggctatcatg gcgcacaggt
    31861 gtttgaagcg gtgggactga agcgggaact ggtggataag tattttacca tgaccccttc
    31921 ccgcctggaa ggaatcgggc tggaggaaat cgcccaggaa agcagaatgc gccacgacag
    31981 tgcctttaat agaaatgatc cgggccggga gacgttggag accggcgggt actttcaatg
    32041 caaagagaat ggagaagtac atctttacaa tccgcaaacg atttatttgc tgcagcgtgc
    32101 ctgccgggag ggcgactacc atcttttcaa ggagttctcc cgcaggatca atgaggaaaa
    32161 agtatatacc ctgagaaatt tgctggagtt taatattatt cccggtgata ccatccccat
    32221 cgaagaagtt gaaccggtgg aatcgattgt cagaaggttt aagaccggtg ccatgtccta
    32281 cggctccatc agccaggaag cgcatgaatg tctggccatc gccatgaacc ggctgggagg
    32341 taaaagcaac agcggtgagg gtggagaaga cgctgaccgt tatatcccgg ataagcacgg
    32401 agattccagg agcagtgcca tcaagcaggt tgcctccggg agattcggag tcaccagcaa
    32461 ctatctggtg catgccgcgg aaatacagat aaagatggcg cagggcgcca agccaggcga
    32521 gggcgggcaa ctgcccggcg aaaaagtcta cccggccatt gccaaggtcc ggcattcaac
    32581 ccccggagta gacttgattt ctccgccgcc ccatcatgat atctattcaa ttgaagacct
    32641 ggcagaactg atccacgatt tgaaaaatgc caaccgggat gccggcatta gtgtaaaact
    32701 ggtatccgaa gtgggggtgg ggacaattgc ggccggggtt gccaaaggca aggccgatgt
    32761 catcctggtc agcggctacg acggaggaac cggtgctgcc ccccgcacca gcatcaggaa
    32821 tgccgg
//
LOCUS       DAOTHZ010000017        31494 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1616_length_31494_cov_8.204141, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000017 DAOTHZ010000000
VERSION     DAOTHZ010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 31494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31494
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1616_length_31494_cov_8.204141"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="P5549_03560"
     CDS             complement(<1..560)
                     /locus_tag="P5549_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRW12183.1"
                     /translation="MVKDMVLYKGSSDYIVSDDLRNSVNIAIALGKPLLIKGEPGTGK
                     TVLAQSIADALNKELIIWNIKSTTKAQEGLYVYDTVQRLYDSQFGDHDVANIKQYIKL
                     GKLGEAFQSEQQVVLLIDEIDKADLEFPNDLLWELDQMTFYIPETKETISARTRPIVI
                     ITSNAEKELPDAFLRRCIFHYIAFPEP"
     gene            complement(637..1875)
                     /locus_tag="P5549_03565"
     CDS             complement(637..1875)
                     /locus_tag="P5549_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRW12184.1"
                     /translation="MKKVIVIGAGPAGMMAAGMAALGGAKVILAEKKSRVGSKLKITG
                     KGRCNITAAVDQDSLIKGLPGNGKFLYSALHQFSNNDLIKFFHERGLETKIERGNRVF
                     PCSDQARDVVEVLQHFVQQAGAEIKTSLTINRLLVENGRIRGVDSNQGFLAADAVIIA
                     TGGLSYPGTGSTGDGYRLAQDCGHSLVEPRPGLVPLVIEESWVKELQGLSLKNVDATA
                     FTAAGKKINQDFGELLFTHFGLSGPIILSMSRDIGEYIYRRQQKVILKIDLKPALNEE
                     KLDERLLRDLDKYSRKQFKNSLHDLLPQKMIPVFIALSGIDGDKPCHQISRGERLKMV
                     NLLKYFTVTVTGTRPIAEAIVTAGGISVKEVDPRTMESQRVKGLYFAGEVLDVDGYTG
                     GYNLQAAFSTGYAAGEHAAR"
     gene            2008..2568
                     /locus_tag="P5549_03570"
     CDS             2008..2568
                     /locus_tag="P5549_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12185.1"
                     /translation="MANTPRAKVPVFIWFVIVVLLAALYGYAAYWDPNRPEKTVQNFY
                     QAYFTGDYKTVSSNLSVFWAVRLLPQYADMDSVELLTKRAEIEENLFQVISEVESMYD
                     APRDISIQILKPYTKEGTYSAVVVYKFMEKSKEAGMEAAILIKEQGKFRIFTMTPVDE
                     QSLSQIKAVNISELDANFLELQSGVE"
     gene            complement(2649..4160)
                     /locus_tag="P5549_03575"
     CDS             complement(2649..4160)
                     /locus_tag="P5549_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HRW12186.1"
                     /translation="MKENSLKSNHVQDSHRLLVIATLIIFVLANIISLYLYLSGKGST
                     QLSLRLIGLEMLVISSTLAVTAAIIKYFPNRNWVKYLTVTMFGICILSFDYVMSGAPE
                     VFANFYLVMGISLIYLDMSVSLYAIILSLGLHTLLVMTVPDIVYSQNFVLSMAVRYAS
                     FIFFGIVAGFIASVVARLLKKSNAKEQQATALNEKLQYMIAGVADQADRLALSAAALF
                     DYSTQTDQSAHQVNNSVSSLSVAAAETASFTSKSAQVNRQISMALENAGNNVQVVSNQ
                     TAGFGRIVDEGINAMREQDNMMQESLEAQESVSQAVYMLGNKSQEIESIVELITAIAN
                     QTNLLSLNAAIEAARAGEAGRGFAVVAEEVRKLAENSAQAARDIARLITEIQQGIVLT
                     VKEIDHSNQLYNQQEQAVNMTRQMFSHIEEGAHSITDAIQEVSAVLEEVLSSTDEMVQ
                     NMDNVAANNEESAASIQEIAALSKQQTDEVTSIVEMARKLAESSEQLKKLVGH"
     gene            complement(4279..5283)
                     /locus_tag="P5549_03580"
     CDS             complement(4279..5283)
                     /locus_tag="P5549_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HRW12187.1"
                     /translation="MKAMIMAAGVGSRLMPMTADIPKPMIPVANRPLMENTLQLLSQH
                     GIKEVIANLHYHGDMIRDYFHDGKAWGINLLYSPEEKLLGTAGGVKKCDWFLDETFLV
                     ISGDALTDIDLGRLLEEHQRCGALATIALKPVDEVEQFGVVVTDDVGRIVQFQEKPRS
                     EEALSRTANTGIYIFEPEIFTMIPAGEFYDFGKQLFPWLVQSKAPFYGIPIGEYWCDV
                     GDIKTYRQANADILCGRVKVPWVGQLRYDDRLNAVLMGEGASLGADVTIKGQVVIGAG
                     CQIGAGTIIEDSVLWNDTVVGENAIIKESVVGSVCHIGKGVTLSPDSVVASGTYLPDK
                     "
     gene            complement(5463..7055)
                     /gene="cimA"
                     /locus_tag="P5549_03585"
     CDS             complement(5463..7055)
                     /gene="cimA"
                     /locus_tag="P5549_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRW12188.1"
                     /translation="MLDVTIYDTTLRDGAQGEGISFSLDDKMKIARKLDYLGISYIEG
                     GWPGSNPKDLEFFQAMRDYQWQHAQLTAFSSTRKAGVNVRQDSNVKALLESGVQVATI
                     FGKAWDFHVLKALETTLEENLSMVRDTIVYLKECGLQVFFDAEHFFDGFKNNPEYALQ
                     VLETAARAGAECLVLCDTNGGSLPWEISGIIDQVKQRISAPLGIHNHNDSACAVSNSL
                     IAVQQGCVQVQGTINSYGERCGNADLISIIPNLELKMNKRCLPVGGLKHLTEVAHYIA
                     EVANMPSSNSQPFVGHGAFAHKGGIHVNALLKDALTYEHIDPEQVGNHRRVLVSELSG
                     LSNLLYKARELNLDVNSYNAETRKVIKQIKELENEGFQFEGADASLELLLRKAFGQYE
                     EFYQLKNLKIIMEKNESGEIVSEAIVKLSIGEEIVHTAAEGDGPVNALDNSLRKALNE
                     AYPEINEMHLRDYKVRVLNGNDGTAAKVRVLIESANQYDQWSTVGVSENIIEASWQAL
                     VDSINYLMMKKQLAAKSAQDTI"
     gene            complement(7104..8177)
                     /gene="leuB"
                     /locus_tag="P5549_03590"
     CDS             complement(7104..8177)
                     /gene="leuB"
                     /locus_tag="P5549_03590"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRW12189.1"
                     /translation="MYKIAVLPGDGIGVEIVPEAIKALQAVGEKYGHQFQFTEALVGG
                     AAYDAVGHPLPAETLQICRQADAVLLGAIGGPKWEKLPVHLRPEVGALLPLRKELGLY
                     ANLRPCFLFPGLTHASTLKEEVITGVDLIVIRELTGGLYFGEKSREKTDEGVKATDIL
                     SYSTYEIERIVRYAYEVARKRNHKLCSVDKANVIETSRLWRETVTELAKEYPDVETSH
                     MYVDNCAMQLIRNPKQFDVIVTENMFGDILTDEASMLTGSIGMLPSASIGGEVGLYEP
                     SHGSAPDIAGQNKANPLATILSAAMLLRYSLNLEKEAQIIEKAVSVVIEKGYRTPDLL
                     EDGCIPVGTDEMGDLVVRQIKSL"
     gene            complement(8222..8716)
                     /gene="leuD"
                     /locus_tag="P5549_03595"
     CDS             complement(8222..8716)
                     /gene="leuD"
                     /locus_tag="P5549_03595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRW12190.1"
                     /translation="MKFNGQVYKFGNDIDTDAIIPARYLNTFDPDELARHCMEDADPA
                     FPQKVKAGDIIVAEKNFGCGSSREHAPIAIKAAGVSCVIAKSFARIFYRNAVNIGLPI
                     LESAEAVDSIQEGDQLEVELETGRILNLRTGDSYQAVPFPEFMRQIMDKGGLIEYVRD
                     KIAE"
     gene            complement(8775..10034)
                     /gene="leuC"
                     /locus_tag="P5549_03600"
     CDS             complement(8775..10034)
                     /gene="leuC"
                     /locus_tag="P5549_03600"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRW12191.1"
                     /translation="MGMTISQKILAAHAGREYLEPGELINCKLDLVLGNDVTAPVAIS
                     EFNRLGLQEVFDQERVVLVPDHFMPAKDIKSAEQAKTVRDFARGFNIKNYFEIGAMGI
                     EHCLLPEQGLVGPGDLVIGADSHTCTYGALGAFATGVGSTDMAAAMATGEAWFKVPES
                     IKFVYYGKKPQWVGGKDFILRAIGDIGVDGARYMSMEFCGEAISNLSIDNRFTMANMA
                     IEAGGKNGIIAPDDITLAYVRGRAQRPYTLYQSDPDAVYAEVKEYDVSQMEPLVAFPH
                     LPENTRPVSEATDVLIDQVVIGSCTNGRIEDLQTTAAILKGHKVHPEMRVIIFPGTQE
                     IYLEALRRGYIETFVEAGAVVSTPTCGPCLGGHMGILAKGERALATTNRNFVGRMGHP
                     ESEVYLCNPAVAAASAIKGRITAPWEI"
     gene            complement(10062..11600)
                     /locus_tag="P5549_03605"
     CDS             complement(10062..11600)
                     /locus_tag="P5549_03605"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRW12192.1"
                     /translation="MSDKNRIYLFDTTLRDGEQSPGVSLNVEEKVEIAQQLARLGVDI
                     IEAGFPIASPGDFQAVKTIARNVKGPVIAGLCRATRADIDRTWEAVKYAENPRIHTFL
                     ASSDIHLQYKLQKSREEVLAQAVEAVKYARQYCSDVEFSAEDATRSDLDYLVQLVEAV
                     ITAGAKVVNLPDTVGYTVPDEFGHFMKTVIDRVPNSDQAIFSVHCHDDLGLAVANSLA
                     GVYNGARQIECAVNGIGERAGNASLEEIVMALYTKHAFYQKEVNVKYEEIYRTSRLVS
                     TLTGMVVQPNKAIVGSNAFSHESGIHQDGVLKERTTYEIMSPELVGVFKNNLVLGKHS
                     GRHAFKARMMELGYDLSDEELQRAFEKFKDLADKKKEISNEDIEALIKDQGRSIPERF
                     SLEYLHISSGTNMIPTATVRLRIDNMPFESAATGSGPMEAACHAVDKITDIYGKLLEY
                     KLHAVTGGKDALGEVLSKVEIEEKIYNGRGLSTDIIEASVKSYIHAINKFYYEQQLEN
                     SQQN"
     gene            complement(11642..12676)
                     /gene="ilvC"
                     /locus_tag="P5549_03610"
     CDS             complement(11642..12676)
                     /gene="ilvC"
                     /locus_tag="P5549_03610"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRW12193.1"
                     /translation="MAKMYYDADANLELLKGKTVAVMGFGSQGHAQAQNLHESGVNVV
                     VGLRKPFNEASQAEWDRVIAAGIQPMTVAEAARQGDIIQILLPDEAQAKVYREEIAQY
                     LTEGKALVFSHGFNIHFGQIVPPANVDVFMIAPKSPGHLVRSEYVKGAGVPALIAIQQ
                     NYTGQAKELALAHAKGIGATRAGVLETTFQEETETDLFGEQCVLCGGVTQLVKTGFET
                     LVEAGYQPEIAYFECFHELKLIVDLMYEGGMSNMRYSVSDTAEWGDYITGPKVIGEEA
                     RYAMQEALAEIQDGKFAKEWLLENQVGRPQFNALKRQGREHLIEKVGAELRAMMPWLK
                     SGNALVKENK"
     gene            complement(12701..13183)
                     /gene="ilvN"
                     /locus_tag="P5549_03615"
     CDS             complement(12701..13183)
                     /gene="ilvN"
                     /locus_tag="P5549_03615"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRW12194.1"
                     /translation="MAKHTLSVTVENRPGVLTKVATLFRRRGYNIDSLTVGRTESPDI
                     SRMTIVVEGDDRIIEQVNKQLHKLVEVIKIIDITQEKAVERELSMFKVRADNTVRAEI
                     VQIVDIFRARIVDIGKNSLIIEVTGDENKIDAIEESLRPFGIIEMVRTGKVAMVRGNK
                     "
     gene            complement(13176..14867)
                     /gene="ilvB"
                     /locus_tag="P5549_03620"
     CDS             complement(13176..14867)
                     /gene="ilvB"
                     /locus_tag="P5549_03620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRW12195.1"
                     /translation="MKLRGADILLRCLQAEGVEVVFGYPGGQAITIYDALYNSPLKHV
                     LVRHEQGAAHAADGYARVTGKTGVCIATSGPGATNLVTGIANAYMDSIPMVVITGQVP
                     THMIGRDAFQEADITGIVMPITKHSFLVERTEDVAQVVKEAFYIANTNRPGPVLIDLP
                     KDTMEREIEFDGPAKEMNIRGYRVLKGYNTGQVLTAADLINSARRPVIYAGGGVVASN
                     AADELLAVAEKMQIPVTTTLMGMGAFSGYHHLYLGMLGMHGTRYANYAIGECDVLLAI
                     GVRFDDRVTGKIAEFAPHAKVIHMDIDAAEIGKNVCVDVPIVGDVKEILQGLLPRLVP
                     AGNLREWHETIERWKDENPLSYAGSVEGRIPPQHIIEKISDLTNGEAIITTEVGQHQM
                     WASHYYKFRHPRSFVTSGGLGTMGFGFPAAIGAKVARPDLPVIDIAGDGSIQMNIQEL
                     GTAVQHKLPVIICILNNQYLGMVRQWQTLFYGERYSHTDMTCQPDFVKLAEAYGAVGM
                     RVKCIEEVDEALQKALQVTDGPVIIDFWVDRNTNVYPMVPPGEALNNMLGGEVDG"
     gene            complement(15039..16700)
                     /gene="ilvD"
                     /locus_tag="P5549_03625"
     CDS             complement(15039..16700)
                     /gene="ilvD"
                     /locus_tag="P5549_03625"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRW12196.1"
                     /translation="MKSKIVKEGLEKAPHRSLFKALGITDEEMKRPFIGVVNSWNEIV
                     PGHINLDKISDAVKAGIRMAGGVPFEFHTIAVCDGIAMNHIGMKYSLGSRELIADSVE
                     IMAIAHAFDGLVFIPNCDKVVPGMLMAAARLNIPSIFISGGPMLAGNMAEKKVSVTQV
                     FEGVGAAASGKINTEELSRLEENACPSCGSCSGMFTANSMNCLTEALGMGLPGNGTIP
                     AVYSERIRLAKTAGAKILELVEKDLRPADIMNAAAFHNAMSLDMAIGCSTNSVLHLMA
                     IANEAGVPISLQKFNELSAVTPHLCKISPAGNHHMEDLYRAGGVQALFNELDKKGLIH
                     CNAMTVTGASVGDNIKTAPVLDYSVIRSVNKPHSESGGLSILFGNLAPEGAVVKKGAV
                     DPEMLQHQGPARVFESEEEAVEAILANKIKAGDVIVIRYEGPRGGPGMREMLTPTSTI
                     AGMGLDKEVALITDGRFSGATRGASIGHVSPEAAAGGNIALIEEGDLIRIDIPALKLE
                     LLVEDDVLEARRASWQEPELKIKTGYMKRYAQMVQSASTGAVFVK"
     gene            complement(17118..19109)
                     /locus_tag="P5549_03630"
     CDS             complement(17118..19109)
                     /locus_tag="P5549_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12197.1"
                     /translation="MKNNNGYYTKQVLQQRAEEINNYIDSNWGKLLLLEIFEENPLDL
                     RENFLEGLGSLYSPQMASYFQLINEEYGLEYGVLCQRILNKYRLAGIKTADEATFDYS
                     FYKAYVSCSRHTGRISLDVAWYKDQGNLYVECFFLTFNPDGIYSLFVVEDIAQSQYEK
                     DHGLMADITEIDYSEACYLIAEAYKLNVRYMSRPALGKFIYQRYLDEPLELTADQVQN
                     LNKRISARLAPRQLGNSLFHALRYKDYNYLLSILDTQLISGKLFYQFKEAINPGAFIM
                     EGQVEEARASGEHAHLRAFSVILQEREVYKSEYKIALKRDPQGYWSIIDIKQVSHQLI
                     DPESVWNPFALQVFCRVYEITEMDELFDVLDRVENIREVEELPYGIHMRLTCYEDDFN
                     HGVSFMTGVIADLIINMDEFVIISQEQEVVDQFHKIFCEGSYPFLVQRGEYEITLADA
                     YRFLSGQYNNFEDILLGDENDLVYEDGMRFVSVRYFVKDRTKVLHRLEKLNAETIETY
                     DDIKIYYELDDGFDPANFRAEYMMGSNWLTVSAFGEKEMNYIRQGFEENMYEALEFDG
                     LEVREEGLFSILDMEVKKRYPDLEATLKEMYLNKWYHSHLHGLRGMSPSEACQSEEGT
                     RLLWSMLKKIKLKEKKRQMNGFSYRIGVKEYLRKIDLRK"
     gene            19274..19828
                     /locus_tag="P5549_03635"
     CDS             19274..19828
                     /locus_tag="P5549_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HRW12198.1"
                     /translation="MLWEMPGSDNTRETLQLAVKRSNELSITHFVVASCSGATARELY
                     ALVQEKKMVCVSHVVGFAEPGVDEMLPENRAFFNAHGVPVLTTAHLLAGIDRALRYQF
                     KGVYPAEIVASTLRMFGQGIKVAVETSCMALDAGMIPYGQDVIAIGGTVAGADAAIVV
                     RPAHSTHFFATQIKEIICMPKNKK"
     gene            19947..20075
                     /locus_tag="P5549_03640"
     CDS             19947..20075
                     /locus_tag="P5549_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12199.1"
                     /translation="MQRKSSMKFENLMVPMAFVNGLFLGGYALRMARKYWEDGPQD"
     gene            complement(20158..22143)
                     /locus_tag="P5549_03645"
     CDS             complement(20158..22143)
                     /locus_tag="P5549_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HRW12200.1"
                     /translation="MAKNKTKMASFLFVLLLIMLGVLAGTALFPEKQNPVAVARADKE
                     LPVVGSWENLRILLEQYEQKNAYMYREMAVDESIPTNMKSQAAMPAAGQADDYSATNV
                     QVEGVDEGDQVKTDGQYIYQINHNQVQIIKAVPVEQMEIINTIRFNDQNFSPVEIYID
                     GDYLIVIGNTYIPEPVYPVDQSQLRMKSAAMPPYYHRNASLCRTIVYDMKDKRNISQI
                     RRLVLEGSYLTSRLVDSKLYLLSNCHVDYYSIQKGLETTPMCKDTIKADRFDVQDIKT
                     IRYFPDCITPNYLVAGAIDLQQMDKPAAINTYLGGGENVYASTQNLYVAASQYFNNVI
                     RSTLSTAPASPADTTTKIFKFKMKDAQMQYAGEGQVPGTILNQFSMDEYNDTFRIATT
                     YGDVWRDDQYTSQNNVYMLDRDLKLIGQIENIAPGERIYSTRFMGHRAYMVTFKDVDP
                     FFVLDLTDPAQPKILGKLKIPGYSDYLHPYDENHIIGFGKETIELSGYNEKSMAFYQG
                     LKIAVFDVTDVNQPVEMSKVVIGDRGSDSELLRNHKALLFSREKNLLALPVTVMEIGQ
                     KNAKQNNANIPEYGRFSFQGAYVYHIDLQQGLQFKGRITHLDQDDYLKAGNYWDNPDK
                     SINRILYIGDALYTLSPAMIKANQMDSLQEKQVLRLQ"
     gene            complement(22230..22619)
                     /gene="dut"
                     /locus_tag="P5549_03650"
     CDS             complement(22230..22619)
                     /gene="dut"
                     /locus_tag="P5549_03650"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRW12201.1"
                     /translation="MDKIEVKFKKLVSWAKLPEYETRGAAGCDLAIAIQEEIILYPNE
                     VRSFPTGFAMMIPKGYEAQIRSRAGMAGKGIVVANAPGTIDCEHTGEVKILLLNITDK
                     PLRILPGQKVAQMVFAPVVQASFSEGS"
     gene            complement(22856..23620)
                     /locus_tag="P5549_03655"
     CDS             complement(22856..23620)
                     /locus_tag="P5549_03655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRW12202.1"
                     /translation="MKYVVKTIGILMAAILLLGLLKSQLYAIQTFTQEDSEMSRVIYV
                     VDSNLNKRLITHADTGGKKRLALTFDDGPDPLYTGAVLEILQKFDVQATFFVVGENAA
                     AHPELIQQEIEAGHEIENHTYTHADLSLADTAGTEAEIQKTDQLLSVMLGRKSKYFRP
                     PKKLFRPETVEIAERNGYQTVLWTICVENARSPTPAAMAKRVIEAARPGMIILAHDGR
                     LDRRQTLLALPIIIDAYQKQGYQFVTLDELLQTIDA"
     gene            23803..24975
                     /locus_tag="P5549_03660"
     CDS             23803..24975
                     /locus_tag="P5549_03660"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRW12203.1"
                     /translation="MQNPAIPARLAGITSFIVMDVLEKAQSMEAQGIDVIHLEIGEPD
                     FSTPEAVKDAALKAIREDDTHYTHSLGKLSLREEIAGYYWRKYGVKISPEQVMITSGT
                     SPAMLMIFSVLLEGGKEIIMPDPYYSCYPNFVRLTDGKSVLVPVYEEDGFIYRQETVR
                     QLIRPSTRAILVNSPSNPTGVVYPSAMLQSLADTGQMIVSDEIYHGLVYAGQEHSILE
                     YTDRAFVINGFSKLYAMTGWRLGYLIAPPAYMRTLQKLQQNLFICASSFAQEAAIIAL
                     RNCEQVVEKRRLIYDERRQYLLTRLRKMGIAPAVEPTGAFYTLANVKKYTRDSYQFAF
                     EILEKAHLAVTPGIDFGANCEGYIRISYANSLENIQEGMDRLESFLAGCTLLSAEF"
     gene            complement(25040..25192)
                     /locus_tag="P5549_03665"
     CDS             complement(25040..25192)
                     /locus_tag="P5549_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HRW12204.1"
                     /translation="MQKIKCSVANCAYNENYQCNAKGIEVNALGDGLANSSDGTCCET
                     FVPREY"
     assembly_gap    25197..25296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25501..26043)
                     /locus_tag="P5549_03670"
     CDS             complement(25501..26043)
                     /locus_tag="P5549_03670"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRW12205.1"
                     /translation="MDEQNLNANENNPTTTTLDHEPEQYYRVAGFWIRTLAFFIDLGV
                     IACLNGILFHILGPAQTEHTSVYKFIRLNDLFLGITGAAYFIIMTRYFQQTLGKMIAG
                     IKVIQAGDQPLTWQAVVFRELVARSLSQMLGLYLGYIFCWFNRDKQCLHDMLSDTWVV
                     HSSPRTDYKSESLRYSPKPA"
     gene            complement(26059..27051)
                     /gene="sppA"
                     /locus_tag="P5549_03675"
     CDS             complement(26059..27051)
                     /gene="sppA"
                     /locus_tag="P5549_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRW12206.1"
                     /translation="MNKKRWWALGIFLVLIFVYMSGSFAGIEEEKQATSALTRYIGGN
                     EGNWKTTVYQEGSGGKLALLKLEGVIMENNDSSMGLTSTYNHRYFLKQLEAAYADPSI
                     KGIILQLNSPGGGVYESDEVYHRIMELKAQYKKPLVVYMSQQAASGAFYIAMAADKIY
                     ANRNTLTGSIGVIISTYNFSELAKKIGVQDVSFKSAANKDLLNPLRPITTEEQTILQG
                     IVDESYNYFVDIVVEGRQMNREEVLKLADGRIYSGAQAKQLGLIDELGYLQDAIEGTA
                     KITKTNDPQVLLYENPGPDFLNWIMSVRAPAFDLKELSEQLQQQSAPQIMYLSH"
     gene            complement(27161..>27616)
                     /gene="rmuC"
                     /locus_tag="P5549_03680"
     CDS             complement(27161..>27616)
                     /gene="rmuC"
                     /locus_tag="P5549_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRW12207.1"
                     /translation="IHDKYLSPPHTTDFGIMFLPTESLYAEVVKRPNLLEGLQRDTRV
                     IVTGPSTMAALLNSLAIGFHTLAIEKRSSEVWALLGAVKTEFGKFGDMLEKTHKKLTE
                     ASHSLETASRKSRTIEKKLRQVQDIPQSDLQNLSDMDILEAGEDNKENE"
     assembly_gap    27617..27716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27717..28294)
                     /locus_tag="P5549_03685"
     CDS             complement(<27717..28294)
                     /locus_tag="P5549_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRW12208.1"
                     /translation="MEKDYLIISAVAILLIAFIIFNQYRLKAKLEQQDISNRVSQWLQ
                     QQEMLEKNLESLGGVLRSEMVQLRQENSRNARDNREESAQSLAVFQNVVLARMTEIAG
                     LQKDQLELFARQLGTITQNNDTRLEAVRQTVEDKLKTLQEDNRRQLEQMRATVDEKLH
                     KTLEQRLGESFRQVSERLEMVHKGLGEMQTLAA"
     gene            28543..29451
                     /locus_tag="P5549_03690"
     CDS             28543..29451
                     /locus_tag="P5549_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12209.1"
                     /translation="MLYSVGKDIYLVAPRVPRYPYSNSLYIEDERPMVIDFGAGGTAF
                     VDVPHEKIELGLISHFHFDHIHSRSLFPNIALMAGAEEKFTYVDQNAYIHFHGYDLWD
                     ELMHIEREMYGDVIKIPRDIPIEPGFKTIHLADTFSDGDVIQTGKYEVMTVHLPGHSA
                     GHYGFYLEKENILFSADIDLVKTGPWYNSNSGSVGDLIASVQKIKDLSPRMVIPSHRR
                     IQTENLVSQLDAYIQVVLDREEKIYNYLHQPHTIDQLATYRLTYPQQRDQYEAFWEKM
                     TIRNHLRHMLELGLIKEIEPGVYQQI"
     gene            29662..31032
                     /locus_tag="P5549_03695"
     CDS             29662..31032
                     /locus_tag="P5549_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRW12210.1"
                     /translation="MEAFSALISTVNGFVWGPWMLVLLVGTGIFLTLRLGFLQITQLP
                     YALKLAFSPSQQDKISKGDISHFQALMTALAATIGTGNIVGVATAVVLGGPGAVFWMW
                     ITALFGMATKYGEAVLAIKFRVTDEKGEMAGGPMYYIEKGLGWKWLAVLFAIFGSVAA
                     FGIGNLVQANAVAGNMHDTFGISPVITGLILAVFTALVILGGVKSIGRWTGIIVPFMA
                     VFYVLGGLYIIFANFHMVGPAFAIIFESAFSAEAGFGALVGTTIRFGVARGVFSNEAG
                     LGSAPIAAAAAKTDHPGRQGLVSMTGTFLDTIVVCTITGLSLVMANLYIGVEKAGAAA
                     LTSQSFQHFIPFGNFIVTIALLFFAYSTVLGWSYYGEKCFYYLVGQKGIVPYRVFFCV
                     LVFIGAILKLGLVWDIADTFNGAMAIPNLIALLALSGVIAAETKEFKQIRMKEKAEAR
                     AQKMAG"
BASE COUNT         7827 a         7892 c         6789 g         8786 t
ORIGIN      
        1 ggctcaggaa aagcaatgta atggaaaata catcttctca ggaaagcatc cggcagttcc
       61 ttttccgcgt tgctggtgat gattacgatc ggtctggtcc tggcactgat ggtttccttg
      121 gtctcgggga tatagaaagt catctgatcc aattcccaga gcaagtcgtt gggaaattcc
      181 agatcggcct tgtcaatctc atcgatcagg agcaccacct gttgctccga ttgaaaggct
      241 tcacccagtt tgcccagctt tatgtactgc ttgatgttgg ccacgtcatg gtctccaaat
      301 tggctgtcat acaggcgctg gaccgtgtca tatacataaa gtccttcctg ggctttggtg
      361 gtggacttta tattccaaat aatcagttcc ttgttcaggg catcggcgat gctctgagcc
      421 aagaccgttt tgccggtacc aggttcgcct ttgattaaca aaggtttgcc cagcgcaatg
      481 gcaatattaa cactgtttct caagtcatca gatacgatat aatcggatga acccttatat
      541 aaaaccatat cttttaccat atttacaatt cctccagtca gtttgcttta actatgcaat
      601 gatcaaacta catagtagca gatgaaacaa ggcaagtcaa cgcgcagcgt gctcgcccgc
      661 tgcatagccg gttgaaaaag ctgcctgcaa attatatcct ccggtatatc catccacatc
      721 cagtacttcg ccggcaaaat aaagtccttt gacccgttga gactccatgg tccgcggatc
      781 aacttctttg acgcttatcc ccccagccgt aacgatggcc tcggcaatgg ggcgagtacc
      841 ggtcaccgta acggtaaaat atttgagcag attgaccatc ttaagccgct ccccccggct
      901 gatctgatgg cagggtttgt caccgtcaat gccggacagg gcaataaaaa ccgggatcat
      961 cttttgcgga agaagatcat gcaaactgtt tttaaactgt ttacgggaat acttgtccag
     1021 atcacgcaac aatctctcat ccagcttttc ctcatttaac gccggtttaa ggtcgatttt
     1081 cagaatgact ttttgctgcc ggcgataaat atattcgcca atatcacgac tcatggacaa
     1141 aatgatcgga cccgacagcc cgaaatgagt gaaaagtaac tccccaaaat cttgatttat
     1201 tttcttgccg gcagcagtaa aagctgtcgc atctacattc ttcagactca acccctgcaa
     1261 ttccttgacc cagctctctt cgatcaccag cgggaccaga ccgggccggg gctcaaccag
     1321 ggaatgccca caatcctgcg ccagcctgta cccgtcaccg gtagagccgg ttccggggta
     1381 ggaaagccct ccggtggcga tgattactgc atctgccgcc aggaatccct ggttgctgtc
     1441 cacccccctg atacgcccat tttccaccag caagcggttg attgtaagcg aggtttttat
     1501 ttccgcgcca gcctgctgta caaaatgctg aagaacttcg accacatccc gggcttgatc
     1561 agaacaagga aatactcggt tgcctctttc aattttggtt tccagcccac gttcatgaaa
     1621 aaatttgatc agatcgttat tggaaaactg gtgcaatgca ctgtatagga attttccgtt
     1681 gcccgggagc cctttgatca ggctgtcctg atctacggca gcggttatat tgcagcgccc
     1741 tttgccggtg attttcagtt tgcttcccac ccgggacttt ttttccgcca ggattacttt
     1801 agctcccccc aaagctgcca ttccggcagc catcatcccg gccggtccgg ctccaatcac
     1861 gattactttt ttcatgtccc gtccgcctcc atcaagcaac attaatccaa aaacacggca
     1921 tcatattcgt attatgcttt gttaatccat tttaaaatag tacaatatat ctgtatgatt
     1981 tggaaataga aagcgaggta aacagttttg gcaaacacac cccgggcaaa ggtaccagtt
     2041 tttatatggt ttgttatcgt agtattattg gccgctttat acggttatgc agcttattgg
     2101 gatcccaacc gtcctgaaaa gacggtacag aatttttacc aagcttattt cacgggtgat
     2161 tacaagacgg tgtccagcaa tctcagcgtt ttctgggcgg tgcgtctgct gcctcagtat
     2221 gcggatatgg attctgttga attgttgacc aagcgggcag aaatagaaga aaatctcttt
     2281 caggtaatca gtgaagtgga atcgatgtat gacgcgcccc gggatatttc gattcaaatc
     2341 ttgaagccct ataccaaaga aggtacgtac agtgccgtgg tggtttacaa gtttatggag
     2401 aaatccaagg aagcaggcat ggaagctgcc attttaatca aggagcaagg taaattcagg
     2461 attttcacca tgaccccggt cgatgagcaa agcctctccc agatcaaggc agtgaatata
     2521 agtgaattgg atgccaattt tctggaactg cagtccgggg ttgaataagc ggggatcatt
     2581 tctatgaaaa aagcggcttt tcgagttgtc ttcggaaagc cgcttttatg ttcataaaag
     2641 gaagattgtc aatgaccaac cagtttttta agctgttctg atgactcggc caacttccgg
     2701 gccatttcaa caatagaagt cacctcatcc gtctgttgtt tggaaagcgc tgcgatttcc
     2761 tgaatgctgg ccgcagactc ctcgttgttg gcagcaacat tgtccatgtt ctgaaccatt
     2821 tcatccgttg aactcagtac ttcctccagc acagctgata cttcctggat ggcatccgtt
     2881 atactgtgag caccttcttc aatatgactg aacatctggc gggtcatatt gacagcctgc
     2941 tcctgttggt tataaagctg gttggagtgg tctatttctt ttaccgttaa aacgatacct
     3001 tgctgaattt cagtaatcaa cctggcaata tcacgggctg cctgggctga gttctcggcc
     3061 agttttctca cttcctccgc caccaccgca aagcctcgtc cggcttcgcc ggctcgggca
     3121 gcctcgatgg cagcattcaa ggatagtaaa ttggtctggt tggcaattgc cgtaatcagt
     3181 tccacgatgc tttcaatctc ctgcgacttg tttcccaaca tatacaccgc ttggctaact
     3241 gattcctggg cttccaggct ttcctgcatc atattgtctt gttcgcgcat ggcattgatg
     3301 ccttcatcta cgattcgccc aaaaccggct gtctgattgc tgacgacctg cacattattg
     3361 cccgcgttct ccaaggccat ggagatctgg cgattgactt gagcactttt gcttgtaaag
     3421 gacgctgttt ccgcagcagc tacagacaaa ctggatacgc tgttgttgac ttgatgggct
     3481 gattggtccg tctgggtcga atagtcaaac agggcagccg cagacaaggc caaacgatcc
     3541 gcttggtctg ccacaccggc aatcatatat tgcagttttt catttaatgc ggtcgcctgt
     3601 tgttcttttg cattcgattt tttcagaagc cttgctacta ccgaggcaat gaatccggcc
     3661 acaattccaa aaaagataaa acttgcataa cgtactgcca ttgacaaaac gaaattttgc
     3721 gaataaacga tatcaggaac agtcattacc aacaatgtat gtaatcccaa ggaaagaata
     3781 attgcataaa gagaaacact catatccaaa taaatcaaac tgatccccat gaccagatag
     3841 aaattggcaa acacctctgg tgcgccgctc atgacataat caaacgatag gatacatatg
     3901 ccaaacatgg tcacagtcaa atacttgacc caattacggt tggggaagta tttgatgatt
     3961 gccgctgtta cggcaagcgt tgaagatatg accagcattt ccaggccgat cagccggagg
     4021 ctaagttggg tcgatccctt gccgctcagg taaagatata aggatatgat attggccagg
     4081 acaaagatga taagggtggc aatgaccaac agacgatgac tgtcttgtac gtggtttgat
     4141 ttcaaactgt tttccttcat ttttacccct ctcctgacag cagacaacaa ctgtaagtta
     4201 aattctgata taactatggc caatcatgat gtaatatata ttgtccaaaa tattacaaat
     4261 tcctgcctga gcgagaaatt atttatccgg caaatacgtt ccgctggcca ccacactgtc
     4321 ggggcttaaa gtgacaccct tgccgatatg acagaccgag ccaactacag actcctttat
     4381 gatcgcattt tcccctacca cggtatcatt ccacagcacc gaatcttcaa tgattgttcc
     4441 tgctccaatc tgacatcccg ctcctatgac cacctgtcct ttgatggtaa catcagcacc
     4501 aagacttgcc ccctcaccca tcagcacggc gtttaaccga tcgtcgtaac gcaactggcc
     4561 cacccaggga acctttaccc gtccacataa aatatcggca ttggcctgac ggtaggtttt
     4621 gatatcacct acatcacacc agtattcgcc aattggaatc ccataaaaag gtgccttgct
     4681 ttggaccagc caaggaaaaa gctgtttccc aaaatcataa aattcaccgg cgggaatcat
     4741 agtaaatatt tcaggttcaa aaatataaat accggtattg gctgttcggc ttaaagcttc
     4801 ttcagagcgt ggcttttcct gaaattgaac aatccggccg acatcatcgg taaccactac
     4861 cccaaattgt tccacctcat ctaccggttt caaggcaatc gtagccagcg caccgcaacg
     4921 ctgatgttct tccagtaacc tgcccagatc gatatcggtc agcgcatccc cgctgatcac
     4981 taaaaatgtc tcatccaaaa accagtcaca tttttttacg ccaccagccg ttcccaacaa
     5041 tttttcttcc ggcgaataaa gcaggtttat tccccacgct tttccatcat ggaaataatc
     5101 ccggatcata tctccatggt aatggaggtt ggcaatcact tccttgatgc catgctggct
     5161 taacaactgc aaggtattct ccatcaacgg ccggttggca accggaatca ttggtttggg
     5221 aatgtctgct gtcatcggca tcagtcgtga acccactccc gctgccataa tcattgcttt
     5281 cattttttac ctccctgatg attgtccgta agtataaaac aaattggctg tttcttttat
     5341 tatagaaaat ctgggcttat taagccatca actgtttcat ataattaact cgagttatgt
     5401 cgaatcgacc tcataacaaa accacccgga tcaaagtcaa tccaggtggt cctaaataat
     5461 ttttaaatag tatcttgtgc agacttggcg gctaattgtt ttttcatcat gagataatta
     5521 atgctgtcca ccagagcctg ccaggaagct tcgatgatgt tctccgagac ccccactgtg
     5581 ctccactgat catactgatt ggccgattcg atcaaaactc tgaccttggc agccgtaccg
     5641 tcattgccgt tcaatacccg taccttgtaa tcgcgcaaat gcatttcatt gatttccgga
     5701 taggcttcgt taagcgcttt gcgcagcgag ttgtccaggg cgttgaccgg tccgtcacct
     5761 tcggcagcgg tgtgaacgat ctcctctcca atgctcagtt tgacaatggc ttcggatacg
     5821 atttcaccac tttcattctt ttccataatg atttttagat ttttaagctg atagaattct
     5881 tcatattgcc caaatgcttt gcgcaagagc agctccaggg aagcatccgc accttcaaac
     5941 tgaaaacctt cattttccag ttccttgatc tgtttgatta ctttacgggt ctccgcatta
     6001 taactgttga catcgagatt taattcccgg gccttgtaaa gaaggttgga gagtcccgat
     6061 aattctgaca ccagaacccg gcgatgattg ccgacttgct caggatcgat gtgttcatag
     6121 gtcaacgcgt ccttgagcag ggcattcaca tgaatacctc ccttgtgagc aaaggcccca
     6181 tggccaacaa aaggctgact gttgctgctg ggcatgttgg caacctcggc aatgtaatgg
     6241 gcgacttcgg ttaaatgttt gagtccgccc accggcaggc atcttttgtt catttttaat
     6301 tccagattgg gaataatgga aatcaaatca gcattgccac agcgttcgcc ataactgttg
     6361 atggtgcctt gcacctgcac gcaaccttgt tgtacggcaa tcagtgaatt gctgaccgcg
     6421 caggccgaat cattatggtt atgaatccct agcggggcgc tgattctctg tttgacctgg
     6481 tcgatgatgc cgctgatctc ccaaggcaga ctgccgccat tggtatcaca caataccaag
     6541 cattcagccc cggcccgagc cgccgtttcc agtacttgca gcgcatattc gggattgttt
     6601 ttaaaaccgt caaaaaaatg ttcggcatca aaaaacacct gcaatccgca ttcctttaag
     6661 taaacaatag tatccctgac catggacaaa ttttcttcca aagtcgtttc caacgctttc
     6721 aagacatgaa aatcccaagc cttgccaaaa atcgtggcaa cttgcacgcc ggattcaagc
     6781 agcgctttga cattactgtc ctgccggacg ttaacccccg ctttgcgggt agaactgaag
     6841 gcagtcaatt gggcatgctg ccactgataa tcccgcatgg cctgaaaaaa ctccaaatct
     6901 ttgggattac ttcccggcca tccgccctca atataggaaa tgccaaggta atcaagtttg
     6961 cgggcaattt tcattttgtc gtccaaggaa aaggaaatcc cttctccctg tgcgccatcc
     7021 cggagggtag tgtcataaat cgtaacgtct aacatcgtat tcccaatgaa gtttcctccc
     7081 caaccttact tgacattttt ctattacaaa ctttttatct ggcgtacaac cagatcaccc
     7141 atttcatcag tgccaaccgg aatacaaccg tcttccagca gatccggagt gcgataaccc
     7201 ttctcaatga caacactaac agctttctca atgatttggg cttctttttc caaattcagc
     7261 gaatatctga gcagcatggc cgcagacagt attgtggcca gcggattggc tttattctga
     7321 ccggcaatat caggagcgct gccatgggaa ggctcataaa gcccgacttc tcctccgatg
     7381 gaggcactgg gcagcatgcc gatggatccg gtcagcatgg aagcttcatc ggttagaatg
     7441 tcaccaaaca tgttttccgt tacgattaca tcaaactgtt tggggttgcg gattagctgc
     7501 atggcgcaat tgtctacata catatgcgat gtttccacat ccggatattc tttggccagc
     7561 tcggtcacgg tttcccgcca cagtcgggac gtttcaatca cattggcttt atcgactgaa
     7621 cacaatttat ggttgcgttt gcgagccact tcataggcgt agcgcacaat gcgttcgatt
     7681 tcataggttg aataactcag aatatcggtg gctttgacac cttcgtcggt cttttcccgg
     7741 gatttttctc cgaaatagag gccgcccgtc agctcccgaa tcacaatcaa gtcaaccccg
     7801 gtgataacct cttctttcaa agtggaagca tgggtcaagc cggggaacaa aaaacaaggc
     7861 cgcagattgg cataaagacc aagttccttg cgcaagggca acagcgcccc tacttcggga
     7921 cgtaaatgga ccggcagctt ttcccatttg ggcccgccaa tggctcccag caaaacggca
     7981 tctgcttgcc ggcaaatctg cagggtttct gcgggcagcg gatggcccac cgcgtcatag
     8041 gctgcgccgc ccaccaacgc ttctgtaaat tgaaactggt gaccatattt ttctcccacc
     8101 gcctgcaatg ctttgatggc ttcgggtaca atttccacac ctattccgtc acctggcaaa
     8161 actgcaattt tatacatcaa atttatctcc ttccaagcca ttgtaatgcc atagaccgat
     8221 tttattcggc gattttatcc cgtacgtatt caatcaaacc gcctttgtcc atgatctggc
     8281 gcatgaattc cgggaaaggt acagcctgat agctgtctcc ggtcctcaaa ttcaaaatcc
     8341 tgccggtctc cagttctact tccaactgat ccccttcctg aatggaatca accgcttcgg
     8401 ctgactccaa aatgggcagg ccaatattga cagcattgcg gtagaagatc cgggcaaacg
     8461 atttggcaat cacgcaggat acgccggcgg ctttgatggc gatgggggca tgctcgcggg
     8521 aactgccgca gccaaagttc ttttccgcca cgatgatatc gcccgctttt actttttgcg
     8581 gaaaagcagg atcagcatcc tccatgcaat gccgggccaa ttcgtctgga tcaaacgtat
     8641 taagatatct cgccgggatg atcgcatcgg tatcgatgtc attgccaaac ttgtaaacct
     8701 gtccattaaa cttcataaaa acaatcctcc tggttcaaac tcataacatc cgccccggtt
     8761 acgtttttca ttctttatat ttcccaggga gcggtgattc ttcccttgat ggcactggcc
     8821 gctgccacgg ccggattgca caaatacact tcactttccg gatgtcccat acgtcctaca
     8881 aaatttcgat tggttgttgc cagggccctt tctcctttgg ccaggattcc catatgtcct
     8941 cccagacacg gtccgcaggt aggggtgctg acaaccgctc cagcttcaac aaaggtttca
     9001 atgtatccac gacgcaatgc ttccagatag atttcctggg tgccggggaa aataatcacc
     9061 cgcatttccg gatgaacctt gtgtcctttg agaatggctg ctgttgtttg cagatcttcg
     9121 attctgccgt tggtacaaga tccgatcacg acctgatcga tcaatacatc ggttgcttca
     9181 ctgaccggac gggtgttttc cggcaaatgt ggaaaagcca ccaaaggctc catttgggat
     9241 acatcgtatt ccttcacttc tgcgtaaact gcatccggat ctgattgata taacgtatag
     9301 ggtctttggg ccctgcccct gacataagcc aaagtgatgt catccggcgc aatgatgccg
     9361 tttttgccgc cggcttcgat agccatgttg gccatggtaa aacggttgtc aatagacagg
     9421 ttggaaattg cttcgccgca gaactccatt gacatatagc gggccccgtc cactccgatg
     9481 tcaccaatgg cccgcaggat aaaatccttg ccgccgaccc actggggctt tttcccataa
     9541 tagacaaact tgatgctttc cggcacttta aaccaggctt ctccggtcgc catggcagcg
     9601 gccatatccg ttgagccaac cccggtagca aatgccccca gcgcaccata cgtacaggta
     9661 tgcgaatcag cccctatgac caagtcccca ggtcccacca gtccttgttc cggcaaaaga
     9721 caatgttcga ttcccattgc gccgatttca aaataattct ttatgttaaa cccacgcgca
     9781 aaatcacgca ctgttttggc ttgttcggcc gactttatat ccttggcggg cataaaatga
     9841 tcgggaacca gtaccacccg ctcctgatca aatacttctt gcagaccgag ccggttaaac
     9901 tcagaaatag caaccggggc agtgacatcg ttgcccagga ccaggtccag tttacagttg
     9961 atcaactcgc ccggctcaag atattctctg cctgcatggg ctgctagaat cttctgactg
    10021 atggtcattc ccattagggc tcctcctttt tgcactaacg attaattttg ctgactgttt
    10081 tccagttgtt gttcataata aaatttgttg atggcatgaa tatatgattt gacactggcc
    10141 tcaatgatat ctgtactgag gcctctgcca ttgtaaattt tttcttcaat ctccaccttg
    10201 gagagtactt ctcccagcgc atctttgccg cctgtaacag catgcagctt atattccagt
    10261 agctttccat agatatcggt aatcttgtcg actgcatgac aagctgcttc catcggaccc
    10321 gaaccggtcg ctgcagattc aaaaggcatg ttatcaatac gcaatctgac cgtggcggta
    10381 gggatcatgt tggtgccact ggagatatgc agatattcca gactgaaacg ctccggaata
    10441 gagcgccctt gatccttgat caaagcctca atgtcctcgt tgctgatttc ctttttcttg
    10501 tcagccagat ccttgaattt ttcaaaggct cgctgtaatt cttcgtcgct cagatcatag
    10561 ccgagctcca tcatgcgtgc cttgaaagcg tgacgtccgg agtgtttgcc cagcaccaga
    10621 ttgtttttaa agacgcccac cagttcagga ctcatgatct cgtaggtcgt acgttctttg
    10681 agcacaccat cctgatggat gcccgattca tgtgagaaag cgttgctgcc cacaatcgcc
    10741 ttgttaggct gcaccaccat accggtcagg gtactcacca ggcggctggt gcgataaatt
    10801 tcctcatatt ttacattgac ctctttctga taaaacgcat gcttggtgta caaagccata
    10861 acaatctctt ccaaagaggc attgccggct cgttcgccga ttccattgac ggcgcactcg
    10921 atttgccgcg caccattgta cacgccggcc aacgaattgg caacggccag acccaggtca
    10981 tcgtgacaat gaacactaaa gatagcctga tcactatttg gaacgcgatc aataacagtt
    11041 ttcataaaat gcccaaactc atcagggacc gtatagccca ctgtatctgg caagtttacg
    11101 accttggcac ccgccgttat gactgcttcc accaactgta ccaaatagtc cagatcactg
    11161 cgggtcgcat cttccgctga aaactccaca tcagagcaat actgacgggc atatttcacc
    11221 gcctccacag cttgggccag tacttcttcc cggctcttct gcagtttgta ttgaagatga
    11281 atgtcggagc tggccaggaa agtatgtata cgcggatttt ccgcatactt cactgcttcc
    11341 caggtacgat cgatatcagc ccgggtagca cggcacaatc cggcaatgac cggccctttg
    11401 acgttgcggg caatcgtttt gaccgcctgg aaatcaccgg gggaggcaat gggaaatccg
    11461 gcttcgatga tgtccacccc cagacgggct agttgttggg cgatttccac cttttcttct
    11521 acattgaggc tgaccccggg agactgctcg ccatccctca gggtagtatc aaaaagatag
    11581 atcctgtttt tgtcactcat gattatcaaa acctctgctt gtatttgtga agggttactc
    11641 cctatttgtt ttctttgacc aaggcattgc cggactttaa ccaaggcatc atggcacgca
    11701 attcagcgcc taccttttca atcaaatgtt cccgaccctg tctcttaagt gcattgaact
    11761 gcggacggcc cacctggttt tccagcagcc attccttggc aaattttccg tcctggattt
    11821 ctgccagtgc ctcttgcatg gcataacgtg cttcttcgcc gataaccttg gggccggtaa
    11881 tgtaatctcc ccactccgct gtatcggata cggaatagcg catgttgctc atgccgccct
    11941 catacatcag gtcaacgatc agctttagtt catggaagca ttcaaaataa gcgatttccg
    12001 gctgatatcc tgcttccacc aaggtttcaa aaccggtttt cacaagttgg gtaaccccgc
    12061 cgcacagtac acattgttcg ccaaagagat cggtttcggt ttcttcctgg aaagttgttt
    12121 ccaatactcc ggcgcgggtt gccccgatgc ctttggcatg ggccaacgcc aactctttgg
    12181 cttgcccggt gtagttctgt tggatggcta tcagggcagg cactccggcg ccctttacat
    12241 actcactgcg caccagatga cctgggcttt taggcgcaat catgaagaca tcgacattgg
    12301 ccggaggaac gatttggcca aaatgaatgt tgaagccatg ggagaacacc agtgcttttc
    12361 cttccgtcaa atactgggca atttcttctc tatatacttt ggcctgggct tcatcgggaa
    12421 gaaggatctg gataatatca ccctgccggg cagcttcagc taccgtcatg ggctggatgc
    12481 cggcggcaat aacccgatcc cattctgcct gactggcttc attaaagggt ttgcgcagtc
    12541 caaccacaac gtttacaccg ctctcatgca ggttttgggc ctgtgcatgt ccttgcgagc
    12601 caaatcccat caccgctacg gtttttccct ttaaaagctc cagatttgca tccgcatcat
    12661 aatacatctt cgccatttcc aatatcctcc ttaaaattct ttatttatta ccgcgaacca
    12721 tggctacttt gccggtccgc accatttcaa tgatgccaaa aggtcgcagc gactcttcga
    12781 tcgcatcaat tttgttttca tccccggtta cttcaattat cagcgaattc ttgccgatat
    12841 ccacaattct ggcccggaaa atatcaacaa tctggacaat ttctgcgcga acggtattgt
    12901 ccgctctgac cttgaacatg gataattctc tttctaccgc tttttcctgg gtaatatcaa
    12961 tgattttgat gacttctacc aatttgtgaa gttgtttgtt gacctgttcg atgattctgt
    13021 cgtctccttc aaccacaatg gtcatgcggg agatatcagg tgattcggtc cgacccacgg
    13081 tcaaactgtc aatattgtat ccccgacggc gaaacaaagt agcaactttg gttaatacgc
    13141 ccggcctgtt ttcaacggtt accgacagag tatgtttagc catccacttc accccccagc
    13201 atattgttga gcgcttctcc gggaggtacc atgggatata cattggtatt tcgatccacc
    13261 cagaagtcta tgatgaccgg cccgtcagtg acctgcaagg ccttttgcag cgcttcgtcg
    13321 acctcttcta tacatttcac cctcatcccc actgcgccat atgcttccgc cagttttaca
    13381 aaatcaggct gacaggtcat atcagtatgg gagtagcgtt caccgtaaaa aagagtctgc
    13441 cactgacgta ccataccaag atattgatta ttgagaatgc agataatgac gggcagtttg
    13501 tgctgtacag ccgtgcccag ttcctggata ttcatttgta tgctgccatc tccggcaatg
    13561 tcaataaccg gcaagtcggg ccgggctacc ttggcaccta tggcagcggg aaatccaaac
    13621 cccatagttc caagcccgcc ggaagttaca aaactacggg gatggcggaa cttgtaataa
    13681 tgacttgccc acatttgatg ctggccgact tccgtggtga tgattgcttc tccattggtc
    13741 aaatcactaa ttttttcaat gatatgttgt ggcggaatgc ggccttcaac tgagcctgca
    13801 taggaaagcg ggttttcgtc tttccatctt tcaatggttt catgccattc ccgaagattt
    13861 cctgcaggaa cgaggcgcgg cagcaatccc tgtagaatct ctttgacatc acctacaata
    13921 ggaacatcaa cacaaacatt cttgccaatt tccgccgcat ctatgtccat atgaatcact
    13981 ttggcatgag gagcaaactc cgcgatttta ccggtaaccc gatcatcaaa ccgaacccca
    14041 atagcgagca agacatcgca ttccccgatg gcgtaattgg cataacgagt accatgcata
    14101 cccagcatac ctagatataa gtgatgataa ccggagaacg cacccatacc cattaacgtt
    14161 gttgtaaccg gtatctgcat tttctcggcc accgccagca gttcatcggc tgcattcgag
    14221 gcaacaacgc caccaccggc atatatcact ggtcttcggg cagaattgat taaatcagca
    14281 gcagtgagca cctgccccgt gttataacct ttcaaaacgc gatagccgcg aatgttcatt
    14341 tctttagccg gcccatcgaa ctctatttct ctttccattg tatccttggg caaatcgatc
    14401 aataccggtc cagggcggtt ggtattggca atgtaaaaag cttccttaac cacttgggca
    14461 acgtcctcgg tgcgttctac cagaaaactg tgtttggtga tgggcataac gatccctgtg
    14521 atatcagcct cctgaaaagc atcccgcccg atcatatggg ttggcacctg accggtaata
    14581 accaccatgg ggatggaatc catataagca ttggctatgc cggttaccag gttggttgcc
    14641 ccgggaccgg aagtcgcaat gcaaactccg gttttaccgg taactcttgc ataaccatcc
    14701 gcagcatggg ccgcgccttg ttcatgacgg accaatacgt gcttgagcgg tgagttgtac
    14761 agagcatcat aaatggttat ggcctgcccc cccggatagc caaacacaac ttcaactccc
    14821 tcggcttgca gacaacgcag taaaatatca gcacctctta acttcacatc tatcacctct
    14881 tacctataaa atagcttatt tgaatgttta tagttgaata tcttattttt cttggcttat
    14941 atttaatttt ttctcctgtg ttttaaaaaa caggaataat taataatagc gggctgattg
    15001 atggcccgct aaaatcgttt gttcctgacc ggaaagtttt attttacaaa tactgccccg
    15061 gtactggctg attgaaccat ctgcgcatag cgcttcatat atccagtttt gatttttaac
    15121 tccggttcct gccagcttgc ccgtctggct tccagaacat cgtcttccac caatagttcc
    15181 agctttagcg ccggaatatc tattcgaatc agatcccctt cctcaatcaa ggcaatgttt
    15241 ccaccggcag ccgcttcggg ggatacgtga ccaatcgatg ccccccgggt ggcaccggaa
    15301 aaacgtccat cggtaatcaa ggctacttct ttatccaacc ccatgccggc gatggtggaa
    15361 gtgggggtaa gcatctctct catccccgga cctccgcggg gtccttcgta acgaataaca
    15421 atgacatccc cggcctttat tttgttagca agaattgctt caaccgcttc ctcttccgat
    15481 tcaaataccc gggccggccc ctgatgttgc aacatttcgg gatctaccgc acccttcttg
    15541 acaaccgctc cttccggggc caaattccca aacaggatgg ataaaccgcc gctttcacta
    15601 tgaggcttgt ttacggaacg aataacagaa taatcaagca ccggagcagt ttttatgttg
    15661 tctcccacac tggctcccgt aaccgtcatg gcattacaat ggatcaaccc ttttttatcg
    15721 agttcgttaa acagcgcctg aacgcctcca gcccggtata aatcctccat atgatgatta
    15781 ccggcgggac tgatcttgca taaatgtgga gtaactgcac tgagctcatt aaatttctgt
    15841 aatgaaatcg gaaccccagc ttcattggcg atggccatca ggtggagcac ggaattggtt
    15901 gaacagccga tcgccatatc caaactcatg gcattgtgaa atgctgccgc attcatgatg
    15961 tcagccgggc gcaagtcttt ttccaccagt tccaatatct tcgcgccggc agtcttggcc
    16021 aggcgaatcc gttccgagta aaccgctggg atagtgccat tgcctggcag acccattccc
    16081 agagcctcgg tcaaacagtt catggagttg gcggtaaaca tgcccgaaca tgatccacag
    16141 ctggggcagg cattttcctc caatcgggac agttcttctg tattgatttt cccgctggca
    16201 gcagccccga ccccttcaaa cacctgggta acactgactt ttttctccgc catgttacct
    16261 gccagcatgg gcccgccgct aataaaaatg gaaggtatgt ttaaacgtgc ggccgccatc
    16321 aacattcccg gtacgacttt atcgcagttg gggataaaaa ccagaccatc aaaagcatgg
    16381 gcgatagcca taatttccac tgaatcggca atcaactcac ggctgcccag cgaatatttc
    16441 atgccgatat gattcatggc aattccgtca caaaccgcaa tcgtatggaa ctcaaaagga
    16501 acccctccag ccattctaat gccagctttg acggcgtcgg aaattttatc tagattaatg
    16561 tgtccgggga cgatttcgtt ccaggaattc acaacgccaa taaaggggcg cttcatttct
    16621 tcatctgtta ttccgagtgc tttaaacaaa gaccgatgag gcgctttttc caaaccttct
    16681 ttaacgattt tgcttttcat taaaaccatc ccttcgcaac cttcaatacc acagcgggtt
    16741 atttcagaat attctcaata tcttaaaaaa ggatataaaa aaacctctcg tcctattcag
    16801 ggacgagaga tattcccgcg gtaccaccct gtttgtcgca ggaacgcttt gtctgcgacc
    16861 acttggagcc aacctttgcg attggctaac ttggtaacgg actttttcgt ccggcatcac
    16921 ctactgggct tatcaggctt ttcagatcgc aactccggaa tgagcaagtc tgtaaacatt
    16981 gataccggtt ttcagctgcc ccggttctct gcaatcaatc cgtttttcag accattttcc
    17041 atcatcgccg aataattgat ttctattgag tatatttgaa agctttccta tatgtcaata
    17101 tatttttaag ttttttctca ttttctcaaa tcaattttac gcagatattc ctttaccccg
    17161 attcgataac tgaaaccgtt catctgccgt ttcttttcct ttagtttgat ttttttcagc
    17221 atggaccaca aaagtctcgt cccttcctca ctctgacatg cttccgaagg actcatcccc
    17281 cgcaagccat gcaaatgaga atgataccat ttgttgaggt acatttcctt cagggttgct
    17341 tccagatcag gatatcgttt cttgacttcc atatccagaa tgctgaacaa tccttcttct
    17401 ctgacttcca acccgtcaaa ctccaaagcc tcatacatat tttcctcaaa accctgacgg
    17461 atgtagttca tctccttttc tccgaaagca gaaacggtca gccaattgct gcccatcata
    17521 tattctgccc taaaattagc cggatcaaag ccatcatcca gttcataata aatcttgatg
    17581 tcatcatatg tttctatggt ttcggcgttg agcttttcca atctgtgcaa taccttggtt
    17641 cgatccttga caaagtacct gacggaaaca aagcgcatcc catcttcata aaccaaatca
    17701 ttttcatcac ccagcaggat atcttcaaaa ttgttgtact gtccgctcag aaaacgatag
    17761 gcatcagcca gcgtaatctc atattcgcct ctttggacca gaaaaggata tgacccctca
    17821 caaaagattt tatgaaactg gtcaaccact tcctgctcct gggagatgat tacaaattca
    17881 tccatattga tgatcaagtc agcgatcacc ccggtcatga aagagacccc atggttaaaa
    17941 tcgtcttcat aacaagtaag ccgcatatga atcccataag ggagttcttc cacttcacgt
    18001 atattttcca cccggtccag gacatcgaaa agctcatcca tttccgtgat ttcataaacc
    18061 cggcaaaaaa cctgcaatgc aaaaggattc caaaccgatt cagggtcgat cagctgatgg
    18121 ctgacctgtt tgatatctat gatggaccaa tagccttgtg gatctctttt taacgcaatc
    18181 ttgtattcac tcttgtaaac ttcccgctct tgaagaatga ccgaaaaagc acgtaggtga
    18241 gcatgctcac cggatgccct ggcttcttcg acttgtcctt ccatgataaa agcgccggga
    18301 tttatggctt ctttaaattg atagaatagt tttccggaaa tcagctgcgt atccagaatc
    18361 gacagcagat aattataatc cttgtagcgc aaagcatgaa aaagactgtt gccaagttga
    18421 cggggagcca agcgggccga aatccttttg ttcaaatttt ggacctggtc cgctgtcaac
    18481 tccagcggtt cgtccaaata acgctggtaa ataaacttgc ccaaagcagg acggctcata
    18541 tagcgaacat ttaatttgta ggcctcggct atcaaataac aggcttccga ataatcaatt
    18601 tcagttatgt cggccatcaa tccatgatcc ttttcatact gcgattgggc gatatcctcg
    18661 accacaaaca ggctgtagat cccgtccgga ttgaaagtca ggaaaaagca ttccacgtat
    18721 aggtttccct gatccttgta ccaggctaca tccaaactga tacgcccggt atgacggcta
    18781 cagcttacat aggctttata gaatgaatag tcaaaggttg cttcatccgc ggttttaatt
    18841 ccggccagcc ggtatttgtt taagatgcgc tggcagagaa ccccatattc caaaccgtat
    18901 tcttcattta tcaattggaa atagctggcc atctgcgggc tgtacaagct tcccaaacct
    18961 tccaggaaat tttcccgtaa atccagtggg ttttcttcaa atatttccaa caacaggagt
    19021 ttgccccagt tgctgtcgat ataattgttt atttcttcgg ctctttgctg caacacttgt
    19081 ttagtataat agccgttgtt gtttttcatg cttttcccct aatctattaa taatctcaat
    19141 ccatatgtaa tgtaccattt tggcgagggg atagcaaatc ttgcactttg attgattttg
    19201 ggcagattgc cgtaaaaaat agaaatcggg tagaatatac gcataataaa aaaactatta
    19261 tgaggtgaga gaaatgttat gggaaatgcc cggttctgat aatacccgag aaacgctgca
    19321 attggcagtc aaacgaagca acgagttaag catcactcat ttcgtcgtgg cttcctgcag
    19381 cggcgcaaca gcccgggagt tgtatgcgct ggttcaggaa aaaaagatgg tctgtgtaag
    19441 tcatgtggtc ggctttgcgg aaccaggtgt ggatgaaatg ttgcccgaaa accgcgcatt
    19501 ttttaatgcc cacggcgttc cggtgctgac taccgcacat cttctggccg gtattgaccg
    19561 ggccttgcgc tatcagttta aaggagtcta tccagccgaa atcgtggctt caacgctgag
    19621 aatgtttggc caggggatta aagttgccgt ggaaaccagc tgtatggcac tggatgcagg
    19681 gatgattcct tacgggcagg atgtgatcgc aatcggggga acagttgccg gcgcagatgc
    19741 cgcgatcgtg gtaagacctg cccattcgac gcattttttt gccacgcaaa tcaaagaaat
    19801 catatgtatg ccgaaaaata aaaaataaat tgtaatcgaa tgatattatt gacatagttt
    19861 atgctatttt gctgctttgt aaaatttaac cggccataaa ccaaacagat tttgcaaaag
    19921 ctatctctat aaaggaggga tatattttgc agagaaaatc atccatgaaa ttcgaaaatc
    19981 taatggtacc catggccttt gtcaacggtt tgtttttggg tggttacgcc ctgcgcatgg
    20041 ccagaaaata ttgggaagac ggtccgcagg attaaccttc cgtgaacaaa catcgggctc
    20101 acgaaaagtg agcccaattt gttggtatta ttatattata caaagttcac agtttgttta
    20161 ttgcagccgc aatacttgtt tttcctgcag cgaatccatt tgattcgctt taatcatcgc
    20221 cggcgacaaa gtatagagcg catctccaat gtataaaata cggttaatgc ttttatcagg
    20281 attgtcccag taatttcccg ctttcaaata atcatcctgg tcgaggtggg taatcctgcc
    20341 tttgaactgc agcccttgct gcaagtctat atgatataca taggcacctt ggaaggaaaa
    20401 tcttccatat tcaggaatat tggcattatt ctgttttgca ttcttttgtc caatctccat
    20461 gaccgtcacc ggcagtgcca gtaaattctt ttctcgcgag aacagcaatg ccttgtgatt
    20521 gcgcaaaagc tccgaatcac tgccccggtc gccaattact actttgctca tttccaccgg
    20581 ctgattgaca tcagttacat caaaaacagc aattttcaat ccttgataaa aagccatact
    20641 cttctcattg tagcctgaca attcgatggt ttccttgccg aatccaatga tatggttttc
    20701 gtcataggga tgcagatagt cactatagcc gggaattttt aatttgccca gtattttggg
    20761 ctgggccgga tcagtcagat ccaaaacaaa aaatgggtcg acgtctttaa aagttaccat
    20821 gtaggctcga tggcccataa aacgagtgga gtaaattctt tccccgggtg caatgttttc
    20881 aatttgaccg atgagtttca aatcgcggtc aagcatatat acattgttct gtgatgtgta
    20941 ttggtcatcg cgccacacat ctccataagt ggtggcaatg cgaaaggtat cgttgtattc
    21001 gtccatgcta aactggttca atatggtgcc cggcacctgc ccttctcctg catactgcat
    21061 ttgggcgtcc ttcatcttaa atttgaaaat tttcgttgtt gtatctgccg gacttgcagg
    21121 cgctgtactc aaggtggaac ggataacatt attgaaatat tgactggctg ctacataaag
    21181 gttttgggtc gaagcgtaaa cgttttcacc gccacccagg taggtattga ttgcggcagg
    21241 tttatccatc tgttgcaaat cgattgcacc ggcgaccaaa taattggggg ttatgcagtc
    21301 cggaaaatag cgaatcgtct tgatatcctg tacatcaaac cgatcggctt tgatagtatc
    21361 cttgcacata ggggtcgttt ccagaccttt ttgtatggaa tagtaatcca catgacaatt
    21421 gctgagcaga tatagttttg aatctacgag acgcgaggtc agataactgc cttccagaac
    21481 cagtctccgg atttggctga tatttcgttt atccttcata tcatagacaa tcgttcggca
    21541 taaagatgcg ttacggtgat aatagggcgg catggcagct gacttcatgc gcagctggga
    21601 ttgatcaacc ggatagacag gctccggtat ataagtattt cctattacaa tcaaataatc
    21661 tccgtcaata tatatttcaa ccggactaaa attttggtca ttaaatctga ttgtattgat
    21721 gatttccatt tgttcaaccg gaacagcttt gataatttgc acctggttat ggttgatttg
    21781 ataaatatat tgcccgtctg tttttacctg atcgccctcg tccactcctt caacctgtac
    21841 gttggtagct gagtaatcat ctgcctgacc tgctgcaggc atggcggctt gacttttcat
    21901 attggtcgga atgctttcgt ccaccgccat ctcccggtac atataagcgt ttttttgttc
    21961 atattgttcc aacaaaatgc gcaggttttc ccaggaaccc accactggca attccttgtc
    22021 tgctcgagca accgcaaccg ggttctgttt ttccggaaac aaagcggtcc ccgccagcac
    22081 ccccagcata atcaacagca acacaaacag aaatgatgcc attttggtct tgtttttagc
    22141 catccctgac cctccccatc gtttcgtttt ctataaaact caatacgcag aagaagtcgc
    22201 tggcctttca tgggagctga ccaatgccat caggatcctt ccgaaaagct ggcctgcacc
    22261 acaggagcaa acaccatctg cgccactttt tgtccgggta ggatgcgcag cggcttgtca
    22321 gtaatattca gcagcagtat tttcacttct ccggtgtgct cacagtcaat cgtaccggga
    22381 gcattggcaa ctacaatccc cttgccggcc atgcccgccc ggctgcgaat ctgagcctca
    22441 tatcctttgg ggatcatcat ggcaaaccca gtaggaaaac ttcgaacctc attgggataa
    22501 aggataattt cttcctgaat ggcaatggcc aaatcacagc ctgctgcccc ccgtgtctca
    22561 tattcaggca atttcgccca ggataccagt tttttgaatt tcacttcgat cttgtccata
    22621 ttactacctc ctactgctat gatgaatcat ctgattctat tctagcgtat ctcgttaaaa
    22681 agtttttacc tttcttcatc catttaccat gaaattctga taattattat tttattccat
    22741 tcatcaacgg tttgccgggt tggtatgatt ggcccggcaa aagtccttgc cgcgttcata
    22801 acctaaaaat atcagcttcc attacagaag ctgactgaaa tgctgtaact gagtctcaag
    22861 catcgattgt ctggagcagt tcgtccaggg tgacaaattg ataaccttgc ttttggtagg
    22921 cgtcaatgat gatgggcagg gccaacaggg tttggcggcg atccaatctg ccgtcatgtg
    22981 ccagaatgat catccctgga cgggctgcct caatgactct tttagccatc gctgcaggcg
    23041 ttggcgaacg ggcattttcc acgcagatgg tccataaaac ggtttgataa ccgttacgct
    23101 cggcgatttc aaccgtttcc ggccgaaaca gctttttggg cggccgaaaa tacttgcttt
    23161 tcctgcccag cataactgac agcagctgat cggttttttg gatttccgct tctgtccctg
    23221 cagtatcagc cagactcaaa tcagcatggg tataggtatg gttctcaatt tcatgacccg
    23281 cctcaatttc ctgctgaatc agctcaggat gggcagcagc gttttcacct acgacaaaaa
    23341 aggttgcctg cacgtcgaat ttttgcagga tttccagcac tgcaccggta tacaaaggat
    23401 ctggtccgtc atcaaaggtg agagccagcc gtttttttcc gccggtgtcg gcgtgagtga
    23461 tcagtctttt attaagattg ctgtccacca cataaataac ccgcgacatt tccgaatctt
    23521 cctgggtaaa tgtctgtatt gcatacaact gggatttcaa caaacccagc aatagaatcg
    23581 cggccatcaa tattcctatg gttttaacta catatttcaa tgtttattct tgccccctga
    23641 ctgactggca agtgcttgtc gtattcaatc tgtttatggt gagcttagct tttacataaa
    23701 tgtgccgcct taaacatccg gttgaagatg ttgacccact ctttgaaaag cgatgataaa
    23761 actatataat gataaaaatg ttgcaacaaa aggagtttcg ccatgcaaaa tccagccata
    23821 cctgcccggc ttgcagggat cacttctttc attgtcatgg atgtattgga gaaagcccaa
    23881 tccatggaag cgcaaggaat agatgtgatc catctggaaa tcggtgaacc tgatttttct
    23941 actccggaag cagtgaagga tgcggcgttg aaagcaattc gtgaggacga tacacactat
    24001 acccacagcc tgggcaaatt atccctgcgg gaagaaattg ccggttatta ctggcgcaaa
    24061 tacggggtga aaatttcacc ggaacaggtc atgatcactt ccggtacatc ccccgccatg
    24121 ttaatgattt tttctgtttt gctggaaggc ggcaaagaaa tcatcatgcc tgatccatac
    24181 tattcgtgct atcccaactt tgtcagatta acagacggta aatcggtatt ggtaccagtc
    24241 tatgaagaag acggtttcat ataccgtcag gaaacagtcc gtcaattgat tcgtccgtct
    24301 acccgtgcca tcttggtcaa ttccccctcc aatcccaccg gggtcgtata cccttcggca
    24361 atgctgcaat ctctggccga tacggggcag atgattgtat ccgatgaaat ctaccacggt
    24421 ctggtctatg ccggccagga acactcgata cttgagtaca cagaccgtgc ttttgttatc
    24481 aatggctttt ccaaacttta tgccatgact ggctggcgtc ttggttactt gattgctccg
    24541 cctgcctata tgcgaacatt acagaaattg cagcagaatc tctttatttg cgcctcctct
    24601 tttgcccagg aagctgccat tatcgcactg cggaactgtg aacaggtagt agagaaaagg
    24661 cgcctcattt atgatgaaag acgccaatac ctgctgactc gtttaagaaa aatggggatt
    24721 gctcccgcag tcgaacctac cggggctttc tataccctgg ccaacgtaaa aaaatatacc
    24781 cgtgattcct accaatttgc ttttgaaatc ctggaaaaag cacatctggc ggtgaccccc
    24841 gggattgatt tcggagccaa ttgcgaaggt tatatccgta tctcttatgc caattctctg
    24901 gaaaatatac aggaaggaat ggaccgtttg gaaagttttt tggccggttg cacgctcctc
    24961 tctgccgagt tttaaatgcc ttccgccccc cttgttcagc caaaaaagcc cgcttgagcg
    25021 ggctttttga ttcatttggc taatattccc tgggcacaaa tgtttcgcaa caggtgccgt
    25081 cgctgctgtt agccagtcca tcccccagcg catttacttc aatccctttg gcattgcatt
    25141 gataattctc attgtacgca cagttggcaa cggaacactt gattttctgc acctttnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnataa ttctcattgt acgcacagtt
    25321 ggcaacggaa cacttgattt tctgcacctt tccacccctt tccagtaaaa cacagtatct
    25381 atcgtcagct accattaatt ttcccggctg agccaaagat atgtattcaa ctgcaagtgg
    25441 ttcatttttt tcaaaggcat tggctgaact caatgttgaa aagtatatac agcacgttga
    25501 ttaagcgggc ttgggcgaat aacgcagcga ttcactcttg taatcggtcc ggggtgagct
    25561 atgtacgacc caggtatcac tcagcatatc atgcagacat tgcttatccc ggttaaacca
    25621 gcagaagata tatcccagat acaagcccag catttgcgaa aggcttctgg ccaccagttc
    25681 ccggaaaaca acggcttgcc atgtcaaggg ttggtcaccg gcctgaatga cttttatgcc
    25741 ggcgatcatc ttgcccagtg tttgctgaaa ataacgcgtc ataatgataa aataggctgc
    25801 gccggtgatt cctagaaaca ggtcattgag acgaataaat ttgtagacgg aagtatgctc
    25861 ggtctgcgca ggccccaaaa tatgaaacag aatgccattc agacaggcaa ttactcccaa
    25921 atcaatgaaa aaggccaggg ttctgatcca aaatccagcc actcgatagt attgctcggg
    25981 ctcatgatct aacgtagttg ttgttggatt gttttcattg gcattaaggt tttgttcatc
    26041 catgttgctg ctcctttatc aatgagataa atacatgatc tgcggagcgc tttgttgctg
    26101 aagctgctcg ctcaactcct ttaaatcaaa ggcgggggcc cgcactgaca tgatccagtt
    26161 caaaaaatca ggaccgggat tttcatataa aaggacctga gggtcatttg ttttggttat
    26221 cttggctgtt ccttcaatgg catcctgcaa ataacccaac tcatcgatca gccccaattg
    26281 cttggcctgc gctcctgaat aaatcctgcc gtctgccaat tttagaacct cttcgcgatt
    26341 catctggcgc ccctcaacaa cgatgtccac aaagtagtta tagctttcat cgacaatccc
    26401 ctgcaaaatc gtctgttctt cagtcgtaat cggtcgcagg ggattcaaca aatccttgtt
    26461 ggcagcactt ttaaagctaa catcctgcac ccctattttt ttcgccaatt cactaaaatt
    26521 atacgtgctg ataatcacac cgatcgatcc ggtcaaagtg ttgcggttgg cgtatatctt
    26581 gtcggccgcc atggcaatat aaaaagcacc ggaagcagcc tgctggctca tgtagacgac
    26641 cagcggcttt ttatattggg ctttcaattc catgatacgg tggtacactt catcactttc
    26701 atagactccg ccgccgggtg aattcaattg caggatgatc cctttgatag aagggtcggc
    26761 ataagccgct tcaagctgtt tgagaaaata acgatggttg tatgtgctgg tcaagcccat
    26821 actgctgtcg ttattctcca tgatgacccc ttccagtttc aacaaggcca gcttgccccc
    26881 gctgccttcc tgatagacgg ttgttttcca gttgccttca tttcctccga tgtacctggt
    26941 aagagcactt gtcgcctgct tttcttcctc gatacccgca aatgacccgc tcatatatac
    27001 aaagatcaat accagaaata tgcccagtgc ccaccatcgt tttttattca ctgccttttg
    27061 cctccttcag ctttacgata ttcgctgtat attttatttt atctctttta aaagctatat
    27121 gtctcatttt taggattttg ctggaaaata gttgccccgg ttattcattc tctttgttat
    27181 cctccccggc ttccaaaatg tccatgtctg atagattttg caggtcgctt tgagggatgt
    27241 cctgcacttg tcgtaatttt ttttcgatgg tacgtgattt gcgggatgcg gtctccagac
    27301 tgtggctggc ctctgtcaat tttttatggg ttttctccag catgtcgcca aacttgccaa
    27361 attcggtttt tactgctccc agcaaggccc atacttcact ggaacgtttt tcgatcgcca
    27421 aggtatgaaa gccaattgcc aagctgttga gcaaggcggc catggtactt ggtccggtaa
    27481 caatgacccg ggtatctctt tgcagccctt ccaacagatt cggtcttttt accacctcgg
    27541 cataaagact ctcggtaggc agaaacatga tcccaaaatc agtggtatga ggaggactga
    27601 gatacttgtc atgaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcag
    27721 ccagggtctg catctcgccc aatcctttat gtaccatctc caaacgttca ctgacttgtc
    27781 tgaaggattc tcccaaacgc tgctccaggg ttttgtgcag cttttcatcc accgtagccc
    27841 gcatttgttc cagctgtctg cggttgtctt cctgcaaggt tttaagcttg tcttccacgg
    27901 tttgtcggac cgcctccagg cgagtatcat tattttgggt aatcgttccc agctgacggg
    27961 caaaaagttc cagctgatcc ttctgtagtc cagcgatttc agtcattcgg gccaggacaa
    28021 cattctgaaa aacggccagc gattgggctg attcttcccg gttgtcgcgg gcattccggc
    28081 tgttttcttg acgtaattgt accatctcgc tgcgcagtac cccgcccagg ctttccagat
    28141 tcttttccag catctcctgc tgctgcagcc attgagaaac gcggttgctg atatcctgtt
    28201 gctccaactt ggcctttagc cggtattgat tgaatataat gaaagctatt aataatatgg
    28261 cgactgcact gattattaag taatccttct ccattctttt gaacctccgg tattctttct
    28321 tcccgaatta tccatagtga acaaagagcc atgaatagcc ccttgtttcc aaattactac
    28381 aaccccgacc cggtgtaaat ctttttcctg ccgaaaaaca aaggttgtct tacgatgatt
    28441 aaaactgttt tacctggttg gaatggttta taatttggat tgtaaaatct aaccatgctt
    28501 gcaggcattt aatttttgcg agtatgaaaa gaaggttggg caatgttata ttctgtaggt
    28561 aaggatattt atctcgtggc tccccgcgtt ccccgttatc catacagcaa cagcctctat
    28621 attgaagacg aacggcccat ggtgatcgat ttcggtgccg gcggaactgc gtttgtcgat
    28681 gttccccacg aaaaaatcga attgggtctg atcagtcact ttcattttga tcatatccac
    28741 agccgcagct tgtttccgaa catcgctctg atggccggtg cagaggaaaa atttacttat
    28801 gtcgatcaaa atgcctatat ccattttcat ggttatgacc tgtgggatga gcttatgcat
    28861 attgagcggg aaatgtatgg tgatgtcatt aaaattcccc gggacattcc catcgaaccc
    28921 ggctttaaaa cgattcatct ggcagataca ttctccgacg gagatgtaat ccaaactggc
    28981 aaatacgaag tgatgaccgt tcatcttcca ggtcacagcg ccgggcatta tggtttctat
    29041 ttggaaaaag aaaacatcct cttctccgct gatattgatc tggtcaaaac cggaccttgg
    29101 tataacagca acagcggcag tgtaggcgat ttgattgctt ccgtacaaaa aatcaaggat
    29161 ttgtccccgc gtatggtcat cccttcccac cgccgtatcc agacggagaa tctggtttct
    29221 cagttggatg cttacatcca ggtggttttg gaccgggaag agaaaatata taactatctc
    29281 caccaacctc ataccattga ccaattggcc acttatcgcc tcacttaccc ccagcagcgg
    29341 gaccaatatg aagcattctg ggaaaagatg accatccgca atcacctgcg ccatatgctg
    29401 gaactgggct tgatcaagga aatcgagccg ggagtatacc aacaaatata attcttttct
    29461 tttttattgt atacaaagaa attacattta tttgcaggat attaatattt gacgtcgaag
    29521 aatatcggtt atggaaataa ccggatggaa acaatggggg gttaaatatt aatcatgggt
    29581 aattgggagc agaatgtagc tgctctgctt gccaatgaga aaacatcctg caaatccggt
    29641 aaaagatgag gagggaatta attggaagct ttttcggcgt tgatcagtac cgtcaatgga
    29701 ttcgtgtggg gtccctggat gttggtttta ttggtgggga caggcatatt tctcacgtta
    29761 cgtctgggtt ttttacagat tacccagttg ccttatgctt taaaactcgc attctcacca
    29821 tcccagcagg ataaaatctc caaaggcgat atttcgcatt ttcaggcact catgaccgct
    29881 ttggcagcaa ccattggtac aggtaacatt gttggtgttg caacagcggt tgttttaggt
    29941 ggtccgggag ctgtcttttg gatgtggata acagctttgt tcggcatggc taccaagtat
    30001 ggcgaagctg tcctggccat caaattccgg gtgacggatg aaaaaggcga aatggccggc
    30061 ggcccgatgt actatattga aaagggttta ggctggaaat ggctggcggt gctgtttgcc
    30121 atttttggtt ctgtagcagc ctttggcatc ggcaatctgg tgcaggccaa tgcagtagcc
    30181 ggcaatatgc atgatacctt tggcatcagc ccggttatca ccggccttat cctggctgtg
    30241 tttaccgctt tggtgattct cggcggagta aaaagtattg gccgctggac cggtatcatc
    30301 gtacctttta tggccgtttt ttatgtttta ggcggactct atatcatctt tgccaatttc
    30361 catatggttg gcccggcttt tgctattatt ttcgaatcgg ccttttccgc cgaagccggt
    30421 ttcggcgcat tggttggcac gaccattcgt tttggggtag cccgcggtgt attttccaat
    30481 gaagccggtc tgggttctgc tccaattgcg gcggctgcgg ccaagaccga tcatccggga
    30541 cggcagggac tggtatccat gaccggcact ttcctggata cgatcgttgt ttgtacgatc
    30601 acaggccttt cactggtgat ggccaatttg tatatcggcg tggaaaaagc cggggcggct
    30661 gcgctgacca gtcaatcgtt ccaacatttt atcccctttg gaaacttcat tgtgacgatc
    30721 gcgttgttat tttttgccta ttccaccgta ctgggttggt cttactatgg tgaaaaatgt
    30781 ttctattatc tggtgggaca aaagggcatc gttccctacc gtgttttctt ctgcgtattg
    30841 gtctttattg gggccatcct gaagttgggc ctggtttggg atatcgccga tacctttaat
    30901 ggcgccatgg ccatccccaa cctcattgca ctgttggcac tgtccggcgt aattgctgct
    30961 gaaaccaagg aatttaaaca gatacgcatg aaagaaaaag cggaagcacg agcccaaaaa
    31021 atggccggtt aaaattgatt gcttgacaat ttttcgagca tgtggttata tctttttata
    31081 accgttgaag gggaagtaaa accacagccc agcctcaaag agagtcggaa gcggtgtgat
    31141 cccggcagga gatggagtgg acaaatgggc cccggagggc ggggtgaaaa ggcgccagcc
    31201 tgagtagctt ccgacgcaga gccagcgtta cagggcgaag ggtgtgttgg cactctgtat
    31261 aagcggatac atgatccatg tatcaattaa ggtggtaccg caggtttttg cacctgtcct
    31321 taaccgggac aggtgctttt tgttgtttct attgcagtac accggatcct tggtttaacc
    31381 cgcggatgaa catgaggatc atacagggaa gggcacacgg acgattttat ttaatataag
    31441 gaggatacga aatgaaaaag tctattgtgt tgttgggttc attattggta ctgc
//
LOCUS       DAOTHZ010000018        31044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1644_length_31044_cov_7.530704, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000018 DAOTHZ010000000
VERSION     DAOTHZ010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 31044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31044
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_31044_cov_7.530704"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..228)
                     /locus_tag="P5549_03700"
     tRNA            complement(152..228)
                     /locus_tag="P5549_03700"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:His,seq:gtg)
     gene            complement(252..328)
                     /locus_tag="P5549_03705"
     tRNA            complement(252..328)
                     /locus_tag="P5549_03705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(292..294),aa:Arg,seq:tct)
     gene            complement(336..409)
                     /locus_tag="P5549_03710"
     tRNA            complement(336..409)
                     /locus_tag="P5549_03710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Gly,seq:tcc)
     gene            918..1721
                     /locus_tag="P5549_03715"
     CDS             918..1721
                     /locus_tag="P5549_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRW12211.1"
                     /translation="MRCDYHIHSSISADSSTRPELQVKQAEALGLEEICFTEHLEIHF
                     YRGDAWHVNVAEYREQFRRLASDRVKLKFGVEAGIALASEHFPELEGELRAADFDFVL
                     ASAHSIDNIDPFDPRFFEGKTLEQVFRNYIASILAGLKQLNTELFSSVGHIDFPSKGA
                     HSEEDARLFYRYAADEIDTLFRYLIAQGKCIEINTASYRQLNGLAIPGEDWLRRYAQL
                     GGEYVTLGSDAHSPEFIAYRLEEATEMAKRAGIKYYATYEQMVPVFHKL"
     gene            1803..2768
                     /locus_tag="P5549_03720"
     CDS             1803..2768
                     /locus_tag="P5549_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HRW12212.1"
                     /translation="MKGEVYNQSTWIALKAASYYYEHNLSLKEIARLLNVSEATVSRL
                     IRRARKEQIVEFVIRDPYKQCLDLEHRLQDGFGLKAVIVTAPAARQDPEEDKKQVALE
                     GARYLQRIITAHDVLGIAWGGTMYYLVHYLNPCQRTDTIFVTLHGSLSCCDYELDVHN
                     LVSRMSMALGGNYYSLSAAGRFDEVESLQAIRHEPNVKQVFSLFDQLTISVSGLGSFY
                     PELDSPLSRLQYLQPAELADLQAKGVYGDMLLRFFNRDGQECDSDVRQRTLAIDYATY
                     KRIPCKIAVVSGAQKAQSLAAALRGNLIDVLVVDHHLAQAVLTLP"
     gene            3161..4477
                     /locus_tag="P5549_03725"
     CDS             3161..4477
                     /locus_tag="P5549_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HRW12213.1"
                     /translation="MYIDPIICQFPEALDEERFIIATYYCAAKPSTNMLKFAAALAVE
                     QTTGTWLKVPAETPEVRERCIGRVVGVYETPAYQIEIPADVQERHFIIRIAFPWANFG
                     AQFAMMLSSVIGNISSSGKVKLIDLEFPKAFLAEFQGPKFGVQGIRELLGVYDRPLLN
                     NMIKPCTGLDAATTAELAYESAIGGVDIIKDDELVADAPHCRLLDRVKAVTAAVKRAD
                     EEKGEKTLFAFNITDRTDKLKDNAYRAMEAGANCLMVNYFTIGLDATRMLTEDPNINV
                     PILAHSDFTGAVYESPWSGMSASLIGAKLPRLAGLDMVIGLSPYGKFPIMMDTFINLG
                     YNMLAPLHNIKPVFPMPGGGTTQGHVEDIMKKFGNDVIIASGGAIHGHPMGPAAGARA
                     FRQAIDSVMAGMSLEEAGQKYTELGVALNAWGIYSEAQGGIFDLKG"
     gene            4517..5359
                     /locus_tag="P5549_03730"
     CDS             4517..5359
                     /locus_tag="P5549_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRW12214.1"
                     /translation="MAKQYTREQVLERVRKSVEAGKPVVIAGAGTGISGKFAERGGAD
                     LIGVYNSGLYRMDGNGSLAGLMPYGNANQIVIDLAHRVFPQIQEAPMIAGICGSDPTR
                     EMKPYLKYLLDLGFSAVMNFPTVGLIDGRFRQELEDTGMGYGKELATLKLAAELGMFT
                     IAYAFNEAEAEMVGQAGMDVMICHMGLTTGGAIGSKFSEKVTLEDAAALVNRMSAAAR
                     KGNPNVIIFAHGGPISSPQDTAYIYQHTDSVGFLGASSIERIPIEVPLTEAVKDFKNQ
                     SLKK"
     gene            5385..6722
                     /locus_tag="P5549_03735"
     CDS             5385..6722
                     /locus_tag="P5549_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HRW12215.1"
                     /translation="MTAKILVAGILDTKGEEIKYIARQVAAAGAKPTILELSVGKEVG
                     WADIGLSEILKEIGKVPEELYKVDRHQASAWVTEAAIQYVGRMVSQGEVDGIIACGGS
                     MGASIATAIMQSMPIGIPKLMATTMTSGDVRPYVGTKDICMMYPIAEAGINTLTKRIL
                     TNAAGAIVGMANSPAIDTSDDKPMIGCMMFGVTTPCVLRASKTMEDHGYEVMINHAIG
                     SGGLSMEQLIEDGFIEGIMDITTHEIADEMLGGVLSAGPDRLTAASRKGIPQVVAPGG
                     LDLINFGPLDTIPKHYMEQTNLPGRSLYEHNPAVNCIGVLPDEAYKIAENMAIKLNKS
                     QGPTVICVPMRGWGACDLGAPNKELGWAGPAAGPSWVADPDKAEWSLRSRRFMDGMKE
                     FLDLSKPNIELLEVDKHMNEPEFADLMSGLLKDMLDGNWKKNSRSHLSYVHSI"
     gene            6737..7570
                     /locus_tag="P5549_03740"
     CDS             6737..7570
                     /locus_tag="P5549_03740"
                     /inference="COORDINATES: protein motif:HMM:NF020927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HRW12216.1"
                     /translation="MAQKFSRQQILERLHGQIKAGRPLLMFGAGTGLTAKCAEKGGAD
                     LIGVYSTAIYRMRGLPSLLAWMPYSNANEHLLNMAGEILPIVQTTPCIAGIGAHDPAL
                     DLDAFLDTVVQAGFSGVTNEPFVGLYGKYFADQLERAGLGFSREVELIRRARAKNIFT
                     VAWAMSPEEAYQMAQAGADVIGAMIGVTSGGMSGASDVISLEEAVQDVRDMVAAARQA
                     DPQALLLTHGGPFADVATAQYSIQHTDAVGYASGSSGERIPTEEAVIGITLQYKAMQL
                     E"
     gene            complement(7702..8781)
                     /locus_tag="P5549_03745"
     CDS             complement(7702..8781)
                     /locus_tag="P5549_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRW12217.1"
                     /translation="MIQKIGIIGLGVIGGSLGMALRSNGLGVIVAGRDLDESTVAQAI
                     ELGAIDQMLDDRFLAQCDLVILAAPLTAMPAVMEQIKAHVKPGAIISDAGSVKGWVMQ
                     QLEAHFPADVFIVGGHPMAGSEKSGLQAADKFLLENAAYILTPRTGTPEAVIAQLQEL
                     LTAIGARVVIMNAAEHDQAVARVSHLPHLMSTALINNLLTQPEFMNLAGGGLRDSTRT
                     AASDPDLWEDILFLNADAIGQELAAIQQLLSIYRHSLQRKGDGVLRTYLQSASTTRKN
                     LPGLRPCLAEACDVIAIIPDQPGVIGTVGTLLGQANINIQNLQLLNVRDEDEGSIKIT
                     VPRESAPGACQILKQHGFQSWLLEK"
     gene            complement(8778..9815)
                     /gene="aroF"
                     /locus_tag="P5549_03750"
     CDS             complement(8778..9815)
                     /gene="aroF"
                     /locus_tag="P5549_03750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRW12218.1"
                     /translation="MVVVMQKGVSQEQIAKVQKHLEAEGYSVHLSQGAKRTIMGVIGE
                     PGQIDPQTLASLPGVDKVVPIMQPFKLASRDFKHQNTVVQVGPQLIGDSHVTLIAGPC
                     AIEGRENFLEAAAAVKDAGAQMLRGGAFKPRTSPYSFQGLGEKGLQIMAEARELTGLP
                     LVSEVMDQKSIPMLAEYVDMIQVGARNMQNFFLLRELGKVSKPILLKRGMSATIEEWL
                     MAAEYILGSGNPNVILCERGIRTFETYTRNTLDLSAVPLLKQLTHLPVIVDPSHGTGK
                     RELVEPMSLASVAAGADGLIIEVHPDPAQAWSDGPQSLFPEDFARLSSKVRRLESLTA
                     PATDPAAGFGQ"
     gene            10120..11418
                     /gene="aroA"
                     /locus_tag="P5549_03755"
     CDS             10120..11418
                     /gene="aroA"
                     /locus_tag="P5549_03755"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRW12219.1"
                     /translation="MDLTVKPSQPFRAVLRVPGDKSVSHRSVMIAAIANGVSTIDNFL
                     PGDDCLSTVRCMRQLGVKIEELTPTRLQVHGCGLHGLQEAGDYLDVGNSGTTIRFLSG
                     LLAGQPFFSVLTGDASIRRRPMGRVVKPLRQMGAQIWGRDGDRLAPLAIRGSHLQGIH
                     FDSPVASAQVKSAVLLAGLYARGETSVTEPAQSRDHTERMLAGFGADIKTEGLTTVIR
                     SGELAGQPVLVPGDISSAAFFLAAAAIIPGARITVQDLGLNPTRTGIIEVLQAMGATV
                     VIDNRRNVSGEELGDVSVTGGALKGLEIGGEIIPRLIDEIPVLAVVAAVAEGQTVIRD
                     AQELKVKESNRLQAVATELRRFGADIQETADGLVIQGGRQSYHGAVCESYHDHRIAMA
                     SALMGLVSKGETTVHDAECINISFPGFAHLLAELGGGEGC"
     gene            11412..12530
                     /gene="aroC"
                     /locus_tag="P5549_03760"
     CDS             11412..12530
                     /gene="aroC"
                     /locus_tag="P5549_03760"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRW12220.1"
                     /translation="MLRYLTAGESHGPALTAVVEGIPAGLELTADFINRQLARRQKGY
                     GRGGRMKIETDQADILSGVRGGKTLGSPVTLQIKNHDWQNWSQIMASGEADLQSRRVS
                     VPRPGHGDLAGHIKYGHDDLRNILERASARETAARVAACTVGRRLIEHFGMEIHSHVI
                     QIGSIKAQPASLQEIIDRAPQSELFCADPEAEALMKQEIDQAREQGDSLGGIFEIIVC
                     HVPVGLGSHVHWDRKLDGRLAAALMSIQAIKGVEIGLGFTAATLPGSQVHDEIFAGKD
                     GFIRSSNHAGGLEAGMTNGQNLVLRAAMKPIATLYNPLRSINLESGQQAEAAVERSDI
                     CAVPAAAVVGEAVTAFEMAVAFMEKFGGDSISEIEQRF"
     gene            12583..13656
                     /gene="aroB"
                     /locus_tag="P5549_03765"
     CDS             12583..13656
                     /gene="aroB"
                     /locus_tag="P5549_03765"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRW12221.1"
                     /translation="MKTIQVDLGEKSYPIHIGEEIMIHFPDMLGRVSSRSVSLLVSDE
                     LVFSLYGPTVLDILRGAGYRVEAAVIKGGESCKNLTTLDWLYEQMIICGLDRSSTVFA
                     LGGGVVGDLAGFAAASYMRGVGYIQLPTSLLAQVDSSVGGKTGVNLPRGKNLVGAFYQ
                     PGLVLADVSLLHTLAEKEYLTGLAEVIKYGVIWDTAFFEYLETNLASIKTRDTECLIH
                     IVERCCAIKAEIVSQDEKETGIRALLNLGHTFGHAFEALTGYEQFTHGEAVAIGMVCA
                     ARLAKNIGMLTAAEAIRITELINNYGLPVDYGNLSADAIVERMRSDKKNTAGQISLVL
                     PAAIGQSQVCTGISDEQIKAVLK"
     gene            14280..15311
                     /gene="aroF"
                     /locus_tag="P5549_03770"
     CDS             14280..15311
                     /gene="aroF"
                     /locus_tag="P5549_03770"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRW12222.1"
                     /translation="MILVMNIDSTREEITAVNERLENMGFQNQVIHGVKKIVIGAVGD
                     PLTSNALKELTQMPGVEKIVPIMKPFKLVSREAQAEGSVIEVGNMKIGGQELAVIAGP
                     CAVENREQMLQAAWAAKDAGAAMIRGGAFKPRTSPYSFQGLEEEGLKLLREAADVTGL
                     PVVTEVIDEHSLHMALDYVDMLQIGARNMQNFRLLKAAGQTDLPVLLKRGMSATIEEW
                     LMAAEYILSEGNQKVILCERGIRTFETYTRNTLDLCAVPVVKRLSHLPIIVDPSHGTG
                     DRQLVGPMSMAAIAAGADGLILEMHPNPAKAVSDGAQSLTPAGLKELMGQLQVLAPAV
                     KRTLGEGGH"
     gene            15313..16161
                     /gene="pheA"
                     /locus_tag="P5549_03775"
     CDS             15313..16161
                     /gene="pheA"
                     /locus_tag="P5549_03775"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRW12223.1"
                     /translation="MAEKIAYLGPGGTFCEQAARQYRGEKELDLLPLPSLRQVFQAVA
                     AGDVCQGIVPIENSYEGAVNQTLDLLTGDDDVKIAGEIILPVRQNLLIHPHSSGQKID
                     CILSHGQALAQCQEYLHRHFPDVPVREVHSTAEAARMVATSGETWAAVATLTAAENYG
                     LKVLAADIQDQSNNATRFVVLSRESTDCQSGGKTSLLVYVVDRPGALYEVLREFYIRG
                     INLCKIESRPARTRIGEYLFFIDIEGSRQEEHVSQTLTALLKVAAEVRVLGSYPDSCY
                     TGTFIV"
     gene            complement(16621..17523)
                     /locus_tag="P5549_03780"
     CDS             complement(16621..17523)
                     /locus_tag="P5549_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRW12224.1"
                     /translation="MDKKITTYLALTLTILFWGLSFIATKIALTSFTPFVYMFFRFTL
                     ASLFFLVLMFWRGFPKLSAGDHKKLFLIGLLEPGLYFIFETFGLTYTTASKASLIIAA
                     VPIAVLLLARIVLKEKITGKRLWGVILSIAGIMILILGDPKFSISSAGSMLGDMLIFG
                     AVISASFYMIIARDLGQRLSSLEITSFQVFYGAILFTPFFLLNMGGMQWSQISAQAVG
                     AVVFLAVFATILGFLFYNYALTQIPASQVAVFINGIPVVTAIGAWFILGESLTFVQMA
                     GGLLVLVAVIVANKPQTSRQASSG"
     gene            18083..19012
                     /gene="fba"
                     /locus_tag="P5549_03785"
     CDS             18083..19012
                     /gene="fba"
                     /locus_tag="P5549_03785"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRW12225.1"
                     /translation="MPFVTTAEMFKKAYEGGYAIGAFNVNNMEIIQGITEAAKEEYAP
                     LILQVSAGARKYANHTYLMKLIEAAVIETGVPICVHLDHGDSFELCKSCIDGGFTSVM
                     IDGSHFSFAENIKLTKQVVDYAHDKGVVVEGELGRLAGVEDAVNVSAKDSSYTDPDEV
                     EEFVAKTGVDSLAIAIGTSHGAFKFKGEPNLRFDILEEVQRRLPGFPIVLHGASSVIP
                     EFVDMINTYGGKMSGAQGVPEDMLRKAASLAVCKINIDSDLRLAMTGTIRKYLADDPA
                     EFDPRKYLGPAREAIKQLVKHKIVNVLGCNGKA"
     gene            19154..19936
                     /locus_tag="P5549_03790"
     CDS             19154..19936
                     /locus_tag="P5549_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12226.1"
                     /translation="MLNSSQYKDIIIGQGIRTLPGWKMPVHLFLVEGLLIDCGPEIMK
                     GPIRKFLQDKHIAQVALTHLHEDHSGMAAWMQRHHHVPVYLHEKVIPEARQKGKYPLY
                     RHITWGKRPAFDPLPMPNRLVTDKYSFDVIDAPGHMRYHHVFHEKNQGWLFSGDLYLG
                     TKPVGACFDDNMLDTIASLKAILTLDFDTIFCAHSGVVENGKAMFQKKLDYLLGIQTK
                     VRALRQQGLDNHAIDKRLYPHKLPVTTFTRGEWSSYHLVNTI"
     gene            20630..21226
                     /locus_tag="P5549_03795"
     CDS             20630..21226
                     /locus_tag="P5549_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12227.1"
                     /translation="MSGQDTGWANPSPAGLVALAVAVITIYATLAGKVDHTAAIYIAM
                     WLLGGFVIQFAVAIIELKEGSLTGGNVFLFFAGMFMLSGAMNDVISFLAGINGITLQG
                     TINGYGWLILTIALTTWMPAYLKTAPAVFSLTVVDLVVMAWCVTFIKLGLAGAILVPI
                     AAYTALIAAVGALWCAAAFILNPAFGREIVSLGGKLLK"
     gene            complement(21394..21470)
                     /locus_tag="P5549_03800"
     tRNA            complement(21394..21470)
                     /locus_tag="P5549_03800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21434..21436),aa:Pro,seq:tgg)
     gene            complement(21541..22146)
                     /locus_tag="P5549_03805"
     CDS             complement(21541..22146)
                     /locus_tag="P5549_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRW12228.1"
                     /translation="MKKAVPPVSQTSGPVINVHVDTRILTLIIQHSLTVYPVAVGKTS
                     TPTPPGHWKIVFKSVNPGGPFGARWMRLSVPWGSYGIHGTNNPKSIGKAVSHGCIRLY
                     NEDVVVVYRQVPVGTPVNIDGVVRKIRTLKQGCKGDDVRQVQSLLQDLGEYTASVDGW
                     FGPLTRQAVIRFKRAQELKATGIVGKDTLKAMQIAHDLSIS"
     gene            22352..23362
                     /locus_tag="P5549_03810"
     CDS             22352..23362
                     /locus_tag="P5549_03810"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRW12229.1"
                     /translation="MKASENGGSRWQPVWLLMIVSLLVMGFMVWNVMVVQNGILKQAE
                     KIYLREQTQNITAVLGEVGQQAEKMAMQIAAQDQLMKAVEQNDNDVLADKLVPLYRQW
                     KQNQGVTELSLVSAAGEAVWSSLNGVEPGNDLSYQRTTSQSLHQQKSVAAMEAAERET
                     MLVVTWPLFMQDEYLGLCRVGISLSYLGEQLQQRQPGQVALFNLNGIDSQLLWENQKG
                     WSALNTNDIKKLQDGETISRPFDWYTRLLVVPLQDIDRMTVAYIQNQLSLQGFVQARL
                     FNYLMLLLALLLICLTNYAYGRSRYYAEDDLSNSLARISKINNVAFQVHPDNSRKNSP
                     DE"
     gene            23556..26465
                     /locus_tag="P5549_03815"
     CDS             23556..26465
                     /locus_tag="P5549_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRW12230.1"
                     /translation="MSFLPLNDPCPCGSGKEYGQCCAPGAGCQVIHFPRGKRNNFRAI
                     IEEALGDLILYARRYFPNWDSVAQAKFLSYSQGGGEIHQKFAHIFWQWYVLNYRFYSD
                     VSPLIDFYLVEMEDILTERMKTVYAALQHSYLSIYTVSWVRNNTVAVTDLFCGDEHII
                     ERDFGSVTQFIEQGSLLYGRIVKLENASIVIGRPVIVTAEQKSYLLDEVNAVYLSENS
                     RTREDMREFLRECAEVTAGLVMDVVQGIKKNRVKSKTLHLESRARKALLYKLNNSQHF
                     DMLERHNHWLKFTWKEGQGIFKRLYVGEKALLVSADESADILYAARLLEEMLAVEQTE
                     FVWQDGIILDDGEQEEDIQTELMVDKNLEDWLKLPHPELADMTPLEAMQDIKGRVLLE
                     NLLTDMEMLELIARSRGEYNYPTAVIRRTLGLDKNALSREMSNPQAIAIKVEKLRNRQ
                     LLSSYVTAYNWLSSEYAQVAATIYDIYSNGGMDQRRLAWLLYLWCEFTTVYRPRVSRI
                     HNWIAALEFTLSNSLGEDISYTKLSRAFGISTGMVSRSAYIVNRHFEKYPPNFKTEMI
                     HYPSWEELDHYEMVQSYEEVYHHLSIYSYTIGNRDPKTKTEVQTAYYQPVNTRARFWD
                     ELNKKIYGDFFEHHYLLDHINANGSTIMNTFWDNQANRFPPYLREAAFRFMMSYVGAY
                     RIKPAGKNSLIFEDVFSGEEFEVYGRFGDNVHENIVPGMIGICRLLPLEKLRWVSDPM
                     FIVLQDMQDIFERNLEIMTENLAGYDASDPLYLKKRGEFLVKAYIRSIDEFEQAALNL
                     VNQPLQPEWQYAYVSCYEKAHELISSCKHFRPLYQDHSRSSYMWDRFSVQGNYQWGYL
                     LVKDNAIIISAPPGKDMHKFTKDIRRAFKCADLVLAFRPAELSRKVLKELEVYMVADL
                     ANYFDQNPAQSLILLRQDTFNNEEKEWQQGIFLLKLGSLLMDYLENKKKNETKQR"
     gene            26650..26724
                     /locus_tag="P5549_03820"
     tRNA            26650..26724
                     /locus_tag="P5549_03820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26682..26684,aa:Gly,seq:gcc)
     gene            26890..28242
                     /gene="tig"
                     /locus_tag="P5549_03825"
     CDS             26890..28242
                     /gene="tig"
                     /locus_tag="P5549_03825"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRW12231.1"
                     /translation="MSARLEKIEKSEAYLSIEVDAQTFEEGLQKAYRKVVKQVTIPGF
                     RKGRVPRELLEAHFGREILFEDAIEMIVPEAFEQALTELDIEPIAQPEFEIEEIKEDN
                     ILRFTAKVPVKPEVVLGQIEGLEVTIPILEMDDADVDRRMEDMANHYAELVEKTEEPA
                     EMGDTATIDFEGFVDGVAFEGGKGEDYPLELGSQSFIPGFEEQVVGLKVGDTADVMVI
                     FPETYQAEELAGKNAVFKVTVHRIETKQPRELNDEFAQEVSNFETIAELREDVQKSMQ
                     ESMDNRRLGFIRDAVIAKALENTQVDVPGAIVRMQLQNMLNQFAEQLKMQGLSIEQYF
                     QLTGRNVDRFNEEMWPEGEKNARVNFMLEKITEEKGIELSDEELDNHIIELSTSMGID
                     PDQARQNLAGVMDDFIYRLKIQKAIDYLVEHAVVTETKDDEEGADTVPASAVEVEPEA
                     "
     gene            28297..28890
                     /gene="clpP"
                     /locus_tag="P5549_03830"
     CDS             28297..28890
                     /gene="clpP"
                     /locus_tag="P5549_03830"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRW12232.1"
                     /translation="MSYLVPMVVEQTNRGERSYDIYSRLLKDRIVFLGSAIDDTVANL
                     IIAQLLFLEAENPDKDINLYINSPGGSITAGMAIFDTMQVIRPNVSTICVGMAASMGA
                     FLLAAGEQGKRFALPNSEVMIHQPSGGMEGQATDIEIRTRRILHMKETLNRILAERTG
                     QDLEKIRRDTERDYFMTAAEAKEYGLIDEILTGPKNS"
     gene            28923..30173
                     /gene="clpX"
                     /locus_tag="P5549_03835"
     CDS             28923..30173
                     /gene="clpX"
                     /locus_tag="P5549_03835"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HRW12233.1"
                     /translation="MPKHGDEKGQLKCSFCGKTQEQVKKLVAGPGVYICDECIELCNE
                     IIEEELSDDIGFELGEIPKPQDIREIMDDYVIGQDTAKKSLAVAVYNHYKRINLGMKL
                     DDVELQKSNIMMLGPTGSGKTLLAQTLARILNVPFAIADATSLTEAGYVGEDVENILL
                     KLIQAADYDIEKAEKGIVYIDEIDKIARKTDNPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFIQIDTSNILFICGGAFEGIDKIIQSRIDEKVMGFGADIKRHNEK
                     KIGDILNQILPQDLLRYGLIPEFVGRVPIIVTLDALDKDALVQILTEPKNALVKQYKK
                     LFELDGVDLEFMEDSLLAIADEALRRNTGARGLRAIIEDVMLEVMYDVPSRLDVTKVL
                     ITKDVIEKKEKPLLVTVDAKRKVN"
     gene            30536..>31044
                     /locus_tag="P5549_03840"
     CDS             30536..>31044
                     /locus_tag="P5549_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW12234.1"
                     /translation="MNSFFMQYGGILYLVQLFFAVVIGLYFWNLLKTQQKGKTVMVHE
                     SEKERNRLRAMRSIKLTEPLSSLSRPGSFQDIVGQEEGISILRSALCGPNPQHVIIFG
                     PPGVGKTAAARLVLEEARQNALSPFNPNASFVEMDATICRFDERNIADPLIGSVHDPI
                     YQGAGAMGTA"
BASE COUNT         7776 a         7597 c         8480 g         7191 t
ORIGIN      
        1 ccaacgaatg acggagtcaa agtccgttgc cttaccgctt ggctacaccc caacagttta
       61 agtgtgtaca tcttgtccct gcctggtgtc ttgccaaacc ccaacgctac tgagtatact
      121 aggatacgcg tacggatgtc aaggggtaat ttggggtgga tgatgggact cgaacccacg
      181 gcccccagag ccacaatctg gtgctctaac caactgagct acacccacca tgacctcaga
      241 cacagaaaaa atggcgtccc cagagggatt cgaacccccg acccacggct tagaaggccg
      301 ttgctctgtc cagctgagct atagggacaa aaaaatggag cgggtagcgg gaatcgaacc
      361 cgcgcaacca gcttggaagg ctggggctct accattgagc tacacccgcc ggcaaaaaac
      421 attatacttc ttttcaaggg gcatttcaat cataaaatca actattttgt aacagtgccc
      481 atgaattgcc agcatcttat attatatgcc ggaatttatg aattatacca gacgattgcc
      541 gggcaataat aattctgtat agcatcttgc cggtggcttc gcttgagcct ggtgcggcag
      601 gcttggtctt gcagcacctt tctatcatcg ggttatgacc gagctgcgcg ggaggagaag
      661 cggagcaaat acggcggccg tgatagaggg ccagcgggag agaaggcctt aatgcaaaat
      721 atttcaatat tgtatcatgc cgggaagttc tttatcattt tgatttagac cagaaatgaa
      781 gaccaaagac aggaataggt ttgactatta ctctctatga ataaagaggt gttttatatc
      841 aagctactgt cattactggt cgatggcgaa gaaatagcca gcaaatacca ataaattttt
      901 ccaggaggtg ttccctgatg cgctgtgatt atcatataca ttcgtcaatt tcggctgaca
      961 gttcaacccg gccggagttg caggtcaagc aagcggaagc gctgggcctg gaggaaatct
     1021 gttttacgga acatttagag atccattttt accgcgggga tgcctggcat gtgaatgtgg
     1081 cggagtaccg cgagcaattc cgccgtctgg cgtccgaccg ggtcaagcta aaattcggag
     1141 tggaggcggg catcgcgctg gcgtcggaac attttcccga attggagggc gagctgcgcg
     1201 cggcggactt tgattttgtc ttggctagtg cccattcgat tgacaatatc gatccttttg
     1261 atccgcgttt tttcgaaggc aagaccctgg agcaggtttt tcgaaactat attgcttcca
     1321 ttctggccgg cctcaagcag ctcaatacgg agctgtttag cagtgtaggg catatcgatt
     1381 ttccctccaa gggagctcac tcagaggagg acgcccggct tttttatcgt tatgccgcgg
     1441 atgagatcga taccttgttt cgctatctga ttgcgcaggg gaaatgtatt gaaattaata
     1501 cggccagtta ccgccagctg aacggtctgg cgattccagg ggaggattgg ctgcgtcgct
     1561 acgcccagct gggcggggaa tatgtcaccc tgggttccga tgcccattcg ccggagttta
     1621 tcgcctatcg gctggaggag gcaacggaga tggccaaacg ggcgggaatc aaatactacg
     1681 ccacttatga acaaatggtg ccggtattcc acaaactgta aagaggcggg tcagactgta
     1741 ttcaggactg gattaaacac tccagattgt tttacaatgg tagacaagga gtgatttcat
     1801 ccatgaaggg agaagtttac aatcaatcca cctggattgc tttgaaggcg gccagctact
     1861 attacgaaca caacctgtcc ctaaaagaga tcgcccgtct gttgaatgtt tctgaagcaa
     1921 cggtgtccag actgattcgc cgggcccgga aggaacagat cgtggagttt gtgatccgcg
     1981 atccctacaa acagtgcctg gacctggaac atcgcttgca ggatggattc gggctgaagg
     2041 cagtcattgt taccgcacct gccgcgcggc aggacccgga agaggataaa aagcaggtgg
     2101 cgctggaagg cgcccgatat ctgcagcgta tcattaccgc ccatgatgtg ctggggattg
     2161 cctggggcgg taccatgtat tatctggtac attacttgaa tccctgccag cggaccgata
     2221 ccatctttgt caccctgcac ggcagcctga gctgctgcga ttacgaactg gatgtgcaca
     2281 atctggtttc ccgcatgagc atggcgctgg gaggcaacta ttattctctc tccgccgccg
     2341 gacggttcga tgaggtggag agcctgcagg ccatccgcca tgaacccaat gtcaaacagg
     2401 tcttttcctt gtttgatcaa ctgaccattt ccgtcagcgg gctggggtcc ttctacccgg
     2461 aactggattc gccgctttcc cgtctgcaat atcttcagcc cgctgaactg gctgatctgc
     2521 aggccaaagg ggtttacggt gatatgctgc tgcggttttt taatcgggat ggtcaggaat
     2581 gcgacagtga tgtcaggcaa cgtaccctag ccatcgatta tgcaacctat aagcgcattc
     2641 cctgcaagat agccgttgtt tccggggcgc aaaaggcgca aagtctggcc gcggctttgc
     2701 gcggcaactt gattgatgtc ctggtggtgg atcatcattt ggcccaggca gttctgaccc
     2761 tgccctgaga ccccaaatct aaaccaataa tgaccgtgag ctttgagctg gcagcgttag
     2821 ctgccagttt tttgttgtct tttgccggtt gagcgagcca agcgggtgaa aaaactgctc
     2881 tgcaggcgga ggaaaaatcc tgagagcgtg cttatccaag gacatttatc caatccaaaa
     2941 tctggacggg gtagatggat atctcgtcgc caggatagct gcaaacaaag atctttgtga
     3001 aaagccgcat cggcagaagc accgcagctt ttaccgcagc caatataatg tgaataaatt
     3061 catgtaatat tgaaaatatt ttcaatatcg ccttctattt ttcccctgtt taccatgtaa
     3121 gtataggtac cttaaattaa aacaaaatgg aggaaagaaa atgtatattg accccatcat
     3181 ctgccaattc cccgaagcgt tggatgaaga acgttttatt atcgcaacct attattgtgc
     3241 cgccaaaccc agtaccaaca tgctgaaatt tgctgccgcc ctggcggtgg aacaaaccac
     3301 cggaacctgg ctgaaggtcc ccgcggagac cccggaagtg cgcgaacgct gtatcggccg
     3361 cgtggttggc gtctatgaga cccctgccta tcagatcgaa atccccgccg acgtgcagga
     3421 aagacatttc atcatcagaa ttgcctttcc ctgggccaat ttcggtgccc aatttgcaat
     3481 gatgttaagc tcggtcattg gcaatatctc cagttcgggc aaggtcaagc tgattgattt
     3541 ggagtttccg aaagcgtttc tggcagaatt ccaggggccc aagtttggag ttcagggcat
     3601 ccgcgagctg ctgggcgtgt atgaccggcc gctgctcaac aatatgatca aaccctgtac
     3661 cgggctggac gcagcgacaa ctgccgaact ggcttatgaa tcagccatcg gcggtgtgga
     3721 tatcatcaaa gacgacgaac tggtagcgga tgccccccac tgccggttgc tggaccgggt
     3781 caaagcggtc acggcagcgg tcaaaagggc ggatgaggaa aaaggagaaa agacgctctt
     3841 tgccttcaat atcaccgacc gcaccgacaa attgaaagat aatgcctacc gtgcgatgga
     3901 ggccggtgcc aactgtctga tggtcaatta ttttaccatc ggtctggatg ccacccgcat
     3961 gctgactgag gatcccaaca tcaatgtgcc catcctggcc cactcggact ttaccggcgc
     4021 tgtttacgaa tctccctggt cgggtatgag cgcttccctg attggagcga aactgccgcg
     4081 tctggcgggg ctggacatgg ttatcggctt gtctccctac ggcaaattcc cgattatgat
     4141 ggataccttc attaatctgg gctacaacat gttggcgccg ctgcataata ttaaaccggt
     4201 cttccccatg ccgggcgggg gcaccaccca gggtcatgtg gaagatataa tgaaaaaatt
     4261 tggcaatgat gtgattatcg cttccggcgg cgccatccac ggtcatccca tgggaccggc
     4321 agcgggtgcg cgggcgttcc gacaggccat cgacagtgtc atggcgggca tgagcctgga
     4381 agaagccgga cagaaataca cggaactggg tgtggctctg aatgcttggg gaatttattc
     4441 cgaagcccaa ggcggaattt ttgatttgaa gggctagtcc tgtgtaaaat acaaaaatat
     4501 aggaggtatt acaacgatgg caaaacagta tactcgcgaa caggtgctgg aaagagtcag
     4561 gaaaagtgtt gaggcaggca aaccggtcgt cattgccggg gctggaaccg ggatttccgg
     4621 caagtttgcc gaacgcggcg gtgcagattt gattggtgta tacaattccg gtctgtaccg
     4681 catggacggc aacggctcgc tggccgggct gatgccttac ggcaacgcta accagatcgt
     4741 gattgatctg gcccaccggg tcttcccgca aatccaggaa gctccgatga ttgccggcat
     4801 ctgcggctcc gatccgaccc gggaaatgaa accgtatctc aagtatttgc tcgatctggg
     4861 attttcagca gtgatgaact tcccgaccgt cggcctgatt gacggacgtt tccgccagga
     4921 actggaagat accggcatgg gttacggcaa ggaacttgcc accctgaagc tggccgccga
     4981 gctgggcatg ttcaccattg cctatgcctt taacgaagcc gaagcagaaa tggtcggcca
     5041 ggcgggcatg gatgtaatga tctgccacat gggtcttact accggcggcg ccatcggctc
     5101 caaattctca gaaaaagtga ccctggagga tgccgccgcg ctggtcaacc gcatgtcggc
     5161 agcggcacgc aaaggcaatc ccaacgtgat catttttgcc catggcggtc cgatctcttc
     5221 tccccaggac accgcctata tttatcagca tactgattcc gtcggattcc tgggtgcatc
     5281 cagcattgag cggattccga ttgaagtacc cctgaccgag gcggtcaaag actttaagaa
     5341 ccagtcactg aaaaaataat cggcagatta aggaggcggt tcgattgacg gcgaaaattc
     5401 tggttgcggg aattctggat accaaaggcg aagaaatcaa atatatcgcc cgacaggtcg
     5461 cagcggccgg tgctaagccc accattttgg agctgagcgt gggcaaggaa gtcggctggg
     5521 cggatattgg actcagtgaa atactaaaag aaattggcaa agtacccgaa gagctctaca
     5581 aggtagatcg ccatcaggct tccgcctggg ttaccgaggc ggccattcaa tatgtaggcc
     5641 gcatggtaag tcagggagag gtcgatggga ttattgcttg cggaggttcc atgggcgcca
     5701 gcatagcgac ggccatcatg cagagcatgc ccatcggcat ccccaaattg atggcgacca
     5761 caatgacctc cggggatgtt cggccctatg tcggcaccaa ggacatctgc atgatgtacc
     5821 cgattgccga agccggcatc aataccctca ccaaacggat tctgaccaac gccgccggcg
     5881 ccattgtggg catggccaat tctcctgcga ttgatacttc cgatgacaaa cccatgatcg
     5941 gctgcatgat gttcggcgtg accaccccct gcgtattgcg ggcgtcaaaa acaatggaag
     6001 atcacggcta cgaggtaatg atcaaccacg ccatcggttc cggcggactt tccatggaac
     6061 agctgattga agacggcttt attgaaggca ttatggatat caccacccat gaaatcgctg
     6121 atgaaatgct gggcggggta ctgagtgccg gaccggaccg gctgacagcg gcaagccgca
     6181 aaggcattcc ccaagtggtt gcgcccggcg gattggatct gattaacttc ggtcccctgg
     6241 atacgatacc gaagcactac atggagcaaa cgaacttgcc gggacgctct ctatatgagc
     6301 ataatccggc cgtaaactgt attggtgttc ttcctgatga agcctataaa atcgctgaga
     6361 atatggccat taaattgaat aaatcgcagg gccctacggt tatctgtgta ccgatgcgag
     6421 gctggggagc ctgtgatctg ggggctccga acaaggagct cggatgggca ggtcctgcag
     6481 ctggaccgag ctgggtggct gacccggaca aagcggaatg gtcgctcaga agccggcggt
     6541 ttatggacgg gatgaaagag ttccttgatc tcagcaaacc caatattgaa ttgctggaag
     6601 tcgacaaaca tatgaatgaa cctgaatttg cagatctgat gtcggggctt ctcaaggaca
     6661 tgctggacgg caactggaag aaaaattccc gcagtcatct atcctatgtc cactcgattt
     6721 gagcgggggt gcggtaatgg cacagaaatt ttctcggcaa cagattttgg aacggctgca
     6781 cgggcagatc aaagcggggc gtcccctgct gatgttcggg gctggcaccg gtctgacagc
     6841 caagtgcgcg gagaaggggg gcgccgacct gattggggtg tactccaccg ccatctaccg
     6901 gatgcggggt ttgccgtcgc ttttggcctg gatgccctac agcaacgcca acgaacattt
     6961 gctcaacatg gccggagaga ttcttcccat cgtgcagaca acgccttgca tcgccggcat
     7021 cggtgcccat gatccggcgc tggatttgga tgcattcttg gatacggtgg tgcaggcagg
     7081 tttttcgggg gtgaccaatg aaccttttgt aggcttgtat ggcaagtatt ttgctgacca
     7141 gttggagcgg gccgggctgg gattttcgag ggaagtcgag ctaatccgcc gcgcccgtgc
     7201 caaaaatatt ttcaccgtgg cctgggccat gtcgccggaa gaggcttatc aaatggcaca
     7261 ggccggcgcg gatgtcatcg gagccatgat cggcgtgacc agcggcggga tgtcaggggc
     7321 ttcagacgtg atttccctgg aggaagcggt acaggatgtg cgggatatgg tcgccgctgc
     7381 ccggcaggct gatccccagg cgctgttatt gacgcatggc ggcccgttcg ccgatgttgc
     7441 caccgcccag tattcgattc agcatactga tgcggtgggt tatgcttccg gttccagcgg
     7501 tgagcggatt cccaccgaag aagcggtgat tggcattacc ctgcagtaca aagcgatgca
     7561 gctggaataa aagatgcaaa cggcaatctg tcagaggtgc tgttgttgag ccgcagattc
     7621 aaacaggaga aatcctgaga atatatatcc aagaccgccg caggagcccc tctcctgcgg
     7681 cggtcttggt ctgtttcctt attatttttc cagcagccag gactgaaacc cgtgctgctt
     7741 taaaatctga caggcgcccg gagcgctttc ccgtggtacg gtgattttga tactgccttc
     7801 gtcctcgtcc cggacgttca acagctgcag gttctggatg ttgatgttgg cctggcccag
     7861 cagtgttcct accgtgccga tgaccccggg ctgatcgggg atgatggcga ttacatcgca
     7921 ggcctctgcc agacaggggc gcaggccggg caggtttttc cgggtggtgc tggctgattg
     7981 cagatacgtg cgcagcaccc catcgccttt gcgctgcagg ctgtgccggt aaatgctgag
     8041 caattgctgt atcgccgcca attcctggcc gatggcatct gcattcagga acaggatatc
     8101 ctcccacaga tccggatcag aggccgccgt gcgggtactg tcgcgcagac ctccgccggc
     8161 cagattcata aattccggtt gggtcagtaa attgttgatc agtgccgtcg acatcagatg
     8221 gggcagatga ctgactcggg ccacggcctg gtcatgttcc gccgcattca tgattaccac
     8281 ccgggcgccg atggcagtga gcaactcttg cagttgtgcg atgaccgctt ccggcgtacc
     8341 ggtccggggg gtcaaaatat aggcggcgtt ttccagcagg aacttgtcag cggcctgcaa
     8401 accacttttt tccgaaccgg ccatgggatg accgccaaca atgaacacgt cggcggggaa
     8461 atgcgcctcc aattgctgca tgacccagcc tttgacgctg ccggcatcgc tgatgatggc
     8521 gcccggtttg acatgcgctt tgatttgttc catgacagcc ggcatggcag tcagcggagc
     8581 agccaggatg accagatcgc attgcgccag gaaacggtcg tccagcatct gatcgatggc
     8641 acccagttcg atggcctggg ccacggttga ttcgtccaga tcgcgtcccg ctacgatgac
     8701 gcccagaccg ttgctgcgca gagccatgcc cagcgaaccg ccaatgacac ccagaccgat
     8761 tatgccaatt ttttgaatca ttgtccgaac cccgctgcag gatctgttgc cggtgcagta
     8821 aggctctcca gccgccggac tttgctgctc agccgggcga agtcctctgg aaacagggac
     8881 tgtggtccgt cactccaggc ctgggccgga tcgggatgga cttcgataat taatccgtcg
     8941 gcgcccgccg ctaccgaagc cagtgacatg ggttccacca gttcgcgctt gccggtgccg
     9001 tgactgggat cgacgatgac cggcagatgg gtcagctgct tgagcagcgg gaccgcgctc
     9061 aaatccaaag tattgcgggt gtaagtctca aaggtgcgga tccctctttc acacaggatt
     9121 acgttgggat tgccgctgcc cagtatatat tcagccgcca tcagccattc ttcaatcgtg
     9181 gcggacatgc cgcgcttcaa caaaattggt ttactgacct tgccgagttc ccgcagcagg
     9241 aagaagttct gcatattgcg ggcgccgacc tgaatcatat ccacatattc ggccagcatg
     9301 gggatggact tctgatccat tacttcagaa accagcggca gtcccgtcag ttcgcgggct
     9361 tccgccatga tttgcagtcc tttttcaccc agaccctgaa aagagtacgg ggaagtgcgg
     9421 ggcttgaatg cgcctccgcg cagcatttgt gcaccggcat ccttgacggc cgctgccgcc
     9481 tccagaaagt tctcccggcc ttcaatggca cagggcccgg cgatgagcgt tacatgactg
     9541 tcgccgatga gttgcggccc cacctggacg acagtatttt gatgtttgaa atcacgggac
     9601 gccaatttaa acggctgcat gatgggcacg actttatcca ccccgggcag ggatgccagg
     9661 gtctggggat cgatctggcc tggttcaccg atgacgccca tgatggtgcg tttggctccc
     9721 tgggacaggt ggacgctgta accctctgcc tccaggtgtt tttgcacctt tgcgatctgt
     9781 tcctgactga cacctttttg catgaccact accatactac ttacctcctc aaatttgcgg
     9841 gcaaaaagca gaaaaaccat ggcaaagcgc atggttacac agtgatccgt agatcgatat
     9901 gtgcctcccg tcctgccttg ctgccctttt tataaacaat gactccagta aaaccatcct
     9961 acaaaatgaa tcataccaca ggccgggcag aaatcacaag caaataaatt ttcatctggc
    10021 tttatctttt gtcgttttcg tatataataa gatattaatt attgccatta taatacccga
    10081 caaattatga gggattatcc tgatctggag gtgtcgcaaa tggacctgac agtcaaaccg
    10141 agtcagcctt ttcgcgccgt tttgcgcgtt cccggagata agtccgtttc ccaccgcagt
    10201 gtgatgattg ccgccatcgc taatggcgtc agtaccattg acaatttcct gcccggcgat
    10261 gactgtctca gcacggtccg ctgcatgcgg cagttaggag taaaaattga agaactcacg
    10321 cctacccgtt tgcaggtgca tggctgcggt ctgcacggcc tgcaggaggc aggggactat
    10381 ctggatgtcg gcaactccgg cacgaccatc cgctttttat ccgggctgct ggccggacag
    10441 ccgtttttct ccgtgctgac cggggatgct tccatccgac gccgccccat ggggcgggtg
    10501 gtcaagccgc tccggcagat gggagcgcag atctgggggc gggacggaga ccgtctggcg
    10561 ccgctggcga ttcgcggcag ccatttgcag ggcatccatt tcgacagtcc ggtggccagc
    10621 gcccaagtga aatcagccgt cttgctggca ggattatatg cccgggggga aacctcggtg
    10681 actgagccag cccaatcgcg cgatcatacc gaaaggatgc tggccgggtt tggcgcggat
    10741 attaaaacgg aaggactgac gacggtcatc cgctccgggg agttggccgg acaaccggta
    10801 ctggtgccgg gggacatttc ctcggcagcc tttttcctgg ccgcggcagc cattataccg
    10861 ggcgcccgga tcacagtaca ggacctggga ctaaacccca cccgtaccgg tatcattgaa
    10921 gtactgcagg ccatgggggc gactgtcgtc atcgacaacc ggcgcaatgt ttccggtgag
    10981 gaactgggcg acgtgtcggt gaccggcggc gcgctgaaag gcttggagat tggtggagag
    11041 atcattcccc gtttaattga tgaaatcccg gtgctggcag tggttgccgc ggtggcggag
    11101 ggacaaaccg tgatccgcga tgcccaggaa ctgaaagtca aggaaagcaa tcgcctgcag
    11161 gcagtagcta ccgagctacg gcgctttggg gcggatatac aggaaaccgc agacggtctg
    11221 gtcattcagg gggggcgtca gtcctatcac ggcgcagtct gcgagtccta tcatgatcat
    11281 cgtattgcca tggccagtgc ccttatgggt ctggtcagta aaggtgaaac caccgtgcac
    11341 gacgcggaat gcatcaacat ctcttttccg ggttttgcac acctgctggc tgaattggga
    11401 ggaggagaag gatgctgaga tacttgaccg ccggggaatc acacggaccg gccttgaccg
    11461 ctgtcgttga aggcataccg gcgggattgg aactgactgc tgattttatc aaccgccagc
    11521 tggcccgccg ccagaagggt tacggacgag gcggacggat gaagattgaa accgatcagg
    11581 ctgacatcct ctccggcgtg cggggcggaa aaaccctggg cagtcccgtc acgttgcaga
    11641 tcaagaacca cgactggcag aactggtcgc agatcatggc cagcggcgag gcggacttgc
    11701 agtcgcgccg ggtgagcgta ccgcggcccg gacacggaga tctggccggc catatcaaat
    11761 acggccatga cgatttaagg aatatcctgg aacgcgccag tgcccgcgaa accgctgccc
    11821 gggtggccgc ctgcaccgta ggccgcagat tgattgaaca cttcggcatg gaaatccaca
    11881 gccatgtcat ccaaatcggc agtatcaagg cccaaccggc tagtctgcag gagatcatcg
    11941 atcgcgcccc acagtcggag ctgttctgcg ccgatccgga agctgaagcg ctgatgaaac
    12001 aagaaatcga tcaggcccgc gaacagggtg actccctggg aggcattttt gagattatcg
    12061 tctgtcatgt accggtgggc ttgggcagcc acgttcactg ggaccgcaag ttggacggcc
    12121 ggctggccgc agctttgatg agcatacaag cgattaaagg cgtagaaatc ggcttgggat
    12181 ttaccgctgc cactttgcca ggttcgcagg ttcatgatga aatttttgcc ggcaaagacg
    12241 gatttatccg cagttccaat catgctggcg gtttggaagc cggcatgacc aatggacaaa
    12301 atttggtgct gcgggctgcc atgaaaccca tcgctacgct ttacaatccg ctgcgcagca
    12361 tcaatttgga aagcgggcag caggctgaag ccgcagtgga acgctccgac atctgtgccg
    12421 tcccggccgc cgccgtggtc ggcgaagcag tcaccgcctt tgagatggca gtggctttca
    12481 tggaaaaatt cggtggtgac agcatctctg aaatcgaaca acgtttttga taaattgtga
    12541 cagggggaag taccacatat tttgctataa agcaggtgac atatgaaaac catacaggtt
    12601 gatctggggg aaaagtctta ccccatccat attggcgaag aaattatgat ccatttcccg
    12661 gacatgctgg gcagggtgtc gtcgcgcagc gtctccctgc tggtgtccga cgagctggtc
    12721 ttcagcctgt acgggccaac ggtgctggat atcttgcgcg gggcgggcta tcgtgtggaa
    12781 gccgcggtga tcaaaggagg cgaaagctgt aaaaatctga ccaccctgga ctggctttat
    12841 gaacaaatga ttatctgcgg actggaccgc agttctacag tgtttgcctt gggcggcgga
    12901 gtggtgggag atctggccgg ctttgccgca gccagttata tgcgcggggt cggctatatt
    12961 cagctgccca cctccctgct ggcgcaggtg gacagcagtg tgggcggcaa gaccggcgtc
    13021 aacctgcccc ggggcaaaaa cctggtgggc gccttttacc agccgggatt ggtgctggcc
    13081 gatgtcagcc ttttacatac cctggcggaa aaagagtatc tgaccggact ggcggaagta
    13141 atcaagtacg gcgtcatctg ggacactgcc ttttttgaat atctggagac caacctggca
    13201 tccatcaaaa cccgcgacac ggagtgtctg atccatatcg tggagcgttg ctgcgccatc
    13261 aaggcagaaa tcgtcagcca ggatgaaaaa gagaccggta ttagagcatt gctgaatctc
    13321 ggccatactt tcggccatgc ctttgaagcc ctgaccggat acgagcagtt tacccacggt
    13381 gaagcggtgg ccatcggcat ggtctgcgcc gcccgtctgg cgaaaaatat cggcatgctg
    13441 accgcagctg aagccatccg gatcacggaa ctgatcaaca attacggcct gccggttgat
    13501 tacggcaatc tgtccgcgga tgccattgtg gagcgcatgc gcagcgacaa aaaaaacacc
    13561 gccgggcaga tcagcctggt actgcccgcc gccatcggcc aaagccaagt atgcacaggc
    13621 atcagcgacg agcagatcaa ggcggtttta aagtaagggt gccagacgcc aggatcagca
    13681 aattgatatg ccacctgctc ggcgcgatgg atgcattaat tcaaaaaaat acttgcatat
    13741 gacaggtcac atggtataat cattactaat tcatattcaa caacgatgaa cgagcaaagt
    13801 acgttgaccc cagctttcca gggagaaggt gccatcggct ggaagcacct ttaaagcatg
    13861 attgaccgaa gttctctcgg gagttgccag ctgaagtctc gcaagagatg gtaggcccgg
    13921 ccgtaatctt cccacgttaa agggaaggag gtatcggaac agatcgttcc cgcacctttc
    13981 aatgagcgga tctgcattaa tgcgggtcaa ccagggtggt aacgcggcaa gcttgagctt
    14041 ttcgtcccta gcggatgaaa ggcttttttg tttcccgttt ttaatgattt gattcccgta
    14101 cctcggggtt ggtaacggaa tgccggtggc gtgcctgccg gcagattttc ccgcaaccac
    14161 ccctacaatg agtgggtctg cagctgcaga tcaatcaggg tggtaccacg gatgcccata
    14221 gctttcgtcc ctgcagggag aagagctatg ggcaattttt atattgggag gaagaaaaaa
    14281 tgatactggt tatgaacatt gattccacga gagaagaaat cacggctgtc aacgagcgac
    14341 tggagaatat gggtttccag aatcaggtga ttcacggagt aaagaaaatc gttatcggtg
    14401 cagtaggcga cccgctgacc agcaatgcct tgaaggaatt gacccagatg cccggggtgg
    14461 aaaaaatcgt gcccattatg aaaccattca aactggtcag ccgcgaggca caagccgaag
    14521 gctcggtgat cgaggtcggc aacatgaaaa tcggcgggca ggagctggcg gtaattgccg
    14581 gtccctgtgc ggtggagaac cgcgagcaga tgctgcaggc ggcctgggcg gcgaaagatg
    14641 ccggcgcggc catgatccgc ggaggcgctt tcaaaccgcg cacctcacct tacagcttcc
    14701 agggcctgga ggaagaaggg ctgaaactgc tgcgcgaagc ggctgatgtg accggactgc
    14761 cggtggtgac cgaggtcatc gacgaacaca gcttgcacat ggcgctggac tatgtggata
    14821 tgctgcaaat cggcgcccgc aacatgcaaa atttccgctt gcttaaagcc gccgggcaga
    14881 ctgatctgcc ggtgctttta aaacgtggca tgtcggccac cattgaagag tggctgatgg
    14941 cggccgaata tatcctgtcc gaaggcaacc agaaggtcat cctctgcgaa cgcgggatcc
    15001 gcaccttcga gacctatacc cgcaacaccc tggatttgtg cgcggtaccg gtggtaaaaa
    15061 gactgagcca tctgccgatt atcgtcgacc ccagccacgg caccggcgat cgccagttgg
    15121 ttggacccat gtccatggct gccattgctg ccggcgctga cggactgatt ctggagatgc
    15181 accccaaccc ggccaaagcc gtatctgatg gcgcccagtc tctgactcct gctggattaa
    15241 aggaactgat gggacagctg caggtgctgg ccccggccgt aaagcgcacc cttggggaag
    15301 gaggacattg atatggcaga gaaaatcgcg tatctgggac cgggcggaac cttctgtgag
    15361 caggccgccc ggcaatatcg gggcgaaaag gaactggatc tgctgccgct gccttccctg
    15421 cggcaggtgt ttcaggccgt agccgccggc gatgtctgcc aagggattgt gcctattgag
    15481 aattcctatg aaggcgccgt taaccagact cttgatttgc tgactggtga tgatgacgtc
    15541 aaaattgccg gcgaaatcat tcttccggta cggcagaatt tactgattca cccccactcg
    15601 tcaggacaaa agattgattg catcctctcc cacgggcagg ccctggctca gtgtcaggaa
    15661 tatctgcacc gccatttccc ggacgtgccg gtgcgggagg tgcatagcac cgcggaagcc
    15721 gctcgcatgg tggcgacttc gggagagacc tgggcggcag tggcaaccct gacagcggcg
    15781 gaaaactacg gcttgaaagt gctggccgca gatatccagg accagtccaa caatgccacc
    15841 cgctttgtgg tgctgagccg ggagagtact gattgccaat caggcggcaa aacctccctg
    15901 ctggtttacg tggtggaccg gcccggcgcc ctttatgaag tgctgcggga attctatatt
    15961 cgcgggatca atctgtgcaa gatcgaatca cgccccgccc gcacgcgtat cggggagtat
    16021 ctgttcttca tagatattga aggaagtcgt caggaagagc acgtcagcca aacgctgacc
    16081 gctctgctga aggtagcggc ggaggtcaga gtgctggggt catacccgga tagctgttac
    16141 accgggacgt tcatagtgta acaaaaatgg atgatctttt gcttccagtt cttttacaat
    16201 ccaatcaata atatgcaata atgagaataa tcagataata aattagcttg ccaatctgat
    16261 tacaaaaagc gggggagggc actgctatca gtctttggca gtggcagagg tagaaaataa
    16321 aatttagaag aattaagcaa tgaacgaggc tggctccaat tcatttaagg attggagcca
    16381 gccttttttc atgcataatg aatcaggggg acggttctcc tgttaccctg aatttttcgt
    16441 gctgccgcac ctcctggagg atctccacaa gattcctcct gggaacaatt gctgcggaaa
    16501 atgcttaagt aagaaaaaca ggacaggagt tttaacacat gaaatctgaa aaaaagtcat
    16561 cgtatttttc atatcattac tgggaatttt tatgggggcg actgaaagcg ggattgtctc
    16621 ttagccggaa gaagcctgtc gggaggtttg cggtttgttc gccacgataa ccgcgaccag
    16681 aaccagcaat cctcccgcca tctgcacgaa ggtcaaactc tcgcctaaaa taaaccatgc
    16741 cccaatggca gtaacaacgg gtatcccgtt aataaacact gccacctgcg atgcaggtat
    16801 ctgggtcagg gcgtaattat agaagaggaa acccagaatg gtagcgaaga cggccagaaa
    16861 aacaaccgca ccgactgcct gggcactgat ttgagaccat tgcatgccgc ccatattcaa
    16921 caggaaaaaa ggagtaaaca gaatggcgcc gtaaaacacc tgaaagctgg ttatttccaa
    16981 ggatgacagc ctttggccga ggtccctggc aataatcatg taaaaggacg cggatatcac
    17041 tgcgccaaaa atcaacatat caccaagcat ggatcctgca gatgaaatgg agaatttggg
    17101 atctcccaga atcaagatca taatgccagc aatggataat ataacgcccc ataatctttt
    17161 tccggttatc ttttccttta aaacgatccg ggccagcagc aatacagcta tgggcaccgc
    17221 ggcaataatt aatgatgcct tggaggctgt ggtataggtc aaaccgaatg tttcaaagat
    17281 aaaatacaaa cccggttcaa gcaggccgat taaaaataac tttttatgat cgccggcact
    17341 cagttttgga aaaccgcgcc aaaacatcag gaccagaaaa aatagcgaag ccagcgtaaa
    17401 cctgaaaaac atgtaaacga agggagtaaa actggttaag gcgatttttg ttgcgatgaa
    17461 agacaagccc caaaataata tcgtcaatgt caaagcaaga taggtcgtaa tcttcttgtc
    17521 cataaacggt ttcctgtctt tacgggagat tcatgactgc agatgagtca ctgagattca
    17581 ttttaacata tctaactccc aacggaaaaa cctgaccgag aaccagatgc aaataacagc
    17641 cagcacgatc atcaccataa tagattgtaa ttataaagcg ggcttcttac catctatttt
    17701 gaatccccca gcttagccgg gttttgtata taccagtcat catattgagc aactaattca
    17761 tcatcagttc cgttaacgcg aggtgtaaac tgacaaagca cggagacctg acggtatatg
    17821 agatcaaact gccgatgcgc ctgtgcctct gccggacgat catggccaag gatggagccc
    17881 atgcgcttgt atctatcggc gcgttttacg acgagctgat tcgcgacgga gtcccgttca
    17941 gccgcgcctg gccggaattc tgtgaatacc ctggcaacaa gcttttgaag ggcgagttta
    18001 aggtggtgat taaagaaatt ttttgtgctg tcaagtggta taataataca tatttgaact
    18061 attgcaaaag gagctggaaa ccatgccatt tgtcacaaca gcagaaatgt ttaaaaaagc
    18121 ttatgaaggt ggttatgcaa tcggagcctt caatgtcaac aacatggaga tcattcaggg
    18181 aatcaccgaa gctgccaagg aagaatatgc gccgctgatc ctgcaggttt ccgccggcgc
    18241 tagaaaatat gccaatcata cctatctcat gaaactgatc gaagcggctg taatagaaac
    18301 tggagtgccg atttgcgtac acctggatca cggcgatagc tttgaactgt gcaaatcatg
    18361 catcgatggg ggttttactt ccgtcatgat agatggatcc catttttcct ttgcggaaaa
    18421 tatcaaactc accaagcagg tggttgatta tgcccacgac aagggtgtag tggtggaagg
    18481 agagctgggc agactggctg gggtggaaga tgcggtaaac gtatccgcca aggactcctc
    18541 ctatactgac ccggacgagg tcgaggaatt tgttgcgaaa acaggcgtgg attccctggc
    18601 cattgccatt ggaactagcc acggagcgtt taaattcaag ggtgagccga acttgcgctt
    18661 tgacatcctg gaagaagtac agagaagact gccgggcttt cccatcgtgc tgcacggtgc
    18721 gtcctcagtc attcctgaat tcgtcgacat gatcaacacc tatggcggca aaatgtccgg
    18781 cgcccagggc gtacccgaag acatgctcag gaaggccgcc tccctggccg tatgcaagat
    18841 caacatcgat tccgatttac gcttggccat gaccggaacc atccgcaaat atttggcaga
    18901 cgatccggcc gaattcgatc cccgcaaata cctggggccg gcccgggagg ccatcaagca
    18961 gctggttaaa cacaaaatcg tcaacgtcct gggctgtaac ggcaaggcat agaatgttta
    19021 acgtgaaaca ttgttgcaac agaaatgcct gaataaacgg atttgtgatt ttaaaaatgc
    19081 aaaagtccat gcaagcatct gtgaagatcg gccaacaaaa ttaacaaacg ctggattaaa
    19141 aaggagtttg caaatgctga acagcagtca atacaaggat atcatcattg gacagggcat
    19201 ccgcaccttg ccgggatgga agatgcccgt tcatttattc ctggtggaag ggcttttgat
    19261 tgattgcggt cccgaaatta tgaaaggacc gatccgcaaa ttcctgcagg ataaacatat
    19321 cgcccaagtg gccttgacac atctccacga agaccactcg gggatggccg cgtggatgca
    19381 gcgccaccat catgttccgg tctacttgca cgaaaaggta atcccggagg ccaggcagaa
    19441 ggggaaatac cctctgtacc ggcacattac ctgggggaaa aggccggcct tcgacccgct
    19501 gccgatgccg aaccggctgg tgaccgacaa atacagtttt gacgttatcg atgcccccgg
    19561 acacatgcgt tatcatcatg tattccatga aaaaaaccag ggctggctgt tcagcggaga
    19621 tctttatttg ggaaccaaac cggtgggagc ctgttttgat gacaatatgc tggataccat
    19681 cgcctcgctc aaagctatcc tgacgcttga ttttgacacc atattctgcg ctcacagtgg
    19741 tgtcgtggaa aacggcaaag ccatgtttca gaaaaagctg gactatctgc tggggattca
    19801 gaccaaggtt cgtgctttac gccagcaggg actggacaac catgcgatcg acaaacgcct
    19861 ctacccgcac aaacttcctg tcacgacctt cacgcgcggc gaatggtcgt cctatcatct
    19921 ggtgaatacg atttaatcat tcatttccaa ccttgcgttc gcctcttttg cttctttgtc
    19981 agggcttgta ctgatccgca atcgacaact atagacaatg ggaaagattt gatgatggga
    20041 gtatccgttg cggtttattc tccggattta taatccttct tcgttgattt caagcgtctt
    20101 gtttcggctt tttttctctg cttcatatgg tagtataaaa aaacagctgg aggaattgtt
    20161 tggaatttaa tttaagatgg tctctattct tgttatctcc ttcgttctaa tgaagcattt
    20221 ctgattcata ccaccaaacc agctgggatt ggcgttatgc cagtcccaga cgcatttaag
    20281 caccacgtat gcctgcttga tacgccaccc caaagattcc tttttcataa aaaaaccgcg
    20341 ccgggatctt aaaaatctgg tgcctgtcgc agcgacaccg ctgaacagca gctttttatg
    20401 cccttgaacg gtaaaagcat gaaaattgag ttctttttca tgacagtcga tcaatatatt
    20461 ttaatgattc tgtttttacc ttttttataa aggtaaatga ggctgcatat aaggggggag
    20521 taatcttggg gaatttaagg aggggcggtg atgtcgtcag attccaggac agacgttggg
    20581 ctttcctggg gaaatcaagt ttcattaaaa attaagggag gaatcattta tgtctggaca
    20641 agatacaggt tgggccaatc cttcaccggc ggggctggtt gcgttggcgg tagcggtaat
    20701 tacgatttat gctaccttgg cagggaaagt tgaccacact gctgcgattt atattgcgat
    20761 gtggttgttg ggcggctttg tcattcaatt tgccgtagcg attattgaat taaaagaagg
    20821 ctctctgacc ggcgggaacg tattcttatt ctttgccggg atgttcatgt tgagcggggc
    20881 gatgaacgat gtgatcagtt ttctcgcggg tataaacggc ataaccttgc aaggtacgat
    20941 caacggatat ggatggctga ttttaaccat tgcccttacg acctggatgc ccgcatatct
    21001 gaagacagct ccggcggtat ttagtctgac cgtggtagat ttagtggtca tggcctggtg
    21061 tgttaccttt atcaagctgg gtttggccgg ggccattttg gttccgatcg ctgcctatac
    21121 ggcactgatt gccgctgtcg gggcgctgtg gtgcgccgct gccttcattt taaatcctgc
    21181 tttcggcaga gagattgtgt cactgggcgg taaattgtta aaatagaggc tatgaaaaaa
    21241 tgaacaccaa acataaatag acccgccgca atggcgggtt ttttttgcaa tgaccgcgaa
    21301 agtttatggg tcaggctggc agatcgtatg cagcggctgc atcccgtccg aagcaaaaaa
    21361 aatacccgcc cggagcgggt tttttagtga caatggtcgg ggcgacagga ttcgaacctg
    21421 cgactttctg gtcccaaacc agacgcgcta ccaagctgcg ccacgccccg aacctataaa
    21481 tgataacatt atttctgaaa tattgtcaat ttggattgtg acaacaaacg ttggctatgg
    21541 tcagctaatg ctcaaatcat gagcgatttg cattgccttg agcgtgtctt taccgacaat
    21601 gccggtggct ttaagttcct gcgctcgttt aaagcggata acggcctggc gggtgagcgg
    21661 accgaaccac ccgtcaacgc tggcggtata ttcgccgaga tcctgcagca gggactgcac
    21721 ctggcggacg tcatcacctt tgcagccctg ctttagggtg cgaatcttgc gcaccacgcc
    21781 gtcaatattg actggagtgc ccaccggaac ctggcggtag acgacgacaa catcttcatt
    21841 gtatagccgg atgcagccat ggctgaccgc tttgccaatt gatttgggat tgttggtgcc
    21901 atggataccg taactgcccc agggaacgct gagacgcatc catctggccc caaacggccc
    21961 gccgggattt accgatttga atacaatttt ccagtgcccg ggcggggtgg gagtgctggt
    22021 tttgcctaca gctaccgggt agacggtaag ggaatgctgg atgatcaggg tcaggatgcg
    22081 ggtgtcgacg tgtacgttga tgaccggccc ggaggtctga ctgactggcg ggacagcttt
    22141 cttcatcacc ttcctcctct cgctgcagcg ctttttatat tctattcgtc aacgtatagc
    22201 cgggtgattg cccggcgttt cagcttttta tgactgcttc gtcagggaaa acagtttttt
    22261 cttcgcagca agggggaaaa tagacgactt gcgtagaagc tatgcattaa aaagaaaaag
    22321 agatcactct ggtggggaag aaggggaagg gttgaaagca agcgaaaatg gcggttcccg
    22381 ctggcaaccg gtctggttgc tgatgattgt cagcttgttg gtcatgggat ttatggtttg
    22441 gaacgtaatg gttgtacaaa atggcatcct gaaacaggca gaaaaaattt atctccggga
    22501 acagacgcaa aatataacgg ctgttctggg cgaggtaggt cagcaggcgg aaaaaatggc
    22561 gatgcaaatc gccgctcaag atcagttgat gaaagcagtc gagcaaaacg acaacgatgt
    22621 cttggcagac aagctggtcc ctttgtatcg gcaatggaag caaaaccaag gcgtgactga
    22681 gctgagtctg gtttccgccg ccggggaggc ggtatggagt tcgctaaacg gggtcgaacc
    22741 cggaaatgac ttgagctatc agcgaaccac cagccaatcg ctgcaccagc agaaaagcgt
    22801 agctgcgatg gaggcggctg aaagggaaac catgctggta gtgacctggc cgctttttat
    22861 gcaggatgaa tacttgggat tgtgccgggt gggcatttct ttgtcttatc tgggcgaaca
    22921 gctgcagcag cggcaacccg ggcaagtggc tttgttcaat ctgaacggca ttgacagtca
    22981 gcttttatgg gagaatcaaa aaggatggtc agcgctcaat accaatgata tcaagaaact
    23041 gcaggacgga gagaccattt cccggccgtt tgactggtac acccgcttgc tcgtggtacc
    23101 gctgcaggac atcgaccgca tgaccgtggc ctatatccag aatcaactgt ccctgcaggg
    23161 atttgtccag gcccggctgt ttaattattt gatgttgctg ctggcgctgc tgctgatctg
    23221 cctgaccaat tatgcttatg gaagaagccg gtattatgcc gaagatgacc ttagcaacag
    23281 tcttgcccgg atatcgaaaa tcaacaatgt agctttccag gttcatcccg acaattcccg
    23341 gaagaacagc ccggatgaat gatgttcgga gcaggcggcc cggtgaggtt tgattaacag
    23401 gttgccaaga tcaaaaggta atattatcat aaaatttaga atatccaatc atttcaagat
    23461 tctatataat agaatcagag gtgaaggact ccactttgac acgtaacatg cataacgaac
    23521 atggaatttt gtatgtatta tgggggaagt aaaaaatgtc ttttttgccg cttaatgacc
    23581 cctgtccttg cggtagcgga aaagaatacg gacaatgctg tgcgccggga gccggatgcc
    23641 aggtgattca ttttccacgg ggaaaaagaa ataattttcg agcgattatt gaagaggcgc
    23701 tgggagactt gattctctat gcccgtcgtt actttcccaa ctgggatagt gtagcccagg
    23761 ccaaatttct gtcctattcc caggggggcg gagaaatcca tcagaaattt gcccacatct
    23821 tctggcagtg gtacgtttta aactatcgct tttatagtga cgtatcgccg ttgattgatt
    23881 tttatctggt ggaaatggag gatattctca ctgaaagaat gaaaacggtc tacgcggccc
    23941 tccaacattc ctaccttagt atttacacgg tcagttgggt ccggaacaac accgtggccg
    24001 tcaccgacct tttctgcgga gatgaacata ttatcgaacg ggactttggc tcggttaccc
    24061 agtttataga acaaggttcc ctcctgtatg gtcgcatcgt caagctggaa aatgcttcca
    24121 ttgtcatcgg gcgtccggtc atcgtcaccg ccgagcaaaa atcctatctt ttggacgaag
    24181 tcaacgccgt atatctgtcc gaaaacagcc gtacccggga agatatgcgt gaatttctgc
    24241 gtgagtgtgc ggaagtgaca gctggactgg tcatggatgt cgtccaggga attaagaaaa
    24301 accgagtcaa atccaagact ctgcacctgg aaagtcgtgc ccgcaaggcc ctgctgtaca
    24361 aattgaacaa cagccaacac tttgacatgc tggaaaggca caaccactgg ctcaagttta
    24421 cctggaaaga aggtcagggc atattcaagc ggctgtatgt aggcgaaaag gccctgctgg
    24481 tttcggcgga tgagagtgcc gatattctat atgccgcccg cttgctggaa gagatgctgg
    24541 ctgttgaaca aaccgagttt gtctggcagg acggcatcat cctcgatgac ggagaacagg
    24601 aagaagacat tcaaacggaa ttgatggtgg acaagaatct ggaagactgg ctgaaactgc
    24661 cgcatcccga actggctgac atgaccccgc tggaggccat gcaggatatc aaagggcggg
    24721 tgctgctgga aaacctgctc actgatatgg agatgctgga actgatcgcc cgcagccgcg
    24781 gggagtacaa ttatcccaca gccgtgatca gaagaactct gggactggat aaaaatgcgc
    24841 tcagccgtga gatgtccaat ccccaggcca ttgccattaa ggtggagaag ctgcgcaacc
    24901 gacaattgct gagttcctat gtgaccgcct acaactggct gagcagcgag tatgcccagg
    24961 tcgctgcaac aatttatgat atatacagca atggcgggat ggaccagaga agactggcct
    25021 ggctgctgta tttatggtgc gaattcacca ccgtgtaccg gccgcgcgta tcgcgtattc
    25081 acaactggat tgctgcgttg gaattcacct tgtccaacag tctgggtgaa gatatcagtt
    25141 ataccaaact atctcgtgct tttggcatct ctactggcat ggtcagccgc agcgcctata
    25201 tcgtcaaccg gcactttgaa aagtatccgc ccaatttcaa gaccgaaatg attcattatc
    25261 cctcatggga agaactggac cattatgaaa tggtgcaatc ctatgaggag gtctatcatc
    25321 acctcagcat atattcctat acaatcggca accgcgaccc gaaaaccaaa accgaggtac
    25381 aaactgctta ttatcagccg gtcaataccc gggcgcgctt ctgggatgaa ctgaacaaaa
    25441 aaatctacgg tgacttcttt gaacatcatt atttgctgga tcacataaac gcgaacggct
    25501 cgaccatcat gaacaccttt tgggacaatc aggccaaccg tttcccgccc tatctgcggg
    25561 aagccgcatt ccgtttcatg atgagctatg tcggtgccta ccgtatcaaa ccggcaggca
    25621 aaaacagcct gatctttgaa gatgtattca gcggggaaga gtttgaagtc tacggtcgtt
    25681 tcggtgacaa tgttcatgag aacatcgttc ccggcatgat aggcatctgt cgcctgctgc
    25741 cgttggagaa actgcgctgg gtcagcgacc cgatgttcat tgtgctgcag gacatgcagg
    25801 acatctttga gcgcaacctg gagatcatga ccgaaaattt agccggatac gatgccagcg
    25861 atccgctgta tctgaaaaag cgcggtgaat ttctggtcaa ggcctatatc cgctccatcg
    25921 atgaatttga gcaggccgcc ctcaatctgg tcaaccagcc actgcagccg gaatggcaat
    25981 atgcttatgt tagctgttat gaaaaagccc acgagctgat cagcagctgc aaacattttc
    26041 ggccgcttta ccaggaccac agccgcagca gttatatgtg ggatcggttt agtgtccagg
    26101 gcaactacca gtggggctat ctgttggtca aggacaacgc cattattatt tcggcgccgc
    26161 cgggcaagga catgcacaag tttaccaagg atatccgccg ggctttcaaa tgcgccgatc
    26221 tggtactggc cttccggccc gccgaactca gccgcaaagt gctgaaagag ctggaagttt
    26281 acatggtggc cgatctggcc aactattttg accaaaatcc cgcccaatcc ttaatcctgt
    26341 tgcggcagga tactttcaat aatgaagaaa aggaatggca gcaggggatt tttctgttga
    26401 aactgggctc actgctgatg gactacctgg aaaataaaaa aaagaatgag acgaaacaac
    26461 gttagaataa ctgcagcatt atacagggta ataattgtga ttggatcctg tttaactggg
    26521 aataatctta aggggcgcag ttaggaccag ggcagcagga attgggcaga aaatcaaatt
    26581 tatctgcttg aataccttga cttgcacagg ggttattgct tattcttatt gagaagaatt
    26641 gtttttgatg cggaagtggc tcagtggtag agcatcgcct tgccaaggcg agggtcgcga
    26701 gttcgaatct cgtcttccgc tccataagaa acaaatccct cggctttttg ccggggtgtt
    26761 gtgtttttag ccaaaataga gtacatttct ttaggaagcc tttcctttgg ttgcatgaca
    26821 gttttctcaa agatttggta atcatttgcg gcaaagaaaa gatttcaatt ttagttagga
    26881 ggagcataaa tgagtgccag gttagaaaag atagaaaaaa gtgaggccta tctgagcatc
    26941 gaggtggatg cgcagacttt tgaagagggt ttgcagaagg cctaccggaa agtcgttaaa
    27001 caagtaacca ttccgggatt ccgtaaaggc agagtgcccc gggaactgct ggaagcccat
    27061 tttggacgcg aaatcctttt tgaagatgcc atcgaaatga tcgttcccga agcctttgaa
    27121 caggcgctga cggaattaga tattgaaccc attgctcagc ctgagtttga aatagaagag
    27181 ataaaggaag acaatatttt gcggtttacc gccaaggttc cggttaaacc ggaagtcgta
    27241 ctgggtcaaa tcgaaggcct ggaagtcacc attcccatac tggaaatgga tgatgcagat
    27301 gtggaccggc gtatggaaga catggccaac cattatgccg aactggtgga aaaaaccgaa
    27361 gagcctgccg aaatgggtga tactgctacc atcgactttg aaggattcgt ggacggggtt
    27421 gcttttgaag gcggaaaagg cgaagactat ccgctggaat taggctctca atcttttatt
    27481 cccggtttcg aagaacaggt tgtgggatta aaagtcggtg acacagctga tgtaatggtg
    27541 atatttccgg aaacctatca ggcggaagaa ttggctggaa aaaatgcggt tttcaaagta
    27601 accgtacata ggatcgagac caaacagccc cgagagctga atgatgaatt tgcccaggaa
    27661 gtcagcaatt ttgagaccat tgccgagctg cgcgaagacg tgcaaaagag catgcaggaa
    27721 tccatggaca accgccgtct gggtttcatc cgcgatgcag tgattgccaa agccttggag
    27781 aacacccagg tcgatgtccc tggcgccatt gtcagaatgc agctgcaaaa catgctcaat
    27841 cagttcgctg aacagctgaa gatgcagggt ctgtccattg agcagtactt ccagctgacc
    27901 ggccgcaatg ttgaccggtt caatgaagaa atgtggccgg aaggcgagaa gaatgcccgt
    27961 gtcaatttca tgctggaaaa aatcactgaa gaaaaaggga ttgagctcag tgacgaagaa
    28021 ttagataatc atatcattga actgtccact tcaatgggaa ttgaccccga tcaggccaga
    28081 caaaatctag ccggcgtgat ggatgatttc atctatcggc tcaagatcca aaaggccatt
    28141 gattatttgg tggagcacgc ggttgtaacc gaaaccaaag acgacgaaga aggtgcagat
    28201 actgtccccg catcagctgt agaagtggaa ccggaagcct gaaattgaat actatagata
    28261 ttgtctgatg aatattaagt agaggaggga gttccaatgt catatctggt gcccatggtc
    28321 gtagaacaaa ccaatcgcgg agaaagatcc tatgatattt attcgcgttt gttaaaagat
    28381 cgtattgtgt ttttaggcag tgcgattgat gatacggtag ccaatttaat tattgcccaa
    28441 ctgcttttcc tggaagccga gaaccctgac aaagacatca acctgtacat caatagtccc
    28501 ggtggctcaa tcactgcggg aatggccata tttgatacca tgcaggtaat ccgtcccaac
    28561 gtttcaacca tttgcgtagg catggcggcc agcatgggag ctttcctgct tgccgccggt
    28621 gagcaaggca aacgttttgc cttgcccaat tcggaagtga tgattcatca gccgtccgga
    28681 ggcatggaag gccaggcgac ggatattgaa atccgcaccc ggcgtatttt acacatgaaa
    28741 gaaaccctca atcgtatttt ggcggaaaga accggacagg atctggaaaa aatccgtcgt
    28801 gatactgaaa gagattattt tatgaccgcg gctgaagcca aggaatatgg tctgatcgac
    28861 gagatattaa ctggtcccaa gaatagctaa ccactgagtc cgccgaaaag agaggtgaca
    28921 aaatgccaaa acatggtgat gaaaaaggcc aactgaaatg ttcgttttgc ggaaagaccc
    28981 aggaacaagt caagaaactg gtggcagggc caggtgtgta catttgtgat gaatgcatcg
    29041 agctttgtaa tgaaatcatc gaagaagaac tgtccgatga cattggtttt gaattggggg
    29101 aaattcccaa accgcaagat ataagagaaa tcatggatga ttatgtgatc ggtcaggata
    29161 ctgccaagaa atctttggcg gttgcggtat ataatcatta caagcgcatc aatctgggta
    29221 tgaaactgga tgatgtcgaa ctgcaaaaga gcaatatcat gatgctcgga ccgaccggca
    29281 gcgggaaaac cttgctggcg caaacgctgg cgcgtatttt gaatgtgcct tttgccattg
    29341 cggatgcgac ctccctgacg gaagcaggtt atgtgggtga agatgttgaa aacatcctgc
    29401 tcaagttgat ccaggcggct gattatgata tcgaaaaagc cgaaaaaggc attgtttata
    29461 ttgatgaaat tgacaagatt gcacgcaaga ctgacaatcc cagcattacc cgggatgttt
    29521 ccggcgaagg cgtgcagcag gcgctgttga aaatcctgga aggtacggtg gccagtgttc
    29581 cgcctcaggg tggacgcaag cacccccatc aggagttcat ccagattgat accagcaata
    29641 tcctgtttat ctgcgggggc gcttttgaag gcatcgacaa gatcattcag agccgcattg
    29701 acgaaaaagt gatggggttt ggcgctgata tcaaaaggca caacgaaaag aaaatcggcg
    29761 acattttaaa tcaaatcctg ccgcaggact tgctgcgcta tggcttgatt cctgagtttg
    29821 tgggacgtgt tcccatcatc gttaccctgg atgcgctgga caaagacgcg ctggtgcaga
    29881 ttctgaccga acccaagaac gctctggtca aacagtataa gaaactgttt gagctggatg
    29941 gtgtggatct ggagtttatg gaggattccc tgctggccat cgctgacgaa gccttgcgcc
    30001 gcaacaccgg ggcccggggc ttgcgagcca ttatcgaaga cgtcatgctg gaggtcatgt
    30061 atgatgtgcc ttcccgtctg gatgtgacca aggtgctgat caccaaggac gtgatcgaaa
    30121 agaaggaaaa acctctgctg gtgacagtag acgccaaacg caaagtaaat tagagcgggg
    30181 caaaaaacaa ataactgcat tatataaaaa acaccggcct tgtgacaagc cttgggcgca
    30241 gtcgcaaggc ttggttgcgt ttatgctgaa agtgcaaaaa gcccgttcag ccgacgtaca
    30301 tgttaagggg acattcttct gaaggagtgt gtccccattc cttataccgc ctcattcacg
    30361 ggctttttgt cataccttgc ttttcggctt tttgactggt ctgtttaaat tgaacttcat
    30421 taatcgctct ggaaaaggca gtgtaagctg ccttttttta cactttcctt ccctgttggc
    30481 aaaagatgat tgacgggcat aatagaatat aatgaaccag gaaaggaaat caaccatgaa
    30541 cagctttttt atgcaatacg ggggcattct ttatctggtc cagttgtttt ttgcggtcgt
    30601 gattggtctg tatttctgga atctcttgaa aactcaacaa aaaggcaaaa cggtcatggt
    30661 gcacgagtcc gaaaaagaaa gaaaccggtt gcgggcaatg cgcagcatca agctgaccga
    30721 gcccctgtcc agtttgagcc gtcccggttc atttcaagac atcgtcggcc aggaggaggg
    30781 tatcagcatc ctgcgctcgg ccttgtgcgg ccccaatccc caacacgtaa ttatcttcgg
    30841 gcctcctggc gtaggcaaga cagcagcagc ccgcctggtt ttggaagaag cccgtcaaaa
    30901 cgcactgtcc cccttcaacc ccaatgccag ctttgtggaa atggatgcca ctatctgccg
    30961 cttcgacgaa cgcaatattg ccgacccact gattggctcg gtgcatgatc cgatttatca
    31021 gggggccggg gccatgggta cggc
//
LOCUS       DAOTHZ010000019        30423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1689_length_30423_cov_8.162869, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000019 DAOTHZ010000000
VERSION     DAOTHZ010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 30423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30423
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1689_length_30423_cov_8.162869"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..174)
                     /locus_tag="P5549_03845"
     tRNA            complement(82..174)
                     /locus_tag="P5549_03845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(138..140),aa:Ser,seq:gct)
     gene            complement(184..271)
                     /locus_tag="P5549_03850"
     tRNA            complement(184..271)
                     /locus_tag="P5549_03850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Ser,seq:tga)
     gene            complement(418..1692)
                     /gene="serS"
                     /locus_tag="P5549_03855"
     CDS             complement(418..1692)
                     /gene="serS"
                     /locus_tag="P5549_03855"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRW12235.1"
                     /translation="MLDAKMIRANHEKVEQAMRSRNMPGALDNFLRLDEERRKLLTQV
                     EAVKSYRNKASQEVGELKKKGQNADELMEKVRSVSQEIKELDDQIKLVENDLDKILLN
                     IPNIPHESVPVGKDENSNLEMRRWGEPRQFDFEPLAHWDIGPGLDVLDFERAAKLSGA
                     RFTVYKGLGARLERAVINFYLDTHTMHHGYREIFPPFMVNAECMLGTGQLPKFADDMF
                     KVEGRDMYLVPTAEVPLTNLYREEIIDGRELPFYLTAYTACFRAEAGSHGRDTRGIIR
                     QHQFNKVELVKIVDAESSYEELEKLTADAEKVLQLLELPYRVVLLSTGDMGFSSAKTY
                     DLEVWMPSYNEYKEISSCSNCEDFQARRANIRYRPADGSKVRYAHTLNGSGVAVGRTV
                     AAIMENYQQPDGSVVVPKVLRPYMGGLGVIQK"
     gene            complement(1725..3317)
                     /gene="serA"
                     /locus_tag="P5549_03860"
     CDS             complement(1725..3317)
                     /gene="serA"
                     /locus_tag="P5549_03860"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRW12236.1"
                     /translation="MEKKKIIVSERIAEEGVAILREKAEVDFRDGISREELLSIIDQY
                     DALIVRSITQVNEELIQRATRLKVVGRAGNGIDNIDVEACTRYGIIVANTPDSNTISA
                     AEQTISLLLSSVRNTAWANDVLKGGTWDRKPFRGTELYGKTVGIVGLGRIGSMVATRL
                     KAFNMKVIAYDPYISDERFEKYGAEKKETLEELAREADIITVHTPRTEETTHIIDEKV
                     LSYAKEGVMLVNCARGGIMKEAALLEGLKSGKVRSVGLDVYEKEPAIGNPLFDFDRVV
                     CTPHLGADTFEAQKRVGETIAEQVLLALEGGIVPNLVNLPSMLSDDLNYLRPYISLAE
                     KMGKIYYQLHKSPLSRVEITYSGPFTSNDTEILTIGFLKGLLEPVLWGKVNYVNARLM
                     AEERGIKIFEAKEEQSARRYKNIITMKFFSPNHELEICGTISRARNPLLVEIDGYELE
                     ADLDGYVLMLQNEDRPGIIGQLTMAMGDAGVNIAFMRVARKTKGETAIMVLNVDNKVE
                     DEVIQKLTTINGIVGTPKLLHF"
     gene            complement(3342..4502)
                     /locus_tag="P5549_03865"
     CDS             complement(3342..4502)
                     /locus_tag="P5549_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRW12237.1"
                     /translation="MKGEPYLFLPGPTPVPERVCRAMNRPMINHRGDEFKDILEDVVA
                     GIKQIYRTRANVLIYPASGTGGLEAAVVNFISPGDQVLAVSIGVFGDRFAMIASNFGA
                     VVEKMDFSWGTGADPQKIKERLAQDTDHQIKAVLITQNETSTGAYNDVKAVREAMGDH
                     PALLMVDAVSGLAAIELRMDEWKLDVVISGSQKAFMIPPGLSFMAFSDRAYQVHKSNT
                     NTSYYWNVDQGMEYQLKGQTPFTPPISLYFGLHEALQMMAEEGLDNVIARHTAYRDLV
                     RASLRALGLKLLTEDRYASAAVTSVIAPPEIGANQIRKYMFDQFNIILAGGQQSLDNV
                     VFRVGHLGYIRELDLLSVLAALEITLLHFKFPLELGTGLKIAQKAIFDMHQQ"
     misc_binding    complement(4544..4745)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4796..5368)
                     /gene="pdxT"
                     /locus_tag="P5549_03870"
     CDS             complement(4796..5368)
                     /gene="pdxT"
                     /locus_tag="P5549_03870"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRW12238.1"
                     /translation="MAGKKIGILALQGAFIEHQQALQRCGAQPVQVRSIKQLEQVDGL
                     IIPGGESTTIGKLMQAYQMDTAIRNRAEQGMAIWGTCAGMILLAREIIGMEQPHLGLM
                     DIAVIRNGFGRQVDSFETELEIDRIGPFRGVFIRAPYVEKAWGEARVMARHSDKIVMA
                     EDDRLLVTAFHPELTKDDGIHEYFLKKVAK"
     gene            complement(5358..6242)
                     /gene="pdxS"
                     /locus_tag="P5549_03875"
     CDS             complement(5358..6242)
                     /gene="pdxS"
                     /locus_tag="P5549_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRW12239.1"
                     /translation="MVEKGTFTLKSGLAQMLKGGVIMDVTTPEQAKIAEAAGACAVMA
                     LERVPADIRATGGIARMADPTIIMKIMEAVTIPVMAKCRIGHFVEAQVLESLGVDYID
                     ESEVLTPADDKYHVNKHDFKVPFVCGARNLGEALRRIGEGAAMIRTKGEPGTGNVIEA
                     VRHIRQVNDEIRWISHLPPEELMTAAKELQAPYELVQEVARQKALPVVNFAAGGIATP
                     ADAAMMMQLGCDGIFVGSGIFKSGDPAKRAKAIVAATTYYNDAAVLAEVSRDLGEAMV
                     GINIDTIKPEERMQDRGW"
     gene            complement(6272..8704)
                     /gene="gyrA"
                     /locus_tag="P5549_03880"
     CDS             complement(6272..8704)
                     /gene="gyrA"
                     /locus_tag="P5549_03880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRW12240.1"
                     /translation="MGQENLFNKIIPIDIEKEVRKSFLEYSMSVIVSRALPDVRDGLK
                     PVHRRILYALYDQGMTHDKPHKKSATIVGEVMGKYHPHGDAAIYQTMVKLAQDFSMRY
                     PLVDGHGNFGSIDGDSAAAMRYTESRMSKIANEMVKDLDKETVDWRPNYDDTRKEPGV
                     LPARIPNLLINGTSGIAVGMATNMPPHNLSEVVDALNLYIENPDVTLDELMKVLPGPD
                     FPTSGIMSAAGIRQAYESGRGSIKVRARYEIEERKGGKTAIIITEIPYQVNKARLVER
                     IAELVRDKRIDGIVDLRDESARVGMRIVIEVRRDVNVNVLINKLFKHTQMEDSFGINN
                     LALVNGEPKTLSLKDMLKYYIEHRQDVITRRTIHDLKLAQARLHIVEGLQIAIDNIDE
                     IMNLIRSSKDRETARNKLMESFALSDIQANAILDMRFAQLTGLERAKLEDEHQELLAR
                     IADFEDILANPARVLAIIKEDLDDIKAKYGDKRRTEITYDNVEYDEEDFIEDHEVVIT
                     LSNRGYIKRQPLDTYKAQKRGGKGISSNIRAEDFALDILVTGVLNNILFFTNQGRVFS
                     TKAYRIQEASRQAKGQALVNIMDLRPDEKVSTLVAVKIFDDHRHLMMVTRRGIVKKVP
                     LNAFSNIRKTGLIAINLGEGDELVGVLRVDGDDQVMLVTNYGQSISFDEKQVRPMGRQ
                     AAGVKGINLGKNDFVVGVDKYRTNGEAVLVTLNGYGKRTRLEEFNLQHRGGKGLKAIE
                     IGKKNGPVVGFKVVKEDEEIIILTSEGHIIRLETEDISIQKRYSRGVVLMRIAQDDSI
                     AAVARFKSEKEE"
     gene            complement(8745..10667)
                     /gene="gyrB"
                     /locus_tag="P5549_03885"
     CDS             complement(8745..10667)
                     /gene="gyrB"
                     /locus_tag="P5549_03885"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRW12241.1"
                     /translation="MTNGNGNINGDQAYNASDIQVLEGLEAVRKRPGMYIGSTGPKGL
                     HHLVFEIVDNSIDEAVAGYCDKIELIIHPNNSITVKDNGRGIPVDTHPIMGIPAIEVI
                     LTTLHSGGKFGGSGYTISGGLHGVGLSVVNALSSLMVIKVKRDGKIYSQQYMRGKPAG
                     PLEIIGESTETGTSIYFEPDEEVFEEIVFEREILVQRIRELSFLNKGLLIEVIDERDP
                     EGYQKAFKADGLHDFVAYINKNKEPLHDKEIYFDVKKEDVVVEIAIQYNNGYAENIYS
                     FANNIRTQEGGTHEVGFKNALTRVINDYAKKNGILKESEANLNGEDIREGIVAIISVL
                     VKEPQFEGQTKTKLGNTFVRSVVESTVYEKIDDFLDENPADAKRILEKAISARRAREA
                     AKKARELTRRKSALESTSLPGKLADCSSKDPAVSELFIVEGDSAGGSAKQGRDRNTQA
                     ILPLRGKILNVEKARLDRILASEEIRNLITAIGTGIGEDFDISKARYHKLFIMTDADV
                     DGSHIRILLLTFFYRFMRELIEHGYVYIAQPPLYGVKRGNEIHYFYNDNELNKFMSQT
                     DKKWALQRYKGLGEMDAEQLWHTTMDEDNRVILQVTIEDAIQADEVFSDLMGDNVEPR
                     KEFIQTNAKYVSNLDI"
     gene            complement(10683..10922)
                     /locus_tag="P5549_03890"
     CDS             complement(10683..10922)
                     /locus_tag="P5549_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRW12242.1"
                     /translation="MFIHLGNDILIPAQSLIAIINIKDPWSEDIQDVLDLAEMEHKLV
                     NVSAQEKKKALVICDDRVYFSPISSQTLLKRANIF"
     gene            complement(10925..12022)
                     /gene="recF"
                     /locus_tag="P5549_03895"
     CDS             complement(10925..12022)
                     /gene="recF"
                     /locus_tag="P5549_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRW12243.1"
                     /translation="MRISQLSINNFRNLKNINFTPDQAINIFCGTNAQGKTNLLEAIY
                     VLSAGSSFRASNDRQLINYDAERYQILAAYNYQERELTSSLSYINNSKIFTINHKKTS
                     THNQHRLKVVLFNPDDLFLIKGSPAKRRALIDFLLQQLSAEYTTALANYVKILKKRNL
                     MLKKEQTSQQSFAIIDQLFIESAVNVILQRINFINRLDEEASFLFKEINDAGNQLKLR
                     YALSFPVESDKINLNILSGALLAHIKNIFPEENKRRQSLTGPHVDDINFYLDNRPARI
                     YASQGQQRNIVICLKLAEMYTFKRIKGYFPVFLLDEVLAELDEEKRNRLLEYLQKAEF
                     QSFLSSVNFNPPDDFKASLYTIANGELLGKE"
     gene            complement(12041..13180)
                     /gene="dnaN"
                     /locus_tag="P5549_03900"
     CDS             complement(12041..13180)
                     /gene="dnaN"
                     /locus_tag="P5549_03900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRW12244.1"
                     /translation="MKMNIEKRELSALTTMVYRAASNKNTIPVLSGLLLELSPEKGLT
                     MTATDMEIGICASTSNVDVIEPGTVLVNAGYFADFIKLLPDGNIAMEVNVSTSKLNIH
                     YGRSKGNINIYREQDYPALPIDKMEYKFSMAQNVLKEALRKTVFAAAANHFRQVFTGV
                     LFDLQNNNQLRVVASDTHRLAYYTHVLNETLAEPFKFVIPIRTVNELLRFLEDSDEEI
                     KIALSDNNVIFYKEGFLFLSRLIEGQYPNYEQVIPASFVTRLDIQTHVLATSLERVKI
                     MPTDDKLKIPHLQLSFENEEVHIFSNSEMMGEINEVIEGVGIEGDKEIKIVFNTHYFL
                     DAVKIFDQECENLSISLSGSFGPAMLKNNEKDNYLYILVPMRTSN"
     gene            complement(13326..14633)
                     /gene="dnaA"
                     /locus_tag="P5549_03905"
     CDS             complement(13326..14633)
                     /gene="dnaA"
                     /locus_tag="P5549_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRW12245.1"
                     /translation="MSTNQDLVAVWEKVLDAVRQEIGSTSFDIWFSKVKYNTSRDEKI
                     YITVPNTLTKEWIESRYLENMQKYFRALSSPDISLILTTESIKKNTYISPFNPKYTFD
                     TFVVGNSNRFAHAACYAVGESPSRAYNPLFIYGGVGLGKTHLMQAIGHRIVKNYPKYS
                     VMYVSSEQFTNELIGSIKDDNTSGFRNKYRNIDVLLIDDIQFLAGKERTQEEFFHTFN
                     TLYESNKQLVISSDRPPRNIPTLEDRLRSRFEWGLITDIQTPDLETRVAILRKKAQND
                     NFNIPYDVIDYIANYIDSNIRELEGALVKLVAFATINKLPLDMNTATIALKDILPPPG
                     PKKITIECIQKEVCKFYGLELSEMLSKKRNKNLVLPRQIAMFICRRMTDASLPLIGDQ
                     FGGRDHTTVIHSVEKITREMEEQAELYTSVKDICKKIDPDFAE"
     gene            14905..15042
                     /gene="rpmH"
                     /locus_tag="P5549_03910"
     CDS             14905..15042
                     /gene="rpmH"
                     /locus_tag="P5549_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRW12246.1"
                     /translation="MVKMTYQPKKRQRKKVHGFRKRMATASGRKVLATRRKKGRKVLS
                     A"
     gene            15214..15549
                     /gene="rnpA"
                     /locus_tag="P5549_03915"
     CDS             15214..15549
                     /gene="rnpA"
                     /locus_tag="P5549_03915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRW12247.1"
                     /translation="MLQKKYRISNRADYNFIYQNGKKIHCRYIIVFITANQLAYNRFG
                     IVTSKKVGNAVHRNQARRRLRAIIQKHMPEIKQGYDLVMVARFNIKEAMFAALEKDFL
                     IAAKKAGLI"
     gene            15546..15752
                     /gene="yidD"
                     /locus_tag="P5549_03920"
     CDS             15546..15752
                     /gene="yidD"
                     /locus_tag="P5549_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRW12248.1"
                     /translation="MSKVFIFFIKLYQKGSFLKRPSCRFYPTCSQYAVEAITKYGAAK
                     GGWLALRRISRCHPFHPGGYDPVP"
     gene            15835..16689
                     /locus_tag="P5549_03925"
     CDS             15835..16689
                     /locus_tag="P5549_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRW12249.1"
                     /translation="MWSSFVQWFAGVIDFTYSLTVQMGLPSYGLAIILMTIAIKILMF
                     PLTQKQMHSMAAMQEIQPKTKYIQEKYKDDPQVMQQKIMELYKEHGVSPFGGCMPLLI
                     QMPIFIAFYQALFNFKFVNLAHAGFLWIPNIGKPDPYFLLAILAAATTYYQQKISMVD
                     SKDPTQKTMLYFMPIFMAFIAYKLPAGLPLYWVVFNILGILQQLYVNSQKRKVAASTG
                     TASLVMETQDNVKPSAAAKADSKGDSAQKQQAAKASEGGNTKDAAKDKGGKAKNASPN
                     SRKKGKNH"
     gene            16649..17281
                     /gene="jag"
                     /locus_tag="P5549_03930"
     CDS             16649..17281
                     /gene="jag"
                     /locus_tag="P5549_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRW12250.1"
                     /translation="MQVQTVEKKGKTIDDAIQAALEELGCGLDEVEVEILEEGSKGLL
                     GLIGSKPAAVKVSRKVDNPAEDARQLVEELLTRMKIDHEIEAVTAEQGTVRINIIGKD
                     MGLLIGRKGETLNSLQYIAGLMINRKRGERIRLILDVEDYRKKRDESLESLAVRLSEK
                     VKATQKNVVMRPMSSQERRIVHTALQEDPQVVTYSMGDEPNRKVVIALKK"
     gene            17411..18790
                     /gene="mnmE"
                     /locus_tag="P5549_03935"
     CDS             17411..18790
                     /gene="mnmE"
                     /locus_tag="P5549_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRW12251.1"
                     /translation="MNNDIIAAVSTPPGEGAVGIIRLSGHGAIEIAEKTFLPYNKNVS
                     LRERRNFSLTLGWILDAEGIRTDEVLVGIMRAPHSYTGEDVVEINCHGGGAALRACLQ
                     RCLETGARLAEPGEFTKRAFLNERLGLEQAEAVLEIIRAKTERGLQLAVRQLSGYNGR
                     FMDNLEERLLRLNAMVDASLDFPEEVGDLDQDEARQLLDGIIATIDDWINSARRAEIY
                     RDGIELAIVGKPNVGKSSLLNALSRKEKAIVTDIPGTTRDVVEEYINVRGIPVKIMDT
                     AGIRATEDRVERIGVEKSRELIDQADLVVLVLDFAAGIAAEDLEIFQTIPREKCLVLV
                     NKEDIERKAITEEELAEKFASARLIRASVKEDYGLDELERAIEELLLSGTQNADGMEI
                     MINLRQKNALLKSRAYAENAREQLGRISLDCLGVDVWGALESLGEISGKSLKEEVLDR
                     IFHDFCIGK"
     gene            18812..20689
                     /gene="mnmG"
                     /locus_tag="P5549_03940"
     CDS             18812..20689
                     /gene="mnmG"
                     /locus_tag="P5549_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRW12252.1"
                     /translation="MIYEAGKYDVVVIGAGHAGCEAALAAARMGVSTLLVTLNLDAIA
                     LMPCNPSLGGPAKAQLVREIDALGGEMAVNIDKTNIQIRMINTGKGPAVQSLRAQADK
                     QEYQKEMRRTIFQQAGLDLLMAEIIAIETVDGRVCAVVTRTGARFRCDAVVVTSGTYL
                     KGRIIIGDLMYDGGPGNQYPAAELSKSLLSLGIQLGRFKTGTPPRVDRKSVDFSKTIE
                     QPGDPDVPSFSFISDLAPREQLSCWLTHSTTETHRIVMDNIERAPLFTGVIKGKGPRY
                     CPSFEDKVVRFAHKESHQLFIEPEGRATDEMYVQGLNTSLPEDVQLEVLRSIPGMENV
                     RILRTGYAIEYDYVIPSQLKLSLESKVVEALFTAGQINGTSGYEEAAAQGLIAGINAA
                     CKVQNKPPFVLKRSEAYIGVLIDDLVTKEADEPYRLLTSRAEYRLLLRQDNADLRLTE
                     KGREIGLVSDQRWAKFMDKKRRLEEMRSLLEKTTFTPADRIITAYLEEIGSAMLRDRV
                     SLWELLRRPEVTIDDLLTRSLVPEMKKEISEQLEIQSKYEGYIQKQQEQVKRFEKLES
                     KAIPADMEYQNIKTLSSEAMQKLEQFKPETIGQASRISGVTPADVNILLIFLKKREGI
                     K"
     gene            20695..21342
                     /gene="rsmG"
                     /locus_tag="P5549_03945"
     CDS             20695..21342
                     /gene="rsmG"
                     /locus_tag="P5549_03945"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRW12253.1"
                     /translation="MEIYVNRFIDFLFAENEKQNLISRQAGREDVLNHIEDSLAILQF
                     ISLAGKRVIDIGSGAGFPAIILAMHCEDAQFVLVESDLKKSSFLESCRLQLNLKNVQV
                     VRARAEEIGHDKQYRAQFDCCSSRAVAAMNILLEYGLPLLKTGGKMLLWKGLNYQAEL
                     ETAQHALQVLRAGVEGICPYTLPGDRQRIIVVVNKQAETSPQYPRRVGIPAKRPL"
     gene            21401..22264
                     /gene="noc"
                     /locus_tag="P5549_03950"
     CDS             21401..22264
                     /gene="noc"
                     /locus_tag="P5549_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HRW12254.1"
                     /translation="MEVKKQLEKILHVGSGSRVLELPLEQISPNPFQPRKEFDELELK
                     ELAKSISSFGVIQPIIVRKSKQGFQLVAGERRYRACKLIGRQQIPAIVQDIDDQSMAA
                     ISLIENLQRKELNYFEEAIAYSTLLNRFGLTQEELAQRVGKSQSAIANKLRLLKIAPE
                     IRNLIITTTITERHARALLKLNTIEMQKEIIRAIYERELTVKETEELVEKAQRNNIPR
                     EPKTSNNGQQISMVIRDARIFLNTIKETVNRAKQTGIDMRVFENENENEYEITIKVKK
                     LRKNSRLMAEI"
     gene            22601..23383
                     /locus_tag="P5549_03955"
     CDS             22601..23383
                     /locus_tag="P5549_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRW12255.1"
                     /translation="MAKIIAVANQKGGVGKTTTSVNLSAYLALEGYRVLLVDCDPQGN
                     ASSGLGINRKKIPYCVYNLLIDGMEIKKVLCQTRINNLVILPATIQLAGAEVELANRP
                     GREFILSQALQPARDEYDYIILDCPPSLGLLTINALTAGTSVLIPLQCEYYALEGLSQ
                     LMDTIQLVKSRLNPSLRVEGILFTMFDGRTNLAIQVVDEVKRHFHKEVYQTVIPRNVR
                     LSEAPSHGKPIALYDPRSKGAEVYRDLAREVIERGKTRKRTR"
     gene            23355..24194
                     /locus_tag="P5549_03960"
     CDS             23355..24194
                     /locus_tag="P5549_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRW12256.1"
                     /translation="MAKPERGLGRGLDALFSVEESIDEKLVAEIEMKLIRPRKNQPRK
                     NFDPERLQELAESIKERGVLQPILLRPREKGYEIVAGERRWRASKMAGLKMIPALVRE
                     MSDAEVAEISLIENLQRDDLSIIEEALAYKNMIEEFDYRQDVLSQKIGKSRVHISNTM
                     RLLSLPQEVISLLENKQLTPGHARALLRLDSDSAQIKAARRIAEGKLSVRKAEQIIRF
                     DQKEARPAQNNTAEEMDLQERLQKHLGSKVEIKRRRQGGSIEITYYDHEDLERILEIM
                     GLK"
     gene            24279..24713
                     /locus_tag="P5549_03965"
     CDS             24279..24713
                     /locus_tag="P5549_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRW12257.1"
                     /translation="MHKKRENRTVESLADPAGGSGNSGLSTFIGGNRIFGYCLADRTS
                     LAASTSSAWLALAYLIIFGSVIAFTSFISALQLLPINIAMTYAFVNPVLAPVLAQILG
                     GWVLGEPLTLLGTTMVILGVVSVFANRNQADIKEKSPFHRAA"
     gene            25105..25827
                     /locus_tag="P5549_03970"
     CDS             25105..25827
                     /locus_tag="P5549_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12258.1"
                     /translation="MKKKPPSKIRKTGIKTIKGKKAPIYINVNMKNSSLGTITNCIQP
                     TNHPLLDREILKPSACQTWKTVCSAPGYNCVPRQGERVINGGFENHPDPFLGWVINAG
                     VGLIKPWQGDLPHQGRNAVCLGNEHGCALLYQDISGICPGMYLQLSYFLSAGSACGND
                     EVFIIVEFLDRCKKSIDGPVLEILVPCNSLSNETYTGFLNATRRGAPPGTKFVRIRFA
                     VDGNCAGQSRPIHLDDVSLIAI"
     gene            25981..26586
                     /locus_tag="P5549_03975"
     CDS             25981..26586
                     /locus_tag="P5549_03975"
                     /inference="COORDINATES: protein motif:HMM:NF026229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4489 domain-containing protein"
                     /protein_id="HRW12259.1"
                     /translation="MNKGKEKCCYDEEVIYEPEEMECKPHIPRHGETEVILHCGTVTG
                     SGPLSDYVNGGCGPAATVQASVVLDTERLRKPTVKIDFSALISYKTTYEDYFLHLGFR
                     LSKICGGATIPLGTWVFEKIHGPNFNPGNSAAERPPLPPNCDVFQETDPFFFSWCACG
                     DCPDCCRYIVELIDQQYYNVEYAVVTNIALTAMAVGEKRNV"
     gene            complement(26855..28033)
                     /gene="larC"
                     /locus_tag="P5549_03980"
     CDS             complement(26855..28033)
                     /gene="larC"
                     /locus_tag="P5549_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRW12260.1"
                     /translation="MHFVYLDCFSGISGDMLIGALLDGGASLTALQEGIEALNLGARL
                     AAAKQVRQGISCTTFRVDCPDAPPLRHLEQIEAILQTSSLPEAIKKDSLAVFRRLAQA
                     EAEVHGIDMSRVHFHEIGAVDTIVDVVGTFLCLADLGAEQVYASALPWANGVLDMSHG
                     RYPLPAPAVVRLLQGYPCVSSAAGIELVTPTGAALLTHLVKNQAAPNAFVPLAVGYGA
                     GSREREDKVPNLLRMIRAQADERLGQRETIAVLETEVDDLNPEIFTHLYRLCLDHPGV
                     MDFFTTPVYMKKNRPGTLITVLTARTAADEICRLLMRETGTLGVRYRFQERFAWPRHQ
                     ELLSTPWGQVRIKVARADDGTVYKKAEFEDCQAIARQNQLSLREVFAVVQNRLNDIPD
                     "
     gene            complement(28215..28826)
                     /gene="yyaC"
                     /locus_tag="P5549_03985"
     CDS             complement(28215..28826)
                     /gene="yyaC"
                     /locus_tag="P5549_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HRW12261.1"
                     /translation="MSIFTSDSSDPHSFHFEDPACFGHLQKIVYTHLINMNPGFEKDI
                     IYICIGTDRATGDCLGPMVGTRLQSTSLHPYVYGTLEQPVHATNLTQFTEQITRRYDN
                     PLTVAIDASLGNSSRIGFINVKKGGLKPGTALHKDLPEVGDFHISGVVNVGGYLEQMV
                     LQNTRLFLVYRMAEVIARSLLVAHLHFENEKNQRLPYANQANL"
     gene            28918..29970
                     /locus_tag="P5549_03990"
     CDS             28918..29970
                     /locus_tag="P5549_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12262.1"
                     /translation="MTGIKKQLVITMGYLGAVIGAGFASGQEIVQFFVCYGKWGAAGA
                     VLATVLFAVCGGMLLSMAHRQKIANYQAMLSIMFGPRLGKIVDFLLAIFLFLGLSTML
                     SASGAVFYEHLYLPKYAGILLAYVAVVIALAAGKKSLVFSYNVLVPVKIILLLMISGY
                     IALFAGEGQSSVYTSYLIPRGENAWLLSSLLYVAYNFSLAMVVLTAYQSVSNRREGIG
                     GAVLGGLVLGGLVLLNYAALSKYLPAVIHYQVPMLYIAGQVSPLAKSVYTLVLWIGIL
                     TTAIANAYGFAQRFARFTGIDYAVCLIVCMTLALPIATQSFSSLVNRVYPLFGLLGLL
                     ILSGLVYHFLRSRAGN"
     gene            30108..>30423
                     /locus_tag="P5549_03995"
     CDS             30108..>30423
                     /locus_tag="P5549_03995"
                     /inference="COORDINATES: protein motif:HMM:NF023807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRW12263.1"
                     /translation="MTNLRNIPSIDELLTQPETRHLLAVYQREFIVDTLRQAVNEVRT
                     ELRTSRQTFAKAEISRLVLQKAEVSLKNTVRGSLNRVINATGVVLHTNLGRAPLADRA
                     FQY"
BASE COUNT         7786 a         7161 c         7615 g         7861 t
ORIGIN      
        1 cgacctccag gttcgtagcc tgtcactcta tccagctgag ctacgggcgc aagtatttga
       61 aacgcattag caaatagtaa atggcggaga gggtgggatt tgaacccacg aagcagtttt
      121 aagccgctta ctcgcttagc aggcgagcgc cttcggccac tcggccacct ctccagataa
      181 ctatggcgga ggaggtagga ttcgaaccca cggagccgtg aagctcaacg gttttcaaga
      241 ccgcctcctt aaaccactcg gacactcctc cactgagaag gatgcgcatt tcgcatactc
      301 ctgcccaatg caaatgaaag tatatcacag cccatccccg tttgtcaacc tgctgtatta
      361 tggcttttgg gggttttgcc gacctttttt gcgggccttt tcatcagccc tgggcggtta
      421 tttttggatg actcccagac cgcccatgta gggtctgagt acttttggta ccaccactga
      481 tccatcgggc tgctgatagt tctccatgat cgccgccacg gtcctcccta cggctacccc
      541 tgatccgttc agggtatggg catagcgtac tttgctgccg tctgcgggac ggtagcggat
      601 gttggcccgc cgcgcctgaa aatcttcgca gttggagcag gaagagattt ccttgtattc
      661 gttatagctg ggcatccaca cttccagatc ataggtcttg gcagaggaaa agcccatgtc
      721 gccggtgctc agcagtacga cccggtaagg caattcgagc agttgcagga ccttttctgc
      781 gtcagcggtg agcttttcca gttcctcata ggagctctcc gcatcgacta ttttaaccag
      841 ttccactttg ttgaactggt gctggcggat gatgccgcgg gtgtcgcgtc cgtgggagcc
      901 ggcctcggcc cggaaacagg cggtatacgc ggttaaataa aagggcagct cgcgtccatc
      961 gatgatctct tcccggtaga ggttggtcag cggcacttcg gcggtgggaa ccagatacat
     1021 gtcccggcct tccaccttga acatgtcgtc agcaaacttg ggcaactgcc cggtccccag
     1081 catacattcg gcattgacca taaatggggg gaagatctct cgatagccat ggtgcatggt
     1141 gtgggtgtca aggtaaaagt taataaccgc tctttccagt ctggccccca ggcctttgta
     1201 tacggtgaat ctggctccgg agagcttggc ggcccgttca aaatcgagca catcaagacc
     1261 cgggccgatg tcccagtggg ccagcggctc aaagtcaaac tggcgcggct ctccccagcg
     1321 tctcatctct agattgctgt tctcgtcttt ccctaccggt accgactcat gcgggatgtt
     1381 ggggatattg agcagaattt tgtccaaatc gttttccacc agcttgatct ggtcatccag
     1441 ctctttgatc tcctggctga cggagcggac tttttccatc agttcatcgg cattctggcc
     1501 ctttttcttt agctccccta cttcctggga ggctttgttg cgatagcttt tgaccgcttc
     1561 cacctgggtg agcagtttgc gtctctcttc gtccaggcgc agaaagttgt ccagtgctcc
     1621 gggcatattg cggctgcgca tggcttgctc aactttttcg tgattggctc tgatcatttt
     1681 ggcatccagc ataattcttc tccttccggc cccagctgcc gggcttagaa atgcagcagc
     1741 ttgggcgtgc ctactatacc gttgattgtg gtcagcttct gaatcacttc atcttccacc
     1801 ttgttgtcaa cattaagaac catgatggcg gtttcacctt tcgtcttgcg ggcaactcgc
     1861 ataaaggcaa tattgacgcc ggcatcaccc atagccatgg tcagttgacc gataatgccg
     1921 gggcggtctt cattctgcag catcaagaca tagccatcca gatcggcttc cagttcatac
     1981 ccgtcaatct ctaccagaag cggatttctc gcccgggaaa tggtgccgca gatttccagc
     2041 tcatgattgg gactgaagaa cttcatggtg atgatatttt tgtatcttct ggcactctgt
     2101 tcttccttgg cttcaaagat ttttatgccg cgctcctcgg ccatcaggcg ggcattgacg
     2161 taattgactt ttccccataa gaccggctcc agcagccctt tcaggaaacc aatggtcaat
     2221 atttcggtgt cattgctggt aaatggacca ctataggtaa tttctactcg gctgagcggg
     2281 cttttatgca gttgatagta gattttgccc atcttctctg ccaggctgat ataagggcgc
     2341 agatagttga gatcatcgct gagcatggaa ggcaggttaa ccaaattggg aacgatacct
     2401 ccttcaagtg ccagcagcac ttgttcggca atggtttcgc cgacacgttt ctgggcctca
     2461 aaggtatcgg ctcccagatg cggggtgcag accacccgat caaaatcaaa caaggggttg
     2521 ccgatggccg gttctttttc ataaacgtcc aggccaacgc ttcttacttt gccggatttt
     2581 aagccttcca gcagcgctgc ttccttcatg atgccgccgc gggcacaatt caccagcatg
     2641 acgccctctt tggcatagga caggactttc tcgtcgatta tatgcgtcgt ttcttctgtc
     2701 ctgggtgtat gcacagtaat gatgtcggct tcgcgggcca gttcttccaa tgtctctttc
     2761 ttttcggcac catatttttc gaaacgctca tcggaaatat aaggatcata ggcgattact
     2821 ttcatgttaa aagctttcag ccgggtggcc accatcgagc cgattcggcc caggccaacg
     2881 atgcctacgg tcttgccgta cagttcggtg ccgcggaagg gctttctgtc ccaggtgccg
     2941 cctttgagta catcattggc ccaggcggtg ttgcgcaccg aggaaagcaa caggctgatg
     3001 gtctgttcag ccgcagaaat ggtattgctg tcgggggtat tggcaacgat gatgccataa
     3061 cgggtgcagg cttccacatc gatattgtct attccgttgc cggcccggcc cacaaccttc
     3121 aggcgagtgg cccgctggat caattcttca tttacctggg tgatgcttct tacaatcagg
     3181 gcatcatatt gatcaataat ggataacagc tcttcccggg aaataccgtc gcggaaatcc
     3241 acttccgctt tttctctgag aatagccact ccttcttcgg cgattctctc actgacaatg
     3301 attttcttct tttccacagt tattctcctt tcaaggttat gctattgctg atgcatatcg
     3361 aaaattgctt tttgggcaat tttaagcccg gttcccaatt ccagcgggaa tttaaagtga
     3421 agcagggtta tttccaaagc ggccagtacg gagagcagat ccaattccct gatatatccc
     3481 agatgtccca cccggaaaac cacattatca aggctttgct gtcctccggc cagaataatg
     3541 ttaaattgat caaacatata tttgcgaatc tgattagccc caatctccgg cggcgcaatt
     3601 acggatgtca ccgcggcaga ggcatagcga tcctcggtca gcagcttgag tcccaaggct
     3661 ctcaggctgg cccgcaccag atcacgatac gcggtatgcc gggcgatcac attgtccagt
     3721 ccttcttctg ccatcatctg cagggcctca tgcagtccga aatacaagga aatgggcggt
     3781 gtaaaaggcg tctgcccttt tagctggtat tccattccct ggtcaacatt ccagtaataa
     3841 ctggtattgg tattgctctt gtgcacttga taagctctgt cactgaaggc cataaagctc
     3901 aatcccggcg ggatcataaa ggctttttga gagccgctga taacgacgtc cagtttccac
     3961 tcatccatgc gtaattcaat cgccgccagt ccgctgaccg catcgaccat tagcaaggcg
     4021 ggatgatcgc ccatggcttc acgaacggct ttgacatcat tgtatgcacc ggtggaggtt
     4081 tcgttctggg tgatcagaac tgccttgatc tgatgatcag tatcctgcgc cagacgctcc
     4141 ttgatttttt gaggatccgc gccggttccc caggaaaaat ccattttctc caccacggcg
     4201 ccaaaattgc tggcgatcat ggcaaaacga tcgccgaaaa caccgatgga tacagccagc
     4261 acctggtctc cgggagaaat aaagttgact acggctgctt ccagaccgcc ggtaccggaa
     4321 gcgggataaa tcaacacgtt ggcacgggta cggtaaatct gttttatgcc ggccacgaca
     4381 tcttccagga tgtccttgaa ttcgtccccg cgatgattga tcatcggtcg gttcatggca
     4441 cgacagacgc gctccggcac cggggttgga ccaggcaaaa acaaatacgg ctcacctttc
     4501 atgctctaaa aaacctcctt atataaaaaa tcccctcatc ccggtcaggg acgagaggat
     4561 attcccgcgt tgccacccta gttggcgcat aactatgcgc ccgcttgaag gttataacgg
     4621 aaccaccgca actggtttgc agtctgcctc cagggtggat tcagaaacgg actatcctgg
     4681 tttgcagctc ccaccaggtc tcttgaatag tcccaaatca tactgatccc tttcatcgct
     4741 ttcctgtcta tggagtttgg gctccattat attatagtaa ctgagcattt tcaagttatt
     4801 ttgctacttt ttttagaaaa tactcgtgga ttccatcgtc tttggtcagc tccgggtgga
     4861 aagccgttac cagcagacga tcgtcttcgg ccataacgat tttatcgcta tggcgggcca
     4921 tgacccgggc ctctccccaa gctttttcca catagggggc acggataaat acacccctga
     4981 acggaccgat ccgatcaatc tccaactccg tctcaaaact gtccacctgc cggccaaatc
     5041 catttcggat tacagcgata tccattaatc ccaaatgggg ctgttccatg ccaatgattt
     5101 cccgggccag caggatcatg ccggcacagg tcccccagat ggccatgcct tgttccgcgc
     5161 ggttgcgaat cgcggtgtcc atttgatatg cctgcattaa cttgccgatg gtggtgctct
     5221 ccccaccggg aataatcagc ccatccacct gctccaattg tttgatgcta cgcacttgca
     5281 cgggttgggc gccgcagcgc tgcagggctt gctggtgttc gataaaagca ccctgtaagg
     5341 ccaggatgcc gattttttta ccagccacga tcctgcatcc tttcctccgg cttgatggta
     5401 tcaatattga tgcctaccat ggcttcgccc agatctctgg atacttcggc caggaccgcc
     5461 gcgtcattgt aataggtggt ggcggcgacg atggccttgg cccttttagc gggatcaccc
     5521 gacttgaaga tgccggagcc gacaaatatg ccatcgcagc cgagctgcat catcatggcc
     5581 gcatcagccg gggtggcgat acctccggca gcaaaattga ctaccggcag cgctttttgc
     5641 ctggccactt cctgcaccag ttcatatgga gcctgcagtt ctttggccgc agtcatcaat
     5701 tcttccgggg gcaaatggct gatccagcgg atttcatcgt tgacctggcg gatatgacga
     5761 acggcttcga taacgttgcc ggtgccgggt tcgcctttgg tgcgaatcat ggccgcgcct
     5821 tccccgatac ggcgaagggc ttcgcccaga ttgcgggcgc cgcatacaaa cgggactttg
     5881 aaatcatgct tgttgacatg atatttgtcg tcggccggag tcaatacttc actttcatcg
     5941 atataatcga cgcccaagga ttccagcact tgggcttcca cgaaatgacc aatccgacac
     6001 ttggccatga ccggaatagt aactgcttcc atgattttca taattatggt cggatcagcc
     6061 atccgggcta taccgccggt tgctctgata tcagcgggga ctctttccag ggccatgacc
     6121 gcacaggcac cggcagcctc tgcaattttg gcttgctcag gggtggtgac gtccatgatc
     6181 acgcctccct ttagcatctg agccagccct gattttaatg tgaatgttcc tttctcgacc
     6241 atctatgttc cctccaacaa atcgtattgg cttattcttc tttttcagac ttgaaacgtg
     6301 ccacggcagc gatgctgtca tcctgtgcga tcctcatcag cactactccg cgcgaatacc
     6361 gtttctggat ggatatatcc tcggtttcca aacgaatgat atgaccttcg ctggtcagga
     6421 tgatgatttc ttcatcttcc ttgaccactt tgaagcccac cacaggacca ttctttttcc
     6481 cgatctcgat ggctttgagg cctttgccgc cgcgatgctg aagattgaat tcctccagac
     6541 gggtcctctt gccgtagcca ttgagggtta ccaggacggc ttcgccgttg gtgcggtatt
     6601 tatcaacccc gacgacaaaa tcatttttcc ccaggttgat tccttttact cctgcagcct
     6661 gacggcccat agggcgtact tgtttttcat caaagctgat cgattgtcca taattggtga
     6721 ccagcatgac ctgatcatct ccgtctaccc gcaatacccc caccagttca tcgccttcgc
     6781 ccaggttgat ggcgatcaga ccggtttttc ggatattgct gaaggcgttc agggggactt
     6841 tcttaacgat cccacggcgg gtaaccatca ttaaatgacg gtgatcatcg aagatcttga
     6901 ccgccaccag agtactgacc ttttcatccg gtcgcaggtc catgatattg accagggcct
     6961 gtccttttgc ttggcgtgat gcctcctgaa tacggtaggc tttggtcgaa aaaaccctgc
     7021 cctgattcgt aaagaataga atattattta ataccccggt gaccagaatg tcaagcgcaa
     7081 aatcttcggc ccggatgttg gaactgatcc ccttgcctcc tcgcttctgc gctttatatg
     7141 tatccagcgg ctgacgcttg atatatcctc ggttgctcag ggtgataacc acttcatggt
     7201 cctcaatgaa atcttcctcg tcatattcaa cgttgtcgta ggtgatttcg gttcttcttt
     7261 tatctccgta tttggccttg atatcatcta gatcttcttt aatgattgcc agtacccggg
     7321 ctggattggc cagaatatct tcaaaatcgg caatgcgggc cagtaattcc tgatgttcat
     7381 cttccagctt ggctctctcc agaccggtca attgggcaaa gcgcatatcc agaatggcat
     7441 tggcctggat atcgctcagg gcaaagcttt ccattaattt gttgcgggcg gtttcgcggt
     7501 ctttggagga acggattagg ttcatgatct catcaatgtt gtcgatggcg atctgcaacc
     7561 cctcaacgat atgcaggcgg gcctgggcca gcttgagatc gtgaatggta cggcgggtaa
     7621 tcacgtcctg gcgatgttcg atgtagtatt tcaacatgtc tttcaaactt agcgtcttgg
     7681 gctcgccgtt gaccagcgcc aggttgttaa tgccgaagct gtcttccatc tgggtatgtt
     7741 tgaacagttt gttgatcagt acgttgacat tgacgtcacg tctgacttca atcacgatcc
     7801 gcatccctac gcgggctgat tcatcgcgca aatccacgat gccgtcaatc cttttgtctc
     7861 tgaccagttc ggcaattctt tccaccagtc tggccttgtt aacctgatac ggaatctcgg
     7921 tgatgataat ggcggttttg ccgccctttc tttcttcgat ttcatagcgg gccctgactt
     7981 tgatgcttcc ccgtccgctc tcgtaagctt gccgaatgcc ggcggcgctc atgatcccgg
     8041 aggtgggaaa atcgggaccg ggcaggactt tcatcagttc atccaaggta acatccggat
     8101 tttcgatata tagattcagc gcatccacga cttcggaaag attgtggggc ggcatgttgg
     8161 ttgccatgcc caccgcgatc ccggaagtgc cgttaatcag cagattggga atacgggccg
     8221 gtaaaacccc cggctccttg cgggtatcat catagttggg ccgccagtca accgtttctt
     8281 tgtccagatc tttgaccatt tcgttggcaa ttttggacat tctggactcg gtataacgca
     8341 tggctgcggc tgagtctcca tcgatggaac cgaagttgcc gtgaccgtca accagcggat
     8401 accgcatgga aaaatcctgg gccagtttga ccatggtttg ataaatggcc gcatcaccgt
     8461 gtggatggta tttacccatg acttctccga cgatggtggc ggatttttta tggggtttgt
     8521 catgggtcat gccttggtca taaagcgcat acagaatgcg gcggtggacc ggtttcaaac
     8581 cgtcccgcac atccggcagg gcgcgggaaa cgatgacgct catggaatat tccaggaacg
     8641 atttgcgcac ttctttctcg atgtcgatcg ggatgatttt attaaaaagg ttttcttgtc
     8701 ccaatttcta tatacccccc tggtttcaaa ccatatattt tttcttaaat atccaggttg
     8761 ctaacgtatt tggcattggt ctggataaat tccttgcgcg gttccacatt gtctcccatc
     8821 aggtcggaga aaacttcatc agcctgaatg gcgtcttcaa tggtaacctg caggatgacc
     8881 cggttgtctt catccatggt ggtatgccat aattgctccg catccatttc tcccaaacct
     8941 ttgtagcgtt ggagggccca ttttttgtcg gtctggctca tgaatttatt gagctcgttg
     9001 tcattgtaaa aatagtgaat ttcgttgccg cgcttgacgc catagagagg cggttgggca
     9061 atatagacat aaccgtgttc gatcaattca cgcatgaacc ggtagaaaaa ggtcagcaac
     9121 aagatccgga tatgtgatcc atccacgtcg gcatcggtca tgataaacaa tttgtgataa
     9181 cgggccttgc tgatatcaaa atcttctccg ataccggtgc cgatggcagt gatcagattg
     9241 cggatttctt cactggccag aatgcggtcc aggcgcgctt tttcaacatt gagaatcttg
     9301 ccccgcaagg gcaggatggc ctgggtgttg cggtcccttc cctgcttggc cgaacctccg
     9361 gcagaatctc cttcgacgat gaacagctct gaaaccgccg gatccttgct gctgcaatcg
     9421 gccagtttac cgggcaggga ggttgactcc agtgcgctct tgcggcgggt cagctcacgg
     9481 gcttttttgg cagcttcccg ggcgcggcgt gcggagatgg ctttttccag aattcgtttg
     9541 gcatccgccg gattttcatc aagaaaatca tctatttttt catacacggt gctctccaca
     9601 acactgcgta caaaggtgtt gcccaatttg gtcttggtct gtccttcaaa ttgcggttcc
     9661 ttgaccagga cggaaataat agcgacgatc ccttcccgga tgtcctctcc gttcaagttg
     9721 gcttcgcttt cttttaatat gccgttcttt ttggcgtaat cattgataac ccgggtcagg
     9781 gcgtttttaa acccaacctc atgggtgccg ccttcctggg tacggatgtt gttggcaaaa
     9841 gaatagatgt tttcggcata accgttgttg tattggatgg cgatttccac gacgacgtct
     9901 tcttttttca catcaaagta aatttcttta tcatgcaggg gttccttgtt tttgtttata
     9961 taggccacaa agtcatgcag tccatccgcc ttaaaggcct tttgatagcc ttccggatcc
    10021 cgctcgtcga tgacttcgat aagcagtcct ttattgagga aggaaagctc ccgtatccgt
    10081 tgtacgagga tctctctttc aaaaacgatt tcttcaaata cttcttcgtc cggctcaaag
    10141 taaatactgg tgccggtttc ggttgattcc ccgatgattt ccaacggtcc ggcgggtttg
    10201 ccgcgcatgt actgttgact gtaaattttt ccgtctcttt ttaccttgat caccatcaaa
    10261 gaggacaagg cattcactac tgacaggccc acgccgtgca gtcctccgga gatggtataa
    10321 ccgctgccgc cgaacttgcc gcccgagtgc aacgttgtta gaatgacttc gatggcggga
    10381 atgcccataa tcggatgggt atcaaccggt atgccgcgtc cattgtcttt gaccgttatg
    10441 ctgttgttgg gatgaataat gagttcgatt ttatcacaat agcctgcgac ggcttcatcg
    10501 atgctgttgt ccacgatttc aaatacaagg tgatgcagtc ctttcggacc ggtcgaaccg
    10561 atatacatac cgggtctttt gcggacggcc tccaaccctt ccagaacctg tatatctgat
    10621 gcattatagg cctgatctcc gtttatgttc ccgttgccgt ttgtcaaaac tttagacctc
    10681 ccttagaata tgttggcgcg ttttaaaagt gtttgggatg aaattggcga aaaataaact
    10741 cggtcgtcac atatcaccaa ggctttcttt ttttcctgag cggatacatt gaccagtttg
    10801 tgttccattt cggccagatc aagcacatcc tggatatcct ctgaccacgg atcctttata
    10861 ttaataattg caatcaggct ttgcgctggt attaagatgt catttcccag atgaataaac
    10921 atggttactc ctttccgagc aattcgccgt tggcaatggt gtatagtgat gctttaaaat
    10981 catcgggcgg attgaaattg actgagctta ggaacgattg gaattccgcc ttttgcaaat
    11041 actccagcaa acgatttctt ttttcttcat ccaactcggc cagcacttca tccaacagga
    11101 aaaccggaaa gtatcctttg attcgtttga atgtatacat ttccgccagt ttcaaacaga
    11161 ttacaatgtt gcgctgctgt ccttgggagg cataaatcct ggccggacgg ttgtcgaggt
    11221 agaaattgat atcatcgaca tgtggtccgg tcaagctctg ccgccgtttg ttttcttccg
    11281 gaaaaatatt ttttatatgt gccagtaaag cacctgataa aatattcaga ttaattttat
    11341 cactctctac cggaaatgaa agcgcatatc ttaacttcaa ctgattgccg gcatcattga
    11401 tttccttaaa caaaaaagag gcctcttcat ccagacggtt aataaaattg atccgttgca
    11461 agattacatt gacggcgctt tcaataaaca attgatcaat gatggcaaaa ctctgttgac
    11521 tagtttgctc tttcttgagc attaaattcc gcttttttag aattttaaca taattagcca
    11581 gcgcagttgt atattctgcc gataactgct gtagtaaaaa atcgatcaat gctcgccttt
    11641 ttgccggcga tccttttatt aaaaacagat catccggatt aaataatacc actttcaggc
    11701 gatgttggtt atgggtactg gtttttttat ggttgatggt gaatattttg ctgttgttta
    11761 tgtatgaaag agaactggtt aattcccgtt cctgataatt ataggccgcc aggatctgat
    11821 agcgttcggc atcataattt atcaactgtc ggtcattgct ggcacgaaaa gaactacctg
    11881 ctgaaaggac ataaatggct tccaacaggt tggttttacc ttgggcattg gtaccgcaaa
    11941 atatattgat ggcctggtcc ggagtaaaat ttatgttttt caggttcctg aaattattga
    12001 tgcttaattg actgatcctc aatgaaactc gccctgttct ctaattacta gtccgcattg
    12061 gcaccaggat atacaaataa ttatcctttt cattgttttt cagcatggcc ggtccaaacg
    12121 aaccggacag cgatattgat agattttcac attcctgatc aaaaattttg acagcatcca
    12181 aaaaataatg ggtgttaaaa acgattttaa tttccttgtc tccctctata ccgacaccct
    12241 ctataacttc attaatctcg cccatcatct cggaattgga gaaaatatgg acttcttcat
    12301 tctcaaagga cagctgtaga tgggggatct ttaatttgtc atcggtgggc atgattttga
    12361 ctctttccag actggtcgcc agaacatgtg tctgaatatc cagccgggta acaaaactgg
    12421 cgggtatgac ctgttcataa ttggggtatt gtccttcaat caagcgagac aggaaaagga
    12481 atccttcttt gtaaaaaata acgttgttgt cggacagagc gattttaatc tcttcatcgc
    12541 tgtcttccaa aaaacgcagc agttcattga cggtacgaat gggaatgaca aatttaaacg
    12601 gctcagccag ggtttcattt aaaacatggg tatagtacgc cagtcgatga gtgtcagaag
    12661 caaccacccg caactgattg ttgttttgaa ggtcaaacaa tacacctgta aacacctggc
    12721 gaaaatgatt ggcagcagcg gcaaaaactg tcttgcgcag ggcttctttg agaacatttt
    12781 gcgccatgga gaatttatat tccattttat cgatcggcag agcaggataa tcttgttcac
    12841 gatagatgtt tatattgcct ttggaacggc cataatgaat attaagtttg gatgtgctga
    12901 cgttcacttc catggcaata tttccatccg gcaacagttt gataaaatca gcaaaatagc
    12961 cggcattgac cagtacggta ccgggttcga tgacatctac attgctggta ctggcacaaa
    13021 taccgatttc catatcggtt gcggtcatgg tcagaccttt ttccggtgac agttccagca
    13081 agagaccgga tagtaccggg atggtatttt tatttgaggc tgcccgataa accatggtcg
    13141 tcaaagcaga caattctctt ttttcaatat tcatcttcat tttatgacct ccgcccttat
    13201 tatttattat ttattaaaaa ttagtcgtaa ccgtagtagt agtgtgtaca ttgtggacaa
    13261 gctccgggat gcaggatatc atgcttttta gaagtatcga acaagtttgt tgataacttg
    13321 tccccctact cggcaaaatc cggatcaatt tttttacaga tatctttaac cgaggtataa
    13381 agttcggctt gttcttccat ttcccgggtt attttttcca cagagtgaat gacggtggta
    13441 tgatctcttc cgccaaattg atcaccaatc aaaggcagcg acgcgtcagt catcctgcgg
    13501 catataaaca tggcaatctg acgtggcaag accaggtttt tatttctttt cttggataac
    13561 atttcgctta gttcgagtcc ataaaattta cagacttcct tctggatgca ttcgatggtt
    13621 atttttttcg ggccgggtgg gggcaaaata tcttttaacg caatggtggc ggtattcata
    13681 tccaaaggta atttgttgat ggtagcaaat gccaccagtt taaccaatgc cccttccagt
    13741 tcgcgaatat tggaatcaat gtaattggca atataatcga ttacatcata aggaatattg
    13801 aaattgtcat tttgtgcttt tttcctgaga atcgccaccc gtgtttccag atcaggggtt
    13861 tggatgtcgg tgatgagtcc ccattcaaaa cgtgatcgca gcctgtcctc caacgtggga
    13921 atattgcgcg gtgggcgatc actggagata accaattgct tgttggattc atatagggta
    13981 ttaaaagtat ggaaaaattc ctcctgggta cgctccttgc cggccaggaa ttggatatca
    14041 tcgattaaaa gaacatcaat attgcggtat ttgttgcgaa aacctgaggt attgtcatct
    14101 ttaatggaac cgattaattc attggtgaat tgctcggaag aaacatacat gacagagtat
    14161 ttgggataat ttttcacaat gcggtggccg atggcctgca tgagatgggt tttacccaga
    14221 ccgaccccgc cgtaaataaa cagtggattg tatgcccggg aaggactttc acccactgca
    14281 tagcaggcag catgggcaaa acggttacta ttgccgacca caaaggtatc aaacgtatat
    14341 ttgggattga aaggtgatat ataggtattc tttttaatag actcggtggt cagaatcaga
    14401 gatatatcag gactgctcaa agcacggaaa tacttttgca tgttttccag ataacgcgat
    14461 tcaatccatt ccttggtgag ggtgttggga acggtaatgt aaattttctc atcacggctg
    14521 gtgttgtact tgactttgga aaaccagata tcaaaactgg tagaaccgat ctcttgccgt
    14581 actgcgtcta ataccttctc ccaaactgcg accaggtctt ggttagtgga cataaaaaaa
    14641 ggcctcctgt tgatttgtgg aaaaaccccc gggggaaaac gataactttg tggatattat
    14701 tagaatcata tagataaata tatatataaa ggaaagcaag gttttaaatt gcttgtaaaa
    14761 aacgcccata agtatagtat atttattaat catagcagat tcaaacaaca aatagaagtt
    14821 tttagaaagg ccctcagcct tgacattaac acgcttttgt aaatataatg attaagtact
    14881 ggaaaaatta agggaggcac accaatcgtg aaaatgacat atcaacccaa aaagagacag
    14941 agaaagaaag tacacggctt cagaaagaga atggcaaccg ccagcggcag aaaagtgctg
    15001 gcaacgagaa ggaaaaaagg aagaaaggta ctttctgcat aggtcgtttg caaagcggcc
    15061 ttttattttc tttacaggca gctctcagcc aggcaaagcc cggtgcgcag gaaaatgatt
    15121 gtcgggcact ttgaaaagag agaagatttg cggccgcggc gttcgctttt ataagcgcag
    15181 gccgggcttg acagtacctt taaaggaaac agaatgcttc aaaaaaagta caggatcagc
    15241 aacagggcag actacaactt tatataccaa aacggtaaga aaatacattg ccgatatatc
    15301 attgttttta tcactgccaa tcaattggca tataaccgtt ttggcatcgt aacctctaaa
    15361 aaagtcggca atgctgtgca cagaaatcaa gccaggagaa ggctgagagc aatcatacaa
    15421 aagcacatgc cggaaataaa gcaaggttat gatctggtaa tggtagccag attcaatatt
    15481 aaggaagcaa tgtttgcagc attggaaaaa gattttctga tagctgccaa gaaggccgga
    15541 ttgatttgag taaagtgttt atatttttca taaagcttta tcaaaagggt tcctttttaa
    15601 agcggccttc ctgtcgtttc taccctacct gttcccagta tgcggttgag gcgatcacca
    15661 agtatggtgc agcaaaagga ggatggttgg cactcagacg aatcagtaga tgtcatccgt
    15721 ttcatcccgg aggttatgat ccggtgccat aataaacggc aaaagtttta ttgtttgaag
    15781 attaaggagg tggcacctgc tatagaaacg agaaatcgat agcagggttt taatttgtgg
    15841 agcagttttg ttcaatggtt tgcgggagtc attgatttca cttatagcct gactgtgcaa
    15901 atgggacttc ccagttacgg tctggcaatc attctgatga ccatagccat aaaaatattg
    15961 atgtttccat taacgcagaa acaaatgcat tcaatggcgg ccatgcagga gattcagccc
    16021 aaaaccaagt atattcagga aaaatacaaa gatgatccgc aggtcatgca acagaaaatc
    16081 atggaattat ataaagagca tggggtaagc ccttttggcg gctgtatgcc cctgctgatt
    16141 cagatgccta tttttattgc cttttaccaa gccctcttta atttcaaatt tgtcaatctg
    16201 gcgcatgccg gtttcttatg gattcccaat atcggtaaac cggatcctta ttttcttctg
    16261 gccattttgg cggcagccac cacttactat caacaaaaaa tttccatggt ggacagtaaa
    16321 gacccgaccc aaaagaccat gctctacttt atgccaatat tcatggcctt tattgcctac
    16381 aaactgccgg ctggattgcc gttgtactgg gtcgtattca acatcctggg aattttacag
    16441 cagctctatg taaattccca aaaaagaaaa gtggcagcaa gcacaggaac agcatccctt
    16501 gtcatggaaa cacaggataa tgtcaaacct tccgcagcag caaaggctga cagcaagggc
    16561 gattctgccc aaaagcagca agcagcaaag gcctcggaag gtggcaatac caaggacgct
    16621 gcgaaggaca aaggaggaaa ggcaaagaat gcaagtccaa acagtagaaa aaaagggaaa
    16681 aaccattgat gatgccattc aagccgccct ggaagagctg ggttgcgggt tggatgaagt
    16741 ggaagtcgag attctggaag aaggaagtaa aggactgctg gggttgatcg gcagtaaacc
    16801 ggctgccgta aaagtatccc gcaaagttga caatccggct gaagatgcac gccaattggt
    16861 ggaagagctg ttgaccagaa tgaaaatcga ccacgagatt gaagccgtta ctgccgaaca
    16921 aggtacggta agaatcaaca ttattggcaa agacatggga ttgttgatcg gacgcaaagg
    16981 agaaacacta aattctttac agtacatcgc cggattaatg atcaatcgca aacggggaga
    17041 aaggatcagg ctgatcctgg atgttgagga ctatcgcaaa aaaagagatg agtcattgga
    17101 atctctggcg gtcaggcttt ctgaaaaggt taaggcgacc cagaaaaatg ttgttatgcg
    17161 gccgatgagt tcccaggaac gcagaatcgt acatacggca ttgcaagaag atccgcaggt
    17221 agtgacgtat tcgatgggcg atgagcccaa tcgcaaggtg gttattgctt taaaaaaata
    17281 acaaagaagt gaatgcagca agaccatgct caccagcgtc cgccgctcag gcggtacgca
    17341 ggtgagtttt ctttattcct ttgtggtatg atattttaaa aacaagcaag tatttgatgg
    17401 agtaaaaacg atgaacaacg acattattgc tgcggtttcc actccgcctg gcgaaggtgc
    17461 ggtagggata attcgcttga gcgggcacgg ggccatagaa atcgcggaga aaacatttct
    17521 tccttataat aaaaacgttt ctctgcgcga acgccgcaat ttttccctta ctctcggctg
    17581 gattctggac gcagagggaa ttagaacaga tgaggtgctg gttggcatta tgcgcgcgcc
    17641 gcacagttat acgggagaag atgtggtgga aatcaactgc catggaggcg gggccgcgct
    17701 gcgtgcctgt ctgcaacgat gtctggagac aggggccagg ctggccgagc ccggggagtt
    17761 taccaaacgg gcttttttaa atgaacgctt ggggttggag caagctgaag cggtactgga
    17821 gattatccgt gccaagacgg aacgcggatt gcaactcgcg gttcggcaac tgtccggcta
    17881 taacggccga tttatggaca atctggagga gcggttgctg cggttgaatg ccatggtaga
    17941 tgccagtctc gattttcctg aggaagtggg cgatctggat caggacgaag cccggcagct
    18001 cctggatgga atcatagcca ctattgatga ctggatcaat tctgcacgtc gggccgagat
    18061 ctatcgggat gggattgaac tggccattgt tggcaaaccc aatgtgggca aatccagcct
    18121 tttaaatgca ctgtcccgca aggaaaaggc aattgttacg gatattcccg gtacaacccg
    18181 ggatgtggtg gaagaataca tcaatgtccg ggggattccg gtaaaaataa tggatactgc
    18241 cggcatcaga gcgacagagg atcgggtgga aagaatcggc gtggagaaat cccgggagtt
    18301 aattgaccag gccgatttgg tggtgttggt attggatttt gcagccggta ttgccgccga
    18361 agacctggaa atatttcaaa caattccccg ggaaaaatgc ttggtgctgg tcaacaagga
    18421 agatattgaa cgcaaagcga ttacagaaga ggagctggcg gaaaaatttg cttctgcccg
    18481 gctcatccgg gcttccgtga aggaggatta tgggctggat gaattggaac gcgctatcga
    18541 ggaacttctc ttgtcgggga cccagaatgc agacggaatg gaaattatga ttaatttgcg
    18601 gcagaagaat gcgctcttga aaagccgcgc ttacgctgaa aacgcccgcg aacaattggg
    18661 aaggatttcg cttgattgtc tgggtgtgga tgtctggggc gcgctggaga gtttagggga
    18721 gatctcaggt aaatcgctga aagaagaagt ccttgaccgt atctttcatg atttttgtat
    18781 tggcaaatag cagcattagt gggggttata aatgatatac gaggctggaa aatatgatgt
    18841 ggtagtaatt ggtgcgggtc atgccggctg tgaagcggct ctggcagcag cgcgcatggg
    18901 ggtcagtaca ctgctggtta ccctcaatct cgatgctatt gcgctcatgc cctgcaatcc
    18961 ttctcttggc ggaccggcca aggcccagtt ggtgcgggaa attgacgcgc taggcggaga
    19021 aatggcggta aatattgata aaaccaatat tcaaatccgg atgattaata ccggcaaagg
    19081 accggcagtt cagtccttgc gggcccaggc tgacaaacag gaatatcaga aggaaatgcg
    19141 caggacgatc tttcaacagg ccggactgga tctgctgatg gcggaaatca tcgcaatcga
    19201 aactgttgat ggacgggtct gcgctgtagt tacccgtacc ggcgcccgat tccggtgcga
    19261 tgctgtggta gttacctccg gaacatatct caaaggccgg atcattattg gcgatctcat
    19321 gtacgacggc ggcccaggca atcaataccc ggcagcggaa ttatcaaaga gcttgttatc
    19381 tctgggcatt cagctgggac gtttcaagac cgggactccc ccgcgggttg atcgcaagag
    19441 cgttgacttc agcaagacga tcgaacaacc gggtgatccc gatgtacctt ctttttcctt
    19501 tatcagcgac ctagcaccgc gcgagcagct gtcctgctgg ctgacgcatt caacgaccga
    19561 aacccatcgg atcgttatgg acaatatcga aagagcgccc ttgtttaccg gcgttatcaa
    19621 aggaaaagga ccgcgctact gcccttcctt tgaagacaaa gtggtgcgct ttgcccacaa
    19681 ggaatcccac cagttgttta tcgaaccgga gggtcgcgca acggatgaaa tgtatgtcca
    19741 gggactcaat accagcctgc ccgaggacgt acagctggaa gtgctgcgca gcattcccgg
    19801 catggaaaac gtgcgaattt tacgcaccgg ttatgccatc gaatatgatt atgtgattcc
    19861 ctcccagcta aaactcagtt tggaaagcaa agtcgtggaa gcactgttta ccgccggaca
    19921 gatcaatggt acgtccggtt atgaagaagc tgcagcccaa ggtctgatcg ccggcatcaa
    19981 tgctgcctgc aaagtgcaaa acaaaccgcc ctttgtactg aaacgcagcg aagcctatat
    20041 cggggtgctg atcgacgatc tggtcaccaa ggaagcagat gaaccgtatc gactgttgac
    20101 ttcccgggct gaataccggc tcttgctgcg ccaggacaat gctgatttgc ggctcacaga
    20161 aaaaggacgg gaaatcgggc tggtcagtga ccagcgctgg gccaagttta tggataaaaa
    20221 aagacggctg gaagaaatgc gcagtctgtt ggaaaaaacc acctttacgc cggctgaccg
    20281 catcattacc gcctatctcg aagagatcgg cagcgctatg ctcagagaca gggtctcctt
    20341 atgggaactg ctgcgccggc cggaagtcac gatagacgat ttactaactc gcagtctggt
    20401 tccggaaatg aaaaaggaaa tcagtgaaca attggagatt caatccaaat atgaaggcta
    20461 tattcaaaaa caacaggagc aggtcaagcg ttttgaaaag ctggagagca aagccattcc
    20521 ggcggacatg gaatatcaga acatcaagac cttgtcctcg gaagcgatgc aaaagctgga
    20581 acagtttaaa ccggaaacca tcgggcaggc ctcacgcatt tcaggcgtta ctccggcgga
    20641 tgtaaatatt ttattaattt ttttaaaaaa gagagaagga attaagtaat ttttatggaa
    20701 atatatgtaa accgctttat tgactttttg ttcgccgaga atgaaaaaca aaacctgatc
    20761 agcagacagg cagggcggga agatgtattg aaccatattg aggacagtct ggccattttg
    20821 caatttatct cgctggccgg aaagcgtgtc attgatatcg gcagcggtgc aggatttccg
    20881 gctatcattc tggccatgca ttgtgaggat gcacagtttg tcctggtgga atctgatctc
    20941 aagaaaagca gtttcctgga gtcatgccgg ctgcagctca acctgaaaaa tgtccaggtg
    21001 gtacgcgcca gagctgaaga aatcggtcat gacaagcagt accgggcgca gtttgattgt
    21061 tgcagtagcc gggcggttgc ggccatgaac atcctgctgg aatatggcct gcctttgctg
    21121 aaaaccgggg gcaagatgct gttatggaaa ggcctcaatt atcaggcaga actggaaaca
    21181 gcacaacatg cgttgcaagt cttgcgggcc ggggtggaag ggatttgtcc ttataccctg
    21241 ccgggagacc ggcagcggat tattgtggtg gtcaacaagc aagcggaaac gtccccgcag
    21301 tacccgcgtc gggtggggat tcccgccaaa aggccgctgt agtccgaagc gtttccagaa
    21361 taaatagtcg ggcggttgtg gggaaggagg gaatcacata atggaggtca aaaaacaact
    21421 tgagaagata cttcatgtgg gaagcggcag ccgggttttg gaactgccgc tggaacaaat
    21481 cagcccgaat ccatttcaac cgcgcaaaga gtttgatgaa ctggaactca aggaactggc
    21541 taaatcgatc agcagttttg gcgtcattca acccatcatc gtccgcaaaa gcaaacaggg
    21601 ttttcagctg gtggccgggg aacgccgcta tcgcgcctgc aagctgatcg gacgacagca
    21661 aattccggca attgttcagg acatcgatga tcaatccatg gcggccattt ctctgatcga
    21721 aaatctgcaa cgcaaggaac tgaattattt tgaagaagcc atcgcctata gcaccttgct
    21781 gaatcgcttt ggtctaaccc aggaagaact ggctcagcga gtcggcaaaa gccagtccgc
    21841 gatcgccaac aaattaagac tgctcaaaat cgcaccggaa atcagaaacc tcattatcac
    21901 caccaccatc accgaacgcc atgcccgcgc cttgcttaaa ctcaatacta tcgaaatgca
    21961 aaaggaaatc atccgggcca tttacgaacg cgaacttacc gtcaaggaga ctgaagagct
    22021 ggttgaaaag gcgcagcgca acaatattcc ccgtgaaccc aaaacctcga acaacggaca
    22081 gcagatctcc atggttatca gggatgcacg cattttttta aataccatta aagaaaccgt
    22141 caaccgggcc aaacagaccg gaattgacat gcgggtgttc gaaaacgaga acgaaaatga
    22201 atatgagatc accatcaaag tcaagaaact acgcaagaat agccgcctga tggcagaaat
    22261 ataaaagcag aaatgtttca cgtgaaacat ttctgcccgg ttgaaaaaat tcatgtttat
    22321 caggaagcca gttttgttgc cgattttcac ggccgcattg cgcaaagcca caatcggtct
    22381 tgataataaa aaatttaaaa tgtttcacgt gaaacatttt ttatatgata aaaaaatggc
    22441 tatgcattga atgataccgg aatacaggca ttgatgtcta tgttccggct ttttttcgga
    22501 aaatgaaaat atcgtttgga acaaaaatta tgcagaagta agttgaaaat gtagtagaat
    22561 aataaaaggc aaaagcaaaa gccagagagg gggacggcgg atggcaaaga tcattgcggt
    22621 ggcgaatcag aagggaggcg taggcaaaac cacaacttcg gtcaatttgt ctgcgtatct
    22681 ggcgctggaa ggctatcggg ttttgttggt cgattgtgat ccgcagggaa acgccagcag
    22741 cgggttgggc ataaaccgca aaaaaattcc gtattgcgtt tataatttgc tgatcgacgg
    22801 gatggaaata aaaaaagtgc tctgccagac ccgcatcaac aatttggtga ttctgccggc
    22861 aacgattcaa ttggcagggg ctgaagtgga actggccaat cgcccgggcc gggaattcat
    22921 tttgtcgcag gcgctgcagc cggcgcgtga tgaatatgat tacattattc tggattgccc
    22981 gccttctctg ggcctattga ctattaatgc gctgacggcg ggaacgtcgg ttttaattcc
    23041 cttgcagtgc gagtattatg cgctggaagg attgagtcaa ctgatggata ccatccagct
    23101 ggtgaaaagt cgcctgaatc cgagtttgcg ggtggaaggg atattgttta ccatgtttga
    23161 cggacggacc aatctggcca ttcaagtggt ggacgaagtc aagcgccatt ttcataagga
    23221 agtatatcaa acggttatcc cccgcaacgt caggctgagt gaagcgccca gccatggcaa
    23281 acctatcgcc ttgtatgatc cgcgctctaa aggtgcggaa gtatatagag atttagccag
    23341 ggaggtcatt gaacgtggca aaaccagaaa gaggactagg tagaggactg gatgcgcttt
    23401 tttcggtgga agaaagcatc gatgaaaagc tggttgccga aatcgagatg aaactaatcc
    23461 ggccgcgcaa aaatcagccc cgtaaaaact ttgatccgga acggttgcag gaattggcgg
    23521 agtccatcaa ggaaagaggc gtgctgcagc ccatactgct gcgtccccgg gaaaaaggat
    23581 atgagattgt tgcgggagaa cggcgctggc gtgcctccaa aatggccggt ctaaaaatga
    23641 tccccgctct ggtaagagaa atgagcgatg cggaagtagc agaaatttcc ctgattgaga
    23701 atttgcagcg ggatgatctt tccattattg aagaagccct ggcatataaa aacatgatcg
    23761 aagaatttga ctaccgccag gatgttcttt cgcaaaaaat tggcaaaagc cgggtgcata
    23821 tcagcaatac catgcgactg ctcagcttgc cccaggaagt gatcagttta ttggaaaaca
    23881 agcaattgac ccccggacac gcccgtgcct tgctgcggct ggacagcgac agcgcccaga
    23941 ttaaagccgc ccgcaggatt gcagagggaa aattgtccgt acgtaaggcc gaacaaatca
    24001 ttcgctttga ccaaaaggag gccaggcccg cgcaaaacaa tactgcagag gagatggatt
    24061 tgcaagagcg actgcaaaaa catctgggca gcaaggtgga aatcaaacgg cgtcgccagg
    24121 gcggcagcat tgaaattacg tactatgatc atgaggatct ggaaagaata cttgaaatca
    24181 tgggcctcaa gtaagctggg gcagcaattt aaaaaacaaa aatgtttcac gtgaaacatt
    24241 tttatagtgg caagtgaaat aaattgccac tttttttatt gcataaaaag agagaaaacc
    24301 gtactgtaga atcgctggca gatccggctg gaggttccgg taatagcggc ctatcaacat
    24361 ttattggcgg gaatcggatt tttggctatt gccttgctga ccggacaagc ttggctgcaa
    24421 gcacctcatc ggcctggctg gcgctggctt atttgattat ttttggatcg gtgattgcgt
    24481 ttacctcctt tatcagtgcc ttgcagttac tgccgatcaa tatcgctatg acctatgcct
    24541 ttgtaaatcc tgtattggct cctgtattgg cgcaaatatt aggcgggtgg gtcctgggcg
    24601 aaccacttac gctgctcgga acgacaatgg ttatcctggg agtggtcagc gtatttgcca
    24661 acaggaatca agccgacata aaagaaaaat cccctttcca tcgggctgcc tagacaagaa
    24721 ggcgtttgat taaaaccgta aaatgtttca cgtgaaacat tttacggttt ttgttggcaa
    24781 gcaagcccca aaagtatgga catttttact tccgcttgag cgttcgtttt gatacagaaa
    24841 aacaactcca gcgctgccct cagattcaat tctgcgaaac gcgatacatg ggatggatag
    24901 tggttgattt tgcacccaaa gcagattagg acaacgttat atatataaac gccgtatctg
    24961 aactggatac ggcgtttttt atggtgaatg atttctgcca aaacaccggc atatcgcgaa
    25021 cggatgggat tgcaaataca tatagtagaa tagcccgtcg aggagatgtt taattttgtt
    25081 aatagtagtg aaaggaggga aatgatgaag aaaaaaccgc ccagcaaaat aaggaaaacc
    25141 ggaataaaaa cgataaaagg aaagaaagca ccgatctata taaatgtaaa tatgaaaaac
    25201 agctcactcg gcacaatcac aaattgtatt cagccgacga accatccctt gttggaccgg
    25261 gaaattttaa aaccgtcagc ctgccagaca tggaaaacag tctgctctgc tcccggctat
    25321 aattgcgttc cccgccaagg tgaacgggtt atcaatggtg gctttgaaaa ccatccggat
    25381 ccgtttttgg ggtgggtcat caatgccgga gtcgggttaa tcaaaccatg gcagggagat
    25441 ctcccccatc agggacgaaa cgctgtctgt ttaggcaacg agcatggctg tgcgctcttg
    25501 tatcaggaca tcagcgggat ttgtccgggg atgtatttgc aattgagtta ttttctaagt
    25561 gctggcagcg catgcggcaa tgacgaggtt ttcatcatag tggagtttct ggatcgctgt
    25621 aaaaagtcga tcgatgggcc ggtgctggag atcctggtac cttgcaacag tctcagcaat
    25681 gaaacctaca ccggttttct gaatgccacc cggagagggg caccgcctgg gaccaaattt
    25741 gtccgcattc gttttgcggt tgatggtaac tgcgcaggtc aaagcaggcc tattcacctc
    25801 gatgatgtat cgctcatcgc catatgagtt caataaagag gggcaggctc aagaacgatt
    25861 gtcaccaaac gcaaaggagg ggcatacagt atatcagtaa tggcaaatca tagttttata
    25921 cccagtaaat gccatgcaag aaagctgtca aatcaattca tcattgagga ggcgtatttt
    25981 gtgaacaaag gcaaagaaaa gtgttgttat gatgaagaag ttatctacga gccggaagaa
    26041 atggaatgca aaccgcatat tccacggcac ggcgaaacag aagtcatttt gcattgtggg
    26101 accgttactg gttcggggcc tttatcagac tacgttaatg gtggttgcgg accggctgca
    26161 accgtccagg caagtgtggt tcttgatacc gaaaggctga gaaaacctac cgttaaaatt
    26221 gatttttccg ctttgatttc gtataagacc acctatgagg attatttcct gcatctgggc
    26281 tttagattga gtaaaatttg cggaggggca actatcccgc tgggcacctg ggtctttgaa
    26341 aaaatccacg gtccgaattt taacccggga aactcagcag cggaaagacc gccattacca
    26401 ccgaactgtg acgtgtttca ggaaacggat cccttctttt ttagctggtg tgcttgcggg
    26461 gactgtccgg attgctgtcg ttatatcgta gaactgatcg accaacagta ctacaacgtt
    26521 gaatacgcag tagtcacgaa tattgcgctt accgccatgg ccgtcggaga aaaaagaaat
    26581 gtttaaacca tagccaggtc agacaataaa agacctgcct taatgagggg agcatatcac
    26641 accgcaaggc aggtctttta cacaaattgg aatcattaca aacaggctgc tgacgtctct
    26701 gtagacgcat tcagcctgag aagacgaatc agaacattaa aaaagaaaaa caggggcctt
    26761 aaaagggcag ctggaatccg aatccggcga atggtaaaaa ttacctaacg ctttgtggaa
    26821 tagaaagcag agatactgcg atagctccaa gcaattaatc agggatatcg tttaaccggt
    26881 tttgcacaac ggcaaaaacc tctcgcaggg agagttggtt ttgacgggcg atggcctgac
    26941 agtcttcaaa ttcagctttt ttataaacgg ttccgtcgtc tgcccgggca actttgatgc
    27001 gtacctggcc ccaaggggtg gacagcagct cctgatgacg cggccaggca aatctctcct
    27061 gaaagcggta acgcacaccc agggtgccgg tttcccgcat taacagccgg caaatctcat
    27121 cggctgcagt acgggcagtc agaacggtaa tcagcgtgcc gggccggttc tttttcatat
    27181 agaccggggt ggtaaaaaaa tccatcacgc cgggatgatc cagacagaga cggtagaggt
    27241 gggtaaatat ctcgggatta agatcgtcaa cttccgtctc cagcacggcg atggtttccc
    27301 gctgaccaag gcgttcatcc gcttgggcac ggatcatccg cagaagattg gggaccttgt
    27361 cttcacgctc ccggctacca gctccataac cgaccgccag tggaacaaaa gcgtttggtg
    27421 ccgcctggtt tttaaccaaa tgggtcagca aggcagcgcc ggtgggcgtg accagctcga
    27481 taccggcagc tgaagagaca caaggatagc cttgcagcaa ccgtacgacg gccggtgcag
    27541 gcagaggata gcgtccgtgg ctcatgtcca gcacgccgtt tgcccacggc agggcagaag
    27601 catagacctg ttcagcgccg aggtcggcca ggcacaaaaa ggtgccgacc acatcaacga
    27661 tggtatcaac cgccccgatt tcatgaaaat ggacccggga catgtcaatg ccgtgtactt
    27721 cagcttcagc ttgggccagt cggcgaaaga cagccaggga atcctttttg atcgcttccg
    27781 gcaaggaact ggtctgcagg atggcttcga tttgctccaa atggcgcagg ggaggggcgt
    27841 caggacaatc tacccggaaa gtggtacaac tgatcccttg ccggacttgc ttggctgcgg
    27901 cgagccgggc ccctaaattt aaagcctcaa taccttcctg caaagctgtc agagaggcac
    27961 caccatccag cagtgctcca atcagcatat caccggaaat tcctgaaaaa caatccagat
    28021 aaacaaaatg catcttcaac ctcccctgcc cccgattatg ttaggcggca gcttatgggg
    28081 ctgccgtttc gtttcttttc ctatttatta tactcattaa ggcagcatat aggccagcag
    28141 actttacatg cagcacaacc gggtcagaaa gtggggaagc atcctgttcc ggcagcgagg
    28201 agggcaggga gggattaaag gttggcttga ttggcataag gcagcctctg atttttctca
    28261 ttttcaaaat gcaggtgagc gaccaaaaga ctgcgggcga tcacctcagc catgcgatag
    28321 acgagaaaga gacgcgtgtt ttgcaagacc atctgctcca gataaccgcc cacatttacc
    28381 accccggaga tatgaaaatc ccctacttcc ggcagatcct tgtgcaaggc agtaccgggt
    28441 ttgagaccgc cttttttgac attgataaag ccaatccggc tggaattgcc caggctggca
    28501 tcaatggcta ccgtcagagg attgtcatag cgacgagtga tttgctcggt aaactgggtt
    28561 agattggttg cgtggacagg ctgttccagt gtcccgtaca catacgggtg cagggaagtg
    28621 gattgcagcc gggtaccaac cataggaccc agacagtcac cggttgcacg gtcggtaccg
    28681 atacagatat atatgatgtc tttttcaaaa ccgggattca tattaattag atgggtgtaa
    28741 acaatttttt gcaaatgtcc gaaacaggcc ggatcctcaa aatggaagga atgcggatca
    28801 gacgaatcag atgtgaaaat gctcatggca acccccaatt aaatcattta taaatacttt
    28861 tgccattttg tcagtatttt atgctgtgca gctgatggag agagaaaaaa agggcaggtg
    28921 acaggcataa aaaaacagct ggtaatcacc atgggatatc tgggggcagt cattggagca
    28981 ggttttgctt ccggtcagga aattgtacag ttcttcgtct gctatggtaa gtggggagcg
    29041 gcgggggcag tgctggcgac ggttttgttt gccgtttgcg gcgggatgct gctcagcatg
    29101 gcacaccggc aaaagattgc caactatcaa gctatgttga gtataatgtt cggtccccgg
    29161 ctcggaaaaa tcgtcgattt cctgctggcc atttttttgt ttttaggctt gagcaccatg
    29221 ctttccgcca gcggagcggt gttctatgaa catttgtatc tgcccaagta tgccggcata
    29281 ctgctggcgt atgtagccgt tgtcatcgcg ctggcggcgg gaaaaaagag cctggtcttt
    29341 tcctataacg tgctggtacc ggtaaaaatc attctattat tgatgatttc cggctatatt
    29401 gctctatttg ccggagaagg acagagcagt gtttatacca gctatctcat tccccgcggg
    29461 gaaaacgcct ggctgttgtc gagcctgctc tatgtcgcgt ataatttttc cctggccatg
    29521 gtggtgctga ccgcatatca aagcgtcagc aaccgccggg agggaattgg cggagcggtt
    29581 ctgggcggac tggtgctggg cggactggtg ctgctcaact atgcggcgct gtccaaatat
    29641 ttgccggcgg tgattcacta tcaggtgccg atgctctata ttgctggaca ggtgtcgcca
    29701 ttggccaagt ctgtctatac cctggtttta tggatcggca tcctgactac agccattgcc
    29761 aatgcctacg gttttgcaca gcgttttgcc cgattcaccg gcattgatta tgcggtctgc
    29821 ctgatcgttt gtatgacact ggccctgccc attgccaccc agagtttttc cagtctggtc
    29881 aaccgggtct atccgctttt tggcttgctg ggcctcttga ttctgtccgg tctggtctat
    29941 cattttctgc gcagccgggc aggtaattaa gaaaagcaat gctgctgcaa gaaaaaagct
    30001 cccggcggga aggttatagt cagggaatcc ccgctgagaa tatacaattg atcattcccg
    30061 tgctaaactt tagagaacca tagaattttc atgggaggaa agctctgatg accaatctga
    30121 gaaacattcc atcgatagac gagctgctga cccagccgga aacccggcat ctgctggctg
    30181 tttaccagcg tgaattcatt gtggatacgc tgcgtcaggc ggtcaacgaa gtgcgcacgg
    30241 agttgcggac atctcggcaa acgtttgcca aagcagaaat cagccgattg gttttacaaa
    30301 aggcggaagt gagtttaaaa aatactgtcc gcggcagcct gaaccgggtc atcaacgcca
    30361 cgggcgtcgt tttgcatacc aatttgggac gagcgccgct ggcggatcgg gcatttcaat
    30421 ata
//
LOCUS       DAOTHZ010000020        30212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1709_length_30212_cov_6.805253, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000020 DAOTHZ010000000
VERSION     DAOTHZ010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 30212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30212
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_30212_cov_6.805253"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="larA"
                     /locus_tag="P5549_04000"
     CDS             complement(<1..875)
                     /gene="larA"
                     /locus_tag="P5549_04000"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HRW12264.1"
                     /translation="MIKLAYGRSHIELQLNEKNLAAILQPRHLQSAGGGMEMVEESLA
                     HPVASPALREIIAAKEARSAVIVVNDITRPTPYELILPPLLREIEQAGIPDQKVTLVI
                     ATGIHRVHTQEDNLAIFGAGICRKYRIINHDCDNDLKFLGYLSNGMELIINRTVAEAD
                     LLITTGVVGLHYFAGYSGGRKSILPGVAARAAIEANHKMMSDPRACLGNYENNPVSDL
                     MLEAARKAGVDFIINVVLHSHHNIAFCASGDVEAAWLAAVQYAEQMSVLRIDAPADIV
                     IASCGGYPKDINMYQA"
     gene            1228..2628
                     /locus_tag="P5549_04005"
     CDS             1228..2628
                     /locus_tag="P5549_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRW12265.1"
                     /translation="MDSAAIRKELVKLLGKDKVLTEPLDLMYYSYDSSFLTQMHPADP
                     YAVVFPKSTAEVQQVMRFAYENNINVIPRGAGTGETGGCIATEGGIVMELSNWDEIVE
                     IDAKNMQVIVRPGVIHAKLNEELEKVNLFFPPDPGSSQMCTVGGMVANNASGLRAVKY
                     GTTEQYILGLEVVTPTGDIIYTGGMKARCIKNVSGLNLTKLMIGSEGVLGIVTQIRLR
                     VWPKPKARGIAMAVFDQLDDAPATVLEIYQGGLLPSGIEILDSSAIKAVNEFKPEINL
                     PDCEAILLFEVDGNPPSVEYEGEQIKEIAGRRARLVEWSTDTKRMAQLWEGRSVTASA
                     ASRVRADGTRVFAGEDISVPLAKVADTLRAIRQLAAKHGIQVVNYGHIGDGNVHTAPV
                     INPEIPDEVERVMQLVDEIHLLAIEMNGSTTGEHGVGAVRRKYAELEHGLAVNYMKKV
                     KEVFDPKGIMNPGKLF"
     gene            2694..4004
                     /locus_tag="P5549_04010"
     CDS             2694..4004
                     /locus_tag="P5549_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRW12266.1"
                     /translation="MEFKELPPVKEQIMKCVRCGKCRSVCPTFAEIGNETAAPRGHVF
                     MVGMLRNGVVEPSQEVYDRLGNCLMCEACSVACPSGIDIHELNAAARSYIYEKNPSLG
                     KELIFDTMWTRPGMLKTSVRFMWLAQKTGLQKLGRSLGITKLLPGDLPKAEKILNSVP
                     YFPARSELDEVNPARGEKRYTVGYFLGCATNLFYPEVAKATVDVLTRNGCEVIIPNDT
                     KCCGLPQIANGKTETARELAVHNVKIFNAYDFDYIVTDCASCSSALSHKNMEYLLGGL
                     KIEDQAFAFSEKVMDLTKFLIDVLDVHPPVNAKAKKMKVTYHDPCHLANAQGIKEQPR
                     ELLRRIPGVELVEMKEANRCCGGSGTYSLTHYDISMKILDRKIDHAMLTGASKLATCC
                     PSCTMQLRYGVERSKWQADVVHPVVLLSQSYRQAKAVQAQEKVK"
     gene            4001..4732
                     /locus_tag="P5549_04015"
     CDS             4001..4732
                     /locus_tag="P5549_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HRW12267.1"
                     /translation="MNKTLNKKIFRDVRRANLKYKMVETGDRIAVGLSGGKDSTALLY
                     FLSMLQQYTPLDFTLVPIYLDLGFDNDDSPLQQVCADLQLPLLVEKTNIGTIVFDIRQ
                     EKNPCSLCSNLRRGALNRVAKRNNCNKVALGHHADDAVDTLLMSMIFEGRYHLFKPVT
                     YLDRIDITVIRPMILVSEQDIKRFCSSIAFLPVENRCPADGLTKREEIKTILADIESR
                     CPGARQRILTSIANVDANSFWTAAP"
     gene            4864..5490
                     /gene="lexA"
                     /locus_tag="P5549_04020"
     CDS             4864..5490
                     /gene="lexA"
                     /locus_tag="P5549_04020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRW12268.1"
                     /translation="MDMEKINTLKRRPRMVFEYIQSYCKEHGYPPSVRDIGKAVGLKS
                     SSTVHMYLVQLEELGLIRRDPARPRAIVVNEPEEVANLEMSIRQIPVVGKVAAGIPIL
                     AQENIDNYMAVPVDLLGQGNYYILKVQGDSMIEAGIFDNDYVIVHEQRDAANGEIVVA
                     MLEDEATVKRYYKMPDHIKLQPENSAMEPIIAKEVSILGKVAGLMRRM"
     gene            complement(5585..6895)
                     /gene="glnA"
                     /locus_tag="P5549_04025"
     CDS             complement(5585..6895)
                     /gene="glnA"
                     /locus_tag="P5549_04025"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRW12269.1"
                     /translation="MQKCKEENVQFIRLQFTDIMGVFKAISITVDQLEKALDGELMFD
                     GSSIEGFVRIEESDMYLKPDPDTFAILPWSNPKSSGKTARIICDIYDPDDTPFKGSPR
                     YILQKALQEAAELGFAMQVGPEPEFFLFNLDENGQPTLKTNDKASYFDLPPVDMGEEA
                     RREMVEVLQTLGFEIEAAHHEVAPGQHEIDFKYTDALKTADNIMTFRVAVRIVAQNHG
                     LHATFMPKPVFGIAGSGMHMHCSLFKDGENIFYDPNREDGLSDTCRYFIGGVIRHARA
                     ICAITNPSVNSYKRLVPGYEAPVYIAWSYKNRSPLIRIPARRGIGTRIEVRNPDPSCN
                     PYLGLAVILKAGLDGIKNQIAPPDPVEQNIFEMNLADRKKQGILSLPENLYEALDELG
                     KDEIIKEALGEHVYSRFVAAKMKEWENYSSRVYDWEIDEYLAKF"
     gene            complement(6979..7458)
                     /locus_tag="P5549_04030"
     CDS             complement(6979..7458)
                     /locus_tag="P5549_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRW12270.1"
                     /translation="MSTGAVVVAIIVILIGIVGIFIPYIPGIPLIFIAILAYGWYEGF
                     HQISAYYIALMAALTVLALVVDYLSATLGAKHFGSSKYGIMGALLGTLLGIIIFAPIG
                     IFVGPFLGALAGEMLSGKDMDTAVKTSLGTIAGLFTGIAFNLVLGLVMFISFLIIIF"
     gene            complement(7525..8757)
                     /locus_tag="P5549_04035"
     CDS             complement(7525..8757)
                     /locus_tag="P5549_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HRW12271.1"
                     /translation="MDALNLIRQAEKDMHEQFAYYEAIAYDNQAKVLRAFQEHRVREG
                     HFQFSSGYGYGDLGRDELESIYAQVFGADDAMVRAQIVSGTHAISACLLAVLRPGDEL
                     LSLTGSPYDTLSNVIGVHHPHRGSLIERGIKYREIALLPDGDVDLEAIRLTVSPQTRM
                     VLIQRSRGYSLRPALTAEKITQVIAAVRQANPATIVFVDNCYGEFTDRLEPTQLGADL
                     MAGSLIKNPGGGLVPSGGYILGRRDLVEEVSFQITAPGLGKELGASLINNRLLYQGLF
                     MAPHTVLQALKTACLLAWVGEHFACRVFPRWQEARGDIVQAVQMNHAAEVIRFCQIVQ
                     SASPVDSDVTLEYGDMPGYADRVVMAAGTFVQGSSIELSCDAPLREPYTAYFQGALSY
                     EHGRYVVQQLIEQLLGQR"
     gene            complement(8774..9685)
                     /locus_tag="P5549_04040"
     CDS             complement(8774..9685)
                     /locus_tag="P5549_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW12272.1"
                     /translation="MAISMRYAQPGNDGPAILSPCNPEEHAFLQNDAFRELEKMIGLK
                     PVKKTIAEISAYAMIQTRRSQQSLKADPTAMHMVFRGNPGTGKTSVARLLGGIFREIG
                     ILSKGHLLEVERADLVGEYIGHTAQKTREVLKKASGGILFIDEAYTLAQGGSKDFGQE
                     AISTLVKAMEDQRHDLIVILAGYCLEMDAFMLSNPGLRSRFALQINFPDYESDELFNI
                     ALQMYNERDYELSSRCRWRLKCIMEEFVKGHHPHSGNARYVRNLVERSIRQQALRLVD
                     RAYLSRKDLMTVEEKDLAVPGTQPLSY"
     gene            complement(9759..11660)
                     /locus_tag="P5549_04045"
     CDS             complement(9759..11660)
                     /locus_tag="P5549_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRW12273.1"
                     /translation="MKIAFTTFLLVEMLIFHFIPFALFDKQEKDYVASHKIVYVAGHP
                     DFYPLEYYDRQLREYAGIVPDLYRQVAADSGLVFQYINTDQANAAELAQNGQVEVMLA
                     LTSLPTNTVSDRVNFSVDYSGKPIDLTFLFTYAAAPELVSIFAKSLQNMTDGEKEAIL
                     QHYLSGKKSGLSMTFIVLAGLLLLIILGWGMHLAKKLREQKKLNLTNRNTDTFTGLSS
                     LDGLDYHYQQLINDKNRSLYAVIIIDSSMKGIGRLYGIDEVYSLLKHIAGTIRQSLQT
                     DELAARVSEYTFCVVKPSHSQAEMIAWLEELNRAIIDYSPASEKGYAIQMHYGIYFLR
                     HGDQLADKALYNAIQAKEYARLKRMQYAVFDDEVLREANEYQAFEQEVLSGLENKEFV
                     PFFQPFVDIRTGKVIGAEALARWESRTRGLLRSASFVPQLEKHNLISELDFQIFEKSC
                     QWLSQRHQSGKTMTRLSCNFSRQTFQHKDFIGRLTAILDEYPFPHQCLAVEITESAMI
                     VDTEYAEQNIEKLNKLNIGLFLDDFGVGYASFKDLCEYPFDCIKLDKSLIDNLDQENW
                     RVLVNGLIYISHNMNFKIICEGIEREEQVAKLRALNCDIAQGNYFYRPLSQSLFETLL
                     DDALLPTVY"
     gene            complement(11687..13087)
                     /locus_tag="P5549_04050"
     CDS             complement(11687..13087)
                     /locus_tag="P5549_04050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRW12274.1"
                     /translation="MDFIRIVIEYTGIFFIAYLIGYSTFLFLSVIVGGTVLFDKRKKE
                     RLKNVLNHDYYMPITIIVPAYNEEVTVVDSVKSLLSLDYQLYEIVVVDDGSSDSTSQK
                     MIEHFDMKQIRQPINRAVKCKPVRAIYSTDQQKVPITLIKKENGGKADALNMGINASK
                     FPYFICMDADSVLQQDSLKEIMNTILEDNNIIAAGGLVRPSNGLKIMEGRVINYRLPP
                     KILVAMQTLEYERSFLASRLLLDQFNGNLIISGAFGMFKKDIVIDAGGYDHSTIGEDM
                     ELVVKLHVFCRANNRPYLIKYAPDAICWTQVPERLHDLTSQRRRWHLGLFQSMWKYKE
                     VFANPRFGAISWISYFYFLVYELASPFIELFGIITIVLAFLVELINVKFMIMFFLIYV
                     IFGVILTTTAFINRIYTQNQTLYLTDIIKATGLCVIEISVLRFIMAWVRITAFIGYNR
                     RKTDWGKIERQKINLR"
     gene            complement(13100..14119)
                     /locus_tag="P5549_04055"
     CDS             complement(13100..14119)
                     /locus_tag="P5549_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRW12275.1"
                     /translation="MIDQRQYIKRSQKLNRLLDKQLERLEKGLPVTRQHQQYLQKELP
                     RVKYLMAFAELLDRKAVGQQDYFDEYLRTLSPIFEFLARKYQQQKVISKSYFAHVLAH
                     PRLARNLNTDALAKILFFYLQEDSIHCQANVMKALFSFGDVSSVMRGIRILDRLEIYF
                     HGKLLTDGLLTFQGSHQELSQTLWKNLFQFRIPIQVALLDYLRFTTGAYQEELAVLMG
                     DASVHPEVQFAIIRYFTRYPFPAAREKLLAFLQIKDKDRWQYPALAATALAAYPDQEV
                     AGALQKALSSSNWYIRYNAAKSLTSMQMDLAEIAQVINGDDRFAREILQYFMEYNPNF
                     KPSMG"
     gene            complement(14216..16363)
                     /locus_tag="P5549_04060"
     CDS             complement(14216..16363)
                     /locus_tag="P5549_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12276.1"
                     /translation="MKKYIAVCFGLLMIYFIYQYLTLSLGLYYDFHPEQPVNAMAKTD
                     RARIYLSQGNRLEQLVVKGVELESVKPGCFANDYEVDEPTYLRWLQLIGEMGANTVKV
                     NHIMDEAFYNAFYDYNQKAAEPLYLLQSIAVRDYEQNAANGAYSEAFFENLITNCRIA
                     VDVIHGQRLITLNKGAGIGNYRKDVSPWTLGFIVGTTWFPYTIAYTDHHQEPERHYQG
                     KYVFATAEASAFENMLAEVMNEILSYEAGKYKQQRLVSFINEPTADPFEYLYNVNIQI
                     DKVAQVDANHIQLTDAVASGFVVSYRMYSYYRYFTECLADSERLRLAEYLSQVDVNTI
                     YDGYINMLNLYYDYPVLVTSYGFSTARGAESKVEERLTEQAQGVLLADTYEQIVQAGC
                     CGAVISAWQDDWSHAAWNTQHAVDLNQVKNWHDAQSLVQFYGLLSFDAGAQESVCYVD
                     GEPGEWNEDDIIIAEPTLSLSLKYDEQYIYLLVRKKDLRQDEMLYLPIDTTPQSGSNY
                     SAQYKVRFQRPADFLMLINGQNNSRVLVHRRYDVMRAMYEKSTTGLEPYEFVPDKALP
                     VFDKIRMVLKKQYDPGVDIIQLSDQERFIHNLYQTIETGALTHGNANPKSSSYNSLAD
                     FFYGDDLIEMRIPWQLLNFSDPSAMMIHADYYAHYGVEYMPIKAMYIGVGDAVTGSQR
                     IAMGRVKLKGWAGEASYHERLKPAYYIVQQRWQ"
     gene            complement(16386..17867)
                     /locus_tag="P5549_04065"
     CDS             complement(16386..17867)
                     /locus_tag="P5549_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRW12277.1"
                     /translation="MKPKIILMAAVMIMILTTTFITVMPKSDQRRVHQHREDTVQKCV
                     HQNEVFCTHLPIVNISTGGQKIPGEPILNELGNTIALETGDNGETMIIAEISVIDNAR
                     ANNHIGDTKAVDASAWLRIRGNSSRIFDKKSYAIRFIDTHGYDRELAVMGMPAHDEWA
                     LYGPFLDKTLLRNYLGMNISAEVMGYAPNVRFCECFLDGRYQGVYLFMETVARSENRV
                     NLTGYDPDHVASSYIVRMDEYEHNERSLDNFTYYTLNNKYNTGFTIVYPPRLLMTPET
                     RTFIEDDISQFEKALYSFDFKDPARGYRAYIDVDSFVDYYVLQEFFLNNDMCSRSTFL
                     YKDIRGKLHMGPVWDYNNAFNNFIPLDFDGTDLQFIDRTWYQMLLRDEYFVNKVIARY
                     KALRKSYLSDEYLLNYIDETTNYLGGAIDRNYMVWGYTFEREHQKPGVSRRPIELNPT
                     SYEEAIDQLKTIIVKRGEWMDKHIDILQQNCHGSKIKHFLPLP"
     gene            complement(17881..18558)
                     /locus_tag="P5549_04070"
     CDS             complement(17881..18558)
                     /locus_tag="P5549_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRW12278.1"
                     /translation="MKQDLLQIFQQSSELGWESIVLHIVVAILIAIVIFLSYRLSHSG
                     TIYSEKFNISLVILTILTTTVMTVIGNNIALSLGMVGALSIVRFRTVIKDSRDTVYIF
                     WAIIVGICCGVGHYLVAALGSCAVFIILLVFGKIRNDNRILLIIRAARNIDRKIEAII
                     FDYFDDKAQLRVKNTTEANVEFIYELTKKVVDKTITREKNITEILYELGSVEYVNLVT
                     QNDEISS"
     gene            complement(18573..19268)
                     /locus_tag="P5549_04075"
     CDS             complement(18573..19268)
                     /locus_tag="P5549_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRW12279.1"
                     /translation="MNTVMRQEKKFLITIEDAIKHSRYLENFMMQDSHNGTHGYMIRS
                     LYFDTLTDRDYFEKLDGVEVRRKVRLRNYDPRSDFAVLEMKQKHGTQQLKRSLRMNRA
                     DAAEMCRGNYSPLLTYSDPFAAECFGFMHMYCYRPKTIVQYNRKAFIAQENKIRVTFD
                     QNITATESCFDIFAENLTMYPVFNPFNVVLEIKYNGFFLSYIREFINRINRSELAVSK
                     YCLGRSVALNYIF"
     gene            complement(19351..19677)
                     /locus_tag="P5549_04080"
     CDS             complement(19351..19677)
                     /locus_tag="P5549_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12280.1"
                     /translation="MSNCIKCGREIADKSFQLTLYCDACYRALHTRHCITCGKEITDK
                     SFQLTRYCDACLQESKRRHCEKCGKEITDKSFQLTKYCSECWKETHQRRRIRHKSAHK
                     KTHDKK"
     gene            19869..20921
                     /locus_tag="P5549_04085"
     CDS             19869..20921
                     /locus_tag="P5549_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRW12281.1"
                     /translation="MSFEIVYHLRRALEKLPQLHYGRKLSYIPSAYDPRDYRYAKMIV
                     AAQPGATLPIDYRDQLPPVFDQEDRGTCVACAAAWTVKAYEEIKQADYPSEGLSASFL
                     YSMCKENDGLPCLEGTQPKVAMQVLRKYGICPEAIMPYAQLSELPCPKVPGIPQKAME
                     TASPYKIQTYAQLCSTSDSDRHELLEVIRQALQNEGPFMLALLVCENFQPDDDYELPL
                     PAGLLRGGHAVGIVGDLPERECLILRNSWGSSWGEDGYAYLPYSWLNHKICGSWSVSE
                     AWTATDMTLLPQARRIVVTPGLKTIKVDGKRAAMNNTAFFSKSAPHQNALQELVRNMG
                     YRLDWEGKQAVFTRML"
     gene            complement(20996..22492)
                     /locus_tag="P5549_04090"
     CDS             complement(20996..22492)
                     /locus_tag="P5549_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HRW12282.1"
                     /translation="MFKRQQHASLSRNYNDNLDLFKKELGEGESFDVIVREIKVVGLK
                     AALICFDGLVKDDILTEVMESLFVLRKEKYFENTMEQLLHRGIPYIELSTEGKIDELL
                     YAVLSGSILLIVDGSSEGFIIDARTYPARGPEEPDIERVVRGSRDGFTETVVFNTALI
                     RRRVRDPKLRVELLRVGKRSKSDVAVIYIKDIANPDLVETIKERIGAIKIDGIPMAEK
                     SVEELIIGGNTWNPFPKVRYTERPDVASLHLLEGHVLIVVDTSPSVIIAPATFFHHLQ
                     HAEEYRQNPSVGVYIRWIRFAAILASVFLTPLWLLAALSPDLLPAGLDFIGPKKTGNV
                     PIFLQFFLAEISLDLLRMAAIHTPAPMATALGVIAALLIGDVAVQIGLLIPETILYTA
                     MAAIGTFCTPSYEMGMANRLARLFILICTGLFRGPGLLARTLLVLTVLAFTKSFQVPY
                     LWPLIPFHWRALKTILVRSPVPVQNIRPEILRPRDTRRQAAPAMKKKP"
     gene            complement(22789..24582)
                     /locus_tag="P5549_04095"
     CDS             complement(22789..24582)
                     /locus_tag="P5549_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12283.1"
                     /translation="MANKFASRRNLAFLLEEVFDAASLNRIEYFQDHNQETFKMIIDT
                     AMKISTDMMFPYFKEMDTDPPYWENGNVHVNPAIKDFLKEMGEGGWIQAEKPYEIGGQ
                     QLPLTIKAAAYFLFGAANYSMNVYQLLSAGAANLIYSFGTPEMKDLYLEKMYAGEWQG
                     TMALTEPDAGSSLGDLTTTAEPTDQGYYLIKGKKIFISAAKNDVTENVVNLMLARVKG
                     APAGVGGISLFVVPEKRINDKGELEYNDVFCAGIEHKLGYRGSPICQLSMGDEKDCRG
                     WLVGQEGKGLAHMFKMMNEERINIGIGAISKISGAYYASLEYARERKQGRNPDQKDPL
                     TPMVPLIEHADIKRMLLFQRSVMEGALCLALQVSLYQDYENVNIDREENGLLVDFLIP
                     ILKSYPSEMGVLATSAAIQILGGYGYCGDFPVEQYFRDIRIDPIHEGTTGIQGQDLLG
                     RKVSMHGGKAFALYISEVEKTLDQAQLHPQLAPCVKALAEAVQLMKDLTAQLLAKKAS
                     LSKEAFLADSVLYLDFMGQIAIAWQWLKQMTVAQTALQQGAVDPAFYQGKIFTGRYFF
                     EYELTKIDSLAIRLRSDDRVTVEISDPYFED"
     gene            complement(24713..25651)
                     /locus_tag="P5549_04100"
     CDS             complement(24713..25651)
                     /locus_tag="P5549_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW12284.1"
                     /translation="MRGTNYKNAFSLTTNPCNLCPRMCGIDRSSQTGFCQSSSQIKAA
                     RAALHFWEEPCISGTRGSGTIFFSGCTLRCCYCQNHQISSAGFGQEISVERLAEICME
                     LQTQGAHNLNLVTATQYLDQILPALDLIKEQLHIPVVYNSSGYERVEVIQALAGYVDV
                     YLPDLKYYSDELAWQYSHARDYFSAATAAIQAMTAQTGGLVFDEEGMLQKGVVIRHLV
                     LPGARQDSFAILQWLSENLPPDKFMLSLMSQYTPVYKSVEHPELNRRITSFEYETVVA
                     EAVRLGLTSGYMQERSSAREEYTPPFDLEGIIKRLL"
     gene            complement(26032..26484)
                     /locus_tag="P5549_04105"
     CDS             complement(26032..26484)
                     /locus_tag="P5549_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12285.1"
                     /translation="MQNNSPNLGELLKALKTNFMTMAELERAGRHDELIIRKGPWGYD
                     YRFQQQYPEHPMSGGRYIRIKAADQIRDIARDRREHFTTEVLEELHLSLFDCCATVRL
                     SITEALYYVGDSSSISFLTELAAWEKESNMVKLQTIDTLRKLERLYLK"
     gene            26710..29019
                     /locus_tag="P5549_04110"
     CDS             26710..29019
                     /locus_tag="P5549_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRW12286.1"
                     /translation="MQEQSKKTTSPAEEQVFGCMCVGNCQSRCRLFVHVRDGKVVKTS
                     MAPFPDPRYNRVCLRGLSHVQQIYNPNRIKYPMKRVGERGEDKWERISWEEAIATIAE
                     KWKTYQAEYGPQSVAFSTISGDMAMINGLYSAQRIAQTMGATLTEYSLDMADTYGLGR
                     VIGNMGTFNQSNDPADLANAKTVLVWAANPTESHPHSWRYIMQAQEAGAKVVVIDPRY
                     TTTAAKADRYIPIRPGSDAVMALAMAETIIAEQLYDAEYLRYHTVAPFLVREDTKRFL
                     RQSHLTGIAPEAGQPDPYIVWDKEAHAGKVLAEVPDPQLEGTYTIDGIKVSTAFTVLK
                     EFLTDYTPEKATGLCDVPTETIRETARLYATNTPASIFNGFIQYDNGLQTGHAWGILV
                     SLTGNIARNGSSVGHCGMAFYSCNFIKYLFPRGKNSPTIPWLLLPEVLKTGEFYGKPH
                     PIKSMYFLCSNAVTNNTNQNRIINEIFPAIDFIVVADIVHTDTTKYADLILPVAHWFE
                     MLEFVQGPDHPYSVLSEKALEPAFESKSNTDIFRMLSAALGFGEVYNYTDEEFIQMLI
                     DTDLSRQLGLTWERLKEEKVIQTLPSCWVNWAGNKFPTPSGRLEFYLEDPQPRLDCGI
                     PIDQDKERLPVFTPPIEAWPDNPLKQKYPLIYHTERVRWRMHSQWSSTPWLRELDPEP
                     TVKINPSDADSRGIKTGDMVEIFNDRGHVVLKAILSEGEPPGMVSVPKGWHRDQFKAG
                     SYQELTTDHVNYSSYNQSFYDVLCEVRKV"
     gene            29038..29694
                     /locus_tag="P5549_04115"
     CDS             29038..29694
                     /locus_tag="P5549_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW12287.1"
                     /translation="MGNKRLVMLIDINRCIGCHACAVACKANNNLPNEIWWNQVLTHG
                     GKSMDTPRGQYPNLQLQFLPLACQHCDNPPCVKACPTGATYQADNGIVMMDYDKCVGC
                     GSCILACPYKYVRQFNEKEPQYMLDFPTGNEGVPAQQKGTVSKCTFCHTLVAKGETPA
                     CIEVCPVNARYFGDLTDKESTVFKVLQENQHLQLLTERGTNPSVYYLTGRKPQDGHKQ
                     "
     gene            29713..>30212
                     /locus_tag="P5549_04120"
     CDS             29713..>30212
                     /locus_tag="P5549_04120"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HRW12288.1"
                     /translation="MLRKEWPLITNTILAQLAAGLFIFLAWDRIVLSRESLQLALTST
                     AQGLAWTGPVIVLAMLLSLFHLGKPFRAYRAMANLPVSWLSWEIFFSGAFLLLWIIFM
                     VFESSDSIALPLMVAAGLVGLLNVISMSNIYSSTGRPGWKGTGTYLDFLGSTLVFGSV
                     GAAFFLS"
BASE COUNT         7599 a         7758 c         7099 g         7756 t
ORIGIN      
        1 gcctggtaca tgttgatatc cttgggatag cctccacagc tggcaattac aatgtcagca
       61 ggagcatcta ttcggagtac gctcatctgc tcggcatatt gcactgcggc cagccaggct
      121 gcttcaacat ccccactggc acagaaggcg atgttatgat gactgtgcaa gaccacattg
      181 atgataaaat caacgccggc cttgcgggct gcttccaaca tcaaatcact aaccggatta
      241 ttttcatagt tgcccaaaca agctcgggga tcactcatca ttttatgatt ggcttcgatg
      301 gccgcgcggg ctgctacacc aggcaggatt gattttctgc cgccggaata accggcaaaa
      361 taatgcagtc ccaccacacc cgtagtaatc aacaggtcgg cttcagccac ggtgcggttg
      421 ataatcagtt ccatgccatt ggacaggtaa ccaaggaatt tgagatcgtt gtcacagtca
      481 tggttgatga ttcggtattt acggcagatc cctgcgccaa aaatcgccaa gttgtcttct
      541 tgggtatgaa cgcgatggat gccggtagcg atcaccagcg tgactttttg atccgggatg
      601 ccagcctgtt cgatttctct caacaaaggc ggcaatatca attcgtaagg cgtaggccga
      661 gtgatatcgt tgactacgat gaccgcgctg cgggcctctt tggcagcgat gatttcccgc
      721 agggccgggc ttgccacagg atgggccagc gattcctcca ccatttccat gccgcctccg
      781 gcgctttgca gatgacgggg ctgcaatatg gctgctaaat tcttttcgtt cagctgcagt
      841 tcaatatggc tgcggccata agctaattta atcacgattc cttccctgcc gtttcttaca
      901 ttatttattc attataccgt aagtgcttgt ctattatcat ctataactgc cacgcaagtt
      961 ggggacggtt cttgacttgc gtttttcgcc cattattacg caagttgcgc aagttgggga
     1021 cggttcttgc ttgccttttc aatctatttt agcaagctta ttctgcggtt tttaccttcg
     1081 cttgcttggg gccggcttct tgtcatacca gcattaaatg ccttgccatt gccatgtata
     1141 caaactttac atcagaatat aaagtcgatt atgatataat atcgaggtat aaaccctgat
     1201 atgattatac ttttgaggag tgaagctatg gattccgcag cgatcagaaa agaactggta
     1261 aaattgctgg gtaaagataa ggttctaaca gaaccactgg atttgatgta ttattcctat
     1321 gacagttctt ttttaaccca aatgcatcct gctgatcctt atgcggtggt ttttccgaaa
     1381 agtaccgcag aggtgcagca agtgatgcgt tttgcctatg aaaacaatat caatgtcatt
     1441 ccccgcggcg ctggcaccgg tgaaaccggc ggctgtattg cgactgaggg cggaatcgtc
     1501 atggagctgt ccaactggga tgaaattgtg gaaattgatg ccaagaacat gcaggtcatt
     1561 gtcagaccgg gcgtcattca tgccaaatta aacgaagaac tggaaaaagt gaatctcttt
     1621 ttcccgcctg accccggcag cagccagatg tgtacagtgg gcgggatggt cgccaacaat
     1681 gccagtggtt tgcgggcggt taaatacggc actaccgagc aatacatcct ggggttggaa
     1741 gtcgttactc ccaccggtga catcatctac accggcggca tgaaagcccg ctgtataaag
     1801 aatgtttcgg gactgaatct gaccaaactg atgatcggtt ctgaaggagt tctggggatc
     1861 gttacgcaaa tccgtctgcg ggtctggccc aagccaaaag ctcgcggcat tgccatggct
     1921 gtgttcgatc agcttgatga tgctcctgcc acggtactgg aaatatacca gggtggcctg
     1981 ttgccatcgg gaatcgagat actggacagc tcggccatca aagctgttaa tgaattcaag
     2041 ccggaaatca atctgcctga ttgtgaagcc attttgctct tcgaagtgga tggcaatccg
     2101 cccagtgtgg aatatgaagg cgaacagatt aaggaaatcg ctggacgcag agcccgtttg
     2161 gtagaatggt cgaccgatac aaaacgtatg gcccaattat gggaaggccg cagcgttact
     2221 gcatctgccg cttcccgggt cagagctgac ggcacccggg tattcgccgg cgaagatatc
     2281 agcgtccccc tggccaaagt ggcagataca ttgagagcta ttcgtcagtt ggctgccaaa
     2341 catggcattc aggttgttaa ctacggccac atcggtgatg gcaatgtgca taccgccccg
     2401 gtgattaacc ctgaaatccc ggatgaagtg gaacgggtca tgcaactggt ggatgagatc
     2461 cacctgctgg cgattgaaat gaatggttcc accaccggcg aacacggtgt cggcgccgta
     2521 cgccgcaaat atgcagaatt agagcacggc ctggccgtaa attatatgaa gaaagtcaaa
     2581 gaagtctttg accccaaagg catcatgaat ccgggaaaat tattttaaac taaacgcaag
     2641 tcaagaaccg tccccgactt gcgcggctaa tcctccagga aggaagaata tcaatggaat
     2701 ttaaagaatt acccccggtg aaggaacaaa ttatgaaatg tgttcgctgc ggaaagtgcc
     2761 gcagtgtttg ccctacattt gcagaaatcg gcaacgaaac ggctgctccg cgtggtcacg
     2821 tcttcatggt cggcatgctg cgcaacggcg tggtggagcc gtcccaggaa gtgtatgacc
     2881 ggctgggtaa ctgcctgatg tgcgaagcct gttccgttgc ctgcccatcc ggcatcgata
     2941 ttcatgagct taacgccgcg gcgcgttctt atatctatga aaaaaatcca tcattgggca
     3001 aggaactcat ctttgatacc atgtggaccc gtcccggcat gctcaagact tcggtacgct
     3061 ttatgtggct ggcacagaaa accggtttgc aaaagctcgg ccgcagtctg ggaatcacca
     3121 aattactgcc cggcgacctg cccaaggcag aaaaaatact gaattccgtg ccttattttc
     3181 cagcccgcag tgagcttgac gaggttaacc ccgcccgcgg agaaaaacgt tatacggtcg
     3241 gatacttcct gggctgcgcc accaatctgt tctatcccga agtggccaaa gccacggtgg
     3301 acgtattgac ccgcaacggc tgcgaggtta ttatccccaa cgatacaaaa tgctgcggac
     3361 tgccccagat tgccaacggc aagaccgaaa ccgcacggga attggcggtt cataacgtga
     3421 aaatctttaa tgcatatgat tttgattata tcgtgaccga ctgcgcttct tgttcctctg
     3481 ccctgtccca taaaaacatg gagtatttgc tgggcggctt aaagattgaa gatcaggctt
     3541 tcgctttttc cgaaaaagtt atggacctga ccaagtttct aattgatgta ttggatgttc
     3601 atccaccggt aaatgccaag gccaaaaaaa tgaaagtcac ctatcatgat ccctgtcatc
     3661 tggccaatgc ccagggcatc aaggaacagc cgcgcgaatt gctgcgccgc atccccgggg
     3721 tggaactggt agagatgaaa gaagccaatc gctgctgcgg cggttcaggc acatacagct
     3781 tgacccacta tgatatttcc atgaaaatcc tcgaccgcaa aattgaccat gccatgctga
     3841 ccggagccag caagcttgcc acctgctgtc ccagctgcac catgcagttg cgctatggtg
     3901 tggaacgcag caagtggcag gcggatgtcg ttcatccggt ggtcctgctg agtcagagtt
     3961 accgtcaagc caaagctgtc caggcacagg aaaaagtaaa atgaataaaa ccctcaacaa
     4021 aaaaatattc cgcgatgtcc gccgggccaa tctgaaatat aaaatggtgg aaaccggcga
     4081 ccgcattgcg gtgggtttat cgggcggcaa ggatagcacc gccctgctct attttctcag
     4141 catgctccag caatataccc ccttggactt caccttggtg ccgatatatt tggatttggg
     4201 cttcgataat gatgacagcc ccttgcagca agtctgtgct gatttgcagc tccccttgtt
     4261 ggttgagaaa accaatattg gaaccattgt tttcgatatc cgtcaggaaa agaatccctg
     4321 ctcgctttgt tccaatctgc gccgcggcgc gctcaaccgg gtggccaaaa gaaataactg
     4381 caacaaagtc gccctgggac accacgcaga cgatgctgtc gataccctgc tgatgtcaat
     4441 gatttttgaa ggccgttatc acctctttaa acccgttact tatttggacc gaatcgatat
     4501 cacggtgatc aggccaatga tcctggtatc cgagcaggac atcaaacgct tctgcagctc
     4561 aattgcattt cttccggtgg aaaaccgctg tccggctgat ggtttgacca aacgggaaga
     4621 aatcaaaacc attctggcag atatcgaatc gcgctgccct ggggccagac agcgtattct
     4681 caccagcatt gcaaatgttg acgccaacag tttctggacc gcggcccctt aattctcctt
     4741 ggtcagttca tttattttga catagaagtg tcatcgttac ctcgttttgc caactttttt
     4801 gtctagaaca attgtttttt atagaacatg tgttataatt taacaaaaac caggaggggt
     4861 cttatggaca tggaaaagat taataccttg aaaaggcgtc cgcgaatggt gtttgaatat
     4921 attcagagtt attgcaagga gcatggttat cctccgtcag tccgggatat cggcaaagcg
     4981 gtcgggttaa aatcaagctc cactgtacat atgtatttag tccagttgga agaactggga
     5041 ttgattcgcc gcgacccggc ccgcccccgg gcaatcgttg tcaatgagcc ggaagaagta
     5101 gccaatctgg agatgagtat tcgccagatt ccggtggtgg gtaaagtcgc tgccggcatc
     5161 cccatcctcg ctcaggagaa tatcgataat tacatggccg tgccggttga tctgctcgga
     5221 cagggcaatt attacatatt aaaggttcag ggcgacagca tgatcgaggc tggcattttt
     5281 gacaatgatt atgtcatcgt tcatgaacaa agggacgcag ccaacggtga gattgtcgtt
     5341 gcgatgctgg aagacgaagc tacggtaaag cgctattata aaatgccgga tcatataaaa
     5401 ttgcagccgg aaaacagtgc catggaacca atcattgcca aagaggtcag tatattaggc
     5461 aaggtagccg gtttaatgag aagaatgtag aaaccaagtt acgcaagttg gggacggttc
     5521 ttgcttgctt tctgcccccc agaaagcaag caagaaccgt ccccaacttg cgtctaattc
     5581 tattctaaaa cttggccaga tactcgtcga tttcccaatc atagacccgg gagctgtagt
     5641 tttcccattc cttcatcttg gcagcgacga agcgggagta aacgtgttct cccaaggctt
     5701 cttttataat ctcgtccttg cccagttcat ccaacgcttc gtaaagattt tcgggcaggg
     5761 acaggatgcc ttgttttttt cggtcggcca gattcatttc gaaaatattc tgttccactg
     5821 gatcaggtgg agcgatctgg tttttaattc catccaagcc ggcttttaaa atgaccgcca
     5881 gacccagata cggattacag ctgggatccg ggttgcgcac ttctatgcgg gtgccgatgc
     5941 cgcggcgtgc cggtatcctg atcagcgggc tgcggttttt gtatgaccag gcaatataaa
     6001 ccggggcttc ataacccggc accaaccgtt tataggaatt gaccgagggg ttggtgatgg
     6061 cacaaatagc ccgggcatgc ctgatgacgc cgccgataaa atagcggcag gtgtcactga
     6121 gtccgtcttc ccgattggga tcataaaaaa tattctcgcc gtctttaaac agggagcaat
     6181 gcatatgcat gcccgaccct gcaatcccaa agaccggttt gggcatgaag gtggcatgca
     6241 gtccatgatt ctgggcgaca atacgtaccg ctacccggaa ggtcatgata ttgtccgctg
     6301 tctttaacgc atcggtatat ttaaaatcga tttcatgctg ccccggagcg acttcgtgat
     6361 gagccgcttc gatttcaaat cccagggttt gcagcacttc caccatctcg cggcgggctt
     6421 cttctcccat atccaccggt ggcagatcaa aataactggc tttgtcattg gttttcaacg
     6481 tcggttgtcc attttcatcc agattaaaaa ggaaaaattc cggttccgga cctacttgca
     6541 tggcaaaacc caattcagcc gcttcctgca aggctttttg taaaatataa cgcggactgc
     6601 ccttgaacgg agtgtcgtcc ggatcataga tatcgcagat aatgcgagcc gtttttccac
     6661 tcgatttggg attgctccaa ggtaaaatgg caaacgtatc ggggtccggc ttgagataca
     6721 tgtcagactc ttcgatgcgc acaaagcctt cgattgagga accgtcaaac atcagttcac
     6781 catccagggc cttttccaat tggtcaacgg taatcgagat agctttaaat acccccatga
     6841 tatctgtaaa ctggagtctg atgaattgca cattttcttc cttgcatttt tgcataatgt
     6901 attctttatt cacgccaatg ccccctattt gctgctttct gatttgatta ggttagcata
     6961 taaatctctg ctgtgcaatt aaaagatgat gattaaaaaa gaaataaaca taaccagccc
     7021 caggaccagg ttaaaggcaa ttccggtaaa caacccggca attgttccca gcgaagtctt
     7081 tacggctgta tccatgtcct tgccggacag catttccccg gccagagctc ccaggaatgg
     7141 ccccacaaaa atcccgattg gcgcaaaaat aatgatcccc agcaaggtcc ccagcagtgc
     7201 gcccattatt ccgtatttgc tggaaccaaa atgtttggct cccagggtgg cagaaagata
     7261 gtcgaccacc agcgccagaa cggttaacgc tgccatgagg gcgatgtaat aggcagagat
     7321 ttgatgaaat ccctcatacc atccataggc cagtatcgca ataaaaataa ggggaatgcc
     7381 cgggatatac gggataaata tccctacgat cccaatcagg ataacgatga tcgccacaac
     7441 aactgctccc gtactcatgt ctatgacctc cttaatttat cgattcatct ttgctttgta
     7501 ctcggtaaac gttggcagaa ctccctatct ttgccccaac aactgctcga tgagttgttg
     7561 gaccacatac cgtccgtgtt cataacttaa agctccctga aaatatgccg tatacggctc
     7621 acgcagcggc gcatcacatg acaattcaat ggaagaacct tgtacgaatg tgccggcggc
     7681 catcaccacg cggtctgcat aacccggcat gtcgccatat tccaaggtta cgtcgctgtc
     7741 aaccggcgaa gcgctctgca cgatctggca aaagcgaatg acctccgcag catggttcat
     7801 ctgcaccgct tgtacgatat ccccacgcgc ttcctgccag cgcggaaaga cccggcaagc
     7861 aaaatgttcc cctacccagg ccagcaagca ggcggttttt aatgcctgca atactgtatg
     7921 cggcgccata aacaagccct gatagaggag acgattgttg attaagctgg ctcccagttc
     7981 ctttcccaag cccggtgcag taatctgaaa ggatacttcc tccaccaaat cccgacgtcc
     8041 caggatatac ccgccggagg gcaccagacc cccaccggga tttttaatca gcgaacctgc
     8101 cattaaatct gcccccaact gggtcggctc cagccgatcg gtgaattccc cgtaacagtt
     8161 gtccacaaat acaatcgttg ctggattggc ctgacgcacc gcggcaatca cttgcgttat
     8221 cttttccgcc gtcagcgccg gacgcaggct gtagcctcgc gaacgctgga tgagaaccat
     8281 tctggtctgc ggcgaaacgg ttaggcggat tgcttccaga tcaacatctc catcaggcag
     8341 gagtgcaatt tcacgatatt tgatgcccct ttcaatcaga ctgccgcgat gcggatgatg
     8401 cacgccaatg acattgctca gggtatcata gggactgccg gtcagcgaca acagttcatc
     8461 gccgggcctt aataccgcca ataaacaggc ggaaatggca tgggtgccag atacaatctg
     8521 ggcgcgaacc atggcatcat ccgcaccaaa cacctgggca taaatgcttt ccaattcgtc
     8581 ccgtcccaaa tctccgtatc cgtaacccga cgaaaattga aaatggcctt ccctgacccg
     8641 atgctcctga aaagcgcgca gtactttggc ctggttgtca taagcgattg cttcataata
     8701 tgcaaattgt tcatgcatgt ccttttccgc ttgccggatc aggttcaatg cgtccattac
     8761 tacatcactt tccttaataa cttaatggct gggtgcctgg caccgccagg tctttttctt
     8821 caacggtcat caaatctttg cggcttaaat acgcccggtc caccaggcgc agcgcttgct
     8881 ggcgaatcga tctctctacc agattacgca catatcgagc attgccgctg tgcggatgat
     8941 gacctttcac gaattcctcc atgatacatt ttaatctcca gcggcagcga ctgctcaatt
     9001 cgtagtcccg ttcattgtac atctgcaaag caatgttgaa aagctcatca ctttcataat
     9061 cgggaaaatt tatctgcaac gcaaagcggg aacgcaatcc cggattgctg agcataaatg
     9121 cgtccatctc cagacagtag ccggccagaa tcacgatcaa gtcatggcgt tgatcctcca
     9181 tggccttgac cagcgtggag atggcctcct ggccaaaatc cttgctgcct ccctgcgcca
     9241 acgtgtaagc ctcgtcaata aacagaatgc ccccgctggc cttttttaaa acctcgcggg
     9301 tcttctgcgc tgtatgacca atatattcgc ccaccagatc cgcccgttcc acctccagca
     9361 gatggccttt ggacaaaatt ccgatttccc tgaagatccc tcccagcaga cgggccacac
     9421 tggtcttgcc ggtgccgggg ttgccgcgga aaaccatgtg catcgcagtc ggatctgctt
     9481 tcagggattg ctggctgcgc ctggtctgga tcatggcgta ggcactgatc tcggcaatgg
     9541 tctttttcac tggtttcagc ccaatcatct tttccagttc gcggaaggcg tcgttctgca
     9601 agaaagcatg ctcttccggg ttgcaaggtg ataatatcgc cggtccgtca ttcccaggct
     9661 gggcataccg cattgatatg gccacaaaaa atcaccactc ttcgtatttc taggttacta
     9721 tatgccaaac agccaaaagt ggtgacatgg tatgcgggct aatacacggt cggcagcaag
     9781 gcatcatcca gcagagtctc gaacaggctc tgggacaacg gccgataaaa atagttgccc
     9841 tgggcaatat cacagttcag tgcccgcagt ttcgcaacct gttcttctct ttcaatgccc
     9901 tcacaaatga ttttaaaatt catgttatga gatatataaa tcagcccgtt gaccagcact
     9961 ctccaattct cctgatccag attgtcaatc aggcttttgt ccagtttgat gcaatcaaaa
    10021 ggatactcac acaaatcttt aaaggaagca taccccaccc cgaaatcatc cagaaacagc
    10081 ccaatgttga gtttgttgag tttctcaatg ttctgttcgg cgtattccgt atcgacaatc
    10141 atcgcgctct ccgtgatttc aaccgccaaa cattgatgag gaaacggata ttcgtccaga
    10201 atggccgtca accggccgat aaaatctttg tgctgaaagg tttgccggga aaaattgcag
    10261 gacagccgcg tcatggtctt gcccgactga tggcgttgtg acagccattg gcatgatttt
    10321 tcaaagatct gaaaatccag ttcgctaatc agattatgct tttccaattg gggaacaaaa
    10381 ctggccgatc gtaataatcc ccgtgtccgg ctttcccagc gagcgagggc ttccgccccg
    10441 ataaccttgc cggtacgaat gtcaacaaag ggttgaaaga aaggaacaaa ttccttgttt
    10501 tccaaaccgc tcagcacctc ttgttcaaac gcttggtact cgtttgcttc ccgcagcact
    10561 tcatcatcaa agacggcata ctgcatccgt ttgaggcggg cgtattcctt ggcctgaatc
    10621 gcattataca acgctttgtc tgccaactgg tcgccatgtc gcaagaaata gataccataa
    10681 tgcatctgta tcgcgtaccc tttttcgctg gcgggtgaat aatcgataat agcgcgattg
    10741 agttcttcaa gccaggcgat catctctgct tgcgaatgac ttggtttcac gacacaaaag
    10801 gtatattccg aaacccttgc tgccagttca tccgtttgca gactttgccg gatggtcccg
    10861 gcgatatgct tcaagaggga atagacttca tcgatgccgt aaagtctgcc gatccctttc
    10921 atgctgctgt caattatgat aacagcgtac aaagagcggt ttttgtcatt gatcaattgt
    10981 tggtaatggt aatccaaccc gtccaggctg gagagcccgg taaaggtatc ggtattgcga
    11041 ttggttaagt tgagtttctt ttgttcccgg agttttttcg caagatgcat cccccagccc
    11101 agaatgatca gcaacagcag cccagccaga acgataaaag tcatggaaag gccgctcttt
    11161 tttccgctca gataatgctg cagaatggct tccttttctc catcggtcat gttttgcagc
    11221 gactttgcaa agatactgac cagttccggg gcggcagcat aggtaaatag aaaggttagg
    11281 tcaatcggct tgccactgta atcaacggaa aaattcaccc ggtcagagac ggtgttcgtt
    11341 ggcagggagg tcagtgccag catgacttcc acctgtccgt tttgggccaa ttcggcagca
    11401 ttggcctggt ccgtattaat atactgaaac accagcccgg agtcggccgc tacctgccgg
    11461 taaaggtcag gtacaatccc ggcgtattca cgtagctgcc ggtcatagta ttccagcgga
    11521 taaaaatcag gatgacctgc cacatataca atcttatggc tggcaacata gtctttttcc
    11581 tgcttgtcaa acaaagcaaa aggaatgaaa tgaaagatca acatctcgac cagcaagaat
    11641 gttgtaaaag cgattttcat tttaccctct cccctcccct gggtgtttat ctcaggttga
    11701 ttttttgccg ttcaattttg ccccagtctg tttttcgtct gttgtagcca ataaaggcgg
    11761 taatccgtac ccaggccata atgaaacgca ggacagatat ttcaattaca cacaaaccgg
    11821 tggccttgat gatatctgtc aagtatagtg tctgattctg cgtataaatg cggttaataa
    11881 aagctgtcgt ggtcaaaatc acgccaaaga tgacatagat caggaaaaac atgatcataa
    11941 acttcacatt gatcaattcc accagaaaag caagcacaat cgttataatc ccaaacaatt
    12001 cgataaatgg tgaagccagc tcataaacca ggaaataaaa ataggatatc caactgattg
    12061 ctccaaatcg gggattggca aatacctcct tgtatttcca catactctgg aagagtccca
    12121 aatgccagcg tcttctttgc gaggtcaaat catgcagccg ttccggaacc tgcgtccagc
    12181 agatcgcatc cggcgcatat ttgatcagat aaggacgatt gttggcccgg cagaatacat
    12241 gcagtttaac caccagttcc atatcctcac cgatggtgct gtgatcataa ccgccggcat
    12301 caatcacgat gtctttttta aacatcccga aagcacctga gataatcaga ttcccgttaa
    12361 actgatcaag caacaagcgc gatgccagga aagatctttc atattccagc gtttgcatgg
    12421 ccaccagtat tttgggcggc aagcggtaat tgatcacccg gccctccatt attttcaggc
    12481 cattggaggg tctgaccagt ccccccgcag caataatgtt gttgtcttcg agaatggtat
    12541 tcataatctc tttgagcgaa tcctgctgta acaccgaatc cgcatccata cagataaaat
    12601 aaggaaactt ggaagcatta attcccatat tcagagcatc cgccttgccg ccgttttcct
    12661 tttttatcag ggtgatgggt accttttgct ggtcggtgct gtagatcgct cggaccggct
    12721 tgcatttaac agcccgattt atcggctggc ggatctgttt catatcgaaa tgttcaatca
    12781 ttttttggct ggtactgtcg ctggaaccat cgtccaccac cacgatttcg tacaattggt
    12841 aatccaggct gagcagtgat ttgaccgaat caactaccgt gacctcttcg ttgtaagccg
    12901 gaacaataat ggtaatcggc atataataat cgtggttcag cacattcttt agccgttctt
    12961 ttttccgttt atcaaacaaa acagtgccgc caacgatgac agacaagaac aaaaaggtgg
    13021 aataccctat cagataagcg ataaaaaata ttccagtata ttctattaca atcctaatga
    13081 aatccatcgt atcacgttcc tatcccatac tgggtttgaa attggggttg tactccataa
    13141 agtactgcag gatctcccgg gcaaagcggt catcgccatt gattacctga gcgatctcag
    13201 ccaggtccat ctgcatggaa gtcaaacttt tggccgcatt gtaacggata taccagttac
    13261 tgctggacag agccttttgc agtgcacctg ccacttcctg atcagggtag gcggccaatg
    13321 ccgtggctgc cagcgccgga tattgccagc ggtctttgtc cttgatctgc agaaaggcca
    13381 gcaatttttc ccgagccgcc gggaaggggt acctggtgaa atagcgaatg attgcgaatt
    13441 ggacctccgg atgaacactc gcatccccca tcaacaccgc cagttcttcc tggtaggcac
    13501 ccgtagtaaa gcggagatag tcgagcaaag ccacctgaat ggggatacga aattggaaaa
    13561 gattcttcca gagagtctgg ctcaattcct gatgactgcc ttgaaaggtc agcagtccgt
    13621 ccgtcagcaa tttaccgtga aaatagattt ccagccggtc caggattcgg atcccccgca
    13681 tgacgctgga gacgtctcca aagctgaaaa gagctttcat tacattggcc tgacagtgga
    13741 ttgagtcctc ctgaagatag aaaaaaagaa ttttggccag cgcatcagta ttcaagtttc
    13801 gggccagccg tggatgggct agcacatggg caaaataact tttactgatg accttttgtt
    13861 gttggtattt cctcgccaga aactcgaata ttggactcaa cgtacgcaga tactcgtcaa
    13921 aataatcttg ttgcccgact gctttgcggt ccagcagttc ggcaaaagcc atcaggtatt
    13981 tgacccgggg caattctttt tgtaaatatt gctggtgttg cctggtaacc ggcaaccctt
    14041 tttccaggcg ctccagttgc ttgtcgagca agcgattcag cttttggctt cgttttatat
    14101 actgtcgctg gtcaatcacc gtccacagaa tccaaaaaat attaaaaagg attaggctga
    14161 agcataacca tatatacata agaatcattt tttctacaaa catttcctgt cccctttatt
    14221 gccagcgctg ctgcacgata taataggctg gcttgagtcg ttcgtggtaa cttgcctcgc
    14281 ctgcccatcc tttgagtttc accctgccca tggctatccg ctgcgaacct gtgacagcat
    14341 cgccaacgcc gatatacatc gctttgatcg gcatgtattc caccccgtag tgcgcataat
    14401 aatcagcgtg aatcatcatt gcggatggat cggagaaatt cagcaactgc cagggaatgc
    14461 gcatttcaat caagtcatcc ccatagaaaa aatcagccag cgaattgtaa ctgctgcttt
    14521 tcgggttggc gttgccgtgt gtcagcgctc cggtctcaat ggtctggtag agattgtgga
    14581 taaagcgttc ctggtcactc aattggataa tgtccacacc aggatcatat tgtttcttta
    14641 aaaccattct tatcttgtca aaaaccggga gagccttatc gggaacaaat tcataaggct
    14701 ccagccccgt ggtggatttt tcatacatcg cccgcatgac atcataacgc cgatgcacca
    14761 acactctgct gttgttctgc ccgtttatca gcatcaaaaa gtccgctggt ctctgaaaac
    14821 gcaccttata ctgggcacta tagttgctgc cgctttgagg ggttgtgtct atcggcaggt
    14881 acagcatttc atcttgccgc aggtcttttt tcctgaccaa tagataaata tattgctcat
    14941 cgtacttcag ggatagcgat agcgtcggtt ccgcgataat gatgtcatct tcattccatt
    15001 ctccaggctc tccgtcgaca tagcagacac tctcctgcgc gcccgcatca aagctcagca
    15061 aaccgtaaaa ttgaaccagg ctctgagcgt catgccagtt tttaacctga tttaggtcta
    15121 cggcatgctg ggtattccag gcagcatgac tccagtcatc ctgccaggca gagatgaccg
    15181 ccccgcagca tcctgcctga acgatctgct catacgtatc tgccaggaga actccctgcg
    15241 cttgctcggt cagtctttcc tccaccttgc tttccgcgcc ccgcgcagtc gaaaaaccat
    15301 agctggtgac caagaccgga taatcataat acagattcag catgttaata tacccgtcat
    15361 aaatggtatt gacgtctact tgggacagat actctgccag ccggagtctt tcgctgtccg
    15421 ccagacactc tgtaaaatag cgatagtagg aatacatgcg gtaggaaacc acaaatccag
    15481 acgccaccgc gtcggtcagt tggatatgat tggcatccac ctgggcgact ttatcaatct
    15541 gaatattgac attatacaaa tattcaaagg ggtcggccgt cggttcgtta atgaaggaaa
    15601 ccagtctttg ttgtttgtat ttcccggctt cataggacag aatctcgttc atgacttccg
    15661 ccagcatatt ttcaaaagcg ctcgcctccg ccgtggcaaa gacatatttc ccttgataat
    15721 ggcgttccgg ttcctgatga tggtcggtat aggcaatggt atagggaaac caggtagttc
    15781 ccacgataaa gcccaacgtc cagggagata cgtcctttcg gtaattgccg atgcccgctc
    15841 ccttgttcag cgtgatcagc ctttggccgt gaatgacatc cacggcgata cggcagttgg
    15901 ttatcagatt ttcgaaaaat gcttcgctgt aggcgccgtt ggctgcgttt tgttcataat
    15961 cgcggacagc aatgctctgc agcaagtaaa ggggctcggc cgccttttgg ttatagtcgt
    16021 aaaaagcatt gtaaaaggcc tcatccataa tgtgattaac tttgacggta ttggccccca
    16081 tctcgccaat cagctgcagc catctcagat aggttggttc atcaacttcg tagtcattgg
    16141 caaaacagcc gggcttgacg ctttccagtt ccacgccctt gactaccagt tgctcgagtc
    16201 ggtttccctg cgacagataa atcctggctc gatctgtttt cgccatcgca ttgacgggct
    16261 gctcgggatg aaaatcataa taaaggccca aagaaagagt caggtattga tagataaaat
    16321 agatcatgag aaggccaaaa catacggcaa tatacttttt catcttttcc gctcacctgg
    16381 cttccttatg gcaatggcag aaagtgcttg atttttgatc cgtgacaatt ctgctgcagg
    16441 atatcgatat gtttatccat ccattcgccc cgcttgacga tgatggtttt taattggtct
    16501 atggcttctt cgtaactggt gggatttaat tcaatcggcc ggcggctgac ccccggtttt
    16561 tgatgttcgc gttcaaacgt atagccccac accatatagt tgcgatcgat ggctccgccc
    16621 aggtagtttg tggtttcatc aatgtagttc agcaggtatt catcactgag atagctcttg
    16681 cgcaacgctt tgtagcgggc aatcactttg ttgacaaaat actcatcccg caacagcatc
    16741 tgataccagg ttcgatcaat aaattgaagg tccgtcccgt caaaatcaag aggaataaaa
    16801 ttgttgaagg cattattgta atcccatacc ggtcccatat gcagcttgcc gcgaatatcc
    16861 ttgtaaagaa aagtggaacg cgaacacatg tcgttgttca ggaagaactc ctgcagcaca
    16921 tagtaatcaa caaaggaatc cacatcgata tacgcccgat acccccgcgc cgggtctttg
    16981 aagtcaaaag aataaagggc tttttcaaat tgactgatat cgtcttcgat aaaagtgcgc
    17041 gtctccggcg tcatcagcaa acgaggcgga taaacaatgg taaatccagt attgtattta
    17101 ttgttcagcg tataataggt gaaattatcc agggaacgtt cgttgtgctc atactcatcc
    17161 attcttacaa tataactgga ggctacatga tccggatcat agccggtgag attgacccgg
    17221 ttttcacttc gggcgaccgt ctccataaac aaatacacgc cctggtagcg cccgtcgaga
    17281 aaacattcgc agaacctgac attgggagcg tagcccatca cttcggccga tatattcatg
    17341 cccaagtaat tgcggagcag cgttttgtcc agaaatggac catacagcgc ccattcatca
    17401 tgggcaggca ttcccatgac agctagttcc cggtcatagc catgtgtatc gatgaaacgg
    17461 atcgcataac tttttttatc aaagatgcgg gaggaattgc cgcggattct cagccaggcg
    17521 gaagcatcca ctgccttggt atcgccgatg tggttgtttg cccgggcatt gtcaatgaca
    17581 ctgatttcgg caatgatcat ggtctcaccg ttgtcacctg tttccagcgc gatggtattg
    17641 ccgagctcat tcaggattgg ttcgccgggt atcttctgtc cgccggtcga gatattgaca
    17701 atcggcaggt gcgtacaaaa cacttcattc tggtgtacgc acttctgaac ggtatcttcc
    17761 cggtgctgat gaacccggcg ctgatcactc ttcggcatta ccgtaatgaa agtcgtggtc
    17821 agaatcatga tcatcacagc agccatcagt atgattttgg gtttcataga acatccctca
    17881 tcaactgctt atttcatcat tttgcgttac cagattgacg tactccacac tgcctaattc
    17941 atagagaatc tcggtaatat tcttttcccg ggtgatggtt ttatcaacga cttttttggt
    18001 cagttcgtag atgaattcca cgttggcttc ggtggtgttt tttacacgca gctgggcttt
    18061 atcatcaaaa tagtcgaata taatggcttc gattttacgg tcaatgttgc gcgctgcccg
    18121 gataatcagc agaattcggt tgtcattgcg gatttttccg aaaaccagca gaataataaa
    18181 taccgcacaa ctgcccaggg ctgcaaccag ataatgacct accccgcagc agatgcccac
    18241 aataatcgcc cagaatatat agacggtatc ccgggaatcc ttgatgacgg tccggaagcg
    18301 gacaatggac aaggccccca ccatgcccag tgacaaggca atattattgc cgataaccgt
    18361 catcaccgta gtcgtcagaa tggttagaat taccagcgat atattaaact tttcactgta
    18421 aatcgtgccg gaatgggaca aacgataaga taaaaagata acgatagcaa tcaaaatggc
    18481 aaccacaata tgtaatacga tactttccca accaagctcg ctgctttgct gaaagatttg
    18541 cagcaaatct tgtttcatcc ccaatccccc cgttaaaaaa tatagtttaa ggccacggaa
    18601 cgtcccagac agtatttgct gaccgccagt tcactgcgat taatccgatt gataaattca
    18661 cggatatagc tcaggaaaaa cccgttgtat ttaatctcca agacgacatt gaacggattg
    18721 aacacgggat acatggtgag attctccgca aaaatgtcaa aacaggattc ggtggcggta
    18781 atgttctggt caaatgttac ccgtatcttg ttttcctggg cgataaaagc tttgcgattg
    18841 tactgcacga tggttttggg acgataacag tacatatgca taaagccgaa acactcggcc
    18901 gcaaaaggat cgctatacgt caacaagggc gaataattgc cccggcacat ctccgcagca
    18961 tcagcccggt tcatgcgcaa ggaacgtttc aattgctggg taccgtgctt ttgcttcatt
    19021 tccagcaccg caaaatcact gcggggatcg tagttgcgca gtctgacctt gcgacgtact
    19081 tccaccccgt ctagtttctc aaaataatcc cggtctgtca gcgtatcgaa atacagagaa
    19141 cgaatcatat agccatgggt cccgttgtga ctgtcctgca tcatgaagtt ctccagatag
    19201 cgactgtgtt tgatggcatc ctcgatggta atcaggaatt tcttttcctg tcgcattacc
    19261 gtattcactc caggttcctc cttgtcatgc tgatcatgat ggaacggatg gcgcaacaac
    19321 tgatgatatc actctattgt attccctgaa ctatttttta tcatgggttt ttttatgagc
    19381 gcttttatgt ctgatccttc ttctttgatg ggtttctttc cagcattcgc tgcaatactt
    19441 ggtcagctga aaggatttgt cggtgatttc ttttccgcac ttttcacaat gcctgcgttt
    19501 actctcctgt aaacacgcat cacaatatct ggtcagttga aaggatttat cggtgatctc
    19561 ttttccgcaa gtgatgcagt gccgggtgtg gagtgcgcgg taacacgcat cgcaatagag
    19621 cgttagttga aaggatttat cggcaatctc cctgccgcat tttatgcaat tgctcatatc
    19681 atagccctcc ctttacaagg gggaatttgc atttcaagcc caattttaaa tatataattc
    19741 tgcaacaatc gacgcaattc ctttttttca cagatgaatc tgccccaata ttatcagaat
    19801 attgtcactc atattatata taggcataca ttactaataa atctgtcaaa aaacaaggag
    19861 gaaatacttt gtcatttgaa atcgtttatc atctgcgtcg agctttggaa aaattaccac
    19921 agctacatta cgggcgcaaa ctcagttata ttccctcggc ctatgacccc cgcgattatc
    19981 gttatgcgaa aatgatcgtt gctgcccaac cgggtgcgac cttacccatc gattaccgtg
    20041 accagcttcc tcccgtattt gaccaggaag accggggcac ctgtgtggcc tgtgcagcag
    20101 cctggacggt gaaagcctat gaagaaatta aacaggcgga ttatccttca gaaggcctct
    20161 cggcatcatt cctgtactct atgtgcaagg aaaatgacgg tcttccctgt ctggaaggca
    20221 ctcagcccaa ggtcgccatg caagttttga gaaaatacgg catctgtcca gaagcaatca
    20281 tgccctatgc ccagctgagc gaactgccct gccccaaagt cccggggatt cctcaaaagg
    20341 ctatggaaac agccagcccc tataaaatac agacttatgc gcagctttgt tccacttctg
    20401 attcagaccg tcacgaactg cttgaagtca tccgccaggc tttgcaaaat gaaggtccgt
    20461 ttatgctagc cttgctggtc tgtgaaaact tccaaccaga cgatgattac gaattgcctt
    20521 taccggccgg actcttgcgg ggtggtcacg cagtggggat tgtgggtgat ctgcctgagc
    20581 gggaatgtct gatcctgcgc aattcctggg gcagcagttg gggtgaagac ggctatgcct
    20641 acctccccta ttcctggttg aatcacaaaa tctgcggcag ttggtctgtt tccgaagcat
    20701 ggacggcgac cgatatgacc ttgctgccgc aagcccgtcg catcgtggtc acgcccggat
    20761 taaaaaccat caaagtggac ggcaaacgag ccgcaatgaa taacaccgcc tttttcagca
    20821 aatccgcgcc gcatcagaac gcactgcagg aactggttcg caatatgggc tatcgacttg
    20881 attgggaagg caaacaggcg gtgtttaccc ggatgctgtg ataagctgac cgctgcgcag
    20941 atgggttgac ttgcgaccac tggtcaatcc ggagatgata ttcagctcct gcgaatcatg
    21001 gctttttttt catggccggt gcggcctgac gacgcgtatc acggggacga aggatttccg
    21061 ggcggatgtt ttgtaccggt accggtgacc ggaccaggat ggttttgagg gcgcgccagt
    21121 ggaagggaat gagcggccag aggtagggta cctgaaaaga tttggtgaaa gccagcacgg
    21181 tcaggaccag cagtgtccgg gcaagcaagc cggggccacg gaataatccg gtacagatga
    21241 gaataaacag gcgcgccagg cggttggcca ttcccatctc gtaactgggt gtacaaaagg
    21301 tgccaatcgc cgccatggcg gtatacaaaa tcgtttccgg gatcaacaaa ccaatctgca
    21361 ctgccacatc accgatcagc aaggctgcaa tcacccccag tgcagtcgcc attggcgcgg
    21421 gagtatgaat ggcagccata cgcaacagat caagactgat ctccgccagg aaaaactgca
    21481 gaaatatagg cacgttgccg gtctttttgg gtccgataaa atcaagtcca gccggcagca
    21541 gatccggtga tagcgctgcc agcagccaca gcggagttaa aaataccgaa gccagaatcg
    21601 cagcaaaacg aatccagcga atatataccc cgaccgaggg attctgccgg tattcttcgg
    21661 catgctgcaa atgatgaaaa aaagtcgccg gggcgataat cacactgggc gaggtatcca
    21721 ccacgatcag cacatgtccc tccaacaggt gcagggacgc cacatccggc cgttccgtat
    21781 agcgcacttt ggggaaggga ttccatgtat tgccgccgat gatcaactct tcgactgatt
    21841 tttccgccat gggaatgccg tcaattttga tcgccccaat cctttcctta atggtttcca
    21901 ccaggtcagg attggcaatg tcttttatat aaataacagc tacatcggac ttggaacgct
    21961 tgccgacccg cagcagttcc acccgcagct tgggatctcg cacacggcgc cgaatcagcg
    22021 cagtattaaa cacgaccgtt tcagtaaaac cgtcgcggga accgcgcacc acccgttcaa
    22081 tatccggttc ctccggtccc cgggccggat aggtgcgggc atcaataatg aagccctcac
    22141 tgcttccgtc aacaattaaa agaatcgagc cggacaggac cgcatataaa agctcgtcaa
    22201 tcttgccttc tgtcgataat tctatataag gaatcccccg atgcagcagt tgctccatcg
    22261 tattttcaaa atatttttcc ttgcgcaaga caaacaatga ttccatgacc tcggtgagga
    22321 tatcatcctt gaccaacccg tcaaaacaga tcaaagccgc tttcagaccg accactttta
    22381 tttctctgac gatcacatca aagctctcgc cttcccccag ttcctttttg aacaaatcca
    22441 aattgtcatt gtaattgcgg ctcaagctgg catgttgctg acgcttaaac aaaataaaac
    22501 agctccttga acgatgaata aatacgccga ttgcctcttt gtaagatatt gttggccaat
    22561 cactgcacat ctattctttt gaaccgttca aggagcattg cttgagctta tcggtattgc
    22621 actgttcgac tgggcaaaga acattgaacg acgaccagat gattgatggt tgacccggcc
    22681 agtgaacttt caagactttt tttgacttaa acgcccactg gggaataagc caagatcatc
    22741 gtaccaaccc aactgatcct ttgaaacgat acagtcagca acgcctgttt aatcctcaaa
    22801 atatggatcg ctgatttcca ccgtgacccg gtcgtccgaa cgcagccgaa tggccaggct
    22861 atcaattttg gtcagttcat attcaaagaa ataacgcccg gtaaagatct tgccttgata
    22921 gaaagctgga tcaaccgcac cctgctgcag cgctgtctgc gccaccgtca tctgtttcag
    22981 ccactgccag gcgatggcga tctgccccat aaagtcaaga tagagcacac tgtccgccag
    23041 gaaggcttcc ttggaaaggg atgctttttt ggccagcagt tgcgctgtca aatccttcat
    23101 cagttgcact gcctctgcca gcgctttgac acagggtgcc agctgcggat gaagctgcgc
    23161 ctggtcaagg gtcttttcca cttcactgat gtacagcgca aacgccttgc cgccatgcat
    23221 actgaccttg cggcccagca ggtcctgccc ttgaatgccg gtggtacctt cgtggatcgg
    23281 atcaatacgg atatcgcgga agtactgttc caccggaaaa tcgccgcaat atccgtagcc
    23341 gcccaggatt tgaatggcag cgctggtagc cagcacgccc atctcggacg gataactctt
    23401 caggatcggg atcagaaagt ccaccagtag accattttcc tcccggtcga tattcacgtt
    23461 ttcatagtcc tgatacagag aaacctgcag tgccaggcac agcgcccctt ccatgaccga
    23521 acgctggaac agcagcatgc gtttgatgtc ggcatgctca atcagcggca ccatcggcgt
    23581 cagcggatcc ttctggtcgg gattgcgccc ttgtttgcgc tcgcgggcat attccagcga
    23641 agcatagtat gctccggaaa tcttgctgat ggccccgatg ccgatattaa tacgctcctc
    23701 gttcatcatc ttgaacatat gggccagtcc tttgccctct tgtcccacca gccagccgcg
    23761 gcaatccttt tcatcaccca tactgagctg acagatgggc gaaccccgat aacccagttt
    23821 gtgttcgatg ccggcgcaga agacgtcgtt gtattccaat tcccccttgt cattgatgcg
    23881 cttttccggc accacaaaca gtgagatgcc gcccaccccg gctggtgctc ccttgacccg
    23941 ggccagcatc agattgacga cattctccgt cacatcgttc ttggcggcag aaataaagat
    24001 ctttttgcct ttgatcagat agtagccctg atcggttggt tccgctgtag tcgtcagatc
    24061 gcccagcgaa ctccccgcat ctggttcggt cagcgccatg gtcccctgcc actcgccagc
    24121 atacattttc tccagataga gatccttcat ttccggggtg ccgaagctgt agatgaggtt
    24181 ggctgccccg gcgctcagca actgatatac attcatagag taattggccg cgccaaacaa
    24241 gaaataggcc gccgccttga tggttagagg cagctgctgg ccaccgatct catagggttt
    24301 ttccgcctga atccagccgc cttcgcccat ttccttcaga aaatccttga tggccggatt
    24361 cacatgtaca ttgccgtttt cccaataagg cggatcggtg tccatctcct taaaataagg
    24421 aaacatcatg tccgtgctga ttttcatggc cgtgtcgatg atcattttaa acgtttcctg
    24481 gttgtgatcc tgaaaatact caatccggtt cagactggca gcatcaaaaa cctcctccag
    24541 taaaaaggca aggttccgtc tgctggcaaa tttgttggcc atcctgctct cctccttaaa
    24601 atttcttatc tttaaaaaaa tatttactcg cactcattta gaacatttat ccagatgttc
    24661 atccgcaccg aaagcacttg cgaatcctgt caatttcttt cctcttcgtt tattacaaca
    24721 aacgttttat aatcccttcc aaatcaaaag gcggggtgta ttcttcccgg gcgctgctgc
    24781 gctcctgcat ataaccgctg gtcagtccca ggcgaacggc ttccgctact accgtttcat
    24841 attcaaatga agtgatccgg cggtttagct ccgggtgctc gacgcttttg taaaccgggg
    24901 tgtactggct catcagactt aacatgaatt tatccggcgg caggttttca ctgagccatt
    24961 gcagaatggc aaaactgtcc tgtcttgcgc cgggcaggac cagatgcctg atcacaacac
    25021 ccttttgcaa catgccttcc tcatcaaaga ccaatccgcc ggtctgggcg gtcatggctt
    25081 ggatggctgc cgttgcggca gaaaaataat cgcgggcgtg ggaatattgc caggctaatt
    25141 catcgctgta atattttaaa tcgggaagat atacatccac atatcctgcc aaagcctgga
    25201 tgacctcaac ccgttcatag ccgctgctgt tgtatacaac cgggatgtgc aactgttcct
    25261 tgatcaggtc gagagccggc aaaatctggt ccagatactg tgtggcggtg accagattga
    25321 ggttatgcgc gccctgtgtc tgcagttcca tacagatttc ggccaatcgt tccaccgaaa
    25381 tctcttgtcc aaagccggcg ctgctgatct gatggttctg gcaatagcaa caccgcaacg
    25441 tacaaccgct gaaaaagatg gttccgctgc cgcgggtacc gctgatacac ggttcttccc
    25501 agaagtgcag ggctgcccgg gcagctttga tctggctgct gctctggcaa aaaccggtct
    25561 gactggaacg gtcgatcccg cacatccgcg gacataaatt gcaggggttg gtggtcaggg
    25621 aaaatgcgtt tttatagttt gtcccacgca ttgatttttt ctccaaacgc tttcattctg
    25681 cgcgcattga gatcgcccta agcggctttc tttaagctcg ctggcattgc caaacatcgt
    25741 tgccaaactt gacatctcta ttatccttaa atctctgcag catcgcttgt atttcattgg
    25801 gattcagaac gcatttcatg taattcatcc tttcgttgga agtttttcgt tatccttatt
    25861 gtaacccctg taatgacgat tccaacagtt attttgtact gtttatcgaa aaatcgaacg
    25921 gcaaactaat atggatactt ctctgccaag agaagaaatt tccccctacc gtatgaaaag
    25981 ccgccagaaa ataaatattt ttcctggcgg ttacctttct caagcaatgt tttacttcaa
    26041 atataatcgt tccagttttc gcagggtatc aatggtctgc agtttcacca tattggattc
    26101 tttttcccat gctgccaatt ctgttaagaa ggagatggaa ctgctgtctc cgacataata
    26161 gagggcttcg gtaatggaaa gccgcaccgt cgcacagcag tcgaacaagg acagatgtaa
    26221 ctcttccagc acttcagtcg tgaagtgttc tctccgatct ctggcaatat cgcgaatttg
    26281 gtcggcggct ttgatcctga tatatcgtcc gcccgacatg ggatgttcgg ggtattgctg
    26341 ttgaaacctg taatcgtatc cccagggccc cttgcgaatg attaattcat catgcctgcc
    26401 cgctctttcc aattcggcca tggtcataaa atttgttttc agcgctttga gcagttcacc
    26461 cagattggga gaattatttt gcatggctgt acctccatac gcatttttat aatcgatttt
    26521 cctgcctatc tcagattcaa tttacagcgt tatagtgaca ctattttgtc atattaaatc
    26581 atggttattg gagaatttag cgagttaaac gctcttattt cgacattgaa tatatcattt
    26641 cagctaataa tggtataatg aattatctaa taattcggta ttcggtaccg acagacggga
    26701 ggagagatcg tgcaagaaca gtcaaagaaa actacttcgc cagcagaaga acaggttttt
    26761 ggttgcatgt gtgttggcaa ctgtcagtcc agatgtcgtc tcttcgtgca tgtgcgcgat
    26821 ggcaaagtgg ttaaaacttc gatggccccc ttccccgatc cccgctacaa tcgtgtctgc
    26881 ttgcggggac tcagtcacgt tcagcaaatc tacaatccca atcgcatcaa gtatccgatg
    26941 aagcgtgttg gtgaacgggg cgaggacaaa tgggaacgca tctcctggga agaagcgatt
    27001 gccaccatcg ctgaaaaatg gaagacctat caggcagaat acggtccgca gtcggttgct
    27061 ttttccacca tcagcggtga catggccatg atcaatgggc tatacagcgc ccagcgtatt
    27121 gctcaaacca tgggagccac gttaaccgaa tattcactcg acatggcgga cacttacgga
    27181 ttgggcaggg tcattggcaa catgggtacc tttaaccaga gcaatgaccc ggctgatctg
    27241 gccaatgcca aaaccgttct ggtatgggcg gccaacccga ccgaatccca tcctcactcc
    27301 tggcggtata taatgcaagc gcaggaagcc ggcgccaaag tagttgtcat tgacccccgt
    27361 tatacaacca ccgccgccaa agctgacagg tatatcccca tccgtcctgg atctgatgct
    27421 gttatggccc tggctatggc ggaaacgatt attgccgagc agctatatga cgctgaatac
    27481 ctgcgttatc atacggtggc gccgtttttg gtgcgggaag acactaaacg cttcctgcgg
    27541 cagagccacc tgaccgggat tgccccggaa gccggacagc ctgatccgta catcgtctgg
    27601 gacaaggaag cccatgcagg caaggttttg gcggaagttc ccgacccgca gctggaaggc
    27661 acctatacca ttgacggtat caaagtgagt acagctttta cggttttaaa ggaattttta
    27721 acagactaca cccctgagaa agctactggt ttgtgtgatg ttccgacgga aaccatccgt
    27781 gagactgccc gtctgtatgc caccaatacc ccggccagta tctttaacgg ctttattcaa
    27841 tatgacaatg ggttacagac gggtcatgcc tggggcatac tggtctccct gaccggcaat
    27901 attgcccgga atggttcttc cgtaggccat tgcggcatgg ctttttacag ttgcaatttc
    27961 attaaatacc tgttcccccg cggcaaaaac tcccccacca ttccctggct gctgttgccg
    28021 gaggtcttaa aaaccgggga gttttatggc aagccccatc cgataaaatc catgtacttc
    28081 ctttgcagca atgcagtcac caacaatacc aatcaaaacc ggatcatcaa tgagatcttc
    28141 cctgcgatcg attttatcgt ggtggccgac attgtccata ccgacacgac caaatatgcc
    28201 gacctgatcc tgccggtggc gcattggttc gaaatgctgg aatttgtcca aggcccggat
    28261 catccttatt cagtcctttc cgaaaaagct ctggaaccgg ctttcgaatc aaaatcaaat
    28321 acggatattt ttagaatgct gagcgcggcg ttgggctttg gagaggttta caactatacg
    28381 gatgaagagt ttattcaaat gttgattgat acggatttat cccgtcagct aggtctcact
    28441 tgggaaagac tgaaggagga aaaggtcatt caaacactgc ccagttgctg ggtcaactgg
    28501 gccggcaata aattccccac cccctccgga cgactggaat tttatctgga agatccccag
    28561 ccacgccttg attgcggcat tcccatcgat caggacaagg aacgtttgcc ggtctttacc
    28621 ccgccgattg aggcctggcc ggacaacccc ctgaagcaga agtacccttt aatctaccac
    28681 acggaaaggg ttcgctggcg catgcacagt caatggtcaa gcaccccctg gctgcgtgag
    28741 cttgacccgg agcccacggt gaaaatcaac ccctcggatg cggacagccg cggcatcaaa
    28801 accggcgata tggtggaaat cttcaatgac cgcgggcatg tggtgttaaa ggctattctt
    28861 tccgagggcg aacccccagg catggtgagt gttcccaagg gctggcaccg tgatcaattc
    28921 aaggctggtt cctaccagga actgaccacg gatcatgtca actattcgag ttacaatcaa
    28981 tctttttacg acgtactgtg cgaagtacgc aaagtatagg aaaggggggt cacctgcatg
    29041 ggaaataaac gcctggtcat gctgatcgat atcaatcgct gcattggctg ccatgcctgt
    29101 gccgttgcct gtaaagcaaa caataatctg cccaatgaaa tttggtggaa tcaagtcctc
    29161 acccacggcg gcaagagcat ggacacgcct cggggacaat atcccaacct gcaattgcag
    29221 tttcttcccc tggcctgcca gcactgtgac aacccgccct gcgtcaaggc atgtcccacc
    29281 ggggcaacct atcaggccga caacggaatc gttatgatgg attacgacaa atgtgtgggg
    29341 tgcggttcct gcatcctcgc ttgcccatac aaatatgtac ggcagtttaa cgaaaaagaa
    29401 ccacaataca tgcttgactt ccccaccggc aatgaaggag tgccggcaca gcaaaaaggc
    29461 actgtttcca aatgtacatt ttgtcatacc ctggtggcaa agggtgaaac accggcctgt
    29521 attgaggtgt gtccggtgaa tgcccgttac tttggagacc tgaccgacaa ggagagtacc
    29581 gtgttcaagg ttttgcagga aaaccagcat ctgcagctgc tgaccgaacg tggtaccaat
    29641 ccatcggtct attatctgac cggccgtaaa ccccaggacg ggcataaaca atgaaaccgg
    29701 aggtgattga tcatgttgcg taaagaatgg ccgttaataa ccaataccat cctggcgcag
    29761 ttggctgccg gactgtttat atttttggca tgggaccgca tcgttttgtc ccgggaatca
    29821 ttgcaactgg cgttaacatc gactgcccaa ggcctggcct ggacgggtcc ggtcattgtc
    29881 ctggcaatgt tgctgtcctt gttccacctg ggcaaacctt tccgtgctta tcgggccatg
    29941 gccaacctgc cggtctcctg gctgagctgg gaaatctttt tttccggtgc cttcctgttg
    30001 ctgtggatta tttttatggt gtttgaaagc agcgattcca tcgctctgcc tctaatggtt
    30061 gcggctgggc tggtgggcct tttgaatgtc atcagcatga gcaatattta ctcttccacc
    30121 ggacggccgg gctggaaagg taccggcacg tatcttgatt tcctgggcag cacgcttgtt
    30181 ttcggcagcg tgggcgcggc ctttttctta tc
//
LOCUS       DAOTHZ010000021        29464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1787_length_29464_cov_6.916624, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000021 DAOTHZ010000000
VERSION     DAOTHZ010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 29464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29464
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1787_length_29464_cov_6.916624"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..1503
                     /locus_tag="P5549_04125"
     CDS             352..1503
                     /locus_tag="P5549_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRW12289.1"
                     /translation="MPETIGASARIAVIAIFLLLWGGLNYYLGWRGWHYFGDYINPYR
                     SLYWVLLIFLASTYFLGRWGNHHYPGAVSDALIWLGSYWMAFLFYALLLNVLIDLLKV
                     LDRVWRFMPDCLHHAAGPIAAALFLLIILGLVYGTVNAHRPVVTRYHIHVEKPAGERE
                     KLHIVMVSDIHLGNIVGRDRLEGMVKQINQMQPEIVLLAGDIIDGDIRPFEQQAMGEL
                     LRGIHAPLGVYAVPGNHEYLGGQYRQLLAALQDSGVVVLRDQVLLLDGFYLAGRDDKI
                     SRQRKPLAEVLAGVDHAYPIILLDHNPVDIQESLSNRVDLHLSGHTHAGQMFPLKFFT
                     ARIFEQDWGYLQNGALHVIVSNGYGTWGPPIRIGNRPEIVEILITFQGR"
     gene            1651..12777
                     /locus_tag="P5549_04130"
     CDS             1651..12777
                     /locus_tag="P5549_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRW12290.1"
                     /translation="MFSRFNIHPHLIRKRRRIGLFLTALLLLQVLTPALLASPVQSAE
                     EMQPLPGRIALFHASAVPALVSPGRLSTPEENANQLNNITTQKDALSSFAYINDNAEI
                     LVAEGNQQDITLPPKTPQPEVGMATKPAVAAREIIVCYENDESFGMRPQSLEEENGTP
                     KENTRLSVVALSAEDDPVSVIEQLEEDPAVKYAEPNYILQAQVAPNDQSYVDQWALSE
                     INAISAWDTAVEAEGALAPVTVAVIDSGVDGNHEDLAGKILPGYNTVGGVNSADSSDD
                     SGNGHGTHIAGVIAAQTNNVTGVAGASGNLPVDVLPVKVLDKAGYGTMYDISMGIRYA
                     VDWAEMNNRKIVINLSLGARLPDYPMTLAAAVQYAQDKGALVVAAAGNDKAGLESFYP
                     ACLPGVLAVAASGKDHHMAGFSNYGDIPAPGVDILSTLPGNRYGRLSGTSQAAAFVSA
                     TAAMQWSAYPDKSAAQAQEALISGRSDYPEGCKVYYWGTVCSYHYYVLNANQAVYNLG
                     EEQNIYDTIEFANTAYDAHLSGEIPYIAKVSNPAKVAEVQFWLNPYGESIHLGTVTGP
                     LASGIYTYQWDSTALLNGKPLPDGEYTVEARSMDSSGVQLAACSYDFKLANESSSGLH
                     LAVKKPDASLAAGALVGVYHRYFNEDYGQIVYECVWEDEADLQGQINIPNAMAKDGND
                     YLVTALGTEPNFFYYETLRAPGTYILDGTASQAVGLKGLKTDGTALGNAIIMADLLEA
                     SFADANPSPGVAYELQEKKVAVLNAAGEATLNLSPGRYNFRLLDEESTYYLVQHDVLI
                     DTTTTTIDFTPQSGDVATVKLASDSDYYSSTLLLKDSGDDYYGFKEINSDTCLTVTPG
                     SYNAVIKAIKRDAENNIEWNWGLESPNMDLAAGDKKVLQFGTPLSGSLSQWQDEMPVA
                     GESYWMNPAFNDAFGNTTTGIAYQSYTPASLPASAQSSGSVIARMVADKPAEKTDPSG
                     STPQEEQIYVYERSRGDFVLSTQAVSYIRPAIRIYDPQGAQVMPQTSYEQTCSYSFAE
                     WYIPAQQSPGQYSAQVYLDAGTLTNGTCESPPFLFTVQAEASSNIEPSVNVTLLDRMG
                     EPMTGASVDLMQLHDGQYRILDSSTADEAGQASFYSEIAKTGSYALKISGMSADPLVI
                     DSLSEPLALLVPLTAGTVPLNIMVDASRQVLHRISLAAQDQTLQPITSDSLYYYGYRL
                     DEQGNESLVFKTPYEGENVAVWLAPGEYRFEASVNNQYSIAQSLGTSAPATQDLKAQG
                     YLDPLYLLTREVQVSASGEIVLGGSGLGKLDLLLTELGDSYDYYLGGAAFYRQGQTET
                     TLFKPFKDQSIYLTPGAYQAEAVLIRNHYDGQWDYWLGQQLDIQAGSESIWEMNDNFT
                     TQINLADDTYALNQQLTSSHLIKDDKDNRLLAMGLNFEFYNTYLPSSMSERSTAALTV
                     QNQPLEKMAPFFIIKDPQGQEVYRYRDADPNFNALSYWCWNEDGRITALKEQGTFYTG
                     KYQIPENATGGTYMAALELGVGPQGIITAQTPFSVAAVPSAPTLEAFEAPTRAEKISL
                     SGNALPAATVYLQYRFNQGEVVALGNVNADAEGKFTFADISLADEGSYEFSAYASLGG
                     LTGEVCSPQVLVVDRTAPGSPLEPVAVGQDFSHILISWTAPSDTDIDHYLVYREDTLL
                     GTVAANALRQFLDTGLSSEIDYLYEIIAVDQAGNQSEATAVNGRTASSGDQMKPSAPG
                     ELRLNDAPTSANVTLTWTAATDNIAVVAYKVWRSMDGAEPVLLATTEQLSFSDDNLLA
                     ETTFVYTVSALDEAGNESSPSNPLMVTTPALQFTSISYSVPRNMNGLIKEEASITVNL
                     AAQYKRKATAELIYTNWYDETGTLLNEPQERTLIMELPIFQEYTSLGTASYRGSFQFP
                     AGTEQLLSVKATLRDEASHSVTATADALPLFFTGRLELIMAEEALTSGLLDDATAEIW
                     SETKKSGTKTSLSAEGVFTLRNLTPADDYSLRITNQKGQLLKEQSGLHVIAGQNAQIP
                     MTTRLPASLDVQVIDAQSGEPVPGIAVYFNHADESPQGMGLTDEAGWVKNNQGQRPAI
                     PIALSETILQGRVQFTPAQQAVYSHAERLRTTLEPGLNQAMIQLYKRPVGILQGKVTD
                     DAGKDLAEATITAVQYVEERAVSKTVLTDANGNYSLELLSGINSEISFRHRRTSPVED
                     QEVFINDNTVLNMVLPAQAAVTLNVFTKTAENAEPVPLMLDWRVAVHFRINIQNLRTR
                     SVASCYSTWPNVILPDGQAGDQIKITVDGYEGGYSKQEQIVTLDAYRYAEADITLTQQ
                     GKLRASIYDTDGSKQYGERLNVMLYRYDENVWKGAGSLYEYGGGEKTFGPIPEGNYRG
                     VLYWGPRLPVVQNGRDEFAIEGSKIIEDIVIRNGETTELGNIVLPFILPWHSQSGYFK
                     GQEGNSFSASMREAAPNSVVTLRASYRYSGSQSNQPQDMKLSLSIPRGATLVPDSIQV
                     RAEQGAASVPVIEDTQISMNLIPEAETNSSVSGVVSYQVRLSAYPDWPTAGARAWMEF
                     SAGNRTYNEDLGNLEIATPYVSVEAPSPVISRNIKITGQALADQHVRVYDGEYFLGEA
                     TANRYGLWQMQVLLPDRGQPAMHFLNAQTGEGESLIGSLPVRVVFDPNHPTIIRFTMQ
                     QTDGRLVEIDPQLGVASFPYVFVPGMNFTFNITFNNHQAVEEVALIGSNERSDRTYPA
                     VWNEAEQCYSVTAKFEYYSGTPGDLYVSYKIKDTPYQPVIIPSSEEEIRALDEELRRA
                     MPQPWRDATVTVDDPGQVTEPGSGPNPSPQINIALNDNPQQLLKITFGYNEISDFAAN
                     PAIPMIGISNGSSVSNLQDVITELPNGGFRLRRSCVVPASVLNDYLAAGSQSFSAQAS
                     NSVELWVMAESPYEGGMQLADLYDTVKSGFDFKDKLDELSAFQDQAARSCNSGAAGYY
                     NDMAEHLADTAMINLSIKYTLQVGGIALGLTGVGLIGTAAVGVISNLLTEVGEAKWEQ
                     NFNDLKQQLSNDDECKDDEQDDDNDDRDDSDDRDDRDDRDYPKKKKKIAKPKYIWDPS
                     GYVYEAVVDNRIEGVTTTIFEKDTDSGQLVFWDATEYLQENPLVTDSQGRYAWDVPAG
                     YWQVAYEKEGYQTAYSSLLEVPPPQLDVNVPIVSLAPPVVTGAAAAGGGSCLEISFDK
                     YMRLNSMTDTTIAVLLPGQVDEFGNPERITGTISAINPSDDPGAAGAQLAKTIRFTPA
                     QALTVGEAVNLQVSQMVQSYAGRTMAADYSTTLTVPAVTVPEEPAVPGNGSSSGGRSN
                     PPVVDQLNSISQTVSVTSGVQLSSKDDAAVVTIPAGAVSADGILKVSLASKLPFLGSL
                     TAGSQGYDIVLENTTLGGRATISISYDPERFRNIAPEQIGIYYWDESLSKWVYLGGIV
                     DKDNHRVTVTVNHLSLYAVLADTSIPVFKDIHTHWAYAEIVRLAGSKITGGYPDGTFR
                     PDANISRAEFAKLLVEAMGWQAGEGIADFADAADLPVWASGYIATAAAKGVIKGYADS
                     TFRADQLINRAEMATMVIRALSVGQNNQTETIGSNFTDHAAIPSWAQSAVELAVKAGI
                     LKGLPGNVFGPLQNATRAETAVILTRMLDNPKLATVVGQTQTINASGVVAISNISGPH
                     YELRSCSSSQTAKYVLIPNSEVLAERLKTLLGQTVAVAGSLSEGADIYMRGPMLKVTE
                     IKVISNPGGG"
     gene            12965..15415
                     /locus_tag="P5549_04135"
     CDS             12965..15415
                     /locus_tag="P5549_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW12291.1"
                     /translation="MNNDRLKVKAKDLRKNCNPSIFKFQTTAEIKPLKGIIGQERAVR
                     SLDFGLDIKNEGYNIYLSGAFGTGKTTLAKEMLEKKARRKKVPPDWCYVHNFKQHDSP
                     RALQLPAGMGRAFKQDVAEAMQEVMQQAIKALESQDHNTRKSVIMNQFMEETNRMYLQ
                     LEEESRNYGFSISRTANGVSSVPLKNGEILSQEDYVSMSEEEKMDLLKRSAVMQERIN
                     QSIRVYKELEKAVKLNLKDLETETTLKASEPFFEKLLKKYGSYPDVSDYLQDMHQDVL
                     KNLDLFIAEEDTSGMGFFRNMDKKSALRRYQVNHFVDCSDLQYAPVIFESNPTYSNLF
                     GQIEFESEFGVLSTDFTKIKPGAIHRANGGYLVLHIYDIAKNFYVWDKLKRVLRNKEI
                     VVESLSKNLGMGNSETLQPERIPVDIKLVLIGEPIYYYLLYNHDEEFQKLFKIKADFD
                     VEMDRTSVHIRDYARLISSVCSSEKLCHFDPGAVARVVDYGSRMAEDQRKLSTLFNKL
                     VEIIYEANGWACYEKAEIVRAEHVQKAIAEKKYRSAMIEEKIQEQIDQDSLMINVFNR
                     KAGELNGLAVYAMGDYSFGKPVRITAKTFMGEKGLVNIEREIQMSGKIHSKGVLTLNG
                     YMGAQYAQDKNLSLSASLTLEQSYSGIEGDSASSAELYALLSSLANLPLEQGIAVTGS
                     VNQNGEIQPVGGINQKIEGFFQVCQRKGLNGRQGCMIPQKNLTQLMLDEAVVEAVQNG
                     QFHIWAIATIDQGIEILTGMPAGQRDDKGRFPPRTVHFLVDEQLSAWSRKGRRGETGS
                     SDRGEEVSRAVRLRRRRR"
     gene            15420..15728
                     /locus_tag="P5549_04140"
     CDS             15420..15728
                     /locus_tag="P5549_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12292.1"
                     /translation="MQRKNRSGLVLVLLLAMMTLSGCKIDFQKLWQKTQPSEPPLFHV
                     EMHFANGDVVVGYVKSMGIEDDGIIYNGGSSLSYIYNEQGTIVGSLNYARLEYMQLVQ
                     "
     gene            15839..16027
                     /locus_tag="P5549_04145"
     CDS             15839..16027
                     /locus_tag="P5549_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2197 domain-containing protein"
                     /protein_id="HRW12293.1"
                     /translation="MKMLAARCLMCGKSYQLDEDFKDFKKLSEKNLPEVTFICDLCSN
                     KVRYESDEQRKQKKPTSN"
     gene            16041..16796
                     /locus_tag="P5549_04150"
     CDS             16041..16796
                     /locus_tag="P5549_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRW12294.1"
                     /translation="MERIKIINGRGEKLAALVFQPADEPCLVVIICHGFRGGKENGGR
                     IFKFAKRLNQRGAAVIAFDFGGSGESEGLFFDMTLSSQADDLRAVIDYAGQRFAAPMV
                     LLGRSFGGSTVLVEGAGDERIAGFILWSTPVFMEETFAAMLPDEYNRMKSGQVVAIRD
                     DFGSYQLGPGLAADLARHNMNHYLQAIGKRPVLLVHAEDDELVSAENARYMQKQLTNA
                     SLNLVEEAGHRFLDKTAVREDMTLAWLEELFLQ"
     gene            16817..17404
                     /locus_tag="P5549_04155"
     CDS             16817..17404
                     /locus_tag="P5549_04155"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HRW12295.1"
                     /translation="MDFSSIESTVHYIKSFGVYGPAAAFILFFVQAVAPIVPYIIIAG
                     AAGMIFGKWIGFLLAWIGAIAGAWFLYWISDKIAGNLLVVKLQHRYALDLTRIDHRHI
                     FWVLLACRIFPVVPTPIINIGSALAGVSPKIFLSSSAIGKMPWAFVYVALGNYLMQTK
                     NISNTLTVIGLILLISFIGITYLRKLLPKYYREKQ"
     gene            17626..19458
                     /locus_tag="P5549_04160"
     CDS             17626..19458
                     /locus_tag="P5549_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12296.1"
                     /translation="MSANNFILDTRDHKFVLKEWLDVDKVLSFDAYKDYYTKEDADTF
                     IELVYKMCKDVIAPANGECDEIGNKFVDGKVITPEASKKAYHTIVDAGLGPQFGDREA
                     EGRMPMYLGSALCEMVINASPAISTLYGLSMGNGAVIANFATPELKERFLPKMFAGEW
                     GGTMCITEAGAGSDVGNASTKAYPTDTPGLYKIKGGKMFITAGDHDICENFIHLVLAR
                     VEGAREGTGGLSLFIVPKIWVNEDGSLGAPNDVNTVGIEHKMGMKGSPTCTLAFGEDN
                     NCYGFLIGNPPDEKGKGEGMAQMFQMMNEERLGTAVLALGTASAAYQQSLAYSKERVQ
                     SAKLTDMKGGGVRIIEHEDVRRMLMFQKSIIEACRAMIMKTYYYIDLMHDSPDPEERE
                     FADAMFHMSNPLCKAYTSDMAWQTICEAIQVYGGYGFIEEYPCAQYARDVKIFSIWEG
                     TNYIQALDLCARKMTMKKGKVMMTFMKEIGKFLAINKDAAGFADEIKILNEAFTDYQG
                     ILGLFNGFMTSGKINMLGVFATRILHATAMVYAGTLILDQAILATKTLAEIGDDHFDA
                     TFYKGKIASARFFIKNIVPQVFNIKRVMEIGDSTCVDIPEDCLR"
     gene            19928..21760
                     /locus_tag="P5549_04165"
     CDS             19928..21760
                     /locus_tag="P5549_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12297.1"
                     /translation="MSAGNYIYDMRDLQFILKEWLDMDKLLGFEAYRDYYSKDDLDSI
                     LDVNYKISRDVIAPANVECDEIGVKFIDGQVITPEAIKKAYAVVMEAGLGPQNADREV
                     EGRMPLVMNAAIGEMQINASPAVTTYWGLTMGAGGVIQSFSTPELKERFLPKMFTGEW
                     GGSMCITEAGAGSDVGNASTKAFPTDTPGLYKIKGGKMFITAGDNDIAKNIVHLCLAR
                     IEGARRGTAGLSLFIVPKIWVNEDGSMGESNDVTTVGIEHKMGLKGSATCTLAFGEND
                     KCFGWLIGNPPGEDGKGEGMAQMFQMMNEERLATGLLGLGVSSAAYQQSLAYAKERTQ
                     SAKLLDMKGPAVRIIEHEDVRRMLMLQKAYTEACRAMIMKTYYYIDLSHDSPDPEDKE
                     FANLMFHMTNPLCKAYATDVAWDSIAAAIQVYGGYGFIEEYPVAQYARDVKIFSIWEG
                     TNYIQALDLCARKFTMKKGKVLMTFFGELGKFLAANKEAAGFEEAMQVFADAFTAYQG
                     ILGTFNNYMTSGKIQMLGLYATRILHASAMLNCAMLILDQAILASKKLAEIGPDHYDA
                     SYYTGKIASAKFYVMNILPDVFTIKKVFEMGDTSAVDIPEEALR"
     gene            22371..24203
                     /locus_tag="P5549_04170"
     CDS             22371..24203
                     /locus_tag="P5549_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12298.1"
                     /translation="MSAGNFIYDTRDQLFILKEWLDMDKLFGMEAYRDYYSKDDIDSF
                     IDVSYKICRDVIAPANQECDEIGLKFVDGVVVTPPAVKKAYSTVMDAGLGPQSADREA
                     EGRLPLVVCAAYGEMQINASPAVATYYGLTAGAGGVIQTYSSPELKERFLPKMFSGEW
                     GGTMCITEAGAGSDVGNAATKAFATDTPGLYKIKGSKMFITAGDHDIAKNFVHLVLAR
                     IDGARKGTGGLSLFIVPKIWVNEDGSMGDSNDVLTVAIEHKMGLKGSATCQLAFGEND
                     KCFGWLIGNPPGEDGKGEGMSQMFQMMNEERLGTGMLALGTANAAYQQAIAYTKERVQ
                     SAKLTDMKGGAVRIIEHEDIRRMLIKQKACVEAIRAMIMKTYYYIDLSHDSDDPEERE
                     FGNLMFHIGNPLCKAYASDMAWDLICDAIQCYGGYGFIEEYPVAQYARDVKIFSIWEG
                     TNYIQALDLCARKFTMKKGKVLMSFFGEVGKFIAAAKEAAGFEDLIKLYEEAFAAFGG
                     IMGSFNGYMQGGKIPMLGLYATRILHATSMIYCGMCILDQAILAAKKLAEVGEDHYDA
                     GFYKGKITSARFYIMNILPDIFTIQKVIAMGDTSAIDIAEENIR"
     gene            24573..24827
                     /locus_tag="P5549_04175"
                     /pseudo
     CDS             24573..24827
                     /locus_tag="P5549_04175"
                     /inference="COORDINATES: protein motif:HMM:NF018965.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25144..26484
                     /locus_tag="P5549_04180"
     CDS             25144..26484
                     /locus_tag="P5549_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12299.1"
                     /translation="MYQGEYRRKLVLAEEAVKCVKTGDIVDYGFFNGKPVLCDQALAK
                     RTELRDVKVYCAVTIPPVPEVAKVRDCFTYHDWQFSKIARVLQSYGLCYYSPILYHQA
                     PSLYRDGIAEHRNVAIIRVCPMDKHGWFNLGPQNSETMANAESADYVIVEVNKSIPVC
                     LGGAEEAIHISQVDYIVEASDDDILPDIPGAVPSEEDKQIARHIMDYVHDGSCIQLGI
                     GGMPNAVGKMIADSDLKNLGGHSEMVVDAYMDMMQSGRMNGHAKQIDRNRVAYTFAIG
                     SHKLYEYMDNNPAFASYPVNYTNDPRIISQLDNFVSINNCVEIDLYTQVNAESKGPVQ
                     ISGNGGMWDFVLGAQFSRDGKSFLCMTSSYKDKDGNLHSRIVPTFTPASIVTIPRQQV
                     DYIVTEYGAVQMRAKPTWQRAEKLIELAHPQFRDDLIKEAEKLNIWRKINKEDV"
     gene            complement(26600..26770)
                     /locus_tag="P5549_04185"
     CDS             complement(26600..26770)
                     /locus_tag="P5549_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRW12300.1"
                     /translation="MFALIGNFGPWEVGLILLIILMIVGPGKLPKVAESMGKAIKGFR
                     GSVKDDDKREIS"
     gene            complement(26825..27604)
                     /gene="ccsA"
                     /locus_tag="P5549_04190"
     CDS             complement(26825..27604)
                     /gene="ccsA"
                     /locus_tag="P5549_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRW12301.1"
                     /translation="MISIEHLLIYVIFLLYLASMVFYWAARQTPAWMRYASWLAMFGL
                     LCNFLALAYHVAYSHRLPLTNGYEFMMSFVFITVLSHLLYEKRSRHAAAGAAVMLINV
                     LLMVSIMVLMPQQIGQAGPLMPALKSPWLISHVLTAIAAYSAFALVSGLALVKLQANN
                     PADDHDIYRITSLGFVMLTLSIVLGAIWAEQVWGSYWTWDPKETWALITWIIYAIYLH
                     LHRQRGWRGNKARILLIAGFVLVLFTFFGVNYLLPGLHSYA"
     gene            complement(27601..28599)
                     /locus_tag="P5549_04195"
     CDS             complement(27601..28599)
                     /locus_tag="P5549_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRW12302.1"
                     /translation="MRFYNYISSMNAGLILLGLIGIASAIGSSLWPEMFFKTVYFRLL
                     LLLFFVNMALCTFNSIKKLVAGKKRILHNKRQMLRSICLIMLHSGIVLIIVGASLNSW
                     WGHSVQLSILQGDTVEINRIIPVNTPFQVKLLDFSIPFNEDGSPAQYYSTLQVASSGQ
                     NELQQTISVNHPLTFAGVKFYQNSYGYLLNAGAEDGETSKDLLVQDGDWISFESTRRT
                     VKVFKYIPHFDPSLGMQTKSLEPKNPRVIYTVYEGEHMLGVGTAPFKERIKIDEQTYI
                     TFAQVRPYSILTVKTDPGMPLTAAGGILLMLGISIVLLMPRKKPNRSQSDKEDIAS"
     gene            complement(28625..29200)
                     /locus_tag="P5549_04200"
     CDS             complement(28625..29200)
                     /locus_tag="P5549_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRW12303.1"
                     /translation="MTDNHHENKSFREKYLNKKVILIALSIAVVLGVMVAAGLLKASE
                     NPSFCKTCHIIRPFYESWNEGIYLDHKHALENIECLDCHHRTIPEKAMEGLNYITGNY
                     ELPLQAALGDRAFCLDCHKEGGQGEGWDEIVAATDYEDSNPHDSHHGEQDCNLCHNMH
                     GKSKPMCVECHLWGWYDELDESWSTEFEEEI"
BASE COUNT         8040 a         6583 c         7551 g         7290 t
ORIGIN      
        1 tacgatatgc gttcatcacc ggcaatgaga gcagtgtgtt caggcttttg tgctgctgct
       61 tgcttcaggt agtccgacaa caacttcaaa gctccttctg tatggctcat taaaccaccc
      121 tcctttttcg ttcgcttggc caacaatgga aaaaagaaaa tacaaccctt ttttggatat
      181 tcattcctgc gaataaataa tatgcgattg aaagatcggg tatgtatata tttttgtcta
      241 aatattcaga aaaatcaatt aataatttta atctcgtccg caacgatgac aggcaaagag
      301 caatctatta tgatttaatc aattgattac attgacaagg gcgggatgat tttgccggaa
      361 acgataggag caagcgccag gattgcagtg attgctattt ttctcttgct gtggggtgga
      421 ttaaattatt atttgggctg gcgcggctgg cactattttg gcgattatat caatccctat
      481 agaagtcttt attgggtttt actgatattt ctggcctcta cttatttttt gggcagatgg
      541 ggcaaccacc attatcccgg cgctgtcagc gatgcgctga tttggctcgg ttcctactgg
      601 atggcgtttc tgttttatgc gctgctgctc aatgtgctga ttgatctgct taaggtgttg
      661 gacagggtct ggcggtttat gccggactgc ctgcatcatg ctgccggccc gattgccgcg
      721 gccttatttc tgctgatcat tcttggtctg gtgtatggaa cagtcaatgc ccatcgaccg
      781 gtggttaccc ggtatcatat tcatgttgaa aaaccggccg gggaacggga aaagcttcat
      841 attgtaatgg tatcggatat tcatctcggc aatattgtgg gtcgggatcg tctggagggc
      901 atggtgaaac agataaacca gatgcagccg gaaatcgtgt tgttggcagg agacatcatc
      961 gacggtgaca tcagaccttt tgaacaacag gccatggggg aactgctgag aggaattcat
     1021 gctccgttgg gggtttatgc tgtgccgggc aatcatgaat acctgggcgg acaataccga
     1081 cagcttttgg cggcactaca agacagcgga gtggttgtcc tgcgggatca agttttgttg
     1141 ctggacggtt tttatctggc aggccgcgat gataaaatca gccggcaaag aaaaccgctg
     1201 gcggaagtct tggcaggggt ggatcatgca tatccgatca tactgctgga tcataacccc
     1261 gtcgatatac aagaatccct ctccaaccga gtcgacttgc atttgtcggg acatactcat
     1321 gcgggtcaaa tgtttccgtt aaaattcttt acggcaagga tttttgaaca ggattggggc
     1381 tatttgcaaa acggtgcgtt gcacgtcatt gtttccaatg gatacgggac ctggggtccc
     1441 cccatccgca tcggtaatcg gccggagatc gtagaaatat taatcacttt tcaaggaaga
     1501 taatcggatt aaagcatact tgtacacaga atcagcggga gttttcctgc caagaggaga
     1561 aagaaaaaga aaaatgaacg gaatggagaa tcaggaaggt attaccatgt tttgctggta
     1621 tcgttttaaa aactgacagg gggtttgttt atgttttcgc gttttaacat tcatccgcat
     1681 cttattagaa agcgaagaag gatcggtttg tttttgacag ccttgctgct gttgcaggtt
     1741 ttaacacccg ccttgctggc gtcaccggtc caatccgcag aagagatgca gccactgccg
     1801 ggcaggatcg cattatttca tgcaagcgcg gtcccggcat tggtttcacc gggccggcta
     1861 tcaaccccgg aggagaatgc caaccagtta aataacatca cgacgcagaa ggatgcgtta
     1921 tcctcctttg catacataaa cgacaatgcc gaaatcctgg ttgcagaagg caatcaacaa
     1981 gacataacat tacctcccaa aacgcctcag ccagaggtgg ggatggcaac aaaacctgct
     2041 gttgctgccc gggaaatcat tgtatgttac gagaatgatg agtctttcgg gatgcgtccc
     2101 cagtctctgg aggaagagaa cgggacgccc aaagagaata ctcggctgtc agttgttgct
     2161 ttgtcagcag aggatgatcc ggtcagtgtc atcgaacagc tggaagagga tccggcagta
     2221 aaatatgccg agcccaacta tatcctgcaa gcccaggttg cgcccaatga ccagagctat
     2281 gttgatcaat gggcgttgtc tgagatcaat gccatatccg cctgggatac agcggtagag
     2341 gctgaaggtg ctctcgcgcc ggttacggtt gcggttattg acagcggcgt ggatggcaat
     2401 catgaagatc tggcgggaaa aatactgcct ggatacaaca ccgttggcgg ggttaacagc
     2461 gctgacagtt ctgatgactc cggtaacgga cacgggactc acattgctgg cgtcattgct
     2521 gcgcaaacca ataacgtgac tggggtggcc ggagccagcg gcaatttgcc ggttgatgtc
     2581 cttcctgtca aagtgctgga caaggccggt tatgggacca tgtatgacat ttccatgggc
     2641 attcgctatg cagtggactg ggcagagatg aacaaccgca aaatcgtgat caatctcagt
     2701 ctgggagctc gtctgcccga ctatcccatg actttggccg cagcggtaca atatgcacaa
     2761 gataaaggag cgctggtggt ggcggccgcc ggtaacgaca aggccggact ggagagcttt
     2821 tatcctgcat gtctgcccgg ggtgcttgca gttgccgctt caggcaaaga tcatcacatg
     2881 gccggttttt caaattatgg cgatattcct gctcccgggg ttgacatcct gtcgaccctg
     2941 cccggcaacc gttatggcag attaagcggg acctcccagg cggctgcttt tgtatccgcc
     3001 actgctgcca tgcaatggtc ggcctatccg gataaaagtg cggcccaagc ccaggaagcc
     3061 ttaatcagcg gtcgcagtga ctacccggag gggtgtaaag tgtactattg gggtacggtt
     3121 tgcagttacc actattatgt cctcaatgcc aaccaggctg tctacaatct tggtgaggaa
     3181 caaaacattt atgataccat tgaattcgcc aacaccgcct atgatgctca tttgtccggt
     3241 gaaattcctt acatcgccaa agtcagcaat ccagccaagg tggcagaagt gcaattctgg
     3301 ctgaatccgt atggggaaag tattcatttg gggacagtaa ccggtccgct ggccagcggg
     3361 atttacacgt accagtggga cagcactgct ttactcaatg gcaagccgct cccggacggg
     3421 gaatacactg tggaagcccg ttccatggat agctctggcg ttcaattggc tgcgtgctca
     3481 tatgatttca aacttgccaa tgaatccagc agcggcttgc atcttgccgt aaaaaaaccg
     3541 gatgcttcct tggctgccgg agccttggtg ggggtttatc atcgttattt taatgaagac
     3601 tatggacaaa ttgtgtatga atgcgtttgg gaggatgagg ctgatctcca aggtcagatc
     3661 aacatcccca acgctatggc taaagacggc aatgactacc tggtcacagc actgggcacg
     3721 gaaccgaatt tcttctatta tgaaacgctg cgggcgcccg gcacttatat cctggacggc
     3781 actgccagcc aggcagtggg tctgaaagga ttgaaaacag acggtactgc gctgggcaat
     3841 gccattatca tggctgatct gctggaagcc agtttcgcgg atgccaatcc ttcccctggc
     3901 gttgcctacg agctgcagga gaaaaaagta gccgtactca acgccgctgg tgaagcgaca
     3961 ctgaatctca gcccgggacg atacaatttc cgtctgctgg atgaagaaag cacatattac
     4021 ctggtgcagc atgatgtact gattgataca acaaccacga cgatcgattt cactccgcag
     4081 tcgggtgatg tagcaaccgt aaagctggct tctgacagcg attattacag ttcaactttg
     4141 ctgctcaagg acagcggtga tgattattac ggttttaagg aaattaacag cgacacttgc
     4201 ctgacggtta ctccgggcag ttacaatgca gttataaaag caatcaaacg tgatgcggaa
     4261 aataatatag aatggaactg gggactggaa tctccgaata tggacctggc agcaggtgac
     4321 aaaaaagttt tgcagtttgg tacaccgctc agcggctcat tgagccaatg gcaggatgaa
     4381 atgcctgtcg ccggggagag ttactggatg aatccggcct ttaatgatgc ttttggcaac
     4441 accaccaccg ggattgccta tcagtcctat acaccagcat ctttgccggc gtctgcccaa
     4501 agcagcggtt ctgtcatagc tcgaatggtg gccgacaaac cggcggaaaa gactgaccca
     4561 tctggcagta caccgcagga agaacaaatc tatgtttatg aacgcagccg aggcgacttc
     4621 gttttaagta cccaggcagt aagctacatc cgtcctgcga taagaattta tgaccctcaa
     4681 ggggcacagg tcatgcccca aacttcttat gaacagacct gcagttattc ttttgccgag
     4741 tggtatattc ctgcgcagca atcacccggt cagtattctg cccaggtata tctggatgca
     4801 ggcactctga ccaacggcac ctgtgaaagt ccacctttcc tctttacggt gcaggctgaa
     4861 gcgtcatcaa atatcgaacc gagcgtcaat gtaacgctgc tggatcggat gggtgaaccg
     4921 atgacaggag ccagcgttga cttaatgcaa ttacatgacg gacagtatcg catcctggat
     4981 agcagtaccg ctgatgaagc gggccaggca tccttctatt cggaaattgc aaaaaccgga
     5041 tcctatgcgc tgaaaatttc cgggatgagt gccgatcccc tggtgattga ttcactcagc
     5101 gaaccactgg ctctgcttgt gcctttgacg gcgggcaccg ttccgctgaa tattatggtg
     5161 gatgcttccc ggcaggtatt acatcgcatc agtctggcgg cccaggatca aacccttcaa
     5221 ccgattacca gcgacagtct gtactattat ggctatcgtt tggatgagca agggaatgaa
     5281 tccttggttt ttaaaactcc ctatgaaggt gaaaatgtgg ccgtttggct tgccccaggg
     5341 gagtatcgtt ttgaagccag tgtaaacaac cagtattcga tagcgcaatc gctgggtaca
     5401 tcagcaccgg caacgcagga tctgaaggcg caggggtatc tggatcctct ctatttgctt
     5461 acacgtgagg tccaggtaag tgcttccggg gaaatcgttt taggcggcag cggattaggc
     5521 aaattagatc ttttactcac cgaattagga gactcctatg actattatct gggcggagcc
     5581 gccttttacc ggcaaggaca gacagagaca acgctattca aacctttcaa ggatcagtcc
     5641 atctatctta cgcccggagc ctaccaggcg gaagccgtac tgatccgcaa tcattatgac
     5701 ggacaatggg attattggct gggccagcaa ctggacattc aagccggcag tgaatcaatt
     5761 tgggaaatga atgacaactt cacaactcaa atcaaccttg ctgatgacac ttatgcgctc
     5821 aaccaacaac tgaccagcag ccacttgatc aaagatgaca aggacaatcg gctgctggcg
     5881 atgggtttga attttgaatt ttacaacact tatttgccca gcagtatgag cgagcgctca
     5941 accgctgccc tgacggtgca gaatcagccg ctggaaaaaa tggctccgtt ttttataatt
     6001 aaagatccac aagggcaaga agtatatcgt tatcgtgatg cggatcccaa tttcaatgct
     6061 ctttcttact ggtgctggaa tgaagacggc cgtatcacag ccctgaaaga acagggtacc
     6121 ttttataccg gcaagtatca gatcccggag aatgccaccg gcgggactta catggcagcc
     6181 ctggagttgg gcgtcggtcc gcaggggatt atcacagcac agaccccttt cagtgttgca
     6241 gcagtaccat cggcacctac cctggaggcg tttgaggctc cgaccagagc cgagaagatt
     6301 agcctctccg gcaatgccct gcctgcggcg acagtctacc tgcagtatcg attcaaccag
     6361 ggagaggttg tcgcgctggg gaatgtcaat gccgatgcag aagggaaatt tacttttgct
     6421 gatatcagcc tggctgatga gggaagctat gaattcagtg catacgccag cctgggtggt
     6481 ctgacgggag aagtctgttc accgcaggtg ctggtggtgg accgcaccgc tccaggcagt
     6541 ccgcttgagc ctgtcgcagt tggccaggat ttcagccata ttttgatcag ctggacagcg
     6601 ccatctgata cggacattga tcattatctg gtttaccggg aagataccct cctgggaaca
     6661 gttgccgcaa atgctctccg gcagttcctg gatacaggac tatcctcgga aatcgattac
     6721 ctttatgaga tcatcgcggt tgaccaggcc ggcaaccagt cggaggcaac tgcggtcaac
     6781 gggcgaacgg catccagcgg agatcagatg aaaccctctg cgcctggtga gttgaggctg
     6841 aatgatgccc cgacctctgc caacgtaacc ctgacctgga cagccgctac ggacaatatt
     6901 gccgttgtcg cttacaaggt ctggcgcagc atggatggag cagaaccagt gcttctggcc
     6961 acgacagagc agctgagttt cagcgatgac aatctgctgg cggagacaac ctttgtttat
     7021 acggtttccg ctctggatga agccggcaat gaatcatcgc ccagcaatcc gctgatggtt
     7081 accaccccgg ctttacagtt tacttccatt agttattcgg tgccgagaaa catgaatggg
     7141 ctgattaaag aagaagccag tattaccgta aatttggctg cacagtataa acgcaaagct
     7201 acggctgaat tgatctatac caattggtat gatgaaaccg gaacgctgtt gaatgaacca
     7261 caggaacgaa cattaatcat ggaacttccc atattccagg aatatacctc tctgggaaca
     7321 gcctcctacc gcgggagttt tcaattcccg gctggaacag agcaattgtt gtcagtcaag
     7381 gcgacgctgc gtgatgaagc ctcccatagt gtgaccgcaa cagctgacgc tctgcctttg
     7441 ttctttacag gcaggcttga attgatcatg gctgaagaag ctttgacttc aggtttgtta
     7501 gatgatgcaa ccgcggaaat ctggagcgag acgaaaaaaa gtggcaccaa gacaagcctg
     7561 tccgcagaag gcgttttcac tttacggaat ctgactccgg ccgatgacta ttcgttgcgc
     7621 atcacaaacc agaaagggca attgttgaaa gagcaaagcg gcttgcacgt aattgcgggc
     7681 caaaacgcac agatccccat gacgacaaga ttaccggctt cgcttgatgt gcaggttatt
     7741 gatgcccaaa gcggtgagcc cgtaccaggg attgcagttt actttaacca tgcggatgaa
     7801 tcacctcagg gcatgggttt gacggatgaa gcaggatggg taaaaaacaa tcaaggccag
     7861 cgcccggcca tccccattgc cttgtcggaa accatcctgc agggcagggt tcaatttact
     7921 cccgcgcaac aagcggtata ttcccatgca gaaagactgc ggacaacctt ggagccgggc
     7981 ctgaatcaag cgatgattca attgtacaaa agaccggtgg gtatcctgca gggaaaagtg
     8041 acagatgatg caggcaaaga tcttgcagag gctaccatta cagcggttca gtatgtggag
     8101 gaaagagcag tcagcaaaac cgtattaact gatgccaatg gaaattattc gttggaatta
     8161 ttgtccggga tcaatagtga gatcagcttc cgtcaccggc gtaccagtcc ggtagaggat
     8221 caggaagttt tcatcaacga taacacggtt ttaaatatgg tgctgcctgc ccaggcggca
     8281 gtcaccctga acgtatttac caaaaccgct gagaatgcag aaccggtacc ccttatgctc
     8341 gactggcggg tggcggttca tttccgaatc aatattcaaa atctcaggac cagaagcgtc
     8401 gcttcctgct attcaacctg gcccaacgtg atcctgcctg atgggcaagc cggtgatcag
     8461 ataaaaatca ctgtcgacgg ttatgaaggc ggttattcaa agcaggagca gatcgttacc
     8521 ctggatgcat atcgctatgc agaagctgat attaccctta cccaacaggg gaaattacgt
     8581 gccagcatct atgacacaga cgggtcaaaa cagtacggtg agcgtttaaa tgtaatgctg
     8641 tatcgctatg atgaaaatgt ctggaaagga gccggctcac tgtacgagta cggcggcggg
     8701 gaaaagactt ttggaccgat accggagggc aattaccggg gcgttcttta ttggggaccg
     8761 cgcctgccgg tcgtccaaaa cggccgggac gaattcgcca tcgaagggtc caaaatcatt
     8821 gaagatatcg taatccgcaa tggagaaaca accgaactgg gcaacattgt cctgcctttc
     8881 attctgccct ggcacagcca gagcggctat ttcaaaggcc aggaaggcaa cagtttctcg
     8941 gccagtatgc gcgaggcagc tcccaacagc gtcgtgacgc tgcgggccag ctatcgttac
     9001 agcggcagtc agagcaatca gccccaggat atgaaactca gtctcagcat cccccgcgga
     9061 gcgaccctgg ttcctgacag cattcaggtg agagcggaac agggggcggc ttcagttccc
     9121 gtcatcgaag atacgcaaat cagtatgaat cttattccgg aagcggaaac aaattcatca
     9181 gtctccggcg tggtcagtta tcaggttcgc ctgagtgcct atccggattg gcctacagca
     9241 ggtgcccggg cctggatgga attttcggcc gggaatcgta cttacaatga agatctgggc
     9301 aatctcgaga tcgcaacgcc ttatgtgtcg gtagaggcac cttcgccggt gatcagcaga
     9361 aatattaaaa tcaccgggca agcgctggct gatcagcatg tgcgggtata cgacggggaa
     9421 tatttcctgg gtgaagccac ggccaatcgt tatgggttat ggcagatgca agtgcttctt
     9481 cctgatcggg gccagccggc catgcacttt ctcaacgccc aaaccgggga gggggaaagt
     9541 cttattggtt ctctgccggt acgggttgtt ttcgatccca atcatccgac catcatccgc
     9601 tttaccatgc agcagacgga tggacgattg gtggaaatcg atccgcagct gggggtggct
     9661 tcgttcccat acgtatttgt gccgggcatg aatttcacct tcaacatcac ctttaacaac
     9721 catcaggcgg tggaagaagt tgccctgatc ggcagcaatg aacgttccga ccgaacctat
     9781 ccggcagtct ggaacgaagc cgaacaatgt tattcggtca cggccaaatt tgaatactat
     9841 agcggcacac caggggatct atacgtcagt tacaaaatta aggatacgcc ttatcagccg
     9901 gtcattatcc cttccagcga ggaagaaata agagcactgg acgaggaact gcgccgggct
     9961 atgccgcagc cgtggcggga tgcaacggta acggtggatg accctggaca ggtgactgag
    10021 ccgggcagcg gacctaatcc gagcccgcag atcaacatcg ctttaaacga taatccgcag
    10081 cagttattga agatcacttt tggctataac gagatcagtg attttgctgc caatccagcg
    10141 ataccgatga ttggtatctc caatggcagc agtgtttcca atctgcagga cgtaattacc
    10201 gagcttccca atggcgggtt ccgtctgcgc cggagctgcg tcgtcccggc tagtgtactt
    10261 aatgattatc ttgccgccgg cagtcagagt ttttccgccc aggccagcaa ctcggttgag
    10321 ttgtgggtca tggccgaatc gccttatgaa ggcgggatgc agttggccga cttgtatgat
    10381 acggtcaaat cgggttttga ttttaaagac aaactggatg aattgagtgc ttttcaggat
    10441 caggcggcca gaagctgcaa cagcggtgcg gcaggatatt acaatgacat ggcggaacac
    10501 ctggccgata ctgccatgat taatttatcc atcaagtata ccctgcaggt gggcgggata
    10561 gcgttgggac tgaccggtgt tgggcttatt ggtaccgccg cagtaggagt aatcagcaat
    10621 ttgctgacgg aagtcggcga agccaaatgg gaacagaatt ttaatgattt aaaacagcaa
    10681 ctgagcaatg atgatgaatg caaggacgac gagcaagacg acgacaatga tgaccgcgat
    10741 gacagcgacg accgggatga cagggatgac cgggattatc ccaaaaagaa aaagaaaata
    10801 gccaaaccaa aatatatttg ggatcccagc ggctatgtct atgaagccgt tgttgataat
    10861 cgtattgaag gagttactac taccattttt gaaaaggata ctgacagcgg gcagttggtt
    10921 ttctgggatg caaccgagta tctgcaggaa aatccgctgg ttactgacag tcagggacgc
    10981 tatgcctggg atgtaccggc cggatactgg caagtggcat atgaaaaaga aggatatcag
    11041 actgcttaca gttcgctatt ggaagtgcct ccgccccagt tggatgtaaa tgtgcctatc
    11101 gtttctcttg ctccccccgt ggtaaccggg gctgcggccg ccggcggcgg aagctgcctg
    11161 gaaatctctt ttgacaagta tatgcgttta aatagtatga ccgacactac gattgccgtt
    11221 ttgctgccgg gacaagtcga tgagttcggt aatcctgagc gtatcacggg aaccatcagt
    11281 gcaatcaacc ccagtgatga tcccggtgct gccggggcac aacttgcgaa aacaatacgt
    11341 tttacacctg cacaagcact aacggtaggt gaagcagtga atctgcaggt aagtcaaatg
    11401 gtgcagagtt atgccggacg gacgatggcg gcggattaca gcacgacgct tacggtaccg
    11461 gcagtgactg tgccggaaga gcctgctgtt cccggcaacg gatcatccag cggaggaaga
    11521 agcaatccgc ctgtcgttga tcagctgaac agtatcagtc aaacggtcag tgttaccagt
    11581 ggcgtccagc taagcagcaa agatgatgca gctgtggtta ccatacctgc tggtgcagta
    11641 tcagccgatg gcatactgaa agtatccctg gccagcaagc ttcctttcct gggcagtctg
    11701 acagcgggca gtcagggcta tgacatcgtg ttggaaaata caacactggg tggccgggca
    11761 accataagta tttcttatga cccagaacgt ttcaggaaca ttgcgcccga acagatcggc
    11821 atttattatt gggacgaaag cctgagcaaa tgggtatatc tcggcggtat agtcgacaaa
    11881 gacaaccacc gggtcacggt tactgtgaat catttaagtt tatatgcggt tttggccgac
    11941 acttccatac cggttttcaa agatatacac actcactggg cttatgcgga gatcgtccgg
    12001 ctggccggtt cgaaaataac cggcgggtat cccgatggaa ccttccggcc agatgccaac
    12061 atttcccggg cagagtttgc caaacttctg gttgaggcca tgggctggca ggcaggtgaa
    12121 ggaatagctg attttgcaga tgcggctgat cttcctgtct gggcatcggg ttacatcgcc
    12181 accgctgcag ccaaaggcgt gatcaaaggt tatgctgatt cgaccttccg ggctgatcag
    12241 ctgatcaatc gtgccgaaat ggccaccatg gtgattcgtg ccctgtccgt cggccagaat
    12301 aaccagacgg aaacgatcgg cagcaacttt acagatcatg cagcgattcc ctcctgggcg
    12361 caaagcgcag tggaattggc ggtgaaagcc ggcatcctca aaggcttgcc cggcaatgtc
    12421 tttgggcctt tgcaaaatgc cacccgggca gagaccgccg tgatcctgac ccgcatgctg
    12481 gataatccca aactggcaac agttgttggg caaacccaaa cgatcaatgc cagcggtgtg
    12541 gtagcaatta gtaatatttc cggcccgcat tatgaactga gaagctgctc cagcagtcag
    12601 acggctaaat atgtattaat ccccaacagt gaagtcttgg ccgaacggct gaaaaccttg
    12661 ctgggacaga ccgttgccgt agctggaagc ttgtcagagg gagctgatat ctatatgcgg
    12721 ggccccatgt tgaaggtaac tgaaattaag gtgatttcaa atcccggcgg aggctgaggc
    12781 caataaaccg aagccggcgg cttctcccgg ataaaaatgc aagagctgtc ccggatttca
    12841 gtgtaagaat gcgggacagc ttttttgcgt tctgtcaaaa ttttaacggg cctagatttt
    12901 gcaggtattt acgggtaaaa ttacaaatat aaagtgaaag agaatgaata tggaggaatc
    12961 gcttttgaac aacgacaggt tgaaagtgaa agccaaagac ttgcgtaaga attgcaaccc
    13021 ttctattttc aaatttcaaa ccacggcgga gataaaaccg ctcaaaggca tcatcggcca
    13081 ggagcgagcg gtgcgttcac tggacttcgg cttggatatt aaaaatgaag gttataatat
    13141 ttacctttcc ggtgcctttg ggactggaaa aaccaccttg gccaaggaga tgctggagaa
    13201 gaaggcccgc cgtaaaaaag tgccgccgga ctggtgctat gttcacaatt ttaaacagca
    13261 tgactctccg cgtgctctgc aattaccggc agggatgggg cgggcgttca aacaggatgt
    13321 ggctgaggcc atgcaggagg ttatgcagca agcaatcaag gcgttggaga gccaggatca
    13381 caataccaga aaaagtgtca tcatgaatca attcatggaa gaaaccaacc gcatgtatct
    13441 gcaattggaa gaggaatccc gtaattacgg ctttagtatt tcccgcacgg ccaacggagt
    13501 cagcagtgtg cctctgaaaa acggtgagat tctgagtcag gaagactacg tgtccatgtc
    13561 ggaagaagag aaaatggatt tgctgaaacg cagtgcggta atgcaggaaa gaatcaacca
    13621 atcgattcgc gtctataaag aactggaaaa ggcagtgaaa ttgaacctca aggacctgga
    13681 aacagaaacc accctgaaag ccagcgaacc attttttgag aaactgctga aaaaatacgg
    13741 gtcatatcca gatgtatcgg attacctgca ggatatgcat caggacgtct taaaaaacct
    13801 tgatttgttt attgccgaag aagatacctc ggggatgggg ttcttccgta atatggataa
    13861 aaaaagtgcc ttgcggcgtt atcaggtcaa ccatttcgtt gattgctcag acttgcaata
    13921 tgcaccggtg atttttgaat ccaatcctac ttacagcaat ctgtttggtc agattgagtt
    13981 cgaaagtgag tttggtgtat tgtccactga tttcaccaag ataaagcccg gcgcaattca
    14041 tcgggccaat ggcggctatt tggtgttgca tatctatgat attgccaaaa atttttacgt
    14101 ctgggataaa ttaaaacggg ttttgcgcaa taaagagatc gtggtggaga gcctgagcaa
    14161 aaatttaggc atgggcaaca gtgaaacact ccaaccggaa cggattccgg ttgatataaa
    14221 actggtgctg atcggcgaac caatctatta ttatctgctg tataaccatg acgaggaatt
    14281 ccaaaaacta tttaaaatca aagcagattt tgatgtggaa atggatcgca cttccgtaca
    14341 cataagagat tatgcacggc tcatcagctc ggtttgcagc agtgaaaaac tatgtcactt
    14401 tgatccaggg gcggtagccc gggtcgtgga ttatggcagc cgtatggccg aggatcagcg
    14461 caagctgagt accctgttca acaagctggt agagatcatc tatgaggcca atggctgggc
    14521 ctgttatgaa aaggctgaaa ttgtcagggc tgaacatgtt cagaaggcca ttgctgaaaa
    14581 gaaatatcgt tctgccatga tcgaggaaaa aattcaggaa caaattgatc aggacagtct
    14641 gatgatcaat gtttttaatc gcaaagcagg cgaattaaat ggattggcag tatatgcgat
    14701 gggggattac tcgtttggca aaccggtacg gattaccgcc aagactttca tgggagaaaa
    14761 agggctggtg aatatcgaac gcgagatcca gatgagcggc aagattcaca gcaaaggcgt
    14821 attgaccctg aacggttata tgggggccca atatgcccaa gacaaaaatt tatcattgtc
    14881 cgccagtctc accctggaac agagttattc cggcatcgaa ggtgacagtg cttccagtgc
    14941 ggagctatat gcacttttgt ccagtctggc caatctgccg ctggaacagg ggattgcggt
    15001 taccggatca gtaaatcaaa acggggagat tcaaccggtc ggcgggatca atcaaaagat
    15061 cgaaggcttt ttccaggtct gccagcgaaa aggtttgaat ggcaggcagg gttgtatgat
    15121 tccccaaaag aatcttaccc agctgatgct ggatgaagct gtggtggaag cggtccagaa
    15181 tggtcaattt catatttggg caattgcaac catcgaccag ggcattgaga tcttaaccgg
    15241 catgccggcc gggcagcgtg atgacaaagg ccggttcccg ccccggacgg ttcacttttt
    15301 ggtggacgaa caactcagtg cctggagtcg caaaggcaga agaggagaga ccggttcaag
    15361 tgaccgtggc gaagaagtat cccgggcggt aaggttgcgc cggagaagga ggtaatacca
    15421 tgcagcgtaa aaacaggagc ggtttggtgc tggtgctttt gctggcgatg atgactctgt
    15481 caggctgtaa aatcgatttc cagaagctct ggcaaaaaac ccagccgtcc gagccacctt
    15541 tatttcatgt ggaaatgcac tttgccaatg gtgatgtggt ggtgggctat gtgaaaagca
    15601 tggggatcga agatgatggt atcatatata atggtggttc ctccctcagt tacatctata
    15661 atgaacaggg aacgatcgtg ggttctttga attacgcccg cctggaatac atgcaactgg
    15721 tacaatgaga gctaatgcga cgggctgagt ccgtcccgat gaaaaaccag gcccatacgg
    15781 attaacaccg ctgatatgca ggaggagaaa atagtaaaga caagaggagt tgatgggtat
    15841 gaagatgctg gcagccagat gtttgatgtg cggaaaatcg tatcagttgg atgaagattt
    15901 caaggatttc aaaaagctca gtgaaaagaa cctgccggaa gtgaccttta tttgtgatct
    15961 gtgcagcaac aaagtgcgtt atgaatcgga cgagcaaagg aaacaaaaaa aaccaaccag
    16021 caattaatgg aggcagacga ttggaacgta tcaagattat caacggcagg ggagagaagc
    16081 tggctgcact tgtttttcag ccggccgatg aaccgtgttt ggtggtcatc atttgtcatg
    16141 gctttcgagg cggcaaggaa aacggcggcc gcatatttaa gtttgccaag cgtctcaatc
    16201 aacggggtgc agcggtgatt gcttttgatt tcggcggcag cggagaaagc gaaggattgt
    16261 ttttcgacat gacgctgagc agtcaggcag acgatctgcg ggcggtcatt gattatgccg
    16321 ggcaacggtt tgcggccccg atggttttat tgggtcgcag cttcggtggc agtacggtac
    16381 tggttgaagg ggccggggat gagcgaattg ccggttttat tctttggtca acacccgtat
    16441 ttatggaaga aacctttgct gccatgctgc cggacgagta taatcggatg aaaagcggac
    16501 aagttgttgc tataagggat gatttcggaa gttatcaact aggtcccggc ctggcagcag
    16561 atctcgcccg ccataatatg aaccattatc tgcaggcaat cgggaagcgg ccggttttac
    16621 ttgtacatgc tgaagatgat gaactggtat cagcggaaaa cgctcgttac atgcaaaaac
    16681 aattgaccaa tgccagcctg aacctggtcg aagaggccgg acacaggttc cttgataaga
    16741 cagctgtacg agaagatatg acgctggcgt ggttggaaga attgttttta caataggttt
    16801 ggaggagaac aggcaattgg atttttccag cattgaatcg acggtgcatt atataaaaag
    16861 ttttggtgtc tatgggccgg cagcagcttt catattgttc tttgtccagg ccgttgcgcc
    16921 aattgttcca tatattatta tcgccggtgc agcgggaatg atattcggta aatggattgg
    16981 ttttttgctg gcctggattg gtgccattgc aggggcctgg tttctgtatt ggatttctga
    17041 caagattgcc ggcaacctcc tggtcgtcaa gctgcagcat cgttatgccc tggatctgac
    17101 ccgcatcgat catcgccata tcttttgggt attgctggcc tgtcgtatat ttccggtagt
    17161 gccgacaccg atcatcaaca tcggttcagc cttggcggga gtaagcccga aaatattttt
    17221 gtcatcatca gccatcggca aaatgccttg ggcttttgtt tatgttgctc tgggcaacta
    17281 tctgatgcaa accaaaaaca tcagcaacac cctcaccgtg atcgggctta tcctgctcat
    17341 atcatttatt ggcattacct atttacgcaa actgcttccc aaatattatc gtgaaaaaca
    17401 atagcgaaat tgattgaata atataaaaat aaaataaata ttaaaaaata tgtcaattta
    17461 gcatggccca aattaaatgg gccatgttta gtttgttcag aaatatcgga ggtttcagaa
    17521 taatgcaaat gcttttattt gcatatttaa gaaggtgatt taataacaat gtagaatata
    17581 ttaataaaaa aagtattacg aaataatatt gaggaggagt atggaatgtc tgctaacaat
    17641 tttattctcg acaccagaga ccataagttc gtactgaaag aatggctgga tgttgacaaa
    17701 gttttaagct ttgatgctta caaggattat tacaccaaag aagatgctga tactttcatt
    17761 gaactggtct ataaaatgtg caaggatgtc atcgcaccgg ccaacggaga atgcgatgag
    17821 atcggcaaca aattcgttga tggcaaggtg attacccccg aagccagcaa aaaagcgtac
    17881 cataccattg ttgatgccgg cttggggccg cagtttggcg atcgcgaagc ggaaggccgt
    17941 atgcctatgt atttaggatc agcactttgc gagatggtaa ttaatgcttc gccggccatc
    18001 tccactctgt atggattgtc gatgggtaac ggggcagtta tcgccaattt tgccacccct
    18061 gaattaaaag aaagattcct gcccaaaatg ttcgcaggtg aatggggcgg aaccatgtgt
    18121 attaccgaag ccggagccgg atctgatgtt ggcaacgctt ccaccaaggc ttatccgaca
    18181 gataccccgg ggttgtacaa aattaaaggt ggcaagatgt ttatcactgc cggcgaccat
    18241 gatatctgcg aaaacttcat ccatctggta ttggcgcggg tagaaggtgc acgcgaagga
    18301 accggcggcc tgtcattgtt tatcgtcccg aagatttggg ttaatgaaga tggcagtctg
    18361 ggagctccca atgacgtcaa tacggtcgga attgaacata aaatgggcat gaaaggttct
    18421 ccaacctgta cgctggcctt tggtgaagac aacaactgtt atggcttcct gattggcaat
    18481 ccgcctgacg aaaaaggcaa gggcgaaggc atggcccaga tgttccaaat gatgaacgaa
    18541 gaaagactgg gaacggctgt tttggctctg ggaactgcat ctgctgctta ccaacagtca
    18601 ctggcctatt ccaaggaaag agttcaatct gccaagctga ccgatatgaa aggcggcggg
    18661 gtaagaatta ttgagcatga agatgtcaga cgcatgctga tgttccagaa gtctattatt
    18721 gaggcctgcc gggcgatgat tatgaagact tattattata ttgatctgat gcatgacagc
    18781 cctgatccag aagaaagaga atttgctgat gccatgttcc atatgagtaa tccgctctgt
    18841 aaggcgtata cttcagatat ggcatggcag accatttgcg aagcgattca ggtttatggt
    18901 ggttacggct ttattgaaga atacccttgc gcccaatatg cccgtgacgt gaagatcttc
    18961 tctatttggg aaggcaccaa ctatattcaa gcgctggatc tttgtgcccg caagatgacg
    19021 atgaaaaaag gcaaagtcat gatgaccttc atgaaggaaa tcggcaaatt cctggccatc
    19081 aataaggatg cagcaggttt tgcagatgaa atcaagatct taaatgaagc tttcactgat
    19141 taccagggga tcctgggttt gtttaacggt tttatgactt ccggcaaaat taacatgctg
    19201 ggcgtttttg caacccgtat cctgcacgct acagccatgg tctatgccgg gacattgatt
    19261 cttgatcagg ccattctggc gaccaaaaca ctggcagaaa tcggtgacga tcactttgat
    19321 gccaccttct acaagggcaa aattgccagc gcccgtttct ttatcaagaa cattgtgccg
    19381 caggtattca atattaagag agtcatggaa attggcgaca gtacctgcgt tgatattccc
    19441 gaagactgcc tccgctaata cagatccaag ccttggacca attattgctt aaccgagccg
    19501 cagacagctt tacagcttag tctgcggctt tctttgtctt gctggaagag cggaagcatt
    19561 gatgcaagaa gatttccagc ccgtacaaaa cgaactcctt ttcagaagtc tacatgtaaa
    19621 aggacaatcg tttcggtaaa atatgaatct tttgcggtaa tttgcttttg agaaagaaat
    19681 ctctgtgtta aaataattat aaatatttct gcatttccca atatacttta atatccaata
    19741 attgagaatt aacagtgaat aaatgggcgg gcagagtgat acaaaattga ttgcgagggg
    19801 gtatattgca gcgttaaata ccacgactca gagaatttcg ctgataattg cgtaatggat
    19861 attacggaaa tgcatcacgg taaagaggaa aagaataatt tggcgaatat gaggaggcgt
    19921 tttacgaatg agtgctggca attacattta tgacatgcgg gatcttcagt ttattcttaa
    19981 agaatggctg gatatggaca aactgctcgg atttgaagcc taccgggatt attactccaa
    20041 agacgacctg gattccatcc tggatgtaaa ctacaagatc agccgcgatg tcattgctcc
    20101 tgccaacgtt gaatgtgatg agattggggt aaaatttatc gacggccagg tcatcacccc
    20161 ggaagcgata aaaaaagcct acgcagtcgt tatggaagct ggactgggac cgcagaatgc
    20221 agaccgcgaa gtggaaggcc gcatgccttt ggtaatgaac gccgcgatcg gtgaaatgca
    20281 gatcaatgct tctccggccg taaccactta ttgggggttg accatggggg ctggcggagt
    20341 gatccagtct ttttccacgc cagaattgaa ggaaagattc ctgcccaaaa tgttcaccgg
    20401 tgaatggggc ggaagtatgt gtattactga agccggtgcc ggatcagatg tcggcaatgc
    20461 ttccaccaaa gctttcccga ctgatactcc cggtttatac aagatcaaag gcggcaagat
    20521 gtttattaca gccggtgaca atgatattgc caagaacatt gtccatttgt gtctggctcg
    20581 tatcgaaggg gcacgcagag gcaccgccgg actgtccctc tttatcgtgc ctaaaatttg
    20641 ggtcaacgaa gatggttcaa tgggagaaag caacgatgtg accaccgttg gcatcgagca
    20701 taaaatgggt ctgaaaggtt ctgccacctg caccctggcc tttggcgaaa acgacaagtg
    20761 ctttggctgg ctgatcggca atccgcccgg tgaagacggc aaaggcgaag gcatggcgca
    20821 aatgttccag atgatgaacg aagaaagact ggctaccggc ctgctgggct tgggcgtttc
    20881 atctgctgcc tatcagcaat ctttggctta cgccaaggaa aggacccagt cggccaaatt
    20941 gttggatatg aaaggaccgg ccgtcagaat tattgagcac gaagatgtca gacgtatgct
    21001 gatgctgcag aaagcatata cagaagcctg ccgggcgatg atcatgaaaa cctattatta
    21061 tattgatctg tcccatgaca gtccggatcc ggaagataaa gaatttgcca acctgatgtt
    21121 ccacatgacc aatccgctgt gcaaggctta tgcaactgat gtcgcctggg attccattgc
    21181 tgctgccatc caggtttacg gcggttacgg gtttatcgaa gaatatccag tcgcccaata
    21241 tgcccgcgat gtaaaaatct tttccatctg ggaaggcacc aactatatcc aggcactcga
    21301 cctctgcgct cgtaagttta ccatgaaaaa aggcaaagtt ttgatgactt tcttcggcga
    21361 gttgggtaaa ttcctggctg ccaacaaaga agcggccggt tttgaagaag ccatgcaagt
    21421 attcgctgat gccttcactg cttatcaggg aatcctgggt accttcaaca attatatgac
    21481 ctctggcaag atccagatgc tgggtctcta tgccacccgt attctccacg catccgccat
    21541 gctgaactgc gccatgctga ttcttgatca ggccatcctg gcttccaaga aattagccga
    21601 aatcggtccg gatcattatg atgccagtta ctataccggt aagattgcca gtgccaaatt
    21661 ctatgtcatg aatattctac cggatgtttt caccatcaag aaagttttcg aaatgggtga
    21721 cacatctgca gttgatattc cggaggaagc gctgcgctag gcaatacgga aataaataaa
    21781 gagaagacag cgggtctgtt gccgacagat ccgctgtctt tttgccaatg atgctttcag
    21841 tcgataagtg aagaaaaatt aatttgtgaa cataatagtt atccggttga aaagacacga
    21901 aacaaaataa aaaaaacaaa attttgagag gctgcccgac cgtcatagaa ttaaagtgtg
    21961 aaaaaatgag gattacaagg tgttacgata attagatact taaaattaga ataaatttac
    22021 atatatattc tatagcagga actaaaattc ttgcgttagt tattccgaca aagtaataaa
    22081 cttctcctta aattttcttt tttgctaata ttgctctatg ttaaaataat gtttaatatt
    22141 tggaacattt cctaaaattc cagctattca aattattttt acattcgatg cagggaaggc
    22201 aacaatcaat gtttgcaaat aaacaagagg gggagtgact taatattatc gctgctgatc
    22261 atttttgcag agaaagctgg tcacgggaga gtcgcttgta ctgcaaaggt tttcatgtca
    22321 gcccggggcg tagatttgtt ttttaagaga ttttaaggag gcggattgga ttgagcgcag
    22381 ggaattttat ctatgatacc agagatcagc tgtttatttt gaaagaatgg ctcgatatgg
    22441 acaaactgtt tggcatggaa gcatatcgtg attattacag caaagacgat attgattcct
    22501 ttatcgatgt cagttataag atttgccgtg atgtgatcgc tccagccaac caggagtgtg
    22561 acgaaatcgg attgaagttt gtagatgggg tggtcgttac gcccccagcc gttaaaaaag
    22621 cttatagcac ggtgatggat gcgggtttgg gaccgcagag cgccgaccgt gaagcggaag
    22681 gacgtttgcc cttggtcgta tgtgcggctt atggcgaaat gcagatcaac gcatctccgg
    22741 cagtggctac atactatggc ttgacggctg gtgccggcgg cgttattcaa acctattcaa
    22801 gccctgagct aaaagaaaga ttcctgccga aaatgttctc cggtgaatgg ggcggaacca
    22861 tgtgcatcac cgaggccggt gccggatctg atgtcggcaa tgctgcaacc aaggcttttg
    22921 caacggatac ccccggtctt tataaaatca aaggcagcaa aatgtttatc accgctggtg
    22981 atcatgatat tgccaaaaac tttgtccatc tggtactggc caggattgat ggtgcccgca
    23041 aaggcacggg cggactttct ctatttatcg tgcccaagat atgggtcaac gaagatggct
    23101 caatgggtga cagcaatgac gttcttaccg ttgccattga acacaaaatg ggtctgaaag
    23161 gttcagcgac ctgtcaactg gcctttggtg aaaacgacaa atgctttggc tggctgatcg
    23221 gcaatcctcc cggagaagac ggcaagggtg aaggcatgtc acagatgttc cagatgatga
    23281 acgaagaaag actgggaacc ggtatgctgg cattaggtac tgccaatgct gcttatcagc
    23341 aggccattgc ttataccaaa gagagagttc agtccgccaa attaactgat atgaagggcg
    23401 gcgctgtcag aattattgaa cacgaagaca tcagacgcat gctgatcaag caaaaagcct
    23461 gtgttgaagc gattcgtgcc atgatcatga agacttatta ttatatcgat ctttcccatg
    23521 acagcgatga tccggaagag agggaattcg gaaacctgat gttccatatc ggcaatccgc
    23581 tttgcaaggc ctatgcttct gacatggcct gggatttgat ctgcgatgcc attcagtgct
    23641 acggcggtta cgggtttatc gaagaatatc cggtcgccca gtatgcccgc gatgtaaaaa
    23701 tcttctccat ctgggaaggc accaactaca tccaggcgct tgatctctgt gcccgcaaat
    23761 tcaccatgaa aaaaggcaaa gtgctgatga gctttttcgg tgaagttggc aaattcattg
    23821 cagccgccaa ggaagctgcc ggttttgaag atctgattaa actctacgaa gaagcctttg
    23881 cggcctttgg gggcatcatg ggttccttca acggctatat gcaaggtggc aagattccga
    23941 tgctgggtct ttatgctacc cggattctgc atgccacctc catgatatat tgcggcatgt
    24001 gcatccttga tcaggcgatt ctggccgcca agaaactggc agaggttggt gaagatcatt
    24061 atgatgccgg tttctacaaa ggtaaaatca ccagtgccag attctatatc atgaatattc
    24121 tgccggatat cttcacgatt caaaaagtca ttgccatggg agataccagc gccattgata
    24181 ttgctgaaga aaatattcgc taaccactac cgggaatcat tgaaaacata ttaccgcagg
    24241 tctggccttg agctggatct gcggtttttt gtcacagcaa aggcaggaag caaaaccaaa
    24301 aaacggacgc tatgatggca ttgccaaagg cgataaagtt gtttatgaag ctaatcataa
    24361 atagggcagg aaaagtggtt gctgtttctt gctattttaa aatttacctg ttatgatgct
    24421 tttgaagtat aatatttatg cgtactggaa aagaaaggag caggtttatg cccgacaaca
    24481 aagaaagatt actgattgaa gtcctgcagg ggacccagat gatgagcggt ggatgaaact
    24541 gcagcagcga ctgctcgtca gcagccagtt gtggaacagc tgtcagttac gaagaaacgg
    24601 ttaatatcat ggccaatgaa ctgaaacttc aattcggcga tcgggttgaa gtaaaatatg
    24661 tcgatgtcga taaagaggga ttggataatt atccgatcat gagccgggta ttgcaaatgg
    24721 gttatccgta tccgattacc ttgatcaacg gagaaccaaa gtttgccggc ggcattatga
    24781 ctgccgaagt agtgcaaagt attgaggaat tattgaaaac ctcgtaacca taaaccgagt
    24841 gacgaaaacc cgttctgtag aaatgaacgg gtttttaata tttttaccta taattgctca
    24901 tcataaaata attccgttta ttcagaacgc attactgcaa ttttaaggca ttctaataac
    24961 atgccagcct cctatattcc aataaaaaaa taaactgccc ccttaattca ccaatcgttg
    25021 actattgtct atgataaaat agtatagaat atttacatat ttccaaaaat tatatcaagt
    25081 ttagctgaaa tcttttgggg gaacggtcga caagttatta aaaaacaggt gagggggagt
    25141 agaatgtatc agggagaata ccgtcgcaaa ctggtattag ccgaggaagc cgttaaatgt
    25201 gttaaaaccg gtgatattgt tgattatggt ttctttaacg ggaaaccagt gctttgtgat
    25261 caggcactgg cgaaacggac cgaactgcgc gatgtcaaag tttattgtgc ggtcaccatt
    25321 ccaccggttc cagaggttgc caaagtaagg gattgtttta cctaccatga ctggcaattc
    25381 agtaaaatcg cgagagtcct gcagagttac ggactgtgtt attattcgcc catactctat
    25441 catcaggcac ccagtttgta tcgagatggg atcgctgaac acagaaatgt tgccatcatc
    25501 agggtctgcc cgatggacaa acacggctgg ttcaatctcg gaccgcaaaa ttcagaaacg
    25561 atggcaaatg cagagtcagc tgattatgtg atagtggaag tcaataaaag tattccggta
    25621 tgtttgggag gcgctgagga agccatccat atatcccagg tggattacat tgtggaagct
    25681 tctgatgatg acattctgcc ggatatcccc ggcgctgttc ccagcgaaga agacaagcaa
    25741 attgcccgcc acattatgga ttatgtccac gacggatcat gcatccagct tgggattggc
    25801 ggtatgccca atgcggtcgg caagatgatt gctgactccg atctgaaaaa cctgggaggc
    25861 cattcagaaa tggtggtcga tgcctacatg gatatgatgc aatccggacg tatgaatggc
    25921 cacgccaaac agattgaccg gaacagggta gcatatacct ttgccattgg cagtcacaaa
    25981 ctttatgaat acatggacaa taatcctgcc tttgcctcct atccggttaa ttataccaat
    26041 gatccgcgga ttatttccca actcgacaat tttgtatcca ttaacaattg cgtcgagatc
    26101 gacctttata ctcaggtaaa tgcagagagc aaaggtccgg tgcaaatctc cggtaatggc
    26161 ggaatgtggg attttgtcct gggggcacag ttctcccggg acgggaaaag cttcctttgc
    26221 atgacttcat cttataagga caaagatgga aatttacact cacggattgt gccaaccttt
    26281 actccggcca gcattgtaac cattccacgt cagcaggtgg attatattgt gaccgagtac
    26341 ggtgcggttc agatgcgcgc caaaccaacc tggcagcggg cggaaaaatt gatcgagctg
    26401 gcccaccccc agttccgcga tgatttaatc aaggaagcag aaaaactcaa catctggagg
    26461 aagattaaca aagaagacgt gtaaatcatc ccggacatgg ctgaccaaga attgcaaaag
    26521 gctgttccaa atctgaaaac agataaagga acagcctttt cgtttatggt tcgcaaccac
    26581 ttttttgccg aatgtttgat caggaaattt cacgtttatc atcatctttt accgaaccgc
    26641 ggaacccttt gatcgctttt cccatcgatt cagcaacttt gggaagcttg ccggggccga
    26701 caatcattag aataatcaac agaatgagcc ccacttccca gggaccgaaa ttaccaatca
    26761 gagcaaacat cagagcgcct cctttctctg gttaatttgg agaagccgaa taaacgatca
    26821 ttgtctatgc ataactgtgc agacctggca atagataatt gaccccaaag aaggtaaaca
    26881 gcaccagtac aaatccggca atcagcagga tgcgggcttt attgccccgc cagccgcgct
    26941 gacggtgcag gtgcagataa atggcgtaaa tgatccaggt gatcaatgcc caggtttctt
    27001 tgggatccca ggtccagtaa ctgccccata cttgttcggc ccagattgca cccaaaacaa
    27061 ttgacaaggt aagcatgaca aatcccaacg aagtaatgcg gtagatgtca tgatcgtccg
    27121 caggattgtt ggcttgcaat tttaccagtg ccagtcccga gacgagagca aaagcgctgt
    27181 aggcagcaat ggcagtcaaa acatgggaga tcagccaggg gcttttcaat gcaggcatca
    27241 agggaccggc ttgcccgatt tgctgaggca tcaaaaccat aatcgatacc atcagcagga
    27301 cattaatcaa cataaccgca gctcccgcag cggcatgccg gctccgtttt tcatagagca
    27361 gatgggataa tacggtaatg aaaacgaaac tcatcataaa ttcatagcca ttggttagcg
    27421 gcaggcggtg actataggca acatgataag ccagcgccag gaagttgcac agtaacccga
    27481 acattgccag ccaggaagca tagcgcatcc aggcaggggt ttgacgagct gcccaataaa
    27541 ataccatgga ggccagataa agaagaaaaa tgacatagat aagcagatgt tcgattgaga
    27601 tcatgatgcg atatcctcct tgtctgactg tgagcggtta ggttttttgc ggggcatgag
    27661 caaaacgata ctgataccca gcattagcaa tatgcctccg gcagctgtca gcggcatccc
    27721 agggtcggtt tttaccgtca ggatcgagta aggtcttacc tgggcaaaag tgatgtacgt
    27781 ctgctcgtct attttgatcc gttccttgaa aggcgcggtc cccaccccca gcatatgctc
    27841 gccttcataa accgtataaa tgacgcgcgg atttttcggt tccagggact tggtctgcat
    27901 gcccagagat ggatcaaaat gagggatata tttaaaaacc ttgacagtgc gacgggtcga
    27961 ttcaaaactt atccagtcac catcctgtac cagcagatct ttgcttgttt ccccatcttc
    28021 ggcgccagcg ttcaacaaat agccataact gttttgataa aacttgaccc cggcaaaggt
    28081 gaggggatga ttcacactga tggtctgttg caattcattc tgaccggaag aagcaacctg
    28141 cagggtagag taatactgcg ccggagaacc atcttcatta aaggggatgg aaaaatccaa
    28201 cagttttact tgaaaaggag tatttaccgg gataatccgg ttgatctcca ccgtatcgcc
    28261 ctgcagaatg ctcaattgca ccgaatgccc ccaccaggaa tttagactgg caccaacaat
    28321 aatcaaaaca ataccggaat ggagcataat aaggcagatg cttcgcaaca tctgcctttt
    28381 gttgtgcagg attctctttt tgccggcaac caacttttta atgctgttga aggtacacaa
    28441 agccatgttc acaaagaaca acagcaacaa cagccgaaaa taaacagttt taaaaaacat
    28501 ctcgggccac aggcttgagc caatagccga agcgatgcca atcaaaccca acaggatcag
    28561 acctgcgttc atcgaagaaa tataattgta aaagcgcatt cggtcatctc ccgggcatca
    28621 atgattaaat ttcttcttca aattcggttg accagctttc gtccaattca tcataccatc
    28681 cccaaagatg acattcaaca cacatcggtt tggacttgcc atgcatgttg tgacacagat
    28741 tacagtcttg ttcaccgtga tgggaatcat gtggattgga gtcttcataa tctgtggctg
    28801 ccacgatttc atcccagcct tctccttgac cgccttcctt gtggcaatcg agacagaaag
    28861 cgcggtcgcc gagagcggcc tgcagcggaa gttcatagtt gccggtgata taattgagac
    28921 cttccatggc tttttccgga atggtacggt gatggcaatc caagcattca atattttcca
    28981 gggcgtgttt gtgatcgagg taaattcctt cattccagga ttcataaaaa ggcctgataa
    29041 tatggcaggt tttacagaaa gaaggatttt cactggcctt taataatccc gctgcgacca
    29101 tcacgcccaa gaccacagca atgctcagag cgataaggat taccttcttg tttagatatt
    29161 tttcccggaa acttttgttt tcatgatggt tgtctgtcat atacttcact cccgtttaca
    29221 tcttaagtct ctaaaattca actaattgtg taaatattct aaataatatg ataaaatttt
    29281 aacataaagt tttctttaat tgtagcggca aaagtaagtt ttgtctttgt gatattttac
    29341 agtaattcct gcttgctttt ggtgacagat tcttattctt tagcagaaat aaaaaggctt
    29401 gttagattat cacacagtga tttgggcaaa tgttcgtgaa tgcttctgat gaaattatcc
    29461 gttt
//
LOCUS       DAOTHZ010000022        29069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1815_length_29069_cov_6.989316, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000022 DAOTHZ010000000
VERSION     DAOTHZ010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 29069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29069
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1815_length_29069_cov_6.989316"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /gene="dtd"
                     /locus_tag="P5549_04205"
     CDS             complement(<1..417)
                     /gene="dtd"
                     /locus_tag="P5549_04205"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRW12304.1"
                     /translation="MRAVIQKCLHSSVVVDNRETARIGPGLLVLLAVKKGDDCQDADY
                     MMDKIMNLRIFEDEQGKMNLSLLDTGGEMLMVSQFTLYGDARKGRRPSFSEAEDPEAA
                     KALLDYCEERIRVKGIRLATGTFAAHMQVNLCNDGPC"
     gene            complement(418..2577)
                     /locus_tag="P5549_04210"
     CDS             complement(418..2577)
                     /locus_tag="P5549_04210"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRW12305.1"
                     /translation="MDRIKQYDLNANFDLITKAYSYAEAAHGGQKRISGEPYIIHPVE
                     VAMILTEIEMDTASICAALLHDVIEDTAYSFLDLQEEFGEEIALLVEGVTKLSRIEFS
                     SREEAQAENLRKMFIAMAKDIRVIMIKLADRLHNMRTLQYQPEHKQKEISRETMDIFA
                     PLAHRLGIFKFKWELEDLAFLYLDSEMYYEIARRLKTKRKDREAYVHELIQRIAKGLD
                     QIGIQGDIAGRPKNIYSIYKKMLKQNKGIDEIYDKIAIRVIVEHVRDCYGVLGIIHTM
                     WKPLPGRFKDYIATPKPNMYQSLHTTLIGQGGEPFEVQIKTWEMHRTAESGIAAHWKY
                     KEGITGEKDKKFDEKLAWLRQIMEWQQDIKDTGEFMESLKIDLFEDTVFVFTPKGAVF
                     ELPKGSCPVDFAYRVHTEVGHKCIGAKVNGRIVPLDFQLANGDIVEILTSKQSNGPTA
                     DWLNFVKTSSARGRIKHWFKREKREENILKGHDTLELELKKKHLDPRELMKECKMLEV
                     GARFSLNKADDLYAALGDGSITVNQVINKMKEMFFENIQLDDVLNVPQTTGVHRDRET
                     SSLKIKGVDDVVIRLAHCCNPLPGDEVLGYITRGRGVSVHRRDCPNLINYLRNEKDRV
                     MEVEWASEIGVYVVELEVKAMDRPRLTTDVMNVIADTRIQINSVFSRVTKNGLALLNL
                     KLEVMDMGHLTAVKQRIQKVKDVIEVRRVLPGEIRGE"
     gene            complement(2596..5433)
                     /gene="recJ"
                     /locus_tag="P5549_04215"
     CDS             complement(2596..5433)
                     /gene="recJ"
                     /locus_tag="P5549_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRW12306.1"
                     /translation="MHSIWQLKPYNDEIASTISRELNINLPVARLLVQRGNQNAAQAS
                     RFLYPQLNMLEDPLKMKGMKQALERIDKAMAAGEKIVVYGDYDADGVCSIVILMECFE
                     RMGYPVDYYVPNRFREGYGLNAEALQLLSERGCNLVITVDCGISSLAEAELARSLGMD
                     LIITDHHTPAEKQPAAHAVINPKNDQLPSIANLAGVGVSYKLACALMRAKGMEPGQDW
                     LELVALATVADIVPLRDENRILVKYGLVALGNTTRPGLIALLEKTGLCGKPITAVHIG
                     FILAPRLNSAGRIDSARQSVALLLSKNPEEARLLAEELCRLNDERREIEESIYEQALL
                     ALPDLEAQRFILANGENWHEGVIGIVASRLANKFHRPAIVISWEGEQGKGSARSSGDF
                     DLHAALQACSSHLQRFGGHRMAAGLGLRRDKLTSFCQALQEYMLGQELPALGKKIYPV
                     DLEVDEADLSLELWNEIEQLAPFGEGNPSPKLVLRSSPLHELWLVGANEAHFKFKTGD
                     KHLDAIAFNGADMMKPALKICKQDLLFDLAENNFKGRSSLQLKIRDIKPAWRDDQGVG
                     DNAYSEQLKTVVRTTIDELVNGHPVLFIYPTLRSLKKHLVMLQALVKAGLLVEMHGQL
                     EKKSREIIMRQFRQGESRIYLSTQAFMEYLAAPGESGDLHLPGWPSSLKTAVRFWPET
                     EEMVAGWQQPDLHRLAVKLNPRLHFQISQDQREGTGRHVIYVNRPASVNTVLQQHPRA
                     AVEAGNIAPDLRRSIRQRFRNNRTGLLLVDGTHPPGAGQLGRIDAFTLLDSPFACCEL
                     AAVADYVDNLELPLQVGFTAADLDRNDSYLQRIYPEPSRLGLIWHTLAAAGSGKALRL
                     EEAALLTRLNRDVGSEISRLELSAALRIMTDLDLCQCQKSGSIMAINFHSTAKDALRP
                     ESSPYFQEGKKEKEQWERWRTMVQKYTGMVM"
     gene            5669..6304
                     /locus_tag="P5549_04220"
     CDS             5669..6304
                     /locus_tag="P5549_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12307.1"
                     /translation="MAGTRHLFPGANSCYGFFSFYDQIVPPQVKTKVILKGGPGTGKS
                     TFMKSIADDLGSQQIEAECHWCSSDNNSLDAVVGGHGQVCILDGTSPHVVDPRYPGAV
                     DRIINLGEYWDASLLKKNRADIEKLTDLIGLSFQRATTVWQNPNWPSGNGNPTFRKPG
                     TSKQSNATFWRWRKNFYRIIERPGAGCVICLPAPSLLPGLLPRLRVCWKAI"
     gene            6289..6765
                     /locus_tag="P5549_04225"
     CDS             6289..6765
                     /locus_tag="P5549_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12308.1"
                     /translation="MESDLTVLGVKGSPGGGGKELMRYILTRIELGGVFAEVFHCPFD
                     PEEIDMIIIPDNKTALVDFSSAVIDYETAWPTRRFKRFLDFDQFLDQSVLDSRAKAIF
                     SSRERFAAGIRAAVAHIKTAKEWHDELESFYVPAMDFERIEEVRQELLTELLAELQ"
     assembly_gap    6922..7021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7184..8392)
                     /locus_tag="P5549_04230"
     CDS             complement(7184..8392)
                     /locus_tag="P5549_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRW12309.1"
                     /translation="MQEFLKVLTYQNAREILREHFSLPSTEMIALQDCFDRILADDII
                     SPENMPAFTRSTVDGYAFNCTDSYGSSESLPGMLAYIGSVSMGKETPLKVGPGQCAWM
                     PTGGMLPEGSNAVIMVEYTERLDEDTVLVFRPVGAWENVMQSGEDIAAGDLIFSRGHK
                     VRPQDIGLLASLGIKEVSVYSPYHVGILSTGDEIIPFQEQPRPGQIRDVNTWAISAAL
                     QSCGAVACPYPLVKDDLASLKQAIAFGLKENDLLLVSGGSSIGIADYTLEAMLSMPDS
                     QLLFHGIAIKPGKPTLAVKTGSQLIIGLPGHPVSALMIFHIICAPLLRQYKAWQEKGR
                     LTVNVASQAGRDDFIPAQLINCETGLAVKPLLGKSGLMNILAQADGYIHIPYEKQGLP
                     ASEEVPVTLF"
     gene            8496..10412
                     /locus_tag="P5549_04235"
     CDS             8496..10412
                     /locus_tag="P5549_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HRW12310.1"
                     /translation="MSRNIYIDNMPQEKALQLFMDKLQSCGWFQRTVEYSPVRESLYR
                     VTAEPVIARRSSPHYVASAMDGVAVKSAMTVGATEASPVNIMAGDYLEVDTGDLVPKE
                     YDAVIMIEEVNFFDDHIQIIKAAVPWQHIRSIGEDMVEGDMVLSSLRAIGPYELAALI
                     TAGVETVPVIKKPVLAVIPTGTELVDTAGITMAPGDIVESNSYMLMGLAREWGAECLR
                     HDIVIDDREQIRQAVLEVEPLADIIVICSGSSAGREDYTAGIVRELGEILVHGVAVRP
                     GKPAILGIIEQKPVIGVPGYPISAGLIFNLFARPILYRKAGRHLPGDKTMTATISKKL
                     PSPMGVDEYINVNAAVINDRYIAYPLNRGAGLSSILVKSDGTVHVERGQEGLQAGAGC
                     KISLHRSRREMQNSLITLGSHDMSIDFLIDILYRDHGIRLVSTNVGSMGGIMALRRKE
                     THFAGLHLLDEEGGYNISYLHKYLPNEKYLLVNLVKRHQGLVVAPGNPLGIKSISDLT
                     RSDLRYINRQKGAGTRILLDHLLKQKGISSADINGYQREEYTHLAVAAAIKNDACDTG
                     MAIFASAKAMGLDFVPIDVERYDLCILTETMPDQQLECLLSVIRSDEFKERVLFFGGY
                     ETDLTGQIIYQQGY"
     regulatory      10521..10641
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10886..14086
                     /gene="fdnG"
                     /locus_tag="P5549_04240"
     CDS             10886..14086
                     /gene="fdnG"
                     /locus_tag="P5549_04240"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:11513..11515,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HRW12311.1"
                     /translation="MKVTRRQFLKVTGATAAAMAVTDLGFNMNATASAIKELRIKNVT
                     PTPTVCPYCASGCGLVVYAQKDSAGKFQKLLSVHGDPDNPINIGGACSKGAAMFNLRE
                     IYDPQTGEQIINPKRVQKPLYRAPGTDKWVEKDWDWMLDTIAERIKETRDSSFIHTEK
                     LADGNDVIVNRTEKIASLGGSGLDNEECYLLSKMMRSLGLVYLETQARIUHSATVAGL
                     SPSFGRGVMTNHVVDLKNTDCALIMGSNAAENHPISMKWLIRARENRGAKIIHVDPRY
                     TRTSARADLYAPLRSGTDIAFLGGMIKYIIDNELYHKDYVVNYTNAAYLVKDEYGFED
                     GLFSGYDQENRKYDVSSWDYKYGEDGLPLTDPTLQDPRCVFQLMKKHYERYDIDSVCK
                     ITGTPKNKYLEVLDMFCATGAPNKTGTIMYAMGITQHTVGSQNVRSFAVVQLLLGNMG
                     RPGGGVNALRGENNVQGATDMALLFHIIPGYIDSPMNNAAHKDLISFVRSVTPGAAKL
                     PFTNLEELRELVKEGFPNSGWKIHSSKWVVSLLKAWYGEAANAGNDYAYHYLPKRDEK
                     KNYSHMQLFEAMHRGEIDGLFVNGTNPVVGGPNANKEHEALTKLKWMVSIDLWLNETA
                     DYWTHQAWDRPVENPNVAKYTPAEIGTEVFFLPAAAVYEKEGTAAQTGRWVQFRWKGA
                     DPVGESKSDLWIYNELGKRIKKIYAGSTRKEDEPIVNMTWDYDNEHGEDDIMKVALEL
                     CGFNVADGKPVEGFAKLKDDGSTACGNWVYCGAMTFKDGKTIYKPQLRKKDDPSGLGL
                     YPEWGWAWPMNRRIVYNRCSVQPDGVTPWPGDEERQLVRWDPNAIADPTKPEVLGKWV
                     GVDVPDFIGTTGPDKPAFTKPFIMRPEGHGCLFAAKTSMKDGPFPEHYEPWESPTANK
                     MNSHNLNPATKVIEPDKQGDAEKYPIIGTTYRVSEHWQTGALTRNLPWLAELMPNMMV
                     ELSEELANEKGIKNGHDVIISTIRGDVKAVACVTKRFKPLVIHGKHVHEIGLLWQYGF
                     KGFATGDIANWLTPHIGDANTMIPEYKAWLCDIRRA"
     gene            14089..14898
                     /locus_tag="P5549_04245"
     CDS             14089..14898
                     /locus_tag="P5549_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW12312.1"
                     /translation="MANLTNLYDVSKCTACRGCQIACKDWNQLPASIEPFKGNYQTHE
                     DTDGDTFTIIKFFETEDPVTGVNWSFIKHQCMHCFEPACMMACPKGAYTKTETGATVH
                     DPDKCIGCQYCTYACPFEIPKYRKREDKISKCTLCVDRTVEGLTPACARTCPTGALTF
                     GDRDELLQKAEDRAKYLRENGYPNATIYGKLELGGLNKLYVLTDTPDKFGLPVDPHIP
                     KNISAWQNVVQPYFGWLIPLMLGASAVSFVTTRLLANKHGGHGEHEEGGHE"
     gene            14898..15581
                     /locus_tag="P5549_04250"
     CDS             14898..15581
                     /locus_tag="P5549_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRW12313.1"
                     /translation="MQWLTEYKPDVERVERFNFVARLTHWGHTVTFLLCLFTGLVLFL
                     DGVDWLAAIFGGYGGAGLVHRIAAVLLTIFLAVFVVFDFKGIINWIKDILRFGMNDIK
                     FVMKFPLEFFGFPVKVPPQTRFNGGEKGNSIVTPTMVILLVISGYIMWFPAIFPAGLV
                     RVAYPTHDIAMILATCMVCMHAYLGSFHPGSGESFWGMWKGTVRSEWAKHHHAIWYDE
                     TYGDKTDAE"
     gene            15750..16580
                     /locus_tag="P5549_04255"
     CDS             15750..16580
                     /locus_tag="P5549_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HRW12314.1"
                     /translation="MNRQTPVALPEGYVEFYSNLENWQNEQEIVLKKNHSPEHIEVRK
                     LLANSQKHLMKQHRFTIDPQQLRETYLALVVFLQSNRPITVEMIEILRKAADQLDFDN
                     LAAILLDNDPTYFIEMAEDLGIASELLVFTVDHALRPYLRMLAQPYHHEFSQEEIGFW
                     SQAAFCPFCGSKSHFSRMREADGKRLMFCDRCFTEWESRSIYCVHCGNDEPNTINYIS
                     VEDDPAYQIYTCDKCKGYLKTYDERTTGKTTDLFIANIETIYLDLLAQEKGYVNHDAD
                     "
     gene            complement(16665..17963)
                     /locus_tag="P5549_04260"
     CDS             complement(16665..17963)
                     /locus_tag="P5549_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRW12315.1"
                     /translation="MELKYQIEDKPVWKENGLYGLQWLAVSLPAVITFGKVLGGMDSP
                     ALEILYLQKLFAVMGVALLIQVFWGHRLPVVLGPSTILFIGILASLESTPAAIYTSLL
                     ICGALLALLAVTGLFGRLQSLFTPRVVAVILLLVAFTILPTITNLVIGGTEVPGAGRR
                     LIFAFVFMLLMFVGGRYLQGLLKNTLVLVAMVLGSLAYFLIDPGWAAHASVENIQPVA
                     LFFSHLNTSLVIEPGVLVAFILCFLGLSINDLGSIQAVGLVLKAEAMPRRITRGIAAT
                     GIGNVLAGWMGVIGPVNFSFSPGLIAATGCAARRSLISAGVAMLALAFLPRIIFYLSF
                     IPAAVIGCVLFYTMCTQVAAGLMTAFDALQELGEPRFDNALMISLPILMGIIISFLPA
                     EITASYPYSLRPILSNGFVVGIVTSLVLEHTIGRPLKQKK"
     gene            complement(18057..18401)
                     /locus_tag="P5549_04265"
     CDS             complement(18057..18401)
                     /locus_tag="P5549_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRW12316.1"
                     /translation="MDKQNRKELRAAYKEQKDIGGICAIKNTANGKTLILPVNNLRAY
                     QSRFAFSQQTGSCVEEKLLSEWEKYGAQSFTLEVLEELEKMEEQTPKEFRDDLKTLKD
                     IWLERFEAKQLY"
     gene            complement(18405..18884)
                     /locus_tag="P5549_04270"
     CDS             complement(18405..18884)
                     /locus_tag="P5549_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HRW12317.1"
                     /translation="MKIPTSLKHKPVIVSENYENVDGRFAYNSDAKGLSLGLAQWNDR
                     GKVDISAKVWRYTGEKWSRQSEELPLHRVLDLAILICRAQKHFREAYRYEKLYNTEKT
                     TIDRVGLQGDAMTVSVCTDNPMIDEDIKLFAQTLSGDDELLGERLATLARILKEMGY"
     gene            complement(19149..20291)
                     /locus_tag="P5549_04275"
     CDS             complement(19149..20291)
                     /locus_tag="P5549_04275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRW12318.1"
                     /translation="MLIIVSIEIVLLALLVINKSSSSVFRYNQYAFMYALMIMICIVF
                     WVVLSYLEKRIEAEPRFIKMLNLAATCFIVFGMVWGAVISLMDQALYGNIIAFLVNLF
                     ITTFMFYLKPAKIFLAQVFAAAVLFVGLPYYQPSTNILIGHYANGSIFIIFLLYFART
                     NYIGFVQNFINQKIIEEKSLELTQINARLMNEIQISERVKNELATANQQLRIISSLDA
                     LTGIPNRRRLDEVLLELWSKAVAQQYAYSIMMIDIDFFKRYNDTHGHLAGDRCLQAVA
                     EVLNHCRRESLDFVARFGGEEFIFVTVGLNRQESLALAERIRAEVEALGIEHQSSSVA
                     SNITVSIGISHLYPNKEDRLDLFMEKADKALYQAKADGRNRLVLFA"
     gene            complement(20457..21344)
                     /locus_tag="P5549_04280"
     CDS             complement(20457..21344)
                     /locus_tag="P5549_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRW12319.1"
                     /translation="MNFVTYVHTNQEQVGILHEGRVMALEQLLKRPLANMLDLIGSFQ
                     TEWLGQIEEQVHSGSIAGIPSADVKLLAPIPHPRRNVICLGKNYADHAREIKITKISD
                     TYLPDQPIYFTKMASPAAGYGDTISISRKVTTQVDYEVELAVVIGKGGADIKPEQAED
                     HIFGYTIANDLSARDLQAGHKQWFKGKSLDGFCPLGPVLVHKSAIPWPVQLDIQCRVN
                     GEMRQNSNTKNMIFDISHIISDLSRGMTLRSGDIICTGTPSGVGMGFDPPAYLQDGDI
                     VECCIEKIGTLSNRIVFME"
     gene            complement(21648..21725)
                     /locus_tag="P5549_04285"
     tRNA            complement(21648..21725)
                     /locus_tag="P5549_04285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21688..21690),aa:Pro,seq:ggg)
     gene            complement(21785..23005)
                     /locus_tag="P5549_04290"
     CDS             complement(21785..23005)
                     /locus_tag="P5549_04290"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRW12320.1"
                     /translation="MALLVCKFGGSSVASIDKIKNVALRLKDMQSQGNELVVVVSAMG
                     DTTDELLELAGAISDRNSAREMDMLLATGEQQSAALLALTLNNMKCPAISLSGWQAGI
                     KTDGIHSKARITGIKPERIHNDLALGSIVVIAGFQGLSPEQNITTLGRGGSDTTAVAL
                     AAALGADRCLIFTDVNGVYTADPRIVQDARKIDRISYEEMLEMASLGAGVLQPRAAEF
                     AMHYNVDVEVLNTFNHQSGTIITEVGKMEKQRIVSGIAHDFNVARIALFDVPDQPGVA
                     MTIFKVLAESKINVDMVIQGGMRSNRNDIAFTIERDELDKALPLVNEVVAKIGASGMS
                     YGQDVAKVSIVGAGMQSNPGVAAAMFEALADAEINIHMISTSEIKVSCIIDEDEVNKA
                     VQALHAKFRLSEVE"
     gene            complement(23028..24314)
                     /locus_tag="P5549_04295"
     CDS             complement(23028..24314)
                     /locus_tag="P5549_04295"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRW12321.1"
                     /translation="MINVALLGCGTVGSGVVKLLTANGEAIARKTGAEIRIAKVLEKE
                     PEKCFALGLQAEQITSDFADILNDESITIVVELIGGMEPAFSFITAAMKKGKHIVSAN
                     KDLIAEKGKELFAIAEENQVDFYFEASVAGGIPIVYPLKQSLAGNRILEVIGILNGTT
                     NYILSKMSRESCDYRQALQEAQQLGYAEADPSADVEGMDAARKIAILSSIAFNSRVTL
                     RDVYVEGITAITLRDIQYARELGFAVKLLAIAKEVEGNVQIRVHPAFVPFNHPLAFVN
                     DVYNAVFVKGDAVGEIMHYGRGAGSLPTASAVTGDIIEIGRSLLHHSNARIGCTCFEE
                     KRILPISELNAEYYIRMTVADRPGVLAGIAGVFGDHHVSIAQVLQKTSQHDRAELIMI
                     THLVREKDMQDALTVLRSMNIIEEINNVVRLEGNHI"
     gene            complement(24334..24777)
                     /locus_tag="P5549_04300"
     CDS             complement(24334..24777)
                     /locus_tag="P5549_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRW12322.1"
                     /translation="MKEQSKFFMVRADLLPESIYKTAMAKEMLASGEVSNINQAVERL
                     EMARSTYYKYKDGVFSFHNADNLGIVNISLLLRNKSGILSAVLNCIAQLKGNILTINQ
                     NLPLHGVAYVTLSLGMEEAVLSVNEMLNRLNKIEGVINVEIIGKS"
     gene            complement(24868..26856)
                     /locus_tag="P5549_04305"
     CDS             complement(24868..26856)
                     /locus_tag="P5549_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRW12323.1"
                     /translation="MLETSMLFNVAIIFIAALIGGMIAETLKQSPLIGYMLGGVLAGP
                     YVTGLICNIELVHGFADIGVILLMFTLGIEFSLSRLKAIKGFAVGGALLQVAAVVGLS
                     ILLGGWMGLSAYESLFLGCTLSISSTMIVLRVLTDHGEMNSLHGQLMVGILIAQDLIV
                     ILMVSVLPSLQSFSPEIIPQLLKDLVLAAVIIAAVLYLAPKIIPMVLERVAQGSNNDI
                     FLILALSMGLGVAVLAQAMGLSVSLGAFLAGLFISESEYAHEILGKIISLRDAFVILF
                     FVSVGMMVNPLTLFQNLPLLLLALLLIIPGKFIVFYTISRLFKYKSRVSFYTAMGMLQ
                     TGEFSFVMAKMGLDQQLISTPLYNVILASSLISILLTPLFFNLAPGIYRRLQQNMFLQ
                     RILSDPEIDLADLPGNALCDHVIVCGYGRVGGQVGRALQQLQIPFLVIEYDHTAIAKL
                     RQARIPYIYGDASSQMVLEYANAAHARMAVLALPDTLANQQVINGLKKYNNQLLILSR
                     AHNYWEKKKLYEMGATEVIQPEEEAGLQMVRHMILHLQPDTYNVVDYLEDLYFKDYHE
                     IMRHQKTPQSEDEILKIRTFQMGDSHLNGLAIRDSGIREATGCSVVTIKRADGEIIIN
                     PHSHEILKTTDTLIMMGSLPQLELFSQYIAGQELPVSS"
     gene            complement(26997..28475)
                     /gene="spoIVA"
                     /locus_tag="P5549_04310"
     CDS             complement(26997..28475)
                     /gene="spoIVA"
                     /locus_tag="P5549_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HRW12324.1"
                     /translation="MEGNHILNDIAERTGGDIYLGVVGPVRTGKSTFIKRFMELMVLP
                     NIKDVYERERAKDELPQSGAGKTITTTEPKFVPNEAIEISTQSGLVMKVRLVDCVGYA
                     VGGALGYEEDEEPRMVRTPWFDYEVPFEEAAEIGTRKVIADHSTIGIVLVTDGSISDI
                     PRSNYVETEERVISELQELRKPFIVILNSTHPYDKQTMELAAELSEKFNVSVMPIDCA
                     TMSVEQIYGVLEEVLYEFPVQEVNIRLPLWVEELEEDFWLRQDMEDAIREILSGIKKV
                     RDIENAIGKLAEVENVSYVNLEEMDLGTGVASIEVTVPEDLFFRSLSEVSGFTVEGNH
                     DIIRLMKDLSFAKKEFDKVAAALDEVKQSGYGVVTPHLEEMILEEPELIRQGSRFGVK
                     LKAKAPSLHIIQADITTEITPIIGTEKQCEELVRYMLDEFEEDPAKIWGSNIFGKSLH
                     DLVREGIQNKLHRMPENAQHKLQDTLQRIVNEGSGGLICIII"
     gene            complement(28685..>29069)
                     /locus_tag="P5549_04315"
     CDS             complement(28685..>29069)
                     /locus_tag="P5549_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRW12325.1"
                     /translation="GLAEMIRMGVAMGGDPRTFSGLSGIGDLVVTCSSQHSRNRRAGQ
                     LLGQGKNLQETLAIIGMVVEGAHTIQIAHHLANEMNIDMPITRACYRILYENRAPREE
                     VEQLMKRQRKHEVEDINYQNHSICF"
BASE COUNT         7286 a         7432 c         6838 g         7413 t
ORIGIN      
        1 acagggtccg tcattacaga gattgacctg catatgagcg gcaaaggttc cggttgccaa
       61 ccgaatccct ttcaccctga tccgttcctc acaataatcc agcaaggctt tggccgcctc
      121 cgggtcttct gcctcagaaa aacttggccg ccgtcctttg cgggcatcac cgtacagcgt
      181 aaactgactg accatgagca tttctccgcc ggtgtcaagc agggacagat tcattttgcc
      241 ttgctcatcc tcaaatatgc gcaaattcat aatcttgtcc atcatgtaat ctgcatcctg
      301 gcagtcatcg cctttcttaa ctgccagcaa aaccagcagt cccggtccga tacgggcagt
      361 ctcccggttg tcgaccacta cgctgctgtg caaacatttt tgtattacag cccgcatcta
      421 ctcacctctt atctctcccg gcaatactct tctaacttca atgacatcct tgactttctg
      481 gattctctgt ttcacagcgg taaggtgtcc catgtccatg acttccagct tcaaattgag
      541 caaagccagt ccattcttgg taaccctgga aaacacagaa tttatctgta ttctggtatc
      601 agcgataaca ttcatgacat cggtcgtcag gcgcgggcga tccatggcct tgacctccag
      661 ttccaccaca taaacaccga tttcactggc ccactccact tccatgaccc gatccttctc
      721 attgcgcagg tagttgatta gattgggaca gtcgcggcga tggacggata cgccccggcc
      781 tctggttatg taacccagca cttcgtcacc gggcaaggga ttgcagcagt gggccagtct
      841 tatcactaca tcatccacac ctttgatctt cagggaagat gtctcacgat cacggtgtac
      901 tccggtggtc tgcggaacat ttaaaacatc gtccagttga atattctcaa agaacatttc
      961 cttcatttta ttgatcacct gattgacggt gatgctgccg tctcccaggg ctgcatacag
     1021 gtcatccgcc ttgttgagcg agaaccgggc tcctacttcc agcattttgc attctttcat
     1081 cagttcccgc ggatcaagat gctttttctt gagttccagt tccagcgtgt catgaccttt
     1141 gagaatattc tcctcccgtt tttccctctt aaaccaatgt ttgatccgac ctcgggcaga
     1201 agaggtcttg acgaaattca gccagtcggc ggtaggtcca ttggactgtt tggaggtcag
     1261 tatttctacg atatcgccat tggctagttg aaaatcgagc ggtacaatac gtccattcac
     1321 cttggccccg atacacttgt gtccgacttc agtatgaacc cggtaggcaa aatccaccgg
     1381 gcatgagcct ttgggcagtt caaataccgc tcccttgggc gtaaaaacaa acaccgtgtc
     1441 ttcgaacaga tcaattttta atgattccat gaactcgccg gtatctttga tatcctgctg
     1501 ccactccatg atctggcgca gccaggccaa tttttcatca aactttttat ctttctcgcc
     1561 ggtaatgcct tccttgtact tccagtgggc cgcaatgccc gactcagcgg tacgatgcat
     1621 ttcccaggtt ttgatctgca cttcaaaggg ctctccgccc tggccgatca gcgtcgtatg
     1681 taaagactga tacatattgg gcttgggggt ggcgatgtaa tccttgaagc gccccggcaa
     1741 gggtttccac atggtatgaa tgatccccag cacaccgtaa caatcgcgta catgttcaac
     1801 aataacccgg atggcaatct tgtcatagat ctcgtcaatg cctttgtttt gtttcagcat
     1861 ttttttatag atgctgtata tgtttttggg gcgtccggca atatcaccct ggatgccgat
     1921 ttgatcgagt ccctttgcaa tgcgctggat cagttcgtga acataggctt cccgatcttt
     1981 gcgtttggtc ttgaggcggc gggcaatttc gtagtacatc tctgaatcaa gataaaggaa
     2041 agccagatct tccaattccc atttaaactt aaagattccc aggcgatgag ccagcggtgc
     2101 aaagatatcc atggtttccc gggagatttc tttttgcttg tgctcgggtt gatattgaag
     2161 agtacgcata ttatgcaggc gatctgctag tttgatcatg atcacccgga tgtctttggc
     2221 catggcaatg aacattttcc gcaggttttc agcctgggct tcttcccggg aactgaattc
     2281 gatgcggctc aatttggtga ctccctcaac cagcaaggca atttcttctc cgaattcttc
     2341 ctgcagatca agaaaactgt aggcagtatc ttcaattaca tcatgtaaaa gggcagcaca
     2401 aatcgaagcc gtatccattt caatttcggt aagaatcatg gctacctcaa ccgggtgaat
     2461 gatatacggc tcaccggaaa tccgcttttg ccccccatgc gcagcctcag cgtaactgta
     2521 ggctttggtg atcaagtcga agttggcatt gaggtcatat tgtttaattc ggtccaaaat
     2581 gacttgtggt cccaattaca tcaccatacc agtatatttt tgcaccattg tacgccagcg
     2641 ttcccattgc tctttctctt tttttccttc ttgaaaatag ggcgacgatt ctggccgtaa
     2701 tgcgtctttg gcagtggaat gaaagtttat tgccataata ctaccgcttt tctggcactg
     2761 gcacaagtcc agatcagtca ttatgcgcaa agccgcggag agctccagac ggctgatttc
     2821 actgcctaca tcacggttca gccgggtcaa caaggctgct tcctccagcc gcagcgcttt
     2881 accactcccg gcagcagcca acgtgtgcca gataagcccc agccgggaag gttccggata
     2941 gatacgctgt aaatagctgt cgttgcggtc caggtcggca gcagtaaaac ccacttgcag
     3001 gggcagttcc aaattgtcca cataatcagc cactgctgcc agctcacagc aggcaaaagg
     3061 gctgtccagc aaggtaaatg catcgatcct gcccaattga ccggcccctg gcggatgggt
     3121 gccatccacc agcaaaagcc cggtccggtt gttgcgaaag cgctggcgga tgctgcggcg
     3181 tagatcgggc gcaatgtttc cggcttccac tgctgcgcgg ggatgttgtt gcaagactgt
     3241 attaacggac gccggccgat tcacgtagat gacatggcgt cctgtcccct cccgctggtc
     3301 ctgacttatc tggaaatgca ggcggggatt gagtttgaca gccaaccgat gaagatccgg
     3361 ttgttgccat ccagcaacca tttcctccgt ctcgggccaa aagcgaaccg ctgtttttag
     3421 agaagatggc cagcctggca aatgcaaatc acctgactct ccgggtgccg ccaggtattc
     3481 cataaaagcc tgggtgctca aataaatcct gctctcgccc tggcgaaact gccgcatgat
     3541 gatctcccgg ctcttttttt ccaattgacc gtgcatctcc accagcaacc cggctttaac
     3601 caaggcttgc agcatgacca gatgtttttt caggctgcgc agggtgggat agatgaaaag
     3661 caccggatga ccgttgacca gttcatcaat cgtcgtccgt actaccgttt ttagctgctc
     3721 ggaatacgca ttatcgccta ctccctgatc atctctccag gccggtttga tatccctgat
     3781 ttttaactgc aggctgctgc gccctttaaa gttgttctca gccagatcaa acagcagatc
     3841 ctgtttgcag atttttaatg cgggtttcat catgtctgca ccgttaaaag caatggcatc
     3901 caaatgcttg tcaccggttt tgaatttaaa atgggcttcg ttggcgccca ccagccataa
     3961 ttcatgcagc ggactgctgc gcaaaaccag cttgggggaa ggattgcctt ccccaaacgg
     4021 agccaattgt tcaatttcat tccatagctc gaggctgaga tcggcttcat cgacttccag
     4081 atcaactgga tatatctttt tccccaacgc aggcagttcc tgccccaaca tgtactcctg
     4141 caaggcctgg cagaaactgg tcagcttgtc tcggcgcaaa cccagcccgg ccgccatgcg
     4201 atggccgccg aatctttgca gatgggaact gcaggcttgc aaagcggcat ggagatcaaa
     4261 atctccgctg ctgcgggcgg agcccttgcc ctgctctcct tcccaggata tcacgatggc
     4321 agggcgatga aacttgttgg ccaaacggga agcaacaatg ccgatgacgc cttcatgcca
     4381 attctcaccg tttgccagga taaatctttg cgcttccaaa tcaggcagag caagcagggc
     4441 ctgctcataa attgactctt caatctcccg acgttcatca ttcaatcggc acaattcttc
     4501 cgccagcaac cgggcttctt ctggattctt gctcaacagc aacgctacgc tttggcgggc
     4561 ggaatcaatg cgtccggcgg agttgagccg gggggccaga ataaaaccga tgtgaacggc
     4621 agtaatgggc ttgccgcaca ggccggtctt ttccagcagg gcaatcaaac caggccgggt
     4681 cgtgtttccc agcgcgacca gaccgtattt caccagaatg cggttttcat cccgcaaggg
     4741 cacgatatct gccacggtcg ccaaggcaac cagttccagc cagtcctgtc cgggctccat
     4801 tccctttgcg cgcatcaagg cacaggccag tttataactg accccgacac ctgccaaatt
     4861 ggcaatagac ggcaattggt cattcttggg attgataacc gcatgggcag ccggctgttt
     4921 ttctgccggt gtatggtgat cggtgatgat caaatccatc cccaatgagc gggcaagctc
     4981 cgcttccgcc agcgaagaga tgccgcagtc aactgtgatg acgagattgc agccccgttc
     5041 actgagcagc tgcagagcct ctgcattcaa accatagccc tcccgaaaac ggttgggcac
     5101 atagtaatca acaggataac ccattcgctc aaagcactcc attaaaatga cgatgctgca
     5161 cacaccgtcg gcatcataat ctccgtaaac cacaattttt tccccggccg ccatagcctt
     5221 gtcaatgcgc tccagggctt gcttcatccc cttcatcttg agcggatctt ccagcatgtt
     5281 caattgcgga tataaaaaac gactagcctg ggctgcattt tgattgcctc tttgcaccaa
     5341 caggcgtgcc accggcagat ttatattgag ctcacggctt attgtggatg caatttcatc
     5401 gttatagggt tttaattgcc agatactatg caaatttgac ctcctgtttc cagcagatga
     5461 atatcacagc gatccgccgg tattatttga ttatatatga gcggaggcag attgcgggag
     5521 ttgatttgga aaaactaaaa atttttcctt gcattgtgac agttccagac atgtatccat
     5581 catttcttca tatataaata atgtctgcac ttgtgcaaat actgtttgac aagcgcctgg
     5641 acaaaacaaa ttgtgtggag gtcgtatgat ggcaggaaca agacatcttt ttcccggggc
     5701 aaacagttgt tacggttttt tttctttcta tgaccaaatc gtgccgcccc aggttaaaac
     5761 caaggtcatc ctcaaaggag gaccgggaac cggaaaatct acttttatga aaagcattgc
     5821 agacgatttg ggttcgcagc aaattgaggc tgaatgccac tggtgttcct ctgacaacaa
     5881 ttctctggac gcggtcgtag gcgggcatgg ccaggtttgt atcctggacg gcacttctcc
     5941 tcatgttgtc gacccgcgct atcccggggc ggtggatcga atcattaatc tgggtgaata
     6001 ctgggatgct tccttgctca agaaaaaccg ggctgacatt gagaagctga cggatctgat
     6061 cggactgtct tttcaacggg ctacaaccgt ctggcagaat ccaaactggc cttccgggaa
     6121 tggaaatcct accttcagga agcccgggac gagcaagcag tcaaacgcaa cattctggcg
     6181 ctggcggaag aatttttaca ggatcatcga gcggccgggg gcaggctgcg tcatttgttt
     6241 gccggcgcca tcactcctgc cgggattgct tcccaggctg agagtctgtt ggaaagcgat
     6301 ctgaccgtgc tgggagtgaa gggcagtccg ggcggcggcg gtaaggagct catgcgttat
     6361 atcttgacca gaattgagct gggcggggtc ttcgctgaag tctttcactg tccgtttgat
     6421 cccgaagaaa ttgatatgat catcatccct gacaacaaaa ccgcgctggt ggatttctca
     6481 tctgccgtca ttgattacga gacagcctgg ccgacgcgca ggtttaagcg ttttcttgat
     6541 tttgaccagt tcctggatca gtctgtactg gactcccggg ccaaggcgat tttttccagc
     6601 cgggaacggt ttgcggcggg aatacgggca gcagtcgcgc atatcaagac ggccaaggag
     6661 tggcatgacg agttggagag cttttatgtg ccggccatgg attttgagcg gatcgaggag
     6721 gttcggcagg aactgctgac tgaactgctg gcggaactgc aataactggt agtggctctg
     6781 ccgttcctgg catcgttgct caccagcgca cggctgccgc cttaccccct gcgctatact
     6841 aaagcataaa cctctcgtga cggttttctg tttgggctct gccattggct tcgccgtcag
     6901 gggctcggct tcgcctttgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 ntctgtttgg gctctgccat tggcttcgcc gtcaggggct cggcttcgcc tttgccgggc
     7081 ttcgccgtta ggggctcggc ttcgcctttg cccgggcttc gccgtcaggg gctcggcttc
     7141 gcctttgccg ggcttcgccg tcagttttag tatggtttgg ccattagaag agggttacgg
     7201 ggacttcttc gctggctggc aggccttgtt tttcgtaggg gatgtggata tagccatcgg
     7261 cctgggcgag gatgttcatc aggccggact tgcccagcaa gggtttgacc gccagtccgg
     7321 tttcacagtt gatcaattgg gctgggataa aatcgtcgcg tccggcctgg gaagctacgt
     7381 tgacggtcag gcggcctttt tcctgccaag ctttgtactg gcgcaacagc ggcgcacaaa
     7441 tgatatgaaa aatcatcagg gctgatacgg gatgaccagg cagtccgatg atcagctgcg
     7501 aaccggtttt gactgccagg gtgggcttgc cgggtttgat ggcgatgccg tggaaaagca
     7561 actggctgtc cggcattgac agcatggctt ccagggtata gtcggcgatc ccgatggaac
     7621 tgccgcctga taccaataat aaatcatttt ctttgagtcc aaaagcaatg gcttgtttta
     7681 aagacgccag atcatctttt accagcggat atggacaggc aactgccccg catgactgga
     7741 gcgcagcgct gatggcccag gtgttgacgt ctctgatttg accaggccgg ggttgttcct
     7801 gaaaagggat gatctcgtcg ccggttgaca atataccgac atgatacggg ctgtataccg
     7861 acacttcttt tatgcccaat gaagccagca gtccgatgtc ctggggacgt actttgtgtc
     7921 cgcgggaaaa tatcaaatct ccggcagcaa tatcctctcc tgactgcatc acattttccc
     7981 aggcacccac cgggcgaaaa accagcacgg tatcttcgtc caatcgttcc gtatattcca
     8041 ccatgatcac tgcattgctg ccctccggca acatgcctcc ggtgggcatc caggcgcatt
     8101 gaccggggcc aaccttcaaa ggggtttcct ttcccatgga taccgaacca atataggcaa
     8161 gcattcccgg caggctctcg ctgctgccat aactgtctgt gcagttgaac gcataaccgt
     8221 ctactgtgga acgggtaaag gccggcatgt tttccggact gattatgtca tctgccagaa
     8281 tgcggtcaaa gcagtcctgc agggctatca tttcggttga tggcagtgaa aagtgttcac
     8341 gtaaaatctc ccgcgcattc tgataagtca ggacttttag aaattcctgc atgcttgact
     8401 ccttatcatt ttatttcgtt cctgcaatcc attatactta aatccaggac tagctacaaa
     8461 tgaagcaaca acgattatct taatggagga tgcccatgag cagaaatatt tatatcgaca
     8521 acatgcccca ggagaaagcg ctgcaattgt ttatggacaa actgcaatcc tgcggttggt
     8581 ttcaacgtac cgtggaatac tccccggtga gagagtcact gtatcgggtc acggcggagc
     8641 cggtcattgc cagacgttct tcgccccact atgtggcttc ggccatggat ggagtggcgg
     8701 taaagtctgc catgacagtc ggtgctacgg aagccagtcc agtcaacatc atggccggcg
     8761 actatctgga agttgatacg ggtgatttgg ttcccaagga atatgatgcg gtaattatga
     8821 tcgaagaggt taatttcttt gatgatcata tccaaatcat caaggcagca gtgccctggc
     8881 aacatattcg ttcgattggc gaagacatgg tagaaggtga tatggttctg tccagtctgc
     8941 gcgcaattgg tccttatgaa ctggcagcac tgatcacggc aggcgttgaa acggtccccg
     9001 ttataaaaaa accagtgctg gctgtaattc cgaccggaac agaattggtt gatacagccg
     9061 gcatcaccat ggcgcctgga gatatcgtgg aatcaaacag ttacatgctg atgggactgg
     9121 ctcgtgaatg gggtgccgag tgtctgcgtc atgacattgt gatcgacgac cgtgagcaga
     9181 tcaggcaggc tgttttggaa gtcgagccgc tggctgacat catcgtaatt tgttcgggat
     9241 cctccgccgg tcgagaagac tatacagccg gcattgtcag agaactgggt gaaatcctgg
     9301 tgcatggcgt ggcggtccgg cccggcaagc cagctatatt aggtattatc gaacaaaaac
     9361 cagtgatcgg cgttcccggt tatccaattt cagctggatt gatatttaat ctttttgccc
     9421 gaccgatcct gtatcgcaaa gcaggaaggc atcttcccgg agataaaacc atgaccgcaa
     9481 ccatatctaa aaagttgcct tcccccatgg gcgtggatga atacattaat gtgaatgcag
     9541 ccgtgatcaa cgacagatat attgcttatc cgctcaatcg cggggcagga ctgagcagca
     9601 ttctggtgaa atccgacgga acagtgcatg tcgaacgagg ccaggaagga ctgcaagccg
     9661 gtgcaggctg taagatatca cttcatcgct cccgccgtga gatgcaaaac agtttgatca
     9721 ccctgggcag ccatgatatg agcattgatt ttctgattga tatcctctat cgtgatcacg
     9781 gtatccgcct ggtctccacc aacgttggca gtatgggcgg catcatggca ctgcgacgca
     9841 aagaaaccca ttttgccggt ctgcatttgc tggatgaaga gggcggctat aatatcagtt
     9901 atttacacaa atacctgcca aacgaaaaat atctgctggt caacctggtt aaacgccatc
     9961 agggactggt ggttgcaccc ggcaatcccc ttggcattaa gagtatcagc gatttaacca
    10021 gaagcgacct gcgttacatc aatcgtcaaa aaggtgccgg caccaggatt ctgctggatc
    10081 atttgttaaa gcagaaaggg atatcgtcag ccgacattaa cggctatcag cgcgaggaat
    10141 acacccatct ggccgttgcc gccgccataa aaaacgatgc ctgtgatacc ggcatggcca
    10201 tttttgccag tgccaaagcc atgggtcttg acttcgtgcc gatcgatgta gaacgttacg
    10261 atctttgtat tctgaccgag accatgccgg accagcagct ggaatgcttg ctgagcgtaa
    10321 tccgcagtga tgaatttaaa gaaagggtac ttttttttgg tggttatgaa accgatctga
    10381 ccggacaaat tatatatcaa caaggttatt aaccaaggtt ttcatgaaaa gttaaaatat
    10441 actgtgaaat attccctgat tttgctgtta aaatagagga ataattaaac ttttatcgaa
    10501 ttagttcaat gtaaatataa ttataaagaa agctctgaag ctgtcagcct aaaacgcaaa
    10561 gtcaaggcaa ccagccattg ggtagacttg gaaacgggtt tatctcccct tattgtggaa
    10621 aggagccata attgcactgt tctgtttttg agagcagtgt gtgtttgtgg ttttttgtct
    10681 ttcaataagg ggtttatttt ttagatatat tttttaggcg atatttatcc tcttaaggag
    10741 gtgaaaggtt ttcacttttt agtggctgga ttacgattgt tgctccattg aaagggtgat
    10801 tggagtatac gatccgggtc ctgattctgg ttagcatttc cacagtgttt taaaattcgg
    10861 gaggggaaag gagagtgttc gtactatgaa ggttacccgc agacagttcc ttaaggtaac
    10921 cggagctaca gcggctgcca tggcagtaac cgatctcggt ttcaatatga atgccaccgc
    10981 cagcgcaatc aaagaattac gtatcaaaaa cgtaactcca acgcctacag tttgtcccta
    11041 ttgtgcatcg ggatgcggtt tggttgttta tgcacaaaag gatagtgccg ggaagtttca
    11101 aaaattgctc agtgtacatg gagatcctga taatcctatc aatattggag gagcttgttc
    11161 aaaaggcgcc gccatgttca acctgaggga aatttacgat ccgcaaaccg gcgaacagat
    11221 catcaaccct aaaagagtgc agaagccatt atacagagct ccgggaacgg ataaatgggt
    11281 agaaaaagat tgggattgga tgctggacac cattgccgag agaatcaagg aaaccagaga
    11341 cagcagcttt atccatacgg aaaaactggc tgacggcaac gatgtcattg taaaccgtac
    11401 tgagaaaata gcttctttag gaggatccgg actggataat gaagagtgtt atctgttgtc
    11461 caaaatgatg cgttccctgg ggctcgtgta tcttgaaacc caggcccgta tctgacactc
    11521 cgcaacggtg gcaggactgt caccttcatt tggccggggt gttatgacca atcatgtagt
    11581 cgatttgaaa aacactgatt gtgcactaat catgggcagt aatgcagcgg agaatcaccc
    11641 catcagcatg aaatggctga ttcgggctcg tgagaacaga ggcgccaaaa ttatccatgt
    11701 tgatccccgc tatactcgca catcagcccg ggcggacttg tatgcaccgt tacgctcagg
    11761 aacggatatc gcatttttgg gtggtatgat caaatatatc atagataacg aactctatca
    11821 caaggattat gtagtcaact acaccaatgc tgcttacctt gtgaaggacg aatatggatt
    11881 cgaagacgga ctgttttccg gctacgacca ggaaaaccgc aaatatgatg tcagtagttg
    11941 ggactacaaa tatggcgaag acggcctgcc tttgaccgat cccacgttgc aggatccgcg
    12001 ctgcgttttc caactaatga aaaagcatta tgaacgctac gatatagatt ccgtgtgcaa
    12061 aattaccggt acccctaaaa acaagtatct ggaagtcttg gatatgttct gtgcgaccgg
    12121 tgccccgaac aaaaccggca ccattatgta tgccatggga atcacccagc atacggtcgg
    12181 aagtcaaaat gtaagatctt tcgcagtcgt acagctgctg ctcggcaata tgggcagacc
    12241 cggcggcggt gtcaacgcac tgcgcgggga aaacaatgtg caaggtgcaa ctgatatggc
    12301 tttgcttttc cacattatcc caggctatat tgattcaccg atgaacaatg ccgcccacaa
    12361 agatttgatt tcttttgtga gatccgttac tccgggagcg gccaagcttc cctttaccaa
    12421 tctggaagaa ttacgtgagc tggtaaaaga aggtttcccc aattctggct ggaaaattca
    12481 cagttccaaa tgggtggtca gcttgcttaa agcctggtac ggtgaagctg ccaatgccgg
    12541 caatgattat gcctatcact atctgcccaa acgggatgaa aagaaaaact attcccacat
    12601 gcaacttttt gaagccatgc accgcgggga gattgacggt ctgtttgtca acggcaccaa
    12661 cccggtcgta ggcggcccta atgccaacaa ggaacatgaa gccctgacca agctgaaatg
    12721 gatggtttcc attgacctgt ggctgaatga aacagccgat tactggactc accaggcttg
    12781 ggacagaccg gtagaaaatc ccaatgtggc aaaatatact cctgctgaaa tcggtacgga
    12841 agtctttttc ctgccagccg cggctgtata tgaaaaagaa ggtaccgcag ctcaaaccgg
    12901 tcgctgggta caattccgct ggaaaggtgc agatccggtc ggagaatcca aatccgatct
    12961 gtggatctac aatgaactcg gcaaacggat caagaagatc tatgcaggaa gcacacgcaa
    13021 agaagatgag cccattgtca atatgacctg ggattatgac aacgaacacg gcgaagacga
    13081 tatcatgaaa gtagcactcg agctctgtgg atttaatgtt gccgacggaa agccggtgga
    13141 aggctttgcc aagctgaaag acgacggttc cacggcctgc ggcaactggg tatattgcgg
    13201 agcgatgacc ttcaaagacg gcaagaccat ttacaaacca cagttgcgca aaaaggatga
    13261 tcccagcgga ttgggccttt atcccgagtg ggggtgggca tggcctatga atcgcagaat
    13321 cgtttacaac cgctgttcgg tccagcccga cggcgtaacc ccctggccgg gtgatgaaga
    13381 acgccagttg gtcagatggg atccgaatgc cattgccgat ccgaccaaac cagaagtatt
    13441 aggcaaatgg gttggcgtgg atgtccctga tttcataggc actaccggtc cggacaaacc
    13501 ggccttcacc aaaccgttta ttatgcgtcc ggaaggacac ggttgtttgt tcgctgccaa
    13561 aacttccatg aaagacggtc ctttccccga acactatgaa ccttgggaaa gcccgaccgc
    13621 aaacaaaatg aacagccata atctcaaccc ggctacaaaa gtaattgaac cggacaaaca
    13681 aggtgatgct gaaaaatatc ccatcattgg gacgacctac cgtgtttcag agcattggca
    13741 gaccggtgct ttaacacgta acctgccctg gctggcggaa ctgatgccca acatgatggt
    13801 tgagctcagc gaagaactgg ccaatgaaaa agggattaaa aacggtcacg acgtgatcat
    13861 ttcaaccata cggggtgacg tcaaagctgt agcctgtgtt accaaacgct ttaagccact
    13921 cgttatacat ggcaaacatg tacatgagat cggtttgctc tggcaatacg gattcaaggg
    13981 cttcgctacc ggcgacattg ccaactggct gacgccgcat attggtgatg cgaatacgat
    14041 gattcccgaa tacaaagcat ggctatgcga cataaggagg gcttaacgat ggctaatctg
    14101 acaaatcttt atgatgttag caaatgtacg gcctgtcgcg gttgccagat agcctgcaaa
    14161 gattggaacc agcttccggc cagcatcgag ccttttaaag gcaattatca gacccatgaa
    14221 gacaccgatg gcgatacttt taccattatt aaattctttg aaaccgaaga tccagtaacc
    14281 ggagtaaact ggagctttat caaacaccaa tgcatgcatt gttttgagcc ggcctgcatg
    14341 atggcctgcc ccaagggtgc ctataccaaa acagaaaccg gggctaccgt gcatgatccg
    14401 gacaagtgta tcggctgtca gtactgcaca tatgcctgtc cgtttgaaat ccccaaatat
    14461 cgcaagcggg aggataaaat ttccaaatgt accctgtgtg tcgaccggac ggtggaaggt
    14521 ctgacccctg cttgtgccag aacctgccct accggtgccc tgacttttgg tgatcgtgat
    14581 gaactgctgc aaaaagcgga agatcgcgcc aaatatctgc gtgaaaatgg ctatcccaac
    14641 gctactatct acggcaagct ggagttgggc ggtttgaaca agttatatgt gttgacggat
    14701 accccggaca aatttggtct gccggttgat ccgcatattc ccaagaacat ttctgcctgg
    14761 cagaatgttg tccagcctta ctttggctgg ctgattccct tgatgctggg tgcttcggca
    14821 gtcagtttcg ttactacccg cttgttggcc aacaaacatg gcggacacgg cgaacacgag
    14881 gaaggaggtc atgaataatg caatggctta ccgaatataa accagatgtc gaaagagtgg
    14941 agcgcttcaa ttttgttgct cgccttaccc attggggcca tactgtgact ttcctgctgt
    15001 gcctcttcac cggtctggtc ttgttcctgg atggggtgga ctggctggca gccattttcg
    15061 gcggttatgg cggagctgga ctggtacaca gaatcgcagc agtattgtta accatttttc
    15121 ttgcggtttt tgtagtcttt gactttaaag gcatcatcaa ctggatcaag gacatcctgc
    15181 gctttggtat gaatgacatc aaatttgtca tgaaattccc gctggagttc tttggctttc
    15241 cggtcaaggt gcccccgcag acccgcttta acggcggtga aaaaggcaac tccattgtga
    15301 ctcccaccat ggtcatcctg ctggtcatca gcgggtacat catgtggttc ccggctattt
    15361 tcccggctgg tttggtgaga gtggcttatc ccacccatga cattgccatg atcctggcga
    15421 cctgtatggt atgcatgcac gcttacctgg gttccttcca tcccggttcc ggagaatcct
    15481 tctggggcat gtggaaaggg acggtccgct ccgaatgggc caagcatcat catgccatct
    15541 ggtatgatga aacctatggc gacaaaacag atgccgaata aataatccaa atatgatata
    15601 atcgattaaa caagaagggg agtttactcc ccttcttgat gcttccggac caacgtgtta
    15661 tgattacagg cagcaactcc ggcatgacgt catgttgtag ccggcacgta ccggctttca
    15721 ttatcgactc attttaaagg aggatattca tgaacaggca aactccagta gccctccctg
    15781 aaggatatgt tgagttttac agcaaccttg aaaactggca gaatgaacag gaaatcgtat
    15841 taaagaaaaa ccattctccg gagcatattg aagtccgcaa gctcctggcc aacagtcaaa
    15901 aacatttgat gaaacaacat cgttttacaa ttgatccgca acaactaaga gagacatacc
    15961 tggctctggt tgtttttctg caaagcaacc ggcccatcac cgtcgagatg atcgagattt
    16021 tgcgcaaggc tgctgatcag ctggactttg ataaccttgc agcaatcctg ctcgacaatg
    16081 atccgaccta ttttattgaa atggcagagg atttggggat cgccagtgaa ttactggtct
    16141 ttacggttga tcatgcgctg cgtccttatt tgcgaatgct ggcccagcca taccatcatg
    16201 aattcagcca ggaagaaatc ggcttctgga gtcaggccgc cttttgtccc ttctgtggca
    16261 gcaaatcgca ttttagccga atgcgcgaag cagacggcaa gcgcttgatg ttttgcgacc
    16321 gctgttttac cgaatgggaa agccgcagca tttattgtgt ccactgcggg aatgatgagc
    16381 ccaataccat caattatatc agcgtcgaag atgaccccgc ttatcaaatc tacacctgtg
    16441 ataaatgcaa aggttatctg aaaacctatg atgaacgaac aactggcaaa accactgatc
    16501 tgttcatcgc caatatcgag accatttatc tggatctgct ggcccaggaa aaaggatatg
    16561 tcaatcatga tgcggattga tgccgtcaga taagccataa ggctcaagca gaaaaaagtt
    16621 tcctcgattg caattttcga ggaaactttt tttatcgcat ctttttactt tttctgttta
    16681 agcggacggc ctatggtatg ctccaacacc agagaagtaa cgattcccac cacaaaacca
    16741 ttgctgagga tgggacgcag gctgtaagga taactggcgg tgatctccgc gggcaggaat
    16801 gaaatgatga tccccatcag aatcggcaga ctgatcatca gcgcattgtc aaagcgcggt
    16861 tcgcccagtt cctgcaaggc atcaaaagcc gtcatcaaac ctgccgccac ttgcgtacac
    16921 atggtgtaga aaagaacaca gccaatcacc gccgcaggga taaaggaaag ataaaagata
    16981 atccgcggca aaaatgccag cgccagcatc gccacgccgg cagaaatcaa actgcgacgc
    17041 gccgcacagc cggtcgccgc aatcaagccc gggctgaaag agaagttgac tggtccaatg
    17101 acccccatcc atcctgctaa aacattgccg atgccggtag ccgcaatccc ccgggtaata
    17161 cgacgcggca ttgcctcagc ctttaacacc agtccaaccg cctgaatcga gcccagatcg
    17221 ttgattgaca ggcccaggaa gcagagtata aaagccacca gcactcccgg ttcaatgacc
    17281 agcgaagtat ttaaatggct gaaaaacaac gcgaccggtt ggatattttc cacactggca
    17341 tgcgcagccc agcccgggtc gattaaaaaa taggccaaac tgcccaatac catggcgacc
    17401 agcaccaggg tgtttttcag caatccttgc agataacgtc cgcccacaaa catcaacagc
    17461 ataaaaacaa aggcaaatat cagccgccgc ccggcgcccg gaacttctgt accaccgatg
    17521 accaggttgg taatggtagg cagaatggta aaggcaacca acaagaggat gaccgccacc
    17581 acccgggggg taaacaacga ctgcaagcgt ccaaaaaggc cggttaccgc cagcagagcc
    17641 agcagggcgc cgcatattag caaactggta tagatcgcag caggcgtgct ttccaatgaa
    17701 gccaggatgc caataaaaag aatggtagag gggcccagga caaccggcaa acgatgcccc
    17761 caaaaaacct gtatcagcag cgcaactccc atcacggcaa acagcttttg cagatacaat
    17821 atctccagtg ccggggagtc catccctccc agcaccttgc cgaaagtgat caccgccggc
    17881 agactgaccg ccagccactg caggccatac aacccatttt ctttccaaac cggcttgtct
    17941 tcaatctgat attttaattc caacgacaaa cagtcccccc tggtcaatac cgaatatttt
    18001 agtcaagcga tcagcttcat ctttgctcac ctgtcaggca aacaaccggt ttctttttaa
    18061 tacagctgct tggcctcaaa tctttccagc caaatgtctt tgagtgtttt taaatcatca
    18121 cgaaattctt tgggcgtttg ctcttccatc ttttcaagtt cctccagtac ttccagcgta
    18181 aagctttggg cgccgtattt ttcccactcg gacagcaatt tttcttccac acagctgccg
    18241 gtttgctggg aaaacgcaaa gcggctctga taggcacgca ggttattgac cggcaatatg
    18301 agcgttttcc cattggctgt attttttatg gcgcaaatgc cgccaatgtc tttttgttct
    18361 ttataggcag cccgcagctc tttgcggttt tgtttatcca taaatcagta ccccatttct
    18421 tttaagatcc gcgccagcgt cgccagacgt tctccgagca attcatcatc cccactgagc
    18481 gtctgcgcaa atagtttaat atcttcatcg atcatcggat tgtctgtgca aaccgaaacc
    18541 gtcatggcat caccctgcaa accaacccgg tcgatggtgg ttttttcagt gttatacaat
    18601 ttttcataac gatatgcttc gcgaaaatgt ttttgcgccc gacagatcaa tatcgccaga
    18661 tcaagaacac gatggagcgg caattcttcc gattgacgcg accatttttc accagtgtag
    18721 cgccagactt tggctgagat atctactttt ccccggtcat tccactgggc cagtcccagc
    18781 gataaacctt tggcgtcaga attgtacgca aatctcccgt caacattttc atagttttct
    18841 gaaacgatta ctggcttatg tttcaaggac gtaggtattt tcattttcat ttctccattc
    18901 atgtttagta atttagtaat ttagtaaata acacgattca tactacaatt tgaatgaagc
    18961 gctgtcaaga aaaacgtctc cctataaata tctcatcaaa tacaaaaaag aagctcctgt
    19021 ccgatcagac agactttgct cccctgcggt atgtatgatt catttttacc cgagtgatgg
    19081 cacatgccca tgccgattaa aacttctggg ataaatgaaa cggaattatg acgtgcaatc
    19141 ataacgatct acgcaaacaa taccaggcgg tttcttccgt ccgctttggc ctgataaaga
    19201 gctttatcag ccttttccat aaagagatcc agcctgtctt ctttattcgg atacaaatgg
    19261 ctgataccga tactgacggt aatatttgag gcaacggagg agctttgatg ctcgataccc
    19321 aatgcttcta cttctgccct tattctctcg gccagcgcca aactttcttg cctgtttaac
    19381 ccaaccgtta cgaatataaa ttcttccccg ccgaaacggg cgacaaagtc taaagattct
    19441 cgccggcagt ggtttaaaac ctcggctacg gcctgcaggc aacgatctcc ggccaaatgt
    19501 ccatgtgtat cgttataacg cttaaaaaaa tcaatatcga tcatcattat ggaataggcg
    19561 tactgttggg caactgcctt gctccataat tccagcaata cttcatccag tctgcgtcga
    19621 ttcggtatcc cggttaaagc atccagactt gaaataatgc gaagttgttg gttggctgtt
    19681 gccaattcat tttttacccg ttcgctaatc tgaatttcat tcatcagcct ggcgttgatc
    19741 tgcgttaatt caagcgactt ttcttcaatg atcttctggt tgataaagtt ttgaacaaat
    19801 cctatataat tggtacgggc gaaatacaaa agaaaaatta taaaaatgct gccgttggca
    19861 tagtgaccga ttaagatatt agtcgaaggc tgataatagg gcaagcccac aaataaaaca
    19921 gccgcagcaa atacttgtgc taaaaaaatt ttagccggtt ttaagtaaaa catgaatgta
    19981 gtaataaaca ggtttaccag aaaagcaatg atatttccat aaagagcctg atccatcaaa
    20041 gatattacgg cgccccatac cataccaaaa acaataaagc atgttgcagc cagattcagc
    20101 atttttataa agcgcggttc agcctcgatt cttttctcca gatacgacag aacaacccaa
    20161 aagacaatgc agatcataat catcagggca tacataaaag catactgatt gtatctgaaa
    20221 acactgctgc tgctcttgtt aatcaccaaa agagctaata agacgatttc tatactcaca
    20281 attataagca caaacgactt ccctctctgt aaattaaagc gggaaatctc caaggcaaat
    20341 tgatgttgat agacagggtc aatcttaggg attgcattgt acaaaatggt ccacccccga
    20401 atttttcatt cgtcttgtgc aaattgctta cagataatgt atgccgatca tccttattat
    20461 tccataaaaa caatccgatt gctcagggtt ccgatttttt cgatacaaca ttcaacgata
    20521 tccccgtcct gcaggtaagc cggtggatca aatcccatcc ccacgccact gggcgtgccg
    20581 gtgcagataa tatccccaga ccgcaaggtc atcccccggg acaaatcgct gataatatga
    20641 gaaatatcga agatcatgtt tttcgtattg gaattctgtc tcatctcccc attgacgcga
    20701 cactgaatgt ccagctgtac cggccaggga atagcacttt tatgcaccaa caccggtccc
    20761 aacggacaga agccatccag gctcttgcct ttaaaccact gtttgtgacc ggcctgcaga
    20821 tcccgcgcgg aaaggtcatt ggcaatggta tagccaaaaa tatgatcctc cgcttgttcg
    20881 ggcttgatgt cagcgccgcc cttgccaatc actaccgcca attctacttc ataatcaacc
    20941 tgcgtggtaa cttttcgtga aatcgaaatg gtatcaccgt accccgcagc cggagatgcc
    21001 attttggtaa aatagatggg ctgatccggc aggtaggtat ccgaaatttt agtgatctta
    21061 atttccctgg catgatcagc ataattttta cccaggcaaa taacgttgcg ccggggatgc
    21121 ggtatgggcg ccagcaattt gacgtctgct gacggtatcc cggcaatact tccactgtga
    21181 acctgttctt cgatctgccc cagccattct gtttgaaagg aaccaatcaa gtccagcata
    21241 ttggcaagcg ggcgtttgag caactgctcc agcgccatga cccgcccttc atgcaagatc
    21301 cccacctgtt cctggtttgt gtgtacatag gttacaaagt tcattctatc ttgctcttgc
    21361 tccttctttt tggggttcag taatgatatt tcctgtcgtc caatacagaa aatgtttcac
    21421 gtgaaacatt ctttgtttgg taatatccaa atatagccag aatatctgca ttctttattt
    21481 ttatgatact cctttggtaa gaaaaaccgt acttttattt gcattatttt caatcacaat
    21541 ctcgatcgca actggtcatt ggtcctgttt ttatgattct ggcaaccgaa tgctacaaaa
    21601 aaaccacctt gttatgaaaa caaagtggtc tgctctcgcc ttttaaatgg tcggagtgac
    21661 tggatttgaa ccagcggcct ctacgtcccc aacgtagcgc tcataccaaa ctgagctaca
    21721 ccccgacgca aagaatattc tataacagaa ccttctttaa gtcaagcaaa agctcgaata
    21781 atggctattc cacttcgctc agccggaatt tggcgtgcag ggcctggacg gctttattga
    21841 cctcgtcttc atcgataatg caggaaacct tgatttccga ggtgctgatc atatggatgt
    21901 tgatttcggc atcagccagc gcctcaaaca tggcggcagc cacaccgggg ttgctctgca
    21961 tgccggcgcc gacgatggat actttggcca catcttggcc ataggacatc ccggatgcac
    22021 cgatcttggc gaccacttcg ttgaccagcg gcagcgcctt gtccagttca tcccgttcga
    22081 tggtaaaggc gatatcattg cggttggaac gcattcctcc ctggatgacc atatctacat
    22141 tgattttgct ctcggccagc accttgaaga tggtcatggc taccccgggc tggtcgggca
    22201 catcaaacag ggcgatcctg gctacgttaa aatcatgggc gattccgcta acgattcttt
    22261 gcttttccat tttgcctacc tccgtaatta tggttcctga ttgatggtta aaagtattca
    22321 gcacttccac atccacatta taatgcatgg caaactctgc tgcccggggc tgcaaaacac
    22381 cggcccccag actggccatc tccagcatct cttcatagga tatgcgatcg attttgcggg
    22441 catcctgaac gattctgggg tcagcggtat agactccgtt gacatcggta aatatcaggc
    22501 agcgatccgc tcccagggct gctgccaagg caaccgcggt ggtatccgaa ccgcccctgc
    22561 ccagggtggt gatgttctgc tcaggcgaca gcccctgaaa gccggcaatc accacgatac
    22621 ttcccaaggc cagatcgttg tggattcttt ccggtttaat cccggtgatg cgggctttgc
    22681 tgtgaattcc gtcggtttta atccctgcct gccatccgct cagagagatg gccgggcatt
    22741 tcatattgtt gagggtgagg gccagtaaag cggcgctttg ctgttcacca gtagccagca
    22801 gcatatccat ttcccgggca ctgttgcggt cgctgattgc ccccgccaat tccagcaatt
    22861 catcggtggt gtcccccatg gcggaaacaa ccaccaccag ttcattgccc tggctttgca
    22921 tgtcttttaa acgcaaggcg acgtttttga tcttgtcaat tgatgcaact gaactgcctc
    22981 caaatttgca taccaataaa gccaattttg ttcaactccc cataatgcta tatatgattg
    23041 ccttccagac ggaccacgtt gttgatttcc tcaatgatgt tcatgctgcg cagcaccgtc
    23101 aaggcatcct gcatgtcttt ttccctgacc agatgggtaa tcatgatgag ctccgcccga
    23161 tcatgctgac tggttttctg caacacctgg gcgatactga catggtgatc gccgaatacg
    23221 ccggcaattc cggccaacac gccggggcga tcggccaccg tcatgcggat gtaatactcg
    23281 gcatttaatt cgctgatggg caggatccgt ttttcttcaa aacaggtgca gccaattctg
    23341 gcattgctgt gatgcagcaa actgcggcca atctcgatga tatctccggt caccgcgctg
    23401 gccgtcggca aggaacctgc gccccgcccg tagtgcatta tttctcccac cgcgtcacct
    23461 ttgacaaaca ctgcattgta aacatcattg acaaaagcca ggggatgatt gaagggtaca
    23521 aaggccggat gaacgcgaat ctgcacattg ccctccactt ccttggcaat cgccagcagt
    23581 ttgactgcaa agcccagttc acgagcatat tggatatcgc gcagggtgat ggcagtgatg
    23641 ccttccacat aaacgtcccg caaggtcacc cggctgttaa aagcgatgga agaaagtatg
    23701 gcgattttac gggctgcatc catgccttcc acatccgcac tgggatctgc ttcggcatag
    23761 cccagctgct gggcttcctg caaagcttgc cggtagtcac agctctcccg gctcattttg
    23821 ctgaggatat aattggtggt accattcaag atgccgatca cttcgagaat gcggttgccg
    23881 gccaaggatt gcttcaaggg gtaaacaata gggatacccc cggctacgct ggcttcaaaa
    23941 taaaaatcaa cctggttttc ttccgcgatg gcaaagagtt ccttgccctt ctcggcaatc
    24001 aaatctttgt tggcactgac aatatgtttg ccttttttca tggctgctgt aataaagctg
    24061 aaggctggct ccattccgcc gatcaattca accacaatcg taatgctttc atcattgagg
    24121 atgtctgcaa aatcactggt aatctgctcg gcctgcagac ccagggcaaa acatttttcc
    24181 ggttctttct ccaaaacctt ggcaattctt atctctgctc cggttttgcg ggcaatagct
    24241 tcgccgttgg ccgtcaataa cttgaccact cccgaaccga ccgtgccgca acctagcaag
    24301 gctacgttta tcatagaatt ttcctcccgc tgcttaactt tttccgatga tttcgacgtt
    24361 gataacgccc tcgatcttgt tcagccggtt tagcatctcg ttaaccgata aaaccgcttc
    24421 ctccatgccc agcgacaagg tcacataggc caccccgtgc agaggcagat tttgattgat
    24481 ggtcaggatg ttccctttta actgggcgat gcaattcagc accgctgaaa gtataccgga
    24541 tttattgcga agcagcaagg atatgttgac aatccccagg ttgtccgcgt tatgaaaaga
    24601 gaagaccccg tctttgtatt tataataggt actgcgggcc atttccaggc gttcaaccgc
    24661 ttgattgata ttgctgactt ctccgctggc caacatttcc ttggccatgg cggtcttata
    24721 aatggattcc ggtaaaagat ctgcacgcac catgaaaaac ttgctctgct ccttcacatg
    24781 atcaccttcc tatgtctatg tggggcggat tcttttctgc gcagtacgga cattataaca
    24841 ctgcagggtg ggtccggcaa gctgtttcta gctggagacg ggcaattcct gaccggcgat
    24901 atattggctg aacaattcca gttgcggcag gctgcccatc atgataagcg tgtccgtggt
    24961 ttttaggatc tcatggctgt gggggttgat gataatctca ccgtcggcac gtttaatcgt
    25021 caccacagaa caaccggttg cctcccggat accgctgtcg cgaatggcca aaccgttgag
    25081 atggctgtct cccatttgaa acgtgcgtat tttcagaatt tcatcttccg actggggagt
    25141 tttctgatgg cgcatgatct catgataatc cttgaaatac aagtcctcca gataatcaac
    25201 tacattatag gtatcaggct gcagatgcag aatcatatgc cgtaccatct gcaaaccggc
    25261 ttcctcttcc ggttggatga cctcggtagc acccatttcg tatagttttt tcttttccca
    25321 gtaattatgg gcccgactga gaattaacaa ctgattatta tattttttca gcccgttgat
    25381 cacctgttga ttggccaggg tgtccggcaa ggccagcact gccatgcgcg catgagcagc
    25441 gttggcatat tccagcacca tctggctgga agcatcacca tagatatagg gaatccgtgc
    25501 ctgccgtaat ttagcgatgg cggtgtggtc atattcaatc accagaaacg gaatctgcag
    25561 ctgctgcagc gcccggccga cttgtccgcc aacccgaccg tagccgcaga cgatcacgtg
    25621 atcgcagagg gcatttcccg gcaaatctgc caggtcgatt tccggatcag acagaattcg
    25681 ttgcaggaac atgttttgct gaagacggcg gtaaatcccc ggagccaggt taaaaaacag
    25741 cggcgtcaac aagatcgaaa tcaagctgct ggccaggatg acattgtaca ggggagtact
    25801 gatcaactgc tggtcgagac ccattttggc catgacaaag gaaaactctc ccgtctgcaa
    25861 catgcccatg gcggtgtaaa agctaacccg gcttttatat ttgaacaatc ggcttatggt
    25921 ataaaaaaca atgaatttac ccgggatgat cagcagcaaa gcaagcagca gcagcggcag
    25981 gttttggaac aaggtcagcg gattgaccat catgcccacc gaaacgaaaa agaggatgac
    26041 aaaggcatcc cgcagactaa tgatttttcc tagaatttca tgcgcatatt cagattcgct
    26101 gataaacaat cccgccagaa aagcccccag cgaaaccgac aaacccatag cctgggccag
    26161 cactgccaca cccagtccca tgctcagggc caggatcaga aaaatatcgt tgttgctgcc
    26221 ctgcgcgacc ctctccaaaa ccatgggaat aattttgggg gccagataga gcacggcagc
    26281 gatgatcacc gctgccagca caagatcttt gagcagttgg ggaataatct ccggggagaa
    26341 gctctgcaga ctgggcaaca cggaaaccat caggataacg atcaaatcct gggcgatcag
    26401 aatcccgacc atcaactgtc cgtgcagcga gttcatctct ccatggtcag tgaggacgcg
    26461 cagtacgatc atggtactgc tgatggacaa ggtacagcct aaaaacagcg attcataagc
    26521 agacaatccc atccatccgc ctagcaggat ggacaaaccc actaccgcag cgacctgcag
    26581 cagcgctccg cccactgcaa accctttgat ggcttttaac cggctcagag aaaactcgat
    26641 ccccaaggta aacatcaaca ggataacgcc aatatccgca aaaccatgaa ccagttcaat
    26701 attgcatatc aggccggtaa cataagggcc ggctaaaacg ccgcccagca tgtagccaat
    26761 cagcggtgac tgtttcagtg tctcggcgat catcccgcca atcagggcag caataaatat
    26821 gatggcgaca ttaaacaaca tggacgtttc caacaattgc tccccccctg ctttcgccga
    26881 ctcctggtct gagcatatcg gacaattgca ttataacagt ttattctaaa tgcgcacaag
    26941 ccaaggcgat gcctgacttg cgccggcacc gccttgaaat gcttatctgc aaatgtttaa
    27001 atgatgatac agatcagacc gccgctgccc tcattcacaa ttcgctgcag cgtatcctgc
    27061 agtttgtgct gggcattttc aggcatccgg tgcaatttat tctggatgcc ttcccgtacc
    27121 agatcatgca aggatttgcc gaaaatattg gaaccccaga tcttggcggg atcttcttca
    27181 aactcatcca gcatgtaacg caccagttcc tcacattgtt tctccgtgcc gatgatcggt
    27241 gtaatctcgg tggttatatc agcctgaatg atgtgcaggg atggcgcttt ggctttgagt
    27301 ttgacgccaa accgtgaacc ctgtctgatc aattccggtt cttccagaat catctcttcc
    27361 aggtgaggcg tcaccacccc atagccggat tgtttgactt catccagcgc tgccgcgact
    27421 ttatcaaact ctttcttggc aaagctcaga tccttcatca agcggataat gtcgtgattg
    27481 ccttccacgg taaaaccgct gacttcgctg agcgagcgga agaacaaatc ctcggggacg
    27541 gttacctcga tggaggctac accggtaccc agatccattt cctccaaatt aacatagctg
    27601 acattttcga cttcggcaag cttgccaatc gcattctcaa tatcgcgcac tttcttgatt
    27661 ccgctcagaa tctcgcggat ggcatcctcc atatcctgcc gcagccagaa gtcctcttcc
    27721 agttcttcca cccacagcgg caagcgaatg ttcacttcct gtaccgggaa ttcgtagagt
    27781 acttcctcca gtacgccata aatctgttcg acactcatcg tggcacaatc aatcggcatc
    27841 acgctgacat tgaatttttc actcagttct gctgccagtt ccatggtttg cttgtcatat
    27901 ggatgggttg agttcaaaat gacgatgaat ggtttcctca gttcctgcaa ttcactgatg
    27961 acacgctctt cggtctctac ataattgctt ctgggaatgt cgctgatgct gccgtcagtc
    28021 accagtacga tgccaatggt ggagtgatca gcaatgacct tcctggttcc tatttcggcc
    28081 gcttcttcga agggcacctc gtaatcaaac cagggagttc ttaccatgcg gggttcttca
    28141 tcttcttcat accccaaggc tccgcctacg gcataaccaa cacagtcaac cagtctgact
    28201 ttcatgacca gaccgctttg ggtgctgatt tctatggcct cgttgggtac gaatttgggt
    28261 tctgtagtag ttatggtttt gccggcgccg ctctggggca attcgtcttt ggctcgttct
    28321 ctttcataaa cgtcttttat attgggtaaa accatcaatt ccataaatct ttttatgaaa
    28381 gttgacttgc ctgttctgac tggacctact acccccaaat agatatcgcc gcctgttcgc
    28441 tccgcgatat catttaaaat atgattacct tccacttctc atcttccctc cctcggattt
    28501 tgacttttaa ggcacccgtt ttggaaatat ggataccctt gatattgctg ttcaggctat
    28561 aacagtataa atatatttag acagattcgt taatataacc aaaaaaagaa aaaatacgaa
    28621 aaaaaattgg acatccccat taaaggatgt ccacattaca tgccgtctga tttgatgaac
    28681 gcatttaaaa gcaaatacta tggttttgat aattgatatc ttccacctca tgcttgcgct
    28741 ggcgtttcat taattgttcc acttcctcgc gaggcgcccg gttttcataa aggatccggt
    28801 aacaggctct ggtgatgggc atatcgatat tcatctcatt ggccaggtga tgggcaatct
    28861 ggatggtatg ggcgccttcc accaccatgc caataatggc cagtgtttcc tgcaggtttt
    28921 tgccctgtcc gagcagttga ccggcacgtc gattgcggga atgttgactg gaacaggtaa
    28981 ccaccagatc accaatgccg ctcagccccg aaaacgtacg cggatctccg cccatggcga
    29041 cacccattct gatcatttcg gccaggccg
//
LOCUS       DAOTHZ010000023        27852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_1960_length_27852_cov_7.143505, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000023 DAOTHZ010000000
VERSION     DAOTHZ010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 27852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27852
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1960_length_27852_cov_7.143505"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="P5549_04320"
     CDS             complement(<1..1288)
                     /locus_tag="P5549_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRW12326.1"
                     /translation="MDLFSLGSEKMQSDAAPLAWRMRPGNLDEIYGQDHLLAPGSPLR
                     NAIDRDKLHSFVLYGPPGTGKTTIARTIASVTRSHFEYMQAVTAGVSDIRKMAQDARD
                     RHKYYQQKTILFVDEIHRFNKSQQDVLLPFVEDGTLTLIGATTENPLHELNSALLSRM
                     KVYMVHPLDGTALQQIIARVLADKERGLGQVKVTFAEDSLALLIRAAQGDARMILNIL
                     DTLSSSYTDAHGSLLITPALLEKVCGKLIVKYDRSGDWHYDCISAFIKSIRGSDSDAA
                     LYWMAVMLEGGEDPKFIARRLIVHAAEDIGLADPFALSMATAAADAVQYVGLPEARIP
                     MAEAVIYLCTAPKSNRSKLAIDAAIAAVRRQEKIKVPPHLADTSHAQAGRLLGKGIGY
                     KYPHDFGGYVEQSYLPPEIGQPKFYQPGSNGYEKHIQ"
     gene            1468..1935
                     /locus_tag="P5549_04325"
     CDS             1468..1935
                     /locus_tag="P5549_04325"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HRW12327.1"
                     /translation="MKLRNLVKLPLLDGHTAEKLGEIKGAVIGDDFRVLALIMHSQNG
                     FAGCIACDEAIINSDAVLIYDRQCIKPWQDGEQFTVYEAKLGDQVFDEQGRELGTISD
                     FVIDCANQEVWGVEISGGVMQDLLDGRREIPMEKLSWASQENAICDQEGNEQE"
     gene            1932..2108
                     /locus_tag="P5549_04330"
     CDS             1932..2108
                     /locus_tag="P5549_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12328.1"
                     /translation="MTIKCPVCGGLQVGRVGSDQYYCWNCFLEFNFAKGRVNLYEVAE
                     DGSLLAMDESAGML"
     gene            2127..3143
                     /locus_tag="P5549_04335"
     CDS             2127..3143
                     /locus_tag="P5549_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRW12329.1"
                     /translation="MLQSNKLFRYIIFGGVALATFCFAYLVREVVCTFLAGGLMTYFL
                     FRPVALLERHGIPRGWGIAIVYLLLGAITAVLLWIAVPRLLQEINEAAARLPAYAAQL
                     QEFSGRIHDVHLPGKLDLMLQDNLDRIEKSIFALIKDMVNSIYTFFSKVLIIVFAPIL
                     AVYMIKDWEKIKAALLFILPPAARRETVILTSRIDAVWIEYLKGHLLVSALVGLATGL
                     AAVLIGVDFALVIGIIAAITNLVPYFGPILGGIPAILLALVQSWQNGLYMAIAILLIQ
                     QLESNVLTPKIIGDKLGMHPLAVVFALLAGGKLFGIFGMLIAVPLAAALRIIISHAYL
                     KFVE"
     gene            3224..5863
                     /gene="alaS"
                     /locus_tag="P5549_04340"
     CDS             3224..5863
                     /gene="alaS"
                     /locus_tag="P5549_04340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRW12330.1"
                     /translation="MWTSSEIRQSFLQYFENKGHTIVESSSLVPVNDPTLLFTNAGMN
                     QFKDVFLGIDRRNYIRATTSQKCVRAGGKHNDLDTVGRTPRHHTFFEMLGNFSFGDYF
                     KADAIQYAWEYLTEEIKLPKEKLWITIYEDDDEADQLWQQVAGVPAQRIIRMGEKDNF
                     WSMGDTGPCGPCSEIHYDRGTDFSCGHPQCGLGVCDCDRWLEIWNLVFMQYNRDEQGV
                     MTPLPRPSIDTGMGLERISSILQGVQSNYDTDLFVPLIKAVEILTGKPYDRGEGGFAF
                     RVIADHSRACTFLIADGVLPSNDGRGYVLRRILRRAVRFGQSLGISEPFLYKNVDVVC
                     EMMGQAYPQLHEKRDFVKDVIRLEEERFLVTLNEGVKKVADIIKTAQNKGTNAISGEE
                     AFMLYDTYGFPLDLTEDMAEENQFSVDKDGFARMMEEQRQRAKSAHKSENAFARDQLF
                     AELLQEVAPSSFGGYTRFTDESQVLAIFQDDQRVDFAADADIIVVTASTPFYAESGGE
                     VADTGAITAAGGRLVVTDVQKIAGWILHFGSLQGSLRQGDQVQMELDSARRLDISRNH
                     TATHLLHQALRMTLGEHAQQKGSLVEAERLRFDFSHLSALSEEELARIENIVNQAILA
                     MYPVATHATSLEQARDMGATALFGEKYGDEVRVVEVAGFSMELCGGAHVSNTGQIGLF
                     KIVSEGSVGSGLRRIEAMTGRAALQYLNQCENQLKSAAAQLRTVPAEVPGRIESLMQQ
                     LKEKDKEIEAFKAKLSKASVDEIIAQAYAYNGVNILVTATDSTDANHLRQNAERLKDK
                     LAPAVVILAAVVDEKVALAGFAGKEVVARGIHIGKIIGAAAKVTGGGGGGRPDMAQAG
                     GREIAKIEEALATAKKMVENSLA"
     gene            5922..6179
                     /locus_tag="P5549_04345"
     CDS             5922..6179
                     /locus_tag="P5549_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HRW12331.1"
                     /translation="MPQNPSEHTVSFKWEKDDENLVQTILKEVYEALEEKGYNPINQL
                     VGYMISGDPAYITSHRQARNLICKVDRDEIMEVLIKNYLGK"
     gene            6249..7196
                     /locus_tag="P5549_04350"
     CDS             6249..7196
                     /locus_tag="P5549_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRW12332.1"
                     /translation="MLYRPLGRTGLLVSQLCLGVLTIGPLQNHLSLSAGADVLSNGLR
                     AGINFFDTAKIYKTYPYIKKAIKDSGIRPVICSKSYDYTYAGMKQSVEEALDEMGLAC
                     IDIFMLHEQESMLTLKGHRPALDYLLDAKQKGYVKAVGLSTHSVQGVLAGAQSPEIEV
                     IHPLLNIRGLGIMDGSREQMQAAIRYARLKGKGIYAMKVFGGGNLNREAYAACQFVAH
                     NPNIDSLAVGMKSPAEIEVNIAWLEGRRVPELEQNVANQDKRLLIEEWCEGCGQCVEH
                     CRYGALQLNEEGVLEVNQEACLLCGYCARFCPQFCIKIV"
     gene            7209..7658
                     /gene="ruvX"
                     /locus_tag="P5549_04355"
     CDS             7209..7658
                     /gene="ruvX"
                     /locus_tag="P5549_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRW12333.1"
                     /translation="MRIMGLDVGVKRIGIAISDPLGWTAQGHSVLHRTKESKDFAILQ
                     QICRDYEIEAIVLGYPLNMNGTVGPKAEEIKAFGKTLEDHLQLPVSYWDERLSTVSAQ
                     RHLIAADVSRQKRKNVIDKLAAVNILQAYLDRINYKKFDKEDGERIE"
     gene            7685..7972
                     /locus_tag="P5549_04360"
     CDS             7685..7972
                     /locus_tag="P5549_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRW12334.1"
                     /translation="MDHDEIMSDVLVLVDEEGQEYEFEVQAELEMEGETYRVLIPLDV
                     DEDADEEEIEVLILKVVYDEEGNEFMAEIEDEEEWEKVADAWDELVDSGDF"
     gene            8091..9650
                     /locus_tag="P5549_04365"
     CDS             8091..9650
                     /locus_tag="P5549_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRW12335.1"
                     /translation="MNLNTKIFQSRLVQIALILLAMLAFLALYFYNCNYLDDDFRPGT
                     AIAGIAVAGSDRNEARLLLDRQMDQIYNTPVVFAYEDEEEKLELQALLKPVDLDQVVT
                     SAWEADRSQSCYQILTNAVLQRTVNYPLPLVYDSAATDDLLRKWNEKWGTPFHDARLE
                     MDAKQGLIVIPGQIGMTVDRKETFKILPTAMQSIPASISGEIVMARQNPKIGEEALRH
                     MGEIAKYSTRYNPNEVNRTHNLTKATQGINGSIILPGEVFSFNQTVGRRTMETGYKDA
                     MVIVNGKFEPGLGGGICQVSSTLYNASLLAGLEIVERHNHNLTVAYVPLGQDATVSYG
                     TQDFKFRNSTTSPVYVRAAAGGGNLTVNIYGDTHFKQKIEISNIIDQSTPYQTVTLPD
                     NSLQPGETKLDHNGQNGYVVRSFRIFFDQNGEKSKSESLGRSVYIPLHKTILAGPALE
                     EQPPAEGTEPTEGTDPGEGTDPAEGIDPSEEQDPDEGNGSMEEGNQDPAPSPEEPQQP
                     PAEPGQEPTDG"
     gene            9734..10726
                     /gene="mltG"
                     /locus_tag="P5549_04370"
     CDS             9734..10726
                     /gene="mltG"
                     /locus_tag="P5549_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRW12336.1"
                     /translation="MKRVFLVILFFTLLLLGGKLVASQQYQPVDASDQTTVQVKIPDG
                     SSASQVAVLLKNQDLIRSEKAFLKYCRQENLDARLKAGTFNLSRSMSVKEISSQLVTG
                     QNVKDSVTIPEGYTIKEIGQLLEEKKGIQPDEWQQALQDDYDYEFLPPPDRTNRLEGY
                     LFPDTYVFAEADDAHAMIEQMLKNFATRWDKQFAPLMAGTQMDSHEIIIIASLIEREA
                     QKAEERQRIAGVIYNRLDINMPLQIDATVLYSLGAHKSKVNNADLKVDSPYNTYRYPG
                     LPAGPISCPGSASIAAALQPENNNYFYYVSRGDGSHQFSRTFAEHQAAIRKYSQ"
     gene            10830..12053
                     /locus_tag="P5549_04375"
     CDS             10830..12053
                     /locus_tag="P5549_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRW12337.1"
                     /translation="MKQPELLLPAGDLDKLKTALHFGADAVYAGGKEYSLRAAAGNLN
                     QAEMNAGLALLHAKGKKLYVTVNILAHNRDLKNLPAYLESLADMGVDGLIVADPGIMK
                     MARTYAPRIPITVSTQANITNYEAAAVYKEWGASRLVLARELSLQEIRTIKAKVDVEL
                     EIFVHGAMCVSYSGRCLLSHYMTGRSANAGECAHPCRYQYRLVEEKRPEEYYPIEEDE
                     RGSYILNSRDLCLLPYIPQLVQAGIDSFKIEGRMKSALYVAVVAQVYRQALDACRQGA
                     EQFSQAQLHSWMEELQRVATRPFTDGFIAGEVSALQDIAHISPQPRAEFCGIVRGYDQ
                     DRGRIIIEQRANFGPGEDLQLMSTDGQILDLVLEALYDQEGNPLDRARHAKQSVYLPF
                     SREIEAGSILRRVAR"
     gene            12053..12268
                     /locus_tag="P5549_04380"
     CDS             12053..12268
                     /locus_tag="P5549_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HRW12338.1"
                     /translation="MQDQIYARLQPEKIDMLTKLIEAYDHLGIVSTLDQARGLVVIRS
                     TVDCLPELQEVLAHLPFPLELFSTKPE"
     gene            12403..14085
                     /locus_tag="P5549_04385"
     CDS             12403..14085
                     /locus_tag="P5549_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRW12339.1"
                     /translation="MLKRIHFMMGFFLVVFGLLLGKLFFCQIIQGQTLTAQVIKMRSQ
                     SIALREFPRGEILDRNQQSLTRNTGFSSLYCRIQAIPVRDGSRAAGCRELAKWLGTRL
                     QGSDAAEFYERLVRATVQKKLLVKLHEELSQEEIRQLQATPHPALEIVPQWKRYRQDG
                     LLAHVIGYVSAGGEGQAGLEKNYDELLQGGVATQEILLQQDGRGQAIPGINVKVRSTD
                     KATNALGLTIDARVQQAVENIMDQRIARGAVVVMEVHSKEILAMASRPTYNQYRVQEY
                     LADLRSPLLNRTLSSYYPGSVFKIVVSTALVEENLVRQGETFRCSGQYVFNQDLAIPC
                     WKEEGHGEVSFEQAFAGSCNPTFIQLGLRLGKNRLLQYVDQLHLTNPELSGLPGQQKD
                     SYVSIDPGDPAMANACLGQRGVMLTPVQIASLLATIADDGCWSPPQLVKYTLDGSGQM
                     RQLDTVPGEQVIRVATAQKIQSMLAGVVTDGTGQAARLDEVAVAGKTATSQTGRIKEN
                     GEEVLNTWFAGYLPADAPRWVIVVLAEEGKSGAENCAPVFKEIARELLAINP"
     assembly_gap    14188..14287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14317..15858)
                     /locus_tag="P5549_04390"
     CDS             complement(14317..15858)
                     /locus_tag="P5549_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRW12340.1"
                     /translation="MKQTNTDILHQIEEILNIGIALSMEKDHDRLLEMIVYEARRITN
                     ADGGTLYLRDGDLLVFKIIQNQTLHTFKGGKGEPIDLPPVPMRLENVSAYAALRNERI
                     NIPDVYAAAGFDFSGPKNYDQITGYRTVSMLVTPLENHEGEVIGVLQLINALDENNQP
                     IPFSPVYEKVISSLASQAAISLTNQQLIADIEQLFNSFVEVMATAIDSRTPYNANHTR
                     RVALLSGALAAVIEAETEGCWAEVVFDETRTKQLLMSAWLHDIGKIATPLEVMNKCSR
                     IERRLELILQRLDYIWQKTHSEYLEKKLELTSAGEASEVQLKALEDAWAEQQEVIAQS
                     RALIIRADDPATFVDDAIQQELQQIGARRYADPAGQEQPWLTQDELIHLSVTKGTLTD
                     EERIVMEDHVQVTRRMLDKIPFTTKLKDVPFFAGIHHEHLDGKGYPLGLKGEEIPLEG
                     RILALTDVFDALTAADRPYKKALSIGQSLKILGFMVKDGELDGDLLDLFVKHRVWEMI
                     ASDEA"
     gene            complement(15855..16721)
                     /locus_tag="P5549_04395"
     CDS             complement(15855..16721)
                     /locus_tag="P5549_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12341.1"
                     /translation="MVKEVEAMTQPDFKVVFWGVRGSIPVPGMQTVRYGGNTSCVQIQ
                     IGSRLLIMDAGTGIHKLGQALLQANQPINGDIFISHTHWDHIQGFPFFAPAFIKGNAF
                     NLYGQTKVNLNFGDLMRGQMMYEHFPVSLEQMGATITMNELISGMTLDLEDGIQVRTL
                     ESNHPGGCLCYRVDHAGRSCCYMTDMEHSPQMDPAMIAFAAQADVLIYDANFTDDEYN
                     GVGGLPSRVGWGHSTWQAGIKLLEAARARQLILFHHSNFHDDDDMDRIEQDARRQYAQ
                     VTAAREGMVISL"
     gene            complement(16735..17166)
                     /locus_tag="P5549_04400"
     CDS             complement(16735..17166)
                     /locus_tag="P5549_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRW12342.1"
                     /translation="MQDFTTFICYRLKSTMKRVEKHIGSTLENYGVTMAQSFILFSLL
                     EKDGCTLTEIGNRAQIDNSSLTTMVDKLEKEGLVERRLFAQDRRVVNLYLTEKGRELG
                     KKVYDSGAAMNAELRTLLNSGEEDFYSNLEGISRILDQKKD"
     gene            17423..18121
                     /gene="sigK"
                     /locus_tag="P5549_04405"
     CDS             17423..18121
                     /gene="sigK"
                     /locus_tag="P5549_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HRW12343.1"
                     /translation="MSVLYWALAIAAVVKGSLLLFGYLNNQVFPQPLDAEEEKRYLDE
                     LQNGSEEARNKLIEHNLRLVAHVVRKYESSGEELEDLISIGIIGLIKAIKTYNHERGV
                     RLATYAARCIENEVLMHLRNIKKLKQEISIYDPIGYDKEGNEISLIDILTSDSEIIEQ
                     IDIRMQEEKVKNNLGVLNRRERQVIEMRYGVFSGLKETQRDIAKKLGISRSYVSRIEK
                     RAVNKLMKEISCQG"
     gene            complement(18220..18597)
                     /locus_tag="P5549_04410"
     CDS             complement(18220..18597)
                     /locus_tag="P5549_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRW12344.1"
                     /translation="MALRTIVKIDEELCNGCGVCISPCAEGALALINGKARVIKEELC
                     DGAGFCLGVCPTGALSLEQREAPDFSEEAVHAHKQGIKINYIPQECSFCGISEDDAYV
                     MAVKHKGQALWVCTRCLPRLIHG"
     gene            complement(18683..19030)
                     /locus_tag="P5549_04415"
     CDS             complement(18683..19030)
                     /locus_tag="P5549_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRW12345.1"
                     /translation="MDEKIIVGADKEILPQVANSAASVEYQKDAVVSKTLINKSAGTV
                     TLFAFDQGQGLSEHTAPFDAMVYLLDGEAEIIISGHPYIVKQGESIIMPAHHPHALKA
                     VQPFKMMLIMIKS"
     gene            complement(19061..20689)
                     /gene="hcp"
                     /locus_tag="P5549_04420"
                     /gene_synonym="priS"
     CDS             complement(19061..20689)
                     /gene="hcp"
                     /locus_tag="P5549_04420"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRW12346.1"
                     /translation="MFCNQCEQTSKGIACTVVGVCGKKPDVAALQDLLLYAAKGLSIY
                     AAEARKHGIVDGKVNRFTMKVLFSTLTNVNFDPESFAEMLSECVALRTQLQEKMAAAG
                     HTPASTDPSVKFVPAGSVAELVSQGQEIGFLVDLNGDADLVSLQELLIYGLKGVAAYA
                     YHASILNQEDDAVYAFTQEALAQIGNPALGLNDWLGLVLQCGAINLRTMELLDAGNTG
                     SFGHPVPTEVPLGHKAGKCILVSGHDLKDLEELLEVTRDSGIYVYTHGEMLPAHGYPE
                     LKKYPHLYGNYGTAWQNQKKEFANFPGAILMTTNCIQEPQDTYRENIFTTGPVGWPGV
                     RYVQNGDFAAVIAKAQEMPGFSSDEDLGSVMVGFAHNAVMSVADQIIGAVKSGDLKHI
                     FLVGGCDGAKSGRNYFTDFVEQTPDNTAVLTLGCGKYRFFDKQLGSIGGIPRLLDVGQ
                     CNDAYSAVQIAVALSEAFDCGVNDLPLSMVISWYEQKAVAVLLTLLYLGIKDIRLGPS
                     LPAFVSPNVLDVLVKNFDLKPIATPEQDLAAILG"
     gene            complement(20774..21223)
                     /locus_tag="P5549_04425"
     CDS             complement(20774..21223)
                     /locus_tag="P5549_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRW12347.1"
                     /translation="MQLTRQCEYALRTMLELAAHPYGELLSTKIISERQDIPEDFLKK
                     TIHLLAVNGLVSTVRGTQGGVRLARPAQDIKVRDVVEAIEGPIAINPCLASGFICPNQ
                     PRCRISPVLARAQQAFVAELSKESIADLAAGDFDSAYDSSSDLPSEE"
     gene            21439..22455
                     /gene="galT"
                     /locus_tag="P5549_04430"
     CDS             21439..22455
                     /gene="galT"
                     /locus_tag="P5549_04430"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRW12348.1"
                     /translation="MKEEEKMSELRRDLVKNNWVSVAEHRALKPNDFPIAKKALESSA
                     TGGFCPFCEGNEPFTPPEIAACRQGSEPNTKGWQVRAIPNKFSAFELEGELEETRLGV
                     FSRYNGLGKHEVLIETPEHNVDFHQLSVERMETIIKMLRDRYNALAVDPRIKYIQIYK
                     NRGIFAGASLEHSHSQIVALPFVPSENQGVIDYYQANRRCLLCDIADQERHGERLVAE
                     SEHFILLCPYAPRFPYETWIIPQQHRAHFGDIKDEEIHDLAQIIKKFCNVMIESLSDP
                     SYNLVINTAPVDVSDQPGYHWYIEVTPRLLVNAAVEIASGVYINPVAPELAAAMLREK
                     MNPV"
     gene            22477..23004
                     /locus_tag="P5549_04435"
     CDS             22477..23004
                     /locus_tag="P5549_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HRW12349.1"
                     /translation="MTNKLYPRMYVDTLLDVPLEELAALGVRAFILDLDNTITEWNSQ
                     ELRAEVEAWFRTIRERGFKACILSNNGEQRIVQIAARLEIPFIHRARKPMRGAFLQAV
                     EIMGMQPDAVAVIGDQIFTDILGGNRAGLYTILVKPLARREFYGTKISRAMEFFVLRR
                     YRRRSKLANNGRPGL"
     gene            23095..23970
                     /gene="aroE"
                     /locus_tag="P5549_04440"
     CDS             23095..23970
                     /gene="aroE"
                     /locus_tag="P5549_04440"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRW12350.1"
                     /translation="MQKSADILLGLLGNPVEHSLSPLMQNSAIRWLGLPYVYLPFCIE
                     PEHLPAAVAAIRALSMGGVNVTIPFKEAVIPYLDALSEDARLCGAVNLIHNQGGKLLG
                     LNTDGEGFIRSLREEDLHPQGQFVLIGAGGAARAIAGAISQFPIEQIVFLDIDPARAH
                     HLAGHIAQQTGCCCQAAVMNRENFAAASAQADFIVNCSSVGMFPHIEGCPVDTFAQAK
                     DSAVVYDLIYNPLQTRFLQLASTRGLKTVSGLSMLVHQGALSLEILTGCQAPVSHMKA
                     VVSDYYAQQQRIYAG"
     gene            23939..24403
                     /locus_tag="P5549_04445"
     CDS             23939..24403
                     /locus_tag="P5549_04445"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HRW12351.1"
                     /translation="MHNNKGFTLAELIFCLALLTILMAVAMPHFSTALTELKLTADAR
                     QMAGVLRMARTEAITTGTNRQVVFYVNDSQRPRYQSVGRASYPLSPGVKFAGTKPFNS
                     RFKNQPACIFMPNGRAVQGGTVTLKADQKYKYVIVLNTTGRIRVSDTPPESW"
     gene            24422..24931
                     /locus_tag="P5549_04450"
     CDS             24422..24931
                     /locus_tag="P5549_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRW12352.1"
                     /translation="MMILQLLFIFFARIIDVSLGTIRTILIIRGEKWPAAIIGFFEIM
                     VYTVALGMVVGSLDDPLRLLVFCTGFAMGVLVGSIIEEKLALGFRGLQVTIDLENAHI
                     VDELRDEGYPVTCWQAQGKLGPKLVLNIITKRNLAQKITDQICACDPNAFVVYMEPKF
                     FQGGYIKKK"
     gene            24986..25546
                     /locus_tag="P5549_04455"
     CDS             24986..25546
                     /locus_tag="P5549_04455"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRW12353.1"
                     /translation="MRKNIVLIGFMGTGKSSIGVRLASKLNAEFIDMDREIETLNGMS
                     VSEIFRRYGERRFRSEETLMARKLSAREGLIIATGGGAVLNPENIAIFRQNSIIILLE
                     ASAEDIWTRVNRKKGTRPLLKGASNVADIEKLLAVRAPYYDCADIRVNTSDKELEFII
                     REILQKIKEVSDPGGDNYQNALNRPQ"
     gene            25631..27328
                     /locus_tag="P5549_04460"
     CDS             25631..27328
                     /locus_tag="P5549_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRW12354.1"
                     /translation="MSKRKMIGEILVDMGLITQEQQQSVLEEQQKTGDKFGNIMLRKG
                     LLTEQQLLETLEFSLGIPQVQLSKMDIDPEAVKLLPPQLIRLHQVLPIARRKNILTLA
                     MADPLNQQAIDDVRLATNMDVVPVLASERDMDTAIRQYLAFRLDPGMENIVNELGLDD
                     KASAMRLKELQNMKLDEDAPIIRMVNSIMVQAVQGRCSDVHIEPQAKDLRVRFRVDGE
                     LYEVLTLPQLSQAAVLSRIKIMANMDIAEKRIPQDGRFRMVVDGREVDFRVSTLPTAL
                     GEKAVMRILDRNNALTRVDQLGLSQRNKEYLLSLSRRPHGMLLVTGPTGSGKTTTLYS
                     VLGEVNSVDKNIITLEDPIEYTLAGINQVQTNPRAGLTFASGLRSILRQDPDIIMVGE
                     IRDQETAQLAVQAALTGHLVLSTLHTNSAAGTIARIEDMGIEDFLLSSSLSGIVSQRL
                     VRQLCPNCKQKYILEEETACKLGMPEESGQTFYRSVGCNMCRQLGYQGRIALHEVMVV
                     GPKVRQLINKGENSEDEIAVAAIKEGMITLRDDGLEKARQGKTSLEEVMKVVLLEGK"
     gene            27332..>27852
                     /locus_tag="P5549_04465"
     CDS             27332..>27852
                     /locus_tag="P5549_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRW12355.1"
                     /translation="MQTNQNIMDIVKNGVELGASDIHLTVFRPPIYRVNGKLFSLPED
                     KVLDGEDTIRLATELFPNEKILPTLQETGQVDFSNSMPGVGRFRVNTFIQRGSWACAI
                     RLIPMHVPRLETLGLPPAISELAQKEKGLILVTGVTGSGKSTTLAAMVDYMNQHKNLN
                     ILTLEDPIEYLHR"
BASE COUNT         6865 a         7009 c         7537 g         6341 t
ORIGIN      
        1 cctgaatatg cttttcataa ccgttgctgc cgggctgata aaatttgggc tggcctattt
       61 ccggcggcag gtagctttgc tctacgtaac cgccaaagtc atgcggatat ttatagccga
      121 ttcctttacc cagcaaccga ccggcctggg catgggaagt gtcagccaga tggggcggca
      181 ctttgatctt ttcctgccgg cggacagcgg caatggctgc atcaatcgcc agtttgctgc
      241 ggttgctttt cggggcggta cacaggtaga tgacggcctc ggccatgggg atgcgggctt
      301 ccggcaaacc gacgtattgc accgcatcgg cagctgccgt agccatggac agggcaaaag
      361 gatcggccag accgatatcc tcggcggcat gcacgattaa acgccgggca ataaatttgg
      421 gatcctcgcc gccttccaac atgaccgcca tccagtataa agccgcatcc gaatccgagc
      481 cgcgaatact ttttataaat gcggaaatgc aatcgtaatg ccagtcgccg ctgcgatcat
      541 acttgacgat caacttgccg cagacttttt ccagcaaggc cggggtgatc agcaatgagc
      601 cgtgggcgtc ggtataggag ctgctcagcg tgtcgagtat attcaggatc atgcgggcat
      661 ccccctgggc cgcccggatc agcaacgcca gactgtcttc ggcaaaggtt acctttacct
      721 gtcccagtcc ccgttccttg tcggccagca ccctggcaat gatttgctgc aaagcagtgc
      781 catccagagg atgcaccatg tacaccttca ttcgcgacag cagcgctgaa ttcagttcat
      841 gcagagggtt ttccgtggtg gctccgatca gggtcagggt accgtcttcc acaaagggaa
      901 gcagcacgtc ctgttgggat ttattgaaac gatggatttc atccacaaaa agaatggttt
      961 tttgttgata gtatttgtgc cggtccctgg catcctgggc cattttgcgg atatcgctga
     1021 cgccggcggt taccgcttgc atgtattcaa aatgagacct ggtcacactg gcaatcgtac
     1081 gggcgatggt cgtcttgccg gtgccggggg gaccatagag gacaaaggag tgaagtttgt
     1141 cgcgatcgat tgcattgcgc aaaggactgc ccggcgccag caaatgatcc tgcccgtaaa
     1201 tctcatccag atttccgggt ctcatccgcc aggccagcgg cgcagcatca gactgcattt
     1261 tttcgctgcc cagactaaac agatccatct cgcatgcctc cctgccgatt acttccagcg
     1321 tttgctgcga tcattataga acatttgttt atttcctgca agtgcacgct tcgggatgtg
     1381 tcctccaggt gggtattttt tatcgcagtt ggggtaaaaa tagcatcagc aatatatgcg
     1441 atggctcaac caggaaggat ttatgctttg aagctgcgca atctggtcaa acttcccctg
     1501 ctcgatggac ataccgctga aaagctgggc gaaatcaagg gcgctgtcat cggcgatgat
     1561 tttcgcgttc tggcgctgat catgcacagt caaaacggtt tcgccggctg tattgcttgt
     1621 gacgaagcca ttatcaattc ggatgccgtg cttatttatg accggcaatg tattaaaccc
     1681 tggcaggatg gggaacaatt cactgtatat gaagccaaac tgggagatca ggtttttgat
     1741 gaacaaggac gggaattggg cacgatcagc gatttcgtca ttgattgcgc caaccaggaa
     1801 gtttggggtg tagaaatctc agggggcgtt atgcaggacc tgttggacgg gcgccgggag
     1861 atcccgatgg aaaaactgag ttgggccagt caggaaaatg caatctgtga tcaggaaggg
     1921 aatgagcagg aatgaccata aaatgtccgg tttgcggagg tctgcaggta ggcagggtag
     1981 gcagcgacca gtattactgc tggaattgtt tcctggaatt caattttgcc aaaggccggg
     2041 tcaatctcta tgaagttgct gaagacggca gtctgctggc catggatgag tccgccggca
     2101 tgttatagag aggcgtgaaa taaagagtgc ttcagtccaa caagctgttt cgatatatta
     2161 tttttggcgg ggtggcattg gccacctttt gttttgctta tctggtcaga gaagtggtct
     2221 gcacgttcct ggccgggggc ttgatgacgt atttcctgtt tcgcccggtt gcattgctgg
     2281 agcgtcacgg gataccaaga ggttggggga ttgccattgt gtatctgctg ctgggcgcca
     2341 ttacggcggt tttgctgtgg atcgcagtgc cccgcttgct gcaggaaata aacgaagccg
     2401 ctgccaggct gccggcttac gctgcccaat tgcaggaatt ttccgggcgg attcatgacg
     2461 tacatttacc cggcaagctg gacctgatgc tgcaggacaa cctggaccgg atcgaaaaat
     2521 ccatctttgc cctgatcaaa gacatggtca acagcattta cacctttttc agcaaagttt
     2581 taatcattgt atttgcaccc atcctggcgg tttacatgat caaagactgg gaaaaaatca
     2641 aagctgcctt gttatttatt ttgcctccgg ccgcgaggcg ggaaaccgtt attctgacca
     2701 gccgcatcga tgcagtttgg atcgaatatt tgaaggggca tctgctggtg tctgcgctgg
     2761 tcgggctggc caccggtctt gctgccgtct tgatcggcgt tgattttgct ctggtgatcg
     2821 ggattatcgc cgcaatcacc aatctggtgc cctatttcgg gccgattctg ggcgggatac
     2881 cggccatcct gctggcgttg gtccagtcct ggcagaacgg actttacatg gccatcgcca
     2941 tccttctcat ccaacaactg gagtccaatg tattaacgcc caagatcatc ggggacaaac
     3001 tgggtatgca tccgctggca gtggtttttg ccttgctggc gggcggaaaa ctgttcggca
     3061 ttttcggtat gctgatcgcg gtaccgctgg cagcagcact gcgcatcatc atttcccacg
     3121 cgtatttaaa gttcgttgaa tagcaagttt attgacatcc ctcggcgaat ctatgaaaat
     3181 agtatagtta ggaagaattc aagggaaaga ggaggccaat ctggtgtgga cgagcagtga
     3241 aatccgtcaa agtttcttgc aatactttga gaataaaggt catacgatcg tggaaagttc
     3301 ttccctggtg ccggtgaatg atcccacgtt gctttttacc aatgccggga tgaatcaatt
     3361 caaggatgtt ttcctgggaa tcgatcggcg caattatata cgagccacga cttcgcaaaa
     3421 atgtgtgcgg gcgggcggca aacacaatga tcttgacacg gtggggcgga ccccgcggca
     3481 tcataccttc tttgaaatgc tgggcaattt ttcatttggg gattatttta aagcggatgc
     3541 catccaatac gcttgggaat atctgaccga agaaatcaaa ctgccgaaag aaaaattatg
     3601 gatcaccatc tacgaagacg atgatgaagc cgatcaattg tggcagcagg ttgcgggtgt
     3661 ccccgcgcag cggattatca ggatgggaga aaaagataat ttctggagca tgggcgacac
     3721 cggtccctgt ggtccctgca gtgaaattca ttatgaccgg ggcacggact tctcctgcgg
     3781 acatccgcaa tgcggactgg gggtttgcga ttgtgaccgc tggctggaga tctggaatct
     3841 ggttttcatg cagtacaatc gtgacgaaca gggcgtcatg acgcctttgc ccagacccag
     3901 tattgatacc ggtatgggat tggagagaat cagctcgatt ttacagggag tacaaagcaa
     3961 ttatgatacg gatttgtttg tgccactgat caaagcggtt gaaatcttga ccggaaagcc
     4021 gtatgaccgg ggtgaaggcg gctttgcctt ccgcgtcatc gccgaccaca gccgcgcctg
     4081 tacttttttg atcgctgatg gggtgctgcc cagcaatgac ggacgggggt atgtactgcg
     4141 ccggattctg cgccgggcag ttcgctttgg ccagtcgctg ggaatcagtg agccgttttt
     4201 atataaaaat gtagatgtag tttgcgaaat gatgggacag gcctatcctc aacttcatga
     4261 aaaacgcgat tttgtcaagg atgtcattcg cctggaagaa gaacgctttt tagtaacctt
     4321 gaatgaaggg gtaaaaaaag ttgccgatat tatcaaaaca gctcaaaaca aggggacgaa
     4381 tgccatcagc ggcgaagaag cctttatgct gtatgatact tacggatttc cgcttgacct
     4441 cactgaggat atggccgaag agaatcaatt cagcgttgac aaggacggct ttgcccgcat
     4501 gatggaagaa cagcggcagc gggccaaatc agcgcacaag agtgaaaacg cctttgcccg
     4561 ggatcagctc tttgccgagc ttttacaaga agtagccccc agcagttttg gcggttatac
     4621 cagatttact gatgagtccc aagtgctggc cattttccaa gatgatcagc gggttgattt
     4681 tgctgccgat gcagatatta tcgtggtcac cgccagtacc cctttctatg cggaaagcgg
     4741 gggagaagtg gctgataccg gtgccattac tgctgccggt ggtcgcctgg tggtcacgga
     4801 tgtgcaaaag atcgccggtt ggattttaca ttttgggagc ctgcaaggca gtttgcgcca
     4861 aggtgaccag gtgcagatgg aactcgattc cgcccgtcgc ctggacattt cccgcaatca
     4921 tacggcaacc caccttttac accaggcgtt gcgcatgacg ctgggggaac atgcccagca
     4981 aaaaggatcg ctggtcgagg cggaacggct gcgctttgat ttttcgcatt taagtgctct
     5041 cagcgaggaa gaactggccc gtatcgaaaa catcgtcaac caggcgattt tggccatgta
     5101 tcccgtcgcc acccatgcaa ccagtcttga acaggcgcgg gacatgggcg ccaccgcctt
     5161 gtttggagaa aaatacggag acgaagtgag agtggtggaa gtagccggat tcagcatgga
     5221 gctttgcgga ggtgcgcatg tgtcaaacac tggccaaatc gggcttttca agattgtgag
     5281 cgagggcagt gtcggttcgg gattaagacg tattgaagcc atgaccggca gagcagccct
     5341 gcaatatctg aaccaatgtg agaatcaact aaaatcagcg gcagcccagt tgcgcaccgt
     5401 accggcagaa gtacccggcc gaattgaaag cctgatgcaa cagctaaaag agaaagacaa
     5461 agagatagaa gcattcaaag caaaactatc caaggcttcg gtcgatgaaa tcatcgccca
     5521 ggcctacgca tacaacggtg tcaacattct ggtcactgcc acggacagca ctgacgccaa
     5581 tcatctccgc cagaatgcgg aacggctgaa agacaaactc gctccggcag ttgttatcct
     5641 ggcagctgtt gtggatgaaa aggtggcgct ggccgggttt gccggcaagg aagtggtggc
     5701 ccgcgggatt catatcggta aaataattgg agctgcagcc aaggtaaccg gcggcggcgg
     5761 aggcggccga cccgacatgg cccaggccgg cggcagggag attgccaaaa tcgaggaagc
     5821 tctggcaacc gccaaaaaaa tggtagaaaa ttcattggct taaacagagg gtttgccaat
     5881 gcggatgtcg aaataggttt agagcaaggg ggggagcagc gatgcctcag aatccaagcg
     5941 aacataccgt cagtttcaaa tgggaaaaag atgatgagaa tctggtacag acgattctaa
     6001 aagaggttta tgaagcgctg gaggaaaaag gatataatcc gattaatcaa ctggttggtt
     6061 atatgatttc cggagatccc gcttacataa ccagtcatcg gcaagcccgt aacctaatct
     6121 gcaaagtcga tcgcgacgag attatggaag tactgattaa aaactatctg ggtaaataag
     6181 ttgtgcagca aaaaggtgaa aacgaggggt tttcaccttt tgtttccatg tccaaatgag
     6241 gtgttgattt gctatataga ccactgggca gaaccggtct gctggtttcg cagctgtgcc
     6301 tgggggtgct caccatcggg cccctccaga accacctgtc gttgtcggcg ggtgctgacg
     6361 tattgagtaa tggtttgcga gccggcatca acttttttga taccgccaaa atttacaaga
     6421 cctatcccta catcaaaaag gcgatcaaag acagcgggat caggccggtc atctgcagca
     6481 aatcctatga ttatacctat gcggggatga agcaaagtgt agaggaagcc ctggacgaaa
     6541 tgggccttgc ctgcattgat atctttatgc tgcatgaaca ggaaagcatg cttaccctca
     6601 aaggccatcg gccggcgctg gattatctgc tggatgccaa acaaaaaggc tatgttaaag
     6661 cagtcggact atccacgcat tccgtacagg gggtcctggc cggtgctcaa tcaccggaga
     6721 tcgaagtcat acatcccttg ctcaatattc ggggcctggg catcatggac ggttcccggg
     6781 agcaaatgca ggccgccatc cgctatgcca gattgaaggg caaaggcatt tacgccatga
     6841 aagtcttcgg tggcggcaac ctcaaccgcg aggcctatgc tgcttgccaa tttgtcgccc
     6901 ataatcctaa tatagacagc ctggccgtgg gcatgaaaag ccccgccgaa atcgaggtca
     6961 acatcgcttg gctggaagga cgccgcgttc cggagctgga acaaaacgtg gccaaccagg
     7021 acaagcggct gctgatcgaa gaatggtgtg aaggctgcgg ccaatgtgta gagcattgtc
     7081 gttatggcgc gttgcaattg aatgaggagg gcgttctgga agtgaatcaa gaagcttgtc
     7141 tgctgtgcgg ttattgtgca agattttgcc cgcaattctg tattaaaata gtatagggaa
     7201 gggtttttat gagaataatg ggcttagacg taggcgtgaa acggattggc attgccatca
     7261 gcgacccttt gggatggacg gcgcaggggc attcggtttt gcaccgtacc aaggaaagca
     7321 aggatttcgc gatcctgcag cagatttgca gggattacga aatagaagcc atcgttctag
     7381 gttatccgct gaatatgaac gggacggttg gccccaaagc ggaagagatc aaagcattcg
     7441 ggaagacgtt ggaagatcat ttgcagttgc ccgtttccta ttgggatgag cgtttgtcaa
     7501 ccgtaagtgc ccagcgtcac ttgatcgccg ccgatgtatc ccgccaaaaa cgcaaaaacg
     7561 taattgacaa gcttgccgcg gtaaacattt tgcaggccta tttggatcgg attaactaca
     7621 agaagtttga caaagaagac ggtgaaagga tagaatagcc ttagtttttg gaaggggtga
     7681 aacgatggat cacgatgaaa ttatgagcga tgttctggtg cttgtcgatg aggaaggaca
     7741 ggaatacgaa ttcgaagtac aggctgaact ggagatggaa ggagaaacct atcgggtttt
     7801 gattccgctc gacgtagatg aggacgcaga tgaagaagaa atcgaagtgc tgatcctcaa
     7861 agtggtgtat gacgaagaag gcaatgaatt tatggcggaa attgaagacg aagaagaatg
     7921 ggaaaaggtc gccgacgcat gggacgagtt ggtagacagc ggagacttct aagtcagtag
     7981 aaaagaagcc gccaggcttc tttttatgct ttcccaggaa tacaattgcc gcctgatcag
     8041 taaatataaa gcaggtcggg agcgtataga aaattgttgg gggtctgagt ttgaatctga
     8101 atacgaaaat tttccaatca aggctagtgc aaatagcctt gattctgctt gccatgttgg
     8161 cgtttctggc gctttatttt tataactgca attatctgga tgatgacttc agacctggca
     8221 ccgccattgc cggtatcgct gtggccggtt ccgatcggaa tgaagccagg cttttgcttg
     8281 accggcaaat ggatcaaatc tacaatactc cggtagtgtt tgcctatgag gacgaagagg
     8341 aaaaactcga actgcaagcc ttgctcaaac cagtggacct tgatcaagtg gtcacttccg
     8401 cctgggaagc cgaccgttcc caaagctgct accaaatcct caccaacgca gtcctgcaac
     8461 gcaccgttaa ttatcccttg ccgctggtct atgattctgc agcgacagac gacttgctga
     8521 gaaaatggaa tgaaaaatgg ggcactcctt ttcatgatgc ccggttggaa atggacgcca
     8581 aacaaggtct gatcgtgatt cccggtcaaa ttggcatgac cgtcgatcgc aaagagacct
     8641 ttaagatcct gccgaccgcg atgcaatcca ttccggccag tatcagcgga gaaattgtca
     8701 tggcgcggca aaaccccaag atcggtgaag aagccctgcg acatatgggc gaaatcgcca
     8761 aatattcgac ccgatacaat cctaatgaag tcaaccgcac ccataatctg accaaagcca
     8821 cccagggcat caacggcagc atcatcttgc cgggagaggt tttctcgttt aaccagacgg
     8881 ttggacgccg gacgatggaa accggctaca aggatgccat ggtgattgtc aatggcaaat
     8941 tcgaacccgg cttgggcgga ggcatctgcc aggtttcttc aaccctttat aatgccagtt
     9001 tgttggcggg attggagatt gtggagcgtc acaatcataa cctgacggtc gcctacgtgc
     9061 cgcttgggca ggatgcgact gtatcctatg gaacccagga ctttaaattc aggaacagca
     9121 ccacttcccc ggtttatgtg cgggccgctg cggggggagg aaatctgacc gtaaatattt
     9181 acggggatac ccactttaaa cagaaaattg agatatccaa tattatcgat cagtcgactc
     9241 cttatcagac ggttaccctg ccagacaaca gcctgcagcc aggggaaacc aaactcgatc
     9301 ataacggtca gaacggctat gtggtcagat cattccggat tttttttgat caaaacgggg
     9361 aaaagagcaa gagtgaatcg ctgggacgca gcgtttatat tccattgcac aagaccatcc
     9421 tggccggacc cgcgctggag gagcagccgc cggcggaggg gactgagccg acagaaggca
     9481 ctgacccggg cgaaggaacc gatccggctg agggaatcga tccgtctgaa gagcaagacc
     9541 cggacgaagg taacggatct atggaagaag gaaatcaaga cccagcgccg tccccggaag
     9601 agccgcagca acctccagca gagccgggac aagaaccgac ggatgggtag gcgatcattg
     9661 ctgatcgaga tgaatcaacc gaagacaggt gaaaaggatg cgtgaaacgc cattgcaacg
     9721 cgggaagatc agggtgaaaa gagtcttttt ggtgatcttg tttttcactt tgctgttgct
     9781 gggcgggaag cttgttgcca gccagcaata ccagcccgtg gatgcaagcg atcaaaccac
     9841 agtacaagtg aaaattcctg atggcagcag tgccagccag gtagccgttt tactgaagaa
     9901 ccaggattta atccgcagtg aaaaagcatt tttgaaatac tgccgccagg aaaacctgga
     9961 tgcgcggctc aaagccggaa cattcaactt gagccgcagc atgagcgtca aggaaatcag
    10021 cagtcagctg gtaaccgggc agaacgtgaa ggattcggtc accattcccg aaggctatac
    10081 catcaaagaa atcggccaac ttttggaaga aaagaagggc atacagccgg atgaatggca
    10141 gcaggctttg caggatgatt atgattatga gtttctgcct ccgccggacc ggaccaaccg
    10201 cttggagggc tatctgtttc ccgatacata tgtattcgcc gaagcggatg atgcccatgc
    10261 catgattgag cagatgctga aaaacttcgc gacacgctgg gacaagcaat ttgctccgct
    10321 gatggcgggg acgcagatgg attcgcatga aatcatcatc attgcctccc tcattgagcg
    10381 ggaagcacag aaagccgagg agcgccagcg cattgccggc gtgatctaca atcgactgga
    10441 catcaatatg ccgctgcaga ttgacgcgac cgtactctac agcctggggg cgcataagag
    10501 caaagtcaat aatgctgatc tgaaagtgga ttcaccctat aacacttacc gttatcccgg
    10561 actgccggcc ggccccatct cctgcccggg cagtgcctcg attgcggcag ccctgcagcc
    10621 ggagaataac aactattttt actatgtctc ccggggagat ggcagccacc aattctccag
    10681 gacattcgcc gaacatcagg cggccattcg caagtattcc cagtaaatca acccaccatt
    10741 gcgcaagtca agaaccgtcc ccaacttgcg caagttgggg acggttcttg acttgcgcga
    10801 ataagctcag taataaagga aatgcaacca tgaaacaacc cgaactgctc ctgccggcag
    10861 gggatctgga caaattaaaa acagcgctgc actttggtgc tgatgcggtc tatgcgggcg
    10921 gcaaggaata cagcctgcgg gcagccgccg gcaacctaaa ccaggcggaa atgaatgcgg
    10981 gcctggcgct gttgcatgcc aaaggtaaaa aactctatgt aaccgttaat attctggccc
    11041 ataaccggga tttgaaaaat ctgccggcct atctggaatc cctggcggat atgggggtgg
    11101 acggattgat tgttgctgat cccggcatta tgaaaatggc ccgcacatat gctccccgca
    11161 ttcccattac cgtaagcacc caggccaata ttaccaacta tgaggcggcc gctgtttaca
    11221 aagaatgggg agccagccgg ctggtgctgg cgcgggaatt atcgctgcaa gagatccgga
    11281 ccatcaaagc caaagtggat gtggaactgg aaatttttgt gcatggggcc atgtgcgtgt
    11341 cctattccgg gcgctgtctg ctgtcccatt atatgaccgg acgcagtgcc aacgccgggg
    11401 aatgtgccca tccctgccgc taccagtatc gtctggtgga agaaaaacga ccagaggaat
    11461 attatcccat tgaagaagat gagcgcggca gttacatcct gaactcccgt gatctgtgcc
    11521 tgctcccgta cattccgcag ttggtgcagg ccgggattga ttcgttcaag attgaaggcc
    11581 gcatgaagag tgccctctat gtagccgtgg tggcacaggt ttaccgccag gccctggatg
    11641 cctgccggca aggcgctgaa caattcagcc aggcccagct gcacagctgg atggaagaat
    11701 tgcagagggt cgccacaaga ccctttaccg acggttttat tgccggcgaa gtctctgccc
    11761 tgcaggacat cgcccacatt tctccgcagc cgcgggcgga attctgtggg attgtcagag
    11821 gttacgatca ggaccgcggc agaataatca tcgagcagcg tgccaatttc ggcccgggag
    11881 aggatttgca gctcatgtcc accgatggac agattctgga tctggtgctg gaggccttat
    11941 atgaccagga aggcaatccc cttgaccgtg cccgtcacgc caaacaatcg gtctatttgc
    12001 ctttttcccg cgaaattgaa gctggttcca tcttgcgaag ggtggcccgt tgatgcagga
    12061 ccagatctat gcccggctgc agccggaaaa aattgatatg ctgaccaaac tgattgaagc
    12121 ctatgatcat ctgggaatcg tttccacgct tgaccaggcc cggggactgg tcgtaatccg
    12181 ttcaaccgtc gactgcctgc cggaattgca ggaagtcctg gcccatcttc cttttcccct
    12241 cgaattgttt tccaccaaac cagaataaac caagcctgcc cagacttgcc caacccatcc
    12301 cgacgcagct gccgggtgct tttctgcggg tataagccgg ccgctttctg cccaagctaa
    12361 agtaaacgaa atccatctgt gtgttgaacg ggaggcggcg gcttgcttaa gagaatccat
    12421 ttcatgatgg gatttttcct cgttgttttt ggcttgctgc tgggcaaact gtttttctgc
    12481 cagatcatcc agggacaaac cctgaccgct caagttatca agatgcgcag ccaatcgata
    12541 gccctgcgtg agtttccgcg cggcgagatc ctggatcgca atcaacaatc cctgacccgg
    12601 aatacgggtt tctccagcct ttattgccgt atacaggcca tccccgtgcg cgacgggtca
    12661 cgggcagcag gttgccgcga attggcaaaa tggctgggca cacgtttgca gggcagcgat
    12721 gcagcggaat tctatgaacg gctggtcaga gcgactgtgc aaaaaaagct tttggtcaag
    12781 ttgcatgagg agctcagcca ggaggaaatc aggcaactgc aggccacgcc ccatccggcg
    12841 ctggaaatcg ttccccaatg gaaacgttac cgtcaggatg gattgctggc ccatgtcatc
    12901 ggctatgtca gtgccggcgg agaaggacag gcagggctgg aaaagaatta tgatgaactt
    12961 ttgcaggggg gtgtagccac gcaggaaatc cttttgcagc aggacggccg gggacaggcc
    13021 atccccggga tcaatgtaaa agtcagaagc acagacaagg ccaccaatgc tttgggttta
    13081 accattgacg cgcgtgtcca gcaagcggtg gaaaacatta tggaccagag aattgcgcgc
    13141 ggtgcggtcg tggtcatgga ggtgcacagc aaagagatcc tggccatggc cagccgcccg
    13201 acctacaacc agtaccgggt gcaagagtat ttggctgatc tgcgcagtcc tctgctgaat
    13261 cggacgttga gcagctatta tccgggttcc gtgtttaaaa tcgtggtcag cactgccctg
    13321 gtggaagaga atctggtccg gcagggagaa acatttcgtt gcagcggcca atacgttttt
    13381 aaccaggatt tggccattcc ttgctggaaa gaggaaggac atggggaagt aagctttgaa
    13441 caggcctttg ccggctcctg caatcccacg tttattcagc tgggtctgcg tttggggaaa
    13501 aaccgcttat tgcagtatgt tgaccaattg catttaacca accccgagct aagcggtctg
    13561 cccgggcagc aaaaagacag ttatgtcagc attgaccccg gtgatccggc catggccaac
    13621 gcctgcctgg ggcagagggg cgtgatgctg acccccgtgc aaatcgcttc tttgctggcc
    13681 accatcgccg atgatggctg ctggtcgccg ccgcagctgg tcaaatatac gttggacggg
    13741 tccggacaga tgaggcagtt ggatacggtc cccggggagc aagtcatccg ggtggctacg
    13801 gcccaaaaaa tacagtccat gttggcaggg gtggtcactg atggcaccgg tcaggccgct
    13861 cgtctggacg aagtcgcagt agccggcaaa acagctacca gtcaaaccgg cagaatcaag
    13921 gaaaatggag aggaagtcct gaatacctgg tttgccgggt atctgccggc cgatgcaccg
    13981 cgctgggtga ttgtggtgct ggcagaggaa ggaaaatcag gggctgaaaa ttgtgcaccg
    14041 gtttttaaag aaattgcccg ggagttgctg gccataaacc cctgagcgtg ccgggagaga
    14101 gcttccgtgc caacgccgcc ggctgccaag atataaaccc ctgagcatgc cggcagagcg
    14161 atcaggcttc atcagaggca atcatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnata aacccctgag catgccggca gagcgatcag gcttcatcag aggcaatcat
    14341 ttcccacacc cgatgtttca caaaaaggtc cagcaaatca ccgtccaact ccccatcttt
    14401 gaccataaag cccagtattt ttaacgattg tccaatcgac agcgcttttt tataggggcg
    14461 atcggcggca gtcaaggcat caaacacgtc cgttaatgca aggattcttc cctccagcgg
    14521 aatttcttcg cctttcagcc cgagtggata gcccttgccg tccagatgct cgtgatgaat
    14581 gccggcaaaa aacggcacat ccttgagttt ggtggtaaaa gggattttat ccagcatccg
    14641 ccgcgtcacc tgcacatggt cttccataac gattctttcc tcatcggtca gggttccttt
    14701 ggtcaccgac agatggatga gttcatcctg cgtaagccag ggctgttcct ggccggctgg
    14761 atcagcatag cggcgcgcgc caatttgctg cagctcctgt tgaatcgcat catcaacgaa
    14821 agtcgccggg tcatcagcac gaatgattaa agcgcggctt tgcgcaatca cctcttgctg
    14881 ttcggcccag gcgtcttcca aagccttcag ctgtacttca ctggcttctc ctgcgcttgt
    14941 cagttccagt tttttctcca gatattcgct gtgcgttttt tgccaaatgt agtccagacg
    15001 ctgcaggatc agttccagcc ggcgttcaat gcgcgagcat ttattcatga cttcaagcgg
    15061 cgtggcaatt ttgccaatgt catgcagcca ggccgacatc aggagttgtt tggtgcgggt
    15121 ctcgtcaaaa accacctctg cccagcagcc ttctgtttct gcttcaatca cagcggccag
    15181 ggcaccgctc aggagtgcca cccggcgggt atgattcgca ttataaggcg tgcgggagtc
    15241 aatcgccgtg gccatgactt cgacaaaaga attgaacaat tgctcaatgt ccgcgattaa
    15301 ctgctggttg gtcagcgaaa tcgcagcctg cgaagccaga gaggagatga ctttctcgta
    15361 aaccggggag aaggggatgg gctgattgtt ttcgtccagc gcgttaatca actgcagtac
    15421 gccgatgacc tcgccttcat gattctccaa aggagtcacc agcatcgaga ccgtacggta
    15481 gccggtaatt tggtcatagt ttttcgggcc ggaaaaatca aatcccgccg cggcatacac
    15541 atcggggatg ttaattcgct cattgcgcag tgctgcatag gcagaaacgt tttccaggcg
    15601 catcggcacc ggcggcagat caatcggctc gcctttgccc cctttaaagg tatgcagtgt
    15661 ttggttttga atgattttaa agaccagcaa atcgccatcc cgcagataga gcgtaccccc
    15721 atctgcattg gtgatgcggc gcgcttcata aacaatcatt tccagcaggc ggtcatgatc
    15781 cttttccatt gacaaggcaa tgccaatgtt caagatttct tcgatctgat ggaggatatc
    15841 ggtgttggtt tgtttcacag ggagatcacc atgccttcac gggcggccgt cacctgggca
    15901 tactgccggc gcgcgtcctg ctcgatacgg tccatgtcat catcgtcgtg gaaattggag
    15961 tggtgaaaaa ggatgagttg cctggcacgg gccgcttcca ggagcttaat gcccgcctgc
    16021 caggtcgaat gcccccagcc aacccgggaa ggaaggccgc cgacaccgtt atactcatcg
    16081 tcggtaaaat tggcatcata aatcagtaca tctgcctggg cggcaaacgc aatcatggct
    16141 gggtccattt gcggactgtg ttccatgtcc gtcatataac agcagctgcg tccggcgtga
    16201 tccacccggt agcaaaggca gccgccggga tgattgcttt ccagggtcct gacttgaatt
    16261 ccgtcctcca gatccaaggt catgccgctg atcaattcat tcatcgtgat ggtagcgccc
    16321 atctgttcca gactgaccgg aaaatgctcg tacatcattt gtccgcgcat caaatcacca
    16381 aagttcagat tgactttggt ctggccgtaa agattgaaag cattgccttt gatgaaagcc
    16441 ggggcaaaaa aggggaaccc ctggatgtga tcccagtggg tgtgactgat gaaaatatca
    16501 ccgttaatcg gctggtttgc ctgcagcagc gcctgcccca gtttgtgaat gccggtgccg
    16561 gcatccatga taagcaggcg cgaaccaatt tggatctgga cgcaggaagt attgcctcca
    16621 tacctgactg tttgcatccc cggaaccggt atggaacctc gcactcccca gaataccact
    16681 ttgaaatccg gttgggtcat ggcctcaact tccttaacca tgaaaatccg ccgcttaatc
    16741 cttcttttga tcaagaatcc tggatatgcc ttccagattg gaatagaagt cttcctcgcc
    16801 gctgttcaat aaagtgcgca gctcggcatt catcgccgcg cccgaatcat ataccttttt
    16861 gcccaactcg cggccttttt cggtcagata gagattgacc acccggcgat cctgagcgaa
    16921 caaacgacgc tccaccagac cttccttttc cagtttgtca accatggtag tcaaagaaga
    16981 gttgtcaatt tgggcacgat tgccgatctc ggtcaacgtg cagccgtctt tttccagcaa
    17041 ggaaaaaaga ataaatgact gcgccatggt aactccgtaa ttttccagcg tcgagccaat
    17101 atgtttttca acccgtttca tggttgattt taaacggtaa cagataaagg ttgtaaagtc
    17161 ctgcattttt aactccttac tatcaaactg ttttgaacac aaatattatc tttatttaca
    17221 ttatgatgtc cgaacatcac cattttgtca aggacgttac accaggacgt tcttagtgta
    17281 acagctttaa attaatcctg ttacagaaag aacgtcccag tgtaacacgg cgtaacagaa
    17341 aaaattacct ccatcaaagt ggcacttttt ttcgaccaat ttcatatagt ataaagcgct
    17401 caaaatatat ggaggtggga ttgtgtccgt tttatactgg gcattggcta ttgcggcggt
    17461 tgtcaagggc agtctgctcc tgtttggcta tctgaacaat caggtgtttc cccagccatt
    17521 ggatgccgag gaagaaaagc gttatctgga tgaattgcaa aacggcagtg aagaagcgcg
    17581 caacaaactg atcgaacaca atctgcggct ggtggcgcat gtggtgcgca aatacgagag
    17641 cagcggcgaa gaactggaag atctgatttc catcggaatt atcggactga tcaaggcgat
    17701 taaaacctac aaccatgaac gcggggtcag actggccacc tatgcggcgc gctgcattga
    17761 aaacgaagtg ctgatgcatt tgcgcaatat caagaaactg aaacaggaaa tctccatcta
    17821 tgatcccatc ggttatgaca aggaaggcaa tgaaatctcg ctgattgata tcctgaccag
    17881 cgacagcgaa attattgaac agattgatat ccgtatgcag gaagaaaaag taaaaaacaa
    17941 tctgggggtg ctgaaccggc gcgagcgcca ggttatcgag atgcgttacg gcgtcttcag
    18001 cgggcttaag gagacccagc gggatatcgc caaaaagttg ggcatctcgc gttcttacgt
    18061 ttccaggatc gaaaaacgcg cggtcaacaa gcttatgaaa gaaatcagct gtcaaggctg
    18121 agggagaacg ccttctgttt ggagaaaaac caaagcccgg gagaagaacg gtgactgcac
    18181 tgagaccgtc gccgctgcct cccgggcgta aagcggaaat tagccgtgga tcaagcgggg
    18241 cagacagcgg gtacagaccc aaagtgcctg gcctttgtgt tttactgcca tgacataggc
    18301 atcatcctcg gaaataccgc agaaactgca ttcctggggg atataattga ttttaattcc
    18361 ttgcttgtgg gcgtggaccg cctcttccga aaaatcaggt gcttcccgtt gttccagtga
    18421 caaggcgccg gtcggacaga cgcccagaca gaaaccggct ccgtcacaaa gctcttcctt
    18481 gattaccctg gctttgccgt taatcagcgc cagggcgcct tctgcacagg gactgatgca
    18541 gacgccgcag ccgttgcaca gttcttcatc aattttgaca atggtccgca aggccatgat
    18601 gattcctcct ttcagcgcag caaattcacc ggtcagtcgg ggaatttcga ttgacggggc
    18661 ccgctgccgg cccgccggcc gtttaagact tgatcatgat caacatcatt ttaaagggtt
    18721 ggactgcctt cagagcatgc ggatgatgcg ccggcataat aatgctctca ccctgtttga
    18781 caatgtaggg atggccggag atgatgatct cggcttcccc gtccagcagg taaaccatgg
    18841 catcaaaggg cgccgtgtgt tcactcaggc cctgcccctg atcaaaggca aacaaggtga
    18901 ccgtaccggc agatttattg atcagcgttt tgctgaccac cgcatccttc tggtattcca
    18961 cactggcggc cgaatttgcc acctgtggca aaatctcctt gtcagcacct acgataattt
    19021 tttcatccat gtccgacacc tccgttaaat tgttttagtt ttagcccagg atggcggcca
    19081 gatcttgttc cggggtggca atcggtttca aatcaaagtt cttcaccagc acgtcgagca
    19141 cgttggggct gacgaacgca ggcaggctcg gccccaggcg aatatccttg atgcccaggt
    19201 acagcagggt cagcaatacc gccacggcct tttgctcata ccaggagatt accatggaca
    19261 gcggcaggtc gttgacgccg caatcaaaag cttcgctcag agcgaccgcg atttgtaccg
    19321 ccgagtaggc gtcgttgcac tgtccgacat cgagcagacg gggaatcccg ccgatggaac
    19381 ccagttgttt gtcaaagaaa cgatatttgc cgcagcccag ggtcaaaacg gccgtattgt
    19441 caggagtctg ctccacaaaa tcggtaaaat agttgcgtcc gctcttggcg ccgtcacagc
    19501 cgcccaccag gaagatgtgc ttgaggtcgc cggatttgac ggcgccaatg atctggtcgg
    19561 ccacgctcat caccgcgttg tgggcaaacc ctaccataac cgagccgaga tcttcgtcac
    19621 tgctgaaccc gggcatttcc tgggctttgg cgataaccgc ggcgaaatca ccgttttgta
    19681 cataacgcac gcccggccag ccgaccggac cggtggtaaa aatattttcc cggtacgtat
    19741 cctgcggttc ctgaatgcag ttggtggtca tcaggatggc gccggggaaa ttggcaaatt
    19801 cctttttttg attctgccag gccgttccat agttgccata cagatgagga tattttttca
    19861 gttccggata gccgtgggcc ggcagcattt ctccgtgggt atagacatag attccgctgt
    19921 cccgggtgac ttccagtaat tcttccagat cttttaaatc atggccggag accagaatgc
    19981 atttgccggc cttgtgtccc agcggcactt cagtcggcac cggatgacca aaggaaccgg
    20041 tattgcccgc atccagcaat tccatggtgc gcaggttgat tgcaccgcat tgcaggacca
    20101 gacccagcca gtcattcaat cccaaagctg gattgccgat ctgggccagt gcttcctggg
    20161 taaatgcata aacagcatcg tcttcctggt tcagaatgct ggcatgatag gcataagcag
    20221 ccacgccttt taatccataa atcaataatt cctgcagaga aaccaaatcg gcatcaccat
    20281 tgagatctac caggaagccg atttcctgtc cctggctgac cagttcggca acactgccgg
    20341 cgggtacaaa tttgacggac ggatcggtac tggccggagt gtgaccggcc gcggccattt
    20401 tttcctgcag ttgggtccgc agcgccacgc actcagacag catctctgca aagctttccg
    20461 ggtcaaagtt tacattggtg agggtgctga agagcacctt catcgtaaaa cggttaacct
    20521 ttccatcaac gatgccatgc tttcttgctt cggccgcata aatggagagt cctttggcag
    20581 catacagcag taaatcctgc agggctgcca catcgggttt ctttccgcat acaccgacta
    20641 cggtgcaggc gatgccttta ctggtttgtt cgcattgatt acagaacatt tttactcctc
    20701 ctcaaaaatt tatgaattag gataggatct atgattaact ggacataaaa tatccagtta
    20761 gagaacaaaa aatttattct tccgatggca aatcggatga tgaatcatat gccgaatcaa
    20821 aatcaccagc ggccaaatct gcgatggatt ctttgctcag ctcagccaca aaagcctgct
    20881 gcgcccgggc cagcaccgga gaaatccgac accggggctg attggggcaa ataaaaccgc
    20941 ttgccagaca aggattgatg gcgatcggtc cttcgatagc ctccaccaca tcgcgcacct
    21001 tgatgtcttg cgctggacgg gccagccgga ctccgccctg ggtgccgcgc acggtgctga
    21061 ccagaccgtt gactgccaat aaatgaatgg tctttttgag aaaatcctcg gggatatcct
    21121 gccgttcaga tataatcttg gtggacaaga gttctccgta gggatgagct gccagttcca
    21181 acatggtgcg cagcgcatat tcacattgcc gggtcagttg catgcgtaca tcctccaaag
    21241 tggactaagt taatcaacat taactgatat ttagtgtaat atgcaaatgg ctgcttggca
    21301 agtggttttt tatatttttc accgatattt ttataatgac caattttgcg gtatgatatg
    21361 acgtagaagt gcattccggg gcagcaaagt caatccagca taatcatacg gacgcttgac
    21421 atattattaa agtacacggt gaaggaggaa gaaaaaatgt ctgaactcag aagggatctg
    21481 gtcaagaaca actgggtgtc ggtcgccgag catcgtgccc tcaagcccaa cgattttccc
    21541 atcgccaaaa aggcgctgga gtcatctgca acgggcggtt tttgtccgtt ctgtgagggc
    21601 aatgaaccgt ttaccccgcc ggagatcgca gcctgccggc aggggagtga gcccaataca
    21661 aagggctggc aggtgcgggc gattcccaac aagttttctg ccttcgagct ggaaggtgaa
    21721 ctggaggaga cccgccttgg cgttttctcc cgttacaacg gcctgggcaa gcacgaagtc
    21781 cttatagaaa cccccgaaca caacgttgat tttcatcagc tgtcggttga acgcatggaa
    21841 accatcatta aaatgttgcg cgatcgctac aacgctctgg ccgttgaccc gcgcatcaaa
    21901 tatattcaga tttataaaaa ccgcggtatt ttcgccggtg cctccctgga gcacagccac
    21961 agccagatcg tggccctgcc cttcgtgcca tcggaaaatc aaggtgtcat cgactattat
    22021 caggccaacc gccgctgcct gctctgtgac attgccgacc aggagcgcca cggtgaacgg
    22081 ctggtggccg agagcgagca ttttatcctg ctatgtccgt atgccccgcg ttttccgtat
    22141 gaaacctgga tcatccccca gcagcaccgg gcgcatttcg gtgacatcaa ggacgaggag
    22201 attcatgatc tggcccaaat catcaagaag ttctgcaatg tgatgattga aagtctgtcc
    22261 gatccttcgt ataatctggt catcaatact gcgccggtgg atgtcagcga tcagcctggg
    22321 tatcattggt atatcgaagt cactccgcgt ttgctggtca acgctgcggt cgaaattgcc
    22381 agcggggtct atatcaaccc ggtcgcaccg gaactggccg cggccatgct gcgggaaaag
    22441 atgaacccag tataataaat attttgaggt aaacagatga ccaacaaact atatccacgc
    22501 atgtatgtcg atacgctttt ggatgttccg ctggaggagc tggcggcgct cggcgtgcgt
    22561 gcctttattc ttgatctcga caataccatc accgaatgga acagccagga gttgcgcgcc
    22621 gaagtggaag cgtggtttcg caccattcgt gagaggggct ttaaggcctg catcctgtcc
    22681 aacaacggcg aacagcggat tgtgcagatt gccgcgcgcc tggagattcc attcatacac
    22741 cgggcccgca agccgatgcg cggcgcgttc ctgcaggcgg tggagatcat ggggatgcag
    22801 ccggatgcgg tagcggtgat tggcgatcaa atcttcaccg atatcctggg cggcaatcgt
    22861 gccggactgt ataccattct cgtcaaaccc ctggcgcgca gggagtttta tggcaccaaa
    22921 atatcgcggg cgatggagtt ttttgttttg cgtcgttatc ggcgccgatc aaaactcgcc
    22981 aacaacggtc gtccgggttt gtaagaaact tgaaataagc accaaaacat gaaataatct
    23041 ttggcatagg caccccaccc gggaccttgt gaaaatgaga ggcagggagc aaacgtgcag
    23101 aaatcagccg acatattatt gggtttgctg ggcaatccgg tggaacacag cttgtctccg
    23161 ctgatgcaaa acagtgcgat acgttggctg ggactgcctt atgtgtatct gccgttttgt
    23221 atcgaaccgg aacacctgcc ggcggcggtc gccgcgattc gtgctttgtc catgggggga
    23281 gtgaatgtga ccattccttt caaggaagcc gtgattccct acctggatgc cttgtcagag
    23341 gatgcccggc tgtgcggggc agtcaacctg attcacaacc agggggggaa actcctcggg
    23401 ctcaataccg acggggaagg gtttatccgc agtctgcggg aagaagacct gcacccgcag
    23461 ggacagttcg ttctcatcgg ggccggcggt gcagcccggg ccatcgcggg agcgatcagc
    23521 cagtttccca ttgagcagat tgttttcctg gatatcgatc ccgcccgcgc gcatcatctg
    23581 gccggccata tcgcgcagca aaccggctgt tgctgccagg cggcggtgat gaaccgggaa
    23641 aattttgccg ccgccagtgc gcaggcggat tttatcgtga attgcagttc ggtcgggatg
    23701 tttcctcata tcgagggctg cccggttgat accttcgctc aagccaaaga cagtgcagtt
    23761 gtatatgatc tgatctataa cccattacag acccgctttt tacaattggc atcaactcgg
    23821 ggactcaaga ccgtcagtgg attgtcaatg ctggtacacc agggcgcctt gtcgctcgag
    23881 atcctgaccg gctgtcaggc cccggtgtcc cacatgaaag cagtggtgtc agattattat
    23941 gcacaacaac aaaggattta cgctggctga acttatattt tgcctggcct tgctgacgat
    24001 attgatggcg gtggccatgc cccatttcag tacggcgctg accgagctta aactcaccgc
    24061 cgatgcccgt cagatggccg gggttttgcg aatggcccgc accgaagcga tcaccaccgg
    24121 aaccaaccgg caggtcgtgt tctacgtgaa cgacagccag cgtccccgtt atcaatctgt
    24181 cggcagagcc agttacccac tcagcccggg tgtgaaattt gccggcacca aaccatttaa
    24241 ctcccgcttc aagaatcagc cggcctgcat attcatgccc aacggccgcg ccgtgcaagg
    24301 gggaacggtc acgctgaaag ccgaccaaaa atacaaatat gtaatcgtac tcaacacgac
    24361 cggacgcatc agagtctctg acacgccgcc ggaaagctgg taagatggga gatagaaaaa
    24421 gatgatgata ttgcaattgc tgtttatttt ctttgccaga atcattgatg tttccttggg
    24481 taccatccgc accattctga tcattcgcgg agaaaaatgg ccggcggcca tcattggttt
    24541 ctttgaaatc atggtctata ccgtcgccct gggaatggtc gtaggctcgc tggacgatcc
    24601 cctgcgcttg ctggtgttct gcactggttt tgccatgggg gtgctggtgg gcagtatcat
    24661 cgaagaaaaa ctggcgctcg ggtttcgcgg tctgcaggtc accatcgacc tggaaaatgc
    24721 tcatattgtc gatgaattac gggacgaagg ctacccggtc acctgttggc aggcgcaagg
    24781 caaactgggg cccaaactgg tcttgaacat tatcaccaaa cgcaatctgg cgcaaaagat
    24841 cactgatcaa atctgtgcct gtgacccgaa tgcctttgtg gtctatatgg aaccgaagtt
    24901 ttttcagggc ggctatatca aaaagaaata atgctgcagc cggagcaaaa cagatacccg
    24961 ttggagaaat tcgaaaggtg tggctatgcg aaaaaatatc gttttaattg gcttcatggg
    25021 gactggtaaa agttcgatag gggtgcggct ggcatccaaa ttgaacgcag aattcatcga
    25081 catggatcga gaaattgaaa ccctgaacgg catgtcggtc agcgaaatct ttcggcgcta
    25141 tggagaacgc cgctttcgct cggaagaaac cctgatggcc aggaaactgt ccgcgcggga
    25201 aggcctgatt atcgccaccg gcgggggcgc tgtgctgaat cctgagaata ttgccatatt
    25261 tcgtcaaaat agtattataa tattgttaga ggccagcgca gaagatatct ggacccgggt
    25321 aaaccgcaaa aaagggaccc gccctttgct caaaggagcc tccaatgttg cggacatcga
    25381 aaaactgttg gcggtcagag ctccatatta cgattgtgct gatatccgcg tcaataccag
    25441 cgacaaagag ctggagttca tcatccggga aatcttgcaa aagataaaag aagtatctga
    25501 cccaggcggc gacaattatc agaacgcatt gaaccgaccc caatagagga aatcgaaaaa
    25561 aggaagcgaa tgattaagca catgaagcag gcgagcccac agtttatttg gcaaaaaggc
    25621 ggtaactgat atgtcaaaac gcaagatgat tggtgagata ctcgtagata tgggcctgat
    25681 cacccaggag cagcaacaaa gcgtgctcga agaacagcag aagaccggtg ataaattcgg
    25741 taacattatg ttgagaaaag gtcttctcac cgaacagcag ctgttggaaa cgctggagtt
    25801 ttcgctgggc atcccgcagg tgcagctcag caagatggac atcgacccgg aagcggtcaa
    25861 acttctgccg ccgcagttga tcaggctgca tcaggtcctc cccattgccc gacgtaaaaa
    25921 cattttgacc ctggccatgg ccgatcccct gaaccagcag gccatcgatg atgttcgact
    25981 ggccaccaat atggatgtgg tgccggtgct ggccagtgag cgtgacatgg ataccgccat
    26041 ccgccaatac ctggcgtttc gtctggaccc cggcatggag aatatcgtca acgaattggg
    26101 cctggacgac aaggccagtg ccatgcggct caaagaactg caaaacatga aactggatga
    26161 agacgccccc atcatccgca tggtcaactc catcatggta caggccgtgc aggggcgctg
    26221 cagtgacgtc catattgaac cgcaggccaa ggatctgcgg gtgcgtttcc gggtggacgg
    26281 ggagctttat gaggtgctga ccctccccca gctcagtcag gcagcagtgc tgtcgcgtat
    26341 caaaatcatg gccaatatgg atatcgccga aaaaagaatc ccccaggacg gacgtttccg
    26401 catggtggtg gatggccgcg aagtggattt ccgggtttcc accctgccta ccgcactggg
    26461 agaaaaagcc gttatgcgta tcctggaccg caacaatgcc ctgacccggg tggaccagtt
    26521 aggactcagt cagcgcaata aagaatatct cctgtccttg tcgcgacggc cgcatggcat
    26581 gctcctggtt acgggcccta ccggttccgg caaaaccacc accctctatt cggtgctggg
    26641 agaagtcaac tccgtcgata aaaatatcat caccctggaa gatccgattg aatataccct
    26701 ggccggcatt aatcaggtgc agaccaatcc ccgtgccggc ttgaccttcg ccagcgggtt
    26761 gcgctccatc ctgcgccagg acccggatat catcatggtc ggtgagatca gagaccagga
    26821 aaccgcccaa ctggcagtcc aggcggcgct gaccggacat ctggttttat ccaccctgca
    26881 taccaacagt gccgccggca ccatcgcccg catagaagac atgggcatcg aggattttct
    26941 cttgtccagt tccctgagcg ggattgtttc ccagcgtctg gtccggcagt tgtgtcccaa
    27001 ttgcaaacag aaatacatat tggaggagga gaccgcctgc aaactcggga tgccggaaga
    27061 aagcgggcaa accttctatc gctcggtggg ctgcaacatg tgccgccagc tgggctatca
    27121 aggccgcatt gccctgcatg aggtaatggt ggtgggaccc aaagttcgcc agctgatcaa
    27181 caagggtgag aactccgagg atgaaatcgc tgtcgccgcc atcaaggaag gcatgatcac
    27241 cctgcgcgac gatggcttgg agaaggcccg acagggaaaa acctccctgg aggaagtcat
    27301 gaaagtcgtt ttactggaag gaaaataacg catgcaaacc aatcaaaaca tcatggatat
    27361 tgtaaaaaat ggcgtcgaat tgggtgcttc cgatatccat ttgacggttt ttcgcccgcc
    27421 tatctaccgg gtcaacggca agctgttctc gttgccggaa gacaaggtgc tggacggcga
    27481 ggataccatc cgcctggcca cggaattgtt ccccaatgaa aaaatcctgc caaccctgca
    27541 ggaaaccggg caggtcgatt tttccaattc catgcccgga gtggggagat tccgtgtcaa
    27601 tacctttata cagcggggtt cctgggcctg tgccatccgt ctgatcccga tgcatgtgcc
    27661 gcgtttggag acgttggggc tgccgccggc gatcagcgaa ctggcgcaaa aggaaaaggg
    27721 cctgattttg gtcaccggcg tcaccggcag cggcaaatca accaccctgg cggccatggt
    27781 cgattacatg aatcaacata aaaatttaaa catcctgacc ctggaagatc ccattgaata
    27841 tttgcaccgg ca
//
LOCUS       DAOTHZ010000024        27366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2002_length_27366_cov_6.309582, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000024 DAOTHZ010000000
VERSION     DAOTHZ010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 27366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27366
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2002_length_27366_cov_6.309582"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1777)
                     /locus_tag="P5549_04470"
     CDS             complement(143..1777)
                     /locus_tag="P5549_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRW12356.1"
                     /translation="MPITEILAHNAMQYGEEISIIEINPELQEKHEFNWRELELMEPN
                     PIAEFRREMTWREFDDRANQFANLLLHRGIQKGDQVAILMMNCLEWLPIYFGILKTGA
                     IAVPLNYRYTADEIKYCLDLSEADAVIFGFEFIERMETIIDIIPNVKLRFYVGDNCPT
                     FAESYDHLATACSIEAPAIVLTDEDDAAIYFSSGTTGFPKAILHNHTSLISSCRTEQN
                     HHGQNRDDNFLLIPPLYHTGAKMHWFGSLLAGSKAVLLRGVKPNWILKAVSDEQVTIV
                     WLLVPWAQDILDAIDRGEIKPEDYQLDQWRLMHIGAQPVPPSLIRRWKEHFPHHQYDT
                     NYGLSESIGPGCVHLGVENIHKVGAIGIPGYQWEVMVVDEAHQPVAQGQVGELAVKGP
                     GLMTCYYNDPEATDAVLKDGWLFTGDMARMDEDGFIYLVDRKKDVIISGGENIYPVQI
                     EDFLRSHQAIKDTAVIGIPDQRLGEIAAAIIELKPGCVVSEEEIQEFCKALPRYKRPR
                     KVIFDEVPRNPTGKIEKPRLREIYRVNNIVEVQTNN"
     gene            complement(2053..2487)
                     /locus_tag="P5549_04475"
     CDS             complement(2053..2487)
                     /locus_tag="P5549_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12357.1"
                     /translation="MDNCCQGNYQDHCHDDCHHHGHFPFPFPGLKRPYVLVASIPRNR
                     QIGVSPKTKAIKLVFAKDYTTRGLVDVDYTIDMWQGWKQIPITIRRTIDKKTGRRVWL
                     VIPLVPLKGGVTYKIRIKSVLIFCDGTKVSKCKLIVFTTGCR"
     gene            complement(2825..3646)
                     /locus_tag="P5549_04480"
     CDS             complement(2825..3646)
                     /locus_tag="P5549_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12358.1"
                     /translation="MPCCQDSQAECNNRIDYSNIIIGGERILPFVFELDLDLSVLNPP
                     AGENQRFCYRVTGVGENISTYVSLSHWVLSLCPSITLEQIANVSVTIGDEQQTVIIGE
                     NVALFLPPDTDPTTGCPGLKFDFAINKVLDDENSTGLFCFELTTPYPVGSVNVCVKGG
                     QVSSAALAICGPVCTDEDLCRVTASQLVNVCVPVTIRPYAFTGPARTICCGVPTVGDM
                     PCGGVVNGTCTFYVSQQICVEVPINFGSSTTPGETYVDCGSASAGECVCPPVNND"
     gene            complement(3689..4093)
                     /locus_tag="P5549_04485"
     CDS             complement(3689..4093)
                     /locus_tag="P5549_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12359.1"
                     /translation="MTSNYVRYSKNEQRIRSQSDCSGHVKPISDREQVSANLPGTCSR
                     LAYQRATVNVPVCVKPFTLLGSANTYCCDEPLITDLHCHPHGHKQICCFTVSQEICVE
                     IPVHFGARVCAGESWVECHESSAESCADCDTK"
     gene            complement(4167..4739)
                     /locus_tag="P5549_04490"
     CDS             complement(4167..4739)
                     /locus_tag="P5549_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRW12360.1"
                     /translation="MNPRIKNYLYFTIFITFLVLAYQLYTDLSADYKNPDSQLQDKVN
                     LPPNESTDLAAPDFTIFDQQGREVKLSDFRGQPVVLNFWASWCPPCRKEMPHFNEVYQ
                     ESREQVVFLMVDLIDGTRETESKGKAFVREQNFSFPIYFDSQLSAAQAFGVGPIPITF
                     LINAEGNVVQVYQGAIDKDALYSGIRLITK"
     gene            complement(4755..5426)
                     /locus_tag="P5549_04495"
     CDS             complement(4755..5426)
                     /locus_tag="P5549_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRW12361.1"
                     /translation="MEYLITFLEGIITFISPCLLPMLPIYISYFAVQNDSNNRYAALG
                     NAVGFVLGFTLVFTTMGAFAGTIGGFLQEYSKVVNLIAGGIVLLFGLNFMEIINIPLL
                     NATRQVETKTLNLGFFTAMLFGIVFSISWTPCVGAFLGSALMLAASSGESLKGILMLL
                     CFSLGLGIPFIASAILIDNLMSVFAFIKRNYRTINMLSGSLLVVVGIMMMTGWWGSWL
                     AALTF"
     gene            5572..7713
                     /locus_tag="P5549_04500"
     CDS             5572..7713
                     /locus_tag="P5549_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12362.1"
                     /translation="MNQKYQANNQISQPTMEELLLLLDSVYNGIVSIDEQKRVRIFNK
                     SAEQIFAMDRNEIIGKPYQDVFPDGPLLEILQFGSVEDNRKFEYKGKTLLSNRTPILD
                     KKNKIKGAVAIVQDISNLEGMNRILNDALQANEQLSTILKKSLDSLFITDAEGRVLSV
                     NEAYTKITGIKSDDILGKTLYELVEKGLYKRAAALMVIESHQPVSYTEKTKTGRTAVF
                     VGTPIFNQEGKLVNVLVNIHDITELESVSIELADTKALKDELNAVIQASFDGIIVTDG
                     NGVVLSMNDSYQRITGLQREELVGRNMHELTEAGIYDHSDVLMVIESKKAVTFTQKLK
                     NGKSILVTGNPIINDNNELFRVITNARDLTELDRLRQEVEHANHLRRHYENELNRIRM
                     EDSFIAEATSSYDLKELIARVSKVDSTILIQGESGVGKEIVARELHHQSHRHEKPYIK
                     INCAAIPESLLESELFGYEKGAFTGARKEGKMGIFELADQGTLFLDEVGDIPLHLQVK
                     LLRVLQESEVSRVGGNLPIKIDVRVIAATNRNLREMIKNKQFREDLYYRLNVVPIFVA
                     PLRERGEEIEPLILHFLEYFNKKYDLNKTIAPQLISVMQNYNWPGNIRELRNVIERAV
                     VTSPDNVIHEINFPGSEETLFSSESNDLPAIPLKTAIEEYEKELLQKYIEKYGSSRKV
                     ASILGTSQTTIWRKANQYGIQLRDSSFPDNH"
     gene            complement(7781..8539)
                     /locus_tag="P5549_04505"
     CDS             complement(7781..8539)
                     /locus_tag="P5549_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRW12363.1"
                     /translation="MSDEAVKIDVIEFMGKKLNFLDLGKEVGPDVPVYPGHMKTAFWW
                     HKTHEETKFSLGESSKYPYGFGVKGIITCDHTSTHVDAVYHFDPNKSHLSVDKITLPE
                     LITPAAWIDLSFVQGRVHITLDAVKKGLDAAQVTLKPGMSLLYWTGNEPYGTIDPFRY
                     LSNYPGLDKEATTWLLDQGLVNIGTDSPSLDTPADLDYPNHTVHAEREVIHTESLVNI
                     TKIPRHDNFYYAMFPLKFVGLTGSPIRAFAIWED"
     gene            complement(8588..10015)
                     /locus_tag="P5549_04510"
     CDS             complement(8588..10015)
                     /locus_tag="P5549_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRW12364.1"
                     /translation="MSRLTSELITTLDESRLTGNHIRVISAAVLGDMLEFFDLALISF
                     VIAFIAVPWGLTYTASASILLAAGFGSIVGAVMFGNIADRIGRRPVFMFTIIFSGVMT
                     GLMYFTPEGNWIYLVILRFFVGLALGGLYCVDLPLVQEFAPAKYRGRVSGIVTAFIPI
                     GTLLASTSAAYLTPYIGWRGLFLVGLIPVLLALIIRVWVPESPRWLIRNKKYDEAAAA
                     VDWVTEQHHDFRDSKRQNEKWIDDLPAQPHIKFSELFQYPRSVITTWGANVCQQTGYS
                     LFTMWGPALLALVIGKTPQEAAKLFIVVTLGGFIGRWFWSFMADHIGRRSTGMILGTG
                     AALMLMFALLNTGNFIGGTSVFWLCMIGIYFFADGGYALVGPYGSEVWPSNLRATGMG
                     SAYGMGGIGKLIGPVIVALFAGSSNLVSPKATTDAITPVYIFLAVLFVIQAVLFFYAR
                     ETKGKTMEDIDAELEEERSGKLKTA"
     gene            complement(10100..10864)
                     /locus_tag="P5549_04515"
     CDS             complement(10100..10864)
                     /locus_tag="P5549_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRW12365.1"
                     /translation="MSDLSVDLSGKVAVVTGAGSGIGRACAIALAQSGAAVAILDVNQ
                     DALEETKRLIRDCGSEALSSICDVSKVAEINKTYEAIAEHFGKIDIAVNVAGLNIQQR
                     GEEVTEEAWDKIMNVNAKGSFFCSQAAGRIMMKQQKGKIINMGSAMSVVGYVKRSVYC
                     ASKGAVAQFTKVLAGEWAPYNITVNTVAPTFINTPWGEPMFKDEDFKLDVERRILMHR
                     IGEVDDVVGAVLYLSSSAADFVTGSILMVDGGWTAW"
     gene            complement(11432..13147)
                     /locus_tag="P5549_04520"
     CDS             complement(11432..13147)
                     /locus_tag="P5549_04520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW12366.1"
                     /translation="MNILLNPKNYRDRNYPDARSKEIMLKTIQWFEDKGLAKQKEQYH
                     DKEFCKDFQDFVTQEGIFETLFLPKGYGSDPNQYYSTYRMYEFSEICGFYGAGYWYTY
                     HVSTLGLDPVLLGDNEEAKHKAAAKLKDNALCAFGLSEKEHGADIYSSEMKLTPKGDG
                     TYVANGSKYYIGNGNVASTVTVFGKIEGTDDYVFFVVDSAHPNYELIKNIVDCNQQFV
                     AEFALHDYPITDAEILSRGPKAWDDMLNTINICKFNIGSGATGIVTHSFYEALNHAYH
                     RNLYGKKVTDFAHVKRLFSDAWLRIMGMKLFGLRATDYMRIANENDKRYLLFNPVMKM
                     KVAIQGELVHELLWDIIAAKGFEKDMYFEQAVSELRGFPKLEGTRHVNMALIAKLVPS
                     YMFMPAEFEEIGRITDDRNDDYLFRQGTTHGYAKTKFHDYHIAYNSLAHLPNVKVYME
                     QIEAYKEYLMVSGQNLMKQMRDFDYLLAVGELFTMVAYGQLIIESAKIEGISDEVMNQ
                     MFDMFVRDFTAFAVDLYGKPLNTDEQIEKIQKMIKRPVVNQAEFEKVLNEEVFSLVDE
                     YIQNP"
     gene            13497..14249
                     /locus_tag="P5549_04525"
     CDS             13497..14249
                     /locus_tag="P5549_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12367.1"
                     /translation="MAMVEWTKDGTVAIMTLNNGENRHNPVWCEAVLSTYEEIMADPE
                     ICSLVLTSSDPKNFCLGVDIEWLGKRMAEKDWPAIEQWLHLNGKVFRTILMSSIPTVA
                     AVTGHAFGNGAMLAGACDFRFMRADRGYMCLPEIDLGIQFAPSMIEWMKRIMPYHFFL
                     KMQLTGQKVGANELEKYNVIMKACENGEKTVEEAVAFARTFNKPRANMAEMKNRTYKH
                     IIDIMENEDPKYYKYDPELAKKGITPIFMFTR"
     gene            complement(14407..15279)
                     /locus_tag="P5549_04530"
     CDS             complement(14407..15279)
                     /locus_tag="P5549_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRW12368.1"
                     /translation="MQKIKLLGISGSPRHGNTETLVKEALQSAAELVPADTELISLAG
                     LKINPCDGCNRCYGFSKGATWERLCPVYDDDVTMIYQKIREADGLIIGSPVYTGDITA
                     RLKALMERGACFCHYSASPVAGSIRNKVVGGIVVAFERRGGQESALQSIWRWATGILF
                     SHVVGAVPFPGDPPPQASALGGLADTCDSHTGYGKKGALAETTRTVPPTSGIYNLKSV
                     RNLGKSVGLGALAMNRGLMAIKEEGIDIPPLPLTSFPPNILQPDSYLEQVFSGKIQVP
                     SYQTIKGIAKERDK"
     gene            complement(15281..16681)
                     /locus_tag="P5549_04535"
     CDS             complement(15281..16681)
                     /locus_tag="P5549_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRW12369.1"
                     /translation="MSGFFGVVSKEDCVTDVYFGTDYHSHLGTSTGGMAIWNGKGFNT
                     SIHNIENTQFRARFEQELHSFKGKMGIGCISDMEPQPLTIRSHLGQYAISTVSKINNL
                     YEIADNEFTHRNIHFLETSKGTINPTELVSVIVDQENSFKDGLLKAQELIEGSCSILL
                     LTSKGIYASRDKLGRTPLVVGEKDGAYCIAFESCTFPNLGYTFLYELGPGEIILLTPE
                     GIQKISPPRKEMKICAFLWVYYGYPSSTYEGMNVEVMRYRNGAVMARKDQVELDMVAG
                     IPDSGIGHAIGYSNQSKIPYGRPFIKYTPTWARSFMPQSQEIRNLVARMKLMPIPELV
                     THKKLLFCDDSIVRGTQLRETVDLLYQCNAKEVHIRSACPPLVFGCKYLNFSTNRSEM
                     DLVARKAIADLEGKEPDYFEDYCDPSTDKYQCMVERICKRLNFSTLKYQNIHDMLDSI
                     GIDKNKICTYCWNGRE"
     gene            complement(16936..>17379)
                     /locus_tag="P5549_04540"
     CDS             complement(16936..>17379)
                     /locus_tag="P5549_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12370.1"
                     /translation="KSDKDTIAAILNVTDSRFQEDLLKQAKKAGKIPMDYQIPDEYRN
                     NTPEKLAALIKPYQEKGYFKPFPFGTDLTPIEIGLGGALKGFKALATGSPGKLAGKVI
                     TEIFKPIPESAHVYLDRMDLATPASAKEKVYRKLVVLALRNAGRL"
     assembly_gap    17380..17479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17480..18960)
                     /locus_tag="P5549_04545"
     CDS             complement(<17480..18960)
                     /locus_tag="P5549_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12371.1"
                     /translation="MKEYAGEVYADVGKIVDEIINYIGKDITFSMPLALGKPILIINE
                     LYRRAKEDPTINLRIVTALALEKPRGHSELEKRFLKPLSDRVFAGVLDFDYMLDLREK
                     KLPDNVKVYEFFSKAGSYLHDIEAQQNHLNSNYTHVVRDAIDFGVNVFGQLVSNQEID
                     GKMMYSMGCNTDISIEALEELNKLRSSGQKIVLVAEANPKLPFMYGDAVVEAESYDMI
                     LQGDFVDYEMFAPPKDPVAIPDHMIGINVSTLVPDGGTIQVGIGALGDAIVAGLIMRN
                     EHNEVYQEFINHINITGRCQDLINRWGSTGKFEKGLYGSSEMFVDAFMQMYKSKILKR
                     QVFDSIPLMKLINANKLDPQQIPPDIIEQLLDMKAIKPHLSQDDFAFLSKFGILKAGL
                     EYKEGSIYNGTQAYGTDMNEAENREQIRHLLGTHLLNGQVILGAFYIGPKSFYDQLNQ
                     MSEEERKQFGMSGVNKVNQLYGDEELRALQRKDGRFVNAGMVC"
     gene            complement(19221..19889)
                     /locus_tag="P5549_04550"
     CDS             complement(19221..19889)
                     /locus_tag="P5549_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRW12372.1"
                     /translation="MNNRSGGSDIELIRVIVNYGLGSKIIKAAKKYGISGGTVTLGKG
                     TIKNRLLNYIGITDEKKEIVHMATEKETAERVLEKLNQEFEFYKPHHGIAFTTSICGI
                     FGTQRMLCKSLQEESEADNSMYHIITVIVDRGKAEEVIAAATKAGSKGGTIINARGSG
                     IHETSKLFAMDIEPEKEVVIILSEFEKTEDIVALVRENLKIDKPGNGIIFVQNANKTY
                     GIHK"
     gene            complement(19879..21372)
                     /locus_tag="P5549_04555"
     CDS             complement(19879..21372)
                     /locus_tag="P5549_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HRW12373.1"
                     /translation="MSVITSKLVEVLYSVLPITAIVMLLNFTLTPIDTPLLIRFLLGA
                     ILIVIGLTVFLFGVDIGITPIGNMMGTAIVKSNKVWIVIVAGLFLGFFISIAEPDLHI
                     LAGQVDAVTSGLISKGILVVVVSIGIAVLFAVGLTRIVYNFPLYKTLTVLYGIILVLA
                     LFTSPEFMAISFDASGATTGALTVPFVLALAWGVSQLKMDSKSSEEDSFGLVAIVSTG
                     AIISVMIMSILAKTDTITGMLPTAQAMSTSIMAPFLQKLPLIAGEIVLALAPILITFM
                     VFQKIAFKLSKTALRRVLFGMLFTFVGLVLLLLGVNAGFMEVGSIVGFKMASLDNYWY
                     VIGAGFILGMVTILAEPAVNVLTHQIEEVTSGYVNRKVVMITLALGVALAVALSIIRI
                     LTPGIQLWHYLLPGYLISIALTHFVPKLFVGIAFDSGGVASGPMTATFILAFTQGVAE
                     AVEGANVLIDGFGVIAMVAMTPLIALQILGLVFKIRSKKGGVDDNEQ"
     gene            21779..23080
                     /locus_tag="P5549_04560"
     CDS             21779..23080
                     /locus_tag="P5549_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRW12374.1"
                     /translation="MMIPVSWEHLVTWLVLCFILLSVVSDKIRYDLTAFAGLLGLGLL
                     GFRETERLFSGFSDPALFTIAVVLVLSTGLAESGILTGLGQSIARKVHDPDRQILSVS
                     AVTVLLSSMMNNVGAVGLILPTVQRMARRAGLDPALYGLPIAYASILGGSLTLIGTSS
                     NIIVSAFRMQASGKPFQMFDFAPQGVAMVMAGLILWYILHLRHKKLPDREASLPSQTH
                     DTNDDIHDFDELFIAPLRTFRNTTIVLVFFLPALLLTSIGLIHPAVAFGLVVMVLLAT
                     DILPYDKAYASLNLPVLIFMGSMISVANVMQENGSLSLVVNQLIPFLQTLPPWLMIYF
                     ILLLTTLISNILNNAVAAVLMSPTAIILAQSGLLNISVDALLMAVAAGASLGIILPTN
                     QSTLIVMSSTSFSRKSFIKIGAVLFLVSGIIASLVIYMIWL"
     gene            23133..24500
                     /locus_tag="P5549_04565"
     CDS             23133..24500
                     /locus_tag="P5549_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRW12375.1"
                     /translation="MEFKQVYTPGLAHCSYVIGGKNECVVVDPSRSVDRYVQIAENFN
                     LPITAIIETHLHADFISGHMELAKRTGAQIYISAQAKANFAHYAVKDGEEFRIDTLLF
                     KMLDTPGHTPEASVFVVSDLERGEIPALVFSGDTLLIGDAGRPDLFPDIKNELAAKLY
                     HSLLRIKEIGDHVEVYPAHGAGSLCGKVLSSKLSSTIGTERMYNAAMKIHPEDLFIRT
                     FLAEMPEAPDHFSRCSEINRKGPALLADLPRPKPLSPGKFLKEIAAGAIVVDTRDQLA
                     FASAHIPKAYGLSIKGNFATFSGWVLPPDKPLLLVMESDDDLKNILPALYSVGLDNVI
                     GYLHNGMTTWAEEGLRTSYLKSISILELKDLWDKNEVELIDTRLKSEYDVMHIDGSIH
                     CPAPDVRHRYHEFIGDKPVAFICNTGNRSLLAASLMLNLSGSSNVINVIGGTSAWQKA
                     GCPLG"
     gene            24512..25042
                     /locus_tag="P5549_04570"
     CDS             24512..25042
                     /locus_tag="P5549_04570"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRW12376.1"
                     /translation="MGFFVFGNLLTDPWPWWLGGICIGLLVPLMYYFTNTALGVSTGY
                     GNLARLIFFKTRLKWLHSDAFKNIYNWRFFFIIGIVIGAFISAQLAGMLLVTSDMGRF
                     TQMFAWPFLYQALWFFAGGFLLGLGARTANGCTSGHSIHGLANFHLSSLIATVFFLLF
                     GFLTVHFISFLQAGGM"
     gene            25043..25456
                     /locus_tag="P5549_04575"
     CDS             25043..25456
                     /locus_tag="P5549_04575"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRW12377.1"
                     /translation="MSKNLIILIFGIFFGFVLSRVGASNYDMIFSMFTMENLTLAWVI
                     LTAIVTAAVGMQVFKLLGHKGYKGIDIKIKKKELTRYTALGGAFFGIGWGMSGACPGT
                     ALAQVGEGKFLAVFTILGLIAGTYIYALWLEKSQQ"
     gene            complement(25590..26381)
                     /locus_tag="P5549_04580"
     CDS             complement(25590..26381)
                     /locus_tag="P5549_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRW12378.1"
                     /translation="MVIGGVDVVALLMILLKAILIFFIGRLVINLILKQSNKLLSKKS
                     YDPMLEGFALSILKFLLYFVLIMAILQTFGVQTTSFIAVLGAASLAIGLALQGNLTNF
                     ASGALLVTLKPFKVNDFVEIGGEAGVIAEIGIFHTVIKTVDNKKVIIPNGSVTSGVIT
                     NYSAYSERRVDLTIGVDYNAKIDHVKATIEKAIVASDKILSDKPIFVRLAEMADSSLN
                     FSVRVWTRSENYWDVYFDLMENIKNKLDEESISIPYPHVEVVMKK"
     gene            complement(26559..27284)
                     /locus_tag="P5549_04585"
     CDS             complement(26559..27284)
                     /locus_tag="P5549_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12379.1"
                     /translation="MTYSAYHEQISDKVKVMLAITKDFKKIIRYGPISDYDMRNVVIF
                     PETDKKKNWIALMRPNDKNQEVSGSGHVGGSFGEICTAFAPAPAGPWQIGEVIMRSGH
                     GPSAFQAKIGPGIPPVKTQYGWLNIFHGVRSTMAGHPYSLAVAFHDLKDPTNPGKLRI
                     SAKPLLMPAAQDSIVRGDDYVHVPNVVFSCGALLGKNDTVAIYYGGNDTVMNVGLTNI
                     RILNEMCDHFGIDPLTGRHLYSL"
BASE COUNT         7506 a         6332 c         5854 g         7574 t
ORIGIN      
        1 ctttccactt gggcttgcat ttgtttggaa atcatgatgc acgtcctttc tgttttgttt
       61 atgactcgtt taagccgact gcgttgataa atatataata accgatattc aaggttcatg
      121 aatatcggtt attattatat gtttaattat ttgtctgtac ttcgacgata ttgtttaccc
      181 gatagatctc acgcaagcgt ggcttctcaa tctttccggt agggttgcgc ggaacctcat
      241 cgaagatcac tttgcggggc cgtttatagc gcggcaacgc tttgcaaaat tcctgaatct
      301 cctcttctga aacaacacaa cccggtttta attcaatgat tgctgcagcg atctctccca
      361 gtcgttgatc cggaatacca atcactgcgg tatctttgat ggcctgatgg ctgcgcaaaa
      421 agtcttcgat ttgcaccgga taaatgtttt cgccgccgct gatgataaca tcctttttac
      481 gatccaccag atagataaaa ccgtcttcat ccatacgggc catatcaccg gtaaacagcc
      541 aaccatcttt gagtaccgcg tcagtcgctt cagggtcatt gtagtaacag gtcatcaagc
      601 ccggtccttt gaccgccagt tcgcctacct gcccttgggc aaccggttga tgcgcttcat
      661 ccacaaccat cacttcccat tggtagccgg gaattccaat cgccccaact ttgtggatat
      721 tttccacccc caaatgtaca cagccggggc cgatggattc gctcaaacca tagttggtgt
      781 catattggtg atggggaaaa tgttccttcc agcgacgaat tagactggga ggaaccggct
      841 gggcgccgat gtgcattaac cgccactgat ccagttgata atcttccggt ttaatctctc
      901 ctcggtcaat ggcatccaga atatcctggg cccaaggaac gagaagccaa actatcgtga
      961 cctgttcatc ggaaacggct ttcaagatcc aattgggttt aaccccacgc agcaaaacag
     1021 ctttgctgcc ggccagcagg ctgccgaacc aatgcatttt cgcaccggta tgataaagcg
     1081 gcggaatcaa aaggaaatta tcatcccggt tctggccatg atggttctgt tccgtccggc
     1141 aggacgatat cagactggta tgattatgca aaatagcttt cggaaatccg gtggtcccgg
     1201 acgaaaaata gatagccgca tcatcttcat cggtcaagac gatcgccgga gcttctattg
     1261 agcaagcggt cgccagatga tcatagcttt ccgcaaaagt cggacaatta tcgccgacat
     1321 aaaagcgcag cttgacattg gggataatat cgataatggt ttccatgcgt tcaataaatt
     1381 caaatccaaa gattaccgca tcggcttcgg aaagatccag gcaatatttg atttcgtctg
     1441 cggtatatcg gtaatttaaa ggaacagcga tggcaccggt tttcaggatg ccgaagtaaa
     1501 tgggcagcca ttccagacaa ttcatcatca gaatggccac ctgatcgcct ttttgaatcc
     1561 cccggtgcaa caataaattg gcgaattgat tggcacgatc atcaaattcg cgccaggtca
     1621 tttcccgtct gaattcagcg atgggattgg gctccatcag ttccagctcg cgccagttga
     1681 attcatgctt ttcctgtaac tcgggattga tttcaatgat actgatctct tctccgtact
     1741 gcatggcatt gtgggctaga atttcggtta tgggcatctc gggtcattcc tccttaatat
     1801 actactgtac taccaacgga aataggtcgg gacggttgca tcgccttagc tgacgggctt
     1861 gtctgctgtc aaaatgatga ggcaccatta gctgggtcgg cagccttctt gccatacaaa
     1921 ttcgtcgctg gccgtatccg ttttcatcct catcaattat ctcgaaaatt gttgagataa
     1981 tcaatgcata attcacaacc gccgatcgaa aaacgatcgg cggttgcgaa gcattttgat
     2041 tgattgaatg gtttatctgc accccgtagt aaaaacaatc agtttgcatt tggaaacttt
     2101 cgtcccatcg cagaagatca gcactgattt aattcgtatt ttgtaagtta cgccgccctt
     2161 caggggaacc aggggaatca ccagccatac tcgccggccg gttttcttgt caatggtcct
     2221 tcgaatcgta atgggaattt gtttccatcc ctgccacata tcgatcgtgt aatccacatc
     2281 taccagaccc cgggtggtat agtctttggc aaaaacaagt ttgatcgctt tagttttcgg
     2341 tgaaacaccg atttgtctgt tacggggaat cgatgcaacg agaacatagg gcctttttaa
     2401 gccaggaaaa ggaaatggaa aatgaccatg atgatgacaa tcgtcatgac aatgatcctg
     2461 ataattaccc tgacaacaat tgtccatatt caaccctcct ttttaagaaa tctaatgtat
     2521 tgtattcaaa tgtgtcttga tgtgtcacaa aagtttatca agtggcgatg ttccaaaaac
     2581 taaatgaaaa gctttccacc ttatcatctg acaatcagcc taataatgaa ctattggcaa
     2641 aaggtgaatt gcatacaatg tttgcagaaa caattaatgg aaggataaat taattgttta
     2701 atcattcgtt tttttcatag gtgtgtaacg gacagacaga cgaaaaaata ccggggttgc
     2761 agtcccggta tttttatcgg ttcagattat ttaagaatgt ctgggggcat acagatcttt
     2821 tttcttaatc attgttcact ggagggcata cacattcgcc ggccgaagca gatccgcaat
     2881 ctacataagt ttcaccggga gttgttgatg agccaaagtt tatcggtact tctacacaaa
     2941 tttgctgcga tacataaaaa gtgcaagttc cattgactac accgccgcaa ggcatgtcgc
     3001 cgacagtagg taccccacag caaatggtac gagcaggacc ggtgaaagca tacggtctga
     3061 tggttaccgg aacacaaaca ttgaccaact gggaagccgt gacccggcac agatcttcgt
     3121 ccgtacaaac cggtccgcaa atagccaggg cagcagatga aacctgacct ccctttacac
     3181 aaacatttac tgatcctacg ggataagggg tggtcaattc aaaacagaac aaaccggtac
     3241 tgttttcatc atccagaacc ttattgatgg caaaatcaaa ctttagcccg ggacaaccgg
     3301 ttgtcgggtc agtgtctgga ggaagaaaga gtgcaacatt ttcgccgata ataaccgttt
     3361 gctgttcatc accgatggta acggaaacgt tggcgatttg ctccagggta atactgggac
     3421 ataaagacaa cacccagtga ctcaaactga cataagtgga aatgttctct cctaccccgg
     3481 taactcggta acaaaaacgt tggttttctc cggcaggagg gttcaaaact gacaaatcca
     3541 aatccagttc aaacacgaag ggcagtattc tttctcctcc gatgataata ttactgtagt
     3601 cgatgcggtt gttgcattcc gcctgggaat cctgacaaca aggcatgata acacctcctt
     3661 tcaataatac atcttatggt gattattgtt atttggtgtc acagtctgcg cacgattcgg
     3721 ctgagctttc gtggcattcc acccaggact cgcctgcaca gacccgggct ccaaaatgaa
     3781 caggaatttc cacacaaatc tcctgactga cggtaaaaca gcatatttgt ttgtggccat
     3841 gcggatggca gtgcagatcg gtgatgagcg gttcgtcgca gcaatacgta ttggcagatc
     3901 caagcaaggt aaatggtttc acacaaaccg gcacattgac ggttgccctt tgataagcca
     3961 atctggaaca ggtaccgggg agattcgcag acacctgttc cctgtccgat atcggcttga
     4021 catggcctga acagtcagac tgagaacgaa ttctttgttc gtttttactg tacctgacgt
     4081 agttagaggt caccttgttc acctcccctc aacttgttac attctattgc aacggaggca
     4141 aatgggtgac ggttggatta tattcgttat ttcgtaatca aacggatgcc tgaatacaag
     4201 gcgtccttgt cgatggcccc ctgatatact tgcacaacat tgccctctgc attaatcaga
     4261 aatgtaatgg gaataggccc gactccgaaa gcttgcgcag ctgaaagctg gctgtcaaaa
     4321 tagattggaa aggaaaaatt ttgttctctt acaaacgcct ttcctttgct ctcggtttct
     4381 cgggttccgt caatcagatc aaccatcagg aaaaccactt gctctctgct ttcctggtag
     4441 acttcattga aatgtggcat ttcttttctg caaggcggac accaggaagc ccaaaaattt
     4501 aaaaccaccg gttggcctct aaagtccgac agtttcacct cccgcccttg ctggtcaaat
     4561 attgtgaaat ccggagctgc caaatctgtg gactcgttcg gcggcaagtt caccttatcc
     4621 tgcagttggc tgtcagggtt cttgtaatcg gctgataaat cagtatatag ttgataagcc
     4681 agcacaagaa acgtgataaa aattgtaaaa tagagataat ttttaatccg gggattcaat
     4741 cccaacacct cctcttaaaa tgtaagggct gccagccagc tcccccacca accagtcatc
     4801 atcatgatcc ccaccaccac cagcaatgat ccggaaagca tattgatggt tcggtagttg
     4861 cgtttgataa acgcaaatac tgacatcaag ttatcgatca ggatggcact ggcaataaag
     4921 ggaataccaa gccccaggga aaaacacaac agcattagga tacctttgag gctctctccg
     4981 ctggaagctg ccaacataag cgccgaaccc aaaaaagcac ctacgcacgg agtccagcta
     5041 atggaaaata caataccgaa caacatggcc gtaaaaaacc ctaggttcaa ggttttggtt
     5101 tcgacctggc gggttgcatt cagcaacggg atattgataa tctccataaa gttcaggccg
     5161 aaaagcagaa cgatacctcc ggcaatcagg ttgacaacct tgctgtattc ctgcaaaaat
     5221 cccccgatcg taccggcaaa ggcacccatt gtcgtaaaga ccagcgtaaa tccaagaaca
     5281 aagccgaccg cattgccaag tgccgcatat ctgttgttcg aatcattttg caccgcgaaa
     5341 taagagatat aaattggcag catgggcagc aaacaggggg atataaaagt gataatgccc
     5401 tctaaaaaag taatcaaata ttccacaagc gaatcccctc tcataccatt gcatatcaac
     5461 ccggataaaa tattcagcgt ttttacatcg ggtattccct atgttatcct taatagtata
     5521 gctggattta tatgaattat attgtaatga ataaatcaaa ggaaaaaaag catgaatcaa
     5581 aaatatcaag caaacaatca aatcagtcag ccgacaatgg aagaactact gctgttgctg
     5641 gactccgtct ataacgggat cgtcagtata gatgagcaaa aaagagtccg tatattcaat
     5701 aaatctgctg agcaaatatt tgcaatggat cgtaatgaaa tcatcggcaa accctatcaa
     5761 gatgtttttc cagacggtcc attgctggaa atccttcaat tcggttcagt ggaagacaac
     5821 cgcaagttcg agtataaggg taaaaccctg ctatccaatc gtactccgat cctggacaag
     5881 aaaaataaaa tcaaaggtgc ggtggcgatt gttcaggata ttagcaacct tgaaggtatg
     5941 aacaggattt tgaacgatgc cctgcaagcc aatgaacagc ttagtacgat tctgaaaaaa
     6001 tccttggaca gtctgtttat aaccgatgct gaaggcagag tgttaagtgt caatgaagca
     6061 tatacaaaaa tcaccgggat aaagtcagac gacatccttg ggaaaacgct gtatgaattg
     6121 gtggaaaagg gtctatataa aagagctgcg gccctgatgg tcattgaatc tcatcaaccg
     6181 gtcagctata ccgaaaaaac caaaaccggc cgtaccgccg tttttgttgg tacccccatt
     6241 tttaatcagg aaggcaaatt ggtcaatgtt ctggttaaca ttcatgacat tacagaactc
     6301 gaatcagtct ccatcgaact ggcagacacc aaagctctga aagatgagct taatgccgtg
     6361 atccaggctt cttttgacgg tatcatcgtc accgatggta atggtgtggt tttgagcatg
     6421 aatgactctt accagcgaat caccggcttg cagcgggagg aattggtcgg tcggaacatg
     6481 catgaattaa ccgaggccgg gatctatgat cattccgatg tacttatggt tattgaaagc
     6541 aaaaaggccg taacctttac ccaaaaactc aaaaacggca aatcgatcct ggttaccgga
     6601 aaccccatca tcaatgataa taatgaactg ttcagagtta ttaccaatgc cagggattta
     6661 acggaattgg acagactgcg gcaagaggtc gaacatgcca atcaccttcg ccgtcattat
     6721 gaaaacgagt tgaatcggat cagaatggaa gactctttta tcgccgaagc aacctcatcc
     6781 tacgatctta aggaattaat cgcccgcgtc agcaaagttg attccaccat tctgattcag
     6841 ggagaatcgg gtgttggcaa agagatcgtc gcccgtgaac tgcatcatca aagtcaccgg
     6901 cacgaaaaac cgtatatcaa gatcaattgt gcagccatcc ccgaaagttt attggaatca
     6961 gaacttttcg gttacgaaaa aggtgccttt accggtgccc gcaaagaagg caagatggga
     7021 atctttgaac tggcggatca aggaacactt ttcttggatg aggtgggcga tattccgctg
     7081 catctgcagg tcaaactgtt gcgtgtcttg caggaaagcg aagtctcccg ggtgggcggt
     7141 aatttaccca ttaaaatcga tgtccgggtc attgctgcca ccaaccgcaa tttgcgggaa
     7201 atgatcaaaa acaagcaatt ccgggaagat ctctattacc gtttaaatgt agtccccata
     7261 tttgtagccc cattacgtga aagaggcgaa gaaattgagc cgctgatcct tcatttcctg
     7321 gagtacttca ataaaaaata cgatcttaat aaaaccattg ctccgcagtt aatcagcgtt
     7381 atgcaaaact acaattggcc tggaaatatc cgtgaattac gcaatgttat tgaaagggct
     7441 gtggtaacca gtcccgataa tgtcattcat gagattaact tccccggttc agaagaaact
     7501 ttgtttagca gcgaaagcaa cgatttacct gccatcccat taaaaactgc aatagaagaa
     7561 tatgaaaaag agctcctgca aaaatatatc gaaaaatatg gcagcagccg caaagtagct
     7621 tccatcttgg gaaccagtca gaccactatc tggcgcaagg ccaatcaata tggcattcag
     7681 ctgcgtgaca gttcttttcc tgataatcat taaaaaccac aaaaaagaga cttttcagtc
     7741 tcttttttgt tgggggtata tataaaagga aggggatttt ttaatcttcc cagatggcga
     7801 atgctctgat cggactcccg gtcaatccca cgaatttcag cgggaacatg gcataataga
     7861 aattatcgtg tctgggaatc ttggtgatat tcaccagact ctcggtatga atgacttccc
     7921 tttctgcatg tacggtatga ttgggataat caagatcagc cggcgtatcc aggctgggag
     7981 aatccgtccc aatattcacc agtccctggt cgagcagcca agtcgtcgct tccttgtcaa
     8041 ggccgggata gttgcttaaa tagcggaaag gatcaatcgt accgtaaggt tcattgccgg
     8101 tccagtacaa caggctcatg cctggtttta gggtaacttg cgctgcatcc agtccttttt
     8161 ttaccgcatc caacgtaatg tgaaccctcc cctggacaaa cgacagatca atccaggcag
     8221 ccggcgtgat caattccggc aaggtgattt tatctaccga caaatgtgat ttgttgggat
     8281 cgaaatggta aacagcgtcc acgtgggtcg aggtatgatc acaggtaata attcctttaa
     8341 caccaaatcc gtaaggatat ttactgctct cgcccaaact gaatttagtt tcttcgtgag
     8401 ttttatgcca ccaaaaagct gttttcatat gtccgggata aaccggcaca tcaggtccta
     8461 cttctttgcc taaatccaaa aaattcagct ttttacccat aaactcgatt acatcaattt
     8521 ttactgcttc atcagacatg aaatattcct ccttgcttaa aatatattga tcatcaattg
     8581 atttgcatta agctgttttg agtttcccgg atctttcttc ttcaagttcc gcatcgatat
     8641 cttccatggt tttgcctttg gtctcacgag catagaagaa tagtacagct tggataacga
     8701 acaatactgc caggaaaatg taaaccgggg taatcgcatc ggtcgtcgcc ttggggctga
     8761 caagattgga tgaaccagca aatagcgcaa cgattacggg tccgataagt ttgccgatac
     8821 cacccatacc ataggcagaa cccattccag ttgctcgcaa attggaaggc caaacttcgg
     8881 atccataagg tcctaccagc gcgtagccgc catcagcaaa gaagtaaata ccgatcatgc
     8941 agagccaaaa tacggatgtc ccaccaatga agttaccggt attcagtaaa gcaaacatta
     9001 acatcaaggc tgcgccggtt cccagaatca ttccagtgga gcggcggcca atgtggtctg
     9061 ccatgaaaga ccagaaccat ctgccgatga acccacccag tgtaacaacg ataaagagct
     9121 tggctgcttc ctggggggtc ttgccgatca ccagagccaa caaagcgggt ccccacatgg
     9181 taaataatga gtagcctgtc tgctgacata cattagcccc ccaagttgtg ataacactgc
     9241 ggggatattg gaacagttca ctaaacttga tgtgtggctg ggcaggcaga tcatcgatcc
     9301 atttttcgtt ctgccgtttg gagtcgcgga agtcatggtg ctgctcagta acccaatcta
     9361 cggcagcagc cgcctcgtca tactttttgt tgcgaatcag ccaacgggga gattcaggta
     9421 cccaaacacg aataataaga gccaggagta caggaatcaa tccgaccagg aatagacctc
     9481 tccagcctat atacggggtc aaataagctg cactggtgct ggccagcaag gtgccaatcg
     9541 gaataaaggc tgttacaatt ccacttacac ggccacgata cttcgcgggt gcaaattcct
     9601 gaaccaaggg caggtcaaca cagtacagtc cgcccaaagc caggccgacg aaaaagcgca
     9661 ggataaccag gtaaatccag tttccttccg gagtaaagta catgagtccg gtcattacac
     9721 cgctgaatat gatggtaaac ataaaaactg ggcgacgtcc aattctatca gctatattgc
     9781 caaacatgac tgctccgaca atggaaccaa agcccgcagc tagcaaaata ctggcagaag
     9841 ctgtataagt cagcccccag ggaactgcga tgaaagcaat aacaaagctg atgagtgcca
     9901 aatcgaagaa ttccagcatg tctcccagga cagcagcact gataacccta atatgattac
     9961 cggttagacg tgattcatca agagtcgtaa tcaattccga tgtaagtctt gacataattt
    10021 acctccttgc tattttaaat acaacctgtc gtttgtagtc ttggtttgaa aaatttgaaa
    10081 aatacgatgt caatattcat taccaagctg tccatcctcc gtcaaccatt aaaattgatc
    10141 cggttacaaa atctgccgcg cttgatgaca aatacaaaac tgcacctaca acgtcatcga
    10201 cttcaccaat gcgatgcata agaattcttc gttctacatc aagcttgaaa tcctcatcct
    10261 taaacatcgg ctctccccaa ggcgtattaa taaatgtcgg agcaacggtg ttcactgtga
    10321 tgttataagg ggcccactct cctgccagta cttttgtgaa ttgagccact gctcctttac
    10381 tggcacagta cactgaacgt ttcacataac caaccactga catggctgac cccatgttaa
    10441 tgattttccc cttttgctgc ttcatcatga ttcgtcccgc tgcctggctg cagaaaaagg
    10501 agcctttggc attgacattc atgattttgt cccaggcttc ttcggtaacc tcttctccac
    10561 gctgctggat gtttaacccg gcgacattaa ctgcaatatc aattttacca aaatgttctg
    10621 caattgcctc ataggtttta ttgatttctg ccaccttgct tacatcgcaa atactgctca
    10681 gcgcttctga accgcagtct ctgatcaggc gtttggtttc ttccaaagcg tcctgattga
    10741 catcaagtat ggccaccgct gcaccgctct gggcaagtgc aatggcgcag gcacgaccga
    10801 taccgcttcc cgcaccggtg acaaccgcta cttttccact aaggtctacc gataaatcag
    10861 acattatctc acctccaaac ccttcttgac accatcattg atttactgat tatccaaaat
    10921 aacttataat ttgatatcaa acccccttgt ctgaaaataa tagttgtttg gtaatcaatg
    10981 atggaattgt gaattacata acaatctata gagcaacttt tatgccacat tgaacagatg
    11041 gacttatctt ctatacaatg tcccaatatt accgaatctt ttgcagatta attgaaatgt
    11101 tagacttgat cgagcgaacg ttcaaaagac agtaaccgtc acgtatatga atcacttaat
    11161 taaacaaata atttgcaaac cctattttat tggattttaa ggtacgcgac tcatattcgt
    11221 gacagcattt caattatgag acaccagaag atgactccaa gaaatatcta taagtaagaa
    11281 taagtacact ggcacactaa aataaaaaaa aggtccttct tgcgaaagga ccttttttta
    11341 actgattgtt tcgaaccatc cccatgcagg catttcacct tgttttggga tgaattaatg
    11401 taaccgttat aactgattct taattcttgt tttagggatt ctgaatatat tcgtctacca
    11461 gactgaatac ttcttcgttt aaaacctttt cgaattctgc ctggttaaca accggtcttt
    11521 ttatcatttt ttgaatcttt tcgatctgtt cgtccgtatt gagcggtttg ccgtaaagat
    11581 caactgcgaa ggccgtgaaa tctctgacaa acatatcaaa catctggttc atgacctcat
    11641 cactgatgcc ttcgattttg gcactttcga taatcaattg tccgtaggcc accatagtga
    11701 acagttcgcc cactgccagc agataatcga aatctctcat ctgtttcatc agattctgac
    11761 cggataccat cagatactct ttataggctt cgatttgctc catgtaaacc tttacattag
    11821 gcaaatgagc caaactatta tacgcgatat gataatcatg gaatttggtc ttggcatagc
    11881 catgggtggt tccctgacgg aaaagataat catcattgcg gtcatcggtg atccgcccga
    11941 tttcttcaaa ttcagccggc ataaacatat agctgggaac cagcttggca atcagggcca
    12001 tattcacgtg gcgcgtgcct tccagtttgg ggaatcctct cagttcgctg accgcctgct
    12061 cgaaatacat gtctttctcg aatcctttgg cagcgatgat gtcccagagc agttcatgca
    12121 ccagttctcc ctgaatggct actttcattt tcatgacggg gttaaatagt aaatagcgtt
    12181 tgtcattctc gttggcgatg cgcatataat cggtagcgcg caagccaaac aatttcatgc
    12241 ccataatacg cagccaggca tcgctgaaca agcgtttgac atgagcaaag tcagttactt
    12301 ttttgccata cagatttcta tggtaggcat ggttaagtgc ttcatagaaa gaatgggtga
    12361 caatgccggt tgcaccggaa ccaatgttga atttgcagat attaatggta ttgagcatat
    12421 catcccaggc tttggggcca cgtgacagaa tctcggcgtc ggtgatggga taatcatgta
    12481 acgcaaattc ggctacaaac tgctggttgc agtcaacgat gtttttaatc aactcataat
    12541 tgggatgggc ggagtcaacc acgaaaaata cataatcatc cgttccttcg attttaccga
    12601 aaacggtgac agtactggcc acattgccgt tgccgatata gtatttgctg ccattggcca
    12661 cataggtgcc gtcgcccttg ggtgtgagct tcatctccga ggaatagatg tcggcaccgt
    12721 gttctttttc cgacaatccg aaggcgcaca gggcgttatc ttttaacttg gcagccgctt
    12781 tgtgcttggc ttcttcattg tcgcccaaca gtaccggatc aagaccaagt gtcgatacat
    12841 gataagtgta ccagtagccg gctccgtaaa acccgcatat ttccgagaat tcatacattc
    12901 tgtaggtgct gtagtactga ttggggtctg aaccataacc tttgggcagg aaaagggtct
    12961 cgaaaatacc ttcctgcgtg acgaaatcct gaaaatcttt gcagaactcc ttgtcgtggt
    13021 attgttcttt ctgtttggcg agccctttgt cctcaaacca ttgaatggtt ttgagcatga
    13081 tctctttact tctggcatcc ggataatttc tgtctctgta gtttttgggg ttcagcagaa
    13141 tgttcataaa aaactccttt caaattaatc aatagtgaga gttcataaag atttaaacac
    13201 ttaataatta tttacaacct gaatattaat cttattataa cagaaatatt atataacatt
    13261 cgtaaattta ttgttcgaaa gataattttt attggcagca tatatttata ttttgtatgc
    13321 ataaattatt ataagcaatt aaatatttta ttaacaatat ttatgatata ctaggatgca
    13381 aataaaggaa atgagccttt tttaattgtt tcatacagtg ataaggctca tcagttatac
    13441 gaatcgggga accccaaatt ttatatgcat taattaagta ttaagggagg gtttttatgg
    13501 caatggtaga gtggaccaaa gacggcacag tggcgatcat gaccttgaat aacggagaga
    13561 accggcacaa tcctgtatgg tgcgaagcag tgctttcgac ttatgaggag atcatggctg
    13621 acccggaaat ttgctcgctc gtgttaactt ccagtgatcc caagaacttt tgcctggggg
    13681 ttgatattga atggctgggc aagcgaatgg ctgagaagga ttggcctgcc attgagcaat
    13741 ggctgcacct gaatggcaaa gtttttcgga ccatattaat gtcatccatt ccgacggttg
    13801 ctgctgtgac cggccatgct ttcggcaatg gagccatgct ggcgggagcc tgcgatttca
    13861 gatttatgag agctgatcgg ggctacatgt gtttgccgga aatcgatctg ggcatacagt
    13921 ttgcgccctc catgatcgag tggatgaaac gcatcatgcc ttatcatttt ttcctcaaaa
    13981 tgcagttgac cggacaaaaa gtcggagcga atgaactgga aaaatacaac gtcattatga
    14041 aagcctgtga gaacggggaa aagacggtgg aagaagcagt cgcgtttgcc cgcacgttca
    14101 ataaacccag agccaatatg gccgaaatga aaaatagaac ctacaaacac attattgata
    14161 tcatggaaaa tgaggatccc aaatattata aatacgaccc ggaattggcc aaaaaaggta
    14221 tcaccccgat ctttatgttt acccgttaag cgttattaat taaggcggag ggcaacgttt
    14281 taaacgaagc tccccgcctt ttttgtgcca ttctttgcat taaaattgca gttggcgcaa
    14341 tttcatattt acgtaaggca atgaaaatca aaggtataga aaatttggtt ggcttggctt
    14401 ttacatttac ttatctcttt cctttgcaat gccttttatg gtctggtatg aaggaacttg
    14461 gattttaccc gaaaagacct gttcaagata agaatctggc tgaaggatgt tgggcggaaa
    14521 ggatgtcagg ggaagtggtg gtatgtcgat tccttcttct tttatcgcca ttaatccacg
    14581 attcatggcc agagctccca agccaacgct tttgcctaga ttccttaccg atttcagatt
    14641 atatatgccg ctggttggtg gaacggtccg ggtcgtttct gccaatgcac cttttttacc
    14701 ataaccggta tgggaatcgc aagtgtctgc caaacctccc aaagcggagg cttgcggtgg
    14761 cgggtcaccc ggaaacggga cagcccccac aacatgggag aacaagattc cagtcgccca
    14821 gcgccagatg ctttgcaagg cactctcctg ccctccccgg cgttcgaatg ccaccacgat
    14881 gccgcccacc actttgttgc gtatactgcc cgccacgggt gaagccgaat aatgacagaa
    14941 acaagcccct cgttccatta aggctttgag acgggcggtg atatccccgg tatacaccgg
    15001 agatccaatg atcaagccgt cggcttctct gattttttga tagatcatgg tcacatcgtc
    15061 atcataaacc gggcatagtc tttcccaggt ggcacctttg gaaaatccgt aacagcgatt
    15121 gcatccgtcg catggattga tctttagtcc tgccagtgaa atcagttccg tatcggccgg
    15181 taccaattcg gctgcggatt gcagcgcttc cttcaccagg gtttccgtat tgccatgacg
    15241 gggactgccg gagatcccaa gtaatttaat cttctgcatt ctattctctt ccgttccaac
    15301 agtaggtgca gattttattc ttgtcgattc cgatggaatc gagcatgtca tggatgttct
    15361 gatattttaa tgtggagaaa ttcagccgtt tgcagatgcg ttcgaccata cattgatact
    15421 tgtccgtgga gggatcgcaa tagtcctcaa aataatccgg ttcttttcct tccagatcag
    15481 ctatggcctt gcgggctacc aaatccattt ctgagcggtt ggtggaaaaa ttcagatatt
    15541 tgcagccaaa taccagtggc ggacaagcgg aacgtatgtg aacttccttg gcattgcact
    15601 gataaagcag atccacggtt tcgcgcagct gcgtaccgcg gacaatggaa tcgtcacaga
    15661 acaacagttt tttatgagtc accaattcgg gaatcggcat tagtttcatc cgggccacca
    15721 ggtttctgat ttcctggctc tgcggcatga aacttctggc ccaggtggga gtatatttga
    15781 taaagggacg cccgtaagga attttggatt ggttggagta cccaatggca tggccgattc
    15841 cagaatcagg tatcccggcc accatgtcca gctccacctg gtccttcctg gccatcaccg
    15901 cgccgttgcg ataacgcatt acctcaacgt tcatgccttc ataggtagag gaaggatagc
    15961 cgtaatatac ccaaagaaaa gcacaaattt tcatttcttt gcggggcgga gatatctttt
    16021 gtatgccttc cggtgtcagc agaatgatct ccccggggcc aagttcatat aaaaaagtgt
    16081 aacccagatt gggaaacgta cacgattcaa aggcaataca ataggctccg tccttctccc
    16141 ctaccaccag cggcgttctg ccaagtttgt cgcgggaggc atatatcccc ttggaagtaa
    16201 gcagcagaat ggagcaggaa ccttcaatca gttcctgcgc ctttaacaaa ccatccttaa
    16261 acgagttttc ctggtccaca atgacagaca ccaattccgt tggattaatg gtccctttgc
    16321 tggtttccag aaagtgaatg ttgcgatggg tgaattcatt gtctgcaatt tcataaagat
    16381 tattgatttt cgataccgta ctaatcgcgt attggccgag atgggatctg atcgttaatg
    16441 gctgcggttc catgtcgctg atacagccaa ttcccatttt gcctttaaaa ctatgcagtt
    16501 cttgttcgaa tctggctctg aactgggtgt tttcaatatt gtgaatagag gtgttaaagc
    16561 ctttgccgtt ccagattgcc ataccgccgg tgctggttcc cagatgggaa tgataatccg
    16621 taccgaagta aacatctgtc acacaatcct ctttagagac aaccccgaaa aatccgctca
    16681 attcatttac actcccttaa tgaatgaaat ccattttcaa ttcaaaatca taatataatc
    16741 gcattagaat gcaatattat ataaataaga atacatcata cattgacagg cgtccacggc
    16801 aattcatttc ctgcccaaga aataagccgg aaaaacaaga agcggcaggg catcactttt
    16861 acccctaacc gcttcaagat cggcactcat tgtgttataa agtggcattc gtgttacatg
    16921 caacgtctca atgatttata acctgccggc attacgcaga gccagcacca ccaatttacg
    16981 atagaccttt tctttcgctg aagcaggcgt cgccagatcc attctatcca ggtatacgtg
    17041 ggccgattca ggaatcggtt tgaagatttc tgttattacc ttgccggcca attttccggg
    17101 actaccggta gccaaggcct tgaaaccttt gagggcaccc cccagtccaa tttcaatggg
    17161 ggtgagatcg gtgccaaacg ggaacggttt aaaataacct ttctcttgat aaggtttgat
    17221 taaggcagcc agtttttccg gggtattgtt gcgatactcg tcggggatct gataatccat
    17281 cggaattttc ccggcttttt tagcttgttt cagcaggtct tcctggaatc ttgaatcagt
    17341 gacatttaaa atcgccgcaa tggtatcttt atctgacttn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnc tgcagaccat accggcattg acaaagcgtc cgtctttgcg
    17521 ctgcaaagcc cgcagttctt catcgccata caactgattg accttgttca caccggacat
    17581 gccgaattgc ttgcgttctt cttcactcat ttggttgagc tggtcgtaga atgatttggg
    17641 accgatgtag aaggcgccca ggatgacctg cccattcagc aggtgggtac ccagtaaatg
    17701 gcgaatttgt tcgcggtttt cagcttcgtt catgtcggta ccataagctt gggttccatt
    17761 ataaatactg ccttctttgt attccaaccc ggcttttaat atgccaaatt tactcaggaa
    17821 ggcaaaatca tcctggctga gatgcggttt tattgctttc atgtccagca attgttcaat
    17881 aatgtcgggc ggtatctgct gtggatccag tttattggca ttgatgagtt tcatgagcgg
    17941 gatgctgtca aatacctggc gttttaagat tttactcttg tacatctgca tgaacgcatc
    18001 aacaaacatt tctgatgaac cataaagacc tttttcgaat ttgccggtac tcccccatct
    18061 gttaatcaaa tcttgacagc gcccggttat gtttatatga ttgataaatt cctgatacac
    18121 ctcattatgt tcattccgca taatcaaacc agctacaatc gcatcgccca atgcgccgat
    18181 gccaacttga atggtcccgc cgtcaggcac cagggtgctg acattgatac caatcatatg
    18241 gtcagggatg gctaccggat ctttgggagg agcaaacatt tcataatcca caaaatctcc
    18301 ctgtaaaatc atgtcgtagc tttccgcctc aacgacagca tctccgtaca taaacggcaa
    18361 ttttggattg gcttctgcaa ccagaacaat cttttgtccg ctgcttctta gtttgttgag
    18421 ttcctccagc gcttcgatcg agatatcggt attgcatccc atcgaataca tcatttttcc
    18481 gtcaatttct tgattgctga ccaattggcc gaaaacgtta acgccaaaat caatagcatc
    18541 acgtacaaca tgggtgtaat ttgaattgag gtgattttgt tgggcttcaa tgtcgtgcag
    18601 ataagaaccg gctttgctga aaaattcata tactttgacg ttgtcaggaa gttttttttc
    18661 gcgtaaatca agcatataat caaaatcaag aacacctgcg aacacccgat ctgaaagcgg
    18721 ttttaaaaat cttttttcca gttcactatg tccacgtggt ttttccaagg ctagtgcggt
    18781 tacaattctg aggtttatgg tgggatcctc ttttgctcga cgatagagtt cgttgatgat
    18841 aagaatgggt ttcccgagtg ccaatggcat ggaaaaagta atgtctttgc caatgtaatt
    18901 gatgatctca tctacgattt ttccaacatc agcgtacact tccccagcgt attctttcat
    18961 acagccacct acctatccta aatatttcag gagaatcgtc cccttacaaa catgatacaa
    19021 tattcagaat aatttaacat tcataaattc agcctaatta aatctattta ccgacaaagt
    19081 gcttttacgc tgttgtccag attcaggggc agtcaatccc ttttatacag gttattcaga
    19141 tcccatagga attaattcaa atattttgtt taatcatccg cgcataaggg atctgaaatg
    19201 ctttttataa atgtaatcgc ttacttatga atcccatagg tcttattggc attctggaca
    19261 aagataatcc cattaccggg tttgtcaatt ttcaggtttt ctctgaccaa ggcaacgata
    19321 tcttcagttt tttcgaattc tgagaggata atgacaacct ctttttccgg ttcaatatcc
    19381 attgcaaaca atttgctcgt ttcatgtatt cctgagcctc gggcgttaat gatcgttccg
    19441 cccttggaac ctgccttggt cgctgcagca ataacctctt ccgcttttcc ccgatcaacg
    19501 ataacggtta taatatggta catgctattg tctgcctcac tttcttcctg taaactcttg
    19561 cacaacatcc gctgcgtgcc aaaaatacca caaatcgatg ttgtaaaggc aattccgtga
    19621 tgaggtttgt aaaattcaaa ttcttggttg agcttttcta aaacacgttc ggcggtttcc
    19681 ttttccgttg ccatatgcac gatctctttt ttttcatctg taataccaat ataattcaaa
    19741 aggcggtttt ttatagttcc ctttcctaaa gtaacggttc cgccagatat tccatatttt
    19801 ttcgctgctt tgataatttt actgcctaat ccatagttga caatgacacg gattaattcg
    19861 atatcagaac cccctgatct attgttcatt gtcatccact cctccttttt tcgatctgat
    19921 tttaaatacc aaacccagaa tttgcagggc aatcaagggg gtcatggcca ccatggcgat
    19981 cacgccaaaa ccgtcgatta aaacgttagc cccttctacc gcttccgcaa ctccctgggt
    20041 gaaggccaga ataaatgtag ccgtcatggg gcctgaagca actccgccag aatcgaaggc
    20101 aataccgaca aacaacttag gaacaaagtg ggtcagcgca atggatatga ggtatccagg
    20161 caagagataa tgccataatt gtattcccgg agtcaggatg cgtataatgg acaaggcaac
    20221 cgccagtgcc acacccaaag ccaaggtaat cataaccact tttcgattta catatccact
    20281 ggtaacttcc tcaatctgat gggtcaagac gtttaccgcc ggctcggcca ggatggtcac
    20341 catacccaga ataaaaccgg ctccaataac ataccaataa ttgtccaggg aagccatttt
    20401 aaagccaaca atactgccaa cctccataaa acccgcgtta acccccaaca acaatagaac
    20461 cagcccgaca aaggtaaaca acattccaaa caagactctg cgcaacgctg tttttgataa
    20521 tttaaaagcg atcttttgaa aaaccataaa cgtaatcagt atgggcgcca gagcaaggac
    20581 gatttctcct gcgatcaagg gaagtttttg caggaacgga gccatgatcg aagttgacat
    20641 tgcttgcgca gttggcagca tacctgttat tgtatctgtt tttgccagga tactcattat
    20701 cattacggat ataatggcac cggttgagac aattgccacc aacccgaaac tgtcttcttc
    20761 agaagattta ctatccattt taagttgtga aacaccccag gccaaggcca gaacaaatgg
    20821 aactgtcaat gctccggtag tcgctccgga agcatcaaag gaaatcgcca tgaattcggg
    20881 agatgtaaat agcgctagaa ccagaatgat tccatataat accgtcaacg ttttatataa
    20941 aggaaaatta tacacgatgc gggtaagacc aaccgcaaac agcactgcaa tgccaatgga
    21001 taccacgacc actagaatgc cttttgaaat caatcccgag gtaaccgcgt ctacttgtcc
    21061 ggccagaata tgcagatcag gctcggcaat tgaaataaag aaacccaaaa acaatcctgc
    21121 aacgatcacg atccaaacct tgtttgattt cacgatggcg gttcccatca tattgccaat
    21181 gggggtgatg ccgatatcaa ccccgaataa aaaaacagtc aatccaatca caatcagaat
    21241 tgcacccagg agaaacctta tgagaagggg ggtgtcaatc ggggttagtg taaaattcaa
    21301 caacattact attgctgtaa taggcaagac tgaatataaa acttcaacta gtttcgatgt
    21361 aattacactc aaacaatcct cctcctttca ttatgataag catagcattt gttcttatgc
    21421 tttttggttt tcccgcctgt tttggagata cccttattag ccgggatatt ttgaaccaaa
    21481 atatccgaaa aagggaaacc atcgatgtca gactgcgatg ttcctgggtt tacctagcgg
    21541 ggttgtcttt tcttaatatc aggtttcgct atccccggca gccaaacact gcgtttttga
    21601 aggaacaaca aagtgacgct gtttcgccag tactctcccc aatagaatac catattaacg
    21661 gtatatgccg gcaaaagaaa taacctactg cttcttttac aggagattgg tctggtataa
    21721 taaaaacagt aagcaaccct atcacaacac cattccctgt ttaaaagggg tgagcacaat
    21781 gatgattcct gtctcttggg aacacttggt tacctggttg gttctctgct ttattctctt
    21841 atcagtcgtc tctgacaaaa tccgttatga tctgacagcc ttcgccggtc tgttggggct
    21901 gggtctgctt ggctttaggg aaacagaacg gttgttttcc ggtttttctg acccggcatt
    21961 atttacgatc gcggttgttt tggtgctgag tacgggcctg gctgaatcag gaatcctcac
    22021 tggtttgggg caaagcattg cccggaaggt gcacgacccg gaccggcaaa tcttgtctgt
    22081 ttcagccgtg acagttctct tgtcgagtat gatgaataac gtgggtgcgg tggggttaat
    22141 cctgccgacg gtgcagcgaa tggcccgtcg tgccggactg gatcctgctc tctacggact
    22201 cccgattgct tatgccagca tcctgggcgg ttctctcacc ttaattggga catcttccaa
    22261 tattattgtg tcagccttta gaatgcaggc ctccggcaag cctttccaaa tgtttgattt
    22321 tgctccgcag ggggttgcca tggtcatggc cggattgata ttgtggtata tccttcatct
    22381 tcgacacaaa aagctgccgg accgggaagc atcgctcccc tcccaaaccc atgacactaa
    22441 tgacgatatt catgactttg acgagttatt tatagctccc ctgcgcacgt tcagaaacac
    22501 caccatcgtt ttggtttttt tcctgccggc cctgttgttg accagtatcg gcctgatcca
    22561 tccggcggtg gcttttggac tggtggtgat ggtattgctt gccactgaca tcctgcccta
    22621 cgacaaagcc tatgcatctc tcaacctgcc cgtattgatc ttcatgggca gtatgatcag
    22681 cgtggccaat gtgatgcaag aaaatggttc gctgtccctg gtcgtcaatc agttgattcc
    22741 ctttttgcaa accctgccac cctggttgat gatttatttt attctattgc tgacgactct
    22801 tatctccaat atattgaaca atgcagtagc ggcagtttta atgtcaccca cagccatcat
    22861 ccttgcccag tcaggattgc tgaacatcag tgtggacgcc cttttaatgg ctgtagctgc
    22921 tggagccagt ctgggaatta ttcttcccac caatcaaagc accctgatcg ttatgagcag
    22981 cacttctttc tctcggaaaa gttttataaa aattggcgca gtactgtttc ttgtatccgg
    23041 aatcattgct tcccttgtca tatatatgat atggctttaa ccctggaata gataaaaaag
    23101 cgtttaagaa tggaaaggag ttgttgtatt atatggaatt taaacaagtt tacaccccgg
    23161 gactggccca ttgttcttac gtgattggcg gtaaaaatga atgtgtggta gtcgatcctt
    23221 cccgttccgt tgaccgttat gtccaaatcg cagagaactt taatctcccc attacggcaa
    23281 tcatcgaaac ccatctgcat gcggatttta tctccgggca catggaactc gccaaaagaa
    23341 ccggcgcgca aatctatatt tcagcacaag ccaaggctaa ttttgcgcac tatgcagtga
    23401 aagatggaga agaattccgc attgacaccc ttttgttcaa aatgctcgat accccgggtc
    23461 ataccccgga agcatccgtc tttgtcgttt ccgacctgga aaggggggaa ataccggccc
    23521 tggttttcag cggggatacc cttttaatcg gagatgcggg cagaccagac ctctttcctg
    23581 atatcaaaaa cgaattggcg gccaaactct accatagtct gttaagaatc aaggaaatcg
    23641 gtgatcatgt cgaagtctac ccggctcacg gggctggctc cctgtgcggc aaagtgctct
    23701 cttcaaaact gtcgtccacc attggtacag aaagaatgta caatgctgct atgaagattc
    23761 atcctgaaga tttgtttatc cggacatttt tagctgaaat gccggaagca cctgatcatt
    23821 ttagccgttg ctccgaaatc aaccgcaagg gaccggcgtt actggcagat cttcccagac
    23881 ccaagccatt atcgcccggc aagtttttaa aggaaatcgc agcaggcgct atcgtggttg
    23941 acacccgtga ccagttggcc tttgccagcg cccatattcc caaagcctac ggtttgagta
    24001 tcaaaggaaa ctttgccacc ttttccggtt gggtactgcc tccagacaaa ccacttcttt
    24061 tggtgatgga atcagatgat gatctgaaaa atatcctgcc tgcactatac agcgttggtc
    24121 tcgacaatgt cattggctat ttgcataatg gtatgaccac ttgggctgaa gaaggattgc
    24181 ggaccagtta tctaaagagt atttccatcc tggaattaaa agatttatgg gataaaaatg
    24241 aagttgaact gatcgacact cgtctgaaga gcgaatacga tgtcatgcac attgacggct
    24301 ctattcactg cccggctcct gatgtcagac atcgatacca tgagtttatc ggcgacaagc
    24361 cggttgcttt tatttgcaac accggcaacc gttccctatt ggccgccagc ttgatgctta
    24421 atctaagcgg aagcagcaat gtcattaatg taatcggcgg cacatcagcc tggcaaaaag
    24481 caggctgtcc gctgggataa ggggtgtttt tgtgggcttt tttgtcttcg gcaatttgct
    24541 gactgatcct tggccatggt ggctgggcgg tatttgtatc ggtcttttgg ttcctttgat
    24601 gtattatttc accaatacgg cattgggggt ttccaccggt tacggaaacc tggcccggct
    24661 tatattcttt aaaacccgct tgaaatggct gcattcagat gcttttaaaa atatctacaa
    24721 ctggcgcttt ttcttcatca tagggattgt catcggtgct tttatctctg cgcaacttgc
    24781 cgggatgttg ttggttacat ctgatatggg aagatttaca cagatgtttg cctggccatt
    24841 cctctaccag gctttatggt tctttgccgg tggtttcttg ctggggctgg gggcacgcac
    24901 ggccaatggt tgcacatcag gccacagtat tcatggtctg gccaattttc acctttccag
    24961 cctgatcgcc accgtattct ttctgctttt tggttttttg acagtacatt tcatcagctt
    25021 tttacaggca ggagggatgt aagtgagcaa gaatctgata attctgatct ttggcatatt
    25081 cttcggtttt gttttgagca gggttggtgc atctaattat gatatgattt tttccatgtt
    25141 tacaatggag aaccttactt tggcctgggt gattcttacg gctatcgtta cggcagccgt
    25201 tgggatgcag gtgtttaagt tgctgggcca taaagggtat aaaggaattg acattaaaat
    25261 taaaaaaaag gaactcaccc gctacacagc actgggcgga gcgttcttcg ggattggctg
    25321 ggggatgtcc ggagcctgcc cgggaactgc cctggcccag gttggagaag gaaaatttct
    25381 ggccgtcttt actatcttgg ggttgattgc gggcacttat atttacgctt tatggcttga
    25441 aaaaagtcag caataatcgc aagccaagta aatatttcac caattcttcc atagcaaaac
    25501 cgtcaactcg ccaatgaaat gcaaaactcc caacagattc gagcgatgtt gggagtttgt
    25561 cttgtcttgc aagtgtagat gttacccggt tatttcttca tgaccacttc gacatgggga
    25621 tacggaatgg aaatactttc ttcatcaagt ttattcttga tattttccat caggtcaaag
    25681 tacacatccc aataattttc cgaacgagtc cacacccgga cagaaaagtt gagcgagctg
    25741 tctgccattt ctgccaatct cacaaatatc ggtttgtcag agagaatctt gtcactagcc
    25801 acaatggctt tttcaatggt tgctttgaca tgatcaattt tagcgttgta atccaccccg
    25861 atagtaagat ccactcttct ttccgaatag gcactgtaat tggtgataac accgctggtt
    25921 acagaaccgt tggggatgat gactttttta ttgtcaacgg ttttgattac ggtatgaaag
    25981 atgccaattt ccgcaataac gcccgcttct cccccaattt ccacgaaatc attgactttg
    26041 aaaggcttga gagttaccaa caaggcaccg ctggcaaaat tggtaaggtt cccctgaagg
    26101 gccaagccga tcgccagtga agctgctccc aaaaccgcaa taaaagaagt tgtttgcact
    26161 ccgaaggttt gcagtatagc cataatcaat acgaaataca acaggaattt caaaattgac
    26221 aatgcaaacc cttccagcat cgggtcgtat gatttcttgg acagaagctt gttggattgc
    26281 ttcaatatta aattgataac cagtcgacca ataaaaaaga tcaggattgc tttcagcaga
    26341 atcattaaca gggctacaac atctactcca cctattacca ttcatttcac ctcctgtatg
    26401 cattcttcga cgttaaatga ctatatcctg caatatttca cattattaaa attttaaatg
    26461 caaataccat atcgttatca ttaaacacaa aaaatggatt ccgtttaatg tggactgcaa
    26521 atcaataaaa attccctgtt tgacatatcg aagtaccgct atagagaata caaatgtctt
    26581 cctgtcaatg ggtcgatgcc aaaatgatca cacatttcat tgagaatccg tatattggtc
    26641 agaccgacat tcatgacagt atcattaccg ccataataaa tagctaccgt atcattcttg
    26701 ccaagcaagg cgccacagct aaacactaca ttggggacat ggacataatc atcccctctc
    26761 acgatactat cttgggcagc gggcattaac aacggtttgg cactgatacg aagtttacca
    26821 ggattggttg gatctttgag gtcatggaat gccactgcca gcgaataagg atggccggcc
    26881 atggttgaac gtaccccgtg aaagatatta agccatccat attgggtctt gacaggtgga
    26941 atgcctgggc ctatcttggc ctggaatgca cttggaccgt ggccgctgcg cataattact
    27001 tccccgattt gccaaggccc ggcaggagct ggagcaaaag cagtacatat ctctccaaat
    27061 gagccgccga catggccaga acctgacact tcctggtttt tgtcattggg acgcatcaag
    27121 gcaatccagt ttttcttttt atctgtttca gggaatatga caacattacg catatcgtaa
    27181 tcagatatag gaccatagcg aataatcttc ttaaagtcct ttgttatagc cagcatcact
    27241 ttgactttat cggaaatttg ctcgtggtag gcactgtaag tcaagacata caaatcactc
    27301 ctttaattga caattgaaag gtcattcgag gataagcttt taattgtgta ttctcactcc
    27361 ttgcct
//
LOCUS       DAOTHZ010000025        26547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2106_length_26547_cov_7.117167, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000025 DAOTHZ010000000
VERSION     DAOTHZ010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 26547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26547
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_26547_cov_7.117167"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="P5549_04590"
     CDS             complement(<1..847)
                     /locus_tag="P5549_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRW12380.1"
                     /translation="MDSKTMHMDFDHNLFVSQLQHRKADIEACLSKKLPGEDVFPPLI
                     HKAMHYAVFNGGKRLRPIMVMESGKIAGYVGEALENLACALEMIHSYSLVHDDLPAMD
                     DDDLRRGKPTCHIVFGEANAILAGDGLLTEAFQLLARTAALPGVEPGRLLQVIAEVSA
                     AAGSRGMIGGQVMDLNEQYQQGQDSLAQLHQLKTGALFTASLRIGAILGGLDAAGLTA
                     LTQYAGHFGLAFQITDDILDVEGDPSLLGKPVGSDEKNLKLTYPACCGLEEARNMATR
                     EAEACL"
     gene            complement(1002..1223)
                     /gene="xseB"
                     /locus_tag="P5549_04595"
     CDS             complement(1002..1223)
                     /gene="xseB"
                     /locus_tag="P5549_04595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRW12381.1"
                     /translation="MKKQSFDQALQRLEEIVEQLESGQLGLEESLARFEEGVRISMFC
                     QEELQKTDGKVSLLMKKMNGEMELLDFEA"
     gene            complement(1204..2427)
                     /gene="xseA"
                     /locus_tag="P5549_04600"
     CDS             complement(1204..2427)
                     /gene="xseA"
                     /locus_tag="P5549_04600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRW12382.1"
                     /translation="MKEFITVSALNEYIGALLEADPILQGIWVKGEISGFRLFQQSGH
                     MYFTLKDGDAAVSCVMFRSRNQGLKFLPEDGMEVLLFGAVGIFSKQGRYQLYVENMQP
                     FGAGALYLQLEKLKKELDQQGYFAMERKKPLPAMPHCVGVVTSQDGAALRDILKVIRQ
                     RCPSVRVVVAHSSVQGVDAPRELAAGIGSLNAYGQVDVIIVGRGGGSLEDLMAFNSRE
                     VVEAISLSVIPVISAVGHEVDVTLADLAADVRAATPTQAAQMAVPDQTVLRDQLHSIN
                     RQLTRAMQKNLRRQAEALDYLMMRRIWRDPGEFLEKRHRDLRQASQALIKAMQKNLQT
                     RQHGLAMAVAGLDQLSPLKVMGRGYAILSHQQTIIKSIEQVSPGMQLKARLSDGQLVV
                     EVKEKEKIAHEKTEF"
     gene            complement(2694..3098)
                     /gene="nusB"
                     /locus_tag="P5549_04605"
     CDS             complement(2694..3098)
                     /gene="nusB"
                     /locus_tag="P5549_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRW12383.1"
                     /translation="MSRRKARELAFKVMFQVDQVNAEPRTAFEYLLTEQRLADSEKEF
                     AWSLIERCLQNQATIDHLISQYANDWSIDRMSSVDRNLMRIASSEILFLEGSQAAVAI
                     DEAIEISKKYGEDNSASFINAILDKIHHHQET"
     gene            complement(3157..3375)
                     /locus_tag="P5549_04610"
     CDS             complement(3157..3375)
                     /locus_tag="P5549_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HRW12384.1"
                     /translation="MEKVFYYLMSEHRGKVIGVLLGLLASILFISYGFWRTLFIVFCI
                     LAGYFIGKKIDENTNLEVWIKNLFKQNP"
     gene            complement(3392..3916)
                     /gene="amaP"
                     /locus_tag="P5549_04615"
     CDS             complement(3392..3916)
                     /gene="amaP"
                     /locus_tag="P5549_04615"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HRW12385.1"
                     /translation="MPIIWRVLLSIYNLLLLVVAGIFVAASVSAFDPLPYLQTALNEQ
                     NRLLTGLVALLLLLLALVLLIQTWKTVEKQDDGIIVQEGLLGQICMTVPAIELLIMKS
                     VRQIEGIKDIRPEIKKDKTGLTVKLHMMINPALNVPEVTAATQKIVKEQLEQVGGLQV
                     NEVKVRVDDFKAGK"
     gene            complement(3985..4377)
                     /locus_tag="P5549_04620"
     CDS             complement(3985..4377)
                     /locus_tag="P5549_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRW12386.1"
                     /translation="MKRPEIANEMGVVRIADEVVSTVAGLAAIEVEGVASMSGGWGTD
                     LVEKLGKKNFGKGIRVEVNDQVTTIDIFLSIVYGYAIPQVAENVQKEVRSAVEMMTGL
                     QVAAVNVHIVSVSMAKPAVEEVAGDEAE"
     gene            complement(4500..5843)
                     /gene="accC"
                     /locus_tag="P5549_04625"
     CDS             complement(4500..5843)
                     /gene="accC"
                     /locus_tag="P5549_04625"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRW12387.1"
                     /translation="MFAKILIANRGEIAVRIIRACRDLGIATVAVYSQADRNSMHTAL
                     ADETVCIGGAPAGQSYLNISNIIAAAQNTGAEAIHPGYGFLAENAYFAELCETNGIKF
                     IGPQPQVIKLMGDKVKAREVVSREGVPLVPGSEGAVLNRAQAVAIAEQIGYPVMIKAS
                     AGGGGKGMRLAHNRSTLEEALNMARMEARSAFGNDEVYIEKYIEEPRHIEFQILGDEF
                     GQTIHLGERDCSLQRRNQKLLEEAPSCMLDHELREKMGAMAVKAAQAVDYTSAGTIEF
                     LVDRDKNFYFIEMNTRIQVEHPVTEMVTGIDIVKAQIRIAAGEKLDISQSDVCINGWA
                     IECRINAEDPDNNFLPSPGQITEYWCPGGPGVRVDSSAYSGYIITPYYDSMIAKLIVW
                     GRTRTEAIERMRRALLEFRVGNVHTTIPFHLRVLGNAFFQKGEVYTNFIQRRMGG"
     gene            complement(5854..7731)
                     /gene="accB"
                     /locus_tag="P5549_04630"
     CDS             complement(5854..7731)
                     /gene="accB"
                     /locus_tag="P5549_04630"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRW12388.1"
                     /translation="MKKIAITDTSLRDGHQSLWATRMTTDDMLPILAKLDDIGYHSLE
                     VWGGATFDVCMRYLNEDPWERLRIIRRNIKKTKLQMLLRGQSIVGYTHYPDDIAERFI
                     DQAAENGIDIFRAFDALNDIRNLETSLKAIKKTGKHAQACVVYSVSPVHTMEHFVETA
                     LQLKDMGADSICIKDMAGLLQPYVSYELVKALKEAVQLPVQLHTHYIGGLAVVSCIKA
                     AEAGVDVIDTASVPMAFGSSQPPVETIVRALQGGAYETGLDLHALFEIADHFEDLRKH
                     KGFERGVTRISDMKVFEHQVPGGMISNLVAQLEEQKASDRLPEVLAEIPRVRKDLGYP
                     PLVTPTSQIVGVQAVLNVISGERYKLCPGEVKDYVRGLYGKPPAPIDAEVQKKIIGEE
                     EVVTVRPADLLAPGWEKARQEMADISDNEEDILSYALFPQVALKYFVNRKEQQDEDIP
                     QILSADSLGQEAAQKEEMIQKTLPQVRKKPVSKGDDEMNIDELRELILLFDQTSIAEL
                     ELQKDSLKINLRKTAGSKAITPEPIAAAAREPAYDAAKLVEILSPMVGTFYAAPSPDA
                     PSFARQGDHVKKGQTLCIVEAMKLMNEIKAECDGRIVEIAVENAEPIEYNQLLFLIEK
                     D"
     gene            complement(7950..8486)
                     /locus_tag="P5549_04635"
     CDS             complement(7950..8486)
                     /locus_tag="P5549_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HRW12389.1"
                     /translation="MIRLNQIKRWLVIAAFVLIPLLVILLLNIVIEHRQGQPVVQDTA
                     GIEQLQMPADEVVGGVNEGLGFFPEYRIERERVRGKQMELLRSIAANPNQEQKVRDNA
                     ALKLVQLADNLAREMQTETLLKAKGCQECAVILEGQRLTIILDQKVVGDEARDELLAL
                     SSAVSGVKKENIVITCRR"
     gene            complement(8492..9055)
                     /locus_tag="P5549_04640"
     CDS             complement(8492..9055)
                     /locus_tag="P5549_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12390.1"
                     /translation="MLEEWIRGGSSDNGENRYKKWSRYILVIIICLGMLALVWPTGQE
                     PPVEVQEKRTAASVESAQQKQVRELENILSQVEGAGQVRVSLSLASDGLKSYAANTKN
                     DVRETVEQDRNGGERQIREENQSSEITVSSGSALLVEEKAPQITGVLVVADGADRPLV
                     KEALGNAVAVLLHLSPHQVTVLPRREE"
     gene            complement(9062..9643)
                     /locus_tag="P5549_04645"
     CDS             complement(9062..9643)
                     /locus_tag="P5549_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HRW12391.1"
                     /translation="MTTLYEIVRNLLVIIMIASFLELILPAGRLRPLVRFSVGLFVLI
                     AILSPTLEFLFADQRFALGFWDYRVDSGMERSIQEKGEELHHQVYQTQQEEFTAKLQG
                     QINALGSLVPGVGEVQSEVHTAADGSVSKVKVSIAAGHSPPVEQVSGIRVFSPQQEAG
                     ESEEKEVIAAKMMELISSLYEIPRERIEIDFGR"
     gene            complement(9656..10834)
                     /gene="spoIIIAE"
                     /locus_tag="P5549_04650"
     CDS             complement(9656..10834)
                     /gene="spoIIIAE"
                     /locus_tag="P5549_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HRW12392.1"
                     /translation="MKRAGLLLLLMLLILGLPVPVTASVPAASPENQALLEDALDSLD
                     LNELESYKIQVDNEIAAYTDHKSAKEWLLDFIRGGWEFNPRHLLEGVIRYLADELLAN
                     SGLLAKLIILSVLAALLLNLQQSFASDVGRVAYLACFLAMGTIAMGTFKLVLGMGQHT
                     IDNMSTFMLGMLPQMMVLVAGMGNINGSVMMFPLLMTAAAAFAAAIKNVVFPLIIMSA
                     ALHLVNGLSETIKVERLAKFVGQLAQILLGFFLTFFVGVVTLRALYASALDKVTLRTT
                     RFVTDNAIPVVGKMVGDTVEVAAGYVVMLKQALGIWGVLVILGIIVYPLLEIAVLALV
                     YKIVGAVVEPMGDARTASILEIMSTHLFLMMAATAAVALMFFIMIAIVVGMYSGAAVR
                     "
     gene            complement(10861..11250)
                     /gene="spoIIIAD"
                     /locus_tag="P5549_04655"
     CDS             complement(10861..11250)
                     /gene="spoIIIAD"
                     /locus_tag="P5549_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HRW12393.1"
                     /translation="MEITQIVGLALMVTVFLLILRQEKPVMAVLLSIAFSIFIFLMMM
                     DKMGTIINVMKELSRRAGINYFFMTTILKILGVAYLGEFAAVLCQDAGEQAVAKKVEF
                     AAKIMIAVLALPIMAAILESLLELMPG"
     gene            complement(11274..11468)
                     /gene="spoIIIAC"
                     /locus_tag="P5549_04660"
     CDS             complement(11274..11468)
                     /gene="spoIIIAC"
                     /locus_tag="P5549_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HRW12394.1"
                     /translation="MNVDILFRIAGIGILISVLCIVLKQAQKQEQAEMLTLAGVVVVL
                     IIVVQLMNQLFTVVRTVFNI"
     gene            complement(11503..12027)
                     /locus_tag="P5549_04665"
     CDS             complement(11503..12027)
                     /locus_tag="P5549_04665"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12395.1"
                     /translation="MFWLKSMGIALVIAGCGGGGLIGARRIEKRVEQLQAMRQAMGYL
                     EKEITYLQTPLPLALEHTARMAASPLPCLFAGCAHTLSRRQGISAEEAWQEALIRLKA
                     QADLQPDDLQILATAASQLGSSGIEQQKQYFVLLQEQLKIQEEKARGQVASERKLWSY
                     GGFILGTTVVLFLL"
     gene            complement(12028..12981)
                     /gene="spoIIIAA"
                     /locus_tag="P5549_04670"
     CDS             complement(12028..12981)
                     /gene="spoIIIAA"
                     /locus_tag="P5549_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HRW12396.1"
                     /translation="MGFDNQVRILDAIIDYLAPQIGRLLKTIPPPHLDGLEEIRLRVG
                     QPLLLNMGQQEYALSRQGALVKELRQAYRVQADDLYKTILSISDNSLYAFEEEIRRGY
                     ITIPGGHRVGLAGQVVMENEQIKTVKDFSSLSFRIARQMKGCSQPLLEHIYRRQPCPL
                     STLIISAPRCGKTTLLRDLARNIADGSPAGPACTVSVIDERSELAGSYRGKAQMDVGC
                     RTDVLDGCPKALGMMMAIRALGPQVIVTDEIGRQEDMEALLECSNAGVAVIASLHARD
                     LEEARRRPLIRQLLETGVFQTMITLSRRRGPGTLEEIVRWD"
     gene            complement(13153..13563)
                     /locus_tag="P5549_04675"
     CDS             complement(13153..13563)
                     /locus_tag="P5549_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRW12397.1"
                     /translation="MLRDMSEKVSYLQGLTEGLNITEGSPQGKIISGMLNVMNEMADE
                     LHLIQRDFAEMKEYLESIDDDLFELEESVLEEEEFTQIKCKNCGEKLLMEMDILDDED
                     HIEVICPSCNEVVYINDGSFDYTLDEADDDGCVN"
     gene            complement(13637..14194)
                     /gene="efp"
                     /locus_tag="P5549_04680"
     CDS             complement(13637..14194)
                     /gene="efp"
                     /locus_tag="P5549_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRW12398.1"
                     /translation="MISVNDFKTGLTIEFEDVIYQVVDFQHVKPGKGAAFVRAKLKNV
                     KTGGSVEKTFRGGEKVARAHLDKREMQYLYSDGDGYVFMDNENYEQLTISKEEIGDGS
                     KWLMENMTIGVLMHNSTIMGIELPNFVELTVVETEPGIKGDTATGATKNAKLESGAVV
                     QVPLFVNEGDRLRIDTRTGQYMERA"
     gene            complement(14207..15277)
                     /locus_tag="P5549_04685"
     CDS             complement(14207..15277)
                     /locus_tag="P5549_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRW12399.1"
                     /translation="MKRIKQRVEKLRRELQANRMEALLVTCPENIRYLSGFTGGRDAR
                     LLITLDKKYIITDSRYYTQVSLECPDWELVKATPGAADVLIALSEGFASLGFESSDLT
                     YDRFLQMQAGLKPELLPVQGLVEKLRLIKEETELELLRTAARIGDQVFTTVCQQIKAG
                     MQEAEIANLIVTGLKNGGCSREAFETIAVAGVNAALPHGQPGDYRLQPGDMLTMDFGG
                     LYQGYAGDMTRTVAIHHADTKLRDAYRQLLEAQELGVGLVQAGMAGRELDQKVRAHLQ
                     KYQLDGYFVHSTGHGLGLAVHEAPRLSASSQDILQENMAVTVEPGIYIPGWGGIRIED
                     TVIVKKGGCEVITHSDKGLLII"
     gene            complement(15356..15787)
                     /gene="aroQ"
                     /locus_tag="P5549_04690"
     CDS             complement(15356..15787)
                     /gene="aroQ"
                     /locus_tag="P5549_04690"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRW12400.1"
                     /translation="MRVLVINGPNLNLLGNRENNLYGDMSLADINYALKKLAEEKQVE
                     IDFFQSNHEGELIDQIHQAWGSVDAMIVNAGALTHYSIALMDAFKAVGIPFIEVHLTN
                     ICAREEFRHHSLLSPLARGVIFGLGAMGYSLALLAICEWFK"
     gene            complement(15994..17361)
                     /locus_tag="P5549_04695"
     CDS             complement(15994..17361)
                     /locus_tag="P5549_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRW12401.1"
                     /translation="MLQKERPGVTEESLTAAGAMALEAAREKAVAAEVFLLYNKSLSI
                     ELRDERVETLKEAEEIGVGVRVLNQGRMGFAYSSDLSPTAVLAVVEQAVNISNCMPAD
                     PDQRLVEPYSVYAQMDCFDAQIPDAALEEKIALARDCEQAARAYDSRIHLVEKSVYED
                     SQSFTLIMNSLGLQASYQDNLAGLYIALAAQEEQESQSGFAAMARRRLADLQPQECGR
                     EAANRAVRSLQAKSMASQTLPCIMEPYVATRFMSLLLPSLNGDAVLKGKSLWANRMGE
                     QVASSRLTLIDDGTLPDGLASAPFDGEGVPTRRNELIRDGALRSFLFDHYYAGKAGVQ
                     STGSGRRGSFRSLPAISSSNLMIEPGTTSPEQMIAELERGLLITEVMGMHTANPISGD
                     FSLGAAGILIENGKLSRPVRGITIAGNLHQLLQNIDALGNDQRFYGTKAAPSIRFNQI
                     SIGGD"
     gene            complement(17439..18866)
                     /locus_tag="P5549_04700"
     CDS             complement(17439..18866)
                     /locus_tag="P5549_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRW12402.1"
                     /translation="MLDKKQLARVLDTALRQGGDFAEIFVEEKQLSHVLCEDNRIERI
                     NSGREKGAGIRVLKDGRTAYVYTNELSPRGLERAAEVAARVFRAGQQENAAINLTPQK
                     SGASLAYLRMPDEVAFDEKIARVLAANQAAREVDAEIRQVTVALADVQRKLQIANSEG
                     VLAEEEQVRVRMMINAIAARDGMIQTGYESAGGAIGWELMEQVALDDMAREAAGRALT
                     MLSAAPAPLGRMPVVMHAEAGGTMVHEACGHGLEADLVQKGLSIYQGKQGQRVANEHL
                     SVIDDATLKGKYGSFGFDDEGTPGQRTVMIEQGILKQFINDRLTASNDGSPLTGNGRR
                     ESYQHKPIPRMTNTFIAPGQDDPEEIIAGTEYGLLVRKMGGGQVNTLNGDFVFEVQEG
                     YLIEQGEIKHPVRGATLTGNGPQVLLDIDRIGRDLGYSLGVCGKDGQGVPVADAQPTI
                     RIKTLTVGGIGNNENPKTNKRIRRM"
     gene            complement(18906..19835)
                     /locus_tag="P5549_04705"
     CDS             complement(18906..19835)
                     /locus_tag="P5549_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW12403.1"
                     /translation="MSFEAIVALLAATVVAGTAILYAASGELLAERSGIMNLGVEGMM
                     LVGAVLGFIFTVRTGNHWYGMAAAMAGGGGMAAIHAFLTITLRSNQVVCGLALTIFGT
                     GLSGFLGKSYQGTPISDPFRAIHIPGLADIPVLGRIFFQQDALVYLSIVIAVLMWLVL
                     YHSRWGLSIRSVGQNPAAADAVGISVSRVRYFCTIVGGILAGMGGAYLSLAYAPSWQE
                     NMTSGRGWIAVALVIFALWNPMRAILGSYLFGGVEALTFRMQTAGVNISTFYLAMMPY
                     LLTILVLVIVTIRMRSEAGAPAALTLPYHREER"
     gene            complement(19835..20902)
                     /locus_tag="P5549_04710"
     CDS             complement(19835..20902)
                     /locus_tag="P5549_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW12404.1"
                     /translation="MIQYKFIKRETESLASHFIVPVLSIVLALLFGWIFLWLYGINPA
                     ETYLRMWQGAFGSSYAISETLVKAIPLMLLGLGLSMAFRMKLWNIGAEGQLYMGAFAA
                     SGIALFYGDLPSLVLLPVMGLAAFVAGGIWGLVPGAMRAYWDVNETITTLMLNYVAIS
                     FVESFIFGPWKDPASMGFPLSPRFSPQAYLPSIGDSRVHIGLLVALLVAVLLYFLLNY
                     TKWGYEIKVIGESHASARYTGINVTRNILLVMLISGGIAGLAGMIEVSGITHRLQQGI
                     SPGYGFTAIIVAWLARLNPFAIIMVAFLFGALLVGGFGLQIFGLPFSTVQMLEGAVLF
                     FLLGGEIFTKYHLQRSGKEGQ"
     gene            complement(20899..22404)
                     /locus_tag="P5549_04715"
     CDS             complement(20899..22404)
                     /locus_tag="P5549_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12405.1"
                     /translation="MIEMNGITKVFPGVVANDAISFQVRAGEIHALLGENGAGKSTLM
                     SVLAGLYHPDAGSIKIKGKPVRFRSPRSALAVGVGMIYQHFRLIDNFTVAENIILGAS
                     GGDIVLRSKAIEEEAARLSEKYGLEINPQAKVSQLSLGEQQRVEILKMLYRGCDILIM
                     DEPTTVLTPQEVKELFKMMRKMADQGKSIVLITHKLNEVLEVADSVTVLRRGKVVGNA
                     RTADLDEKVLTQMMVGKELTPSRREVLERSRELILQLDHLMVLGDQGNVAVKDLNLSL
                     YSGEIIGIAGVAGNGQRELVEAIAGLRSLAGGSIKMDGQEISALSVRERLARGINIVP
                     EDRLGMGLVPNLSVMENAILHNYSKPEYSRGFLLNYKKIRAYTHKLVKDFDVALADIN
                     HPINYLSGGNLQKLLLGREISQHPRLLVASYPVRGLDVAATESVFHLLLQERAKGTSI
                     LLILEDLEDIFRLADRIAVMYAGTIKAVFDIQQADLETVGLLMLGGGETQL"
     gene            complement(22582..23691)
                     /locus_tag="P5549_04720"
     CDS             complement(22582..23691)
                     /locus_tag="P5549_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRW12406.1"
                     /translation="MKFKKLGSFILLFVFLSVFVVGCGTSKDTGKAPEGEKEKFKVGF
                     VYIGTPGDAGWTYTHDEGRKYLAKELPEVETLFLENVAEGADAERSIDQLAQQGCKVI
                     CTTSFGFGDATIEVAKKYPDVVFMHASGINTAENVGTYFGKIEQMRYLTGIVAGKMTK
                     SNTLGYVAAYDIPEVVRGINAFTLGAQSVNPNVKVKVVWTHNWIDANLAKQAADSLIE
                     DGADVITQHVDATSPQIAAEEAGVFAIGYHSDMKTYAPNYSLTASVWNWGPYYVDLVK
                     SVMNNTWKSGAYWGGAEDGIIDLAPISDKVPADVKELVVAKQADIKSGKLDIFAGPLK
                     AQDGTIKVQEGQKMTDGEILGFNWFVAGVDGKIGQ"
     gene            complement(23840..24856)
                     /gene="pilM"
                     /locus_tag="P5549_04725"
     CDS             complement(23840..24856)
                     /gene="pilM"
                     /locus_tag="P5549_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRW12407.1"
                     /translation="MIQLFGGSQGIGLDIGSKKIKLARVKNTKQGIQVVWFESMDTPP
                     GMVESGNIVDPVGLGEELGKLVSKLKIKHHKVVSAVAGQQVYTRTLVMPRMKLDELKE
                     AISFQAINFLPIPVEEAAIDIFPLRDFEDEEGKKTEVFFVAVRRQQVENLDMVCNIAG
                     LNLVAVEIEPLAMNRVLRKLYPEDVNAYLNIGGTRSFFSVFKNDVLVFYRSLAFGISA
                     FYAALEMSFPNGAVRWEDIQFGQGSQYDYLTRDLIDDVTRSVEYYNLQKNKTEERIEK
                     IWLCGAGARFKGLDASLASGSGLEVEIADPMPELVLPANLSEEETLNIKYDFLIALGL
                     AART"
     gene            complement(24998..25519)
                     /locus_tag="P5549_04730"
     CDS             complement(24998..25519)
                     /locus_tag="P5549_04730"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HRW12408.1"
                     /translation="MPCQKKSTAREQGSMLLMSLLIFFMVSILMMSIMQLSALEMYMS
                     QYYYRSQQAQQLVDACLEQRCAEISQCLRSDYTITPDLPPLPLGWREDWMEMPVGDVQ
                     GRCQTSFVKLATGDGYCCYTIKFTGCFENATKTVVADLTFHYANNCDNDQAFLSRTYS
                     DNGVITDYKIIYN"
     gene            complement(25570..26049)
                     /locus_tag="P5549_04735"
     CDS             complement(25570..26049)
                     /locus_tag="P5549_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12409.1"
                     /translation="MQHRFDDNGLIMAEIILALGLATLVIAALMTVYFSAYRVFACQT
                     AFTDIQYAERAAMQMMIEDVMAAEEVECLDDSKTMRLTMDGGYISYYLQDRTVYRQGN
                     AKMPVANDISSLLFQPDSRPGYTRISMEACQGLKTHRLVCAAVPRSLVGAAPAESDK"
     gene            complement(26033..26473)
                     /locus_tag="P5549_04740"
     CDS             complement(26033..26473)
                     /locus_tag="P5549_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRW12410.1"
                     /translation="MNGWHREKGTTLVEVMVTLFLVALISSSLLGGFMISQRMIKYAE
                     RQTRASSFAYQLLEDLRAKSCPQWQEIITAVEQGTHHYTVDGTAEAGQEMQATVCLEE
                     NGDWPGLYDARVVVTWQEAGENRSLEMITLFYPALRGDRDAAQV"
BASE COUNT         6184 a         7801 c         6111 g         6451 t
ORIGIN      
        1 gcagacacgc ctctgcttct cgggttgcca tgtttctggc ttcttccaga ccgcaacagg
       61 ccgggtaggt cagcttcaga ttcttttcat cacttcccac cggcttgccc agcaacgacg
      121 gatcaccttc cacatccaaa atgtcatcgg tgatctgaaa ggccagacca aaatggccgg
      181 cgtattgcgt cagcgctgtc aagccggctg cgtccaggcc ccccaggatt gcgccgatgc
      241 gcagggaagc agtaaacaag gcgccggttt ttaattgatg cagttgggcc agactgtcct
      301 ggccttgctg atattgttcg tttaaatcca tcacctgccc gccgatcatg cctcgactgc
      361 cggcagcggc tgacacctcg gcgatgacct gcaacagtct gcctggttcg acccccggca
      421 gcgcagcggt ccgggccagc agctgaaaag cttcagtcag caggccatct ccggccagaa
      481 tggcgttggc ctctccgaat acaatatggc aggtgggttt gccgcggcgc aaatcatcat
      541 catccatggc cggcagatca tcatggacca gcgagtagga atgaatcatt tccagcgcac
      601 aggccagatt ttccagcgct tcaccgacat aaccggcaat tttgccactt tccatgacca
      661 taatgggccg cagccgcttg ccgccgttaa acacagcata gtgcatcgcc ttgtgaatca
      721 acggcggaaa gacgtcttcc ccgggcagct ttttagacag gcaagcttca atatccgctt
      781 ttctatgctg taattgggag acgaacaaat tgtggtcaaa atccatatgc attgttttag
      841 aatccattgg ttttcctttc tgtctgtcgc gccagggttt gggatcattt ggtacgttaa
      901 tagataaacg gctgtcgagc tattgtgctc ctctgttgcg cgtactcagg attcgctgtg
      961 cgcatgttgg gcgtgcttgc agcaacctct tttttatgga atcaagcttc aaaatccagc
     1021 agttccatct cgccgttcat ttttttcatc agcaggctga ctttgccgtc ggtcttctgc
     1081 agttcttcct ggcaaaacat cgaaattctg actccttctt caaatcgggc cagcgattct
     1141 tccaggccca attgacctga ttccagctgt tccacgattt cttccagacg ttgcaaggcc
     1201 tgatcaaaac tctgtttttt catgcgctat cttctccttc tccttgacct ctaccaccag
     1261 ttggccatcg ctgagccggg ccttcagttg cattcccgga ctgacctgct cgatgctctt
     1321 gatgatggtt tgctgatggc tgaggatggc atagccacga cccatgactt tgagcgggct
     1381 aagctggtca agcccggcaa ccgccatggc caaaccgtgc tgtctggtct gcagattctt
     1441 ctgcatggcc ttgatcaagg cctgactggc ctgacgcaaa tcccggtgac gcttttccag
     1501 gaattctcca ggatcgcgcc agatgcggcg catcatcaaa taatccaggg cttctgcctg
     1561 acgacggagg tttttttgca tggccctggt cagttgccgg ttgatagaat gcagctgatc
     1621 gcgcagcaca gtctgatcag gaacagccat ctgggctgcc tgagtgggcg tcgccgcccg
     1681 cacatcagcg gccagatcgg ccagggttac atccacctca tgacctaccg cggagatcac
     1741 cggaataacc gataatgaaa tcgcctccac cacttcccgg ctgttaaagg ccatcaaatc
     1801 ttccaaggat cctccgcccc gtcccacgat gatcacatcg acctggccgt aagcgtttaa
     1861 gctgccgatg ccggccgcca gttcgcgggg cgcatccact ccttgtacgg agctgtgggc
     1921 aaccaccacc cggactgaag gacaacgttg tctgatcacc ttgaggatgt cgcgcagggc
     1981 tgccccatcc tgggaggtta ccacgcccac gcaatgcggc atggccggca gcggtttttt
     2041 tctctccatt gcaaaatagc cctgttgatc cagttccttt ttcagtttct ccagttgcag
     2101 atacagcgca cccgcgccaa agggctgcat attttcaaca tacagctggt atcggccctg
     2161 cttggaaaat atccccaccg ctccaaaaag cagaacttcc atcccgtctt cgggcagaaa
     2221 cttgagtccc tggttgcggc tgcggaacat gacacaactc accgcagcgt cgccgtcttt
     2281 gagggtaaaa tacatatgcc cggattgttg aaagagacga aatccggaga tctcaccttt
     2341 gacccagatc ccctgcagga tcggatcggc ttccaacaaa gccccgatat attcatttaa
     2401 ggcactgacg gtgataaatt ccttcatgtc caacctcttc ctcccggctg ataaaaagct
     2461 gccgcacccc acccgcttgc gtaatcagag atggctggcg ggtcccggga acctttttaa
     2521 agagatcctt cgcttacgcg caggatgata aataatattt tcagcgttgt ttgtgttcga
     2581 ccatcatgag ggttcacaat aaagcaaatg ccaggctttc ctggcagctt gtaaacaaat
     2641 attgcagctc acaaggccgc cttcatcacg aaaagggacc cggcggttgt tcatcaggtt
     2701 tcctgatgat gatggatctt gtccagaatg gcgttaataa aagacgctga attatcttcg
     2761 ccgtattttt tagagatttc aatcgcttcg tcaatggcaa cggcagcctg actgccttcc
     2821 agaaacaaaa tctcgctgct ggcgatccgc atcagattac gatcgaccga agacatccgg
     2881 tcaatcgacc aatcattggc atactggctg atcaaatgat caatggtggc ttgattctgc
     2941 agacagcgct caatcaagga ccaggcgaat tctttttcac tgtcggccag acgctgttcg
     3001 gtcagtagat attcaaaagc agtacggggt tctgcattga cctgatcaac ctgaaacatt
     3061 actttaaaag ccaattcccg tgcttttctt ctgctcaacg ttttcttcct tcctgcgatt
     3121 tagtgcttct taatcgatat aagaggttac tttttatcac ggattttgtt tgaacaaatt
     3181 tttgatccag acttccagat tggtattctc atcaattttc ttgccaataa agtagccggc
     3241 cagaatacag aaaacaataa acagggtccg ccagaagcca taactgataa atagaatact
     3301 ggccagcaag cccagcagga caccgattac tttgccgcga tgctcactca tcaggtaata
     3361 gaatactttt tccatcaagg gtcaaccctc cttatttgcc agccttgaaa tcatctaccc
     3421 gtaccttgac ctcattcacc tgcaatccgc cgacttgctc cagctgttct ttgacgattt
     3481 tctgcgttgc tgcagtgacc tccggtacgt tcagcgcagg attaatcatc atatgcagtt
     3541 tcactgtcag tccggtcttg tcctttttga tttccggccg aatgtccttg atcccttcga
     3601 tttggcgtac tgacttcatg atcagcagct caatggcagg cacggtcatg cagatttgtc
     3661 ccagcaatcc ttcctgcaca ataattccgt catcctgctt ctccacggtt ttccaggttt
     3721 ggatcaaaag caccagagcc agcagcagca acagcaaagc aaccagtccg gtaagcagac
     3781 gattttgttc attcagggcg gtttgcagat acggcagagg atcaaaggcg gaaaccgatg
     3841 cggcgacaaa aataccggcc accaccaaca gcaacagatt gtaaatggaa agaagcaccc
     3901 gccaaatgat tggcataatg aaattccccc ttcatgtaag ataaccccct gcagacacag
     3961 ggggttattt agacagctta cgccttactc ggcttcgtca ccagccactt cttccacggc
     4021 aggtttggcc atgcttacag atacgatgtg tacattgacg gcggctactt gcaagccggt
     4081 catcatctcc accgcgcttc tgacttcctt ttgaacattc tcggcaacct ggggaatagc
     4141 gtacccatat acgatactta aaaatatatc aatcgtagta acttggtcgt tgacctccac
     4201 ccggatacct ttgccgaaat tctttttgcc gagtttttca accagatcgg taccccagcc
     4261 tccgctcatg gaagctactc cttcgacttc gattgctgcc aaaccggcaa ccgtggatac
     4321 aacttcatca gcaattcgaa ctacacccat ttcattggct atttctggtc tcttcatttc
     4381 caagtcaatc acctccaaca gtattatgat gttattctac cattactaac cacgaattag
     4441 caatgaaggt gaccatgtac ctcctgcaaa ttccggcagt aaagcctata caaagggatt
     4501 tatccaccca tccggcgctg aataaaattc gtatatactt ctcccttttg gaaaaaagca
     4561 ttgcccagca cccgcagatg gaaaggaatg gtggtatgca cattgcccac ccggaattcc
     4621 agcaaggccc ggcgcatgcg ttcgatggcc tcggtccggg ttctgcccca gacaatcagt
     4681 ttggcgatca tagaatcata gtaaggggtg atgatataac cgctgtaagc gctggaatcc
     4741 accctcaccc cgggaccgcc cggacaccag tattcggtaa tctgccccgg ggagggcagg
     4801 aaattgttgt cgggatcttc agcgttgatg cgacattcaa tcgcccatcc gttgatgcag
     4861 acatcgcttt gtgagatgtc cagcttttct ccggcggcaa tccgaatctg tgccttgacg
     4921 atatcaatgc cggtcaccat ttccgtcacc ggatgttcca cctgaatgcg ggtgttcatc
     4981 tcaataaaat agaagttttt atcgcggtcg accaggaatt cgatggtgcc ggcactggta
     5041 taatccaccg cctgagcagc cttgactgcc atcgcgccca ttttttcgcg cagctcatga
     5101 tccagcatgc aggaaggggc ttcctccagc agcttctgat tgcgtctttg cagcgagcaa
     5161 tcgcgttccc ccagatggat tgtttgaccg aattcgtcgc ctaaaatttg aaattcaata
     5221 tgacggggct cttcaatata cttttcgata tacacttcgt cattgccaaa ggcactcctg
     5281 gcctccattc tggccatgtt gagcgcttcc tccaaagtgg accggttatg ggccaggcgc
     5341 attcccttgc cccctccgcc ggcagatgct ttaatcatga ccggatagcc gatttgttca
     5401 gcaatggcga ccgcctgcgc ccggttcagc acagctccct cactgcccgg caccagcggt
     5461 acgccttccc ggctgactac ttcgcgcgcc ttgactttgt cccccatcag cttgatcacc
     5521 tggggttggg gcccaataaa cttgatcccg ttggtttcac acagttcagc aaaataggcg
     5581 ttttccgcca aaaaaccgta gccgggatgg atcgcctccg cgccggtgtt ctgcgccgca
     5641 gcaatgatat tggaaatatt gaggtaactc tgaccggccg gcgcaccgcc gatacaaacc
     5701 gtctcgtcgg ccagggccgt atgcatgctg ttgcggtccg cctgcgaata tacagccacg
     5761 gtggcaatac ccagatccct gcaggcacga atgatccgga cggcaatttc accgcgattg
     5821 gcaattagta ttttggcaaa cacctcatca cctctagtct ttctcaatta aaaacagaag
     5881 ttggttgtat tcaatcggtt ctgcattttc caccgcaatt tccacgatcc tgccgtcaca
     5941 ttcagcttta atctcattca tcagcttcat ggcctccaca atacacaggg tctgtccttt
     6001 tttaacatga tccccttgcc gggcaaacga tggagcatcc ggtgacggtg cggcatagaa
     6061 ggtccccacc atcggagaaa ggatctcaac cagtttggcg gcatcatagg ccggctctct
     6121 tgctgcagcc gcaatcggct caggtgtaat ggctttgctg cccgcggtct tgcgcagatt
     6181 tattttcaaa ctgtcttttt gcaattccaa ctcagcaata ctggtttggt caaacaacaa
     6241 aatcaattct ctcagttcat cgatgttcat ctcatcatct cccttgctga ctggtttctt
     6301 gcgcacttgc ggaagggttt tttgaatcat ctcttccttt tgggcggctt cctggcccag
     6361 gctgtcggct gacagaatct ggggaatgtc ctcgtcttgt tgctctttgc gatttacaaa
     6421 gtattttaat gccacttgcg ggaacagggc ataggataaa atatcttctt cattatcaga
     6481 gatatcagcc atttcctgcc gggctttttc ccaaccgggc gccagcaaat cggccggacg
     6541 aacagtaaca acctcttcct cgccgatgat ttttttctgt acctcggcat caatcggggc
     6601 gggaggtttg ccatacaggc cgcgcacata atccttgact tcccccgggc aaagcttgta
     6661 acgctctccc gaaatcacat tcaacaccgc ctgcaccccg acaatctgac tggtcggcgt
     6721 gaccagcggc ggataaccca aatcttttcg cacccgggga atctccgcca atacctccgg
     6781 caagcggtcg gaggcttttt gctcttccaa ctgtgccacc aggttggaga tcatgccgcc
     6841 cggcacctga tgttcaaata ctttcatatc ggaaatgcgt gtcactccgc gttcaaatcc
     6901 cttgtgcttc ctgagatctt caaaatgatc ggcaatttca aacagggcgt gcaaatccaa
     6961 gccggtttca tacgcgcctc cctgcaaggc acgtacaatc gtttccaccg gcggctggga
     7021 ggatccaaaa gccatcggca cgctggcggt gtcgatcaca tccaccccgg cttcagcggc
     7081 cttgatacag gaaacgacgg ccagaccacc gatatagtgg gtatgcagct gcaccggcaa
     7141 ttgtaccgct tctttcaggg ctttgaccag ttcataggaa acatagggct gcagcaagcc
     7201 cgccatatct ttaatacaaa tggaatcggc gcccatgtct ttcaattgca gggcggtctc
     7261 cacaaagtgc tccatggtat gcaccggact gacggaatac acgacacagg cctgggcatg
     7321 cttgccggtt tttttgatcg ctttcaagct ggtttccaga ttgcgaatgt cgtttaacgc
     7381 gtcaaacgcc ctgaaaatat caatcccgtt ctcagccgcc tgatcgatga agcgctcggc
     7441 gatatcatcg ggataatggg tatagccgac aatcgactgc ccgcgcagca gcatttgcag
     7501 tttggttttt tttatgttcc ggcggatgat gcgcagtctt tcccagggat cttcattcaa
     7561 gtagcgcata cagacatcaa aggtcgctcc gccccaaact tccagggaat gatagccaat
     7621 atcatcaagt ttcgccagga tcggcaacat atcatcggtc gtcatgcggg tcgcccacag
     7681 actctggtga ccgtcgcgca gactggtatc ggttatggca atttttttca ttttgcaccc
     7741 ccattacaaa gatcggccca tagcatgatc tgtgggccgc ctcagcctct gcagctgaaa
     7801 agctgtatat ataaatatac tcaggccaat gacctgagta cgatattctc atccagcaac
     7861 tctattatat aaaattaata cgtgtttttt caatatttaa ggccaagtat acaaatcatt
     7921 gcaacgactg tcgtcgatgc gaggagttgt caacggcggc aggtaatgac gatgttctct
     7981 tttttaactc cgctgacggc actgctcaac gccagtaatt cgtcccgcgc ttcatccccg
     8041 acaacctttt gatcaagaat gatggtcaat ctctgcccct ccagaataac tgcacattcc
     8101 tgacagcctt tggctttgag cagggtttcg gtctgcattt cccgggccag gttgtcagcc
     8161 agctgcacca gcttgagggc tgcgttgtcg cgaacttttt gctcctggtt ggggttggcg
     8221 gcaatgctgc gcagcaattc catctgtttt ccccggactc gctcccgttc gatgcggtat
     8281 tcgggaaaaa agcccagtcc ctcgttcaca ccgcccacca cttcatctgc cggcatctgt
     8341 agttgttcaa tcccggctgt atcctggacc actggttgac cttgccgatg ctcaatcact
     8401 atgtttaaca atagaatcac cagcaaagga atcaatacaa aggcagcaat taccaaccaa
     8461 cgtttgatct gattcaatcg aatcaccaat gctattcctc ccttcgcggc agcaccgtga
     8521 cctgatgcgg agaaagatgc agcagcaccg ccactgcatt gcccagcgct tccttgacca
     8581 gaggccggtc agcaccgtcc gccaccacca atactccggt tatctggggt gccttttcct
     8641 ccaccagcag ggcagaaccg ctgctgacgg taatctcact gctttgattt tcttcccgga
     8701 tttgccgttc accgccattg cgatcctgtt caacggtttc ccgaacatcg tttttagtat
     8761 tggcggcata acttttcaaa ccgtcggagg ccagactgag gctgacccgt acctgcccgg
     8821 cgccctccac ttgggacaag atgttttcca attcccgaac ctgtttttgc tgggcgctct
     8881 ccacgctggc cgccgtacgc ttttcctgca cctcaaccgg aggctcctgg ccggttggcc
     8941 aaaccagcgc cagcatgccc aggcaaataa tgatgaccag tatgtatcgg ctccattttt
     9001 tatagcggtt ttcaccgttg tcagacgacc cgccccgtat ccattcttcc agcaccaaat
     9061 ctcacctccc aaaatcgatt tcaattcttt cccggggtat ttcataaaga ctgctgatca
     9121 actccatcat tttagcggca atcacctctt tttcctcgct ctcacccgct tcctgctgcg
     9181 gcgagaacac ccttatgccg ctcacttgtt ctacgggggg tgaatgcccc gcagcaatgc
     9241 ttaccttcac tttgctgaca ctgccatcgg ctgcggtatg gacttcgctt tgcacctcac
     9301 ccacgcccgg caccagactg cccagcgcat tgatctgtcc ctgcagtttg gcggtgaatt
     9361 cctcctgctg ggtctggtaa acctgatgat ggagttcctc gcccttttcc tgaatcgatc
     9421 tttccattcc cgaatcaaca cgataatccc aaaaacccag ggcaaaacgc tggtctgcaa
     9481 agagaaattc cagcgtggga ctcaggatcg caatcagcac aaaaagaccg acggaaaacc
     9541 gcaccaaagg cctgagccgg ccggccggaa gaatcaattc cagaaaactg gcgatcatga
     9601 tgatgaccag caagttcctg acaatctcat acaacgttgt catcaatctt ccccttcagc
     9661 gtacggctgc tccgctgtac atccccacca caatggcaat cataatgaaa aacatcagtg
     9721 caacggcagc ggtagccgcc atcatgagaa acaaatgggt actcatgatt tccaggatcg
     9781 aagcggttcg cgcatccccc atcggttcaa ccaccgcacc gacaatttta taaaccagag
     9841 ccaaaacggc gatttccaac aggggataga cgatgatccc cagaatcacc agaacgcccc
     9901 agatgcccaa ggcctgcttg agcatgacca catagcccgc cgccacttca acggtgtcac
     9961 ccaccatttt gccgaccacc gggatggcgt tgtcggtcac aaaacgggtg gtgcgcaggg
    10021 ttactttatc cagcgcggaa gcatacaaag cccgcagtgt taccacccct acaaaaaagg
    10081 tcagaaaaaa ccccagcagg atttgggcca gttgtcccac aaatttcgcc agtcgctcca
    10141 ctttgatggt ttccgacagg ccattgacca aatggagggc cgccgacata ataatcagcg
    10201 gaaataccac atttttaatc gctgccgcaa aagctgcagc agctgtcatc agcagcggaa
    10261 acatcataac cgaaccgttg atgttgccca ttccggccac caataccatc atctgcggca
    10321 gcatgcccag cataaatgta ctcatgttgt cgatggtatg ctggcccatt cccaatacca
    10381 gtttaaacgt ccccatcgcg atcgtgccca tcgccaaaaa gcaagccaga taagcaacac
    10441 gaccgacatc cgaggcaaat gactgctgca gattgagcag cagcgctgcc agtaccgata
    10501 aaataatcaa tttggccaaa aggcctgagt tggccagcaa ctcatccgcc agataacgga
    10561 tgactccttc cagcagatgg cggggattga actcccaccc gccgcggata aaatcgagca
    10621 gccattcctt ggcactcttg tgatcggtat aggcagcgat ctcattgtcc acctgaattt
    10681 tatagctctc cagttcattc aggtccagac tgtcaagggc atcctccagc agcgcctggt
    10741 tttccggaga agctgccggc acagaagccg ttaccgggac cggcagtccc agtatgagca
    10801 gcatcagcaa caacagcagg ccggctcttt tcacatccca gcacctcgtt tcggctttgc
    10861 ttatccgggc atcagttcca acaatgattc aaggatcgct gccataattg gcaaagccag
    10921 aacggcgatc ataatcttgg cggcaaactc gaccttttta gccaccgcct gctccccggc
    10981 atcctggcaa agcacggcgg cgaattcacc taaataggcc actcccagga ttttgagaat
    11041 agtcgtcatg aaaaaatagt tgattcccgc gcgccggctc agttctttca tcacattaat
    11101 gatggtcccc atcttatcca tcatcatgag gaagatgaag atactgaaag caatgctcaa
    11161 cagcaccgcc ataaccggtt tctcctgccg caggatgagc agaaataccg ttaccatcag
    11221 ggccaacccc acgatttgtg ttatctccaa atgatccgcc cctttgaccg cattcagata
    11281 ttaaatacgg ttcgtaccac cgtaaaaagc tgattcatca attgcaccac gatgatcagc
    11341 accacgacta ccccggccag cgtcagcatc tccgcctgtt cctgcttttg ggcttgtttt
    11401 aagacaatac acagcacgga aatcaagatc ccgatgccgg cgatgcgaaa caatatgtca
    11461 acattcaaga acatccctcc atctctatac attgccgctt gcctacagca aaaacagcac
    11521 caccgtggtc cccagaataa atcctccata gctccacagt ttgcgctctg acgcaacctg
    11581 gccgcgcgcc ttttcttcct gtatttttaa ttgctcctgc aacaaaacaa aatactgctt
    11641 ttgctgttcg atgcctgaag atcccagctg gctggcagcc gtagccagga tctgcaaatc
    11701 gtcaggctgg agatccgcct gtgctttgag cctgatcaaa gcctcctgcc aggcctcctc
    11761 tgcgctgatc ccctgtcgcc gggacaaagt atgggcacac ccggcaaaca ggcagggcag
    11821 tggtgacgct gccatccggg cagtatgctc aagggccaac ggcagcggcg tctgcaaata
    11881 ggtgatttcc ttttccagat accccatggc ctgccgcatg gcttggagct gttcaactct
    11941 cttttcgatc cgtcgggcgc cgattaagcc tcccccgccg cagccagcaa tcaccagcgc
    12001 aattcccatg cttttcagcc agaacattta atcccacctc acgatttctt ccagggtacc
    12061 cggaccccgt cggcgcgaaa gcgtgatcat ggtctgaaac accccggttt ccaacaattg
    12121 tctgatcagc ggacgccgcc gggcctcctc caaatcccgg gcatgcaatg aggcgatcac
    12181 tgccaccccg gcattgctgc attccaacag cgcttccatg tcttcctggc ggccgatttc
    12241 atcagtgaca atgacctgcg gaccaagggc ccggattgcc atcatcatgc ccaaggcctt
    12301 tgggcatcca tccagcacat ccgtcctgca gcccacatcc atctgcgcct tgcctcgata
    12361 actaccggcc agttcggaac gttcgtcgat cacactgacg gtacaggcag gccccgccgg
    12421 gctgccgtcg gcaatatttc tggccaaatc ccgcaacaag gtggttttcc cgcagcgagg
    12481 cgcagagata atcaaggtgc tgagaggaca tggctgtcgt cggtaaatat gctccagcaa
    12541 aggctgcgag cagcccttca tctgtctggc aatccggaaa gacaaactgc tgaaatcctt
    12601 gacggtttta atctgctcat tctccataac gacctgcccg gccagtccca cccgatgtcc
    12661 gccgggaatg gtaatataac cccgacggat ttcttcttcg aaagcataca gggaattgtc
    12721 actgatggat agaatcgtct tatataaatc atcggcctgg acccggtaag cctgtcgcaa
    12781 ctccttcacc aacgccccct gccggctgag ggcatattcc tgttgtccca tgttcaacag
    12841 caaaggctgc cccacccgca gacgaatttc ttccaggcca tccagatgcg gtggcgggat
    12901 ggtcttgagc agccgtccaa tctgcggggc caggtagtct atgatggcat cgaggatccg
    12961 gacttgattg tcaaatccca tcacgttacc ccctttgcag ttaataaata ttggtttgcc
    13021 caggaaatta tgccaactac ttttgtcctt tttatagaca accctgttcc agaaacaaca
    13081 agagggtgcc ggtggttgac cggcacccga aaaatggttt ggcaaaatgg gttatgggtg
    13141 atacaaatta attcaattca cacagccatc atcatcggct tcgtcgaggg tataatcaaa
    13201 ggacccgtcg ttgatatata cgacttcatt acagctggga caaatgacct cgatgtggtc
    13261 ctcatcatcc agaatatcca tctccatcag cagtttttcc ccgcagtttt tgcatttgat
    13321 ctgggtgaac tcttcttcct ccaaaacgct ctcttccaat tcgaacagat catcatcaat
    13381 gctctcaaga tattccttca tttccgcgaa atcacgctgg atgagatgaa gttcatctgc
    13441 catttcattc attacattga gcataccgga aataattttg ccctgggggc tgccttcggt
    13501 aatgttcaga ccctcggtta gcccctgcag atagcttact ttttcagaca tatcgcgcaa
    13561 cataaaaaat cctccttatt tttcactgtc tatagtatgg acagaataag gaggaaataa
    13621 acgatattca aaaaaactac gctctttcca tgtattgccc ggtccgggta tcaatgcgca
    13681 ggcgatcccc ttcgttgaca aagagcggca cctgcaccac ggctccgctt tccagtttgg
    13741 cattcttggt ggcgccggtg gcggtgtcgc ctttgatgcc gggttccgtt tcgacaaccg
    13801 tcagctcaac aaagttgggc agttcaatcc ccatgatggt gctgttgtgc atcagcaccc
    13861 cgatggtcat gttttccatc agccatttgg agccatcccc gatctcttcc ttggaaatgg
    13921 tcagctgctc ataattttcg ttgtccataa aaacatagcc gtcgccgtca ctgtagaggt
    13981 attgcatttc tcttttatcc aaatgggccc gggccacttt ttctccgccg cgaaaggttt
    14041 tttccaccga gccgccggtc ttgacatttt tcagcttggc tctgacgaaa gctgctccct
    14101 tgccgggttt cacatgctgg aaatcaacca cttgatagat cacatcttca aattcaatcg
    14161 tcaacccggt tttaaagtca tttactgaga tcatcgtatc cctccattat ataattaata
    14221 ggcctttatc tgaatgggtg atgacttcac atcccccttt cttcacgatt acggtatcct
    14281 caatgcggat tccgccccaa ccgggaatat agatgcccgg ttccacggtc actgccatgt
    14341 tttcctgcaa tatatcctga ctcgaggccg acaaacgtgg cgcctcgtgc actgccagac
    14401 ccagaccgtg gccggtgctg tgaacaaaat aaccgtccag ctgatatttt tgcaaatggg
    14461 cccggacttt ttggtccaac tcccgtccgg ccattcccgc ctgtaccagc ccgaccccca
    14521 gttcctgcgc ctccagcaac tgccggtagg catcgcgcaa tttggtatcc gcgtggtgaa
    14581 tggcgaccgt tctggtcata tccccggcat agccctgata caagccgccg aagtccatgg
    14641 ttagcatgtc tccgggctgc aatcggtaat cgcccggttg cccgtggggc agggcagcat
    14701 ttacaccggc tacggcaatg gtttcaaaag cttcccggct gcagccgcca ttttttaacc
    14761 cagtcacaat caggttggcg atctccgcct cctgcattcc ggccttgatt tgctgacaaa
    14821 ccgtggtgaa aacctggtcg ccgatgcggg cggcggttcg taacagctcc agttccgttt
    14881 cctctttgat gagccgcagc ttttccacca gcccctggac cggcagcaat tccggtttta
    14941 agccggcttg catctgcaaa aagcgatcat aggtaaggtc actgctctca aagcccagac
    15001 tggcaaaccc ttcgctcaag gcaatcaaaa cgtctgccgc gccgggcgtg gccttgacca
    15061 actcccagtc aggacattcc agcgatacct gggtataata acgggaatcg gtaatgatat
    15121 attttttgtc cagggtaatc agcaggcggg catcgcgtcc cccggtgaag cccgataaat
    15181 atcgaatatt ctccgggcag gtcaccaaca aggcttccat ccggtttgcc tgcaattccc
    15241 ggcgcaattt ttctacacgc tgtttgatac gtttcacctg ttcctcatcc ggcaaaaaac
    15301 tgctggattt attttaccct agtctcagga gatgtaaaat gtttttttca tgcatttact
    15361 taaaccattc acagatggcc aagagcgcga ggctgtatcc catggcaccc agaccgaaaa
    15421 taacgccgcg ggccaagggg gaaagcaggg aatgatggcg gaattcttcc cgggcacaga
    15481 tattggtcag atgcacttca ataaaaggaa tgccgactgc cttaaatgca tccatgaggg
    15541 caatgctgta atgggtcagc gcgccggcgt tgacgatcat ggcgtctacg ctgccccagg
    15601 cttgatgaat ctgatcgatg agttcgcctt catgattgga ctgaaagaag tcaatctcga
    15661 cctgcttttc ttctgccagc ttttttaagg catagttgat gtctgccagg ctcatgtcgc
    15721 catagagatt gttttcgcgg ttgccgagca gattcagatt cggtccgttg atcaccaaaa
    15781 ctcgcatatt gtcccacctt tctgtaatct ggttattttg agagtactac gaaagcacta
    15841 taactgtaag tctattctga agtatatgct tatgaccgct taagcatata cctctgcgat
    15901 tgatttcttt gcctttggag ccgggttttt gacttatcta attcgcatta tggtgcactt
    15961 tgttcattcc ttgaggcttt gccatttatt tatttaatcg ccgccgatgc tgatttggtt
    16021 gaaacggatg ctgggggcgg ctttggttcc gtagaagcgc tggtcgttgc ccagggcatc
    16081 aatattttgc agcagctggt gcagattgcc ggcgatggtg atgccgcgca cgggtctgga
    16141 cagctttccg ttttcaatca ggatgccggc agcacccagg gaaaaatcgc cggaaatggg
    16201 gttggcggta tgcatcccca tgacttcggt gattaaaaga ccgcgctcca gctcggcaat
    16261 catctgctcc ggcgatgtgg tgccgggttc tatcatcagg ttgctgctgc tgatggcggg
    16321 caggcttcgg aaggaaccgc gccggccgct gccggtggat tgcacaccgg ctttgccggc
    16381 gtagtaatgg tcaaagagga aggagcgcaa tgccccgtca cgaatcagtt cgttgcggcg
    16441 ggtgggtacg ccttcgccgt caaagggggc actggccagt ccgtccggca aggtgccgtc
    16501 atcgataagg gtgaggcgtg aagatgctac ctgttcaccc atgcgattgg cccacaggga
    16561 cttgcctttc agtacggcat cgccattgag cgacggcaac agcaggctca taaaacgggt
    16621 ggccacatag ggttccatga tacaaggcaa cgtctgcgaa gccatactct tggcctgcag
    16681 gctgcgcacc gcgcggttgg ctgcttcgcg accgcattct tgcggctgca gatcggccag
    16741 gcggcgacgg gccatggccg caaaaccgct ctgggattcc tgttcttcct gggcggccag
    16801 ggcgatataa aggccggcca gattgtcttg ataacttgcc tgtaaaccca agctgttcat
    16861 aatcaacgta aacgactggc tgtcttcata aacggatttc tccaccaaat ggatgcggga
    16921 atcgtaagcg cgggccgctt gctcgcaatc gcgggccagg gcaatttttt cttccagtgc
    16981 agcatccggg atctgcgcat caaaacagtc catctgcgca tagacagaat agggttcaac
    17041 cagcctttga tctgggtccg ccggcataca gttggagatg ttgaccgctt gctcgaccac
    17101 cgccaatacg gctgtcgggg acaggtcgct gctataggca aaacccattc gtccctggtt
    17161 gagcacccgc actcccactc ctatttcctc ggcctctttc aacgtctcaa cccgttcgtc
    17221 acgcagctca atgctcaatg acttgttgta gagcagaaag acttccgctg ccaccgcttt
    17281 ttcccgggcc gcctccagcg ccattgctcc tgcagctgtc aaactctctt ctgttacacc
    17341 gggacgttct ttttgcaaca ctttttccaa cactccttgt tacaccggga cgtttttttg
    17401 taacaccttt ttcagccgtc ctcagcggga tatgatccct acattctgcg aatgcgcttg
    17461 ttggtcttgg gattttcatt gttgccaatc ccacccacgg tcaatgtttt aatgcgaatc
    17521 gtcggctgcg catcagcaac aggaacgcct tgtccgtcct tgccgcacac gcccagtgaa
    17581 taacccaaat cccgtccaat ccggtcaatg tccagcagta cctgcggacc gttgccagtc
    17641 agagtggcac ctcgcaccgg atgcttgatt tctccctgtt caatcaggta gccttcctgc
    17701 acttcaaaga caaaatcccc attgagggtg ttcacctgtc cgccccccat ttttctgacc
    17761 agcaggccat actctgtccc ggcaatgatt tcctcgggat catcctgccc gggggcgata
    17821 aacgtattgg tcatacgcgg aatcggttta tgctgatagg attcccgccg gccgttgccg
    17881 gtcagggggg aaccgtcgtt gctggcggta agccggtcat tgataaattg ttttaaaatt
    17941 ccctgttcga tcatgaccgt acgctgcccc ggagtaccct catcatcgaa cccgaaactc
    18001 ccatatttgc ctttgagcgt ggcgtcgtca atgacactga gatgctcgtt ggccacccgc
    18061 tgtccctgtt tgccctgata aatggacaga cctttctgga ccagatcagc ctccaggcca
    18121 tgcccacaag cttcgtggac catggttccg ccggcttcgg catgcattac caccggcatc
    18181 ctgcccagcg gtgcaggcgc agcggagagc atcgttagcg cccggccggc cgcctcacgg
    18241 gccatgtcat ccagcgccac ttgctccata agctcccagc cgatagcccc tccggccgat
    18301 tcatagccgg tctggatcat cccgtcacgc gcggcaatgg cattgatcat catgcgcacc
    18361 cgcacctgtt cttcttccgc caacacccct tcggagttgg cgatctgcag ttttctctgc
    18421 acatccgcca atgctacggt gacctgtctg atttctgcat cgacctcccg ggccgcctga
    18481 ttggcagcca gtacccgggc gattttttca tcaaatgcta cttcgtcggg catgcgtaaa
    18541 taggccaggc tggcgcctga cttttgcggg gtgagattga tggcagcgtt ttcctgctgc
    18601 cccgcccgga acacccgggc tgccacctcc gctgcccttt ccaatccgcg gggactcaac
    18661 tcattggtat aaacataagc cgtccggcca tctttcagca cccggatgcc ggctcctttt
    18721 tcccgtccgc tgttgattct ttcaatgcgg ttgtcttcac agagcacatg gctgagctgc
    18781 ttttcttcca cgaaaatctc ggcaaagtcg ccgccctggc ggagcgctgt atccaagacg
    18841 cgggccagtt gttttttatc caacatcata aactcctttg gctgtgaatc aaatatttac
    18901 tgccttcatc gctcctcgcg atgatatggc aaggttaggg cggccggtgc acctgcttcg
    18961 gaacgcatcc gaatggttac gatgaccagc accagaatcg tcaacaaata cggcatcatc
    19021 gccagataaa acgtggaaat attcaccccg gccgtctgca tacggaaggt aagcgcttcc
    19081 accccgccga acagatagga accaagaatg gcccgcatgg ggttccaaag cgcaaagatg
    19141 accagcgcta ccgcaatcca tccccgcccg gaggtcatgt tttcctgcca ggaaggcgca
    19201 taggccagcg ataaataggc cccgcccata cccgccagaa tacccccgac gatcgtacaa
    19261 aaataacgca cccggctgac actgatgccg actgcatcag ccgcggcagg attctgcccc
    19321 accgacctga tactcagtcc ccagcggctg tggtagagca ccagccacat caggacggca
    19381 atgacaatgc tgagataaac caatgcatcc tgctggaaaa agatccgccc cagcaccgga
    19441 atgtcagcca gtccaggaat atggatggcc cgaaaaggat ccgaaatggg cgttccctga
    19501 taggatttgc ccaggaatcc gctcagcccg gtaccgaaaa tagtcagcgc cagaccgcat
    19561 accacctgat tggagcgcag ggtgatggtt aagaaggcat gaatcgccgc cattccgcca
    19621 ccgcccgcca tggctgccgc cattccgtac cagtggttgc cggtgcggac ggtaaaaata
    19681 aatcccaaaa ccgcgcccac cagcatcatg ccctcgacgc ccagattcat aattcctgat
    19741 ctttccgcca gcaattcccc ggacgccgca tataatatgg ctgtaccggc aacgacggtg
    19801 gccgccagca aagctacaat cgcttcaaaa gacattattg gccctccttt ccgctgcgct
    19861 gcaggtggta tttggtgaaa atctctccgc ccaacaggaa gaacaatacc gcaccttcca
    19921 gcatctgcac ggttgaaaag ggcaagccaa atatctgcag gccaaagccc cccaccagca
    19981 aggcgccaaa caaaaaggcc accatgatga ttgcaaacgg attcaagcgg gccagccagg
    20041 caacaatgat agccgtaaag ccatagccgg gagaaatccc ctgctgcagc cgatgggtaa
    20101 tgccgctgac ctcgatcatg ccggccagac cggcaatgcc gccgctaatc agcatgacca
    20161 gcaagatgtt gcgggtcaca ttgatgccgg tataacgggc ggaggcatgg ctttcgccga
    20221 tgactttaat ctcgtagccc catttggtgt aattgagcag gaaatacaac aaaaccgcga
    20281 ccagcaacgc taccagcagc ccgatatgca cccggctgtc accaatggaa ggcagatacg
    20341 cctggggcga aaagcgcgga ctcaacggaa aacccatact ggccggatct ttccagggtc
    20401 caaagataaa gctttccaca aaggaaatcg ccacataatt cagcatcagg gtggtaatcg
    20461 tctcattgac atcccaatag gctcgcatcg cccccggaac caggccccat atccctccgg
    20521 ccacaaaggc tgccaggccc atcaccggca agagcaccag cgaaggcaga tctccataaa
    20581 acaaggcaat gccgctggcg gcaaaggcgc ccatatacag ctgcccttct gcgccgatat
    20641 tccagagttt catgcgaaag gccatcgaca atcccagacc cagcaacatc aaggggatgg
    20701 ccttgaccaa tgtctcggaa atcgcatagc tgctgccgaa tgccccctgc cacatgcgca
    20761 gatacgtctc ggccgggtta atcccataca accacaggaa gatccagcca aacagcagtg
    20821 ccaaaacgat ggaaagcacc ggcacaataa aatgcgaagc caaggattcg gtttctcgtt
    20881 taataaattt atattggatc ataactgtgt ttcacctccg cccagcatca gcagcccgac
    20941 cgtttccaaa tccgcttgct gaatgtcaaa aaccgctttg atcgtaccgg catacatgac
    21001 cgcaatgcga tccgccaggc ggaaaatatc ctccaaatcc tccaggatga gcaaaatcga
    21061 ggtgcctttg gcccgttcct gcagcaacaa atgaaaaacc gattccgttg ctgccacatc
    21121 caatccccgc actggataag acgccaccag caaacgcggg tgctggctga tctcccgtcc
    21181 cagcaggagc ttctgtaaat tgccgcccga cagataattg atagggtgat tgatatctgc
    21241 cagcgccaca tcaaaatctt taaccaattt atgggtataa gcgcggattt ttttataatt
    21301 caataaaaat ccgcgggaat attccggttt actgtaatta tgtaaaattg cattttccat
    21361 cactgacagg ttggggacca gccccatgcc caagcgatcc tccggcacga tgttaatccc
    21421 ccgggccagc cgttcgcgta cgctcagcgc actgatttcc tgaccatcca tcttgatgct
    21481 gccacccgcc aggctgcgca atcctgcaat tgcttccacc aattcccgct gtccgttgcc
    21541 tgccacgccg gcaatgccga tgatttctcc gctgtacagt gacaggttta aatcctttac
    21601 ggccacattg ccctggtcgc ccaacaccat cagatgatcc agctgcagaa tcaattcccg
    21661 ggaacgctcc agtacctccc gccgacttgg ggtcagttcc ttgcccacca tcatctgggt
    21721 caggaccttt tcatccaaat ccgccgtccg cgcattgccg acgaccttgc cgcgtcgcag
    21781 cacggtaacg ctgtccgcca cctccaggac ctcattcaac ttatgggtga tcagtacaat
    21841 cgattttccc tgatccgcca tcttccgcat cattttaaag agttctttca cttcctgggg
    21901 cgtgagtaca gtggtcggct catccataat tagaatatcg catccccggt aaagcatttt
    21961 caggatttcc acccgctgct gttcccccag ggacaattgc gagaccttgg cctgcgggtt
    22021 gatttccagg ccatacttct ccgacaggcg ggcagcctct tcctcaatcg ccttgctgcg
    22081 caggacgata tctcccccgg atgcgcccag gataatgttc tccgccaccg taaagttatc
    22141 gatcagccga aaatgctggt aaatcatgcc cactccgacg gccagagcgg aacgcggcga
    22201 gcgaaagcgc accggcttgc ctttaatctt tatactgccg gcatcgggat gatataaacc
    22261 cgccagcaca ctcatgaggg tggacttgcc agccccgttt tcgcccaaaa gcgcatgtat
    22321 ttcgccggcc ctgacctgga aactaatcgc atcattggcc actacaccgg gaaatacttt
    22381 ggtgattcca ttcatttcaa tcattaaact gcttgtcaaa ctatatccct ccgctgataa
    22441 ggaaaccgtt cttgaggttc agatgtgaaa atattgcaga atccgatttt atggaaaacc
    22501 cggctggatt gccaggctcg tccgcggcta aaatttttag aggcggctaa aagccgcctc
    22561 taaactgtca taacaaattg gctactgacc tattttacca tctactccag cgacaaacca
    22621 gttgaaaccg agaatttctc cgtcggtcat cttctggcct tcctgtacct tgatggtacc
    22681 gtcctgtgcc ttcagcggac cggcaaagat gtccagtttg cctgacttga tatccgcctg
    22741 tttggccacg accagttctt tgacatccgc cgggacttta tccgaaatgg gagccagatc
    22801 gataattccg tcttctgctc ctccccagta tgcgccactc ttccaggtgt tattcatcac
    22861 tgattttaca aggtccacat agtagggacc ccagttccac acagaagcgg tcaggctgta
    22921 gttgggtgca tacgttttca tgtcactgtg atagccgatg gcaaatacgc cggcttcttc
    22981 cgccgcaatc tgcggggagg tggcatccac gtgctgggtg atgacatccg cgccgtcttc
    23041 aatcaaactg tcagccgcct gtttggccag gttggcatca atccagttat gagtccagac
    23101 gactttcacc ttgacattgg ggttcacgga ctgcgccccc agggtaaacg cattgattcc
    23161 acggacgact tccggaatat cataagctgc aacataaccc aaggtattgg atttggtcat
    23221 cttgccggcc acgattccgg tcagataacg catctgctcg atcttgccga aataagtgcc
    23281 gacattttct gccgtgttga tccccgaagc atgcatgaac accacgtcag gatacttttt
    23341 ggccacttcg atggtggcat cgccgaaacc aaagctggtg gtgcaaataa ctttacaacc
    23401 ctgttgggcc agctggtcaa tacttctttc cgcatcggcc ccttcggcta cgttctccag
    23461 gaaaagtgtt tccacttccg gaagttcctt ggccaggtat ttgcgaccct cgtcatgggt
    23521 gtaggtccat cctgcatcac cgggcgtgcc tatgtagacg aaaccaacct tgaatttttc
    23581 cttttccccc tcgggtgcct tgccggtatc cttggaagtg ccgcagccca ccacaaacac
    23641 tgacaggaac acaaacaaca ggatgaatga acctagtttc ttgaacttca ttaaaccaaa
    23701 atcccccttc taaatttttt tcgcgcttgc cgtaccgata aaacttcggc aggtacaacc
    23761 aaagtctttt acttcgacat tatatcaatt gaatcctgca atttaaacag caaaagctat
    23821 ttttatccag cgattcgccc tacgtccgcg ccgccaaacc caacgcaatc aggaaatcat
    23881 attttatatt cagtgtttct tcttcactga ggttggcagg caagacgagc tccggcatcg
    23941 ggtcggcaat ctcgacttcc aaaccgctgc cagaagccag actggcatca agccctttga
    24001 aacgggcacc tgcgccgcac agccagattt tttcaatcct ttcctcggtt ttgttctttt
    24061 gcagattata gtattccact gaacgagtaa catcatcaat cagatcccgg gtcaggtaat
    24121 catattgact cccctgtccg aattgaatat cctcccagcg aacagcaccg ttgggaaagc
    24181 tcatctccag cgccgcataa aaagcggaaa tgccaaaagc cagggagcga taaaagacca
    24241 gtacatcatt tttaaatacc gaaaagaagg aacgtgtccc gccaatgttc agataggcat
    24301 tgacgtcttc cggatacaat ttgcgcaata cccggttcat ggccaatggc tcgatctcca
    24361 ccgccaccag gttcaaaccg gcgatgttgc agaccatgtc caggttctcc acctgctggc
    24421 gccgcaccgc cacgaagaag acttcggttt tcttgccttc ctcgtcttca aaatcccgca
    24481 gcgggaagat gtcgatggcc gcctcctcga ccgggatggg caggaaattg atggcctgaa
    24541 aagaaatggc ttctttcagt tcatccaatt tcatccgcgg catgaccaat gtacgggtat
    24601 aaacctgctg accggccact gctgagacca ccttgtgatg ctttattttt aatttactca
    24661 ccagtttgcc cagttcttcc cccaatccga caggatcaac aatgttgccg gactcaacca
    24721 tccccggcgg tgtatccatg ctctcaaacc aaaccacctg aatcccttgt ttggtattct
    24781 tgacccgcgc cagtttaatt ttcttgctgc cgatatccaa tccaatgccc tgcgaaccgc
    24841 caaacaattg tatcaattca acacacccca ttcagacaaa tttatttatc tgcccccggt
    24901 attctggtga aaaccatctc tcgtcatatg ctgcttaact tcgcttgcag tcgagttaat
    24961 tcctctaatt gggaggaatt atttatatgg atgtaaatca attgtatatg attttataat
    25021 ccgtgataac cccattgtct gaataagtgc gggacagaaa cgcctgatcg ttatcgcagt
    25081 tgttggcata atggaaagta aggtctgcca ccaccgtttt ggtggcgttt tcaaagcaac
    25141 cggtaaactt gatggtatag cagcagtagc cgtcaccggt ggccagtttc acaaaagagg
    25201 tctggcaacg tccctgcaca tcaccgacag gcatttccat ccaatcctcc cgccatccca
    25261 gcggcagcgg aggcaggtct ggcgtgatcg tataatcgct gcgcaaacac tgactgatct
    25321 ctgcgcagcg ctgttccaga catgcatcga ccagttgctg ggcttgctgg ctgcgataat
    25381 aatactggct catatacatc tccagggccg acaattgcat gatcgacatc atcaggatac
    25441 tgaccataaa aaaaatgagc aggctcatta acaacatgct gccctgctcc ctggctgtgc
    25501 ttttcttttg acacggcaat gcatccgcct cccggcagga gattcgaacc gtacctgctc
    25561 cgcttgccat catttgtctg actcagccgg ggctgccccc accagcgagc gaggaactgc
    25621 cgcgcagacc aggcgatggg tcttcagtcc ctgacaggcc tccatgctga tgcgcgtgta
    25681 tcccgggcgg gaatcaggct gaaacaacag actgctgata tcattggcca ctggcatctt
    25741 ggcgttcccc tggcgataga ccgtccggtc ctgcaaataa taggaaatgt agccgccatc
    25801 catcgtcagg cgcatggtct tgctgtcatc caggcattcc acctcctcgg ccgccatgac
    25861 atcctcaatc atcatctgca tcgccgcccg ctcggcatat tgaatatccg taaacgccgt
    25921 ttgacaggca aaaacccggt aggcactgaa atacaccgtc atcaaggcgg caatcaccag
    25981 cgttgccagc cccaatgcca gaatgatctc ggccattatc aagccgttgt catcaaacct
    26041 gtgctgcatc tctatccccc cgcaaagccg gataaaaaag cgttatcatt tccagcgaac
    26101 gattctctcc cgcttcttgc caggtcacaa cgacccgggc atcataaagc cccggccagt
    26161 caccgttttc ttccaggcaa accgtcgcct gcatctcctg acctgcctct gcagtcccat
    26221 ccaccgtata atgatgtgtc ccctgttcca ctgctgttat gatttcctgc cactgcggac
    26281 aagactttgc ccgcagatcc tccaacaact gataggcaaa actgctggcc cgggtctgtc
    26341 gttcagcata ctttatcata cgctgggaaa tcatgaagcc ccccagcaaa ctgctgctga
    26401 tcaaggccac caaaaacaag gtcaccatca cttccaccag cgtcgtacct ttttccctgt
    26461 gccatccgtt catcacaacc gccgcccatc aggatttgat gtcataatta tagcttattt
    26521 tatctttaaa gggaatattt gttatat
//
LOCUS       DAOTHZ010000026        25937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2181_length_25937_cov_7.035971, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000026 DAOTHZ010000000
VERSION     DAOTHZ010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 25937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25937
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_25937_cov_7.035971"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="P5549_04745"
     CDS             complement(<1..708)
                     /locus_tag="P5549_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRW12411.1"
                     /translation="MIKRYTLPAMGEVWSEASKLNNWLQIEVAACEAWAQLGKIPVEA
                     VETIKARARFDVERIAAIEVEVRHDVIAFLTNVGEYVGDDAKYIHLGLTSSDILDTGI
                     AMQMRDAADLILAKLEKLQAAIKGKALQYKYTLCVGRTHGVHGEPTTFGLKMALWYTE
                     MDRNIERLKQAREVIAYGAISGAVGNFAHLDPRVEEYVCQKLRLKACPVSTQVIQRDR
                     HADFMTNLAVGASSLEKM"
     gene            complement(705..1229)
                     /gene="purE"
                     /locus_tag="P5549_04750"
     CDS             complement(705..1229)
                     /gene="purE"
                     /locus_tag="P5549_04750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRW12412.1"
                     /translation="MPQVGIIMGSDSDLAIMKDACDMLDQFAVTYDVTVCSAHRLPHE
                     TAAYATTAETRGVDVIIAGAGGAAHLPGVIAAYTILPVIGVPIKTSTLSGLDSLYSIV
                     QMPKGIPVATVAINGSANAALLALEMIALKDEALKHRLTEYRHKMGSEVLDKNQRLQE
                     MGVQEYLQQKEGGQ"
     gene            1647..2381
                     /locus_tag="P5549_04755"
     CDS             1647..2381
                     /locus_tag="P5549_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRW12413.1"
                     /translation="MQQNKNYAEVLKHTIGMHREPVAVKLVGEEAQLSHINPNDYDAG
                     TKSRYCQGVMRATRGERVILNADNISCAAAAAAFGFKTLHPKLASGEAHFNTGTFGTQ
                     EAAQRIMGDMPRLACGDYHSVLISPLALAEFEPDVVLIEAPPENLMWLALACIYTSGE
                     RLQFSTSVVQATCVDCTVVPFISGKTNATLGCNGCREATDLNVTENLLGIPYASLSSI
                     IDNLANMKEFITKNRSKSIYERFDKK"
     gene            2410..3102
                     /locus_tag="P5549_04760"
     CDS             2410..3102
                     /locus_tag="P5549_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRW12414.1"
                     /translation="MTSNTQSVQAQLFELQDLKYKAFQCKLMPTVNPDTVIGVRTPDL
                     RKFARAFSQTPQAEEFIHILPHTYYEENNLHGFLIEMIKDYNAAIAAVEAFLPYIDNW
                     ATCDLISPKVFKKNLPGLYEKIKVWLKSSQTYTVRFGIGMLMSFYLNDEFQPEMLELV
                     ASVRSQEYYINMMIAWYFATALAKQHDAALSVLQEQRLEKWTHNKAIQKSIESYRIDD
                     DVKAYLRSLKVK"
     gene            complement(3207..3557)
                     /locus_tag="P5549_04765"
                     /pseudo
     CDS             complement(3207..3557)
                     /locus_tag="P5549_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3726..4529)
                     /locus_tag="P5549_04770"
     CDS             complement(3726..4529)
                     /locus_tag="P5549_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRW12415.1"
                     /translation="MCLIIFAYDCHPKYRLVVAANRDEFYQRPTQPAAFWPEHEGLLA
                     GRDVKEGGTWMGVTTGGRFATLTNYRDPASFNPSARSRGHLVYNYLAGEAKPLEYIAS
                     MISQADLFNGFNLLLGDQESLYCYSNRDHVLRKVESGIHGLSNSVLDDPWPKLAKGKH
                     TLAGIMADKVLDVERLFTMMADREQPSDEMLPQTGVSLEMERVLAPAFVISPQYGTRT
                     TTVLLIDRAGQVQFRERSFAVGQTDKWNEVHYEFETDYRGQSPKITRQD"
     gene            complement(4665..5498)
                     /locus_tag="P5549_04775"
     CDS             complement(4665..5498)
                     /locus_tag="P5549_04775"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRW12416.1"
                     /translation="MFTIGCHLSAAKGYAHMGKEAVSIGANTFQFFTRNPRGSKAKAI
                     DPKDVEKFLQFAREHSLIKILAHAPYTLNACAAEPRTREFALEVMADDLLRLEHTPNN
                     YYNFHPGSHVGQGVERGIELIADVLNQILKPAQTTMVLLETMSGKGSEVGGKFEEIGQ
                     IIDQVQLQEKLGVCLDTCHIFDAGYDIIHDLDGVLAEFDRAIGLHRLQAIHLNDSKNP
                     FASHKDRHEKIGEGTLGLDTIIKVINHPQLKHLPFYLETPNELEGYAEEIKLLKAAYQ
                     E"
     gene            complement(5533..6567)
                     /locus_tag="P5549_04780"
     CDS             complement(5533..6567)
                     /locus_tag="P5549_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRW12417.1"
                     /translation="MIFFNNDYCEGAHPLVMEKLLATNLEQTIGYGEDHHCMEAARLI
                     RKKCGRDDLDVHFLVGGTQTNFTFIASALRPHQGVISADTGHINVHETGSIEAVGHKI
                     LALPSPDGKITAAQVRQVCEDHYGDSTHEHMVQPKLVYISNPSEVGTIYSRAELTALH
                     ETCRQNDLILYMDGARLGYGLCSPENDLDLPAIARLCDAFYIGGTKVGLLFGEALVIC
                     DERLKPDFRYIIKQKGGMLAKGRLLGVQFAALFTDDLYFAMSRQAIALAMQIKAACTR
                     KGYQFLIDSPTNQQFPILPDAMLERLSENFVYSYWQRVDDRHSAVRFCTSWATLPENV
                     EQLIAAIEKS"
     gene            complement(6622..7884)
                     /locus_tag="P5549_04785"
     CDS             complement(6622..7884)
                     /locus_tag="P5549_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRW12418.1"
                     /translation="MLKRIVDRNKFRFLLIISLSLLWIVVYLMRVNIGALLVDARFLQ
                     DLALEGKTAQQGLLMTVFLVVYSLANMAAIPISKRLGPRQTIVLGVLIAGLAMIAGGW
                     AASFAAILLIRVLLGVGNGIHFPNMSTLVKRWFPPQERGMANSLYGVGGCIGMVIAFP
                     LFSAINNHLGWEYSFIIPGLLALLATLPLWLRWISDSPGDNPYISVTELNYICSWNRE
                     ADGEQNEKEGFGVRLLLGNPSFWMLCLAYTAFLSSWWGLLTWMPQYLVEARGFNLQGT
                     ASNVTLAYIVSTIGILTGGRLVDRSSRRSRIAFMSLMTVALATLGVAVIPSASLAVIF
                     MIVAVGINEFVYPCVWSLLQSLLPDRMIAAGSGTMSGVSNLLSALSPLAIGWLIQISG
                     SYVLGLMFLVSMSAVGALSCLFLYRQGY"
     gene            8180..8785
                     /locus_tag="P5549_04790"
     CDS             8180..8785
                     /locus_tag="P5549_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12419.1"
                     /translation="MILGIDIDNTITHTTEMILHYAQIFGREQGLNTVADLRYYYLED
                     ILGWDKRVADDFLNHYLGRIYTNMQPKEQAVEVIRELKKQHELILITSRNQKFPAVEE
                     VTRNWLQQHGVPYDRLILNQTDNMHFFSKLEVCRDNGVEVMIEDHYELVSEISPHLPV
                     IMFEYPYNRHLHNENIIRVNHWQEVAGWLEQYRPQQMNSAG"
     gene            8843..10237
                     /gene="asnS"
                     /locus_tag="P5549_04795"
     CDS             8843..10237
                     /gene="asnS"
                     /locus_tag="P5549_04795"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRW12420.1"
                     /translation="MDKLLVRQLVRDFTAYTGREVELHGWIRNNRDQKSFGFIALNDG
                     THFNTIQIVYDKERLRNYNEVAKYRVGAAVIVRGVVVNTPEAKQPFEISAREILLEGD
                     AAEDYPIQPKKHSREFLRETAHLRPRTNLFAAVFRIRSVLAYAIHKFFQERGFVYVHT
                     PIITAIDAEGAGEMFRVTTLDDQHPPLREDGEVDFSRDFFGSRTNLTVSGQLQAEVFA
                     LAFRDVYTFGPTFRAENSNTARHAAEFWMIEPEMAFADLQDDMRMAEAMIKYLIAYVL
                     EHCSQEMEFLNSFVDKGLKERLLQVVQAEFARITYSEAVDLLLQSKATFEYPVAWGHD
                     LQTEHERYLTEKIFNKPVFVTDYPQKIKAFYMRLNDDGKTVAAMDLLVPGVGEIIGGS
                     QREERYAMLQQKMESFHINEKDLWWYLDLRKYGGVKHAGFGLGFERILMYITGVTNIR
                     DVIPFPRTVRSCEF"
     gene            complement(10355..11389)
                     /gene="mutY"
                     /locus_tag="P5549_04800"
     CDS             complement(10355..11389)
                     /gene="mutY"
                     /locus_tag="P5549_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRW12421.1"
                     /translation="MQPWVKMLLSWYDQEKRDLPWRRCRDPYAIWISEIMLQQTRVET
                     VRDYYRRWMERFPDIKSLAQADVDTVLKMWEGLGYYTRARNLHKAARLMVEASDGNFP
                     SSYLEILALPGIGSYTAGAVASIAFDQPTPAVDGNVLRVMSRLFALPDIARPDVQAAI
                     KKEVAEGFPDQRAGDYTQALMELGALVCVPRRPLCERCPLTGLCQAFAQNAQGLWPLV
                     KEKKPGREVKRLVAVIRQGDAILLHRRQPTGLLAGLWEFPGVEGSRKQELAVRFAEEF
                     GMQLKVGQHVLDAIHVFTHLQWQMKVYEATLVGDADLSGQPDFKWVRIDEIEQLAIPT
                     AFQKIKQRLQ"
     gene            complement(11521..12342)
                     /locus_tag="P5549_04805"
     CDS             complement(11521..12342)
                     /locus_tag="P5549_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451552.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRW12422.1"
                     /translation="MKRNIKTLYFSATDTTKKVVTELAGSLAARMNGAAGISQFDFTL
                     PEARQVVRSYAGDDVVVVGVPVYAGRVPNVLVKYLHTLQGNGALAVAVVVYGNRDYDD
                     ALLELTDLLTEQNFTVIAGGAFVGEHSFSRTLGAQRPDEKDIAIVRDFAGQIYSKLTT
                     QTSFPPIAVKGNHPYKKYYAPRDKQGNKISDFHLIKPKTSDDCIDCKLCAEVCPMGSI
                     DYDQPSVMIGICIKCCACIKKCPVEAKYFDDENYLWHKQLLEENFARRSEPEFFV"
     gene            complement(12390..12860)
                     /locus_tag="P5549_04810"
     CDS             complement(12390..12860)
                     /locus_tag="P5549_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase P"
                     /protein_id="HRW12423.1"
                     /translation="MIDLSIPGREPMQLQQLILDMNGTLTKDGVLQEGVAERIEQLKG
                     HLKIYLLTADTFGTGAQVARHLGIRISVVSSANGVQDKSDFAASLVPAGIIAVGNGYN
                     DKEMFAQADLSIAVIGPEGCCVKALLNADIVVNHINDALDLLLNPLRIIADLRT"
     gene            complement(12920..13762)
                     /gene="modD"
                     /locus_tag="P5549_04815"
     CDS             complement(12920..13762)
                     /gene="modD"
                     /locus_tag="P5549_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HRW12424.1"
                     /translation="MYISEDTLDRIIKEDTPYIDLTTMVLGIGNEQGRISFSSREAGI
                     LCGSEEVARILQKLGAKTNLVLPSGTSISPQQLFLQAEGTAAGLHIAWKASLNIVEYC
                     SGIATRTHELLTKARTVNPDVNILSTRKMFPGTRELAVKAVVAGGGLPHRLGLSETIL
                     VFRQHINFLGGMEELAKNIPQFKQRDCEKKFIVEVEKLDDALLLAAAGVDGLQFDKVP
                     PQQLKQFVEVIRESYPGIVLIGAGGIKESNVIDYAASGVAGIVTTSMYFGRPLDISVK
                     IEPI"
     gene            14099..15943
                     /locus_tag="P5549_04820"
     CDS             14099..15943
                     /locus_tag="P5549_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12425.1"
                     /translation="MTVRIPEDLNFIKFLSTGPLMVYVLNEEGKYVWTNKALQEFTGF
                     GAEELLNLENFWEPLVHPEFQEMLKSNHYARSRGEDRPDNYEIKIKLKDGTEKWVDLF
                     VTIEMIEGHPYTFFGAYDITARKMAQMELQMAHDHLDHLVQERTQEVTLSNHALTASQ
                     IMLQSVISNISDGVIILTSQGEIEFINRKLEYMLGNTLKTLKDQLIFEVLTSRNPSIK
                     QLFMKQQAFAEEEVSLQINGREMQFLATGTPIEDSDGLGNKGILILKPLAEIHRLVNR
                     YNSAQAHFRFADILTDDPIMLDTIEAARNAALNKNTILIEGESGTGKELFAQSIHNQS
                     VCADGPFIAVNCGAIPHELVGSELFGYADGAYTGARKGGNPGKFELAQGGTLFLDEIS
                     DMPLEQQVALLRVLQEKQICRLGGSKTIPVDVRVICATQKHLFTEVQKGTFRQDLYYR
                     INVLNLRIPPLRERHGDIELLLNYFLTSGDKQWLEHMNGIEASVWNLLKNYAWPGNVR
                     ELQNFAEKILYSVKDYAIRTENLPREILDAFSQHPNHHSITATPLPAALSSAQLPSSI
                     KQQKAALERSQIKALLKQYNGNVGRVAADLGVSRRTVDRKINLYNIER"
     gene            complement(16081..17703)
                     /locus_tag="P5549_04825"
     CDS             complement(16081..17703)
                     /locus_tag="P5549_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW12426.1"
                     /translation="MDRRWYQFYDQEVPPSIDYPNITLKDMFNSNAEANPDKPYLIFK
                     DIKIPYKVSNSMARQLANALLSVGVEKGDRILLMMPNIPQYAISLMACYKIGGIAVGA
                     NPLYTVPELTHQFSDCSAKAVIVLASFAEKALQIYQEGKTQIEQVIIVGAKGSGIETN
                     DHIHDYDTLMAAGSHEEPAIEVVPEDIAMLQYTGGTTGVSKGCMLTHANLVAMTMQDG
                     IWFSPSVPPEVIRTLATIPLHHIFGMNCNINLSLYGGGTIVLVAQPTPDNILEAINDH
                     EPTCWAAVPALIQGMNQHPGIRDSKIKSMKGICCGSAPLPVEVMKTFVEISGAMITEG
                     YGMSETSNIVTANPIKGLKKTGSIGIPWPDVDIKIVDVETGTKEMATGESGEILIKGP
                     QVMKGYWNNPQETANVFRDGWLYSGDVGYMDEDGYIFIVDRKKDMLLCSGFNVFPREI
                     DEVMYTNPKVLEACTIGVPDEKRGETVKVFVVLKPGQSMTEEEVIDFCKQQLAPYKVP
                     KIVEFIDSVPRTSVGKPDRKALRAMEEAKRNG"
     gene            complement(18082..19011)
                     /locus_tag="P5549_04830"
     CDS             complement(18082..19011)
                     /locus_tag="P5549_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRW12427.1"
                     /translation="MAGIMIYSDKTAYALQLVTVAQMIAKDAGLAIKAISINDAEQAG
                     ELSKRADTYKIDNAAIQVGDTAAVAAALKQTAAQLGANIVLLSSNRRGKELSGRLAQS
                     LGAGCLTDVNSISSNAGKIECSRNALGGATVAVQQIETENQVIAIMPKAYEAAPEAGG
                     SLNDLAVDVKASPLKVLSVNPKGGDSVDIEAADILVAVGQGVENKDDLATVEAIAKAL
                     GGEVACSKPVATDKKWFSEERIIGLSGKKCKPSLAILLGISGQVQFTVGIRDAKTIVA
                     VNNDENADILKMADYVMVADVEEVMPALLKALS"
     gene            complement(19017..19772)
                     /locus_tag="P5549_04835"
     CDS             complement(19017..19772)
                     /locus_tag="P5549_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRW12428.1"
                     /translation="MNIVVAMKQIPDLQQVRIRNRQPVFEDVPLTLGNIDKNALEAGV
                     TLKEAADAKLFVVSAGNEELEDTVKEALAAGADEAFLVIDDALDHISSDVSAALIAGA
                     VKKIDAVGIILFGEGSGDNYSGQVASRVAEILDLPQIGYAKAIELADNKVKVVRSLED
                     CEEVVESELPVVISVVSDINEVRIPSVTQILKAGKKPKEVVEPDDLDIAINAKPIVAT
                     LSNLAPESARKGIAVKSVDELVKALTTEGLIGR"
     gene            complement(19948..20232)
                     /locus_tag="P5549_04840"
     CDS             complement(19948..20232)
                     /locus_tag="P5549_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW12429.1"
                     /translation="MSELMGLKAKLGLDVFKHDKEPHIKIKPGMEKDARLKKAVLVCP
                     AGLYSENAAGEVELTIDGCLECGTCRIACGTEVLEWNYPNSGCGVQFRFG"
     gene            complement(20229..21515)
                     /locus_tag="P5549_04845"
     CDS             complement(20229..21515)
                     /locus_tag="P5549_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW12430.1"
                     /translation="MEKDFDVIVVGGGLSGLAAAYTMADAGMEVLVLERGDYSGAKNV
                     TGGRLYVNPVREMFPDLWKKAPLERFIAHEEVSIMDQDRSITMRYDGNELAQEPYQSY
                     SVLRGKFDKNFAKVTERKGVSMVTKSRVDDLIIENGKVEGVMAGEDALTANIVIACDG
                     VLSLLAEKAGLRKPGLDKDHAVGFKEIIELDRTVLEDRFNLEGDEGAARLYMGEVTRG
                     KFGGGFLYTNKESISLGIVVGIKDLMEEPLVQAPDLLDAFKQRPEVARLIKGGQTSEY
                     SAHVIPEGGLHSMGKLYGEGILIAGDAAGFSMNIGVTVRGMEYAMATGYYAAQAAIKA
                     KEAGSYNAATLAVYEKLLSESFVMKDFQSFKEAPHALDNPRFFNRYPQLAGDIMRDLY
                     AVPNGPKERIYPTMMKYIKFNEMFAFLKDFWGVRKI"
     gene            complement(21613..22752)
                     /locus_tag="P5549_04850"
     CDS             complement(21613..22752)
                     /locus_tag="P5549_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW12431.1"
                     /translation="MDFSLTKEEALIQKMAREFAEKNILPIAEQIDHENKVPQEIIEG
                     LAELELMGIPFDEKFGGANAGYVSYVLAMEQLARCSSGVTMILSAHTLAVSSINTFGT
                     EEQKLKYMPKCCNGEFIASFAFTEPGTGSDPKQITTTATKDGDSYVLNGTKRFISNAN
                     YPGPIVIFARETETNEITAFIVDKWSEGYSISEPWKKMGLHGGLLLDVYMKDVRVPAS
                     NVLGQAGQGYPILQLGISFGKIGVSSSSLGGILAAYEEGMKYAKEKTHRGTPIAKFQS
                     IQLKVADLAMLLESSRWLTYRLAYLADNFKNPIVFAKEAALTKTVVCDNSVKAARVAL
                     DIHGSYGCMEDYKISRLYKDAIMGPQIEGVEDMQKMIIAGVLLKG"
     gene            23258..23677
                     /locus_tag="P5549_04855"
     CDS             23258..23677
                     /locus_tag="P5549_04855"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRW12432.1"
                     /translation="MRCMGIDAMNALSVTTKNTDLKYGTSYHENFLTYLQFFQDNNIV
                     ASCAQTDSKGNRRYRPGEQPDPDQYLHVVERRPDGVVVRGTKLHNTMSPYVDEIIAIP
                     TRALLKNVNRLCHRLSDSRCTWGRLTHYGRYRDHGCL"
     assembly_gap    23704..23803
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24062..25609)
                     /locus_tag="P5549_04860"
     CDS             complement(24062..25609)
                     /locus_tag="P5549_04860"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRW12433.1"
                     /translation="MPYLCISLFITCILVTLLFFTWKNRTVLGARYFLLTLLLAEFWI
                     AGQALEMAALDLATKIFWANLQYIPITLISISYLYLALQFTGHERWLKSRWLVMILLV
                     VPIVVNILAWSNDFHGLVRQNVFLDTSGAFPTVGKTYGPGFWAFAVYNYVIASFTLTI
                     LAMGFREKISLYRKQLSLLFIALLLPVISNVFQVTGMNPFHVDITPPVIGLAALIISW
                     GIFRYRLFDVVPIAHSIIIEEMRTGMIILDREGRFLDVNPAARKMLDLPAKDFSGHFL
                     ETELGKLPALIEIFKQGKDAVGEVAFGSDKIPNYYEVSFTLVKNEKNEFIGWLLQIYD
                     ITERKVAEGIIRHAAYHDALTGLPNRQYFHLLFAKELAFAKMVADVLTVAFLDLDDFK
                     IVNDTWGHMAGDQVLCEVAERLKGSLRESDIISRIGGDEYAIVLPHRGNVETFETIEA
                     KIQEALRPSIELQDGAVQISASIGFSMFPHDADNIEDLLKLADKAMYEAKQKKEANCQ
                     YRRTCKA"
     gene            complement(25713..>25937)
                     /locus_tag="P5549_04865"
     CDS             complement(25713..>25937)
                     /locus_tag="P5549_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12434.1"
                     /translation="NCHLPSGKSNLRKTIDGTKFQRAAGGKGKNALQYPGAKKSMFSG
                     RENVSRETLLPVKCLQYVFGSGNGMRKIFS"
BASE COUNT         6574 a         6730 c         5923 g         6610 t
ORIGIN      
        1 cattttctcg agggaactgg cccccactgc cagattggtc ataaaatcag catgacggtc
       61 ccgctggatg acctgggtgg aaaccggaca tgcttttagt cgcagctttt ggcagacata
      121 ttcttcaacc cgtgggtcga gatgggcaaa attgccaacc gctccggata tcgctccata
      181 agcaatcact tcccgggctt gctttaatcg ctctatattc ctgtccatct ccgtatacca
      241 gagtgccatt ttcaacccga aagtggtggg ctcgccatgg actccgtgcg tgcggccgac
      301 acacagggtg tatttgtact gtaaagcttt tcccttgata gcagcctgga gtttttccag
      361 tttggccagg atcaaatcgg ctgcatcccg catctgcatt gcaatgccgg tatccagaat
      421 atccgaagag gtcaggccga gatggatgta tttggcatca tcgcccacat attcgccaac
      481 gttggtaaga aaagcaatga catcgtggcg gacttccact tcgatggctg cgatccgctc
      541 tacatcgaag cgagctcggg ccttgattgt ttccaccgct tccaccggga tcttgcctaa
      601 ttgcgcccaa gcttcacagg cggcaacttc tatttgcagc cagttgttga gcttgctcgc
      661 ttccgaccag acctccccca tggccggcag cgtataccgt ttgatcattg gccgccctcc
      721 ttctgctgca gatattcctg cacacccatc tcttgaagtc gttggttctt gtccaaaacc
      781 tcactgccca ttttatggcg gtattcggtg agtctgtgct tgagcgcctc atctttgagg
      841 gcgatcattt ccaaagccag caacgccgcg ttggcactgc cattgatggc taccgtggcc
      901 accggtatgc ctttaggcat ctgcacgatc gaatagaggg aatccagccc ggagagggta
      961 ctggtcttga tgggtacacc gataaccggc aagatcgtat aagcggcaat cacacccggc
     1021 aagtgggcag cgcctccagc gccggcaatg atcacatcca ctccccgggt ctcagcagtg
     1081 gttgcatagg cagccgtctc atggggcaga cggtgggcgg aacagacggt tacgtcatag
     1141 gtaacggcaa attggtcgag catatcacag gcatccttca tgatggccag atccgaatca
     1201 cttcccatta tgatcccgac ttggggcatg cttttcatcc tcctttttgt ccggtaaatg
     1261 tatacagtat ttataaagcc cagacagata ggcttcgtaa ccgaaacctg tccgcctggg
     1321 cttttatccc tatacggtgt agtgccagag gcacctgtgt cacttggacc tgtccacggt
     1381 tatgcgtgcg gaaccctaga cacactttcg tgttatatgg ttattatgtc catatagatt
     1441 gatattagca gagctttcat ggggtgtcaa ctcgaagcac gaacatcagg aaatacgagt
     1501 atgtaatcgg ttcaatcctt gacttaacgt tctcctaaat actatattta atttgataat
     1561 gaacatatgc tctgaaaaaa acagtttgtg aaagaaacaa ttttgatgaa aaacttttga
     1621 taattaacaa tacgaggggt gtttatatgc agcagaataa aaactatgcc gaagtactca
     1681 agcatactat tggcatgcac agggaacctg tagccgtaaa acttgtaggc gaagaagcgc
     1741 aactcagcca tattaatcca aatgactatg atgccggtac caagtctcgc tattgtcagg
     1801 gcgtaatgag agcaacacga ggggaaagag ttatcttaaa cgctgataat atatcctgtg
     1861 ccgctgccgc tgcagcattt gggttcaaaa cccttcatcc caaactggct tcaggtgaag
     1921 ctcatttcaa tacgggtact tttggaacac aggaagccgc ccaaagaata atgggggata
     1981 tgcccaggct tgcatgcgga gattatcact cggttctgat atcacctctg gctttggcag
     2041 agtttgaacc agatgtcgtt ttgattgaag cacccccgga aaatttaatg tggctcgctt
     2101 tggcctgtat ttataccagc ggtgaacgtt tgcagttttc cacttccgta gttcaggcca
     2161 cttgcgtaga ttgtacggta gttcctttta tcagcggaaa aacaaatgcc accctcggct
     2221 gtaacggatg ccgtgaagca acggatttaa atgtcaccga aaatctgctc ggcattcctt
     2281 atgcttccct atcttctatc atcgataatc ttgccaatat gaaagaattc attacaaaaa
     2341 atagaagcaa gagcatctac gaacgctttg acaaaaaata actgtaaaca agaagccagg
     2401 agatagaaaa tgacgagtaa cactcaatcg gttcaggccc agttgtttga gctgcaggat
     2461 ctaaaatata aagcgtttca gtgcaaactc atgccgaccg ttaacccgga tacggtcatc
     2521 ggggtgcgca ccccggatct gcgcaagttt gcccgggcat tttcgcaaac gccgcaagct
     2581 gaagaattta ttcatatcct gccgcacacg tattatgaag aaaacaacct gcacggcttc
     2641 ctgattgaga tgatcaagga ttataacgcc gccattgcag cggtagaggc attcctgccc
     2701 tacatcgaca actgggccac ctgtgacctg atttcaccga aggtatttaa aaaaaatctg
     2761 ccggggttat acgaaaaaat aaaggtctgg ctgaaatcca gccagactta tacggtacgt
     2821 ttcgggatcg gtatgctgat gagcttttat ctgaatgacg aatttcagcc ggagatgctt
     2881 gagctcgtag ccagtgtacg ctcccaggaa tactacatta atatgatgat cgcctggtat
     2941 ttcgccaccg ccctggccaa gcagcacgat gccgctttgt cggttttgca ggaacagcgc
     3001 ctggaaaagt ggacccataa caaggctatt caaaaatcga ttgagagcta tcgcatcgac
     3061 gatgatgtca aggcctacct gcggtcgtta aaagtgaaat gaaaccgtta aaaatttaat
     3121 catatcttta tgcaaaatta tattgccttc cagattttcg ttgccgccac gcccgcctac
     3181 ccaggtgtaa accctcgaac tgtcagtcaa tacttgtact tcaaacgaat taaacattgg
     3241 aataaattcc gtgttgattt ttgattgagg agcaatcgaa aataattcgt taaatatttc
     3301 atcttgccgg catagccttc aaaggtgtca aacctggaaa gccctccggg atagaaggga
     3361 ctctgtataa cttttacgtt ggtggatttg cgaaactcga ttgaagaaat atgatgatgc
     3421 ttcttgaaag cagaactgta attggacgga ctgtatccgt aatccaatcc aatatcggtg
     3481 atcggtttat cctttaccag cttgatatcg acggcgctct ggtccatctt cagacgtttt
     3541 atgaatccgt acacactttc accggttacc tccttgaagg accggctaaa gtaatattcc
     3601 gagaaatgaa gatgatcgga acatcctgca ccgaaatatt ctcgttaaaa tgctggataa
     3661 tataatcaat actccggttt gtcagttcct tattaatcat aactttttct tttgctttca
     3721 tcattttaat cctgccgagt aatttttggg gactgtcccc gataatcagt ctcgaattca
     3781 taatggactt cgttccactt gtcagtttgt ccaaccgcaa agctgcgttc ccgaaattga
     3841 acctgaccgg cacggtctat caataaaacg gtggtagtgc gagttccgta ttggggactg
     3901 attacgaagg ccggcgccag cacccgttcc atttccagac tcaccccggt ttgaggcagc
     3961 atctcatcag acggctgttc ccggtcggcc atcatggtga acaatcgctc gacatcaagc
     4021 actttatccg ccatgatacc tgccaaagta tgtttaccct tggcaagctt gggccagggg
     4081 tcgtccagta cgctgttgct gagaccgtgg atgccgcttt ccactttacg cagcacatgg
     4141 tcccgattgg aatagcagta tagtgattcc tggtcaccca gcagcaggtt gaaaccgtta
     4201 aacaagtctg cctgactgat catggatgca atgtactcca gcggtttcgc ttcgcccgcc
     4261 agatagttat acaccagatg gccgcgcgac cgggccgagg gattgaaaga agccggatcg
     4321 cggtagttgg tcagggtggc aaatcgcccg ccggtggtca cgcccatcca ggtgccgcct
     4381 tcttttacat cacgaccggc cagcaaccct tcatgttccg gccagaaagc tgccggctgg
     4441 gtgggacgct gatagaattc atcccggttg gcagccacca ccagcctgta ttttggatga
     4501 cagtcatagg cgaatatgat cagacacatg ttgattgctc cattttcatt tatttaggtg
     4561 tttttcatca tttagaaagt caggcaatcc tgttcgctta cggtgacatg ggttattaaa
     4621 attacatgat gcaagttcct tgcttcatgg tctgccgttg agccttattc ctgataagca
     4681 gccttgagca gtttgatttc ttcggcatag ccctccagct cattgggcgt ttcgagatag
     4741 aaggggagat gtttgagctg ggggtggttg atcactttga ttatggtatc cagccccaag
     4801 gttccttccc caattttttc atggcggtct ttgtggctgg caaaggggtt tttgctgtca
     4861 tttaaatgaa tggcctgcag ccggtgaagc ccgatggccc tgtcaaattc cgccaatacg
     4921 ccgtccaaat catgaatgat atcgtagcca gcgtcaaaaa tatgacacgt atccaaacac
     4981 acgcccagtt tttcctgtag ttgcacctga tcgatgatct gcccgatttc ttcaaatttg
     5041 cctcccactt cactgccctt gccggacatg gtttccagta ataccatagt ggtctgcgct
     5101 ggttttaaaa tctggttgag cacgtcggcg atcagctcga tccccctttc cacgccttgc
     5161 ccgacatggc tgccggggtg gaagttgtag taattgttag gggtgtgttc caagcgcagc
     5221 aggtcatcag ccatgacctc cagcgcaaac tctctggttc ttggttcggc cgcgcaggcg
     5281 ttcagggtat agggcgcatg ggccaggatt tttatcaaac tgtgctcacg ggcaaattgc
     5341 agaaattttt ccacatcctt cgggtcaatg gccttggcct tgctgccccg aggattgcgg
     5401 gtaaaaaact gaaacgtatt ggcaccgata ctgactgctt ccttgcccat atgggcatag
     5461 cctttggcgg cagacagatg acagccgatg gtgaacatgc tttgcgcctc cttgcttggc
     5521 tgctgacagc cgttatgatt tttcgatggc agcgattaat tgttccacgt tttccggcaa
     5581 ggtcgcccag ctggtgcaaa agcgcaccgc gctatgccga tcatccaccc tctgccagta
     5641 ggaatagaca aagttttcac tgagcctttc cagcattgca tcgggcagga tgggaaattg
     5701 ctgattggtg ggagaatcga tcaggaactg atagcctttc ctggtgcagg cggctttgat
     5761 ctgcatggcc agtgcaatgg cctggcggct catggcaaaa tacaaatcat ctgtaaacaa
     5821 agccgcaaac tgcaccccca gcaaccgccc cttggccagc ataccaccct tttgttttat
     5881 tatgtaacga aaatcaggct ttaaccgctc atcacagata accagcgcct cgccaaacaa
     5941 caagcccacc ttggtcccgc cgatatagaa cgcatcacaa agccgggcaa tggcaggcag
     6001 gtccagatca ttttccggtg aacacagtcc ataacccagt cgggccccat ccatgtaaag
     6061 gatcaggtca ttttggcggc aggtctcatg caaggcggtc agttccgccc ggctatagat
     6121 ggtgcccacc tcggaagggt tggaaatata gaccagtttg ggctgaacca tgtgttcatg
     6181 ggtggaatca ccataatggt cctcacaaac ctggcggacc tgggcggcag ttatttttcc
     6241 gtcagggctg ggcaaagcaa gaatcttgtg cccgaccgcc tcaatggatc cggtttcgtg
     6301 cacattgata tgcccggtat cagcggagat aaccccctga tgcgggcgca aggcagaggc
     6361 tataaaagta aaattggtct gggtgccgcc caccagaaaa tgcacatcga gatcatcgcg
     6421 cccacatttt ttcctaatca gccgggcggc ttccatacag tgatgatcct cgccgtaacc
     6481 aatggtctgc tccaggttgg ttgccagcaa tttttccata accagaggat gggccccttc
     6541 gcaataatca ttgttgaaaa aaatcatcag ccaattcctt ctttacaaac ttttttaagc
     6601 tgcaacgcca agcagccggc cttaatagcc ttgacgatac aagaacaaac aactgagcgc
     6661 ccccaccgcc gacatggata ccaggaacat caagcccagc acatagctgc cgctgatctg
     6721 gatcaaccag ccgattgcca gaggcgatag ggcggaaagc agattgctga cgccgctcat
     6781 ggtcccggag ccggcggcta tcatccggtc cggcagcagt gattgcaaca gcgaccagac
     6841 acaaggataa acaaactcat tgatcccaac ggcaacaatc ataaagatca ctgccaggga
     6901 ggccgaaggg atcacagcca cccccagagt agccagcgcc actgtcatca gggacataaa
     6961 ggcgatccgg ctgcggcggc tgctcctgtc caccaggcgt ccgccggtca aaatcccgat
     7021 ggtggaaacg atatatgcca gtgtaacgtt gctggcggtg ccctgcaaat taaacccccg
     7081 ggcttccacc agatattgcg gcatccaggt cagcaacccc caccaactgc ttaaaaaagc
     7141 cgtataggcc aggcacagca tccagaagga gggattgccc agtaataatc tgacgccgaa
     7201 accctctttt tcattttgtt caccatctgc ttcccgattc caggaacaga tataattcaa
     7261 ttctgtgact gatatatagg gattatcccc aggtgaatcc gaaatccagc gcagccacag
     7321 cggcagagtc gccagcaagg ccagcagccc cgggatgata aagctgtatt cccaccccag
     7381 atggttattg atggcggaaa aaagcggaaa ggcgatcacc ataccaatac agccgcccac
     7441 cccgtaaaga gaattggcca ttcccctctc ctgcggcgga aaccatcgtt taaccaaagt
     7501 gctcatattg gggaaatgaa tcccgttgcc gacgcccagc agcacccgaa tcagcaggat
     7561 ggcggcaaag gatgctgccc agccgccggc gatcattgcc aggccggcga tcaatacccc
     7621 cagcacaatg gtctgccgcg gccccaatcg tttactgata gggatggcag ccatgttggc
     7681 cagcgagtag acgaccagaa atacggtcat cagcagtccc tgttgggcag tcttgccctc
     7741 cagcgccagg tcctgcagga aacgggcatc aaccagcagg gccccgatat tgacccgcat
     7801 cagatagacc acaatccaga gcagacttag ggaaattatc aataaaaatc ggaacttgtt
     7861 gcggtcaaca atccttttta gcaatctttc atcttctttg cataaatgat tggggcagga
     7921 atctctccct gactccagca tttattgtag cataataaac aggttgcagc gctataaatg
     7981 aaagcaggga aggaagaagc aggagagatt tcattcgttg caactcttca acaagttcag
     8041 aatgtgacag gaatagtatt ttctttttca gcctatcgac cctgcttgga cacatcagga
     8101 agacattagc acttgccagc cagtcaaatt ggttttaaga tgaaacgata gtctgttaga
     8161 aaaatcagga ggatataaga tgattttagg cattgatata gataacacca ttacccatac
     8221 caccgaaatg attctgcatt atgcgcagat tttcggccgc gagcaagggc ttaatactgt
     8281 tgccgacttg cgatattatt atctggaaga catcctgggc tgggataaaa gggttgcgga
     8341 cgacttttta aaccattatc tgggccggat ctataccaat atgcagccca aggaacaagc
     8401 cgtggaagtc atccgggaat taaaaaaaca gcatgaactg atcttgatta cctcccgcaa
     8461 ccaaaaattc ccggcggtcg aagaagtaac ccggaattgg ctgcaacagc atggggtgcc
     8521 ctatgaccgg ttgatcctca accaaactga caatatgcac tttttcagca aactggaggt
     8581 ttgccgggac aacggggtgg aagtgatgat cgaggaccat tatgaactag tcagcgaaat
     8641 cagtcctcat ttgccggtaa ttatgtttga gtatccttac aaccggcacc tgcataatga
     8701 aaacatcatc cgcgttaatc actggcagga agtggccggc tggctggagc aatatcggcc
     8761 gcagcagatg aacagcgcag gctaatcaat taataattgc cgggtatatg gcgtaatata
     8821 aatagaacga aggaggatgc ccatggacaa actactcgtg cgtcaactcg tcagagactt
     8881 caccgcatat accggcagag aggttgaact ccacggctgg atcagaaaca atcgtgacca
     8941 aaagtcattc gggtttattg ccctaaatga cggtacccat ttcaacacca ttcaaatcgt
     9001 ctacgacaag gaacgtctga gaaactataa cgaagttgcc aaataccggg tgggggcagc
     9061 cgtaatcgtc cgaggcgtgg tcgtaaacac gcccgaagcc aaacagcctt ttgaaatatc
     9121 cgcccgcgag attctgctgg aaggggatgc agcagaggat tatcccattc aacccaagaa
     9181 gcattcccgc gagtttctgc gtgagacggc tcatttgcgg ccgcgcacca acctgtttgc
     9241 cgccgtgttt aggatcaggt cggtcttggc ctatgccatt cataagttct tccaggaacg
     9301 aggctttgtc tatgttcaca cccccatcat caccgccatt gacgcggaag gggccggtga
     9361 aatgttccgg gtcaccaccc tcgatgatca gcatccgccc ctgcgtgaag acggggaagt
     9421 agacttcagc cgggactttt ttggcagccg gaccaacctg accgtcagcg gccagctgca
     9481 ggccgaagtg ttcgcgctgg ccttccggga tgtctatacc tttggtccca ccttccgggc
     9541 cgagaattcg aataccgccc gtcatgctgc cgaattctgg atgattgagc cggaaatggc
     9601 ctttgccgat ctgcaggacg acatgcgcat ggccgaagcc atgatcaaat atctcatcgc
     9661 ttatgtattg gagcattgtt cgcaagagat ggaattcctc aacagctttg tggacaaagg
     9721 tctgaaagaa aggctgctgc aggtcgtcca ggcagagttt gcccgcatca cgtatagcga
     9781 agcggtggat ctgctgctgc aatccaaagc cactttcgaa tatccagtgg cctggggcca
     9841 tgatctgcaa accgaacatg agcgctatct gacagaaaag atatttaaca aacccgtctt
     9901 cgtcactgat tatcctcaga aaatcaaggc tttttacatg cgacttaatg atgacggcaa
     9961 gaccgtggcc gccatggatc tgctggttcc gggtgtgggt gagatcatcg gcggcagtca
    10021 gcgggaagaa agatacgcaa tgctgcagca aaaaatggag tcatttcata tcaacgaaaa
    10081 agatctgtgg tggtatctgg atctgagaaa atacggcggg gttaagcatg ccggctttgg
    10141 attgggattt gagcggatcc tcatgtacat caccggggtc accaacatcc gggatgttat
    10201 tcccttcccc cgtaccgtcc gctcctgcga attttaatcc aattcgttgg tatgccgcta
    10261 cacatcacaa ccccttttat cttgaataaa cgcatgctca taggaatgta aaaagctgtc
    10321 ccttaaccac gataggttat agaagacagc tttcttattg cagtctttgt ttgatttttt
    10381 gaaaggcggt ggggatagcc agttgctcga tttcatctat gcggacccat ttgaaatcag
    10441 gctgcccgct gagatcagca tcacccacca gggttgcctc atagactttc atctgccatt
    10501 gcagatgggt aaagacatgg atggcatcca acacgtgttg gcctactttc agttgcatac
    10561 cgaattcttc cgcaaacctg accgccaatt cctgcttccg gctgccttcc acgccgggga
    10621 attcccataa accggccagc aatccggtag gctgtcgccg gtgcaggagg atggcatcac
    10681 cttggcggat gaccgccacc aaccgtttga cttcccgtcc cggctttttt tccttgacga
    10741 gcggccatag tccttgggca ttctgtgcaa aggcctgaca caaacctgtc aacggacaac
    10801 gttcgcacag ggggcggcgc ggcacgcata ccagcgcgcc caattccatc agcgcctgag
    10861 tgtaatcacc cgcccgttgg tcagggaagc cctctgccac ctctttttta atggctgcct
    10921 gcacatccgg tcgagcgatg tccggtaggg caaagagacg gctcatcacc cgcagcacat
    10981 tgccgtcgac ggccggcgtc ggctggtcaa aggcaatgct ggcaacggcc ccggcggtat
    11041 aggaaccgat ccccggcagc gccaggattt ccagataaga cgaaggaaaa ttcccgtcgc
    11101 ttgcttccac catcagccgg gcagctttat gcaggttgcg ggcgcgggta tagtaaccca
    11161 ggccttccca catttttagt acagtatcga catcagcttg tgccaggctc ttaatatccg
    11221 ggaaacgctc catccaacgc cggtaatagt cccgcaccgt ttccacccgg gtctgctgca
    11281 acatgatttc cgagatccag atggcgtagg gatcgcggca gcgacgccag ggaagatcgc
    11341 gcttttcctg atcgtaccag cttaacaaca ttttcaccca gggttgcatg attattgctc
    11401 cttcgaagag tacgtttccg tttttttcct ggctggtcct tggccaataa agtgcgcaga
    11461 caaaattccg gattttttcc aggatggcgg ataagccacg ggtaatgttt attactgtta
    11521 ttacacaaaa aattccggtt cgctgcggcg ggcaaaattc tcctccagta gctgcttgtg
    11581 ccataaatag ttctcgtcat caaaatactt ggcctcgaca ggacattttt taatgcaggc
    11641 acagcacttg atgcagatgc cgatcatgac ggaaggttgg tcataatcga tggaacccat
    11701 gggacaaacc tctgcacaaa gtttacagtc tatgcaatcg tcgctggttt taggtttgat
    11761 gagatgaaaa tcggaaatct tattaccctg tttgtctctg ggggcatagt attttttata
    11821 cggatgattg cctttgaccg caatgggcgg aaaggaagtc tgcgtggtca gcttggaata
    11881 aatctgcccc gcaaaatcac gcacgatggc gatatctttt tcgtcaggac gctgggcgcc
    11941 gagggtgcgg gaaaaggaat gttctccgac aaaggcgccg ccggcgatta cagtaaaatt
    12001 ctgctcggtc agcaggtctg tcaattccag cagcgcatca tcataatcgc ggttgccgta
    12061 gacaaccacg gctaccgcca gggcaccgtt gccttgcagg gtatgtaaat atttcaccag
    12121 cacattgggc acccgtccgg cataaaccgg cacacctacc acgaccacat cgtcgcctgc
    12181 atatgagcgc actacttggc gcgcttccgg cagggtaaag tcaaattggc tgatgccggc
    12241 agcgccgttc atcctggctg caagactgcc ggccaattca gtgaccacct ttttggtggt
    12301 atcggttgca ctgaaataca gtgttttgat atttcttttc attccctcaa ctccccgtaa
    12361 acagtcattt tttcgtttag tccaataaat cacgtacgca gatcagcaat aattcgcaat
    12421 ggattaagca gtaaatccaa ggcatcatta atatgattga caacaatatc cgcattcaac
    12481 aatgccttta cgcaacaacc ctccggcccg atcacggcaa tcgacaaatc tgcctgtgca
    12541 aacatctcct tgtcgttata gccgttgccg acggcaatga taccggcagg aacaagagaa
    12601 gcagcaaaat cagacttgtc ctgcacgcca ttggcagaac tcacgacaga tatcctgata
    12661 cccagatgcc ttgctacctg tgccccggtt ccaaaagtat cagcggtcaa cagataaatc
    12721 ttcaggtggc ctttcaactg ctctatccgt tcggcaacac cttcctgcag aacaccgtct
    12781 ttggtcagcg tgccgttcat atccaggatt aactgctgca gctgcatagg ttctcttccc
    12841 ggaatcgaca aatcaatcac taaatcccat ctccttccgt ccacgcccgc ttcgtagctg
    12901 accaagtcat gatcacggtt tagattggct caattttaac cgatatatcc aacggccggc
    12961 caaaatacat ggaagtcgta actattcctg ccacacctga agccgcataa tcaatcacat
    13021 tggattcttt gattccaccc gctccgatca gcacaatacc aggatatgat tcccggatca
    13081 cttctacaaa ttgcttgagc tgctggggag ggaccttgtc aaactgaagt ccgtccactc
    13141 ccgctgccgc cagcaggaga gcgtcgtcca gtttttctac ttcgacgatg aatttttttt
    13201 cgcagtcccg ttgcttgaat tggggaatat ttttggccag ttcttccata ccgcccagaa
    13261 aattaatgtg ctggcgaaag accagaatgg tttccgacaa ccccaggcga tgaggaagcc
    13321 ccccccctgc gactaccgct ttaacagcca gttctcgtgt ccccggaaac attttgcggg
    13381 tcgacaatat gttgacatca ggatttaccg tgcgcgcttt ggtcaatagt tcatgggtgc
    13441 gagtagcgat gccgctgcag tattctacta tattaagact ggctttccaa gcgatatgca
    13501 aaccagccgc agttccctcc gcttgcagga aaagttgctg cggtgatatt gacgtaccgc
    13561 tgggcagcac cagattggtt ttggccccca gtttttgcag gatgcgggcc acttcctcgg
    13621 atccgcaaag aattcccgcc tcacgggagg aaaaagagat tcttccctgc tcattaccaa
    13681 ttccaagtac catcgttgtc aaatcaatat aaggcgtatc ttcttttatg attcggtcca
    13741 gtgtatcctc actgatatac atattattac ctccgtacaa aagagttgaa caagacatcg
    13801 tctttatttt agacaatgat tacaggaatt atatcattaa tacaagcata tgcccacaat
    13861 aaattaattc tgttacatct gcagcccgtt atccgttttt tctgccaatc agattctttt
    13921 ttaaatactc cggataaatg tctggccagc gaatcccatc ccgaagtctt tcaagacaaa
    13981 tatgactcaa catgatgata attggccaat ttttcgacaa tactgtccat gtttgtttgt
    14041 ctgcaatcgt atgcttgtgt tatattttga acaacaggct tgagggagga aatgttgcat
    14101 gaccgtcaga attccagagg atttaaactt tattaagttt ctcagtaccg gaccgctaat
    14161 ggtttacgtc cttaacgaag agggcaaata cgtatggacc aacaaagcgc tgcaggaatt
    14221 taccggattc ggcgcggaag agttgttaaa cctggaaaac ttttgggagc ctttggtaca
    14281 tccagaattt caggaaatgt taaagtcaaa tcattatgcc cgctcgcgcg gtgaagaccg
    14341 accggacaat tacgaaataa aaatcaagct gaaagacggc acggaaaaat gggttgatct
    14401 ctttgttact attgaaatga tagaaggaca tccttacacg ttttttggtg catacgacat
    14461 caccgcaagg aaaatggccc agatggagct gcaaatggct cacgatcatc tggatcacct
    14521 ggtccaggaa aggactcagg aagtgacctt gtcaaatcat gcgctgaccg ccagtcaaat
    14581 tatgctccaa agcgttatct cgaatatttc tgacggcgta atcatcctta cttcacaagg
    14641 agaaattgaa ttcataaacc gaaaactcga atatatgttg ggcaacacct taaaaacatt
    14701 gaaagatcag ttaatttttg aagtattgac atcgcggaac ccctctatca aacagctatt
    14761 catgaaacaa caggcctttg ccgaagaaga ggtgtcatta cagatcaatg gccgggaaat
    14821 gcaattcctg gctaccggaa ctcccattga agattccgac ggacttggca acaagggcat
    14881 cttgatcctt aaaccccttg ctgaaataca ccggctggta aacagatata acagtgccca
    14941 ggctcatttt cgctttgcag atattctgac cgacgaccca atcatgctgg atacgatcga
    15001 agcagccaga aacgcggctc tcaataaaaa taccatcctg atcgaaggcg aaagcggtac
    15061 cggaaaggaa ctgtttgccc agtccatcca caaccagagt gtctgcgccg atggcccgtt
    15121 cattgcggtc aattgcggcg ccatccctca tgaactggtc ggcagcgaac tatttggata
    15181 tgccgacggg gcttacaccg gtgcaagaaa aggtggaaat cccggcaaat ttgaacttgc
    15241 ccagggcgga acattattcc tggatgaaat atccgacatg ccgctggagc agcaggttgc
    15301 tctgctgcgc gtcctgcagg aaaaacaaat ttgccgactt ggtggctcga agaccattcc
    15361 ggttgatgtg cgcgtgatct gtgccacgca aaagcatttg tttacagaag ttcaaaaggg
    15421 aacattccgg caggacctgt actatcggat caatgtactc aacctgcgta ttcctcccct
    15481 gcgtgaacgc cacggagata tcgagttgtt gttgaactat tttctgacct ccggtgacaa
    15541 gcaatggctg gagcatatga acggcattga agcctcggtc tggaacctgc taaaaaatta
    15601 cgcatggccg ggcaatgttc gagaactgca gaattttgca gaaaagatct tgtattccgt
    15661 caaggattat gcaatccgaa cagaaaatct tccgcgggag atcctggatg ctttcagcca
    15721 gcatccgaac catcattcga tcactgccac cccactgccg gctgctctgt cgtccgccca
    15781 gctgccatcc agcatcaagc aacaaaaagc cgcgctggag cgcagccaga tcaaggcatt
    15841 gctcaaacaa tacaacggca atgtcggccg tgttgcagcc gatttgggag tctcacgccg
    15901 caccgtcgac cgcaagatca acctgtacaa tattgaacgc taggctccta aacattcggt
    15961 cccgtcttta tactatgaaa acacgctccc ctttccgcag gcggactgct ttcaggggag
    16021 catttctatg tgcacgcatc ttcaggtttt taccattaaa aaacaatttg tcgtacaacc
    16081 ttagccgttt cttttggctt cttccatggc gcgcaaggcc ttgcggtctg gtttgcctac
    16141 cgaagttctc ggcacactgt caataaactc aacgatcttc ggtactttgt aaggtgccaa
    16201 ctgctgtttg cagaagtcga taacttcctc ttcggtcatg ctctgaccgg gtttgagaac
    16261 gacaaacacc ttcaccgttt cgccgcgttt ttcatccggc accccaatcg tacaggcttc
    16321 caacaccttg ggattggtgt acatgacttc atcgatttcg cgcgggaaaa cattgaagcc
    16381 gctgcaaagc aacatgtcct ttttgcgatc cacaataaag atatatccat cttcgtccat
    16441 ataaccgaca tcacctgaat acagccagcc atccctgaat acattggcgg tctcttgcgg
    16501 attattccaa tatcccttca tcacctgggg tcctttgata agaatttctc cagactcacc
    16561 tgtggccatc tctttggtcc cggtctcaac atcaacgatc ttgatgtcaa catccggcca
    16621 gggaatacca atactgcctg tttttttgag tcccttgatg gggttggcgg taacaatgtt
    16681 gctggtttct gacatcccat aaccctctgt aatcatggca ccggatattt ccacaaatgt
    16741 cttcattact tctaccggca gcggcgctga tccacaacaa atccctttca tggatttgat
    16801 ttttgaatcc ctgattcctg gatgctgatt cattccttga atcaaagccg gaactgccgc
    16861 ccagcaagta ggttcatgat cgttgatggc ttccaaaatg ttgtccgggg ttggctgtgc
    16921 caccaaaacg atcgtacccc ctccatacaa tgaaagatta atattgcagt tcattccgaa
    16981 gatgtgatga agcggaatgg ttgccagtgt acggattact tcaggcggta cagagggaga
    17041 aaaccatatg ccatcttgca tggtcatcgc gacaagattg gcatgggtca gcatacagcc
    17101 tttggaaaca ccggttgttc caccggtgta ctgcagcatg gcaatatctt cgggaaccac
    17161 ttcaatggcc ggttcttcgt gactgccggc cgccataagg gtgtcataat catgaatatg
    17221 atcattggtt tcaatgccgc tgcccttggc accaacgatg attacctgct cgatttgggt
    17281 tttcccttcc tgatagatct gcagggcttt ttccgcaaat gaagccagca caatgactgc
    17341 tttggcactg caatcgctga attggtgagt cagttccgga actgtataaa gcgggtttgc
    17401 acccaccgct atcccaccga ttttataaca ggccatcagg gaaattgcat actgcggaat
    17461 gttgggcatc atcaacaaga ttctgtcgcc tttttccacc ccgactgaca acaaagcgtt
    17521 ggccaattgc ctggccatgc tattgctcac cttgtaaggg attttgatat ccttaaaaat
    17581 aaggtacggc ttgtctgggt tcgcttcagc attgctgtta aacatatcct tcaatgtaat
    17641 atttggataa tcaatgcttg gcggaacctc ctgatcatag aattgatacc atcttctgtc
    17701 cattctctac acctccgatt gtatttgtga gccttgtgtg tatagcctca ccatttctgt
    17761 ttctctctat taattagcaa attccatacc acatctgctt ttaaaaaaag agtcacaaaa
    17821 caagcctcaa cccggcaagc aatatttgtt ggcaagtttg atttaagtca attttgtctc
    17881 gacgaagtat tccttgaccg ttttaggaca tatttgtccc gtttgtttct ttatctttga
    17941 atgtagtgca acgatcattt tagatgaatg tattttttgc atatcatgta aacacaaact
    18001 ccaataaaaa gcctccggtt gatgcttttt cagtatcaac cggaggcttg tatcgattga
    18061 aatataaaga accggttctg cctaactcag cgctttcagc agggccggca tgacctcttc
    18121 cacatcggct accatgacat agtcggccat cttcaggatg tcggcatttt catcattgtt
    18181 gaccgctacg atggttttgg cgtcacggat accgacggtg aactgcacct gtccagagat
    18241 ccccaagagg atggccaggc tgggtttgca tttcttgccg gaaagaccga tgattctttc
    18301 ttccgagaac catttcttgt cggttgccac cggtttggag caggctactt cgccgcccag
    18361 agctttggcg atggcttcca cggtggccag atcatccttg ttttctacac cctgtccaac
    18421 cgctaccaat atatcagccg cttcgatgtc tacgctgtcg ccacccttgg ggttgactga
    18481 cagcaccttg agcggtgaag ctttgacgtc tacagccaga tcattcaaac tgccgccggc
    18541 ttcgggcgca gcttcatagg ctttgggcat gatggcgatg acttggtttt cagtctcgat
    18601 ctgctggaca gcaaccgtgg caccgcccag ggcattgcgt gaacattcga tcttgccggc
    18661 gttgctgctg atgctattaa catcagtcaa acagccggct cccaatgatt gggccaatct
    18721 gccggaaagc tccttgccgc ggcgattgga tgagagcaac acaatattgg cacccagctg
    18781 ggcggcggtt tgtttcagag cggctgcgac cgcagcagtg tcgccaacct gaatggccgc
    18841 attgtcaatc ttgtaagtat ccgcccgttt ggacagttcc ccggcctgct cagcgtcatt
    18901 gatgctgatg gctttaatgg ccagacctgc atccttggca atcatctggg ctaccgtaac
    18961 caattgcagg gcataggctg tcttgtcgct gtatatcata attcctgcca ttgtcatcac
    19021 ctcccgataa ggccttcagt ggtgagggcc ttgaccaatt cgtcaacaga tttcacggcg
    19081 atccctttgc gggccgactc cggcgccaaa ttgctgaggg tggcaacgat cggtttcgcg
    19141 ttgatggcaa tatccagatc gtcgggttca acgacttcct tcggcttttt gccagctttc
    19201 aggatctggg taacggaagg aatgcggact tcgttgatat cggatacgac cgatatgacc
    19261 acgggcagtt ccgattcaac cacttcttcg cagtcttcca gggaacgtac cactttgact
    19321 ttgttgtctg ccaactcgat ggccttggca tagccgatct gcggcagatc aagaatctca
    19381 gccacccgtg aggcaacctg accagaataa ttgtcaccgc taccttcgcc aaacaggatg
    19441 atgcccacag catcgatttt tttcactgca ccggcaatca aagcggcgct gacgtcactg
    19501 gagatatggt ccagggcgtc gtcaatcacc agaaatgctt cgtcggcacc ggcggccaga
    19561 gcttccttga cggtatcctc cagctcctcg ttgccggctg aaaccacaaa caatttggca
    19621 tccgctgctt cttttaacgt caccccggct tccagagcgt ttttatcgat gttgcccagg
    19681 gttaagggca catcttcaaa taccggctga cgattgcgga tcctgacctg ctgaagatcg
    19741 gggatctgct tcatagcaac cacgatgttc atttttttcc ctcctagcaa ttcattttat
    19801 atttactctc cgtatgcacc gatacaacca gggatggata cttcattatt attagcacta
    19861 atcatataat tcgcatccct ttgcatcaaa aaaaccaaac caactgagag ccactccata
    19921 agttagaaag tttggtgcct ggcaccttta tccaaaccgg aattgaacgc cgcatccgct
    19981 gttgggatag ttccactcca gaacttcagt cccgcaggcg atcctgcagg taccgcactc
    20041 caggcaacca tcgatggtca gctcgacttc tccggcagcg ttttcgctgt agagtcccgc
    20101 cggacagacc agcacagctt ttttcagacg ggcatccttc tccatgccgg gtttgatttt
    20161 aatatgtggt tccttgtcgt gtttaaatac gtccaggccc agtttagcct ttaaacccat
    20221 cagttcgctc atatttttct aaccccccag aagtccttga gaaaggcaaa catctcattg
    20281 aacttgatat acttcatcat cgtcgggtaa attctttcct tgggaccatt gggaaccgca
    20341 tacaagtccc gcataatatc cccggccaac tgcggataac gattaaagaa acgggggttg
    20401 tccagcgcat ggggggcctc cttgaagctc tggaaatcct tcatcacaaa actctcgctg
    20461 aggagttttt catacaccgc cagggtagcg gcattgtaac tgccggcctc cttggctttg
    20521 atggccgcct gggcagcata gtagccggtg gccatggcat attccatccc ccgcacggtt
    20581 acgccgatgt tcatggaaaa accagcggcg tcaccggcaa tcagaatacc ttctccatac
    20641 agcttgccca tgctgtggag cccgccttcg ggaatcacat gggcggaata ttcactagtc
    20701 tggccgcctt tgatgagacg agccacttcc ggtctttgtt tgaaggcatc cagtaaatcg
    20761 ggagcctgca ccagcggttc ttccatcaaa tccttgatgc cgaccacaat ccccagactg
    20821 atgctctctt tattcgtgta gaggaacccg ccgccgaatt ttccgcgggt gacttcgccc
    20881 atatacagac gcgccgcccc ttcgtcgcct tccaggttga agcggtcctc taaaacagtc
    20941 cgatccagtt caataatttc cttaaaacca accgcgtggt ccttgtccag acccggtttt
    21001 ctcaaaccgg ctttttccgc cagcaaagac aatacgccat cacaggcaat cacgatattg
    21061 gccgtcaggg catcttcgcc ggccatgacc ccttctacct tgccgttttc aatgatcaga
    21121 tcatcgaccc ggctcttggt gaccatggac acgccttttc tttccgtcac cttggcaaaa
    21181 tttttatcga atttgccccg caggacactg tagctctgat aaggttcctg cgccagttca
    21241 ttgccgtcat agcgcatggt gatactgcga tcttggtcca taatgctgac ttcttcgtgg
    21301 gcaatgaacc tttccagagg cgctttcttc cagaggtcag gaaacatctc ccgtaccgga
    21361 ttaacataca gtctcccccc ggtaacattt ttggctccgg aataatcgcc ccgctccagc
    21421 acaagtactt ccattcctgc atcagccata gtataggccg ctgccaaacc tgacaacccg
    21481 ccgcccacaa caattacatc aaaatccttt tccatcccgg gcacaccttt cttctcaaac
    21541 tagtgaccag tcctttttaa aaagggaacc ctctttaagg gttcccatat tcgcttcttt
    21601 ataatataag ctttaccctt taaggagaac tccggcaatg atcattttct gcatgtcctc
    21661 aacgccttcg atctgcggac ccatgatggc atccttgtaa aggcgggaga tcttgtaatc
    21721 ttccatgcat ccgtaagaac cgtggatatc cagggcaacc cgggccgctt tcacagaatt
    21781 gtcgcagacc accgtcttgg tcagggcagc ttctttggca aacactatgg gatttttgaa
    21841 attgtcggcc agataagcca gccgatacgt aagccaacgg gatgattcca acagcatggc
    21901 cagatctgca accttcagct gaatcgactg gaatttggcg atcggtgtgc cgcgatgggt
    21961 tttttccttg gcatatttca tgccctcttc ataagccgcc aggatgccgc ccagagaact
    22021 ggagctgacc ccgattttgc cgaaggaaat acccaattgc agaatcggat agccctgacc
    22081 ggcttgcccc agaacattgg atgcaggaac ccgcacatct ttcatatata cgtccagcag
    22141 caagccgccg tgcagaccca tcttcttcca gggctcggag atggaatacc cttcactcca
    22201 tttatccacg ataaaggcgg tgatttcatt ggtttcggtt tcacgggcga aaattacgat
    22261 cggaccgggg taattggcat tggaaataaa gcgtttggtc ccgttcagga cataactgtc
    22321 accatctttg gtggcggtgg tggtaatctg tttggggtcc gagccggtgc cgggttcggt
    22381 aaacgcaaag gacgcaatga attccccatt gcagcatttg ggcatgtatt tcagcttttg
    22441 ttcctctgta ccgaacgtat tgatggaact cactgccagc gtatgggccg acagaatcat
    22501 ggtaacaccg gatgaacaac gtgccagttg ctccatcgcc agcacatagc tgacatagcc
    22561 ggcgttggca ccaccgaact tctcgtcgaa gggaatcccc atgagctcca gctcagccag
    22621 gccttcaata atttcctggg gtactttgtt ctcatggtcg atctgctcgg cgatgggaag
    22681 aatatttttc tccgcaaact cccgcgccat cttttggatc agggcttctt ccttggttaa
    22741 actaaaatcc attctatatc gctccttccg ctaacatgtg ttagacaaat ttttgattcg
    22801 aatattttgt atcagaatta atttggtgtt tttttattat atcacaacct tatgatgctg
    22861 caattatttt cagcaaaagg ttttgatctt ctacattcca ttattataag atttgctatt
    22921 taagtcaaat tccctgctta aaataaagaa aaattcgctg gctcttgtgt tcaggcaatt
    22981 cattgtcatg ttacattatg tcattcgata cgttacaaac tgatccaatt taaaataatc
    23041 tttcgacgta tcacaatcat cccaacaatc agacgatacc gcttgtcgcc agttgattcc
    23101 cttcgtttct ggcatttcca gccttgacgg ggtttcgtca cggaatcctt aatccatggc
    23161 atactttttg caaataattt taacgggagg aacaggccct taacgctcct gtttcaagtc
    23221 ggttttatct tccatctatt caataatcag atgctttatg cgctgcatgg gcattgatgc
    23281 catgaatgcc ctttccgtta ctaccaaaaa caccgattta aaatacggca ccagttatca
    23341 tgagaacttt ctgacctatc tgcagttttt ccaggacaat aatatcgtgg cttcctgtgc
    23401 gcaaaccgac tccaaaggta atcgcagata ccgacccggc gaacagcccg accccgacca
    23461 gtacctgcat gtggtggaaa gaaggccgga tggcgtagtc gtcagaggaa ccaaacttca
    23521 taataccatg tccccttatg tggatgaaat catcgccatt cccacccggg ctttgcttaa
    23581 aaatgtaaac agattatgcc accgcctgtc agatagccgg tgtacatggg ggcggctcac
    23641 ccattatgga agatatcgcg atcatgggtg cctatgacgt ggaaaacaac aaaagaatgg
    23701 cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatggcga gggatcttct
    23821 gaaaatgtgc cgggaaaaac ttagcactgc agaggaggcg gtgaagctcg caaatcaggc
    23881 gactggacta ctatggcatg ttcaacggca aaccggtcgc gctggcaccc aataaaatcg
    23941 aataattcat aatatcgtta aaaaaacctt gataatagtc ttttccgtat aactaagtga
    24001 caaaagccaa agcctggcca gcagtcaacc ttcccggcca gactttggct tacgcactct
    24061 ttcatgcctt acatgttcgc ctgtattggc agtttgcttc ttttttctgt tttgcctcat
    24121 acattgcttt atccgccaat ttcaacaaat cctcaatatt gtcggcatcg tgaggaaaca
    24181 tactaaatcc aatactggca cttatttgaa cagctccgtc ctgaagctca atgctgggcc
    24241 gtaatgcttc ctgaatcttg gcctcaatgg tctcaaacgt ctcaacgttg ccgcggtgcg
    24301 gcaatacgat agcatactcg tctcccccga ttcttgatat gatatccgac tcccgcaagc
    24361 ttcctttcag ccgttcagca acctcgcaca gtacctggtc gcctgccata tgtccccatg
    24421 tatcattaac gattttgaaa tcatccaagt ccagaaacgc caccgtcaag acatcagcaa
    24481 ccattttggc aaaagccagt tccttcgcaa acaagagatg aaaatactgt cgattgggca
    24541 atcccgtcag cgcatcatgg taagcagcat ggcgaatgat tccttcagca acctttcgct
    24601 ccgtgatatc gtagatttgc agtaaccaac cgatgaattc gtttttttcg tttttcacca
    24661 gggtaaagga aacctcgtaa tagttgggga ttttatcgct gccgaaagcg acttcaccaa
    24721 ccgcatcttt tccttgctta aaaatctcaa tcaacgcagg caatttgccc aattcggttt
    24781 ctaaaaaatg accactgaag tctttggcag gcaggtcaag catttttctg gccgcaggat
    24841 tgacatccag aaatctgcct tcacgatcca atataatcat tcccgttctc atttcctcaa
    24901 taatgattga atgggcgatg ggaacaacgt caaacagtcg gtagcggaag atcccccagg
    24961 agattatcaa cgcagctaat ccgatcaccg gcggggtaat gtcaacgtga aaaggattca
    25021 tccccgtgac ttgaaaaaca tttgaaatta cgggcagtaa aagcgcaata aacagcaatg
    25081 acaattgctt gcgatacaag gatatttttt cccggaaacc catggcaaga atggttaaag
    25141 taaagctggc aatgacataa ttgtatacgg cgaaggccca aaagcctggg ccgtaagtct
    25201 tccccaccgt agggaaggcg ccgcttgtat ccaaaaaaac attttgccta accaagccat
    25261 gaaaatcatt gctccaggct aaaatattca ccacgattgg aaccacaagc aaaatcatca
    25321 ccagccagcg tgacttgagc cagcgttcat gcccggtaaa ctggagtgcc aaatataaat
    25381 aggatatcga gatcagggtg atgggaatat attgaagatt ggcccaaaat attttggtcg
    25441 ccaaatcaag cgctgccatt tccaaggctt gaccggcaat ccaaaattca gccaagagca
    25501 aagtcaacag aaaataacgt gcacccagaa cggtgcggtt tttccaggta aaaaacagca
    25561 acgttaccag tatacatgta ataaacaggg atatacaaag atacggcacg cattggtatt
    25621 gtatcgttat cactcacttt acgatttttc aatataccaa ctattttagc ctacagcaat
    25681 tatccaacac aatttgtgtt ttgcatatac tctcagctaa aaattttcct catgccattc
    25741 cctgaaccga aaacgtactg caaacattta acaggcaaca atgtttcacg tgaaacattt
    25801 tccctccctg aaaacatcga ttttttggcc ccaggatact gcaatgcatt ttttcctttt
    25861 ccacctgccg ccctttggaa cttggtaccg tcaatagttt ttcgcaaatt tgatttgcca
    25921 cttggtagat ggcaatt
//
LOCUS       DAOTHZ010000027        25609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2224_length_25609_cov_7.718244, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000027 DAOTHZ010000000
VERSION     DAOTHZ010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 25609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25609
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2224_length_25609_cov_7.718244"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1197
                     /locus_tag="P5549_04870"
     CDS             25..1197
                     /locus_tag="P5549_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW12435.1"
                     /translation="MKIRKKIALLICVMMLGTLLAGCAGGQSGEKTAEGDTIKLGFLG
                     AKTGSVANYGLPGEKGMKMAIEEINAKGGVLGKQLEGIYEDNKGESSEIANIATKYIT
                     QDKVVAMVGDPCTGLTKVAADIAQKSKIVIFSAGATGEGVVEIGDYVFRNTLLDKFAA
                     PAVVDWMINKQGWKNIAVITSMNNGYSTALTPVFKEAIQAKGGKIVLEDSVNDGETDF
                     TAQVTKLKNANADVLVFTGYYTEAALIMNEVQKKGLDIKLVGGDGLYGQDLAKLGQSA
                     VEEKVILYCGFSSDQPSPETEKFITAYQAKYNEDPDMFSAQYYDAVMILAKAMEEAQS
                     TDPSVFKEKLAQLKDYPGVSGKTTIREDREPLKSPVFLLTIKDGKFALEDKIPVNM"
     gene            1415..2290
                     /locus_tag="P5549_04875"
     CDS             1415..2290
                     /locus_tag="P5549_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW12436.1"
                     /translation="MFFEQLINGLTLGSTYALIALGYTMVYGIVQLINFAHGEIYMFG
                     AFAGLFLVAWFDINIFWALLGAMAFCMLLGVLVERIAYRPLRGKSSRLSALISAIGVS
                     IFLSTLMALLRGTNTTRFPQVMSEKTFHLGSVELSSLQVIILLVSALLMIGLQLMIQK
                     TRIGKAMRACSQDIDASSLMGINVNRVISFTFAIGSALAAAGGVMVGIYYNAVWPYMG
                     TMAGLKAFSAAVLGGIGSVPGAMVGGLTLGVLEIMGVAYLSSSYKDAIAFAILILVLI
                     VRPQGLMGQKISKKV"
     gene            2300..3187
                     /locus_tag="P5549_04880"
     CDS             2300..3187
                     /locus_tag="P5549_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW12437.1"
                     /translation="MDAIISQYIDAYYFRILILIGINIILALGLNLTTGVTGQLSMGH
                     AGFMSLGAYTSAILTVHYTVPFFLALLGGACVAALFGFLLGLPTLRLEGDYLAMVTIG
                     FAEIVRVFFLNFEPGGKAVGFSGIPQDTTFTLVWVLVIIVAYFNYKLLHSRTGRAMYA
                     IRENEIAAEASGVNTTQIKVLAFTTGAFLGGLGGGLFAHYMFFIEPGNFGFMKSIELL
                     NMVVLGGMGSIPGTILGTVILTIAPEMLRIVQDYRLLFYGALLVILMIFRPNGLLGDV
                     RLYDIKKRFSRKEVKSWQS"
     gene            3175..3939
                     /locus_tag="P5549_04885"
     CDS             3175..3939
                     /locus_tag="P5549_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12438.1"
                     /translation="MAVLTLEGISQEFGGLRALDSVHLNIEEKEIFGIIGPNGAGKTT
                     LFNIITGIYVPTEGKVVFNGQVLNNLPAYDIARLGIARTFQNIRLFSKLSVLDNVRVG
                     SYGVSHSGFLAGMLGLPSSRREEKKVQVNALQLLELVDLADKRYEYADNLSYGEQRRL
                     EIARALALEPKLLLLDEPAAGMNSTEKEELMRLIRKIREQRQLSIILVEHDMNVVMNI
                     CERIAVLDYGRKIAQGIPEEIKNDDAVIQSYLGAGA"
     gene            3936..4799
                     /locus_tag="P5549_04890"
     CDS             3936..4799
                     /locus_tag="P5549_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRW12439.1"
                     /translation="MKNARFYVPIALSVVCIATSAIIIRYSSAAPMVIGFYRVLFTFL
                     LALLLDGRHIISECRQLNRNDYQLITGAGIFLALHFSFWITSLEHTSVSSSVLFTNLQ
                     VIFVLLFSIFILREKLNGWIAGGILIALAGSFCIGTGDLARGKIWGDLLALASGLFVA
                     IYFIIGRKVRIRVGIWTYTAVVSLVAAVILLAASLVMDLNLSHFPPRQWLLFFLLALG
                     PGIAGHGILNWALKYVKAPIVSVSILGEAVGASIFAWILFAEALSWYQIAGGLLILSG
                     IYLAASHEEPA"
     gene            complement(4821..5765)
                     /locus_tag="P5549_04895"
     CDS             complement(4821..5765)
                     /locus_tag="P5549_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRW12440.1"
                     /translation="MLLATLLIFTGCQQQAAKKPAQPIHKKPVTLAPEVSKIKQEPTI
                     TLYRSATGQKQELKMEQYIKGVVAAEIGPKFPMEALKAQAIVARTMTLALIHYENGTQ
                     GKHGTDASDNHTEFQAYDEKKITDRISQAVDATRGQILTYNGKFVYGEFHSVSKNKTA
                     SIAESFPKLKEKAGAYIVPVTTNGIKYAPAKYRNWTVRVPRSEIMQIMGPKAGNLNDI
                     RISNKGPSGRALTISAGSASIAGVDLRQQVGFDRLFSTCFTSIKAQGNGIVFKGSGWG
                     HGAGMEQWGAYAMAKEGKKARQIVEHYYPGTTWNRLYK"
     gene            5970..6428
                     /gene="trmL"
                     /locus_tag="P5549_04900"
     CDS             5970..6428
                     /gene="trmL"
                     /locus_tag="P5549_04900"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRW12441.1"
                     /translation="MEIVLVSPEIPQNTGNIARTCALTGTRLHLVKPLGFSLADRYMK
                     RAGLDYWEHVKLQVWNDFEEFAQTYAQRKLYLASTKADFSYDQIRYHSEDVFVFGCET
                     RGLSPAILERYPQQLIRIPMIDLGRSLNLSNAVAVILYEAMRQQGFKGLC"
     gene            6445..9135
                     /locus_tag="P5549_04905"
     CDS             6445..9135
                     /locus_tag="P5549_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW12442.1"
                     /translation="MKPEKLLYSMFLALGIIMLVLTGIYLYSLVSFSIGDAQAGWLYF
                     AITGLICSLAMTYVWLNKHIRSFLPGRRAQSAEKKPAPFRPISQERGYPEDDQAFHFS
                     LSAEEQLNYLNKLIDNINEFVCIFDRAGHIRYANRKAWQTWRYSRQEMEGKRIDDFFP
                     EVYHRNLRMEMDHILATGNYRIFEIPVLYQDGSEHPVRLHLAQINEPGESEALMALAE
                     DITVYRQTQEILRQANDEMENRVKERTIELEKTNEILRTQIKERKKIEEALRISEEKF
                     AVAFRLSPEPININAMSDGRYIDVNDAWLAYSGYTREEVIGRTPEELNMQMEPELSKE
                     QFKLLMTKGWLKDLETKYTNKRGEVRLSLCSMAAVTINHELCALMASSDITERRSIEK
                     DLASQTERLMVTLRNIADGVVATDINGKIIVVNEAAEIMMGRTAEEAVGSDFFQTVLD
                     LNPNPWPVYEDLMSSLPTAKKNYNMTFSLHGEERSVEAKRAIINDREGNFIGMVWALR
                     DTTDEQRIKDELMKASKLDSLGVLAGGIAHDFNNLLTVIAGNLALIKMMHESDEEMIA
                     LLGEAEKASFQAKGLTQQLLTFARGGAPVKVPVDIDQLLTGVVNFALSGSSVRSEVYK
                     PDRLWPVMADLGQLNQVANNLLINAVQAMPKGGTIRITGENLVIGAEEIATLPAGRYV
                     CLSVADEGVGISETILSRIFDPYFTTKPTGSGLGLATSYSIIRKHHGCITVDSQPGRG
                     TIFKVYLPACDKEKISSTDDPFILAQGRGRILVMDDDERICEVASQILDSIGYEVQTA
                     ADGSAALQAYQEAMEKGKVYDVVILDLTVPGQMGGKQTIQELLKIDPLACVIVSSGYS
                     NDPIMADHARWGFKGVIPKPYGFKELGETVANVLKQKQIH"
     gene            complement(9214..9390)
                     /locus_tag="P5549_04910"
     CDS             complement(9214..9390)
                     /locus_tag="P5549_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW12443.1"
                     /translation="MAYVITDECISCGVCVDECPVEAISEGEDKYVINPDLCTDCGSC
                     ADVCPVEAPVPADE"
     gene            9620..10258
                     /locus_tag="P5549_04915"
     CDS             9620..10258
                     /locus_tag="P5549_04915"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRW12444.1"
                     /translation="MRCIIMANGQYGANAFYKRKFPEGDRIICADGGANYAYQLGWYP
                     SAIVGDMDSILPEVREYFTSLGVPFTLLSCRKDETDTQAVLAMAQAMGATEIIMLGTL
                     GGRLDHTLSNLYSGLDAIKQGIKITHISPAVRVDLVNRELAVKGQKGDLVSILALTDE
                     TQGVTIEGFEYPLQNVCLRKSDPYAVSNVMTGTNGTVFVREGILAVFHYGQS"
     gene            10284..11111
                     /gene="cdaA"
                     /locus_tag="P5549_04920"
     CDS             10284..11111
                     /gene="cdaA"
                     /locus_tag="P5549_04920"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRW12445.1"
                     /translation="MDPSLLSGVSASPWNIFRNLLDILIVAYIIYRLLGLIRETRAEK
                     LLKGLIILLLFSVVVSILDLHMLNWLLDKLWIIFAITLPIVFQPELRRFLEQLGQGSF
                     FKSSHGNQDAQSYQAIIAAITEATSILSRDKTGALMILARQTGIAEYTESGISLDALV
                     TAGLLVNIFVPNTPLHDGAVVIKEGRLSKATCVLPLSENTELPGDLGTRHRAGLGITE
                     VSDAIAVIVSEETGKISMAIDGRLYRPLQPQYLQEMLTRELLAAPEKQGLWRRWLSR"
     gene            11108..12037
                     /locus_tag="P5549_04925"
     CDS             11108..12037
                     /locus_tag="P5549_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRW12446.1"
                     /translation="MNNPDERHKKSGIKVLSLLMAVMLWFYVVNQGGAATGQNYQQTK
                     LNYYNLPAGLTVNGPEAVSVRIWGSARDNGNVIAYVDLTGLQPGVYHLAVKVKPLQGV
                     MFATVQPDKVSIELKSQADRSFTVQYEVDRNLPEGKELHDVTLSPGQCIVKGEQDAVS
                     KVASVHARINVGDTTGLISQQAVLVARDAQGKEISSGLQLIPTVVNVYAVADSKKISK
                     ELKVKLKFKGDPAAGYQLGTAVADPATVTVLGEETRLNNMEEISSQEIDLADHRVSFT
                     QMVALPALEGMIIFPTQIKVMVNIEPVDSEVEP"
     gene            12034..13716
                     /locus_tag="P5549_04930"
     CDS             12034..13716
                     /locus_tag="P5549_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRW12447.1"
                     /translation="MKVRVREIRLSLEEDESRLPLLIASRLEINVRHIKHWKLVRKAV
                     DARRRSIYFSYTIDIELAEGITIASQLLTQPGISLLEDQPTAQLMSGSNRLAVAPLIV
                     GAGPAGLFAALLLARHGYRPIVIEQGQDVDQRVQAVERFWSGGVLDRCSNTQFGEGGA
                     GTFSDGKLTTRIGDQRVDYVLRTFAEFGADPEIMYVKKPHVGTDRIRQVVKNIRREVI
                     RLGGEFYFSARMTDILVEQKQLKSIIINNEAEISCEVLVLAVGNSAREVYHLLHRKGV
                     SLIPKAFALGVRVEHPQHLIDRIQYGQYAGHPRLPVADYQLTYQDRPTGRSLYTFCMC
                     PGGRVIASSSAPGEVVVNGMSYFSRDSGLANSAMVVTVHPEDWENDPLGGVHLQQQLE
                     QKAYAMGGENYHAPAQYLHDFMAYQASGNLNNSMASYRPGVTGVNLWQLLPQEIAEVM
                     RRGLLHWDKKAPGFISEEAVMTGVETRTSAPLRIERDQTMNAVSMEGIYPCGEGAGYA
                     GGIVSAAVDGLKVAEKIISQYERPNFMPLINNHQVQRGSQLPNEERNTLPNT"
     gene            13809..15161
                     /gene="glmM"
                     /locus_tag="P5549_04935"
     CDS             13809..15161
                     /gene="glmM"
                     /locus_tag="P5549_04935"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRW12448.1"
                     /translation="MGILFGTDGVRGIANLELTPEMAFDLGRAGSFILAQQDSNSRPR
                     IMVGKDTRVSGDMLEAALLAGICSTGADALTLGVIPTPAVAYLTQQYNASCGVVISAS
                     HNPVQDNGIKFFGPNGYKLPDETEAQIEKLVLQGTRELVRPQGADVGRIYDVAEATNR
                     YLAFLGSCTQEKDFSNLTVVVDCAHGASYQAAPRLWESLGVKVIAINDQPNGININDR
                     CGSTYTASLKKTVIHHKADLGIAYDGDADRCIAVDERGNELDGDFIMTICALDMFRRG
                     ALQPNRIVATVMSNIGLDIALKREGILVDSCAVGDRYVLERMMETGALLGGEQSGHII
                     FLEHATTGDGLLSSLKLAEVMKRTGLPLSELAREMEKQPQMLVNIKLEDKDTILNHAL
                     VLEAMQTAERKLGEQGKLVVRPSGTEPLVRIMAQGPYQDLLQEVIDEIRASIEKAQQK
                     "
     gene            15361..17181
                     /gene="glmS"
                     /locus_tag="P5549_04940"
     CDS             15361..17181
                     /gene="glmS"
                     /locus_tag="P5549_04940"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRW12449.1"
                     /translation="MCGIMGYIGSGEAVPVIIDGLSKLEYRGYDSAGISFYQNGRLQL
                     AKKKGRLAELVKCLGEFPSAHLGIGHTRWATHGVPSDRNAHPHMDCSGRIALVHNGII
                     ENYASLRKQLMLEGHDFVSETDTEIVAHLIEKFYRDCSLEAAVREALQHLKGSFAVAV
                     ISSDEPDKIIAAKKDSPLVVGAGNGENYLASDIPAILGKTNQVYIIEDNQFVILTKDA
                     VSITDFSGQPVEKKLFPVTWDPIAAEKGGYEHFMLKEIHEQPTALRETLRGNVKEQII
                     DLGYLDIDPMFKDVEKIYIVACGTAYHAGLVGRQALEKLARIPVETDIASEFRYRDIL
                     WNPRSVMIVISQSGETADTLAVLREAKRQGIKVLAVTNVVGSAVSREADRVIYTHAGP
                     EIAVASTKAYSTQLLLMYLLALHLANLRSTCSRETLQELTADLEIMDQKVEAVFSQEE
                     KIRQLAEKYYQVESTFFLGRGFDYAVAMEGALKLKEISYIHAEAYAAGELKHGPLALI
                     EEGVPVLALITQDQLVEKTMSNIKEVKARGAIVVAICKENLQEACQECDEQIILPDVN
                     PILAPIVGVIPLQIFSYYMAILRGADVDKPKNLAKSVTVE"
     gene            17321..19129
                     /locus_tag="P5549_04945"
     CDS             17321..19129
                     /locus_tag="P5549_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12450.1"
                     /translation="MTNKSGFDLVLENIDFRKFADTLSVIIFAYADNHKYIYVNPAFE
                     TALGYSLEELLQMNFWDVCHPDYKDLVRERGQARLRREAVPANYEIRALRKDGSDLWV
                     DVFFSVTQVAGKLISIVGAYDITERKRLQEALVMSRDRLEMCVQERTLELSQKNLELT
                     LLNRNLNTVIMNMSDGVVFLHQSGDFELLNPDFHKSWQESHRGFEENFREWCSDPDNK
                     YMKRLFVSGKAFQEEEIVIPMAGTSLRFLASGTPILSEQGMVNTAVLIIRPLKEVHHM
                     VSRFSGYTARFHFKDIITCNPQMLEIIEVARMAARNTSNVLIEGESGTGKELFAQAIH
                     NASQRSAGAFVALNCGAIPRELIASELFGYNEGAFTGAKKGGNPGKFELADGGTLFLD
                     EIGDMPLELQVALLRAIQEKSISRIGSNQIIPVDVRIICATNKNLSEEMQKGNFRQDL
                     FYRLNVIDLHILPLRERPEDILLLFHHFLAQLSSATHSVLEEELKNSLLSYQWPGNVR
                     ELQNVAERIVNLDHLRDMPLNLSWQHAGLLEEKKAGHHFSIQQAQTKYKNERVEQERH
                     RVISLLKQYDGNVSRVAREMGISRSTLYRKMKNLSF"
     gene            19429..21054
                     /locus_tag="P5549_04950"
     CDS             19429..21054
                     /locus_tag="P5549_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW12451.1"
                     /translation="MTERRWQQWYDPGVPTTCYYPLTTMKDEFIKWVRANPDKPYIYC
                     NDLTYSYQETNHIAKKLANVLLELGVKKGDRVSLVLPNIPEFIFSAHAVMKTGAIIVP
                     VNPLYTVPEMVYNFSDSGSETVIVYGPVAPRILEVMRSGQTQLKKMIVMQVPGKEVAL
                     EAAPDILDYASVIEAASDLEPEIHLDNKDTVILIYTGGTTGVPKGCCLTNSNFVAFYT
                     VFIEWARAMFPPQELRTLCTVPLYHIYGFNMQINANLASGGTMILVPEVTPDNVLDAI
                     DRFLPTYWPAVPALIQMLNMHPRLPQSKASSIRFLLSGSAPLPVEAMKKFEELTGSTI
                     IEGFGGSETTNGVMVNPKGKRKPGSVGIPFPDMDVRIVDLKNGTEEVPIGEPGELHLK
                     GPLIIEKYWNKPEETANAIKDGWFNTGDIATMDEDGYITIVGRTKDMIISSGFNVYPR
                     DIDELLYTHPKVLDAGAIGVPDPIRGESVKVFVVLKPGETMTEEEVIEFCRQSLAAYK
                     VPRYVEFLEDIPRTNMKKVDRKALREMEATRQK"
     gene            21304..21723
                     /locus_tag="P5549_04955"
     CDS             21304..21723
                     /locus_tag="P5549_04955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRW12452.1"
                     /translation="MNIDAKIAELAEKNKDVPFYNFIGLEYGKAGENYAELMLEVKKE
                     TMNVHGVLLGVMYYALCDVSTSVAVEHSLPEGYYYVTNDLNVSIFSSVSSGQVRARAE
                     VLKTGKRLAFVESRIYNDRDELLAVGRVTKTRLAKHQ"
     gene            21769..22734
                     /locus_tag="P5549_04960"
     CDS             21769..22734
                     /locus_tag="P5549_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRW12453.1"
                     /translation="MRNPMFDVLKGLGIISVVFSHVYRGGTDPLAVFIREVAMWSVPM
                     FFIVQGHFMQSGLQKGWLTSSWDKIKKSYVPYLYWAVAYGAFYYFTIGKTFSAMDLIL
                     GKTALHLYYMFYYIIFAVFMPLLYFLPKLWRVAILWLMILVNIYWVLMLELSRLYHFH
                     WISYSGPVPMKWWGFIAIGMLVAEYPQIKEYICQHARAFLIGGLAVAAVGTIIPYINH
                     TTGYLFNKVEIFPLSIGLVLALAIYYSRDDAFGKQTLAYIGERTFGIYLAHFFMVDYL
                     RQTLLPGDRTLVVIIILVICILVKDNKDRIKAAWKEKRSGKQNVA"
     gene            22768..23313
                     /locus_tag="P5549_04965"
     CDS             22768..23313
                     /locus_tag="P5549_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HRW12454.1"
                     /translation="MWAKISRFYPPWLDVICVICLGFIWLYTAVRYGLMADQIPTHFG
                     FSGTPDAWSSKSFGSVFMLLLIGTIVWMSMFLINYFLIIKPEDPRKYINLPQGRKDAM
                     SLEEVEALRRITVQGMAIINLTVVLMFVFIQYGMVNSALGLQNGLGWGVNVLVGILLI
                     ETAWLTWKTFSLSKNASGARR"
     gene            complement(23443..23745)
                     /locus_tag="P5549_04970"
     CDS             complement(23443..23745)
                     /locus_tag="P5549_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRW12455.1"
                     /translation="MEITKTQKNNTVTIALGGRLDTVTHTELASELEKIFAADKANLV
                     FDLSSLKYISSAGLRVFLTAQKKVNAQGTTMKITGMIPAVKEIFELTGFSTIMTIE"
     gene            24010..>25609
                     /locus_tag="P5549_04975"
     CDS             24010..>25609
                     /locus_tag="P5549_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRW12456.1"
                     /translation="MIFLKINDVIKQISINNRNLADIRWESDANALAKARAFAWMLEK
                     DPDLVNHYEELEKIRQLIDVDELHITDGEGILISGTVKEYYLYRFDADKQSAAFLPAI
                     HDKTFELAQDPQPKGINGEMFQYVGVARKDADGIVQIGYRPEKLANAMEVADIKNLAP
                     GFRVGNSGSVIIATKTGVIVSAADKNFIGKTVYEFCHGYDPKGTSGSFIRDAYDGRSL
                     FAFKVTGDYMILGKLPADEMYLSRNSTTTVMILFNILLFSLIFFLVARLVQTVVIDGI
                     FAVNKSLVQITQGNLDEKVEVNTSEEFQALSSGINGMVEALKDAIKEAAARIDDELAF
                     ARAIQLSALPNHFPAFPDKSEIDIFADMHPAKEVGGDFYDFFFVGENQLAVVIADVSG
                     KGIPAAMFMMITKTLIKNLALTGISPAEVFINANQSLCENNDAGMFVTAFMGLLDCET
                     GILRYVNAGHNPPLIKKTDGTAAWLQVKPGLVLAGMEGVTYIEEEIRLQPGDMLFLYT
                     DGVTEACNHALELYTDHRLLEELNH"
BASE COUNT         6620 a         5641 c         6769 g         6579 t
ORIGIN      
        1 aatactattg aggagggtac aggtttgaag attagaaaaa aaatagcgct gttgatttgt
       61 gtgatgatgt tgggcacctt gctggccggc tgtgcaggag gccagagtgg tgagaaaacc
      121 gccgaaggag acaccatcaa attgggattc ctgggagcca agaccggatc agttgccaat
      181 tatggtttgc ctggtgagaa gggcatgaaa atggccattg aagaaattaa cgccaaggga
      241 ggcgtactgg gaaaacaact ggaaggcatc tatgaagaca acaaaggcga gagcagtgag
      301 attgccaata tagccaccaa atacattacc caggacaagg ttgtggccat ggttggtgac
      361 ccctgtactg gtctgaccaa agttgctgcg gatattgccc aaaagagcaa aatagtcatt
      421 ttctccgccg gtgctaccgg tgaaggggtc gttgagatcg gcgattatgt cttcagaaat
      481 acgctgctgg ataagttcgc cgcgcctgcc gtagttgatt ggatgatcaa caaacaaggt
      541 tggaagaaca tcgctgtcat cacttccatg aacaatggtt acagtacagc gctcactcca
      601 gtattcaaag aagccatcca agccaaaggc ggaaaaatag tgttggaaga cagcgtgaat
      661 gacggcgaga cagattttac cgctcaggtg accaagctga agaatgccaa cgctgatgtg
      721 ctggtattta ccggttacta taccgaagct gccttgatta tgaatgaagt acagaaaaaa
      781 ggtctggaca ttaaactggt tggcggagac ggtctgtatg gacaggatct ggccaaattg
      841 ggacaatcgg cagtggaaga aaaggtcatt ttgtattgcg gattttccag cgatcagccc
      901 agtcccgaaa cagaaaaatt cattaccgct tatcaggcca aatacaatga agatccggat
      961 atgttctcgg cacagtatta cgatgccgtc atgatcctgg ccaaagccat ggaggaagcc
     1021 cagagcaccg atccaagtgt tttcaaggag aaactggccc agttgaagga ctatcccgga
     1081 gtatccggca agaccaccat tcgtgaagat cgtgaaccat taaagagccc ggttttcctg
     1141 ttgaccatca aagacggcaa atttgccctg gaagacaaga tcccggtcaa tatgtaattt
     1201 catgctctga catatttctt caggtgctta aaagccggac cgcaagttcc ctggaacttg
     1261 cggtccggct tcttgatctc attgttatct gcaatgatca gacagttttt tacgtttatc
     1321 gtttgcgcag tcgcaataga tgatataata tttatgtttg gatgccgaaa tgaatacgct
     1381 tgcagattga ataagacttg gcgggaggat acgtatgttc tttgaacagt tgatcaacgg
     1441 tctgaccctg ggcagcactt atgcactgat cgcattgggt tatacgatgg tatacggaat
     1501 cgtacagctg atcaatttcg cccatggaga aatatatatg tttggagcct ttgcaggatt
     1561 attcctggtg gcctggtttg atatcaatat cttctgggcg ttgctgggag ccatggcttt
     1621 ttgcatgtta ctgggcgtat tggtggaacg cattgcctat cgccctttac ggggcaagtc
     1681 ttcccggctg tcagcgctca tcagtgccat cggtgtttcg atttttcttt ccacactgat
     1741 ggctttatta cggggaacca acaccacccg ctttccacag gtcatgagtg aaaaaacatt
     1801 tcatttgggt tccgtggaac tctcctccct gcaagtaatc attctgctgg tttccgccct
     1861 gctcatgata ggtttgcagt tgatgattca aaaaaccaga atcggcaagg ccatgcgggc
     1921 ttgttcccag gacattgatg cttcttccct gatggggatt aatgtgaatc gggtcatctc
     1981 ctttaccttt gccatcggtt cagccttggc ggctgccggc ggtgtcatgg tggggattta
     2041 ctataatgcg gtctggccgt acatgggcac catggcaggg ttgaaagcct tttctgccgc
     2101 agtgctgggc gggatcggat cggtgccggg tgccatggtt ggcgggttaa cgctgggcgt
     2161 gttagaaatc atgggggttg cgtatctttc ttcttcttat aaggacgcca tcgcctttgc
     2221 catcctgatt ctggttttaa tcgttcgtcc ccagggtttg atgggtcaaa agatatcgaa
     2281 aaaggtgtag gtgagctgaa tggatgcaat catttcacaa tacatagacg cctattattt
     2341 ccggattctg attttgattg ggatcaatat tattctagcc ctgggactga acctgaccac
     2401 cggcgttacc ggtcaattgt cgatgggaca tgccgggttt atgagtctgg gagcatatac
     2461 ttcagccatt ttgaccgtgc attataccgt gccgtttttc ctcgccttac tgggcggggc
     2521 atgtgtggca gctctgtttg gctttttact ggggttaccg acgctgcgcc tggaaggtga
     2581 ttacctggcc atggtgacca tcggatttgc ggaaatcgtg cgggtgtttt tcctaaattt
     2641 tgaaccgggt ggaaaagcag tcggtttttc cgggattccc caagatacga cgtttacctt
     2701 ggtatgggtt ctggtcatca ttgtggctta tttcaattat aaactgctcc attcccgtac
     2761 cggccgtgcc atgtatgcca tccgtgaaaa tgagatcgcc gctgaagcca gcggtgtcaa
     2821 taccacccag attaaggtcc tggcctttac caccggagca tttctggggg ggctgggggg
     2881 aggattgttt gcccactaca tgttctttat tgaaccgggc aactttgggt ttatgaaatc
     2941 aattgaattg ctgaatatgg tggtgctggg cggcatgggc agcatcccgg gcaccattct
     3001 gggcaccgta atattgacta ttgccccgga aatgctgcgg atcgtacagg attaccgtct
     3061 cttgttctat ggcgcgctgc tggtcattct aatgatcttc cggcccaatg gattgctggg
     3121 cgacgtgcgc ttgtatgata ttaaaaaacg cttttcccgt aaagaggtga aatcatggca
     3181 gtcctaaccc tagagggcat cagtcaggag ttcggtggtt tacgggcgct tgattccgtc
     3241 catctgaaca tagaagaaaa agaaatattc gggatcattg gccccaacgg tgccggcaag
     3301 accaccttgt ttaatatcat caccggcatt tatgttccta cggaagggaa ggttgtcttt
     3361 aacggacagg ttttgaacaa tctgccggct tatgatattg cccggttggg gattgcccgt
     3421 acatttcaga acattcggct gttcagcaaa ctgtcggtgc tggacaatgt acgggtaggc
     3481 agttatggtg tcagtcacag tgggttcctg gccgggatgt tgggattgcc ttcttcccgt
     3541 cgagaggaaa agaaggtcca agtcaacgct ttgcagctgt tggaactcgt agatctggct
     3601 gataaacgtt acgaatatgc cgacaacttg tcctatggtg aacagcgccg cctggaaatt
     3661 gccagggctc tggccctgga gcccaagctg ctgttgttgg acgagccggc agcgggaatg
     3721 aattccaccg agaaagagga gctgatgcga ctcatcagaa agatcaggga acagcgtcag
     3781 ctgagcatca ttctggtgga acatgacatg aacgtggtga tgaacatctg cgaacgtatc
     3841 gctgtgcttg attatggacg caaaattgcc cagggaatcc cggaagaaat taaaaatgat
     3901 gacgcggtca ttcaatccta tctgggggca ggcgcgtgaa aaacgcacgc ttctatgtcc
     3961 ccatcgcctt atcggttgtc tgtatcgcca cttcagccat cattattcgc tattcttctg
     4021 cagctcctat ggtcatcggt ttttaccggg tgctttttac tttcctgctg gccttgctgc
     4081 tggacggacg ccacatcatc agcgaatgcc ggcagctaaa cagaaatgat tatcaattga
     4141 tcacgggggc tggaatattt ctggcgctgc atttttcttt ctggatcact tcgcttgaac
     4201 acaccagcgt atcaagttcg gtattgttta ccaacctgca ggtcattttc gtattactct
     4261 tttcaatatt tatcctgcgg gaaaaactga atgggtggat tgccgggggt atattgatcg
     4321 cactggcggg gagcttttgt atcggaaccg gggacctggc ccgtggaaaa atatggggtg
     4381 atctgctggc cctggccagc gggctgtttg tggcaattta ttttatcatc ggtcgcaagg
     4441 tccgaatccg ggtgggaatc tggacttaca cagccgtcgt ttcgctggtg gccgcggtca
     4501 ttctattggc tgcttcgctg gtgatggatt tgaatctaag ccatttcccg ccccgccaat
     4561 ggctcttgtt tttcctgctg gcgctgggac cggggatcgc cggacacggg atattgaact
     4621 gggcgttaaa atatgtaaag gctcccatcg tctctgtctc cattctcgga gaagccgtgg
     4681 gagcaagtat ttttgcctgg atactgtttg cagaggcgct gtcctggtat cagatcgccg
     4741 gcggattgtt aatcttgtcc ggcatttacc tggctgcgtc tcatgaagag ccggcataga
     4801 gcatcaaatc ccggcaagtc ttacttataa agcctgttcc aggtcgtacc cgggtaataa
     4861 tgctctacga tctggcgggc tttttttccc tccttggcca tggcgtaagc tccccattgt
     4921 tccatcccgg ccccgtgacc ccacccgctg cctttaaaaa caataccatt gccctgcgcc
     4981 ttgatggagg tgaagcaggt cgaaaagagg cggtcaaaac ccacctgctg acgcagatcc
     5041 accccggcga tggaagcaga accggcggat atcgtcaaag cacgaccgga aggcccttta
     5101 ttactgatcc ggatgtcatt caaattgcca gctttgggtc ccattatctg cataatctcg
     5161 ctgcgcggca cacgcacggt ccaattgcgg tatttggctg gggcgtattt gatgccgttg
     5221 gttgtgaccg gtacgatata tgcgccagct ttttctttta gcttgggaaa actctctgcg
     5281 atactggcgg tcttgttctt ggatacagaa tgaaactcac cataaacgaa tttcccgtta
     5341 taggtcaaaa tttgaccgcg ggtggcatcg accgcctggg agatgcgatc ggtgattttc
     5401 ttttcgtcat aagcctgaaa ctcggtatgg ttatcactgg catccgtgcc atgcttgccc
     5461 tgggttccgt tttcataatg gatgagtgcc agggtcatgg tacgggcgac gatggcttgg
     5521 gctttcaggg cttccattgg gaattttggc ccgatctcgg cagcgactac gcctttaata
     5581 tattgttcca ttttcaattc ctgcttttga cctgtggcac tgcgatataa agttatcgta
     5641 ggttcctgct tgattttact gacttcaggg gcgagggtga ccggcttttt atgaatgggt
     5701 tgagctggtt ttttggcggc ctgctgttgg catccggtga agatcagcaa ggtcgcaagg
     5761 agcattaagc tgagaaaaag caaccgccat aaccgattta ttttatgcaa attcatactc
     5821 tcctttgccg attattagcc ttagtattgc ctgcagataa aattgttatg agtgacaaac
     5881 ttaggtataa ttcttagtaa tatcaatact ttattctttg gtattcagca tttttcaatt
     5941 gtgcagcgac aatggcagga agtggatcta tggaaatcgt attggtcagc ccggaaatac
     6001 cacagaatac cggtaatatc gcccggactt gtgccttgac cggcactcgt ttgcatcttg
     6061 tcaagccgtt gggattttca ctggcggatc gctatatgaa aagggcgggg ctggattatt
     6121 gggaacatgt aaaactgcaa gtatggaatg attttgagga atttgcccag acctatgcac
     6181 agcgaaaact gtatctggcc agcaccaagg cagatttcag ttatgatcaa attagatacc
     6241 attcagagga tgtgtttgtc tttggctgcg agacccgagg cctcagtcca gcgattctgg
     6301 aacgttatcc gcagcaatta attcgcatcc ccatgatcga cctggggcgt tccttaaacc
     6361 tttccaatgc agtcgccgtc attctctatg aagcgatgcg ccagcaagga tttaagggct
     6421 tgtgttaatc aatggaggga tccaatgaag ccggaaaaac tgctttattc aatgtttctt
     6481 gctctgggaa tcataatgct tgttttaacc gggatttacc tgtattcgct ggtctccttt
     6541 tcaatagggg atgcacaggc aggctggctt tattttgcca ttaccggatt gatttgctcg
     6601 ctggccatga cttatgtctg gttgaataaa catatccggt cgttcctgcc tggacgccgg
     6661 gcccagtcgg cagaaaaaaa gccagcacct tttcggccga tttcccaaga gcgaggttat
     6721 ccggaagatg atcaggcttt tcatttctcc ttgagtgcag aagaacagtt aaactatctg
     6781 aacaagctga tcgacaacat taatgaattc gtttgcatct ttgaccgtgc gggacacata
     6841 cgttacgcca atcgcaaggc atggcaaacc tggcgttaca gtcgacagga gatggaaggc
     6901 aaacgaattg atgatttttt tccggaggtc tatcaccgca atttacggat ggagatggac
     6961 catattttag ctaccgggaa ctacagaatt tttgagatcc cggtgctcta tcaggatggc
     7021 agcgaacatc cggtacgttt gcatctggct cagattaatg agccagggga gagtgaagcg
     7081 ctcatggctt tggcagaaga tattacggtc tatcgtcaga cccaggaaat tctgcgccag
     7141 gcgaatgatg aaatggaaaa tcgggtcaag gagaggacca tcgaactgga aaaaaccaat
     7201 gaaatcctgc gcacccagat caaagaacgt aaaaaaattg aggaagccct gcgtatctcc
     7261 gaagagaaat ttgcagtcgc gtttcgtctc agtccggagc cgatcaatat caatgccatg
     7321 agtgacggac gctacattga tgtcaatgat gcctggctgg cttattcagg ctacacccgg
     7381 gaagaggtca ttggtcgtac acctgaggaa ttaaatatgc agatggaacc ggaattgagc
     7441 aaagagcagt ttaaattatt gatgaccaaa ggctggctca aggatctgga gaccaaatat
     7501 accaataaac gaggcgaggt taggctaagt ctttgttcca tggctgcagt caccattaac
     7561 catgaattgt gtgccttgat ggcttccagt gatatcaccg agagacggtc aattgaaaaa
     7621 gatttggcca gtcaaacgga acgcctgatg gttaccctca ggaatattgc cgatggagtg
     7681 gtggccaccg atatcaacgg aaaaattatc gtggtcaatg aagcagcgga aatcatgatg
     7741 ggcagaactg cggaagaagc agtcggcagt gatttctttc agacagtgct ggacctgaat
     7801 cccaatcctt ggccggtgta tgaggatctt atgtcgagcc tgccaacggc caagaaaaac
     7861 tacaatatga ctttttctct gcacggggaa gaacgaagcg tagaagccaa acgggccatc
     7921 atcaatgacc gggaagggaa tttcatcggg atggtttggg cacttcggga tactacggat
     7981 gaacaaagga tcaaggacga actgatgaag gccagcaaac ttgactcgct gggtgtattg
     8041 gctgggggca ttgcccatga ttttaacaat ttactgacag tcatagccgg caatttggcc
     8101 ttgatcaaaa tgatgcatga aagtgatgaa gaaatgattg cgttactggg cgaggcagaa
     8161 aaagcttctt tccaggccaa aggattgacc cagcagctat tgacctttgc tcgcggcgga
     8221 gcgcctgtga aggttccggt cgatattgat caactgctga ccggggtggt caattttgcc
     8281 ttgagtggct ccagtgtacg cagtgaagtt tataaacccg atcggctttg gccggttatg
     8341 gctgatctgg gacaattgaa tcaggtggcc aacaatttgc ttatcaatgc agtccaggcc
     8401 atgcccaagg gggggacgat cagaatcacc ggagaaaacc tggtgattgg ggctgaagaa
     8461 attgcaaccc ttccggcagg cagatatgtg tgcttgagtg ttgctgatga aggggttggg
     8521 atatcggaaa caattctttc ccggatcttt gacccctatt ttaccaccaa gcctacaggc
     8581 agcgggttgg gattggcaac ttcctattcc attattcgca aacatcatgg ctgcattacc
     8641 gttgattcgc aaccaggccg aggaaccatt ttcaaggtgt acttgcctgc ctgtgacaag
     8701 gaaaaaattt cgtcaactga cgatccattt atcctggctc aaggccgggg caggatcctg
     8761 gtgatggatg atgatgagcg gatctgcgag gtggcatcac aaattctgga ttccattggc
     8821 tacgaagtac aaacggctgc agatggcagt gcggcgctgc aggcatatca agaagccatg
     8881 gaaaagggaa aagtttatga tgtcgttatc cttgacttga ccgttccggg acagatggga
     8941 ggcaagcaaa ccatccagga gttgctgaaa atcgatccgc tggcatgtgt catcgtcagc
     9001 agcggttatt ccaacgatcc tatcatggct gaccatgccc gatggggatt taaaggagtc
     9061 attcccaaac catacgggtt caaggaactg ggtgaaaccg tagccaatgt tttaaagcag
     9121 aaacagatcc attgacgtaa catgggcaaa aaataaaaaa accctgaagg ggttttttta
     9181 tttgaatata gattgatgat ataaggtcaa cttctattca tcagccggca caggtgcttc
     9241 gaccgggcaa acatctgcac aggaaccaca atcagtacag agatcggggt tgattacata
     9301 tttgtcttcg ccttctgaga ttgcttcgac aggacattca tccacacaca cgccgcagct
     9361 gatacattcg tctgtaatca cgtaagccat ctggttcgcc tccttgctac aatcaattat
     9421 ttagaattat agtgaacggt attttttttg tcaatttttt atatccctaa tccagcgcgc
     9481 agacgcggag gaaacagttt tcttaatcat catgcaaaat caattattca tagggtgtac
     9541 atgggaagca aggaatatta caatttgaat gaacagaaac agtagggaaa aacgaaaatc
     9601 aacatcaagg aagataacga tgagatgtat tatcatggcc aatggccaat atggcgcgaa
     9661 tgctttttat aaacgtaagt ttcctgaggg agatcgtata atatgtgcag atggaggtgc
     9721 aaattatgcc tatcaactag gctggtatcc ttcggctatt gtaggggaca tggattccat
     9781 cctgcctgaa gtcagggagt attttaccag cttgggggta ccttttaccc tgctttcctg
     9841 tcgcaaggat gagacagata cccaggctgt ccttgccatg gctcaagcca tgggagcaac
     9901 cgagatcatc atgttgggga cgctgggcgg gagactcgac cataccttgt ccaatctata
     9961 cagtgggctg gatgcgatca aacaagggat aaaaattact cacatttcac cggcagtcag
    10021 ggttgatctc gtcaaccggg aacttgcggt gaaagggcaa aaaggggatt tggtttcgat
    10081 tctggcgctg acggatgaga cgcaaggggt gaccatcgag ggctttgaat acccgctgca
    10141 gaacgtttgc ctgcgcaaaa gcgatcccta tgcagtatcg aatgtcatga ccggaaccaa
    10201 cgggacagtt tttgtgcggg aaggaatttt ggcagttttt cactatgggc agtcatgata
    10261 taaattaaaa tggaggaaag acattggatc caagtttatt atcaggggta tcggcgagcc
    10321 cctggaacat attccgaaat cttctggaca tacttatcgt ggcctacatc atttatcgcc
    10381 tgttgggctt gattcgagaa acccgggcag aaaagctgct gaaagggtta attattttgt
    10441 tgctcttttc agtagtggta agcatccttg acctacatat gctcaactgg ctgctggata
    10501 aactatggat tatctttgcc attaccctgc cgattgtctt ccagccggaa ctgagaaggt
    10561 ttctggaaca actgggtcag ggcagcttct ttaaatcctc ccacggcaat caggatgcgc
    10621 agagctatca ggcgattatt gcagccatta cggaagcaac ttccatccta tccagagata
    10681 aaaccggggc tttgatgatt cttgccaggc aaaccggtat cgcggagtat accgagagcg
    10741 ggatcagtct ggatgcgctg gtcactgccg gtttgctggt taatatcttt gttcccaata
    10801 cgccgctgca tgacggggca gtagtgatta aagaagggcg tctgagcaaa gcgacctgcg
    10861 ttcttccgct gagcgagaat acagaattac cgggtgatct cggcactcga catcgtgccg
    10921 gtctgggcat tacagaggta tctgatgcca ttgcggtcat cgtatcggag gaaaccggta
    10981 aaatatccat ggccatcgat ggacgcctgt acaggccgtt gcagcctcag tatctgcagg
    11041 aaatgctgac ccgggaatta ttggcggctc ctgaaaaaca aggtctttgg aggaggtggc
    11101 tgtcccgatg aacaaccctg acgagcgcca taaaaaatcc ggtataaagg ttttgtcctt
    11161 gctgatggct gtcatgttgt ggttttatgt tgtcaatcag gggggtgcag cgaccgggca
    11221 gaattatcaa cagaccaaac tgaattatta taatctgccg gccggactga ctgtcaacgg
    11281 gcctgaggcc gtatcagtaa ggatatgggg gagtgcccgg gacaatggca acgtgattgc
    11341 ttatgtcgat cttaccgggt tgcagccggg cgtataccac ctggctgtta aggtcaagcc
    11401 cctgcaaggg gtgatgtttg ccaccgtgca gccggacaag gtgagcattg aattgaaaag
    11461 ccaggcggat cgaagtttta ccgtccaata tgaggtggac cggaaccttc ctgagggcaa
    11521 ggaacttcat gatgtaacgc tgtcacctgg ccaatgcatt gtcaaaggag agcaggatgc
    11581 tgtcagcaag gtagcttcgg tgcatgcccg gatcaatgtg ggggatacaa ccggactgat
    11641 cagccagcag gcggtactgg ttgccagaga tgcacaaggc aaggagatca gcagtgggct
    11701 ccaactgatt ccgactgttg taaatgtcta tgcggtggca gatagcaaga agattagcaa
    11761 agagctgaaa gtgaaactaa aatttaaagg agatcccgct gccggttacc aactagggac
    11821 agccgtggca gatccagcaa ccgttacggt tctgggggag gagactcgtc tgaataacat
    11881 ggaggagatc agcagtcagg agatagatct ggccgatcac cgggtgagct tcacgcagat
    11941 ggtagcgttg ccggcactgg agggaatgat tatttttcca acgcaaatca aggtaatggt
    12001 taatattgaa ccggttgaca gtgaggttga accatgaagg taagagtgcg tgaaatacgc
    12061 ttgtcactgg aagaagatga aagccgcctg ccgctgttga ttgccagtag actggagatc
    12121 aatgtccgcc acatcaaaca ctggaaactg gtcaggaagg cggttgatgc ccggcgccgg
    12181 agtatctatt tttcctatac cattgatatt gaattagcgg aaggaatcac aattgcttcg
    12241 cagctattga ctcaaccggg gatcagtctg ctggaagacc aaccaaccgc acagctgatg
    12301 tccggcagca accgactggc cgttgctccc ctgattgttg gagctggtcc ggctggcctt
    12361 tttgcggcgc ttttgctggc ccgccacggg tatcgtccga ttgttatcga acaaggccag
    12421 gatgtggatc aacgggtgca ggcggtggag cggttctggt caggaggcgt attggacaga
    12481 tgttccaata cgcaatttgg agagggcggg gcgggaactt tttctgatgg aaaactgacg
    12541 actcgcattg gtgaccagcg agtcgattat gtactgcgaa cgtttgctga attcggggct
    12601 gatcccgaga ttatgtatgt aaaaaaaccg catgtgggga ccgaccggat tcgccaggta
    12661 gtaaaaaaca ttcgccgcga agtgattcgt ttggggggtg aattttactt ttctgcgcgg
    12721 atgacggaca tcttggttga acaaaaacaa ctaaaaagca taataataaa caatgaggct
    12781 gaaatcagct gtgaggttct ggtactggca gtgggtaaca gtgcccggga ggtctatcac
    12841 ctgttgcatc gtaaaggagt cagcctgatt cccaaagctt ttgctctagg ggtcagagtg
    12901 gagcatcccc agcatcttat tgacaggatc cagtatggac aatatgccgg tcatccccgc
    12961 ttgccggtgg cagattacca actcacctat caggatcgac ctacgggaag atccctctat
    13021 acattttgta tgtgcccggg cgggagagtg attgcatctt cgtcagcacc tggagaagtg
    13081 gtggtcaatg ggatgagtta cttcagccgc gacagcggat tggccaacag tgccatggta
    13141 gtcacagtgc atccggagga ttgggagaat gatcccctgg gcggcgtgca tctgcagcaa
    13201 caactggaac aaaaagcata tgcaatgggc ggagaaaact atcatgcacc ggcacaatat
    13261 ctgcatgatt ttatggctta ccaggcttcc ggcaatttaa acaattcaat ggccagttac
    13321 agaccgggtg taaccggggt caacctctgg cagttgttgc ctcaggaaat cgctgaagtc
    13381 atgcggcggg gactattgca ttgggataaa aaggcccctg gtttcatcag tgaagaggcg
    13441 gtaatgaccg gagtggaaac ccgcacttca gcccccttgc gcattgaacg tgaccagacc
    13501 atgaacgctg tttctatgga aggcatctat ccttgcggag aaggagcagg ctatgccggc
    13561 gggattgtca gcgcggccgt ggatggcttg aaagtggcgg aaaaaatcat atcccaatat
    13621 gaacggccaa attttatgcc gttgattaac aatcaccagg tacaacgagg cagccagtta
    13681 ccgaatgagg aaagaaatac tttgccaaat acgtaaaaaa atcataccat ctatataatt
    13741 gaactgatac aagcaaatcc aaaaacagtg tcatggttca cagccatgcg gacggggagg
    13801 cacaatacat gggtattctg tttgggacag acggtgtaag aggtatagcc aatttggagc
    13861 taactccgga aatggctttt gatctgggta gggcaggcag ttttattttg gctcagcagg
    13921 acagcaattc ccgaccaaga atcatggttg gcaaagatac ccgggtatcc ggcgatatgc
    13981 tggaagcggc tttgctggcg gggatctgtt ccaccggcgc ggatgcgctc accctgggcg
    14041 ttattcccac tccggcggtg gcttatctga cccagcagta caatgcgtcc tgtggggtgg
    14101 ttatctctgc ctctcacaat ccggttcagg acaatggcat caaatttttc ggtcccaatg
    14161 gctataaact gccagatgaa acggaagcgc agatagaaaa actggttttg cagggaaccc
    14221 gtgaactggt caggccgcag ggcgcagatg tgggcaggat ttatgatgtg gccgaggcca
    14281 ccaatcgtta tctggccttt ttgggcagtt gcacccaaga aaaagacttt agcaatctga
    14341 cggtggtcgt agattgtgcc cacggagcgt cttatcaggc agccccacgc ctttgggaaa
    14401 gcttgggagt aaaagtgatt gccatcaatg atcagcccaa tggtatcaac atcaacgatc
    14461 gctgtggatc gacctatacc gcttctctaa aaaaaaccgt cattcatcac aaggcagatc
    14521 tggggatcgc ctatgatggc gatgccgatc gttgcatcgc tgttgatgaa cgtggcaatg
    14581 agctggatgg tgattttatt atgaccattt gtgcgcttga tatgttccgg cgaggagccc
    14641 tccaacctaa ccggattgta gctacggtga tgagcaatat tggtctggac atcgccttga
    14701 aaagagaagg cattttggta gacagctgtg ccgtgggaga tcgttatgtg ctggaaagaa
    14761 tgatggaaac cggagcattg ctgggcggcg aacagtccgg acatattatt tttcttgaac
    14821 atgccaccac cggtgatggc ttgctgagct cgctcaaact ggccgaggtg atgaagagga
    14881 caggtctgcc gctttccgaa ttggcccggg agatggaaaa acagccccag atgctggtta
    14941 atatcaaact ggaagacaag gataccattt tgaatcatgc cctggtcctg gaagcgatgc
    15001 aaaccgctga aagaaagctg ggcgaacagg gcaaactggt ggtaaggcct tccgggaccg
    15061 agccgttggt aagaatcatg gcgcagggtc cctatcagga tcttttacag gaggtgatcg
    15121 atgagattcg tgcgtccata gaaaaagccc agcaaaaata atcggccggt atggccaaaa
    15181 agcgcctgac ccgctgcagc gcgggtgacg aggtgagggt ttatcgattt ttcggcgggt
    15241 gccctccggt tgccacgatc gatagcagag tgacaaaaag catagcgata tgtgtacaaa
    15301 accctctgca ggtggaatgt tggtacgatc caaatgagat ttgggaggtt ttatttaatt
    15361 atgtgtggaa tcatgggtta tatcggcagc ggagaagccg tgccggtcat tatagatggt
    15421 ctcagcaaac tggaataccg gggttatgat tcggccggga tttcttttta tcaaaatggc
    15481 agactgcaac tggccaagaa aaaggggcgg ctggcggaac tggtcaaatg tctgggtgaa
    15541 tttccctcgg cccatctggg tatcgggcat acccgctggg cgacccacgg tgtgcccagt
    15601 gaccgcaacg ctcatcccca tatggattgc agcgggcgca ttgccctggt gcacaacgga
    15661 attatcgaaa actatgccag tctgcgcaaa cagctgatgc tggaaggaca cgactttgtt
    15721 tccgaaacgg ataccgagat cgttgcccat ttaatagaaa aattctaccg tgattgttcc
    15781 ctggaagcag cggtacgcga ggccttgcaa catttaaaag gatcctttgc ggtagccgtc
    15841 atttcttctg atgagccgga caaaatcatt gccgccaaaa aagacagccc gctggtggtg
    15901 ggcgcgggca acggagaaaa ctatctggct tccgatattc cggctattct gggcaaaacc
    15961 aatcaggtat atatcattga agacaaccaa ttcgtcatcc tgaccaaaga cgcggtcagc
    16021 atcactgatt tcagcggcca gccggtggaa aagaaactgt tcccggtcac atgggatccg
    16081 attgcggcgg aaaaaggcgg ctatgaacac tttatgctca aggagatcca tgagcagccg
    16141 acggcgctgc gggaaacctt gcggggcaat gtcaaggaac agatcattga cctcggttat
    16201 ctggatattg atcccatgtt caaagacgtg gagaaaattt atattgtagc ctgcgggacg
    16261 gcttatcatg ccggcctggt gggacgacag gcgctggaaa aactggcccg catcccggtg
    16321 gaaacggata ttgcttcaga gtttcgctat cgggacatcc tctggaatcc ccgttcagtc
    16381 atgattgtga tcagtcagtc aggagagaca gccgataccc tggcagtgct gcgggaagcc
    16441 aaacggcagg ggataaaggt gctggcggtg accaatgtcg ttggcagcgc tgtttcccgg
    16501 gaagccgacc gggtcatcta cacccacgcc ggaccggaaa ttgcggtagc ctcaacaaaa
    16561 gcttattcaa cccaattgtt gctcatgtac ttactggctt tgcatctggc caacctcaga
    16621 agcacctgca gccgggaaac gctgcaggag ttgaccgccg atttggagat catggatcag
    16681 aaagtggaag cggtattcag ccaggaagaa aagattcgtc aactggcgga aaaatattat
    16741 caggtggaaa gcaccttttt cctgggacgt ggctttgact atgccgtagc catggaagga
    16801 gccttgaagt taaaagaaat atcctatatt catgccgaag cctatgcggc aggtgaatta
    16861 aagcacggcc ccctggccct gatcgaggag ggcgtgccgg tgttggccct gattacccag
    16921 gatcaactgg tggaaaagac catgtccaac atcaaggaag tgaaagctcg cggtgcaatc
    16981 gtggtcgcca tctgcaagga aaacctgcag gaagcctgcc aggaatgcga tgagcagatc
    17041 attctgcctg atgtcaatcc cattttggca ccgattgtag gggtcattcc cttgcagata
    17101 ttctcctatt atatggccat actgcggggg gcagatgtag ataaaccaaa gaatctggcc
    17161 aagtcggtaa cggtggaata atataaccaa cagaagtgac aaaaaatgtg ggaacgatga
    17221 tacccgggaa ttttagttaa ggttcccggg tatttttgac catgttataa tagctccata
    17281 acaatgaaac aataaattga gacatttagg cggtgttgac atgacaaaca aatctggttt
    17341 cgatcttgtc ctggaaaaca ttgattttcg caaatttgcc gacacccttt cggtgattat
    17401 ttttgcttat gcagataacc acaaatatat ctacgtcaat ccggcttttg aaacggcgct
    17461 ggggtattcg ctggaagagc tgctgcaaat gaacttttgg gacgtgtgcc atccggatta
    17521 caaggatttg gtgcgtgagc gggggcaggc ccggctgcgc agggaggccg tacccgccaa
    17581 ttatgaaatc agagctttgc gcaaggacgg cagtgattta tgggtggatg ttttttttag
    17641 cgtgacccag gttgccggga agttgatcag tattgtgggg gcttatgata tcaccgaaag
    17701 aaaacgattg caggaagccc tggtaatgag ccgggatcga ctggaaatgt gtgtccagga
    17761 acgtaccctc gaactgagcc agaagaatct tgaattgact ttgctaaatc gtaatctcaa
    17821 taccgttatt atgaatatgt cagatggggt cgtctttctt catcaatcag gtgactttga
    17881 actgctcaat cccgattttc acaagtcctg gcaggaaagc catcgcggct ttgaagaaaa
    17941 ttttcgtgaa tggtgcagcg acccggacaa caaatacatg aaacgcctct ttgtttcggg
    18001 aaaagcgttt caggaagaag agattgttat tcccatggca ggtacttccc tgcgattttt
    18061 ggcttcggga acacccatcc ttagcgaaca aggaatggtc aatacggcgg tactgattat
    18121 tcgacctctg aaggaagtcc accacatggt cagtcgtttc agtggttata ccgcccggtt
    18181 tcattttaag gatattatta cctgtaaccc acaaatgctg gagattattg aggtggccag
    18241 aatggcagcc cgcaatacca gcaatgtttt gatcgaaggt gagagcggaa caggcaagga
    18301 actttttgcc caagcgattc ataacgccag tcagcgttcc gccggggcgt tcgtggcgct
    18361 aaactgcggc gccataccca gagaactgat tgccagtgaa ttgttcggct ataatgaagg
    18421 tgcttttacc ggtgctaaaa aaggaggcaa tcctggcaaa ttcgaactgg ctgacggtgg
    18481 gacactgttt ctcgatgaaa ttggggatat gccgctggaa ctgcaggtag ctctgctgcg
    18541 cgccattcag gaaaagagta tcagccgtat cggcagcaac cagattatcc cggtggatgt
    18601 gcgcatcatc tgtgccacca acaaaaatct ttctgaggag atgcagaagg gcaattttcg
    18661 tcaggacctg ttctatcgct taaatgtcat tgatctccac atcctgccgc tcagggaacg
    18721 ccccgaggat atcctgctgt tgtttcatca tttcctggct caattgagtt ctgcaactca
    18781 ctcggttctg gaagaagaac tgaaaaacag tctgttgtct tatcaatggc ccggcaatgt
    18841 ccgagaactg cagaatgtgg cggaaagaat cgttaacctt gatcatttgc gcgacatgcc
    18901 gctcaacctg tcctggcaac acgctggact tctcgaagag aagaaggccg gtcatcattt
    18961 tagtattcag caggcccaga cgaaatacaa gaatgaaagg gtagagcagg aacgccatcg
    19021 cgtcattagt ctgctcaaac agtacgatgg aaacgtcagt cgggtagcca gggaaatggg
    19081 catctctcgc agtaccctgt atcgcaaaat gaaaaatctg agtttttaac aagagtgctg
    19141 caagggtgtt ttgttgtgtt cgaaattata aaacaatacg atacagataa cgacactttg
    19201 tacaccctgg atcctgtgat agagggaccc cgggtgttgt tttttccgat gtggcaggat
    19261 aatgtaattg gccgcggcga attggcatgt attttgcgat attaatgagt tgatgtgcag
    19321 tatggaatgg cttgcgtcaa caaataatat ggggggaggt tggaacagaa tctccacctg
    19381 caagaaagag gatagtttat aaaaatacaa agacaaggag tgaagcaaat gacagaaaga
    19441 agatggcagc aatggtatga cccgggggta ccgactacct gttattatcc gctgaccacc
    19501 atgaaggatg aattcatcaa atgggtcaga gccaatccgg acaaaccata catctactgc
    19561 aatgatctga cgtacagcta ccaggaaacc aatcatatcg ccaagaaact ggccaacgtg
    19621 ttgctggaat tgggtgttaa gaaaggagat cgggtatcac tggttttgcc taatataccg
    19681 gaatttattt tttccgctca tgcggttatg aaaaccgggg ctattattgt accggtcaat
    19741 cctctttata cggttccgga aatggtttac aatttttccg acagcggttc agaaacggtt
    19801 attgtctatg gaccagtagc acctcgcatt ttagaagtaa tgcgttccgg ccaaacccag
    19861 ttaaagaaaa tgatcgtcat gcaggtgccg ggcaaggaag tagcccttga agctgcccct
    19921 gacatccttg attacgcctc cgttatcgaa gctgccagcg acctcgaacc ggagatccat
    19981 ctggacaaca aggataccgt cattctcatt tataccggcg gaacgacagg ggttccaaag
    20041 ggatgctgcc tgaccaattc caactttgtg gctttctata ccgtttttat tgaatgggcc
    20101 agagccatgt tcccccctca ggaactgcgg actttatgca cagtgccgct ttaccatatt
    20161 tatggcttca acatgcagat caatgccaat ttggcaagcg gcggcaccat gattctggta
    20221 ccggaagtaa ccccggacaa cgtcctggat gccatcgatc gcttcctgcc cacctattgg
    20281 ccggcagtcc cggcactgat ccaaatgctc aacatgcatc cccgtctgcc tcaatccaag
    20341 gccagttcga tccgcttcct cctgtccggc tctgccccct tgccagtgga agccatgaaa
    20401 aagtttgagg agctgaccgg ttccacgatc attgaaggat ttggcggatc ggaaaccacc
    20461 aacggcgtta tggtcaatcc caagggcaaa cgcaaaccag gcagcgttgg cattcctttc
    20521 ccggatatgg atgtccgcat cgttgatctg aaaaacggga cggaggaagt gccgatcgga
    20581 gagccgggcg aattgcactt aaaaggccct ttgatcatag aaaagtactg gaacaaaccg
    20641 gaggaaaccg ccaatgccat caaggatggg tggttcaata ccggcgatat cgccaccatg
    20701 gatgaggacg gttatatcac catcgtggga cgtaccaagg acatgatcat ctccagcggc
    20761 ttcaatgtct atccccgcga catcgatgaa ttgctttata ctcatcccaa agtgctggat
    20821 gccggagcca tcggcgtacc tgatccaatc cgcggggagt cggtcaaggt tttcgtagtc
    20881 ctaaaaccgg gtgaaaccat gaccgaagag gaggtcatcg agttctgccg tcagtccctg
    20941 gcagcctata aagtgccaag atatgttgaa ttcctggaag atattccccg taccaatatg
    21001 aaaaaagtag atcgcaaagc cttacgggaa atggaagcca cccgtcagaa ataggtctgt
    21061 ttatcaacta taccgatacc cgaatgcata taaaggctgt cccggttgtt tttacaacgc
    21121 gggacagcct tgtttacgta agaaggagac tgactcacga ttattttgcc agatgaagga
    21181 atccttttat tgcttatcga aagaatctat tcataaatgt agatcggatt acggtattta
    21241 tcggctaagt catatttaag tgacaaatat gaaagatatg taagcaaagg agtgaaagca
    21301 tacatgaata tagatgcgaa aattgccgag ctggcggaaa aaaacaaaga tgtgcctttc
    21361 tataatttta tcggcctgga gtatggtaaa gcaggggaaa actatgccga attgatgctg
    21421 gaggtaaaaa aagaaaccat gaatgtccac ggggttttgc tgggggttat gtattatgcc
    21481 ctgtgtgatg tttctacttc tgtggccgtg gaacacagtc tgccggaagg atattattat
    21541 gtaaccaacg acctcaatgt ctccattttt tcttccgtca gttccggcca ggtgagagcg
    21601 cgggcagagg ttttaaaaac cggcaaacgg ctggcttttg ttgaatcccg catctataac
    21661 gaccgggatg aactgctggc ggtgggacgc gtgaccaaga cccgcttggc caaacaccaa
    21721 taggtcatat ttattaaggc aaaataatct ggtgagaggg gaaaatggat gagaaatccg
    21781 atgtttgatg tattaaaggg tctgggtatc atttctgtgg ttttcagtca tgtttaccgg
    21841 ggagggactg acccgctggc agtatttatt cgggaagttg ccatgtggag tgtacccatg
    21901 ttcttcatcg tccagggtca ttttatgcaa agcggtctgc agaagggttg gctaacatct
    21961 tcctgggata aaatcaagaa gagctatgta ccttacttgt attgggcagt ggcctatggt
    22021 gcgttttatt atttcaccat tggcaaaact ttctcggcca tggacttaat cctgggcaag
    22081 actgccctgc atctttacta tatgttttac tacattatct ttgccgtttt tatgccgctg
    22141 ttgtatttcc ttcccaagct gtggcgggtg gcgattcttt ggctgatgat tctggtcaat
    22201 atttactggg tgctaatgct tgagcttagc cgcttgtatc atttccactg gattagttat
    22261 tcgggaccgg tgcccatgaa atggtgggga ttcattgcca tcggcatgct ggtggccgag
    22321 tacccgcaaa tcaaagaata tatctgccaa catgcccgcg cctttttgat cggcggcttg
    22381 gcggtggcag ccgttgggac tattattcct tatatcaatc atacaacggg ctatttattt
    22441 aacaaagttg aaattttccc gctgtctatt ggcctggtcc tggctctggc catttattac
    22501 agccgggatg atgcttttgg caaacaaacc ctggcttata tcggggagag gaccttcggc
    22561 atctatctgg ctcatttctt catggtggat tatttgcgcc agactttgct gccaggggat
    22621 cgaaccctgg tggtcatcat cattctggtt atctgtatct tggttaagga caacaaagac
    22681 cgcatcaagg cagcctggaa agaaaaacgc agcggtaaac agaacgttgc ctgaacgcga
    22741 gagaataagg attaggggat gataaaaatg tgggccaaga tcagccgttt ttacccgccc
    22801 tggctggatg tgatctgcgt tatctgtttg ggatttatct ggctctatac agccgttcgc
    22861 tacggcctta tggctgatca aatccccact cacttcggtt tcagcggaac acccgatgca
    22921 tggagcagca aatctttcgg ttctgttttt atgctgcttc tgataggaac gatcgtgtgg
    22981 atgtccatgt ttttgataaa ctatttttta atcatcaaac cggaagatcc gcgtaaatat
    23041 attaatcttc ctcagggcag gaaagatgcc atgagcctgg aagaggttga agcgctccgg
    23101 cgaatcactg ttcaaggcat ggccataatc aatctgaccg tggtgctgat gttcgttttc
    23161 attcaatatg gcatggttaa ttccgcgctg ggcctgcaaa atgggttggg ctggggggtt
    23221 aatgtgctgg tcggtattct gctcatcgaa acggcctggc tgacctggaa aactttttct
    23281 ttgagcaaaa atgcatctgg tgctaggaga taactcccgc gaatccatct tgttgtagaa
    23341 gatgattatg attggagaaa taacttaaaa tcccaatcaa agcgaataca agctaaagaa
    23401 tcacggaatg caagcattat taacctttgc gggggacgct gtttattcaa tggtcataat
    23461 tgttgaaaag ccggtcaact caaaaatttc tttgaccgcc ggaatcattc cggtgatttt
    23521 catggtcgtt ccctgggcgt tgaccttttt ctgtgctgtt aaaaacacac gcagaccggc
    23581 gctgctgatg tatttaagag aagaaaggtc aaacaccagg tttgccttgt cagcggcaaa
    23641 aatcttctcc agttcgctgg ccaattctgt atgagtaacc gtgtccagcc gtccccccag
    23701 ggcaatcgta acggtattgt tcttttgtgt ttttgtgatt tccatcgtat atcctccccg
    23761 atttgattct cataaaataa atacttctta aaggataaac cttgagaagg aagaaacgca
    23821 aattacaacg aaattctcct ttaactgaat gaatgattta attgtatttt atgcgaataa
    23881 aaaagcgagg taaatatgcg taaaaaattt ttgatttggc ttttattatt tatgatggtt
    23941 gcttttatgg cggcatttta cgtatcatat acgattcaga gcaggcaagc gtacaataac
    24001 gcggagaaat tgatattttt aaaaattaat gatgtgatca aacaaatcag catcaataat
    24061 agaaatctgg ctgatatcag atgggaaagc gatgccaatg ccctcgccaa agcgcgggct
    24121 tttgcctgga tgctggaaaa agatcccgat ttggtcaatc attatgaaga actcgaaaaa
    24181 attcgccaat tgattgatgt cgacgaactg catataacag acggggaggg gatattgatc
    24241 tccggtactg ttaaagagta ctacctctat agatttgacg cggataagca atcggcagca
    24301 tttttgccgg cgattcatga taaaacgttt gaactggctc aggatcctca gcccaaaggc
    24361 ataaacggag aaatgtttca gtatgttggt gtagctcgca aagatgccga cgggattgtt
    24421 caaatcggat acaggcctga aaagttagcc aatgccatgg aggttgctga tattaaaaac
    24481 ctggctcccg gatttagggt gggcaattca ggcagcgtaa tcattgccac caagacaggg
    24541 gttattgtca gtgcagctga caagaacttt attggaaaaa cagtttatga attttgtcac
    24601 ggttatgatc ccaagggaac tagtggcagt tttatcagag atgcctatga cggcaggagt
    24661 ttatttgcct tcaaagtgac cggtgactat atgattcttg gtaaattgcc tgctgatgaa
    24721 atgtatttaa gccgtaacag cacaacgacg gtaatgatat tatttaatat cctgcttttc
    24781 tccctgattt tttttctggt tgccaggttg gtgcaaaccg ttgtcattga tggtatcttt
    24841 gccgtgaata aatcgctggt gcagataacc caaggcaacc tcgatgagaa ggttgaagtc
    24901 aacaccagtg aggaattcca ggcactttct tcagggatta acggaatggt cgaggcgctg
    24961 aaggacgcca ttaaggaggc agccgcccgg attgatgatg aactggcttt tgcccgggcc
    25021 attcaactgt cagcgctgcc caaccatttt ccggcttttc ccgataaatc agaaatagat
    25081 atatttgccg atatgcatcc ggccaaggaa gtaggcggcg atttctacga tttctttttt
    25141 gtaggcgaaa atcagctggc ggtagtcatc gctgatgtat ccggtaaagg gattccggct
    25201 gccatgttta tgatgattac caaaaccctg attaagaatc tggctctcac cggcatttct
    25261 cctgccgaag tgtttattaa tgccaatcag tcattgtgtg aaaacaatga tgcaggaatg
    25321 tttgtgacgg catttatggg cttgcttgac tgcgagactg ggattttgcg ctatgtaaat
    25381 gccggacata atccgccgct gattaaaaaa accgatggga cggctgcctg gctgcaggtc
    25441 aaaccgggtc tggtgctggc tggcatggag ggcgttacct atattgaaga agagattagg
    25501 ctacagccag gcgatatgtt gttcttatat acagatggtg ttaccgaagc ctgcaatcat
    25561 gcgttggaat tatacactga ccatcgcttg ctggaggaat tgaatcact
//
LOCUS       DAOTHZ010000028        25240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2284_length_25240_cov_7.871789, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000028 DAOTHZ010000000
VERSION     DAOTHZ010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 25240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25240
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2284_length_25240_cov_7.871789"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..453)
                     /locus_tag="P5549_04980"
     CDS             complement(103..453)
                     /locus_tag="P5549_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRW12457.1"
                     /translation="MSQTDCLFCRIINGEIPSEIVYQDDYLIAIKDIEPAAPVHLLLM
                     PKMHITSLNHVGEQQIEILGRLQLLAAQLAESLGIAEQGFRLVNNCGVGGGQAIFHLH
                     YHLLGGKEMGWPPE"
     gene            complement(585..1757)
                     /locus_tag="P5549_04985"
     CDS             complement(585..1757)
                     /locus_tag="P5549_04985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW12458.1"
                     /translation="MAAFTLSKEENQLIREVHELSISKIAERGAYLDRVGDDKVDWKI
                     PEYLAKMNLLAPIVPKEYGGRELSMMAATSIIEEIALGCAGAAAIVVMNMYALTPLLI
                     AGSDALKEQFLPDLCKSRPHLACLAISEITPEHDLDRSSRPREDITRITTTAEAQDGQ
                     ITLNGIKDFVMNGGASDFVAVLARSKESKQKSHLQMYLVPGRTPGLETNRVLNKVGMR
                     SSHTVQLAFRDVRIPLEYRIGRDGSGYFLFTQIFDRNMPLVAAIGVGIARAAYELTLQ
                     VVRTNQMLNRNSTVNQYVSSTLVDMSTEIDAARLAVMRAAHYVDTDDNYSRAAYMSKI
                     YATRVAQWTTFQAVEIIGRMGFTVGHPAEKYLRDAQMLSAIAGSEHLHKQVVSQQL"
     gene            complement(1787..2956)
                     /locus_tag="P5549_04990"
     CDS             complement(1787..2956)
                     /locus_tag="P5549_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW12459.1"
                     /translation="MFRSTISAQDRDLVMMVRDLVRQEIAPKAVVYDAQGNENFDWSA
                     IDLLAEHNLLAPSISPEYGGRGLSHLSAAMIVEEIAVACAGVAASAVANMHAVSPLVT
                     SGTETQRQEVLPLLTSGKACLGSLAMTENRPNLNILVENAQIEIINSSVTGFWQEGCL
                     ILNGFKDYVLNGQVASFITLLVTLDGPEKKTGLQIVVIPLSLPGIRTGAVRQKIGLKY
                     CNSCEVIFENVKVEPRYIVGKPGSGFLIFMQNLDRMVPYVGAMSLGVARAAYETALAI
                     AKKRYIMGRPSFEETAISFALVDMAAQLNAARQSVYLASWLIDQELDSSQASSKAKIL
                     STRAAHDISSKAMEIVGGRAYVKGYPSEIYLRDAEMLSIIDGSEQFHKSLIAAQL"
     gene            complement(2968..3330)
                     /locus_tag="P5549_04995"
     CDS             complement(2968..3330)
                     /locus_tag="P5549_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW12460.1"
                     /translation="MGFKEIGKKLQLAREEAGLSQEQLANMLGCAQSTLSNYEKGKRR
                     LYLTQLENIAELLDKPIEYFMESSSSHTETPAVNHIASSEDDDQELLQIINTLYTLPR
                     ERRRSVMDYIIWQKTRSN"
     gene            complement(3396..4715)
                     /gene="mtaB"
                     /locus_tag="P5549_05000"
     CDS             complement(3396..4715)
                     /gene="mtaB"
                     /locus_tag="P5549_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRW12461.1"
                     /translation="MKKIAFHTLGCKVNQVDTEGLIEDFSHRGYEVVDFHEIADIYVI
                     NSCTVTHVSDRKSRAMVRRAVRRNPGALVVATGCVAQVDPAQLAAIEGVKLVVGNRDK
                     HNLASLIEDYLRQHRSDQQIIVNPIRREDQPQKILYHCQHQRTRAFVKIQDGCQSFCS
                     YCIVPMARGPVRSKQPAVVLQEVEQLIRLGYKEIVLTGIHTGFYGIDLPGWTLSRLVN
                     AILEEIAGTYRIRLSSIEPLEVDDSLLSLVAADQRMCRHLHIPLQSGSNKVLQAMGRR
                     YHRDDYRKLIRTAADRIPGMAFTTDVMVGFPEETDSDFQDTLELIGELPLVDLHVFKY
                     SRRKGTKAAELSGQIPEPKKNQRSEKLLEIARNKKLAFAKCFLGQRFDLLVEEKSGKD
                     KYIGLTDNYLEVTVQSDHDLCGQSVAVEIASADSTGLRGNICIEPPQ"
     gene            complement(4762..5571)
                     /locus_tag="P5549_05005"
     CDS             complement(4762..5571)
                     /locus_tag="P5549_05005"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRW12462.1"
                     /translation="MDGDRCPQAIDVFPGGKPTNMHRFYVNPQEIKGNQVRLDQEQSR
                     HIEKVLRFVPGDRIMVFDGLGREYEVVLSRVVAGQAEGEIIRRLPVGDEPVMRVKLVQ
                     GIAKGDKMDFIIQKAVELGVDAIYPLSSQHAVVKLEKERALKKQERWRVIAREACKQC
                     RRNRIPEVYPPLSLASMLEIIDAQPAIMLYENEKQQGFKELLKEKCPYLLGEQELFLI
                     IGPEGGFAETEVEQARSQGVMTAGLGPRILRTETAGLAALTIVLYELADLG"
     gene            complement(5541..6461)
                     /gene="prmA"
                     /locus_tag="P5549_05010"
     CDS             complement(5541..6461)
                     /gene="prmA"
                     /locus_tag="P5549_05010"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRW12463.1"
                     /translation="MNWKEYAVITEGQCVEAVAGILHNLGSGGVSIVPAHSDVGQIIA
                     GTSDIPALPAYGKHDLVTVKAYFPEARDIDAQLQEALYRVEENFHVVCQLLLNEVKEE
                     DWEESWKKYYHCFKVGDRLVVKPSWEEYSPQPGEMVIELDPGMAFGTGSHASTRFCLH
                     FIDEHVRGGERIIDAGCGSGILAIAAAKLGAARVLAMDIDELAVKIARANVQMNRQED
                     IIAVEVRDITEDLAGFKADYIFANITAEVVSLMIPRAARLLSAGAYLFCSGIIESRWP
                     QVRQQLEDHGFIIDRVLQENEWMGIAARRQ"
     gene            complement(6474..7607)
                     /gene="dnaJ"
                     /locus_tag="P5549_05015"
     CDS             complement(6474..7607)
                     /gene="dnaJ"
                     /locus_tag="P5549_05015"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRW12464.1"
                     /translation="MADKRDYYEVLGVSRDATPDQLKKAYRQLAKKYHPDVNKDDKDA
                     ADKFKEVAEAYEVLSDEQKRSAYDRFGHDAFDPTRGGAGGFGGFGEGMGGFSDIFDLL
                     FGTAGGGGQRRRSGPQRGSDREVRLDIDFEEAVFGSEKDFDLHRVEKCGTCKGNGAEP
                     GSPIKTCPQCKGTGQMRSAQSTPFGRFETVKTCSRCQGEGKIIEKPCSACKGTGKVKK
                     KRTINVKIPAGIDTGARLRMQGEGELGNNGGPSGDLYIMIVVQPHEKFAREGYTLITE
                     VEIDFVQASLGAEIEIPLLGGMSHKLEIPSGSQPGDVLTIKGKGIPHLQGQRNGDLKV
                     YLKIKIPTKLTKRQKELLMGFYDDHDNKDGKKGIINKLKDAMG"
     gene            complement(7756..9552)
                     /gene="dnaK"
                     /locus_tag="P5549_05020"
     CDS             complement(7756..9552)
                     /gene="dnaK"
                     /locus_tag="P5549_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRW12465.1"
                     /translation="MGRVVGIDLGTTNSVIAIMEGGEAVVIANAEGERTTPSVVGFSK
                     TGERLAGRVAKRQAITNPDRTVTSIKRKMGTDYKVNIEGKTYSPQEISAMILQKLKTD
                     AEAYLGEKITQAVITVPAYFTDSQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKE
                     ENQTIMVFDLGGGTFDVSILDIGDGTFEVKATSGNNRLGGDDFDDRIINWLNQEFKAS
                     NGIDLNNDKMAMHRLKEAAEKAKHELSGMMSTEINIPYITATQEGPLHLERTLTRAKF
                     NEMTADLVDKTMGPLKQAMKDAGLKPAEIDKIILVGGSTRIPAVQDAVKGFLGKDPFK
                     GINPDEVVGLGAAIQGGVLTGDITDVVLLDVTPLSLGIETLGGVFTRVIERNTTIPTE
                     KSQVFTTASDNQPSVDIHVLQGERKMAEHNVTLGRFQLTGIPPSPRGVPQIEVKFDID
                     VNGIVSVTAKDLATNKEQRITITANSGLNDDEIDRMVREAELHAEEDEKRFKEIETRN
                     AADSMIYQADKTLKDFAGQVDEEDKRSIEEAREALQKALEGSDIDDISAKTEALTNAI
                     YAVSAKAYEKANPGNTADDGVFDADYNIPDED"
     gene            complement(9659..10231)
                     /gene="grpE"
                     /locus_tag="P5549_05025"
     CDS             complement(9659..10231)
                     /gene="grpE"
                     /locus_tag="P5549_05025"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRW12466.1"
                     /translation="MNNDNSYEAQEITLQSEAANGNEEVVQENNASEKSELEILQEQL
                     QSKTEEAGKYYDQYLRSLAEAENIRKRSQRDKEEYVKFAQLSIVKKLLPIIDDLNRGL
                     EAASAAQDFEGLKKGMEITTRNLLDLLRQEGVQAIECVGKPFDPQYHEPLMVVSSDEH
                     PENTVLEELNKGYVMHDRVIRPSLVKVSGK"
     gene            complement(10242..11798)
                     /locus_tag="P5549_05030"
     CDS             complement(10242..11798)
                     /locus_tag="P5549_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRW12467.1"
                     /translation="MKDNHLVNGEVDQKFQTLLSNANAARVISQAIEGTIGPKGLDIM
                     LVDKFGDVVISNDGATILKLMEVNHPAARMIINAAQAQQDEVGDGTTTATLLAGAMIS
                     VGTEQVLKGVPVTKVIQGITLGTERCLEVIESQNVRMLTLEDEKLLDVALIAGRGDME
                     VARLVIEGAKIVGEDKMFAPEFKFCDTVVAREAVESQVFTGVLINREPLNQEMPTSLK
                     KAKILLLNDALAPEAIDSEARGTENGFQYYLHSQEQYQQNLHKIAALGINLVLTDRSI
                     DDIAEQIFSDAGIIAIHRVSRVEMEKIARSSGARKIKRSALAKEAEQLEKYLGFAEKV
                     EVDQKAGHTLIYQGSGESMATILIGAATAELVEERERIARDAAAAVQAALLKGVVPGG
                     GSIEIWAAQHLNELAWELKGMSSYGVLCVKEALLKPFHCIAQNAGFNPLEKMEEVVAV
                     QMKTGSASAGIDCDSGEMIDTMASGIVDPTWVKMHAIKTAADVTVAILKINTIIKMTE
                     VETGKLLEME"
     gene            complement(11812..12837)
                     /gene="hrcA"
                     /locus_tag="P5549_05035"
     CDS             complement(11812..12837)
                     /gene="hrcA"
                     /locus_tag="P5549_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRW12468.1"
                     /translation="MNLDERKKLILESIIKDYVETAEPVGSRAIVRKHGLNVSAATVR
                     NEMADLEEMGYLEQPHTSAGRIPSEKGFRYYVDWMMEKESLSDEEMEVLDKLLHENLR
                     DWSDIAQGVGNFLAQVSNYTSFIIVPSIKFTQFQYLHLVPVQEGQALVLVVTDIGLLL
                     HRKIDVPKGITVSDLQSVADVFNQVFRGKRLSEMKRSHLQILRDDLKNRRRVIDKALD
                     AIDSLLENSGEEKVLISGALNILNEPEFKDLYKLKRILTILEEDELLRDILPESITQN
                     VDYRIGKENQSEDIREMSLVFTGYQTSGDMGKMGLIGPIRMEYWKAAGTLDSVRTFIE
                     DLLKKRN"
     gene            complement(12984..14129)
                     /gene="hemW"
                     /locus_tag="P5549_05040"
     CDS             complement(12984..14129)
                     /gene="hemW"
                     /locus_tag="P5549_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRW12469.1"
                     /translation="MTNNNQAGIYIHVPFCVRKCSYCDFYSRPLPDKDDLERYTSKLL
                     REIEQQAGQWANRCFSTVFFGGGTPSLLEPEQIHRILQGLRQHLSLLPQAEVSLEANP
                     ATLDASRLQALKSAGLNRISLGVQSFRDEELKILGRIHSVREARQAVEDIVASGFDNF
                     NLDLIYAIPGQNLDDWQYNLQSAVNCQPRHISAYLLQLEPFVPLARQISAGRYAETGE
                     DLQADMYDATVDTLIGQGFHHYEISNFAKPGSECRHNLIYWQAGEYLGLGTGAVSFRD
                     GQRWKNRETVSGLPDESDSGMIDIEILESMNQHDRFVDAVILGLRLTQGISRQQFIER
                     FGIDFAQEYHAILEKFRRDGLLIMEQDRLYLSRRGYFLSNQFMAELL"
     gene            complement(14119..15921)
                     /gene="lepA"
                     /locus_tag="P5549_05045"
     CDS             complement(14119..15921)
                     /gene="lepA"
                     /locus_tag="P5549_05045"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRW12470.1"
                     /translation="MQDIIRNFSIIAHIDHGKSTLADRLMQFTGALSDREMREQFLDK
                     MDLEREKGITIKLQPVRLNYRARNGKTYILNLIDTPGHVDFSYEVSRSLAACEGALLV
                     VDASQGIEAQTLANVYMAMDLNLEIIGVVNKIDLPGAEPDMVKQEMENVLGIDKDEII
                     PISAKLGTGIEDVLEAIVAQIPAPRGDEKAPLQALVFDSYFDAYKGAISYIRLANGEL
                     RKGDMIRMMSSGKEYEVIEIGVISPYMTQVEVLQAGEVGYMAAAIKNVADTKVGDTIT
                     NANQPAAKPLSGYKEVKPVVFCGLYPLENSEFDKLRDALEKLKLNDASLLYEPESSDA
                     LGFGFRCGFLGLLHMEIIRERLEREYDLTLLATAPSVIYRIQLTDGSEVYVQNPSRWP
                     DAQRIEHVMEPFVKASIMVPNEYVGTIMELCQEKRGTFINIEYITPHRVLISYYLPLA
                     EILFDFFDGLKSRTKGYASLDYELHGYQPSDLIKMDILLNGEAVDALSTIAHKEKAYY
                     RGRALVEKLRKIIPRQMYEVAIQASIGNRVIARESVKAMRKDVLAKCYGGDITRKKKL
                     LEKQKEGKKRMKQIGRVEVPRDAFMSVMDIESDK"
     gene            complement(16052..17122)
                     /gene="mnmA"
                     /locus_tag="P5549_05050"
     CDS             complement(16052..17122)
                     /gene="mnmA"
                     /locus_tag="P5549_05050"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRW12471.1"
                     /translation="MGAGKVMAAMSGGVDSSVAALLLIQSGYQVVGATLELLSDPGED
                     NPAGGRDGSVVEDARRVADQLGIPHYVLNDREAFEKKIIAYFCEEYLQGRTPNPCVRC
                     NQLIKFGDLLRQAQSMGFDYLATGHYARIQYDRQRQRYLLYRGQDRSKDQSYFLYLLN
                     QEQLAHSLFPLGGYSKADIRTIAGAMELTVAEKSESQEICFVTDDDYARLVEDRYPGS
                     ARPGPILNRQGEVLGQHRGLLHYTVGQRRGLRLAQGYPVYVLALDSRRNAVVVGRNEE
                     VFANGCSVVQLNWIAVENLTQPLEVEAQIRYKAQPTRAMIRPRRDGSVQVEFGEAVRA
                     VTPGQAIVFYEGDVVVGGGTIV"
     gene            complement(17134..18711)
                     /gene="murJ"
                     /locus_tag="P5549_05055"
     CDS             complement(17134..18711)
                     /gene="murJ"
                     /locus_tag="P5549_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRW12472.1"
                     /translation="MTNAGATIARAALFMMLMTAVARVLGFAREAVMMTLFGQNYLTD
                     AYRAAFSIPDFIYLLLVGGALSAALIPVFTTYLSNGREEEGWEAVSVVFNYIVLLLLV
                     LIAAAYFFTSPLIKLLAPGLPPAYAAMAVSLTHIMFIQTFFMALSGISQGILNSYNQF
                     AAPALGSVLYNLFIIILGIALFQQLGIRAFAIGVVVGAVFNFAVQIPALRRVGVHYHF
                     TFNCRNPGFVKIMILMIPVLLGLSVAQLNLFVTQNLASSVGAGMISALNLAQKIMNLP
                     VGIFGAAIATAVFPTLTAMTARGDMAGFRRHSSLGLRAIFLVTIPSAFGLAAIGEPLI
                     KLLFQQGQFTAAMAHTTFQVLLYFCAGLAAYAGIMVLNRSFYAMKDTLTPLMAAGATI
                     VLNVVLSLYLSGLMGVKGLALAISLAGIFNCLVLAAALRLKAGPIGGRVLMRSLTVAL
                     LGSTAMYFVVNYSIQLLLPWLPWADKMNQLLAVTAGVSLGAAIYGLIIYGFKMEETEL
                     VLKMVRHRIPGWGANQR"
     gene            complement(18833..19135)
                     /locus_tag="P5549_05060"
     CDS             complement(18833..19135)
                     /locus_tag="P5549_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12473.1"
                     /translation="MNKIFSSLLIIIVLGLVIAGLNTSSEGISSLTAEQRKPVLGLQI
                     EKQQINIIALGEEYHLTPEGISKTGQTVLRDVRQQYCSCVDYLQKIVTIFDVLFLQ"
     gene            complement(19132..20097)
                     /locus_tag="P5549_05065"
     CDS             complement(19132..20097)
                     /locus_tag="P5549_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRW12474.1"
                     /translation="MDFHRGFVDFLPRLRIIKVSVPFSGIAINQHQAEWLLGETCLSM
                     LGTEHFVDWGAEQMQTASIAENMIDSHMQVLAYATPVAAPVVEPALPQDVSSDQEAVP
                     SREQEEKTVAEGLFRGKKVLIYCTHSAESYIPDSGKARLDGKRGLINKVAENLQKALV
                     QQGLPAEFVNTIHDYPDYDQSYTNSRKTVKQILQTDKEILALFDVHRDSLPGTTKAPT
                     ITADGRKSARVLIIVGTDERKPHPEWKKNLAFAQALQAQGEKMYPGLIKGVTTKAGTY
                     NQEFHSRALLLELGSDYNTLEEANYAAELFADVIIQVLKEEIQPQ"
     gene            20411..20680
                     /gene="rpsT"
                     /locus_tag="P5549_05070"
     CDS             20411..20680
                     /gene="rpsT"
                     /locus_tag="P5549_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRW12475.1"
                     /translation="MAKSKTPAKRARIAEDNRLRNKAQKSKLKTSIKHYEASLTGDNV
                     DAAREDLIKVTSLIDKSVTKGVLHKNTAARKKSALAKKFNSLTGN"
     gene            complement(20755..21735)
                     /gene="holA"
                     /locus_tag="P5549_05075"
     CDS             complement(20755..21735)
                     /gene="holA"
                     /locus_tag="P5549_05075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRW12476.1"
                     /translation="MAQARGIYYFWGDEPYLIEQKIKDLLARIQKENGEEPEVVRLDG
                     DEMSSRELGETLQASSLFSFARVVIIKNPAWLGKNSRKAKKAEEIAHLLEDYLVSNPV
                     GQTLVICAAEHVAANPVAKILSRQAQGENIKAPGAKALGDWIKEKCNQLGLTVEPAAL
                     NLLVSSGQDMYYLENLIEKVSLIVKNGSIKVSDLNDELDSRQETSIFKLTDALLNRNT
                     AASLEAFQQLQEQGQPYLLMLHMITGQLVTLAKIKFWQEAGYSFNQIAEMSKQKDFVI
                     RKMQEKSSRFSAQDIQALFNKLLEVDISMKNQSKDPRLLMEILITDFCQR"
     gene            21913..23289
                     /locus_tag="P5549_05080"
     CDS             21913..23289
                     /locus_tag="P5549_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRW12477.1"
                     /translation="MTDRYDVIIIGAGPAGIFAARELCRNSSAKVLLLEKGRLVDKRS
                     CPVSEGADKCIHCQPCSIMSGWGGAGAFSDGKLTLTTEFGGWLSDYIGKQRLAELIEY
                     VDQVYLQHGASDTVYGQNEEEFETMRLHMARANLKLLPARIRHMGTDNTLKVLQNLKT
                     ELDPHITIKTGEAVESILVERHRAKGVRTKRGSYYAPRIICAPGRDGAQWFSEQMLKL
                     KIPLSNNQVDIGVRVELPAVTMQDFTDKVYEPKIVYNTKEFDDLVRTFCVNPNGHVVM
                     ENTNGIITVNGHSYAGSKSNNSNFALLLSQEFTEPFNQPIIYGRCIASLANMLSGGII
                     VQRFGDLKRGRRSTKARIAKGLVEPTLKAAVPGDLSLVLPHRYMVGIKECLEALDKAI
                     PGLGADHTLLYGIETKFYSARPQLSSSLKTCIEGLWASGDGAGVTRGLIQASVSGVVS
                     AREILSEI"
     gene            complement(23354..23683)
                     /locus_tag="P5549_05085"
     CDS             complement(23354..23683)
                     /locus_tag="P5549_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRW12478.1"
                     /translation="MVHTIVRFVVSAIVLLLISYLLPGIRVSGFWGALIAAVVIAIIG
                     YLIEMALGENVSPRSRGIVGFVVAAAVIYLAQYIIPNYLSVSLLGSLLAAFVIGLVDA
                     FIPTELR"
     gene            23788..24687
                     /locus_tag="P5549_05090"
     CDS             23788..24687
                     /locus_tag="P5549_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12479.1"
                     /translation="MRLVKINGNSYYIPAPANIGVFQFKDKYALLVDCGDNKQQARRI
                     DEIIKEQKLSVKYIVNTHNHIDHCGGNGYFQERSPGLEILTSATEKQFIEDNHLFSNL
                     LYGGQAPHELRRHFSKNPALTVTDTLVPGTCRINDEKFEIHSLPGHSPGQIGLATRDR
                     VCYLGDALFSAEKLRKYSLPFLFDIAAQLETYQTLSRLEYDHFVLGHAETVYDQPQLL
                     QLVDLNRSLLLESIELAREILSQPKTREELLEEISILRDLPLDFKEYYFSLSTVSAII
                     TYLYDQKQLVYQVENGKLYYYTG"
     gene            complement(24694..25002)
                     /locus_tag="P5549_05095"
     CDS             complement(24694..25002)
                     /locus_tag="P5549_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12480.1"
                     /translation="MMGPIIVLSHVLQALGKGTAAMWLSLFRQLGIFLPALMILPHFL
                     GLQGVWLAFSLTELLSALLAVVFFVNLWKQLQERRRYTFLMLRRRGYFKERFLSWLKW
                     "
     gene            complement(25061..>25240)
                     /locus_tag="P5549_05100"
     CDS             complement(25061..>25240)
                     /locus_tag="P5549_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12481.1"
                     /translation="AEADNIAWHGLIIAVIYGVFFSWLGLVNLERLLLGFGCSPESLA
                     DTASSRPGTDHPLCS"
BASE COUNT         6154 a         6877 c         5681 g         6528 t
ORIGIN      
        1 ttttttccta cttttacttc gctcattata tccctcccct catatgacca ggctatacaa
       61 gcacctaaat attgtacaat ctaatcatgt ttaaagtcaa gattattcag ggggccaacc
      121 catttctttt ccgcccagca aatggtagtg gagatgaaaa attgcttgtc ctcccccgac
      181 accgcaattg tttaccagac gaaagccctg ttcggcaata cccagcgatt cagccagctg
      241 agcagccagc aactgcaatc tgcccaggat ttctatctgt tgctctccaa catgatttaa
      301 ggaggtgata tgcattttgg gcataagcag aagatggacc ggggccgcgg gttctatatc
      361 cttgattgca atcaagtaat catcctgata gacgatttcc gacgggattt ccccgttgat
      421 aatcctgcag aacaagcaat ctgtttggct cacagctttc acctcctcaa tgctggatag
      481 cgttcttgac aataaatccg tataacctat tttatcagaa tttctaataa tactaaatgt
      541 atttttacat cccggagcga ctgtctttct gcctggcaaa aaccctacag ctgctgggat
      601 acgacctgct tgtgcaggtg ttcgctgccg gcgatggctg acaacatttg ggcgtcacgc
      661 agatattttt ccgccggatg gccgacggta aatcccatcc ggccgatgat ctccaccgcc
      721 tggaaggtcg tccactgggc tacacgcgtg gcgtagattt tggacatgta tgcggcccgg
      781 gaatagttgt cgtctgtatc aacataatgg gcggcgcgca ttacggccag tctggcagca
      841 tcaatttcgg ttgacatatc caccagggta gaactgacat attggttcac ggtggagttg
      901 cggttcaaca tctggttggt gcgcaccacc tgcaaggtta attcgtacgc agcccgggca
      961 atcccgacac caatcgcagc caccaaaggc atgttgcggt caaaaatctg ggtgaacaga
     1021 aaatacccgc tgccatcccg tccaattctg tattccagcg gaatacggac atcccgaaag
     1081 gccaactgca ccgtgtgact ggaacgcatg cctactttat ttaaaacccg gttggtttcc
     1141 agacccggag tcctgcccgg caccagatac atttgcagat gcgacttttg tttggattcc
     1201 ttgctccttg ccaacacggc aacaaaatcc gaagcgcctc cgttcatgac aaagtctttg
     1261 atgccgttga gggtaatctg cccgtcctgc gcttcagctg tcgtggtaat ccgggtaata
     1321 tcctcgcggg gacggctgct gcgatccagg tcatgttcgg gtgtgatctc actaatcgcc
     1381 agacatgcca ggtgcggtcg tgacttgcac aaatcgggca gaaattgctc cttcaatgca
     1441 tcgctgccag caatcagcaa cggggtaagc gcatacatgt tcatcaccac aattgcagct
     1501 gcccctgcac agcccagggc aatttcctcg atgatggagg tagccgccat catgcttaat
     1561 tctctgcctc catattcttt gggcacaatg ggggccagca aattcatttt tgccagatac
     1621 tccggtatct tccaatctac cttgtcatcc ccgacgcgat ccaagtaagc tccgcgttca
     1681 gcgattttgc tgatggacag ctcatgcacc tcgcgaatga gctggttttc ttctttgctt
     1741 aaggtaaaag cagccattgc tttgctcctt cctgttcatc aatgatttat aactgggcag
     1801 ctatcagcga tttatggaac tgctcacttc catcaatgat tgaaagcatt tccgcatccc
     1861 gcaaataaat ctcagacgga tagcctttga cataagccct tcctccaaca atctccatgg
     1921 ctttgctgct gatatcatgc gcagctcggg tggatagaat cttggctttg gaggacgcct
     1981 gggaactgtc caattcctgg tcaatcagcc aactggccag atagacactt tgacgggcag
     2041 cattcaattg agctgccatg tcaaccaggg caaaactgat ggcggtttcc tcgaaactgg
     2101 gacggcccat aatataacgt ttcttcgcga tagccagcgc cgtttcatac gctgcccggg
     2161 caacgcccag cgacatggca ccgacatagg gtaccatacg atcaaggttt tgcataaaaa
     2221 tcaaaaagcc gctgcccggt ttgccgacaa tataccgggg ctcgactttc acattttcaa
     2281 aaataacttc gcatgaattg cagtatttta aacctatttt ttgtcggacc gcgccggtac
     2341 gaatacctgg caatgacagc ggaatgacta cgatctgcag tccggttttt ttctcaggtc
     2401 cgtccagggt aaccagaagg gtaataaaac ttgccacctg gccgtttaat acataatcct
     2461 tgaacccgtt caaaatcagg caaccttcct gccagaatcc ggttaccgag gagttgatga
     2521 tttctatctg ggcgttttcc accagtatat tcaggttggg acgattttcg gtcattgcca
     2581 gcgaacccag gcatgctttt ccgctggtca gcaggggtaa tacttcctgc cgctgggttt
     2641 cggttccgga agtcaccaac ggacttacgg catgcatgtt ggccacggcg gaggccgcaa
     2701 ctccggcaca ggctaccgca atttcctcca caatcatggc agcgcttaga tggcttaacc
     2761 cccggcctcc atattcaggc gatatactgg gggcaagcaa attgtgttcg gctaacagat
     2821 cgatggctga ccagtcgaaa ttttcatttc cctgggcatc atagacaacc gctttgggcg
     2881 caatttcctg acggaccaaa tcgcgcacca tcatcaccag gtcccgatcc tgagcggaga
     2941 tcgtacttct aaacatatca tgtcctccta gttgctacga gtcttttgcc agatgatata
     3001 atccattacg gaacgcctcc gctcccgcgg aagtgtataa agcgtattga taatctgcag
     3061 cagttcctga tcatcatctt ccgaactggc aatatgattg accgccggcg tttctgtatg
     3121 actggaggaa ctctccatga aatactcgat cggcttgtct aaaagctcag caatattttc
     3181 cagctgagta agatacaaac gacgtttgcc tttttcataa ttggacagcg ttgattgtgc
     3241 acatcccaac atgttggcca gctgttcctg gcttagaccg gcttcctccc gcgccagctg
     3301 aagttttttc ccaatctcct taaatcccat ggagcgaacc cctttcaatt gttgtagaat
     3361 aattgctctc aatcctgatt aaaataagta ttcagttatt gtggcggctc tatgcatata
     3421 tttcccctta atccagtgga atcagcagaa gcgatttcaa ctgccactga ctgcccgcac
     3481 aaatcatgat ctgattggac cgttacttcc aaataattat cagtcagtcc tatatattta
     3541 tctttacctg atttttcttc gacaagcaag tcaaatcgtt gccccagaaa gcatttggca
     3601 aatgccagct tcttgtttct cgctatttcg agcagttttt cgctgcgttg attttttttt
     3661 ggttcaggga tctgcccgga taattctgct gctttggttc ccttgcgccg cgaatattta
     3721 aaaacatgta aatccaccaa aggcaactcc ccgatcaatt cgagggtatc ctgaaaatcg
     3781 ctgtccgttt cttctggaaa tcctaccata acatcggttg taaaagccat cccgggaata
     3841 cgatcagccg ctgtccggat caacttccgg taatcatcac ggtgataacg tcgccccatg
     3901 gcttggagca ctttgttgct gccgctctgc aacgggatat gcaaatgtct gcacatgcgc
     3961 tgatcagctg cgacgaggga cagcaagctg tcatcgactt ccaatggttc gatggagctt
     4021 agccggatcc gatatgtacc ggcgatttcc tctaaaatgg cgttcaccag acgactgagg
     4081 gtccagcccg gcaaatctat tccataaaat ccggtatgga taccggtcaa cacgatttcc
     4141 ttgtaaccaa gacggatgag ctgttcaact tcctgcaaaa ccacagccgg ttgcttgctt
     4201 ctgaccggcc cccgggccat cggcacaata cagtagctgc aaaaactctg gcaaccgtcc
     4261 tggattttta caaaagcccg ggtgcgctga tgctggcaat ggtataaaat cttctggggc
     4321 tggtcttccc gccggatggg gttgacgatg atttgctgat cagatcggtg ctgccgcaaa
     4381 taatcttcaa tcagcgatgc cagattatgc ttgtcgcgat tgccaaccac cagtttaacg
     4441 ccttcaatgg cggccagttg ggcgggatca acctgggcga cacatccagt cgcaaccacc
     4501 agtgcacctg ggttacgacg cacggctctc ctgaccatgg cccgtgattt acggtcgctg
     4561 acatgggtca cagtacaact gttgatcaca tagatatcgg cgatctcatg aaaatccacc
     4621 acctcataac cacggtgact gaaatcctcg attaagcctt cggtatcaac ctggttgact
     4681 ttgcagccca aggtatggaa tgcgattttt ttcatgcttt atcctgcttc atcttgtttg
     4741 tcccgtttat cctgtccaat ttcaacccag atcagccagt tcatacaaca cgatggtcaa
     4801 ggcggccaat ccagccgttt cggtgcgcag tatgcgcggt cccagtccgg cagtcatcac
     4861 tccctgggaa cgggcctgtt ctacctcggt ttcagcaaaa ccgccttccg gaccaatgat
     4921 taaaaaaagc tcctgttccc ccaacaggta agggcacttc tccttcagca attccttgaa
     4981 cccttgttgc ttttcgtttt catagagcat aatcgctggt tgggcatcaa taatctccag
     5041 catggacgcc aaagaaagcg gcggatacac ctcgggaata cggttgcggc ggcactgctt
     5101 gcaagcctca cgggcaatca cccgccatct ctcctgtttt ttcagggctc gctctttctc
     5161 cagcttcacc accgcatgtt ggctgctcaa cgggtagatg gcatccacgc ccagttcaac
     5221 cgctttttga ataataaaat ccattttatc gcctttggcg ataccctgta ccagttttac
     5281 ccgcatgaca ggttcatcgc caaccggcag cctgcggatg atttccccct cagcctgccc
     5341 ggccaccacc cggcttaaaa caacttcata ttcccgccct aatccgtcaa aaaccataat
     5401 ccgatctcca ggaacaaagc gcaggacctt ttctatatgg cggctctgct cctgatccag
     5461 acggacttga ttccccttga tttcctgcgg atttacgtaa aaacgatgca tattggttgg
     5521 ctttcctccc gggaaaacgt ctattgcctg cgggcagcga tccccatcca ttcattctcc
     5581 tgtaataccc ggtcaatgat gaaaccatgg tcttccaact gttgccgaac ttgcggccag
     5641 cggctttcaa taatcccgga acaaaacaga tacgccccgg ccgacagcaa cctggctgcc
     5701 cggggaatca tcaacgatac tacttctgcg gtgatattgg caaagatata gtcggcttta
     5761 aatccggcca gatcttcggt aatatcacgt acttcaacgg caatgatgtc ttcctgacgg
     5821 ttcatttgca cattggcccg ggcaattttc accgccagtt catcaatatc catggccaat
     5881 acccgggcgg cccccagctt ggcagcggcg atggccagaa tcccagagcc acagccagcg
     5941 tcaatgattc tttcgccgcc ccggacatgc tcatcgatga aatgcagaca aaaccgggtg
     6001 gaggcatgac tgccggttcc aaaagccatg cccggatcaa gctcaattac catctcaccg
     6061 ggctggggcg aatattcctc ccaggagggt ttgaccacca aacggtcacc aactttaaag
     6121 caatggtaat attttttcca tgactcttcc cagtcttctt ccttgacttc attgaggagc
     6181 aactggcaaa cgacgtgaaa attttcttcc acccggtaca atgcttcctg cagttgggca
     6241 tctatatcgc gcgcttcagg aaaataggct ttgaccgtga ccaaatcatg tttcccatac
     6301 gcaggcaggg caggaatatc gctggttccg gcgatgattt gccctacatc agagtgagcg
     6361 ggaacaatgc tgaccccgcc cgaaccgagg ttgtgcaaaa tgccggctac ggcttccaca
     6421 cactggcctt ctgtaataac cgcatattct ttccagttca atcgatttct cctttatccc
     6481 atggcatcct ttaatttatt gataattcct ttttttccgt ctttattgtc atgatcatca
     6541 taaaaaccca tcagcaattc tttttgccgc ttggtgagtt tggtggggat ctttattttc
     6601 aagtagactt tcaaatcacc attgcgctgg ccttgcagat gtggaatccc tttccccttg
     6661 atggtgagca catcacccgg ctgactgccg gagggaattt ccagtttatg gctcatccct
     6721 cccagcaacg gaatttctat ttcagccccc aaagaggcct gtacaaaatc aatttccact
     6781 tccgtaataa gggtataccc ttcacgcgca aatttttcat ggggctggac cacgatcata
     6841 atataaaggt cgccggaagg acctccgttg ttccccagtt cgccttcgcc ctgcatgcgc
     6901 aaacgtgccc cggtatcgat gccggcgggg atttttacat tgatggtgcg tttctttttc
     6961 acttttccgg tccctttgca ggcggaacac ggtttttcaa tgattttgcc ttcaccctgg
     7021 cagcgactgc aggtcttgac cgtttcaaaa cggccaaaag gcgtgctttg ggcactgcgc
     7081 atctgacccg tgcctttgca ttggggacag gtttttatgg gagatcctgg ttctgctcca
     7141 ttacctttgc aggttccaca cttttcaacg cgatgaaggt cgaaatcttt ttcactgcca
     7201 aaaacggctt cctcgaaatc gatgtccagc cttacttccc gatcgctgcc gcgctgcgga
     7261 ccactgcgcc tgcgttgccc accgccgccg gccgttccaa acagcagatc gaaaatatcg
     7321 ctaaagccac ccatgccttc accgaatcca ccaaagcccc ctgcgccgcc ccgtgttgga
     7381 tcgaaagcat cgtgtccgaa acgatcatac gccgatcgct tttgttcatc gctcagcact
     7441 tcgtaggctt cggcaacttc tttaaatttg tctgccgcat ccttgtcatc cttgttgaca
     7501 tcgggatgat attttttagc caattggcga tacgcttttt ttagctgatc tggagtcgca
     7561 tcccgtgata cccccagcac ctcataataa tctcgtttat cggccattct tccccacctt
     7621 cctgcctgcc gaatactctt tctggcgcta ttcctgaaac agactaacac ggattgagcc
     7681 aaccgccgct tgcgctgtgg ttttatcgat gctcatccgt gttaaacctg attgatctgc
     7741 agctatgcaa ctgctctagt cttcatccgg aatattgtaa tcagcgtcaa acaccccatc
     7801 atcggccgta ttgccgggat tggctttttc atatgccttg gcggagacag catagatggc
     7861 attggtcagg gcttcggttt tggcagaaat atcatcaatg tcactgcctt ccaaagcttt
     7921 ttgcaaggct tccctggctt cttcgatgga acgcttatcc tcttcgtcaa cctgcccggc
     7981 aaagtccttc aatgtcttgt ccgcctgata aatcatgctg tcagccgcgt tgcgggtttc
     8041 aatttccttg aaacgttttt catcctcttc ggcatgcagt tccgcttctc ttaccatgcg
     8101 gtcgatttca tcgtcgttca gaccgctgtt ggcagtgatg gtgatgcgct gttccttgtt
     8161 ggtcgccaga tctttggctg ttacggatac gatgccatta acatcaatat caaatttaac
     8221 ctcaatttgc ggtacaccgc gtggtgacgg cggaatgcct gtcaactgga atctgcccag
     8281 ggtcacatta tgttcggcca tcttgcgttc accctgcagg acatggatgt ctactgaagg
     8341 ctgattgtca ctggccgtcg taaacacctg actcttctcc gtcggaatgg tggtattgcg
     8401 ttcgatgacc cgggtaaata cgccgcccag agtttcaatc cccagcgaca gcggagttac
     8461 gtccagcagt accacatcgg taatatcgcc ggtcaatacg ccgccttgga tggcagctcc
     8521 cagaccaacg acctcatccg ggttgatgcc tttgaaagga tccttgccca ggaatccctt
     8581 gaccgcatcc tgcaccgccg gtatccgggt cgaaccgccc accagaataa ttttatcaat
     8641 ttcagccggt tttaacccgg catccttcat ggcctgtttg agcggcccca tggtcttgtc
     8701 aaccagatca gcggtcatct cattaaattt ggcccgggtg agcgtccgtt ccaaatgcag
     8761 cggcccttcc tgggtagcag taatataagg gatgttgatt tccgtgctca tcataccaga
     8821 caactcatgc ttggcttttt cagctgcctc tttcaatctg tgcatggcca ttttgtcatt
     8881 gtttaaatcg ataccgttgg atgccttgaa ctcctgatta agccagttga tgatgcgatc
     8941 atcgaagtcg tctccgccca ggcggttgtt gccgctggtc gccttgactt caaaagttcc
     9001 gtcaccgatg tccaggatgg atacgtcaaa cgttccgccc cccaggtcaa agaccatgat
     9061 ggtctggttc tcttccttgt ccagtccata ggccagagcg gctgcggtag gttcattgat
     9121 gatccgcaat acttccagtc cggctatctt gccggcatcc ttggtggcct ggcgctgaga
     9181 atcggtaaaa taagccggta cggtaatgac tgcctgagtg attttttccc ccagataagc
     9241 ttctgcatcg gtttttagtt tttgcagaat catggcggat atttcctgcg gagaataggt
     9301 cttcccctct atgtttacct tgtaatcagt ccccattttt cttttaattg aagtaacggt
     9361 ccggtctgga ttagtgatgg cctgtctttt ggccacccgg cctgccagcc gctccccggt
     9421 tttggaaaag ccaaccaccg aaggggtggt tctttctccc tccgcattag cgataacgac
     9481 agcctcgccg ccttccataa tggctattac tgaattggtt gtacccaggt caattccaac
     9541 aactcttccc attttcaagg acacctccac aaattttctc aagtattttg gaaaccgctc
     9601 tacgctgttt ccggttctat gataacaacc atctccaacg ggtttggctg tgatctgact
     9661 atttgccgct gactttgacc agactgggtc ggatcacccg gtcatgcatg acataccctt
     9721 tgttcaattc ttccaggacg gtgttttccg gatgttcatc actgctgact accatcaggg
     9781 gctcatgata ctgggggtca aacggtttgc cgacgcactc aatggcctgc accccttcct
     9841 gacgaagcag atcaagcaaa ttgcgggtgg taatctccat tccttttttt agcccttcaa
     9901 aatcctgggc agcactggcg gcttccagtc cccgattcag gtcatcaatg atgggcagca
     9961 gctttttaac gatcgacaat tgtgcgaact tcacatattc ttccttgtcc cgttgactgc
    10021 gtttccttat attttcagct tcggccagac ttcttaaata ctggtcgtag tatttcccgg
    10081 cttcctcggt tttgctctgt aattgttcct gcagtatctc cagttcactt ttttcggaag
    10141 cgttattctc ttgcacaact tcctcgttgc cgttggcagc ttcactttgc agcgttattt
    10201 cctgcgcttc atatgaatta tcgttgttca tttcatcacc cctattccat ttcaagcagc
    10261 tttcctgtct caacttctgt catcttaata attgtattaa ttttcaggat cgccacagtt
    10321 acgtcagcag cggtcttaat ggcatgcatc ttcacccaag tcgggtcaac aattccagat
    10381 gccatggtgt cgatcatttc tccactgtcg cagtcgatcc ccgccgatgc tgaaccggtc
    10441 ttcatctgaa cggcaacgac ttcttccatc ttttccaaag gattaaagcc ggcattctgg
    10501 gcaatacaat gaaaaggttt gagcagtgct tcttttacac acaacacgcc ataggaactc
    10561 atgcctttta attcccaggc cagttcattc aaatgctggg ccgcccagat ttcgatcgaa
    10621 ccgccgccgg gaacaactcc tttcaggagg gcagcctgca cagcggcagc agcgtcgcgg
    10681 gcaatacgtt ccctttcctc aaccagttcc gctgtcgcag ctccgatcaa gatcgtggcc
    10741 atactctcac cactgccctg atatatcaag gtatgtccgg ctttttgatc gacctcaact
    10801 ttttcggcaa agccgagata cttttccaat tgttctgctt ctttggccag cgcgctgcgt
    10861 tttattttac gagccccact gcttctggcg atcttctcca tttcgacacg agaaacccta
    10921 tgtatggcaa tgatcccggc gtcgctgaag atctgttcgg cgatatcatc aatgctgcgg
    10981 tcagtcaata ctaaattgat tcccagggca gcgattttat gtagattctg ttggtattgc
    11041 tcctgactgt gcagataata ttgaaatcca ttttcggtcc cccgggcttc gctgtcaatt
    11101 gcctccggcg ccaaggcatc attgagcagc aagatcttgg cttttttaag cgatgtcggc
    11161 atttcctggt ttaacggttc ccggttgatc agcaccccgg taaacacctg gctttccacc
    11221 gcttcccggg ctacgacggt atcacagaac ttgaattccg gagcaaacat tttgtcttcg
    11281 ccaacgattt tggcaccctc aatgaccagt cgggcaactt ccatatcacc gcgtccggca
    11341 atgagggcga catccaatag cttctcatct tccagggtca acatccttac attctgtgat
    11401 tcgatcactt ccaaacaacg ctcggttccg agggtaatgc cctgaatgac tttcgtcacc
    11461 ggcacgcctt tgagcacctg ctcggtcccc accgatatca tggcgcctgc cagcaaagta
    11521 gcggtagtgg tcccatcccc tacctcatcc tgctgggctt gggcggcgtt gatgatcatg
    11581 cgggcggcag gatggttgac ttccatcagc ttgaggatgg tcgccccgtc attgctgatc
    11641 accacgtcgc caaatttgtc gaccagcatg atatccaatc ctttgggtcc aatcgtccct
    11701 tcaatggcct gggatattac ccgggcggcg ttggcgttgc tcagcagcgt ttgaaatttt
    11761 tgatccactt caccgttcac cagatggttg tctttcatgc catactcctg tttaattgcg
    11821 ttttttcaac aaatcttcaa taaatgtccg tacggaatca agggttccag cggctttcca
    11881 gtattccatc ctgatcggtc cgatcaggcc cattttcccc atgtctccac tggtttggta
    11941 accggtaaat acgagactca tttcccgaat atcttctgac tgattttcct tgccgatacg
    12001 ataatcaacg ttctgggtaa tactttccgg caaaatatcg cgcaacagtt catcctcttc
    12061 gaggatggtg aggatgcgct ttaatttgta aagatccttg aactccggtt cattcaatat
    12121 attcagggca ccactgatca gtaccttttc ttcgccggaa ttttccagca gactatcaat
    12181 ggcatccagt gccttgtcga tcacccggcg tcgatttttc agatcatccc gtaatatctg
    12241 caaatgactg cgtttcattt ctgatagcct tttcccccgg aaaacctgat taaaaacatc
    12301 ggctaccgat tgcagatcac taacggtgat gcctttggga acatcaatct tgcggtgcag
    12361 caaaagtcca atgtcggtca caacaagaac caaggcctgc ccctcttgaa cggggaccag
    12421 gtgcagatat tgaaattgag taaacttaat ggaaggaacg ataataaagg aagtgtaatt
    12481 actaacctga gccagaaaat tgcctacgcc ttgggcgata tcgctccaat cacgaagatt
    12541 ttcatgtaat agcttatcca atacttccat ttcctcatca ctgaggcttt ctttttccat
    12601 catccagtct acataataac ggaaaccttt ttcagaagga atcctccccg cagaagtatg
    12661 gggctgctcc aaatagccca tttcctcaag atcagccatt tcattgcgta ccgtggcagc
    12721 gcttacattg agtccgtgtt ttctcacaat ggcgcgcgac ccaaccggtt cggctgtctc
    12781 cacataatct ttgatgattg attccagaat cagctttttt ctctcatcca ggttcattct
    12841 tctcaccacc tgctctgtta gcactcgcat tcattgagtg ctaattggtg tttattaaaa
    12901 aatatcatcc gccccaatct ttgtcaagtc gtatcgttcg cgaagccaac aatccttccg
    12961 cggattgcgg gcacagaagg cttctataac agttctgcca tgaattgatt ggacaggaaa
    13021 tagccgcggc ggctcaagta tagacgatcc tgctccatga ttaacaggcc atcccggcgg
    13081 aacttctcaa ggatagcatg atactcctgc gcaaaatcta taccgaaccg ttcgatgaat
    13141 tgttgtctgg agatgccttg cgtcaaacgc agtcccaaaa tgacggcatc tacaaagcgg
    13201 tcgtgctggt tcatgctttc caggatttca atatcaatca tgccggagtc cgactcgtct
    13261 ggcagtccgc tcacggtttc ccggtttttc catctttgcc cgtcgcggaa gctgactgct
    13321 cccgttccca gaccaagata ctctccggct tgccaatata taaggttgtg ccggcattcg
    13381 ctgcccggct tggcaaaatt ggaaatctca taatgatgaa agccttgtcc gatcagggtg
    13441 tccaccgttg catcatacat gtcggcttgc agatcttccc cggtctctgc gtaccttccg
    13501 gcactgattt gtctggccag cgggacaaaa ggttcgagtt gcagcagata ggcagaaata
    13561 tgccggggct gacaattcac ggccgactgt aaattatact gccagtcgtc cagattctgg
    13621 ccggggatgg cgtaaatcaa atccagattg aaattgtcaa acccagacgc aacaatatcc
    13681 tccaccgcct gccgggcttc ccggacactg tggattcttc ccagtatttt cagttcttca
    13741 tcgcgaaagc tctgtacgcc cagggagatg cgattgaggc cggcactttt caaggcctgc
    13801 agtcggctgg catcaagggt ggcgggattg gcttccaggg agacctccgc ctgcggcaac
    13861 aaggatagat gctgccgcaa accctgcagt atcctgtgga tttgttccgg ctcgagcaga
    13921 ctgggggttc cgccgccaaa aaaaacggtg ctgaagcagc ggtttgccca ttggccagcc
    13981 tgttgctcga tttcccgcag cagcttgctg gtatatcgtt ccaaatcgtc cttgtcgggc
    14041 aacggccggg aataaaaatc gcaatagctg catttcctga cgcaaaaagg cacatggatg
    14101 taaatccctg cctggttgtt atttgtcact ttcaatgtcc atcactgaca tgaaagcatc
    14161 ccgtggcact tccactcgtc cgatctgctt catccgtttc ttgccctctt tttgtttttc
    14221 cagcaacttc tttttgcggg tgatgtcgcc gccatagcat ttggccagta cgtccttgcg
    14281 catggcctta actgattctc tggcaatgac ccggttgccg atggaggcct gaatggcaac
    14341 ctcgtacatc tggcgcggaa taattttgcg caatttttcc accagggcac ggccccggta
    14401 ataggctttt tccttgtggg caatggtgct gagtgcatca acggcttcgc cattcaggag
    14461 aatatccatt ttgatcagat ctgacggctg gtagccatgc aactcgtaat ccagggaggc
    14521 atagcccttg gtcctggatt tgagtccatc aaagaaatcg aacaaaatct cggccagcgg
    14581 gagataatag ctgatcagca cccgatgtgg cgtgatgtat tcaatattga taaatgtgcc
    14641 tcgtttttcc tgacacagct ccataattgt tccgacatat tcattgggaa ccatgatgga
    14701 agccttgaca aaaggttcca ttacatgttc aattctctgc gcatcaggcc agcgggaggg
    14761 gttttgcacg tacacttcgc tgccgtctgt cagctgaatc cgatagataa cgctgggcgc
    14821 agtagccagc agggtgagat catattcccg ttccagtctt tcccggatga tttccatatg
    14881 cagcagcccc agaaacccgc aacgaaaacc aaaacccagg gcatctgatg attccggttc
    14941 gtaaagcagg gacgcatcat ttaatttcag tttttccaag gcgtctctca acttgtcaaa
    15001 ttcactgttt tccagcggat acagtccgca aaacaccacc ggtttgactt ccttgtaacc
    15061 ggacaggggc ttggcggcag gctggttggc gttggtgatg gtgtcgccca ccttggtatc
    15121 agctacgttt ttgatggcgg ctgccatgta gccgacttcg ccagcctgca aaacctcaac
    15181 ctgggtcatg tacggagaga taacgccgat ttcgatgact tcatactcct tgccggagga
    15241 catcatgcgg atcatatcac ctttgcgcaa ttccccgttg gcaagccgga tataggaaat
    15301 ggctccttta taggcatcaa aataagaatc aaaaaccaac gcctgcaagg gtgccttttc
    15361 atctccccgc ggagccggaa tttgagctac aatcgcttcc agtacatctt cgatcccggt
    15421 tcccagtttg gcggaaatgg gaattatttc atccttgtca attcccaaaa cgttttccat
    15481 ttcctgtttg accatatccg gttctgcgcc cggcaaatca atcttgttga ccaccccgat
    15541 gatttccagg ttgagatcca tggccatata aacgttggcc agggtctggg cttcgattcc
    15601 ctgggaagca tccacgacca gcaacgcccc ttcgcaggcc gccagactgc gggacacctc
    15661 ataggaaaaa tcaacgtgac caggggtgtc aataaggttt aaaatatagg tcttcccatt
    15721 ccgggcccgg taattaagcc gcaccggctg aagcttgatg gtgattccct tttctcgttc
    15781 caggtccatc ttgtccagaa attgctcacg catttcccga tccgataggg caccggtgaa
    15841 ttgcatcagt cgatccgcca aggtggattt gccgtgatct atatgcgcaa taatgctgaa
    15901 atttcttatt atatcctgca atctaacccc tccaggctgc tcttgattgg taatcaacta
    15961 cccggctgta tcaaacaatt gtcattcatt ataacgcaaa accacccggt gcttcaatct
    16021 tatcctttgt cgtcaagtca tggcaactgg ttcagacaat cgttcctccc ccgaccacca
    16081 cgtcgccctc ataaaaaaca atggcctggc cgggtgttac tgccctgacc gcttctccaa
    16141 actccacctg cacgctgccg tcgcggcggg ggcgaatcat cgcccgggtc ggctgagcct
    16201 tgtaacgaat ctgcgcctcg acttccagcg gttgggtcag gttttccact gcaatccaat
    16261 tcaattgcac tacgctgcaa ccgtttgcaa aaacttcctc attgcgtccc acaaccactg
    16321 cattgcgccg ggaatcaagg gccaacacat aaaccggata accctgggcc aagcgcagac
    16381 cgcgccgctg tccgacggta taatgaagga ggcctcgatg ctgccccagc acttccccct
    16441 gccgattaag aatcggtccg ggacgggcgc tgcccggata acgatcttct accaggcggg
    16501 cataatcatc atcggtcaca aaacagattt cctgactctc gcttttttca gccacggtca
    16561 attccattgc cccggctatg gttctaatat cagctttgct gtatcccccc aaaggaaaca
    16621 gactgtgggc cagttgctcc tgattcagta aatagagaaa ataactctgg tccttgcttc
    16681 gatcctgtcc acgatataaa agatagcgct gccgctgtct atcatattga atgcgggcat
    16741 aatgtccggt agccaagtaa tcaaagccca ttgattgtgc ctggcgcagc agatccccga
    16801 atttgattaa ctgattacac ctgacgcagg gattgggtgt cctgccctgc aagtattcct
    16861 cacaaaaata ggcaattatc tttttttcaa aggcttcccg atcattgagt acataatggg
    16921 ggattcccaa ttggtcagcc acccggcggg catcttccac cacggagccg tcacgtcctc
    16981 cagcaggatt atcctccccc gggtcggaca agagctccag ggtcgctccc accacttggt
    17041 aaccggattg gatcagcagc aaagccgcta cggaactgtc aaccccgccg ctcatcgctg
    17101 ccatcacttt gcccgcgccc atatttatcc ccctcatctc tggttggcac cccatccggg
    17161 aattctgtgc ctgaccatct tcagaaccag ttcggtttct tccattttaa agccatagat
    17221 aatcagcccg tatatcgcag ctccgagcga tacacctgct gtcacagcca gcagctgatt
    17281 cattttgtca gcccagggca accagggcag cagcaactgg atactatagt ttaccacaaa
    17341 atacatggcg gttgacccca ggagcgccac ggtcagactg cgcatcagca ccctgccccc
    17401 aattgggccg gctttcaatc gcaaggcagc cgccaaaacc aggcaattaa aaatgccggc
    17461 cagagaaatc gccagcgcca acccttttac tcccataagg cctgacaaat acagcgaaag
    17521 tacaacattt aaaacaatgg ttgcaccagc agccatgagc ggagtcaggg tatccttcat
    17581 ggcgtagaaa cttcggttta aaaccattat tcccgcgtaa gcagccaatc ctgcacaaaa
    17641 gtataataaa acctgaaaag tcgtgtgcgc catggccgct gtaaactgac cctgctggaa
    17701 caaaagcttg attaacggtt caccgatcgc tgccagacca aacgccgagg gaattgtaac
    17761 caggaagatg gcgcgcaggc ccagcgaact gtgacgacga aaaccagcca tgtcaccccg
    17821 ggcagtcatt gcagtcaagg taggaaaaac tgctgtagcg atggctgcgc cgaaaatacc
    17881 gaccggcagg ttcatgattt tctgcgcaag attcaaggcg ctgatcatgc ccgcaccaac
    17941 cgacgaagcc agattctggg tcacaaaaag attcagctgg gcaacactta accccaacag
    18001 gactgggatc attaaaatca tgatcttcac aaaccccgga tttctacaat taaaagtaaa
    18061 atgataatga acccccaccc ggcgcaaggc cggaatctgc accgcaaaat taaaaaccgc
    18121 gccgaccacc acacctattg caaaagcacg gatacccaac tgctgaaaca aagcaattcc
    18181 caatataata atgaaaagat tgtataaaac actgcccaac gccggggcgg caaattgatt
    18241 gtaggaattc aaaatgccct gcgagatgcc gctcaacgcc ataaaaaaag tctggataaa
    18301 catgatatgg gtaagactaa ccgccatcgc agcataggcc ggcggaagtc cgggagccaa
    18361 cagtttgatc agcggcgaag taaagaaata agctgcggca atcaacacca gcagcagcaa
    18421 gacgatataa ttaaaaacta cactgaccgc ttcccatccc tcttcctccc ggccgttgct
    18481 gagataagtt gtgaataccg gaatcagtgc ggcgctaagc gctcctccta ccaacagcaa
    18541 ataaataaaa tccgggatgg aaaaagcagc ccgatacgca tccgtcagat aattctggcc
    18601 aaacagggtc atcataaccg cttcccgggc aaaccccaaa actcttgcta cagcggtcat
    18661 gagcatcatg aaaagggcgg ctcgggcgat ggtagcgccg gcatttgtca atcttttcaa
    18721 ctcccttggc gacggctgtt gtcatgagcc attgtgagat gaacaatatt ataacggtat
    18781 acgtaaagaa atgaaaaaaa tgacaggagc ccggacccct gtcatcggca gtttattgta
    18841 aaaacaaaac gtcgaaaatg gtaacgatct tctgcagata atcaacgcag gaacagtatt
    18901 gctgtctgac atcccgcagc acggtctgcc cggttttact gatgccctcc ggtgtcaggt
    18961 gatattcttc tcccaaagca ataatgttga tctgctgttt ttcgatctgt aaacccagca
    19021 ccggtttacg ctgctccgcg gtaaggctgc tgatcccttc actgctggta ttcagaccgg
    19081 caatgaccag accaaggaca atgataatca gcagggaact gaagatttta ttcattgagg
    19141 ctggatttcc tccttcaaga cctgaatgat gacatcggca aacaattcag ccgcataatt
    19201 ggcttcctcc aaagtattat aatcgctgcc caattccaaa agcaaggccc gggaatgaaa
    19261 ttcctggttg tatgtcccgg cttttgtggt aaccccttta atcaaaccag gatacatctt
    19321 ttccccctgg gcttgtagag cctgggcaaa agccagattt ttcttccatt caggatgagg
    19381 tttgcgttca tccgttccca cgatgatgag aacgcgggca cttttacgtc cgtctgctgt
    19441 aatggtaggc gctttggttg tacccggcaa actgtcgcga tgaacatcga acaacgccag
    19501 gatctcctta tccgtctgca gaatctgctt gaccgtcttg cgtgaattgg tataactctg
    19561 gtcataatca ggataatcat gaatcgtatt gacaaattca gccggcaagc cctgctgcac
    19621 gagcgctttt tgcaaattct cagccacctt gtttatcaaa ccacgttttc cgtccagacg
    19681 ggccttgccg ctgtcgggta tgtagctttc cgcactgtgg gtacagtaaa tcaatacttt
    19741 tttgcctcga aacaaccctt cggcgacagt tttttcttcc tgctccctgg aaggaactgc
    19801 ctcctggtca gaggaaacgt cctgcggcaa agccggttca acgactggcg cagccaccgg
    19861 tgtggcatag gccagtactt gcatatgtga atcgatcatg ttttctgcaa tactggcggt
    19921 ctgcatttgc tctgctcccc aatcgacaaa atgttccgtt cccagcatcg acaaacaggt
    19981 ttcccccaac aaccattcgg cctgatgctg gttgatggct atcccggaaa aaggcacact
    20041 gactttgatg atccgcagcc ggggcagaaa atccacaaat ccgcggtgaa aatccaccgt
    20101 cagtcctgcc gccacagaca gtataaaaag ttgaatcagg agtaaccctt ttaaaaaaga
    20161 ttttaaatgc ttttgtctca tccctgtcca ccccattgag ccttcactgc tatatactat
    20221 ggcaggctgg ccaggcatag aaccgcttgc atctcaattg tccaccgttc ttgccgggaa
    20281 acccttttca tgatatagtt tttcccgtgg gcaagaggct atccatattt gcctcagact
    20341 tttattgcaa tttaaataac atagtgatat agtagttctg ttagctttac atatccaagg
    20401 aggtgaaatg gtggccaaaa gtaaaacacc tgctaaaaga gcccgtatag cagaagacaa
    20461 tcgactcaga aacaaagccc agaagagcaa gctgaaaacc agcatcaagc attatgaagc
    20521 ttcattgact ggggataatg ttgacgcagc cagagaagac ttaatcaaag taacctctct
    20581 gatagataaa agcgttacca aaggtgttct gcataaaaac accgctgccc gcaagaaatc
    20641 agctttagcc aaaaaattca acagcctgac cggcaattag catcaaaaaa acctgtctac
    20701 agacaggttt tcattttttc tgctgctttt atggcgcggt gcgctgacgc actgttatcg
    20761 ctggcagaag tcggtaatca ggatctccat tagcaaacga ggatccttgc tctggttttt
    20821 catactgata tctacctcca gcagcttgtt gaacagggct tgaatatcct gggcagaaaa
    20881 acggctgctt ttttcctgca ttttacggat gacaaaatct ttctgtttgc tcatttctgc
    20941 gatctgattg aatgaatagc cagcttcctg ccagaacttg atctttgcca aggtcaccag
    21001 ctgtccggtg atcatatgca gcatgagcaa atagggctga ccttgttcct gcaattgttg
    21061 aaaagcctcc agggaagcag ccgtgttccg gtttagaagt gcgtcagtga gtttgaaaat
    21121 acttgtttcc tggcggctat ccagttcatc gttcagatcg gagactttga tactgccgtt
    21181 tttaacgatc aacgaaactt tttcgatcag gttctccaaa taatacatat cctgtccgct
    21241 ggaaaccaac agattcaagg cagccggttc tacggtcaaa cctagctggt tgcacttttc
    21301 cttgatccaa tcccccaaag ccttggcacc gggcgcttta atgttttccc cttgggcctg
    21361 tctgctcaat atcttggcaa ccgggttggc agcaacatgc tcggcggcac agataaccaa
    21421 cgtctgtcca acggggttcg agaccaaata gtcctccagc agatgcgcaa tttcttctgc
    21481 ttttttggct ttacggctat tttttcccaa ccaagcagga ttcttgatga tcaccacccg
    21541 cgcgaacgaa aacaagctgc tggcttgcag ggtttctccc aattcacggg aactcatttc
    21601 atccccgtcc aatcgcacaa cttccggttc ttcaccgttc tctttctgaa tgcgggcaag
    21661 cagatcctta atcttttgct cgatcaggta aggctcgtca ccccaaaagt aatatattcc
    21721 tcgtgcttgt gccatcgttt actgctccct gtatatttta ctgcctgtta tggttattat
    21781 atcctatgcc agtatccatc tgaaggtaaa gaccgacaaa tttatgaata tggtataatc
    21841 tatccggata ggtttcatag actgtaataa tattcgtctg ttgcagacgt ttgatgaagg
    21901 aggcgctatg ctatgaccga ccgttatgac gtaattatta ttggagcagg tcccgcagga
    21961 atctttgccg cccgggagct ttgccgcaac agttccgcca aggtgctgct cctggaaaag
    22021 ggacgtttgg tggacaaacg cagctgtccg gtcagcgaag gcgcagataa atgtatccat
    22081 tgccaaccct gttcaatcat gagcggttgg ggcggcgccg gtgctttttc tgacggcaaa
    22141 ctcaccctga ccaccgaatt cgggggttgg ctcagtgatt atatcggcaa acaacgtttg
    22201 gccgaattga tcgaatatgt cgaccaggtc tatctccaac atggcgcatc ggacaccgtc
    22261 tacggacaaa atgaagaaga attcgaaacc atgcgtctgc acatggcgcg tgccaactta
    22321 aaactcctgc ccgcgcggat cagacatatg ggcaccgaca acactctgaa ggtactgcaa
    22381 aatttaaaaa ccgaactgga tccgcatatc accattaaaa ccggggaagc cgtggaaagt
    22441 attttggtgg aaagacatcg ggcgaaaggg gtacggacca aacgcggcag ctattatgcc
    22501 ccccgcatca tctgcgcgcc tggccgcgat ggggcgcaat ggttttccga gcagatgttg
    22561 aaactgaaaa ttccgctcag caacaaccag gttgatatcg gtgtcagagt tgaactgccg
    22621 gcggttacca tgcaggattt taccgataaa gtctatgaac cgaaaatcgt ctacaatacc
    22681 aaggaatttg atgatttggt cagaactttc tgcgttaatc ccaatggaca cgtggtcatg
    22741 gaaaacacca atggaatcat caccgtcaat ggtcattctt atgcaggaag caaaagcaac
    22801 aacagcaatt tcgcgctgct tctaagccag gaatttaccg agcctttcaa tcagccgatc
    22861 atttatggac gttgtattgc cagtttggcc aacatgctga gtggggggat tatcgtgcag
    22921 cgttttggag atttgaaacg cggccgacgt tccactaaag cccgtatcgc caaaggcctc
    22981 gtcgaaccca cccttaaggc ggcagtaccc ggcgatttgt cgctggtatt gccccatcgt
    23041 tatatggtcg gcatcaaaga atgtctggaa gcgcttgaca aagcaatccc cggtctggga
    23101 gcagatcata cccttctgta cggcattgaa accaagtttt attcagcccg tccgcaatta
    23161 agcagcagcc taaaaacctg tattgaaggg ttgtgggcta gtggcgacgg ggccggcgtg
    23221 actcgcggat taattcaagc cagcgtatcg ggagttgttt ccgcccggga aatcctttca
    23281 gaaatataaa ccaagcccct gccgctatcc agtggcaggg gcaaatcccg gcaacgatac
    23341 actttttgcc atgttacctt agttcggtag ggataaaggc atctaccaga ccgattacga
    23401 aagctgccag caaacttccc agcaaagata cggacaaata attgggaatg atatactgcg
    23461 ccaggtaaat gaccgcggct gcgacaacaa atccaacaat accgcggctg cgcggggata
    23521 cgttctcgcc cagagccatt tctatcaggt agcctatgat ggcgataacg acggcagcta
    23581 tcaatgcccc ccaaaaacct gatactctga tgccgggcag cagataactg atcagcagca
    23641 gcacaatagc cgagacaaca aaccgaacga tggtgtgaac catagagaaa cctcctttta
    23701 ttgatgctag cattcattgg ctatagcata gttttaccca agtaggaacg gaaacataca
    23761 tgcaactgca atgtcgggag gcaaatcatg cgactggtaa aaatcaatgg caacagttat
    23821 tatattcctg ccccggccaa tatcggggtt tttcaattta aagacaaata tgccctgctg
    23881 gtggattgcg gcgacaacaa acaacaagcc agacggatcg acgaaatcat aaaagaacaa
    23941 aagctttccg taaaatatat cgtcaacacc cataatcata tcgaccattg cggtggaaac
    24001 ggatattttc aggagcggtc acccggactg gaaattctca cttcagccac cgaaaaacaa
    24061 ttcattgaag ataaccacct gttcagcaac ctcctgtacg gcggacaggc accgcatgaa
    24121 ctgcgccgcc atttcagcaa aaatcctgcc ctcacggtaa ctgacacgct tgttcccgga
    24181 acctgcagaa taaatgacga aaaatttgaa attcattccc tgccggggca cagccccggt
    24241 caaatcggcc tggctacccg cgatcgggta tgttatctgg gcgatgccct cttcagtgca
    24301 gagaaattgc gcaaatacag tctgccgttt ttgtttgaca tcgccgccca actggagacc
    24361 taccaaaccc tgtctcggtt ggaatacgac cattttgtac tggggcatgc ggaaactgtc
    24421 tatgaccaac cacagttgct gcagttggtt gacctcaacc gctcccttct gctggaatct
    24481 attgaactgg cccgggaaat tttaagtcag cccaagaccc gggaagaatt gctggaagaa
    24541 atcagtattt taagagatct gccgttggac tttaaggaat attatttctc cctctcgacc
    24601 gtcagcgcga tcataaccta tttatatgat caaaaacagc tggtttatca ggtggaaaac
    24661 ggaaagcttt attattacac cgggtaaccc ggttcaccat ttcagccagg aaagaaagcg
    24721 ttctttaaaa tatccccggc gccgcagcat taagaaagta taacgccggc gctcctgcaa
    24781 ttgtttccac aggttgacaa aaaagacgac ggccaacagg gccgacaata attccgtcag
    24841 ggaaaaggcc aaccataccc cttggagccc cagaaagtgg ggcagaatca tcaaagccgg
    24901 caggaagatg cccaattgtc tgaacagcga aagccacatg gcagcagtcc ctttgcccaa
    24961 agcctgcagc acatggctca gaacaataat cggtcccatc accggtaaaa aaagggtcgc
    25021 cagttgcagg caatcaacgc ccctatcgat cagaagcgga tcacgagcaa agagggtgat
    25081 cagtgcccgg gcgtgatgat gcagtatcag ccaggctttc cggactgcag ccgaagccca
    25141 gcaacaacct ctccaggttg accagtccca gccaggaaaa gaaaactccg taaatcacag
    25201 cgatgatcaa tccatgccag gcaatattgt cagcctcggc
//
LOCUS       DAOTHZ010000029        23859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2490_length_23859_cov_6.903210, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000029 DAOTHZ010000000
VERSION     DAOTHZ010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2490_length_23859_cov_6.903210"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1409)
                     /locus_tag="P5549_05105"
     CDS             complement(66..1409)
                     /locus_tag="P5549_05105"
                     /inference="COORDINATES: protein motif:HMM:NF025756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheC/YheD family protein"
                     /protein_id="HRW12482.1"
                     /translation="MQADAHIRLIGNARLPVDTLAFSEDCSSRLAKFAAENLVLYLGQ
                     SHAGFKLVTDPGLSAGTVKMNPTLMHSYGLHSNLSYAIAHQRKGIRLGPYIGISANFQ
                     PDKNKPFGKQSLFIRQLIEQAQSMGAICFAFRPQDVNFGQASIKGYTCQGYQWKKRLY
                     PLPDVIYPRCHSGITQQAMRQKLSNRGVRFLNPPGIGKWGTYKALRFNSRLHDYLPYT
                     CPINHPADLKAMLNKYSCVYLKPITGSQGKNIIRINHQPRGSGYEYQYRIQQRQIHGT
                     AASLEKLVQDLRCLLNNRRYLVQQQIDLLHLDGCIMDLRVMVQKNRSGSWIITGKVFR
                     IGEPGSITSNISGGGRVGKAQQMLEKFFAPTKVREILVQIDFLALETARTMETRLGPI
                     GELGIDIGVDKNAMVWLIEANLKPARSIFNLLEDYETRILSVRRPIEYAFFMAGF"
     gene            1551..2012
                     /locus_tag="P5549_05110"
     CDS             1551..2012
                     /locus_tag="P5549_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HRW12483.1"
                     /translation="MRVVSACLCGINCKYNGASNRCEEFVKEMRAGQVLPLCPEQLGG
                     LTTPRHACEIVGGTGADVLYGQARVINKAGEDVTVAFVKGAQECLTIMQMAGIKEAVL
                     QRRSPSCGVGKIYDGTFSGHLIPGDGVTAALFRKQGIKVWNDEEYLQHQGK"
     gene            2012..2998
                     /locus_tag="P5549_05115"
     CDS             2012..2998
                     /locus_tag="P5549_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HRW12484.1"
                     /translation="MNLRAISLFSGGLDSQLAVCVIKDQGIDVVGINFVTPFFGAEER
                     TMMAARDLGIDYHSFDIGPDYMEVLKNPVYGYGKNMNPCIDCHAYMFKMAARYLQEFG
                     ASFLISGEVLGQRPMSQTKSSLNAVSKLSGNAGLILRPLSAKLLPPTLPEKEGWVDRE
                     RLLDISGRSRTRQMQLAEHYAIKDYPSPAGGCLLTQENFARRLRLLLNINPAASAKDT
                     EVLKIGRHLYLDEDQLLVIGRNHNENVRLQELAREDDYLIKVYDRPGPLGLLRLMQGE
                     GTAQLSLAARIIARYSDARQENTANIKISRLSGDEKVLQVVPLPAEEVPELV"
     gene            complement(3001..3546)
                     /locus_tag="P5549_05120"
     CDS             complement(3001..3546)
                     /locus_tag="P5549_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRW12485.1"
                     /translation="MEGLKIVCLGDSITYGWPWGPEISWTTMLARVIDAEVINQGIPG
                     NTTSQMMERFDRAVLAHHPTHVIIMGGINDIVWQESFDRIVWNLRSMAEKAAEHDIKV
                     VFGMPTAVDDDYLEKLTQRIRNWINTHADQHGIPVINFHQAFYNQEGKILNQLLAADG
                     AHPTQEGYQAMFAQIDTSIFT"
     gene            complement(3560..4459)
                     /locus_tag="P5549_05125"
     CDS             complement(3560..4459)
                     /locus_tag="P5549_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12486.1"
                     /translation="MLQKITDQLYFLLSETGFTYCNCILVEDDVRAILDTGADNKSIQ
                     SIHPEKIDMVLNTHHHYDHTRGNQFFPDARLYIHALDYSPLSSRDDYVHFNSIDMWDT
                     LMPDHDYNAAANLMGIDEADVERNMRVDQVFNHNQVIDFGHTKMEVIHTPGHSAGHCS
                     FWFPNEEFLFTGDICLTQAGPWYGEVYANPDDMIKSIERLIELNPPRMTSCHINEICY
                     DCRHRLTEFRDRIYKREERIFKSLQRQPVDLHQLAGQKLIYRMHPTPFVLFWEKCMLL
                     KHLHSLQQKGLIENLENGLYRAL"
     gene            complement(4505..7279)
                     /locus_tag="P5549_05130"
     CDS             complement(4505..7279)
                     /locus_tag="P5549_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HRW12487.1"
                     /translation="MQNENKNNRNSMKLFILFLLIAAFSIFSSYYVDWLWFSSLHYEN
                     VFTVTLLSKILLYVGVFVIAFLFVWLNLQLTRKFRNKDEVTRPQIDPDNEVIFLHPES
                     SPWQRFLEGQTSRWTFLVIGLVVAYMVSASTTDQWIVVQQFLHRTTFGNVDPVFSKDI
                     GFYFFHLTFYQFVYRTLMLMLVMSTIVVAILYVIGSTSELFALDWKKFTTAKSHVVIL
                     LSAIFALKAWGYQLSAYNVLFSNQGVVFGATYTDIHARLIMYKVLMIIALLVALVILV
                     NLFIKKMNWIIYSITAWFVAAVLLGGIYPGLTQKFIVQPDEFNKEKPYLERAIAFTRA
                     AYELDTVDNKEFNIEYDLTMDDINDNRATVDNIRLWDWQPLKDTYKNLQELRPYYIFN
                     DVDIDRYTVDGRYRQVMLSAREMEDIYKSAEMSPQAKTWINQRLMYTHGYGVVMSPVT
                     EIAQEGFPKFYIQDIPPMSNVDIKITKPGIYFGEKTDTYVIVNGKQKEFDYPVGSENV
                     YTNYEGNNGIRINSFFRKLLLSWELKDYKIILATNITNQSQVLMNRSLSERVKKVAPY
                     LAYDGDPYIVINDDGKLYWMLDAYTYSSKYPYSQPFDDYGHNYIRNSVKITCDAYTGE
                     LSFYMADENDPIIKTYQQIFPQLYKSIAEMPAGLKSHIRYPEDLFSIQADIYRSFHMS
                     DPWVFYNKEDSWVVPQEIVEGEEQPISPYYIIMRLPGEEKEEYILMMPFSPNGRNNMI
                     SWMCARMDGDNYGKKLVYRFPKQETVYGPMQIESRINQNTEISSQLALWNQQGSSTYR
                     GNILVIPINNSILYIEPLYLQAKASKMPELKRIIAAYGDTVVMETSLENALVKVFGGE
                     FNKPDVTKPTTPTEEPDTSPSVDTSELAKMARQYYDQANEALKQGDWAGYGENIKKLD
                     EIINQLQTSI"
     gene            complement(7543..7848)
                     /locus_tag="P5549_05135"
     CDS             complement(7543..7848)
                     /locus_tag="P5549_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12488.1"
                     /translation="MTNFLPAGIINETISDINQKAREMKTLLADNRLDDVKKVLNELE
                     EIALDLWVFIERFQCEPLIYTGPGKTDEVIKRLEWALAFSEEDIEQLLKTLNNKKKA"
     gene            complement(7914..8855)
                     /locus_tag="P5549_05140"
     CDS             complement(7914..8855)
                     /locus_tag="P5549_05140"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRW12489.1"
                     /translation="MNPKRILVLMGGMSEERDVSLRSGKAIWEALLALGYEAQALDFN
                     RTAIRDIIDYKPDLVFIALHGKYGEDGTVQGLLESLQIPYTGSGVATSAICIDKVLSK
                     RIFTSEGIPNAAYQIINRPELDQPDVIREQLLTRIGLPLVVKAATQGSSIGTYIIKDE
                     KEIITAIEKAFQYSREVLVEAFIDGQELTVAVIGNNEPQVLPLIEITSANSFYDYESK
                     YTKGMCEHIIPARIGEDSAAKIVSISQQVYKLMGCQGFARIDFILDKQGQPFVLEVNT
                     IPGMTEMSLVPDAARAAGIEFNELVEIIVNMALANHS"
     gene            complement(9092..9167)
                     /locus_tag="P5549_05145"
     tRNA            complement(9092..9167)
                     /locus_tag="P5549_05145"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9132..9134),aa:Trp,seq:cca)
     gene            complement(9249..10262)
                     /locus_tag="P5549_05150"
     CDS             complement(9249..10262)
                     /locus_tag="P5549_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12490.1"
                     /translation="MTNKNLTRSGIMYFDSGFYVLRQIMPYPLGETNVFLTESKDGWS
                     VIDVGVDLESTRRTWEQAVREIGISFKQIKHIYITHCHPDHMGAARWLQECSDAPVYM
                     LRKEIDRARNFIFLEQANFKKLYRQTIMETISANQFPEDKLEELIHDWYAEVRPLYLE
                     PAEILPIDAGDTIDLGGNNFSILSAPGHTDGQAMFWSNQHKYLVVADVVTASGYLHFS
                     DWPNTWLNNPLADTLSLFEKIQALEVKKAFPGHGPIITDINLQIDKLITKHQKMLDRI
                     ETAVTNPMSAGELYPHLFRLTEYVHGHRVTLGETLGYLNYLVSQGRLNLKKDNGLNLY
                     MPV"
     gene            complement(10659..11741)
                     /locus_tag="P5549_05155"
     CDS             complement(10659..11741)
                     /locus_tag="P5549_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRW12491.1"
                     /translation="MEYLTTSYHYLMQNVPASWQAGIMALLIFIFFLVLGKFISTTII
                     SAVLKLVSRDSTNLNNRIIQSFQKPLRYLIIILGTYWALLYLPLTPVHDIFVGKLFRC
                     AIIIMLAWGFYELAGTHSTISDEIKSRFKLDDILLPFFSKAIRFVIIALAIVLCASEW
                     DYDINGFITGLGLGGLAFALAAKDALANIFGGIVIIMEKPFSIGDWVTTPSVEGTVED
                     ISFRSTRFRTFAQALVTVPNSTLANEAITNWSRMGKRRVSFYLGLNYLTSPDKIEIAV
                     DKITQLLKSESTIHGDNVLVSFENFQESSLDILVQYFTRTTVWAENLKVKEEINLKIM
                     RLLQTEGISMAFPSRSIYIENGESTN"
     gene            complement(11921..13969)
                     /gene="fusA"
                     /locus_tag="P5549_05160"
     CDS             complement(11921..13969)
                     /gene="fusA"
                     /locus_tag="P5549_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRW12492.1"
                     /translation="MKVYPTSSIRNIALVSHGGTGKTSLAEAMLYSTGVLKRLGKVDD
                     GTSVGDYLPEEVKKKFTISASLIPCEYKDCKINLVDTPGYADFYFEVAGAMRVVDSML
                     MLFSATAGVEVQTQVAWEDYPHIPKIAFINKMERENADFYKVLAQIKDTFSDNVVPIQ
                     LPIGAADTFKGFVDLIKMKACMIEPGSGKMTETDIPDDMMDDVEMYKLELIEAAAEAN
                     DDLLTKYLEGEEITNEEIKDGIKAAASNGSAVFVMCGSVMNNMGVTALMDFIAACSPT
                     PDYNTLVKGKDIENEPLSAQVFKTIADPYIGRLTMFRIFTGKLKADSVIYNANKEKDE
                     KITQLLVMTGKEQSSVPELNAGDIAAVAKLSVTSTADTLCTKAKPVILDAIDFPRPTL
                     SMAIEPKSKGDEDKLSGALQRLMEEDPTITVEKNTETRQTILTTMGDTHVDILIDKLA
                     RKFGVDVVSREMRIPYRETIRTTVEVEGKHKKQSGGHGQYGHVWIKFEPFDEGEFTFE
                     ENVFGGSVPRNFFPAVEKGLQEALEEGVLAGYPVTNLKATLYDGSYHSVDSSEMAFKM
                     AARIAFKNGVEKAKPVLLEPIVNVEIEIPDTYMGDIIGDLNSKRGRVLGMEPSGKKQV
                     VKAQVPLAEMARYTIDLKSITQGRGKFRMEVDHYEEVPSQDADKIIAKAKKEREEKEK
                     "
     gene            complement(14115..14912)
                     /gene="surE"
                     /locus_tag="P5549_05165"
     CDS             complement(14115..14912)
                     /gene="surE"
                     /locus_tag="P5549_05165"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRW12493.1"
                     /translation="MRILITNDDGIHARGIQILVQELSQIAELYLVAPDRERSGTGHS
                     ITVFDPIKVTKTTFPGVSQAWIVSGTPVDCVKIAIARLIEENIDLVISGINHGSNLGS
                     DVLYSGTVSAAAEAVIMGCPSLAISLDSDHYESDFSFAVHLTRQLVSALMHWGIEPKT
                     LININIPAVERSQIKGIRICKLGLRNYDNLFEERQDPRGNTYYWLGGGLRQEEQDPDS
                     DVYAVEHNYVSVTPINLDMTNYQLIHKYKSLAVSFQKRNFPDLEISQ"
     gene            complement(14909..15454)
                     /gene="lepB"
                     /locus_tag="P5549_05170"
     CDS             complement(14909..15454)
                     /gene="lepB"
                     /locus_tag="P5549_05170"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRW12494.1"
                     /translation="MNKEIKEFFKETLSIVIIAFILAMILRTFVIEGRIIPSESMLPT
                     LKVGDRVMVNKFIYRFKQPQRGEIIVFAPPDVLQSKDDYIKRVIGLPGEKVEIKNNRV
                     YIDDQPLVEPYLAEPVNYTFGPVTVPENSLLVLGDNRNHSFDSHMWNAWMSQDRIKGK
                     AFVIYWPIKEIKLFERGVSFE"
     gene            complement(15521..15706)
                     /locus_tag="P5549_05175"
     CDS             complement(15521..15706)
                     /locus_tag="P5549_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmA family protein"
                     /protein_id="HRW12495.1"
                     /translation="MEESQKDTGKLELIALKNFSAHNELVYVIDFLNKSLKDKNIMFG
                     LSKDKQAGQMTIKIYEF"
     gene            complement(15781..16344)
                     /locus_tag="P5549_05180"
     CDS             complement(15781..16344)
                     /locus_tag="P5549_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HRW12496.1"
                     /translation="MIRVIKTLVSDKRITYDGTQMQSHWIHNQFGLVGDAVVAFIGQV
                     KVDLDKMIDLIDVSRKESIYSPLMLNFLIEHFNPNLELAIYRQWLFMTLIKEELSQFE
                     VNVYRLGDDLYFNKAKLSVSVATVSNVSSLIHIGLNIETEGTPIKTVGLSELGISDIN
                     SFADTIMLKYRRELEHIHEARCKVRGV"
     gene            complement(16498..20088)
                     /gene="addA"
                     /locus_tag="P5549_05185"
     CDS             complement(16498..20088)
                     /gene="addA"
                     /locus_tag="P5549_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HRW12497.1"
                     /translation="MNNWTKEQLQAIDARDSNLLVTAAAGSGKTAVLVERIIRMICRD
                     GLSIENLLIVTFTNAAAGEMREKITAAIIKEIEAMPDHQDHLRYQLQMIGRSSICTLH
                     AFCTSIVRDNFHLLDIDPKFRIGDTAECSILRREAVEEILEEAYDNGSAHFLDLVERF
                     AGNRSDQAVEDLILRTHNFIQSQPEPLAWLTEKIRIFALTEEEFVESQWADNILRDMQ
                     SRINAADDLFAAAFALIDQHASLEGYRPALEDDLVQTGQLTHAARLGLPALTDKITAL
                     HFTRLGRVARDCDPNLQERAKGLRNEGKKILQELTRITQGKNIAQFMAELRQMSSAMQ
                     ALVELVADFNRRYQEKKAEKGMLDFNDLEHYALQVLSNPDCARQYREKYQYIFVDEYQ
                     DSNLVQETLLQSIKSEHNLFMVGDVKQSIYRFRLADPTLFIAKYNTFVSEPSALNRRI
                     DLGKNFRSRQEILDGINYLFKHIMSEPLGEIDYDESCYLYYGSNMEAQEDARIEVLLL
                     EKDPALLELDEDQEELSDIEIEARLAAQRIHQLVGLTIYDPREKCHRPLQYRDMVILM
                     RSTKNRADVYLDTLSAQGIPVYADVNNGYFENLEVEMFLNLLRIIDNKKQDIPLLSVL
                     RSPLAKFTLEDLIDIRRNSRAAYLYQAAEECMQNQSDDLSRRLTCFWEQISHWKNKAR
                     FMPIDELIWEIMLETDYYYYVSAMPGGLQRQANLRILLDRARQFQTSSLKGLYNFLRF
                     VDSLQTDRGGDMGNARLLSEKDNVVRIMSIHKSKGLEFPVVIMAGLGKQFNLSDTNAA
                     VLFHKDLGIGPMFVDPQARVKCNSLARIAIQGQLKTENLSEEMRILYVGCTRAINKLI
                     LLGSLKNLPALTQKWSSSLNLYGLSKANTFMDWIGPVLLRHPDGQILRDLLAEEFDPR
                     QIIRDDSSWDIKIASPAAFGPSLAEAPSQASTVLQELQNFKRETPSPEAALINHRLNW
                     DYPYRAAETIPSKRSVSSIKKLKNNNWPANLPDIPLLLPGPVFINPPDSTAALSGAEK
                     GTIMHLVMQYLDLTRVGSTTEIDCQLQEMIVQGLLVEDEMRAVQTEKIAHFFASSLGQ
                     RMLHSSQVYREVPFNLVCKAAQVLEDINAGDEELLVQGVIDLYFVEGDDLVLVDYKTD
                     YISAQNRAALIAQYTVQINMYRQALESILQKKVKESYLYLFHPEETIAIA"
     gene            complement(20081..23503)
                     /gene="addB"
                     /locus_tag="P5549_05190"
     CDS             complement(20081..23503)
                     /gene="addB"
                     /locus_tag="P5549_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HRW12498.1"
                     /translation="MTIRYIIGRAGRGTDRLAMMEIGRELNQDSDQRLYLLVPEQYTL
                     QAERNLIKELNLPGIMRAEVISFNRLLYRVLNETGSHRHVLINEQGRSMVIKKVIHNV
                     ASDLTVYQQACHQQGFAEEIAGILTGFKQYDLDPAALRERVNEIREDSLRLKMQDITL
                     IFTAYTDYMADHYLDMEDQLNLFIEKLDDSKLLTNCQIWIDKFDHFPPQDLRIIEKLM
                     LQAKETTICLIDDLHPGRDRDLFKMSKRCYKNIHALAVQHGLEEKIIDLAAESHRWLA
                     MPRTASLKHLEQELYAYPYRSFQAETDAIALFAAANPNSEVEHVAGSIIRLVQERGWR
                     WRDIAVLGNNMETYGPMIARVFNEYRIPFFLDAKSSIDNHPLIKMILASLAIVTRGYR
                     PEDILMLLKCGFTDLSEDQSEEMENYLLAYGIRGKLWREDFTRGQDEIGAEQLTALNI
                     LRRQLFMPLQTLEKALAGVQTGQEIGQALYHYLQVLQMEAKLQDWTEQLRGDGLFEYA
                     LQNAQIWNIVMQTLDQLVEILGDQKIRAQEFANLLETGFASISIGIIPTTVDQVVIGS
                     LHRSKSHAFKAVLIMGVNDDILPAGKASQMLLTENEIMLLQTCQIDLDWDQATQSLTE
                     QVTIYQAFNQAEAFLSLSYAIADQEGRALRPSVLINRMKSLFPKIIVSSDLVRDRQIE
                     LEQVVTSSGSYKYLIEKLRLYLDGEEICDFWWDVYAWYENNSAWDQRRHELLDALFYQ
                     NQITGQEPGSASKLYPSPFYSSVSRLEQFAACPFAHFVRYGLRPQERKEFAVRMPEVG
                     SLLHEGLAIFACELDKQGIDWRTLDHAACNELVDKIMTEQLTVHNNGIMMSSSRYRYL
                     ARRLQRITRRAVWTLTEHIQRGDFEPYLYEARFGSDGAFPPIKIELNQQQTFYLEGRI
                     DRIDILETGEHTYCKIIDYKSSDRQLSLSELYYGLNLQLFVYLLAVLQSNPTAHQADP
                     ASHKYRPAGLFYFKIDDPLINTTAMEPAEIEASIRKKLRMKGLVLKDVALVRAMDNAV
                     QGDSDILPVGIKNDGEFNSRSNVLTLEDFSLLLRYLQDLLQQLGTRMISGHICIEPVK
                     TAQGKACDYCLYHSICQFDKLFTANRYKLIKPEKDAAILEKIKQLRRNDTNE"
     gene            complement(23664..>23859)
                     /locus_tag="P5549_05195"
     CDS             complement(23664..>23859)
                     /locus_tag="P5549_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRW12499.1"
                     /translation="YAIVRCMPQIGVGNATTAIIVAQVLTALLIDNFGLFGMKKMSFH
                     YLDLLGVVLLAAGARILLLE"
BASE COUNT         6303 a         5575 c         5318 g         6663 t
ORIGIN      
        1 catattttga taaagccgat tggtaatgtt gatttgcatc aattttcacc gtcctttaaa
       61 ataggttaaa aacctgccat gaaaaaggca tattcgattg gtcggcgcac agatagaatt
      121 cttgtttcgt aatcctctaa aagattgaat atgctgcggg caggtttcag attggcctcg
      181 atgagccata ccatagcgtt tttatcgacg ccgatgtcga tgcccagttc cccaataggg
      241 ccaagtcggg tctccatggt acgggctgtt tccaatgcaa gaaaatcaat ctggacgagg
      301 atttcccgga cctttgttgg cgcgaaaaat ttttccagca tttgctgagc cttcccgacg
      361 cgcccaccgc cagaaatatt gctggtaatc gaaccgggtt cgccaatgcg aaaaaccttg
      421 cccgtgatta tccagctgcc gctccggttt ttttgcacca tgacccgcaa atccataatg
      481 cacccgtcca ggtgtaacag atcaatctgc tgctggacca aatagcggcg attgttcagc
      541 aggcagcgca aatcctgcac aagtttctcc agactggcag cagtgccatg aatctgacgc
      601 tgttgtattc gatattggta ttcatatccg gagcctcgag gctgatgatt gatgcgaatg
      661 atatttttac cctgggaacc ggtgatgggt tttaaataaa cgcaggaata cttgtttaac
      721 atggctttta agtcagccgg atgattgatt gggcaagtat acggcaagta atcgtgaagc
      781 cgggaattga accgcaatgc tttatacgtt ccccacttcc ctattcccgg aggatttaaa
      841 aagcgtactc cccggttgct tagtttttgc cgcatggcct gctgggttat gccgctatgg
      901 cagcggggat agataacatc aggaagagga tacaagcgct ttttccattg atatccctga
      961 caagtgtatc ccttgatact ggcttggccg aaattcacgt cctggggccg aaatgcaaaa
     1021 cagatggctc ccatggactg agcctgctca atcaactggc ggataaaaag agactgtttc
     1081 ccaaagggtt tgtttttatc tggctggaaa ttggccgaaa tgccgatata gggacccagg
     1141 cggataccct tgcgctgatg ggcaatggca taggacagat tgctgtggag cccataagag
     1201 tgcatgaggg tgggattcat tttcacagta ccggcagaca gaccgggatc ggtgaccaat
     1261 ttgaagccag catgtgattg accaaggtat agtaccaaat tttccgcggc gaatttagcc
     1321 agccgtgaag aacagtcttc ggaaaaagct agcgtatcaa cgggcagccg ggcgtttccg
     1381 atcaggcgta tatgcgcatc cgcttgcatt gcatatctcc atttgctttt ttcttaccct
     1441 acaatatgaa tgcggtccgc tctttgttca ccaaacgcac cattcttgct tgccatgact
     1501 tgaatttgac cggaataata tatactataa aaaagttggg agatgattat atgcgagtag
     1561 taagcgcatg tttgtgcgga attaattgca aatataatgg agccagcaat cgctgtgagg
     1621 aattcgtcaa agaaatgcgg gccgggcagg tattgcccct ttgtcctgaa caattgggag
     1681 gattgacgac cccgcgccat gcttgtgaaa tcgtaggtgg tactggtgcc gatgtactct
     1741 atgggcaagc gagagtgatc aataaagcgg gtgaagatgt aaccgttgct tttgtcaaag
     1801 gtgcccaaga atgcctgacg attatgcaaa tggcgggaat aaaggaagct gttttacaac
     1861 gtcgcagccc gtcctgtggg gttggcaaaa tatatgacgg taccttttcc gggcatttga
     1921 ttcccggtga cggggtgacc gctgccctat tcagaaaaca gggtattaaa gtctggaatg
     1981 atgaagaata tctccagcat caagggaagt gatgaatttg agagcaatca gtctgttttc
     2041 gggtggtctg gattcacagt tggcggtatg tgtcatcaaa gatcagggaa tcgatgtggt
     2101 gggcattaat tttgtcacgc cattctttgg ggcagaagaa cgaaccatga tggcggccag
     2161 ggatttggga atcgactatc attcttttga tatcgggccg gattacatgg aggttttaaa
     2221 gaatcctgtc tatggctacg ggaaaaacat gaatccctgt atcgattgtc atgcttatat
     2281 gtttaaaatg gctgcccggt atttgcagga atttggagcc agtttcctta tttccggaga
     2341 ggttctgggg cagcgtccca tgagccaaac caaatcctca ttgaatgcag tcagcaaact
     2401 atccggaaac gctggcctta tcttgcggcc tctttcggcc aaactgctcc ctcctactct
     2461 gcccgaaaaa gaaggctggg tggatcgtga acgtttgctc gatatcagcg gacgcagccg
     2521 caccaggcaa atgcaactgg cagagcatta cgcaataaaa gactatccca gcccggccgg
     2581 cggatgtctg ttgacccagg agaactttgc caggaggtta cgtctgcttc tgaacattaa
     2641 ccccgcagcc agtgccaagg atacagaagt attgaaaata ggacggcatt tgtatttgga
     2701 tgaagatcaa ctgctggtta ttggtcgaaa ccataatgaa aatgtaagac tgcaggaatt
     2761 ggcccgggaa gatgattatc tcatcaaagt ctatgaccgg cctggcccat tggggttgtt
     2821 gcggttaatg caaggggaag gcaccgccca gctgagcctg gcggctcgca tcatcgcacg
     2881 ttacagtgat gcccggcagg aaaacaccgc caatataaag atcagccggt tgtcggggga
     2941 tgagaaggtg ctgcaggttg ttccgctgcc ggcagaagaa gtgccggagc tggtataaag
     3001 tcatgtaaaa atgctcgtat cgatctgtgc aaacatcgct tgataccctt cctgggtagg
     3061 atgtgcccca tctgccgcca aaagttggtt cagaatcttt ccttcctggt tgtaaaacgc
     3121 ctgatgaaaa ttgatgaccg gtataccgtg ctggtcagca tgtgtattta tccaattgcg
     3181 gatacgttgg gtcagttttt cgagataatc atcgtcaact gcggtaggca tgccgaatac
     3241 caccttgata tcgtgttcgg cagctttctc tgccatggag cgcagattcc agacgatcct
     3301 gtcaaagctc tcctgccaga caatatcatt gatcccgccc ataatgatta catgagtggg
     3361 atgatgggca aggactgccc ggtcaaaccg ctccatcatc tgcgaggtcg tattgccggg
     3421 gatgccctgg ttaatcacct cggcgtcaat caccctggcc aacatggtgg tccaggagat
     3481 ttcgggtccc catggccatc catacgtaat gctgtcgccc aaacagacaa ttttcaaacc
     3541 ttccatcctg agatctccct tacaaggctc tatacaaacc attttccagg ttttcaatta
     3601 aacctttttg ctgcagactg tgcaagtgct tcagcagcat gcatttttcc cagaagagta
     3661 caaaaggcgt tggatgcatg cggtaaatca atttttgtcc ggccaattgg tgcaaatcga
     3721 ccggttgccg ctggagcgat ttaaagattc gctcctcgcg cttgtaaatc cggtcgcgga
     3781 attccgtcaa acgatgccgg caatcataac agatctcgtt gatgtgacag gaggtcatac
     3841 ggggaggatt tagctcgatc aaccgttcga tggatttgat catatcgtcc ggattggcat
     3901 atacctcgcc gtaccatggg cctgcctgag tcagacagat gtccccggta aacaggaatt
     3961 cctcattagg aaaccagaag gaacagtgtc cggcagagtg tccgggagta tgaatgacct
     4021 ccattttggt atgaccaaaa tcgatcacct ggttatgatt gaacacctga tcgacacgca
     4081 tgtttctttc cacatcggct tcatcaatgc ccatcaagtt tgccgctgcg ttataatcat
     4141 ggtcaggcat caaggtgtcc cacatgtcaa tggaattaaa atggacataa tcatcccggc
     4201 ttgacaaggg agagtaatcc agggcatgaa tatacagacg ggcgtcggga aaaaattgat
     4261 tcccgcgggt atggtcataa tgatgatgcg tatttaaaac catgtcgatt ttctcggggt
     4321 gtatggattg tattgattta ttgtccgccc cggtatccaa aatggctcga acatcatcct
     4381 cgaccaatat gcaattacaa taggtgaaac cggtttcact cagcaaaaaa tacaattggt
     4441 cggtgatttt ttgcagcata cggcgatccc cctctcctcc gaatcaaacc caaagcttca
     4501 acgcttagat tgatgtctgc agttggttga tgatttcatc cagctttttg atattttcac
     4561 cgtatccagc ccaatcgccc tgtttgagtg cttcattggc ctgatcataa tactgtcgtg
     4621 ccattttggc caattcactg gtgtcgactg aaggacttgt atcaggttct tctgtcggag
     4681 tggttggttt ggttacatca ggcttgttaa actctccgcc aaagactttt accagtgcat
     4741 tttccagact ggtttccatc accacggtat ccccataggc agcaataata cgtttcaatt
     4801 ccggcatttt gctggccttg gcctggagat acagcggttc tatatataaa atcgagttgt
     4861 tgatcgggat gaccagaata ttgccccgat aggtactgga accctgctgg ttccagagcg
     4921 ccaattgact tgaaatttcc gtattttgat tgatgcgcga ctcaatctgc atgggtccgt
     4981 acaccgtttc ttgtttgggg aagcggtaga ccagtttctt tccgtagtta tccccgtcca
     5041 tacgagcgca catccaagaa atcatattat tgcgtccatt aggagagaaa ggcatcatca
     5101 gaatgtattc ttccttttcc tcaccaggca gacgcatgat gatatagtag ggactgatgg
     5161 gctgctcttc accttccacg atttcttggg gaaccaccca actgtcttcc ttattgtaga
     5221 agacccaggg gtcgctcatg tgaaaactgc ggtagatatc agcttgaatg ctgaacagat
     5281 cttccggata gcgaatatgg gatttcaatc ctgccggcat ttcggcaatg gatttataca
     5341 attgcgggaa aatctgctga tatgtcttga tgatcggatc attctcatcc gccatataaa
     5401 aactcaactc gcctgtgtag gcatcacagg taattttgac agaattgcgg atataattgt
     5461 gaccgtaatc atcaaaaggc tgtgaataag gatacttgct ggaataggtg taggcatcca
     5521 gcatccagta aagtttgccg tcatcattaa tgacgatata aggatcgccg tcataggcca
     5581 aatacggtgc aacttttttc acccgttccg acaggctgcg gttcatcagt acctggcttt
     5641 ggttggtaat attggtcgcc aggatgattt tgtagtcttt taattcccag ctcagcaata
     5701 atttacggaa aaaggaattg atgcggatgc cattattgcc ctcataattg gtgtaaacgt
     5761 tttcagaccc taccgggtaa tcaaactctt tttgtttgcc attgacaatt acataagtat
     5821 cggttttttc accaaaataa atccccggtt tggttatttt aatatcaaca ttggacattg
     5881 gcggaatatc ctgtatataa aacttcggaa atccttcctg cgcgatttcc gtaaccgggc
     5941 tcatgacaac cccataaccg tgtgtataca tcagtctttg gttgatccac gttttcgcct
     6001 ggggactcat ttccgcactc ttgtaaatat cctccatttc acgggcggag agcatgacct
     6061 ggcgataacg tccgtcaacg gtataccggt cgatgtcaac atcattaaat atatagtacg
     6121 ggcgcagctc ctgcagattt ttatatgtgt ccttgagtgg ctgccagtcc catagcctga
     6181 tgttgtcaac ggtggctcga ttgtcattga tgtcatccat ggtcaggtca tactcaatat
     6241 taaattcctt gttgtccacg gtatccagtt cgtaggccgc ccgggtaaag gcaatcgccc
     6301 gctccaaata gggtttttcc ttattaaatt catccggttg gacgataaac ttctgggtca
     6361 gtccgggata gattcctccc agtaaaacgg ccgctacaaa ccaggcggta atactgtaga
     6421 taatccagtt cattttcttt ataaagagat tgaccaggat caccagtgcc accaacaacg
     6481 caatgatcat caacaccttg tacatgatca gacgggcgtg aatatcagta taggttgcac
     6541 caaaaacgac tccctggttg gaaaacaaga cattataagc agacaattga tagccccagg
     6601 cttttaaagc gaatatagca gaaaggagga taaccacatg gcttttagcg gtagtgaatt
     6661 ttttccagtc aagtgcaaat aactcggaag tggaaccaat tacataaaga atcgcgacaa
     6721 caatcgtact catgaccagc attagcatca gcgtacgata aacaaactga taaaaggtca
     6781 gatgaaagaa ataaaatcct atatccttgc taaaaaccgg gtcaacattg ccaaacgtgg
     6841 ttcgatgcag gaactgctgt acaacaatcc attgatccgt cgtggaagca ctgaccatat
     6901 aggcaacgac taatccaatc actagaaaag tccaacggga agtctggcct tccaaaaaac
     6961 gctgccaggg agatgattca ggatgaagaa aaatcacttc attatcagga tcaatttgtg
     7021 gacgcgtcac ttcatccttg tttctaaatt tgcgggttaa ttgcagattc agccagacaa
     7081 ataaaaatgc aatgacaaac acgccaacat agagcaatat tttgcttaaa agggttaccg
     7141 taaaaacatt ttcataatgg agcgaagaaa accagagcca gtccacataa taactgctga
     7201 aaatcgaaaa ggctgcgatt aataaaaaga ggatgaaaag cttcatggaa ttacggttgt
     7261 ttttgttttc gttttgcaca gaatccctcc taaatatatt gtagaaaagt ggaaacttac
     7321 cgaaaaacat gactaaacat aagtttagaa tataagaata attttatcgg tgcgaattac
     7381 gaccggaaat tgctttttga taaaatacta aacaaatatc taaatgagac agcatacgtt
     7441 tgtcaagggg gttcctcgga aaatgtaaaa atgtaaagat gcctcaaaaa ctatccaagg
     7501 catctttaat tggcatctgc tgtttgagca attgtattgg aatcatgctt ttttcttgtt
     7561 gtttaaggtt ttaagcaatt gttcgatatc ttcttcggaa aaagccagcg cccattccag
     7621 acgcttaata acctcgtcag ttttacccgg tccggtataa ataagaggtt cgcattggaa
     7681 tctttcaata aagacccata gatcaagagc aatctcttcc agttcgttca ggaccttttt
     7741 tacatcgtcc agacgattat ctgccaagag cgttttcatt tctcttgctt tttgatttat
     7801 atcgctgatg gtttcattga tgattcctgc aggtaaaaaa tttgtcatcg caaacctctt
     7861 ggctattgtt atttgaatgt atagttcgct aaagcagtaa ctagttcctg cttttatgaa
     7921 tggttcgcca aagccatgtt cacaatgatt tcaaccaact cattgaattc tatgcccgcc
     7981 gctctggcgg catcaggaac cagactcatt tcagtcatgc ccgggattgt atttacttcc
     8041 agaacgaacg gttgaccctg tttatccaat atgaaatcaa ttcgtgcaaa accctggcaa
     8101 cccatcagtt tataaacctg ctggctgatg ctgacaattt tcgccgcact atcctcccct
     8161 atccgggcgg gaataatgtg ttcgcacatg cccttggtat atttggattc ataatcataa
     8221 aaagaattgg cggaagtgat ttcgatcagc ggcaatacct gcggctcgtt attgccaatg
     8281 acggctaccg ttaattcctg cccatcaatg aacgcttcaa ccagaacttc acggctgtat
     8341 tgaaatgctt tttcgatcgc agttatgatt tccttttcat ctttgataat ataagtgccg
     8401 atacttgagc cttgcgttgc ggctttgaca accagaggaa gtccaatcct ggtgagcagc
     8461 tgttcacgaa taacatccgg ctggtcaagc tcgggacgat ttataatttg ataagctgca
     8521 ttcggaattc cctctgaagt gaaaattctc ttgctcaata ctttatcgat gcaaatggca
     8581 ctggttgcca cacctgagcc ggtatagggg atctgcagac tttccagcaa cccttgtacg
     8641 gttccgtctt ccccatattt gccgtgcagc gcgataaaaa ccagatcagg tttatagtca
     8701 atgatgtccc tgatggctgt tctgttgaaa tccagggcct gcgcctcata acctaatgcc
     8761 aacaaggctt cccatatcgc cttgccgctg cgcagggata cgtcgcgttc ctcagacatt
     8821 ccacccatta aaaccagaat tcttttagga ttcaatcttt acaccacctg ttttgtttta
     8881 atatcatatg aaccgttcaa gtaaaagctt accagaaaga tcaattaaat aaagaataca
     8941 gctaaaacat gccttgttca aggcaaggtt tgtgttttat tatactgtat gccgaaatgt
     9001 atggatttat ttgctcacct gggttatatc cagcacgttt tcaaaatcaa aaagccggag
     9061 tcttgaactc cggctttacc tgtcgtttag atggcagggg agacgcgaat cgaacacgca
     9121 accctcggtt ttggagaccg atgctctgcc aattgagcta ctcccctgcg tttgctgaac
     9181 atcagcgata tttatattaa cacataatac aggtcgattc aagcgtctat ttctaataaa
     9241 ttggtttatt aaaccggcat atataaattt aaaccattat ccttctttaa gtttagtctt
     9301 ccctgactga ccaaataatt caagtacccc aaagtttcac cgagcgttac ccggtgaccg
     9361 tgcacatatt cagtcaacct gaacaaatga ggatacaatt ctccggcact catcgggttc
     9421 gtaacggcag tttcaattct atcgagcatt ttttgatgct tggtaatcaa tttatcaatt
     9481 tgcagattta tatctgttat gatcgggccg tgccctggaa aagctttttt gacttccaga
     9541 gcttgtatct tttcaaacag gctaagtgta tcagccagcg gattgttcaa ccaggtattg
     9601 ggccaatcac tgaaatgcaa ataaccactt gcagtcacaa catctgcaac caccaagtat
     9661 ttgtgctgat tcgaccagaa catggcttgt ccgtcagtat gaccgggtgc cgacaaaatt
     9721 gaaaagttat ttccgcccag atcaattgtg tcccctgcgt cgatcggcag gatttctgcc
     9781 ggttcaagat aaagcgggcg aacttctgcg taccagtcat ggatcaactc ttccagcttg
     9841 tcttcaggga attgatttgc cgatatggtt tccatgattg tctggcggta aagctttttg
     9901 aagttggcct gctctaaaaa gataaaattc cgagcccgat cgatttcttt ccgcagcatg
     9961 tagaccgggg catcgctgca ttcctgtaac cagcgagcgg cacccatgtg atcgggatgg
    10021 cagtgggtaa tataaatatg cttgatttgt ttaaacgaaa taccgatttc cctgacggcc
    10081 tgttcccagg tccggcgagt gctttctaaa tcaacgccca catcgatcac agaccatcca
    10141 tcttttgatt cggtcaggaa aacattggtt tctcccagag ggtaaggcat gatttgccgg
    10201 agtacataaa agccggaatc aaaatacata attccactgc gggtcaaatt tttattcgtc
    10261 attgccaaac tccaaatgta ttatattacc actttcgccg gcttccgggc ttagtctctc
    10321 tggttccatt atatgtcttg ccagcgagtc atttctttat gtaatttgtc gctccaattt
    10381 gcattaatta tacaaataaa tacattaaca ggtgctatta ttaacacaag aaaataccat
    10441 taaaaaccat atataaccaa taaatgggaa ggagcgaata tcatgcccta tatggagtgg
    10501 aaattattat tccatgtttt tggattaaag cttttccgct gttgtgaatg cggcagtatt
    10561 tattttcgaa acaatcataa aaccataaga atttggggcg agtcatgttt aaaacacagt
    10621 tatcaacgta ccgatttact tgaacagaga aagcattgct aattggtaga ttctccgttt
    10681 tcgatataaa tactgcgact gggaaaggcc atggagatcc cttccgtctg taaaagccgc
    10741 atgattttca gattgatttc ttcctttacc tttaaattct ctgcccaaac agtggttcgg
    10801 gtaaaatatt gaaccagaat atccaaactg ctctcctgga aattttcaaa agaaaccaaa
    10861 acattatctc cgtggatggt tgactcactt tttaataatt gggtaatctt gtctacggcg
    10921 atttcgattt tgtctggaga agtcagatag tttagcccca gatagaaaga aactctcctc
    10981 ttgcccatgc gcgaccagtt ggtaatcgcc tcgttggcca gggtagagtt gggtacggtt
    11041 accagtgcct gcgcaaacgt gcgaaagcgg gtgctgcgaa aagaaatgtc ttccaccgtt
    11101 ccctccacac tgggggttgt tacccaatcg ccgatagaaa agggtttttc catgatgatg
    11161 acgatgccac cgaaaatatt ggccagggca tccttggctg ccagggcaaa agccagtcca
    11221 cccaaaccaa gaccggtgat aaagccatta atgtcataat cccattcact ggcacagaga
    11281 acgatggcca aagcaataat gacaaagcgg attgctttgg aaaagaaggg caacaaaata
    11341 tcgtcgagct tgaatcgaga tttgatttca tcggaaatgg tggaatgcgt tccagccaat
    11401 tcataaaaac cccaggccaa cataataata atagcacaac gaaacaactt gccaacaaat
    11461 atatcatgga caggcgtcaa aggcaaatac aagagagccc aatacgttcc cagaataata
    11521 attaaatagc gcagtggttt ttgaaacgac tggataattc gattgttaag attggtggag
    11581 tcacgggaaa caagctttaa aactgcactg atgatggtgg tgctgatgaa tttgcccaac
    11641 accaaaaaga atataaatat gagcagggcc atgatgcctg cctgccagga cgcggggaca
    11701 ttctgcatca gatagtgata actggtcgtt aaatattcca tgctgtgggt tgcctcctaa
    11761 gatcattctg tttggataaa gtgtcaatgg agtccaaatc aattcccatg gattcgaatt
    11821 ttgaccggat atcatttatc ttaacatatt attgaacact gccatagcaa agccactccc
    11881 ggaggagtgg ctgtaaatat tttggcctac tgcacgcaac ttatttttct ttttcttcgc
    11941 gttccttttt ggctttggcg ataattttat ccgcatcctg agaaggaact tcttcgtaat
    12001 ggtctacttc cattctgaac ttcccccggc cctgggtaat cgacttgaga tcgatggtat
    12061 atctggccat ttctgccaaa ggcacctggg ctttgaccac ctgtttcttg ccggatggtt
    12121 ccatgcccaa aaccctaccg cgtttgctgt tgaggtcacc gatgatatca cccatgtaag
    12181 tatcgggaat ttctatctcg acatttacga ttggttcaag taaaaccggt ttggctttct
    12241 ccaccccgtt tttaaaagca atcctcgcag ccattttaaa agccatttcg gaggagtcca
    12301 ccgaatggta ggaaccatcg tacagggtgg ctttcagatt ggtcaccgga tatccagcca
    12361 aaactccttc ttccagagct tcctgtaagc ctttttcaac tgccgggaag aagtttcttg
    12421 gtacggagcc gccgaatacg ttttcttcaa aagtaaattc cccttcatca aagggttcga
    12481 atttgatcca tacatgtcca tactgtccat gaccgccgct ttgttttttg tgtttgcctt
    12541 ccacttcaac ggttgtgcga atggtttccc gataaggaat gcgcatttcc cgactgacga
    12601 cgtcaacccc gaacttgcgg gccagtttgt caatcaggat atcaacatgg gtatcgccca
    12661 tggtggttaa aatagtctgc cgggtttcgg tatttttctc tacggtaatg gtaggatctt
    12721 cttccatcag tctctgcagg gcgccgctga gtttgtcctc gtctcctttg ctcttgggct
    12781 cgatggccat gctcaaagtt gggcgcggga agtcaatcgc atccaggatt actggtttgg
    12841 ctttggtaca taacgtatcc gcggttgagg taacgcttaa tttggccacc gctgcgatat
    12901 cgccggcatt cagttccggt acggaagatt gctctttgcc ggtcatgacc agcagttgag
    12961 taattttttc atccttttct ttattggcat tgtagatcac gctgtctgcc tttaatttgc
    13021 ctgtaaaaat gcggaacatg gtcaaacggc caatataagg atcggcaatg gttttgaata
    13081 cttgggcaga aagaggttcg ttttcaatgt ccttgccttt taccagagta ttataatcag
    13141 gcgtcggtga acaagctgcg atgaagtcca tcagcgcagt cacacccatg ttattcatta
    13201 ctgaaccaca catgacaaaa accgccgagc cattggaggc cgcagccttg atgccgtctt
    13261 tgatttcttc attggtgatt tcttcgcctt ccaggtattt ggtcagcaaa tcatcattgg
    13321 cttcggctgc agcttcaatc agctccagct tgtacatctc cacatcgtcc atcatgtcgt
    13381 cagggatgtc agtttccgtc attttgccgc tgcccggttc aatcatgcag gccttcattt
    13441 taattaaatc aacgaaacct ttgaaagtat ctgccgcacc gatcggcaat tgaatcggta
    13501 caacattgtc actgaaagta tccttgattt gcgccaggac tttataaaaa tctgcgtttt
    13561 cacgttccat tttattgatg aaagcaattt tggggatgtg cggataatct tcccaggcca
    13621 cctgtgtctg tacttcaaca ccggcggtgg cggagaatag catcagcata ctgtccacga
    13681 cccgcatggc tcctgcgact tcaaagtaaa agtcagcgta tccgggtgtg tcaacgagat
    13741 tgatcttaca gtctttgtat tcacatggaa tcagcgaagc gctgattgta aattttttct
    13801 tgacttcttc cggcaggtaa tcccccacgc tggtgccatc gtcaaccttg cccaaccgct
    13861 tcaggacgcc ggtggaataa agcattgcct cagccagtga tgttttgcct gtacctccat
    13921 ggctgacaag tgctatgtta cgaatactac tggtaggata gaccttcatc caataaacct
    13981 cctcaaaata attgtacact gtaaaatggt actatacttt tgcacaactg gcaaaacctt
    14041 tttaatttgt ttcattataa ggcgccgctt tttccaagtt tgagttggca agcagcgcaa
    14101 gtaagaggga gaagctattg actgatttcc aaatcaggaa aatttctttt ttgaaaactg
    14161 acagccagac ttttgtattt gtgaatcagc tgataattgg tcatatcaag attaatgggg
    14221 gtcacactga cgtaattatg ttccaccgca tacacgtcac tgtccgggtc ctgttcttcc
    14281 tgccgcaggc ctccccccaa ccagtaatac gtgttgccgc gcggatcttg cctttcttca
    14341 aaaaggttgt catagtttct caggcccagt ttacagatgc ggatgccttt gatttgggat
    14401 cgctccactg ccggtatatt gatgttgatc aaagttttgg gttcaattcc ccaatgcatc
    14461 agagcgctca ccaattggcg cgttaaatgg acggcaaaac tgaaatcgct ttcataatgg
    14521 tcagagtcaa gtgaaatagc taaagatgga catcccatga tgactgcttc ggcagcggcc
    14581 gaaaccgtac cggaatacaa gacatcactg cccagattgg aaccgtgatt gatgccggaa
    14641 ataaccagat caatattttc ttcgattaat cgggcgatgg caattttgac acagtctacc
    14701 ggggtgccgc tgacgatcca ggcctggctg acccctggga aagtcgtctt ggtaacctta
    14761 attggatcaa atacagtgat agaatgtccg gtgccactgc gttcgcgatc aggtgcaacc
    14821 agatataact ccgcaatctg gctgagttcc tgaaccagga tttgaatgcc gcgggcatga
    14881 atgccatcat cattggtaat cagaattctc attcaaagct aactcctcgt tcaaacagtt
    14941 tgatttcttt gattggccaa taaataacaa aggctttgcc tttgattcta tcctgactca
    15001 tccaggcgtt ccacatatgg ctgtcaaaac tgtgattgcg attgtctccc aaaaccagca
    15061 ggctgttctc gggtaccgtc accggcccaa acgtatagtt gactggttcg gcaagatatg
    15121 gttccaccaa cggttgatca tctatgtaga cacgattgtt tttgatttca accttttcgc
    15181 cgggcaaccc aatgactctt ttaatatagt catctttgga ctgcaaaaca tctggcggag
    15241 cgaaaacaat gatttcgccg cgctgaggtt gcttgaatcg ataaataaat ttgttgacca
    15301 tgacccgatc acctactttt aatgtaggca gcattgattc tgaggggata atccggcctt
    15361 cgataacaaa ggttcgtaaa atcatggcca ggataaatgc aatgattaca atactcagtg
    15421 tttccttgaa aaactctttt atttctttat tcaatcagtc caccccaata caatttatcc
    15481 cttagaaatg aagaaacagg tcgaaaagct gcatgggcag ctaaaactca tagattttta
    15541 tagtcatttg cccggcttgt ttgtctttgc tcaagccaaa catgatattt ttgtctttaa
    15601 gacttttatt caagaaatca attacataaa ccagttcgtt atgggcggaa aaattcttaa
    15661 gcgcgattag ttctagcttt cctgtatctt tttgcgactc ctccacctgc cgatctcctt
    15721 tcttccatac cattccaggt cagtaaatgt ttgtgcaatc tttgatgatt acgcctgtgt
    15781 ttaaacgccg cgcaccttgc agcgcgcttc atggatatgt tccagttcac ggcggtattt
    15841 cagcattatt gtatctgcaa aggaatttat gtcactgata cccagttcgc tcaagcccac
    15901 ggttttgatt ggcgtgcctt ctgtttcgat attcaaacca atatgaatca atgatgatac
    15961 attggataca gttgcaaccg aaacagacag cttggctttg ttaaaataaa gatcatcacc
    16021 caggcgatag acatttactt caaattgact tagttcttcc ttgatcaaag tcataaacag
    16081 ccattgccgg taaatagcca gttccagatt gggattaaag tgttcgatga ggaaattaag
    16141 catcagcgga gagtaaatcg attccttccg gctgacgtcg attaaatcga tcatcttgtc
    16201 cagatcaacc tttacttgcc cgataaaggc caccactgca tcaccaacca gcccgaattg
    16261 gttgtgtatc cagtgagatt gcatttgcgt cccgtcatat gttatgcgct tgtcgctgac
    16321 taatgtttta atcaccctta tcatcctctc ttttatcatt cctctgccgg gatttgaata
    16381 tattataaat attaccacgt aagccgaaat tataaaaact tctgcgtaaa aaaaccggct
    16441 gctttttgcg gctgtctgca gacagattaa tgttcagagc agtcggggtg atgtatatta
    16501 tgctatggca atcgtctcct cgggatggaa aagataaaga taactttctt taaccttttt
    16561 ttgcaggatt gattccagtg cttgtctata catattaatc tgaacagtgt attgcgcgat
    16621 taaagcagcg cggttttgcg cactgatata atcggttttg taatcgacca ggacgagatc
    16681 atctccttcg acaaaataca ggtcaatgac cccctgcaca agcaattctt cgtcaccggc
    16741 attgatgtct tccagcactt gtgcagcttt gcaaaccaaa ttgaaaggta cttcgcgata
    16801 tacttgggaa gaatgcagca ttcgctgtcc cagagaggag gcgaagaaat gtgctatttt
    16861 ttcagtttga accgccctca tttcatcctc gaccaacaac ccctgcacga tcatctcctg
    16921 taattggcaa tcaatttctg tcgtgctgcc aaccctggtc aggtccagat actgcataac
    16981 caaatgcatg atggtcccct tttctgctcc gcttaaagca gcagtactgt ccggtgggtt
    17041 tataaaaaca ggtcccggaa gcagcagggg gatgtcaggc agattggccg gccaattatt
    17101 gtttttcagc tttttgatac tgctgactga tcgtttggag ggaatcgttt cggctgcccg
    17161 gtacggataa tcccagttta aacgatggtt gattaaagct gcttcagggg acggcgtttc
    17221 tctcttgaaa ttctgcagtt cttgtagaac agttgatgcc tgcgacggtg cttctgccag
    17281 ggatggacca aatgctgccg ggcttgcgat ttttatatcc caacttgaat catcccggat
    17341 aatctggcgg ggatcaaact cttccgccag caggtcacgc agaatctgac cgtcaggatg
    17401 tcgcagcagt accggaccta tccaatccat aaaagtattg gctttactga gaccatacag
    17461 gttcaaggag ctgctccatt tttgcgtcag tgctggcagg ttcttcagtg aacccagcag
    17521 aatcagtttg ttgattgccc gggtacagcc cacataaaga atacgcatct cttctgaaag
    17581 gttttctgtc tttaattggc cctggatggc tatgcgcgcc aggctgttgc attttacgcg
    17641 ggcttgcgga tcgacgaaca ttggccctat gcccagatct ttgtgaaaca gcaccgcagc
    17701 gttggtatca ctgagattaa attgtttgcc cagaccggcc atgataacca ccgggaactc
    17761 caaacccttg ctcttgtgaa tactcatgat tcgcaccaca ttatcttttt cactcaacaa
    17821 cctggcgttg cccatgtcgc cgccccggtc ggtctgaaga gaatccacaa aacgaagaaa
    17881 gttatagagt ccttttaaac tactggtttg gaattgacgg gcacgatcca gcagaatcct
    17941 caaattggct tgacgctgca gaccaccggg catggcgctg acataataat aataatccgt
    18001 ctccagcatg atttcccaga tcaattcatc aatgggcata aagcgggctt tgtttttcca
    18061 atggctgatc tgctcccaaa aacaagtcaa tcgccggctg agatcatcag actggttttg
    18121 catacattcc tctgctgctt gataaagata tgcagcgcgg ctgttccgcc ggatatcgat
    18181 taaatcttca agggtaaatt tcgccaaagg cgagcgcagc acgctgagca gcgggatatc
    18241 ctgttttttg ttgtcaatga tacgcagaag atttaaaaac atttcgactt ccagattttc
    18301 aaaatagccg ttgtttacat cggcataaac gggtatgccc tgggcagaca gtgtatccag
    18361 atagacgtct gctctatttt tggtggagcg catcaagata accatatccc gatattgcaa
    18421 gggtcgatga catttttccc gtggatcata aatggtcaga ccaaccagtt ggtgaatacg
    18481 ttgagcagcc aaacgggctt cgatctcaat gtcagaaagt tcctcctgat cttcgtcaag
    18541 ttcaagtaat gctgggtcct tttccagcag caagacttcg atacgggcat cttcctgggc
    18601 ttccatgttg ctgccgtaat aaagataaca ggattcatca taatcaattt ctcccaaggg
    18661 ctcagacatg atgtgtttga aaagataatt aatcccatcc agaatctcct gccggctgcg
    18721 aaaatttttg cccaaatcga tgcgacggtt aagtgctgac ggttcggata caaatgtatt
    18781 atatttagcg ataaaaaggg ttgggtcggc cagacgaaat cgatatatgc tttgttttac
    18841 atcaccgacc ataaaaaggt tgtgctcgct tttgatagat tgcagaagtg tttcctgtac
    18901 cagattgctg tcctggtatt catcaacaaa aatatattgg tacttttcac ggtattggcg
    18961 ggcacaatca ggattgctca acacctgcag ggcataatgc tccaaatcgt taaaatccag
    19021 catgcctttt tcagcttttt tctcctgata ccggcgatta aaatcagcaa ccagttcaac
    19081 caaggcttgc atggcggatg acatttgccg cagttctgcc ataaactggg caatgttttt
    19141 gccttgcgta atccgggtaa gttcctggag tatttttttt ccttcattgc gcagcccctt
    19201 ggccctctcc tgcaggttgg gatcgcaatc ccgggcaacc ctgcccaaac gggtaaaatg
    19261 aagcgcggtt attttatctg tcagtgcagg caagcccaac cgcgccgcat gtgtcaattg
    19321 gccggtctgt accaggtcat cttccaaggc cgggcgataa ccttccaggc tggcatgttg
    19381 atcgattaaa gcaaacgcag ccgcaaaaag atcgtcggcc gcattgatcc tggactgcat
    19441 atcccggagg atattgtcag cccactgact ttcgacgaat tcttcttcag tcaaggcaaa
    19501 gatacgaatc ttttcggtca gccatgccaa aggttcaggt tgactttgaa taaaattatg
    19561 ggtacgcagg attaaatctt cgaccgcctg atcgctcctg ttcccggcaa accgttctac
    19621 caaatcgaga aaatgcgcag agccgttatc ataagcttct tccagaatct cttccacggc
    19681 ttcccgccgc agaatcgaac attcggctgt atccccaatt ctgaacttgg gatcgatgtc
    19741 cagcaggtga aaattgtcgc gcacgatact ggtgcagaag gcatgcagcg tgcaaattga
    19801 actgcggcca atcatctgca gttgataacg caagtgatct tgatggtcag gcatcgcttc
    19861 gatctcttta ataatggcag cggtgatttt ttcccgcatc tctcccgctg cagcattggt
    19921 aaacgtgacg atcagcaggt tttcaatact caaaccatcc cggcagatca tacggatgat
    19981 acgttcaacc agaaccgctg ttttgccgga accggcggca gcagtgacca gcaaattgct
    20041 gtcacgtgca tcaatcgctt gcagttgttc ttttgtccag ttattcattt gtatcgttcc
    20101 tccggagttg cttgattttt tccaaaatcg cggcatcttt ttcgggcttg atcaatttgt
    20161 agcgattggc ggtaaacagc ttgtcaaact gacagatgga atggtagaga caatagtcgc
    20221 aagctttgcc ttgcgcagtt ttgactggtt cgatacagat atggccgctg atcattcggg
    20281 tacctaattg ttggagcaaa tcctgcaagt aacgcagaag caaactaaag tcttccaaag
    20341 ttagcacgtt ggatcgacta ttgaattccc catcattttt aatgccgacc ggcaggatgt
    20401 cggaatctcc ttgaacagca ttgtccattg cacgtaccag ggcaacatct ttgagtacaa
    20461 gacccttcat acgcagtttt ttgcggatcg acgcttcaat ctctgccggt tccatcgccg
    20521 tcgtattgat gagaggatca tcgattttga aataaaacag gccggccgga cgatacttgt
    20581 ggctggccgg atcagcctga tgagccgtag ggttggattg cagcaccgcc agcaaataca
    20641 caaacaactg cagatttaaa ccataataga gttcggaaag actaagttgg cgatcgctgc
    20701 ttttgtaatc aatgattttg caataagtat gctcccctgt ctccaggata tcaatccggt
    20761 cgatacgtcc ttccagataa aaggtttgct gttggtttaa ttcaatttta atgggtggaa
    20821 aagccccgtc cgagccaaag cgtgcctcgt aaagataagg ctcaaaatca ccacgctgaa
    20881 tgtgttcggt caaggtccag acagcccggc gggtaatcct ctgcagacgt cgggccagat
    20941 aacgatagcg ggaggaactc atcataattc cattattatg aacggtcagc tgttccgtca
    21001 taatcttgtc tacaagttca ttacaggcag cgtgatccag cgttctccaa tcgatgccct
    21061 gtttgtcaag ttcgcaggca aaaatcgcca gtccttcatg cagcagactt cccacttccg
    21121 gcatacgtac agcaaactct ttgcgttcct gggggcgcaa gccatagcgt acaaaatgtg
    21181 caaagggaca ggcggcaaat tgttccagcc gggatacact ggaataaaaa ggacttggat
    21241 aaagtttgct ggcactgcca ggttcctgcc cggtgatttg attttgataa aaaagagcgt
    21301 ccaacagctc atggcgccgc tgatcccaag cggaattatt ttcataccag gcatagacgt
    21361 cccaccaaaa atcacagatt tcttccccat ccagataaag ccgcagtttt tcaatcaaat
    21421 atttatagct accagaagac gtcaccactt gttccaattc gatttgccgg tcccgcacca
    21481 gatcactgga tacaatgatt ttcgggaaca agcttttcat ccggttgatc aacacggagg
    21541 ggcgcagcgc cctgccttcc tgatcggcta tggcatacga cagggaaaga aacgcctcgg
    21601 cttgattaaa ggcctgataa atagttactt gctctgtcag gctttgggtg gcctgatccc
    21661 aatccagatc gatttgacat gtctgcagca acattatttc attttccgtc aataacattt
    21721 gactcgcctt acccgcaggc agaatgtcat cattgacgcc catgatcagt accgctttaa
    21781 aggcatgact tttgctgcga tgaagactgc cgatcacaac ctggtcgaca gtggtgggaa
    21841 tgatcccaat ggaaattgag gcaaacccgg tttccaacag gttggcgaat tcctgggcac
    21901 gaatcttttg atcccccagg atttcaacca attgatccag cgtttgcata acaatattcc
    21961 atatttgtgc attttgcagc gcatattcaa acaaaccatc cccgcgcagc tgctctgtcc
    22021 aatcctgcag tttggcttcc atctgcagga cttgcaggta atgataaagc gcctggccaa
    22081 tttcctgccc ggtctgcact cccgccaatg ctttttccaa ggtttgcagc ggcataaaca
    22141 gctggcggcg cagtatattg agggctgtca attgttctgc accaatctcg tcctgcccgc
    22201 gggtaaaatc ttcccgccac agcttgccgc gaatgccgta ggccagcaga tagttttcca
    22261 tctcctcact ttgatcttcg ctcagatcag tgaaaccaca tttcaacaac atcaaaatat
    22321 cctccggccg ataacctcgt gtgacaatcg ccagagaagc cagtatcatt ttaatgagcg
    22381 ggtgattgtc gatggacgat ttggcgtcaa gaaagaacgg gatccggtac tcgttgaaga
    22441 ctctggcaat catggggcca tatgtttcca tattgttgcc cagcaccgca atatcacgcc
    22501 atcgccagcc acgttcctgg actagtctga tgatactgcc tgccacatgc tccacttcgc
    22561 tgttgggatt agcggctgca aacaacgcga tcgcatcagt ttctgcctga aaagatcggt
    22621 aggggtaggc atacagttcc tgttccaggt gcttaagact ggcagtgcgg ggcattgcca
    22681 gccagcggtg tgattcggca gcgagatcaa tgattttctc ctccaaacca tgttggacgg
    22741 ccagggcatg gatgttctta tagcaacgct tactcatttt aaaaagatcc ctgtcccgtc
    22801 cgggatgcag gtcatcgatg agacatattg ttgtttcttt ggcttgcagc atgagcttct
    22861 ctatgatccg taaatcctgt ggcggaaaat ggtcaaattt atcaatccag atctgacagt
    22921 tggtcaatag tttggaatca tccagttttt caataaataa gttgagctga tcctccatgt
    22981 caaggtagtg atcggccatg taatcagtgt aggcagtaaa aataagggta atgtcctgca
    23041 tttttaaacg cagggaatct tctctgatct cgttcactct ttcccgcaag gcggcaggat
    23101 ccaagtcata ctgtttgaat ccggtcaaaa taccggcgat ttcttctgca aaaccttgct
    23161 gatgacaagc ttgctgataa acggtcagat cactggcaac attatgaatc accttcttga
    23221 tgaccatact tctgccctgt tcgttgatta atacgtggcg atgactgccg gtttcattca
    23281 aaaccctgta taacaaacgg ttaaagctaa tcacttccgc ccgcatgatc cctggtaaat
    23341 tgagttcttt gatcaaattt cgttctgctt gcagggtata ttgttccggg accagcaaat
    23401 aaaggcgctg atcactgtcc tggttaagtt cccggccgat ttccatcatg gccaggcgat
    23461 cggttcccct tcctgcccgt ccgatgatat aacgaatggt catggttgcc tgtcctcccc
    23521 attaaatctg cgctgacaaa caagcagatg attataacat tcgcgtcagc aaatggaata
    23581 tcctgcccaa catgacattt gacacatcaa acagacccat gatcaatcgt tattcagtta
    23641 tactcttaat gataagcctc tgcttactcc aaaagtaaaa tacgcgcgcc ggccgccagc
    23701 aagacgacgc caagcaagtc cagatagtga aaggacattt ttttcatgcc gaatagtcca
    23761 aagttgtcga ttaacaaagc ggtcagcacc tgggcgacaa tgatagcggt ggtggcattg
    23821 cctacaccaa tttgaggcat acagcgcaca atcgcataa
//
LOCUS       DAOTHZ010000030        23485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2541_length_23485_cov_7.169996, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000030 DAOTHZ010000000
VERSION     DAOTHZ010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 23485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23485
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2541_length_23485_cov_7.169996"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1869
                     /locus_tag="P5549_05200"
     CDS             184..1869
                     /locus_tag="P5549_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW12500.1"
                     /translation="MSNEEKQNGLSRRTFIKGAAVSSVMLGSAGALIGCGKEEEAPAP
                     KEPNTESAIPSFLKKPDAIPDADIKQTVAADVVVIGAGMSGLCAAMAAAEEGAKVVLL
                     EKTDRVHFRGNDYGAIGSKMQKQINNEINKMEAVQEIMRYNAYKANQRVVSLWADNSG
                     MVADWIMGKAEGYGCKPKPVPLDETVTPGTTIKGFPTLSFRMDPSEAAINDAPKGTDP
                     WTASMRYALKKGCEDAGVQFHYKMPAVQLVRQDKGRVTAVIAGEKGNYTKFEGKKGII
                     VCSGDYNANKEMREYYIPSTKYIDANMYEMVDGKQNTGDGQLMGMWVGAAIDEFPHAP
                     MYFDFAVPGAPILADALMRQPWLNVNVRGFRYCNEELPYAYLCNAERQQPEQARWAIW
                     DAKWPEEAPSFKLVACKDMRTNFHNPESVKAYVEKGYIRSADTLEALADMIKVPKDNF
                     MKTVARYNELAKLGEDIDYGKRAACLTTIEKPTFYAAPLGTALLVTLGGLQVNEQLQV
                     LDEEKSVIPGLYAAGNASGSYYSNDYCVNLPGNSHGRAYTFGYLAGKNIVKLG"
     gene            complement(1958..3994)
                     /locus_tag="P5549_05205"
     CDS             complement(1958..3994)
                     /locus_tag="P5549_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12501.1"
                     /translation="MKLENALSPMIRNRGVLGNNYDSEDIDLINQIKKLKLDVINKRI
                     DKQEIHLIRPEVVDSWIRSFDNGLNVFEYNYAPALKGKVLQQRCQEKATLLDSSEPFI
                     HQLASMLADSQCIILLSDEQGCMLRVLEGNRKLEDQNKRFKLVPGSIWNESTVGTCAH
                     AISLIHEIPMQICGPEHYCEKYDNIFCSAAPIFDGNQNLTGTLSIVSPSFHQQNAHSL
                     GLAVSMAWAIQRDIQLLFNKELLDVTMNSTDIGVLMINKGGIITQANAIAQGFFDKLL
                     EHDVIGMPYKTILGQQAAVESVLNGGKPVYDSEIHLERWDQTIHLLSAQQIADSVGQH
                     LGCMVTFKKFAQAKKNAVPATTLEARYDFTKIIGDSEGIKHCIDLSQKFSGLEANILL
                     QGESGTGKEMFAHAIHKDSRPNGPFVAVNCAAIPSTLIESELFGYAGGSFTGAEKQGR
                     PGKIELADQGTLFLDEIGDMPLELQPVLLRVLDEKRVMRIGASQYVPVDFRLITATNR
                     DLDDLVEKDLFRKDLLYRLKVLQINIPPLRQRGEDIVLLARYFISIIAQKQRIKAPEF
                     SDELLIALLNYAWPGNVRQLENAVLYALNVSNGSLIETSHLPEDIKASLAAFHAKQRK
                     PHVPVNHLSMKEVEKIFIKEALLQCNHNISEAAHLLKMSRATLYRKIKEYQLVK"
     gene            4245..5468
                     /locus_tag="P5549_05210"
     CDS             4245..5468
                     /locus_tag="P5549_05210"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRW12502.1"
                     /translation="MVTAIKSSEMLIEGVLDNGLVFIGEYLESILRHTILIADQNGKI
                     YFPESNQHMLDLDSSFVGISAEMVTRESFYCEESHHLYYCVETKHQRAYVIVKDLNTE
                     WVPRILEILRESRLPIKCYFSKINKSKEMFTAELKDYLFGAGQADIRDILYLCDSELD
                     LQRPYFALLLCAEEGCLKTDPEIIHSYVCEHLRQEKCKTISVYHDHQWVFMIPAQIKD
                     DCRINEMENEFDLNALKRAIENKFKISASMGVGQVYALSSLRKSFREARMTMILNHLT
                     GLQSFIQKFSDLGIYRPIFSQEMNHIYKYCSCTLGKLMEHDCKYDGELLPTLRKLLDT
                     CGNNKATADQLFIHVNTLYYRINKIEQILDMDLSKMHARAELYTAIKVWDTLQVLNGK
                     EEMTDTLPAILAMQA"
     gene            5636..6976
                     /gene="miaB"
                     /locus_tag="P5549_05215"
     CDS             5636..6976
                     /gene="miaB"
                     /locus_tag="P5549_05215"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRW12503.1"
                     /translation="MKHDQAPPRGYHILTYGCQMNSRDSEIISGLLEQAGYCEVPLEA
                     ADLVVFNTCSVRHSAENKVYGKLGEVNKIKRSKPGLRIAFGGCMAQLPEVVKRLKRQG
                     VDVVFGTHNLHQLPILLERIDAGEKSVIEVWEQHGEIIEDLPSKRKKSVSAFVNIMYG
                     CNNFCSYCIVPYTRGRERSRKARDIIWELEKMVGEGVKEVTLLGQNVNSYGQGGDESL
                     DFASLLQMADKVEGLQRIRFMTSHPKDMSDALIDTIAAGEKLCEHIHVPLQAGSNQVL
                     KRMNRGYTRESYLELINKMRARIPGVAITSDLIVGFPGESEADFDDTLEMVERVRFDA
                     AFTFMYSVRTGTRAASFNDQIELAVKQNRLLRLNQQQYDIALQKNQALEGRVMEILVD
                     GPSKTNPDRLTGRTRTNLIVIFSGPNDLIGQLINVKITTGKTFSVFGELPAETS"
     gene            7044..7433
                     /locus_tag="P5549_05220"
     CDS             7044..7433
                     /locus_tag="P5549_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRW12504.1"
                     /translation="MTTEEIIKMAYELGNAIAQSKEVDALREMQVKLGDDADAFGLIM
                     RYQEAQTQMENKLQDGLQVTAAEEKNIEIMEEEINNNPLIQELIKVQEKFDSLMQGVY
                     FAMNQAISGSDTCSSGCDSCGGGCGVM"
     gene            7517..10090
                     /gene="mutS"
                     /locus_tag="P5549_05225"
     CDS             7517..10090
                     /gene="mutS"
                     /locus_tag="P5549_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRW12505.1"
                     /translation="MAKFTPMIEQYLEVKANHQDAILFFRLGDFYEMFFEDAVLAARE
                     LEIVLTSRDGGQQKVPMCGIPYHAVNNYLPRLISRGYKVAICEQVEDPKQASGIVKRE
                     VIRIISPGTILEDFMLDEGANNYLAAVDIYEKTIGFAYIDISTGEFQFTEINGPHARQ
                     DLYAELHRVAPAECLLSARLTRNSLWEEQPLGNDFLFSRFPEDDLDLDQSRAVMADHF
                     GSAWLDEGSISACHAGCLAAGMIIRFLYNTQKSRLLHIDGLQFYQSSDYMELDSFTRR
                     NLELTSTIRQGRKEGSLLDIIDQCRTPMGKRNLRRWLEQPLMGMEAINQRLDGVEELA
                     ADIGLRQSGRDAVNKMYDLERLSGKLGSPLLNPRDLLALKHSLALLPAIKGLLCNCNS
                     QILRAIAELEPLAEVHEIIHSAIDDEAPLTVREGGIIKSGYHAEIDELKSLGVQGKNY
                     LLEYENREKLRTGIKHLKVSYNKVFGYYIEVSKSNLNLVPADYIRKQTLVNTERFITE
                     ELKNYEDKILGAHDKLCVLEYDCFIEIKHNLEPFIPVLQASAKAVADFDSLLSLAQVA
                     FLNDYVRPRLSRDGRLEIKAGRHPVVEKSLLDTRFVPNDIMLDNRDNRFAIITGPNMG
                     GKSTYMRQAALINIMAQMGSFVPASEARISIVDRVFTRVGAADDLSAGQSTFMVEMVE
                     LAHILNHATANSLIILDEIGRGTSTYDGLSIAQAVSEYIHDKVKARTLFATHYHELTR
                     LAEEKKGIINLSVSVMESVDTVTFLKKVLPGKADKSYGIHVAQMAGLPRTVTDRAYEV
                     LAALESNEALPRHPEVQQISLFAEGPNEILEELDHLDLDNLSAREALMILYHWKEKRR
                     G"
     gene            10095..11789
                     /locus_tag="P5549_05230"
     CDS             10095..11789
                     /locus_tag="P5549_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRW12506.1"
                     /translation="MLIGIDVGGTFTDGVLFDNQQSRVVKTVKVPTRNEQLQSSLLAV
                     LDALLPGQERGGIQRIVLSTTLVTNLLATNTAEPPALILIPGPGLPFEYLLTFPHSYI
                     VKGSIDFRGRETEKLDRQEIEGVIETIRAAGLHKIAVVGKFSNRNPQHEQSVRQIIMD
                     KFPDAEVGIGSELAGQLNFMRRITTSYYALMTRQRWTSFALEIEKAIRERGLDKVPLD
                     ILKADGGTMPLRNALQYPCETILSGPAASTMGAKAITGQNTNAVVLDIGGTTTDISLL
                     LDGQPLYASRGACINGKYTHIKAISTRSLPLGGDSPVCFEPQKGVYIHPIRRGPAVCF
                     GGECPTVTDIFNCKYDLNIGDAGQSSQSLQRLQKEAGLTLPELLELVEHDVMQQLMEA
                     LQNIFMEWENEPAYRVWEVVHNRKFKVKRIIGIGAAASAIVPMLAAMNKVNSFIHELS
                     PIANALGAALARPTLAFNMHIDTQKGSYFTDYDGVGGDLPRGQSMQMVDAHKLAREVM
                     QKLAEQRGLGEYAEEAEFVREEQFNMIREWDRIGKIFELGMQIKPGFIKEFEEGSL"
     gene            11786..13138
                     /locus_tag="P5549_05235"
     CDS             11786..13138
                     /locus_tag="P5549_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRW12507.1"
                     /translation="MKNTGVIFFPAFDWAISPTHPEREERLLYTRDQIFEEGIMDLPN
                     IHEFKPRLADKKDIARVHFCVPNVEAQITDAHLVSAGSALVIADAVLRGEVDNGFSIA
                     RPPGHHAMTVAHGNRGFCNINNEAIMIEYIRKQYGLRKIAVVDTDVHHGDGSQEIFYD
                     DPDILFISFHQDGRTLYPGTGFVYELGGPMAWGSTINIPLPPQTTDEGILRVLDKLVL
                     PVLEEFAPELIVNSAGQDNHYTDPLANMRFTARGYAELNRRLAPDIAVLEGGYSVETA
                     LPYVNMALLMAMAGIDYANLHEPDYRPEAFKESAKNLEYIEQIISHQLFNFRSRQQKI
                     EENRKKYGHEYGEHQQIYYDTEGISEDQARRLRICPACPGWRRIDSSARHRDGKTYHV
                     FCISIPTFACPECQKEAFNAYQDMAMYNHHYDYIYLQDRAKDRYIALNVANGREEVYE
                     "
     gene            13139..14881
                     /gene="mutL"
                     /locus_tag="P5549_05240"
     CDS             13139..14881
                     /gene="mutL"
                     /locus_tag="P5549_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRW12508.1"
                     /translation="MSIKLLDDNLINQIAAGEVIERPSSVVKELVENAIDAGSTRIQV
                     KISGGGLEVIEVEDNGCGIPESDIELALQRHATSKIALPDDLFNISTMGFRGEALPSI
                     ASIARLEVYSRQKDGIGAQLQLDGGTIISRQPYAGTEGTRIVVRELFYNTPVRKKFIK
                     STVAEQTHIYETISRLALSRPDISFLFASEKKMYFKTPGSGVLRDALIAINGKDYADH
                     FIDFHVRGEKYSVAGLLSKPEFRRQNRKNQIFFVNRRLIKSPMLNRAVDEGYRGLLLA
                     REYPGVILYLTAHCQEVDINVHPQKSEVRFRDESEIFRVVSRAIKDAVNGLAYTMGEA
                     AWAERIAEPEAIFKPEVNPGRNGAYIPPPRAFEQSFTFVYPRDNQAAAGSPDRLADEL
                     PAQSPFRLIGQCFNAYLLVETEGRLWLIDQHAAHERIMYNQLRRQIAAAPVATQALAF
                     PLGLDLSAAQIDMLEANRAVFERVGLYYDILGPDSIAIRSAPPALHGQESELLLEILE
                     MIAAEQCPDIHDEAICMMACKQAIKANQLLSRAEMEKLVSELLQQENYLHCPHGRPSF
                     MEISRSDLDRRFKR"
     gene            15240..16013
                     /locus_tag="P5549_05245"
     CDS             15240..16013
                     /locus_tag="P5549_05245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRW12509.1"
                     /translation="MELIVTTTQRIKEKEADLEEFLAESGLQFVPRDRSLEKLREKYQ
                     AAGVVVWGAEVPILYFENEKLYFHPSMAKNRIAFYRKQQRPDIMIEAAGLERGDSFLD
                     CTLGLGADAIVASYFSGHGRVTGLEHQPEIAWVIRWGMKRYCNEMIWLQEAIRRVEVV
                     AADHKEYLPGLPDWSYDIVYFDPMFPHPIFKSQPISPLRKLADHDRLEAASIKQACRV
                     ARKRVVMKDLAEGEELERLGFQKISRSKYNKLAYGVIYV"
     gene            16060..16986
                     /gene="miaA"
                     /locus_tag="P5549_05250"
     CDS             16060..16986
                     /gene="miaA"
                     /locus_tag="P5549_05250"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRW12510.1"
                     /translation="MKFYALVGPTAVGKTALSIALAQQIDAEIISCDSMQVYRGMDIG
                     TAKATVEERKIVLHHMIDIVDPDVSFTVADYQRMAREEILRLNQNGKPALLVGGTGLY
                     YQALVDDFHFFPLESRDQVRQKWETVCQEKGQAFLFQELRRVDPVYAEKVGANDRKRI
                     IRALEVWELTGEPFSCLQRKNQHSHQVQVVGLYVEREVLYHRIEARVDKMIEEGLVEE
                     VLRLRTQGYDSSLKSMNSLGYKQVNAYLDGMLNFQDMLYEIKRETRHFAKRQLTWFRK
                     DQRIVWYDAGQEQTVDLLAKIRNGLAGQNRLV"
     gene            17043..17294
                     /gene="hfq"
                     /locus_tag="P5549_05255"
     CDS             17043..17294
                     /gene="hfq"
                     /locus_tag="P5549_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRW12511.1"
                     /translation="MMKNQMNLQDAYLNQIRKDKILVTIYMINGFQIKGVVKGFDNFT
                     VIVELDGRQQMVYKHAISTISPFKPIGVINFENKNEEEA"
     gene            17333..17461
                     /locus_tag="P5549_05260"
     CDS             17333..17461
                     /locus_tag="P5549_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12512.1"
                     /translation="MNTIEIIMGIILLLIILMIGDIKKKVIALIIFAAVVAEKLLH"
     gene            17631..18635
                     /locus_tag="P5549_05265"
     CDS             17631..18635
                     /locus_tag="P5549_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRW12513.1"
                     /translation="MEIFQWLNNQLLKMEWLSDLIRKLVENGLNLDMSTRLGGSIHFF
                     IYDVIKIFILLAVLIFTISYIQSFFPPERTRAIVGKYKGFWANLFGALLGTVTPFCSC
                     SSIPLFIGFTSAGLPIGVTFSFLVSSPLVDLASVLLLASIFNWKIAIAYVLAGLVLAV
                     ASGTLISRLKLESYVQPFVYGNESFEYAPIKMTTQERIYFAKAQVRDIVGRVWLYILI
                     GVAIGAAIHNWIPQNIISAVLGQDKWYSVLVATAAGIPMYADIFGTLPIAEALVYKGV
                     GIGTVLSFMMGVTALSLPSLIMLSQVVKPRLLAIFFTLVTLGIIVIGYTFNALGHFLI
                     "
     gene            18726..19073
                     /locus_tag="P5549_05270"
     CDS             18726..19073
                     /locus_tag="P5549_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRW12514.1"
                     /translation="MFETVFKALGEPTRLKIIKLLAGRDLCVCDLEEVMQISQPRISQ
                     HLKVLKYAGLVNERKEGQRNICSLNRDQLNRIWDGFQRYIEQPLENMAEFADECARMP
                     AEMGDYCANKKGD"
     gene            19177..19419
                     /locus_tag="P5549_05275"
     CDS             19177..19419
                     /locus_tag="P5549_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRW12515.1"
                     /translation="MKIEVLGAGCARCNQMEKDVINALAELDIAADVTKVKDISKIMA
                     YKVMSTPALVIDGQVKIAGRVPSKKELHSLLKDAQK"
     gene            19661..20578
                     /locus_tag="P5549_05280"
     CDS             19661..20578
                     /locus_tag="P5549_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRW12516.1"
                     /translation="MKNRKMKWLPLLLVMALLAGLVAGCSSAKEQEPAANPPVQQEVK
                     VVSIEEVAAKYFAENPESNRVMPEAALKERLDANDSQLFVVDIRSAEDYAAGHIKGAV
                     NAPFGKMHEFLDKLPADKEIVLYCKSGQTAGQTVAIVNMYGYKAVSLNLGFDNGWVKK
                     NNFPVDTTPVEFPANLTPAKPDANIAKILKDFYVNMPANTNMIAPADLLAKIDSGEDI
                     QIVDLRKAEDFAKGHVKNAINIPFGQLGQNYSKISTEKPVFVYCYSGQTAGQSVAVLK
                     VLGYDAFTLKGGYNFGWTTEGMPVMQEAA"
     gene            20673..21797
                     /gene="rfbB"
                     /locus_tag="P5549_05285"
     CDS             20673..21797
                     /gene="rfbB"
                     /locus_tag="P5549_05285"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRW12517.1"
                     /translation="MREKRQVVKVLDTYLITGGAGFIGSHLIDLLLSKKQPQVRVINL
                     DNLSYAGTRPYPENHVNENNYRFIKADICDSAAVEEIFARYKPVKVIHLAAESHVDRS
                     IIDPLIFVRTNVTGTQILLEAARRFATELFLQVSTDEVYGASTLDHPCSETSPLNPSS
                     PYAASKAAADLLVQAYQRTYQLPVIISRCTNNYGPRQFPEKLIPLVIAHALHGKPIPV
                     YGDGQQRRDWLYVDDHCQALEKILQYGKIGAIYNIAGHCHLPNLDVIRLILGEMEEQL
                     EPHDPCRKNLSQRLITRVADRPGHDRCYSLATARIKKEIDWQPRVALAEGVRATVSWY
                     LQNRSWLEQASNQSGESKEWQGMGNIISGGANSQTNEVNE"
     gene            21794..22558
                     /locus_tag="P5549_05290"
     CDS             21794..22558
                     /locus_tag="P5549_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRW12518.1"
                     /translation="MKGIILAGGKGSRLYPLTKVTNKHLLPVGSEPMIYNPIKQLVLA
                     GITDILVITSQEHMGEIVRLLGSGKQFGCDFTFKVQEEALGIAHALGLAESFAADDRM
                     VVILADNVTTSSIKPFVDEFARQDCGARVLLRRVDDPERYGIAALDELKIIEIEEKPA
                     QPKSAYAVIGIYMYDQEVFNIIRTIKPSDSGEYEITSVNNDYIQRNCLQYNILPGQWT
                     DAGTFESLHYANQIMLAAGNRISGYEEREQSHEDTF"
     gene            22521..>23485
                     /gene="rffA"
                     /locus_tag="P5549_05295"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             22521..>23485
                     /gene="rffA"
                     /locus_tag="P5549_05295"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRW12519.1"
                     /translation="MKKGNKAMKIPFNRVSLSGKEMVYLEDCIKRGLISGGGYYTYAA
                     RCRLEEYLYTSKVWLTTSGTSALEMAVLCLDLQPGDEVIMPSFTFVSTANAVLLRGAR
                     PVFAEIDPYTLNLDPEDAERKISSRTRAIIPVHYAGVACAMDDLLAIAAAHDIAVVED
                     AAHGIGAAYKGRPLGSIGLMGCYSFHGTKNIVSGEGGALLLNRGQQQLTERAEIIYEK
                     GTNRSQFMRGEVDRYTWQGIGSSFIMADLLAAFLYAQLERLEEITRARQQRFAIYQQA
                     FLPYQQQEKLQLPYVPEYAQSNGHLYHLLCPTQAKRDELLQSLRE"
BASE COUNT         6233 a         5321 c         6124 g         5807 t
ORIGIN      
        1 tcacacagtg atttgggcaa atgttcgtga atgcttctga tgaaattatc cgtttgaatg
       61 tttagactaa aataaaatta agtcaaaatg tctgtattta tgtttggatt taccaaaaat
      121 ggtcaacagt caaaagttaa aagtgtattc acaacaggta agaatgaaga agggggagac
      181 aaaatgagta acgaagaaaa acagaatgga ctatctcgca ggacatttat caaaggggcg
      241 gcagtgagct cggttatgct tggttcagct ggcgccctga tcggctgcgg caaggaagaa
      301 gaagcacccg caccgaaaga acccaataca gagagtgcca tcccaagttt tttgaaaaaa
      361 ccggatgcca ttccagatgc tgatatcaag caaaccgttg cggctgatgt cgttgtcatc
      421 ggtgccggta tgtcaggatt atgtgcggcc atggccgccg ctgaggaagg ggccaaggtt
      481 gttctgctgg aaaaaaccga ccgggtacat ttccgcggca acgattatgg cgccattggt
      541 tccaagatgc aaaagcaaat caacaatgaa atcaataaaa tggaagcagt ccaggaaatc
      601 atgcgctaca atgcttacaa agccaaccag cgggtagttt ccctgtgggc ggataacagt
      661 ggtatggtag ccgactggat catgggcaag gccgagggct atggctgcaa gcccaagccg
      721 gtacccctgg atgaaaccgt aaccccgggt acgaccataa aagggttccc caccctgagc
      781 ttcagaatgg acccaagtga agcagcgatt aatgatgctc ccaaaggaac cgatccttgg
      841 acagcttcaa tgcgttatgc gctgaaaaaa ggctgtgaag acgcaggtgt tcaattccat
      901 tacaagatgc cggctgttca gttggtgcgt caggacaagg gacgggttac tgccgtgatt
      961 gcgggagaaa aaggcaatta taccaaattt gaaggaaaaa aaggaatcat cgtttgttcc
     1021 ggtgactaca atgccaacaa ggaaatgcgt gaatattata ttccttccac caaatacatc
     1081 gatgctaata tgtatgagat ggtagacgga aaacaaaata ccggcgatgg tcagttgatg
     1141 gggatgtggg tcggtgctgc catcgatgaa ttcccgcatg cccccatgta tttcgacttt
     1201 gccgtaccgg gggctccgat tctggccgat gccttgatgc gtcagccctg gctgaatgtt
     1261 aatgtgcgcg gttttcgtta ttgcaacgaa gaactgcctt atgcctatct ctgcaacgcc
     1321 gaacgccagc agccggaaca ggccagatgg gcgatttggg atgccaaatg gccggaagaa
     1381 gcaccgtcct ttaaactggt cgcctgcaag gatatgcgca ccaacttcca caatcccgaa
     1441 agcgtaaagg cgtatgttga aaagggttat atcagaagtg ccgataccct ggaagccctg
     1501 gctgacatga tcaaggttcc caaggacaat ttcatgaaga ccgtggcccg ctacaacgaa
     1561 ctggccaagc tgggtgaaga cattgattac ggcaaacggg ctgcttgttt gaccacgatt
     1621 gagaaaccta ctttctatgc cgctccgttg ggtaccgcct tgctggttac cttgggtgga
     1681 ttgcaggtca atgaacagct gcaggtcttg gacgaagaaa agagtgtcat cccaggtctt
     1741 tatgccgccg gtaatgcctc cggcagctat tattccaatg actattgcgt aaacctccct
     1801 ggcaacagcc atggccgcgc gtatactttc ggttacctgg ccggcaagaa catcgtcaag
     1861 ctcggctaga agatattccc ccctccccat caataaccaa aacactgaga ggctgtcctg
     1921 cgggacagcc tctcagagca tatgaaggtt attgcctcta ttttactaat tgatattcct
     1981 tgattttgcg atacagagta gcccgactca ttttcagcaa atgggcggct tcagatatat
     2041 tgtgattgca ttgcagtaaa gcctctttaa tgaagatttt ttccacttcc ttcattgaca
     2101 gatgattgac cgggacatgg ggctttcttt gttttgcatg aaaagcggcc agagaggctt
     2161 tgatatcctc tggcaggtga ctcgtttcaa tcaagctgcc gttgctaaca tttagcgcat
     2221 aaaggacagc gttttccaat tgcctgacat taccgggcca ggcataattt aacagtgcaa
     2281 tcagcagttc atcgctgaat tccggcgcct taatcctttg cttttgggca attatactga
     2341 taaaataacg tgccagcagc acgatgtctt cacctcgctg acgcaaaggc gggatgttga
     2401 tttgtaaaac cttgagacga tataagagat ccttgcgaaa cagatctttt tccaccagat
     2461 catccagatc acggttggtg gctgtaatta gacggaaatc gacaggtaca tactgactgg
     2521 cgccaatccg catgaccctc ttttcatcca gcacccgcaa taaaaccggc tgcagttcca
     2581 gcggcatatc accgatttca tccagaaaca gggtgccttg gtcagccagc tcgattttgc
     2641 ccggacgacc ttgtttttca gcgccggtaa atgaaccgcc ggcatagcca aacaactcgc
     2701 tctcgatcag cgtgctggga atggcggcgc agttaaccgc tacgaaaggc ccattgggtc
     2761 ggctatcttt atggatggca tgggcaaaca tctctttgcc ggtgccgctt tcaccctgta
     2821 gcagaatatt ggcttccagc ccggaaaact tttgggacaa atcaatgcag tgcttgattc
     2881 cttccgagtc gccaataatt ttggtaaaat catagcgagc ctcaagcgta gtggcaggta
     2941 ccgcattttt tttggcttgc gcaaattttt taaacgttac catacagccc agatgctggc
     3001 cgaccgaatc ggcaatctgc tgggcagaga ggagatgtat cgtctggtcc caacgctcca
     3061 agtggatctc gctgtcatag accggtttgc ccccgtttaa tactgactct actgcggctt
     3121 gttgtcccaa aatggttttg tatggcatac cgattacatc atgttccagc agtttatcaa
     3181 aaaatccctg agcaatggca ttggcctggg tgatgatccc gcctttgttg atcatcaaaa
     3241 caccgatgtc cgtcgaattc atggtcacat ccagcagttc tttgttaaac agcagttgaa
     3301 tatctctttg gatggcccag gccatggata cggccagtcc gagagagtgg gcattttgct
     3361 gatgaaaact gggactgacg atggaaagag tgccggtcag attttgattg ccatcgaaaa
     3421 tcggcgcggc agagcagaaa atattatcat atttttcaca ataatgctcc ggcccgcaga
     3481 tctgcatggg aatctcatgg attagcgata tggcatgggc gcaggtgccg acggtggact
     3541 cattccagat ggaaccggga accagcttga agcgcttgtt ttgatcctcc aatttccggt
     3601 ttccctccaa tacgcgcaac atgcagcctt gttcatcact gagcaaaata atgcactgtg
     3661 aatcggccag catggaagcc aactggtgaa taaagggttc agatgagtcc aacagggtgg
     3721 ctttttcctg acagcgctgc tgtagcactt tgcccttcag ggcaggagcg taattgtatt
     3781 caaagacgtt tagaccattg tcaaatgaac gtatccagga atcaaccact tcgggcctta
     3841 ttaggtgaat ttcctgttta tctatgcgct tgttgattac gtcaagcttc agttttttaa
     3901 tttggttgat tagatcgatg tcttcggaat cgtaattatt gcccagtacc ccgcgattac
     3961 ggatcatagg acttaatgcg ttttccagtt tcatagcaaa tcctcccggt tagcttgcag
     4021 tatgatttta atagctgagc caaacgatgc ttcacgatgt ggtttaataa tctcataata
     4081 acacaatgct tgcaaatggt atttgagaaa tatgtattaa tatgagacgg agcttggaaa
     4141 gatagattgc aggaagccca tcaggcggct aaaactcggg tccatatcgg cttggcacaa
     4201 gaattgcgta ttaaataaat gagaaataca gggagggtgg tattatggta acagccatca
     4261 agagcagtga aatgttaatt gaaggggttc tggataatgg cctggtgttt atcggcgagt
     4321 atctggagag cattcttcgg cataccattc tcattgctga tcagaacgga aagatttact
     4381 ttccggaaag caatcagcat atgctcgatt tggatagctc ctttgttggt atttctgcgg
     4441 agatggtgac gagggagtcc ttctactgtg aggaaagcca tcatttgtat tactgtgtgg
     4501 agaccaaaca tcagcgtgcc tatgtgattg tgaaagactt gaacacggag tgggtgccac
     4561 gtattctgga aatcctgaga gagagcagac tgcccatcaa gtgttatttc tcaaaaataa
     4621 acaaaagcaa ggaaatgttt actgcagaat taaaggatta tttgtttggt gccggtcagg
     4681 cagatattcg ggatattctg tatttatgtg atagcgaact ggatttacag cgaccttatt
     4741 ttgccttgct gctgtgtgca gaagagggtt gtttaaaaac agatccggaa atcattcatt
     4801 cttatgtctg cgaacacctt cgtcaggaaa aatgtaaaac catctcggtt tatcatgacc
     4861 atcagtgggt ttttatgatt cctgcgcaga taaaagacga ttgccgaatc aatgaaatgg
     4921 aaaatgagtt tgatttaaat gctttaaaaa gagctattga aaataagttt aaaatttctg
     4981 cttcgatggg tgtcggacaa gtatatgcgc tgtccagtct ccgtaagagt tttcgtgaag
     5041 cacgcatgac catgatcctc aatcatttga ccggattgca aagttttatt cagaagttct
     5101 ctgatttggg gatttacaga ccgatttttt cccaggaaat gaatcatatt tataaatact
     5161 gcagttgtac actgggaaaa ctgatggaac atgactgtaa atatgatggt gaactgctgc
     5221 caactttacg aaaactgctc gatacctgtg gcaacaacaa agccactgcc gatcagttgt
     5281 ttattcatgt caataccctc tactatcgca tcaataaaat tgaacagatc ctggacatgg
     5341 atctttccaa aatgcatgcc cgggcggaat tatatactgc gatcaaagta tgggatacgc
     5401 tgcaggtttt gaatggcaag gaggaaatga cagatactct cccggccata ctggcaatgc
     5461 aggcataagc tgtccttgct gacagacagg aaaacgtttt ctggtgttag cgctgtcctt
     5521 catcaacccc tcgccgtaaa tcaatcctta tagacaatat gagctgtttt gccggtgcaa
     5581 aatagctatt tttttataaa tcagtataat ataaaaggca aggaggttat cacctatgaa
     5641 gcatgatcag gccccgccga ggggctatca tatattgact tatggctgtc agatgaatag
     5701 ccgcgattcg gagattatca gtggtttgct ggagcaggca gggtactgcg aggttccttt
     5761 ggaagcggcg gatctggttg tcttcaacac ctgtagtgtg cgtcattcgg cggagaacaa
     5821 ggtgtatggc aagctgggtg aagtcaataa aatcaagcgc agcaaacccg gcctgcggat
     5881 cgcatttgga ggttgcatgg cacaattgcc ggaagtcgtt aagcggctga agcgccaagg
     5941 ggtggatgtt gtatttggga cccataattt gcaccagttg ccgatattgc tggaaagaat
     6001 cgatgccggt gaaaaatccg ttatcgaagt ctgggagcag catggagaaa ttattgaaga
     6061 tttaccttcc aagcgaaaaa aaagtgtcag tgcctttgtc aatatcatgt atggctgcaa
     6121 taatttttgc agttattgca tcgtcccgta tacccggggt cgggaacgca gccgcaaggc
     6181 ccgggatatt atttgggaat tggagaaaat ggtcggcgag ggcgtcaaag aagttaccct
     6241 gctgggacaa aatgtgaatt cctacgggca aggtggcgac gagagcttgg attttgccag
     6301 tctgctgcaa atggctgaca aagttgaggg tttgcagcgg attcggttta tgacctccca
     6361 tcccaaggat atgtcggatg ccttaatcga tacgattgct gctggagaaa aactttgtga
     6421 acatattcat gtgccgctgc aggcgggcag caaccaggta ttgaaacgaa tgaaccgagg
     6481 ctatacgcgt gaatcctatc tggaattgat caacaaaatg cgggcgcgta ttccgggggt
     6541 ggccattact tcagatttga tcgtgggttt tcccggtgag agtgaagcgg attttgatga
     6601 taccttggaa atggttgagc gggttcgttt tgacgctgcc tttaccttta tgtattcagt
     6661 tcgaaccggt acacgggccg cttctttcaa tgatcaaatt gaattggcgg tcaagcagaa
     6721 ccgtttgctg cggctcaacc agcagcaata tgatatcgct ctgcaaaaaa atcaggccct
     6781 ggaaggcagg gtaatggaaa tcctggtgga tggccccagc aagaccaatc ctgatcgact
     6841 gacgggcaga acccggacca atctgattgt aatattttct ggtccaaatg atttaattgg
     6901 ccaactcatt aatgttaaaa taacaacagg caaaacattt tctgtttttg gtgaactacc
     6961 ggcggaaacc agctgaacaa tgttccgccg ctccaatgaa aagaatcaga ttcgcccatc
     7021 aattttgagg aggagtaata aaaatgacca ctgaagaaat catcaaaatg gcctacgagc
     7081 tggggaatgc gattgcgcag tccaaagaag tcgatgcgtt acgggaaatg caggttaaac
     7141 tcggtgatga tgctgatgca tttggactga tcatgcgtta tcaggaagcg caaacccaga
     7201 tggagaataa attgcaggac ggcttgcagg taactgcggc cgaggaaaaa aatattgaga
     7261 taatggaaga agagattaac aacaatccgc tgatccagga gctgatcaag gtgcaggaga
     7321 aattcgacag tctcatgcag ggggtctatt ttgccatgaa tcaagccatc tccggcagtg
     7381 acacttgttc atccgggtgt gattcctgcg gcggcggctg cggtgtcatg taaggaataa
     7441 tacgcttaca atctgcatgc tggaaggctt gatcatttga tccaagcctt ttcttgtaac
     7501 gagagggagg gttggtttgg ccaaattcac acccatgatt gaacaatatc tcgaagtcaa
     7561 ggccaatcat caggatgcga tcttgttctt ccgcttgggt gatttctatg aaatgttctt
     7621 tgaagatgcg gtgctggcag cacgtgagct ggagatcgtc ctgacctccc gtgacggcgg
     7681 gcagcaaaag gttcccatgt gcgggatccc ttaccatgcg gtcaacaact atctgccccg
     7741 gctgatcagc cgaggctata aagtcgccat ctgcgaacag gttgaagatc ccaaacaagc
     7801 cagcgggatc gtcaaacggg aagtcattcg cattatcagt cccgggacga ttttggaaga
     7861 ttttatgctg gacgagggtg ccaacaatta tctggccgcc gtcgacatct atgaaaaaac
     7921 gattggtttt gcttatattg atatctccac cggcgagttt caattcaccg aaatcaacgg
     7981 accccatgcc cgtcaagatc tgtatgctga gttgcacaga gtggcaccgg cagagtgttt
     8041 gctgtccgct cgtctgactc gcaattcctt atgggaagaa caaccgttgg gcaatgattt
     8101 tctcttttcc cgttttcccg aggatgacct ggatttggat caaagccggg cagtaatggc
     8161 ggatcatttc ggatcggcat ggctggatga agggagcata tccgcttgtc atgcgggctg
     8221 cttggcagcg ggcatgatca tcaggttcct ttacaacacc caaaaaagtc gtctgttaca
     8281 tatagacggc ctgcagttct atcaaagcag tgattacatg gaactggata gtttcacgcg
     8341 ccgcaatctg gagctgacct ccaccatacg ccaagggcgc aaggaaggct ccttgctgga
     8401 tattattgat caatgccgca cgcccatggg caaacgcaac ttgcgccgct ggctggaaca
     8461 gccgttgatg ggcatggaag ccattaacca gcgcttggac ggagtcgaag aactcgcagc
     8521 ggatatcggc ctgcgtcaat ccgggcggga tgcggtgaac aagatgtatg acctggagag
     8581 gttgtctgga aaactaggca gtccgctgct taatccccga gatttgctgg cgctcaaaca
     8641 ttccctggcg ttgctgccag cgatcaaggg gcttctgtgc aactgtaaca gtcaaatcct
     8701 cagagcgatt gccgagctgg agccgttggc cgaagtacat gaaatcatcc actctgccat
     8761 cgatgatgaa gcgcctctga cggtgaggga aggcggtatc atcaaaagcg gataccatgc
     8821 ggagattgat gaactgaagt ccctgggggt ccagggcaag aactatctcc tggaatatga
     8881 gaaccgcgaa aaattacgca ccgggatcaa gcatctgaaa gtcagctaca acaaagtctt
     8941 cggttattat attgaagtca gcaagtcgaa tctgaatctg gttccagctg attacatccg
     9001 caaacaaacg ctggtcaaca ccgagcgctt tatcactgaa gaattgaaaa attacgaaga
     9061 caagatcctg ggcgcccatg ataagctgtg tgtgttggaa tatgattgtt ttatcgaaat
     9121 caaacataat ttggaaccgt ttattcccgt ccttcaagcc agcgcaaagg cagtggcgga
     9181 ctttgacagt ctgctctccc tggcccaggt ggcttttctg aatgattatg ttcgcccccg
     9241 gttgtctcgt gacggccgac tggaaatcaa agccgggcgc catccggtgg tggaaaaatc
     9301 actgcttgat acccgctttg ttcccaatga catcatgctg gacaatcgcg acaaccgctt
     9361 tgccattatc acgggtccca atatgggcgg caaaagcacc tatatgcgcc aggctgccct
     9421 gatcaacatc atggcccaga tgggcagttt tgttcctgcc agtgaagccc gcatcagtat
     9481 cgtcgaccgg gtctttacca gagtcggagc agccgacgat ctttccgccg gacagagcac
     9541 gttcatggtg gaaatggtag aactggcaca tattttaaac catgccaccg ccaacagctt
     9601 gatcattctc gatgaaatag gccgcggcac cagtacttat gacggactga gcattgccca
     9661 ggcggtcagc gaatacatcc atgacaaagt caaggcccgt accttgttcg ctacccatta
     9721 tcatgaactg acccggctgg cggaagaaaa aaaggggatt atcaatcttt cggtttccgt
     9781 catggaatcg gtggacaccg ttaccttctt aaaaaaagtc ctgccaggca aggccgacaa
     9841 aagttacggt atccatgtcg ctcaaatggc tggtctgccg cgaacggtaa cggatcgagc
     9901 ctatgaagtc ctggccgcgc tggagagcaa tgaagccctg ccccgccatc ctgaggtcca
     9961 gcaaataagt ctgtttgcgg aaggacccaa tgaaatcctg gaagaactgg atcatctcga
    10021 tttggacaat ctcagtgccc gggaagcctt gatgatcctt taccattgga aagaaaaacg
    10081 cagggggtga gaatatgttg atcggaattg atgtaggcgg gacctttaca gacggagtgc
    10141 tgttcgacaa tcagcagtcc cgcgtagtaa agacggttaa agtgccgacc cgcaacgaac
    10201 agcttcaatc cagtctgttg gcagtattgg atgccttgct gccaggtcag gagaggggag
    10261 gaatacaacg gattgttttg agtacgacgc tggtgaccaa cctgctggct accaataccg
    10321 ccgagccacc ggcgctgatc ctgatccccg gaccgggtct gccctttgag tatctgctga
    10381 cctttcccca tagttatatt gtcaaaggca gtattgactt tcgcggacgg gaaacggaaa
    10441 agctggatcg gcaggaaatc gaaggcgtaa tcgaaacgat tcgcgccgcc ggtttgcata
    10501 aaattgctgt ggttggcaag ttctccaatc gcaatccgca acatgagcag agcgtgcggc
    10561 aaatcatcat ggacaaattc ccggacgccg aggtgggaat tggttcggaa ttggccggac
    10621 agctcaattt catgcgccgt atcacgactt cctactatgc gctgatgaca cgccagcgct
    10681 ggacatcgtt tgcccttgag attgaaaagg cgatccggga acgcggactg gacaaagttc
    10741 cactggatat tctcaaggcg gatggcggaa ccatgccctt gcgtaatgct ttacaatacc
    10801 catgtgaaac catcctttcc ggtccggctg ccagcaccat gggcgccaaa gcgattaccg
    10861 ggcagaatac caatgcagtc gtacttgaca tcggcgggac caccaccgac atttcactgc
    10921 tgttggacgg acaaccccta tatgcttcaa gaggcgcctg catcaatggg aaatataccc
    10981 acattaaggc catatccacc cgctccctgc cgctgggagg cgacagtcct gtttgttttg
    11041 aaccgcaaaa aggggtatat attcatccga tcagaagggg accggctgtc tgttttggcg
    11101 gcgaatgtcc taccgttacc gatatcttca actgtaaata tgatttaaat attggtgatg
    11161 ccggtcaatc aagccaatcg ctgcagcgtc tgcagaagga ggcaggctta accttgcctg
    11221 aactgctgga gttggtggag catgatgtaa tgcagcagtt gatggaggct ttgcaaaata
    11281 tctttatgga atgggaaaat gaaccggcct atcgggtctg ggaagtggtg cataaccgca
    11341 agtttaaagt caaacgcatc atcggaattg gcgccgcggc ctctgccatt gttccgatgc
    11401 tggccgccat gaacaaggtc aacagcttta ttcatgaact gtctcccatc gccaatgccc
    11461 tgggtgccgc cctggccagg ccgaccctgg cttttaacat gcatattgat acccaaaagg
    11521 gaagctattt taccgattat gatggagtcg ggggtgatct cccccgggga caaagcatgc
    11581 aaatggtgga tgcccacaaa ctggcccggg aagtgatgca aaaactggcg gaacaaagag
    11641 ggctgggaga atatgcggaa gaagcagaat ttgtgcgtga agaacaattc aacatgatcc
    11701 gggagtggga tcgaatcggc aaaattttcg aattggggat gcagatcaaa cccgggttta
    11761 ttaaagaatt tgaggagggg tcattatgaa aaataccggt gttatctttt tcccggcgtt
    11821 tgactgggcg atctccccga cccatcccga acgcgaggaa agactgctat acacccgcga
    11881 tcagatcttt gaagaaggta tcatggattt gcccaatatc catgaattca aaccccgtct
    11941 ggcggacaaa aaagacattg cccgagttca tttttgtgtt cccaacgtgg aagcccaaat
    12001 caccgatgct catttggtat ctgccggttc tgccctggtg attgccgacg cggtcttgcg
    12061 gggtgaagtc gacaacggct tttccattgc ccgtccgccg ggtcatcatg ccatgacggt
    12121 ggcacacggc aatcgcggtt tctgcaatat caacaatgaa gccatcatga tcgaatatat
    12181 tcgcaagcaa tacggtttac gcaaaattgc ggtggtagac acagacgttc atcacggcga
    12241 cggcagtcag gaaatctttt atgatgaccc ggatattttg tttatttcct tccatcagga
    12301 tggacgcacc ttatatccgg gcaccggttt tgtatatgaa ctgggcggac cgatggcctg
    12361 gggaagcacc atcaatatcc cgctgcctcc ccagaccacc gatgaaggca ttctgcgggt
    12421 gctggacaaa ctggttttgc ccgtcttgga agaatttgcg ccggaactaa tcgtcaactc
    12481 agccggacag gacaatcatt atacggatcc cttggccaat atgcgcttta ccgctcgcgg
    12541 ctatgccgaa ctcaaccgtc gtctggcccc cgatattgct gtgctcgaag gaggatactc
    12601 ggtcgagacc gcgcttcctt atgtcaatat ggcattgctg atggccatgg ccgggattga
    12661 ttacgccaat ctgcacgaac cggattaccg accggaagca ttcaaggaaa gtgcgaaaaa
    12721 tttggagtat attgagcaga tcatctccca tcagctcttt aatttccgca gccggcagca
    12781 gaagatagaa gagaaccgca aaaaatatgg tcatgaatat ggggaacatc agcagattta
    12841 ttatgatacg gaagggatca gcgaagatca agcccgccgt ctgcgaatat gtccggcctg
    12901 tcccggttgg cggcgcattg attcctcggc ccgccatcgt gacggtaaaa cgtatcatgt
    12961 tttctgtatc agcatcccca cttttgcttg tccagagtgc cagaaggaag cttttaacgc
    13021 ttaccaggat atggccatgt acaatcacca ttatgattat atctatttgc aggatcgggc
    13081 caaagacaga tatattgctc ttaacgtggc caacgggcgg gaagaggtct acgaataaat
    13141 gagtatcaaa ctgctggatg ataatctcat taatcagatt gccgccggtg aagtcataga
    13201 gagaccgtcc tcggttgtga aggaactggt tgaaaacgcc atcgatgccg gcagcacgag
    13261 gattcaagtg aagatttccg gaggcggact ggaagtcatc gaggtggaag acaacggctg
    13321 cggcattccg gagagcgata tcgaactggc gctgcagcgt catgctacca gtaaaattgc
    13381 cttgccggat gatttgttca atatcagcac catgggcttt cgcggggaag cactgcccag
    13441 catcgcttcc atcgcccgac tggaggtata cagccggcaa aaggatggca tcggtgcgca
    13501 attgcagttg gacggcggga ccatcatcag ccggcagccc tatgccggta ccgagggaac
    13561 acgcatcgtc gtgcgggaat tgttttataa caccccggta aggaaaaagt tcatcaagtc
    13621 gacggttgcg gagcagaccc atatttatga aaccatcagt cgtctggcgc tgtcccgccc
    13681 ggatatttcc ttcctgtttg ccagcgaaaa aaagatgtat tttaagactc cgggcagcgg
    13741 cgtgctgcgc gatgcgctga ttgccatcaa cggcaaggat tatgccgacc actttattga
    13801 ttttcatgtc aggggtgaaa aatacagcgt ggccggattg ctgtccaaac ctgaatttcg
    13861 gaggcagaac cgcaaaaacc aaatcttttt tgtcaaccgg cgcctgatca aaagccccat
    13921 gctgaaccgg gcagtggatg aaggttatcg gggactgctt ctggcccgtg aatatccggg
    13981 cgtcattctc tatttaacgg cccattgcca ggaggttgat atcaatgtcc acccgcagaa
    14041 gagtgaagtc agattccggg acgaaagtga aatctttcga gtcgtcagtc gtgccatcaa
    14101 ggacgcggtc aacggactgg cctacaccat gggggaggct gcctgggcag agagaatcgc
    14161 agaacccgaa gcaatcttca aaccggaggt caatcccgga agaaacggag catacattcc
    14221 ccctccgcgc gcgttcgagc agtcatttac ttttgtatat ccccgggaca atcaagcggc
    14281 ggcagggagt ccggaccgat tggcagatga attgccggct caaagtcctt tccgcttaat
    14341 tgggcagtgt ttcaacgcgt atctcttggt ggaaacagaa gggagactgt ggttgattga
    14401 tcagcatgcc gcccatgaac gcatcatgta caaccaactg cgccggcaga ttgctgcggc
    14461 cccggtcgcc acgcaggcat tggcttttcc gctgggactg gatctttctg cggcccaaat
    14521 cgatatgctc gaagccaacc gagctgtttt tgaaagggtc ggtctgtact atgatattct
    14581 cgggccggac agtattgcca tccgctcagc gccgccggcg ctgcatggtc aggaaagtga
    14641 attgctgctg gagattttgg agatgatcgc agcagagcag tgtccggata ttcatgacga
    14701 agcaatttgc atgatggcct gcaagcaagc cattaaagcc aatcagttgt taagccgggc
    14761 ggaaatggag aaactggtga gtgagctcct gcagcaagag aattatctgc attgtccgca
    14821 cgggcgaccg tctttcatgg agatcagccg cagcgacctc gaccgccgct ttaaacgata
    14881 acacgcaagt tggggacggt tcttgacttg cgtaatactg ctgattttgt ctgagtttta
    14941 tgcattagtt tcgccagggg cccatctttt gactggctaa agttgaatta ctagcgctcg
    15001 cttgtgacct cggagtcttg atactaattc gaaagtctcg tttttgggta acgaatattt
    15061 ttgttaattt tgtgagctat ctttcgtcgt aacgttgcgt cgcacggctg ccgccttacc
    15121 cccggcgctt ttccttaata gtgttcttaa tccgaagggt tagaacacat ataaaatacc
    15181 cggagggtgc cactaaaaca taaacctctg cgggggactt tcaaggaaag gtataatcta
    15241 tggaattaat cgttacaacc acccagagga tcaaagagaa ggaagctgat ttggaggaat
    15301 tcctggcgga gagcgggctg cagtttgtgc ctcgtgaccg cagcctggaa aagctcaggg
    15361 aaaaatacca ggctgcggga gttgttgtct ggggtgctga ggtcccgata ttgtattttg
    15421 aaaatgaaaa actgtatttt catcccagta tggctaaaaa ccgcattgct ttttatcgca
    15481 aacaacagcg tccggatatc atgatcgaag cggccgggct tgaaagagga gacagttttt
    15541 tagactgcac gctggggcta ggggctgatg cgattgtggc ttcttatttt tccggtcacg
    15601 gccgggtcac cggattggaa catcagccgg agatcgcttg ggtgattcgc tggggaatga
    15661 agcgctattg caacgagatg atctggctgc aggaagcgat ccggcgggtt gaggtggttg
    15721 cggccgatca taaggagtat ctgcctggtc tgccagactg gtcttatgat atcgtttatt
    15781 ttgatccaat gtttccccat ccaattttca agagccagcc aatctcgccg ctgcgcaagc
    15841 tggccgacca tgaccgcctg gaagcggcga gcatcaagca ggcctgccgg gtggcacgca
    15901 agcgggtggt tatgaaggat ctggcggaag gcgaggaatt ggaacggttg ggttttcaaa
    15961 aaatcagccg cagcaagtac aacaagctcg cctatggggt tatctatgtt tagagtagat
    16021 aaccccgctg gaaatatgta tatgaatggg tgacgaaatt tgaaattcta tgccttggtt
    16081 ggcccgaccg cggtgggaaa aacagcatta tcaatcgcgc tggcgcaaca gatcgatgcc
    16141 gagatcattt cctgtgattc catgcaggtt tatcgtggca tggatatcgg cactgccaag
    16201 gccaccgtgg aagaaagaaa aattgtcctg catcatatga ttgacattgt tgatccggac
    16261 gtttctttta ctgttgccga ttaccagcgt atggcccgtg aagaaatcct gcggcttaat
    16321 caaaacggaa aaccagcttt gctggtcggt gggaccgggc tttactatca ggcgctggtg
    16381 gatgattttc actttttccc gctggagtcg cgtgatcagg tacggcaaaa atgggagacg
    16441 gtttgtcagg aaaaagggca ggcgttttta tttcaggaat tgcggcgggt cgacccggta
    16501 tatgctgaaa aggttggcgc caatgaccgc aaacgtatca ttcgtgcatt ggaagtatgg
    16561 gaattaaccg gagagccctt ttcctgtttg cagaggaaaa accagcacag ccatcaagtg
    16621 caagtcgttg gcttgtatgt ggaacgggaa gtgctttatc accgcatcga agcccgcgtg
    16681 gacaagatga tagaggaagg attggttgag gaagtgctca ggcttcgcac ccaaggctat
    16741 gattccagtt tgaagtcaat gaattcgctg ggctacaaac aggtcaatgc gtatttggac
    16801 gggatgctca attttcagga tatgctgtat gagatcaaac gcgaaacccg tcactttgcc
    16861 aaaagacagt tgacctggtt ccgcaaggac cagcgtattg tctggtatga tgccgggcag
    16921 gaacagacgg tcgatttgct ggcaaaaatc cgtaatggct tggcaggaca aaaccggctg
    16981 gtgtagaata gtctacataa cactaccaag cgatgttcac aaatgaaagg gagggcctga
    17041 cattgatgaa aaaccaaatg aatttacaag acgcatatct taaccagatt cgcaaggata
    17101 aaatactggt taccatatac atgatcaatg gttttcaaat caagggagtg gttaaaggtt
    17161 ttgacaactt tacggtgatt gtggagttgg acggacgcca gcaaatggtg tacaaacacg
    17221 ccatctccac catcagtccc ttcaaaccga ttggcgtcat taactttgaa aacaagaacg
    17281 aagaagaagc ttaaactgat gacaaagtaa aagacggaaa gggaagggca ttttgaacac
    17341 cattgaaatc ataatgggta ttattttatt gctgattatt ctgatgattg gcgacataaa
    17401 aaagaaggtt atcgccttga tcatttttgc cgccgtcgtg gccgaaaaac tgctgcactg
    17461 agccaaatga aaaacatatt ttttaaaaaa atcggggtct aaaccccgat tttattttgg
    17521 cggcaaaaaa atggttgaca ttattgaaat aggcataata gtataagcat acgcttatac
    17581 taaaatattt taataatatc ataatcacaa gcagcaaagg agaagggact gtggagatat
    17641 ttcaatggtt gaataaccag ttgcttaaaa tggaatggct gtcagatctc atcaggaaac
    17701 tggtggagaa tggactgaat ctggatatga gtacccgtct gggtggcagc attcattttt
    17761 ttatttacga tgtaatcaag atttttattc tgctggcggt attgatcttt acgatttcct
    17821 atatccagag ttttttcccg cctgagagaa cccgggccat cgttggtaaa tacaagggct
    17881 tctgggccaa tctctttggc gcgttgctgg gtaccgttac cccgttttgt tcctgttcgt
    17941 ccattccctt atttattggt tttaccagcg ccggattgcc gattggggtg actttttctt
    18001 ttttggtatc atctcctctg gtagatctgg cttcggtgct gctgctggcc agcattttta
    18061 attggaagat cgccattgcc tatgtactgg ccgggctggt gctggcagtc gcttccggaa
    18121 cgctgatcag ccggttgaaa ctggaaagtt atgtacagcc ttttgtgtac ggcaatgaat
    18181 cgtttgaata tgccccaatc aagatgacca cacaggagcg tatttacttt gccaaggcac
    18241 aggtcagaga tattgtcggc cgggtttggc tgtacattct gatcggggta gccattggcg
    18301 cagccattca caattggatt ccccagaata ttatctccgc agtccttggc caggacaaat
    18361 ggtattcagt cttggttgca accgcggccg gcatccccat gtatgccgat atctttggca
    18421 ctctgccgat tgctgaggca ctggtgtaca aaggtgtggg gattggtact gtattgtctt
    18481 ttatgatggg ggttacggcg ctgtcactgc cttccctgat tatgctctcc caagtggtta
    18541 aaccccgact gctggcaatt ttcttcacat tggttacgct ggggattatt gtgattggct
    18601 acacatttaa tgccctgggt cattttttga tctgaccgca tacggcagat gacgtgcctg
    18661 gattagcagt ccagttaaac tgatcaatta aggaaatcat tatatgatat aaggagagga
    18721 aaaacatgtt tgaaaccgta ttcaaggcgt tgggagaacc tacgcgtctg aaaatcatta
    18781 aattactggc agggcgggat ctttgtgttt gtgatttgga ggaggtcatg cagatcagcc
    18841 agccgcgaat ctctcagcat ttaaaagtat taaaatatgc ggggctggtt aatgaacgca
    18901 aagaaggcca gcgtaatata tgcagcttaa accgcgatca attaaaccgt atttgggatg
    18961 gttttcagcg ctatatcgaa caaccgcttg aaaatatggc agagttcgct gatgaatgcg
    19021 cccgtatgcc tgcagagatg ggagattatt gtgccaacaa aaaaggcgat taaaaattaa
    19081 caagtattag ggaggaatgg ttttcataaa caaaatgaaa tactgtgatt acaagatatt
    19141 gaaagaaaat gaagtttatt aagggaggtc caacaaatga aaatcgaagt attgggagcc
    19201 ggatgtgccc ggtgtaatca gatggaaaaa gacgtgatca atgcactggc tgaactggat
    19261 attgccgctg atgtgaccaa agtaaaagac atttccaaaa taatggctta caaggtcatg
    19321 tctacgccag ctctggtaat cgacgggcag gtgaagattg ccggccgggt gccgagcaaa
    19381 aaggagttgc acagcttact gaaggatgca cagaaataat ttgaccaacc ggatgtagtc
    19441 ttgatgctca ccggcaacca tgaaagccgc atgccatgcg cttttctttt aaggtgtggc
    19501 atttttctta tcgggtcata agcaaatcaa ataaatgata ccctcaattc actctataat
    19561 gaaatagaaa cacaagtatc agatcagggg tcattgcgtg accgacgata gcgatactgg
    19621 gttttacgtc aagcaagata gagtaagggg gagttaatga atgaagaaca ggaaaatgaa
    19681 atggttgccg cttttattgg tgatggccct gctggcaggt ttggttgccg gctgcagcag
    19741 cgccaaggag caggaacccg cagccaaccc gccggtacaa caggaagtca aagttgtcag
    19801 cattgaagaa gttgcagcca aatattttgc ggaaaatccg gaaagcaacc gggtaatgcc
    19861 tgaagctgcc ctgaaagaaa gacttgatgc caatgacagc cagttgtttg tggttgacat
    19921 ccgttctgcg gaggactatg cagccggtca catcaaaggg gccgtcaatg caccttttgg
    19981 caaaatgcat gagttccttg acaaactgcc ggcggacaaa gagattgtgc tctattgcaa
    20041 gagtggacaa acagccggac agactgtagc tatcgtcaac atgtatggat acaaggcagt
    20101 ttctctgaat ctcgggttcg acaacggctg ggtgaaaaag aacaacttcc cggtagatac
    20161 aactccggtc gagtttccgg ccaacctgac cccggccaaa ccagatgcca acattgccaa
    20221 aatcttaaaa gatttctatg tcaacatgcc ggccaatacc aacatgattg caccggctga
    20281 tctgctggcc aaaattgaca gcggggaaga catccaaatc gttgatctgc gcaaagctga
    20341 agactttgcc aaaggccatg tcaagaacgc catcaacatt ccgttcggac agctgggaca
    20401 gaattacagc aaaatctcca cggaaaaacc ggtctttgtc tactgctaca gcgggcagac
    20461 cgccggacag tcagtggctg ttctgaaagt actggggtac gatgcattta ccctcaaggg
    20521 cgggtacaat ttcggctgga cgacggaagg catgccggtc atgcaagaag ctgcgtaaat
    20581 aatagaaaac tttgttataa ttgtataaac aacgttacag gagggaaaga ttttccctcc
    20641 tgcttgttta tcagctgatt gtcagtttaa gaatgagaga gaaaagacag gtggtgaagg
    20701 tgctggatac ctatttgatt acgggtgggg caggctttat tggttcccat ttgatcgatc
    20761 tgctactgag caaaaagcaa ccccaagtaa gggtaatcaa cctggacaac ctgagttatg
    20821 ccgggactcg tccttatccc gaaaatcatg tcaatgaaaa caattaccgc tttatcaagg
    20881 cagatatctg tgacagtgcg gctgtcgaag agatatttgc ccgttataag cccgtcaagg
    20941 tcatccacct ggcggctgaa tcccatgtgg accgcagcat tattgaccca ctcatatttg
    21001 ttcgtaccaa tgtgacagga acccagatat tgttggaggc agcccgtcgg tttgcaaccg
    21061 aattgttcct gcaggtttcc acagatgaag tatacggtgc ctccaccctg gaccatccct
    21121 gcagtgagac cagtccccta aatcccagca gtccctacgc agcttcaaag gctgcggcag
    21181 atttattggt ccaagcctat cagcgcacct atcagctgcc ggtgattatc agccgctgta
    21241 ccaataatta cggcccccga cagtttcctg agaaacttat cccgttggtc attgctcatg
    21301 ctttgcatgg aaaaccaata cccgtttatg gagacggaca gcaaaggaga gattggctct
    21361 atgttgatga tcattgccag gcgcttgaga aaatattaca atatggaaaa atcggggcaa
    21421 tctataatat cgccggccat tgccatctgc ccaatctcga tgtcattcgc ctgattctcg
    21481 gggaaatgga agagcaacta gaaccgcatg acccctgccg aaagaacttg agccagcgct
    21541 tgattacccg ggttgcagac cgtcccggcc atgatcgctg ctacagtctt gccacggccc
    21601 ggattaaaaa ggagattgac tggcagccgc gggttgcttt ggcagaaggc gttcgagcga
    21661 ctgtatcctg gtatttgcaa aaccggtcat ggctcgaaca ggccagcaat caatccgggg
    21721 aaagcaagga atggcaagga atgggaaaca tcatttccgg gggagccaac agtcaaacaa
    21781 atgaggtgaa cgaatgaaag ggattatttt agccggaggc aagggcagcc ggttgtatcc
    21841 gttaaccaag gtgaccaaca agcatctgct gccggtcggg agtgagccga tgatttacaa
    21901 tcccatcaag caactggtgt tggccggcat taccgatata ttggtgatta ccagtcagga
    21961 gcacatgggg gaaatcgtca ggttgttggg cagcggcaag caattcggct gtgactttac
    22021 attcaaggtg caggaggaag cgctgggaat cgcccatgcc ctgggcttgg ccgaatcatt
    22081 tgccgcggat gatcgtatgg tggtgattct ggctgacaat gtgactacca gcagtatcaa
    22141 accgtttgtg gacgagtttg cccgccagga ttgcggcgct cgggtattgc taagacgggt
    22201 ggatgatcct gaacggtacg ggatcgctgc tctggatgaa ctgaaaatca tcgaaattga
    22261 agaaaagccc gcccagccca agagtgctta tgcggtgatt ggaatttaca tgtatgatca
    22321 agaggttttt aatattatcc gtaccatcaa gccatctgac tctggcgaat atgagatcac
    22381 ttccgtcaac aatgattaca tccagagaaa ttgtctgcaa tataatattc ttcccggtca
    22441 atggacagat gcggggacgt tcgaatcatt gcattatgcc aatcaaatca tgcttgcggc
    22501 cggcaaccgg atcagcggat atgaagaaag ggaacaaagc catgaagata ccttttaacc
    22561 gggttagcct ttccggaaag gaaatggttt atctggagga ttgtatcaaa cggggtttga
    22621 taagtggcgg cggatattat acctatgcag cccggtgccg gctggaagaa tacctgtaca
    22681 cgtccaaggt ttggctgacc acatcaggaa ccagcgcgct ggagatggcc gtgctttgtc
    22741 ttgatctgca gcccggggat gaagtcatca tgccctcctt tacctttgtt tcgacggcca
    22801 acgcggtcct gctcagaggt gcccggccgg tttttgcgga aattgatcct tatacgctga
    22861 accttgaccc ggaggatgca gaacgaaaaa tatcttcccg cacccgggct ataataccgg
    22921 ttcattatgc cggagttgcc tgtgcaatgg acgatctgct ggccattgcc gctgcacacg
    22981 atatcgctgt ggtggaagat gcggcccatg gcatcggcgc cgcttacaaa ggcagaccgc
    23041 tggggagtat cggattaatg ggctgttaca gttttcatgg taccaagaac attgtcagcg
    23101 gtgaaggcgg ggctctattg ctgaatcgtg ggcagcaaca attgacggag cgcgcggaaa
    23161 ttatttatga aaaaggtacc aaccgcagtc agtttatgcg gggggaagtt gaccgctaca
    23221 cctggcaggg gattggttca agctttatta tggctgatct gctggcggct tttttatatg
    23281 cccaactgga aagactggag gaaatcaccc gggctcgaca gcagcgcttc gccatctatc
    23341 agcaagcctt tctgccatac cagcagcagg aaaagctaca gcttccttat gtgcctgaat
    23401 atgcccaatc caatggtcat ttatatcatc tgctctgtcc cacccaggca aagcgggatg
    23461 agttattgca aagtctgcgg gagca
//
LOCUS       DAOTHZ010000031        23231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2597_length_23231_cov_7.974025, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000031 DAOTHZ010000000
VERSION     DAOTHZ010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 23231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23231
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_23231_cov_7.974025"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..973)
                     /locus_tag="P5549_05300"
     CDS             complement(350..973)
                     /locus_tag="P5549_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12520.1"
                     /translation="MNEVIENNLGLSGIIKRWKETEVGNYVHPVCQYKNVEAKTCLFC
                     YGMIPFPVRNLFTDTDWYVWIRPRKELFKVSEVKFRSAEIGVTDSYYQYNIRDWVFGS
                     KDRGSFGHSDSLQKEADKFLRRDVLHGLEILGADTSEGVDTIVVDIYQGNRTGGDESD
                     SLTFYDIEFVSNVVFPGFFSIGGGSDVGFGVISLKKNTQEYSQSGGR"
     gene            complement(1045..1479)
                     /gene="cas2"
                     /locus_tag="P5549_05305"
     CDS             complement(1045..1479)
                     /gene="cas2"
                     /locus_tag="P5549_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRW12521.1"
                     /translation="MKLRKAGISGLRGLRQGEGVDELPSLPERIDKVLGVLNTQNNNP
                     RQMIYFIMYDIESNKVRTLVAKYLIRKGCTRVQKSIFIASTERPVFDEIRKDLKEVQE
                     CYDNSDSIILVPVSTDEIRAMKVIGQNIDFDLALKNKNTLFI"
     gene            complement(1625..2665)
                     /locus_tag="P5549_05310"
     CDS             complement(1625..2665)
                     /locus_tag="P5549_05310"
                     /inference="COORDINATES: protein motif:HMM:NF040562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type B"
                     /protein_id="HRW12522.1"
                     /translation="MLQAGKSVTQKDDLLKKVQPEYLFHAVVNPKPEIAAAIRQLRLL
                     RTIDPKQYAFRKRQLPYVTCGIFNPPVRRTENFGWINHFILDLDHLESKNMLVSELKQ
                     KIVADTRVKLLFVSPGEDGVKIMFQLKQRCYDAALYSLFYRSFALSFAKQYQLEQVVD
                     NLTRDVTRACFVSFDPEAYYNPGPELVDLESYVDRSNPFDVRELKHSLREQEKEIPGV
                     EQDEVLVGPDVDQWAAIREKLKPSGKTKKEKIIFVPEEMEKAVEKVAERVKDLDIEIK
                     EVINIHYGKKFRFRYSVHEAEINLFYGKKGYTVVVSPRQGTQPELNEICAQVVNEIFN
                     GDCELKKDMVHD"
     gene            complement(2675..3658)
                     /gene="cas1"
                     /locus_tag="P5549_05315"
     CDS             complement(2675..3658)
                     /gene="cas1"
                     /locus_tag="P5549_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRW12523.1"
                     /translation="MDLIINSFGATLTKDNDTFVIIHEDGKQRVHPDGIKSISISKGA
                     QISSDAAIMAIEHEIDVLFVDNVGRPVGRLWSHKYGSVSTIRRGQLDFSLSKNAITWI
                     KGIIVSKLENQQAVLLTLRTKSPEEEAIVKKSIGRIEDYRDKIKSLQGEVVPDVAPSL
                     RGWEGAASRIYFDCINYFLPEKFQFSGRQQHPATDLFNCLLNYAYGVLYGKIEGELIK
                     AGIDPYVGVFHRDDYNRPVLVFDVIELYRVWADYVVIALAMQGAVNEDGYSVREDGSF
                     WLEGLGKRILIQSLNDYLDELVTIKGVSRSRNSHLGQYAQDLARLFKNHTT"
     gene            complement(3708..5159)
                     /locus_tag="P5549_05320"
     CDS             complement(3708..5159)
                     /locus_tag="P5549_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW12524.1"
                     /translation="MARFRRYKYKSVVHIKNRVMPINRFISYTLTSGQSEAIRQMESF
                     FRDDRQIYVLQGYAGTGKTTLLKGILDYLDAEKRPYKLMASTGRAARVLTNKTGRSAS
                     TIHSTIYEIDSARTDVQDDRKVLAFRLRQNQDAAETLYFIDEASMIADKTEMNQNLLF
                     DDGRFLDHILRYIGRRKIIFVGDSAQLPPVNCSFSAAMDADYLERTYHRKVTMVGLTE
                     VKRQEGFGGIITNATQLREKLNSGTLPPVSLKISGFDDIMPSVNIWKAVKDYSVEILN
                     QGFESEIFISYSNGGAHYLNSEIRRNIYKNPDTPLQADEWLMVIQNNYQTGYNNGQHL
                     RLLNWNNNGEKVGNISLVDAEVEDPQTGISKSVKLVYDLLFRKDPNLTLDEEKEFTKD
                     FAIRMRHMGIKPGTDSFLVRLISDKRLNALRVKFGYAITCHKAQGGEWDKVYMNIEPA
                     LEKMPRDLQYRWLYTAITRAASYLVVPNHPILY"
     gene            complement(5613..6662)
                     /locus_tag="P5549_05325"
     CDS             complement(5613..6662)
                     /locus_tag="P5549_05325"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HRW12525.1"
                     /translation="MKSVLVSVISAQTLPNFLLIKELRGLYSGMVFITTPDMEGKGNT
                     SMSRSRWIERATGIPEGSTPRVIVKEDSWPDIQQKLQEFFPDQSIHYTVNLTGGTKVM
                     TLAIFQHFAISGNMILYVPFPKNEYCELFPDIHTLPVKIKYRATLSEYLAAHGLYFTY
                     AGVFSKDQQTTELFFNEVQKSNFRLNEIDEIKHAHDMSIPEDRKYYSGGWFEEYVYHI
                     IKKGFNLSDRRIALGVNLYRNPEDIVSDNEYDLMFVFKNALYVIECKTSAGNSKEYAR
                     INLDRFLYKLGAITRDFGLRVNSYVFTLTKFRNDENRMSQQLEKRQSILGVRAIFDQV
                     SFYNTGEIIAKLKKG"
     gene            6779..12007
                     /locus_tag="P5549_05330"
     CDS             6779..12007
                     /locus_tag="P5549_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRW12526.1"
                     /translation="MNILEFHKKLIDNYKSYINSFLNVKDPQLLRFVEDELNNKKLWP
                     DPLVQFNPTFEKGRTIQNLVESGLLHRELQDIFYGYELYKHQEEAITLGASGKEFIVT
                     SGTGSGKSLTYIATIFNHILRSTESLDAKTQAVIVYPMNALINSQHEEFRKYERNYIK
                     RKLPTGTPFEEGEKSLIEQIRELKEITGDIFPIRYGQYTGQEDEATREKMRVNPPHIL
                     MTNYMMLELILTRGGRDMDIRNNILGNLRFLVFDELHTYRGRQGSDVALLIRRIKAGA
                     SHPVTCIGTSATMISSDTATLSEQKQEVAKIGSLIFGSDIHENQIVNEYLVKSLTTDS
                     PVTPASVALSVTRGIDPALPFTEFETNPVANWLEENIALERKEGTLVRRKPLTVEAIG
                     QQLQEYCGRAEAECNPYITKLLEWSNKINSDPSRKKNYLPYRIHQFIAQTGAVYATLG
                     NQNTRTAYLDAGLYSGNSDTFVFPLVFSRYSGHDFYCVTLDESQKRLFPRDFRNLEDP
                     EDEETTTKAGYIFISHQDDEEPVWDEARDLQDLPEAWFNPPRKDGSRSLKSDKRKSIP
                     AKIHFDSEGNFSFTEEKEFYGWFIAAPMAFDPTSGIIFDHGGEWAKLTKLGGEGRSTA
                     TTVLSFETISQLHAFGESFREQKLLSFTDNRQDASLQAGHFNDFVKVGQLRAAIWNAL
                     EKHGELDYTTIADRIFACMNIPQDQFAKNPALFPGPRKENEDAFKEFIMYRLLHDLRK
                     SWRVVLPNLEQCALLKIGYKFLEESVQDNTLWIQNELLAKMAPEERREFLAQVFDFFR
                     KSYALSFSMLEPAVINQNSKKIREKLIAPWTVDDSDKIDYPANIQVEKLDRSNLNLFG
                     GSAGYQSHFGRYLRNVASSHNIILRGKENYTPAVYALFDFLCDAGWLSRKYAKDENGE
                     QVYVYQLKVDYLLWQKGDGVSITPDLIKTRSYRPVTVKPNQYFQRFYKTDFHHLKPIE
                     GREHTGQIKNNKRQDREKLFRNGTISVLYCSPTMELGIDIADLNIVHLRNVPPSPANY
                     AQRSGRAGRSGQAALVMTYCSNYSAHDRHFFKTPEKMVAGAVSTPRMDLVNEELLMSH
                     LHALMLSRHSISKLNNSLGDLVDKEDLENLPVKEEIVQVFHLTEPQKQDIRNAFLKIL
                     EDTWFKNELTHRKPTWFSDTWIQRTIDRYFTDFDNALTRWRILYRNAILQFRAANEII
                     ENRVYAENHEIIEKAKNSRKQAERQMALLLNDMNDTNGRKSDNESEFYPFRYLAAEGF
                     LPGYNFTRLPIRTFLETRDGSGEFLSRPRVIALNEFGPRNVIYHDGAKFRVDRIILPE
                     AEAKLEMAKISPYTGYILMKEQYNYTVDPLVNRELTQGMDDHIHTDLVEITETVAFEQ
                     QRITCQEEERSRKGFDTRTYFYVEGGFDNTLEAIVKTGGEQLLHLHAFPAARLVHINF
                     KWRNSPEKGFALNLKSGHWQNKQQEAEEGNKDEVRRIKLFTTSTANVLYIQPVETLGL
                     QGGFAGVITLMYALKRAIENYFQVESNEIGVSVMGEGDSPNILLYEAAEGSLGVLSQL
                     VENPQVYKSVMHDAFEFCFMKEGVEIPEEELAPATYDDLLSYYNQSHHQVINRNYIRN
                     SLRLLKDSSVEVLSNRSFGSYGEQYHSLMASRDMNSSTEEAFLKYLYGKGLRLPDEAQ
                     PNIPKMYVLPDFLYKPNVCIFCDGTPHDEKQVMEGDKTKREALKNAGYQVLSWYYKEP
                     LDTFVARRPDIFKPVK"
     gene            12010..14862
                     /locus_tag="P5549_05335"
     CDS             12010..14862
                     /locus_tag="P5549_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRW12527.1"
                     /translation="MMHFDFKPGSLVSLRNRPWMVLPSGDPDLLLVKPLGGSDDEITG
                     IYLPLAHEADRPVSYNFVRPSASQPGEFTSARLLYNAARLSFRNVAGPFRCLGKLSFR
                     PRSYQLVPLIMALRQNPVRLLVADDVGIGKTIEALLIARELHERKEISRLAIICLPHL
                     CDQWQDELKSKFGIEAVIIRSGTASSLEKKIRTNENIFRAFPYQIISIDYIKTGTKLQ
                     VFLDHAPEFVIVDEAHTCARPAGANTSQMLRHNLVHRLVAQNPGQHLVMLTATPHSGK
                     QQEFQSLLGLLHPEFETTDIVEASPEERKRVARHFIQRRRGDVIKWLDEETAFPKRSS
                     TDVAYPVGNEYGILFNDILEYARSIVKENATDLRKQRFSFWEALALLRGVMSSPEAGV
                     SMLIKKAQKKRLSEEDDTDVESEEADSEVLDSGFSGADNLPVALLSKAKVTPDAESRR
                     LMDFAFRLGNVAGIAKDQKALMALKTAKQWIGDGIDPIIYCRYIHTARYLGEIFRSEL
                     SGRNYRNLHIEVITSELDDELRRAKIDAMKKSERRLLIATDCLSEGINLQDSFNAVIH
                     YDLPWNPNRLEQREGRVDRFGQQSPVIHVARIFGENNPVDGVVLDVLYKKSDEIRRST
                     GITVPLPEDSTTVMNAVMQAVLIRNAGIKQVARQLSLFENEEILETRKSLEEALNKAS
                     EKEKATRTIFAQHAIKATDIEGNLKEIDQAIGSVETVGNFVIGALQFLGVQADPKTHG
                     YLVYTTNIPERLRELLSDKQEIRVSFKSPTPEGYAYLGRNHPFTEQLAQTIINDALNG
                     MKGSAARSSVIRTREVESKTVLFQFRVRSVIAEQPADRRIVAEEMWLWGFRGEVGSGQ
                     FLTEEEASRLFMQAVASSNVENAEQEYWLQEELEWVNDETKFRAVTDPVAFGRAVHLV
                     DLHAGVRKLVGGSKYLPVEPVLPMDVMGLYILLPEL"
     gene            14867..19594
                     /locus_tag="P5549_05340"
     CDS             14867..19594
                     /locus_tag="P5549_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12528.1"
                     /translation="MNFTCINIQGNLISEDVLKKIEQGEASGQTASDFGLDSAANLRN
                     EMEYAWSRIKLDWKYFTGKSGNLPATDTYGTTTTRRWMTGFFSALGFDLVPRKSALTG
                     GNDQSYAISHVAGNIDDLPVHIVGFHDPSNPEKNTIDIRSSGGTSRLSPHATVQEYLN
                     VTEHLYGIAANGNLLRLMRDSGRLVKLTYLEFDLRRMLEEDKYSEFTLLFRLLHASRF
                     PRNQQETESCLLERYYNESIESGNRIRDGLSQAVQKSLLALGNGFLSDEANEKLREKL
                     RDGQLTPADYYRQLRRLVYRLLFLMVAEERDLVYDPAERNPEVLRKKRIYFDYYSISR
                     LRKLSRNRYLFEARYNDIWLGLLRTFTLFSEEARGRHLAIEPLGGDLFDQWAIEDLES
                     CLIGNHLLLECIRDLNEFTDVNGNLVSVNYRSLDVEEFGSVYEGLLDLHPVIDGLTLP
                     GLNMIRFTFHEGTDRKTTASYYTRPDLVNEIMKSAVIPVVEDKLKEAERIANSEWRVA
                     SGEWRVANSELRVANSEWKVVIQKYFKNLITNLSLEYVNLSRIAHLAKRDGFSGDDLP
                     IDKLLSQRRIVWDDFSTPSGRLLSSSQYRGGMGAQQQQGIQQLSANSQRKSEGDGNTF
                     AAVSPDRTMHPGADTTYDGLVFDRGQADTLLSKIHLSEKDQVLIWNRLPASIRYSLLA
                     HHHLLNIKVCDPAAGSGHFLLATARTLAWYHARVTSGEENPAPVVFRASLRQVIQHCI
                     YGVDLNPDAVELCKLALWIESHDSGKPLSFLDHKIRCGNSLVGVTDLTILQKGIPDEA
                     FDPVTGDDKTVARSLKRDNGRFRSFLSGRSDTAFQQQLDFGEAPVSHKYVTGMREVEQ
                     INQDSLDAVHRVRQRFEKMRSDREWFRDWEACNLWTAAFFYTYTPETRMAAPTSEHLH
                     KYLNHPTAVPGQVIGRADGLASKNRFFHWPLEFPEVFDLASGSSGFDVMVGNPPWERI
                     KLQQQEFFAIRDRNIANAPNAAARNRMIDELQITNPSLWREYQEALHAADSTGKFLRN
                     SGRNDLTAVGDINTYSVFAELFSSMISQRGKTGFIVPTGIATDDGNKAFFGAMVERNR
                     LVSLFDFENREAIFPGVHRSYKFCLLTLQGSDRGKEKASFGFFLTRVEHLQDKLRVFS
                     LSREDFLRLNPNTKTCPVFRTSVDAALTTDIYRRVPVLINETTGENPWGVSFMRMFDM
                     SNDSHLFRTRPQLEAEGFHLWGNKIVKKEPLPNGEWRLEKMWLPLYEGKMFMPFDHRY
                     ANVVITDNPLRAGQPEFAQYSDHINPNFTNEPRYWVDLNEVLRIKTDSYFLGFKNVTS
                     GTNERTFLATILPKSAIGHSTPIIFVNKETLLKSAFLGNCNSISFDYFARQKLGGINL
                     TYGYVQQFPVIPTIDSTDAILVEITPKLIELIYSSWDIKSFADDVWKEADEDLKAAIR
                     EQWEENKAATGGHEWDPPEWCEIDPEGCPLPPFKWDEERRAILKAELDAIYARLYGLN
                     RKQLRYILDPADLTPRELENILDPWEEVTNPLDPEGYAHRSAQSTFPGETFRVLKDKD
                     IRQFGEYRTRRLVLEAWERQNKS"
     gene            19719..20207
                     /locus_tag="P5549_05345"
     CDS             19719..20207
                     /locus_tag="P5549_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12529.1"
                     /translation="MNYKLVKIVELSGDKASVYSLYTEDEKCTLFEKFMRENSKSFKS
                     ETIDILKRLNSIASKEGAREQYFKTREGKPGDLVCALYDRPNSHLRLYCIRLGGSLII
                     LGGGGHKPKTIRALQSDPKLSSENSLMVKLSGELLHRVKSKEILYVNDYMDLEGDLDF
                     EF"
     gene            20228..20686
                     /locus_tag="P5549_05350"
     CDS             20228..20686
                     /locus_tag="P5549_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW12530.1"
                     /translation="MKKAESYSSNLLREFLGDISLKEQEKTDKRMRLAAVIDNGIKAK
                     GWKKSDLAKALNKRPSEISKWLSGTHNFNSDTLFDIEEALNIRLINLFEEPKVQVTRY
                     NLFVSSRVPTSAGVLENSSPLGNLLIAQDPTQIYSSRSTIDQRTQTDFRA"
     gene            20692..21114
                     /locus_tag="P5549_05355"
     CDS             20692..21114
                     /locus_tag="P5549_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12531.1"
                     /translation="MTNATKVTFQIKGIDLVDIQMNQLKQPLPAQTTFHFNINLEQLI
                     NPENKLVIVVATVVIIHEDKTTILASIKASCVFDVVNFDEFVAEDTRKVTFPESAVIT
                     FNSITISTVRGLMFSQFKGTHLHTAILPVVDPSSFIKN"
     gene            21400..22908
                     /locus_tag="P5549_05360"
     CDS             21400..22908
                     /locus_tag="P5549_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRW12532.1"
                     /translation="MAIILQRQISEDEKKQIIERFGRKCYATGHEIPITEEIHYDHIM
                     AFSDDGASEIDNIAPMCQMHNLQKGRLPLQDFRIKLRLDDFFKHGQSHTLKHELQHFK
                     DQGIINNFGENVYSIITDNHIELEFLNKKETFRLYSCPITKWKYFYATLPVDVINSDD
                     DEDGKIGLQPRYLIQDKVFDLYRHFQRNTVLNPSIGRLHNNKILIFDGQHKIASLLWG
                     ERKYFELKIYINPDPKSLNQTNISAHDKFAQTRFFASIMVAKLGSQFGKQFDEYKNLE
                     DGVKKTEAGFVKFVKNSEQLSNADINKRFTSFIYDSILDESTNKIVRLVSKSNRSTME
                     YPITMDMLSKSIFSNFLYRNPTEDDLTSDSYKRDKEISNAIRLCNILDEEILHKWDAT
                     KTENDLVQNKLNRMFRSKSIMAWAELLRDAIAAKLEITDQDEKAQIFYRDITDESFDK
                     VKKVVRRLVSWSVWDSPVNSEIDRIFADNKSEVKKYFKDKGLTTGYLLGATE"
BASE COUNT         6500 a         5769 c         5225 g         5737 t
ORIGIN      
        1 taccggcgac atcaagggtg tcttaatcct tctcttgctg gaacaacctc tccgacacaa
       61 agaaaaaaaa tcaaggaaag aagactacca agtcttaatc cttctcttgc tggaacaacc
      121 tctccgactg tggacttcat atactcctca aaaacaaaca aataacgcct gtatttaaag
      181 aattgttaca atttttaaca aaaaacaccc tcattttttg agcctgcaaa attactacaa
      241 tatatttaaa ttttagttcc attaaaccaa catgtcaaag aactttttta tcactcattt
      301 ggataggtat ccccaaattt aatcattcat acagatcacc tttcactcat tatcgtcccc
      361 cgctttgaga gtactcctga gtgttttttt ttaaggatat cactccaaaa ccaacatctg
      421 aaccgccacc gattgaaaaa aacccgggga aaaccacatt agacacaaat tcaatatcat
      481 aaaaagtcaa tgaatccgat tcatcccccc cggtcctatt tccctgatat atatcaacaa
      541 ctatggtgtc cacaccttcg ctggtgtccg cacccaagat ttccaaacca tgaagaacat
      601 ccctacgaag aaacttgtca gcttcctttt gtaatgaatc agagtgacca aaactaccgc
      661 ggtcctttga cccgaaaacc cagtctctga tgttgtattg gtaataggaa tctgttacac
      721 caatttcagc cgaacggaat ttaacctcag acaccttaaa tagttccttc cttggtctaa
      781 tccacacata ccaatccgta tcggtaaaca aatttctcac aggaaaagga atcattccat
      841 aacaaaacag gcaggtctta gcctctacat ttttatactg acaaacagga tgtacataat
      901 tccctacctc agtctccttc cagcgtttaa taatccctga caatcccaga ttattctcaa
      961 tgacctcatt caatacagga aattcttccc tggtaaggga aacattgaat tccaccgaca
     1021 atatcgaaat ctttttaagt tccatcatat gaaaagggta tttttattct tcaaggcaag
     1081 atcaaaatca atattttgcc cgataacctt catcgccctt atctcatcgg ttgaaactgg
     1141 taccagaata atgctgtcag aattatcgta acattcctgc acttctttca aatccttcct
     1201 gatctcatca aaaaccggtc tttctgtact ggcaatgaaa atcgatttct gcaccctggt
     1261 acaccccttc ctgatcaggt acttggcaac aagagttctc accttgttgc tttcaatatc
     1321 gtacatgatg aaatatatca tttgtcttgg attattattc tgtgtattca gcacccccaa
     1381 aaccttgtcg atacgttcag gaagcgacgg aagttcatcg accccttctc cctgacgcaa
     1441 accacgcaat cctgaaattc cggccttccg aagcttcatc atcctctgct gaaacgttat
     1501 ttcatcgcga ggctttctta tttttgtgct tttaattacc atatcatttg tccattaacg
     1561 tgcccgccaa cctgtacaat gaaatcatat gcaaaataga ctacattcat tctaactggc
     1621 cttttcaatc atgtaccatg tcttttttca gctcgcaatc tccgttaaat atctcattca
     1681 ccacctgcgc acagatctca ttcagttcag gctgtgtccc ctgccgcgga gaaacaacca
     1741 cggtatatcc cttcttcccg taaaaaaggt tgatttcagc ctcgtgtacg ctgtacctga
     1801 acctgaattt cttgccatag tggatattga tgacctcctt gatctcaata tccaggtctt
     1861 taaccctttc agccactttc tcaaccgcct tttccatttc ctccggaacg aaaatgattt
     1921 tttccttctt cgtttttccg gaaggtttca gcttttccct gatggccgcc cactgatcca
     1981 catcgggacc aaccagcacc tcatcctgct ctaccccggg aatttccttt tcctgctccc
     2041 tcagggaatg tttcagctct ctgacatcga agggattact cctgtcaaca tacgactcaa
     2101 ggtcaaccag ttccggcccg ggattgtaat acgcttcggg atcaaaacta acaaaacagg
     2161 cacgcgttac atcacgcgtc agattatcga ccacctgctc aagctggtat tgctttgcga
     2221 aggacagtgc aaacgatctg taaaacaagg aatacaaagc cgcatcataa catctttgtt
     2281 tcaattgaaa cataatctta accccatctt caccgggaga aacgaaaagc agctttaccc
     2341 ttgtatcggc cactattttc tgtttcaatt cactcactaa catatttttg gactccaaat
     2401 gatccaaatc cagaataaaa tggttgatcc aaccgaagtt ctctgttctt ctgaccggag
     2461 gattgaatat tccgcaggtc acataaggaa gctgtctttt ccggaatgca tattgtttag
     2521 gatcgatggt cctcaataac cggagttgcc tgatggctgc ggctatttcc ggtttgggat
     2581 tcaccactgc atgaaaaagg tattcgggtt gcaccttttt caacagatca tctttctgtg
     2641 taacagattt tccagcctga agcatatcct tcttttatgt tgtatggttc ttgaaaaggc
     2701 gtgccaggtc ctgcgcatac tggcccagat ggctgttccg gctgcgcgaa acccctttga
     2761 tggtgaccag ctcatccaaa taatcgttca gcgactgtat gaggattctc ttacccaatc
     2821 cttccagcca gaaggaacca tcctccctca cgctgtaacc atcctcattg acggctccct
     2881 gcatggccag ggcgatgacc acgtaatcgg cccacaccct gtaaagctca attacgtcaa
     2941 aaaccagcac gggccggtta taatcatcgc ggtggaagac tcccacgtaa ggatctattc
     3001 cggccttgat aagttcccct tcaatcttgc cgtacaaaac gccataggca taattcagaa
     3061 ggcaattgaa aaggtcagtg gccggatgtt gctgccttcc cgaaaactga aacttctcgg
     3121 gaagaaagta attgatgcaa tcgaaataga tgcgggaagc ggctccttcc catccccgca
     3181 gggaaggggc gacatcagga accacctcgc cttgcagcga cttgatctta tcacggtaat
     3241 cctcaatcct gccgatagac ttcttaacga tcgcttcttc ttccggagat ttggtcctta
     3301 gagtcaggag aaccgcttgc tgattttcca gcttactgac gatgattccc ttgatccacg
     3361 tgatggcatt tttggacagg ctgaagtcga gttgtccccg cctgatggtg gataccgatc
     3421 cgtatttgtg actccagaga cggccgaccg gacggccgac attgtccaca aaaagaacgt
     3481 caatctcatg ttctatggcc atgatggcgg cgtcacttga gatctgtgcc cccttactga
     3541 ttgatatgct tttaatcccg tcagggtgaa cgcgctgctt cccgtcttca tggataatga
     3601 caaacgtgtc gttgtccttg gtcagggtag cgccgaaaga attgatgatt agatccattg
     3661 catagctttt aaaattactg ccaattattc aaaccataac catacgttta gtacaatatc
     3721 gggtgatttg gaacaacaag gtaactggcc gcacgggtaa ttgcggtgta aagccaccgg
     3781 tattgcaaat cgcggggcat cttttccaat gctggctcaa tattcatata aactttgtcc
     3841 cattccccac cctgagcctt gtggcaagtg atggcatacc cgaacttcac gcgcaaagca
     3901 ttaagccgct tgtcactgat cagtcgaacc agaaaactgt cggttccggg tttaattccc
     3961 atatgacgca tcctgatggc aaaatctttt gtgaactctt tttcttcgtc aagggttagg
     4021 ttcggatcct tacggaaaag taaatcatac accagtttaa cactcttgct tattcctgtt
     4081 tggggatctt caacctcagc atccaccagt gatatatttc ctactttctc cccgttgttg
     4141 ttccagttca gtaacctcag gtgctgtccg ttgttgtagc cggtctggta attgttctgg
     4201 atgaccatca gccattcatc agcctgaaga ggagtatcgg gatttttgta tatattccgg
     4261 cggatctctg aattcaggta gtgggcccca ccattcgaat aactgataaa gatctcactt
     4321 tcaaatccct gattgagaat ctccacggaa taatccttca cagccttcca aatgttcact
     4381 gacggcatga tgtcgtcgaa acctgaaatc ttcagggaaa cgggaggcag tgttccggaa
     4441 ttcaattttt cacgcaactg tgttgcgttg gtgataattc ctccgaaccc ctcctgccgc
     4501 ttcacctcgg tgagccccac catggtcacc ttccggtgat aggtcctttc cagataatcc
     4561 gcatccattg ccgctgaaaa ggaacagttt accggaggca gctgggcgct gtcaccaacg
     4621 aatatgattt tccttctgcc tatatacctg aggatatggt ccaggaatcg tccgtcatca
     4681 aaaagaagat tctggttcat ctccgttttg tcagcaatca tggaagcctc atcaatgaaa
     4741 tacagggttt ctgctgcatc ctgattttgc cgaagtctga aagccaagac tttacggtca
     4801 tcctgaacat cggtacgtgc actgtcaatt tcataaatgg tgctgtgaat ggttgaagcc
     4861 gacctcccgg ttttgttggt caggaccctc gcggctcgtc cggtcgaggc cattaacttg
     4921 tagggtcttt tttcagcatc aaggtaatcc aggatacctt tcagcaaagt tgtttttccc
     4981 gtaccagcat atccctgtaa cacataaatc tggcggtcat cgcgaaagaa ggactccatt
     5041 tgcctgatgg cctcgctttg gccgctggtc agggtgtagc tgataaacct gttgataggc
     5101 atgactctgt ttttaatatg caccacactt ttatacttat accttctgaa cctggccact
     5161 gtccgcatcc cccaaccggg aatactccct gaacctgcaa aatctgaatt cagatacttt
     5221 cgtggagcca ttttcaacgg ataacctttc agaagtagag gcaatgcatg aaattcctct
     5281 cttatggagt ctttttacaa gtgcgtagca caatgttaat tcacccataa ggtgaactgc
     5341 acaatgtttg ggattttggc tctttataag ttccaaaacc ttttccacat atatatcagc
     5401 aacttgctcg atttcttcag ctgaccacca gggatccaca ttgggaaaga aaagatccat
     5461 ggtctcacca taatcagcgg cagccctttc ttggtccttg ctccaccttg aagacggatg
     5521 gtttgaaaga tttatcagca tcttacatat ttaatgaatt gttttctgta ctttttgtca
     5581 tcacaaaatc aggtgcaaat ctttttcatg ctttatccct ttttgagttt tgcaataatt
     5641 tcaccagtat tataaaatga aacctggtca aaaatagctc tgacccctaa aatactctgc
     5701 cgtttttcaa gctgctggct catcctgttt tcatcattcc tgaattttgt aagcgtgaaa
     5761 acatacgaat ttactctcag cccaaaatca cgggtaatag cccccaactt ataaagaaat
     5821 ctgtccaggt tgattcgggc atattcttta ctgtttcctg cagatgtttt acattcaatg
     5881 acataaagtg catttttgaa tacaaacatc aaatcatatt cgttatccga aactatatct
     5941 tcgggattac gataaaggtt cacccccagg gcaattcttc ggtctgataa attaaagccc
     6001 tttttgataa tatggtacac gtattcttcg aaccaccctc cggaataata tttcctgtct
     6061 tctgggatac tcatatcatg agcatgttta atttcgtcaa tttcattaag tctgaaattg
     6121 gatttctgca cttcattaaa aaaaagctcc gttgtttgtt ggtctttcga aaacacaccg
     6181 gcatatgtaa aatagaggcc atgcgctgcg aggtattctg acagcgtagc cctgtacttt
     6241 attttgaccg gaagagtgtg aatgtctgga aataactcac aatattcatt cttcggaaaa
     6301 ggaacataaa gaatcatatt cccgctgatt gcaaaatgct ggaaaatcgc cagggtcata
     6361 acctttgtcc ccccggtgag atttaccgta taatgaatgc tttgatccgg gaaaaactcc
     6421 tgtagttttt gctgaatatc aggccaggaa tcttccttaa caatgacacg gggcgttgat
     6481 ccttccggaa tcccggtagc cctttcgatc catctgctcc tgctcatcga agtattcccc
     6541 ttgccctcca tatccggggt ggtaataaaa accattccgg aatacaaccc tcttagttcc
     6601 ttaatcaaca aaaaattggg caatgtttga gcggaaataa cactgactaa aacagacttc
     6661 atcccaggga attaattgta gtcgtaaata taggaaaatt taacaatctt ttcccatctt
     6721 taaggttccg gaaaccaagc cgaagtatta tcgacatata taaacatgcc ctgacctgat
     6781 gaacatactc gaattccata agaagttgat cgacaattat aaaagctaca tcaacagctt
     6841 tttgaatgtc aaggatccgc agcttctccg gtttgtggag gatgaattga acaataaaaa
     6901 actgtggccc gacccgctgg tgcagttcaa ccccactttc gaaaaaggac ggaccatcca
     6961 aaatctggtg gagagcggtc tgctgcaccg tgagcttcag gatatctttt atggttacga
     7021 gctatacaaa caccaggagg aggccatcac cctcggtgca tcagggaagg agttcattgt
     7081 cacctccggg accggctccg ggaaatcgct gacctacatt gccaccatct ttaatcatat
     7141 cctcaggtca accgaatctc ttgatgcgaa aacacaggcg gtcatcgttt accccatgaa
     7201 cgccctgatc aactcgcaac acgaagaatt ccgcaaatac gagcgcaact acatcaaaag
     7261 gaaattgccc acgggaactc ccttcgaaga gggggagaaa agcctgatcg agcagatcag
     7321 ggaattgaaa gagatcacgg gggatatctt ccccatccgc tatggccagt acaccggaca
     7381 ggaggatgag gcgacgaggg agaaaatgcg ggttaatcct ccccatattc tgatgaccaa
     7441 ctacatgatg ctggagttga tcctgacccg tggaggcagg gacatggaca tcaggaacaa
     7501 catccttgga aacctgcgtt tcctggtctt cgatgaactg cacacctacc ggggccgcca
     7561 gggatcggac gtcgccctgc tgatcagaag gatcaaggcg ggggcaagcc acccggtgac
     7621 ctgcatcgga acatcagcca ccatgatttc gtccgataca gccacccttt ccgaacaaaa
     7681 acaggaggtg gcgaaaatcg gaagcctcat cttcggaagt gatatccacg agaaccagat
     7741 cgtcaacgaa tacctggtca aaagcctcac tacagattca ccggtaaccc ccgcaagcgt
     7801 ggccctgtcc gtgacccggg gaattgatcc ggcgttaccc ttcacggaat ttgaaaccaa
     7861 tccggtggcc aactggctgg aggagaatat cgccctcgaa cgcaaagaag gaaccctggt
     7921 ccggcgaaaa cctctcaccg ttgaagccat cggccaacaa ttgcaggagt attgcggaag
     7981 agcagaagcc gaatgtaatc cctatattac aaaactgctg gaatggtcca acaaaatcaa
     8041 ttccgaccct tcccggaaaa agaattacct cccctaccgg atccatcaat ttatcgccca
     8101 gaccggagcc gtctatgcca ccctggggaa ccagaacacc cggacggcct acctggacgc
     8161 gggtttgtat tcgggcaatt ccgacacctt cgtatttccg ttggtattca gccgctactc
     8221 gggtcatgat ttttactgcg tcaccctcga tgagagtcag aaaaggcttt tcccgcggga
     8281 tttccggaat ctcgaagatc ctgaagacga ggaaacaacg acaaaagcag gctacatttt
     8341 catttcacat caggatgatg aggaaccggt ttgggatgaa gcacgcgatc tgcaggacct
     8401 tcccgaagcg tggtttaacc cgcccagaaa agatggatcc cgttcgctga agtccgataa
     8461 aagaaaaagc atcccagcaa aaattcattt tgacagcgaa ggcaactttt catttacaga
     8521 ggaaaaggaa ttttatggtt ggtttatcgc cgctcccatg gccttcgatc ccacttcagg
     8581 aatcatcttt gaccatggcg gcgaatgggc caaactgacc aagctgggag gagaagggcg
     8641 cagtacagcc actacggtcc tttcatttga aaccatatcg cagctccatg cctttggcga
     8701 atcattcagg gagcagaagc tgctgagctt taccgataac cggcaggatg cctccctgca
     8761 ggcaggccac tttaacgatt ttgtaaaagt cggacagcta agggccgcca tctggaacgc
     8821 cctcgaaaaa cacggagaac tcgactatac cacgattgcc gaccggattt tcgcatgtat
     8881 gaacatcccc caggatcagt ttgccaaaaa ccctgctctg tttccgggtc cacggaagga
     8941 aaatgaagac gccttcaagg aattcatcat gtaccgtctg ctccacgacc tgaggaaaag
     9001 ctggcgcgtg gtattgccca acctggaaca gtgcgccctg ttgaaaatag ggtacaaatt
     9061 tctggaagaa tcggttcagg acaataccct ctggatacaa aatgagttgc tggccaaaat
     9121 ggccccggaa gagcgccgcg agtttctggc acaggtgttt gatttcttcc ggaaatcata
     9181 cgccctgagc ttctccatgc tcgaaccggc cgtcatcaac cagaactcca agaaaatcag
     9241 ggaaaaactg atcgctccct ggacagttga cgactctgat aaaattgatt atcctgccaa
     9301 tatccaggtc gagaagctgg accgttccaa cctgaacctt ttcgggggaa gcgccggtta
     9361 ccagagccat ttcggacgat acctgagaaa tgtggcctcc agccataaca tcatcctccg
     9421 gggaaaagaa aactataccc cggccgtcta tgctttgttt gattttctgt gtgacgccgg
     9481 atggttatcg cgcaaatacg ccaaagacga aaacggggag caggtttacg tgtatcagtt
     9541 gaaggtcgat tacctcctct ggcagaaggg ggacggagtg tccatcacgc ccgacctcat
     9601 caaaaccaga tcctacagac cggtcacggt taaaccaaac cagtatttcc agcggtttta
     9661 caagaccgat ttccaccacc tgaagcctat tgaaggacgg gaacacaccg gccagatcaa
     9721 aaacaacaaa agacaggaca gggagaaact cttccggaac gggaccatca gtgtgctgta
     9781 ttgctcccct accatggagc tggggatcga catcgccgac ctgaacattg ttcacctcag
     9841 gaatgttccg ccatcaccag ccaactacgc ccagcgaagc ggacgtgccg gccggagcgg
     9901 acaagccgct ctggtgatga cctactgctc caactacagt gcccacgacc gccacttttt
     9961 caagacaccc gaaaagatgg tggctggagc tgtctccaca ccccgcatgg acctggtcaa
    10021 cgaggagttg ctgatgagcc acctccacgc gttgatgctt tcacggcatt ccatttccaa
    10081 actgaacaat tccctgggcg acctcgtcga caaggaagat ctggaaaacc ttccggtcaa
    10141 agaggaaatt gtgcaggttt tccatcttac tgagccccag aaacaggaca tcaggaacgc
    10201 tttcctgaaa attctggaag acacctggtt caaaaatgag ctgacccacc ggaaacccac
    10261 ctggttcagc gacacctgga ttcaacgcac catcgacaga tatttcaccg actttgacaa
    10321 tgcccttaca cgctggagaa ttctctaccg caatgccatc ctgcagttca gggcagccaa
    10381 tgaaatcatc gaaaaccggg tgtacgccga aaaccacgaa atcatcgaaa aggccaaaaa
    10441 ctcacggaag caggccgaac ggcagatggc tctgctgttg aatgatatga acgacaccaa
    10501 tggcaggaag agtgacaacg aatcggaatt ctatcctttc cgctacctgg cggccgaagg
    10561 attcctgccc ggttataact tcacccggct tcccatccgc accttcctgg aaaccaggga
    10621 tggttccgga gaattcctgt ctcgtcccag ggtcattgcc ctgaatgagt ttggccccag
    10681 aaacgtgatc taccacgacg gcgcaaaatt cagggtggac cggattatcc ttccggaagc
    10741 ggaagcgaaa ctggaaatgg ccaaaatcag cccttacacg gggtatatcc tgatgaagga
    10801 gcagtacaac tacacggtcg atccgctggt caacagggaa ctgacccagg gcatggacga
    10861 ccacatccat accgatctgg tggaaattac cgaaacggta gcctttgaac agcagcggat
    10921 cacctgtcag gaagaggaga gaagccggaa gggattcgac acccgcacct atttctatgt
    10981 ggaagggggg tttgacaaca cgctggaagc catcgtcaag accggagggg aacaactgtt
    11041 gcacctgcat gcctttccgg cggcaaggct ggtccacatc aatttcaaat ggaggaattc
    11101 cccggagaag ggattcgccc tgaacctgaa gagcggacac tggcagaaca aacaacagga
    11161 agccgaagaa gggaataagg atgaggtcag gcggatcaag ttgtttacga cctccacggc
    11221 caacgtcctc tacattcagc cggtggagac cctgggtctg cagggcggtt tcgcaggagt
    11281 aatcaccctg atgtatgccc tgaaacgggc catcgaaaac tacttccagg tggaatcgaa
    11341 cgaaataggg gtttccgtca tgggcgaagg cgattctccc aacatcctgc tttacgaagc
    11401 ggccgaagga agcctgggcg tcctctccca gctggttgaa aatccgcaag tctacaagtc
    11461 ggtcatgcac gacgctttcg aattctgctt catgaaagag ggtgtggaaa taccggaaga
    11521 agagcttgcc ccggccacct acgacgattt gctgagctac tacaaccaaa gccaccacca
    11581 ggtcatcaac agaaattata tccggaactc tttaaggctg ctgaaggatt cttccgtgga
    11641 agtgctctcc aaccgttctt tcggcagtta tggcgaacaa taccattcct tgatggcatc
    11701 aagggatatg aatagcagca cggaagaggc attcctgaag tatctctacg gaaagggcct
    11761 ccggctgccc gatgaagccc agccgaatat ccccaaaatg tacgtcttgc ccgacttcct
    11821 ttataagccg aatgtctgta ttttctgtga cggcactccc catgacgaaa aacaggtcat
    11881 ggagggtgac aagactaagc gggaggcact gaaaaatgcc ggataccagg ttctttcgtg
    11941 gtattacaaa gagcccctag atacgtttgt agccaggcgg cccgatattt ttaaacctgt
    12001 caaatgagca tgatgcattt cgatttcaaa cccggtagcc tggtcagcct gcgaaaccga
    12061 ccctggatgg tgttgccctc cggggatcct gacctgttgc tggtcaaacc cctgggagga
    12121 tccgacgacg agatcacggg gatttacctc cccctggccc acgaagccga ccgcccggtt
    12181 tcctacaatt ttgttaggcc gtcggcaagc cagcccgggg aattcacttc ggcacggttg
    12241 ctctataatg ccgcacgcct gtcgttcaga aatgtggcag gccccttccg ctgcctgggg
    12301 aaattatcct tcaggccccg ctcctaccag ctggtccccc tcatcatggc actcaggcag
    12361 aatcccgtcc gtctgctggt ggccgacgat gtgggcatcg gcaaaaccat agaggccttg
    12421 ctgatcgccc gggaactcca cgaaagaaaa gagatcagcc ggctggccat catctgtctc
    12481 ccccaccttt gcgaccagtg gcaggatgag ctgaaatcga aattcgggat cgaagcggtc
    12541 atcatccgtt cgggaaccgc cagttccctg gagaagaaga taaggacgaa tgagaatatt
    12601 ttcagggcat tcccctacca gatcatctcg atcgactaca tcaagaccgg caccaagcta
    12661 caggtgttcc tcgaccatgc cccggaattt gtcattgtcg atgaggccca tacctgtgcc
    12721 cggcctgcgg gagccaacac ctcccaaatg ttgaggcaca acctggtgca ccggctggtt
    12781 gcccaaaatc ccggacagca cctggtcatg ctgaccgcca cgccccacag cgggaaacag
    12841 caggagttcc agtccctgct cgggctcctt catccggaat ttgaaaccac cgatatcgtg
    12901 gaggcctctc cggaagaaag aaaacgggta gcccgtcatt tcatacagcg caggcgcggc
    12961 gatgtcatca aatggctcga tgaagagacc gccttcccca aaagaagttc aacagatgtt
    13021 gcttatccgg tagggaatga atacgggatc ctttttaacg acatcctgga atatgcccgt
    13081 tccatcgtga aagaaaacgc cactgacctc cggaagcaaa ggttcagttt ctgggaagcc
    13141 ctggcccttc tcagaggcgt catgagcagt cccgaggccg gggtgtccat gctgatcaag
    13201 aaagcgcaga aaaagcggtt gtcagaggaa gatgataccg atgtggagag tgaagaggcc
    13261 gacagcgaag tgctcgattc aggcttttcg ggcgccgata acctccctgt ggccctcttg
    13321 tcgaaagcca aagttacccc tgatgccgaa tcccgcaggc tcatggattt tgctttccgg
    13381 ctgggcaatg tcgctggcat cgcaaaggac cagaaagccc ttatggcgct gaaaacggcc
    13441 aaacagtgga tcggtgatgg aattgacccg atcatctatt gccgctatat ccataccgca
    13501 agatacctcg gggagatttt tcgcagtgaa ttgtcgggac ggaattaccg gaacctgcat
    13561 attgaggtca tcaccagcga actggatgat gagctgcgcc gggcaaaaat tgatgcgatg
    13621 aaaaagtcgg aacgtcgcct gctcatcgcc accgactgct taagtgaggg catcaacctt
    13681 caggactcct tcaatgcagt catccattac gacctgccct ggaatcccaa tcgcctggaa
    13741 cagcgcgaag gccgggtcga ccgttttggc cagcaatcgc cggtcatcca tgtggccaga
    13801 atcttcgggg aaaacaatcc cgtcgacggg gtggtcctcg atgtcctcta taaaaagtcg
    13861 gatgagatcc gccgatccac gggaatcacc gtacccctgc ccgaagacag caccacggtc
    13921 atgaatgccg tgatgcaggc cgtcctgatc agaaatgccg gaattaaaca ggtggcccgc
    13981 cagttgtcac tgtttgaaaa cgaggagatc ctggaaaccc ggaaatccct ggaagaggcc
    14041 ctgaacaagg cttcggaaaa ggaaaaggcc acacgcacca tcttcgccca gcatgccatc
    14101 aaggccaccg atatcgaagg gaatctgaaa gagatagacc aggctatcgg aagtgtggaa
    14161 accgtcggga actttgtcat cggggcgttg cagtttctcg gcgttcaggc cgatcccaaa
    14221 acgcacggat acctggttta cacgaccaac attccggagc gactaaggga actgctttcc
    14281 gataaacagg aaatacgggt ttccttcaaa tccccgactc ccgaagggta tgcctatctg
    14341 ggccgcaacc atccgtttac cgaacaactg gcgcagacca tcatcaacga tgccctcaac
    14401 ggcatgaagg ggagtgccgc acgttcctcg gtgataagga cacgggaagt agagagcaaa
    14461 acggtccttt tccagttcag ggtgcgaagt gtcatcgccg aacaacctgc cgaccgccgg
    14521 attgtggccg aggagatgtg gctgtgggga ttccggggag aagtcggttc agggcaattc
    14581 ctcacggaag aagaggcctc ccgcctcttt atgcaggcag ttgcctcgtc gaatgtggaa
    14641 aatgccgaac aggaatactg gctgcaggag gagctggagt gggtgaatga cgaaacaaaa
    14701 ttcagggcgg ttaccgatcc tgtggcgttc ggtcgggccg tccatctggt cgacctccat
    14761 gccggcgtgc gtaagctggt aggcggcagc aaatacctgc ccgtggagcc tgtgcttccc
    14821 atggatgtga tgggattata cattttatta cctgagctgt aacgccatga actttacctg
    14881 catcaacatc cagggcaacc tgatatcgga agatgtcctc aaaaaaatag aacagggaga
    14941 agcttcagga cagaccgcct ccgactttgg cctcgattcg gccgccaacc tgcgcaacga
    15001 aatggagtat gcctggagcc gcatcaaact cgactggaaa tacttcaccg ggaaaagcgg
    15061 caacctgcct gccaccgata cctatgggac gaccacgacc cggagatgga tgaccggctt
    15121 tttcagtgcc ctcggatttg acctggttcc ccggaaatcg gcccttaccg gcggaaacga
    15181 ccagagttat gccatcagcc atgtggcagg caacatcgac gacctgccgg tccacatcgt
    15241 gggcttccat gatccttcaa atcccgaaaa aaacaccatc gacatcaggt caagcggggg
    15301 gacctcccgc ttgtcgcccc acgcgaccgt tcaggaatac ctgaatgtga cggaacatct
    15361 ttacggaatc gcggccaacg gcaatcttct caggctgatg cgcgacagcg gccgcctggt
    15421 caaactcacc tatctggaat ttgacctcag gcggatgctc gaagaagaca aatacagcga
    15481 gttcaccctt ctcttcaggc tgctccatgc ctcccggttt ccccggaatc agcaggaaac
    15541 ggaatcctgc cttctggaaa ggtattacaa cgaaagcatt gaatcgggga accggatacg
    15601 tgacggactg agccaggccg ttcagaaatc gctgcttgcc ctcggaaacg gctttctcag
    15661 tgacgaggcc aatgaaaagc tccgcgaaaa gctgcgtgac ggacagctta ccccggccga
    15721 ctattaccgg caactccgcc ggctggtcta ccgcctgctc ttcctgatgg tggccgagga
    15781 aagagatctg gtatatgatc ctgcagaaag gaatccggaa gtcctcagga agaaaaggat
    15841 ttatttcgat tactacagca tctccaggct ccgcaagctc agccggaacc ggtatctctt
    15901 cgaagcccgt tataacgaca tctggctggg actgctgcgt accttcacgc ttttcagtga
    15961 ggaagcacgt gggcgccatc tggccattga gcccctggga ggggatcttt tcgaccagtg
    16021 ggccattgaa gatctcgaaa gttgcctgat cggcaaccat cttctgctgg aatgcatcag
    16081 ggacttgaac gagtttaccg atgtgaacgg gaacctggtc tctgtcaact accgttccct
    16141 cgatgtcgag gagtttggtt cggtgtatga agggttgctc gatctgcatc cggtcattga
    16201 tggcttgacc cttcccggtc tgaatatgat ccgctttact ttccatgaag gtaccgaccg
    16261 gaaaaccacg gcctcctatt acacccgtcc cgacctggtc aatgagatca tgaagtcggc
    16321 ggtgattccg gtggtggaag acaagcttaa agaagcagag cgaatagcta atagcgaatg
    16381 gcgagtggcg agtggcgagt ggcgagtagc taatagcgaa ttgcgagtgg ccaatagcga
    16441 atggaaagtg gtaatacaga aatattttaa gaatttaatc actaatctaa gcctggaata
    16501 tgttaaccta tcaagaattg cacatctggc aaaaagggat ggatttagcg gagatgattt
    16561 accgattgac aaactccttt cccaaagaag aattgtatgg gatgacttct caactccgtc
    16621 gggccgcttg ctcagttcca gccaatatcg cggagggatg ggggcgcaac aacaacaagg
    16681 aattcaacaa ctttctgcga atagccaacg gaagtctgaa ggagacggaa acacatttgc
    16741 tgctgtcagc ccagatcgga ctatgcaccc aggagcagat acaacctatg atggacttgt
    16801 ctttgaccgc gggcaagcag atactctcct ttcaaagatc catctctcag aaaaagacca
    16861 agtcctgatt tggaaccgat tacctgcatc tattcgctat tcgctactcg ctcatcacca
    16921 tttgttgaat ataaaagtct gcgatcccgc tgccggttcc ggccatttcc tgctggccac
    16981 ggcacgcacg ctggcctggt accatgcccg ggtgaccagc ggagaggaga atcccgcccc
    17041 cgtggtcttc cgtgcttcgc tcaggcaggt catccagcac tgtatttacg gtgttgacct
    17101 caatcccgac gctgtggaac tctgcaaact ggccctctgg atcgaaagcc atgacagcgg
    17161 gaaacccctg agcttcctgg accataaaat ccgttgcggg aacagcctgg tgggcgtgac
    17221 cgacctgacc atcctgcaga aaggaattcc cgacgaagct tttgatccgg tgaccggcga
    17281 tgacaagacg gtggcccgat ccctcaaacg ggacaatggc cgcttccggt cgtttttatc
    17341 cggaaggagc gataccgcct tccagcagca acttgatttc ggggaagccc cggtttccca
    17401 taagtatgtg acgggtatgc gcgaggtgga acaaatcaat caggactcgc ttgatgcggt
    17461 tcacagggtc agacaacggt ttgaaaaaat gcggagcgac cgggagtggt ttcgcgactg
    17521 ggaggcctgc aacctctgga cggccgcctt cttctatacc tacacccccg aaacccggat
    17581 ggctgccccg accagcgaac atctgcacaa atacctgaac catcccacgg cagtacccgg
    17641 acaggtcatc ggaagggcgg acggtctggc ctccaaaaac cgttttttcc attggccact
    17701 ggagtttccc gaagtatttg accttgcctc cggaagcagc ggttttgacg tgatggtggg
    17761 caatcctccc tgggaaagga taaaactcca gcagcaggag ttttttgcca tcagagacag
    17821 gaatattgcc aatgccccca atgcggcagc ccgaaaccgg atgatcgatg aactccagat
    17881 taccaatcct tcactctggc gtgaatacca ggaggcgctg catgccgccg actccaccgg
    17941 aaaattcctg cggaacagcg gaaggaacga cctgaccgcc gtgggagata tcaataccta
    18001 ttcggttttc gcggaactct tctcttccat gatcagccag cgcgggaaaa caggatttat
    18061 tgttcccacc ggtatcgcca ctgatgacgg caacaaagcc tttttcgggg cgatggtgga
    18121 aagaaacagg ttggtcagtt tatttgattt tgagaatagg gaagccattt tcccgggtgt
    18181 tcacagaagt tacaagtttt gcctgctaac ccttcagggc agtgaccggg gaaaggagaa
    18241 agccagtttc gggtttttcc tgacccgggt ggagcatttg caggacaaac tcagggtttt
    18301 cagtctgagc cgggaggatt tcctgcggct gaatcccaac accaagacct gtccggtttt
    18361 ccgcaccagt gtagatgccg ccctcaccac cgatatctac cgccgtgttc cggtcttgat
    18421 aaatgaaacc accggggaga atccctgggg agtaagtttc atgcggatgt ttgacatgag
    18481 caacgactcc catctttttc gcacccgtcc ccagctggaa gccgaaggct ttcacctttg
    18541 gggtaataaa atagtgaaaa aagagccatt gccgaatggt gaatggcgat tagaaaagat
    18601 gtggttacct ctctatgaag gaaaaatgtt tatgcctttt gaccatagat atgcaaatgt
    18661 agtaattacg gataatcctc ttagggcggg acaaccagag tttgctcaat attcagatca
    18721 tataaatcct aacttcacaa acgaacccag atattgggtt gaccttaatg aagtattgag
    18781 aattaaaact gattcatatt ttcttggctt caaaaatgta acttctggga cgaatgaacg
    18841 gactttttta gctacaattc tgccaaaatc tgcgattggc cattcaactc caatcatatt
    18901 tgtaaacaaa gaaactttgc ttaaatccgc ctttttagga aattgtaatt caatttcctt
    18961 tgactacttt gcaaggcaaa aattaggggg aataaacctc acgtatggat atgtccagca
    19021 atttccggtt attccaacaa ttgattctac agatgctatt ttagttgaaa tcactcctaa
    19081 gcttattgaa ctcatttact cttcctggga catcaaatcc tttgctgatg atgtctggaa
    19141 agaggcagat gaagatctga aagctgccat ccgggagcaa tgggaggaga acaaagcagc
    19201 cacaggcgga catgaatggg atcccccgga atggtgcgaa atcgatccgg agggatgtcc
    19261 ccttccacct ttcaaatggg acgaagagcg ccgtgccatt ttgaaagccg aacttgatgc
    19321 catttatgca cgcttgtacg gtctcaaccg caagcaactg cgctatatct tagatcccgc
    19381 cgatcttact ccccgggagc tggaaaacat cctcgatccc tgggaggaag tcactaatcc
    19441 cctcgatccc gaaggctacg cccaccgttc cgcccaaagc accttccccg gcgaaacctt
    19501 ccgggtcctt aaagacaagg acatccgtca gttcggcgaa taccgcaccc gcaggctcgt
    19561 ccttgaagcg tgggaaaggc agaacaaaag ctaatcacct ggagtgtaaa aaaagcaaaa
    19621 cagaataata ggaggaacat aactcttctc tgacaaaaaa gaaatgcaag tttcactttt
    19681 aagtgaaata attcattatt ttcgctagcc caaattgaat gaattacaaa cttgtaaaaa
    19741 tagtcgaact aagtggagat aaggcttctg tttattcttt atatacagaa gatgagaaat
    19801 gcacgctgtt tgaaaagttt atgagagaaa attcaaaatc atttaaaagt gaaacaattg
    19861 atatactaaa acgtttaaat agtattgcgt cgaaagaagg ggcaagggaa caatatttta
    19921 aaaccaggga gggaaagccg ggagatttgg tatgtgcgct ctatgatagg cccaatagcc
    19981 acttaagact ttattgtata cgcctgggag gttcactaat tattcttggg ggcggagggc
    20041 acaagcccaa aacgataagg gcattacagt ctgacccgaa attaagttct gaaaatagcc
    20101 tgatggttaa attatctggc gaattattgc atagggtaaa aagtaaagag atcttatacg
    20161 tgaatgatta tatggatctg gaaggtgacc tggattttga attttgaaag aacaataaaa
    20221 gaccactatg aaaaaagcag aaagttattc cagcaactta ttaagggaat ttctgggaga
    20281 tatttcacta aaagagcagg aaaaaaccga taaaaggatg agacttgcag cagttatcga
    20341 caatggaatc aaagccaaag ggtggaaaaa gtcagacctt gctaaagcat tgaacaaacg
    20401 tccttcggaa atttccaaat ggttgagcgg tacacacaat ttcaactcag acaccctgtt
    20461 cgacattgaa gaagcattga atatcaggct tataaattta tttgaagagc ctaaagtgca
    20521 ggttacccgc tacaatttat tcgtcagttc ccgggttcct acctcagcag gtgtattaga
    20581 gaactcttcc ccattaggaa atttacttat cgcgcaggat cccactcaga tctattcttc
    20641 caggagtacc attgatcaaa gaactcaaac tgacttccgg gcatgaataa tatgacgaac
    20701 gcaacaaaag tgacttttca gattaaaggg attgatctgg ttgacattca aatgaatcaa
    20761 ctaaaacaac cattaccggc gcagacaact tttcatttca acatcaacct ggaacaattg
    20821 ataaatcccg aaaataagct ggtgatagta gtagcaacgg ttgtgattat tcacgaggac
    20881 aagacgacca ttctcgcatc aataaaagcc agttgtgttt ttgatgttgt caatttcgac
    20941 gagtttgttg cagaagacac caggaaagtc acttttcctg aaagtgcagt tatcactttc
    21001 aactccatca ccatctcaac ggtcaggggt ttgatgtttt cccaattcaa aggcacacat
    21061 ctccatactg caatcttacc ggttgtagat ccttcttcat tcattaagaa ttgaattgat
    21121 gaataggcaa acacaagaat tctaactatt cccagaaact cgtttcggtt tttcagtaga
    21181 attcaacatt ccaacactaa atagatgctt tcgcgatgta gaccagaaat atataaaatc
    21241 gatcgagaaa gatcttcccc aaatgatagt tccaatcaat aaaaacacta ttttgctatt
    21301 aagttcaaat caatctaagt ggtactgtat aaccccatga gacagtagca cgttttgtcc
    21361 ggaatatttt gtaaacgaac ccaataaaat attaatcaaa tggcaattat actacaaaga
    21421 caaatttcag aggacgagaa gaaacaaatt attgaaagat ttgggcgtaa gtgctacgca
    21481 actggacatg aaataccaat aactgaagag atacattatg atcacatcat ggcattttca
    21541 gatgatggtg catccgagat tgataacata gctccgatgt gccagatgca taatcttcaa
    21601 aagggtagac tgccactaca agattttaga ataaaactca gacttgatga ttttttcaaa
    21661 catgggcagt cccatacttt aaagcatgaa ctccagcatt tcaaagatca aggaataatc
    21721 aataattttg gtgaaaacgt ttattctatc attacagata accatattga acttgagttt
    21781 ttgaacaaaa aagagacttt taggctttat tcttgcccaa taacaaaatg gaaatatttt
    21841 tatgcaactt tacctgttga tgtaattaat agtgatgatg atgaagatgg caaaatagga
    21901 cttcagccac ggtatctaat acaagataaa gtttttgatc tttatagaca ttttcaacgt
    21961 aatactgttt tgaatccttc aattggcaga ttgcataata ataaaatcct catatttgat
    22021 ggtcagcata aaattgcttc attattatgg ggagaacgaa aatattttga acttaagatt
    22081 tatattaatc ctgacccaaa aagtctaaat caaacgaata tttctgccca tgataaattt
    22141 gcccaaacaa gattctttgc ctctattatg gtagcaaagc ttggaagtca atttggtaaa
    22201 caatttgatg aatacaaaaa cctcgaagat ggtgtgaaga aaacagaagc aggatttgtg
    22261 aaatttgtaa aaaacagtga acagttatct aatgccgata taaacaagcg attcaccagc
    22321 ttcatttatg attcaattct tgacgagtca acaaataaga tagtaagact tgtttctaaa
    22381 tccaatagaa gtacaatgga atatccaatt acaatggata tgttgagtaa atcaatcttt
    22441 tccaattttt tatatagaaa cccaactgaa gatgacttaa caagcgatag ctataaaagg
    22501 gataaagaaa tttcaaatgc gattaggcta tgcaacatat tagatgaaga aattttacac
    22561 aaatgggatg caactaaaac ggaaaacgac ttggtgcaaa ataaattaaa tcgaatgttt
    22621 cgaagcaaat cgattatggc ctgggcagaa ttattaagag atgctattgc agcaaaactt
    22681 gaaattacag atcaagatga aaaagcacaa attttttata gagacattac agatgaaagt
    22741 tttgacaaag ttaaaaaggt tgttagaaga ttagtttcat ggagtgtgtg ggattcaccc
    22801 gttaattcag aaattgatag gatttttgca gataataaga gcgaggtaaa aaaatatttt
    22861 aaagataagg gattaacaac tgggtattta ttaggagcaa ctgaatagat tcaaatagac
    22921 cttactcata acgaatgtaa ttactagaat tgctttaaat tttagcttta ggattttcac
    22981 ttagcaatcc ggtcgcaccc tagtacaaag ctttaacttt tttgggattc ttgcagtaag
    23041 gttgaggtct ttccataatg tttgatctta aaattatgaa catttctagg ttcaactttt
    23101 cacgtgggtc ctgatttttt cgaggcattt gtatttctgg ttgcgggcgg aggcggcatc
    23161 cttgtaaccc agcttcgagg caatgaactc ccagtccttc tgccggtagt aaaaccactc
    23221 caggagcgtc t
//
LOCUS       DAOTHZ010000032        23193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2603_length_23193_cov_7.516639, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000032 DAOTHZ010000000
VERSION     DAOTHZ010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 23193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23193
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2603_length_23193_cov_7.516639"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="P5549_05365"
     CDS             <1..192
                     /locus_tag="P5549_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12533.1"
                     /translation="AMLVCAHIAARHLFAGRLSIGLYGLRQVNNTKKPLQGWKNARII
                     VNAERNRACQGKRVAKQHS"
     gene            173..466
                     /locus_tag="P5549_05370"
     CDS             173..466
                     /locus_tag="P5549_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRW12534.1"
                     /translation="MQNNIHKISKVKLVVGKFSMALPDSLQFAFAAIAAVEPLLEAAV
                     LEIELRSVICRCLECNIRTEIDENYSFICSNCASVSLEIIQGRELYLDYYEGE"
     gene            472..1131
                     /gene="hypB"
                     /locus_tag="P5549_05375"
     CDS             472..1131
                     /gene="hypB"
                     /locus_tag="P5549_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRW12535.1"
                     /translation="MVQVRMESNLLGTNDLLARNNRRNFARRRLLVINLMSSPGSGKT
                     TILEKTIENLNGKLNLGVIEGDLYTDRDAQRIEQKGVKVVQINTEGACHLDAGMVDKA
                     FHNLPDGPLELLFIENVGNLVCPAEFDLGEDYKAVVMSTTEGNDKPVKYPLIFREAKV
                     VLLNKIDLLPYTNFSLQQFREDLAQINPMASVFYISGRTGEGISEWNDWLLEEVRSKR
                     A"
     gene            1256..2314
                     /locus_tag="P5549_05380"
     CDS             1256..2314
                     /locus_tag="P5549_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRW12536.1"
                     /translation="MPKKLSRRDFVKICAGSAAAISMSGFLAPYIAQAAEAGAPPVIW
                     IQGASCSGCSVSLLNTVHPDIKDVLLKTISVRYHPTLSAAAGDLAIEEGIFKTVKENP
                     KKFYLVVEGAVPTAADGMFCIVGEREGKPLTFLQMVQELGSQAAAVLNFGTCSAYGGL
                     PATPPNPTGCKAVEDVVKNVPIVNVPGCPPHPDWMVGTIAHVLLYKDIPELDTFKRPK
                     MFFGNIIHDNCQRRQYFDNAIFAKNFGDPGCLLELGCKGPIAHCDSTTRLWNGGVNWC
                     VKAGAPCIACTEPQFPGWPIYERMPEMPIGPAMTATVDQIGGILGAAAVLGVGGHLAG
                     NIMTGRIGPKKDEKEGDN"
     gene            2314..2505
                     /locus_tag="P5549_05385"
     CDS             2314..2505
                     /locus_tag="P5549_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRW12537.1"
                     /translation="MAERVIIDPVTRIEGHLKVEVEVEGNSVVNAQASGMLFRGLEII
                     MRGRDPRDAQQIMQRVCGV"
     gene            2419..3753
                     /locus_tag="P5549_05390"
     CDS             2419..3753
                     /locus_tag="P5549_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2503..2505,aa:Sec)
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRW12538.1"
                     /translation="MLFRGLEIIMRGRDPRDAQQIMQRVCGVUPVAHASAGSLALDSA
                     FGIEPPPNGRIIRNLILGSNYIQSHILHFFHLAALDFVIGPDVPPFVPRYQGDYRLSA
                     DVNELAVNSYLEALNMRRKAHEMTALWGGKMPHQQAIVPGGVTEVPDTSKIFEFVSRL
                     DELTAFIDNTYIPVVKAVAGAYADWFDIGRGCMNMLAYGAFPLEEGKDHVKKKKFFPA
                     GVYERGQYKDLEPEKITEQVKYSWFKDGIAVTPDQAVVEPDTAKKDAYSFIKAPRYNG
                     EVMEVGPLARLIVSKQADVMALGDKAFSVMGRHFARAVECLEIAHAMKDWVLKLEAGQ
                     PVATPYKIPASAQGMGLTEAPRGALGHWNHIEHQRTAVYNAIVPSTWNMSPRDDNNKM
                     GAMEQALIGTPIADAKNPIEVVRVVRSFDPCLACAVHIMTPDKKVISQFQIN"
     gene            3765..4412
                     /gene="cybH"
                     /locus_tag="P5549_05395"
     CDS             3765..4412
                     /gene="cybH"
                     /locus_tag="P5549_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HRW12539.1"
                     /translation="MVKEMEVRHSVWIRFFHWTNMIAITLLILTGYYIHSPHGFRLFD
                     NMDTPRMIHFAMAYVLCFGVVGRVYYAIAAKDAKNIVFSVKEDTPKFPSMIKYYLFLA
                     NTHPYYGKYNPGQKMMYTGWLFMALIQIITGFILYKPHTFAWLGGALGGLIAIRVIHY
                     IVTWLFVLSVLAHVYLDVSEGIPVLKSMFTGKIPADFDHGVHEEHEAEVGNGISA"
     gene            4430..4894
                     /locus_tag="P5549_05400"
     CDS             4430..4894
                     /locus_tag="P5549_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HRW12540.1"
                     /translation="MHKLMVVGIGNYILQDEGVGVHAINRLMKMDLPSDVELLDGGTH
                     SYDLVDFFCQAEKLIIIDAIQAGGEPGTMYRAPLEEMGLQPQENCTSLHQMHFIEAVK
                     MVNLLGHYPQIIVYGIEPEVVDWGLELTPRVAEKLPRLTEMIAQEISDLMAH"
     gene            5050..6645
                     /gene="hypF"
                     /locus_tag="P5549_05405"
     CDS             5050..6645
                     /gene="hypF"
                     /locus_tag="P5549_05405"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRW12541.1"
                     /translation="MNVKMNSYRIKINGIVQGVGFRPHVYRLAMRYELKGWVLNSSAG
                     VIIEIEGEENIIRQFLHQVVEEPPALAVIRDWQIDATPWHGYQEFTIKISDDREDKTV
                     MISPDIAVCKECREEVMDRQDRRFDYPFTNCTNCGPRFTIIKGLPYDRAMTTMDAFPM
                     CPACQAEYENPLHRRFHAQPNACPVCGPHTTLCDNRGKSVNGDARDYLKQGHIVAVKG
                     LGGFHLAVDACNRDAVANLRKRKKRDAKPFAVMARDLEAAHKYCQINAEEEKWLASPQ
                     APIVILERNEQCLLAADIIHPGIKTLGVMLPYTPLHFLLFDEELEILIMTSANISDEP
                     LIIDNHEALDKLKGIADCFLLHNRDIYNPCDDSVMRVTSFNTPHLFRRARGFVPRGIP
                     LNIKTEPVLALGGEMKNAFCITRQGEAFLSQHWGDLNHYLNYVNFQRGIERFKQSLYV
                     EPKILAHDLHPEYQTTRWARQQPYPKKIAIQHHFAHMASVMAEHQLQGEVLGLICDGT
                     GWGTDGAVWGGRFCKETVAISSG"
     gene            6705..7319
                     /locus_tag="P5549_05410"
     CDS             6705..7319
                     /locus_tag="P5549_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12542.1"
                     /translation="MSLIYLSAALGEKGLAHAEKILPDLSGQELAFMLQQMKTNHSVS
                     QTSSCGRLFDAVAAVLGVCGINRYEGQAAAELEALADQSEKGRYGFTLCKEQNIWLMD
                     VLPMWPELIADLQQGRRRASMAQKFHLTLVEMFVTTLLRLRDESGLTRVVLSGGVFHN
                     QILLYHLVEQLTEKEFKVFYPRQVPAGDGGLALGQAIIASEVLA"
     gene            7319..7543
                     /locus_tag="P5549_05415"
     CDS             7319..7543
                     /locus_tag="P5549_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRW12543.1"
                     /translation="MCLGVPARVVEKTTNYSARVDVNGNHTEINIQLTPDVEPGQFVL
                     VHAGFAMEIIDESIALETMHYLEEMLQYGQ"
     gene            7533..8615
                     /gene="hypD"
                     /locus_tag="P5549_05420"
     CDS             7533..8615
                     /gene="hypD"
                     /locus_tag="P5549_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRW12544.1"
                     /translation="MDNDVKEYKQHLLRAIQLFSGSVSLMEVCGTHTMAIAKSGIRGL
                     LPPNIKLLSGPGCPVCVTSQGDIDAVIVMVQHKDIILITFGDMMRVPGTHSSLQEERS
                     RGADIRIAYSPLDALRVARDNPDKEVVFLGIGFETTTPTIAITIEQARREGLNNFSVF
                     SLHKVVPPALELIFSDSEIKVDGLLCPGHVSAVIGVKTFGSLARKYHKPCVVTGFETL
                     DILEGIYMLLQQLKKEEARVENQYSRVVKPEGNPIACQSMERVFQPVPARWRGLGMIP
                     ASGLQLREEFSDMDARKRFDIPEIEEIPIKGCACGEVLAGKITPLECVLFGQACTPLN
                     PVGPCMVSHEGSCAAYYRYIPTKGRS"
     gene            8616..9626
                     /gene="hypE"
                     /locus_tag="P5549_05425"
     CDS             8616..9626
                     /gene="hypE"
                     /locus_tag="P5549_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRW12545.1"
                     /translation="MTQERILLAHGSGGLMSQRLIDEVFKQAWRNEIMEAMLDGAILQ
                     VPAGRLAMSTDSFVITPLFFPGGDIGKLSICGTVNDLLACGATPLYLSTAFIIEEGLP
                     LADLRVIAASMAQTAREVGVKLVTGDTKVVEKGSADGVFINTTGIGIIPADIDYRPQR
                     IQPQDQVIVTGCVGDHGLAILARREGLAFSTPVVSDCAALIQLREIISQYGNAIRCMR
                     DPTRGGLATVLNEIAVQAGVGIHVKEADIPVAPAVQGACDLLGMDPLYMANEGKMVIF
                     VAAALAQSLLNDLRRLPQTQAASIIGQISAETPGMVLIETELGAQRILGILEGEHVPR
                     IC"
     gene            9688..11922
                     /locus_tag="P5549_05430"
     CDS             9688..11922
                     /locus_tag="P5549_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12546.1"
                     /translation="MHIRNIFQRLLIPVFITLFALSAFPPLVQAASSEGGPTAYIILI
                     DKLSINDIDPVATPAMYRLTQQGAVGLASNRTLRGHNTIDCSLTLGAGNLARGYLNGL
                     MGFNQNEIVPERNKTAGQLYQNLTGISGADSALLMVNYPEILEGMIQESVSTIPGAMG
                     EVLRANQLKVCVLGNGDIGNERIRPGIAVGMDARGQVPLGEVGSAVSSLSPASFLSHE
                     TNYEFISEQLEQNRSQADLIIIDLSDLARFQEGDTALPDIYTFQKKSIMRKMDYIVDQ
                     IMQQLDPQKDLLLLSGISPSREAIKLKDNFTPVLAYGKGFSAGILTSSTSRRDYIVAN
                     TDLAPTVLKFFELKDEQRVMIGQVMFSKPEAEGDRLAAAIDLAGSTSTANRLRSPVIK
                     SYVVLQIIVILLALLAIFPLKQGKPIIKSLIVSMVAIPLVLLPLNKIPLHDDWLYVAA
                     AILLTAGVTKLALYFCKDNAFKSLMMISLVTVLMINLDIIMGAPLIKSSLLGYDPMTG
                     ARYYGIGNEYMGILIGSSIAVGTALYEKYKHRLLLFIIAVFFTSQCYLIAGPTLGANS
                     DGLLTAPVAFLVTFLLLRQVKISFRAVVGIFGAVLMVAAGGTMYDMQRPAELQTHIGR
                     AANQIAAGGWQEILLIIKRKVGMNIKLIRYTIWSYVFMVILLVLSLLLVHPIGAMRQL
                     RQQYPAVFNGFIGIITAALVALLVNDSGIVAASTTSIYLVFPILLLIMDIDQDIPDTR
                     AEEN"
     gene            complement(12003..13730)
                     /locus_tag="P5549_05435"
     CDS             complement(12003..13730)
                     /locus_tag="P5549_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRW12547.1"
                     /translation="MSETTVMINGIPISVPEGTSILHTAQKAGIKIPTLCFHPDQEVK
                     ANCRICVVEVEGQKNLMPACATKVNEGMTIKTATPRVLEARRTILELILAHHPQDCLH
                     CVRSGSCELQEIASEYDVRYNRFPQVEADLLLDYSSPSIVRDPNKCILCGRCIEFCTS
                     KQTVNALGLAHRGFDAQVVPSIGQELFESPCVMCGQCIHACPVGAISGREQIDEFMTV
                     AADPEKIVVTQIAPAVRLAIAEAFGADRDQLQMGQLVSALKMLGFDHILNTNFAADLT
                     IMEESQELLERIRNGGVLPMFTSCCPAWVRFAEVFYPDYLNHLSTCKSPQQMFGALVK
                     TYWAEKMSIHPDKIFSVSIMPCTAKKYEAVRPEMTDSDYSDVDMVLTTREIVRLLQMT
                     GINPLKLKETAFDPWMGEYTGSAVLFGASGGVMEAALRTVYEKVTGRVLEELDFTDVR
                     GMDSLKEATIDLDGTIVKVAAAHTLGNARALMDEVAAGTSPYHFIEIMACPGGCIGGG
                     GQPITRSNVQREQRMEAIYVEDRSKTLRKSHDNHEIQMLYTDFLQETLGHRSHELLHT
                     QYSLKRKNP"
     gene            complement(13755..15611)
                     /gene="nuoF"
                     /locus_tag="P5549_05440"
     CDS             complement(13755..15611)
                     /gene="nuoF"
                     /locus_tag="P5549_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRW12548.1"
                     /translation="MHSLNEMRHQFRSAYEESQHHIKVVIGLGTCGIAAGGEKIWEAV
                     SRYIETHGAEVEMMSTGCIGMCYAEPLMEVHMPGQPRFIYGNVKPEAAVAILQKHLAE
                     GKPSPEYIIAQDSRGYEAAANIVKLEETPFYQGQVKNVLGRCGIIDPDQIVQYITFSG
                     YEALEKVLSSLTPFEVIQEISKSGLRGRGGGGFPAGRKWEASYHSPGSKKYVVCNADE
                     GDPGAFMDRSVLEGDPHSLLEGMAICGYAIGADEGYIYVRAEYPLAIKRLKKAIRDAE
                     SMGLLGNNILGSGFSFKIQIAAGAGAFVCGEETALMHSIEGKRGMPRVRPPYPAQSGL
                     WGKPTNINNVETYANVPGIIRQGAAWFASMGTENSKGSKVFALTGKINRTGLAEVPIG
                     ISLRDIVFKVGGGIQNGKRYKAVQIGGPSGGCIPESLLDTPVDYDSLLQLGAMMGSGG
                     LVVMDEDTCMVDVARYFLHFTQEESCGKCAPCREGTMRMLQILEKICDGKGTPTDLDT
                     LESLCRVVQSTSLCGLGQSAPNPVLATLRYFRSEYEAHIYDKKCPAGVCVELLDIKID
                     ADKCKGCTLCADVCPVGAISGERKKPHVIDADKCIKCQACMVKCKFGAIAKG"
     gene            complement(15599..16114)
                     /gene="nuoE"
                     /locus_tag="P5549_05445"
     CDS             complement(15599..16114)
                     /gene="nuoE"
                     /locus_tag="P5549_05445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRW12549.1"
                     /translation="MCDCSEHVQDMRYVALDKMLDKYRDSNGKIIPVLQETQDIFGYI
                     PEDAIKTIAETLRIPSSEVYGVATFYGQFHLQVRGKNIVRVCTGTACHVQGSGKILEK
                     INKSLGLSAGKMTTDDLLFTVEPVACLGACGMAPVIMINEDAYGRVSPDDVPEILARY
                     QEKDGEVKCIA"
     gene            complement(16215..16751)
                     /locus_tag="P5549_05450"
     CDS             complement(16215..16751)
                     /locus_tag="P5549_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12550.1"
                     /translation="MGTAKAIKINYHTKAAYEMASSLPCPRSAHEVYNVGVSLQYCIR
                     AKYLEIAELLNDKTYLTDQAAQQLVIKSKIAKLAAFRLNVQLNKFYELGGPIMEDPVS
                     KEMAKDIQPFFKRIMSRFLQSLDESSSRLLTKQISTEEMTAEVKQQITDVYTVMEKLF
                     PVDEITSAFDELIEVLEA"
     gene            17225..18562
                     /locus_tag="P5549_05455"
     CDS             17225..18562
                     /locus_tag="P5549_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12551.1"
                     /translation="MRFMQEYKNKLVTAKKAVKTVKSGDRIQFNSYNGNPPVLDRALA
                     ARRDELEDVVLYTSVTMYPLQTIACDPEGRHFIYNNMHASGHDRKLALAGNYYYAPVT
                     YYELPTILKNERRDVVMLQVGSMDDMGYMNHGPNAGHAQDLVRNANLVIVEVNRNMPT
                     AYGGHDAAVHISEVDLVVEGDHQPLFTLPALQPNEVEKQIARWVMTEIEDGACIQLGI
                     GAMPNFIGELIVESDLKNLGCHTEMLADAYLKIYASGKMNGRNKGIDKGKMVYGFAMG
                     SQDLYDFIDQNPVCATYSIADVNTPHVVARNPKMVAINGAIEVDLFSQINSESSGTRQ
                     ISGTGGQLDFMMGAFMSEGGKGFICLSSTYTNQEGKLCSRIRPTLSPGTIVTTPRSLA
                     HYIVTEYGIAYVKGKSTWERAEALIKIAHPRFQDELIKEAQEMNIWRRSNRVN"
     gene            complement(18584..19045)
                     /locus_tag="P5549_05460"
     CDS             complement(18584..19045)
                     /locus_tag="P5549_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRW12552.1"
                     /translation="MQDVSGFDIDHTVMGTLGIEIFEYTPERVVARMPVTPKVHQPFG
                     LLHGGVSLVIAETVASAGSYQFIDASWQRAVGLEINGNHIRSKASGIVHAVGTPIHVG
                     RRTIIWDIRIYDEEETLICISRCTMAIIEVGPQPAEKRKTDPFSFQEDVSQ"
     gene            19440..20486
                     /locus_tag="P5549_05465"
     CDS             19440..20486
                     /locus_tag="P5549_05465"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRW12553.1"
                     /translation="MAHLYTSDEQIDRLVDFKGAREYHLTKDRRKKDKRMPLKEAIAE
                     FVHDGDIFAETGFSYVRSPIQAYFEIVRQQKKNLTGIGSPMSNCSYFMARGCMKAIHL
                     SYIGVEMRGNDKNFARIIKNKEAEILSFWSHGTMAQGFKAAQLGAPFIASKQLLGTDM
                     IKQNPYVKVIDNPVGIDHDPVCLIPALFPDVCIIHVQQADRFGNAVISGPAINDIALA
                     AACRRVIITAERIVPEMTIRDNGNASIPFMYVDAVVELPYGAYPGCCPGHYFWNREWW
                     EFLIRIGTVTYDNMIDFTDRWIMNTKDQYDFIDKAGGMAVIDKARRLMEAAQGTADDT
                     AISFDYQEVIPKWD"
     gene            20502..21284
                     /locus_tag="P5549_05470"
     CDS             20502..21284
                     /locus_tag="P5549_05470"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRW12554.1"
                     /translation="MSNVAPANPLEMLAYNLSMMIEDNQIVYVGTGLPMVGGMLAKKT
                     HAKNITLVFESGGQDPLEGCDVPWSVGCPFTYRKSPLTMEMSISFGQCALGYVDIAFQ
                     GGAQFDMYGNVNTSVIGSYENLKSILPGSGGGNDIGSLTEKSVLVGLQLPERFPERVD
                     FLTTPGYLEGGDSREKAGLYGGPIAVVTQVGIYDFEPESKRMRIKALNPGMTVEIAQM
                     CTGFELLVPEEIHMVAPPPDEVLEILRTVVDPRGVFTKMPGM"
     gene            complement(21366..22157)
                     /locus_tag="P5549_05475"
     CDS             complement(21366..22157)
                     /locus_tag="P5549_05475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRW12555.1"
                     /translation="MNTPAPANPFEQLAYNIALQIHDGMIVYVGTGLPMVGAMLAKKT
                     HAPHITLVYESGGQDPWEGPMPWSVGGAATFRRAPMSMEMAHSFAMAANGFVDIAFQG
                     AAQIDMYGNLNTHMIGGDFHNFKSRLTGSGGGNDLSSLSETFVLVGLQDPSKFPAKLD
                     FMTSPGFLSGGNSRIEAGLLGKGPQSVVTQIGVMDFEPESKRMRVKGLNPYCTVDIAQ
                     QCSGFELLVPEKVEEIPAPPSDILEVLRQEVDPHKIFIEFPPRVK"
     gene            complement(22184..>23193)
                     /locus_tag="P5549_05480"
     CDS             complement(22184..>23193)
                     /locus_tag="P5549_05480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRW12556.1"
                     /translation="HESFRETNLKKSRLKEDKRMSLKDAIAKYVEDGCIMADSGFSYV
                     RTPIQAHFEILRQNKKNLQYIGSPNTLHSFAVFAGCCNWTHNSYCGAEMRGTDRQFSK
                     GVKEGKTQIISEWSHGSMALGFKAAQYGSPYLAAHQLLGTDIVKYNPYVKEVKDPWSG
                     QPVVLIPALHPDVCIIHCQQADIFGNGKIVGPAVNDIALAFASRKVIITCEEVVPELS
                     IRFDNSNTVIPFMIVDAVVELPYGCLPGSCPGHYYWAREWWEWLMRIATKNDVAMSDF
                     MKNWIYDCPDQYALVDRLAKDLGGFSYIDNLRRLEKAEEYLIEDIGVDFTYETVIPKW
                     E"
BASE COUNT         5974 a         5331 c         5884 g         6004 t
ORIGIN      
        1 gcaatgctcg tgtgtgctca tattgccgcc cggcatcttt ttgcggggcg gctttctatt
       61 ggtttgtatg gcttgcgaca ggtcaataat actaaaaaac cattacaagg atggaaaaat
      121 gcacgaatta tcgttaatgc agagcgtaac agagcttgtc aaggaaagcg cgttgcaaaa
      181 caacattcat aaaatcagta aagtcaagct ggtcgtaggc aaattttcaa tggctttgcc
      241 cgatagcctg caatttgctt ttgcagcaat tgcagctgtc gaaccattac ttgaagccgc
      301 tgtcttggag atcgaactcc gttccgtcat ttgccggtgc ctggaatgca acatcaggac
      361 agaaatagat gaaaactatt cttttatttg ttccaattgc gcttcagtaa gtctggaaat
      421 catccagggg cgggaattat acctggatta ctatgaagga gaataaagat aatggtacag
      481 gttcgcatgg aaagcaattt attgggaacc aacgatttat tggccaggaa caaccgccgg
      541 aactttgcac ggcggcgttt gctggtgatt aacctgatga gttcgccggg atccggcaaa
      601 accaccattt tagaaaaaac gattgaaaac ctgaacggaa aactaaacct gggtgtcatc
      661 gaaggagatc tttacacgga cagggatgcc caacggatag agcaaaaagg agtgaaagtc
      721 gtacaaatca ataccgaagg tgcctgtcac ctggacgccg ggatggtaga caaggctttt
      781 cacaatttgc cggatggccc gttggaattg ctctttattg aaaatgtcgg caatttggta
      841 tgtccggcag agtttgattt gggggaggac tataaagcag tcgtaatgag tacaaccgaa
      901 ggcaatgaca aaccagtcaa atatccgttg atttttcgcg aggcgaaggt ggttttgttg
      961 aacaaaatcg atcttttacc ttatacgaac ttcagcttac agcagttccg ggaagatttg
     1021 gcgcagatta acccgatggc gtcggtgttt tatatatccg gacgtaccgg tgaaggaatt
     1081 tctgaatgga atgattggct attggaggag gtgagaagca aacgagctta atcgtagaaa
     1141 gatagagaat aagctctaac aattatttaa acaggttagg taatggtttt cataatcaca
     1201 acgattggaa ttggatggga ttattttatt aaatatgttg gggggaaaag gtcagatgcc
     1261 aaaaaaacta agtcggcgtg attttgtcaa gatctgtgcc ggatcagccg ctgccatttc
     1321 catgtcggga tttctggcac cgtatatagc tcaggcagca gaagccggtg cgccgccggt
     1381 aatttggatt cagggtgcga gttgttcagg gtgttccgtc tcactactga atacagttca
     1441 tcctgatata aaagatgtcc tgctcaagac cattagtgtt cgttatcatc cgactctttc
     1501 ggctgcagca ggtgatttgg caatagagga aggaatcttt aagactgtta aagaaaatcc
     1561 caaaaaattc tatcttgtcg tcgaaggggc tgttccaact gctgcagatg gtatgttctg
     1621 cattgtagga gaacgtgaag gcaagccgct aactttcctg caaatggttc aggaactcgg
     1681 cagccaggcg gctgctgtgc ttaattttgg tacatgttct gcctatggag gattgccggc
     1741 aacgccgccc aatccaaccg gttgcaaagc ggtggaagat gttgtcaaga atgtaccgat
     1801 cgtcaatgtt cccgggtgcc cgccccatcc agattggatg gtgggaacga ttgcccatgt
     1861 gcttttatat aaggatattc cggaactgga tacctttaaa cgccccaaga tgtttttcgg
     1921 caatatcatt catgacaatt gccagagaag acaatacttt gacaatgcca tttttgccaa
     1981 aaactttggc gaccccggtt gtctgcttga attaggctgc aaaggaccga ttgcccattg
     2041 tgactcaacc actcgtttat ggaacggcgg cgtgaattgg tgcgtcaagg caggtgcccc
     2101 ctgtatagca tgtactgaac cgcagttccc aggctggcca atctatgaaa gaatgcctga
     2161 aatgccaatt ggacctgcca tgactgctac cgttgaccaa atcggaggaa ttttgggagc
     2221 ggctgcggta ttaggtgtcg gcggtcatct ggccggcaat atcatgactg gtcgtatagg
     2281 tcccaaaaag gacgagaagg aaggtgacaa ttaatggctg aaagagtaat tatcgatccc
     2341 gtaaccagaa tcgagggaca tctgaaagtg gaagtcgagg tggaaggcaa ttctgttgtc
     2401 aatgcccaag ccagcggcat gctgtttaga ggcttggaaa tcattatgcg cggtcgtgat
     2461 cctcgcgatg cgcaacaaat aatgcagcgt gtctgcgggg tttgaccggt tgcacatgcc
     2521 agcgcgggtt cgctggcttt ggacagtgca tttggcattg aaccgccgcc caatggtcgc
     2581 attataagaa atcttatcct cggttccaac tatatacaat cgcatatcct gcattttttc
     2641 catctggcag cgttggactt tgtaataggt cctgatgtac caccatttgt gccacgctat
     2701 cagggcgatt atcgtctgtc cgccgatgtt aacgaactag cggtgaacag ctatctggaa
     2761 gctttaaata tgcgtcgcaa ggcacatgag atgaccgccc tctggggagg caagatgcct
     2821 caccagcaag ccatcgttcc cggcggggtg accgaagttc cagatacctc aaaaattttt
     2881 gagtttgtca gccgtttgga tgagctgact gcttttattg acaatactta tattcctgtt
     2941 gtcaaagcag tggcaggagc ttatgcagat tggtttgata ttggccgggg ctgcatgaat
     3001 atgttggcat atggtgcttt ccccttggaa gaaggcaagg accatgtcaa aaagaagaaa
     3061 ttcttccccg ccggtgttta tgaacggggc caatataaag atttggagcc tgaaaaaatt
     3121 accgaacagg tcaagtattc atggtttaag gatggcattg ccgttacacc ggatcaagcg
     3181 gtggttgaac ctgatacagc caaaaaagat gcatactctt tcataaaagc accccgttat
     3241 aacggcgaag ttatggaggt tggtcctctg gcacggttga tcgtaagcaa acaagctgat
     3301 gttatggcat tgggtgacaa ggctttctcc gtaatgggac gtcattttgc ccgcgcggtt
     3361 gaatgtttgg agatagcaca tgccatgaaa gactgggtgc ttaaactgga ggccggccag
     3421 ccggtggcaa ctccctataa gattcctgct tctgcacagg gcatgggact aacggaagca
     3481 ccacgtggtg cactaggtca ctggaaccat attgaacacc agagaactgc agtttacaat
     3541 gcaattgtac ccagtacctg gaatatgtcg ccacgtgatg ataataacaa aatgggtgca
     3601 atggagcagg ctttgatagg aaccccaatt gctgatgcca aaaatcctat tgaagtggta
     3661 cgggtagtca ggtcatttga tccctgtctg gcttgtgctg ttcatattat gacgccggat
     3721 aaaaaggtga tcagccaatt ccagatcaat tagggggtga gaaagtggtt aaagagatgg
     3781 aggtaagaca ttctgtctgg attagattct ttcattggac caatatgata gcgatcaccc
     3841 tgctgatttt gactggatac tatattcaca gcccgcatgg tttcaggctg tttgacaaca
     3901 tggacacgcc ccgcatgatc cattttgcca tggcttatgt gttatgcttt ggcgttgtgg
     3961 gcagagtata ctatgctatc gcagccaagg atgccaaaaa catcgttttc agcgttaagg
     4021 aagatactcc gaagttccct agtatgatta aatattattt gtttttggca aatacccatc
     4081 cctattacgg caagtataat ccgggccaga agatgatgta tacgggctgg ctatttatgg
     4141 ctttgattca gatcatcacc ggattcattc tttacaaacc acatactttt gcctggttgg
     4201 gcggggctct gggcggactg attgccatca gagtgatcca ctatatagtg acctggctct
     4261 ttgttttatc agtcctggcc cacgtttacc ttgatgtttc cgaaggaata ccggtgctca
     4321 aatccatgtt tacaggcaag atacctgccg attttgatca cggtgtccat gaagagcatg
     4381 aggctgaagt tggcaatggg attagtgctt agctattgga ggatgaaatt tgcataagct
     4441 gatggtagtt ggaataggaa attatattct gcaggatgaa ggagtggggg tgcacgccat
     4501 caaccggtta atgaaaatgg atctgccgag tgatgtggaa ttgcttgacg gtggtactca
     4561 cagttatgat ttggtggact tcttttgtca ggcggaaaag ttgatcatta ttgatgcgat
     4621 tcaggccgga ggagagccgg gaacgatgta tcgcgctccc ttggaggaaa tgggtttgca
     4681 gccacaggaa aactgtacgt ctttacatca gatgcatttc atcgaggcgg ttaaaatggt
     4741 caatttattg ggacactatc cgcaaatcat tgtctatggg attgagccgg aagtggttga
     4801 ttggggactg gagctgactc cccgggtagc cgagaaactg ccacgcctga ctgaaatgat
     4861 agcccaggaa atcagcgatt taatggcgca ctgattgcca gggccaaaga gaatatcgaa
     4921 ccagatgaat caaaccaaca gacgcgggta acgcagatgc tgaagaacac gttatgatat
     4981 aaaagatctt caggttgcgt gcggcaaagc cgcccgcgtc tgttagtaat ctttaagcag
     5041 gcaacaatta tgaatgtaaa aatgaacagt tatcggataa aaattaacgg gatcgtacag
     5101 ggagtgggct tcaggcctca cgtctatcga ctggctatgc ggtacgaatt aaaaggttgg
     5161 gttctgaact cgtccgcagg cgtcatcatt gaaattgaag gagaagaaaa catcatccgg
     5221 caattcttgc atcaagtggt ggaagaacct ccggcactgg cagtaattcg cgattggcaa
     5281 atcgacgcaa caccatggca tggatatcag gaatttacaa tcaaaatcag tgatgaccgt
     5341 gaagacaaaa cagtcatgat ctctccggac atcgccgtct gcaaggagtg ccgggaggag
     5401 gtcatggaca ggcaggatcg gaggtttgac tatcctttta ccaattgtac caattgcggt
     5461 ccccgcttta cgataataaa aggtctgccg tatgaccggg ctatgacgac gatggatgcg
     5521 tttcccatgt gtccggcgtg ccaggctgaa tatgaaaatc ctttgcacag gcgttttcat
     5581 gcccaaccca atgcctgtcc ggtatgcgga ccgcatacca ccctctgtga taaccgaggc
     5641 aaaagcgtta atggtgatgc cagggattac ttgaaacagg gacatattgt agccgtcaaa
     5701 ggactgggcg gttttcatct ggcggttgat gcttgcaacc gtgatgcggt agctaatctg
     5761 cgcaagcgca aaaagagaga tgccaaacct tttgcagtaa tggcgaggga tctggaggca
     5821 gcgcataagt attgtcaaat caatgctgaa gaagaaaaat ggctggcatc tccccaggct
     5881 ccgatcgtta ttttggagag aaatgaacag tgtttgctgg cggccgatat aattcatccg
     5941 ggaatcaaga cgctgggggt catgctgccg tatacaccgc tgcattttct gctgtttgat
     6001 gaggaattgg aaatactcat catgaccagt gccaatatca gtgatgaacc gctcattata
     6061 gataaccatg aagctttgga taaattaaag ggtatagccg attgttttct gttgcataac
     6121 cgggatattt acaacccctg tgatgattca gtgatgaggg tcacaagttt taatacgccc
     6181 catctgttta ggcgagcccg gggttttgtt ccccgcggta tcccgttgaa cattaaaaca
     6241 gaaccggttt tggctttggg cggagagatg aagaatgcct tttgcatcac tcgtcagggg
     6301 gaagcttttc tcagtcagca ttggggagat ttaaaccatt atctcaacta tgtcaatttt
     6361 cagcggggaa tcgaacgctt taagcaaagc ttgtatgttg aacctaaaat ccttgcccat
     6421 gatttacatc cggagtatca gaccacccgc tgggccagac aacaaccata tccaaaaaaa
     6481 atcgcgattc agcatcactt tgcccatatg gcttcagtca tggcggaaca tcagctgcag
     6541 ggtgaagtac tggggttgat ctgcgacggt accgggtggg gcaccgacgg tgcggtctgg
     6601 ggggggagat tctgcaagga gactgtcgcc atttcaagcg ggtagcccat ctccagtatg
     6661 tcccttatcc gggcggagat atcaataccc aaaagcccta tcgaatgagc ttgatatatt
     6721 tatccgcggc gctgggagaa aaagggcttg cccatgccga aaagattctg ccggatttaa
     6781 gcggacagga actcgctttc atgctgcagc aaatgaaaac gaatcactct gtcagccaga
     6841 cctcttcctg tggacggctg tttgatgcgg tggcagcagt tttgggggtt tgcggaatca
     6901 atcgctatga aggccaggcg gctgcagaac tggaagcctt ggcggatcaa agcgagaaag
     6961 ggcgttacgg gtttacattg tgcaaagagc aaaatatctg gttgatggat gtgctgccca
     7021 tgtggccaga gttgatagca gatttgcagc aaggccgccg gcgggcaagc atggcgcaaa
     7081 aatttcacct gaccctggtc gagatgtttg tcacgacgct cctccgatta cgtgacgaaa
     7141 gcggcctcac ccgggtggtg ttgagtggag gggtatttca taaccagatc ctgctgtatc
     7201 atctggttga acaattgaca gaaaaggaat tcaaggtctt ttatccccgc caggtcccgg
     7261 ccggtgacgg gggacttgca ttgggacagg caatcatagc aagtgaggtg ttggcataat
     7321 gtgtttgggt gtacctgcca gagtcgttga gaaaacaacc aactattcag cccgggtgga
     7381 tgtcaatggc aatcatacgg aaatcaatat tcagttgact ccggacgttg aaccaggaca
     7441 atttgtactg gtccatgccg gttttgccat ggaaatcatt gatgagagca tcgctttgga
     7501 aaccatgcac tatctggagg agatgctgca atatggacaa tgatgttaaa gaatacaagc
     7561 agcatttgtt gcgggcaatt caattattca gcggttctgt atctttaatg gaggtttgcg
     7621 ggactcatac catggccatt gccaagagcg gcatccgggg actgttgccg cctaatataa
     7681 aactcctctc cgggccggga tgcccggtgt gtgtgacgtc gcagggagat attgatgcgg
     7741 ttattgtaat ggttcagcac aaggatatta ttctgattac cttcggtgac atgatgagag
     7801 ttcccggaac acacagcagc ctgcaggaag aacgaagccg gggcgcggat atcagaattg
     7861 cctattcgcc gctggatgcg ctgcgggtag cgcgggacaa cccggacaaa gaagtagttt
     7921 ttctggggat cgggtttgaa acaacaaccc caaccattgc cattaccatt gaacaggcaa
     7981 gaagggaagg attgaataat ttttcggttt tttctctgca taaggtggtg ccgcctgcgt
     8041 tggaattaat attttcagat tctgaaatca aggtagatgg tttgctctgt cccggccatg
     8101 tttcagcggt aattggggtc aaaacttttg gaagtttggc cagaaaatac cataaacctt
     8161 gtgttgttac aggctttgag acgctggata ttctggaagg gatctatatg cttctgcagc
     8221 aattaaaaaa agaggaagca cgagtggaaa accagtatag ccgggtggtc aaaccggaag
     8281 gtaatccgat cgcctgtcag agcatggaac gtgtatttca gccggtcccg gctcgctggc
     8341 gagggttggg gatgattccc gccagcggct tgcagttgcg ggaggaattt agtgatatgg
     8401 acgcccgcaa gagatttgat attccggaaa ttgaggaaat tcccatcaaa ggctgcgcct
     8461 gcggcgaagt tctggccggt aaaatcactc ccctcgagtg tgttttattt ggtcaggcct
     8521 gtacgccctt gaatccggtt ggaccttgta tggtctcaca cgaagggtcg tgtgcggctt
     8581 attatagata tatcccaacg aaaggaagaa gttagatgac ccaggagaga atactattgg
     8641 cgcatggcag tggcggactg atgagtcagc gcctgatcga tgaggtgttt aaacaagcct
     8701 ggcgcaatga aatcatggaa gccatgctgg acggagcaat tctgcaagtc cctgccggtc
     8761 gtctggccat gtcgaccgat tcatttgtca tcaccccgct ttttttcccg ggaggagata
     8821 tcggcaaact ttccatttgt gggacggtca atgatttgct ggcctgcggg gcgactccgc
     8881 tttacctgtc aaccgccttt attatcgaag agggcctgcc cctggcagat ctccgcgtga
     8941 ttgcagcctc catggcccaa actgcgcggg aagttggtgt caagctggtg accggtgata
     9001 ccaaggtggt ggaaaaaggc agtgcggatg gggtctttat caatacgacc ggaatcggga
     9061 tcataccggc agacattgat tatcgtcccc aacgaattca accgcaagat caggtcattg
     9121 tcactggttg tgtaggcgat cacggtctgg caatcctggc ccggagagaa ggcttggcct
     9181 ttagcactcc agtcgtgagt gactgtgccg cccttattca gttaagagag ataatatccc
     9241 aatatggcaa tgcgataaga tgcatgcgtg atcccacccg gggaggactg gctaccgtgc
     9301 tgaatgaaat tgccgttcaa gctggcgtcg gcattcatgt caaggaagct gatattccgg
     9361 tggcgcccgc tgttcaggga gcctgcgacc tgctgggcat ggatcctctt tatatggcca
     9421 atgaaggcaa aatggtcata tttgtggctg ctgctttagc gcagtcatta ttgaatgatt
     9481 tgcgccggct tcctcaaact caagctgcgt ccatcatcgg ccagatcagt gctgaaacac
     9541 caggcatggt gctcattgag acggaattgg gagcccagcg tattctgggc attttagaag
     9601 gcgagcatgt gccccgcatt tgctgaatta ttttacagtc atttaaaaag acgtgtatac
     9661 tgatagaaaa agtaacgagg ttgcagtatg cacattagaa atatttttca acgtttgctg
     9721 attcccgttt ttattacttt atttgctctt tcggctttcc ctcctctggt tcaggcagcc
     9781 agcagcgagg gaggaccaac cgcttatatc atcctgattg acaagctttc catcaacgac
     9841 atcgatccgg tggctacgcc ggcaatgtac cgcctcacac agcagggcgc agtcggattg
     9901 gccagcaacc gaaccttgcg gggacacaat accatcgact gcagtttgac cctgggggct
     9961 ggcaatctgg ctcgcggtta tctcaacggt ttaatgggtt tcaatcaaaa tgaaatcgtg
    10021 ccggagcgca acaagacagc cgggcagtta tatcagaacc tcaccggcat tagcggagcg
    10081 gacagcgcat tgctgatggt aaactacccg gaaatattgg aaggcatgat ccaggagagc
    10141 gtcagcacca tacccggggc aatgggtgaa gttttgcgtg ccaaccagct caaagtctgt
    10201 gttttgggaa atggtgacat cggcaatgaa aggatacggc ccggcatcgc agtggggatg
    10261 gatgcccgtg gacaggtgcc gctgggagag gtgggttcag ctgtcagcag tttgtcgcct
    10321 gccagttttc tcagccatga gaccaattac gaattcattt ccgaacagct ggaacaaaat
    10381 cgctcccagg ctgatttgat tattatcgat ctctcagatc tggcccgttt ccaggaaggc
    10441 gatacagctc tccccgacat ttatacattc caaaaaaagt cgatcatgcg gaaaatggat
    10501 tatatcgtgg accaaataat gcagcagctc gatccccaaa aagatctgtt gttgttgtcc
    10561 gggatatctc catctcgaga agcaattaaa ttgaaggata attttactcc ggtgctggca
    10621 tatggcaaag gcttttccgc tggtattctg acttcttcca ccagccggcg tgactatatt
    10681 gtggccaata ccgaccttgc ccccacggtt ctgaagtttt ttgagctaaa agatgagcag
    10741 cgggtaatga ttggacaggt catgttttcc aaaccggaag cagagggtga ccgtcttgct
    10801 gcagccatcg atctggcggg gtcaaccagc actgccaacc gtctgcgctc tcctgttata
    10861 aaaagttatg tggttcttca aatcattgtt attctactgg cgctgctggc cattttccca
    10921 ctcaagcagg gcaaaccaat catcaagtcg ctgattgtct ccatggttgc cattcctctt
    10981 gttttgctgc cgctcaataa aatacctctg catgatgact ggctttatgt tgctgctgcg
    11041 attctcttga ctgcgggagt caccaaactg gcgttatact tctgtaaaga caatgctttt
    11101 aaaagtctga tgatgatcag tcttgttacc gtactgatga ttaatttgga cattatcatg
    11161 ggggctccgc tgataaaaag ctccctgctc ggttatgatc ccatgaccgg agccaggtat
    11221 tacggaattg gcaacgaata catggggatc ttgatcggca gcagcattgc agttggcacg
    11281 gccctttatg agaaatacaa acatcgcttg ctcctgttca tcattgcagt gttttttacc
    11341 agccaatgtt acctgatcgc cggacccaca ctgggagcca actctgatgg actgctgacg
    11401 gcgccggtcg ctttcctggt cacatttctt ctattacggc aggtgaaaat cagtttccgg
    11461 gcggtggttg gtatttttgg agcagtattg atggtggctg ccgggggaac aatgtatgac
    11521 atgcagcgac cggcggaact gcagacccat attggtcggg cggccaacca aattgctgcc
    11581 ggcggctggc aggagatcct gctgattata aaacgcaaag tcggcatgaa tatcaagctc
    11641 attcgctata ccatctggag ttatgttttt atggtcattc tattagtatt gtcactgttg
    11701 ttggttcacc cgattggagc catgcggcag ctgcgtcagc aatatcctgc tgtatttaac
    11761 gggtttatcg gcatcatcac cgctgcctta gtagctttgc tggtcaacga ttccggcatc
    11821 gtagccgctt caaccaccag catttatctt gtatttccca tattattact aatcatggat
    11881 atagatcagg acattcctga tacaagggca gaagagaatt aaacaaatgc tgccgtaaac
    11941 cggcagcatt tgtttgggga tatatatcgt aaaggattga atggaagagc cataattcgt
    12001 tattacggat tctttctttt caaactgtat tgggtgtgca gcaattcatg agatctgtgc
    12061 cccagggttt cctgcaggaa gtctgtatag agcatctgga tctcgtggtt atcatgggat
    12121 ttgcgcaaag ttttgctgcg gtcttcgaca tagatggctt ccatccgttg ctctctttgc
    12181 acgttgctgc gggtgatggg ttgaccgccg ccgccgatac atccgcccgg gcaggccata
    12241 atttcaatga aatgataagg agatgtgcct gcggccacct cgtccatgag cgcccgggca
    12301 ttgcccagcg tatgcgctgc cgccaccttc acgatggttc cgtccaaatc tatcgtggcc
    12361 tcttttaaac tgtccatgcc ccttacatca gtgaaatcaa gttcttccag tacccgaccg
    12421 gttacttttt cataaacggt ccgcagggct gcctccatga ctccgccgct ggctccgaac
    12481 aatactgcag aacctgtata ttcacccatc caagggtcaa atgcagtttc cttcagtttc
    12541 agcggattaa tccctgtcat ctgcagcaga cggacgatct cccgggtggt tagcaccata
    12601 tccacgtcgg aataatcact gtcggtcatc tccggacgca cggcttcata ttttttggcc
    12661 gtacagggca tgatggaaac ggaaaaaatt ttgtcaggat ggatcgacat tttttcagcc
    12721 caataggttt ttaccaaggc accaaacatt tgctgggggg acttgcacgt ggataaatga
    12781 ttcaaataat ccggataaaa aacctccgca aaacgaaccc aggctggaca gcaagaggta
    12841 aacatcggta acacgccacc attgcggatt ctctcgagca attcctgact ttcttccatg
    12901 atggtcaggt cagcggcaaa gttggtatta agaatgtgat caaaccccaa cattttcagc
    12961 gcacttacca attgccccat ttgcagctga tcgcggtcag ccccgaaagc ttctgcaatt
    13021 gccagccgca cggcgggcgc gatctgcgtc acaacgattt tttccggatc ggcggccact
    13081 gtcatgaatt catcaatctg ttcccggccg gaaatggcac ccaccgggca ggcgtgaata
    13141 cactgaccgc acatcacgca aggactctca aacaattcct ggccgatgga aggaaccact
    13201 tgggcatcaa aaccacggtg ggccaatccc aaggcattca cggtctgctt ggaggtgcag
    13261 aattcgatac aacgaccgca gagaatgcac ttgttgggat ccctgacgat cgagggactg
    13321 gaatagtcca gcaggagatc agcttcgacc tgggggaaac gattgtatct gacatcatac
    13381 tccgaagcaa tttcctgcaa ttcgcaactg ccgctgcgca cgcagtgcaa acagtcctgg
    13441 ggatgatgag ccagaattaa ttccaggatg gtacgacgag cttccagcac cctgggagtt
    13501 gcagttttga tcgtcatgcc ttcattcacc ttggtggcac aggctggcat caggtttttc
    13561 tgtccctcca cttcgaccac gcatatcctg cagttggcct tgacttcctg atcgggatgg
    13621 aagcaaagag tggggatctt gatgcctgcc ttttgagccg tatgcaatat ggaggtacct
    13681 tccggtacac tgattgggat gccattgatc atgacagttg tttcagacat ttctctcacc
    13741 gtcctctttt cttcctaacc cttggcgatg gcgccaaatt tgcatttcac catacaagcc
    13801 tgacatttga tgcacttgtc ggcatcgatg acgtgcggtt ttttgcgctc gccgctgatc
    13861 gcccccaccg gacaaacatc agcacaaagg gtacagcctt tacacttatc tgcatcaatt
    13921 ttaatatcga gtaattctac acacacgccg gcaggacatt ttttatcata aatatgcgct
    13981 tcgtattcgg aacggaagta gcgcagggtt gccaataccg ggttgggtgc tgactgcccc
    14041 agaccgcaca gggaagtgga ttgtacgacc cggcataaac tctccagagt atccagatca
    14101 gtcggggtgc ccttgccgtc acagattttc tccaaaatct gcagcatgcg catggtgcct
    14161 tcacggcaag gggcgcattt gccgcacgat tcttcctgag taaaatgaag aaaatatctg
    14221 gctacatcaa ccatgcaggt atcttcatcc atgaccacca gtccaccaga acccatcatc
    14281 gcgcccagtt gcagaaggga atcataatcg acgggggtat ccagcaaact ttccgggata
    14341 caaccgcccg aaggtccgcc gatctggacc gctttatagc gtttgccgtt ctggatgcca
    14401 cctccgactt taaatacaat gtcgcgcagg gaaatgccga tgggaacttc ggccagacca
    14461 gtgcgattaa tcttaccggt caatgcaaat acctttgaac ctttgctgtt ttccgtgccc
    14521 atgctggcga accaagctgc tccctgacgg atgataccgg ggacgttggc ataggtttcc
    14581 acattattga tattggtggg cttgccccat aaccctgact gggccggata ggggggcctt
    14641 acccggggca tgccgcgttt gccttcgatg gaatgcatca gcgcggtctc ctcaccgcaa
    14701 acaaatgctc cggctccggc agcgatttga attttaaagc taaatccgct gcccaggata
    14761 ttgttgccca agagtcccat ggattcagca tcccgaatgg ccttttttaa tcttttgata
    14821 gccagcggat attcagcccg aacatagata tagccttcat cagcaccgat ggcataaccg
    14881 cagatggcca tcccttccag cagggaatga ggatcgcctt ccagtacgct gcggtccatg
    14941 aaagctccgg gatcaccttc gtctgcatta cagactacgt attttttact gccgggagag
    15001 tggtaggatg cctcccattt gcgtcctgcc ggaaaaccgc cgccgcctct gccccttaat
    15061 ccggatttac tgatttcctg gattacttca aagggggtca gggacgacag tactttttcc
    15121 agcgcttcat agccgctgaa tgtgatgtac tgaacaatct gatccggatc gatgatgccg
    15181 caccgtccca gtacattctt gacttgtccc tggtaaaaag gggtctcctc cagcttgaca
    15241 atgttggcgg cggcttcata tcctctggaa tcctgggcga taatgtattc tggggagggc
    15301 tttccctcgg ccagatgttt ctgcaggatg gcaactgccg cttcgggttt tacgttgccg
    15361 tagataaaac gaggctgacc cggcatatgg acttccatca gcggttcggc atagcacatg
    15421 ccgatacatc cggtcgacat catttcgacc tcggcaccgt gggtttcgat gtaacgactg
    15481 accgcttccc agattttttc cccgccggcg gcaatcccac aagtacccag tccgattaca
    15541 accttgatat gatgctggga ttcttcgtat gcacttcgaa attgatgacg catttcattt
    15601 aagctatgca tttcacttcg ccatcctttt cctgatagcg cgccaagatt tccggaacgt
    15661 catcaggact tactctgcca taagcgtctt cattaatcat aatgaccggg gccattccac
    15721 aggcgcccag gcaggccaca ggctcgaccg taaacaacag atcatcagta gtcatctttc
    15781 cggcagacag gcccaatgat ttgttgattt tctccagaat cttgccgcta ccctgcacat
    15841 ggcaggcggt gccggtacaa acgcgaacaa tattcttgcc ccgtacttgc agatgaaatt
    15901 gaccgtagaa ggttgccact ccatatactt cgctcgaagg gatccgtaat gtttcggcga
    15961 tggttttgat cgcatcttcc ggaatgtatc cgaaaatatc ctgggtctcc tgcagaacgg
    16021 gaataatctt gccgttggaa tcacggtatt tatcaagcat tttatcaagc gccacataac
    16081 gcatatcctg aacatgttca ctgcaatcac acatactttg tcccttcttt cacaatcgaa
    16141 tttgcatttc tttaaatgca atcggggatt taaaatattg acagctgttg caataaatcc
    16201 tcgacgatcc ggtcctatgc ctcgagaact tcgattaatt cgtcaaaggc actggtgatt
    16261 tcatctaccg ggaataattt ttccatcacc gtgtaaacat cggttatttg ttgtttcact
    16321 tcagccgtca tttcttcggt actgatttgt ttggtcaaca aacgggagga actttcatcc
    16381 aaagactgga gaaaacggct catgatccgc ttgaagaaag gctgaatgtc tttggccatt
    16441 tcttttgata ccgggtcttc cataatgggg ccgcccagtt catagaattt gttcaattgc
    16501 acattcaatc tgaatgcagc cagtttggca attttgctct taatgaccag ctgttgtgca
    16561 gcctgatcgg ttaaataggt cttgtcgttc agcaactctg cgatttccaa atacttggcc
    16621 cggatacagt attgcagact gactcccaca ttgtatacct catgggctga ccgggggcag
    16681 ggaagagaac tggccatctc ataagctgct ttggtgtggt agttgatttt aattgctttt
    16741 gctgttccca ttgctcatca tcctttacat tagttttata tgtgaaataa ataacaatat
    16801 cttgaccaac tattaatgca agttgtatgc caaacaaaaa cacattgttt gtagcctttt
    16861 cgcaaaaagc tgatgatgga acgtaaaaat cggctgttgc aggcttgatg ttacagtgaa
    16921 atccttagcc ggctttaatc gaaaaagcaa atacatcaga ctggggaaat aaaaaagtgt
    16981 aacatggatg taactataat gtatgatcag tatgatgcgg attacaaaaa tgtgacaaaa
    17041 ttgtcgcagt tacaggcgaa aacaacgaca gattgacaaa ataatagttt ttggcctttt
    17101 gccttggaat taccgggaat atttttaaaa taacgaaaga tcttgctgct aatgatataa
    17161 taattatttg aaacagctca ttcggcagag tcgggtaaac ataagcaaaa aggagatgga
    17221 accattgcgc tttatgcagg aatacaagaa caagttagtc actgcaaaaa aagcagttaa
    17281 aacagtcaag tcaggggata gaatacaatt caattcttat aatggcaatc cccccgtact
    17341 ggatagggcg ctggccgccc ggcgggatga attggaagat gtcgttctgt ataccagcgt
    17401 aaccatgtat ccgctgcaga ccattgcctg cgatccggaa ggccggcatt ttatctacaa
    17461 caatatgcac gcttccgggc atgatcgcaa gttggccctg gcgggcaact actattatgc
    17521 gccggttacc tattatgaat tgccgactat tttaaaaaac gaacgccggg atgtggtcat
    17581 gttacaggtt ggcagcatgg atgacatggg ctatatgaac catggaccca acgccggaca
    17641 tgcccaggat ttggtcagaa acgccaatct ggtcatcgtg gaagtcaatc gcaatatgcc
    17701 cactgcttat gggggacatg atgcggcggt acatatcagt gaagtggatt tagtcgtgga
    17761 gggagatcat cagccgttgt ttaccttgcc ggcgttgcag cccaatgaag tagaaaagca
    17821 aattgcccgc tgggttatga ctgaaatcga ggacggggcc tgcattcaac tggggattgg
    17881 agccatgccc aactttatcg gggaattaat cgtcgaatca gacctgaaaa atctgggttg
    17941 ccatacagaa atgttggcgg atgcatattt gaagatatat gcatccggta agatgaacgg
    18001 aagaaacaaa ggcatcgaca aaggcaagat ggtgtatgga ttcgccatgg gctcccaaga
    18061 tttatatgac tttatcgatc aaaacccggt ttgtgcgact tactccatcg ccgatgtcaa
    18121 cacgccgcat gtcgttgccc gtaatcccaa aatggtagcc atcaatggag ccatcgaagt
    18181 ggacttgttt tcccagatca attcggagtc ttccggtacg cgtcagattt ccgggaccgg
    18241 cggtcagctg gattttatga tgggcgcatt catgtcggaa ggtggcaaag ggtttatttg
    18301 tttgtcgtcc acctatacca atcaggaggg caaactctgt tcccgcattc gaccgacact
    18361 tagtcccggc acgattgtaa ccacgccccg ttccttggcc cattatatcg taacggaata
    18421 tggcattgct tatgtcaaag gcaaatctac ctgggagcgg gcggaggcgc tgataaaaat
    18481 tgcccatccg cgttttcagg atgagttgat caaagaagcc caagagatga acatctggcg
    18541 tagaagcaac agggttaact aaaatacata agttaaataa acttcattgc gaaacgtctt
    18601 cttgaaatga aaacggatcc gtttttcttt tttctgccgg ttggggacct acttcgataa
    18661 tcgccatggt gcagcgggaa atgcagatca gggtttcttc ttcatcatag atgcggatat
    18721 cccaaatgat ggtgcgccgt cccacatgga taggggtgcc gaccgcatga actattccgc
    18781 tggctttgct gcggatatga ttcccattga tctccagccc taccgctcgc tgccaactgg
    18841 cgtcaataaa ctggtagctg ccggcagaag ctactgtttc ggcaatgacc agggaaacgc
    18901 ccccgtgtaa cagtccaaag ggctgatgaa ctttgggcgt aaccggcatt cgggcaacga
    18961 ctctctccgg cgtatactca aagatttcaa ttcccagtgt ccccatcacc gtatggtcta
    19021 tatcaaaacc ggaaacatcc tgcatggcga tcactccttt gttagattca ggctaataaa
    19081 cttatgataa cacataagtg ctcaggaaaa attggatgaa tgatagcatc aaaaacgttg
    19141 gtctgaatat ttctgcacgg acaaaccaaa atataatatt agaataataa aaaatatttt
    19201 taattgtaat attattacgt aaaattacat tgcctgatta attatgctta attataatag
    19261 taattaatga cttatagtga tggttcatat tggttgcctg aaaaagttct gttgaagttt
    19321 tttagagagg caaggcgcag ttttgtaaac tgtgtcatga atatcaacaa tcgggaaggg
    19381 ggagcagtgt tctcggtatt ataaaatacc ctaataataa ttaaggaggc atagaacgga
    19441 tggcacatct ttataccagc gacgagcaga ttgaccgact tgtggatttt aagggtgctc
    19501 gtgaatacca tcttacgaag gatcgccgta aaaaagacaa acgtatgcca ttgaaggagg
    19561 ccatcgctga atttgttcat gacggtgata tttttgctga aaccggtttt tcctatgtca
    19621 ggagtcccat tcaggcttat tttgaaattg ttcgccaaca aaagaagaac cttaccggga
    19681 tcggttctcc catgtccaac tgttcctatt tcatggcccg gggatgtatg aaagcaatcc
    19741 atttgtctta cattggcgtg gaaatgcgcg gcaatgacaa aaactttgcc cggatcatca
    19801 agaacaagga agccgagatt ctttccttct ggagccatgg gaccatggcc cagggattca
    19861 aagccgccca actgggtgct ccctttattg cttccaagca attgctgggt accgatatga
    19921 tcaagcaaaa tccctatgta aaagttattg acaacccggt cggcatagat catgatccgg
    19981 tttgcctaat accggccttg ttcccggatg tctgcattat ccatgtccaa caggcagatc
    20041 gattcggcaa tgcggtgata agcggcccgg ccatcaatga catagcgctg gcagcggcct
    20101 gccgcagagt cataattact gccgaaagaa tcgttccgga aatgaccatc cgggataacg
    20161 gcaatgcaag tattcccttt atgtatgtgg atgcggtggt cgagcttcct tatggagcct
    20221 atccgggatg ctgcccagga cattatttct ggaatcggga gtggtgggaa ttcctgatcc
    20281 gtattggcac ggtaacctat gacaatatga ttgattttac cgatcgctgg atcatgaata
    20341 ccaaagacca atatgatttt atagataaag ccggcggaat ggcggttatt gacaaggctc
    20401 gccgcttgat ggaagcagcc cagggaaccg cagatgacac tgcaatcagt tttgactatc
    20461 aagaagtaat acccaaatgg gactaagcaa ggaggggaat tatgagcaat gtagcacccg
    20521 ctaatccatt ggagatgctg gcctataatc tgtctatgat gatcgaggac aaccagattg
    20581 tatatgtggg aaccgggctt cccatggtgg gaggaatgtt ggccaagaag acgcatgcca
    20641 agaacatcac tttggttttt gaaagcggtg gacaggatcc gctggaaggc tgtgatgtcc
    20701 catggtcagt cggttgtccc tttacctacc gtaaatcacc tttgactatg gagatgtcca
    20761 tctcctttgg ccagtgtgcc ttgggatatg ttgatatcgc ttttcagggt ggcgctcagt
    20821 ttgatatgta cggtaatgtt aacacttccg tgatcggcag ttacgaaaac ctaaaatcca
    20881 ttctgcccgg cagcggcggt ggcaatgata tcggttcact gacggagaaa tcagtgctgg
    20941 ttggcttgca gctgcccgag cggtttcctg aacgagtgga ttttctgacc acaccgggct
    21001 atctcgaggg tggagacagt cgagaaaaag ccgggcttta tggtgggccg atcgcggtcg
    21061 taacccaggt gggcatctat gattttgaac cggaaagcaa acgcatgcgg atcaaagccc
    21121 tgaacccggg tatgacagta gagatcgcgc aaatgtgcac cggctttgaa ctgctggtac
    21181 cggaggaaat ccatatggtc gccccgccgc cagacgaagt gctggaaata ttgcggacgg
    21241 tggtcgatcc ccgcggcgta tttaccaaga tgccggggat gtaaaaatct gtcgtgaaca
    21301 aacagcaaaa gggctggcat ttgccagccc ttttaatttg gctttattga acgaatggtt
    21361 cccgcttatt taacccgggg cgggaattca ataaagatct tgtgggggtc aacttcttga
    21421 cgtaatactt ctaaaatatc agacggcggt gctggaattt cctcaacttt ttccggtacg
    21481 agcaattcaa agccgctgca ctgctgggca atgtcaaccg tacaataagg attcagccct
    21541 ttaacgcgca tacgtttgct ttccggttca aaatccatga ccccgatttg ggttaccaca
    21601 gactgcggac ctttgcccag caaacctgct tctatacggc tatttccgcc gctcaggaac
    21661 cccggactgg tcatgaagtc caatttagca gggaatttac tgggatcctg caaccctacc
    21721 aggacaaatg tctctgacag ggaactcagg tcgttgccgc cgccgctgcc ggtcagacgg
    21781 gacttgaagt tgtggaaatc tccgccgatc atgtgagtat tcagattgcc gtacatatca
    21841 atctgggctg caccctggaa agcgatgtca acaaaaccgt tggcagccat ggcaaaggag
    21901 tgtgccattt ccatgctcat cggcgcccgg cggaaggttg ccgcgccgcc tacggaccag
    21961 ggcatgggac cttcccaagg atcttgtccg ccactttcat aaaccaaggt gatatgaggg
    22021 gcatgggttt ttttggctaa catggcgcct accatgggga gccctgtgcc gacgtaaacg
    22081 atcatgccgt catgtatttg cagagcaatg ttgtaggcca gttgttcaaa aggattagct
    22141 ggtgctggtg tattcaaaag gttcgcctcc tttattattt atcttactcc catttgggga
    22201 tgacggtttc ataggtaaaa tcgaccccga tgtcctcaat caagtattct tccgcctttt
    22261 ccaatctgcg caggttatca atatatgaaa atccacccaa atctttggcc agacgatcaa
    22321 ccaaggcata ctggtcgggg cagtcgtaaa tccaattttt catgaaatcg ctcatggcga
    22381 catcattttt ggtagcgata cgcatcagcc attcccacca ctctctggcc caataataat
    22441 gaccgggaca ggagccgggc agacagccat aaggcagttc gaccacagca tcgacaatca
    22501 taaaggggat gacggtatta ctgttgtcaa aacgaatact cagttcggga acgacttcct
    22561 cgcaggtaat aataactttt ctggaagcga atgccaaggc aatgtcattg acggccgggc
    22621 cgactatttt cccgtttccg aaaatatcag cctgctggca gtgaatgatg cagacatccg
    22681 gatgaagggc tggaatcaag accaccggct gaccgctcca gggatctttg acttccttga
    22741 cataagggtt atacttgact atgtcagtgc cgagcaattg atgggctgca agatacggag
    22801 agccgtattg ggcagcttta aatcccaaag ccatcgaacc atggctccat tcagagataa
    22861 tttgagtttt gccttccttg acccctttgg aaaactggcg gtcagtgcca cgcatttccg
    22921 cgccacagta agaattatgg gtccaattgc agcatcccgc aaatacagca aaggaatgca
    22981 gcgtattggg ggaaccgatg tattggagat tctttttatt ctgacgcaga atttcaaaat
    23041 gggcctggat cggtgtgcgg acataagaaa aaccggaatc agccataata catccatctt
    23101 ccacgtattt ggcgatggcg tccttaagac tcattctctt gtcttctttg agacgacttt
    23161 ttttcaggtt ggtttcccga aaggactcat ggt
//
LOCUS       DAOTHZ010000033        22699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2685_length_22699_cov_6.625066, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000033 DAOTHZ010000000
VERSION     DAOTHZ010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 22699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22699
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2685_length_22699_cov_6.625066"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..954)
                     /locus_tag="P5549_05485"
     CDS             complement(19..954)
                     /locus_tag="P5549_05485"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRW12557.1"
                     /translation="MELKKDLLGLRGMSREEMELILNTAVPMKDIINRDIKKVPTLRG
                     KALVTVFYENSTRTRTSFEIAGKYLSADTVNLAVSTSSVQKGESLRDTIKTIEVMGFD
                     LMVMRHGISGTPHYVARNTKMRVINAGDGANEHPTQALLDMFSIKEKKGTLENLKVAI
                     VGDILHSRVARSNIYGLSQFGCEIRVVGPATLMPPEVEKLGVKRYHSLDLALKDVDVV
                     NVLRIQRERQSAGLFPSLDEYSNLYMLSPERLQNAKEDVLVLHPGPMNRGVEISSSLA
                     DSNTAVINEQVTNGVAVRMALLYLMMGGTKDEIAN"
     gene            complement(1076..1627)
                     /gene="pyrR"
                     /locus_tag="P5549_05490"
     CDS             complement(1076..1627)
                     /gene="pyrR"
                     /locus_tag="P5549_05490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRW12558.1"
                     /translation="MRLETKKKLMDETAVKRTITRIAHEIIESNKGVEKVALIGIRRR
                     GGPLAQRLAEKIEEIEGIKVPVGILDITLYRDDLTTLASQPQVHRTEVFFDVNDLNVV
                     LVDDVLYTGRTVRAALDAIIDLGRPRCIQLAVLIDRGHRELPIKADFVGKNIPTSQKE
                     VIAVLVEEIDGSDEVVIQEIFYD"
     gene            complement(1787..2704)
                     /locus_tag="P5549_05495"
     CDS             complement(1787..2704)
                     /locus_tag="P5549_05495"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRW12559.1"
                     /translation="MPDFETLLVTEEMEGERLDSFVSDSLEDLSRSVVQNLIKSGQIR
                     VEGRASKASYRVKTGDTVQVEVPALQAVELVPQNIDLPIIYQDGDVVVVDKPKGLVVH
                     PAHGNWDGTMVNALLYHIKDLSGINGELRPGIVHRLDKDTSGVMVVAKNDRAHHSLAE
                     QIRVHSINREYIALVHGIIKENLGRIDAPIGRSTTDRKKMAVVKDGREAISDYEVLQR
                     YPNYTLVKVKLLTGRTHQIRVHFAYIKHAVVGDPLYSSARKHFNLDSQALHARLLGFV
                     HPRSGEYMEFTSPLPQYFQDILTQLARGE"
     gene            complement(2922..3746)
                     /locus_tag="P5549_05500"
     CDS             complement(2922..3746)
                     /locus_tag="P5549_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12560.1"
                     /translation="MNKKPWLKKVVTGALLGGLLLGSAGYVWAADSSSVSNTIDNVKT
                     KFQSMMPGGRGHGHGMMGGEMGMNLNLQTLVGEGVISSDESTAIQAKIDALQAERQAE
                     MDKIKAMTAEERQAYFEARKTAADDLEAVKKDLLTMLVDEKIISSDTADAIRNNQQAQ
                     RQEEMQRRTTDQLSALVADGTITSAQSDAILAAIQAEQAERQSEMEKVKAMTAEERQA
                     YFQANQPTRGNDLAVLVSAGALTQEQADAVQKALGGPRGGGQGHGGKGPGGMGFPQ"
     gene            complement(3899..5389)
                     /locus_tag="P5549_05505"
     CDS             complement(3899..5389)
                     /locus_tag="P5549_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW12561.1"
                     /translation="MIKSVQTRLFLLISLLVTCFVLLSLLVNSNYLEQYYMNHKKSQL
                     LQNARQIEAIYQGEPDDIILEMERLQSQSALSMIILDEAFVLKYPVYGQRPSLGGLPV
                     PRLDGRLEAGLDYIRNNRAVLEQHDVFALIEDDRLSIDFLNLFTRLDNGDYLVLATPL
                     SAIRESAAIANRFFFFSGLVTILIGLLAAFFFARRFTRPIRGLNQIAQKMSQLDFSEK
                     YPVTSQDEIGELGHSINSLSDQLGKAISELTRANRKLQADIEYERSLDEMRKEFVSSV
                     SHELKTPIALIQGYAEGLKLNVNEDEVSKDFYCDVITDEAHKMNLMVRDLLDLSQYES
                     GTFKIERRVFDMFDLLARVISKYQPIFTEKSVVPQLNWEQNEGELPVYADMMRIEQVL
                     INYLNNALNYMDERRQLAITLHIGEEQVRIAVYNSGQPIPNESLDKIFNSFYKVDKAR
                     TRAAGGSGLGLSIVRAILQQHHNDYGVLNCADGVEFWFEVDRYDEN"
     gene            complement(5386..6060)
                     /locus_tag="P5549_05510"
     CDS             complement(5386..6060)
                     /locus_tag="P5549_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW12562.1"
                     /translation="MTKKVLLADDEDRMRKLLSDFLKREGYAILEAANGKEALDIFQQ
                     TDDLSLLILDVMMPLMDGWEVCREVRKTSRIPIIMLTARDQEADELMGFELGADEYIS
                     KPFSPPILIARVNALLRRTEGKNYPPRTIGGLVIDENAHYVYVNGEVIDLTPKEFELL
                     VYLADHEGRALNRDQIVNAVWSYDFYGDMRTVDVHIKNLRLKLGEMGDCIQTVRGIGY
                     RFEGRK"
     gene            complement(6141..6587)
                     /gene="lspA"
                     /locus_tag="P5549_05515"
     CDS             complement(6141..6587)
                     /gene="lspA"
                     /locus_tag="P5549_05515"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRW12563.1"
                     /translation="MRFWLTAGFVLILDRFSKGWIMQNMNVGDGQAIMGDLVYLRYIH
                     NEGAAFGLMQGGGLLFVVIAAVVIAGAVYFLFKYHLPPMAQYALGLITGGALGNLIDR
                     VMYGSVVDFISVGWFPVFNLADCGIVCGGTLLVLWLYLLETGQKTE"
     gene            6894..8246
                     /locus_tag="P5549_05520"
     CDS             6894..8246
                     /locus_tag="P5549_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12564.1"
                     /translation="MNLQEMYKKKLMSAEEAVKLVKNHTEVWIGIAVPVPLALVNALV
                     DREPEVEDITINHIVDIHPHNTWHKLDRDTNIRVDCGYPSASRDKVVCGDFTISPIRF
                     HEMPKIYTNENFRPMHVAMLNVTPMDQHGYFCLGLGADTTVAIAQNAARKRRAGDDRY
                     TVLAQVNQQVPRSRGLNYIHISEVDAIVEQDQDLAVLPESGPLSPEENTIGHYCAEFV
                     KDGSTLQLGIGSIPNAVALAFLETHVRDLGIHSEMACDNMIDLWENEVVTNRRKTFMP
                     YRSIFTFAMGTKKLYDWIDDNPGVEFHPVNFVNNPHIIGKNDNMVSINACLQVDLTGQ
                     ICSESMGYKQYTHPGGQLDFVDGAFQSKGGVSIIATPAVAYPRSNNGQPVSKISPQIT
                     PGGIITTPRSCADYVVTEYGPAKIKGQSVRQRVKNIINVAHPDFRDELRFEARRRDLI
                     "
     gene            complement(8543..10699)
                     /locus_tag="P5549_05525"
     CDS             complement(8543..10699)
                     /locus_tag="P5549_05525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW12565.1"
                     /translation="MNRPKRQGTAHSAENFNKNQPARVRFRLYLVLVLLAGLLFIAYT
                     YLHYIHRSETEAINLAQAFEAFIGLEILDELEATPADIDKPAYSALKEELTTFVERNG
                     KMRFAYLFVYHDGRVYFLADSEPPESPAYSPPGQAYDGYSEQDFNRLRQGESIITPLV
                     KDQWGTWVTVTVPVINPHSGQTLAAFGIDYPAWQWYAEPLLNTGYAAALVIGLLLAFN
                     AYYRKTVSNELLRQERDKLAVMTRKLGESEELFRRVFEKAPIGIAIVSDFRFISVMNQ
                     QFEQILGRSFTELAALNWKEMTHPDDLPQDLALFAQFKAGQNDGYEMDKRFIKPDGKA
                     VWVKMIIAPLTFDEQGLVREYLCLLEDITERYRMIRELQESERSLLYTNAHDYLTGLY
                     NRRYFEAEKLRLDRAENLPLSVIIADINGLKLINDALGHEIGDQLIRETARVMAACCR
                     EGDMLARTGGDEFTILMPNTSREEAQALFKQIESAFRDEFRIQEENYMSVSLGFDTKE
                     SWSEDINQVIKSAEDIMYRRKLLEHKSSHGSIIAAMKSTMFNISHETEAHANRLLEMS
                     RRIGARLQLSNDEMGKLELLATLHDIGKLGIDRRVLNKETPLNEEEWKEMRKHPEIGF
                     RIAMSTHELMPIAEYILSHHERWDGSGYPQGLAGENIPLLSRILSVIDAYDAMTSDRV
                     YRHAVSREEALEELKQFAGTQFDPRIVEIFINASCG"
     gene            complement(10818..11606)
                     /gene="tsaA"
                     /locus_tag="P5549_05530"
     CDS             complement(10818..11606)
                     /gene="tsaA"
                     /locus_tag="P5549_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRW12566.1"
                     /translation="MENICLNPVGRVVSSVKTAKEMPAGGVPAVIEILPPYEAALLRI
                     EENSHLWILSWFHQSDRSNLVTPPGVNRDLPEYGVFALRAASRPNPVAMSLTKLIKRE
                     GHRLFLEKLDAVDGTPVIDIKPYFENDIVFSPVTSYIPGKNREYRQNQMLRRAIAHHQ
                     EECLHLQLAVRMALVAEDIFGHLNRPDLIVSVRGSACLADTIQGLSRARLANPPRFSF
                     VPSLTIQQTNWDLADKGTLTLTWLGPTAMLELEDEELLNIDFAN"
     gene            complement(11764..12039)
                     /locus_tag="P5549_05535"
     CDS             complement(11764..12039)
                     /locus_tag="P5549_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HRW12567.1"
                     /translation="MQEFYLNGAVMTDPQTTHRHLQQALSLPDYYGGNLDALWDCLVS
                     DFSEKTVYFTNLPAMLANLGAYGDALLELLWEAAASNSNLHLIIEDE"
     gene            complement(12250..12762)
                     /locus_tag="P5549_05540"
     CDS             complement(12250..12762)
                     /locus_tag="P5549_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HRW12568.1"
                     /translation="MQRKPIRSWAIMIALLLALACLSGCQAPVYEQPSAPAPQQTSQT
                     EAGQPSSETAAVTVQAGQRYSTPEAVAAYLHQYHELPPNYLTKAEAQQLGWNNAEGNL
                     WEVTEQMSIGGDRFGNREGFLPKASGRQYYECDINYRGGYRGAERIVYSNDGLIFYSA
                     DHYQTFQQLY"
     gene            complement(12926..14515)
                     /locus_tag="P5549_05545"
     CDS             complement(12926..14515)
                     /locus_tag="P5549_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW12569.1"
                     /translation="MDRLPVKHDQLLQELHNLRQRNTELEKNRTWLLQTIEQLKKTEG
                     ELAEKFINRTVELSKERQRLFAILDSLPFHVSLFAPDHKVIFANRAFRDQHGESNGQA
                     CYEYIFGLNQPCAYCETFAVFETGQPHRWHMTTPDGRQVEVYNIPFLDTDGADLVLET
                     SIDHTQHYQLMEELQKSEKRCQLLFHNSPEMISVISMQDGTYLDVNQRFLDCMGYTRA
                     EIINRTPMELGIWENDPLTNHSIRRELEEINQEHTAEYRIRKKSGQLITVSASSMALY
                     LDNELCRICMMRDISREKKMEAGLEKLDRLNIIGQMAASISHEIRNPMTSVRGFLQMM
                     RNYERYQEDGEYFDLMIEEIDRANGIISDYLGMARDKLLFLDQVSLDEIIASLTPMIR
                     SDANQREISIHLELGNVPLLHLYENEIRQLILNLARNGLEAMGSHGELAIGTCVEGRE
                     VVLYVRDQGTGIPEEVAEKLPTPFFTTKESGTGLGLSVCYSIAARHRAHIDYETGAQG
                     TAFFVRFPTDSAADSITAAEA"
     gene            14775..15320
                     /locus_tag="P5549_05550"
     CDS             14775..15320
                     /locus_tag="P5549_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12570.1"
                     /translation="MLNDIIITVLILLGGIAIYFALLGMIILYNRYIAAPSHRQPLMT
                     ERTRPEDVPPAEADLQSPTGEMQADPLPDYADFADRQTERHLPEPGRTGLKLPAAGLP
                     KCNIPKLNLPRPARTKKRTQPPEPVDPVRRELKKQEEAFRRRMQQRDEELLQELKQRR
                     EESRVAGQPDSSTEPVHPTGM"
     gene            15668..16963
                     /locus_tag="P5549_05555"
     CDS             15668..16963
                     /locus_tag="P5549_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12571.1"
                     /translation="MNYQEMYQRKVVSVEEALNQIQSGQEIACSLIGCEPVTMLSQLH
                     TIKDRVEDVSVLTALLMNEYEFFMNPDMKGHFLLNSWFYTGGARQAHPLGTVSYVPLE
                     LHRCISSRMAYRRPDIFLGCACPMDKHGYLSLSMDTVCEKELLENAAQVILEVNPNLP
                     RIGGDTLVHISQVDYILETTRALPTLPEVQLNDKEKQIGAYVAELIEDESTLQIGFGN
                     IPIAVTRCLTDKKDLGVHTEMFSEGLLDLYECGALTNRKKTLWRDKFVADCALGSQRL
                     YDFFDNNLAVEFHRGSVVNDPRVIGRNHKMVAINSALEIDLSGQCCAESIGSRHYSGT
                     GGHKEFGQGTQYSPGGKSIIAFLSTARSDTISHIVPQLQPGAVVTTSRIDVDYVVTEY
                     GIASLRGRSVRERITELINIAHPNFRDWLRSEAERNMMW"
     gene            complement(17084..17302)
                     /locus_tag="P5549_05560"
     CDS             complement(17084..17302)
                     /locus_tag="P5549_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12572.1"
                     /translation="MLNKNEVIARFLTCLVLFILTGTEVITGWPATLCGVIGTIELAT
                     SLLGYSPLMEARTVPQRERPTHAPRLAH"
     gene            complement(17575..18912)
                     /locus_tag="P5549_05565"
     CDS             complement(17575..18912)
                     /locus_tag="P5549_05565"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HRW12573.1"
                     /translation="MTQKLVIIGGVAAGMSAAAKARRIQRDMDIVVYTDDQYISYAGC
                     GLPYFIGGRVESEDKLVARTPQEFARQNIIVRTMMRAEEIKPEEGTVVLRDLQNHSAT
                     QDHYDALVIASGARALVPPLEGMDLEGVFALRNIHDSLQIKAYIENHKPHKAVVIGGG
                     YIGLEMAENLTERGCQVCVIERAPHIIPNMDEDMAQILTQYLEAKGVEVRTGEAVNGF
                     FGEGRVAGVITDQGELAADFVLLSIGVVPNSEIAAAAGIELGVKNAIRVNQKMETNLP
                     GIYAAGDCATTLHLLSGNEVYIPMGTTANKQGKVAGENAAGGNASFKGVLGTGIAKVL
                     EMEISRTGFSEHECRQAGIDYVTQRISSRTAAHFCPIARDIHLKLIMDRSNRRLIGAQ
                     IVGYTGAGMRIDMLAAAITVGATIEDLIDMDLAYSPPFSPVWDPVLVALNQFE"
     gene            complement(19019..19843)
                     /locus_tag="P5549_05570"
     CDS             complement(19019..19843)
                     /locus_tag="P5549_05570"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HRW12574.1"
                     /translation="MSLLFHATNFVKVNRHGDTVTAECTLLSNAVEAVAWVDASVQTL
                     QISRAGWANYRSSAGETGVFDLPALIGVEAYITGGPVLRKVLGGPGLELPYDLISECF
                     RGVLQAETYFFLERGFVSAKQYDDFCEQHSIGACRFYSHLEETELQWTEWLGSTERDY
                     NLFNRCKTVSITQQAVNRLVSASFMDSFHHLGVSLEIDPQGVVLSAQGNFINAPDPIC
                     FQNDAHLFKLPGLNLREMNKKEIAALTGGPEGCNHLVDLLYEIKKALQANEVRPQS"
     gene            complement(19885..20562)
                     /locus_tag="P5549_05575"
     CDS             complement(19885..20562)
                     /locus_tag="P5549_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRW12575.1"
                     /translation="MQEKQGTSDFFVVIACLFVSSLLLSNIIAGKLILIAGVVLPGAV
                     ILFPLAYIIGDVLTEVYGFQKTRRVIWTGFVCNLLMVGVFYLVMAIPSPAFFAGGEAF
                     ALVLGMAPRIVMASLLAYVAGEFLNAIVLSKMKLFTGGRFLWMRTIGSTLVGEGMDTV
                     LFITLSFLGTVPAAVLLQMMLFQYLFKVTYEMLATPLTYAVVGWLKRKEGLDTFDYGV
                     NYNPFKS"
     gene            complement(20672..21127)
                     /locus_tag="P5549_05580"
     CDS             complement(20672..21127)
                     /locus_tag="P5549_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HRW12576.1"
                     /translation="MGISDQKVAGPPDTLTTYALGSCVCICLYDRLRRIGGLAHILLP
                     EAFGDVSGKNVYKFADTAIEELVRGMIKQGCAHLHLTAKIAGGANMFVSEGKSIGDRN
                     IETVTQELQRLKIRIVAQDIGANYGRTAVFNTQDGSLTVKTLGRGNKVI"
     gene            complement(21139..>21446)
                     /locus_tag="P5549_05585"
     CDS             complement(21139..>21446)
                     /locus_tag="P5549_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRW12577.1"
                     /translation="DISEMDMSVIMEIGNVLAGSYVSAIATMTDLDIRLSTPQVAIDM
                     VGAILNYPAAYFGVMDDNLLFIEENFLSEENTIRSHLLIMPEPDSLSLIFESLGMSL"
     assembly_gap    21447..21546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21547..21911)
                     /locus_tag="P5549_05590"
     CDS             complement(<21547..21911)
                     /locus_tag="P5549_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HRW12578.1"
                     /translation="MGNYLNLNEYQLSALSEIGNIGAGNAAAALADFMADTIMMSVPE
                     LQIIDVNSMANILGGPENEMVGILVTMNCDVRGMLLFLLEKEFVCLLLNVLLSKDINE
                     FADISEMDMSVIMEIGNVLA"
BASE COUNT         5343 a         6190 c         5588 g         5478 t
ORIGIN      
        1 tcgatcactc tgccgccctt aattagcaat ttcatctttt gtgcctccca tcatcaggta
       61 taacaaagcc atgcgcaccg ccaccccgtt ggtcacctgt tcgttgatga ccgcggtgtt
      121 cgaatccgcc aatgaggatg atatttccac gccccgattc atcggtccgg gatgcagcac
      181 caaaacatcc tccttggcat tttgcagccg ttcggggctc agcatataga ggttggaata
      241 ctcgtccagg gaaggaaaca agccggctga ctgtctttcc cgctgaatgc gcagaacgtt
      301 gaccacatcc acatccttga gcgccagatc caggctgtgg taacgcttca ctcccagttt
      361 ttccacctcc ggcggcatca acgtcgccgg accgaccacc ctgatttcgc agccaaattg
      421 actgagtcca taaatgttgg aacgcgccac ccggctgtgc aaaatatcgc cgacgatggc
      481 tactttcaga ttttccagcg tccccttctt ttctttgatg ctgaacatgt ccagcagcgc
      541 ctgggtgggg tgctcgttgg caccgtcgcc ggcgttgatg acccgcattt tggtgttgcg
      601 ggccacgtaa tggggcgtgc cgctaatgcc gtgacgcatg accatgagat caaagcccat
      661 cacttcaatg gttttgatgg tgtcgcgcag gctttcgcct ttctgcacac tgctggtcga
      721 aaccgccaga ttgacggtat ccgcgctcag atatttgccg gcaatttcaa aagaagtccg
      781 ggtgcgggtg ctgttttcat aaaaaaccgt gaccagggcc ttgccccgca aggtgggcac
      841 ttttttaatg tcacggttga taatgtcctt catcggaacg gcggtattaa ggattaattc
      901 catctcttca cggctcatgc cgcgcaggcc caacaaatct tttttcagct ccaacgtttt
      961 tcaactcctt tgtgattggt gaataaaaaa gccttccctg ctttttcagc aaggaaggca
     1021 atatggttaa tcgctgctcc cttgccggtc tctcgtaccg aattaaaggg ttgggtcaat
     1081 cataaaatat ttcctggatg acgacctcgt cgcttccgtc aatctcctcc accagcacgg
     1141 caatgacttc tttttgtgag gtgggaatgt ttttgccgac gaagtcggct ttgatgggca
     1201 gttcccgatg tccccggtca atcagcaccg ccagctgaat acagcgcggc cgtcccagat
     1261 cgatgatcgc atccagcgcc gcccgcaccg tccggccggt atagagaacg tcatccacca
     1321 gcaccacgtt taaatcattg acgtcaaaaa atacttccgt tcgatgtacc tgcggctggg
     1381 atgccagggt ggtcaggtca tcccggtaca gggtaatgtc cagaatccct accggcacct
     1441 tgatgccttc gatctcctcg attttttcag cgaggcgctg ggccagcggt cctccccggc
     1501 ggcggatgcc aatcagggcg actttttcca cccctttgtt gctttcgatg atctcatgcg
     1561 caatgcgggt aatggtacgc ttgacagccg tttcgtccat gagtttcttt ttggtttcca
     1621 gtctcaagct ctcaacctcc ctctggccgc tctataaaaa tagaaaagcc tgcactccaa
     1681 tcgcagaggc aggcacagaa atccatcatt ccttgctagc ctctcggggc caacttaaag
     1741 gttctgaacc aattctaatc aatgccagcg cttgtgtcaa acaatattat tccccccgcg
     1801 caagttgggt gaggatgtcc tggaaatatt gcgggagagg gctggtgaac tccatatatt
     1861 cgccgctgcg gggatgaacg aagcccaaca gacgggcgtg cagcgcctgg gagtcaagat
     1921 tgaaatgttt gcgcgccgag ctgtaaagcg gatcccctac cacggcgtgt ttgatgtaag
     1981 caaaatgtac ccggatctga tgggtgcgcc cggtcagcag cttgactttg accaaggtgt
     2041 agttgggata tctttgcagg acttcatagt cgctgatggc ctcccgcccg tccttgacca
     2101 ccgccatctt tttgcggtcg gtggtgctgc ggccgatggg tgcatcgata cgacccaaat
     2161 tttccttgat gatcccatgc accagggcaa tatattcccg gttgatgctg tgcacccgaa
     2221 tctgttcggc cagactgtga tgggcgcggt catttttggc caccaccatg accccggagg
     2281 tgtccttgtc caggcggtgt acaatgccgg ggcgcagttc cccgttgatc cccgacaggt
     2341 ccttgatatg atagagcagg gcattgacca tggtcccgtc ccagttgcca tgagcgggat
     2401 gcaccaccaa tcccttgggc ttgtcaacca ccaccacatc tccatcctga taaataatgg
     2461 gcaggtcaat attctgcggt accagttcta cggcctgcag agccggcacc tccacctgca
     2521 cggtgtcgcc ggttttgacg cgataactgg ccttggatgc ccgtccctcc acccgaattt
     2581 ggcctgattt gatcaaattc tgtaccactg aacgggacaa gtcttcgagg ctgtcgctga
     2641 caaaactgtc cagccgctcc ccttccattt cttcggttac caacagcgtc tcaaaatccg
     2701 gcataacctg gctccttttc catcattaat acaactatta tatccgatca acccaacaaa
     2761 tccaaaacaa cgcaaacatt aacctcagac cagcgttgcc tttatagagc gcaagtttag
     2821 aacgatcccc accttagcct aatggcagag ccgggcgatg gcgcagccga gcccttttgc
     2881 gaacgcaagg caagaaccgt ccccaacttg cgtggcttgc gttattgggg gaagcccatg
     2941 ccgccggggc ctttgccgcc atggccttgc ccgccgccgc ggggtccgcc aagggctttt
     3001 tgcacggcat ccgcttgttc ctgggtcagg gcgccggcac tgaccagcac agccagatca
     3061 ttgccccggg tgggttgatt ggcctggaaa taggcctgtc gttcttctgc ggtcatggct
     3121 ttaacctttt ccatttccga ctgacgttcg gcctgttccg cttgtatggc ggccagaatg
     3181 gcgtcagact gggcggaagt gatggtaccg tccgcaacca gggcacttag ttgatcggtt
     3241 gttctgcgct gcatttcctc ttggcgctgg gcttgctgat tgttgcggat ggcatcagcg
     3301 gtgtcactgc tgatgatttt ttcatcgacc agcatggtca acaaatcttt tttgacggct
     3361 tccagatcgt cggcggcggt tttacgggct tcgaaataag cctgccgttc ttccgcggtc
     3421 atggccttga tcttgtccat ttcggcctgc cgttccgcct gcagggcatc gattttggcc
     3481 tgaatggcgg tcgattcgtc actgctgatg accccctccc cgaccagcgt ttgcaggttg
     3541 agattcatgc ccatttcccc gcccatcatg ccgtgaccgt gtcctcttcc gcccggcatc
     3601 atggattgga atttggtttt cacattgtca atggtattgc tgacggaact gctgtcggcg
     3661 gcccagacat agcctgccga gcccagcaat aagccgccta gcaaggcgcc ggtaacaact
     3721 tttttcagcc agggtttttt attcatatca ttcatccttt cttttcataa aacttgtttt
     3781 ccgggtatgg ttttagtgta aacagcgatt gtgaagtttc catgaaaaga aaatgaagat
     3841 tataaaaatg attgattcat ttttattgag gaaggaatat tggcaggggg atcattggtt
     3901 aattctcatc atagcgatcg acttcgaacc agaattcaac cccgtcggcg cagttcagga
     3961 caccgtaatc gttgtgatgc tgctgcagga tggcgcgcac aattgacaag cccagaccgc
     4021 tgccgccggc ggcccgggtg cgggccttgt cgaccttgta gaaactgttg aaaattttgt
     4081 ccagcgactc attcggaatg ggctggccgg aattgtacac ggcgatgcgt acttgctcct
     4141 cgccgatgtg gagcgtgatg gccagctggc ggcgctcgtc catgtagttg agcgcattgt
     4201 tgagataatt gatcagcacc tgctcaatcc gcatcatatc agcataaacc ggcagttcac
     4261 cttcattttg ttcccagttc aattgcggca caacggattt ttccgtaaaa ataggctgat
     4321 atttgctgat cacccgggcg agcaaatcga acatatcaaa caccctgcgt tcaatcttga
     4381 aggtccctga ctcgtactgg gacaaatcga gcaaatcgcg caccatcaag ttcatcttgt
     4441 gggcttcatc ggttatgaca tcacaataga agtctttgct cacttcgtcc tcgttgacgt
     4501 tgagcttcaa gccttcggca tagccctgga tgagggcgat gggggttttt aattcatgcg
     4561 agacgctgga gacaaattcc ttgcgcattt catccagact gcgttcatac tcaatatcgg
     4621 cctgcagctt gcgattggct ctggtcagtt cgctgatggc cttgcccagc tgatctgaca
     4681 gggagttgat gctgtggccc agttcgccga tctcgtcctg gctggtgacg ggatattttt
     4741 cgctgaagtc cagctgcgac attttctggg caatctgatt gagtccgcgg atgggacggg
     4801 tgaaacgccg ggcaaagaaa aaggccgcca gcaaaccgat caggatggtg acaaggccgc
     4861 tgaaaaagaa gaagcggttg gcaatggcag cgctctcccg aatggcggac aagggggtcg
     4921 ccaggaccag atagtctccg ttgtccaggc gggtaaacaa gttcaggaag tcaatcgaca
     4981 ggcggtcatc ttcgatcaac gcgaaaacgt catgttgctc gagcaccgcg cggttgtttc
     5041 tgatgtagtc cagacccgct tccagccggc catccagacg gggtacgggc aggccgccca
     5101 ggctgggccg ctgtccataa acaggatatt tcaggacgaa tgcttcatcc aaaataatca
     5161 tactcagggc agattgactt tgcagtcttt ccatctccag gatgatgtcg tccggctccc
     5221 cctggtaaat ggcttcaatc tgccgggcgt tctgcagcag ctggcttttc ttgtggttca
     5281 tatagtattg ttccagatag ttgctgttga ccagcaggga caagagcaca aaacaggtca
     5341 ccagcaggct gatcaacagg aacaaacggg tttgtacgga tttgatcatt ttctgccctc
     5401 aaatcgatag ccgatgccgc gcacggtttg aatacaatcg cccatttcgc ccagtttcag
     5461 gcgcagattc ttgatatgga cgtccaccgt gcgcatgtca ccgtaaaagt cataactcca
     5521 gaccgcattg acgatctggt cacggttcag cgcccgccct tcatggtcgg ccagataaac
     5581 cagcagttca aattccttgg gggtcagatc gatgacttcc ccgttgacat aaacataatg
     5641 ggcgttttca tcgatgacca gtcctccgat ggtgcggggc ggatagttct ttccttcggt
     5701 acgccgcagc agggcattga cccgggcaat caaaataggc gggctgaacg gtttgctgat
     5761 atattcatcc gcgcccagtt caaaacccat caattcgtcg gcttcctggt cacgagcggt
     5821 cagcatgatg atcggtatcc gcgacgtttt acgcacttcc cggcagactt cccagccgtc
     5881 catcaagggc atcatcacat ccaggatcaa cagactgaga tcgtcggtct gctgaaaaat
     5941 gtccagcgct tccttgccgt tggccgcttc caggatggca tagccttccc gtttcaaaaa
     6001 atcgctcagc agtttgcgca tgcggtcttc atcatccgcc aggagaactt ttttggtcat
     6061 caaaaaccct gcctttatca cttaaaatgg catattcatc cgcttacatg atacaacctg
     6121 attgtgaaga ttatgtgaat ttactcggtt ttttggccgg tttccagcag atacagccac
     6181 agcaccagca aagtcccgcc gcagacgatg ccgcaatccg ccagattaaa aaccggaaac
     6241 cagcccacac tgataaagtc aactaccgaa ccgtacatca cccggtcgat taaatttccc
     6301 agcgctccgc cggtaatcag ccccaaagca tactgggcca tgggcggcag atggtattta
     6361 aacaggaaat aaaccgctcc cgcaatgacc accgccgcga tcaccacaaa taatagtccg
     6421 ccgccctgca tgaggccaaa agccgcccct tcattatgta tatagcgaag ataaacgagg
     6481 tcacccatga tggcctgacc gtctccgaca ttcatattct gcataatcca ccccttgctg
     6541 aaacgatcca aaatcaatac aaatcccgcc gtcagccaga aacgcacgtt cattcctcct
     6601 ttattgccta tggtaacata ttatcaagat aagatataat aatactgttg catggcgcac
     6661 acaaaattat tttttcctat tcgggccagc atatttccta taacagcgca gctttcaatg
     6721 ctgtgcagcg tggccagcca acccttaaaa acaggtttct acaacgaaat caaggttgca
     6781 ttgtcaatgc caaaccagcc ttgtattttt catccggaag atattcatca ggtggcgcat
     6841 gccaatggcg aaaccatgat gaattaaaaa tattttttca aggggtggat taattgaatt
     6901 tgcaggaaat gtacaaaaag aaactgatgt cagccgagga ggccgtcaaa ctggtcaaaa
     6961 accataccga agtgtggatc ggaattgccg tgcccgtccc gctggcgctg gtcaatgccc
     7021 tggtggaccg ggaacccgaa gtcgaggata tcaccatcaa tcatatcgtc gatattcatc
     7081 cccacaacac ctggcataaa ctcgaccgcg acaccaacat ccgggtggat tgcggctatc
     7141 cttccgccag tcgggacaaa gtcgtctgcg gtgacttcac catatctcct attcgttttc
     7201 atgaaatgcc caagatttat accaatgaaa acttccggcc gatgcatgtt gccatgctca
     7261 acgtaacccc catggaccaa cacggctatt tctgtctggg gctgggcgca gataccaccg
     7321 tggccattgc ccagaatgcg gcccgcaaac gccgcgccgg ggacgaccgc tataccgtgc
     7381 tggcccaggt caaccagcag gttccccgca gccggggtct caactacatc cacatctccg
     7441 aagtcgatgc cattgtggaa caggatcagg atctggcagt gttgccggaa tccgggcccc
     7501 tgtccccgga ggaaaacacc atcggccatt actgcgcgga attcgtcaag gacggttcca
     7561 ccctgcagct gggcatcggc agtatcccca atgcggtggc cctggctttt ctggaaaccc
     7621 atgttcgcga cctgggcatt cattcggaaa tggcctgcga caacatgatc gatctgtggg
     7681 aaaatgaagt ggtgaccaat cgccgcaaaa cctttatgcc gtatcgttcc atctttacct
     7741 ttgccatggg caccaaaaaa ctctatgact ggatcgatga taatccggga gtggaattcc
     7801 accccgtcaa ttttgtcaac aacccgcata tcatcggcaa gaacgacaac atggtctcca
     7861 tcaacgcttg tctgcaggtc gatctcaccg gccaaatctg ctcggaatcc atgggttaca
     7921 aacagtatac ccatcccggc ggacaactcg actttgtgga tggagctttt caatccaaag
     7981 gcggggtttc catcatcgcc acgccagccg tagcctaccc gcgcagcaac aacggccagc
     8041 ctgtttccaa gatttccccg cagatcaccc ccgggggcat catcaccacc ccccgctcct
     8101 gcgcggatta tgtcgtcacc gaatacggtc cggccaaaat caaaggccag tcggtcagac
     8161 agcgggtaaa aaacattatc aatgtggccc accccgattt ccgggatgag ctgcgctttg
     8221 aagcccgccg ccgggatttg atttaaccgt cctgcacacc tgccgtcccg tgggctgagc
     8281 atttaactgc cgggtggtct ggcggttggc ggacggcatg atcaatcgtt gcaccgttag
     8341 cgggccgttt tgtcgcatcg tttggctggt cggttggctg gttgtacagt cacccggcat
     8401 catggacgga tgataacacc tgcctggcaa caaaaaaagt gtccggagca aaccttctct
     8461 ggacacttta tcatttttat ttcctccgac cttgcttgcc cagccttttt ccttggctgc
     8521 acagaaacac cggacgtgaa tcttagccgc acgatgcgtt gataaaaatc tccacgatgc
     8581 ggggatcaaa ttgcgtgccg gcgaattgtt tcagttcctc cagcgcctcc tcccgggaga
     8641 cggcatgacg gtaaacccgg tcagaggtca tggcatcata ggcgtcaatg accgacaaaa
     8701 tgcgggacag cagcgggatg ttctccccgg ccagaccttg cggataaccc gacccgtccc
     8761 agcgctcatg atgactgagg atatattctg cgatgggcat caattcatgg gtggacatgg
     8821 caatgcgaaa gccgatttcc gggtgtttgc gcatttcctt ccactcttct tcgttcaagg
     8881 gtgtttcttt gttcaaaacc cgtcgatcta tccccagctt gccgatgtca tgcagcgtcg
     8941 ccagcaattc cagtttgccc atctcatcat tggaaagctg cagcctggcc ccaatccggc
     9001 gtgacatttc caacagccgg ttggcatgcg cctcggtttc gtgactgatg ttgaacatgg
     9061 tggatttcat ggcggcaatg atggaaccat gggaactctt gtgttccagc agcttacgcc
     9121 ggtacatgat gtcttcagcc gacttgatga cctggttgat atcctctgac caagattctt
     9181 tggtatcgaa ccccagggag acgctcatat aattctcttc ctggatgcgg aactcgtccc
     9241 ggaatgccga ttcgatttgc ttgaacaggg cctgggcctc ttcccggctg gtgttcggca
     9301 tgagaatggt gaactcgtcc ccgccggtac gcgccagcat atcaccctcc cggcagcagg
     9361 ccgccataac ccgggccgtt tcccgaatca gctggtcccc aatctcatgg cccagggcat
     9421 cgttgatcag tttcaagccg ttgatatccg cgataatgac cgataaaggc aggttttcag
     9481 cgcgatccag acgcagcttc tccgcttcaa aatagcgacg attgtagagt ccggtcaaat
     9541 aatcatgcgc gttggtatac agcaggctgc gttcactttc ctgcagttcc ctgatcatcc
     9601 gatagcgttc agtgatatct tcgagcaggc acaaatactc ccgcaccaga ccctgctcgt
     9661 caaaagtcag cggggcaatg atcattttta cccataccgc cttgccatcc ggtttgatga
     9721 atcttttgtc catctcataa ccatcgtttt gcccggcttt aaactgcgca aacagtgcca
     9781 ggtcttgcgg caaatcgtcg ggatgggtca tttccttcca attgagggca gccaattccg
     9841 taaaggagcg gccgagaatc tgctcaaact gctgattcat caccgagata aaacgaaagt
     9901 cgctgacaat tgcgatgccg atcggcgctt tttcaaaaac ccggcggaac agttcttcac
     9961 tctcacccag tttgcgggtc atcaccgcca gcttgtcccg ttcctgacgg agaagttcgt
    10021 tgctgacggt tttgcggtaa taggcgttaa atgccaacaa aagcccgatc accagggccg
    10081 ccgcatatcc tgtattgagc aaaggttcgg cataccattg ccaagccgga taatcaatgc
    10141 caaaagcggc cagcgtctgt ccgctgtgcg gattgatgac cggaacggta accgtgaccc
    10201 atgttcccca ttgatccttc accagcgggg tgataatcga ttctccttgt cgcagccggt
    10261 tgaaatcctg ctctgaatac ccgtcatacg cctgcccggg aggagaatag gccggtgact
    10321 ccggtggttc ggaatccgcc agaaaataaa cgcggccatc atgatacacg aagagatagg
    10381 caaaacgcat tttcccgttg cgctcgacaa aggtagtcag ttcctccttc agagcggaat
    10441 aggcaggttt gtcaatgtcc gcgggggtgg cctccagctc gtcaaggatt tccagcccga
    10501 taaaagcctc gaaggcctgg gccagattaa ttgcctccgt ttcgctgcga tgaatataat
    10561 gcagataggt gtaggcaata aaaagcaatc cggccagcag aacaagcacc agatacagtc
    10621 tgaacctgac ccgggctggt tgatttttat tgaaattttc tgcagaatga gccgtgcctt
    10681 gacgcttagg gcggttcatt gttatgaaac agtccttcct gatcaaatgt gaaaaaccac
    10741 aggtagtaag aaaatataga acgaacgttg acaggtacag taaccggaaa aaccctggta
    10801 cttataggaa agcctcttta gttggcaaag tcaatattta aaagttcttc atcctcgagc
    10861 tccaacatgg cggtcggccc cagccaggtc agggtaagcg tccctttgtc ggccaggtcc
    10921 cagttggttt gctggatcgt gagggaaggg acaaaggaaa agcgcggcgg attggccaga
    10981 cgggcccggc tcaatccctg gatcgtgtcg gccagacagg cggagccgcg caccgagacg
    11041 atcagatcag gccggtttaa atgaccgaaa atatcctctg ccaccagcgc catgcgcacc
    11101 gccagctgca aatgcagaca ttcctcctgg tgatgggcaa tggcccggcg cagcatttgg
    11161 ttctggcggt attcgcggtt ttttcccggg atataggaag tgacgggaga aaagacaatg
    11221 tcgttttcaa aatagggctt gatatcgatc accggcgttc cgtcgacagc gtcgagtttc
    11281 tccaggaaca gccgatggcc ctcgcgtttt atcaatttgg tcagggacat ggcgactgga
    11341 ttgggacggc tggcagcccg cagggcaaac actccgtatt caggcaaatc acggttgaca
    11401 ccgggcggag tcaccagatt gctgcggtct gattgatgaa accaggatag gatccagagg
    11461 tgtgaattct cttcaatgcg cagcagcgca gcttcatacg gcggcaggat ttcaatgacg
    11521 gcaggcacgc cgccggctgg catctccttg gctgtcttca cggatgaaac cacccggcct
    11581 acaggattga gacagatatt ctccaactgc cctctccccc tccagaagat ttgcttttaa
    11641 tcataataac atatgattct cccggtgaca gcagaatgat gtaattttat tcaatcccgc
    11701 cattattttc agaacattca ataagtttat gcgtcaggcg tattaactta tggaattttc
    11761 tgattattcg tcttcgatga tgaggtggag gttgctgttg gacgcggcgg cttcccacaa
    11821 tagttccagg agagcgtctc cgtatgcgcc caaattggcc agcatggctg gcagattagt
    11881 gaaatagacg gttttttcag agaaatcgct gaccagacaa tcccacaagg cgtccagatt
    11941 gccgccgtag taatcgggca gggacagggc ttgctggaga tgacgatggg tggtttgggg
    12001 gtcggtcatg acagcgccgt tcaggtaaaa ctcctgcata tttatacttc ctcctttata
    12061 ttacgtatta cggaaatggc cgggaataat cgtcaccccg cagctgatga aaccaggctc
    12121 gctccagcca accatgctgt atcagataat ccggacgcca tcccgttgcg ggaagttctg
    12181 ccaatggtgg ttgttttggc tgattcccgg tcttgttgcg gtttaatccc tggtggatgg
    12241 cgccgcggtt taatacaatt gctgaaaggt ctggtaatgg tcagcggaat aaaatatcaa
    12301 gccgtcgttg gaatagacga tgcgttccgc cccgcggtaa ccgccccggt aattgatgtc
    12361 gcactcatag tattggcggc ccgaagcctt gggcaaaaag ccttcccggt tgccgaaacg
    12421 atcaccgcca atcgacattt gttcggtgac ttcccagaga ttgccctcgg cattgttcca
    12481 gccgagctgc tgcgcttctg ccttggtgag ataattcggc ggcagttcat gatactgatg
    12541 caggtacgcg gccactgctt ccggagtgga atagcgctgc cctgcctgta cggtaactgc
    12601 cgctgtttcc gacgagggtt ggccggcctc tgtttgactg gtctgctgcg gcgcaggcgc
    12661 agatggttgc tcataaacgg gtgcctgaca gccggacaaa catgccagtg ccagcaggag
    12721 ggcgatcata atggcccatg accggattgg tttgcgttgc aacatgcggt ctccttttgg
    12781 atacgaattt ctgcttacga tccttgcttc ttggactata tataaaaaaa gcgcaggcgt
    12841 caaataccag tgttcataca agggcacatg agaacgcaca aggtgcgttt gtatctgtgt
    12901 tggcaataag gcagaccggc caagcttagg cttcggcggc ggtgatggag tctgcggctg
    12961 aatcggttgg aaaacggacg aagaaagccg tgccctgggc accggtttca taatcgatgt
    13021 gagcccgatg gcgggcggca atgctgtaac aaaccgacag accgaggccg gtaccggatt
    13081 ctttagtggt gaaaaagggc gtgggcagtt tttccgctac ttcttccggt atcccggttc
    13141 cttgatcccg gacatagagc accacttcac ggccttccac acaagtcccg atggcgagct
    13201 caccatgaga ccccatggct tccagcccgt tgcgggccag gttcaggatc aactgacgaa
    13261 tctcattttc atagagatgc agcagcggta cgttgcccaa ctccagatga atactgatct
    13321 cgcgttggtt ggcatcggat ctgatcatgg gagtcagcga agcaatgatt tcatccagcg
    13381 aaacctggtc cagaaacaac aatttatccc gggccattcc caaataatcg ctgatgatgc
    13441 cgttggcgcg atcgatctcc tcgatcataa gatcaaaata ctccccatct tcctggtatc
    13501 tttcatagtt gcgcatcatt tgcaggaatc cccgcaccga agtcatggga ttgcggattt
    13561 catgactgat gctggcggcc atctggccga tgatatttaa acggtccagt ttctcgagtc
    13621 ccgcttccat tttcttttcc cggctgatgt cccgcatcat gcagatgcgg cacagctcat
    13681 tgtccagata gagggccata gaagaagcag aaacagtaat cagctgacct gattttttcc
    13741 tgatccggta ttccgccgta tgctcctggt ttatttcctc cagttcacgc cttattgagt
    13801 gattggtcaa gggatcgttt tcccaaatac ccaattccat gggggtccta ttgatgatct
    13861 ctgcgcgcgt atagcccatg caatcgagaa atcgctgatt cacatccaaa taagtgccgt
    13921 cttgcatact gatgacactg atcatctcag gactgttatg aaaaagcagc tgacagcgct
    13981 tttccgactt ttgcagctct tccatcagct ggtagtgctg cgtatggtca atgctcgtct
    14041 ccagaaccag gtcagcaccg tccgtgtcca aaaagggtat attatatact tccacctgtc
    14101 ggccgtcggg ggttgtcatg tgccagcgat gcggctgacc ggtctcgaaa actgcaaacg
    14161 tctcacagta cgcacagggc tgattcaagc cgaaaatgta ttcataacag gcttgtccgt
    14221 tggactcacc gtgctgatct cgaaatgccc gattggcaaa aatcactttg tggtccggcg
    14281 caaacaaaga gacatgaaaa ggcagactgt ccaatatggc aaacagcctt tgtctctctt
    14341 ttgataattc cactgttcgg ttgatgaatt tctccgccag ttcaccttct gttttcttta
    14401 attgctcgat cgtctgcagc agccaggttc gattcttttc caattctgtg ttgcgttggc
    14461 ggaggttgtg caattcttgc agcagttgat catgcttcac tggcaagcga tccattttgc
    14521 catcctccaa cctgatttgt atagaccgta ttttcgtatt gacgctaatt tgatgattct
    14581 ctaaagaata aaaatattcc tgcaaatcat atttattgta ttttactgta tattttatta
    14641 atgggaagta tttccattat tataaacacc ctctggcaga cggacgcgca ccaagttatt
    14701 tgccagtgga gcaggaaaaa gatcaatttg tattgaagta tttattgatt ggcaggaata
    14761 ctggaggaaa taaaatgctc aatgatatta tcataacggt tctgatcctt ttaggcggga
    14821 ttgcgattta ctttgccctg ctgggcatga tcatactgta caaccgttat atcgctgccc
    14881 cttctcaccg gcagcccctc atgacggaaa ggactcgccc ggaagatgtc cccccggcag
    14941 aagctgattt gcaatcccca acaggtgaaa tgcaggcaga ccccttgccg gattatgccg
    15001 actttgcgga cagacagact gaacgccatc tgcccgagcc aggccggaca ggattaaaac
    15061 tgccggcagc cggattgccc aaatgtaata tccccaaact caacctgccc aggcctgccc
    15121 gcacaaaaaa acgaacccag ccgccggaac cggtcgaccc ggtgcgcagg gagctaaaaa
    15181 aacaggaaga agccttccgc cgccggatgc agcagcgaga tgaagaattg ctgcaggaac
    15241 tcaaacagcg ccgggaggag agtcgtgttg ccgggcaacc tgacagcagc acggagcctg
    15301 ttcatcccac gggaatgtaa cgcccaaaat ctgctgcaac accgtcaaac ttttgtcggg
    15361 aagcaatcaa ccgctggcaa ccacagctca gcctcgctct actattatta tacggctggc
    15421 atctgcctgg ctggagtcag gccctgcatt tctttttcgg tttgcgtctg cgtgactgga
    15481 gcccgtcacg tcatgcaacc tctgtcatcc ctgtaaagat acgagcctgc gccgctcaaa
    15541 cggtatcgaa gagcccaccg gtcggggaat gctgacgcct tctcccgccc gccatcaatc
    15601 ctgtttacaa acaccgcatc tttatgtgat aatgttcacg ttatgatttt gcacgtgagg
    15661 tgacaggatg aattatcaag agatgtacca gcgcaaagta gtatcggtgg aagaagccct
    15721 gaaccagatc caaagcggac aggaaatcgc ctgctccctg atcggctgtg aaccggtgac
    15781 catgctcagt cagctgcata ccatcaagga ccgggtagag gatgtttccg tgctgaccgc
    15841 cttgctgatg aacgagtacg agttttttat gaacccggac atgaaaggtc attttctgct
    15901 gaacagctgg ttctataccg gcggcgcccg tcaggcccac ccgctgggaa ccgtctctta
    15961 tgtccccctg gaactgcacc gctgcatatc cagccggatg gcgtatcgcc gccccgacat
    16021 tttcctgggc tgcgcctgcc ccatggataa acacggttat ctgtccctgt cgatggacac
    16081 cgtttgcgaa aaagaactgc tggaaaacgc cgcccaggtc atcctggaag tcaatcccaa
    16141 cctgccccgg atcggtggcg acaccctggt gcacatctcc caggtcgatt atattctcga
    16201 aactacgcgg gcacttccca ccctgccgga agtgcagctg aatgacaagg aaaaacaaat
    16261 cggcgcttac gtcgccgaac tgattgaaga tgaatccacc ctgcaaatcg ggtttggcaa
    16321 tatccccatc gccgtcaccc gctgcctgac cgacaaaaaa gatctcggcg tacataccga
    16381 gatgttcagc gaaggcctgc tcgatctgta tgaatgcggc gcgctgacca accgcaaaaa
    16441 gaccctctgg cgggacaaat tcgtggccga ctgcgccttg ggcagccagc gtctgtatga
    16501 tttttttgac aacaacctgg cggtcgagtt tcatcgcggc agcgtggtca acgatccccg
    16561 cgtgattggc cgcaatcaca aaatggtggc catcaactcc gccctggaaa tcgacctgag
    16621 cggacagtgc tgcgccgaat ccataggcag ccgtcactac agcggcaccg gcgggcacaa
    16681 ggaattcggc cagggaaccc agtattcccc gggcggcaaa tccatcatcg cgtttctgtc
    16741 gaccgcccgc agcgatacca tctcccacat cgtgccccag ctgcagcccg gcgccgtggt
    16801 caccacttcg cgcatcgatg tggattatgt ggtcaccgag tacggcattg ccagtctcag
    16861 aggccgctcg gtccgggagc ggatcacgga actgatcaac atcgcccatc ccaatttccg
    16921 agactggctg cgcagcgaag ccgagcgcaa tatgatgtgg taaactgagc aaaataacaa
    16981 attccccctc atggaaaatc cccggctttt tttagaaggc cggggatttt taatgtttct
    17041 gcctctggtt gctgaacgaa tatcgaatgc gtttattcac tttttaatgg gccaatcggg
    17101 gggcatgcgt cggacgctcg cgctgtggaa cggttcttgc ttccatcagc ggagaataac
    17161 ccagcaagga cgtagccagt tcaatcgtgc cgatcacgcc gcacagggtc gccggccaac
    17221 cggtaatcac ttccgtcccc gtcagaataa acaacaccag acaagtcagg aatctggcta
    17281 ttacttcatt tttgtttaac attataacac ctgctttcca tttatttgcg tgattaaact
    17341 gatatttatt taatcacact catatttaaa gcaaatgttg taccacatta aagtaatatg
    17401 catgttattt tacgcacaat ggaaagcgcg gggttttatt ctgtttttat gtttaatatt
    17461 cactgaaaca gaaacgaata agcgtaacac atttgtgtta atagcgaaca atattgtgac
    17521 aacttcgtga caacattatg attttgggtg attatagggg actgtccccg aaaatcattc
    17581 gaactggttg agcgccacca agactggatc ccacaccggg ctgaagggcg gtgagtaagc
    17641 cagatccatg tcgatcaagt cctcgatggt ggcgccgacg gtgatggctg cagccagcat
    17701 gtcaatgcgc atgcctgcgc cggtatagcc cacgatttgc gcaccgatca ggcggcggtt
    17761 gctccggtcc atgatcaatt tcaaatgaat atcccgggca atcgggcaaa agtgcgccgc
    17821 ggtgcgggag gaaattcgct gggtaacata gtcaatccct gcttgccggc attcgtgttc
    17881 actgaaaccg gtccgggaga tttccatctc cagcaccttg gcaatgccgg tacccagcac
    17941 gcctttaaag gaagcgttgc cgccggcggc attttcaccg gccactttgc cctgtttatt
    18001 ggcggtagtg cccatgggaa tatatacttc attgcccgac agcagatgca aggtggtggc
    18061 acagtcgccg gccgcataaa tgcccggcag gttggtttcc attttctgat tgacgcgaat
    18121 ggcatttttc acgcccagtt caatgccggc cgcggcagcg atttccgaat tcggcaccac
    18181 gccgatggag agcagcacaa agtctgcagc cagttcgccc tggtcggtga tgacaccggc
    18241 gacccggccc tctccaaaga agccgttgac cgcttccccg gtacgcactt ccaccccttt
    18301 ggcctccagg tattgggtca ggatctgggc catgtcttcg tccatgttgg ggatgatatg
    18361 cggcgcccgt tcaatcacac aaacctggca gcctctttcc gtcagattct ccgccatttc
    18421 cagaccgata tagccgcccc cgatgaccac cgccttgtgc ggtttatgat tttctatgta
    18481 ggcttttatc tgcagactgt catggatatt gcgcagggca aaaacgcctt ccaggtccat
    18541 gccttccagc ggcggcacca gtgcccgcgc accgctggca ataaccagtg catcatagtg
    18601 gtcctgcgtg gcgctatggt tttgcaaatc ccgcagcacc accgttccct cttccggttt
    18661 gatttcctcg gcccgcatca tcgtccgcac gatgatgttc tggcgggcaa actcctgcgg
    18721 tgttcgcgcc accagtttgt cctcgctctc cacccgtccg ccgatgaaat agggcaagcc
    18781 acagcctgcg taagaaatgt attgatcatc cgtgtaaacc acaatgtcca tatcccgttg
    18841 gatgcgccgg gccttggccg cagcactcat cccggccgct acgccgccga taataaccag
    18901 tttttgcgtc atggtcatgg ctcctttcgc gtgttgtaat cagtgaatca tttttcgaat
    18961 tacttgtatt gtatccgata aacaacgtcg cgtgaagtcc gccatgaatc attggggatt
    19021 acgattgggg tctgacctcg tttgcctgca gtgctttttt gatttcgtaa agcaggtcga
    19081 ccagatgatt gcagccttcc ggaccgccag tcagggcagc aatttctttt ttgttcattt
    19141 cccgaagatt caggccgggc agtttgaaca gatgggcatc gttctgaaaa cagatcgggt
    19201 ccggggcgtt gataaaattg ccttgcgcag agagcaccac cccctgtggg tcaatctcca
    19261 acgagacgcc cagatggtga aagctatcca tgaaggaagc gctgacgagg cggttgacgg
    19321 cctgttgggt gatggatacg gttttgcagc gattgaaaag gttgtaatcg cgctcggttg
    19381 agcccagcca ctcggtccat tgcagttcgg tctcttccag atgactgtag aagcggcacg
    19441 ccccgatact atgctgttcg caaaagtcat catattgctt ggccgacaca aaaccccgtt
    19501 ccaggaaaaa ataggtctcg gcctgcagca cgccccgaaa gcattcgctg atcaaatcat
    19561 agggcagctc cagcccgggc ccacccagca ccttgcgcaa gaccggtccg ccggtgatat
    19621 aggcttcgac gccaatcagc gcaggcaggt caaagacgcc tgtctcccct gccgatgaac
    19681 ggtaattggc ccaacccgcc cgactgatct gcaaggtctg cacactggca tcgacccagg
    19741 caaccgcttc gaccgcattg ctgagcaggg tgcattccgc cgtgactgtg tccccatgac
    19801 gattgacttt gacaaaattg gtggcatgaa ataataaaga catcgatacc ctccttgaaa
    19861 tttgggtgcc aggcacctaa ctttctaact tttgaaggga ttgtagttta ccccgtaatc
    19921 aaacgtgtcc aggccttcct tgcgtttcag ccagccaacc accgcgtatg tcaagggggt
    19981 ggcgagcatc tcgtaggtta ccttgaacaa atactggaac agcatcatct gcaaaagaac
    20041 cgccgcagga actgttccca ggaaggacag ggtaataaac aacactgtgt ccatcccttc
    20101 ccctaccagg gtagagccaa tcgttctcat ccagaggaaa cgtcccccgg taaacaattt
    20161 cattttcgat aaaacgattg cattcaggaa ctcgccggcc acatacgcca gcagcgaagc
    20221 catcacgatg cgcggcgcca ttcccagcac cagcgcaaat gcttcgccgc ccgcaaaaaa
    20281 agctggtgac gggatggcca tcaccagata aaacactccg accatcagca gattacagac
    20341 aaagcctgtc cagattaccc gccgcgtctt ttgaaaacca tatacttcgg tcaaaacgtc
    20401 cccaatgata taggccagcg ggaacaaaat caccgccccc ggcaacacca caccggcaat
    20461 cagaataagc ttgccggcaa taatattgga caacaacaaa ctgctgacaa aaagacaggc
    20521 gataaccaca aagaaatctg acgtgccttg cttttcctgc atctgacgtt ccaccttcac
    20581 cgcttcattc gtattaatct aattcaacta ccctagtagt atagtatttt cagcacaggc
    20641 cggcaagtgt tacgccggtt acagcataac actagatgac tttattgccc cggcccagtg
    20701 ttttgactgt caatgaacca tcctgggtat tgaataccgc tgtccggcca tagtttgccc
    20761 cgatatcctg ggccacgatt ctgattttga gccgctgcag ctcctgcgta acggtttcaa
    20821 tatttctatc gccaatgctt tttccttcgc tgacaaacat attggcaccg ccggcaattt
    20881 tcgcagtcag atgcaggtgc gcacagcctt gctttatcat tccccggacc agttcttcga
    20941 tcgccgtatc ggcaaatttg taaacatttt tcccgctcac atcgccaaat gcttccggca
    21001 gcaagatatg ggccagaccg ccaatccggc gcagacggtc ataaagacag atacatacac
    21061 atgaacccag cgcatacgtc gtgagggtgt caggcggacc ggcaactttt tgatcggata
    21121 tgcccaccac caaattcctc ataatgacat gcccaggctt tcaaaaatca ggctcaggga
    21181 atccggttcc ggcataatga gcaaatggct tctgatagta ttttcctcgc ttaaaaaatt
    21241 ttcctcaata aacagcaggt tgtcatccat cacgccaaaa taggcagcgg gataattaag
    21301 gatggccccc accatgtcaa tggccacctg gggtgtggag agccgaatgt caagatcggt
    21361 catcgtggca atggcgctga cataggaacc tgccagcaca ttgccgattt ccatgatgac
    21421 ggacatatcc atttccgaaa tatcagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnngcca gcacattgcc gatttccatg atgacggaca tatccatttc cgaaatatca
    21601 gcaaattcat taatgtcctt gctgaggaga acattgagca gcagacagac aaattccttt
    21661 tccagcagaa acagcaacat gccccgcaca tcgcagttca tggtcactaa aattcccacc
    21721 atttcatttt ccgggccgcc caggatattg gccatgctgt ttacgtcaat aatctgcagt
    21781 tcggggaccg acatcataat cgtatccgcc ataaaatcag ccagcgcggc ggcggcgttg
    21841 cccgcgccaa tgttgccgat ttccgagagc gcactcagct gatattcgtt gaggttaaga
    21901 taatttccca tgaaaagtca tcccttcaag taacgttccg aagcaattga gggcacaata
    21961 acgcaaactg ttataaattt attacctgat atctatatcg gtaactatcc agtatttttt
    22021 agtactgtta cgctgggtcg ttcttttctg taacaattgc ggatgcccct ccacctcatg
    22081 gtgagctttc gcggcggggc tgagtctgct atacttagcg ttcgttttaa aaatgctgac
    22141 agaaaaaaca gggggcagag aaagttagaa agttaggtgc ctggcaccta acttgtaact
    22201 tggttgcatt ttcacaagaa tcagtctata ataaatatac ccccaagggg tattatggag
    22261 gtgaagttta aatggaattt gtactgaatg agaaaacgct aactgccata aaagaagata
    22321 ccggggcttt attcctcaag ataatggaat gcgtttcttg agcgggtacc tctatcagcc
    22381 tctggtcagg ggtatcgaat cctaaaaaag aaaacctttc tagatttaat gaatacgaac
    22441 accaaggggt caagatctat attgaaaaga gattaaaaac ccaggataca attcatattg
    22501 atgtaaaccc caaatttctc tttttcaaac cctcctttag agtccggggc gtagaggtgc
    22561 caaagcacca aactttctaa cttacctggt aaataaattt gtaaagtctc ctgttactga
    22621 aaaagcgcag caggagacat tttttaaaag ttaatgacaa gggaatccct tgtcactctt
    22681 agcaaggcaa gaaccgtcc
//
LOCUS       DAOTHZ010000034        22406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_2736_length_22406_cov_7.619391, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000034 DAOTHZ010000000
VERSION     DAOTHZ010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 22406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22406
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_22406_cov_7.619391"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="P5549_05595"
     CDS             <1..240
                     /locus_tag="P5549_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW12579.1"
                     /translation="RWPFFIEGILLGSVGALLAVLLLALGYGTLVDNVQTTMFFVPLV
                     NDHHELLMIYLTLFGTGALLGCLGTIISLNRFLDV"
     gene            330..1466
                     /locus_tag="P5549_05600"
     CDS             330..1466
                     /locus_tag="P5549_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRW12580.1"
                     /translation="MRSNHRIAATFVALCFMLTMILPVYADEISDKQRQLEEVSRQIE
                     AQNQKLTTVKKKENSILGQVQNMEKDMQKTQSDLEYLSGRVDYLQSSLAVTRDEIGIL
                     EAELEKQNEILNQRLVFIYEEGDISYLEVLLGAEDMQDFLVRYDLLNRILQQDRDLIT
                     TVQAKKEALSSKKNALENQKAELEDNQQSHKQKQKQLDEQIDEKKEILSDVQKEKKAF
                     QRALAELEASSRQLEAMIQQMQSSGEPDKAGTGTFSWPVPGYNTITSDYGMRYHPILK
                     VNKLHTGIDIGVPSGVNILAADSGTVIYSGWMGGYGQVVVIDHGSGLSTLYAHQSTIL
                     ISNGKAVNKGQVIGKVGSTGWSTGPHLHFEVRKNGTPVNPHSYI"
     gene            1632..2783
                     /locus_tag="P5549_05605"
     CDS             1632..2783
                     /locus_tag="P5549_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRW12581.1"
                     /translation="MFLKNNKLLTAIFSFFALLGLLGFLALAFIFVTNINGLGSLLTV
                     VGLLQSESLYDTQPEKMIEGATAGIVSSLEDPYSKYLNKDEWENLELQLNAEFGGIGV
                     YVVELEDGRLGIVSPIKGTPAARAGLKNGDIIIRLNDQSALQMTQDEAVHLMRGEPGT
                     QLDMEVLRPSENKEYEFKIIREIINVPSVEYEVKGDAIPIGYVKLNQFHAHSTQEIAD
                     SLNRLNEEGIKGVILDLRDNGGGDFEASIAIADIFLEDQAVVSVKDAHDNVTVHRAEA
                     GSLKLPMVVLVNNNSASASEILAGALKDNKRAVLVGEKTYGKGLVQTVFQLRDGGALK
                     LTTQKYFTPDGTDINKIGINPDYIVKNSETEGQDLQLERALQLIKGQIL"
     gene            2800..4041
                     /locus_tag="P5549_05610"
     CDS             2800..4041
                     /locus_tag="P5549_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRW12582.1"
                     /translation="MNWVATVMQMMGQVLADTFTSPFFLALWGMIMLLVWWQNKRQHR
                     LSASFIHDYPDYSWQAALFSSFLGLLGGLAGSILLVFLGIDLGSISITALWLTALLLM
                     MIRPRFICFAYAAGVIAVCNLVWGVPHTSIPQLIGLVAALHMIESLLILLNGTFHPPV
                     VYIKRHGQLCGGFNLQYFWPLPLLALISVGFPGTTPSLEMPAWWPLLRDYSSLSGQQV
                     FTIVPVMAVLGYGEICTSRTPAQASRRSALLLFLFSLLLLALAVFSVRIPALLWLAAF
                     FSPLGHELVIWLGMREESRAPLYIQAAQGVKIMEVLRGSSAQKVGIKPGDTLLQANGS
                     QIESFVQLQELLRNHRLPVALRIKRAEHFIAMTWPPGRQRGRDIIPVPEPDTGTYLVW
                     QEDRYFHVARLLWRKLKGFLA"
     gene            4116..5192
                     /locus_tag="P5549_05615"
     CDS             4116..5192
                     /locus_tag="P5549_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12583.1"
                     /translation="MQVNSRGLIRYVFASSHTTQGYCTFIPELIQDLEQVYILKGAPG
                     TGKSTFIRLIGEALSDQGLEVEFWISALDTITPDGVYIPQLNLAVINGSMQISIEPRY
                     PGVKEHLIHLGDYWDQAALAAQRQQIIAWVDKRIHAQQKADDLLKKLGRLKAEQRQLY
                     SQQINPERIRKLIKQLGHEIVENRSGEKHYFAGVLSIDGWVDYVHELSHGCQKRYIFK
                     GPMGSGKSMVIAELACLVKENGYFLEYYHCGLEAENLVMVIIPNLQIALVESGDGDIP
                     LKPWDVIVDLGLYLEGDFDENLPEKNAYSRNLENLLLEARQELAVGSSCQQELKKIYA
                     GAMDFANLDQLRRDLLETIRPSQP"
     gene            5314..7302
                     /gene="uvrB"
                     /locus_tag="P5549_05620"
     CDS             5314..7302
                     /gene="uvrB"
                     /locus_tag="P5549_05620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRW12584.1"
                     /translation="MKPFQLHSTYSPSGDQPQAIRELVEGLKQGCRDQVLLGVTGSGK
                     TYTMAQVIQAVQRPTLVMAPNKTLAGQLYSEFKEFFPENAVEYFISYYDYYQPEAYVP
                     QTDTYIEKDSSINDEIDKFRHSATASLLERRDVIIVASVSCIYGLGAPQDYYEMAISL
                     RPGTMMPRELLLRRLVENHYERNEMTFFRGAFRVRGDVVEVFPAASWDKALRIELFGD
                     EIERIVEFKPMTGEITGKLMHAMIFPASHYVTTRENMLAAAEEIENELAQQLSQFERE
                     GKLLEAQRIGQRTRFDLEMIKEIGYCKGIENYSRYITGRKAGEPPYTLIDFFPPDFLL
                     FMDESHISVPQVWGMYAGDRSRKENLVNFGFRLPSALDNRPLKFAEFDSKINQVVYVS
                     ATPGDYEMEHSQHVADQVIRPTGLLDPEIEVRPTRNQIDDLMGAIQLKVEKGFRVLVT
                     TLTKRMAEDLADYLDDAGIKVRYMHSDIEALERMEILRDLRSGEFDVLIGINLLREGL
                     DLPEVALVAILDADKEGFLRSGRSLIQTAGRAARNVEGKVIMYADRITPSMQQAIDET
                     ERRRQKQIAYNQEHGITPETIKKAVFASLEATIAEEPADYTAVEWKKMSSEERNRLIK
                     EMETEMMQAAKGLDFERAAQLRDMIREMQTSKKTKKRR"
     gene            7317..10145
                     /gene="uvrA"
                     /locus_tag="P5549_05625"
     CDS             7317..10145
                     /gene="uvrA"
                     /locus_tag="P5549_05625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRW12585.1"
                     /translation="MQDYIYVKGAREHNLKNIDLNIPRNKLVVFTGVSGSGKSSLAFD
                     TIYSEGQRRYVESLSTYARQFLGQMNKPDVDYIAGLSPSIAIDQKTTSNNPRSTVGTV
                     TEIYDYLRLLFARVGKPYCPHCHRPIQRQTVDQVVDTVLGLQEGSKIKVLAPIIKAQK
                     GEHRKVIEGLFKDGFVRLMVDGYEYSSEEEIKLEKNKKHDISAVVDRLIIKADIRSRL
                     AESLELAFEMGNGMVKIIVLDTTGEDEALIFSRDFACPECGFSLPELSPRMFSFNSPF
                     GACPECDGLGEKKEIDPDLILNKEKSLEDGAIIPWSGAFHNYYRQLVAGMAKAYKIPL
                     DKPVKDLSAKQIETILYGNGGERFKIEYTNSYGEKRDFHSAFEGIVNNLARRYAETRS
                     DASREDLEKYVTSSPCQRCQGKRLREEILWVLLGSKSIAEVSSFSIRESLEFFTVLQL
                     NERDMTIARQVIKEIKTRLGFLMDVGLDYLTLDRAAGSLSGGEAQRIRLATQVGSSLM
                     GVLYVLDEPSIGLHPRDNDRLLATLKRLRDLGNTVIVVEHDEETMHNADFIVDIGPGA
                     GVHGGEVVAAGSLDDIIHSEDSLTGQYLSGQRDIAVPLQRRPGNGHELIIRGAREHNL
                     KDIDVSIPLGKMVVVTGVSGSGKSTLIEDILYPALMKKYYGGRHKPGQYKEMIGAEYL
                     EKVINIDQSPIGRTPRSNPATYTGAFDGIRELYTNTTESRIRGYSAGRFSFNVRGGRC
                     EACSGDGIIKIEMHFLPDVYIPCEVCKGKRYNRETLEVKYKGHNIADVLAMTVDEGVD
                     LFKNITKVHRKLKVLQDVGLGYIQLGQPAPTLSGGEAQRVKLATELSKRSLGKNIYIL
                     DEPTTGLHKEDIKHLLEVLDRLVETGNTVVIIEHNLDVIKSADHIIDLGPEGGENGGR
                     IIAQGTPEELMLQPQSYTGLYLRRVLQS"
     gene            10170..11984
                     /gene="uvrC"
                     /locus_tag="P5549_05630"
     CDS             10170..11984
                     /gene="uvrC"
                     /locus_tag="P5549_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRW12586.1"
                     /translation="MEALRERLKHVPLQPGVYLFKDQADRVIYVGKAKILRNRLRSYF
                     QSPQHLLPKVKAMMMRVADFDYVVSGSEVEALILENNLIKAYQPRYNILLRDDKTYPY
                     IKISADKYPRIAMVRESKDKVSRYYGPYTDVGNIRETLKLINSIFPLRTCKNLREQTR
                     PCLNYDLGKCLAPCSGQVSEADYQLIVEGMINFLEGRSPDLLVTKKAEMHAAAAHLDF
                     EKAARLRDQIKAIQALDARQQVVFDHPYCLDAIAMIAGEKENLALVFKIRKGQVIAKD
                     TYWLKQAMEEAKGEVMDFFLKHYYGENQDIPDIILLNEAPAEQQLLENWLKTISGRAG
                     HFSIPIRGEKKRMMDMVAENARLLWEEKTQQDLKAHRILQHLAEKLELEVLPDRIECY
                     DISHLAGEETVGSMVVFTGGREDRKAYRRFKLSRDQNDDFASLREVLERRFTEARQGN
                     PAFLPEPDLLLIDGGLGQVNAVKRILTQMGVETAVVGLAKKNEEIFYPGQGEPIRLVR
                     RDEGLMLLQRLRDEAHRFAITYNRQRREKKVTRSILDEIPGIGPQRKKALLRQFGSVA
                     RLEQAELAEITAVPGMNRAVAQKVYDHLHQPKQNEKRT"
     gene            12313..12669
                     /locus_tag="P5549_05635"
     CDS             12313..12669
                     /locus_tag="P5549_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRW12587.1"
                     /translation="MQGWLIRWICNIFAIILTAYIIPGFELTIWGAIVGSIFLGVINA
                     VIRPLLLLLTLPLNVLTLGLFTFIINGFMLYLTSATIKGFTIHGFWWAVLSALLLSVI
                     SFLISYLIKDKMFHLK"
     gene            12922..13725
                     /locus_tag="P5549_05640"
     CDS             12922..13725
                     /locus_tag="P5549_05640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRW12588.1"
                     /translation="MTIKMVALDLDDTLLNSQLKISPACVRAIRAARQKGVIITLSTG
                     RMFQSALPYARQLEIDVPIITYQGAWVKSSLSGEVLYYQPVPAGLARQVMLFFRQQGV
                     HYHCYYNDELCIESSSAEADDYACLAGVDARMYDDLITALGHYDTLKIMAISTNESRL
                     LEMEKEMKARFADQLYITRSKPFYLEVMNRLAGKAQALALIADHLGIQPAEVMAIGDS
                     FNDLDMIKWAGWGVAMGNAIASVKEAADFVTFSNEEEGVAEALQRFVLQ"
     gene            13772..14893
                     /locus_tag="P5549_05645"
     CDS             13772..14893
                     /locus_tag="P5549_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRW12589.1"
                     /translation="MASKVYFANLRINSGSLLDRAEKVLTQAGFAKIIAPRDLVAIKL
                     HFGESGNLAYIKPPYVRRVVEMIKKLEGRPFLTDANTLYVGTRANSVDHLETAIQNGF
                     DYAVVGAPLIIADGLTGKDYVKVKINGDHLKEVNIASNAYHADAMVVMSHFKCHELTG
                     FGGALKNLGMGLGSRSGKQQMHSDVLPSVFDKKCTGCAKCMEWCPADAIAMQAWDGNK
                     KGRKAVIMADKCWGCGECIVSCKFGAIRPNWKTRPEASQEKMAEYALGAVQNKADKTI
                     YINFVTDISPECDCISHNDFPIVQDIGFLASTDPVALDMACVDMVNRMPAMPGSVIEG
                     LAAGEDKFRAVHPDIDWLPQLVHAEKIGLGSRQYELIGK"
     gene            14909..15913
                     /locus_tag="P5549_05650"
     CDS             14909..15913
                     /locus_tag="P5549_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRW12590.1"
                     /translation="MQDFIVAVDIGGSKILGAVLDRSGQILLRRREATAAIGKPDVVV
                     GQVAGMITEMKNEMGLQEDDILGIGVGVPGPLDFRTGVVEGSPNLKWEKYPLREKLRS
                     RLGSHLWLDKDTNAAVLGEYYYGLTPLCGHLIYVTVSSGVGGGIITDGQLLHGINGGG
                     GEIGHMVVAPEGDICGCGRRGCLEVMASGWAVTREANALIARGSGNGILSHVRPGQAV
                     GPREVGMAARQGDSVSLQLIERTGSYLGMAIANLVNIFNPAKIVLGGGMALGLQDLLL
                     PIIQKKVSSNVFTLHRRDLYIEMTRLGEDIVLAGCAAMVLQETSTVNECNRKGFSDDD
                     "
     gene            15903..16925
                     /locus_tag="P5549_05655"
     CDS             15903..16925
                     /locus_tag="P5549_05655"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRW12591.1"
                     /translation="MTIEKMQTYLYDLPEQFSQMLGLKIELPGRYQKDYRQIVVSGLG
                     GSAIGGDILRSYALNRSRIPVIVNRDYVLPAFVNEHTLVMAVSYSGNTEETLSAYRQA
                     KQNGADIIAVSSGGRLSELAREDGNMVIAVPGGMSPRAASGYLFCPLALTLEKMGLLP
                     DARRDLQETGQILRELREELNPNVPADNNPARRLAKQMKDRIPVIWGSAGHSEIAALR
                     WKAQINENAKAPAYCNAFPELNHNELVGFEAPAELLGRLVVIILCDPDDHVQVKKRIH
                     ITSNIIKAKVADTIEIKTRGGDWLAKLYSLIYVGDYASFYLALEYGINPTPVKVIDYL
                     KAQLAQ"
     gene            17072..17896
                     /locus_tag="P5549_05660"
     CDS             17072..17896
                     /locus_tag="P5549_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRW12592.1"
                     /translation="MRFYGDYHTHSRYSDGRQSVRDIAAAASKKGIREVAVTDHGPHV
                     AVIGVKQEETYLQIKEEAQAVQADIDVTVFVGAEANIKSLQGELDISPAIITRLDVLI
                     AGLHPYTRPSGIEDGLGIWMQNSLRHFGKGQQEKARNNNTKATVEALYRNPEIDILAH
                     PGLFFKVDMEETARACIKNEVCFEINCGHKHPELSDIMIVNKLGVTFIVNSDAHFTDT
                     VARLDYGDRIVGQLDIEPERIANRWQGGEYNENQREQRSTFAHYYRDVRRGQNPGH"
     gene            17817..18704
                     /gene="rapZ"
                     /locus_tag="P5549_05665"
     CDS             17817..18704
                     /gene="rapZ"
                     /locus_tag="P5549_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRW12593.1"
                     /translation="MKTKESSDQHLLIITGMSGAGKTQAINCLEDLGYYCVDNLPPSL
                     LIKFIELSIQAETRLVKVAMVIDVRGGEFFHDLFRSLNELEENRISYEILFLEASDDV
                     LVSRFKESRRRHPLAQGGVLLEAIRTERKMLEEVRGRANIIVDTSNLTPRELNAKLSS
                     YFSENKTGDFNINIVSFGYKVGIPVDCDIMMDVRFLPNPFYDPIMRNMTGEDQEVIDY
                     VLQSPVSKSFIRRYLNLLKFLIPYYIAEGKTNLAISIGCTGGQHRSVVLADHIAQQLR
                     KIGYNAMVRHRDVARYKQE"
     gene            18723..19709
                     /locus_tag="P5549_05670"
     CDS             18723..19709
                     /locus_tag="P5549_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRW12594.1"
                     /translation="MLRSNNEFKIVVVGGGTGLSVLLKGLKQYTDQLTAIVTVSDDGG
                     SSGRLKTELGVLPPGDIRNCLVALAETETLMDKVFQHRFKQGQGISGHNLGNLLMVAM
                     TEITGDFVSAIKEVSKVLAVRGQVIPATLESVNLGAEMIDGTVIIGETAIRQYRKSIA
                     RLFLIPEKCLPIQASLDAIMAADAIVIGPGSLYTSIIPNLLVEGVSDAMMRSPAVKIY
                     VSNIMTEQGETDNFNAFDHIKTILQYAQQPIIDYAILNTGTIDELRLQRYHQEQAIPV
                     DTSCQQIQAMGIKTVTEDMVAQDDLAWHDTRKLAQAILRIVQQENSAGPASG"
     gene            19749..20687
                     /gene="whiA"
                     /locus_tag="P5549_05675"
     CDS             19749..20687
                     /gene="whiA"
                     /locus_tag="P5549_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRW12595.1"
                     /translation="MSFSNEVREEAARIISEKSCCKRAELAALIAMRGETTHREGMVS
                     LQLESESAVAARKVYRLLKDVCGLQASTSLVERKRFKQKRFYLSQADLSDDELQQMKK
                     LGLLDDGGHVVKYLDPKITARNCCKRSYLRGLFMCKGFVNRPEANYHLEIVFNDTRMA
                     KEVKKLIGRLNLETGIGERKNNLIVYIKDSEKIVDFLRLIGASKALLEFENVRIVKSM
                     RNQVNRQVNCETANLGKTIDASVRQVELIEALIDQLGMNKIPVHLRELALLRIEHPDS
                     TLKELGTLMSPPLTKSGVAYRMRKLEIFAEENLYGP"
     gene            20799..22199
                     /gene="rpoN"
                     /locus_tag="P5549_05680"
     CDS             20799..22199
                     /gene="rpoN"
                     /locus_tag="P5549_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRW12596.1"
                     /translation="MKLSHDIYLDQQQKLLMTPELRQAIAMLQMSTLELGQYVQNELE
                     NNPFLEEKDADDHEELETNAEKLDEVQADNKSEEWLEYYNDRDIGLTVADVEEKSFDA
                     FVSKSPSLYDHLQFQLHMTICDESELMIGEYILGSIDANGYLTVGLEELVQATGTDLE
                     RVQHMLTIIQSFHPHGVGARDLAECLLIQLEHYGKDDPIATAIIEEHLQDLGKGRINK
                     IAQQLGVGVQQVQEACDFIRSLDPKPGLQYSNGDDTKYIVADIILEKVDGEYVVIVND
                     SGYPHLMINRLYEKMIRNPESFSPEARKYMEEKMGSAVWLIKSIEQRRLTLYKVARCI
                     VDIQKDFLDRGIKYIKPLNLKQVADLVEVHESTVSRATSNKYIQTPQGLFELKFFFGS
                     GISSYANANCDKVSSKSIKKQIQELIEKEDSFNPLSDQQIVEIIKQQGAVISRRTVAK
                     YRQELGIQSTVARKRY"
BASE COUNT         6051 a         4820 c         6008 g         5527 t
ORIGIN      
        1 cgctggccgt ttttcattga aggaattctg ctgggatcgg tgggggcttt gctggcggtc
       61 cttctgctgg cactgggtta cggtaccctg gtagataacg tgcagacaac catgtttttt
      121 gttccgctgg tgaacgatca tcatgaattg ttgatgatct atctgacctt gttcggtaca
      181 ggggctttgc tggggtgttt gggaacgatc atctcactga atcgattcct ggatgtataa
      241 aaaacctcaa tcaataaagt aaatatcagc gatttttctt gacgaattaa cattcttttt
      301 atattctggg gcttaaggag ggggatattg tgcggtcaaa tcatcgaatt gcagcgactt
      361 ttgtggcatt gtgctttatg ctgacaatga tcttaccggt ttacgccgat gaaatctctg
      421 ataaacagcg gcaattggaa gaagtcagcc gtcaaataga agcccaaaac caaaaactga
      481 cgacggtaaa aaagaaagaa aacagcattt tgggtcaggt acagaatatg gaaaaagata
      541 tgcagaagac ccagtctgat ttggaatatc tgtccggccg ggtggattat ctgcaatcca
      601 gtctggcagt gacccgggat gagattggaa ttttggaagc agagctggaa aaacaaaacg
      661 agattttgaa tcagcggctg gtttttattt atgaagaagg agatatctcc tatttggaag
      721 tcctattggg ggcggaagat atgcaggatt ttctggttcg ctacgatctg ctcaaccgca
      781 tcctgcaaca ggaccgcgac ctcatcacga cagttcaggc taaaaaagaa gcgctgagtt
      841 ccaaaaagaa cgctttggaa aaccagaagg cggaactgga ggataaccag cagagtcata
      901 aacaaaaaca aaagcagttg gatgagcaga ttgatgaaaa aaaagaaatc ctcagtgatg
      961 tgcaaaagga gaaaaaagct tttcagcggg cgctggcaga actggaagca tcatcacgtc
     1021 aattggaagc catgattcag caaatgcaga gcagcggtga accggacaag gcaggcacgg
     1081 gaactttttc ctggccggta cccggttaca atacgattac ctccgattat ggcatgcgtt
     1141 accatccgat tcttaaagtc aacaagctgc ataccggcat tgacattggc gtcccctccg
     1201 gagtcaacat tctggcggcc gacagcggaa cggtgattta ctcgggctgg atgggcggat
     1261 atgggcaggt tgtggtcatt gaccacgggt caggtttatc tactctgtat gcccatcaat
     1321 caacgattct gatcagcaac gggaaagctg tcaacaaagg ccaggtgatt ggcaaggttg
     1381 gcagtactgg ctggagcacc ggaccacatc tgcattttga agtacggaaa aacggtacgc
     1441 cggtcaaccc gcattcctac atttgatgaa tcagcctcag caaggagtca agagcattgc
     1501 cctcttgact cttttttcgt ggtgctggat gagcaggaag aaaggaatca atttccataa
     1561 taatttgtgt tggttctaca attgcggtaa catattaaag atgaaaaggt aataataatt
     1621 aatatggtgg tgtgtttttg aaaaacaata aattgttaac ggcaatcttt tcattttttg
     1681 cccttcttgg cctgctggga tttctggcat tggcttttat ttttgtaacc aatatcaacg
     1741 gtttgggcag tctattgacg gttgttggct tgctccaatc ggaatcatta tatgataccc
     1801 agcctgagaa aatgattgag ggtgctactg ccggaatcgt atcctccttg gaggatccct
     1861 attccaaata tttaaacaag gatgagtggg aaaacctgga actgcagttg aatgcagaat
     1921 ttggcgggat aggggtttac gttgtggaac tggaggatgg ccgactggga attgtttctc
     1981 ctattaaagg cacaccggca gcccgagcag gtctgaagaa tggcgatatc attattcgtc
     2041 tcaatgatca gagtgctctg caaatgacac aggatgaagc ggttcacttg atgcggggtg
     2101 agcccggaac gcaactggac atggaagtcc tgcgcccctc tgaaaacaag gagtatgagt
     2161 tcaagatcat ccgggaaatc atcaatgtac cttccgtgga atatgaagta aagggagatg
     2221 ccataccgat tgggtatgtc aagctcaatc agtttcatgc ccattccacc caggaaattg
     2281 cggatagtct gaatcgtctc aatgaagagg ggattaaagg cgtcatactg gatttaagag
     2341 acaatggcgg cggtgatttt gaagcctcca tcgctattgc cgatatcttt ctggaagatc
     2401 aagcggtggt cagtgtaaag gatgcgcatg acaatgtaac cgtacatcgt gccgaagcag
     2461 gcagtttgaa actgcccatg gtggttctgg tgaataacaa tagtgccagt gcttcggaaa
     2521 ttttggcggg tgcgttaaaa gacaataaaa gagcggtact ggtaggcgaa aaaacctatg
     2581 gcaaaggact ggtgcagaca gtttttcagc tgcgtgatgg aggagccttg aaactgacca
     2641 cccagaagta ttttacgccg gatggaacgg atattaacaa aatcggtata aatccggact
     2701 atatcgtcaa gaattctgaa acagaggggc aggatctaca actggaaaga gctttacagc
     2761 tcattaaggg acagatcctg taaacctgga ggtgggtttt tgaactgggt cgccacagtg
     2821 atgcaaatga tggggcaagt attggcagat acctttactt cccccttttt tttggcttta
     2881 tgggggatga taatgctgtt ggtttggtgg cagaacaagc gacagcacag attatccgcc
     2941 tcatttatcc atgactatcc ggattattca tggcaagctg cgcttttttc atcctttttg
     3001 ggactgttgg gtggtttggc cggaagcatc ctgctggttt ttttggggat cgatctgggc
     3061 agtatcagca ttactgcctt atggttgacc gcgttgctgc tgatgatgat tcggcctcga
     3121 tttatctgtt ttgcctatgc agccggagtg attgccgttt gcaatttggt ttggggagtg
     3181 ccccatacca gcataccaca gttgattgga ctggtcgcag ccctgcacat gatcgagagt
     3241 ctgcttatcc tgctgaatgg cacctttcat cctccggtgg tgtatatcaa aagacatgga
     3301 caactatgcg gggggttcaa cttgcagtac ttctggccgc tgccgctgtt ggccttgatc
     3361 agtgtgggtt ttccaggtac cacgccctca ctggaaatgc ctgcgtggtg gccgctgcta
     3421 agagattaca gcagtctgtc cgggcaacag gtttttacca tcgttccggt catggccgtg
     3481 ctgggttacg gggaaatctg cacgagccgt acgcctgcac aggccagtcg tcgatctgcc
     3541 ttgcttcttt ttctcttttc cctgctgctc ctggcactgg cggtattctc agtccgtatt
     3601 cccgcattgc tttggctggc cgcttttttc agtcctttgg ggcatgaact ggtgatctgg
     3661 ctgggaatgc gagaggagag cagagcgccc ttatacattc aagcagcgca gggagtgaag
     3721 attatggagg tattgcgcgg gagcagtgcc caaaaggtcg ggatcaagcc gggcgatact
     3781 cttttgcagg ccaacggcag tcagatcgaa agttttgtcc aattacagga gttgctgaga
     3841 aatcatcgac tgccagttgc tctgcgaatc aagcgggcgg aacacttcat tgccatgacc
     3901 tggccgcctg gccgtcaacg gggcagggat atcattcctg ttcccgaacc ggataccgga
     3961 acttatttgg tctggcagga ggatcgctat ttccacgtag cccgtttatt atggcgaaag
     4021 ctgaaaggct tcctggctta gcgcgagcac ggatatcttt gttcaccttt ggttgacgtt
     4081 ttgaatatag taaagcaaac ttgaggagaa gagggatgca ggtgaacagc aggggactga
     4141 tccgttacgt tttcgccagc agtcatacga ctcagggata ctgtacattt atccctgagt
     4201 tgattcagga tttggagcag gtctatattc tcaaaggagc ccccggaacc ggtaaatcaa
     4261 cgtttattcg tctgattggg gaagctcttt ccgaccaggg actggaggtc gaattctgga
     4321 tctccgccct ggacactatc actcccgatg gggtgtacat cccccagctc aatctggcag
     4381 ttatcaacgg cagtatgcaa attagcatcg agccccgcta tccgggggtg aaggagcatc
     4441 tgattcattt gggtgactac tgggatcaag cagctctcgc agcacaacgt caacagatca
     4501 tcgcctgggt agacaagcgt attcatgccc agcaaaaagc ggatgatcta ctcaaaaaat
     4561 taggccggct caaggcagaa cagcgccagc tttattccca acaaataaat ccagaaagaa
     4621 tcaggaaatt gattaaacag cttggccatg aaatcgtgga aaaccgttcc ggtgaaaaac
     4681 attattttgc aggggtttta agcattgacg ggtgggtcga ttatgtgcat gaattaagtc
     4741 atggctgtca aaaacgctat atttttaaag gaccgatggg cagcggcaag agcatggtca
     4801 ttgcggaact ggcctgtctg gtcaaagaaa acggctattt tctggaatac taccattgcg
     4861 ggctggaagc cgagaatctg gtcatggtca ttatacctaa tctgcagatt gccttggtcg
     4921 aaagcgggga cggagacatt cccctcaagc cctgggacgt aatcgttgat ctgggtttgt
     4981 atctggaagg ggattttgat gaaaacctgc cggagaaaaa tgcgtacagc aggaacctgg
     5041 aaaacctgtt gctggaagcc cggcaggaat tggccgttgg cagttcctgc cagcaggaat
     5101 tgaaaaaaat atatgcggga gcgatggatt ttgccaatct cgatcagctc aggcgggatt
     5161 tgcttgagac gattcggcca agtcagccat aggtccaaaa ttaaaaagga atgcagactg
     5221 ccttgcttgt cagggcagtt attatattat agtattataa aaaaaagagc atctgttcgt
     5281 tttaaaatat acccatgagg gggaacctgt ctgatgaaac cgtttcaact acattcaacc
     5341 tattcaccat caggggatca gccccaggcc atccgggaac tggtggaagg tttaaagcaa
     5401 ggctgccgtg accaggtttt actgggcgtt accggttccg gcaaaaccta taccatggcc
     5461 caggttattc aggcggtgca aagaccgacg ttggtcatgg cccccaataa aacgctggcc
     5521 ggacagctgt attcggagtt taaagagttt tttccggaaa atgcggtgga atactttatc
     5581 agttattacg attactatca gccggaggct tatgtgccgc agactgatac ctatatcgaa
     5641 aaggattcct ccatcaatga tgagattgac aagttccgcc actctgccac cgcttccctg
     5701 ctggagcgcc gcgatgtaat cattgtcgcc agtgtctcct gtatttacgg cttgggcgca
     5761 ccgcaggatt attatgaaat ggccatatca ttgcgacccg gtaccatgat gccgcgggaa
     5821 ctcctgctgc gcagactggt ggaaaatcat tatgaacgta atgaaatgac ttttttccgt
     5881 ggcgctttcc gggtgcgggg tgatgtggta gaggtattcc cggctgcatc ttgggacaag
     5941 gcgttgcgta tcgaactttt tggcgatgag attgagcgca ttgtggaatt taaaccgatg
     6001 accggtgaga ttaccggcaa gttgatgcat gccatgatat ttcctgcttc ccattatgtt
     6061 acgacccggg aaaacatgct ggccgcggcg gaagagattg aaaacgaact agcgcagcaa
     6121 ctcagtcagt tcgaacggga aggcaagctg ttggaggcac agcgcatcgg ccagcggacc
     6181 cgttttgacc tggaaatgat taaagaaatc ggctattgca aggggatcga gaactattcc
     6241 cgctatatca ccggccgcaa ggccggcgaa cctccttata ccctgattga tttctttccc
     6301 ccggactttt tattgtttat ggatgaatcc catatttccg tgccccaggt gtgggggatg
     6361 tatgccgggg accgttcccg caaagaaaac ctggtcaatt tcggttttcg gctgccgtct
     6421 gccttggaca atcgtcccct caagtttgca gagtttgaca gcaaaattaa tcaggtggtt
     6481 tatgtcagtg ccacccccgg cgattatgaa atggaacaca gccagcatgt ggctgaccag
     6541 gtgattcggc ctaccggcct gctggaccca gagattgaag tgcgtcccac ccgaaaccaa
     6601 atcgatgatc tgatgggcgc aatccaactt aaggtcgaaa aagggttccg ggtgctggtc
     6661 acaaccctga ccaaaaggat ggcagaggat ctggctgatt acctggacga tgccgggatc
     6721 aaagtgcgct atatgcattc tgatatcgaa gcactggaac ggatggaaat tttgcgtgat
     6781 ttgcgcagcg gagaatttga tgtcttgatc ggaatcaacc ttttgcggga aggacttgat
     6841 cttcctgaag ttgctctggt ggcgatactg gatgccgaca aggaagggtt tctgcgctcc
     6901 ggccgctcct tgattcagac tgccggtcgg gcagcccgca atgtggaagg caaggttatc
     6961 atgtatgccg accggattac cccctccatg cagcaggcga ttgatgaaac ggaacgccgc
     7021 cggcaaaagc agatcgctta taaccaggag cacggcatca cgccggagac gatcaaaaaa
     7081 gcagtgtttg cttcactgga ggctaccatt gcggaagaac cggctgatta taccgcagtg
     7141 gaatggaaaa agatgtcttc cgaagaacgc aaccggctga tcaaggaaat ggaaacagag
     7201 atgatgcagg ccgccaaagg actggatttt gaacgggcag cccagctgcg ggatatgatc
     7261 agggaaatgc agaccagtaa aaaaaccaaa aaacgcagat aatctggagg accgaaatgc
     7321 aagactatat ttatgtgaag ggtgcgcgcg agcacaatct taaaaatatt gatttaaaca
     7381 ttccccgcaa taaactggtg gtatttaccg gggtttccgg atcaggtaaa tccagcctgg
     7441 catttgatac gatttacagt gaaggccagc gccgctatgt ggaatcgctt tcaacctatg
     7501 cccggcagtt tctgggtcag atgaacaaac cggatgtgga ttacatcgca ggattatctc
     7561 cttccattgc cattgaccaa aagacaactt ccaacaatcc tcgttccacg gtcggaaccg
     7621 tgaccgagat ctacgattac ttgcgcttgc tgtttgcccg ggtgggcaag ccctattgcc
     7681 cccattgtca ccgcccgatt cagcgacaaa ccgtcgatca ggttgtggat acggtgctgg
     7741 gactgcagga aggcagtaaa atcaaagttc tggcacccat catcaaggcc caaaaaggcg
     7801 agcatcgcaa agtcattgag ggactcttca aagacggttt tgtccgattg atggtggacg
     7861 gttatgaata tagctctgaa gaagaaatca agctggagaa aaacaaaaag cacgacatca
     7921 gcgcggtggt ggatcgcttg atcatcaagg cggacatccg cagccgtctg gctgaatcat
     7981 tggaactggc ctttgaaatg ggcaatggca tggtgaaaat aatcgttttg gacacaaccg
     8041 gtgaggatga agcattgatt ttcagccgtg attttgcctg cccggaatgt ggttttagct
     8101 tgcccgaact gagcccgcgc atgttctcct tcaacagccc ctttggggcc tgcccggaat
     8161 gtgacgggtt gggagagaaa aaagaaatcg atcccgattt gatattgaac aaggaaaaaa
     8221 gtctggaaga cggagcgatc ataccttggt caggagcatt tcacaactat taccgacaat
     8281 tggtagctgg catggccaaa gcatacaaga ttccactgga caagccggtt aaagatttaa
     8341 gtgccaaaca gattgaaacc atcctgtacg gcaacggagg agaacggttt aaaatcgaat
     8401 atacgaattc ttacggagaa aaaagagatt ttcattccgc cttcgaaggg atcgtcaaca
     8461 atctggcccg gcgttatgcg gaaaccagat ctgatgcttc ccgggaagat ttggagaaat
     8521 atgtaaccag cagtccttgc caacgctgtc aagggaaaag actgcgggaa gagatcctct
     8581 gggtattact gggaagcaaa tcgattgccg aggttagttc gttttcgatt cgggaaagcc
     8641 tggagttttt tactgtgtta cagctgaatg aaagggatat gaccattgcc cgccaagtaa
     8701 tcaaggaaat caagactcgc ctgggattcc tgatggatgt cgggcttgat tatctgaccc
     8761 ttgatcgagc ggctggcagt ctgtccggtg gagaggccca gcgaattcgt ctggcgaccc
     8821 aggtggggtc ctcgctgatg ggcgtgctgt atgtgttgga tgagcccagt attgggctgc
     8881 atcctcgtga caatgatcgg ctgctggcga ctttaaagag gttgcgcgat ttgggcaaca
     8941 cggttattgt tgtcgaacat gatgaagaaa ccatgcacaa tgcagatttc atagtggata
     9001 tcggcccggg tgccggcgtg catggcgggg aagtggtcgc ggccgggagt ctggatgaca
     9061 ttatacacag tgaagattcc ctgaccgggc aatatttgtc cgggcagcgg gacattgccg
     9121 tgccgctgca gcgtcggccc ggcaacggtc atgaactgat catccggggc gccagggaac
     9181 ataatctgaa agatattgac gtttccatac cgctgggcaa aatggtagtt gtgaccggcg
     9241 tgtcgggttc cggtaaaagt acactgattg aagatatcct ttatccggcc ttaatgaaga
     9301 aatactatgg cggccggcac aagcccggtc aatataaaga aatgattgga gccgagtacc
     9361 tggaaaaagt tatcaatatc gaccagtcac cgattggaag gacaccgcgc tccaatccgg
     9421 caacctatac cggcgctttt gacggaattc gtgaacttta taccaatacc accgagtccc
     9481 ggatccgggg ttacagcgcc ggccgcttca gttttaatgt gcgcgggggc agatgcgaag
     9541 cctgctccgg cgatggcatc atcaaaatcg aaatgcattt tttgccggat gtttatatcc
     9601 cctgcgaggt ttgcaaaggc aaacggtata accgggagac tctggaagta aaatataaag
     9661 gacacaatat cgctgacgtt ttggccatga ccgttgacga gggagtggat ttgtttaaaa
     9721 atatcaccaa ggtacaccgc aagctgaaag ttttgcagga tgttggcctg ggctacattc
     9781 aactgggaca accggccccg actttgtccg gcggggaagc gcagcgggtc aaactggcca
     9841 ccgaactgtc caaacgctca ctgggcaaaa acatttatat tctggacgag cctaccaccg
     9901 ggttgcacaa ggaggatata aaacacctgc tggaagtcct tgaccggctg gttgagaccg
     9961 gcaataccgt cgtcatcatt gagcacaatt tggatgtcat taaatcagcg gatcatatca
    10021 ttgatctggg gccggaaggc ggcgaaaacg gaggcagaat cattgcccag gggaccccgg
    10081 aggaattgat gctgcagccg cagtcttata ccggtctcta tttgcgccgg gttttgcaat
    10141 cttagctggt gatgatgcag gaggtattta tggaggcttt gcgagagcgc ttaaaacatg
    10201 tccctctgca accgggggta tatcttttta aagatcaggc agatcgggtc atctatgtgg
    10261 gcaaggccaa gattctgcgc aatcgtctgc gttcctattt tcaatcaccg cagcatcttc
    10321 tgcccaaagt aaaggcaatg atgatgcggg tggctgattt cgattatgtg gtcagcggca
    10381 gtgaagtaga ggctctgatt ttggaaaaca acctcataaa agcctatcag cctcgttata
    10441 acattttatt gcgggatgac aaaacctatc cctatatcaa aatcagtgcg gataaatatc
    10501 cccggattgc gatggtacgc gagagcaagg acaaagtctc ccgttattat ggtccctata
    10561 cagatgtggg caatataaga gaaaccttga agctgataaa cagtatcttt ccattgcgga
    10621 cctgtaaaaa cctgcgcgag cagacccgcc cctgtctgaa ttatgatttg gggaagtgtc
    10681 tggcaccttg cagcgggcag gtcagtgaag ctgattacca gctgatcgtg gaaggtatga
    10741 tcaatttttt ggaaggaaga tccccggatc ttttggtgac caaaaaagcc gaaatgcatg
    10801 ctgccgctgc ccatctcgat tttgaaaaag ctgcccggct gcgcgatcag atcaaggcga
    10861 ttcaggcact ggacgcccga caacaggtgg tctttgatca tccctactgc ctcgacgcta
    10921 ttgccatgat cgccggagaa aaggaaaacc tggcgctggt ctttaagatt cgcaaggggc
    10981 aggtcattgc caaggatacc tattggctga aacaggccat ggaagaagca aaaggcgagg
    11041 ttatggactt ctttctcaaa cattactatg gggaaaacca ggatatcccc gacattattc
    11101 tgcttaacga agcgcctgcc gaacagcagc tgctggaaaa ctggctaaag accatcagcg
    11161 gtcgggcagg tcacttcagc atacccattc ggggtgagaa aaagcgcatg atggacatgg
    11221 ttgccgagaa tgcaagactg ctctgggagg aaaagaccca gcaggatttg aaggctcaca
    11281 ggatactgca gcatctggca gaaaaactgg aactggaagt cctcccggat cgaatcgagt
    11341 gttatgatat ttcccatctg gccggagaag aaaccgtcgg ttcgatggta gtttttaccg
    11401 gtggacgcga ggatcgtaaa gcgtaccggc gctttaaact tagccgcgac caaaacgatg
    11461 actttgcgtc tctgcgggaa gtactggagc gaaggtttac cgaggcccgg caaggcaacc
    11521 cggcattttt gccggaaccg gatcttttgt tgattgatgg tggattgggt caggtcaatg
    11581 ccgtcaagcg gatactgacg cagatgggcg tggaaacagc cgtcgttgga ctggccaaaa
    11641 agaatgagga aatcttttat cccggacaag gcgagcccat ccgattggta cgtcgggatg
    11701 aaggcctgat gcttttgcag cgcttgcggg atgaagccca tcgctttgcc atcacctata
    11761 atcgtcagcg gcgggagaaa aaagtcactc gttccatcct cgatgaaatt ccaggaatcg
    11821 gcccgcaacg caaaaaagca ctgctccggc aattcggttc agttgcccgc ctggaacagg
    11881 ccgaattggc tgaaattaca gccgtacccg gtatgaaccg ggcggtagcg caaaaagtat
    11941 atgatcatct tcatcaacca aaacaaaatg aaaagcgaac gtgattccct tgcatatcgt
    12001 tttttccttc attcccgagt atgtggcaac ttcggttgtt gaaaacccaa ctggaaaaga
    12061 tccttttgac cctgaccggc aggcggacac aagcagaaaa gtgtctgaat ggcagacgaa
    12121 gtatcaggta gtcatcatgc gaaacaagag gctttataag aaataatgga cattgcttaa
    12181 aatttcccgg gaaatataac gaatgcatga agaatcgtgt tagaatttta tgaacatatt
    12241 gtgtatcata ttgttaaggg tataataatt gcataccaat gaaaaagtgc agaacaggga
    12301 aggaggcaag ttatgcaagg ctggctgatt agatggatct gcaatatatt tgcaattatc
    12361 ctgaccgcgt atatcatacc tggttttgag ttaaccatct ggggtgcgat tgtaggatca
    12421 atattcctgg gggtaatcaa tgcagttatc agaccattgt tgctgttact gacattgccg
    12481 ctcaacgtat tgactttagg cttgtttact ttcatcatca acggttttat gctctacctt
    12541 acatccgcga ccatcaaagg ttttacgatt catggcttct ggtgggctgt tttgagtgcc
    12601 ctgctgctga gcgtgattag tttcttaatc agctatttga tcaaggataa aatgtttcat
    12661 ctcaaataat tatcatcgtt tgattggatt tggtgaagga agcaatggct gaagcccgcg
    12721 cctgaagcgt acgaccgtac gcagattgcg cgggattttc gcaacaagaa aaagaacagc
    12781 tgcaggttat taatgtccga ttacgggaag aaatcttaag gcgttggcca aggttaccct
    12841 aagacgcacg gaattacatt gagaatacaa caatcttcat caacgcagag attgattttg
    12901 atcattgggg caggaggata tatgacgatc aaaatggtag ccttggactt ggatgatacc
    12961 ttgttgaatt cccaactcaa aatttcaccg gcttgtgtca gggcgatccg agctgcccgg
    13021 caaaaaggag tcatcatcac gctttcaacc ggcaggatgt ttcaatcagc gcttccctat
    13081 gcccggcaat tggagattga tgtgccgatc ataacgtatc agggagcctg ggtgaaaagc
    13141 tccttgagcg gggaagttct atattaccag ccagtaccgg ctggcctggc tcgtcaggtg
    13201 atgttatttt ttcgtcaaca gggagtacac tatcactgtt attacaatga tgaattgtgt
    13261 atcgaaagtt catctgcaga agcagatgat tatgcctgcc tggcgggagt agacgcccgg
    13321 atgtacgacg atttgattac tgcgttgggg cattatgata cgttgaaaat catggccatc
    13381 agcaccaacg aatcccggct gctggagatg gaaaaagaga tgaaagcaag gtttgctgat
    13441 caactttaca tcacccgctc caaaccgttt tatctggaag tgatgaaccg gttggccggc
    13501 aaagcccagg ctctggcgtt gattgccgat catctcggca tccaaccggc agaagtcatg
    13561 gcgattggag acagtttcaa tgatttggat atgatcaaat gggccggttg gggggtcgca
    13621 atgggcaatg ccattgcgtc agtcaaagaa gcggccgatt ttgtaacctt ttccaacgag
    13681 gaagaaggag tggcagaagc attgcagcgc tttgttttgc agtaatttgg ctgcagataa
    13741 aataaattta cgcaaggaga ggttatagca catggcttcc aaagtatatt ttgccaatct
    13801 gcgtatcaac tccggcagtt tgctggatcg agcagagaaa gtattaaccc aggcaggatt
    13861 tgccaaaatt atagcacccc gtgatctggt cgcgatcaag cttcattttg gagaaagcgg
    13921 caatttggcc tatatcaaac caccctatgt acgtcgggtg gtggaaatga ttaaaaaact
    13981 ggaaggacgt cctttcctga cggatgccaa tacgctttat gtcggtacca gggccaattc
    14041 ggttgatcat ctggaaaccg ccattcaaaa tggtttcgat tatgcagtgg tcggtgcacc
    14101 gctgattatt gcagacggtc tgactggcaa ggattatgtg aaagtaaaga tcaacggaga
    14161 tcatttaaaa gaagtcaata tagccagcaa tgcttaccac gcagacgcca tggtggtaat
    14221 gagtcatttt aagtgccatg aactaaccgg ttttggggga gcattaaaga acctgggcat
    14281 gggactggga tcacgcagcg gcaaacaaca gatgcattcc gatgtattac cgtcggtttt
    14341 tgataaaaaa tgcaccggat gtgcaaaatg tatggagtgg tgtcctgctg atgccattgc
    14401 catgcaagct tgggacggca ataaaaaagg acgcaaagca gtgatcatgg cagacaagtg
    14461 ctggggttgc ggcgaatgca ttgtcagttg taaattcgga gcaatccggc caaactggaa
    14521 gacccggccg gaagcatcac aggaaaagat ggccgaatat gcactgggcg cggtccaaaa
    14581 caaggcagac aaaaccatct atattaattt tgttaccgat atcagtcctg aatgtgactg
    14641 catcagccac aatgatttcc cgattgttca ggatatcgga tttctggctt cgaccgatcc
    14701 ggtggcactg gatatggctt gtgtggatat ggtcaaccgc atgccagcca tgccaggttc
    14761 cgtgattgaa ggcctggccg cgggagaaga taaattccgg gcggttcatc cggatattga
    14821 ttggctgccg cagctggttc atgcagaaaa aatcgggtta ggcagcaggc aatatgagtt
    14881 gattggcaaa taagcaggag gcagggtttt gcaggatttc atcgtagccg tcgatattgg
    14941 cggaagtaaa atactgggcg ccgtattgga ccgttccggt cagatcctgt tacgccgtcg
    15001 tgaggccacg gccgccatcg gcaaacccga tgtggtggtc gggcaggtgg ccggcatgat
    15061 cacggaaatg aagaacgaga tgggccttca ggaagatgat atcctgggaa ttggcgtggg
    15121 tgtgccggga ccgcttgatt ttcgaaccgg agtggtggag ggctccccca acttaaaatg
    15181 ggaaaaatat cccttgcggg agaagctgcg cagcaggttg gggagtcatt tgtggctgga
    15241 caaagacacc aatgctgctg ttctgggtga gtattattat ggtttaacac cgttgtgcgg
    15301 acatctgatt tatgtcaccg tcagcagcgg cgtgggcgga gggattatca ccgatggtca
    15361 gcttttacat ggaatcaatg gaggcggcgg cgaaataggc catatggtgg tggcgcctga
    15421 gggtgacatt tgtggctgtg gaaggcgggg atgtctggag gtaatggctt ccggctgggc
    15481 tgtgacccgt gaagcgaatg ctttgatcgc gcggggcagc ggcaacggaa ttctcagcca
    15541 cgttcgtccc gggcaagcgg ttggtccgcg cgaagtaggg atggctgccc gccaagggga
    15601 ctctgtatcg ctgcagctga tagaacgaac cggttcctat ctggggatgg cgattgccaa
    15661 tctggtcaat atcttcaatc cggcaaagat tgtgctgggc gggggaatgg ccttggggct
    15721 gcaggatctg ctcttgccga tcatccagaa gaaagtgagc agcaatgttt ttactttgca
    15781 tcgccgtgat ttatatatag aaatgacccg tttgggggaa gatattgtct tggcaggctg
    15841 tgccgccatg gttttgcagg agaccagcac tgtcaatgag tgtaacagga aggggtttag
    15901 cgatgacgat tgaaaaaatg caaacctatc tttacgattt gccggaacag ttcagtcaaa
    15961 tgctggggct caagattgag ttgcccggtc gatatcaaaa agactatcgc cagattgtgg
    16021 tcagcggttt gggcggatct gccattggcg gggatatttt gcgcagttat gcgctgaatc
    16081 gcagcaggat tccggtgatt gtgaatcgtg actatgtgct gccggccttt gtcaatgaac
    16141 ataccttggt gatggcagtc agctattccg gcaataccga agagacgctc agtgcctatc
    16201 ggcaggctaa acaaaacgga gctgatatca tcgcggtaag cagcggaggc cggctgtcgg
    16261 aattggccag agaagacggt aatatggtaa ttgctgtgcc cggaggcatg tcgcctcggg
    16321 ccgcgagtgg ctacctcttt tgtcccttgg ccttgacgct ggaaaaaatg ggcctgttgc
    16381 cggatgctag gcgggatttg caagaaaccg gacagatcct tcgggaattg cgggaagagt
    16441 taaatccgaa tgtgcctgca gacaacaatc cggcccgccg gctggctaaa caaatgaagg
    16501 atcgcatccc ggtcatctgg ggtagtgcag gtcattctga aatagcggcc ttgcgctgga
    16561 aggcccagat caatgagaat gccaaggccc cagcttattg caatgctttt ccggaactca
    16621 accacaatga gttggttggt tttgaagcac cggcagaact gctgggccgt ctggtggtca
    16681 tcattttgtg tgatcccgat gatcatgtgc aggtcaaaaa gcgcattcac ataacttcca
    16741 atatcataaa ggccaaagtg gccgatacga ttgaaatcaa gacccggggc ggcgactggt
    16801 tggccaaatt gtattctctt atatacgttg gtgattatgc cagtttttat ctggctttgg
    16861 aatatgggat taatcccacg cccgtcaaag tgattgatta tttaaaagcg caactggcac
    16921 aataatggtg tactcaggca taacaaacca aaacctcaaa cataaacagc tcataataag
    16981 gttaaactaa ttgcaaccgg ggagaagcat catccccggt ttcttctttt ctgctgcatg
    17041 atcagcaaat gacaacatag ggggtcgggt gatgaggttt tatggagatt atcatacgca
    17101 ttcgcgctat agcgatggac gtcaatcggt acgggatatt gctgcagctg ccagtaaaaa
    17161 ggggatacgg gaggttgccg tgactgatca tggtccccat gtggcagtga tcggagtaaa
    17221 acaggaggaa acctatttgc aaatcaaaga agaagcccaa gccgttcagg ctgatatcga
    17281 tgtgactgtg tttgtcggag ctgaggccaa cataaaaagt ctgcaggggg aacttgatat
    17341 ttcccccgcc atcatcaccc ggctggatgt gctgattgcc gggctgcatc cttacacacg
    17401 tccgtccggt atcgaagacg gcttgggcat atggatgcag aacagccttc gccattttgg
    17461 caaagggcag caggaaaagg ccaggaacaa taataccaag gcaaccgttg aagcactcta
    17521 tcgcaacccg gaaatagata tactggccca tccgggtttg ttttttaagg tagatatgga
    17581 ggaaacagcc cgggcctgca taaaaaacga ggtatgtttc gaaataaatt gtggtcataa
    17641 acacccggag ctttctgata taatgatagt gaacaaattg ggcgtgacgt ttattgtcaa
    17701 cagcgatgct cattttacag atacagttgc ccggttggac tacggagatc gtattgtcgg
    17761 ccaactggat attgaacctg aacggattgc caatcgctgg caggggggag agtataatga
    17821 aaaccaaaga gagcagcgat caacatttgc tcattattac cgggatgtcc ggcgcgggca
    17881 aaacccaggc cattaattgc cttgaggatc ttgggtatta ctgtgttgac aatctccctc
    17941 ccagtttgct gatcaagttt atcgaattga gtattcaggc tgaaaccagg ctcgtcaagg
    18001 ttgccatggt cattgacgtc agggggggag aattttttca tgatctgttc cgctccctta
    18061 atgaattgga agaaaaccgg atcagttatg aaattctttt tctggaagca tctgatgatg
    18121 tgttggtaag ccgattcaaa gaatcacgcc ggcgacatcc tttggcacaa ggcggggtct
    18181 tgctggaggc catccggact gaacgaaaaa tgctggaaga agttaggggc agggccaaca
    18241 ttattgttga tacgtccaat ctgacgcccc gggaattgaa tgccaaactg agcagctatt
    18301 tctccgaaaa caaaaccggc gattttaata ttaatatcgt atcctttggt tataaagttg
    18361 gcattccggt ggattgtgac attatgatgg atgtcaggtt tcttcccaat cccttttacg
    18421 atcccattat gcggaacatg accggggaag accaggaagt gattgattat gttttgcaat
    18481 cacccgtcag caagtcattt atccgccgat accttaatct gcttaaattt ctcataccat
    18541 actacattgc agagggcaag accaatctgg cgatttccat aggctgtacc ggagggcagc
    18601 atcgctcagt ggttttggcc gatcatattg cccaacagct tagaaaaatt gggtataacg
    18661 caatggtcag gcatagagat gtagctagat ataagcaaga ataactaaag gggcgggctt
    18721 ccatgttgag atcaaacaac gagtttaaaa tcgtcgtagt aggtggagga acagggttat
    18781 ctgtactgct gaagggactg aaacagtata cggatcagtt gactgccatt gtaactgtga
    18841 gtgacgatgg aggcagttcc ggtcgattga aaaccgagct tggagttctc ccgcccgggg
    18901 acattcgcaa ctgtctggtt gcgttggcag aaacggaaac gttgatggac aaggtctttc
    18961 agcatcgttt caagcaaggc caggggatca gcggccataa tctgggcaat ttgctgatgg
    19021 tggccatgac cgagatcacc ggtgactttg tcagtgcgat caaagaggtc agcaaagtac
    19081 tggctgtaag aggacaagtt attccggcta cgctcgagtc agtgaatctg ggagcggaaa
    19141 tgattgacgg caccgtaatt atcggtgaaa cagccatacg acaatatcgc aaaagcattg
    19201 cccgcttgtt tttaattcca gaaaaatgtc tgcccataca agccagtctt gatgctatca
    19261 tggcggccga tgctattgtc atcggaccag gcagtcttta caccagcatt atccccaatc
    19321 ttctggtgga gggtgtcagc gatgccatga tgcgctcacc ggcggtaaaa atatatgtca
    19381 gcaatattat gactgaacag ggcgaaacag ataatttcaa tgcttttgac catatcaaga
    19441 cgattttgca atatgcgcag cagccgataa ttgattatgc gattttaaac accggcacga
    19501 tcgatgagtt gcgtctgcag cgttatcatc aggagcaagc gatcccggtg gatacatctt
    19561 gtcagcagat tcaagccatg gggatcaaaa ccgtgactga agatatggtg gcacaggacg
    19621 acctggcctg gcacgataca cgtaaactgg cacaggcgat cctcaggatt gttcagcaag
    19681 aaaatagcgc agggccagca agcggctgat gtattttgac aaaatggttc ttttcaatgg
    19741 aggctaatgt gagtttttca aatgaggtaa gagaggaagc agcacgcatc atttccgaaa
    19801 aatcttgctg taaacgagca gaactggcag cactcattgc catgcgagga gagaccacac
    19861 atcgcgaggg tatggtcagc ctgcagttgg agtcggagag tgcggtggct gccaggaagg
    19921 tttatcgact tttaaaagat gtctgtggac tgcaggcctc caccagtttg gtggagagaa
    19981 aaagatttaa acagaagcgc ttttatttgt ctcaggcgga cttgtcggat gatgaattgc
    20041 aacaaatgaa gaaacttgga ttgcttgatg atggaggtca tgtggtaaag tatctggatc
    20101 ctaaaataac tgcccggaac tgctgcaaac gatcttattt gagaggcttg tttatgtgca
    20161 aaggttttgt caaccggccg gaagccaatt accatttgga aatcgttttt aacgatacac
    20221 gtatggctaa agaagtaaaa aaactgatcg gcaggctcaa tttggaaacc ggcattggag
    20281 agcgtaaaaa taatctgatt gtatatatta aagacagtga gaaaatcgtt gattttttac
    20341 gcctgatcgg tgccagcaag gccttgttgg aattcgagaa tgtacgtata gtgaaatcaa
    20401 tgcgcaatca ggtgaaccga caagtcaatt gtgaaaccgc caatctcggc aaaaccattg
    20461 acgcctctgt tcgccaggtc gaattgatcg aagcgttgat tgaccaactg gggatgaaca
    20521 agattcctgt tcacttacgg gagttggctt tgttgcgcat tgaacatccc gacagtacgc
    20581 tcaaggaact cggcacactg atgagcccgc ctttgaccaa gtccggggtc gcctatcgga
    20641 tgagaaaatt ggaaatcttt gcagaagaaa atttatatgg accgtaaatg gcaggattta
    20701 atggatgata tatagaatat atttaaataa cattgtcata aaatgtcgtt caggataaat
    20761 ataaaatcca cattggcaac gataaagaga ggtatctcat gaagctaagt catgatattt
    20821 atttggatca acagcaaaaa ttattaatga ctccggaact tcgccaagcc attgccatgc
    20881 tgcagatgtc cactttggaa ctgggccaat atgtgcagaa cgaactggaa aacaatcctt
    20941 ttctggaaga aaaggatgct gatgatcatg aggaattgga aaccaatgct gaaaaactgg
    21001 atgaggttca agccgacaac aaatcagaag aatggttgga gtattataat gacagggata
    21061 ttggcctgac cgtagccgat gttgaagaaa agtcttttga tgcttttgtc agcaaatcac
    21121 ccagtcttta tgatcatctg cagtttcagc tgcatatgac catttgtgac gagagtgagc
    21181 tgatgatcgg cgaatatatt ctcggcagta ttgatgccaa cgggtatttg accgtgggac
    21241 tggaagaact ggtgcaagct accggtaccg atttggagag ggtccagcat atgctgacga
    21301 tcatccagtc ttttcatccg catggggttg gtgcccgtga tctggccgaa tgtctgctga
    21361 tacagctgga gcattacggc aaagatgatc caatcgcaac ggctattatt gaagagcacc
    21421 ttcaggatct gggcaaggga cgaatcaata aaatcgccca acagcttggg gtaggcgtcc
    21481 agcaggtgca ggaagcgtgt gattttattc gcagtctgga tcccaagccc gggctgcaat
    21541 acagcaacgg tgacgatacc aaatacatcg ttgcggatat tattttggaa aaggttgacg
    21601 gagaatacgt tgttattgtc aatgacagtg gctatcccca tctgatgatc aaccgactgt
    21661 atgaaaaaat gatacgcaat cctgaatcat tttctcccga agcacgcaaa tacatggaag
    21721 aaaaaatggg ctcagccgtg tggttaatta aaagcattga gcaacggcgt ttaacactct
    21781 acaaagtggc ccgctgcatc gtggatatcc aaaaggattt tctggatagg gggattaaat
    21841 acatcaaacc gcttaacctc aaacaggtag cggatctggt cgaagtacat gaatcaacgg
    21901 tcagccgcgc aacttccaac aaatatatcc agactccgca gggactgttc gaattaaaat
    21961 ttttctttgg cagcggaata agttcttatg ccaatgccaa ttgtgacaaa gtgtcttcca
    22021 agagcataaa gaaacagatt caggaactga ttgaaaagga agattctttt aatcctttaa
    22081 gtgaccagca aatcgtggaa atcatcaagc aacaaggagc agtcatctct agacggacgg
    22141 tggccaaata tcggcaggaa ctcggcatcc agtctaccgt tgcccgaaag agatattaaa
    22201 tagaaaaact taccgcgaat gtagcacatt aattatggaa tgtgctataa tatttccgca
    22261 accaaaattg accatgtttt gtagcctggc cgggacaaat atgtccctat agcttgaata
    22321 ttaccccgga gcatatgaat agctgaggat agcaatggat aaattttttg caattatgga
    22381 acgcttcgct cctgaagccg tggaaa
//
LOCUS       DAOTHZ010000035        20990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3030_length_20990_cov_7.649678, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000035 DAOTHZ010000000
VERSION     DAOTHZ010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 20990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20990
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3030_length_20990_cov_7.649678"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="pepT"
                     /locus_tag="P5549_05685"
     CDS             complement(<1..908)
                     /gene="pepT"
                     /locus_tag="P5549_05685"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF009920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRW12597.1"
                     /translation="MESLLERFLRYVKIDTQSSESSYTHPSTPGQMQLAKLLVAELQK
                     LSLTNINLSDNGYIMAELPANSNNTFPVIGFIAHLDTSPDVSGKDVKPMLFPDYDGQD
                     LVLNPAENTVMKVQDHPELKEYIGHTIITSNGHTLLGADDKAGVAAIMNALAILKSKP
                     EIKHGKIMVAFTPDEEIGQGADNFDVAGFGADFAYTVDGGPVGELEYETLNAAISQID
                     LQGINVHPGTAYMHMHNAILAAMELNSMLPVNQRPKYTRDREGFFHLTQMSGTVEKAK
                     MTYIIRDHDRNEFEQKKDYLQSCVDFL"
     assembly_gap    1334..1433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1434..3146
                     /locus_tag="P5549_05690"
     CDS             <1434..3146
                     /locus_tag="P5549_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRW12598.1"
                     /translation="TPPELSSCQNLLEGVKRIRKYMDRMQSIGPGIAAYAWSMYELEE
                     LNEEIDRCIINGRVDDRATPELARIRKRILIVEERIKQKLNDILRSAAYKGMLQDPIV
                     SSRGGRYVVPVRRQDMKKFPGQVLDMSSTGSTVFMEPAAVRQLQEELNLLQIEEINEV
                     SRILADLSGQVGDHHRELSINMEAMAHYDFLFAKARYSRSMNMHAVSLNRANQINILQ
                     GRHPLLGQSAVPLDFSIGWDYRALIITGPNTGGKTVALKTVGLLTLMVQCGLHVPAAA
                     GSEFAVFSDILADIGDGQSIEQSLSTFSSHVKNIQMIVKTCDASTLVIMDELGAGTEP
                     GEGRGFAIAVLEEVFARGAVIVATTHFGEIKEYALKTPGFENGCMAFDIASLRPLYQL
                     HIGNWGDSNAFLIALRLGMERHIIERAHELTYGERVSYAADQQKYVNNIVDDHEINAR
                     LSEKFEAQERRQVKEKRERSQRYEKKELEVGDRVYISTMQRTGVVCEEQNNRGDLVIM
                     VMGKKYVINHKRLTLQIDREDLYPENYDMDIIFETKEDRKKRKIMSKHHVEGLVIEHD
                     DGKK"
     gene            complement(3167..4078)
                     /locus_tag="P5549_05695"
     CDS             complement(3167..4078)
                     /locus_tag="P5549_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12599.1"
                     /translation="MVIDQIGHIKGNLYYLGFTECPIYLWDGPEPVIFDAGVTSAGKI
                     YVNAIRSVLGDRQPAMLCLTHCHWDHCGTAAYLKQAFPEMKIAATARGAGTIKRPNAL
                     ALIRMLNDATLKKIAAAPVDPSYLNTDPFAPFEIDIDLQDGQVISLADGTIIEVLATP
                     GHTQDHHSFWLPGEKILFAGEAAGVFYSHDTITTEFAYDYDAYLASLLRLAALPVEIF
                     CQGHYYILAGQEEVSTFFAKSIRETTGFKDRVMELLDEESGSVERVISRVKAERYDGN
                     LDLKQPEATYMLNLKAKVAHLAGKRQA"
     gene            4462..4656
                     /locus_tag="P5549_05700"
     CDS             4462..4656
                     /locus_tag="P5549_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRW12600.1"
                     /translation="MKKTVKELRKNQGLTAKELAARLKVDNVEILNIDELKLKDVPEP
                     LKSKLLPVLKGNYTDKIPWL"
     gene            4770..6089
                     /locus_tag="P5549_05705"
     CDS             4770..6089
                     /locus_tag="P5549_05705"
                     /inference="COORDINATES: protein motif:HMM:NF027438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HRW12601.1"
                     /translation="MKRKWLRVLLAVLVLWSGVSYYFYTQHKYVVQEYDLNKTVKVGE
                     TQVSFRQVCFTHYSNKTQQGYGYNLPWYYKAAGRLPAKMQLPLIKVISFYRSPYQVNP
                     DGTLKVKGIIISPQPYEENEGPLGKSLDIELYYGEDRFTLTGRQQEWGSNVCMFSING
                     NHVPEAVDEVTAVVTDKKTGEKKSLLIKPDWETKVYHNSWRPEFPFSPKETVRQLAMV
                     LSDRGIEQNKLQPFVLSAVVSEFPWENLQHSYWQQGYKTEMSYLGSYKDFQDVYSVQL
                     MFLAGTDEIVATDKIVASQKFYLVEQRDGWKLIDISKTGSESNSVVADQFATAQQIVE
                     SQGYSIMMNSGANFDFRLPVSFNEINDDVKIGEFLKDRNELSKQNGFDFSSYLGQEVT
                     LVTYGVINEKNVEANVDLILHGNKIVGFWVENQGEPSDFGVIVNAYQ"
     gene            6132..6473
                     /locus_tag="P5549_05710"
     CDS             6132..6473
                     /locus_tag="P5549_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12602.1"
                     /translation="MPLPLAIICGILLAVLIYGGGVRLILLSILLLPIYYAIKNAWME
                     LPKDGKWKVNWERFFKVIFVELIVGIILAVAQSEQLASISWLIMAYFLYMLSTSLFIS
                     MTKDIQKHLLL"
     gene            6700..7557
                     /locus_tag="P5549_05715"
     CDS             6700..7557
                     /locus_tag="P5549_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRW12603.1"
                     /translation="MVTREPLHKRVKIKDSLVMEYLLYDGPGPTILFLHGTGFSPWLW
                     HPIARRLNDEFRIIAPFSCDHRDAEPEEGGLKWKLLAEDLVAFCHQLNLSNLYIVAHS
                     MGATVATITASLDETLARKMLLMEPIYLPEALYSMKMSVDQHPLASKSIKRRNAWRDR
                     QEAREYLLARALYNSWDAEMLDLFLCHGMVENEAGGIRLACSPRREAAVFMGGVHYNP
                     WPLLREIQCPVLVMEGECSENRPLIDLKRAASLIPQGEYRLITGIGHTIPMEIPEKIT
                     ALTRHFCHS"
     gene            7725..8543
                     /gene="speD"
                     /locus_tag="P5549_05720"
     CDS             7725..8543
                     /gene="speD"
                     /locus_tag="P5549_05720"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRW12604.1"
                     /translation="MEIKPLNKKLRLFGFNNLTKTLSFNLYDICYALKPEQSHDYIEY
                     IDEEYNARRLTEILTNVAKIIDATILNIARQDYDPQGASVTILIAESDVHPGATCGET
                     VAETPGPLPEAVVGHLDKSHITVHTYPETHPDQGISTFRADIDISTCGHISPLKALNY
                     LIHTFQPDMCFIDYRVRGFTRDMDGKKFFIDHKINSIQNYITSKTRNQYQMIDVNVYQ
                     ENIFHTKMLLKEFDLDNYLFGSSKKELKSKDKVRIKKQIKHEMMEIYSGRNIPK"
     gene            complement(8608..8814)
                     /gene="copZ"
                     /locus_tag="P5549_05725"
     CDS             complement(8608..8814)
                     /gene="copZ"
                     /locus_tag="P5549_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HRW12605.1"
                     /translation="MASSVLEVTGMSCSHCEHSVKTAAGSLAGVKEVKVELKTGQVTV
                     EYDPAQVKLQAIREAIEDQGYDVK"
     gene            complement(8867..11287)
                     /locus_tag="P5549_05730"
     CDS             complement(8867..11287)
                     /locus_tag="P5549_05730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRW12606.1"
                     /translation="MERRESLKISGMTCAACASRIEKGLTKIEGVKQANVNLALEKAT
                     VEFDDKLTDPQQLAATIKRLGYHVIDDQADANSRVVLNLYGMSCAACASRIEKKLGAT
                     EGVTHAAVNLSTEKAVVDYDKTKIKLSDMLQIIEKLGYRAERSEEISRDREKEAREKE
                     IRSLKTELIVAAVLSSPLLLAMILSLFRLDVPSLHNQYYQLALATPVQFIIGWRFYKN
                     SYYALRSGSANMDVLIAMGTSAAYFYSLYNVFFQTVPPGIMKDLYFEASAVVITLILL
                     GKYLEAAAKGKTSEAIKKLMGLSPKTARVVRGGQEMDIPIEEVKIGDLIIVRPGEKVP
                     VDGVITAGSSSLDESMLTGESLPVEKSIGDQVVGATINKYGTFKFEATKIGQDTVLAQ
                     IIKLVEDAQGFKAPIQKMADQVAGVFVPAVITVALITFAVWFLTSADVSKSIVSAVAV
                     LVIACPCALGLATPTAIMVGTGLGAENGILIKSGEHLESAGKINVIVLDKTGTITKGT
                     PDVTDIIAVGNLEMEEILRLAAIAEKKSEHPLAAAIYQFALAHLEQVADPEQFEALPG
                     KGIRAVVQGKEIIIGTRQLMWEQQIDLAQCEIQLARLEDDGKTAMLMAVEASLQAIIA
                     VADTLKEHSREAIEEFRQMGLEVFMITGDNRRTAQAIAAQAGIRQVLAEVLPENKAEE
                     IKKLQQTGKIVAMVGDGINDAPALAIADIGMAVGTGTDVAMEAADITLMRGDLRAVSA
                     AIRLSRQTMRKIKQNLFWAFIYNIIGIPFAAFGLLNPVIASAAMAFSSVSVVTNSLSL
                     KRFDPRSQ"
     gene            complement(11354..12577)
                     /locus_tag="P5549_05735"
     CDS             complement(11354..12577)
                     /locus_tag="P5549_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12607.1"
                     /translation="MDKIMLPGQFNPGGIRKRLLLLALLMLCSMGLCIGCVNNKDSQP
                     ANSILYENHTLGFSLKLPLNWENNYMAREADHQVAFYSKKVNEKSGGGALFTIVRMTG
                     ELITPEDMQQAPVGQQIILQGNGFTYFARMPSDVQYPPNDKALSSDYKTMAEQINNIL
                     DSAALLGNEKPKPANEGYKVVGSSFFTVEIPDNWELRAADQVPPDWPISIAGKSIGWI
                     ALIPYKSELLEDSDDDNGMRAYLFDDESFREVRITLNKEYADQATMEKIKNSFKFAGG
                     PFNVVDLQTNAEKYLAGGGKKVFGKIDGFEMKNGKPAAVRVSIMKFITDTSGDKYPNG
                     FGIEDLHKLETYPLDLGVTVAPLVTPHHNSLGFYAMPLLDENFIKNYDYKNYYYDFIF
                     GPDGQLKILLGHYIP"
     gene            13034..13789
                     /locus_tag="P5549_05740"
     CDS             13034..13789
                     /locus_tag="P5549_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRW12608.1"
                     /translation="MLDEFSRTQLLLGKDAMTKLSASHVAVFGIGGVGSYAVEGLARS
                     GVGKLTLVDHDVVDLSNLNRQIHATHKTIGQPKVEVMRQRILDINPRAQINNYQHFYL
                     PDQAEDILNADYDYIIDAVDTVTAKIDLIVQAQQRGIPVISCMGTGNKLDPGRLQIDD
                     IYSTSVCPLAKVMRRELRRRGITALQVVYSREEPVKPQVEEDNGSENECESCLKSGIC
                     SGKRNLPGSITFVPAVAGLLLASQVVRDLIAGM"
     gene            13955..14884
                     /gene="cysK"
                     /locus_tag="P5549_05745"
     CDS             13955..14884
                     /gene="cysK"
                     /locus_tag="P5549_05745"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRW12609.1"
                     /translation="MAKIATQITDLIGNTPLLELGAYSREMDLQARLIAKLEYFNPAG
                     SVKDRVGYAMIKDAEEKGLLKPGSVIIEPTSGNTGVGLTFVAVAKGYRIIIVMPDSMS
                     MERRILMKALGAELVLTPGAEGMPGAIKRAEELALEIEDSFIPQQFENPSNPEAHRQT
                     TVQEILHDTDGQVDFFVAGVGTGGTITGIGEVLKRYNPQIKIVAVEPFDSPVLAGGKA
                     GPHGLQGIGAGIIPKVLNVNILDEIIPVKTEEAYATVHRLVKTEGLLVGISSGAAVFA
                     AAQIAKRAENKGKQIVVLLPDSGERYLSTPLFA"
     gene            15038..15841
                     /gene="larE"
                     /locus_tag="P5549_05750"
     CDS             15038..15841
                     /gene="larE"
                     /locus_tag="P5549_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRW12610.1"
                     /translation="MDLNEKLGQLIYNLGSMSGLAVGFSGGVDSSLLLKVAHGVMGRN
                     VLAVIVDTPTMPRRELQDALDFVKQAQVNWQVIPADHLQIEGFAANPANRCYLCKREM
                     FGRIKKVAQESHLEWIADGSNLNDLAEDRPGMQALRELGIISPLLEADLTKEEIRQLA
                     RRMQLKTWDKHANSCLATRFACGQEISLPKLRQVEEAEAYLNDCGFRQIRVRSYDHAA
                     RIEVSPEERAKFGDLQLMDAVHQKLKELGFVHVSLDLQGYRQVKAEKQP"
     gene            complement(15918..16850)
                     /locus_tag="P5549_05755"
     CDS             complement(15918..16850)
                     /locus_tag="P5549_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRW12611.1"
                     /translation="MSRLNPQQTSQLVAAIVNSYRENQTAVSMESVSQPRREVIVKVI
                     FMLRELLFPEYFGQRQLISTTIEYHIGDLLINLHRILHEQIVLALTHQAISKDIPLDD
                     VEAQASEIIYRFLDKIPALRKSIELDIQAAFDGDPAATSKDEVMFSYPGVFAVSVYRM
                     AHELQLLAVPLIPRIMTEYAHSVTGIDIHPGAAIGKYFFIDHGTGVVIGETTSIGNNV
                     KVYQGVTLGALSTREGQNLRGVKRHPTIEDDVTVYSGTSILGGETVIGKGVVIGSNVF
                     LTRSVPAGTKVSLKAPDLVFKGQSGPGADSDLDI"
     gene            complement(17025..18857)
                     /locus_tag="P5549_05760"
     CDS             complement(17025..18857)
                     /locus_tag="P5549_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12612.1"
                     /translation="MSSNFINTTRDHKFILKEWLDLTKVLETERFQGGYSVDDIDFIL
                     DNALKGAKEVVAPTNEDADKIGGIYVDGHARMTESCKTAYHFIQQNGFCSSNKDRNDP
                     SAVPLSVMWAMMEFMIGANPHIGTILAGPAAGGEVLEEFGSEYLINMYAGRMFSGEWT
                     ATMCLTEPSAGSDVGDQTTKAYPTDETGVYMIKGTKCFISHAENGICDNTIHLVLARI
                     EGAAPGTKGLSLFVIPQYFSEMDGTILGPNDVSVSGIEHKMGIKGNVTCVLNFGENGQ
                     CKGWLVGDPPGEDGVGKGMMQMFRVMNEERLMTGHGALSLSAVAYHNAVAYCKERIQG
                     RPFTNPKGNRVPIIEHEDIRRMLLFQKSHVEVFRALILYNFYLLDIAYNSSDKELAKQ
                     CDALVSVNIPLIKAYCSDMAIECISNAMQCYGGYGFSEEYPIAELYRDCRIYPIWEGT
                     NFIQSLDLVGRKFTMAKGQVFGLWLSQISDFLNANRGAAGFEKEFAIMDAALLSYQAI
                     LGTLGGYMGEGKVRMMPLFSTRILHATAKLLGGKLILDQALLAQKKVDELGPDHFDYA
                     FYAGKIASARFYIGNIVPEIETVSQIFKNGDKSALEIPEEAFFV"
     gene            complement(19132..19542)
                     /locus_tag="P5549_05765"
     CDS             complement(19132..19542)
                     /locus_tag="P5549_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12613.1"
                     /translation="MKKPVYFINKFHKGNFLRLIKKAGKNYPDITPIAYVLAAIGTLG
                     NSEGSFAIIDKYFLPTDIDFESLSQTLTGDAADRALLKVAANLYNSENYANLSDVFSP
                     LNDEYQAVIYQALLVKFPSFSRSWDKKYPYAEGD"
     gene            19746..20195
                     /locus_tag="P5549_05770"
     CDS             19746..20195
                     /locus_tag="P5549_05770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRW12614.1"
                     /translation="MKDFSQSEQEPEEKLNIKLGVAPNCFVFMQGRMIDFNGNERLTI
                     AFPVLESYLNPGGSMQGGFITAAFDNVFGPLCQAVTGTKATTTVDLYTNYHRPIFAGD
                     ELVITASIIVKGKTKIHMTGAAYNQANQLIATASTNYIYLKAPRSGA"
     gene            20399..>20990
                     /locus_tag="P5549_05775"
     CDS             20399..>20990
                     /locus_tag="P5549_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRW12615.1"
                     /translation="MPAIYMDNAATTYPKPDSVYRAVDHFNRNMGGNPGRGSSQQSIE
                     AGSVILQTREALARFFNIKDSLDIAFTANVTESLNIGLKGLLRHGDHVITSSMEHNAV
                     ARPLYALRRHGIEWTQVACAADGSLDPQDIARAIKPNTRMICTLHASNVTGTILPIRE
                     IGRLAREAGIIYMVDSAQSAGVLPIDVQADAIDILAF"
BASE COUNT         5408 a         4732 c         5225 g         5525 t
ORIGIN      
        1 agaaaatcca cacaggattg taaatagtct ttcttctgct caaattcatt ccgatcatga
       61 tctctgatga tgtaggtcat ttttgctttt tcaaccgttc cgctcatctg ggtcagatga
      121 aaaaagcctt cgcgatcacg ggtatatttg ggacgttgat tgaccggcag catgctgttc
      181 aattccatgg cggccaggat ggcgttgtgc atgtgcatgt aggcggtacc gggatgtaca
      241 ttgattccct gaagatcaat ctgggaaata gctgcgttta aggtctcgta ttcaagctct
      301 cccaccggcc ctccgtccac ggtataggca aagtcggcgc caaatccggc tacgtcaaag
      361 ttgtctgccc cctgtccgat ctcttcatcc ggggtaaaag cgaccatgat cttgccgtgt
      421 tttatttccg gtttgctttt caaaatggcc agggcattca tgatcgcggc gactccggcc
      481 ttgtcatctg ctcccagcag cgtatgtcca ttgctggtta taatggtatg tccgatatat
      541 tcttttaatt caggatgatc ctgaactttc atcaccgtat tttcagccgg gttcaggacc
      601 aaatcctgac catcataatc tggaaacaac atcggtttga catccttgcc ggatacatcc
      661 gggctggtat ccagatgggc gatgaatcca atgacgggga aagtattgtt gctgttggca
      721 ggcagttcag ccattatgta accattgtca cttaggttta tattggtcaa actcagtttc
      781 tgaagctcag cgaccagcaa ttttgccagc tgcatttgtc cgggcgtgct ggggtgggta
      841 taagagctct ctgaagattg cgtatcaatt ttgacatatc gtaagaatct ttccaaaagg
      901 ctttccatca atattcctcc catagaacga tttattgcta gactataatt gtataatttg
      961 gcaggcgtaa tatcaaattg acgggagatt atctttattg ttgaagttgt gggttttcaa
     1021 taaaattaaa tttctctcca ggaatatcct tgagaagttt aattttacat atgaaggaac
     1081 gttgaaaatg gcggagaagc tatttaacgg caatatttat gatcatgact gctacttttt
     1141 gaccgcccgg agataatact tgtcacccag tcatttcttc cttgactttc tttgccaggc
     1201 gcgttatttt tataatgtct tatgacaatt ttacagtaag gaatgaatgt atgtccgggg
     1261 tagttcataa ataaccataa tttgatatgg gccttgattc gtagttggtt gtaccgttcg
     1321 atatggtact ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaccccgc
     1441 cggaactgag ttcctgtcag aacctgctgg aaggagtgaa gcggatcaga aaatacatgg
     1501 atcgcatgca gtccattggc ccggggattg ccgcttatgc ctggtccatg tatgaactgg
     1561 aagagctgaa tgaggagatc gatcgctgta tcatcaatgg ccgggtggat gaccgggcga
     1621 cgccggagct ggcgaggatt cgcaagcgga ttttaatcgt cgaggagcgg attaaacaaa
     1681 aactgaatga cattcttcgt tccgcagcct acaagggcat gctgcaggat cccattgtca
     1741 gcagccgggg cggtcgttat gtggtgccgg tgcgccgcca ggatatgaaa aaatttcctg
     1801 gtcaggtgct cgatatgtcc tccaccgggt caaccgtttt catggagcca gccgcagtca
     1861 ggcaattgca ggaggaatta aatctgctgc aaatcgagga gattaatgaa gtcagccgca
     1921 tattggccga tctgtccggg caggtgggcg atcatcatcg tgagctttcc atcaatatgg
     1981 aggccatggc ccattatgat ttcctgtttg ccaaggccag atacagtcgc agtatgaaca
     2041 tgcatgcagt cagcctgaat cgggccaatc aaatcaacat tctacagggg cgtcatccat
     2101 tattggggca gtctgctgtt ccgctggatt tcagtatcgg ctgggactat cgggcgctga
     2161 tcattaccgg ccccaatacc ggcggaaaaa cagtggcttt gaagacggta ggattgctga
     2221 ccttgatggt gcagtgcggt ttgcatgtgc cggccgccgc cggcagtgag ttcgcggttt
     2281 tttctgacat tctggctgat attggagacg ggcagagtat tgagcaatcc ctgagtacgt
     2341 tttcgtctca tgtgaaaaat attcaaatga tcgtcaagac ttgtgacgcc tccactctgg
     2401 tcattatgga tgaactggga gcggggacgg aacccggcga gggccggggg tttgcgattg
     2461 cggtgctgga ggaggtattt gcacggggcg cggtgattgt agccacgacc cactttggtg
     2521 agattaagga atatgccctc aaaacaccgg gttttgagaa tggctgcatg gcttttgata
     2581 ttgccagcct gcgtccgctt tatcagctgc atatcggcaa ttggggggac agcaatgcgt
     2641 tcctcatcgc cctgcggctg ggaatggaac ggcacattat tgagcgcgcc catgagctca
     2701 cctacggaga aagagtcagc tacgccgccg atcagcaaaa atacgtgaac aacatcgtgg
     2761 acgatcatga gatcaatgcc cgcctgagtg aaaaatttga agcgcaagaa cgccgccagg
     2821 tcaaggagaa gcgcgagcgc agccaacgct atgagaagaa agaacttgag gtgggagacc
     2881 gggtctacat cagcaccatg cagcggaccg gcgtcgtatg tgaggagcag aataaccggg
     2941 gcgatctggt gataatggtg atgggtaaaa aatatgtgat caaccataaa cgacttaccc
     3001 tgcaaataga ccgcgaagac ctgtatccgg aaaattacga tatggatatc atctttgaaa
     3061 ccaaggaaga ccgtaaaaaa cgcaaaatca tgagcaaaca tcacgtggag ggtctggtta
     3121 tcgaacacga tgatgggaaa aaataaagca ctgggggagt aacgctctat gcctgtcgct
     3181 tgccggccag gtgggcgact ttcgccttga ggttcaacat ataggtggct tccggttgct
     3241 tcagatcgag gttgccgtca tagcgttccg ctttgacccg gctgatgact ctttccaccg
     3301 agccgctttc ttcgtccagc agttccataa cccggtcttt gaaaccggtg gtttcgcgaa
     3361 tcgatttggc aaaaaaagtg ctgacttcct cttgaccggc caggatataa tagtgtccct
     3421 gacaaaagat ttccaccggg agtgctgcca gccgcagcag ggaagccaga taagcatcat
     3481 aatcataggc gaattcagtg gtgatcgtgt cgtggctgta gaaaaccccg gccgcttctc
     3541 cggcaaagag gatcttctcc ccaggcagcc agaaactgtg gtgatcctgg gtatggccgg
     3601 gcgtggccag gacctcaatg atggtgccgt cggccagcga aatgacttga ccgtcctgca
     3661 gatcgatatc gatttcaaac ggcgcaaaag ggtcggtatt caaataggat ggatcaaccg
     3721 gagcagcggc aatctttttt agggtggcat cattcagcat gcggatcaga gccagggcat
     3781 tggggcgctt gatcgtgcct gcccctcgag cggtggcggc gattttcatt tccggaaagg
     3841 cttgcttgag ataggcggca gtgccgcagt gatcccaatg gcaatgggtt aggcacagca
     3901 ttgccggctg ccggtcaccg agcacggaac ggatggcgtt gacatagatt tttccggcgc
     3961 tggtcacgcc cgcatcaaat atcaccggtt ccggcccgtc ccacagatag atcggacatt
     4021 ccgtgaagcc cagatagtaa agattgcctt taatatgccc gatttgatcg atcaccatgt
     4081 ttgtacctcc cccggctgcc tgcttctcac tccgtctgca gaacattttt cttctggttc
     4141 catttattat aacagatcac agcattatcg tatgggactt cctgtcggac tctttatagg
     4201 ctgtttcggc aaaccgggtt gtttcgtgtt ttctcccggt tgactttttc aaccaaaatc
     4261 aactgcaaat ccatcgtcag aatcaaaagc agtggataca ataaaagcaa ggaggggatc
     4321 acacatgaaa aattttaaga taggcaggat cattatgatt aaacaaggaa cttaaaataa
     4381 taatcatcag cgaggtgaaa tagtatgtaa gcagcggctg atacgcaacg gaggtgggta
     4441 gtgttttttc aatcaccgat tatgaagaaa accgtaaagg aattgcgcaa gaaccaaggc
     4501 ctcaccgcca aagaattggc tgccaggctc aaggttgaca acgttgagat attaaatata
     4561 gacgagttaa agctcaaaga tgtgccggaa ccgctcaagt cgaaactcct gccggtatta
     4621 aagggcaact acacggacaa aatcccctgg ctttaacatc gaaatttgtc gataagtttt
     4681 tctgttatga gcagggtcag gggccaatat taccgtaata ttgaatatac gatggcataa
     4741 atcaatgtcg ggttggggag gaatatgtaa tgaaaagaaa atggctgcgt gtgttgcttg
     4801 ctgtcttggt tctgtggtca ggggttagtt attattttta tactcagcac aagtatgtgg
     4861 ttcaggaata tgacctcaat aaaacggtga aagtagggga gacgcaggtt agctttaggc
     4921 aggtttgctt tacccattac agcaataaaa cacaacaggg atatgggtat aaccttccct
     4981 ggtattataa agcagctggc cgtttgccgg ccaagatgca gctgccgttg atcaaggtta
     5041 tcagtttcta tagaagtccc tatcaagtaa atcctgacgg cacgctcaaa gtaaaaggga
     5101 tcattatttc tccgcagcca tacgaggaaa atgaggggcc tttgggcaaa agtctagaca
     5161 tagaattgta ttacggagaa gacagattta cactaaccgg tcgacagcag gagtggggct
     5221 ccaacgtttg catgttttcc attaatggta atcacgtacc cgaggcagtt gatgaagtaa
     5281 cggcagtggt tacggataaa aagacaggag agaaaaaaag tctgctcatc aaacctgatt
     5341 gggaaactaa agtttatcat aattcatggc ggcctgaatt tccatttagt ccaaaggaaa
     5401 ccgtaagaca gttggcaatg gtcctttcag acaggggcat tgagcaaaat aaattacaac
     5461 cttttgtttt gagtgcggtt gtgagtgagt ttccctggga aaatctacag cactcgtatt
     5521 ggcagcaggg ttataagact gaaatgtctt acctgggaag ctataaagac tttcaggatg
     5581 tatattctgt tcaactgatg tttttagctg gaactgatga aatcgtggca actgacaaaa
     5641 ttgtagccag ccaaaaattt tacttggttg aacaaaggga tggttggaag ttaatcgata
     5701 tttccaaaac cgggagtgaa agtaattcag ttgtggcaga tcaatttgca acggcccaac
     5761 aaattgtgga aagtcagggt tattctatca tgatgaatag cggagcaaac tttgattttc
     5821 ggcttccagt ttcctttaat gagataaacg atgacgttaa gattggtgag tttttaaagg
     5881 acaggaatga attatcaaag caaaatggat ttgatttttc cagttattta gggcaagaag
     5941 ttacgcttgt tacctacggt gttataaatg aaaagaatgt tgaagcaaat gttgatctta
     6001 tattgcatgg aaataaaata gtgggattct gggttgaaaa tcagggagaa ccctccgatt
     6061 ttggcgtgat cgtgaacgcc tatcaatagg tggcgtctga gacaaaaaat tatcaataaa
     6121 tggaggttaa tatgccatta ccgttagcta taatatgcgg tatcctcctc gccgtactta
     6181 tatatggggg aggagtacgt ttaatactac taagcatttt actgcttcct atttattatg
     6241 ccatcaagaa tgcatggatg gaattgccca aagacggaaa atggaaagtg aactgggaaa
     6301 gattttttaa ggtgattttt gtagagctga ttgtaggtat aatattggcg gttgcgcaaa
     6361 gtgaacaact ggcttcgatt tcttggttga ttatggcata ttttttgtac atgctatcca
     6421 catccttatt catctccatg accaaagaca ttcaaaaaca tttattgcta taatcttggc
     6481 atttgtaatt aatcctgacc gcctatcagg caagattaca atgagattga aatatttatt
     6541 gccagatgac aaggacaagg agatctcttt gaagaagtat tatctgggag aatgggaacg
     6601 cagcaggttt attggagagt aataaagcgt caacaagttg ggtgtgaacc cttgatgtgt
     6661 tggggtgagg ttgcgctttt gaggggagca tggcgaaaga tggtgactcg tgagcctttg
     6721 cataaacggg tgaagatcaa ggattcgttg gttatggagt atttgcttta tgatggaccg
     6781 gggccgacca tcctgttcct gcatggaacc ggtttttcgc cttggctgtg gcatccgatc
     6841 gctcgtcggc ttaacgatga gttcaggatt attgcccctt tttcttgcga tcaccgggat
     6901 gctgaaccgg aggaaggcgg attgaagtgg aagctgctgg ctgaagatct ggttgcattc
     6961 tgtcatcaac tcaatctgag caacctttac atagtggctc attccatggg ggcaaccgta
     7021 gccaccataa cggcttcgct tgatgaaact ctggcccgca agatgctcct aatggaaccg
     7081 atctatctgc ccgaagctct ttattcgatg aagatgagtg ttgatcagca tccccttgcc
     7141 tccaaatcca taaagcggcg caatgcctgg cgggaccggc aggaggcgcg ggaatatctg
     7201 ctggctagag ccctttataa cagctgggat gcggagatgc tggacctgtt cctttgccat
     7261 ggtatggtgg agaatgaggc cgggggcatc cgcctggcct gttcgccgag acgtgaggca
     7321 gctgttttta tgggcggtgt acattataat ccctggccgc tcttgcgcga gatacaatgc
     7381 ccggtgctgg taatggaggg cgaatgcagt gaaaaccggc cgttgattga tctcaagagg
     7441 gcggcctctc tgatccctca aggagaatat cgtttaatta caggcatcgg tcacaccatc
     7501 ccgatggaaa ttccggaaaa gatcactgcg ttgaccagac atttctgcca ttcataaaga
     7561 aagcagtcaa aacttttatt cccagacgga aacaactcat tattcatcca gtggacactg
     7621 ccaaatattg aaaacgcgat catcgttgtt tggctattga atttgttccg gtaatccatt
     7681 atcttttaat aaacattaat gcattcatca cggagggatc gaatttggaa atcaaaccac
     7741 ttaacaagaa gctcaggcta tttggtttca acaatttgac caaaaccttg agctttaatc
     7801 tttatgatat ctgctacgct ctcaaaccgg aacaaagtca tgattatatt gaatatatag
     7861 atgaagagta taacgcccga cggctgactg agattctgac caacgtggcc aaaatcattg
     7921 atgctaccat ccttaatatt gcccggcagg actatgatcc ccaaggtgcc agtgtcacca
     7981 ttctgatagc tgagagcgat gtgcatccgg gagcaacctg tggggaaaca gttgctgaaa
     8041 cgccgggacc actgccggag gcggttgtgg gtcatctgga caagagccat atcacggtgc
     8101 atacctatcc ggaaacccat cctgatcagg gaatcagcac ttttcgggcg gatatcgata
     8161 tttctacctg tggtcatatc tctccgctca aggctttgaa ttatctgatt catacttttc
     8221 agccggatat gtgttttatt gattatcggg tgcggggctt cacccgtgat atggacggga
     8281 aaaagttctt tattgatcat aaaattaatt ccatccaaaa ttacattacc agcaagaccc
     8341 gcaatcaata tcaaatgatt gacgtgaacg tatatcagga aaatattttc cacaccaaaa
     8401 tgctgctcaa ggaattcgat ctggataatt atctgttcgg ttccagcaaa aaggaactga
     8461 aaagcaagga taaggtgaga atcaagaagc agatcaaaca tgagatgatg gaaatttact
     8521 ccggacgtaa tattcctaaa tagtgaaata aaaattcggg ctcatgtaat tgaacccgaa
     8581 ttttttataa tcgtatccgc aaactggtta tttcacgtca tatccctgat cttcgatggc
     8641 ctcccggatc gcctgcagtt tcacctgggc cgggtcatat tcaaccgtta cctggccggt
     8701 tttaagttcc accttgactt ctttcacgcc tgccagagaa cctgctgccg tcttcacact
     8761 gtgttcacag tgactgcagg acatgccggt tacctccaat actgaacttg ccatgatatt
     8821 tccgcctcct tttcgttgtt gctgatgatt atactgatcc agcggtttat tgcgatcggg
     8881 gatcaaatct ttttaaactg agggaattag tgaccaccga taccgaactg aaggccatag
     8941 ccgcactagc gatgaccggg ttcagcaacc caaacgcggc aaaggggatg ccaataatgt
     9001 tgtagataaa tgcccagaaa agattctgtt taatcttgcg catggtctgg cgggagagtc
     9061 tgatggcggc ggatacagcc cgcaaatctc cccgcatcag ggtgatgtca gccgcttcca
     9121 tggcaacatc cgtgccagtt cccacggcca tgccaatatc tgcgatggcc agggccggcg
     9181 cgtcattgat gccgtcgcct accatggcta caattttgcc ggtttgctga agttttttga
     9241 tttcctcggc cttgttttcc ggcaacacct cagccaatac ctggcggatg cctgcctgcg
     9301 cggcaatcgc ctgtgcggtt cgccggttgt caccagttat cataaagact tccaggccca
     9361 tttgccggaa ttcctcaatc gcttcccggg aatgctcctt gagcgtgtcg gcgacagcga
     9421 tgatggcctg caatgaagcc tccacagcca ttaacatagc ggtcttgcca tcgtcttcca
     9481 ggcgggcaag ctgtatttcg cattgcgcaa ggtcaatttg ctgctcccac atcagttgac
     9541 gggtgccaat tataatttcc ttgccctgca cgaccgcccg gatgcccttg ccgggcaggg
     9601 cctcgaactg ctccggatca gccacctgct caagatgggc cagagcaaat tgataaatcg
     9661 cggctgccag aggatgctcg gatttttttt ccgctatggc ggccaggcgc agaatttctt
     9721 ccatttccaa attaccgacg gcaatgatgt cagttacgtc cggggtacct ttggtaatgg
     9781 taccagtctt gtccagcacg atcacattga ttttaccggc tgattccaaa tgttcgccgc
     9841 ttttgataag gatgccgttt tccgctccta atccggttcc caccataatc gcggtgggag
     9901 tagccaaacc aagggcgcag gggcaggcaa tgaccagcac cgccacagcg ctgactatag
     9961 acttgctcac atctgcgctg gtcaaaaacc agacagcaaa ggttatcaag gccaccgtga
    10021 tgactgccgg cacgaatacc cccgcgacct gatcagccat tttttggatt ggtgccttga
    10081 agccctgggc atcttcaacc agctttatga tttgggccag aaccgtatct tggccaattt
    10141 tggtggcttc aaatttgaat gtgccatact tgttgatggt agccccgaca acctgatcac
    10201 cgatactctt ttccaccggc agactctcac cggtcagcat ggattcatcc agggaggaac
    10261 tgcctgcggt tatcaccccg tccaccggta ctttttcacc cggacggaca atgatcagat
    10321 cgccgatctt cacttcttcg atgggaatgt ccatttcctg accgcctcgt accacgcggg
    10381 cagttttcgg actgagcccc atcagtttct tgattgcttc cgaggttttg cccttggcgg
    10441 ctgcctccag atatttgccc agcaggatca gggtaatgac gaccgcactg gcctcaaaat
    10501 acaaatcttt catgatgccg ggcgggaccg tctggaaaaa aacattatac aaactgtaaa
    10561 aataggctgc agaggtgccc atggcgatca ggacgtccat gttggcacta ccggagcgca
    10621 gcgcataata ggaatttttg taaaacctcc agccgataat aaactgtacc ggagtagcca
    10681 gcgccagttg gtaatactga ttgtgcaggc tgggtacgtc gaggcggaaa agtgacagga
    10741 tcatagccag gagcaagggc gaactaagta ctgcggctac gatcagctct gttttgaggc
    10801 ttctgatttc cttttcccgg gcttcttttt cccggtcacg gctgatctct tcactgcgtt
    10861 ccgccctata gcccagtttt tcaattattt gcagcatatc gctgagctta attttggttt
    10921 tgtcataatc aaccacggct ttttccgtgc tcaggtttac tgctgcatgg gtcacaccct
    10981 cagttgcacc cagctttttt tcaattctgg acgcgcaggc ggcacaggac atgccgtata
    11041 aatttaaaac cacccggctg ttcgcatccg cctgatcatc aatgacgtga taacccagcc
    11101 ttttaatggt cgcagccaat tgttgggggt cagtcagttt gtcatcaaat tccacggtgg
    11161 ctttttccag ggccagatta acgttggcct gtttcacgcc ttctattttg gtcagacctt
    11221 tttcgatccg actggcgcag gctgcgcagg tcataccgct tatttttagt gattcccgtc
    11281 gttccataag atcactccta tggattatat tatatatatt ttatgcttct taatatacgt
    11341 tacacatgta atattaaggt atatagtgtc ctaataatat ttttaactgc ccgtccgggc
    11401 caaaaatgaa atcataataa taatttttat aatcgtagtt tttgataaaa ttttcatcca
    11461 gcaggggcat tgcataaaac cctaatgaat tgtgatgggg ggtaaccagg ggggcaacgg
    11521 ttacacctaa atccagggga taagtctcca gcttatgcaa atcttctatg ccaaagccgt
    11581 tcgggtattt atccccggat gtatcggtta taaatttcat tatgctaacc cgcacggcgg
    11641 ccggcttgcc atttttcatc tcaaaaccat ctatcttgcc gaatactttt ttgcctcctc
    11701 cggccagata tttttcagca ttggtttgta aatccactac attgaagggg ccgcccgcaa
    11761 acttaaaact gtttttgatt ttttccatgg tggcctgatc ggcatattct ttattaagcg
    11821 ttatgcgcac ttcccgaaaa gattcatcgt caaacagata ggcccgcatg ccattgtcgt
    11881 cgtcggaatc ctcaagcagt tcacttttat aaggaatcaa ggcaatccag ccgattgatt
    11941 ttcctgcaat cgaaataggc cagtcaggcg ggacttgatc agccgccctt aattcccagt
    12001 tgtcggggat ctcgacggta aagaagctgg atccgacaac tttgtaacct tcattggctg
    12061 gtttaggttt ttcattgccc agcagggcag cactatccaa tatattgttg atctgttctg
    12121 ccatcgtttt atagtcgctg ctcagagctt tatcattggg ggggtactgc acatcagacg
    12181 gcattctggc aaaatacgta aagccattgc cctgcaatat gatttgctga ccaacgggcg
    12241 cctgctgcat atcttccggc gtaatcagtt ccccggtcat ccttacaatg gtaaaaagag
    12301 ctcctccgcc tgatttttca tttacctttt tgctgtaaaa agccacctga tggtctgctt
    12361 ccctggccat gtagttattt tcccaattca gaggcaattt taatgagaac cccagcgtat
    12421 ggttttcata aagaatacta tttgctggtt gggaatcctt gttgttaaca cagccaatac
    12481 ataatcccat gctgcaaagc attaacaaag ccaataataa caggcgcttc ctgatcccgc
    12541 ccggattaaa ttggccgggc aacataatct tgtccattct gttttccccc ccctaaaatt
    12601 tatcaatatg cctaagatgg acctgaatcc tcataaaagc aattcaagag ccatctcgtt
    12661 aactatacga gacgaaaatg ggattgtttc acttgaaata ttaattttcg ttattgagtt
    12721 ttaagtttaa gtatcctgtg acttataatc ggttaaaaat gtagaacctg ctgaaaaaca
    12781 tgctatggtt taagcaattg ttttgatata tgaagaaaaa atcagtacaa tacaagctaa
    12841 gtacaaacaa tgattagggt catagacctt tgttgggtag tttttttgaa aactgttggc
    12901 taattaactt tggttaaaat caggttagaa atatttgaag tgataacaag ataaaggcta
    12961 taggatgatt ttaagtatgc acggcgctat atttttgtat tatgccaaat tcttattttg
    13021 aagaaggttt gccatgttag atgaattttc ccgtacacaa ctccttcttg gcaaggatgc
    13081 catgaccaaa ctatccgcca gccatgtggc cgtctttggc attggcgggg taggcagtta
    13141 tgcggtcgaa ggtctggccc ggtccggcgt tggcaagctg acgctggtgg atcatgatgt
    13201 cgtagatctg agtaatctca accgccaaat ccatgccacc cataaaacga tcggccaacc
    13261 taaggttgag gttatgcggc agcgcatcct ggacatcaat ccacgggcgc aaataaataa
    13321 ttatcagcac ttttatctgc ccgatcaggc ggaagatata ttaaatgcag attatgatta
    13381 tatcattgat gcggttgata ccgtcaccgc taaaattgat ctgatcgttc aggcacagca
    13441 gcgtggcatc ccggttataa gctgtatggg tacgggcaac aagcttgacc cgggtcgcct
    13501 gcaaattgat gatatttaca gtacttccgt atgtccgctg gccaaagtga tgcgccggga
    13561 actgcgccgg cggggaataa ctgcgctgca agtggtttat tcccgggaag agccggtcaa
    13621 gcctcaggta gaggaggata atggttctga aaatgaatgt gaatcctgtt taaaaagcgg
    13681 aatttgctcg ggcaagcgca atcttcccgg cagcataact tttgttcccg cagtggccgg
    13741 tctgctgctg gccagccaag tggtcaggga tttaattgcc ggcatgtaat aagcaattgc
    13801 cgatgggcgg ggcgggctat gaaatgatta ggttgagtat ggttttgaga ttcactcttt
    13861 ataaggagag gattacattc ataacgcaaa cgaaatacgg tggttttaag agataattaa
    13921 aaaagtgaaa gtatatcaag ggaggtaaaa aataatggct aaaattgcaa cgcaaataac
    13981 tgacctgatt ggcaacaccc cgttgctgga actgggtgcg tacagccggg aaatggattt
    14041 gcaagcccgt ctgatcgcca aactggaata tttcaacccg gccggcagcg tcaaggatag
    14101 agttgggtat gccatgatca aggatgccga agaaaaaggc ctgctcaaac cgggttcagt
    14161 tattatagaa cctaccagcg ggaataccgg ggtgggtttg acatttgttg cagtcgccaa
    14221 aggttatcgc atcatcatcg tcatgcccga cagtatgagt atggaaagac gcatcctgat
    14281 gaaggctctg ggagctgaac tggtgctgac gccgggggcg gagggcatgc ctggagccat
    14341 caaacgtgct gaagaactgg cgcttgagat cgaagattcg tttatccctc agcagtttga
    14401 aaacccatcc aacccggaag cccaccggca gaccaccgtg caggagatct tgcacgacac
    14461 tgatggacag gtggatttct ttgtagccgg ggtcggaacg ggtgggacca ttaccgggat
    14521 tggagaagta ttgaagcgat ataatccgca gatcaagatc gtggccgttg agccgtttga
    14581 ttccccggtg cttgccggag ggaaagcggg accccatggg ctgcagggga taggcgccgg
    14641 cattatcccc aaggttttaa atgttaatat cctggatgaa atcattccgg taaaaacaga
    14701 agaggcctat gcaacggtgc accgcttggt aaaaacggaa ggactgctgg tgggcatttc
    14761 ttccggtgcg gctgtttttg ctgctgcaca gatcgccaaa cgggcggaga acaagggtaa
    14821 gcaaatcgtg gtgctgctgc cggatagcgg ggaacgctat ctatccacgc cgctgttcgc
    14881 atagacggat gcaacatgtg atgtatttgc atgcccgcag gccaaatcac ttgtccgtgt
    14941 cctggactga caaacactgg acggagatgg cctgccccag caggcggctg aggtatatcg
    15001 tttaaaaatt cgttagaatg aggttatgat gatggaaatg gatctcaatg aaaaactcgg
    15061 tcaattgatt tacaatctgg gttcgatgag cggtttggca gtcggatttt ccggcggggt
    15121 ggacagcagt ttgctgctca aggtggccca tggagtgatg gggcggaatg tgctggcggt
    15181 catcgttgat accccgacca tgccaaggcg ggaactgcag gacgcgctcg attttgtcaa
    15241 acaagcccag gtcaactggc aggtgatccc cgcagatcat ttgcagatcg agggctttgc
    15301 cgccaatcct gccaaccgct gttatttgtg caaacgggag atgtttggcc ggataaaaaa
    15361 agtcgctcag gaaagccatt tggaatggat agctgacggt tccaacctaa atgatctggc
    15421 ggaggatcgt cccggcatgc aggccttgcg cgaattgggg atcatcagtc cattgctgga
    15481 agccgatctg accaaggagg agattcgcca actggccaga cggatgcagc ttaaaacctg
    15541 ggataaacat gccaacagtt gtctggctac ccggtttgcc tgcgggcagg aaatcagtct
    15601 gcccaagctg cgccaagtgg aagaagccga agcttatctg aatgattgcg gatttcggca
    15661 aatcagagtc agaagttacg atcatgctgc ccgtatcgag gtatctccgg aagaaagagc
    15721 taaattcggc gatctgcagc tgatggatgc ggtccatcag aaactaaaag aattaggttt
    15781 cgtccatgtc agtcttgatc tgcaaggcta ccgacaagta aaggcagaga agcaaccatg
    15841 aaagagacaa aaaatccctc cgcagttgtc aggagggatt tctttttagg gggcaaccgt
    15901 gttgccagcc gatctgtcta aatatccagg tcagagtctg ctcccgggcc ggactgcccc
    15961 ttgaatacca gatcaggagc tttcaggctt actttggtgc cggccggtac ggatctggtc
    16021 aaaaagacgt tactgccgat cacgacacct ttaccgatga ctgtttcgcc ccccaggatg
    16081 gaagtcccgg agtaaacggt aacatcatct tcgatggtgg gatggcgttt gactccccga
    16141 agattctgac cttcgcgcgt ggacaacgca cccagggtga cgccctgata gaccttgaca
    16201 ttgttgccga tggaggtcgt ctcaccgatg acgacgccgg tgccgtgatc aataaagaaa
    16261 tacttgccga tggcagcacc aggatgaata tctataccag taacgctgtg ggcatattcg
    16321 gtcatgatgc ggggaatcag cggcaccgcc agtaattgca gttcatgcgc catccgatac
    16381 acgctgacgg cgaagacgcc cggataggaa aacatcactt catctttgct ggtcgctgcc
    16441 ggatcgccgt caaaggcagc ctggatatcc agctcgatac tttttctaag cgcaggaatc
    16501 ttgtctaaaa atcgatagat aatctcgctg gcttgtgcct caacatcatc caacgggata
    16561 tctttgctga tggcctgatg cgtcagcgcc agaacgattt gctcgtggag gatgcggtgc
    16621 aaattaatca gtaaatcacc gatatgatat tcgatggtgg tactgattag ctgccgctgc
    16681 ccgaaatact cgggaaagag taattcccgc agcataaaga taaccttgac gatgacctcc
    16741 cgtcgaggct gggatacaga ttccatggaa actgcggtct gattctcccg ataactgttg
    16801 acgatcgctg caaccagttg actggtctgc tgtgggttta gtctgctcac gtttttcacc
    16861 ctcattcatt cttgccggtt cacatggata taacgctgta ttatttaaga tattcccacc
    16921 tttaaaccta tagcaattat aggagataaa tgttcggata gcaattctgt gacagaaatc
    16981 aaaaacgggg aagcccttga gcttccccgt gatgatactg agttttagac aaaaaatgct
    17041 tcttccggaa tctccagggc gcttttgtcg ccgtttttaa agatctggct gaccgtttca
    17101 atctcaggca cgatattgcc gatatagaaa cgggcagagg caattttacc ggcatagaag
    17161 gcataatcaa aatgatccgg acccaattca tcaactttct tttgcgccag caaggcctgg
    17221 tcaaggatca gtttgccgcc cagcagcttg gctgtggcat gcaggatgcg ggtggagaac
    17281 aaaggcatca ttctgacttt gccttcgccc atatatccgc ccagtgttcc caggattgcc
    17341 tggtagctta gcagggctgc atccatgatg gcaaattctt tttcaaaacc agcggcgccg
    17401 cgatttgcgt tcaggaaatc actgatttgg cttaaccaca gaccaaacac ctggcctttg
    17461 gccatcgtaa acttgcggcc taccagatcc agggactgaa tgaagttggt gccttcccag
    17521 atgggataaa tgcggcagtc acggtacagt tcggcaatcg ggtattcctc cgagaagccg
    17581 tagccgccgt agcactgcat ggcattggag atacattcga tcgccatatc gctgcaatag
    17641 gccttaatga gggggatgtt gacgctcacc aaggcatcac actgtttggc caattctttg
    17701 tcggaactgt tgtaggcgat gtccagcaga tagaaattgt aaaggatcag ggcacgaaat
    17761 acttccacat gagacttctg gaacagcagc atgcgacgaa tgtcttcgtg ctcgatgatg
    17821 ggcacccgat tgcctttggg attggtgaac ggtctgccct gaatgcgttc tttgcagtag
    17881 gcaacggcat tatggtaagc taccgctgac agggagagcg ccccgtgtcc ggtcatcaga
    17941 cgctcttcgt tcataaccct gaacatttgc atcatccctt tgccgactcc gtcttcaccg
    18001 gggggatctc cgaccagcca gcctttgcat tggccgtttt cgccaaaatt caaaacgcag
    18061 gtgacgttgc ccttgatgcc catcttgtgt tcgataccgc tgacgcttac gtcgttgggg
    18121 ccaaggatgg tgccgtccat ttcactgaag tactgtggta tgacaaacag ggaaagacct
    18181 ttggtgccgg gcgcggctcc ttctatacgg gccagcacca aatgaatggt gttgtcacaa
    18241 atgccgtttt ctgcatggct gataaaacat ttggttcctt tgatcatata aaccccggtc
    18301 tcatccgttg gataggcttt ggtggtttga tcgccgacat cggaaccggc tgatggttcg
    18361 gtcaaacaca tggtggcagt ccattccccg ctgaacattc tgccggcata catattgatc
    18421 aaatattcgc tgccaaactc ttcgagcact tcaccgccgg cagccggacc ggccaggatg
    18481 gttccgatat gtggattggc gccgatcatg aattccatca tggcccacat cacgctcaag
    18541 ggcacggcgg agggatcatt tctgtcttta ttgctgctgc agaaaccgtt ctgctgaata
    18601 aaatgataag ccgtcttgca ggattcggtc atacgggcat gaccgtccac gtaaatgccg
    18661 ccgattttat cagcatcttc attggtaggg gcgacaactt ctttggctcc cttcagggca
    18721 ttgtcgagga tgaaatcgat atcatcgacg ctgtaacctc cctggaaccg ttctgtttcc
    18781 agaaccttgg tcaagtcgag ccattctttt agaataaatt tgtggtcgcg ggtggtgttg
    18841 ataaagtttg aagacattaa ttaccctcct tgttttatta tgcttggctg gtggttttct
    18901 caatagaatt gctgcgtatc ttttgcaagc gattgctgtg acgccaatag tatattaaaa
    18961 aaatatcctg gaatataatt gttgttaata aatattacct taacaggacg attaattcaa
    19021 tgggaaatgt ccgtcaaacg atgaaaaaac acttgacgag cacctttccg tcaagggggt
    19081 gggcatataa aaaagccagc cggcagctgg cttgattaaa gctatgtacg tttaatctcc
    19141 ttccgcataa ggatattttt tatcccatga acgggaaaaa cttgggaact tgaccaataa
    19201 agcctgatag attacagcct gatactcgtc attcaacgga ctgaaaacat cacttaaatt
    19261 ggcgtagttt tcactgttgt aaaggttggc agcgactttt aataacgccc ggtcggcagc
    19321 atctccggtc aaggtctgcg acagcgattc aaaatcaatg tcggtgggga gaaagtattt
    19381 gtctatgatg gcaaaggaac cttcggaatt tcccaaggta ccgatcgccg ctaatacata
    19441 cgcgataggc gtgatgtcag gatagttttt gccagctttt tttatcaggc gcagaaaatt
    19501 tcccttgtga aatttgttga taaagtagac gggttttttc actggataat cctcctttgt
    19561 tcaaaatatc ctacggtata taatgatgct agcacatttt tattattttt tatatatgga
    19621 accggccaaa ttattatatt tcgatataat ttattactta gtatcaatac gttttggtaa
    19681 aagtatactt gttggtttgc tttttgacag tttcatatca atatgccaag aaagaaggaa
    19741 gacaaatgaa ggatttcagt caatccgaac aggaaccgga agagaagtta aatatcaagc
    19801 ttggggtggc ccctaattgt tttgttttca tgcagggaag aatgattgat ttcaatggaa
    19861 atgagcggtt gacgattgcc tttccagttt tggaaagcta tcttaatccc ggtggaagta
    19921 tgcagggagg ctttatcacc gcagcgtttg acaatgtatt cgggccgttg tgccaggcag
    19981 tgactggcac caaggcgacc accacggttg atttgtatac gaattatcat cgcccaattt
    20041 ttgccggaga tgaacttgtc ataactgcat ccatcattgt caagggaaag accaagattc
    20101 acatgacggg tgcggcatac aaccaggcca atcaattgat tgccacggcc agcaccaatt
    20161 atatctattt aaaagcaccc cgctccggcg catagagcag cgtaatcacg ttccctgacg
    20221 agcgggcagg ttccgtcaaa ctataactaa aaattatttt aaagataagg ttcggtgtga
    20281 tatattaggg atagtttaaa tcgggaaagg tgcttgcaac aaatcatgga agttggcata
    20341 agctaacata atcccaaatg caaagacagg caggcaggtc acggaagggg aacaacagat
    20401 gcccgcaatt tatatggata atgcagcaac tacgtatccc aagccagata gtgtttatcg
    20461 ggcagtggat cattttaatc gtaatatggg cggcaatccg gggcgcggca gcagccagca
    20521 gtcgatagag gccggctcgg tgattttgca aacgagggaa gcgctggcac gtttttttaa
    20581 catcaaggat agccttgaca tcgcttttac tgccaatgtg accgaatctc tgaacattgg
    20641 tttgaaaggt ttgctgcgcc atggtgatca tgtgattact tccagcatgg agcacaatgc
    20701 ggtggcccgg ccattgtatg cgctgcgccg gcatggcatc gaatggacgc aggtggcctg
    20761 tgctgctgac ggcagccttg atccgcagga tattgccagg gcaatcaaac ccaatacgcg
    20821 tatgatctgt acgctgcatg cgtccaatgt caccggcacc attttgccca tccgggaaat
    20881 cggccgtctg gccagggagg ccggcattat ttatatggtc gacagtgccc aaagcgcagg
    20941 ggtgctgccc attgatgtgc aggccgatgc cattgacatc ctggcttttg
//
LOCUS       DAOTHZ010000036        20954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3042_length_20954_cov_6.906981, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000036 DAOTHZ010000000
VERSION     DAOTHZ010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 20954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20954
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_20954_cov_6.906981"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /gene="porA"
                     /locus_tag="P5549_05780"
     CDS             <1..552
                     /gene="porA"
                     /locus_tag="P5549_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRW12616.1"
                     /translation="AYYMEHKVLQAEAMKAAKARILEVAAAFEKISGRRYGLLEEYRM
                     EDAQLAVVLIGSSAGTAKAAVDKLRDEGVKAGLVKIRVFRPFPGEELARALAETKAVA
                     VMDKSEGFSANGGPLFAETRSALYDLPQRPHMINIVYGLGGRDFKTKDVKKVFARLAG
                     IATTGEIGPIYTHLGQRSREDQA"
     gene            549..1487
                     /locus_tag="P5549_05785"
     CDS             549..1487
                     /locus_tag="P5549_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRW12617.1"
                     /translation="MSTIYNLKHEVEKPVRLTGGHRLCAGCGAGVVVSGVLRALDNED
                     RAVVVNATSCLEVSTFMYPYTAYMDSYIHSAFENAAATCSGVEAAYNALKRRGKVDGI
                     VKFITFAGDGGTYDIGFQSLSGAMERWHDMVYVCYDNEAYMNTGIQRSSSTPGFARTT
                     TTPVGSVGQGKKQNKKDLTEIMVAHGIPYVAQTAPLGNFKDLHTKANKAIYTEGPCFL
                     NILSPCPRGWEYPMAKLAEILKLAVDTCVWPLYEVEEGSWRISYTPKKKLPVEDFLRP
                     QGRFRHMFQKGNEWMLEEAQTYVDQKWERLLERTGS"
     gene            1559..2467
                     /locus_tag="P5549_05790"
     CDS             1559..2467
                     /locus_tag="P5549_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6B methylase"
                     /protein_id="HRW12618.1"
                     /translation="MEQTMFSNEVILSWLQYFSQNVDINLAKLKMLDITGKNKNLIPT
                     VMTNRAVLVFTDAGHPDIFYDMWNSELGDCEIWYNEGSEPSGEIKHNKVSDMINRGIN
                     ASAAMLILNPDAATNYQVGLENSRFSCGSVQYVCREVRAIIMNKLNPADQDNICIISG
                     ESIAVEAAMIAAEGNIIAVEYDRHDRNTMKCNIEKFGLNNVVIVESLADGILAGLPVP
                     NKAFIVASPRIDSEIKSLLVVNPSMDIVVYTLDFEILTALPSLFRENGLERTEAIHTA
                     VSRVNSKKMMEPFPAPWIVSAKSIKV"
     gene            complement(2571..3323)
                     /locus_tag="P5549_05795"
     CDS             complement(2571..3323)
                     /locus_tag="P5549_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRW12619.1"
                     /translation="MKLISWNVNGLRSCLNKGFMDFFLAEQPDICCIQETKMQPGQAD
                     IMLEGYEMFWNSAEKKGYSGTAIFSRLKPQSVMLDLEQPEHGTEGRIITLEYNDFYLV
                     NAYAPNAQRGLARLDYRLAWQNDFQEHLTRLKQNKPVIVCGDFNVAHQEIDIKNPKSN
                     HNSAGFSPQEREKMTELIASGFIDTFRFLHPDQAGAYTWWSYMFNAREKNVGWRIDYF
                     LVSGGIKDQIKDAMIYTDVLGSDHCPVGLEIW"
     gene            complement(3399..3761)
                     /locus_tag="P5549_05800"
     CDS             complement(3399..3761)
                     /locus_tag="P5549_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12620.1"
                     /translation="MSSDKMMRNCFAVNGSSLLLPEQKIVFSYDIRSVKMANDLYIVL
                     LSIPLDDPMSRNLYAVKPSGEIDWQVEDPGHWVKDPLPFENLFIDDDKTLRVSDFYGR
                     VFQIDVHNGQIQNKKSIK"
     gene            complement(3758..3982)
                     /locus_tag="P5549_05805"
     CDS             complement(3758..3982)
                     /locus_tag="P5549_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12621.1"
                     /translation="MKSRDIEGLAPEQIKDLFALPAVPVYQVDVIIKAGTHLHSGLTN
                     RNEGWGNGGGIQFDLMGQRTGEFLHERPLP"
     gene            4104..4811
                     /gene="hypB"
                     /locus_tag="P5549_05810"
     CDS             4104..4811
                     /gene="hypB"
                     /locus_tag="P5549_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HRW12622.1"
                     /translation="MSQVRLIEIKEEILFANRHRADEIREKLRRNRVFMLNLMASPGA
                     GKTSLIIETVRRLKDVFRIAVIEGDIDSMVDSEKVSAEGVPTVQLRTGGACHLDAPMI
                     HLALKTMDLENIDLIIIENIGNLVCPAEFDTGAVMKAMILSVPEGDDKILKYPLMFSV
                     CDALVVSKTDYLEGPGADFCIDRLRNRAGALAPTMKMFQTSCRTGAGLDEWCAWLAKE
                     ITDFMAANDNDKSLPED"
     gene            5018..6406
                     /locus_tag="P5549_05815"
     CDS             5018..6406
                     /locus_tag="P5549_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRW12623.1"
                     /translation="MDRTEEMRSGSLGRLLFKFSLPAIVGMLVNSLYNVIDRIFLGRG
                     IGQLAIAATTVAFPMMTILMAVSILIGVGATALISIRLGEQRKEEAEKIAGNAVVMIM
                     LLPALCAVFFFLFTEPVLIFLGASQEVLPYAKDFTEIIMLGSVFGSFSMGVNNFIRAE
                     GNPKVSMYTQLLGAFCNVILNYVFIFKFGWGMKGSAFATVLAQFISALWVLSYFLTGR
                     SLVKLKLSNMRLQWPILTSIMAIGFAPFAMQLAASVQQVILNRTLMVYGGDIALSAIG
                     IIMSVATLLFMPVLGVSQGAQPIIGYNYGAQQYDRVKGTWKLAVLAGTAIALTGYVTI
                     HIWPEQLVGLFSEGDTALTALTTHAMLVYMALIPLIGFQIVSSTYFQAVGKARQAAVL
                     SLSRQILFFIPLLLILPRFWGAEGVWRSAPISDLLAVCLSATMMFFEMRSLRKTALNH
                     PAIKQLGDEPIK"
     gene            complement(6437..7243)
                     /locus_tag="P5549_05820"
     CDS             complement(6437..7243)
                     /locus_tag="P5549_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12624.1"
                     /translation="MSPFGNVMQLEMGKEVNGQVLYSMAAYYVDGILFDTGPYSVTDE
                     AAKIFPSLVVEKVINTHHHEDHIGNNYLLDKQGIPIFAHDKAVALIENPSLWTDRLLD
                     YQKLVWDYPPASRCNPLGEYLSSRHYLFKVIHTPGHSVDHICLLEEEQGWLFSGDLFL
                     GEKVKQLRSDEDIHLSIKSLECLLRYQFDTIFCCSGRVFKDGWHKMQAKLDWWQDIRQ
                     QVIMLGEQGLQDEAIRDQLLGPENLLAQITEGDLSRLNLIRSFNNRNIMN"
     gene            7495..7845
                     /locus_tag="P5549_05825"
     CDS             7495..7845
                     /locus_tag="P5549_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRW12625.1"
                     /translation="MFIDQIKEIAGQKIHHPGVMENVIKKVLIGPKQGWEGWTMRQFD
                     LAANGFTPRHTHPWPHINYVISGQGLLYLDGHDYEVGPGSIAYVPDNVEHQFKNIGSE
                     DFSFICIVPEEGDK"
     gene            7937..8884
                     /locus_tag="P5549_05830"
     CDS             7937..8884
                     /locus_tag="P5549_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRW12626.1"
                     /translation="MRKLLTILLVMFFTAGLAGCGETGNNAAEMGEHKTITIGVMPDV
                     ESIPFIIAEKNGYFSKADVQVKIEHFTSAKDRDSALQTGRLDGVITDVLAVVFANEGG
                     IDLKMIARSEGNILLMAGREAIIASLDDLPGKSVGLSTNTIMEYTVDKMLATEQIKAE
                     EINKIAIPPLPTRLEMLQGGKIDAAILPEPLAGLALKDGAVVLSSTDQMHHKAGAIAF
                     TGKSLKENPDEIKAIFAAYNEAVDYLQKQPRESDVEFIIQEQGFPSAIKDSFTLPHYT
                     HAEALDARIVAEVVQWMLSKNLIKQSYAYEDLTAEDILR"
     gene            8892..9629
                     /locus_tag="P5549_05835"
     CDS             8892..9629
                     /locus_tag="P5549_05835"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12627.1"
                     /translation="MIDIKDLSVRYGSPDNAVLALEQININIGQGEIFTFIGPSGCGK
                     STFLYVLSGIIRDYEGTVLINGRPVDPRQQRIGLILQNYGLLPWKNVRQNIVLGVKIK
                     GQPREEEYFDYILKQLGIDQLLDRYPKELSGGQQQRVAIARAFLLKPDILLMDEPFSA
                     LDAISREEMQELFLKIWQAHHLTTVFITHSVDEALYLGSKIVVFSSSPGRILGIYENP
                     CFQRDNLRYRDEYYQMSRQLRNLLDKG"
     gene            9635..10375
                     /locus_tag="P5549_05840"
     CDS             9635..10375
                     /locus_tag="P5549_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW12628.1"
                     /translation="MQSSEKWKGALYSFVLVIVVWQAVAMLLKMPIIPTPTAVFVNLV
                     EVFQIRMEIHLWYSLGRIVAGIAISILLGVSLGLLMGYFAKVDKIFSPLVYFTYPVPK
                     IALLPIVMLLFGLGEASKLIMIVLIIVFQIVITARDAVKNIPQEMFRSLQSLGAGRRQ
                     IFKEIIFPASLPEVLTATRLALGTAVSILFFTETFGTEYGMGYFIMDAWMRVNYLDMY
                     AGIVMLSLLGFCLFTLLDVAEQYFCSWR"
     gene            10419..11333
                     /locus_tag="P5549_05845"
     CDS             10419..11333
                     /locus_tag="P5549_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRW12629.1"
                     /translation="MPIATRRAKGNAQVIIDHEHCSLCGLCVEVCKGKPLFIQGDRVC
                     VDQSILFGCIGCGQCAAVCPQRCIIIEGRDLSAEDLLEMPPREQWADYTQLHTLLQGR
                     RSTRNFQRREVEREVIEKILDTVATAPMGLPPSEVEILVLHGFNRVREFSQDIVGVFE
                     TQKWMFSPPALALMRPFIGKDVYGLMKTFVAPLPKFFSEKMARGEDWLLYDAPLAMYF
                     HASPTADPMDYAIAATYAMLAAQSLGLGTCMIGSVAPFLKYKNKINNKYGIPKDNQQG
                     LMVIFGYPQYKYTRSIKRSLGNVKFYEG"
     gene            11388..14816
                     /locus_tag="P5549_05850"
     CDS             11388..14816
                     /locus_tag="P5549_05850"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012704538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRW12630.1"
                     /translation="MKAFKKVLIANRGEIAIRIIRACHELGMRTVSVYSKEDQLALFR
                     TKSDESYLIGNNKGPVEAYLAIEEIINLAIKKGVDAIHPGYGFLAENPEFAKKCIESG
                     IEFIGPTPEMMDKLGDKIKSKLIAQEIGVPTIPGVEKPIKSDREAIRFAKQCGYPIML
                     KASAGGGGRGMRIVRSEEDLLREFHSAQNEARKAFGSDEIFIEKYLDSPKHIEVQVLG
                     DNYGNIVHLFERDCSIQRRHQKVIEFTPAIGISETQRQAICADAIKVAQSVNYRSAGT
                     VEFLVDNQGQHYFIEMNPRIQVEHTVTEMVTGIDIVQSQILIAEGHRLDSQVVGIPDQ
                     SAIQLRGYAIQCRVTTEDPNNSFAPDTGKLDVYRTGSGFGIRLDGGNGYTGSVISPYY
                     DSLLVKIIAHSRTFEDTIKKAQRSIRETVISGVKTNEAFLLNVLSHPTFVRGECDTGF
                     LDATPEIFDITPKADREASILQYISEKVVNEGKGIRKDYDVPKIPKFNSEEVLVGSKQ
                     ILDSRGPHGVVEWIKAQNKLLMTDTSMRDAHQSLMATRIRTVDMLRIAEATAHLGQDL
                     FSLEMWGGATYDVAYRFLKESPWDRLTQLREKIPNIMFQMLLRGANAVGYTNYPDNVI
                     REFIQESANAGIDIFRIFDSLNWLKGMEIAVDETLKSGKIAEACICYTGDILDDSRDK
                     YSLRYYVETAKEIEKMGAHILGIKDMAGLLKPYAAEKLIKALKDEISIPIHLHTHDTS
                     GNGVATLLMAAEAGVDIVDTAFNSLSGLTSQPALNSVVAALENTNRDTGINLDDIQEI
                     SDYWADVRPYYSEFESGLKSGTADIYKYEIPGGQYSNLKPQVDSFGLAHKFKEVKEMY
                     KTVNEMMGDIVKVTPSSKMVGDMAIFMVKNDLNPKNIYDKGQDLAFPDSAVAYFQGMM
                     GQPVGGFPQELQKLVLKGIEPITCRPGELLEPVDFYASSKYLEERYGFQPNRRALLSY
                     ALYPKVYEEYIEFKKQYGDLSRMNSPVFFNGISEGEVCEVEVEEGKTFIIKLVAIGKI
                     DKEGYRKIYFEVNGNQRAINILDKSYQKGEAATATVMADPDNKNEIGASIPGTVARIL
                     VKEGEQIKPGQNLIVIEAMKMETQIIAPVGGEVTFINVTEGRQVKNGELLLKIE"
     gene            complement(14877..16070)
                     /locus_tag="P5549_05855"
     CDS             complement(14877..16070)
                     /locus_tag="P5549_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRW12631.1"
                     /translation="MATPKYFVWEYRTLVEVGSGCRTFAAQRFMEMGCKRVGMITDKG
                     LIDAGVADMVKDVFAIQGGVKMAGVYDKIEQDAAMRVINDCARWYRENAFDGMLVVGG
                     GSVMDSAKGVKMLLGGGATDIKNLMPGNLGPYIRPLGKPLGVPNIFIPTTAGTGSEVS
                     PLAVIYNEADKVKGDVLHPYVPADIALLDPDLTVGLPPAMTVSTGLDALGHAVEGLTS
                     PEANIMADSLNIHSIKMIMQYLPIAASDGKNLEARTNMLAASNMAIMGFAMGGMMFPV
                     HNIAHAAGGQLRIPHGQAVGVALPACMEHMPWHYWPKAKALAEGFGVRSDSPDPAELV
                     ALAREKVLSLMKECGFKPYFENTLDASQQETLFWAIKADPTAMLYPIPDDTIKAIIGS
                     IFPTI"
     gene            complement(16087..17613)
                     /locus_tag="P5549_05860"
     CDS             complement(16087..17613)
                     /locus_tag="P5549_05860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRW12632.1"
                     /translation="MLVSKEILEYQLYIDGQWTAGESGDMMDVINPANQEVVARVPRA
                     TQGDIDKALHSARAAFESGIWSGKSIDERIEVLTRATMAVLANKDRLAWLEALTSGAT
                     LRRTGFVDVMSVAGSLAMSAAILKQMPEVEHAICPPWVAPMHSYAIREPIGVCAGITP
                     WNFPMILAAYKFAPALAMGNSIVMKPASITPVTTLELAKILSDAGIPPGVFNVVTGPG
                     ASIGNYLAAHPQVDKIGFTGSTEVGRQIAHLAADGIKRVTLELGGKSPLIVLDDADIE
                     MASSMTLLAFLTHSGQVCESGTRLIVPRKMQDQLVDAIITKIKKLNIGDQMDLATDLG
                     PIASSQQLQTIMNYVKIGQKEGAQIAYGGNRLSGGIYDNGFYHEPTIFINCSNQMQHV
                     REEIFGPVQCVIPYDDLDEAIAIANDSPYGLGGGVCSTNTTRAQKVAKRMRTGSVWIN
                     TWHILRPDVPFGGYKESGYGRENCYHALMAYSEVKHICQDLTPDAKSSFIYTFLGFNR
                     "
     gene            complement(17784..19181)
                     /locus_tag="P5549_05865"
     CDS             complement(17784..19181)
                     /locus_tag="P5549_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12633.1"
                     /translation="MNKDFHQMYKDKLRTAEEAMKVVKSGAWIDYAMFNGKPVACDTA
                     LAARKDELKNINVMGAVTVPPLPEVVMKDPMGEVFTYNDQHFSALTRIFQERCGNVFY
                     QPTLYGESEMYMKYGNYEEDPGSKKSKIGTNLRDVFIAQTTPMDENGYFNWGLHNSCC
                     YLQAVTAKNRIVEVNQNIPYCYGGNMERIHISQVTYVVEGENPPMYELAKIDPTDVDA
                     KIAANVLEYLQDGQCIQLGIGAMPNLLGTMISETDLKDLSGWTEMLVDAYMHMWEAGR
                     MTGIKKIFDPGKINYTFALGGKELYDWMDRNPAIASCSVGYVNHPMQLAQIDNLISIN
                     QALQVDLYGQVNAESSGSKQVSGNGGMSDFVLGAFWSKGGRSFICLPSTHTKKDGTVI
                     SRIVPNFEPGSITTLSRQMINHVVTEYGVISLKTCPTWYRAEKLISIAHPDFRDDLIK
                     AAEERKIWRRTNKIE"
     gene            19418..20197
                     /locus_tag="P5549_05870"
     CDS             19418..20197
                     /locus_tag="P5549_05870"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRW12634.1"
                     /translation="MKVLEMFKLNGKTAVVTGGSIGLGAYMATALAEAGANVVIAARK
                     VDRCIELAEELEKLGVKTLGVACDVSKAEDCQNLVDVTVKEFGSLDILVNNAGLTWGG
                     DSMTFAMKDWHKVMDLNLHGLFQLTQMAGRVMREQGYGKIINICSLGGYGGTKPEEMD
                     AVAYNASKGALMTLTKDLAVKWARYGIYVNALAPGFFPTHMTEWILEKNSKYLLPRVP
                     FGRYGGEDDLKGAVVFLASAASNFITGQIINVDGGEMAMVP"
     gene            20530..>20954
                     /locus_tag="P5549_05875"
     CDS             20530..>20954
                     /locus_tag="P5549_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12635.1"
                     /translation="MNHKTAASILAVLLFFGVCIYISGIIKPLDKLAGSERTNEIKTT
                     QNSLEQRASVDKEVTVKRTDSQKKTKKVQSPAPPAEPKIVQVQTENSVQDKSSNPSSG
                     TTSVNQHESSAASNPAGSGSNPVSQGNSTNSSNNQVSRG"
BASE COUNT         5600 a         4581 c         5254 g         5519 t
ORIGIN      
        1 gcttactata tggaacacaa ggtattgcaa gccgaggcca tgaaggcggc caaggcccgc
       61 atactggagg tagcggcagc gtttgaaaag atttccggac gccgatatgg cttgttggag
      121 gaatatcgaa tggaagatgc ccagcttgcg gtggtattga ttgggtccag tgcggggaca
      181 gccaaagcag cggtcgacaa gctgcgagac gaaggagtca aggccggatt ggtcaaaatc
      241 agagtgtttc gtcctttccc gggagaagaa ttggcccggg ccttggcgga aaccaaggct
      301 gtcgccgtga tggacaaatc cgagggtttt tccgccaatg gtggtccctt gtttgccgag
      361 acccgcagtg ccttgtacga tttgccgcag cgaccgcaca tgatcaatat cgtttacggt
      421 ctgggtggtc gtgattttaa aaccaaggat gtaaaaaaag tatttgcccg gctagccgga
      481 attgcgacga ctggggagat tggaccgatc tatacgcact tgggacaaag gtcaagggag
      541 gatcaggcat gagcacaata tataatctaa aacacgaagt ggaaaagccg gtgcgactca
      601 ccggaggtca tcggctttgc gccggctgtg gtgccggggt ggttgtctcc ggagtgctgc
      661 gggcattgga taatgaggat cgggcggtgg tggtcaatgc caccagttgt ttggaggttt
      721 cgacctttat gtatccgtat accgcctata tggacagcta tattcactct gcttttgaaa
      781 acgcggctgc gacttgttcc ggcgtagagg ccgcttataa cgcattgaaa cgccgcggca
      841 aggtggacgg gatcgtcaag tttatcactt ttgccggaga tgggggcacc tatgatatcg
      901 gctttcagtc tctgtccggt gcaatggaac gctggcatga catggtttac gtctgctatg
      961 acaacgaagc gtatatgaac accgggatcc agagatcttc ctccacgcct ggttttgccc
     1021 gcaccaccac cacgccggtc ggctccgtgg ggcagggcaa gaagcaaaac aagaaagacc
     1081 tgacggagat catggttgcc catggcatcc cttatgtagc ccagactgct ccgctgggaa
     1141 attttaagga tttgcacacc aaagccaata aagccattta taccgaaggc ccttgttttc
     1201 tgaatatact gtccccatgc cctcgtggct gggagtatcc catggccaaa ctggcagaaa
     1261 ttctaaagct cgcagttgat acttgtgtct ggccgcttta tgaggtggag gaaggttcgt
     1321 ggcggattag ttatacgcct aaaaagaagt tgccggtgga ggattttctc cggccacagg
     1381 gcagattccg ccatatgttt cagaagggca atgagtggat gctggaagaa gcgcaaactt
     1441 atgtagatca aaaatgggaa cgattgctgg aacgtacagg ttcttaaaca attgaccaga
     1501 aatatggtaa taaatacaaa tcaatgatat gataactgtt aacaaggagg ttttgcctat
     1561 ggaacagaca atgttttcaa atgaggtgat tttgtcctgg ctccagtatt tttctcaaaa
     1621 tgttgatatc aatctggcaa aactaaagat gctggacatt accggaaaaa acaagaacct
     1681 aatccctacc gtcatgacca accgggctgt attggtcttt acagatgcgg gacatccgga
     1741 tatcttctac gatatgtgga attcggaact gggtgactgt gaaatctggt acaacgaagg
     1801 ttccgagcca tccggtgaaa taaagcacaa caaagtctcg gacatgatca atcgcgggat
     1861 caatgcatcg gcagccatgt tgattctcaa tcctgacgca gctaccaatt accaggtggg
     1921 cttggaaaac agccgtttct catgcggctc ggtacagtat gtctgccggg aggtgcgtgc
     1981 catcatcatg aacaagctca atccggctga tcaggacaat atatgtatta tctcagggga
     2041 gagtattgcg gtggaggctg ccatgattgc agctgagggc aatatcattg cagtagaata
     2101 tgaccgtcat gatcgtaaca cgatgaaatg caatattgag aaatttggtc tgaacaacgt
     2161 tgtcatcgtt gagtctttgg ccgatgggat tctggcagga ttgccggtgc ctaataaagc
     2221 ttttattgtg gcttctcccc ggattgacag cgaaatcaag agtttgctgg tggttaatcc
     2281 gagcatggac attgtggttt atacattgga cttcgagatt ttgaccgctc tgccttcatt
     2341 attcagggaa aacggcttgg aacgtaccga agccattcat acggcagtct cccgggtcaa
     2401 cagtaaaaaa atgatggagc ctttcccagc cccttggatt gtttctgcca aaagcatcaa
     2461 agtgtaagct ggcgtactgc atcacaatcc agagaaatga tatggtttaa ccaggaaccg
     2521 cacccgatga tccgggtgcg gtcagttttt aagggttttt taacttttcg ttaccaaatt
     2581 tccaatccga ctggacagtg atcacttccc aggacatctg tatagatcat ggcatctttt
     2641 atctgatcct tgattccccc tgataccaga aaataatcaa ttcgccagcc tacattcttt
     2701 tcccgggcat taaacatata ggaccaccag gtataggcac ctgcctgatc aggatggaga
     2761 aaacggaaag tatcgataaa acctgatgca atcaactccg tcatcttttc ccgctcctgg
     2821 ggagaaaagc cggcgctgtt gtgattggat ttgggatttt taatatcgat ttcctggtgc
     2881 gccacgttaa aatctccgca gacaatcacc ggtttattct gtttgagcct ggttaagtgt
     2941 tcctgaaaat cattctgcca ggccagacga tagtcaagcc gggcaaggcc gcgttgggca
     3001 ttgggtgcgt aggcattaac cagataaaaa tcattatatt caagcgttat gatgcggcct
     3061 tctgtgccat gttcaggctg ctcgaggtcc agcatgacgg attgcggttt aagccgggaa
     3121 aaaattgcgg ttcctgaata gccttttttc tctgcgctgt tccagaacat ttcataaccc
     3181 tctaacataa tgtctgcctg tccgggttgc attttggttt cctgaatgca gcatatatca
     3241 ggctgttcag ccaggaaaaa gtccataaat cctttattca gacaagaacg cagtccgttt
     3301 acattccagg aaataagttt catatctttc tcctttagcg ttataggtat gaatattata
     3361 caacagttga ttccctcatt gtttaggtat tactaccctt atttaatact ttttttgttt
     3421 tgaatttgtc cattgtgaac gtcgatctga aaaacccgtc cataaaagtc tgagacccgg
     3481 agtgttttgt catcatcaat aaaaaggttt tcaaacggaa gcggatcttt cacccagtga
     3541 cccgggtcct caacctgcca atcaatttca ccggatggtt taacggcata aagatttctg
     3601 gacataggat catccagagg gatggacaga agcacaatgt acaaatcatt tgccattttc
     3661 acggagcgaa tgtcgtatga aaaaacaatt ttttgttccg gcaagagtag agatgaaccg
     3721 tttacagcaa agcaattgcg catcatttta tcactgctca tgggagaggc ctttcgtgta
     3781 aaaattcccc agtgcgttgt cccatgagat caaattgtat gccgcctcca ttgccccagc
     3841 cctcattccg gttggttagt ccgctatgca gatgggtgcc ggcttttatg atgacatcta
     3901 cctgatagac tggcactgcc ggcaaagcaa agagatcctt gatctgttct ggtgcaagcc
     3961 cttcaatatc cctgctcttc atcaaccagc ccccatattt gccggagaca ttgccatcat
     4021 tcttgccttg gtggacgtaa gcagcaattg tatgtggtag aatacacata aagctatgat
     4081 gccaatgaga tggaagtgat acaatgagtc aggtccgatt gattgaaatc aaggaagaaa
     4141 tattatttgc gaatcgtcat cgtgcggatg aaataagaga aaaacttcgc cgtaaccggg
     4201 tttttatgct caatttgatg gcatcgcccg gagccggcaa gaccagcctg attatcgaaa
     4261 ccgttcgtcg cctgaaagat gtttttcgaa tcgctgtaat cgagggtgat atcgattcaa
     4321 tggtggattc ggaaaaagtc tcggcggaag gggttccaac ggtacaactg cgcaccggcg
     4381 gtgcctgtca tctggatgca ccgatgatcc acctggcatt aaaaaccatg gatttggaaa
     4441 acattgattt gatcattatt gaaaacattg gcaacctggt ttgcccggct gaatttgata
     4501 ccggagcggt tatgaaagcg atgattctta gtgtgccgga gggggatgac aagatattaa
     4561 aatatcccct gatgttctcg gtctgtgatg ccctggtggt gagcaagact gattatctgg
     4621 agggacccgg ggcggatttt tgtatagatc gcttgcgtaa tcgtgccggc gcgctggcgc
     4681 cgacaatgaa aatgtttcaa acttcgtgcc ggactggagc cggattggat gaatggtgcg
     4741 catggcttgc caaggaaatt acagatttca tggcagccaa tgacaatgat aaatcactgc
     4801 cggaagattg ataatgatca tcgttccaac cggaaaggtt tcgaacatat gaggatgagt
     4861 tgattcttta ttttattgtg attagaattt atcttgtaat ataaccagat gtactaatcg
     4921 aatactgttc atcattttga gaggtcttta ttcaggcttc tgagagtccc cgcagttgac
     4981 caggtttttt attttgtttt ataggagtca tttatcgatg gatcgaactg aagaaatgcg
     5041 ttcagggagt ctgggccgcc tgctgttcaa gttttcgctg ccggcgattg ttggtatgct
     5101 ggtaaactcc ctttataatg taattgacag gatattttta ggtcggggaa tcggtcaatt
     5161 ggccatcgct gccaccaccg tagcgtttcc catgatgacc atcctcatgg ccgtttccat
     5221 tctcatcggt gttggcgcca ctgccttgat atccatccgc ctgggggaac agcgcaagga
     5281 agaagccgag aaaatagccg gtaatgcggt ggtcatgatc atgctgctgc cagccttgtg
     5341 tgcagtgttt ttctttttat ttaccgagcc ggtgctgatt ttcctggggg ccagtcagga
     5401 agtgttgccc tatgccaagg attttactga aattattatg ttgggctcgg tttttggctc
     5461 ttttagcatg ggggtcaata atttcatccg cgcggaaggc aaccccaagg tgtcgatgta
     5521 cacgcaatta ttgggggcat tttgcaatgt gatcttaaac tatgtgttta tttttaaatt
     5581 tggctggggg atgaaaggct cggctttcgc gacggtgttg gcgcagttta tttctgccct
     5641 gtgggtttta agctattttc ttaccggcag aagcctggtc aagctcaaac tcagcaatat
     5701 gcggttgcag tggcctattc taaccagcat tatggcaatc ggctttgcgc cttttgccat
     5761 gcagcttgct gccagtgtgc aacaggtgat tctgaaccgg acgttgatgg tttatggcgg
     5821 agacattgct ttgtcagcga ttggtattat tatgagtgta gcgactttgc tgtttatgcc
     5881 ggttttaggg gtatctcagg gagcgcagcc cataatcggc tacaactatg gtgcccaaca
     5941 gtatgaccgg gtcaagggaa cctggaaact ggcggttctg gccggaaccg cgattgccct
     6001 gaccgggtat gtgaccatac atatctggcc tgagcaattg gtggggcttt tcagtgaagg
     6061 agacactgcg ctgactgcgc tgaccaccca cgccatgttg gtctatatgg ccctgattcc
     6121 gctgattggt tttcaaatcg tcagttcaac ttattttcag gcggttggca aggcgcgcca
     6181 ggcagctgtt cttagtttgt cgcgtcaaat cttgttcttt atccccttgt tgctcatttt
     6241 gccgcgtttt tggggggcag aaggggtgtg gcgttcggca ccgatttcag atttactagc
     6301 ggtttgtctg tccgcaacaa tgatgttctt tgaaatgagg agtctaagga agactgcatt
     6361 aaaccatcca gcaataaaac aactgggcga tgaaccgatc aagtaatcgt aattgttgac
     6421 tccgttattg aagggttcaa ttcataatat tccggttatt aaatgaacgg atcaaattca
     6481 atctggagag atctccttct gtgatttggg cgagaagatt ctctggtccg agcaattgat
     6541 cacgaatagc ttcatcctgc aagccttgtt ctcccagcat gatgacttgt tgccggatgt
     6601 cctgccacca gtcaagtttg gcctgcattt tatgccatcc gtctttgaaa acccgaccgg
     6661 agcagcagaa tatggtgtca aattgatatc gcagcaggca ttccagggat ttgatactca
     6721 gatggatatc ttcatcgctg cgcagttgtt tcactttttc tcccaagaaa agatccccgc
     6781 tgaaaagcca cccctgctcc tcttccagca gacagatatg atcgaccgaa tgtccgggtg
     6841 tatggatgac cttaaagagg taatggcggc ttgataaata ttctcccaaa ggattgcagc
     6901 gggatgccgg gggataatcc cataccagtt tttgataatc aagcaaacgg tccgtccaga
     6961 gagatggatt ttcgatcagc gcaactgctt tatcatgagc aaaaatcggt attccttgct
     7021 tgtccagaag ataattgttg ccgatatgat cctcgtgatg atgtgtattg atgacctttt
     7081 cgaccactag ggaagggaaa atttttgccg cctcgtcagt cacagaataa gggccggtat
     7141 caaataaaat cccatccaca taataagctg ccatgctgta taacacttgt ccattgactt
     7201 ccttgcccat ttccaattgc attacgttgc caaacggaga catcttgatc aagccgatcc
     7261 ctacttcccg tcagataatt tgccaagcaa gcttcctttc ccttcgtcta tctatgattg
     7321 taccataata gcatcacgct ggtataaaga agtgaacaac ctgagaattt tccctgtcgg
     7381 tgaggggctt ggttcgtgtt atgatgttta atagagggca taaacatttg taagtttgaa
     7441 taatgctctg ttgagacaat acaattatat attcaacaag aaagaggggt tgacatgttt
     7501 atagatcaaa taaaggaaat cgcaggtcag aaaatccatc atcctggagt aatggaaaac
     7561 gtgataaaaa aggttttaat tggtccgaag caaggctggg aaggttggac gatgcggcag
     7621 tttgacctgg ccgccaacgg ttttacgcca cggcataccc atccctggcc ccatatcaat
     7681 tatgtcatca gtggtcaggg cttattgtat ctggacggtc atgattatga agttggtccc
     7741 ggctcgatcg cttatgtacc agacaatgtg gaacatcaat ttaaaaacat cgggtcagaa
     7801 gacttttctt ttatctgcat tgtccccgag gagggggata aatagttggt ttcttaataa
     7861 tgatggtata aaattgttgc agggaaggaa aagctcattc aaatgtaata gattcttgtg
     7921 tggacgggag gataaaatga ggaagctgtt gacgatcttg ttggtcatgt tttttacagc
     7981 tggtttggct ggctgtggtg aaacgggaaa caacgcagct gaaatgggag aacataaaac
     8041 catcacgatt ggcgttatgc cggatgttga atccataccg tttattatcg cggagaaaaa
     8101 cgggtatttc tccaaagctg acgtccaggt caaaatcgaa cattttacca gtgccaagga
     8161 ccgggacagc gcgctgcaga ccggcaggct ggacggagtc attacggacg tgctggcagt
     8221 tgtgtttgcc aatgaaggtg gcattgatct taaaatgatt gccagaagtg aaggcaatat
     8281 tttgctaatg gccggaaggg aggccatcat cgcttctctg gatgatttgc ctggtaagtc
     8341 ggtcgggctt tccaccaata cgatcatgga atacactgtt gacaagatgc tggcaacaga
     8401 acaaataaag gcagaagaaa ttaataaaat tgccatccca ccgctgccga cacgtttgga
     8461 aatgctgcag ggcggcaaaa ttgatgcggc cattctgccg gaaccgttgg ccggtttggc
     8521 ccttaaagac ggggcggtgg tattgagcag taccgatcaa atgcaccata aagcgggagc
     8581 gattgctttt accggcaaaa gcttaaagga aaacccggat gaaattaagg ccatcttcgc
     8641 tgcctataac gaagcggtag attatctgca aaagcaaccg cgggagagcg atgttgaatt
     8701 tatcattcag gaacagggat ttccgtctgc catcaaggac agttttactt tgccgcatta
     8761 tacccacgcg gaagcgctgg atgccaggat tgtcgctgag gtagtgcagt ggatgctaag
     8821 caaaaattta atcaaacaaa gctatgctta cgaagacctc accgcagaag acattttaag
     8881 gtagtgaatt tgtgatagat attaaagatt taagcgttcg ttatggcagt ccggacaatg
     8941 cggtactggc gcttgaacaa atcaatataa atatcggcca gggagagatt tttactttta
     9001 taggtccttc cggttgcgga aaatcaacct ttttatatgt cttgtcagga attattcgag
     9061 attatgaagg aaccgtactg ataaacggca gaccggtaga tcccaggcaa caaaggattg
     9121 gtttgattct gcaaaactat ggcttgctgc cttggaaaaa tgtccgtcag aatattgttc
     9181 tgggagtcaa aatcaaaggc cagccacggg aagaagaata ctttgattat attctcaaac
     9241 aattagggat agaccaattg ctggatcgct atccgaaaga acttagcggc ggacagcagc
     9301 aacgggtagc aattgctcgg gcatttttgt taaaacctga tatattgctg atggacgaac
     9361 ctttttccgc gctggatgca atcagccgcg aggaaatgca ggaactcttt ctgaaaatat
     9421 ggcaggctca ccatttgact accgttttca tcacccacag tgtggatgag gctctttatc
     9481 tgggtagtaa aatcgtggtg ttttcatctt ctccgggcag gatcctgggg atttatgaaa
     9541 atccctgctt ccaacgagat aatttacgtt atcgcgatga gtattaccag atgtcaagac
     9601 aattgagaaa tttactggat aaaggatgag cacattgcag tcaagtgaaa agtggaaagg
     9661 cgcattgtac agctttgtgc tggtcatagt tgtctggcag gcagtggcca tgctgctcaa
     9721 aatgccgatc ataccaacac cgacagcggt atttgtcaat cttgtggagg tttttcaaat
     9781 tcgaatggag atccatcttt ggtatagtct gggcaggatt gtagcgggca ttgcgatttc
     9841 gattctgctg ggtgtttctc tgggtttgtt aatgggctat tttgccaaag tagacaagat
     9901 cttttcccca ttggtctatt ttacctaccc ggtaccgaaa attgcactgc tgccgattgt
     9961 gatgctgctg tttggattag gggaagcttc caaactgatt atgattgtcc taattattgt
    10021 gtttcagatt gttattactg cccgcgatgc ggtgaagaat ataccgcagg aaatgtttcg
    10081 ttctttacaa tcactgggag ccggtcgccg gcagatattc aaggaaatca tctttccggc
    10141 gtccctgccc gaagttctta ccgctacccg tctggcttta ggaactgctg tatcgatcct
    10201 gttttttaca gaaactttcg gtaccgaata cgggatgggc tattttataa tggatgcctg
    10261 gatgagggtg aattacctgg atatgtatgc cgggatcgta atgctaagtc tcttgggatt
    10321 ttgcttgttt accctgctgg acgtggccga gcaatacttt tgttcctggc gctaagcatt
    10381 cgtcggccgc aaatcctagg gtttggggag gttgtttcat gccgattgcc accaggagag
    10441 ccaagggcaa tgcacaagtc atcattgacc atgagcattg cagtctttgc ggtctttgtg
    10501 tagaggtttg caaagggaaa cctttgttta ttcagggtga tcgggtttgt gttgatcaaa
    10561 gcatcttgtt tggttgtatt ggctgtggtc agtgtgctgc agtgtgtcca caacgttgca
    10621 taattatcga aggacgggat ctttcggcag aagatctgct ggagatgccg cctcgtgaac
    10681 aatgggctga ttacactcaa ctccataccc ttttacaggg tcggcgcagt accagaaatt
    10741 ttcagcgccg ggaagtggaa agagaggtca ttgaaaagat tcttgatacc gtggctactg
    10801 ctccgatggg acttccgcct tcggaagtgg aaatcctggt tttgcatggg tttaatcggg
    10861 tccgggagtt ctcccaagat attgtgggtg ttttcgaaac ccaaaagtgg atgttttccc
    10921 cgccggcctt agcgctgatg cgacctttta tcggcaagga tgtgtatgga ttgatgaaaa
    10981 ccttcgtcgc gcctttgccg aaatttttca gtgaaaagat ggcacggggt gaagactggc
    11041 tcttgtatga tgcgcctctg gccatgtatt ttcatgcttc gccgacggca gatcccatgg
    11101 attatgcgat tgctgcaacc tatgccatgc tggcggcaca atcgttgggg ctgggaactt
    11161 gcatgatcgg ctcagtcgca ccctttttaa aatataaaaa caaaatcaac aataaatatg
    11221 gcattccgaa agacaatcag caaggtttga tggtgatatt cggatatccg caatacaaat
    11281 atacccgcag catcaaaaga agtctgggca acgtaaagtt ttatgaaggc taaccggcat
    11341 ggggcgcatt atcgggttag atatattaaa aaagggagaa attacagatg aaagctttta
    11401 aaaaagtcct gatcgccaat cgaggagaaa tagccattcg aatcatcaga gcctgtcatg
    11461 aattaggcat gagaaccgta tcggtatact cgaaagagga tcaattagcc ctgttcagga
    11521 caaaatccga tgagtcatat ctgattggca acaacaaagg cccggttgaa gcttatctgg
    11581 ccattgaaga aattatcaac ctggcgatta aaaaaggggt ggatgcgatc catcccgggt
    11641 acggattttt agccgaaaat cccgaatttg cgaagaaatg tatcgaatcc ggcattgaat
    11701 ttatcgggcc taccccggaa atgatggata aactgggcga caagatcaaa tccaaattga
    11761 ttgcccagga aattggtgtg ccgaccattc cgggggtgga gaaacccatt aaatcagacc
    11821 gggaggccat cagatttgcc aaacaatgcg gctatccgat catgttaaag gcatctgccg
    11881 gcggcggcgg acggggcatg agaattgtcc gcagcgagga agacttgctc agagagttcc
    11941 acagtgccca aaacgaagcc cgcaaggctt ttggcagtga tgaaattttt attgaaaaat
    12001 acctggatag tcccaaacat atagaagtac aggtgttggg tgacaattat ggaaacatcg
    12061 ttcacctttt tgaaagagac tgttcaatcc agagacgtca tcagaaggtg atcgaattta
    12121 cccctgccat cgggatttct gaaacccagc ggcaggcgat ttgtgcagat gccataaaag
    12181 tcgcccagtc agtaaactac agaagcgcag ggacggttga gttcctggta gacaatcagg
    12241 gacagcatta ttttattgaa atgaatccgc gcatccaagt cgaacatacc gttactgaaa
    12301 tggtgaccgg gattgatatt gtacaaagtc agatcttgat tgcagaagga catcgtttgg
    12361 attcacaagt cgtaggaatc ccggaccaaa gtgcaattca actgcgagga tatgccatcc
    12421 aatgccgggt caccacagaa gatcccaaca atagtttcgc cccggatacc ggcaagttgg
    12481 atgtgtaccg aaccggatca gggtttggga tcagactgga cggtggcaac ggttataccg
    12541 gttcagtcat cagtccttac tatgacagtc tgctggtaaa aatcattgcg cattccagaa
    12601 cttttgaaga taccattaaa aaagcccagc gttccatccg tgaaaccgtg atcagcggcg
    12661 ttaaaaccaa cgaagcattt ttgctcaacg tgctttccca tcctaccttt gtgcgaggag
    12721 aatgtgatac cggtttcctc gatgcaaccc cagaaatctt tgacattact cccaaggctg
    12781 accgtgaagc ttctattttg caatacatca gcgaaaaagt tgtcaatgag ggcaagggga
    12841 tcaggaaaga ttacgatgtg ccaaagattc ccaagttcaa cagtgaggaa gtactggttg
    12901 gcagcaaaca gatattggat tcccggggtc cccatggggt ggttgagtgg atcaaagcgc
    12961 aaaacaaact gctcatgacc gataccagca tgcgtgatgc ccatcaatcc ttgatggcta
    13021 cccgcatcag aacggttgac atgttgcgta ttgcggaagc tactgctcat ctgggccagg
    13081 atctgttttc cctggaaatg tggggcgggg cgacttatga cgtagcgtac cgtttcttaa
    13141 aagaaagtcc ctgggacaga cttacccagc tgcgggaaaa aatacccaat attatgtttc
    13201 agatgttgtt gcggggagcc aatgcggtcg gttacaccaa ctatcccgac aatgtgatcc
    13261 gggaatttat ccaggagtct gccaatgccg gcatagatat tttcaggata tttgattccc
    13321 tgaactggct gaaaggcatg gagattgccg ttgatgaaac cttgaaaagc ggtaagatag
    13381 ccgaagcatg tatctgttac accggtgata tactggatga cagccgcgat aaatattcac
    13441 ttcgttatta tgttgaaacc gccaaagaga ttgaaaaaat gggcgcacat atcctgggca
    13501 tcaaagatat ggccggtttg ctaaaaccct acgcggcgga aaaactaatt aaagcattaa
    13561 aagatgaaat atccattcca atccatctgc atacgcatga taccagcggt aatggtgtcg
    13621 ctaccttgtt gatggcggca gaagcagggg ttgatattgt tgatactgcc tttaacagcc
    13681 tgtcagggct gaccagtcaa ccggccctca attcggtggt agcagcgttg gagaatacca
    13741 accgcgatac cggcatcaat cttgacgata ttcaggaaat ctctgattat tgggcggatg
    13801 taaggcctta ctacagcgaa tttgaatcag gactaaaatc cggcactgct gatatttaca
    13861 agtatgagat tcccggcggc cagtattcca atttgaagcc ccaggtggat agctttggac
    13921 tggcgcacaa attcaaagaa gtcaaagaaa tgtataaaac cgtcaatgaa atgatggggg
    13981 atattgtcaa ggtgacccct tcctccaaaa tggtcgggga tatggccata tttatggtga
    14041 aaaacgattt aaatccaaaa aacatttatg acaaaggcca agatttggct ttccctgatt
    14101 cggcggtagc ttatttccaa ggcatgatgg gacaaccggt gggagggttc cctcaggagt
    14161 tacagaagtt ggtgctgaag ggcatcgaac cgattacctg ccgaccggga gaattgcttg
    14221 aaccggtaga tttttatgcc agcagcaaat atctggagga aagatacggt tttcaaccaa
    14281 accgcagagc tttgttaagt tatgcgctgt atcccaaagt atatgaggag tatattgagt
    14341 ttaaaaaaca gtatggtgac ttgagtcgta tgaacagccc ggtatttttt aacggcatca
    14401 gtgaaggcga agtttgtgaa gtcgaagtcg aggaaggcaa gacctttatc attaaactgg
    14461 tggcgattgg gaaaatcgac aaagaaggct accggaagat ttattttgaa gtaaacggca
    14521 atcaaagagc aattaacatc ctggataaat cttaccagaa aggcgaggct gcgactgcca
    14581 cggtcatggc tgaccccgat aacaaaaatg aaattggcgc cagtatacct ggaacagtgg
    14641 ccagaatact ggttaaggaa ggggagcaga tcaaaccagg gcaaaacttg attgtgatcg
    14701 aagccatgaa aatggaaacc caaatcatcg ctccggtagg cggagaagtc acgtttatca
    14761 atgttaccga aggccggcag gttaaaaatg gggagttgtt gttgaagatc gagtagcaga
    14821 taataagaaa agctcccccg caataaatct ggccggggga gcttttttgt gggcgttcag
    14881 atggttggga agatactgcc gatgatcgct ttgatggtgt catccgggat cggatacagc
    14941 atggctgtgg ggtcggcttt aatcgcccaa aacagggttt cctgctgact tgcgtccaag
    15001 gtattttcaa aataaggctt aaagccacac tccttcatca gtgacaatac tttttcccgg
    15061 gccagtgcga ccagttcagc cggatccggg ctgtccgatc tgaccccgaa cccttcggcc
    15121 aacgctttgg ctttgggcca gtaatgccaa ggcatatgct ccatacaggc aggcaaggcg
    15181 accccaactg cctggccatg cggaatacga agctgtccgc cggcggcatg ggcgatatta
    15241 tggacgggga acatcatgcc acccatggcg aatcccataa tcgccatatt gctggcagcc
    15301 agcatattgg tacgggcttc gaggttctta ccgtcgctgg cggcaatcgg caggtattgc
    15361 atgatcattt tgatggagtg gatgttgagc gaatccgcca taatattcgc ctcaggtgat
    15421 gtcagtcctt cgactgcatg gccaagcgca tcaagtccgg tggaaacagt catagccggc
    15481 ggcaaaccaa ccgtcaggtc aggatcgagc agggcgatgt cagccggcac atagggatgc
    15541 aggacatccc ctttgacctt gtctgcttcg ttgtaaatga ccgccagagg agatacttcg
    15601 gatcccgtgc cggcagttgt ggggataaaa atattaggta cgccaagagg ttttcccaag
    15661 ggacggatgt agggccccag attgccgggc attaagtttt taatgtcggt tgcgccgcca
    15721 cccagaagca tttttacgcc tttggctgaa tccattacac tgcccccgcc tacaaccagc
    15781 ataccgtcaa aggcattttc ccggtaccag cgggcacagt cattgatgac ccgcatggca
    15841 gcgtcctgtt caattttatc ataaaccccg gccatcttaa cgcccccttg aatcgcaaac
    15901 acatctttga ccatgtcggc tactccagca tcgatcaagc ctttgtcggt aatcatgcca
    15961 acccgtttac aacccatttc cataaagcgc tgggctgcaa acgtgcggca tcctgagccg
    16021 acttccacca gagtccggta ttcccaaacg aaatattttg gtgttgccat tacagaaacc
    16081 ccctttctat cggttaaatc ccaggaaagt ataaatgaaa ctgctcttgg catcaggggt
    16141 gagatcctga cagatgtgtt tcacttcgct gtaagccatc aaagcatggt aacaattttc
    16201 tcgcccataa ccgctttctt tgtatccacc aaaaggtaca tcaggacgca ggatatgcca
    16261 ggtattgatc cagacactgc cggtgcgcat acgcttggcc accttttgcg cccgggtggt
    16321 attggtggaa caaacacctc caccgaggcc gtaagggctg tcattggcga tggcgatcgc
    16381 ttcatccaga tcatcataag ggatcacaca ttgtaccggc ccgaaaattt cttcccggac
    16441 gtgctgcatt tggttgctgc aattgataaa aatggtaggt tcatggtaga agccattgtc
    16501 atagataccc ccgctcaagc ggttgccgcc gtaagcgatt tgggcacctt ccttctgtcc
    16561 gatctttaca taattcataa tcgtctgcaa ttgctgggag gaagcgatgg gtcccaagtc
    16621 ggtagccaga tccatctggt cgccgatatt gagttttttg attttggtga tgatggcatc
    16681 aaccagttga tcctgcatct tacgagggac aatcagacgg gtgccggact cgcagacctg
    16741 cccgctgtgg gtgaggaaag ccagcaaggt catgctggag gccatttcaa tgtcggcatc
    16801 atcaagaacg atcaacggtg atttgccgcc caattcaagg gttacacgct tgatgccgtc
    16861 ggcagccaga tgtgcgattt gtcgtcctac ctcggttgaa ccggtgaaac cgattttatc
    16921 cacttgggga tgagcagcga ggtagttgcc gatcgatgct cccggaccgg ttacaacgtt
    16981 gaatacccct ggggggatgc cggcatcgga caaaatcttc gctagttcca gcgtggtgac
    17041 cggagtaata ctggctggtt tcatcacaat gctgttaccc atggccagcg ccggggcaaa
    17101 tttatatgcg gccaggatca tggggaaatt ccagggggta atacctgcac atacaccaat
    17161 tggctcacgt atggcatagc tgtgcatagg ggctacccaa ggcgggcaaa tagcatgttc
    17221 gacctccggc atttgtttga ggatggcggc tgacatggcc aacgaaccgg caacggacat
    17281 aacatcaacg aatccggtcc ggcgtaatgt ggcacctgag gtaagtgctt ccagccaggc
    17341 caatcgatcc ttgttggcga ggactgccat ggttgcgcgg gtcagcactt cgattctttc
    17401 atcgatggat ttgcctgacc aaataccgga ttcgaaagca gctcgggctg agtgcagggc
    17461 tttgtcaata tcaccttgtg ttgcccgcgg tacacgagcc acaacctcct gattggcagg
    17521 attgatgaca tccatcatgt cgcctgattc accggctgtc cattgcccat caatgtacaa
    17581 ttggtattcc agtatttcct tacttaccaa caaaacctcc ccctctcaaa tttgtgattt
    17641 agcgatcata atatcatatt aacatagcat tatatgaaat aaatactaat taaggtaaat
    17701 gatgaatata catataatca tcctcagatt ttatagacat aaaaaaagac ccaccgcaat
    17761 gatgggcctt accattatta atgctactcg attttattgg tccggcgcca gatcttgcgc
    17821 tcttccgctg ctttaatcaa atcatcacgg aagtcggggt gagcgatgga gatgagtttc
    17881 tccgcccggt accaggtcgg acatgttttc aggctgatta cgccgtattc tgtgacgaca
    17941 tgattgatca tctggcgaga aagggtagtg atggagccgg gttcaaaatt gggaacgatc
    18001 ctggaaataa cggtgccgtc tttctttgta tgggtggagg gcagacaaat aaaactgcga
    18061 ccgcctttgg accagaatgc accgagcacg aagtcagaca tgccgccatt tccagatacc
    18121 tgtttggatc ctgaactttc agcattgacc tggccatata agtcaacctg cagagcctgg
    18181 ttgatcgaga tcaggttgtc aatctgtgcc agttgcatgg gatgattaac ataaccgaca
    18241 ctgcaggagg caatagcggg attgcggtcc atccaatcat agagttcctt gccgcccaga
    18301 gcgaatgtat aattaatttt cccgggatcg aaaatctttt taataccggt cattctcccg
    18361 gcttcccaca tatgcatata tgcatcgacc agcatttctg tccagccgct cagatcctta
    18421 agatcggttt cgctgatcat ggttcccagt aggttcggca tagccccgat tcccaactgg
    18481 atgcactgac cgtcctgcaa gtattccaaa acattggctg cgatttttgc atctacatcg
    18541 gttggatcaa ttttggccaa ctcatacatt ggcgggtttt ctccctctac gacgtacgtt
    18601 acctgggaga tgtgaatccg ctccatattt ccgccatagc agtaaggaat attctggtta
    18661 acttcgacaa tacgattctt agcggttacg gcctgcaaat agcagcatga attgtgcagt
    18721 ccccagttga aatagccatt ctcgtccata ggcgtagttt gagcaataaa tacgtcccgc
    18781 agattggtgc cgatcttgct ttttttagag cccgggtctt cttcataatt gccgtatttc
    18841 atatacattt ctgattcacc atatagtgtg ggctgataga aaacatttcc gcatctttcc
    18901 tggaaaatac gggtcaaggc actgaaatgt tgatcgttgt aggtgaaaac ctctcccatc
    18961 gggtctttca tgacaacttc cggaagcggg ggcacggtta ctgcacccat aacattgata
    19021 tttttcaatt cgtccttgcg tgctgccagg gctgtgtcac aagctaccgg tttgccatta
    19081 aacatggcgt aatcaatcca tgctccggat tttacaactt tcattgcctc ttcggctgta
    19141 cgtaatttgt ccttgtacat ttgatgaaaa tctttgttca caaacaaatc cccctccgca
    19201 atctcaattt gtaataatca tttcaacaat attacattga attatatatt aaaacaaaat
    19261 gaaataatag gtatttttac gaagtaataa aacacgcaat attacgaaaa ttgggaaaat
    19321 aaacacaaat atagaattga ttttagaaat gaaatggcat gttcattgca ttataattaa
    19381 atctatggat gtgtatgtaa caaaaggagg aagcgcaatg aaggtattgg aaatgtttaa
    19441 attaaacggc aaaacagcgg tggtaacagg aggatcaatt ggtttaggag cctacatggc
    19501 aaccgctttg gcggaagctg gagccaatgt ggtaatcgct gctcgcaaag ttgaccgttg
    19561 catcgaattg gctgaagaat tggaaaaatt aggagttaaa acgctgggtg tagcctgcga
    19621 tgtaagcaaa gctgaggact gccagaattt agtggacgtc actgtgaaag aattcggcag
    19681 ccttgatata ttggtcaata atgccggact gacttggggt ggagattcga tgacctttgc
    19741 catgaaagac tggcacaagg ttatggatct caacctgcat ggattgtttc aattgacgca
    19801 aatggccggt cgggtaatgc gtgaacaggg ttacgggaag atcatcaaca tctgttccct
    19861 gggtggatat ggcggaacca aaccggaaga aatggatgcc gtagcctaca atgccagcaa
    19921 aggggcattg atgacgttaa ccaaagacct ggcggtaaaa tgggccagat atggaatata
    19981 tgtaaatgcc cttgctccgg gattcttccc cacgcatatg acggaatgga ttctggaaaa
    20041 gaattcaaaa tacctcctgc ccagagttcc tttcggtcgt tacggcggag aagacgatct
    20101 caaaggagct gtggtgttcc tggcctcggc agcttcaaac ttcatcactg gccagatcat
    20161 caatgttgac ggcggtgaaa tggccatggt tccctagaga acaaatacgg tttatttaat
    20221 ttacgaaaac ccggaaatat tccgggtttt tctattgtgc ttttggttca tgattaaacg
    20281 atgatggcgc aattgtgcca aatggaatca atatgggcag agtggttgac attctgtatc
    20341 aatttttgct gggttatctt acctttgacg cttcttctgc gatgcctata aaatgataga
    20401 tatccaaatg aattatcaaa caatcgtggg agcaaatttt aatcctggaa aaaggaggat
    20461 gaaaaacagg taatctatat ggcaaccatg ttgttcattt gatgcaatct attttacaag
    20521 gagaaaaata tgaaccataa gaccgcagca agtattttgg cagttttact gtttttcggc
    20581 gtatgcattt acatatcagg tatcataaaa ccgcttgaca aactcgctgg gagcgagcgc
    20641 acaaatgaga ttaaaacgac tcaaaacagc ttggaacagc gcgcttcagt tgataaggaa
    20701 gtcacagtga agcgtactga tagccagaaa aaaacaaaaa aagttcagtc tcctgcccca
    20761 ccggcagagc cgaaaatcgt tcaagtacaa acagaaaaca gcgtgcagga taaaagcagc
    20821 aatccatcgt ccgggacgac cagcgtgaat cagcatgaaa gcagcgcggc aagcaaccct
    20881 gccggctccg gcagcaaccc ggtaagccag ggtaacagca ccaattccag caacaatcaa
    20941 gtatcgcgcg ggga
//
LOCUS       DAOTHZ010000037        19877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3279_length_19877_cov_7.580517, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000037 DAOTHZ010000000
VERSION     DAOTHZ010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 19877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19877
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3279_length_19877_cov_7.580517"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1389
                     /locus_tag="P5549_05880"
     CDS             211..1389
                     /locus_tag="P5549_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRW12636.1"
                     /translation="MKINDSSKRMIVLFATIFIVGVGFSIIMPVLPYYAESMGANAFQ
                     LGLLITVYAICQFIFAPIWGAYSDRVGRRPLLLLGILGYAVTFLLFGFATQLWELYVI
                     RIIGGILACSTMPTAMAYVADLTSLEKRGSAMGMIGAAMGMGMVFGPAIGGILSSISL
                     AFPFVFAGLLALVNAVLLYILLPESLASENRIIRKVERASLLEGLRSPLVVLFLFICL
                     GSISESIHHGTFALFAEQKLSFTARDIGWAFTAAGIVMALIQGVLVGRLINRFGEEKT
                     AAAGIILMGTSFGLFLLMYDILSSIAFMAIFSAGVGLMRPSISAAVSKRTADGQGSAM
                     GILNGYDSLGRAIGPMLGGFMLDLGLNYGYYTAIVVCLLALLTLSLSRQLKGRRAPQV
                     "
     gene            1656..2417
                     /locus_tag="P5549_05885"
     CDS             1656..2417
                     /locus_tag="P5549_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRW12637.1"
                     /translation="MDYTVHKLARLAGVSTRTLRYYDEIGLLIPPRMNSSGYRYYGQA
                     EVDKLQQIMFYRELGLDLQTIKEILDAPDYNRLAALAEHRDKLLMKRKQLELLIANVE
                     KTIAAQEGRCIMSDREKFEGFKQKLIDDNESKYGKEIRKKYGTDTVDASYQKLKNMTP
                     QQYEEMQKLTDQVNEALRLAVADGYPGGKLAMQACDLHRQWLMQSWPQYSKEAHMGLA
                     QMYVDDERFAAYYDQIAPGCAVFLRDAMKVYTGME"
     gene            complement(2484..3131)
                     /locus_tag="P5549_05890"
     CDS             complement(2484..3131)
                     /locus_tag="P5549_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12638.1"
                     /translation="MKKDLTELVFILDRSGSMSGLESDTIGGYNAMLKKQQQEPGEAV
                     VTTVLFDDQYELLHDRINLKGIRPISEKEYYVRGCTALLDAIGKTIQKIANVQKNTSE
                     EERAEKVLLVITTDGMENASQEYRYKQIRSLVEQQQKKYGWEFIFLGANIDAIAAAAD
                     LGINADRAANYHADSAGTQLNYDAVNDAVSDLRKTRSIKTNWKARIDEDYQRRGE"
     gene            complement(3211..4212)
                     /locus_tag="P5549_05895"
     CDS             complement(3211..4212)
                     /locus_tag="P5549_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRW12639.1"
                     /translation="MPLQIVRQDITKMEVDAVVNAANQALKMGGGVCGAIFTAAGAQQ
                     LQTECDRIGKCQTGSAVITGAGKLPAQYIIHTAGPIWKGGQSDEARLLHDCYIHSLEL
                     ARQHDCESIAFPLISSGIYGYPKDQALQIAVAAISEFLLQHEMMVYLVVYDRQSLALS
                     EKLFTALEKFIDDHYVEKLQKKFSANRRLQHYELLEEVQVDYNQMQADLAPRKLDELV
                     NHLDETFSRMLLRLIDEKGQDDVETYKRANIDRKLFSKIKSNPSYQPSKLTALAFAIA
                     LQLSLDETRDLLGRAGYALSRSNKSDVVIEYFIQSGNYNIFEINQALFHYELPLLGA"
     gene            4380..5354
                     /locus_tag="P5549_05900"
     CDS             4380..5354
                     /locus_tag="P5549_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRW12640.1"
                     /translation="MRIETVSIIGLGALGILFGNHLAGQMPAENLRIIADRERIARYT
                     REGIFCNGQPCHFHYVTPEEQGPPANLIIFAVKYGGLNEAISAVRHQVGKHTVLLSLL
                     NGISSEESIAAAYGTERVLFCVAQGMDAVKVGNQMTYANMGMICFGDRQPGIISAQAQ
                     AVADFFTAMQLPHQLDTDMNRRMWGKLMLNVGVNQAVALLGSAYGDVQREGPAREVMV
                     AAMREVIALSQKENVNLTENDLQYWLQVVSTLSPLGKPSMRQDIEAGRASELELFAGT
                     IIRLGEKHHLPTPVNQMLYDGIRSLENGFQPSAPDKESPSGSSASTDK"
     gene            5502..6365
                     /gene="metF"
                     /locus_tag="P5549_05905"
     CDS             5502..6365
                     /gene="metF"
                     /locus_tag="P5549_05905"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRW12641.1"
                     /translation="MKTSDHFKNKTVLSLEVFPPRRTAPIHLIYETLEGLQGLSPDFI
                     SVTYGAGGSEMNTATLEIASAIKNHYGIESVAHLPCINLTRSEVLDLLDGFRQAGIEN
                     ILALRGDINPDFLPQQDFRYASELVSFIKENGDFNVIGACYPEGHSESDSLAEDIRHL
                     KNKVYAGTDQLITQLFFDNDFFYSFRERARQAGINVPIQAGIMPVVNKKQIERMVTLC
                     GVKLPRKFVTMMGKYEHNPVAMRDAGIAYAVDQIVDLMAEGVDGIHLYTMNNPLIAQK
                     IYAAIRSLLAA"
     gene            complement(6375..7295)
                     /locus_tag="P5549_05910"
     CDS             complement(6375..7295)
                     /locus_tag="P5549_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRW12642.1"
                     /translation="MTLQQLRYIIKIVECGSISEAAKQLYIAQPSLSNAVRELEKELG
                     MEIFYRTSKGISLSVDGSEFLAYARQVVEQMELLEQRYLGKKPSKQLCSVSTQHYAFA
                     VNAFVNLLRGLDVDEYEFTLRETRTWEIIEDVKNLRSEIGILYFSDFNEKVLNKLLKE
                     NHLLFHPLFAAQPHVFISSSHPLAKRKSVTLEDLDDYPILAFEQGEYNSFYFSEEILS
                     TVPHRKTIHVSDRATLFNLLIGLNGYTISTGVLNSDLNGDNIVSVPLQTEERMLVGWI
                     ASEKAHLSTIATDYIAELRRVIADYGHTVL"
     gene            complement(7571..8932)
                     /locus_tag="P5549_05915"
     CDS             complement(7571..8932)
                     /locus_tag="P5549_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12643.1"
                     /translation="MNWQEKYKKKLITADEAARLVKSGDWVEYGFGINCARDFDEALA
                     RRVDELTDVKIRCDIGAYQHFTAEADPQGAHFLWNSWHVSGQDKKFIGKNLFYIPMKF
                     HENPMMTRKDCVPDDVFVMQVTTMDKHGYFNFGGSAVNGWAAMETARITILEVNDKMP
                     RCLGGNQENVHISQVDYIIESKNQPIVTLGSSEPSQAEVEIARHIINRLYNGNCIQLG
                     IGGTPNAVGKMVAASDLKNLGVHTEMYVDAYLEMFKAGKITGSRKNIDRYKQVYSFVM
                     GSQELYDYVDDNPGLASYSVDYTNDPKVIAQIDDFVSINACIEVDLFGQVCSETVGTR
                     HISGTGGQLDFVEGAYKSQNGQSFICLPSTIEIKGQVSSRIRPILTPGAIVTSPRTAT
                     HMLVTEYGIADMKGKSTWERAEALINIAHPDFRDMLVQEAEKMNIWRRTNQIEAAATA
                     VNF"
     gene            complement(9165..10142)
                     /locus_tag="P5549_05920"
     CDS             complement(9165..10142)
                     /locus_tag="P5549_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12644.1"
                     /translation="MIDQVLPGIYRIEIPLPNNPLKATNAYIIRGEKRNLLVDTGFNT
                     EVSRQAMDRGLQELGVKMENTDLFITHLHSDHSGLVKHLAAPDTMVWMSEADGNVVEA
                     SRDSSHWSMFNGFLIVSGLVDSGVENDFTKHPGYRFASEVYQHFNLIEEGFEIAAGDY
                     RFQCVATPGHTPGHMCLYDQGKKLLLSGDHILGKITPNITAWNFDTDVLGQYLQSLDK
                     IGQLEIDTVLPGHRYVITDCQGRIEELKRHHHSRLDNILDILGHQTMNTTQVASRMRW
                     DLSFKKWEDFPWGQKLFASGEAMSHLHHLTAEGELEMRLENKIAWFRKK"
     gene            10330..11865
                     /gene="cls"
                     /locus_tag="P5549_05925"
     CDS             10330..11865
                     /gene="cls"
                     /locus_tag="P5549_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRW12645.1"
                     /translation="MQRLIKSLFSRVFLFGLAIIIQLFVLILVIWRFSNYFVFFYAIL
                     AVASLIAVLVILNGRSNPTYKLAWIVPILVFPIFGGLFYLMFGGSKSSRRNKRKMLVI
                     GLKMEDYLRQEPDVLRELEQDNPKAANQARYIDSYSFCPVYTHTTATYLTPGEIKFQR
                     LLEELRKAERYIFLEYFIIEEGIMWNAVLEILVEKARQGVDVRVIYDDVGCLMKLPYG
                     YDQKLERLGIKCSIFNPFRPLLSVRLNNRDHRKIAVIDGHTAFTGGINLADEYINAYE
                     RFGHWKDASIEIKGDAVWNFVVMFLSLWEWLRGGNEDYERFRPWREHTRNFASDGFVQ
                     PFSDSPFDDEALGESVYLNLINQAERYVYINTPYLILDNEMQTALRLAAKRGVDVRIV
                     TPHIPDKWYVHAVTRSNYEILIETGVRIYEYTPGFMHSKTVVVDDQLAVVSTINLDYR
                     SLFLHFECGVWLYKNNTIQEVREDFLQTLRVCQHITSEDCRHVKWYHRVIRSFLRMFA
                     PLM"
     gene            complement(11876..12412)
                     /locus_tag="P5549_05930"
     CDS             complement(11876..12412)
                     /locus_tag="P5549_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HRW12646.1"
                     /translation="MTPTKKLAIIAMLVAQAAVLHFLESLLPNPIPIPGVKLGLANII
                     TLLALLLFDFKTAMQITVLRTILGSLLSGTLFGVGFLMSFSGAIVSTIVMGLLVYIGR
                     GSAIAAVSVAGAAAHNLGQLVMAALILQFAGIIYYLPFMLLFSVPTGIITGLLVKTLV
                     EHTRAMDRSEILKDPARL"
     gene            12531..13328
                     /locus_tag="P5549_05935"
     CDS             12531..13328
                     /locus_tag="P5549_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRW12647.1"
                     /translation="MTGIKLGQYVHGDSLLHRLDPRTKIICCTLIVVAIIINDRWYSL
                     LFFALLMAAAIRASGMDIRFVLGSLRSIRSLLLVTFVFMAVLTPGQAVWTWGVLHVTR
                     EGLTMGAVNLLRLIILYLGSMVLLMTTSPMQLSAGIDWLLLPLSRLKLPVHHFSTILS
                     ISFRFIPTLIDQAVLIQDAQRSRGAQFASPRLGIRLKSYLAILIPLFEASLLRAEDLG
                     EAMDSRCFTGHPNQLRLDRLHFHAADGYVLISMLGLLAAGILFPVLL"
     gene            13345..14178
                     /locus_tag="P5549_05940"
     CDS             13345..14178
                     /locus_tag="P5549_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW12648.1"
                     /translation="MLEAGIVLQNVSKIYRSHNGDRCTLEDINLSIRPGEYVGLMGMN
                     GSGKSTLARLCNGLLLPTAGKVYVDGLDTSQAENLPAIRQRVGMVFQNPDNQLICPVV
                     EEEIAFGLENLGLFLPEIRERTAWALEIMGLTGLEYHAPHLLSGGQKQKVALASVLAM
                     KPAYLVLDEPAAMLDPVSRRELLQHLKILNRREGMTIVLCSNEAEDFMDADRLLVIDR
                     GTLCLQGTPPEVLSQGEQLASIGLAQPDFYRLRQQLADAGCSVTAIYNLTDLVDYLCH
                     P"
     gene            14166..15014
                     /locus_tag="P5549_05945"
     CDS             14166..15014
                     /locus_tag="P5549_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRW12649.1"
                     /translation="MSSIIEVKQLHYTYLPGTPYERQALKNISIAVEAGDFVGIFGPN
                     GSGKSTLARQFNGLLRPTQGQVLVCGKDTTNEKIRRSLWNKVGLVFQFPEQQIFQTTV
                     YNEIAYGPRNLGLPEEEIRERVGQALAMVGLATQDIANLSPFSLSGGLKRRVAIAGLL
                     ALHPEILILDEPMAGLDPVGRRMIMEIIRQRRENNATTLMISHDLQEILNVADKIVIL
                     DEGVLVFFGKTSELLDHPDILQSYRLQLPLYLQVVYALQEKKQPVKQNIGSLEEAAAE
                     IIKLFP"
     gene            complement(15011..15358)
                     /locus_tag="P5549_05950"
     CDS             complement(15011..15358)
                     /locus_tag="P5549_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRW12650.1"
                     /translation="MRKKEWVLLVSLTILIVLVAGAIRYLQQANSGDSRVAVITQDGR
                     LVERIDLQRVREPRQITLPGKYHEVVEVEPGRIRFKQADCPDKICVNTGWLEDPGDVA
                     VCMPNRAVVRIED"
     gene            complement(15434..16060)
                     /locus_tag="P5549_05955"
     CDS             complement(15434..16060)
                     /locus_tag="P5549_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRW12651.1"
                     /translation="MPKREPLPSKKNHIYRERNGQQEVVVKQFTDSQRFCMEKEIGTL
                     LKDSGLLTPRRIDINTRQQQIIYEYIPAMALVDLIETAEWEQVQAMMIQVCDWMMKFY
                     QTMREKTGQQYILGDIHLRNFIYHETSGQVYGVDFEECRPGRIESDVARLLTFLLHYE
                     PAFTARKIELADGLQKYFFAALDLDRDFYLQEVHRETEELMQRRHKNR"
     gene            complement(16047..16586)
                     /locus_tag="P5549_05960"
     CDS             complement(16047..16586)
                     /locus_tag="P5549_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRW12652.1"
                     /translation="MKVFSIVGISRSGKTTVAEAIIAELRRRNYRVGSVKDIHFEGFA
                     LDQEGTNTHRHKMAGAELVTARGLYETDVMFLSRLTITEVLRFYDQDFVILEGAYDFK
                     GPGLIAAVEENEIDERRWDKVIAVTGRIAGRLQEYQGLPVINALTEVVRLTDLIEQTV
                     SFWEEQPDWIGEDAHHAQA"
     gene            complement(16642..17391)
                     /locus_tag="P5549_05965"
     CDS             complement(16642..17391)
                     /locus_tag="P5549_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRW12653.1"
                     /translation="MTSVSDLDPRTKLILIMCLTSAAVLISNWIFLSGLCLVSFGLLS
                     CFGVSLWSIIKRIRLLLYMMIVIALLQSVFAPAGQTLLAVGSVKLVTAGGLLKGAEFL
                     LRMLIIITSAGILTTSNHREIIQGLVQWKLPYEIAFMASMGIRFLPVFAEEFRDAMIA
                     VQLRGVDIKKLPLRQKLEVTASLFQPVVAGAILKSKDIAMSIEMRGFRAYPTRTSYLV
                     LELKLRDYVIMLSAIVVTVILIGGYYVCFIR"
     gene            complement(17375..17986)
                     /locus_tag="P5549_05970"
     CDS             complement(17375..17986)
                     /locus_tag="P5549_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12654.1"
                     /translation="MIDQLNLCLYKGETVALIGPNGAGKTTLGKLLTGILKPAAGQLL
                     LFGEDARSLPLSLIGEKVGYCFQNPRQQLLAATVEEEIAFGLVYRGVRRELIDATVNY
                     LLELFEIEHLRHFFPLNLSWGEKRRVVLAACLALEPQYLVLDEPTVGLDAERIRTFNR
                     VLARLRDNGIGMLLISHDQDFVRDNAGRILPMKGGSIIDDLRV"
     gene            complement(18046..18702)
                     /locus_tag="P5549_05975"
     CDS             complement(18046..18702)
                     /locus_tag="P5549_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12655.1"
                     /translation="MRMLEAKSISFRYSGQPQMILENVTLSLEAGQITALTGPSGGGK
                     STLGYCLSGVIPTLIKGEFSGRITRQGRIGIVFQDADAQVFLPTVEDELAFGPENLCL
                     PRQEIEDRINGILELLNIEELRLKNPARLSGGQKQLVALGGVLTLAPDIIILDETLSQ
                     LDQTVRARVKRVLLALKRQGCTIIMIEHDSRIIDIADQIWSLAEGRLQQFAWTGGEQP
                     "
     gene            complement(18722..19249)
                     /locus_tag="P5549_05980"
     CDS             complement(18722..19249)
                     /locus_tag="P5549_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12656.1"
                     /translation="MNKNNRIIVIILAVLFITTTIGVIVNQRYAADRKALLEATEIAV
                     RESGQEVARLNMTVLGSMKTVEFTARLKSSLMEKPKEHTYTGIAFADLFTAAGISLEE
                     KQRVLVYSVDGYVVPLSIKEIKEFDNIYLVYKDNGNYLGSYEEADGQGPYMIVIRGDR
                     FSQRWAKYVCELDVQ"
     gene            complement(19287..19826)
                     /locus_tag="P5549_05985"
     CDS             complement(19287..19826)
                     /locus_tag="P5549_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRW12657.1"
                     /translation="MNNLLKRFGAFDLVIIALMAALGVAVKPIVTGLVHIITGPLIMP
                     GGSLAGGFYMLWLVLGAALVKKPGTAALIGLVQSIMVIVTGFYGTHGIVSLVTYTLPG
                     LTVDLLYWIARLKADEKMAMFAGCIGANLCGVLLSNLVFFRLPLIPLLFSLSAGALSG
                     ALGGLLAWLVAERFSKLNI"
BASE COUNT         4595 a         5126 c         5099 g         5057 t
ORIGIN      
        1 tgtatttttg ttattttcta ttatgtgttt ttcagtggtc tcggacggtt cttgcttgcg
       61 cgaacaaacc atgactgcgc gcaagcaaaa accttccaca cttgcgcaaa taccatagac
      121 agaacttcgg ctgtgcaagt cgcttgtttt ggcttggcgt aaggagtaaa ctggaataaa
      181 ttgtttttga acggtaggaa ttgtgaataa atgaaaatca atgacagtag caagcgtatg
      241 atcgtgttgt ttgccaccat cttcatcgta ggggtgggct ttagcatcat catgccggtg
      301 ctgccctatt atgccgagtc catgggggcc aatgcctttc agctggggct gctgatcacc
      361 gtctatgcga tctgccagtt tatctttgct cccatctggg gcgcttactc agaccgggtg
      421 ggcagacgcc ccctgctgct gcttgggatt ttgggctatg cggttacgtt tctcctcttc
      481 ggttttgcca cccagttatg ggagctgtat gtcatccgta ttatcggagg gatcctggcc
      541 tgttccacca tgccgaccgc catggcctat gtggcggatc tgaccagtct ggaaaaacgc
      601 ggctcggcca tggggatgat cggagcggct atgggcatgg gcatggtttt cgggccggcc
      661 atcggcggta tcctgtcgag catatcgctg gcctttccgt ttgtgtttgc cgggctgctg
      721 gccctggtta atgcggtgtt gctctatatc cttcttccgg agtctctggc cagtgaaaac
      781 cgaatcattc gcaaggtcga gcgggcttcc ctgctggagg gactgcggtc acctctggtg
      841 gtcctgttcc tgttcatctg cctgggcagc atcagcgagt ccatccacca cggaaccttt
      901 gctttgttcg cggagcaaaa gctttccttt accgcccggg acattggctg ggcctttacc
      961 gctgccggaa ttgtcatggc attgatacaa ggagtgctgg tgggccggct gatcaaccgt
     1021 tttggcgagg aaaaaaccgc tgctgccgga attattctga tgggcaccag ctttgggctg
     1081 tttttattaa tgtatgatat tctttcctcg attgctttta tggcgatctt ctctgccggg
     1141 gtaggtctga tgcgtccttc catttccgca gcggtttcca agcgaacggc tgacgggcag
     1201 ggttcggcca tgggtatttt aaacggctac gacagcctgg ggcgggccat cggcccaatg
     1261 ctgggaggct tcatgctgga tctgggtctg aattatggct actataccgc catcgtggtt
     1321 tgcctgctgg ctttgctgac gctgtcgctc agtcgtcagc tcaaaggacg ccgcgccccc
     1381 caggtttagc agtagggacg agaaagtttg gaaatataaa cgcccggagc cttctgtgac
     1441 ccgggcattt tttgtatcta ttgattcctc ttggccattt cctcgtcccg caggcgcatc
     1501 ttcctgaatc atttcaaagc gcggggtaaa ccggttatag gatatgtgtg attcaagatt
     1561 gtttcgtgaa gattacccgt tgccgtaaaa caaattttcc ctcttgactt tgacgttgcg
     1621 taaaggttta ggatgtattt aacggaggtg acctcatgga ttatacggtg cataaactag
     1681 ctcgactggc cggagtcagc accagaactt tacgttacta tgacgaaatc ggtctgttaa
     1741 ttccgccccg gatgaattct tccggctaca ggtactacgg tcaggctgag gttgataaac
     1801 tgcagcagat catgttttat cgggaactgg ggcttgatct gcagaccatc aaggagattc
     1861 ttgacgcgcc tgattataat cggctagccg cgctggccga acatcgagac aagctcttga
     1921 tgaaaaggaa gcaattggaa ttgctgattg ccaatgttga aaagacgatt gctgcgcagg
     1981 aaggaagatg cattatgtcg gatcgagaaa agttcgaggg ttttaaacaa aagcttattg
     2041 acgacaatga aagcaagtac gggaaagaaa ttcgaaaaaa gtatggcaca gacacagttg
     2101 atgcttcata tcagaaacta aaaaacatga ctccacaaca atacgaggaa atgcagaaat
     2161 tgaccgatca ggtcaatgag gccctgcgct tggctgtggc agacggatat ccgggcggga
     2221 aattggccat gcaggcctgc gatctgcacc gccaatggct catgcagtcc tggccgcaat
     2281 attccaaaga agcacatatg ggtctggccc agatgtatgt ggatgatgaa cgatttgctg
     2341 cttactacga tcagattgct ccgggatgtg cagtcttcct gagagatgcg atgaaggttt
     2401 atactggaat ggagtaataa ctattccgct tgttttgggg agaaaacgca cagcaaaagc
     2461 cggggggctc ccccggcttt tgcttattcc cctctccgtt ggtagtcctc gtcgatgcgg
     2521 gctttccaat tggttttgat gctgcgggtt ttgcgcaagt cgctgacggc gtcattgacc
     2581 gcatcatagt tcagttgggt gcccgcgctg tcggcatggt agttggcggc ccggtcggcg
     2641 ttgatgccca gatcagctgc ggcggcgata gcatcgatgt tggctcccag gaagataaat
     2701 tcccagccgt attttttctg ctgttgttcc accaggctgc ggatctgttt ataacggtac
     2761 tcctggctgg cattttccat gccgtcggtg gtgatgacca acagcacttt ttcggcgcgt
     2821 tcctcctcgc tggtattctt ttgtacattg gcgatctttt gaatggtttt gccgatggca
     2881 tccagcaggg cggtgcagcc gcgcacatag tattcttttt cgctgatggg acgaatgcct
     2941 ttgaggttga tgcggtcgtg cagcagctcg tactggtcat cgaacagcac ggtggtgacc
     3001 acggcctcgc ccggttcctg ctgctgtttt ttcagcatgg cgttgtagcc gccgatagtg
     3061 tcgctttcca gtccggacat ggaaccgctg cggtcgagga tgaataccag ttcagtgaga
     3121 tcttttttca tcattgttat cctccttgtc tttgatgttt ttaggataac aaattcgttc
     3181 gctgtgttgg tcgccgagca ggcgacattt ttatgcaccc agcaatggca gttcgtagtg
     3241 gaagagcgcc tggttaatct caaatatatt atagttaccg gactgaataa agtattcaat
     3301 aacgacgtcg gatttgttgc tgcgggaaag cgcatagccg gcccgcccca gcaggtcgcg
     3361 ggtctcgtcc agactgagct gcaaggcaat ggcaaaggca agggcggtga gcttgctggg
     3421 ttgatagctg ggattgcttt tgatcttgga aaaaagttta cggtcaatat tggcccgttt
     3481 gtaggtctcc acgtcgtcct ggcctttctc atcgatgagg cgcagcagca tccgggaaaa
     3541 ggtctcgtcg agatgattga cgagttcatc cagcttgcgc ggagccaagt ccgcttgcat
     3601 ttggttgtaa tcgacctgaa cttcctccag caattcataa tgctggaggc gccgattcgc
     3661 gctgaacttt ttctgcagtt tctcaacata atggtcgtcg atgaattttt ccagggcggt
     3721 aaacagtttt tcgctcaaag cgagggattg ccggtcatac accaccaggt acaccatcat
     3781 ctcatgctgc aacaggaact cactgatggc ggctacggca atttgcagcg cctgatcctt
     3841 gggatagccg tagatgccgg aggaaatcag gggaaaagca atcgactcgc aatcatgctg
     3901 acgggccagt tccagggaat gaatgtagca gtcatgcagc agccgggctt cgtcggattg
     3961 tccgcccttc cagatgggcc cggcggtatg gatgatatat tgggccggca gttttcccgc
     4021 cccggtgatg accgccgagc cggtttggca tttaccgatg cggtcgcatt cggtttgcag
     4081 ctgctgcgct ccggcggcgg taaaaatcgc cccgcatacg cctccaccca tcttcagggc
     4141 ttgattggcg gcgttgacca ccgcgtctac ctccattttg gtaatatcct ggcgcacgat
     4201 ttgcaacggc atgcccgtca cctcctgctg tttttacggg tttcttatct atagtaattc
     4261 tgcgccgctg ctgtgattcc tgcctgcttt ccatgcgcac aaccctgtct gccggcagat
     4321 atcatggtat aatagattga tatacaaaat ttttaataac cgcaaaggac agcgtataaa
     4381 tgcgaattga aacagtctcc atcatcggcc tgggagcact gggaatcctg tttggcaacc
     4441 acctggccgg acaaatgccg gccgaaaacc tgcgcatcat agccgaccgg gagcgaatcg
     4501 cccgctacac ccgggaaggg atcttctgta acggccaacc ctgccatttt cactatgtca
     4561 ctccggagga gcaagggccg ccggcaaacc tgattatttt tgccgtgaaa tacgggggtt
     4621 tgaatgaagc catcagcgcg gtgcgccatc aagtaggaaa gcataccgtc ctcctgtcgc
     4681 tgctgaacgg aatcagcagc gaagagagta tcgcagcggc ttacgggacg gagcgggtgc
     4741 tcttctgtgt agctcagggc atggatgcgg tcaaggtggg caatcaaatg acctacgcca
     4801 atatggggat gatctgcttc ggcgaccggc agccagggat catttctgcc caagcacaag
     4861 cagtggcgga tttttttacc gcaatgcagc tgccacatca attggatacg gatatgaacc
     4921 gccgtatgtg gggcaaattg atgcttaatg tgggggtcaa ccaggccgtg gccctgttgg
     4981 gcagtgccta cggggatgtc caacgcgaag gcccggcccg ggaggtgatg gtcgctgcca
     5041 tgcgggaagt aatcgccttg tctcaaaagg aaaatgttaa tctgacggaa aatgacctgc
     5101 aatactggct gcaggtagtc agcaccctga gcccgctggg caaaccctcc atgcgccagg
     5161 acatcgaggc cggacgggcc agtgaactgg aactgtttgc cggcacgatc atccgcctgg
     5221 gagaaaaaca tcatctgccc acgcctgtca accaaatgct ctatgacggc atccgcagcc
     5281 tggaaaacgg ttttcaaccc agcgctcctg ataaagagag tccgtccggc agttccgcca
     5341 gcaccgacaa gtaacctgac tccttaatat agtttataac tatgtcgcgg catacgaatc
     5401 aagtattacc atatacctta cggccgtgct atatttttac cataagcagg ccgtcgtacg
     5461 gacacctgcc tgcaatatcc cgcctaaagg agtatgtggt tatgaagacc tcggatcatt
     5521 ttaaaaacaa gaccgttctg tcgctggagg tctttccgcc ccggcggact gcacccatcc
     5581 atctgattta cgagacgctg gaaggcctgc agggactaag cccggacttc atcagcgtga
     5641 cctacggagc cggcggcagc gaaatgaata ctgctaccct ggagatcgca tccgccatta
     5701 aaaaccatta cggcatcgag agcgtagccc atctgccttg tatcaacctc acccgcagcg
     5761 aggtgctgga tttgctggat ggtttcaggc aggccggcat tgaaaacatc ctggcactgc
     5821 gcggcgacat caacccggac ttcctgcccc aacaggattt tagatatgcc agcgagctgg
     5881 tatcctttat caaggaaaac ggagatttca atgtgatcgg ggcctgttat ccggaggggc
     5941 acagcgagag cgacagcttg gctgaggata tccgtcattt aaaaaacaag gtctatgcgg
     6001 gcaccgatca gctgatcacc caactgttct ttgacaatga tttcttctat tcctttcggg
     6061 aacgggcccg gcaggccggc atcaacgtgc cgatccaggc gggcatcatg ccggtggtga
     6121 acaagaagca gatcgagcgg atggttaccc tgtgcggagt gaagctgccc cgcaaattcg
     6181 taacgatgat gggcaaatac gagcataatc ccgtcgccat gcgggacgcg ggcattgcct
     6241 atgcagtcga ccagatcgtt gatctgatgg cggaaggggt cgacggaatt catctctata
     6301 ccatgaacaa tcccttgatt gcccagaaaa tttatgcggc catccgttcg ctgctggccg
     6361 cataacatca atcattataa aacagtgtgc ccgtaatcgg caataacccg cctcaactcg
     6421 gcgatatagt cagtggcaat ggtgctcaga tgcgcctttt cgctggcaat ccaccccacc
     6481 agcatgcgtt cctcggtctg cagcggcacg gacacaatgt tgtccccgtt gaggtcgctg
     6541 ttcagcacac cggtgctgat cgtatatccg ttcaggccga tcagcaagtt gaagagggtg
     6601 gcgcggtcgc tgacgtggat ggtcttgcga tggggcacgg tgctcaatat ttcctcggaa
     6661 aaatagaaag aattgtattc gccctgctca aaggccagaa tcggatagtc atccaggtct
     6721 tccagggtga cgcttttgcg cttcgccagc ggatgggagg aactaataaa gacatggggc
     6781 tgggcggcaa aaagcggatg aaaaagcaga tggttttcct tcagcagctt gttgagtact
     6841 ttttcattga agtcgctgaa atagaggatg ccgatttcgc tgcgcagatt ctttacatcc
     6901 tcgatgattt cccaggtcct ggtctcccgc agggtaaatt catactcatc cacgtccagt
     6961 ccccgcagca ggttgacgaa ggcattgacc gcaaaggcgt aatgctgggt cgaaaccgag
     7021 cagagctgct tggaaggctt tttgccgaga tagcgctgtt ccagtaattc catctgttcc
     7081 accacctgcc gggcgtaggc aaggaattcc gatccatcca ccgacagcga gatgcctttg
     7141 gaggtacggt aaaagatctc catgcccagt tccttttcca attcccggac tgcattcgaa
     7201 aggctcggct gggcaatata gagttgcttg gcggcttcac tgatggaacc gcactccacg
     7261 attttaatga tgtagcgtag ttgctgaagg gtcatgcttc acctgctcac cttttattcc
     7321 cgtcaataat cactggagga attgattgga ccggtcagtt ctccgtccct gccttcccgg
     7381 catccaggct ctccttcttg attatagcct aaatctttgc caataaaaat cctgcccgag
     7441 cgtttccgtc agggcaggcc ttgtaaagca gagccgattc acaagagatt cattttcaac
     7501 tcactgcccg gggcgggatg tacttcctgc cttttcctgc tgatgatgta agcgtttaca
     7561 caaatatcac ttagaagttg acggcggttg cggcggcctc gatttggttg gttcggcgcc
     7621 agatattcat tttttccgct tcttgcacca gcatgtctct aaaatctgga tgggcgatgt
     7681 tgatcagtgc ctccgccctt tcccaggtgc tcttgccctt catgtcggca atcccgtatt
     7741 cggtgaccag catatgggta gcggtgcggg gggaagtcac gatggctcct ggggtcagaa
     7801 tcggcctgat gcgcgagctg acctggcctt tgatctcaat cgtagacggc agacagatga
     7861 agctctggcc attttgggat ttataggccc cttccacgaa gtccaattgc ccgccggtgc
     7921 cgctgatgtg ccgggttccc accgtttctg agcagacctg cccgaacaga tcaacttcga
     7981 tgcaagcatt gatggacaca aaatcatcaa tttgggcaat gactttggga tcgttggtat
     8041 aatcgactga ataagaggcc aatccaggat tgtcgtccac ataatcatac agttcctgac
     8101 tgcccatgac gaaagagtag acctgcttgt agcggtcgat atttttacgt gatcccgtga
     8161 tcttgccggc tttaaacatt tccagataag catctacata catttcggta tgaacaccca
     8221 gattcttcaa atcagaggca gccaccattt ttccgacagc gttgggcgta ccgccgatcc
     8281 ctaattggat gcagttgccg ttatacaagc ggttaataat gtgtcgggcg atctcgactt
     8341 ccgcctggct gggctccgag gaacccaggg tgacgatggg ttggtttttg ctttcaataa
     8401 tataatccac ctggctgatg tgaacattct cctggttgcc gcccagacag cggggcattt
     8461 tgtcgttgac ctccaggatg gtgatgcggg cggtttccat cgccgcccag ccattgacgg
     8521 cgctgccgcc gaagttgaag tatccgtgtt tgtccatggt ggtaacctgc ataacaaaga
     8581 catcgtccgg cacacaatct ttgcgggtca tcatggggtt ttcatggaat ttcatgggga
     8641 tataaaataa atttttgccg ataaactttt tgtcctggcc tgacacgtgc caactgttcc
     8701 ataggaagtg tgccccttgc gggtcagcct cagcggtaaa gtgctggtag gcgccaatgt
     8761 cacagcggat ttttacgtcg gtcagctcat ccacccggcg ggccaatgct tcgtcaaaat
     8821 cccgggcaca gttgatgcca aacccatatt ccacccaatc gccggatttg accagtcggg
     8881 ctgcttcatc cgcggtgatc agttttttct tgtatttttc ctgccagttc atgattaagt
     8941 cctcctttat ccaatatcgt gaataagcga tcgtttcatt atttcgacta ccctaaaaca
     9001 atgcaatatc catgccagcc attaagggtc ttgttcaacg taataaagac cgattttatg
     9061 ccgaggtatc atttactccg ctgcaaaaat gaagcattgc gaaacaataa tgcaccagta
     9121 cataagaagt tatgccccgg tgcattaaat cacagcattc agtattattt cttgcgaaac
     9181 caggcgattt tattttccag tctcatctcc aattctccct ctgccgtcag gtgatgcaga
     9241 tgggacatgg cctctccgga ggcgaacagc ttctgtcccc aggggaagtc ctcccatttt
     9301 ttaaaggaca gatcccagcg catgcggctg gctacctggg tggtgttcat ggtttgatgc
     9361 ccgaggatat ccagaatatt atccaatctg ctgtgatgat gtcttttcag ctcttcgatg
     9421 cgtccctggc aatcagtgat cacgtaacgg tgtccaggca ggacagtatc gatttccagc
     9481 tgcccgatct tgtccaggct ctgcagatat tgacccaaca catcggtatc aaaattccag
     9541 gcggtgatgt tgggggtaat ttttcccagg atatgatctc cggaaaggag gagcttttta
     9601 ccctggtcat aaagacacat gtgtccgggt gtatgtccag gggtggctac acattgaaag
     9661 cggtaatcgc cagcggcgat ctcaaatcct tcctcaatca gattgaaatg ctgatatacc
     9721 tccgaggcga agcggtagcc gggatgtttg gtgaaatcat tttccacgcc gctgtcaacc
     9781 agtccgctga caattaaaaa accgttgaac atgctccagt gagagctatc ccggctggcc
     9841 tccaccacat tcccgtccgc ttcactcatc caaaccattg tatccggggc ggccagatgc
     9901 ttgaccagac cggaatggtc ggaatgcaga tgggtgataa acagatcggt gttttccatt
     9961 ttgacgccta gttcctgcag cccccggtcc atggcctgac gcgacacttc tgtgttaaag
    10021 ccggtgtcca ccagcagatt tcttttttct ccgcggatta tataggcgtt ggtcgctttc
    10081 agcggattgt tgggcagggg gatttcaatg cggtagatgc ccggcaatac ttgatcgatc
    10141 atatttttct cctttattta aaacatttac ctttttatct tttataaatg ttgcccaccc
    10201 gtttggggca atacatagat cacctgaatt taactaagat tactatggat tgactgcttt
    10261 cgtccaatgg tttatctgtt ataatggttt aaatttatat gtgattgcac aaaaaggttg
    10321 ggtattcgca tgcagcgatt gataaaaagt ttattcagcc gggttttttt gtttggtctg
    10381 gctattatta tccagctgtt tgtcctgata ctggtcatct ggagattcag caattacttt
    10441 gtgttttttt atgccatcct ggctgtggct tcgttgatcg ccgtgctggt tattttaaac
    10501 gggcgcagca atccaaccta taaactggcc tggatcgttc ctattctggt ctttcccatc
    10561 tttggcggct tgttttattt gatgtttggc gggagcaaat ccagccgcag aaacaaacgc
    10621 aaaatgctgg tcatcggttt aaaaatggag gactatctcc gccaggaacc ggatgttttg
    10681 cgggaattgg aacaggacaa ccccaaggct gccaaccaag ccagatacat tgacagttat
    10741 tctttttgcc cggtctacac gcacactacc gcaacatacc tgacgccggg cgaaattaag
    10801 tttcagcgac tgctggagga attgcgcaaa gccgaacgtt acatctttct tgaatacttc
    10861 attattgagg aaggaatcat gtggaatgcg gtgctggaga tcctggtgga aaaagcccgc
    10921 cagggagtgg atgtcagagt tatctacgat gatgtgggct gtctgatgaa actgccctac
    10981 gggtacgatc agaagctgga gcgtttaggc atcaaatgca gcatctttaa tcccttccgt
    11041 ccgttgttat cagtacgact caacaaccgg gaccatcgta aaatagcggt catcgacgga
    11101 cacacagctt ttaccggcgg gattaacctg gcagatgagt acattaatgc ctacgaacgg
    11161 ttcggccact ggaaagatgc ctccattgag atcaaaggcg atgctgtctg gaattttgtg
    11221 gtgatgttcc tgtccctgtg ggaatggctg cggggaggca atgaagacta cgagcggttt
    11281 cgcccctggc gcgagcatac ccgcaacttt gccagtgatg gtttcgtcca gcctttttcg
    11341 gatagtcctt ttgatgatga ggcgctgggg gaatcagttt acctgaacct gattaaccag
    11401 gcggaacgct atgtttatat caatacgcct tacctgatcc tggacaatga aatgcagacg
    11461 gcgctgcgac tggcagccaa acgcggagtg gacgtacgca tcgtgacgcc ccacatccca
    11521 gataaatggt atgtgcacgc ggtaacgcgc tccaactatg aaatactgat tgagaccgga
    11581 gtgcggattt atgaatatac gcctggattc atgcactcca aaaccgttgt ggtggatgat
    11641 cagttggcag tggtgagcac tatcaatctg gattaccgca gcttgttcct tcatttcgaa
    11701 tgcggcgtct ggctttacaa aaacaacacc attcaggaag tcagagagga ttttctgcag
    11761 accctgcggg tctgccagca tatcacttcg gaagactgcc gccatgtcaa atggtaccac
    11821 cgggtaattc gctcgttcct caggatgttt gccccgttga tgtagaaaat ttccatcaca
    11881 gccgtgcggg atcctttaat atttcagagc gatccatggc ccgggtatgt tccaccaggg
    11941 tttttaccag cagcccggtg ataatcccgg taggtaccga gaacaacagc ataaaaggca
    12001 gataatagat aatgccggca aattgcaaaa tcagggcagc catgaccagc tgcccgagat
    12061 tgtgagccgc tgctcccgcc acgctgactg cggcaatggc ggagccccgg ccaatataaa
    12121 ccagcagccc cattactata gtggacacga tcgccccgga aaaactcatc aaaaaaccca
    12181 ccccgaacaa agtcccgctc agcagcgagc ccaggatggt acgcagcacg gtgatttgca
    12241 tggcggtctt gaaatcgaaa agcagcaagg ccagcagggt gataatgttg gccaatccca
    12301 gtttgacccc gggaatggga atggggttgg gcagcaggct ttccagaaaa tgcagcactg
    12361 cggcttgtgc taccagcatg gcgatgatgg ccagtttttt agtgggcgtc aacctctgtc
    12421 cctcacttcc ggctaatata ttggtgacct gtactgattt attgccaaat cttcctcaaa
    12481 gctattatat aataaacagg acgggattgt cttattggaa ggagacgggt atgaccggga
    12541 ttaaactggg ccagtatgta cacggcgatt cgctgctgca ccgcctggac ccccgcacca
    12601 aaattatatg ctgcaccctc atcgtggtgg ccatcattat caatgatcgc tggtattccc
    12661 tgctcttttt cgccttgctg atggcggcgg cgattcgggc ctcgggtatg gacatccggt
    12721 ttgtgctggg cagtttgcgc agtatccgat cgctgttgct ggtcaccttt gtatttatgg
    12781 cggtgttgac tcccggccaa gcggtttgga cgtggggcgt gctgcacgtc acccgggaag
    12841 ggctaaccat gggggctgtc aatttactgc gcttgatcat tctttatctt ggttccatgg
    12901 tcttgctgat gacaacctca ccaatgcagc tgtcggcggg gattgattgg ctgctgctgc
    12961 cgctcagccg gctgaagctc ccggtccatc atttttccac cattctcagc atctcctttc
    13021 gttttatccc caccctgatc gatcaggccg ttttgattca ggatgcccag cgctcgcgcg
    13081 gggcacaatt tgcttctcct cgcttgggga tacgtttgaa gagttacctg gccattttga
    13141 ttcccctgtt tgaagcttcc cttcttcgag cggaggattt gggagaagcc atggacagcc
    13201 gctgctttac cggccatccc aaccaactgc gcctggaccg tctgcatttt cacgcagcgg
    13261 atgggtatgt gttgatttcc atgttgggat tgctggccgc cgggatcttg ttcccggttt
    13321 tgctgtgatg aaggaggctt cataatgctt gaagcgggta ttgttttaca aaatgtcagc
    13381 aaaatctacc gcagccataa cggtgacaga tgtaccctgg aggacatcaa cctgtccatc
    13441 cggccgggtg aatatgtggg actgatgggc atgaacggct cgggtaaatc gacgctggcc
    13501 aggctttgca acgggttgct gcttcccacc gccggcaagg tctatgtaga cggtctggat
    13561 accagccagg cggaaaatct gcctgccatc cgccagcggg tagggatggt ttttcagaat
    13621 cctgacaatc agctgatctg cccggtggta gaggaagaga tcgccttcgg gctggagaat
    13681 ttgggcctgt ttctgccgga aatcagggaa cgcactgcct gggcccttga gatcatggga
    13741 ctaaccgggc tagagtatca tgctccccat ttattgtcag gaggacaaaa acaaaaggtg
    13801 gccctggctt cggtactggc catgaaaccc gcctacctgg tgctggatga accggccgcc
    13861 atgctggatc cggtgagccg gcgggaactg ttgcagcatc tgaagatcct taatcgtcgg
    13921 gaaggcatga ccattgtgct gtgcagcaat gaagccgagg attttatgga tgctgaccga
    13981 ttgctggtaa ttgaccgggg caccctatgc ctacagggaa cgcccccgga agttttgtct
    14041 caaggagaac aactggcttc catcggcctg gcccagccgg atttttaccg actccggcaa
    14101 cagctggcag acgcgggctg ttcggtaaca gcaatctata atcttacgga tttggtggat
    14161 tatctatgtc atccataatt gaagttaaac aactgcatta tacctatttg cctggtacac
    14221 cttacgaacg ccaggccctt aaaaacattt ccatcgcggt ggaagcgggt gattttgtag
    14281 gcatttttgg ccccaacgga tcaggcaaat caaccttggc ccgacagttc aacggactgc
    14341 tccgtcccac gcaggggcag gtcctggttt gtggcaaaga tacgaccaat gagaagattc
    14401 gacgatccct atggaataaa gtcggtctgg tcttccagtt tcctgaacaa cagatttttc
    14461 agaccactgt ttataatgag attgcctatg gtccgcgtaa cctgggatta ccagaagagg
    14521 aaatcagaga gcgggttggg caggctttgg ccatggttgg attggcaacg caagatatcg
    14581 ccaatttgtc accttttagt ctcagcggag gcttgaaaag aagggtcgcc atcgccggcc
    14641 tgctggcctt gcacccggag attctaatcc tggacgaacc aatggcgggt cttgatccgg
    14701 ttggccgccg aatgataatg gagatcatcc ggcagcgccg ggaaaataat gccacaacgc
    14761 tgatgatttc ccatgacttg caagaaattc tcaatgtggc tgataaaatt gtcattctgg
    14821 atgagggggt cctggttttc ttcggtaaga ccagcgaatt gctggatcat cctgacattt
    14881 tgcagtccta tcgcctgcaa ctgcctttat atttacaggt ggtatatgcc ttgcaggaaa
    14941 aaaaacaacc ggtcaaacaa aacattggct ccctcgagga agccgccgcg gaaatcataa
    15001 aactgttccc ttagtcctcg atcctgacta ccgcccggtt gggcatgcag actgccacgt
    15061 cccccgggtc ttccagccag ccggtattga cacaaatctt gtcgggacag tcggcttgtt
    15121 taaaccgaat gcggccgggt tctacttcga cgacttcatg atatttgccg ggcagagtaa
    15181 tctggcgagg ctctcttacc cgctgtaaat caatacgttc gaccaatcgt ccgtcctggg
    15241 tgatcacggc aaccctgctg tcgcctgaat tggcctgttg taaataccgg attgcccctg
    15301 ccaccaacac gatgaggatc gtaagcgaca cgagcagaac ccattctttt ttgcgcatat
    15361 cagcaaaact cctgtttatc ttctcccgcg ccgtgatgcc cggtggtttt acaaccatcg
    15421 caggtatgtg tggtcagcga ttcttgtgac ggcgctgcat aagttcttcc gtttcccgat
    15481 gaacttcctg taaataaaaa tctctatcga ggtccaacgc tgcaaaaaaa tatttttgca
    15541 gaccgtctgc cagctcaatt ttccttgcgg taaaggccgg ttcatagtga agaagaaaag
    15601 tcaacaatct ggccacatca ctttctatcc ggccaggacg acactcttca aaatcgaccc
    15661 cgtagacttg tccggatgtt tcatgataga tgaaattgcg caggtgaata tcccccagga
    15721 tatactgctg cccggttttt tcacgcatgg tttggtaaaa tttcatcatc cagtcacaaa
    15781 cctggatcat cattgcctga acttgttccc attctgctgt ttcaataaga tcgaccagcg
    15841 ccatggcagg aatatactca taaatgatct gctgttgccg agtattgata tcaatacgtc
    15901 tcggggtgag caagccactg tccttcaaca gggtaccgat ctctttttcc atacagaaac
    15961 gctgcgaatc ggtaaattgt ttgaccacca cttcctgctg tccgttcctt tcccggtata
    16021 tatggttctt tttgctgggc agcggctcac gcttgggcat gatgagcatc ctctccaatc
    16081 cagtcgggtt gttcctccca gaaggaaacg gtctgttcga tcaggtcagt caatctgaca
    16141 acttcggtca gcgcgttgat caccggcagt ccctggtatt cctgcagccg cccggcaatc
    16201 cgcccggtca ccgcgattac cttgtcccat ctgcgttcat caatctcatt ttcctccacc
    16261 gccgcgatca gtcccggtcc tttaaaatca taggcccctt ccagaatgac aaagtcctga
    16321 tcataaaagc gcaaaacttc cgtgatggtc aagcgtgata gaaacattac atcggtttca
    16381 taaagacccc gggcggtcac caactctgcc ccggccattt tgtgtcgatg ggtgttggtc
    16441 ccttcctggt ccagggcaaa accttcaaag tgaatgtcct tgaccgatcc cacacgatag
    16501 tttctgcgcc gcagctccgc aatgattgct tcggccacgg tggttttgcc tgaccgggaa
    16561 atccctacga tggaaaagac tttcacgtta tccccagcct cctttcggtt catcaatcaa
    16621 aaacttgacc aaaggtatgt gtcatcgtat gaaacaaaca tagtatccgc cgattagaat
    16681 tacagtgact acgatcgcac tgagcatgat tacatagtcc cgtagtttta actccaggac
    16741 cagataactg gtacgggtgg gataggctcg aaaaccacgc atctcgatgg acatggcaat
    16801 atccttggat tttaatattg cacctgctac caccggctga aacaaactgg cggtaacttc
    16861 cagcttttga cgcaggggta gttttttgat atctacccct cttaactgca cggctatcat
    16921 ggcatcccga aactcttctg caaaaacagg cagaaaacgg atgcccatgg aggccataaa
    16981 agcaatctca tagggcagtt tccattgcac cagaccctgt atgatctccc ggtgattgga
    17041 tgttgtcaaa atgcctgccg aggtaatgat gatcaacatt cgcaagagaa actcggcacc
    17101 cttgagaagt cctccggcag ttaccaattt tacgctgcca acagccagca gggtctggcc
    17161 agccggggca aacacgcttt gcagcaaggc gataacaatc atcatgtaca gcaacagcct
    17221 aatccttttt atgatactcc aaagacttac accaaagcag gacagcagcc caaaggacac
    17281 cagacacaga ccactcagga aaatccaatt gctgataagc accgctgctg aagtcaggca
    17341 catgatgaga atcagtttgg tgcgcggatc caagtcagac acggaggtca tcgatgatcg
    17401 aacctccttt catgggcagg atgcgcccgg cattatcgcg gacgaaatcc tggtcatggc
    17461 tgattagcag catgccgatg ccgttgtccc gtaatctggc cagcacccgg ttgaaagtcc
    17521 ggattcgttc tgcgtccagt ccgacggtag gctcatccag caccagatat tgcggttcga
    17581 gggccaaaca ggcggccagc accacacgcc ttttctcgcc ccagctcaaa tttaggggaa
    17641 aaaagtggcg caggtgctcg atttcgaaca gttccagcag gtaattaaca gtggcgtcga
    17701 tcaattcccg gcgaacgccc cgataaacaa gcccaaaggc gatttcttcc tcaacggtgg
    17761 cagccagtaa ttgctgacga gggttttgaa agcagtatcc caccttttcc ccaatcaggg
    17821 acagcggcag ggaacgggca tcttccccaa ataacaacaa ctgtccggca gcaggcttga
    17881 ggattccagt caacagtttg cccaaggtag tcttgcctgc tccattgggc ccgatcaagg
    17941 caactgtttc tcctttgtat aaacaaagat tgagctggtc gatcaaagca gcctcccgag
    18001 gatagccaaa cgacaattgc cgggcttcaa tggcgggttc tctatttatg gctgctcacc
    18061 tcctgtccag gcgaactgtt gcaagcggcc ctctgccagc gaccagatct ggtcggcgat
    18121 atcgatgatt ctgctgtcat gctcgatcat aataatggtg cagccttgcc ttttgagagc
    18181 cagcagcact cttttaaccc ttgcgcgaac ggtttgatcc aattgagaaa gcgtctcgtc
    18241 taaaatgatg atgtccggcg ccagagtcag tacgccgccg agagcgacca gttgtttctg
    18301 tccacccgac agacgggctg gatttttcag gcgcagctcc tctatattca acagttccag
    18361 aatgccattg atgcggtctt caatctcctg tcggggcaga caaaggttct ccgggccaaa
    18421 ggccagctca tcttctaccg tgggcagaaa tacttgggca tcggcatcct gaaaaacgat
    18481 tccgatcctg ccctgacggg tgatccgccc actgaattcg cctttgatga gcgtggggat
    18541 aacaccgctc aggcaatacc ccagagtact tttaccgcct cctgaaggac cggtaagtgc
    18601 agttatttga cctgcttcca aagacaaggt aacattttcc agaatcattt ggggttgtcc
    18661 cgaatagcga aaggaaatcg attttgcttc caacatgcgc atgactgttt ttccgccttt
    18721 tttattgcac atccaattca catacatatt tcgcccagcg ctggctgaag cggtcacccc
    18781 gaatgacaat catatagggg ccttgtccgt cggcttcctc ataagatcca agataattgc
    18841 cgttatcctt gtaaaccaga tagatgttgt caaattcttt tatttcttta attgaaagcg
    18901 gcaccacata accatccacc gaataaacca gtacccgttg cttttcttcc agcgatatcc
    18961 ccgcagcggt aaacagatca gcaaaagcga tgccggtata ggtatgctct ttcggttttt
    19021 ccattaggct gctctttagc cgggcagtaa actccacggt tttcatcgaa cccagaacgg
    19081 tcatgttgag gcgtgctact tcctgaccgc tttcccgaac ggcaatttcc gttgcctcca
    19141 gcaaagcctt cctgtccgct gcataacgct ggtttacgat cacgccgatg gtcgtggtga
    19201 taaacagaac tgcaagtata ataacaataa tccggttgtt tttgttcatt atgtccattc
    19261 ctattaagag atgttggcaa tgctaatcaa atattaagtt tgctgaaacg ttccgccacc
    19321 agccacgcca gcaagccgcc caaggcacct gacagcgccc cggcactcag gctgaagagc
    19381 agcggaatca gcggcaaacg gaaaaacacc aggttggaca gcagcacgcc gcaaagattg
    19441 gcgccgatgc agcctgcaaa catggccatt ttctcatctg ccttgagccg ggcgatccag
    19501 taaagcagat ccaccgtcag tcccggcagg gtataggtga ccagactgac gatgccgtgc
    19561 gtgccgtaaa agccggttac gatgaccatg atggactgca ccagtccgat cagggcagcg
    19621 gtacccggtt tttttaccag ggccgcaccc agtaccagcc acagcatata aaagccgccc
    19681 gccagcgatc cgccgggcat aatcagcggg ccggtaataa tatggaccag tcctgtcact
    19741 atcggtttga ccgccacccc cagcgctgcc atcaaggcga tgatgaccag gtcaaaagct
    19801 ccgaatctct tcaacaaatt gttcatttat caatgattac tccagcttta atgttgtttc
    19861 actgcgctct ttgatct
//
LOCUS       DAOTHZ010000038        19187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3432_length_19187_cov_7.349467, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000038 DAOTHZ010000000
VERSION     DAOTHZ010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_19187_cov_7.349467"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /gene="dapF"
                     /locus_tag="P5549_05990"
     CDS             complement(<1..422)
                     /gene="dapF"
                     /locus_tag="P5549_05990"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRW12658.1"
                     /translation="MEFVKMHGLGNDFIIVEAASWKDADPLQKYSPWLCNRNFGIGAD
                     GLVVIGPEQEMDIFMRIFNPDGSEPEMCGNAIRCVARYAYEKGWVRKKNISVKTLAGG
                     RYPEIQTAADQAVRVLVDMGQPILDRPNIPMLGEGPAV"
     gene            complement(475..3186)
                     /locus_tag="P5549_05995"
     CDS             complement(475..3186)
                     /locus_tag="P5549_05995"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRW12659.1"
                     /translation="MTKYAWWNVTFAEIAKLLKTDAENGLAEPEALRRQELFPNRIVS
                     SPPLHPAFLLLDQFTDTMVLVLLVATVISGLIGAMGDAITIMAIVVLNAVLGFIQEYR
                     AEKSLEEIKKLASPTAVVIRSGLRKRIAAAEVVPGDLIIIEAGDKVPADLRLVYSSNL
                     EVDESALTGESLPVSKKADQLLARETGLADQINMCFMGTAVTRGRGRALVIATGMDTV
                     MGEIAAMMKEPGKVLTPLQNRLDQLGKVLIIVCIAVCALVAGLGILRGEGWLTMLMAG
                     ISLAVAAIPEGLPAIVTVVLGLGVQRMAKQNAIIRKLPAVETLGCTTVICSDKTGTLT
                     RNQMTVKKIAAYDLEMEIAGEGYQKEGKFTYQGQTINPLHIRSVQMIITAALQCNQAV
                     LQKGKTGCEVEGDPTEGALLILAAKAGLQQRLPILREIPFDANRKRMTVVVQQQGEYL
                     VLVKGALDVLLPFCEQVMQADGVHRLEQKERSYFLQRQTQWAEDAMRVLGFAYKTLPA
                     GSWEKMSDEQLESGLILSGICGMIDPPRKGAARSIKQCLTAGIIPVMITGDHPLTASA
                     IARSIGLVSGREVLRGPEIDQLDDEQLYRRVIRDRVVARVTPQHKNRIVQVLQFHDQV
                     VAMTGDGINDAPAIKSADIGIAMGISGTEASKEASSMVLTDDDFSTIVKAVHEGRAIY
                     DNIRKFIRYLLGCNIGEVLVMLLASLLALPLPLLPIQILWVNLVTDGLPAMALGVEPP
                     EPGIMKRKPRSKNESIFAHHLALTICSRGIYIAVITIATFLLAWGWSAAQGTEGLHLA
                     RTMAFTTLVFAQLFYVFECRSEKLSPFELGFGKNKFLLAALCFSILMQLSVIYLPFFQ
                     DIFKTVPLQPWHWLLILSLSGMKFLGRLFVYTWQRVFVSPGRYVKMG"
     gene            complement(3353..3991)
                     /locus_tag="P5549_06000"
     CDS             complement(3353..3991)
                     /locus_tag="P5549_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12660.1"
                     /translation="MMKKGTREQNIKKIINMEDRIIKCDRCQSLTRCIRRPSLGKGDL
                     EPELLMVFEYDNPFTMKREAVLNLRDTIKNHLGTEKIYYIFLARCQPKACAVRENANC
                     YMSNKLIDKELNCLLTNAKCEGIPIKPSVDVLMNCLPYLMEEIEILQPEYVILFGNRV
                     GDYVLKSCGYFERAENNHAFNYNGSHFMISVEETNFTEEHALELKALMQAQA"
     gene            complement(4025..4405)
                     /locus_tag="P5549_06005"
     CDS             complement(4025..4405)
                     /locus_tag="P5549_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12661.1"
                     /translation="MEQELFGIPSTILGKEDWKELVRREDTIGPEQLLNEILSKRLWS
                     NAEIVWVIKRMVYFYGKKDHLLKKAPADRLFMNMVDILRCFFLVMDKEDPALDDNIRA
                     YMSSKIADSTWGINQRTREYLEKF"
     gene            complement(4441..5286)
                     /locus_tag="P5549_06010"
     CDS             complement(4441..5286)
                     /locus_tag="P5549_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRW12662.1"
                     /translation="MKKKTGIFIATLLLWTCMSAIALAVPANYTVQAGDSLWKISNQF
                     GVSIDQIKKLNGLQSDSLQIGQKLILSSIANQTPAPAPAPAPAVNAPAASGTDVYVVK
                     SGDTLWAIARQFGTSIANIKEINGLTTDALKIGQTLKINASAAVVAVPVSRSGDSASA
                     ARLLEDAAQYLGTPYKYGGSGPGGFDCSGFTSFLYARAGYSLPRTAAGQYSVGAAVAQ
                     SELQPGDLLFFAGGGKGIDHVGIYAGNGQMIHSSSPRSGGVIYTPLSSSYYAKSYVGA
                     RRVLR"
     gene            complement(5385..6227)
                     /locus_tag="P5549_06015"
     CDS             complement(5385..6227)
                     /locus_tag="P5549_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRW12663.1"
                     /translation="MMRCEYDLHVHSTYSDGTLSPPALLAQAMEIGLTGISITDHDTL
                     DGLQETLAYIADHTIKIDYIPGVELNTEPEEEGNEIHILGYYIAHENRGFHQRLAEIR
                     EQRKNRAIKIVEKLNALGCKLDFANVSRQAGSDLIGRPHIAMAMVEAGYAKSMAEVFE
                     QYIGYGRPAYVRRYKFPPGEAIELIKSSGGIAVLAHPGLIKNQALIGNLIKAGIEGVE
                     TYYPQHTEQQTTAYLKLAEEHKLLITGGSDYHGPGSSESRAHLGGSGIDSVLMAKLKA
                     YKYK"
     gene            complement(6235..7161)
                     /locus_tag="P5549_06020"
     CDS             complement(6235..7161)
                     /locus_tag="P5549_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRW12664.1"
                     /translation="MPIADLHCDTISFLTQNQLRLACNHGQYDLQRAAQAGIGLQFFA
                     MFTMPDEPDRALRAISKQLNRFYQEVEANEEKIKLVTSFQDIKTADQAGKISAILHLE
                     GAECLGSDLDTLHLLYRWGLRSIGLTWNARNQFACGVAEGDAGGGLSKLGRMLAEEMD
                     QLGMVMDLAHMAKASFYDVLEYYNKPVLATHANSFRLCPHPRNLDDDQLKKLAEHRGL
                     IGITQVADFVGMERQDEAAMLDHLVYIADLIGVDHVALGSDFDGADKMIIKDVSGYQQ
                     LPQLMKKRGFSQEETEKILYRNVVNTLASILM"
     gene            complement(7329..7589)
                     /locus_tag="P5549_06025"
     CDS             complement(7329..7589)
                     /locus_tag="P5549_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRW12665.1"
                     /translation="MEVLKVSAKSSPNSVAGALAGVLREKGSAEIQAIGAGAINQAIK
                     AIAIARGFVAPSGMDLICVPAFTDIMIDGEERTAIKLIIEPR"
     gene            complement(7708..8505)
                     /locus_tag="P5549_06030"
     CDS             complement(7708..8505)
                     /locus_tag="P5549_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRW12666.1"
                     /translation="MNILVIGDIVGKPGREILKSLLAWVQKKYQIDFTIANGENAAGG
                     RGLTLEVKNELLGMGIDCLTMGNHVWDNKNIFTFIDDEPRLIRPINYPGDCPGQGMHI
                     YNAGFNKKIAVINASGRVFLPALDCPFRALDEALQEVEASADYILVDFHAEATSEKVA
                     FGYYLDGRVSAVVGTHTHIQTADERILPQGTAYITDLGMTGPIDSILGMEKQPIIERF
                     LNARPSRFQVAEGPAHLQGVVIELAEDRRQAVRIERISHILSNKGIT"
     gene            complement(8521..9012)
                     /locus_tag="P5549_06035"
     CDS             complement(8521..9012)
                     /locus_tag="P5549_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12667.1"
                     /translation="MDIEAALDAVISVFIRNPEISSIQYQAEQNVIAIEMIITENIDV
                     QDNLMICRNLEAALGMIHKLRGTEPKKLGIELNNQGNIGILVLRRDALTLSEEEVDLY
                     VQLAGQKFEAMLLRENDRNSLLKNRFDDIKKQLLQSLNQPDRLTRNIFAYRDRGKMFV
                     FDR"
     gene            complement(9267..10817)
                     /gene="rny"
                     /locus_tag="P5549_06040"
     CDS             complement(9267..10817)
                     /gene="rny"
                     /locus_tag="P5549_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRW12668.1"
                     /translation="MKKINPLVVLVISIIALAIGIAGGYYLRKIVAESKMGSAEEEAA
                     RTIDEATKEGEAKKKELLLEAKDEIHRNRQEFEKDLRERRRELDRLERRVLQKEELVE
                     KKSENIEKKEQNLVEKEKDIEKTQQDLQLILTQQLKELERLSGLTSEEAKELLLHNVE
                     QQIRQETAVMIRTLEAEAKEESDKRAKNIVSLAIQRCAADVVSETTVSVVALPNDEMK
                     GRIIGREGRNIRTFETLTGVDLIIDDTPEAVILSAFDPVRRETARLALEHLVSDGRIH
                     PARIEEMVEKAKKEVDQELKEVGEQAAFEVGVHGLHPELIKLLGRLKYRTSYGQNVLK
                     HSIEVAHLAGVMAAELDVDVALAKRAGLLHDIGKAVDHEVAGPHVEIGVDLAKKYRES
                     KDVIHGIEAHHGDIEPHTIEAVLVQAADAISASRPGARRETLENYIKRLEKLENLADS
                     FAGVDKSFAIQAGREIRIIVKPDEIDDLETITLVKDIARKVEQELDYPGQIKVMVIRE
                     TRQVEFAK"
     gene            complement(11169..12182)
                     /gene="recA"
                     /locus_tag="P5549_06045"
     CDS             complement(11169..12182)
                     /gene="recA"
                     /locus_tag="P5549_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRW12669.1"
                     /translation="MQGRTEALNNAIRNIEKIYGKGSIMKLGESTTMNIESISTGCMS
                     LDLATGVGGLPRGRVIEIFGPESSGKTTVTLHVIAQAQKDGGIAAFIDAEHALDPMYA
                     KQLGVDINNLLVSQPDSGEQALEIAEALVRSNAIDVIVIDSVAALVPKAELDGEMGDV
                     HVGLQARLMSQALRKLTAHIGKSTCCVIFINQIREKVGVIYGNPEVTTGGRALKFYSS
                     MRLEIRKGEPIKMGNDITGNRTKVKVVKNKVAPPFKSVEFDIMYGTGISREGDLLDVA
                     SELNIVQKSGSWYSYDGERMGQGRENAKEYLKNNPEIFLTLQKLIREKMFPTVAKIET
                     EEA"
     gene            complement(12271..12831)
                     /gene="thpR"
                     /locus_tag="P5549_06050"
     CDS             complement(12271..12831)
                     /gene="thpR"
                     /locus_tag="P5549_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRW12670.1"
                     /translation="MRVFAAIPLPDDIKQYARMMRNKLGIARPDIKWVEYQNYHLTVK
                     FLGEVDFNDLPEIKRNLRLAADSSPAHNLSAGGIGFFPNQAQPRVMWMGIKGEINKTE
                     FLCDRVDAYLSPLGFAPEKDHRLHLTLGRIRSDKNLKEMLNLLEKYPDRNKLRSFRVE
                     QFYLMQSVLDGNGPQYHVLDTYTLNG"
     gene            complement(12846..14087)
                     /locus_tag="P5549_06055"
     CDS             complement(12846..14087)
                     /locus_tag="P5549_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRW12671.1"
                     /translation="MPKVFIISTGTELLLGTTHDSNSEYLAQQLEDIGLRVAGKVTVG
                     DSREQIQMAFAAGLETADIIVSTGGLGPTFDDLTKTVACELMGCSLVVRPEEEQRLRD
                     FFAQRRRPMPEINMKQAMFPVEAEVLSNTQGTAPGMYLRRQNKLLILLPGPPREMTRM
                     YLSEVKPRLERDYADQLQKVLRRTIKVIGPGESQVEEMLADMLNNSSGYSAALLAQEG
                     EIHIKLAIAGGQSTDSIPRLNELTKLVQHKMHKHIFGYDDETLPEQTGKLLAARGRKL
                     AVAESCTAGMLGSMITEVSGSSDYFWGGVITYSNDSKMKLLNVQASTLEQYGAVSQQT
                     AEEMARGIRQLSGVDYGLAITGIAGPCGGTREKPVGLVYVALAGAQTCRVRELKLGGS
                     REIVRRLSAKSALDLLRRHID"
     gene            complement(14236..15705)
                     /locus_tag="P5549_06060"
     CDS             complement(14236..15705)
                     /locus_tag="P5549_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW12672.1"
                     /translation="MKSILIGAGLGIMIFLWLNGFNMTPVIVAAALIFAVLYLLPGQG
                     RMKLSNSDERRDKQSVLEFTEIGGQDSAINELQEALQFVMQPEKIDHMGIRPLKGILL
                     AGPPGTGKTLLARAAASFTSSAFVAASGSEFIEMYAGVGAKRIRQIFASARQEARKLN
                     KKSAIIFIDELEVLAAKRGSHSGHMEYDQTLNQLLVEMDGLTQNRDPRILLIGATNRP
                     DLLDPAVLRPGRFDRQVQVDLPDRCGRGKILAIHTRNKPLDPTVDLDDIARATFGFSG
                     AHLESLTNEAAILAMRENSTLIQQTHFREAIDKVMMGEKLDRQPSSSEMERISIHESG
                     HALVCEYLEEGSVASLTIIPRGKALGYMRRSPQDDQYLYTYEELEQQIMIMLAGALAE
                     EIAFASRSSGARNDFQQAWQVAREMISSGLSSLGVVDDDSLPAEVVYKEGKRIITAAE
                     NKTRDLLQDNRERLILLASGLRQQESLGRASLEKILYAS"
     gene            complement(15705..16253)
                     /locus_tag="P5549_06065"
     CDS             complement(15705..16253)
                     /locus_tag="P5549_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12673.1"
                     /translation="MKNHEIKVALVLLGLLLTLGLLVGGQKIYRADMVEKPVVKQLLA
                     LPCVDEAVLCNDQGRNTINVHMQSVDNLKQAYNEIDQIMAAKFPRGNYGIVLDDQRNA
                     ALQNEWEQLELALYEAIASNQYVALEQHLNAVAARNGFTCQLQIDTQHLFVHLQRGEQ
                     FLYEIIERQPPDSIEAANGEKS"
     gene            complement(16404..16940)
                     /gene="pgsA"
                     /locus_tag="P5549_06070"
     CDS             complement(16404..16940)
                     /gene="pgsA"
                     /locus_tag="P5549_06070"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRW12674.1"
                     /translation="MNLPNSLTLLRIFLVPVFVVVLVLHIPYGDLLAAIIFVAAALTD
                     SLDGYLARKYKQVTKLGIILDPIADKLLITAALICLVELGRLQGWIAIVILGREFAVS
                     GLRAVRAEEGIIIPASKLGKFKTISQIVAVIIIILESLYPPLHIIGVWAIYIALAITV
                     ISGLDYFMKFRSSEDQNQ"
     gene            complement(16924..18282)
                     /gene="rimO"
                     /locus_tag="P5549_06075"
     CDS             complement(16924..18282)
                     /gene="rimO"
                     /locus_tag="P5549_06075"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRW12675.1"
                     /translation="MTTIKNHQYKIGFISLGCAKNQVDSEIMIARLKSAGYKIVSNME
                     RADIVILNSCGFIEDARQESINAIIEAGQLKERGMLQYLVCTGCLAQRYGQELLDELP
                     ELDGLIGISTFPAIVQHLERILQGERLLAIEPPPPLFIEKGPRVLTTPPGSAYLKITE
                     GCNNRCSYCAIPLIRGNLRSRPAGELLAEARDLAGKGIKELVLIGQDTAVYGKDLSGT
                     CDLGGLLQQLSKIEELPWIRLLYLHPAHIDQRIIDTVAGGDKVLPYLDIPIQHAADPM
                     LKAMNRKHNAADLKNLFSRLRAQVDGLVLRTTVMLGFPGETEDDFKILYDFLGETEFD
                     WLGAFAFTPEAGTPAANLAGQVPEEIKQARLASIMKLQKKITRKKNKKRLQQNYEILI
                     SSQLSPHLYLGRGYYQAPAVDGITMVKSERRLTKGEFIKVELKAIRNYDLIGEIVDEF
                     TK"
     gene            complement(18269..18997)
                     /locus_tag="P5549_06080"
     CDS             complement(18269..18997)
                     /locus_tag="P5549_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRW12676.1"
                     /translation="MDGFGERLRATREWLGITLDEVEAETKIRKLYIEALENENFAIL
                     PPQVYAIGFVKQYARVLKLDEELVCAEFKQVAYPEVIPEPVAEKPVRKRRKIQLPRLP
                     YRNLAGAVVFLLLAIWVGNLFANYLGDKMTPAPPAPPVQSGEKNPPAADQSEAPAAVP
                     MEIRVKVKPGMQCWIRVVTDGGNKTEMTLAEEQEETFTARANLQLRLGNAGAVDVTVN
                     GKPVAALGEMGQVVEKEFKNSDNY"
BASE COUNT         4626 a         5039 c         4207 g         5315 t
ORIGIN      
        1 ttgactgccg gtccttctcc cagcatggga atgtttggcc gatccagtat gggttgtccc
       61 atgtcaacga gcactctcac cgcttggtcg gcagcggtct gaatttcagg atagcggcct
      121 cctgccaagg ttttgaccga aatgtttttt ttcctgaccc agcctttctc ataagcatag
      181 cgggctacac agcgaatggc attgccgcac atctcaggtt cactgccatc tgggttgaag
      241 attctcataa atatatccat ctcttgctcc gggccgatga cgactaggcc atcggcgcca
      301 atgccaaaat tgcgattgca cagccaaggt gaatactttt gcagcgggtc ggcgtctttc
      361 caggatgctg cttcaacaat aatgaaatcg ttgcccagac catgcatttt tacaaattcc
      421 atcgttctcc tcctattacg ccggattagc gctcatttac cagtttttga acagctatcc
      481 cattttaaca tatctcccgg gggaaacaaa aaccctttgc caggtataca caaacaatcg
      541 tcccagaaac ttcatccctg acagggacag aatgagcagc cagtgccagg gctgcagcgg
      601 cacggtttta aaaatgtctt gaaaaaatgg taaataaatc acgctcagct gcattagaat
      661 tgaaaaacat aatgcggcaa gcaagaattt gtttttaccg aaaccgagct caaaaggaga
      721 cagtttttcc gaacggcatt caaaaacata aaacaactgg gcaaatacca aggtggtaaa
      781 agccatggtc cgggccagat gaagtccctc cgttccctgc gctgcgctcc agccccaggc
      841 aagcaaaaaa gtggcaattg tgatcacggc aatatagatt ccccgactgc aaatcgtcag
      901 cgccaaatgg tgggcaaaaa tactctcgtt tttggaacgg ggcttgcgtt tcatgatgcc
      961 gggttcaggt ggttcaaccc ccaaagccat ggcaggcaga ccgtcggtaa ccagatttac
     1021 ccacagaatc tgaatcggca gcagcggcag cggcagcgcc agcaatgatg ccagcaacat
     1081 gaccaatact tcgccgatgt tgcaacccag cagataacga ataaactttc tgatattatc
     1141 ataaatggcc cggccttcat gaacagcttt cacaatggta gaaaagtcat catctgtcag
     1201 taccatggag gaggcttcct tgcttgcttc ggtaccgctg atccccatag caataccaat
     1261 gtcggcagat tttatagcag gcgcatcatt gatgccgtca ccggtcatgg caactacctg
     1321 atcatggaac tgaagcacct ggacgatgcg gtttttgtgc tggggcgtga ctctggccac
     1381 cacccggtct cgaatgaccc gtctatacaa ttgctcatca tcgagttgat cgatttcagg
     1441 cccgcgcagc acctcccgac cagagaccag accgatactg cgagcgatgg cgctggcggt
     1501 taaaggatga tcgccggtaa tcatcaccgg aataatgccg gctgtcaggc attgtttgat
     1561 ggagcgcgcc gctcctttac ggggagggtc aatcataccg cagatcccgg atagaatcaa
     1621 gcctgattcc aattgctcgt cactcatttt ttcccatgac ccggctggca aagtcttgta
     1681 agcaaaacct agcacgcgca tggcatcttc cgcccattgg gtttgccgtt gcaggaaata
     1741 actgcgctct ttttgttcca agcgatgaac tccatccgcc tgcattacct gctcacaaaa
     1801 aggaagcagg acatccagcg cacctttgac cagaaccaga tattcaccct gctgttgcac
     1861 aactacggtc atgcgtttgc ggttggcatc aaacggaatc tcccgcaaga ttggcagacg
     1921 ttgctgcaga ccggctttgg ctgctaaaat cagcaatgca ccttcggtgg gatcgccttc
     1981 tacttcgcag ccggttttcc ccttttgcag cacagcctga ttgcattgca gggctgcagt
     2041 aatgatcatt tgaacgctcc tgatatgcag cggatttatc gtttgaccct gataagtaaa
     2101 tttcccttct ttttgataac cttctcccgc tatctccatt tccaggtcgt aggcagctat
     2161 tttttttacc gtcatctgat tacgggtaag cgttccggtt ttgtcagagc aaatcactgt
     2221 cgtacagcca agcgtttcaa ctgccggcaa tttcctgatg atggcatttt gtttggccat
     2281 cctctggacg cccagtccca gcactaccgt aacaattgcc ggcagtcctt ccggaatggc
     2341 ggcaacggcc agactgattc ctgccatgag catggtgagc cacccttccc cccgcaggat
     2401 tcctagtcct gccaccaggg cacaaacagc aatgcaaacg ataattaaga ctttacccaa
     2461 ttgatccaat ctgttctgca ggggggtcag gactttgccc ggctctttca tcatggccgc
     2521 aatctctccc atcacggtgt ccatgccggt ggcgattacc agagcgcggc cgcggccgcg
     2581 ggtaacagct gttcccataa aacacatatt gatttgatca gccagtccgg tctcccgggc
     2641 aagaagttgg tccgcttttt tgctgactgg gagggactcg cctgtcaaag cagattcatc
     2701 cacttccaga ttgctgctgt ataccaaccg caaatcagct ggcactttat cgccggcttc
     2761 gatgattatc agatcgccgg gcacgacctc ggcagccgca atcctcttgc gaagtccgct
     2821 gcgaattacg acagctgttg gcgaagccag tttctttatc tcttcgagcg atttttctgc
     2881 ccggtattcc tgtataaaac cgagaacagc gttcagcaca acgattgcca taatggtaat
     2941 ggcatctccc attgcgccga tcagtccgga aattacggtg gcaaccagca gaacaagcac
     3001 catggtatcg gtaaactgat ccaacaaaag gaaggcggga tggagcgggg gggatgagac
     3061 aatgcgattg gggaacaatt cctggcgccg cagcgcttct ggttcggcaa gaccgttttc
     3121 agcatccgtt ttgagcagtt tggcgatctc agcaaacgtc acgttccacc aggcatattt
     3181 tgtcattttt acgtctccct tgtaaaacct tattgtcaaa tagttattcg gggaaaaacg
     3241 ataaaatgac attggacaag catttggatg aatcatacaa gaaataaaaa aaccgcagtt
     3301 atactgcggc cgttttatat attttgcgga ttttgctata tatcattgcg cattatgcct
     3361 gtgcttgcat cagcgctttt aactccaggg catgttcttc ggtaaaattg gtttcctcca
     3421 cgctgatcat gaaatgactg ccattataat taaaagcgtg gttgttctcg gcccgctcaa
     3481 aatacccgca ggatttcagg acataatctc ccacccgatt gccaaacaag atgacatact
     3541 caggctgtaa gatttcaatc tcttccatca ggtagggcag gcagttcata agcacatcaa
     3601 ccgaaggctt gattggaatt ccttcacatt ttgcattggt cagcaggcaa ttaagctctt
     3661 tgtcaatcag cttgttggac atgtaacaat tggcattttc gcgaactgca catgctttgg
     3721 gctgacagcg ggccaaaaag atgtaataaa tcttttcggt tcccagatga ttcttgatgg
     3781 tatctctgag attgagcact gcttcccgct tcatcgtaaa agggttatca tattcaaaca
     3841 ccattaacag ttccggttcc aaatcccctt ttcccagtga cggtctgcgg atgcagcggg
     3901 tcaatgactg acagcgatcg cacttgatga ttctgtcttc catattgatt atttttttga
     3961 tattctgttc cctggtcccc tttttcatca tttacatccc ccgtaaaaca gccaattatt
     4021 tcatttagaa tttctctaaa tattcccggg tacgttgatt aatcccccaa gtcgaatcag
     4081 ctatcttgct ggacatataa gcgcgtatgt tatcatccag cgcaggatct tctttgtcca
     4141 ttacgagaaa aaagcagcgt aaaatatcaa ccatattcat aaacagtcga tcagcagggg
     4201 ccttcttcag cagatgatct tttttaccat aaaaatatac catccgttta atcacccaga
     4261 caatttctgc attgctccat aaacgtttgg acaatatctc gttgagtaat tgctccgggc
     4321 cgatggtatc ttctcgcctc accagttctt tccagtcttc ttttcccaat atggtagatg
     4381 ggatcccgaa cagttcctgt tccatctggt cacctcatga ttgtcataaa tggccttaca
     4441 tcagcgcaga acccttctgg ctccgacata ggattttgcg taataggaag aactcaatgg
     4501 tgtatagatt acaccgccgc tgcggggtga actcgaatga atcatctggc cattgccggc
     4561 gtatatacct acgtgatcaa tccccttgcc gcctccggca aagaacaata aatcacccgg
     4621 ctgcagttca ctttgcgcga cagccgcacc aacactgtat tgcccggcag ctgttcgggg
     4681 aagactgtaa ccggcacggg cataaaggaa gcttgtaaag ccggagcaat caaatccacc
     4741 cgggcctgag ccgccgtatt tgtatggggt tcccagatac tgggctgcat cttccaaaag
     4801 tctggccgca ctggctgaat caccggatct tgataccggt acggcaacga ctgccgccga
     4861 agcgttgatt tttagagttt gaccaatttt cagagcatcc gtggttaacc cattgatctc
     4921 cttgatattg gcaatgctgg ttccaaactg acgggcaatg gcccagagcg tatcgccgct
     4981 cttgacaaca taaacatctg tgccggacgc agcgggtgca tttactgccg gcgcaggtgc
     5041 cggtgctggt gccggtgttt gattggcaat gctgctgagg atgagtttct gcccaatctg
     5101 taacgaatca gactgcaagc cgtttaactt tttgatttga tctatgctga ctccaaattg
     5161 attggagatt ttccacaagg aatcaccggc ttgtactgtg tagttggctg gaactgccag
     5221 tgcgattgca ctcatacatg tccataataa taatgttgct ataaatatcc cggttttttt
     5281 cttcatccta cccctacctt tcgttgaaca ttcacaataa attacttaat tcaacacagg
     5341 cagggcactt tcctgcaaac attaattttt tttcataaat tatgctattt atatttatag
     5401 gctttcaatt ttgccattaa tacagaatct attccagatc cgcccaagtg ggcacgactt
     5461 tcgctgctgc ctggaccatg gtagtcagaa cctcccgtta tcagcaactt atgttcctcc
     5521 gccagtttca agtaagcggt ggtctgttgc tccgtatgct ggggataata agtttccact
     5581 ccttcaattc ctgcttttat caggttgcca atcaaagcct ggtttttgat caaaccagga
     5641 tgagccagaa ccgctatgcc accgctcgat ttgatcaatt caatagcttc cccgggcgga
     5701 aatttatagc gccttacgta ggcgggccgc ccataaccaa tgtattgttc aaaaacttct
     5761 gccatgcttt ttgcatatcc ggcctccacc atggccatgg ctatatgcgg tcgtccaatt
     5821 agatcgcttc cagcctggcg ggatacattg gcaaagtcca gtttacatcc caaagcattc
     5881 aatttttcca cgatttttat ggcccggttt tttctttgtt cccgaatttc cgccagtcgc
     5941 tggtgaaaac cgcggttttc atgggcaata taataaccga gaatatgaat ttcgttgcct
     6001 tcttcctcgg gttcggtgtt taattccacg cctggaatat aatctatctt tattgtatga
     6061 tcggcaatgt aagccagcgt ttcctgtaat ccgtcaaggg tgtcatggtc ggtaatcgaa
     6121 atgccggtca agccaatctc catcgcttgg gccagcaagg cgggcgggct gagtgtgccg
     6181 tctgaataag tggagtgaac atgcaagtca tattcgcagc gcatcatgaa ctccttacat
     6241 taaaatactt gccaacgtat tgactacatt tcgatagaga atcttttccg tttcttcttg
     6301 actaaagcct cgttttttca tcagttgcgg caattgctga tatccgctca catctttgat
     6361 gatcatcttg tcagcgccat caaaatccga acccaatgcc acatggtcga ccccaatcag
     6421 atccgcgata taaaccaggt gatcgagcat ggctgcttca tcctgacgtt ccataccaac
     6481 aaaatcggcc acttgggtaa tcccgatcaa acccctgtgt tccgccagtt ttttcaattg
     6541 atcgtcgtcc aggttgcgcg gatgcggaca aagccggaag ctattggcat gggttgccaa
     6601 taccggttta ttgtagtatt ccagcacatc ataaaaagat gccttggcca tatgggccag
     6661 atccataacc atccccagtt ggtccatttc ttctgccagc atgcgtccca gtttgctcaa
     6721 gccgccgccg gcatcccctt cggcaacccc gcaggcaaat tgattgcggg cattccaagt
     6781 caggccgatg ctgcgcaagc cccagcgata caagagatgg agcgtgtcca gatcgctgcc
     6841 caggcattcc gctccttcca gatgcaggat tgcactgatt tttccagctt gatccgctgt
     6901 cttgatatcc tgaaagctgg taaccagctt gattttttcc tcgtttgctt ccacttcctg
     6961 ataaaaccgg ttcaattgtt ttgaaatcgc tcgtaatgcc cgatcaggtt catccggcat
     7021 cgtaaacatg gcaaaaaact gcaatccaat acctgcctgg gcagcacgtt gcaaatcata
     7081 ctgtccatga ttgcaggcaa gtcgcagttg gttttgggtg agaaacgaaa tcgtatcaca
     7141 atgcaagtcg gcaattggca aatcgattct ctcctttata aatttaatga aattagtttt
     7201 ttcgttgaag cataaacgat ggcttgcctc atcccggcaa ttaaatgatt gagaacagag
     7261 atttccagac aggcaagata aagaacctgc tgcaacgcag gttctaacct atttctttat
     7321 ctggctgatt atctgggctc aatgattaac ttgatagcag ttctctcttc gccatcaatc
     7381 ataatatccg taaacgcagg cacacaaatc aaatccattc cactgggagc tacaaaacca
     7441 cgggcaatgg caatggcctt aatcgcttga ttaatggctc ccgcaccaat cgcttgtatt
     7501 tccgcagatc ctttttctct cagaacccct gccaatgctc ctgcaaccga atttgggctc
     7561 gatttcgctg aaacttttaa tacttccatt aataaaacct cctcaatata aaaacgatct
     7621 ttattgtgat tggttgctta tatagaagta tattctcatt tctttcagta attccttctt
     7681 ttttccagcg agactatgga taaattttca tgtaatccct ttattgctta atatatgcga
     7741 aattctttcg attctgacgg cctgtctgcg atcttccgcc aattcgatca caacgccctg
     7801 caggtgagcc gggccctcgg caacctgaaa acggctgggc cgggcattta aaaaacgttc
     7861 gataatgggt tgtttttcca ttcccaaaat ggaatcgatc ggaccggtca ttcctaaatc
     7921 tgtaatgtac gctgttcctt gaggcaaaat ccgttcatcc gcggtttgaa tatgggtatg
     7981 ggtgccgaca acggcagaca ctctgccgtc caaataatag ccaaaagcta ccttttcgga
     8041 agtagcctcg gcatggaaat ctacaagaat atagtcggca gacgcttcaa cctcctgcag
     8101 cgcttcatcc agcgctcgaa aggggcaatc gagggcaggc aggaagactc tgccgctggc
     8161 attgatgacg gctattttct tgttaaatcc tgcattatat atatgcatac cctgacccgg
     8221 acaatcaccc ggataattga tcggtcggat caaacgcggc tcatcatcaa taaaagtaaa
     8281 gatgtttttg ttgtcccaca catgattgcc catcgtcaga caatcaatcc ccatccccaa
     8341 caattcgttc ttcacctcca gggtaagccc gcgcccaccg gcggcatttt caccattggc
     8401 gatggtaaaa tcaatctggt attttttctg gacccaggca agcagacttt tcaggatttc
     8461 tcttcccggt ttgcccacaa tatctccgat caccagtata ttcaaattgt gtacctccat
     8521 ttatctgtca aatacaaaca tcttgcctcg gtcgcgatac gcaaaaatgt tacgcgttaa
     8581 acggtcgggc tggttaagac tttgcagcag ttgttttttt atatcatcaa aacggttttt
     8641 cagcaagctg ttcctgtcgt tttcccggag cagcatggct tcaaacttct ggccagccag
     8701 ttgtacatat aaatcgactt cttcttcact cagcgttaac gcgtcccgcc gtaaaaccag
     8761 aataccaata ttgccttgat tgtttaattc tatacctaat tttttgggtt ccgttccccg
     8821 cagcttatgt atcatgccca gagccgcttc cagattgcgg catatcatca aattatcttg
     8881 gacgtcaata ttttctgtaa tgatcatttc gatggcgata acgttttgtt cagcctggta
     8941 ttgtatggaa ctgatttccg gattgcgaat aaaaactgat attactgcgt ctaaagctgc
     9001 ttcaatgtcc atttgatttt aatccccttg ttgcaatgta tgctttgaaa attcggtatg
     9061 tccgtatgct tttcctgccc gcctggcatg gtaatttaaa gcaaaccggt gcaaatgtct
     9121 gtatttcctt ttgtttaaca gaaaaaagaa ttgcctggat aaaatacggg aagcccgttc
     9181 aatcaacgca attccgtctc aatcacaggc ttcccgattt tttcatgtta aaacccttgg
     9241 gtttacgcag cagttattat agctgattat ttggcaaatt ccacctggcg tgtttccctg
     9301 atgaccatga ccttgatctg tccgggataa tccaactcct gttccacttt gcgggcaata
     9361 tccttgacca gggtgatggt ttccagatcg tcaatctcat ccggtttgac aatgattctg
     9421 atctctctgc cggcctgaat ggcaaatgat ttatccacgc cagcgaaaga atccgccaga
     9481 ttttcgagtt tttccagacg tttgatatag ttttccaatg tttctcttct ggcacccgga
     9541 cgcgaggctg aaatggcatc ggctgcctgt actagaacag cttcaatggt atgcggctca
     9601 atatcgccgt gatgggcttc aatcccgtga atgacgtctt tgctttcgcg atatttttta
     9661 gccagatcaa cgccgatttc cacatgtggt ccggctactt catggtcaac tgctttgccg
     9721 atatcatgca gcaagccggc acgtttggcc agtgccacat ccacatccag ctcagccgcc
     9781 atgacgcctg ccaaatgagc aacttcaatg gaatgtttga gcacattctg accatagctg
     9841 gtacgatatt ttaatctgcc cagcagtttg atcaattccg gatgcagtcc atgtaccccg
     9901 acttcaaaag ccgcctgctc gccgacttcc tttaattcct gatccacttc ttttttggcc
     9961 ttctccacca tctcttcaat acgagccgga tgaatacggc catcggatac cagatgttcc
    10021 agcgccaaac gtgcagtttc tcgccgtacc ggatcaaaag ctgacaaaat gactgcttcg
    10081 ggcgtatcat caatgattag gtcaaccccg gtcaaagttt caaaagtgcg aatgttgcgg
    10141 ccttcgcgtc caatgatacg gcctttcatc tcatcattgg gcagcgccac aacagacacc
    10201 gtcgtttccg aaacgacgtc tgccgcacaa cgctgaatcg caagggaaac gatgtttttc
    10261 gctcgtttgt cactttcctc tttcgcctct gcttccagcg ttcttatcat aaccgccgtt
    10321 tcctggcgta tttgctgctc gacattgtgt aataacaatt ctttggcttc ttccgaagtc
    10381 aatccggata aacgttccag ttccttgagt tgctgggtta aaatcaattg cagatcctgc
    10441 tgggtttttt cgatatcctt ttctttttca accagatttt gttctttctt ttcaatattc
    10501 tcggatttct tttcgaccaa ttcctctttt tgtaatacgc gtcgctcgag gcgatccaat
    10561 tctcttcttc tctcccgtaa atccttttca aattcctggc gattgcgatg aatttcatct
    10621 ttggcttcca ggaggagttc tttctttttg gcttcccctt ctttggtggc ttcatcaatg
    10681 gtccgggcag cctcttcttc tgccgacccc attttgctct cggcaacgat tttccttagg
    10741 taataaccgc cagcaatgcc aattgccaag gcaataatac ttattactaa aacgactaat
    10801 ggattgattt ttttcacctc cctttatatt cctgcatact tcatttgaaa atagaaaagc
    10861 cgagtacaaa cacccggctt taagaaaatc ctcactattt attgttttcc atggatctga
    10921 gccagctttt gctcttaaaa aaagctgtct cagttggagg ataacaaact cacctaacct
    10981 tatacgagca caaatataca atttataatg atcagcagtt cgtagaactt gctaaaactc
    11041 cgacacgccg ttcaccttgt ctgcctgaaa taaattatat atataaagtc agtgtcaata
    11101 tggtcaagtt tctaagtgag gctttgtctt ttagcctact tagattttag ttagtttccc
    11161 agagcgtgtc aagcttcctc tgtttctatc ttggcgacgg ttggaaacat tttttcccga
    11221 atcaatttct gcaaagtcag gaatatttcc ggattgtttt tcaaatattc cttggcgttt
    11281 tcccgtccct gtcccattcg ctctccatca taagaatacc aggaaccgct tttttgtacg
    11341 atgttcaatt cactggctac atccagcagg tcgccttccc gggatatacc ggttccatac
    11401 ataatatcaa attcgactga tttaaagggc ggggccacct tgttcttgac cactttcact
    11461 ttggtgcggt tgccggtaat atcattgccc atttttatcg gttcgccctt gcgaatttcc
    11521 agacgcatgg aagaatagaa tttcaatgcc cgcccgccgg ttgtgacctc cggattaccg
    11581 taaatgacgc caaccttttc acggatctgg ttgataaaaa tcacacagca agtcgatttg
    11641 ccgatgtgcg ctgtcaattt gcgcaaggcc tgggacatca gtcgggcttg caagccaaca
    11701 tgcacatcgc ccatctcgcc atcaagttcg gctttgggaa ccagcgcagc aacagagtca
    11761 ataacaatca catcaatggc attactgcgc accaaagctt cagcgatttc cagggcttgt
    11821 tccccgctgt ccggctggga gaccagcaga ttgttgatgt cgacccccaa ttgcttggca
    11881 tacatcggat caagcgcatg ttctgcgtca ataaaagccg ctatgccacc atctttttgc
    11941 gcctgggcga taacatgcag ggtgactgtg gtttttcccg atgactccgg tccaaatatt
    12001 tcgatgactc tgcctcgcgg caatccaccc actccggtcg ccagatcgag tgacatgcat
    12061 ccggtggaga tggattcaat attcatggta gtggattctc ccagtttcat aatggaaccc
    12121 ttgccatata ttttttcaat gttccggatg gcattgttta aagcctctgt cctaccctgc
    12181 ataatctcat tgttgtccac agcaattcct cctcaaacat ttgttcgttt ttatcattat
    12241 atacagactt ttcttgtctg tcaacaaatt ttatccattt aatgtatatg tatccaaaac
    12301 atgatactgt ggcccgtttc catccagcac actctgcatc agataaaatt gttcgactct
    12361 aaagctgcgc agtttattcc ggtcaggata cttttccagc agatttaaca tctctttgag
    12421 atttttgtcc gagcggatgc ggccgagtgt tagatgcagg cgatgatcct tttccggggc
    12481 aaatcccagc ggggacaaat aagcatctac ccggtcacaa agaaattcgg ttttattgat
    12541 ctctcccttg attcccatcc acattaccct tggttgcgcc tggttaggga aaaaaccgat
    12601 cccgcctgcc gatagattat gggccgggga agaatccgcc gccaaacgca gatttcgttt
    12661 gatttccggc aggtcattaa aatccacttc gccgagaaac ttcacggtta aatggtagtt
    12721 ttgatattca acccatttga tgtccggtcg ggctattccc agtttgttgc gcatcatacg
    12781 cgcgtattgc ttgatgtcat cgggcagcgg aatggctgca aaaactctca tggcaatccc
    12841 tcctgtcaat caatatggcg gcgcagcaaa tccagggccg atttggcgga caacctgcga
    12901 acgatttcgc gggacccgcc aagttttaat tccctgacac ggcacgtttg tgcccctgcc
    12961 agggcaacgt ataccaagcc taccggtttt tcccgggtgc ctccgcaagg gccggcaatc
    13021 ccggtaatgg ccaggccgta atcaactcct gacaattgcc gtatgccccg ggccatttct
    13081 tccgcggtct gttggctgac ggctccgtat tgttccagag tggatgcttg tacgttgaga
    13141 agtttcattt tgctgtcatt gctgtaagta atgacccctc cccaaaagta atccgaactg
    13201 cctgaaactt cggtgatcat gctgcccagc ataccggccg tacaagactc ggctacggcc
    13261 agtttccgac cccgggctgc cagcagtttg ccggtttgtt cgggcagggt ttcatcatca
    13321 tagccaaaga tatgtttgtg cattttgtgc tgaaccaatt tcgtcaattc attcaggcgc
    13381 ggtatactgt cggttgattg cccgcccgcg atggcaagct tgatatgaat ttcgccttcc
    13441 tgtgccagca aagccgccga atagcctgat gaattattca gcatatcggc cagcatctct
    13501 tcgacttggg attcaccagg tcctatgact ttgatggtcc ggcgcagcac tttttgcagt
    13561 tgatcagcat aatctctttc cagacgaggt ttgacctcac tcaggtacat cctggtcatt
    13621 tcccgcggtg gtcccggcaa cagaatcagc agcttgtttt gtcggcgcaa atacatgccg
    13681 ggggcggtac cctgggtatt gctcaaaact tctgcttcga ctggaaacat ggcttgcttc
    13741 atattgattt ccggcatggg acgccgccgt tgggcgaaaa aatcccgcag ccgctgttct
    13801 tcctcaggtc tgaccaccag gctgcatccc attaactcgc aggccactgt cttggtcaaa
    13861 tcatcaaagg ttggtcccaa gcctcccgtg gaaactatga tatccgccgt ttccaatccg
    13921 gcagcaaagg ccatttggat ttgttcgcgg ctgtccccaa cggtaacctt gcccgcgact
    13981 ctcaggccaa tgtcctccaa ttgctgggcc agatattcgg aattgctgtc gtgggtggtt
    14041 cccagcaaca attctgttcc ggtggaaatg ataaatactt ttggcacgaa ccccactcct
    14101 tgttcattcg caaatcaccc gattattcat atagcttagc atagacttga cgattgtcgt
    14161 taatacattt ttagttgttg acaagacagc aaaaaccgga cccaggtccg gttttcattt
    14221 actgttcatc tgtttttacg aagcatacag aatcttttcc aggcttgccc tgcccaggct
    14281 ttcctgctgg cgcagaccgg atgccaggag aatcaagcgc tcccgattgt cttgcagcag
    14341 gtcgcgggtt ttattttcag cggctgtaat gatgcgtttg ccttctttat ataccacttc
    14401 agccggaagg ctgtcatcgt ccactacccc cagggaagac aacccactgc tgatcatctc
    14461 ccgggccacc tgccaggctt gctgaaaatc attgcgggcg ccgctgctgc ggctggcaaa
    14521 tgcgatttcc tctgccagcg ccccggccag catgatcatg atctgttgtt ccagttcttc
    14581 atacgtgtac aaatactgat catcctgcgg tgagcggcgc atatacccca gcgctttgcc
    14641 gcgcggaatg atggtcaggg aggcgaccga accctcctcc aggtattcac acaccaaggc
    14701 gtgtccgctc tcatggatgc tgattctttc catttcactg gatgacggct gacggtcaag
    14761 cttttcaccc atcatgactt tatcgatggc ttcgcgaaaa tgggtttgct gaatcagggt
    14821 actgttttcc cgcattgcca atatggcagc ttcattggtc aggctctcca gatgggcgcc
    14881 ggaaaaacca aaggtggccc gggcgatgtc atccagatca acagtcggat ccagcggctt
    14941 gttacgggta tgaatcgcca ggatcttgcc gcgtccgcag cgatcaggca aatcaacctg
    15001 aacctgccgg tcaaaccgtc ccgggcgcag aaccgccgga tccagcaggt cgggccgatt
    15061 ggttgcccca atcaacagaa tgcgggggtc acggttttgg gtcagcccat ccatttccac
    15121 cagcagttga ttgagggttt gatcgtattc catgtgtccg ctgtgacttc cccgtttggc
    15181 cgccaacact tccagttcat caataaagat aatggcactt tttttattta attttcgggc
    15241 ctcctgacga gcggaggcaa agatctgccg aatccgtttg gcacccaccc cggcatacat
    15301 ctcaataaat tcactgccgg aagcagccac aaaagctgaa ctggtaaaac tggcagcggc
    15361 cctggccagc agcgtcttgc cggtcccggg cggtccggcc agcaggatgc ctttcagtgg
    15421 cctgattccc atatgatcga ttttttctgg ttgcatgaca aactgcaggg cctcctgcaa
    15481 ttcattgatg gcactgtcct gtcctccgat ttcggtaaac tcaagcacgc tttgcttgtc
    15541 ccgccgctcg tcgctgttgg acaatttcat cctgccctgt cccggcaaca gataaagcac
    15601 ggcaaagatc agggcggcgg cgacgatgac cggggtcata ttgaagccat tcagccacaa
    15661 gaagatcatt atgcccagac cggctccaat caaaatagat ttcatcatga tttctcccca
    15721 ttcgctgctt caatactgtc tggaggttgg cgctcaataa tttcgtacag gaattgttcg
    15781 ccgcgctgaa ggtgcacaaa aagatgctgc gtatcgattt gcagttgaca agtaaaacca
    15841 ttacgtgcgg cgaccgcatt caggtgctgc tccaaagcaa catactggtt ggaggcaatc
    15901 gcttcataca aggccaattc caactgctcc cattcatttt gcagcgcggc attacgctgg
    15961 tcgtcaagca caattccata atttccgcgg ggaaatttcg cggccattat ctgatcgatc
    16021 tcattgtaag cttgtttcag gttgtccact gattgcatgt gaacgttgat ggtgttgcgg
    16081 ccctggtcat tacacagcac tgcctcatcc acacaaggca gcgccagcag ttgtttcacc
    16141 accggttttt ccaccatgtc agcccgatat attttttgtc cgccgaccag caatcccagt
    16201 gtaagcaaca accccagcaa caccagggca accttgattt catggttttt catcaccata
    16261 ccacctttac gaacccatta tatcacgtga atagccgtaa ttatgaggtt caatttatgg
    16321 caaggcaggc ttttgcaggc aatcgatcag aaaacaaaac gatacatcag ccggttagcc
    16381 aatgtatcgt aaataagccg ggtttattgg ttttggtctt cactgctccg aaatttcata
    16441 aaataatcca gaccggatat aacggtgatg gccagggcga tgtaaatggc ccatacgccg
    16501 atgatatgca gcggcgggta aagactttcc agaataatga tgatgaccgc cacaatttgc
    16561 gatatggtct tgaatttgcc caatttgctg gcgggtatga tgatgccttc ttccgcccgg
    16621 acagctctca gccctgaaac ggcaaattcc cgtcccagga tgacgatagc gatccaaccc
    16681 tgcagtctgc ccaattccac cagacagatc agggcggctg tgatcagcag tttgtcagca
    16741 attggatcca gaataatccc taatttggta acctgcttgt acttgcgggc caggtaccca
    16801 tcgagactat cggtcaacgc agcagccaca aatataatgg ccgccagtaa atcaccgtaa
    16861 gggatatgta atactaatac caccacgaat accggtacca aaaagattct caggagggta
    16921 aggctatttg gtaaattcat ccacgatttc tcctatcaag tcataattcc gtatggcttt
    16981 taattcgacc ttgataaatt ctcccttggt cagcctgcgt tcacttttga ccatggtaat
    17041 tccgtccaca gcgggagctt ggtaatatcc cctgcccaga taaagatgcg gggataactg
    17101 tgatgatata agtatctcat aattttgctg caatcgcttt ttatttttct tacgggtgat
    17161 ttttttttgc aatttcataa tggaggccag tctggcttgt ttgatttcct ccggaacctg
    17221 cccggccaag ttggcggcag gtgttcctgc ctcgggtgta aaggcaaacg cgcccagcca
    17281 gtcaaactcc gtttctccca gaaagtcata caggatttta aaatcatctt ccgtctctcc
    17341 gggaaagccc aacatgactg tcgttctgag gaccaaacca tcaacttggg cacgcagacg
    17401 agagaaaagg ttttttagat cggccgcgtt atgtttacga ttcatggcct taagcatggg
    17461 gtcggcagca tgctggatgg gaatatccaa ataagggaga accttgtccc cgcccgccac
    17521 ggtatcaatg atacgctggt caatatgggc gggatgcagg taaagcaaac gaatccacgg
    17581 gagttcttcg attttgctga gttgctgaag cagtccgccc aaatcgcacg tgcccgacag
    17641 atccttgccg tataccgccg tatcctgacc gatcagcacc aattccttga tgccctttcc
    17701 tgccagatca cgggcttcgg caagcagttc gccagccggc cgggaacgga gattgcctct
    17761 gatgagtgga atggcacaat agctgcagcg attgttacag ccttcggtaa tttttaaata
    17821 ggcagaaccg gggggagttg tcaatacccg gggacctttt tcaataaata atggcggagg
    17881 cggctcgatg gccagcagtc tctctccctg gaggatgcgc tcaagatgct ggacaatcgc
    17941 aggaaaggtg gagatgccaa tcagaccgtc caattcaggc agttcgtcca gcaattcctg
    18001 tccatagcgc tgggccagac agcctgtaca gaccaggtat tgcaacattc cccgttcttt
    18061 taactgtccg gcttcaataa tcgcattaat ggattcctgc cgggcatctt caataaaccc
    18121 gcaggagttg agtatgacta tatctgctct ttccatattc gaaacgattt tgtacccggc
    18181 acttttcaat ctggcaatca taatttcact atcaacttga tttttggcac atcccagact
    18241 gataaaaccg attttgtatt gatggttctt aatagttgtc actgttttta aattcctttt
    18301 ccacgacttg ccccatctcg cccaatgcag caacgggttt gccattcacg gtgacatcaa
    18361 ctgcacctgc attgccgagt ctgagttgaa ggtttgctcg ggctgtaaac gtctcttcct
    18421 gctcttctgc cagcgtcatt tcggttttgt taccaccatc cgtcacaact cttatccagc
    18481 attgcatgcc aggtttgacc ttgacccgga tttccatagg cacagcggca ggtgcttcag
    18541 actgatctgc agccggtgga ttcttttccc cgctttgaac cgggggggcg ggcggtgcgg
    18601 gcgtcatttt gtcacccaga tagttggcaa acaaattgcc aacccaaatg gccagcagta
    18661 aaaacaccac cgccccggcc agattgcggt aaggcaaccg cggcaactga atcttcctgc
    18721 gctttctgac cggtttttca gcaaccggtt ccgggatgac ttcgggatag gcaacttgtt
    18781 tgaattctgc gcaaacgagt tcttcgtcca gctttaatac gcgggcatac tgttttacaa
    18841 agccgattgc atacacctgg ggaggcaaaa tggcaaaatt ttcattttcc aacgcttcaa
    18901 tatataattt gcgaatcttg gtttcggcct caacctcatc cagggtaatg cctaaccatt
    18961 cgcgggttgc gcgcagtctt tcaccaaatc cgtccaactt tgctcacctc aattacccat
    19021 cttcttgaat aacaaccaag caaaatgaaa tccctatctt tcatctaata atgatcaaat
    19081 ttcatttttc gaagcactgg cagtggctat cctgatggcc tgacgcatcg gatatcccgc
    19141 tgccagtcca taaaccagac ctgccagaaa cagatgcaaa tcattgc
//
LOCUS       DAOTHZ010000039        19069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3467_length_19069_cov_7.686494, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000039 DAOTHZ010000000
VERSION     DAOTHZ010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 19069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19069
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_19069_cov_7.686494"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..563)
                     /locus_tag="P5549_06085"
     CDS             complement(300..563)
                     /locus_tag="P5549_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007777897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW12677.1"
                     /translation="MTKTTEAITFELEKATFNIFPEYCKGCGLCKEKCPNQVIVWSDT
                     LGVYGTPTVKPKDEDSCIACSICEMVCPDCAISIIKKKTRKAI"
     gene            complement(556..1143)
                     /locus_tag="P5549_06090"
     CDS             complement(556..1143)
                     /locus_tag="P5549_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRW12678.1"
                     /translation="MALFKIALAGEGGQGVQSIAEILAEAAYNENQQAIYIPNFGLEQ
                     RGGVSIAFLQFSDKRIGAPKFQKADIVIALSERAVARTLGYSGTETLYVYDSSFNIKT
                     EELPQEAKQIIALPAIDTANNELHPRVFNIIIMGAVIGLSNVVSFEAAKEALEHKLGY
                     KFEKDPKLRELNFKALEIGRQMAEKSLREGVLSND"
     gene            complement(1143..1892)
                     /locus_tag="P5549_06095"
     CDS             complement(1143..1892)
                     /locus_tag="P5549_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRW12679.1"
                     /translation="MAVQAIPKSWYLPSKPHKFCPGCGHGIVLKALGEVIDELEIQDQ
                     VIFGCDIGCSLLSWNFFACDSTQTHHGRTTPVMVGIKRSRPELVCIGYMGDGGGYSIG
                     SQHLLNSAVRNEKVTLILVNNTNYGMTGGQMAPTTLPGQKTETSPYGRDPEQTGYPTM
                     GPEFIAQLTPDSAYVARATVSKYKQLKKYIRSALRNQMEGNGFSFVEVLSTCPTNWRT
                     NAQQTWNFLEDEMERYFPVGELKNPFGKGDK"
     gene            complement(1908..2996)
                     /locus_tag="P5549_06100"
     CDS             complement(1908..2996)
                     /locus_tag="P5549_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HRW12680.1"
                     /translation="MQRVIGESKKSFMTGNEAAAWACLAAGADIMFGYPITPQNEVMH
                     YWTRMAPKYGKGFLQTEDELSAGFTVCGGVQSGKKAFTATAGPGNVLMQEPFGMAEAM
                     RLPLLAIIQQRGGPSSGTVVYSQQEVTLTTYGGNGEGHRIVYSTSSHQEIFDYIIKGF
                     NNAWKYRFPVFILGDGYQAKMREPLEMYDPETRGIEMVEPYPLIGLGGKIGEERGPSH
                     LCNIYSLEEELYEVITKVQAEYDALAPQIIEYDEKNCEDADIIIVTHGIVSRAADDAV
                     KALRAQGIKAGYFRPITLRPFPQKELREAIAKNGAKKLIIAESAIGQLERLVKQEIYG
                     ETAKIETLFMPGVGIIDSDIIDKVKSVL"
     gene            complement(3043..3720)
                     /locus_tag="P5549_06105"
     CDS             complement(3043..3720)
                     /locus_tag="P5549_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12681.1"
                     /translation="MIFTFKEDARIVEAYIGEYASGKSEMAINRALELSRQGRQVTLV
                     DLDTVEPFYTLRPIKHQLEKMNLNIISQSREDSFGLGETGSMLNPRARWALRNKGDII
                     LDIGYGVFGARTLNLVEDVDQTPELKVIAVLNASRPMTNSLDRILEYVGGLGRVDGIV
                     ANTHMGDETTPEIINTGNEIIAEAARILHLPVIYTAVVEDFKDSKINDFGMSIKYIKR
                     YMPSAIW"
     gene            complement(4028..4291)
                     /locus_tag="P5549_06110"
     CDS             complement(4028..4291)
                     /locus_tag="P5549_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HRW12682.1"
                     /translation="MATANLVMAILFLLFIVFIIAQLIMKPLKLIWKLVINSVIGLAL
                     LVLTNYVGAYFDFSLPLNVLTVLIAGFLGIPGILLLICFQLLL"
     gene            complement(4292..4567)
                     /locus_tag="P5549_06115"
     CDS             complement(4292..4567)
                     /locus_tag="P5549_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12683.1"
                     /translation="MCNLIKFKSCRLAASKPHQLYADKGIDLATVKEAGNELRLAYQI
                     LSQADEEDMVDYAVFTVKAAEKRYGYLLKKYKQQEKQELLRTKGGNI"
     gene            complement(4635..5231)
                     /gene="recR"
                     /locus_tag="P5549_06120"
     CDS             complement(4635..5231)
                     /gene="recR"
                     /locus_tag="P5549_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRW12684.1"
                     /translation="MRLARPLENLIDRLQKLPGIGPKTAQRLALFILKMPVDEARQLA
                     QSIIVAREKVFPCPHCGFLTDENPCLICRDTSRDRSLLCVVADSSDIIALERSGYRGQ
                     YYALNRGFNLMDDIDLGDINLQPLQQLLAREKINEVILAFNPDMDGEVLSRYLAVSLH
                     RQGIKVTRLAHGLPVGGDIEFADEITLRMAIEGRKECI"
     gene            complement(5252..5581)
                     /locus_tag="P5549_06125"
     CDS             complement(5252..5581)
                     /locus_tag="P5549_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRW12685.1"
                     /translation="MKFNPGGGANMNQMIKQAKKMQEQMAKMQSELQEKVVEASAGGG
                     AVTAKVNGRQELLEIKIKPEVVDPEDVEMLEDLVLAVINDAIKQSQDMVSGEMSKITG
                     GLNIPGF"
     gene            complement(5597..7090)
                     /gene="dnaX"
                     /locus_tag="P5549_06130"
     CDS             complement(5597..7090)
                     /gene="dnaX"
                     /locus_tag="P5549_06130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRW12686.1"
                     /translation="MAYLALYRVWRPQTFIEVVGQEQTVIALQNAIREDRLTHAYIFS
                     GPRGTGKTSIAKIVAKAVNCEHPVDAEPCNQCSACTDINNGNFMDVIEIDAASNRGID
                     EIRDLRERVRVMPAQGKKKVYIIDEVHMLTAEAFNALLKTLEEPPDSVIFILATTEPQ
                     KIPSTIISRCQRFNFKRLQSDQIIARLRDAANRFGIVMEEEALALIARRANGGLRDAL
                     GMLDQVYSFKEKDISKENVLEVLGLVDDNFVADIFDAVLGKDPGTLVHKIHISVQQGK
                     ESTQLVRECSLYLRDLLMAKLIGDKAELLMVSEANRDRLIQQTQLANKDQILQLLRMT
                     MDLAEKLRFSEGQRFLLEITFLEMIAVISGQTSKPEASPEPRKKDTTTSKDKPVKNDA
                     REILWNRILLGVKEKKVPTHALLAQGKLLGTKGDTMYIGYTKGYRFHKERMEEKANRE
                     ILDQVIKEIFNREMEIQFLFLDEKQTNDIVVQKAIEYFGKENIEIKD"
     gene            complement(7194..7459)
                     /gene="ffs"
                     /locus_tag="P5549_06135"
     ncRNA           complement(7194..7459)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5549_06135"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(7651..8157)
                     /locus_tag="P5549_06140"
     CDS             complement(7651..8157)
                     /locus_tag="P5549_06140"
                     /inference="COORDINATES: protein motif:HMM:NF021120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HRW12687.1"
                     /translation="MNLTKKLLLTMMVCAIGFSLLLAGCQTSQKPTTPQPNPPTTQKP
                     YMTSPDNSMTSTTAETRQIAMKAEKEAEKADGVRDATALVAGKSMYIGLDLEADLGQD
                     KADMTENSVLNNVKNMDPSYKIMVSSDIDTVTRIKRVAQGIAAGRPLTSFSNEIKDIG
                     TRMTPKSK"
     regulatory      complement(8382..8464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9140..10363)
                     /gene="nuoF"
                     /locus_tag="P5549_06145"
     CDS             complement(9140..10363)
                     /gene="nuoF"
                     /locus_tag="P5549_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRW12688.1"
                     /translation="MAEEMRIVLRNVGKIDPLKIDDYINAGGYKSLAKAKSMDRKALI
                     DEVKNSGLRGRGGAGFNCGMKWSFSYMLETDQKYVVCNADEGEPGTYKDRIIMMEDPQ
                     SLFEGMAICGYAIGSNKGYIYCRGEYPYVVERLNQAIAQAKVKGVLGDFDIEVRMGGG
                     AYVCGEESALIESIEGHRGEPRFKPPFPGVEGLWGKPTIVNNVETYANIPAIVEKGAD
                     WFKNIGAASYPGTKILTLTGDVVNRTYFEVPTDVTIRDVIFGLGGGIVGGKNFKAVQI
                     GGTSGGFIPEQLLDTPIDFDSMAKIGATLGTGAVFVMDETRDIVDIVDRISKFFEHES
                     CGKCNPCREGTKRMHEIMHKINAGKGTVADMDLMENLGGVMCVACLCGLGQAAPAPVL
                     TTLKHFKAEYAAKMN"
     gene            complement(10385..10831)
                     /locus_tag="P5549_06150"
     CDS             complement(10385..10831)
                     /locus_tag="P5549_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRW12689.1"
                     /translation="MADYKSIIDAYKDLPGGIIEAYHALQREFSYIPEEAIPYAAQVF
                     GVSKPRAHGVATFYSYLRVGKRGKNVIRICESAPCHIAGADKVVAALEKELGIKMGES
                     TSDGKFTLEFTECVGQCQATPVITINSKPYGDVTAEKIPAILAEYK"
     gene            complement(10831..13527)
                     /gene="fdhF"
                     /locus_tag="P5549_06155"
     CDS             complement(10831..13527)
                     /gene="fdhF"
                     /locus_tag="P5549_06155"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(12469..12471),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HRW12690.1"
                     /translation="MELVKLTINGKEVEVPRGSTILQAAESNGIEIPTLCYLKDLSPM
                     GSCRMCVVEVKGNRLLPASCVTPVAPGMEVQTESPAVINARRTILELLIANHPLDCLT
                     CDKLGDCKLADYCYQYGVKQSPFSGEQHHYAIDDSNPFINRDMNKCIQCGACVRACEE
                     LTGQDNLSYLHRGFHRKVTTYGDVDYMNSDCVFCGQCVAVCPTGALTEKTMAGQARRW
                     EIEKVRTTCPFCGTGCTFDLAVKDKKVIGVLSSPDATVNGRSLCVKGRFGWDFIYNEK
                     RLTSPLIKKNGKFEEASWDEAYDLIAKRFNEIKNKYGSESFAALSSARCTNEENYLVS
                     KFTRAYMGNNNVDHCARTUHAPTVAGLATTFGSGAMTNPISDITNEAELMFLIGTNAT
                     EAHPVIGYKMRQAARNGAKMVVCDPRHIDLVDEADYWLRQKPGTDIPLLNGLMHIIIK
                     EGLEDKKFIEERTENYEELKATVAEYTPERVSELTGVSVDDLYAVARLYATTSKAMIF
                     YTLGITEHICGTRNVMSCANLAMLTGHLGRPGTGVCPMRGQNNVQGACDMGALPNVYS
                     GYQNVTVDAVHEKFEKAWNAKLSNKVGLKIPEMFAGAHEGKVKAMYILGENPVLTDPD
                     ANHIRGALEHLEFLVVQELFLTETAEYADVILPAASFAECDGTFTNTERRVQRVRKAI
                     EPIPGQANWQTICEMSTRAGYPMSYNHPSEIWDELASLSPSMNGINYDRLEELKSLQW
                     PCPSVDHPGTPFLHAGKFTRGLGLFQPSEHIPPGEMPDEEYPFLLSTGRILYHYNVTT
                     PYSKGISSVWDKEMAEVNPVDAEKLGLVTGSKVRVTSRRGVADTHVKVTDRVLPGVIW
                     MSFHYNATPTNALTSHHLDPITGTGEYKVAAVKIEKLQEVNQ"
     regulatory      complement(13637..13756)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(13941..15140)
                     /locus_tag="P5549_06160"
     CDS             complement(13941..15140)
                     /locus_tag="P5549_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRW12691.1"
                     /translation="MLVNISLEEAYNQILQYCQPLDAESIPIMTALGRVSAVDLHAPA
                     DLPVRQQSAVDGYALCAVRTEYRLIGKGKPGEIPTELLLPGQALAVCTGGNLPGGTTA
                     VIPHEKTEISGQSLYPREEIKPGNNIKQAGEDFPADSLMASQGSIVGAGEIGLLAAFG
                     MAAIEVYRRPRVAVLCLSENVVEWQKNPQPGQMRDSNSPMLSSLVIRDGGIPVGGCTS
                     DNIMDSLQEAAERLLGQCDILIITGGTYADGESEAASLMGKLGAEIIFWDVSVQPGSH
                     IGFALKHSKQIFALSGNPAACSVGYHLFVAPALRGMQGYDPGYKRISAICSNGFPKKA
                     GTRRFVRGQLTWTEKGWQITVLPGQKPSMIRSLLQCNALIDLPAGSPPVDAGQEVVLI
                     VLESVKI"
     gene            complement(15160..16191)
                     /locus_tag="P5549_06165"
     CDS             complement(15160..16191)
                     /locus_tag="P5549_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRW12692.1"
                     /translation="MRKVPVDEAVGLTLGYDITRIVPGREKYRAFRRGQVISPEDISK
                     LKDIGKEHIYIWDPDDDLVHEDDAALHLAQCAAGAGLYWGEPNQGRVNLKAEYAGLLK
                     VNADALHMLNNLEDVIFATLHNDRVVVEGQTVAGTRVVPVAVEQKLLQEAKNICAGKN
                     PILSVKPFKPLWTAVVTTGTEVNSGRIKDGFAGVIRNKIAPYSGRWMGQVIVPDDPEL
                     ISREVHNFVAEGAQLVIVTGGMSVDADDATPQAIRKSGAEVVFYGAPILPGSQFMLAY
                     QGRVPICGVPGGALFSRKTTLDLLLPRIFAEDKIERSDIVAMGHGGLCEECKQCHFPQ
                     CPFGKASIF"
     gene            complement(16370..17434)
                     /locus_tag="P5549_06170"
     CDS             complement(16370..17434)
                     /locus_tag="P5549_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HRW12693.1"
                     /translation="MGLYIDIKKKLSGFTLQIKLSSEQEIIGVLGASGAGKSMLLHCI
                     AGLVRPDEGQIIVNGKTYFDAAQKINLPARYRKTGFLFQNFALFPHLTIEENIAFGLD
                     KFPKTDQQQKTFALMKRLNITDMAKRYPSQISGGQQQRVALARALAVEPEVLLLDEPF
                     SALDNHLKNHLMKDMQLTLKEFKGNALFVTHNMEESYRLCDHIAVLNAGQVETFASKE
                     EVFLHPVSLETAQITGCKNIAAAQCKSTYTAEVPAWGITLHTDTSILSPNGFVGIRAN
                     HIMFAESAGNNCFPVWIADESRSPFRTTLYLKIGSAANGTDDYHIQWEISREQREKIS
                     KLQSPFYVFMDPGRVFFVEN"
     gene            complement(17436..18122)
                     /gene="modB"
                     /locus_tag="P5549_06175"
     CDS             complement(17436..18122)
                     /gene="modB"
                     /locus_tag="P5549_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRW12694.1"
                     /translation="MHFDWSPAFISIKITLIATIIIFFLGLAAAWWMVNYRGRWQGLL
                     DGILTLPLVLPPTVAGFGILLLIGKNGPIGKLLGMFDINIIFTWHAAVIASIVVAFPL
                     MYKTARGAFEQIDPNILNAARTLGVSEWKVFWRVSVPLARPGIAAATALAFARSMGEF
                     GATLMVAGSIPGQTQTIPIAIYFATLGGEMRVALFWVLVILSISLTVIVIMNNWNNAQ
                     RKLKLGAGGK"
     gene            complement(18151..>19069)
                     /gene="modA"
                     /locus_tag="P5549_06180"
     CDS             complement(18151..>19069)
                     /gene="modA"
                     /locus_tag="P5549_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRW12695.1"
                     /translation="WDGGSNTATINGTIQIKVGSNEITTSYGTIGMDTTAFIKNGRLY
                     IPVRSVANAMGYGVSAANQNGVVTANITTKVDLTISAAASLKDALTDVQSLYLQEKPK
                     TTLTINFAGSGTLQKQIEQGADVDLFFSAAASNMDVLKTKDLLIDSTIRNVLGNKLVL
                     VVPNDSTVPVNSSFSVVVTDPSVKKVALGEPKTVPAGKYAENVFTFLNILDKVKEKVV
                     YAQDVRQVLNWVETGNVDAGVVYLTDAKISAAVKVIATASEESHTPIVYPAALIKSTN
                     NYTASRDFLNFLTSDKAKAVFDKYGFEVL"
BASE COUNT         4806 a         4820 c         3879 g         5564 t
ORIGIN      
        1 tctctttgtt ctgttttatc aagatcctcg cgcttccctc tcgttccgcg caagaattat
       61 aataacacct gaacaatttt atgtcaagat tgataacacg gttttttcga taacttatta
      121 tattttcttc agtatttatg tgtccgtttg tctggaagca acatattgaa ttgaccgatt
      181 tgttttgtaa caattttatt gttatttcta tatataaata gtccttcacc gattatgaac
      241 aaattaaccg attaaaaaac tgtccccaat ctttggggac agtttgtttc ggactagcct
      301 tatattgctt ttctggtctt tttcttgata atggatatgg cacagtcagg acaaaccatt
      361 tcacatatgc tgcaggctat acaactgtct tcatcttttg gttttaccgt aggcgtacca
      421 taaacgccaa gtgtatctga ccagacgata acctgatttg ggcatttttc cttgcataat
      481 ccacaacctt tgcagtattc cggaaatata ttaaaggttg ctttctccag ttcaaaagtg
      541 atggcttcgg ttgttttagt cattgctgag caccccttcc ctgagggatt tttcggccat
      601 ttgccgtcct atttccagcg ctttaaagtt taattctctc agttttggat ctttctcaaa
      661 cttataaccg agtttatgct ccaaagcttc tttggccgct tcaaagctaa ctacattgct
      721 taacccaatt accgcaccca taataatgat attgaatacg cggggatgca gttcgttgtt
      781 tgccgtatca attgccggca aagcgatgat ttgtttggct tcctgcggca attcttcggt
      841 ttttatgtta aaactgctgt catatacata aagggtttct gttcctgaat agccgagggt
      901 tctggctaca gccctttcgc tcagggcaat cacaatatct gccttctgaa atttgggagc
      961 tccgatccgt ttgtcactga actgcagaaa agcgatggac actccgcctc tttgttccaa
     1021 tccgaagttg ggaatatata tggcctgttg gttttcattg taggcagctt cggccagaat
     1081 ttcagcaatt gattgtaccc cctgtccgcc ttcgcctgcc agagcaattt taaatagcgc
     1141 cattatttat ccccctttcc aaacggattc tttaattccc ccactgggaa atatctctcc
     1201 atttcatctt caaggaagtt ccaggtctgc tgtgcattcg ttcgccagtt ggtgggacaa
     1261 gtggaaagaa cctcaacaaa ggagaagcca ttcccctcca tctggtttct caaggcactc
     1321 ctgatgtatt tttttaattg tttgtatttt gaaaccgtag cccgggctac ataggcacta
     1381 tccggagtca actgggcaat aaattccggt cccatggtag gatatccggt ctgttcggga
     1441 tctctgccgt agggagaagt ttccgtcttc tgccccggca gcgtagtggg ggccatttgc
     1501 ccgccagtca taccgtaatt ggtgttgttg accaaaatca atgtgacctt ttcatttctg
     1561 actgctgaat tcaataaatg ctgggaaccg attgaatacc ccccaccatc acccatgtaa
     1621 ccaatacata ccagttcagg ccgggatctt ttaatgccca ccataaccgg agtcgttctg
     1681 ccgtgatggg tctgggtact gtcgcaggca aaaaaattcc aggacagcag cgaacatccg
     1741 atgtcacagc cgaatataac ctggtcctgt atttccagtt catctattac ttcacccagc
     1801 gccttgagca caataccatg tccgcatccg ggacagaatt tgtgcggttt actgggaaga
     1861 taccatgatt tcgggattgc ttgaacagcc attgttttac ctcctttcta caagacagac
     1921 tttactttat caataatatc actatcaata attccaacgc cgggcataaa cagcgtttcg
     1981 attttggctg tttctccgta gatttcctgt ttgaccaaac gctccagctg tccgatggca
     2041 gattctgcga tgataagttt cttggcaccg ttcttggcaa tagcctcccg cagttccttt
     2101 tgcgggaagg ggcgcaaggt tatgggtctg aagtatccgg ctttaattcc ttgggcacgc
     2161 aaggccttca ctgcatcatc tgccgcgcgt gatacaatcc catgggttac aataataata
     2221 tctgcatctt cacaattttt ctcatcatat tctataatct ggggggccaa tgcatcgtat
     2281 tcagcctgca ctttggtaat tacttcatat aattcttcct ccaggctgta gatattgcac
     2341 agatgactgg gaccgcgctc ttctccgatt tttccaccca agccgatcag tggataaggt
     2401 tcaaccattt caatgccgcg cgtttccgga tcatacattt ccaaaggttc gcgcatcttg
     2461 gcctggtaac catcaccgag gataaatacc ggaaatctgt atttccaggc attgttaaaa
     2521 cccttgataa tataatcaaa aatctcttga tggctggagg ttgaataaac gatacggtgt
     2581 ccttcgccat tccctccata agtggtgagt gtgacttctt gctgcgagta gactacggta
     2641 cctgatgaag gacctccccg ctgctgtatg atggccaata atggcaaccg catggcctca
     2701 gccatgccaa agggttcctg catcagaaca ttccctggtc cggcggtcgc ggtaaacgct
     2761 tttttgccgc tctgtacgcc gccacaaaca gtgaatccgg cggaaagttc gtcttctgtc
     2821 tgtaaaaatc ctttgccgta tttaggcgcc attcttgtcc agtaatgcat tacttcattt
     2881 tgaggagtaa taggataacc aaacattata tctgcgcctg ctgccagaca agcccaggcg
     2941 gcggcttcgt tgccagtcat aaatgacttt ttgctctcgc caattactct ctgcatttat
     3001 ccaatgcacc tcctataatc ggatttgtta attgattcat cactaccata tcgctgatgg
     3061 catgtagcgt tttatatatt ttatcgacat accaaaatca ttgattttgc tgtctttaaa
     3121 gtcctcaaca acggcggtat atataactgg caaatgcaga attcgggccg cctcagcaat
     3181 gatttcattg ccggtgttaa tgatttcagg agtcgtttcg tctcccatat gggtattggc
     3241 tacaatcccg tcgactcttc ccaatccgcc aacatattcc aaaatcctgt cgagcgaatt
     3301 ggtcatgggg cgtgaggcat ttagaactgc tatgactttt aattcaggtg tttggtcaac
     3361 atcctccacc agattgagcg ttcgtgcgcc aaatactcca taaccaatat ccaggataat
     3421 atctccttta ttgcgcaaag cccatcttgc cctgggatta agcatcgaac ccgtctctcc
     3481 cagcccaaag gaatcctccc gggattgact gatgatatta agattcatct tctccaattg
     3541 atgctttatg ggtcttaaag tataaaaagg ctcgactgta tccaagtcga ccagagtaac
     3601 ctgtcggcct tgcctgctca gttccagagc cctgttgatt gccatttcgc ttttgccgct
     3661 ggcgtattct ccaatatatg cttcaacgat cctggcatct tccttaaacg tgaatatcaa
     3721 cacctcctgc gttatcgcgg ggcaaatatc aaatatttat gccttgtccg tttttttgat
     3781 agctctttaa tgtagctttt ctaagtataa attagtcatc aatgtgcgtc aatattaaag
     3841 ttcctttgat taaataatac gcacatttat gtattgttca tcataaagct ggtatgtttg
     3901 gccattaatt gtccgtatta agtacttaaa gagtaccctg tctatagtaa tattccacat
     3961 ttttattcat tcactcaaaa aagacggatc tcctccgtct tttcgctgta taacattttg
     4021 ctgtcaatca cagcagcaat tgaaaacaaa tcagtaataa aatgcccggt attcctaaaa
     4081 agccggcaat taaaactgtt aatacattga gtggcagact gaaatcgaag taagccccga
     4141 cataattggt taataccaac aaggccaacc caatcaccga attaataacc aatttccata
     4201 taagtttcag tggtttcata atcaattggg caataatgaa aacaatgaac aacaaaaaca
     4261 ggatcgccat tactagattt gctgtagcca tctagatgtt tcctcctttt gtccgcagta
     4321 attcttgttt ttcctgctgc ttgtactttt ttaacaggta gccgtatctc ttttctgctg
     4381 ctttgactgt aaatacggcg taatcaacca tatcctcttc gtccgcctgt gacagaattt
     4441 gataggccag tcgtaattca ttgccggctt ccttcactgt tgcaagatca attcctttat
     4501 ctgcatacaa ttgatgcggc ttgcttgcag ctagccgaca ggacttaaat tttattaagt
     4561 tacacacggc ttcaacctcc tggcaataat agcgatgatt tccatttcat tgcatattat
     4621 gccattcagc tgccttatat acattctttt cttccttcga tggccattcg caaagtaatc
     4681 tcatccgcaa attcaatatc tccgccgaca ggaagaccat gggccaatcg cgttactttg
     4741 attccctggc ggtgtaaaga tacagccagg taacgagaaa gaacctctcc atccatatcc
     4801 ggattaaaag ccaatatcac ttcattaatt ttttcccggg cgagcaattg ctgcagcggc
     4861 tgaagattta tgtcccccag atcgatatcg tccatcaggt taaacccccg atttaacgca
     4921 taatactgtc cccgataacc acttctttcc agcgcgatga tatcactgct gtctgccacc
     4981 acgcaaagca aactacggtc acggctcgtg tcacggcaaa tcaaacaagg attttcatcg
     5041 gttaaaaatc cgcaatgcgg gcagggaaat actttttccc gagcaacaat gatcgattga
     5101 gcaagttgtc gggcttcatc aaccggcatc ttcagtataa aaagagccag tcgctgggcc
     5161 gtcttggggc cgatgcccgg cagcttctgc aatcgatcaa taaggttttc caacggccgg
     5221 gccagtctca tactttcaac tcctcggcaa cttaaaatcc gggaatattg agtccgccag
     5281 taattttgga catttcaccg gataccatgt cctgggactg cttgatagca tcattgatta
     5341 ctgccagaac cagatcttcc agcatttcaa cgtcttccgg gtcaaccact tccggcttga
     5401 ttttaatttc cagcaattcc tgccgtccat tgactttggc tgtcaccgcc ccgccaccag
     5461 cggaagcttc tacaaccttt tcctgcagtt ccgattgcat tttagccatt tgttcctgca
     5521 ttttcttggc ctgcttgatc atttgattca tattggcccc accgccagga ttaaatttca
     5581 taacgaatat cctcccttaa tcttttatct ctatattttc cttgccaaaa tactcaattg
     5641 ctttttgtac aacgatatca ttggtttgtt tttcatccaa aaacaaaaac tggatttcca
     5701 tttcccggtt aaatatttct ttgatgactt gatccaggat ttcccggttg gctttctcct
     5761 ccattctctc tttgtggaat cggtaacctt tggtataacc aatatacatg gtatcccctt
     5821 tggtacctaa caatttgccc tgcgccagca aagcgtgggt tggcaccttc ttttccttga
     5881 cacccaacaa aatacggttc caaaggatct cccgagcatc atttttaacc ggcttgtctt
     5941 tgcttgtggt cgtatctttt ttccttggtt caggagatgc ttccggttta ctggtttgcc
     6001 ctgaaataac cgcaatcatt tccaggaagg taatttccag cagaaatctt tgtccttcac
     6061 taaacctgag tttctctgcc aaatccattg tcatccgtaa caattgaaga atctggtctt
     6121 tgttcgctag ttgggtttgc tggatgagac ggtctcgatt ggcttcgctg accattagca
     6181 attcagcttt gtcgccaatt agtttagcca tcaaaagatc ccgcaaatat aaggaacatt
     6241 ccctcaccag ttgggtactt tcctttccct gctggacgga aatatgaatt ttgtgaacta
     6301 atgttcctgg atccttgccc agcactgcat cgaatatatc tgccacaaaa ttgtcatcga
     6361 ccaggcccaa tacctccagt acgttctctt tgctgatatc tttttctttg aacgaataaa
     6421 cctggtccag cattcccaat gcatcacgca gcccaccgtt ggcccgacgg gcgatcaaag
     6481 ccagtgcttc ttcctccatt acaattccaa aacgatttgc cgcgtccctt aacctggcaa
     6541 taatctggtc tgactgcaaa cgtttaaaat taaagcgctg gcaacgtgag ataatggtgc
     6601 tgggaatttt ctgcggttca gttgtagcca ggataaatat aaccgagtca ggaggttctt
     6661 ccaacgtctt cagcaacgca ttgaatgctt ctgccgtcag catatgaact tcatcgatga
     6721 tgtaaacctt tttcttgcct tgtgccggca tcacccgtac tctttcccgc aggtcgcgaa
     6781 tctcgtcaat accacgattg gaggccgcgt caatttctat gacatccata aaattaccgt
     6841 tattgatatc cgtacaagcc gagcactgat tgcacggttc tgcatccacg ggatgctcac
     6901 aattgacagc cttggcaaca attttagcga tgctggtttt tcctgttcca cggggaccag
     6961 aaaagatata ggcatgggta aggcggtctt cccgaatcgc attctgcaat gcgataacgg
     7021 tctgttcctg accaactact tcgataaacg tttgtggccg ccaaactcta tacaatgcca
     7081 gataagccat actcatgcct ccttcgtttc tattatttta tcttattcaa tccacttgta
     7141 atagacaaat acgaaaaaaa gcaggggttg ccctgccgtt ctattataaa ttgccgtgca
     7201 cctggcttcg aaactacctt ccggacgtta cttgaacagt taactccatt caggcgaccc
     7261 ttcgtcacac gaaatgtacc tcttagtgct gcttccttcc ggacctgaca cggttcaagg
     7321 ggttccgttg cgaaggaccc aaacttctcc gtcacttatt caagccagac cccacaagat
     7381 aagcccctcg gccaggaatt cgatcctgct gtagcgggtt gcaggttaca gggcaccgct
     7441 aattccccat ctagcacggc aaatctgtat tcacttggca gccggccaaa ggccggtgcc
     7501 tgtcttttgc ttgtctattg tacattcttt tacgccgatt tgcaagttgc agcaacattc
     7561 accccagagc catttattaa caaaatcaaa ataaaaaagc agaggtttag cgtttaactc
     7621 tctgcttttt acggacattt ttatattaat ttattttgat ttaggtgtca tgcgtgttcc
     7681 aatatccttt atttcattac tgaaactggt gagcggtctg ccggcggcga ttccctgtgc
     7741 cactctcttg attctggtaa ccgtatcaat atctgaactc accataatct tatatgaagg
     7801 atccatgttt ttaacattat taagcactga gttttctgtc atgtcagcct tatcctggcc
     7861 aagatcggct tccagatcca atccgatgta cattgatttg cctgctacga gagcagttgc
     7921 gtcgcggacc ccatctgctt tttctgcttc cttttctgct ttcatagcaa tctgcctggt
     7981 ctcagcagtg gttgaagtca tgctattgtc cggtgaggtc atgtagggtt tctgggttgt
     8041 cggtggattc ggttgcggcg ttgtcggttt ttgtgaagtt tgacatcccg ccagcagcaa
     8101 actgaatccg attgcacaaa ccatcattgt caataaaagt ttttttgtta gattcaagaa
     8161 cgacacctcc tgtttgtttg ttttattact atcattacgc tactattatt tgttgctgcc
     8221 tatgatccta ttcgctgtaa taaaaccaag cgatgccttt ttatttagaa ttaatttttc
     8281 acagcaaaag agctgatctt tgatgtaaat tgcataatga tcagctcttc tgctccgggt
     8341 tggtactgaa caacagtcca ccctcctttg tatatcatta tcctggcaac cgggctacca
     8401 tagcttgcac cttaggcccc tggctttgtg ccccctcctt tcgaagagtt tacctccaat
     8461 ccagtttccc aaacgaatta tatcaggcga aataatttcg tttgaaccgc ttgatcgaaa
     8521 acccttgtcc gatgccgctt atctgttcct cctccatgta tcttgacagg agccttgcac
     8581 tctgctcaga accatggatc taatctgcct gcccgcagga tctggccaga ttattaacaa
     8641 tgattttggt ccagacaata ttttctgaca agcactggca cccaggataa tttttgaaca
     8701 gtactttagc tgatgagcta tttcctgtta tattaatttt attaaacgat agataccctg
     8761 tcaatgtttt gtctgaatat tttgactatt ttgttgcttt ttcttcccaa cgtgattatt
     8821 ttgcagaatg aaaaccatat cataataatg cttgtgatga aaaaggagtt gcccgatcat
     8881 catggaatgt caatcttttg cccccatctt gcgagaagac gcccgggtat tgcacaaaaa
     8941 cttgccgcgt ggatgattat taaagattat ctttgattgc aaataaataa cgattgaaaa
     9001 cagaaaacca ggaagccttc cttatatata aggaagggct cctggcttga acataaaacc
     9061 tgctgatatt atatatgatt cgggagatca catataaatc caaaaccagt aattattaaa
     9121 tcaacgtgta acaaactgct taattcatct tggcggcata ttcagcttta aaatgtttca
     9181 gcgtggtaag aaccggtgcc ggagccgcct gacccagacc acacagacag gccacacaca
     9241 taaccccacc caggttttcc atcaaatcca tatcggcaac tgtgcctttc cctgcattga
     9301 tcttgtgcat gatttcatgc atgcgtttgg ttccttcacg acaaggattg catttgccgc
     9361 aggattcatg ctcgaagaat ttgctgatac gatcaacgat atcaacaata tcacgtgttt
     9421 catccatgac aaacacagct cctgttccca gggttgcccc gattttggcc atggaatcaa
     9481 aatcgatggg ggtgtcgagg agctgttcgg gaataaatcc gccggaagtt ccgccaatct
     9541 gtaccgcctt gaaattcttg ccgccaacga tgcctccacc caaaccaaag atgacgtcgc
     9601 gaatggtgac atcagttggt acttcaaaat aggtccggtt gaccacatcg ccggtcaggg
     9661 taagaatttt ggttcccgga taggaagcgg cgccgatgtt cttaaaccaa tccgcacctt
     9721 tctccacaat cgccggaata tttgcatagg tctcaacgtt gttgacaata gtgggtttcc
     9781 cccacaatcc ctccactcca gggaatggcg gtttaaatct gggttccccc cggtgtcctt
     9841 cgatggattc gatcaatgca ctttcttcac cgcatacata ggctccgccg cccattctta
     9901 cctcaatgtc aaagtcgcct aaaacacctt tgactttcgc ctgggcgatt gcctggttca
     9961 accgttccac cacataggga tattcccccc gacaataaat gtaccctttg ttggatccaa
    10021 tagcatagcc gcaaatggcc attccttcga acaggctctg gggatcttcc atcatgataa
    10081 tgcgatcttt ataggtcccg ggttcgcctt cgtctgcgtt gcaaaccaca tatttctgat
    10141 ccgtttccag catgtatgaa aaactccact tcatgccgca attaaacccg gctccgcctc
    10201 tgccgcgcaa ccctgaattt ttcacttcat caatcagggc tttgcggtcc atgcttttag
    10261 ccttggcgag tgacttgtat ccgcccgcgt taatataatc atcaattttc aagggatcga
    10321 ttttgcctac attacgtagt acgatgcgca tttcttcagc cacggttttc cctcctttcc
    10381 cctcttactt gtactctgcc aaaatggcag ggattttctc ggcagtaaca tcgccataag
    10441 gcttgctgtt aatggtaatc accggcgtag cctggcattg gcccacacac tcggtgaatt
    10501 caagggtaaa tttgccgtcg gaagttgatt cacccatttt gatacccaat tctttttcca
    10561 gcgcggccac cactttgtct gccccagcaa tatgacaggg ggcgctttca cagatgcgga
    10621 tcacgttttt accgcgcttg ccgactctca aataagagta aaaagtagct actccatgtg
    10681 cccggggttt ggaaacacca aatacctggg cagcataggg aatcgcctct tcaggtatat
    10741 aactaaattc acgctgcaaa gcatgatagg cttcgatgat tcctccgggc aaatctttat
    10801 aggcatcgat gatactcttg tagtctgcca ttattgattc acctcctgga gtttttcaat
    10861 ttttactgca gcgactttat attcgccggt cccggtaatg ggatcaaggt ggtgactggt
    10921 cagcgcattg gtgggtgtag cgttgtaatg gaacgacatc cagatgacac ctggcaatac
    10981 ccggtcagta accttgacat gcgtatcggc aacaccgcgg cgggaggtaa ccctcacttt
    11041 gctgccggtt acaagcccca gtttttctgc atcaaccgga ttgacctcgg ccatttcctt
    11101 gtcccaaaca ctgctaatcc ctttggaata aggtgtggtt acattataat ggtatagaat
    11161 tcttccggtt gacagcagga agggatattc ctcatccggc atttctcctg gaggtatatg
    11221 ttctgagggc tggaacagtc ccaagccgcg agtgaatttg ccggcatgca ggaaaggcgt
    11281 gcctggatgg tcaacactcg gacagggcca ctgcaggctc ttcagttctt ccaggcgatc
    11341 atagttgata ccgttcatgg agggtgaaag ggacgccaat tcatcccaga tttcactcgg
    11401 atgattgtag ctcatgggat aacctgcccg ggttgacatc tcacaaatag tctgccagtt
    11461 agcctgaccg ggaataggct caatggcttt acgaactctt tgcacccgac gttcggtgtt
    11521 ggtaaatgtt ccgtcacact cggcaaaact agccgccggc aggattacat ccgcatattc
    11581 agccgtttcg gtcaggaaca attcctggac aactaaaaat tccagatgct ccagggctcc
    11641 acggatatga ttagcgtccg gatcagtcaa taccgggttt tcgcccaaga tatacatggc
    11701 ttttacttta ccttcatggg caccagcaaa catttccgga atcttcaagc caaccttgtt
    11761 ggataatttg gcattccaag ctttttcaaa tttctcatga actgcatcta ccgtcacatt
    11821 ctgataacct gaatatacat tgggcagtgc gcccatgtcg caagcaccct gaacgttgtt
    11881 ctgaccgcgc atcgggcata caccggtacc gggacgtccc agatgcccgg tcagcatggc
    11941 caagttggca cagctcatta cgtttctggt tccgcagata tgctcggtaa ttcccaaggt
    12001 atagaatatc atggctttgg aagtcgtagc ataaagacgg gcaaccgcat agagatcatc
    12061 aaccgatacc ccggtcagtt ctgatactct ctccggcgta tattcggcta cggtagcctt
    12121 gagttcttcg taattttcgg tacgttcctc aataaacttc ttgtcttcca ggccctcctt
    12181 gatgataata tgcattaatc cattgagaag cggtatatcc gttccaggtt tttgccgcag
    12241 ccagtaatca gcttcgtcaa ccagatcaat atgacgcgga tcacaaacca ccattttggc
    12301 tccattacgg gcggcttgtc tcattttata accaataacc ggatgtgctt ctgttgcatt
    12361 ggtaccgatc aggaacatca actctgcttc attggtaatg tcactgattg gattggtcat
    12421 cgctccactg ccaaaagttg tagccagacc ggctactgta ggagcgtgtc aggtacgggc
    12481 gcaatggtca acgttgttgt tgcccatata cgcgcgggta aattttgata ccagataatt
    12541 ttcttcattg gtacaacgtg ctgaactcaa agctgcaaag gattcggacc catatttgtt
    12601 tttgatttca ttgaaacgtt tggcaatcaa gtcataggct tcatcccagg aagcctcttc
    12661 aaatttgcca ttcttcttga tcaaaggact ggtgagacgt ttttcattgt aaatgaaatc
    12721 ccagccaaaa cgtcctttta cgcatagact gcgtccatta acagtagcgt cgggactgga
    12781 caaaaccccg atgaccttct tgtcttttac cgccagatcg aaagtgcatc ccgtcccgca
    12841 gaatggacag gtcgtccgaa ctttttcgat ttcccaacgc cgggcttgcc cggccatcgt
    12901 tttttccgtc aaggcaccag ttgggcaaac tgctacacac tgcccgcaaa atacacaatc
    12961 ggaattcata tagtctacgt caccgtaagt ggttacttta cggtgaaatc cccggtgcag
    13021 ataggagagg ttgtcctgtc cggtcaattc ttcgcaagcc ctcacacaag ctccgcactg
    13081 aatgcatttg ttcatatcgc gattgataaa ggggttgctg tcgtcaatcg cataatgatg
    13141 ttgttccccg gaaaagggac tttgcttcac gccgtattgg taacagtagt cagccagttt
    13201 gcaatcacct aatttgtcac aggtcagaca gtcgagggga tggttggcta tcaacaattc
    13261 caaaatagtg cgccttgcat tgatgacagc cggagattcg gtctgtacct ccattcctgg
    13321 ggctacgggg gtcacacacg acgctggcag caaccgattg cctttgactt ctactacaca
    13381 catccgacat gaacccatgg ggctcaaatc cttgaggtag caaagtgtag gaatctcaat
    13441 gccgttcgat tctgcagcct gcaaaatcgt actgcctcgc ggaacttcga cctccttccc
    13501 gttaatcgtc agtttcacta attccacttc catcactccc taaaattttt ctacaaaaac
    13561 acctccagca ccatggctgg aggtgtctat atatactcca tcagggatag atagtcccca
    13621 acaagcatca ttacggtcta ttcagaccat tactcctttc caaccacggg aggcaaaccc
    13681 gtttccagac ttaccctaat ggccattcta ccctagtaaa accttaggca ataatggctc
    13741 ggagatttct attaaatttg gttacctgta caaaaattca cgaactccct tgccttgttt
    13801 attctgtata aattgatata atcctgcaca gattttttaa aaaacaaaaa tatttggatt
    13861 atataaccca tatcaatgtt tcgcattcaa ttcgtttata aacgcacttt cccggttcaa
    13921 tcggctcaat cgctgtatat ttatattttc accgattcaa gaacaataag cactacttcc
    13981 tgtcctgcat caaccggtgg actgccggcc ggcaagtcaa taagagcgtt gcattgcagc
    14041 aaagatctaa tcatgcttgg cttctgtcca ggcagtaccg taatctgcca tcctttttcc
    14101 gtccaagtca gctgtccgcg aacaaaacgc cgtgtgccgg cttttttggg gaagccattg
    14161 ctgcatatcg ctgaaattct tttataaccc ggatcatatc cttgcatgcc ccgaagtgcc
    14221 ggggcaacaa aaagatgata ccccaccgag caggcagcgg gattgcctga caacgcaaaa
    14281 atctgtttag agtgtttaag ggcaaaacca atatggcttc cgggttggac cgacacgtcc
    14341 caaaatatta tttctgcgcc cagttttccc atcaaggaag cggcttcact ttctccatca
    14401 gcataggttc ctccggtaat gatcaaaatg tcacactgcc ccaacagcct ttcagccgcc
    14461 tcctgaagcg aatccatgat gttgtcggag gtacagcccc ccaccgggat tccgccatcc
    14521 cgaatcacca aggagctcag catgggacta ttgctgtctc tcatttggcc tggttgagga
    14581 tttttttgcc attcaacaac gttttcgcta agacacagca ccgccactct gggccggcga
    14641 tagacctcaa ttgctgccat gccaaaggca gccagcaacc ctatctcgcc tgcccccacg
    14701 atcgagcctt ggcttgccat taagctatcg gcagggaaat cttcgccggc ttgcttgata
    14761 ttgttgcccg gcttgatttc ttcccgggga tatagacttt ggcctgatat ttctgttttc
    14821 tcatgaggga tcactgccgt cgttccaccg ggtagattgc ctccggtgca aaccgccaag
    14881 gcctgtcctg gcagaagtaa ctccgtcgga atctctcctg gctttccttt tccgatcaag
    14941 cgatattcag tcctgactgc acataaagca tagccatcga cagccgactg ttgccgaaca
    15001 ggcaaatccg ccggagcatg gagatcaact gcactaaccc tgcctagggc cgtcatgatt
    15061 ggtatgcttt ccgcatccag cggctggcag tattgtaaaa tttgattgta cgcttcttcc
    15121 aggctaatgt taaccagcac cagcaatttt cctccttctt taaaaaattg aagctttgcc
    15181 gaagggacac tgcggaaaat gacattgttt gcattcctcg cacaagccgc catgacccat
    15241 ggctacaata tcagaacgtt cgattttatc ttcggcaaaa atacggggca gcaaaagatc
    15301 caatgttgtt ttgcggctga aaagagcgcc accaggcact ccacatatcg gcactcgccc
    15361 ctgataagcc agcataaatt gcgaacccgg aaggattggg gcgccataga ataccacttc
    15421 agcccctgat tttcgaatcg cctgcggcgt cgcatcatct gcatccaccg acattcctcc
    15481 agtcacaatc accaattggg ctccttcggc cacaaaatta tgaacctccc ggctgatgag
    15541 ttccggatca tccggaacta ttacctgtcc catccaccgg ccggaataag gagctatttt
    15601 gttgcgaata acaccggcaa aaccgtcctt gatacgtccg ctgttgactt cggtccccgt
    15661 ggtgacaacg gctgtccata aaggcttgaa tggtttgact gacaaaatcg gattcttgcc
    15721 agcgcaaata tttttggctt cctgcaaaag tttttgctcc actgccacag gcacaactct
    15781 ggttccggca actgtttggc cttctactac gacccgatca ttatgcaatg tcgcaaagat
    15841 gacatcctcc aggttgttaa gcatgtgaag agcatctgca ttaactttca aaagccctgc
    15901 atattcagct ttaagattga cccggccctg attgggctct ccccaatata gacctgcacc
    15961 agcagcacat tgagccagat gtagtgccgc gtcatcttca tggaccaagt catcatcagg
    16021 atcccagata tagatgtgtt ccttgccaat atcctttaat ttggaaatgt cttccgggga
    16081 aatgacctgg cctcggcgaa aagctctgta tttttctcgt cccggaacaa ttctggtaat
    16141 atcataaccc aaggtcaatc caaccgcttc atcgactggt acttttcgca tataaccctc
    16201 ctagctttgc atgcctgttt gttattgcca tgaatagttc tattctatta tgaatctcct
    16261 ataaatgaaa cacctccagc atagtcgcca gaggtagctc attttatgaa aggaaccttt
    16321 gtttatcttt tttcagcttt tccgagtaat catttatctt ttggacggtt taattctcca
    16381 cgaaaaatac tcttcccgga tccataaaaa cgtaaaaagg tgattgtaat ttgctgatct
    16441 tttctctttg ttcccggctg atttcccact gaatatgata atcatccgtt ccattggcag
    16501 cggaaccgat tttgagatat aacgtagtgc gaaacggcga ccggctttca tctgcaatcc
    16561 aaaccgggaa acaattattc ccagcactct ccgcaaacat aatatggttg gcccttattc
    16621 caacaaaacc gtttgggctg agaatactcg tgtcagtatg caacgttatc ccccaagcag
    16681 gtacttctgc tgtataagtt gatttgcact gggcggcagc aatgttttta caccccgtaa
    16741 tctgagccgt ttccaacgat actggatgca aaaacacttc ttccttggaa gcaaaagtct
    16801 ctacctgacc cgcgtttaat accgcgatat ggtcacagag acgatagctc tcttccatat
    16861 tatgggttac aaacaaggca tttcctttaa attccttgag ggtgagctgc atatccttca
    16921 ttagatggtt ttttaaatga ttgtccaggg cggaaaaagg ttcatcaagc agcaatacct
    16981 ctggttctac tgccagcgcc cgggccagtg cgacccgctg ttgctgcccg ccggagattt
    17041 gggaaggata acgtttggcc atatctgtaa tgtttagccg tttcattaat gcaaaggttt
    17101 tttgttgttg gtctgttttg gggaacttat ccaaaccaaa tgcaatattt tcttcaatcg
    17161 ttaaatgagg aaacaaggcg aaattttgaa acaggaatcc ggtcttcctg taacgtgcag
    17221 gtaaattaat tttttgggca gcatcaaaat aagtcttgcc atttacgatg atctggcctt
    17281 catcaggacg aaccaatccg gcaatacaat gtaacaacat actttttcct gcaccggaag
    17341 cgcccaatac accaataatt tcctgctcac tagaaagttt tatctgtaat gtaaatccgg
    17401 agagtttctt ctttatgtct atatataacc ccattttatt ttcctccggc acccagcttt
    17461 aattttctct gggcattatt ccagttattc atgattacaa tcaccgtcag cgaaatggag
    17521 agaatgacca gtacccagaa aagagcaact cgcatctctc cgcccaaggt ggcaaaataa
    17581 atcgcaatcg ggatggtttg ggtttgaccc ggaatactgc ctgctaccat taaggttgcc
    17641 ccaaactcac ccatggatcg agcaaaagcc agggctgttg cggcggcaat accgggtcgg
    17701 gccagcggta cggatacacg ccagaagact ttccattctg aaacccccag agttctggcg
    17761 gcattgagga tgttgggatc aatctgttca aaggcccccc gggcggtttt atacatcagc
    17821 gggaaagcca ccacaattga ggcgatgact gccgcatgcc aagtgaagat gatattaata
    17881 tcaaacattc ccaacaattt gccgatgggt ccattcttgc caatcaacaa caagatcccg
    17941 aatcctgcta ccgtaggggg cagaaccaga ggcagtgtca ggatgccgtc caacagcccc
    18001 tgccacctgc cccgatagtt taccatccac cacgccgcgg ccagtcccag aaaaaagatg
    18061 atgatggttg cgatgagcgt gattttaatt gaaatgaacg ccggggacca atcaaaatgc
    18121 atggcgatta ctcctaaagc ctggatcatt ctataatact tcaaacccat acttgtcaaa
    18181 tactgctttg gctttatcgg aagtcaggaa attcaagaaa tctctcgaag ccgtatagtt
    18241 attggtggac ttgattaacg cggccggata aacaatgggt gtatgagatt cttccgaggc
    18301 ggtcgcaatg actttaacgg cagcagaaat ttttgcgtcg gttaaatata caacccctgc
    18361 atcaacatta ccggtctcaa cccaatttaa tacttgtctg acatcctggg catagacaac
    18421 tttttctttt accttatcga gaatatttaa gaaggtaaat acattttcag cgtattttcc
    18481 ggccggcacc gttttcggtt cccctaaagc cactttctta acggaaggat ccgttactac
    18541 cactgaaaaa ctgctgttga caggaaccgt tgaatcattg ggaaccacca gaaccaactt
    18601 gttgcccagc acattacgga tcgtactgtc aatcaaaaga tctttggttt tcaaaacatc
    18661 catattggaa gcagctgccg agaaaaataa atcaacatct gccccttgtt cgatttgttt
    18721 ttgtaaggta cctgatccgg caaagttaat cgtcaaggtt gttttgggtt tttcttgcag
    18781 atataaagac tgtacgtcgg ttaatgcatc ttttaagctg gcagcagcag aaatagtcaa
    18841 atctaccttg gtggtgatat tcgctgtcac aaccccattt tgattggcag ctgaaacccc
    18901 gtaacccatg gcattggcaa ctgatctgac cggaatataa agacgcccgt tcttgataaa
    18961 tgctgttgtg tccatgccaa tcgtgccgta ggaagtggtt atttcatttg aacccacttt
    19021 gatctgaatc gttccgttaa tggttgccgt attgctgccc ccatcccag
//
LOCUS       DAOTHZ010000040        18894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3513_length_18894_cov_7.426456, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000040 DAOTHZ010000000
VERSION     DAOTHZ010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 18894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18894
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_18894_cov_7.426456"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="P5549_06185"
     CDS             complement(<1..178)
                     /locus_tag="P5549_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12696.1"
                     /translation="MPDIKSQIDELREEIRQHEYQYYVLDDPLVTDAEYDALLRELIR
                     LEEQHPELIGPDSPT"
     gene            complement(716..2803)
                     /locus_tag="P5549_06190"
     CDS             complement(716..2803)
                     /locus_tag="P5549_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRW12697.1"
                     /translation="MKHNFEGLNEAQIEAILHRDGPCMVLAGAGSGKTRVLTERINYL
                     IENGVRPDAILAITFTNKAAQEMRSRIRDMRPDYSGKWIQTFHAACYKILRMDIHLLG
                     YERNFAIIDDSEGKTLLKEILKEERDYETKPEEVLYSIKQAKNSLMDAEPYFHNLNLP
                     LHIKDKYYRVFRIYNARLREYNALDFEDLIILTIKLLREYPDILTKYQNWFQYIMIDE
                     YQDTNYAQYLWANLLAAAHHNIFVVGDPDQSIYSWRGAEPYNVKKFLKDYPRVHLIKL
                     EKNYRSTGSILKAANAVIKNNRDREEKELYTDKPEGEGLIYFCAGDSFQEAQFVADTI
                     ADLVDRDHKRYQDCAVFYRTHAQSRILEEALLRRYIPYQIVGARKFYERKEIKDILAY
                     LKLTCNPNDHLSLRRIINVPRRGIGEKTLEKIEEFAQHEGMPIMEVLADISRVPGISR
                     KMSTSLEEFYGMIQYFVSLKEAGVALKEILDQVMDMSGYVEELRKNNAADAQTRIENL
                     QELRSLAVEFSKEGIEDLDEFLASIALVQDTDDVNNADTVMMMTYHGAKGLEFPIVFM
                     TGMEEGVFPSYRTETEEDMEEERRLCYVGITRACERLYLTNAISRLLYGYERNNPPSR
                     FIKEIPAQLLTSPHVKKAAACVLAEGDTVIHRKFGMGVVLQLQEGQIAVVDFERAGVR
                     TLRLDIAPLEKIS"
     gene            complement(3074..3328)
                     /locus_tag="P5549_06195"
     CDS             complement(3074..3328)
                     /locus_tag="P5549_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12698.1"
                     /translation="MPWPGNTNKPTRSITRAIPNEKDYASCLDYTINSFTIDQEALYA
                     AMQEHGDNEITLTATLQFPERSTTADTATWTKTIVVTLAD"
     gene            complement(3331..3981)
                     /gene="hisIE"
                     /locus_tag="P5549_06200"
     CDS             complement(3331..3981)
                     /gene="hisIE"
                     /locus_tag="P5549_06200"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRW12699.1"
                     /translation="MKDTIKFDAHGLVPAIIQDAQSGQVLMLAYMNEESLDKTLASGR
                     TWFYSRSRQKLWMKGESSGNVQKVKQILYDCDADTLLIKVEQTGVACHTGHYSCFYRD
                     STGQEVENQLFDPKEIYSSASNRGILIELYNLIRERKEQLPEGSYTSYLFSKGIDKIL
                     KKVGEENAEVIIAAKNDSKSELIYETSDLVYHLLVLLVERGIGPDDIFNELALRRR"
     gene            complement(3978..4763)
                     /gene="hisF"
                     /locus_tag="P5549_06205"
     CDS             complement(3978..4763)
                     /gene="hisF"
                     /locus_tag="P5549_06205"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRW12700.1"
                     /translation="MLAKRIIPCLDVHAGRVVKGINFVNLRDAGDPVELAAYYDREGA
                     DELVFLDITASSDQRQTMIDVVRNTAREVFIPFAVGGGIRTLDDIRAILNAGADKVSI
                     NSAAVHDPTLITEAARRFGRQCIVLAIDARCRKADVSEAQGTGRKWEVYINGGRTPTG
                     LDVLEWARRAEKLGAGEILLTSMDKDGTKDGYDLELTAAVADRLPIPVIASGGAGQLE
                     HLREACVEGKADAVLCASIFHYREYTIAQAKEYLASKGVAVRL"
     gene            complement(4763..5497)
                     /gene="hisA"
                     /locus_tag="P5549_06210"
     CDS             complement(4763..5497)
                     /gene="hisA"
                     /locus_tag="P5549_06210"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRW12701.1"
                     /translation="MIIYPAIDLKEGQCVRLVQGKAEKKTVYSSDPAAMAASFQEQGA
                     QWLHVVDLDGAFQGRCGNHDVIQKIAAQITIPFQVGGGLRQLEDVENVLAMGARRVII
                     GTRAVTSPAFVEDLLYRFGAEKIILGLDARDGMVAIEGWVEKSSLPVLDFARQMRNLG
                     VKTAIYTDVSRDGLLQGPNLAAIQQMAEQSGLKIIASGGVSSLENIKSLKKMEPLGVN
                     GAIIGKALYDGKIALKNAIQAAKGEE"
     gene            complement(5497..6105)
                     /gene="hisH"
                     /locus_tag="P5549_06215"
     CDS             complement(5497..6105)
                     /gene="hisH"
                     /locus_tag="P5549_06215"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRW12702.1"
                     /translation="MIAIIDYGMGNLASVSNAFAKIGYDNIITRDPEQILAADRVVLP
                     GVGAFADAIGNIRERGIDQAIHELVSRNTPLLGICLGYQLLFSESDEDGLHKGLDVIA
                     GRVEKLQIPASFKVPHMGWNNIEIKPGSQLFAGIPQGSWFYFVHSYHTVPEDDTVMAA
                     AANYGIKFVCAVEKGNLVATQFHPEKSSDMGLQVLRNFGAMQ"
     gene            complement(6140..6730)
                     /gene="hisB"
                     /locus_tag="P5549_06220"
     CDS             complement(6140..6730)
                     /gene="hisB"
                     /locus_tag="P5549_06220"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRW12703.1"
                     /translation="MDVRSSKIVRQTAETQISLSFTVGGSGKHQIETPVPFLNHMLTL
                     FAVHSRCDLIVNATGDTQVDDHHTVEDIGICLGQAIKEALGEKKGINRYGQALIPMDE
                     ALARVVMDLSGRSFMQYAVEIPSPQLGNLSTENVKEFFQAVANQAGMTIHIDLLRGDN
                     SHHIVEAVFKAFARAFKEAVTVIAGVEGAWSSKGQL"
     gene            complement(6733..8016)
                     /gene="hisD"
                     /locus_tag="P5549_06225"
     CDS             complement(6733..8016)
                     /gene="hisD"
                     /locus_tag="P5549_06225"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRW12704.1"
                     /translation="MKIRRLQTKEEILAYLGSHQEDMQQYEQRVQEIAQAVKARGDEA
                     IFEFNRRFDGAEISHDNFLVREEEIDEAYELVDDDYVFSLRKAIDNIDYFHGKQLRNS
                     WMEPDERGNVLGQLFRPLERVGIYVPGGTAAYPSSVLMNAIPALVAGVQEIAMVSPPD
                     ANGKMNPYTLVAAAELGLTEIYKMGGAQAVFALAWGTETIKKVDLISGPGNIYVTLAK
                     KMVYGDVNIDMLAGPSEILIVADRFAEPAYLAADMMSQAEHDVLARSILVTDSEPLLD
                     AVEKELNQQLAGLSRYAIIEQSLSNYGAMILVDDINEACRVANLVAPEHLELMVEKPF
                     EMLSKIHNAGAIFMGSHTPEPVGDYLAGPNHILPTGGTARFYSPVTVDTFMKASSIIY
                     YSREGLEEDADDIIKLAEVEGLTAHANCLRVRKDK"
     gene            complement(8013..8669)
                     /gene="hisG"
                     /locus_tag="P5549_06230"
     CDS             complement(8013..8669)
                     /gene="hisG"
                     /locus_tag="P5549_06230"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRW12705.1"
                     /translation="MDNILTIALSKGTLLKPTIELLQQAGLPCEGISEDSRSMIFTFS
                     GDPQIKYIICRPTDVPAYVEHGAADLGIVGKDVIVEQGKDLFEMADLGFGYCRFVVAV
                     PAHMKHLTLQELNYKRVATKFPAVAEKFFRDNGLQVEIITLHGNIELAPLMGLSDMIV
                     DIVSSGRTLRENNLVELVSIMDSTTRLVANRVSYRTKYQEVQPLIEKMQMIVKGGKNQ
                     "
     gene            complement(8681..9847)
                     /gene="hisZ"
                     /locus_tag="P5549_06235"
     CDS             complement(8681..9847)
                     /gene="hisZ"
                     /locus_tag="P5549_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRW12706.1"
                     /translation="MDNRQIPKGLRDLLPQEVKNRRKLEQKAMQIFTSYGYKEVMTPT
                     FEFLEVVETGAGKHRERLFLFMDREGGILSLRPEMTVSIARLAAMHLRDSIYPQRLFY
                     SANVFRHVQPHLAQYREFWQTGIELLGASGPWADAEAISLAVKLLQELGLNDFKISIN
                     QIGIFNGLLTAAAIDSEDKDEIRRLVEKKDLVELERRLENLVMPDAWKESIARLPVLH
                     GGLEVLQKIPYLEQHAATAQAVEDLLRVYEALQDYGVTRHIVIDMGVLRELELDYYTG
                     VIFEGYSPELGYGLLGGGRYDNLLGQFGFPCPATGFALGMDRLALVLARQEEETNRYL
                     LGGRNLRALVEKAQQLRGEGKTVEMDVEGNSLEALQEKVRGRGDISLLYLDEQS"
     gene            complement(9840..10127)
                     /locus_tag="P5549_06240"
     CDS             complement(9840..10127)
                     /locus_tag="P5549_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRW12707.1"
                     /translation="MDENAKLLVQALLCLENEEEACRFLDDLCTIAEIKAMAQRMHVA
                     RLLDNDTTYSVIARESGASTATISRVKRCLNYGARGYRLVLDKLNKELNHG"
     gene            complement(10198..10860)
                     /locus_tag="P5549_06245"
     CDS             complement(10198..10860)
                     /locus_tag="P5549_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRW12708.1"
                     /translation="MVTLEMVKANQKVKDFMDMGNTFLGNIGAIEHDLHHAQLTSQLA
                     RDILQALGYPDRIAELAAIAGYLHDIGNLVNRYGHGMSGALIAFQVLNEMGMETEEIA
                     IIMGAIGNHEEHAKGCAVNHVAAALTLADKSDVNRSRVRKQDFATFTARDRVNYAVTD
                     SRVIVQKEEQIITMQLQIDTSVCSVMEFFEIFLTKMMMCQHSAEYLGCEFKLVINDTR
                     LL"
     gene            complement(10909..11442)
                     /locus_tag="P5549_06250"
     CDS             complement(10909..11442)
                     /locus_tag="P5549_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12709.1"
                     /translation="MTVNKAPYVRMFIFLVVVVMLFFASTREFLKITFFLGIPFLLFY
                     VLMSRQIRYSLWWFTCGILVLGVIGLYGYMLVQLPQRVEVREIIREGGTLVAEGKYDQ
                     AIEAYQRLEELGQTEKMEEKINLARQEQQAAWQLEEARQKLVQGDQEAARQIIANIEP
                     GTRAAVEARDLRKRLEL"
     gene            complement(11514..12797)
                     /gene="purD"
                     /locus_tag="P5549_06255"
     CDS             complement(11514..12797)
                     /gene="purD"
                     /locus_tag="P5549_06255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRW12710.1"
                     /translation="MGKKLLVVGSGGREHALVWKLAQSPGVEKIYAAPGNPGISKLAE
                     CVEMAVDDIDALCQFALEKEIDLTVVGPEAPLTLGIADRFSAEGLLIFGPSAAAARLE
                     GSKVFSKNLFKKYKIPTARFEVFDDAEQARDYARILFDQGQKAVVKADGLAAGKGVVV
                     AECFAEASAAIDSIMNDKTFGAAGDKILVEECLQGEELSFFAISDGKDYLALLSAQDH
                     KRIYDHDEGPNTGGMGAYTNPPLYTGELKHQIITEVIEPTIRAMQAEGCPYQGVLYAG
                     LMITADGPKVLEYNARFGDPEAQVLMPMIKSDLLPVLEAAAQGQLPGRTVEFEAGACV
                     GVVLAAGGYPGAYQKGDAITGLDQLQEDILVFQAGTALKNDQLVTAGGRVMAVVACAD
                     SIPAAIAKVYSEVKKIHFKDMHYRHDIARKALRQA"
     gene            complement(12824..14341)
                     /gene="purH"
                     /locus_tag="P5549_06260"
     CDS             complement(12824..14341)
                     /gene="purH"
                     /locus_tag="P5549_06260"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRW12711.1"
                     /translation="MKRALISVSNKDGVVEFAQGLEKLGYEIISTGGTYKTLADAGVK
                     VMKVADITGFPEILDGRVKTLHPKIHGGILARRIPDHLQQLDANQIDPIDIVAVNLYP
                     FRETVSKPGVTVEEAIENIDIGGPSMVRSAAKNHADVIIVVKPEFYGPVLEELQQSGQ
                     VSREMRLKLALEAFTHTAAYDAMISAYLAKLTGNTFLDNMTVAGEKMYELRYGENPHQ
                     KACFYRTMTPGASLGDAKVLNGKELSYNNIIDVQAAWALVKEFEQNACVIIKHTNPCG
                     TAIADTLEEAYEKAFNADPVSAYGGIIAFNRQVDAATARKAAQTFMECIIAPGYDDDA
                     LAIFQAKKNLRVLEMEISSAPELQIKSVEGGFVVQESDQGSVTAADLQVVTEKAPTAA
                     EITDLLFAWKIVKHVKSNAIVTAKDGVSIGVGAGQMNRVGSAGIALTDTEKNKGAVLA
                     SDAYFPFSDTVELAASKGITAIIQPGGSIRDQESIDVCNQYCIAMVFTGMRHFKH"
     gene            complement(14374..15006)
                     /gene="purN"
                     /locus_tag="P5549_06265"
     CDS             complement(14374..15006)
                     /gene="purN"
                     /locus_tag="P5549_06265"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRW12712.1"
                     /translation="MKRIMEPGCLRLAILASGRGSNFLAILEAIKEGQLNACIEVLIS
                     DKEDALALQKAQEAEIPHVFIDPRLFSDRAGYESDIVKELQAREIDLVVLAGYMRLVG
                     KVLLHAYPHRILNIHPALLPAFTGLHAQRQAVAYGVKLSGCTVHLVDDGMDTGPIIAQ
                     AVVPVLPDDSEDDLAERILVEEHKLYARVLQWFAEGRVYIEGRKVLVNHN"
     gene            complement(15006..16043)
                     /gene="purM"
                     /locus_tag="P5549_06270"
     CDS             complement(15006..16043)
                     /gene="purM"
                     /locus_tag="P5549_06270"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRW12713.1"
                     /translation="MDKEGLSYKKAGVDIDAANDSVERIKKWVGRTRRPEVLADIGSF
                     GGFFALNQQKYLEPVLVSGTDGVGTKLRIAQMMDVHHTVGIDLVAMCVNDILVHGAEP
                     LFFLDYIAIGKLQPQLVEALVQGVSEGCVQAGCALIGGETAEMPGFYKEDEYDLAGFT
                     VGVVDKPKLINGEAMKDSDVLIGLPSSGIHSNGYSLVRKVLLESAGLKLDDYFDELGK
                     TLGEELITPTRIYVPSVLALLQEVEVKGMAHITGGGIAENLMRSIPAGLGAEIKAEAI
                     AIPPIFQMIARMGEVAPAEMFRTFNMGIGFILAVDPQQCDNALKCLRAQEEQPIIIGE
                     ITARQSKVVIA"
     gene            complement(16067..17482)
                     /gene="purF"
                     /locus_tag="P5549_06275"
     CDS             complement(16067..17482)
                     /gene="purF"
                     /locus_tag="P5549_06275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRW12714.1"
                     /translation="MLYELEDWASEGKWQDECGVFGIFMNNPVHQTDAARTTFYGLYA
                     LQHRGQESAGIAVANGQDIQLHKGMGLVSDVVKARHIEELEGNIAVGHVRYSTTGGSA
                     MVNAQPLVFHYLNGTVALAHNGNLTNAAELRQRLATYGSVFQTTTDTEVIANLLARYS
                     QDKLADALAKCMIDIKGAFALVIMTEDRLVGVRDPLGIRPLCLGSLNGDYVLSSESAA
                     LDTVGAEFIRDVQAGEIVVIEKDGMHSAQLGNANRLAHCIFEYIYFARPDSVLDGINV
                     NRARRELGKQLAWEVKIDADIVIAVPDSGTASALGFAEQSGLAFEEGLMKNRYVGRTF
                     IQPTQQMRELGVRLKLNAVPDVVGGKRVIMVDDSIVRGTTSKKIVQMLRTAGASEVHM
                     VVASPPTKFPCYYGIDTSRREELIANTMDNEQIRAFIGADSLHYISRAGLFKALQNEN
                     DSYCMACFSGEYPAGKALPEV"
     gene            complement(17554..18267)
                     /locus_tag="P5549_06280"
     CDS             complement(17554..18267)
                     /locus_tag="P5549_06280"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRW12715.1"
                     /translation="MQVREFLYEGKAKQIYATDDEKVVLIKYKDDATAFDGQKKGTIL
                     NKGIVNNRVSNLLFKLLEKHEIETHLLEQLDDRQTLVKKVQIIPVEVVVRNVIAGSLS
                     QRVGLEEGVELGEPIIELYYKNDELHDPMINQYHARVMGWATAEQLQEIDRQAQQINQ
                     VLKAFYDSIGIILVDFKLEFGLYEERIILGDEISPDTCRLWDKVSREKLDKDRFRRDM
                     GKEAEAYQEVVRRIEAVLA"
     gene            complement(18315..>18894)
                     /locus_tag="P5549_06285"
     CDS             complement(18315..>18894)
                     /locus_tag="P5549_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRW12716.1"
                     /translation="IRNLQRTDILEAEEPFKAGQKGSSAMPHKRNPMMSERVAGLSRV
                     IRGNATAALENVALWHERDLTHSSVERVIIPDSCILLDYMLEKFTSIVEGLVVYEDNM
                     ARNIETTLGLVFSQQMLLALVGKGLSREQSYSLVQGNAMRCWQEKIPFKELLLADETI
                     MSLLTADEMDKIFDYGIYTANVDYIFQRSGLC"
BASE COUNT         4445 a         5283 c         4247 g         4919 t
ORIGIN      
        1 gggtcggaga atccggacca atcagctccg gatgctgttc ttccaagcgg atgagctcac
       61 gcagcaaggc atcatattca gcatccgtaa ccagggggtc atccaacaca taatactggt
      121 attcatgttg gcggatttct tctcgcaatt catcaatctg tgactttatg tcaggcaccc
      181 ttaatacctc cagccttcag tttgcgatat ttttccctga cagtggcaat ataaatcgtc
      241 ataatgagcc gcatcaaata tacaaaaagc ttccataatc atatcataaa tcatatattc
      301 atggatgcaa aagaaaaggg gaagcagcca cctccccgta ccatttgctt attctctgta
      361 tcccattcca atactagtca ccttcgtaaa tttctgagcc cagctcttct tgcagataat
      421 gattcatatc aatctcttca ctgatcattg attttccctt cgtgatcact ttgatccttc
      481 ccactcaaag cgattgatct tgacagcatg ggttaccatc atctataacg caaaaaaatg
      541 attggtattg taaggagatg ttctgttatg gagaacatcg ttcagatatt taatgctttt
      601 attcttcttt tctgaatgtt ttcaatataa ttttctggac attaaaaagc ggttataaac
      661 aagctatgat gcaatcatct tgtttataac cgctctgatg cttaacaaaa gtttcttaac
      721 ttatcttttc caaaggagcg atgtcaaggc gcaaggtccg cacgcccgcc cgttcaaaat
      781 caaccacggc gatctgccct tcctgcagtt ggagcacaac tcccatgccg aacttccgat
      841 gaattacggt gtcaccttcg gccagaacac aggccgccgc cttttttaca tgcggcgatg
      901 tcagcagctg cgccggaatc tctttgataa aacgggaagg gggattgttt ctttcataac
      961 cgtataacag cctggaaatt gcattggtca gataaagcct ctcacaagcc cgggtgatcc
     1021 ccacataaca caagcgtctt tcttcttcca tgtcttcctc ggtttccgtc cgataggaag
     1081 gaaatactcc ctcttccatg ccggtcataa aaacgattgg aaactccagt cctttggcac
     1141 cgtgataggt catcatcatt acggtatcgg cattgttaac gtcatcggtg tcctgcacca
     1201 aggcaatgct ggccaaaaat tcgtccaggt cttcaattcc ttccttgctg aactccactg
     1261 ccagggaacg caattcctgc aggttttcta tgcgggtctg ggcatcggca gcgttgtttt
     1321 tgcgaagctc ttccacataa ccgctcatat ccatgacctg atccagaatc tccttcaatg
     1381 caacgccggc ttccttcaga gaaacgaagt attggatcat accgtaaaac tcttccagag
     1441 aagttgacat ttttctactg atcccaggca cacgggagat atcagccagc acttccatga
     1501 tcggcattcc ttcatgctgg gcaaattctt cgattttttc cagtgttttt tctcctatcc
     1561 cgcggcgtgg gacattgata atccgtcgca gactgagatg atcattggga ttgcaggtaa
     1621 gtttcagata agccaaaata tccttaattt ccttgcgctc gtagaacttg cgggcaccaa
     1681 caatttgata aggaatataa cgacgcagca aagcctcttc caaaatacgg gactgggcat
     1741 gggttctgta aaagacagca cagtcctgat agcgtttgtg atcccgatca accagatcgg
     1801 caatcgtatc ggccacgaac tgggcttcct gaaaactgtc gccggcacag aaataaatca
     1861 gtccctcacc ttccggcttg tctgtgtaca gttccttttc ttcccgatcc cggttgtttt
     1921 ttatcacggc attggcagct tttaaaatgg aaccggtcga acgatagttt ttttccagtt
     1981 taatcaaatg aacacgggga tagtctttca ggaatttctt gacgttgtag ggttcggccc
     2041 cccgccagct gtatatggac tgatcagggt cacccaccac aaaaatatta tgatgcgccg
     2101 ctgccagcaa attggcccat aaatactggg cataattggt gtcctggtat tcatcgatca
     2161 tgatatattg gaaccagttt tgatatttgg tcaggatgtc agggtattcc cgcagcagct
     2221 tgatggtaag aatgatcaga tcctcaaaat caagtgcatt gtactcccgc agacgagcgt
     2281 tgtaaatgcg aaatacccgg taatatttgt ctttaatatg caaaggcaga ttgagattgt
     2341 gaaagtatgg ttctgcatcc atcaggctgt ttttagcttg cttgatacta tagaggactt
     2401 cctcgggctt ggtctcgtaa tcccgctcct ctttcagtat ttccttgagc aaggtcttgc
     2461 cttcactgtc gtcaatgatg gcgaagttac gctcataacc cagcagatga atatccatcc
     2521 gcaggatctt gtaacaggca gcatgaaacg tctggatcca tttgccactg tagtccgggc
     2581 gcatgtccct tatgcgcgag cgcatctcct gggcggcttt gttggtaaaa gtaatggcca
     2641 gaatggcatc cggccttact ccattttcaa ttaaatagtt tattctttcc gtgagtaccc
     2701 tggttttgcc gctgccagct ccggccagca ccatacaggg tccatcccgg tgtaagatcg
     2761 cttcgatctg ggcttcattt aatccttcaa aattatgctt catatgggtt tgcctccgaa
     2821 ttagctctgt caatctgggt gtggtttctt tatcatcata acacaaaagg cccacgcaaa
     2881 ttcagcaaag atcgctgtca acttttcccg gccacttctc acgctttact gtcatttcct
     2941 gtttccaatt cacttcgtcc tggcagcctt tttcgtcccc aacagacttc ttcatcaatc
     3001 cttgaaatag ttgcatccgc tcccgtagga gcggatgctg ttttcggagt acgttcattt
     3061 ttttggccag ttgttagtcg gccagtgtta ccactatggt tttggtccac gtggcagtgt
     3121 ctgccgtagt tgaacgttca gggaactgca aggttgcggt aagggtgatt tcgttatcgc
     3181 cgtgctcctg catggctgca tataaagctt cttgatcaat cgtaaagctg ttgatggtgt
     3241 agtccaggca gctcgcataa tccttttcat tgggaatggc ccgggttatc gatcgggtcg
     3301 gcttgtttgt gtttccgggc catggcaatc ttatctgcgg cgcagggcta gttcgttgaa
     3361 gatgtcgtca ggaccaattc cccgttccac caacagaacc aaaagatgat agacaaggtc
     3421 agaagtttca tagattaatt ctgatttact gtcgttcttg gcagcgatga tgacctcagc
     3481 gttttcctcc ccgacttttt tcagaatctt gtcgatacct ttggaaaaaa gataggaggt
     3541 ataggagcct tccggcaatt gttctttgcg ctcccggatc aaattgtata attcaataag
     3601 aatgccgcga ttgctggcac tcgaatagat ttccttcggg tcaaacaact ggttttccac
     3661 ttcctgtccg gtgctgtccc gataaaaaca ggaataatga ccggtatggc aagctacgcc
     3721 ggtctgttcg actttgatca gcagggtatc cgcatcgcaa tcatagagga tctgtttgac
     3781 cttctgcacg ttccccgagc tttcgccctt catccacagc ttttgccggc tgcgggaata
     3841 aaaccaggtt cggccgctag ccagggtttt atcgagagac tcctcattca tgtaagccaa
     3901 catcagcact tgtccacttt gtgcatcctg aatgatagcc ggcaccagtc cgtgcgcatc
     3961 aaattttatg gtatctttca taatctcact gcaactcctt tacttgccag gtattccttg
     4021 gcctgggcga tagtatattc acgataatgg aatatggagg cacacaacac ggcgtccgcc
     4081 ttgccttcta cacatgcctc ccgcaggtgc tccagctggc cggccccgcc tgaggcaatc
     4141 accggaatgg gcagcctatc cgcaaccgca gcagtcagtt ccaaatcata gccgtccttg
     4201 gttccgtcct tgtccatgct ggtcagcaga atttctcctg cccccagctt ttcagcccgg
     4261 cgcgcccatt ccaaaacatc cagcccggtg ggagtgcgcc ctccgttgat atacacctcc
     4321 cacttccggc ccgttccctg cgcctcagac acatctgctt tccggcagcg ggcatcgatg
     4381 gccagaacaa tgcattgccg gccaaagcgg cgtgctgctt ccgtgatcag cgtaggatcg
     4441 tgtacggcag ctgaattgat ggataccttg tctgctccgg catttaatat ggcgcggata
     4501 tcatccaggg ttcgaatgcc tccgccgacg gcaaaaggaa taaatacttc ccgggcggta
     4561 ttacggacta catcaatcat ggtttgccgt tgatcagaag aagcggtaat atccaaaaat
     4621 acaagttcgt cggcaccttc ccggtcataa taggcggcca gttcgactgg atcaccggca
     4681 tcacgcagat tgacaaaatt gattcccttt accactcgac cggcgtgtac gtccagacag
     4741 ggaatgatcc tcttggccag cattactctt ctcccttcgc tgcctggatg gcatttttta
     4801 aagcaatttt tccgtcatat agtgccttgc caataatggc accgttgacg cccagcggtt
     4861 ccattttttt aaggcttttg atattttcca ggctggaaac gcctccggaa gcaataattt
     4921 tcaaacctga ctgttcagcc atttgctgaa tggcagcgag gttcggacct tgcagcaaac
     4981 catcccggga aacatccgta taaattgccg ttttcacgcc cagattgcgc atttgccggg
     5041 caaaatcgag gaccggcaaa cttgatttct ccacccagcc ttcgatggct accatcccat
     5101 cacgggcatc cagccccaga ataattttct ctgccccgaa gcggtacaac aggtcttcca
     5161 caaaagcagg actggttacc gcccgggttc cgataatcac ccggcgcgca cccatcgcca
     5221 agacattttc cacatcttcc aactggcgca atcctcctcc cacctgaaaa ggaatcgtga
     5281 tttgcgcggc aatcttctga atgacgtcat gattgccgca acgcccttga aacgcaccat
     5341 ccaggtcaac gacatgcagc cactgtgccc cctgttcttg aaaactggcg gccatcgcag
     5401 ccggatcaga ggaataaacg gttttctttt ccgctttgcc ttgaaccaga cgaacacact
     5461 gaccttcttt taaatcaatg gcaggataaa ttatcattat tgcatcgctc cgaaatttct
     5521 tagtacctgt aaacccatat cacttgattt ttcaggatgg aactgggtcg ccaccagatt
     5581 gcccttctcc accgcacaga caaacttgat gccgtaattc gcagcagcag ccatgacggt
     5641 atcatcctca ggaacggtgt ggtaggaatg aacaaaatag aaccagctgc cctgtggaat
     5701 tccggcaaag agctgactgc ctggtttgat ctcgatattg ttccaaccca tatggggaac
     5761 cttaaaagat gctggaattt gcagcttctc cactcggccg gcgatcacat ccagcccttt
     5821 atgcaatcca tcttcatcac tctcgctgaa cagcaattga taccccaggc atatgcccaa
     5881 cagaggagta ttgcgactga ccagttcatg aatagcctgg tctatgcctc gctcgcgtat
     5941 gtttccaatg gcatcggcaa aagcccctac tcccggcaga actaccctgt cagccgccag
     6001 aatctgttcc gggtcccggg tgatgatatt gtcatatcct attttggcaa aggcattgct
     6061 gacgctggcc agattgccca tgccataatc aatgatggcg atcaattttc gtccccctgt
     6121 tcacaacaac gttgcttgat tagagctgcc ccttggacga ccatgctcct tctaccccgg
     6181 caatgacagt cactgcttcc ttaaaggcac gggcaaaggc cttaaagaca gcttccacaa
     6241 tatgatggga gttgtcccct cgcaacagat cgatatggat cgtcatcccg gcctgattgg
     6301 ccaccgcctg aaaaaactcc ttgacgtttt ccgtacttaa attgcccagt tggggggacg
     6361 gaatttccac cgcatactgc ataaaggaac gtccggacaa atccataact actctggcca
     6421 gggcttcatc catgggaatg agcgcttgtc cgtaacgatt aatgcctttt ttttctccca
     6481 gcgcctcctt gatggcctgc cccaaacaaa tgccgatgtc ttccactgta tggtgatcat
     6541 cgacctgggt atcgccggtg gcgttcacta tcaggtcaca gcggctgtgc acggcgaaca
     6601 aggtcagcat gtgattcaga aaagggaccg gtgtttcaat ctgatgcttc ccgctgccac
     6661 cgacggtaaa cgacaggctg atttgggttt ccgctgtctg gcggacaatt ttgctgctgc
     6721 gtacgtccat ctctatttat cctttctaac tctcaggcag ttggcatgag cagtcaaacc
     6781 ctccacctca gccaatttga tgatatcgtc agcatcttcc tccagtcctt cgcgggaata
     6841 gtagataatg ctggaagctt tcataaaagt atccaccgtc accggagaat aaaaccgggc
     6901 ggtaccgccg gtaggcaata tatgattggg accggccaga taatccccca ccggttcagg
     6961 tgtgtgactg cccataaaga ttgccccggc attgtgaatt ttgctcagca tttcaaaggg
     7021 tttttccacc atcagctcaa gatgttccgg tgccaccaga ttggcgaccc ggcaggcttc
     7081 gttgatgtca tccaccagaa tcatcgctcc gtaattgctc aaggactgct cgatgattgc
     7141 gtacctcgac agaccggcca gttgttggtt gagttccttt tccactgcgt ccagcaaggg
     7201 ttcactgtcc gtcaccaaaa tactgcgggc cagtacatca tgttctgcct gggacatcat
     7261 atcggcagcc agataggccg gttctgcgaa gcgatccgca acaatcagta tttcactggg
     7321 gccggccagc atgtcgatat tgacatcgcc atacaccatt ttcttggcca acgtaacata
     7381 tatgttgccg ggaccactga tcaaatcaac cttcttgatc gtttctgttc cccaggctag
     7441 cgcaaaaaca gcttgcgctc cgcccatttt ataaatttcc gtcaaaccca gttctgctgc
     7501 cgccaccaag gtatagggat tcattttgcc gttggcatcc ggcggactga ccatggcaat
     7561 ttcctgcacc ccggccacca gggcagggat ggcattcatt aaaaccgagg aaggataagc
     7621 cgccgttcct cccgggacat agatgcctac ccgctccaac gggcgaaaca actgtcccag
     7681 cacgttgcct ctttcatccg gttccatcca ggagttgcgc agttgtttgc cgtgaaagta
     7741 atcaatatta tcgatggcct tgcgcaggga aaaaacataa tcatcatcga ccagttcgta
     7801 agcctcatcg atctcttctt ccctgaccag gaaattgtcg tggcttatct ctgccccgtc
     7861 aaagcgacgg ttgaactcaa agatggcttc atcgcctctg gccttgaccg cctgggcgat
     7921 ttcctgaaca cgctgttcgt attgctgcat gtcctcctgg tgactcccaa ggtaggcgag
     7981 tatttcttct tttgtctgca agcgcctgat tttcattgat tttttcctcc tttcacgatc
     8041 atctgcatct tttctatcaa aggctggact tcctggtact tggtgcggta actgacccgg
     8101 ttggccacca gccgggtggt cgaatccata atactgacca gttccaccag attgttttcc
     8161 cgcagggtcc gtccggaaga tacaatatct acaatcatgt ccgacaaacc catcagcgga
     8221 gccagttcga tattgccatg caaggtgatg atctccacct gcagtccatt gtcgcggaag
     8281 aatttttccg ccacagctgg aaatttggtg gccactcgtt tatagttcag ctcctgcagg
     8341 gtcaaatgct tcatatgagc cggtaccgcc accacaaagc gacagtagcc gaatcccaga
     8401 tcggccattt caaaaaggtc cttgccctgc tcgacaatga cgtctttgcc gacaataccc
     8461 agatcagctg caccgtgttc cacataagcg ggtacatcgg tgggacggca tataatatac
     8521 ttgatctgcg gatcaccgga aaaagtaaaa atcatgctgc ggctgtcttc agaaatgcct
     8581 tcacagggca atcccgcctg ctgcagcagc tcgatagtcg gttttaataa tgtccctttg
     8641 gacagggcaa tggttaaaat attatccatg ttgcctcccc ctatgattgc tcatccagat
     8701 acagcaggct gatgtcgccg cggccgcgca ctttttcctg caacgcctcc aggctgttgc
     8761 cttccacatc catttccacc gtcttgcctt caccgcgcag ttgctgtgct ttttccacca
     8821 gggcgcgcag gttgcgtccc cccagcagat accggttggt ttcctcttcc tgccgggcca
     8881 aaaccaaggc cagacggtcc atccccagcg caaaaccggt tgccggacaa ggaaagccga
     8941 attggcccag caaattatca tagcgtcctc cgcccagcaa gccataaccc agttcgggcg
     9001 aataaccttc aaagattacg ccggtgtagt aatcgagttc cagttcccgc aggacgccca
     9061 tgtcaatgac aatatgccgg gtaacgccat aatcctgcag ggcttcatag acccgcagca
     9121 aatcctcaac tgcctgggcg gtggctgcat gctgttccag atatggaatc ttttgtaata
     9181 cctccagccc gccatgcagg accggcaagc gggcaatgct ttccttccag gcatccggca
     9241 tgaccaggtt ttccagtcga cgttccagtt caaccaggtc cttcttctcc accagacgac
     9301 ggatctcgtc cttgtcttcg ctgtcaatgg cggcagcggt caacagaccg ttaaaaatgc
     9361 caatctggtt gatgctgatt ttaaaatcgt tcaagcccaa ttcctgcaac agcttgaccg
     9421 ccagactgat ggcttcggca tcggcccagg gtcctgatgc acccagcagt tcgatgccag
     9481 tctgccagaa ttcccggtat tgtgccagat gcggctgcac atggcgaaat acattggcgg
     9541 aataaaacaa gcgctgcgga tagatgctgt cgcgcagatg catggccgcc aaccgggcaa
     9601 tggaaacggt catttcagga cgcagggaga gaattccccc ttcccggtcc ataaaaagaa
     9661 acaatctttc ccggtgcttg ccggctccag tctcaaccac ttcgagaaat tcaaaggtgg
     9721 gggtcatgac ctccttgtag ccataagaag taaagatctg catggccttt tgctccagct
     9781 tgcgccggtt ttttacctct tgcggcagca gatcacgcaa tcctttaggt atttgtcgat
     9841 tatccatggt ttaactcctt gttcaattta tccagcacca ggcgatatcc ccgggcaccg
     9901 taattcagac agcgcttgac gcggctgatg gtagcggtac tggccccgct ctcccgggca
     9961 atcaccgaat aagtcgtgtc gttgtcaagc agccgggcca catgcatgcg ctgggccatc
    10021 gccttgatct ctgcaatggt gcatagatca tcgagaaagc ggcaggcttc ctcctcattt
    10081 tccagacaca acaacgcttg aaccagcagt tttgcattct cgtccatcca acgggcctcg
    10141 aaattcacca gatcacctca ttatttatcg ctattatttg ttctgcgtaa aaaagcttta
    10201 cagcagacgg gtatcgttta tcaccagttt gaattcgcat cccaggtatt ctgcggaatg
    10261 ctggcacatc atcattttgg tcaggaagat ttcgaagaac tccattaccg aacaaacact
    10321 ggtatcaatc tgcagttgca tggtgatgat ctgttcttcc ttctgcacga tgacgcggga
    10381 atcggtcacc gcataattga cccggtcacg cgcggtaaag gtggcaaaat cctgcttgcg
    10441 cacccgggaa cggttgacat cggatttgtc ggccaaggtt agggcagccg ccacatgatt
    10501 gacggcacag cctttggcat gttcttcatg gttgccgata gcgcccataa taatcgcgat
    10561 ttcttctgtc tccatgccca tttcattgag tacctgaaag gcaatcaagg cacctgacat
    10621 accgtgcccg tagcggttaa ccagattgcc tatgtcgtgc aggtagccgg caatagctgc
    10681 caattcagcg attctatctg gataacccaa ggcttgcaat atatcccggg ctagttggga
    10741 ggtcaattgg gcatgatgca aatcatgctc aatggcgccg atattgccca gaaaagtatt
    10801 gcccatatcc ataaaatcct tcactttttg attcgccttc accatctcca aggttaccaa
    10861 gcatcgtacc tcctcatcct cacctcatgc ttaacacatc gccggtgcct ataattccag
    10921 ccgcttgcgc aaatcgcgtg cttccaccgc cgcccgggtt cctggttcaa tattggcgat
    10981 gatctgccgg gcagcttcct ggtctccctg caccagtttt tgccgggcct cttccaactg
    11041 ccaggcagct tgctgctcct gacgggcaag attaatcttt tcttccattt tttcggtctg
    11101 tcccaattct tccaggcgtt gatatgcctc gatagcctgg tcatacttgc cttcagcaac
    11161 cagtgtaccg ccttcacgga taatttctcg cacttccact ctttgcggca gttgaaccag
    11221 catatatccg tataatccga tgactccgag caccaggatt ccacaggtga accaccacag
    11281 ggaatagcgt atctgccggc tcatcagcac atagaacaac aaaaaaggaa tgcccagaaa
    11341 aaaagttatt tttaaaaact cccgggtcga agcgaaaaac aacatgacga ccacaaccag
    11401 gaaaataaac attctaacat aaggggcttt attgaccgtc atcgtcaaac tcctttacct
    11461 tgatacagta ttaaagcatt tttttgataa agccgcaaca tttttttgca ttactacgct
    11521 tgacgcaaag ctttccgggc aatatcatga cgatagtgca tatctttaaa gtggattttt
    11581 ttcacttcgc tatatacttt ggcaatggcc gcggggattg aatcagcaca agctactaca
    11641 gccatgactc gtccaccggc ggttaccagt tgatcgtttt tcagcgccgt tccagcctgg
    11701 aataccaata tatcttcctg cagctgatcc aacccggtaa tggcgtcacc cttctgataa
    11761 gcacctggat agccgccggc cgccaagact acacccacgc atgcaccggc ctcgaactcc
    11821 acggttcgtc ccggcagttg gccttgggct gcagcttcta aaaccggcag cagatcactc
    11881 ttgatcatgg gcatcagcac ttgggcttcg gggtcaccga aacgggcatt atattccaat
    11941 actttaggcc cgtctgccgt aatcatcaac ccggcataaa gaaccccttg atagggacat
    12001 ccttcggctt gcatggcgcg aatggtcggc tcaatcactt ccgtaatgat ctggtgtttc
    12061 agttctcccg tatacagggg aggattggta taagctccca tgcctcccgt attggggcct
    12121 tcatcatgat catagatgcg cttgtgatcc tgagcagaaa gcaaggccag ataatctttc
    12181 ccgtcactga tggcgaagaa acttaattcc tccccttgca ggcactcttc caccaagatc
    12241 ttgtcgccgg ctgcgccgaa ggttttatcg ttcataatac tgtcaatggc tgcgctggct
    12301 tccgcaaaac actccgccac cacaacccct ttaccagctg ccagcccatc tgctttgacg
    12361 actgctttct gtccctgatc gaacagaatg cgggcatagt cacgagcctg ttccgcatca
    12421 tcaaagactt caaaacgggc ggtagggatt ttgtattttt taaaaaggtt tttggaaaaa
    12481 accttgctgc cttccaaacg tgccgcggcc gcactggggc caaaaatcaa caagccttcc
    12541 gcgctgaagc gatcagcaat tcccagtgtc aagggcgcct cgggacccac cacggtaaga
    12601 tcgatttcct tttcgagcgc aaattggcat aaggcgtcaa tatcatcaac ggccatctcc
    12661 acgcattcag ccagtttgct gatgccggga ttgccgggcg cggcataaat tttttccacg
    12721 ccagggctct gcgccagttt ccataccaag gcgtgctcac gcccaccgga tccaaccaca
    12781 agtagttttt tccccatttc gatctccctt caggctgatg tttttagtgt ttgaagtgac
    12841 gcatgccggt aaacaccatg gcaatgcaat actgattgca gacatcaatg ctttcctgat
    12901 cacgaattga tccgcccggc tgaatgatgg cggtaatgcc tttggatgca gccagttcga
    12961 cggtgtcgct gaaggggaag taggcatccg aggccagcac cgctcccttg tttttctccg
    13021 tatcggtcag cgcaattccg gccgagccaa cccggttcat ctgtccggca cccacaccga
    13081 tactcactcc gtctttggca gtaacaatcg cattggactt gacgtgtttg acgattttcc
    13141 aggcaaacag caaatcggta atttccgctg ctgtaggggc tttttcggtt accacctgca
    13201 gatctgcagc tgtcacgctt ccctgatcgc tctcctggac cacaaatcca ccttccaccg
    13261 atttgatctg cagttccggt gccgaactga tttccatctc cagcacccgc aggttctttt
    13321 tggcctggaa gatggccagg gcatcatcat cataaccggg ggcaataatg cattccataa
    13381 atgtctgcgc agctttgcgg gcggtggcag catccacctg gcggttgaag gcgataatcc
    13441 caccgtaagc agaaaccggg tcggcattaa atgccttttc ataggcctct tccagcgtat
    13501 cggcaatagc tgtgccgcat ggattggtgt gtttaataat cacacaggca ttctgttcaa
    13561 attccttgac cagagcccag gctgcctgca catcgataat attgttgtag gacaattcct
    13621 tgccgtttaa caccttggca tcccccagac tggctccggg tgtcatggta cggtagaagc
    13681 aggccttctg atgagggttt tccccatagc ggagttcgta catcttttct ccggcgaccg
    13741 tcatgttgtc aaggaaggta ttcccggtca atttggccaa ataagctgag atcatggcat
    13801 cgtaggcggc cgtatgggtg aaagcctcca gggccagctt cagcctcatt tcccggctaa
    13861 cctgaccact ctgctgcaat tcttccaaaa ccgggccata aaactccggt ttgacgacaa
    13921 tgatcacatc tgcatggttt ttggccgcgg agcgcaccat gctgggaccg ccgatgtcga
    13981 tattttcaat ggcttcttcc accgtgaccc ccggtttgct gacggtttcg cggaaaggat
    14041 aaaggttgac ggcgacaata tcgatgggat caatctggtt ggcatccaac tgctgcaaat
    14101 ggtcgggaat acggcgggcc agaattccgc catgaatctt gggatgcaga gttttcaccc
    14161 ggccatcgag aatttccggg aaaccggtta tatcagcgac tttcatcacc ttaaccccgg
    14221 catcggccaa ggttttgtag gttcctccgg tcgagatgat ttcatagccc aatttctcca
    14281 gcccttgggc aaattcaacc acaccgtctt tgttggaaac actgatcaaa gctcttttca
    14341 tgtttgttcc tccttaccct tgcataaata ctgtcaatta tgatttacca atacttttct
    14401 tccttctata tacacccggc cttcggcaaa ccactgcagg acccgggcat aaagcttgtg
    14461 ctcctccacc aggatgcgtt cggccagatc atcttcgcta tcgtcaggca aaaccggtac
    14521 caccgcctga gcaatgatcg gaccggtgtc catgccgtca tccaccaaat ggaccgtaca
    14581 accgctcaat ttgaccccgt atgcaactgc ctggcgctgg gcatgcagcc cggtaaaagc
    14641 aggcagtaaa gcgggatgaa tatttaaaat ccggtgggga taggcgtgca gcagcacctt
    14701 gcctaccagc cgcatatacc cggccagcac gaccagatcg atttccctgg cttgcaactc
    14761 tttgacgatg tcgctttcat agccggcccg atcagagaaa aggcggggat cgataaaaac
    14821 atgggggatt tctgcttctt gcgccttttg cagggccaac gcgtcttcct tatcgctgat
    14881 caatacctca atgcaggcat tcagttggcc ctctttgatt gcctccaaaa tcgctaaaaa
    14941 attgctgcct cggcctgaag ccagtatggc cagacgcaga cagcccggtt ccattatcct
    15001 tttcattagg caatcacgac ctttgattgt cgggcggtga tctcaccaat tattataggc
    15061 tgttcctcct gggcacgcaa gcactttaac gcgttatcac actgttgtgg gtcaactgcc
    15121 agaatgaagc ctatgcccat attaaaagtc cgaaacattt ccgccggtgc cacctcgccc
    15181 atgcgggcga tcatctggaa aatcggcggt atggcaatag cctccgcctt gatctccgcg
    15241 cccagaccag ccggtatgct tctcattaag ttttcagcaa tcccgccacc ggtaatatga
    15301 gccatgcctt ttacttccac ttcctgcagc aaagccagaa ccgacggtac ataaatacgg
    15361 gtgggagtga ttagttcttc gcccaacgtc ttgcccagtt catcaaagta atcatcgagt
    15421 ttcaatccgg cgctttccag caaaaccttg cgcaccagag aataaccatt ggagtgaatc
    15481 ccggaagaag gcagaccgat caacacatcg ctgtctttca tggcctcacc gttgattagt
    15541 ttgggtttgt ccacgacccc cacggtaaaa ccggccagat catactcatc ttctttataa
    15601 aatcccggca tctctgccgt ctcgccgccg atcagcgcac agcctgcctg gacacagcct
    15661 tcactgacgc cctggaccag cgcttccacc agttggggct gtagtttgcc gatggcaatg
    15721 taatccagga aaaagagcgg ctcggctccg tgcaccagga tgtcattgac gcacatggcc
    15781 accagatcga tgcccaccgt atgatggacg tccatcatct gggcaatgcg cagtttcgta
    15841 cctacaccgt cggtgcccga aaccagaaca ggttccaaat atttttgctg gtttagggca
    15901 aagaatcccc caaaagaacc gatatcggcc agcacctcgg gacgtcgggt ccgacccacc
    15961 cactttttaa ttcgttccac cgaatcattg gcagcgtcaa tgtccactcc cgcttttttg
    16021 tagctcaaac cttctttatc catgtttccc tccaaatata ctttttctat acttccggca
    16081 atgctttgcc cgccggatat tctccgctga aacaggccat acaatagctg tcattctcat
    16141 tttgcaaagc tttgaacaaa cctgcccggc tgatataatg cagactgtca gctccaataa
    16201 acgcccggat ttgctcattg tccatggtat tggcaatcag ttcctccctg cgggaagtat
    16261 cgatgccgta ataacacgga aatttagtag gcggtgaggc caccaccata tgcacttctg
    16321 atgccccggc tgttcgcagc atttgcacga tctttttact ggtcgttccc cgcacaatgg
    16381 agtcatccac catgattacc cttttgccgc caactacatc cggtacggcg ttgagcttta
    16441 aacgcacgcc cagttcacgc atttgctggg tgggctgaat aaaagtacgt cccacatagc
    16501 gattcttcat caggccttct tcaaaggcca gaccggattg ttcggcaaag cccaaggcag
    16561 aagcggtacc tgagtcgggt acggcaatca caatatcagc gtcgatttta acctcccagg
    16621 ccaattgctt gcctaattcc cgccgcgccc ggttgacatt aatcccatcc agaacactgt
    16681 cgggacgggc aaaatagata tattcgaaaa tacagtgggc caggcgatta gcattgccca
    16741 actgcgcgga gtgcattcca tccttttcaa tcaccacgat ctcgccggcc tgcacatccc
    16801 ggataaactc tgctcctacc gtatccaagg cagccgattc tgaagacagc acgtagtcac
    16861 cattcaagct gcctaaacat aaaggtctga ttcccaaggg atcgcgtaca cccaccagcc
    16921 ggtcctcggt cataatcacc agggcaaacg ctcctttgat atcaatcatg catttggcca
    16981 aggcatctgc cagtttgtcc tgggaatagc gcgccagcaa attggcaatc acttccgtgt
    17041 cagtggtggt ttggaaaacc gaaccatagg tggccagcct ttggcgcagt tccgcggcat
    17101 tggtcaggtt gccgttgtgt gccagagcca ccgtgccgtt taaataatga aaaaccagcg
    17161 gctgggcatt gaccatggca ctgcctccgg tagttgaata gcgcacgtga ccaacggcga
    17221 tatttccttc caattcttca atatgacggg ctttaaccac atcagaaacc agtcccatgc
    17281 ccttgtgcaa ttgaatatct tgaccgttgg caacagcgat gccggcactt tcctgcccac
    17341 gatgctgcag ggcatacaat ccgtaaaaag tcgtacgggc agcatcggtt tgatggacag
    17401 gattattcat gaaaatcccg aacacaccgc attcgtcctg ccatttaccc tcacttgccc
    17461 aatcttcaag ttcatataac atacgatagc ctccgtccaa aactcacgga acagctccgt
    17521 tgccgtccta acggtataat taaaatgtgt gttctaggcc aataccgctt caatacggcg
    17581 caccacttcc tgataggctt ccgcctcctt gcccatatcg cggcggaaac gatccttgtc
    17641 cagtttttcc cgactgactt tgtcccagag gcggcaggta tcgggggaaa tctcatcgcc
    17701 caggatgatc cgctcttcat agagaccgaa ctccagttta aagtccacca ggataatgcc
    17761 gatgctatcg tagaaagctt tcagtacctg gtttatttgc tgcgcctgcc ggtcgatctc
    17821 ctgcaactgc tccgctgttg cccagcccat tacccgggca tggtattgat tgatcatggg
    17881 gtcatgcaat tcatcatttt tataataaag ctctatgatg ggctcgccca gttccacacc
    17941 ttcctccagg cctacccgtt ggctcaggct gccggcaatg acattcctga cgacaacttc
    18001 cacggggatg atttgcacct ttttcaccag tgtttgccga tcatccagct gctccagcag
    18061 atgggtttca atttcatgct tctccaataa tttgaacagc agattggaaa cccggttgtt
    18121 gacgattcct ttgtttaaaa ttgtgccttt tttctgcccg tcaaaggcag tcgcatcatc
    18181 tttgtattta atcagaacaa ctttttcatc gtcggttgca taaatttgct tggctttgcc
    18241 ttcataaagg aattctcgga cctgcatgcc atcatcctcc taaaatcaat ccccggcaat
    18301 ataacacact gagcttagca gagcccgctg cgttgaaaaa tataatccac gttggcagtg
    18361 taaatcccgt agtcaaatat cttatccatt tcgtcggccg tcagcaggct catgatggtt
    18421 tcatccgcca gcagcagttc tttgaaagga attttctcct gccagcaacg catggcattg
    18481 ccctgcacca gactgtatga ttgttcccgg gacaaaccct tgcccaccag ggccagcagc
    18541 atttgctgtg aaaataccaa tccgagtgtc gtttcaatgt tgcgcgccat attgtcctcg
    18601 tataccacca ggccttccac aatgctcgta aatttctcca gcatatagtc gagcagaata
    18661 cagctgtccg gaatgatgac ccgctcaacc gaggaatggg tcaggtcccg ttcatgccag
    18721 agagcgacat tttccagagc agcggtggca tttccgcgga tgacccggga aaggccggct
    18781 actcgttcgc tcatcatggg attgcgttta tgaggcatgg ctgacgagcc cttttggcca
    18841 gctttaaacg gctcctctgc ttccaggata tcggtgcgct gcaggttacg gatc
//
LOCUS       DAOTHZ010000041        18534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3602_length_18534_cov_7.323827, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000041 DAOTHZ010000000
VERSION     DAOTHZ010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_18534_cov_7.323827"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="P5549_06290"
     CDS             <1..1035
                     /locus_tag="P5549_06290"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HRW12717.1"
                     /translation="AMDKFFAIMERFAPEAVEIMVTRYQLLRHVLYNQPIGRRQLTRT
                     MGLTERTVRSELDLLKSKGAIKSTGAGIYLTDFGYEMLRDIESMVPGLFHIKSLAEKI
                     KRRFGLREVIIVPGDSNQDPYAMDDLGRAAAEYLHSSLYPGCVVAVTGGTTLAGMARA
                     MRAGVEQAETICLVPARGGLGENVETQAGNIAAKIAQRIGAQYRLLHIPDNLEKSTAE
                     ALKKDIHVKEVLDTIKSVRILLHGIGPADEMAERRGLSAKEIEFLQENKAQGEALRYY
                     FSQMGKIIFEIPGIGLELEDIQHIETIIAVAGGSNKASAIAAVLKSGFDHILITDEGA
                     AQKIIYGRDE"
     gene            1038..2042
                     /gene="gap"
                     /locus_tag="P5549_06295"
     CDS             1038..2042
                     /gene="gap"
                     /locus_tag="P5549_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRW12718.1"
                     /translation="MSVKIAINGFGRIGRLVARAAVQREDIELVAVNDLGDPEGGALL
                     FKYDSIHGKFPQEVKLEENTIVAGNEKFAYLSERDPLKLPWKDMGVEVVVEATGKFRS
                     KEKASLHLQAGAAKVIISAPGKNVDLTTVMGVNNDIYDPLNHHILSNASCTTNCLAPV
                     AKVLVKEFGIVRGMMTTIHSYTNDQRVLDLEHDDMRRARAAALSMIPTTTGAASALGE
                     VIPELKGKLDGLAIRVPTPNVSLVDLTVELEKAASVAQVNQAFRQAEETYLQGILRYT
                     EEPLVSRDYNGETCSAVVDGPLTMAMGDNLVKVVAWYDNEWAYSLRVIDLVAYIAGRK
                     "
     gene            2089..3264
                     /locus_tag="P5549_06300"
     CDS             2089..3264
                     /locus_tag="P5549_06300"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRW12719.1"
                     /translation="MRSIRDIDLRGRRVLMRVDFNVPMDKAGDITDDARITAAIPTIQ
                     YIVGQGARLVLMSHLGRPDGQRNEKYSLKTVAHHLSELLKQEVRMADDCIGAQVQAAI
                     VELKSGEIILLENVRFHPGEEENDADFSKQLAMLGDIFVNDAFGTAHRAHSSTAGVAA
                     FLPSYAGFLLENEVKMLRQVMEHPESPRVAVLGGAKIKDKLGLISNLLDKMDILLMGG
                     GMANTFLKAQGVNIGKSLCEDKLLEQARNLLDKAEKQNKKIILPMDAIAAETLSEDTV
                     ATVCEINAIPENMMILDIGPQTTAVFSREIGRAHTIIWNGPVGAYEYAQFTAGTDAIA
                     RAMAVSQAVTVIGGGDSAAAVHKLGLDQEFTHISTGGGATLEFLEGKTLPGVQSCEG"
     gene            3282..4046
                     /gene="tpiA"
                     /locus_tag="P5549_06305"
     CDS             3282..4046
                     /gene="tpiA"
                     /locus_tag="P5549_06305"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRW12720.1"
                     /translation="MRKKIIAANWKMHKNRSEAQDYVNAFLPLWHEIKAADTEVIICP
                     PFTILHTLQTALQGSTVMTGAQDMYWEKHGAFTGEISAPMLTDCGCSHVIVGHSERRH
                     LLGETDDHINLKIKAALAGSLIPILCVGETLEERQNDQAFQVVQEQLDHALFEVDLAT
                     RNLIVAYEPVWAIGTGVNAGSEDAQDMIAFIRGQLFRQFNQELADITPILYGGSVKAA
                     NMREFIVKKDIDGALVGSASLDPGEFAEIARAVKNV"
     gene            4039..5580
                     /gene="gpmI"
                     /locus_tag="P5549_06310"
     CDS             4039..5580
                     /gene="gpmI"
                     /locus_tag="P5549_06310"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRW12721.1"
                     /translation="MSKAPMVLMILDGWGHREDQQDNAISMAHPQNFYNLWDAYPHTL
                     LECFGNAVGLPRGQMGNSEVGHLNIGAGRIVFQEITRITQAIEDRSFFTNPVFLKAMD
                     DARCHNGAVHLLGLVSDGGVHSDLTHLFALLELCKDQGIDSVFVHAFLDGRDVAPQSA
                     MEYISLLENFMRQLGLGRIASVGGRFYGMDRDNRWERIEKAYQSIVLGEGLQAAKAQA
                     AIERSYEARVSDEFMEPVVIVDEQGQPIGRISDGDSVIFFNFRADRARQISHSLLDKT
                     MDRFERKIVPAIHYVCMTQYDAGLAAPVAFPPQNLDNTLGEVLSAAGLKQLRIAETEK
                     YAHVTFFFNGGVERANPGEDRVLIPSPQVATYNLQPEMSAFEVTQRVLQEIDRDFYDV
                     VIMNYANPDMVGHTGVLEAAVKAVKTIDDCLIQVISRVQEKNGQVIITADHGNCEMMT
                     DAVNGCPFTAHTTNKVPFILVDDRHQGKTLREGSLRDVAPTMLGLLGLPAPDEMTGNT
                     LLSME"
     gene            5621..6901
                     /gene="eno"
                     /locus_tag="P5549_06315"
     CDS             5621..6901
                     /gene="eno"
                     /locus_tag="P5549_06315"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRW12722.1"
                     /translation="MSIIVDVYAREVLDSRANPTVEVEVILEDGTMGRAIVPSGASTG
                     AHEAVELRDGDPKRYGGKGVLKAVENVNEIIAPELIGMDVTHQIDIDTLMIELDGTPN
                     KSKLGANAMLGVSLAAARAAAEYLGLPLYQYIGGTNAREIPVPMMNILNGGKHADNNV
                     DIQEFMVVPCGAPTFAEALRMGVEIYHSLKKVLGSKGLATSVGDEGGFAPNLASNEAA
                     IEVILEAINQAGYVAGQDVFIALDPAASEFFNDGRYTLNQKSLSSEEMVDFYEGLVNR
                     YPIISIEDGLAEDDWDGWKIMTDRLGRKIQLIGDDLFVTNTKRLAMGFEKGVCNSILI
                     KLNQIGTLTETLDTIEMARRRGYTTVISHRSGETEDSTMADVAVAVNAGQIKTGAPAR
                     TDRAAKYNQLLRIEEELDEFARFNGRKVFYNLNN"
     gene            6984..7208
                     /gene="secG"
                     /locus_tag="P5549_06320"
     CDS             6984..7208
                     /gene="secG"
                     /locus_tag="P5549_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRW12723.1"
                     /translation="MLKIIVLILQTLCALGLIATVLLQSGKSAGLSGSIAGGGEAIFG
                     KKKGVDELFAKITAYLSALFFVLTLALAVI"
     gene            7372..9813
                     /locus_tag="P5549_06325"
     CDS             7372..9813
                     /locus_tag="P5549_06325"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRW12724.1"
                     /translation="MKLETVKKTWMTILSSLVILLLTPAMALASEADLIIPELSASQN
                     SILVAGIAVCVLGMIFGFYQFQKVKKLQAHQVMLDVAQTIYETCKTYLIQQGKFLAIL
                     FVFIGVCIAFYFGFLNQMEIGNVLFILMWTIIGILGSYSVAWYGIRMNTLANSRVAFT
                     SLERKPLKLLNIALDAGMSIGVLLVCVELIMMLIILLYVPRELAGASFIGFAIGESLG
                     ASALRIAGGIFTKIADIGADLMKIVFGIKEDDPRNPGVIADCTGDNAGDSVGPTADGF
                     ETYGVTGVAMVTFIVLAVAPALQTTLLAWIFTIRVLMVITSVLSFYINRAYSQVKYGS
                     ADDFDFEAPLTSLVWITSVISIIVTFGVTYYLVGPGAAEATALTPLWVVLAAIISLGT
                     LAAALIPEFVKVFTSPKSKHVAEVVKASREGGPSLNILSGLVAGNFSAFWIGMVFVAI
                     MFGAYLASQPIPAEIMSYSSIFAFGLVAFGMLGMGPVTIAVDSYGPVTDNAQSIYELS
                     LIEEVPNVAKNIEKDFGFKPDFDMGKYYLEANDGAGNTFKATAKPVLIGTAVVGATTM
                     IFSLILVIKNVLGVDPATILTLLNPYTILGFLCGGSVIFWFTGASTQAVTTGAYRAVA
                     YIKKNINLEEAADVKANTEKSKEVVQICTQYAQSGMFNIFIAIFSFALAFACLSAPSN
                     GNNEPVAFFVAYLISIAVVGLFQAVFMANAGGCWDNAKKVVEVDLKEKGTMLHEATVV
                     GDTVGDPFKDTSSVALNPIIKFTTLFGLLAMEIAISETFRNVAPYVGAVFLLIALFFV
                     WRSFYGMRIPVGDDK"
     gene            complement(10036..11064)
                     /locus_tag="P5549_06330"
     CDS             complement(10036..11064)
                     /locus_tag="P5549_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRW12725.1"
                     /translation="MKIALLSPIAWQTPPLHYGPWENMVSLLAEGLQQEGIKVSVFAT
                     GDSASANLEWVCPHAYEEDRSLDPKVWECLHIAHLFEQADRFDLIHNHYDFLPLTYSR
                     LISTPVLTTIHGFSSPQILPVYKKYNTSADYVSISYADRHPDLHYTANIYHGLDLEQF
                     RFEKQSGEYLLYFGRIHPDKGTAEAIDIARKSRLPLILAGIIQDQKYFLTQVAPALDE
                     ANIRYVGPVGPKYRNELLGGALALLHPIHFAEPFGLSVIEAQACGTPIIAFNKGSMPE
                     VIVDGVTGYLVNDTASAVIKIKEISNLNRLDCRLWVEERFSRQRMVADYLDVYEQILN
                     AAAPSLLS"
     gene            11394..12752
                     /locus_tag="P5549_06335"
     CDS             11394..12752
                     /locus_tag="P5549_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12726.1"
                     /translation="MANTYLQEYRQKLITAEQAASLVNSNDRILYAVFAGRPTDFDIA
                     LSQRKEELFNVQITQCGGNNIGPDSSAFSDPSRQHFTNNSWFFDGVDRQLHDQDMMYY
                     HPCQFGQLQDIIGSDLFPYDIYVQKVSPMDHHGYFSFGLSNVNSLESCLGAKKVILEV
                     NENMPRVPGGSEDAVHISMVDHIIESANAPLLVLPPSREPSPADVTMARLLLPEIPDR
                     ACLQLGIGALPNLLGDLLCDSDLQDLGIHTEMFVDSMVNLFSSGKVTNRYKTTDRCKI
                     AFTFSIGSRSTYDFMENNPLMASHCGRYTNNARLIATNDNFISINNTVMMDLFSQACS
                     ESSGTRQISGTGGQIDFINGSWFSRGGKSFLCLTSTYRDKNGQLQSRIVPTLPSGSIV
                     TTARTFIDYAVTEFGIVKLKGKPTWHRAEQLISIAHPQFHDDLVKEAQKMKIWRRSNK
                     IE"
     gene            12886..14469
                     /locus_tag="P5549_06340"
     CDS             12886..14469
                     /locus_tag="P5549_06340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRW12727.1"
                     /translation="MYSQTFANMGLKPRLLQMIENKGFEIPTPIQIKAIPPALAGHDV
                     MGQAQTGTGKTAAFGIPMLNRMVPGRGLQGLVVCPTRELAVQVTEEINSLGKGMNLRS
                     LAVYGGQSIELQIRALSRRPELLVGTPGRLLDHMQRGNINLSNLSYLVLDEADEMLDM
                     GFLPDIERIMEACSHDRQTFLFSATLNEEIRRLGSQFMHDPDIILIESLELTVPLTRQ
                     FYLEVSPRYKIATLCRLLEVEQPTVSLVFCRTKKGVDMLAHALNDRGYDADALHGDMT
                     QRERDNVMERFRKGNVTILAATDLAARGLDIEMVSHVFNFDLPEDPDSYVHRIGRTGR
                     AGRDGIAISFVEPRQMQQIKLIEHHIGKKIMRRHPPSSRDAVESRRESLNRRLLETAR
                     EGGLEGYQQMAGTLLTENDAQTLLAAALKIIAGGEPEMELAERKTNSLDTAHVEMPLG
                     RMQGMNPRRLADYLCANTTLTPRQVGDIEIHSNTSYVEVPMDKIDEAYEALAGYERNR
                     KSGHPRFGLSRSGRNKNIN"
     gene            complement(14532..14774)
                     /locus_tag="P5549_06345"
     CDS             complement(14532..14774)
                     /locus_tag="P5549_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HRW12728.1"
                     /translation="MQDKEMVNDILSSVKSSLATYAQVIGETSNQQLRQTIQQIRNSD
                     EQFQYQLYQMAEQKGFYKAAAQADQSDIQTVKSQLG"
     gene            complement(14804..14986)
                     /locus_tag="P5549_06350"
     CDS             complement(14804..14986)
                     /locus_tag="P5549_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12729.1"
                     /translation="MTVNQMELQNLRHLIGGHTTAEKKLRFYASQCQDAQIKQMFDQA
                     AQSAMNTRNKLISFLQ"
     gene            complement(15186..16502)
                     /locus_tag="P5549_06355"
     CDS             complement(15186..16502)
                     /locus_tag="P5549_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRW12730.1"
                     /translation="MQINKMSAPQLLVGSFLVLILLGSLLLYMPWAANTPTRYVDALF
                     TSATSVCITGLVVVDTGTHWTLFGQIIILLLVQVGGLGVMAFAGFFAMLMGRKIQLRE
                     RLLMQRSINVSAVGGIVRVFRYLLGFTFLLEALGAVILAIKWMPLMGVKKALWHGVFH
                     SISAFNNAGLDLFGNFKSLTDFSHDITINIVFSLLVMIGGLGFFVCYELYNYRQSRKL
                     SLHSKVVLTTTGILLVLGTFLLLAVEYNHALKALTPGHKLMVAFFQSISSRSSGFATI
                     DINSLLLPSQLLLIVFMFIGASPGSTGGGIKTTTVALMFAVVVAQLRGKKDIEIFERR
                     IVSSDVYPSFTLFFMAIFMLIIMVFLISLTHPGDFMKILFDVTSALGTVGFSTGLCLE
                     LNAFGKILIVICMFLGRVGPLTLGFALAYKKKQPEIHYAQGKIMIG"
     gene            complement(16523..17179)
                     /locus_tag="P5549_06360"
     CDS             complement(16523..17179)
                     /locus_tag="P5549_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRW12731.1"
                     /translation="MKNKQFAVIGIGRFGESLVKELTRLGYEVMAIDIDEEKINDTVD
                     IATHAVQADSMDEDTLKALGIRNFDVVIVAIGNNIQSNILTTIMLKELGVNKVVAKAQ
                     NALHGKVLEKIGADLVIYPERDMAIKLARSLVSNNFLEQINLSPTHSVMEFFTPVTLA
                     DKSLVETGLRKKMGVTVLAIRRNDDVIVAPGPEERLRKGDVIVALGSNDDLEYITNLD
                     "
     gene            17691..18461
                     /locus_tag="P5549_06365"
     CDS             17691..18461
                     /locus_tag="P5549_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12732.1"
                     /translation="MSYEYQIVDNIVIFQFNRGKLNSITMETLQGLNEVIDRVNQENE
                     LKGIILTGQGRYFSGGFDLGTFTSFQSKEEILDWFNYEEEVLFKLFTCSKPVVAAING
                     HATAMGMILSMACDYRLVINNPKIKLGMTEIKIGLSLTLVQGAVMRFGLDTDKKYRDV
                     IYKGELFAPDYAVAQGIFDELAENEEELLEKAKAKVVSMIDTPGRPFIILKELEKMQT
                     VDWLKGKLKEVDWGPLVATFTDEKVVNTLKMVKAALGE"
BASE COUNT         4940 a         3989 c         4745 g         4860 t
ORIGIN      
        1 gcaatggata aattttttgc aattatggaa cgcttcgctc ctgaagccgt ggaaatcatg
       61 gttacacggt atcagcttct caggcatgta ttgtacaacc aacccatcgg cagacgccag
      121 cttaccagaa ctatgggttt gacggaacgg acggtgaggt cggagttgga tttgctgaaa
      181 agcaaagggg cgataaaaag taccggggcg ggcatttatt tgactgattt tggttatgaa
      241 atgttgcgcg atattgaatc gatggtgcca ggattgtttc atatcaagag cctggctgaa
      301 aaaataaaaa ggcgttttgg cctgcgggaa gtgatcatcg taccgggaga ttccaatcag
      361 gatccctatg ccatggatga tttgggccga gctgcggcag aatatttgca ttcttccctt
      421 tacccgggtt gtgttgttgc cgtgaccggt gggaccaccc tggcggggat ggctcgcgca
      481 atgcgcgccg gggtggaaca ggcggaaacg atttgcttgg ttcctgcccg gggcggtttg
      541 ggcgaaaacg tggagaccca ggcaggcaat attgctgcca aaattgccca aagaattgga
      601 gcgcagtatc ggttgctgca cattcccgac aatctggaaa agagtaccgc ggaagcgtta
      661 aaaaaagata tccatgtaaa agaagtgctg gacacgataa aatcagtccg cattttgttg
      721 catgggatcg gaccggctga tgaaatggct gagcgccggg gattgtctgc taaagaaata
      781 gagtttctgc aagaaaacaa agcgcagggt gaagccttaa ggtattattt cagccaaatg
      841 ggcaaaataa tatttgaaat cccgggaatt gggctggagc ttgaggatat tcaacacatt
      901 gaaacgatta ttgcggtagc cgggggcagc aacaaggcat ctgctatcgc agcagtatta
      961 aaatcaggat tcgaccacat ccttattacg gatgagggtg cagcacagaa aataatatac
     1021 ggaagggatg agtgagcatg tctgtcaaaa tcgctattaa tggttttgga cgcatcggaa
     1081 ggttggttgc ccgggccgcc gtgcaaagag aagatattga actggtggcg gtaaacgact
     1141 tgggcgatcc tgaaggcggg gccttgcttt tcaagtatga ttccatacac ggaaagtttc
     1201 cgcaggaggt taagctggaa gaaaacacga ttgttgccgg aaacgaaaag tttgcctatc
     1261 tgtctgaacg cgatcccttg aaactgcctt ggaaggacat gggagttgag gtggtggtcg
     1321 aggcgaccgg aaaatttcgc agcaaagaaa aagcttccct gcatttgcaa gcgggagccg
     1381 ccaaggtcat tattagtgca ccgggaaaaa atgttgattt gacgacagtg atgggtgtta
     1441 acaatgatat ttatgatccc cttaaccatc atattttatc caatgcatcc tgtacgacca
     1501 actgtctggc accggttgcc aaggtgctgg tgaaagagtt tggtatcgtc cgcgggatga
     1561 tgacaaccat ccattcctac accaacgatc agcgggtgct tgacctggaa catgatgata
     1621 tgcgccgcgc ccgggctgct gccttgtcga tgattcccac tacaactggt gccgccagtg
     1681 ccctgggtga agttatcccc gaattaaaag gcaaattgga cggactggcc atcagagttc
     1741 ccacgcccaa tgtttctctg gtggacctaa ccgtagagct ggaaaaggcg gcttctgttg
     1801 cgcaagtcaa ccaggctttc cgtcaggcgg aagaaactta tttgcagggc attctgagat
     1861 atacagaaga accgctcgta tcaagagact ataatggaga aacttgttcg gcagtcgtag
     1921 acggaccgct gaccatggcc atgggagaca atctggtcaa ggtggttgcc tggtatgaca
     1981 atgaatgggc ttattccctc cgggtaattg acctggttgc ctatatcgca ggtcggaaat
     2041 aatctgccaa accataaata ttcaattgtc gcgggggtgt atctaatttt gagaagcatc
     2101 agggatattg atttaagagg ccggcgggtg ttgatgaggg ttgactttaa tgtgcccatg
     2161 gacaaagcag gggatattac cgatgatgcc cgcataaccg cagcgattcc gaccatccaa
     2221 tacatcgttg ggcagggagc acgattggtt ttaatgagcc atttgggcag accggacgga
     2281 caaagaaatg aaaagtatag tcttaaaact gtggcacatc atctgtctga gctgctcaag
     2341 caggaagtca ggatggctga tgattgtatt ggagcgcagg tgcaggcagc cattgtggag
     2401 ttaaaaagcg gagaaatcat cttgttggaa aatgtgcgct ttcatcccgg agaagaagag
     2461 aatgacgctg atttctcaaa gcagctggct atgctgggtg acatctttgt caacgatgca
     2521 tttggaactg cacaccgggc gcattcttcc acggcagggg tggcagcatt tttaccttcc
     2581 tatgccggat ttttgcttga aaatgaagtg aaaatgctgc ggcaggtcat ggagcatccg
     2641 gaaagtccaa gggtggcagt tctcggtgga gccaagataa aagataaatt aggacttatc
     2701 agcaatctcc tggacaagat ggacatcctc ttgatggggg gggggatggc caacacattc
     2761 ctcaaagcac aaggtgtaaa tattggcaaa tccttgtgtg aagacaagct gttggaacag
     2821 gccagaaacc tgctggacaa agcagaaaag cagaataaaa aaatcatctt gccgatggat
     2881 gcgatagcag cggagacatt gtcggaagat accgttgcca cagtatgtga aatcaatgcc
     2941 ataccggaaa acatgatgat cctggatatc ggtccgcaaa cgaccgcagt tttttcccgg
     3001 gaaattggtc gtgcgcacac tattatctgg aatggaccgg tgggtgctta tgagtatgcc
     3061 caatttactg caggaacgga tgcgattgcc agagcgatgg ctgtctccca ggcagtaaca
     3121 gttattggtg ggggtgattc agcggccgcc gtgcacaagt tggggcttga ccaggagttt
     3181 acccatattt ctacaggggg aggggcaacc ctcgaatttt tggagggaaa aaccctgcca
     3241 ggcgtacaat cttgtgaagg gtgaacaaag gagatggggt attgagaaag aaaattatcg
     3301 ctgccaattg gaaaatgcat aaaaacagaa gtgaagcaca agattatgtc aatgcttttc
     3361 tgccgttatg gcatgagatc aaagcagcgg acaccgaagt gatcatttgt ccgcctttta
     3421 ccattctcca tacattgcaa accgccttgc agggcagcac ggttatgact ggggcgcagg
     3481 atatgtattg ggaaaaacac ggagccttta cgggcgaaat atcagccccc atgctgacag
     3541 attgcggttg cagtcatgta atcgttggtc attccgagcg tcgtcacctg ctgggggaaa
     3601 cagatgacca cattaacctg aagatcaagg ctgcgttggc cggctccctg ataccgattc
     3661 tgtgtgttgg cgaaacattg gaggagcggc agaatgatca ggcctttcag gtggtacaag
     3721 aacagcttga ccatgctttg ttcgaagtgg atctagccac ccgcaatttg attgtcgctt
     3781 acgaaccggt ttgggcgatt ggtaccggcg tgaatgccgg ctcggaagat gcccaggata
     3841 tgatcgcttt catccgggga cagcttttcc ggcagtttaa ccaagaattg gcagacatta
     3901 cccccatcct ttatggaggt agtgtaaaag ctgccaacat gagggaattt atcgtaaaaa
     3961 aggatattga cggggcattg gttggcagtg ccagccttga tcccggcgag tttgcagaaa
     4021 tcgcgagggc tgtgaaaaat gtctaaagct ccgatggtgt taatgattct ggatggatgg
     4081 ggccataggg aagatcagca ggacaatgcc atcagtatgg ctcatcccca aaacttttac
     4141 aatttgtggg acgcttatcc ccataccttg ttggaatgct tcggcaatgc ggtgggactg
     4201 ccgcgagggc agatgggcaa ttccgaagtt ggccatctta atataggggc cggtcgtatc
     4261 gtatttcagg aaattacccg gattacccag gcaatcgaag accggtcatt tttcaccaat
     4321 ccggtttttt taaaggccat ggatgatgcc cgctgccaca acggagcggt gcatttgctg
     4381 gggctggttt ccgatggcgg cgttcacagt gatttgacgc acttgtttgc ccttttggaa
     4441 ttgtgtaaag accaggggat tgattcggtt tttgtccatg ctttcttgga tggccgggat
     4501 gtagctccgc aaagtgcaat ggagtatatc agcctgctgg agaatttcat gcggcagttg
     4561 gggctcggca ggattgccag tgttggcgga cgtttttatg ggatggaccg cgacaaccgc
     4621 tgggagagga ttgaaaaagc atatcaatca attgtactgg gggaaggcct acaggcggcc
     4681 aaagcccaag ctgccatcga acgcagctat gaggctcgtg tcagtgatga attcatggaa
     4741 ccggtggtta ttgttgatga acagggtcag ccaatcggca gaataagcga tggtgacagc
     4801 gtcatcttct ttaattttag agccgaccgg gctcgccaga taagccacag tttactggat
     4861 aaaaccatgg acagatttga acgcaaaatc gtgcctgcca tacattatgt ttgtatgacc
     4921 cagtatgatg ccggcctggc tgcgccggtg gcttttccgc cgcaaaacct tgacaataca
     4981 ctgggcgaag tgctttccgc tgccggcttg aaacagctgc gtattgcgga gactgagaaa
     5041 tatgcacatg tcacgttctt tttcaacggt ggagtggaac gtgccaatcc tggtgaagac
     5101 agggttctga ttccctctcc gcaagttgct acttacaatt tacagccgga aatgagtgct
     5161 tttgaggtga cccaaagggt cttgcaggaa atagaccgcg atttttatga tgtggtcatc
     5221 atgaattatg ccaatcctga tatggtaggc catactggtg ttctcgaagc agcggtgaaa
     5281 gcagtgaaaa caattgacga ttgtctgatt caggtgatca gcagggtcca ggagaaaaac
     5341 ggccaggtca tcattaccgc tgatcatggc aattgtgaaa tgatgacgga tgctgttaat
     5401 ggctgtccct ttacggcgca taccaccaac aaggtaccat ttatactggt tgatgaccgg
     5461 catcagggaa aaacactgcg agaaggttct ctgcgtgatg tagctccaac catgctgggt
     5521 ttgctgggct tgcccgcccc ggatgaaatg accggtaata ccttgctgag tatggaataa
     5581 accaagtgag tccagacaaa tatatggaag ggagataaaa atgagtatta tcgttgatgt
     5641 ttatgcacgt gaggtgctgg attcccgtgc caatccaacg gtcgaagtag aagttattct
     5701 cgaagacggg accatgggca gagctatcgt tccatcaggt gcttcaacag gtgcccatga
     5761 agcagtggaa ttaagggatg gagaccccaa acgctatggc ggcaaagggg tattaaaagc
     5821 agtggaaaac gtgaatgaaa ttatcgctcc ggagttgatt gggatggacg taacccacca
     5881 aattgacatc gatactttga tgattgagct ggatggtaca cccaataaga gtaaactggg
     5941 cgccaatgcc atgctgggtg tatctctggc ggcagcccgg gccgcggctg aatacctggg
     6001 cctgcccttg taccagtata tcggcggtac caatgccagg gagatcccgg taccgatgat
     6061 gaacattctg aatggcggaa aacatgcgga taataatgta gatattcagg aatttatggt
     6121 cgttccctgt ggtgctccta cttttgctga agctttgcgc atgggagttg aaatctatca
     6181 tagcttaaaa aaggtattgg gcagcaaagg actggccaca tcagttggtg atgaaggagg
     6241 ttttgctccc aatttagctt ccaatgaggc tgccatcgaa gtcattctag aggcaatcaa
     6301 tcaggctggt tatgtcgccg gacaggatgt ttttatcgcg cttgacccgg ctgccagtga
     6361 attctttaat gacggtcgct ataccctcaa tcaaaaaagc ctgagttctg aagaaatggt
     6421 ggatttttat gagggactgg tcaatcgata tccgatcatt tccattgaag acggactggc
     6481 ggaagatgat tgggatggat ggaaaatcat gacagacagg ttgggaagaa aaatccagct
     6541 gattggtgac gacctttttg tgaccaatac caaaaggcta gccatgggat tcgaaaaagg
     6601 agtctgtaac agtattctga tcaaattgaa ccagatcggg acactgaccg aaaccctgga
     6661 tacgattgaa atggcgagac gcagaggcta tacgacggtc atatcccacc gctcgggaga
     6721 aacggaagat tctaccatgg ctgatgtcgc ggttgccgtc aatgcaggac agattaaaac
     6781 cggtgctccg gcacggactg accgggcggc caaatacaat caactcttgc ggattgaaga
     6841 ggaattggat gagtttgcaa gatttaacgg gcgtaaagta ttttataacc tgaacaacta
     6901 gttgatgaac cttgtaataa attctgtgtt tgtggtaaaa tgtactgcag cattcgggaa
     6961 ggataaagaa aggaggttga atagtgctga aaatcattgt cctaatactt cagactttat
     7021 gcgcacttgg tttgattgcc acagttctgt tacaatctgg caaaagcgcg ggtttgtcag
     7081 gaagtattgc gggtggcgga gaggctatat tcggcaaaaa gaaaggcgtg gatgagttgt
     7141 ttgcaaaaat aactgcctac ctgtctgccc tgttttttgt cttgaccctg gctttggccg
     7201 ttatataacg gcaggtggat tgagccagga gaaatgactt gtttggcaat acctctgatc
     7261 ccatgattcc ggacgattgc tatctgcagt atcggacctg gaaatttatg ctgtagtacg
     7321 tttcgcagta aaactaatgt aatactacta aggagagaag tgatcaaaga catgaaattg
     7381 gaaacagtga agaaaacctg gatgactatt ttaagcagtt tggttatctt gctattaacc
     7441 ccagcaatgg ctttggccag cgaagctgac ctgattattc ctgaattaag cgcgtcccag
     7501 aacagcattc tggttgccgg aatagcggtt tgtgttttgg gtatgatttt tggtttctat
     7561 caattccaaa aggttaaaaa actacaagcc catcaggtaa tgcttgatgt tgctcaaacc
     7621 atttatgaaa cctgtaaaac gtatctgatc caacagggca aattcctggc gatattattt
     7681 gttttcatcg gtgtatgtat agccttttat ttcggatttc ttaaccaaat ggagattggg
     7741 aatgttttat tcatcctgat gtggaccatt attggtatcc tcggttccta cagtgtagcc
     7801 tggtacggta tccgcatgaa tacgctggcc aacagccggg ttgcttttac ttccctggaa
     7861 agaaaaccgc ttaagctttt gaacattgcc ttggatgcag gaatgagcat tggtgtttta
     7921 ctggtatgtg ttgagttaat tatgatgctt atcattctgc tttacgttcc gcgcgaactg
     7981 gctggtgcca gctttatcgg ttttgccatt ggtgaatctt tgggtgccag tgctctgcgt
     8041 atcgctggtg gtatattcac caagatcgct gatatcggtg ctgacctgat gaagatcgtt
     8101 ttcggtatca aagaggacga tccccgcaac cctggtgtta ttgctgactg taccggtgac
     8161 aatgccggcg acagcgtagg tccgactgcg gacggttttg aaacatatgg cgttacaggt
     8221 gttgcgatgg taacctttat tgttctagcc gttgcccctg cgctgcaaac aacacttttg
     8281 gcctggatct ttacgattcg tgtattaatg gttattacct ccgtactttc tttttatatc
     8341 aaccgtgcat atagccaggt caaatatgga agtgcagatg actttgactt tgaagcaccg
     8401 cttaccagtt tggtttggat tacttccgtc atttcaatta tcgtaacgtt cggagtcaca
     8461 tattatctgg tggggcctgg agcggccgaa gcaactgctt tgacaccttt gtgggtagta
     8521 cttgccgcca taattagttt aggtaccctg gcagcagcgc tgatccccga atttgtaaaa
     8581 gtatttacca gccccaaatc caagcatgtc gcggaagttg tcaaagcttc gcgtgaaggc
     8641 ggcccgtccc tcaatattct gtctggtctg gtagcgggta atttcagtgc gttctggatt
     8701 ggcatggtgt ttgtggcgat catgtttggt gcctatctgg ccagtcaacc catcccggcc
     8761 gagatcatga gttattcttc catttttgcg tttggtctgg tagctttcgg catgctgggc
     8821 atgggcccgg ttaccattgc tgttgacagt tatggaccgg ttaccgacaa tgcccagtca
     8881 atttatgagt tatctttgat cgaagaagtt cctaatgtag ccaagaacat tgaaaaggat
     8941 tttggcttta aacccgattt tgatatggga aaatattacc tggaagccaa tgacggcgcc
     9001 ggcaatacct tcaaagcaac ggccaaaccg gtgttgatcg gaaccgctgt ggtaggtgcc
     9061 accaccatga tattctcgct gattctggtt atcaaaaatg tactgggtgt tgatccggcc
     9121 actattctta ccctgctcaa tccttacacc attttaggat tcctgtgtgg aggttcagtc
     9181 atcttctggt ttaccggggc ttcaacccag gcagttacca ccggggccta ccgtgcagtt
     9241 gcctatatca agaaaaatat caaccttgaa gaggcggctg acgttaaagc caacacagaa
     9301 aaatccaaag aagtggtaca gatttgtacc cagtatgcgc aatcgggaat gtttaacatc
     9361 tttattgcca tcttctcttt tgccttggcc tttgcctgtt tgtctgcccc cagcaacggc
     9421 aacaatgaac cggtagcctt ctttgtggct tacttgattt ccattgctgt tgtgggttta
     9481 ttccaggctg tgtttatggc caatgccggc ggttgttggg ataatgccaa gaaagtcgtg
     9541 gaagttgacc tcaaggaaaa aggcacgatg ttgcatgaag caaccgttgt gggtgacact
     9601 gtaggtgacc ccttcaaaga cacttcttcc gtagccttga atccgatcat caagttcacc
     9661 actttgtttg gattgctggc aatggaaatt gcgatctccg aaacatttag gaatgtggct
     9721 ccttatgtgg gcgcagtatt cttgctgatc gccttattct tcgtatggcg ttcattctac
     9781 ggcatgagaa ttccggtcgg tgacgataaa taaagtagtt ataaaatgcc tctccatcta
     9841 ccagggtgga gaggcatttt tttatgcgtg gcgtaccccg gcaaggtttt ttcggcgaaa
     9901 tgatcgcttc atcatcgtaa atttgggtgc ttttttaaat aattacggtt ctttcctggt
     9961 aatcgacttg atttggtggt actcttcaat ccggtgggga gatgtttggt tcgcttttta
    10021 taaagaggat gaggattaac tgagcaagct gggggcagca gcgttcagga tttgttcata
    10081 tacatccaga taatcggcca ccatgcgctg acggctgaat ctctcttcga cccaaaggcg
    10141 acaatcaagg cgatttaagt tgctgatttc ctttattttt attactgcag aagcagtatc
    10201 gttgaccagg taaccggtta cgccgtccac aatcacctcc ggcatggaac ctttattaaa
    10261 ggcgatgatc ggagttccac aggcttgggc ttcgatgaca cttaagccaa aaggttcagc
    10321 aaaatgaatg ggatgcagca aagccaatgc cccgcccagc agttcattcc tatattttgg
    10381 gcccaccggg ccgacatagc ggatattagc ctcatccaga gcgggcgcca cctgcgtgag
    10441 gaaatatttt tgatcttgaa tgataccggc taaaatcaga ggcagcctgc tttttcgcgc
    10501 gatatcgatt gcttccgccg ttcctttgtc tggatggata cgcccgaagt acagcaaata
    10561 ctcgccagat tgcttctcaa aacgaaattg ctcaagatcc agaccgtgat agatattggc
    10621 ggtataatgt agatcgggat gacgatcggc gtaactgatc gatacataat ccgcagaagt
    10681 attatatttt ttatagaccg ggagaatttg cggcgaagaa aaaccatgaa tggtggtgag
    10741 caccggggta cttattaagc ggctgtaggt taagggcaaa aagtcatagt gattgtggat
    10801 aagatcaaag cgatcggctt gctcaaatag atgggcgata tgcaaacact cccagacttt
    10861 tggatccaaa ctacgatctt cttcataggc atgagggcag acccattcca ggttggcgga
    10921 ggctgaatcc ccggtggcaa atacgcttac tttgatgcct tcctgctgta atccttccgc
    10981 cagcagggag accatatttt cccagggacc gtaatgcaga ggtggagttt gccaggcaat
    11041 cggggaaagc agagcaattt tcattatcga ttaattcctt tcattggaga ggtaatttat
    11101 ttaagagaca ggctttcatt gatttaacca ttgttataat aagaaaatac tgttattgtc
    11161 atccggctta ctattttaat gtgccgcgta tctaaaatta tatactcgaa tatacaaatt
    11221 atgttctgat cggcaacacc atattctggt atgcaaaggt tggcagcctc agattcaacc
    11281 gattattact atagtaaaat gtctaaaatg caggtaaaat aaatatggct ttcgatacaa
    11341 caaccttcat tatattaata ttacaaagct tacattaaag gggggtattc attttggcga
    11401 atacatactt gcaagaatac cggcaaaaac tgattactgc cgagcaggct gcctcgctgg
    11461 tcaattccaa tgacagaatc ctctacgcag tatttgccgg acggccgaca gattttgata
    11521 ttgctttgag ccaacgcaaa gaggaacttt tcaatgtaca gatcactcaa tgcggaggca
    11581 acaatattgg tcctgacagc tctgcttttt ctgatccaag ccgtcagcac ttcaccaaca
    11641 acagctggtt tttcgatggc gttgatcggc agttgcatga tcaggacatg atgtattatc
    11701 atccctgtca gtttggccag ctgcaggaca tcatcggcag cgatctattc ccctatgata
    11761 tctatgtgca aaaggtaagc cccatggacc atcacggtta tttttccttt ggtttgtcca
    11821 atgttaacag cctggagtct tgtttgggtg ccaaaaaagt cattctggaa gtgaatgaaa
    11881 acatgccccg ggtgccaggt ggttcagaag atgccgtgca tatctccatg gtcgatcata
    11941 ttatcgagag cgccaatgct ccattattgg tactgccccc cagtcgtgag ccttctccgg
    12001 ccgatgtaac catggcccgg ctgttgttgc cggagattcc tgatcgggct tgtctgcaac
    12061 tgggaattgg ggcattaccc aatcttttgg gggatttgtt atgtgattct gatttacagg
    12121 atttgggaat tcatacggaa atgtttgttg attccatggt gaatttgttt tccagcggca
    12181 aagtgaccaa tcgttacaag acgactgacc gctgcaagat cgcttttact ttttccatcg
    12241 gctccagaag tacgtatgat tttatggaaa acaatccgct catggccagc cattgtggca
    12301 gatacaccaa caatgcccgt ctgatagcga ccaatgacaa ttttatttcc atcaacaata
    12361 cggttatgat ggatcttttt agtcaggctt gttctgaaag ttcgggtacg cgtcagattt
    12421 ctggtactgg tggtcagatt gatttcatca acggttcctg gttctcacgg ggaggaaaga
    12481 gttttctctg cctgaccagc acctaccggg ataaaaatgg tcagctccaa tcacgcatcg
    12541 tacccacgtt gccctccggg agcattgtca ccacagctcg cacatttata gattatgctg
    12601 tcactgaatt cggcatcgtc aagcttaaag ggaaaccaac ctggcatcgg gctgaacaat
    12661 tgatcagtat tgcccaccct caatttcatg acgatctggt aaaagaagca cagaagatga
    12721 aaatttggcg gcgcagcaat aaaattgagt aagggccttg actgacggga cggcgggaaa
    12781 ggccagacag ggtaaatgta ttagggcatt acctgcgata tgatgtaacc aaaccacaac
    12841 aacgcggcaa cgtttagagc gcttctttat gaaggaggaa atgatttgta ttctcagact
    12901 tttgcaaaca tgggactcaa accgcgtttg ctgcagatga tcgaaaacaa gggattcgaa
    12961 attcctactc ccattcaaat taaagcaatt ccgccggctt tggcaggtca tgatgtaatg
    13021 gggcaggccc agacgggcac gggcaagacc gcggcttttg gtattcccat gctgaatcga
    13081 atggtccccg gacggggact gcagggtctg gtcgtttgcc cgactcgcga attggctgtc
    13141 caggtaaccg aagagatcaa ttccctgggt aaaggaatga acctgcggtc cttggctgtt
    13201 tacggaggac agtccattga actgcagatc agagcattga gccgcagacc ggaattgctc
    13261 gtggggactc cgggaagatt attggaccat atgcagcgcg gtaatataaa cttgtcaaat
    13321 ctatcttatc tggttttgga cgaagctgat gaaatgctgg atatgggttt tttgccggat
    13381 atcgaaagaa ttatggaagc ctgttcacat gaccggcaaa cgttcttgtt ttcggctacc
    13441 ctgaacgagg agattcgcag attgggcagc caatttatgc atgatcctga tatcatattg
    13501 attgaatcac tggaattgac ggtgcctttg acccggcaat tttatttgga agtaagtccc
    13561 cgttacaaga tcgcaaccct ctgtcggttg ctggaagttg agcaacctac cgttagtctg
    13621 gtcttctgcc ggaccaaaaa gggggttgat atgctggccc atgccttgaa cgaccggggc
    13681 tatgatgccg atgccctgca tggcgacatg acccagcggg aaagggacaa tgtcatggaa
    13741 cgtttccgca agggcaatgt aaccatattg gcagctactg atctggcggc acggggcctg
    13801 gatattgaaa tggtcagtca tgtttttaat ttcgatcttc cggaagatcc ggacagttat
    13861 gtgcaccgaa ttggcaggac cggtcgggca gggcgcgacg gtattgccat atcttttgtt
    13921 gaaccccggc aaatgcaaca aatcaaattg attgaacacc acatcggcaa gaagatcatg
    13981 cgccgccatc caccttcctc gagagatgcc gtggagagca ggcgggaaag cctgaatcgg
    14041 cgcttgctgg aaacagcccg tgaggggggg ttggaggggt atcagcagat ggccggcact
    14101 ctgctgacgg aaaatgatgc ccagaccctg ctggcagcgg cgcttaaaat tattgcgggc
    14161 ggagaaccgg aaatggaatt ggcggaaagg aaaacgaaca gtttggatac ggcacatgtt
    14221 gaaatgccct tgggacgcat gcaggggatg aatccccggc gattggcaga ttatttgtgt
    14281 gccaatacta cgctgacccc cagacaggta ggggacatag aaattcacag caataccagt
    14341 tatgttgaag tgccgatgga taaaattgat gaggcatatg aagcgttggc cggttatgag
    14401 cgaaatcgta aaagcggaca cccgcgattt ggtttgtccc gttccggccg caataaaaat
    14461 ataaactaga agccgggggc gataactcgc ccccggcttc atatagcagt cattttctat
    14521 aaggttgatg actaaccaag ctgtgatttg accgtttgaa tatcagactg gtcagcttgg
    14581 gcagcggctt tgtaaaatcc cttttgttct gccatctgat aaagctgata ctggaattgt
    14641 tcatccgaat ttctgatttg ctgaatggtc tggcgcagct gctggttgga tgtttctccg
    14701 attacctggg cataagtggc cagactgctt tttaccgaag acaaaatatc gttgaccatt
    14761 tctttatcct gtatttgttg tggcatagaa attttacact cctttactgc aagaatgata
    14821 tcagtttgtt gcgggtattc attgcggatt gtgccgcctg atcaaacatc tgcttaatct
    14881 gggcatcctg acattgagaa gcataaaagc gaagtttctt ctcggcggtg gtgtgccccc
    14941 cgatcaaatg tctgaggttt tgaagctcca tttggttaac cgtcacgttg tttactcctt
    15001 tctgtataag ttgttttccc cttttaggat gacccaaatt aaaaatatta tgcttcccag
    15061 aaagcaaaaa gacggtttcc attttgacca ttttctcctg cgcagttgac cggactgata
    15121 cttgtctttt tgcacagcag gcgtttgagt ggaaacagtc ttgttcttta ttgtctgaag
    15181 aggatttaac caatcattat ttttccctgg gcataatgga tttccggctg ttttttcttg
    15241 taagccaggg caaaacccaa ggtaagcggg ccgacccggc ccaggaacat acatatcacg
    15301 attaagattt tcccaaatgc attgagttcc aagcataacc ctgtagaaaa tccgactgta
    15361 cccaaggcgg acgttacatc aaacaaaatt ttcataaaat ctcccggatg agtcagggaa
    15421 atcaggaaga ccatgatgat caacatgaaa attgccataa aaaacaaagt aaacgatgga
    15481 tatacgtcgc tgctgacaat ccggcgttca aaaatttcga tatctttctt gccccgcagt
    15541 tgggctacaa cgactgcaaa catcagggct accgtggtcg tcttgattcc gcccccggtc
    15601 gaacccggcg aagcgccgat aaacataaaa acgatcaaga gcagctgact gggcagcagc
    15661 agagaattga tatcaatagt ggcaaacccg gacgaacggg aagaaatact ttgaaagaag
    15721 gcaaccataa gtttatgtcc aggtgtcaag gcttttaaag catggttata ttcgactgcc
    15781 agcaataaaa aggtgcccag caccagcaaa ataccggtgg tggttaacac gactttgctg
    15841 tgcagggaaa gcttgcgtga ttggcggtaa ttatacagtt cgtaacagac gaaaaaccct
    15901 aatccaccga tcatgaccaa cagactaaag acaatattta tggttatatc atgactaaag
    15961 tcggtcaggc ttttaaaatt gccaaacaga tccaggccgg cattgttgaa tgctgaaata
    16021 gagtggaata ccccatgcca cagcgccttt ttaacgccca tcagcggcat ccacttgatg
    16081 gccagaatca ctgctcccag ggcttcgagc aggaaggtaa accccagcag atatctgaaa
    16141 acccgtacga ttcctcccac tgcagaaaca ttaatggatc gttgcatcag taatcgctcc
    16201 cgcagctgga tctttcttcc cattaacatg gcaaagaagc cggcgaaggc cataactccc
    16261 agcccgccaa cctgaaccag cagcaggatg atgatctgtc cgaagagggt ccaatgggtg
    16321 ccggtgtcga caaccaccag accggttatg cataccgaag tggccgaggt gaacaaagca
    16381 tccacataac gagtgggggt gttggcggcc caaggcatat acagcagcag gctgcccaac
    16441 aggatcaaaa cgagaaaact gcctaccaac aactgggggg cggacatctt gttaatttgc
    16501 atagcattga cctcgaaatg gtttagtcca ggttagtgat atattccaga tcatcattgc
    16561 tgcccagggc aacaatcaca tcgcctttgc gtagtctttc ttccggaccg ggtgctacaa
    16621 taacatcgtc gttccggcgg attgccagta ccgtaacgcc catttttttg cgcagaccag
    16681 tttccaccag actcttatct gccagcgtaa ccggcgtaaa gaattccatc acgctgtggg
    16741 tgggagaaag attgatttgc tccagaaaat tgttggaaac cagggagcgt gccagtttta
    16801 tggccatatc ccgttcgggg tagatgacca gatccgcccc gatcttctcc agaaccttgc
    16861 cgtgcaaagc gttttgggct ttggctacaa ctttattgac ccccaattct ttgagcatga
    16921 tggtggtcag aatattggat tgaatgttat tgccgatagc tacgatcacc acgtcaaaat
    16981 tcctgattcc caaagctttg agcgtatctt catccatgga gtcggcctgc actgcatgcg
    17041 ttgcaatatc cacagtatca ttgatttttt cttcgtcaat atcaatcgcc atgacttcat
    17101 aacccagtcg ggtcaattct ttgaccaggc tttcgccaaa tctgccaatg ccgatcactg
    17161 caaattgttt gtttttcatt gccatacacc cttttttcat gagtatttgc tgttggatat
    17221 cgtatctatt tccttatcat tacgtttatc gaaccgatta aaatgagttt aatacttgcc
    17281 aggaaacatt gcaatcaaaa gacaaaaata cccgactaaa cctagccggg tccaaaaatg
    17341 attgatgaaa ccatgcgaca aaaagcatgt ttagagcaat aacaggaacc ctaccatgga
    17401 aaaataaagg atgcctgtac aaaaaacaga ggcacagaaa gtctgaataa actccttctt
    17461 gctgtaatcg gcgaacatgt gatcaccccc tggattagaa tatttgttct gattccattg
    17521 taaataggaa cgattgttct gtcaattgtt ttttaaaata aaagattcga aaagattgtt
    17581 gccatcccca aaaattatgt aattggaatt attcacaaag gaactgttta tccacccacg
    17641 ctttttggta aaatgggttt agacataatt aagcgtaagg aggatggggc gtgtcgtacg
    17701 agtatcaaat tgttgacaac atcgttattt ttcaatttaa ccgcggaaaa ctgaattcga
    17761 ttaccatgga aacattgcag ggtttgaatg aagtcattga tcgtgtcaat caggaaaatg
    17821 aactgaaagg gattatcctg accggtcagg ggagatactt ttcaggcggc tttgatctgg
    17881 gcacctttac cagtttccaa agcaaggaag aaattctgga ctggtttaat tacgaggaag
    17941 aggttttatt taaactattc acctgctcca aaccggtagt agccgccatc aacggccatg
    18001 ccaccgccat gggaatgatt ctttccatgg cctgtgatta ccggctggtc atcaataatc
    18061 ccaaaattaa acttggcatg acggagatca aaatcggtct ttccctgact ttggtgcagg
    18121 gagcggtgat gcgttttggt ctggatacgg ataaaaaata ccgcgatgtg atctacaagg
    18181 gtgagctttt tgcaccggat tatgctgtgg cccagggaat ttttgatgaa ttggcagaga
    18241 atgaagaaga acttttggag aaagccaagg ccaaagtggt gagcatgatt gataccccgg
    18301 gacgtccttt cattatcctg aaggaactcg aaaaaatgca gaccgtcgac tggctaaagg
    18361 gaaaactgaa ggaagtggac tgggggccgt tggtcgcgac ctttaccgat gaaaaagtgg
    18421 tcaacacttt aaaaatggtc aaggccgcac tgggtgaata aaaatactga aatgaggtgt
    18481 cttacatgag tttggatcat ataaacgcac agcaggcgct gcaacgactc aaag
//
LOCUS       DAOTHZ010000042        18118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3716_length_18118_cov_7.456070, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000042 DAOTHZ010000000
VERSION     DAOTHZ010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 18118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18118
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3716_length_18118_cov_7.456070"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1378
                     /gene="icd"
                     /locus_tag="P5549_06370"
     CDS             95..1378
                     /gene="icd"
                     /locus_tag="P5549_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRW12733.1"
                     /translation="MTEKTSLGDKITVTNGILNVPDHPVIPFIIGDGTGPDIWAAASR
                     VLDAAVDKAYEGQKSIVWKEVLAGGKAFDLTGEWLPQETLDIIKEYIIAIKGPLTTPI
                     GGGIRSLNVALRQKLDLYTCLRPVRWFAGVPSPVKRPQDVDMVIFRENTEDIYAGIEY
                     PQGSPEGEKLLHFLLDEMGVDKIRFPQTSALGIKPVSEEGSKRLVRAAIEYALAKGCK
                     SVTLVHKGNIMKFTEGGFKQWGYDVAEAEYGEKVFTWNQYDAIKDKDGAATADKAQKE
                     AEAAGKIIIKDAIADIFLQQILTRPAEFDVVATLNLNGDYISDALAAQVGGIGIAPGA
                     NINYQTGHAIFEATHGTAPKYANLDKVNPSSVILSGEMMLRHLQWDEAADLIIKAMEK
                     TIASQVVTYDFARLMEGATEVKCSQFADALIQNMS"
     gene            1469..3004
                     /locus_tag="P5549_06375"
     CDS             1469..3004
                     /locus_tag="P5549_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12734.1"
                     /translation="MNSNQFKEALQDKNRLAVTWELVPGRGAREKSQENAIRAAEQAA
                     RGGRIDALSITDNPGGRPALSAACLGMEIVKLGIEPLVHFTCKDKNRNQIESELYALN
                     RAGVNNLLIMTGDYPVSGFKGRPKPTFDLDSVQVMELVSRMNQGLEVNEAGKTVFHQP
                     GTFFAGSVVSPFKASEAELMGQYQKLRKKISQGAGFIITQLGFDARKFHEVLQFVKNI
                     DNDLPVIGNLYILPYGAARMMNANQLPGCVVPDKLLAQLDQERNAEDKGVGARLLRAA
                     KMYALLKGLGFDGVDIGGHNIKYEQVEYIIDQGEELSADWDKLLSEFDYPQPQGFYFY
                     ARDTKTGLNTPDAVDRSDLPAEVDIGLNYRMMRMFHELFFTPDKNFYPLLYRYYARVK
                     DPRRYKLEHVLKVIGNDCRDCGDCALLDLAYLCPMSQCPKNQRNGPCGGSYQGWCEVF
                     PNEKKCVWVRVYALLKAYQEEDRLENQYLPPVNWDLYQTSAWYNYFAGRDHTGGNKKE
                     PAL"
     gene            complement(3087..3917)
                     /locus_tag="P5549_06380"
     CDS             complement(3087..3917)
                     /locus_tag="P5549_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRW12735.1"
                     /translation="MTTVLITDSTAYLPVFIIRQYDINVVALNVHLPDRDFKEGYGFS
                     NQEYYQYMRNNPVFPTTSQPSAGEFLELFQKLGPDDEALVILISSKLSGTVQSAQIAV
                     SMLPEPHPMITVIDSLSGAMGLGFQVIRAAEMLAAGYRPHEIKPVIEAMRQSMQIYFM
                     VNDLEYLVRGGRISKLSGILGNVLQLKPILNVKDGEIVLFDKVRTAGKALQRILAEME
                     THRPTLHKICVLHVDALESAQQLQGKLQEEYALPVYLVEAGPVIGTHVGPGCLALIYY
                     "
     gene            4095..4793
                     /locus_tag="P5549_06385"
     CDS             4095..4793
                     /locus_tag="P5549_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12736.1"
                     /translation="MSGPRVVRIPLRFANSYLLIDQKTIIVDSGDPGFSSGILKAMQE
                     NGVKKADVSMIFLTHGHIDHFGSTYELKKHLDVPVAIHTSDLLYFLNGTQAPLHPTNA
                     LAAMIKVIGKNMQVKKRYGIKAEIVFDEELKLHEYGVDALAVATPGHTLGSASVVLPD
                     GSAVVGDLLVRRNFLFGQACIPPFLHDGQKHAESMRKLYDMGVKKFYPGHGKVLDLEE
                     LNWRLQPQHYTLQD"
     gene            complement(4790..6031)
                     /locus_tag="P5549_06390"
     CDS             complement(4790..6031)
                     /locus_tag="P5549_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRW12737.1"
                     /translation="MIEKKMKNTAARSCKLVIIMVILTWLNYFYGQLHAACFFDHPVI
                     FPMVSSLVALLLVSLLYFQHRNISQSLNKLLENERASANWRFTKSPLFDNIGHELRTP
                     IIGIMGSSDLLAQSVLDVEQKAHLETIKTCGEKLLTSIDVLLSIAKMNEHHNHNMVTS
                     TDQSLDSKQGTGCINEENHAYLFNQFLPVNILLVEDNELNQKLITQMLVSYGFEVVSV
                     SNGLECLQVLQEKEFHLVLMDMQMPVLDGYETTRKIRANSLYKHIPVVAITANTLRYD
                     IEKCMDCGCCSYLPKPFTADELAQEIKSHLKIDPPGSSSQPADDLISQLLPEFIEILA
                     EMLTDLHKAVDQHDMTAIQELSHAVKGTAGMYGFMQISESAALIEQAGKGKVYSSIPL
                     LINQLDKYYEQIIRQKTPIVG"
     gene            6418..7764
                     /locus_tag="P5549_06395"
     CDS             6418..7764
                     /locus_tag="P5549_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HRW12738.1"
                     /translation="MYKLRLAKRMMMLGLCLLLVGAAVKPVGAVPVSGSSDFSIYMNN
                     EQLVIPADDQQPVIIDGRTYVPLRVISENMGAKVEWIEAERQVIILWKKTSIDIWPDS
                     RGEDVQIIIDGKILEIPEDLGKAYISERGRTMIPLRAVGEAMDCAVDWIDASRTVEIH
                     YERPVSEATEPIKETPPEPTDASDQDNLQMFKDLAAFRTNLKMLDGKVMNSEELLTRQ
                     PTDFSAEQLAVLQTYLSQLSKYQALITLPDGSAVNSAEVSILGDSYLTARQLTAWIEQ
                     ETPRLMAKAAANGVEFKPIPDLARLYIDIGKEYGIRGDIAFCQAAKETGYWQFTGSVT
                     PEQNNYCGLWATGSPLTGQESLNGADPEQVTFVAGLHGATFATPAVGVEAHIQHLYAY
                     ATKNPLPKGKVLVDPRFALVSKGVAPTWLQLNARWAVPGTTYGQSILADYWLIAAK"
     gene            complement(7863..8537)
                     /locus_tag="P5549_06400"
     CDS             complement(7863..8537)
                     /locus_tag="P5549_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HRW12739.1"
                     /translation="MSKSNTNEKAGPISEVRVSGHVQDLVANPSPLGLLGLAMVTLVA
                     STQKLGVTDGVAYIIPWAIFLGAAAQFVAGLMDYKHDNTFGATAFCGYGFFWFAVGMT
                     WYMKVTGTIGEGAGFDPHQLGYAFLGYFIFTAFMTVGAMGANKVLFIIFFLIDLLFLG
                     LFMSTLGWGGEAWHMLAAFSELGIAIMSFYGSAATVLNTHYGFTVLPVGAPFGSLAKK
                     AAALKK"
     gene            8971..10848
                     /locus_tag="P5549_06405"
     CDS             8971..10848
                     /locus_tag="P5549_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12740.1"
                     /translation="MTANFAYKNTRDLKFILKEWLPTEEVFAYPRFADYYSKEDVDSV
                     LDTCLKMSKELIEPSNEDGNANPCRYDNGKVVTPASFGPLYKMLQSEGWGTSNIDEAE
                     DAMTLPTILRFAVWELFLGANPAFLPYIMLTTGAAELIQSFAAPDLKEIFLPKMMDGT
                     WAGTMCLTESTAGSDVGDILSKAYPTDDPRIFKIKGSKIFITGGDNDFTDNIIHLYLA
                     KVEGSVKGTKGISLFIVPKYWVNEDGSLSDNDVQTTGIEHKLGQYGSVTASLSMGENG
                     NCRGWLLGKNPLENDGVGEGMAQMFEMMNGARMETGLAALACTANAFYNARDYCQERI
                     QGRLMSDPRGDRVSIIRHEDIKRTLLMGKAHIEAMRAMMYKAFLAFDVRFCDPDPEQR
                     KKANALVEISTPLCKAYPSDEAWWLIGEAIQAYGGYGYCEEYPVAQIARDVKIYSIWE
                     GTNFIQAMDLVGRKWTMGKGAAFAAFMQEMRDFCAASKPSAEAAATGEAGAMDLSLNK
                     EFAILEKALDAYGQMQMAIGGYFSKKEFSMMPLYARRILTATSQLYAGRCILDQALLA
                     AKKAQQVGPDHYDYPFYIGKILSAKYYVRNVVPNVWTVADIVKDGDKSALDIPLESFA
                     Y"
     gene            complement(10918..11154)
                     /locus_tag="P5549_06410"
     CDS             complement(10918..11154)
                     /locus_tag="P5549_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12741.1"
                     /translation="MNDQYPRQSQPSAGENQFHASLLDSLRDINELLVHHGSPQLHQD
                     ITAFLAEVDGNSGATEDLLPAIRYKLYALLALSR"
     gene            complement(11158..>11765)
                     /locus_tag="P5549_06415"
     CDS             complement(11158..>11765)
                     /locus_tag="P5549_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12742.1"
                     /translation="ASSLKEVIDLLGKAQKNLQVLIKQHGKSANPEFSIFLEAGENFL
                     HDTWPAFKNISKSNPMDYSQLNNMLAQMRNQFGWLETQLDHLANQAEHIQKTQQQYAQ
                     IQDTLDNYQKMLTEMKNQLARTLAPRNLSRQFKDLEVRVEHIQLTQEESFPDAYLALL
                     DHTPGQAGQQLIIEEEDGDLFLFRVGDIQDVLVPHIRLREG"
     assembly_gap    11766..11865
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11866..12878)
                     /locus_tag="P5549_06420"
     CDS             complement(<11866..12878)
                     /locus_tag="P5549_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12743.1"
                     /translation="MHLPEPEVRENMLELSSFLRQIKDELQIQSQCLPGVDVNTVDEF
                     ILDLNNYYYQRFHEQFIPLYRAQWEAEIKRLYGIARDESALREDIIKNLKRSAQIYNQ
                     VKNALHLLKDNQEQINHLHFALERNQAQLLLELIYALKETETVLAACLDLLHTGQTLS
                     TEKELIKSMKVYPFQSILKNLWNSWDPDSSTWGRDYKRLLINLELFAALVGKISGNDD
                     KRADDWAMWLAELNKTEHQLNNKKYSPALHEWYRQDLRPQLMIYTELLALNIKLRDRK
                     GLQAAVHHFESWLQALLFLLERSNRRNGSPEPLLLEMNYAHSADASSLKEVIDLLGKA
                     QKNL"
     gene            complement(12879..14330)
                     /locus_tag="P5549_06425"
     CDS             complement(12879..14330)
                     /locus_tag="P5549_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12744.1"
                     /translation="MRNILQRWKELPRDDLFTFWQEEVQLRKELRQARCEYRELAIES
                     KEQYDFLRLGLAYGGHRTAFLQILFYNMDSQTVQNQLLQGPLPLYQAFLRFLPRIITA
                     EKPSSQRLQFLISLYREDFAQEYQEILANLEEEHCKYLLDRTANQSLRTMLKDKNAQH
                     HQQRTQSHYGLLYRPTPSCPTLFGDKTALLKQTVQRLATPPIDHRENLFLALGYDLES
                     AEQLFMLGMLEECLALQHFVYQQWTTGRSELSVEDDALLFKAISRNLAKSLSLYALIS
                     NAMPYRYSASLYHRYYPEIPADKNAQAYLHLYQSLQTSHDSNPEYTMIELAHICRQHG
                     LNEVIQWVSSLIDHAVADEPTVASLLAEIDRDLTAMPHRALTNMRLLLFGARHRKIVP
                     TRSWAGKLLQNHLSLFQWFPAASFLNQSIYEQLSASVEDEQQKEADRLWLIASQYTRG
                     SIRELYQQQTERFKNENNPILQQLLLGSFLGVK"
     gene            complement(14347..15069)
                     /locus_tag="P5549_06430"
     CDS             complement(14347..15069)
                     /locus_tag="P5549_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12745.1"
                     /translation="MGIAANAPETINIITDKLARHIRLDEAELRQLLAYARRLRRKSF
                     ENYRSFYDSYEQVLYREYGHCLPLFRYGRDDFYDYLVQNPDFLAKHPQALLGLEEFPP
                     FLHDYLHYSYGSTIDPLAITSLLELLVGRVQPDYGLPAPRQREVVYKYEKGNSYKELG
                     LKSHFERIGRYDFVSRVQSYRYLRGNKSSTDKIEVLGPDFLGGIFTNKEKSIYYYIFL
                     TENHYQKAVNACQLLNNDLYGK"
     gene            complement(15109..16023)
                     /locus_tag="P5549_06435"
     CDS             complement(15109..16023)
                     /locus_tag="P5549_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRW12746.1"
                     /translation="MKKTGLILLMAVMLVWLSGCVPPDSDEQAVKVPITNDVPAPEAN
                     PANPVVPEASTTVPDKAPTAPAEGTAHEQADSTTSPTLSWYFIRNDQHQMPGTNAEYK
                     ELLAQHQAFYILPNDRQNIYLTFDCGYELGYTSQILDTLAAQNVKAAFFITGQYIKTQ
                     PDLVRRMHEEGHLVCNHSFNHPDFSTISQSKLEQEITSLESLYRDLTGQDMDKYLRPP
                     MGNFNANSLQWTKALGYSSVFWSMAWKDWDPQQQPGADYVYQHVIDNIHPGAVILMHA
                     VSESDTQALEKTITTLLSQGYVFSTFGS"
     gene            16214..16822
                     /locus_tag="P5549_06440"
     CDS             16214..16822
                     /locus_tag="P5549_06440"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HRW12747.1"
                     /translation="MNERFRITIFPVMGLLLLLLVIGQNWPLLTENPNSVVYTYFHSF
                     VSGEIGSGYGQAAAGEIPFHLLEPGDILLGGWPNCAYGKYSHAGLYLGNGEVLEAYVD
                     YGVCVQPLDHYIQYSYLSLVKVNVSEEIKAAAIKAGRSYIGQTFYPVAFKHNDRYYNC
                     SSIIWKAYQEQGINLDINNDIWVAPDRFGDNPYVKVLFTKEM"
     gene            16841..17263
                     /locus_tag="P5549_06445"
     CDS             16841..17263
                     /locus_tag="P5549_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12748.1"
                     /translation="MRLYTIQLAAILAVWLGMYYPGLDIILAGLFLLVVWGEADYQRF
                     SWLQQSLIAIIWQLPALFLSLSVLLGLDKATDFSYYFIFMLQLWHTPLLPLTTVLPDL
                     LQLDKPFYYYELFVMTPLLWMVYFLPRLKRLLPGRPIN"
     gene            17626..>18118
                     /locus_tag="P5549_06450"
     CDS             17626..>18118
                     /locus_tag="P5549_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HRW12749.1"
                     /translation="MIFQTKLDQEGQIGPIKVKNRIVMPAMATGLGSINGEVTPAMIQ
                     YYQERAAGGAGLIIVEIACVDSPQGRGSLTQICADHPRYVFGLNQLCEAIQSYGCKAM
                     LQLHHAGRQTSPFATDGMQPVAPSAIACKLMRAMPRELTLDEIAALKNKFVSAAFFAS
                     QAGF"
BASE COUNT         4732 a         4046 c         4583 g         4657 t
ORIGIN      
        1 cggactggtg gagcacttta aaaaatatgg cggattcaat tttgattaaa gcgatgctgc
       61 aaggcatggc ggataaaaaa caggagggct gaccgtgaca gaaaaaacaa gcttgggaga
      121 taaaataacc gtaaccaatg gaatcctaaa cgtgccggat catccggtta tcccttttat
      181 tatcggcgat ggaaccggac cggatatttg ggcggcggct tcccgggtgt tggatgcggc
      241 ggtcgacaaa gcctatgaag gacaaaaatc aatcgtctgg aaagaagtgc tggccggggg
      301 aaaggccttc gacctgaccg gtgaatggct gccccaggaa acgctggata ttataaaaga
      361 atatatcatt gccatcaaag gtccgctgac cacaccgatt ggcggaggga ttcgttcatt
      421 gaatgtggct ttacggcaaa agctggattt gtatacctgc ctgcgtccgg tacgctggtt
      481 tgcaggggta ccttcaccag tcaaaagacc gcaggatgtg gatatggtga tcttccgtga
      541 gaatacggaa gatatttatg ccggcatcga atacccgcag ggcagtcctg aaggagaaaa
      601 actgctgcat tttctgttgg atgagatggg ggttgacaaa atccgttttc cgcagacttc
      661 tgcgctgggg atcaagccag tttcggaaga aggcagtaaa cgactggtgc gggctgcgat
      721 tgaatatgcg ctggccaaag gctgcaaaag tgttaccctg gtacacaaag gcaatattat
      781 gaaatttacc gagggtggct tcaagcagtg gggttatgac gtagctgaag ccgaatatgg
      841 tgagaaggtt tttacctgga atcaatacga tgccattaag gataaagatg gtgctgccac
      901 cgccgacaag gcccagaagg aagcggaagc cgccggcaag atcatcatca aggatgcgat
      961 tgccgacatc ttcctgcaac agatacttac ccgtccggca gagtttgacg tcgtagccac
     1021 gttgaatttg aacggtgatt acatttcgga tgcgctggca gcacaagtgg gtggcattgg
     1081 tattgccccc ggtgccaata tcaattatca gaccggacat gccatttttg aagccaccca
     1141 tggcactgcc cccaaatatg ccaacctgga caaagtgaat ccttcttcag tcattctttc
     1201 gggagagatg atgctaagac acctgcaatg ggatgaagct gcggatctta tcataaaagc
     1261 catggaaaaa accatcgcca gccaagtcgt aacgtatgat tttgccagat tgatggaggg
     1321 cgcaaccgaa gtgaaatgct cacagtttgc ggacgccttg atccaaaaca tgtcctgaga
     1381 cgattttccc atatgcagat aaagacaagt cagcggtcgg gtcagcccga ccgtatcttt
     1441 tcacgagaac gagccgggag gagaggccat gaacagcaat caatttaaag aagccctgca
     1501 ggataaaaat cgtttggcgg tgacatggga actggtaccc ggtcggggag cccgtgaaaa
     1561 atcgcaggaa aacgctattc gggcggctga acaggctgcc cggggcggca ggattgatgc
     1621 cttgtccatc actgacaatc ccggcggcag accggccctg tcagcggctt gcttggggat
     1681 ggagattgtg aagctgggca ttgaaccgct ggttcacttt acctgcaaag ataaaaatcg
     1741 caatcagatc gaatctgaac tgtacgctct gaaccgggcc ggggtgaaca atctgttgat
     1801 catgaccggc gactatccgg tcagcggatt caaaggccgg cccaaaccga catttgatct
     1861 ggattcggtg caggtaatgg aacttgtttc tcgcatgaat caagggctgg aggtcaatga
     1921 ggccggcaaa acggtttttc atcagccggg cacttttttt gccggcagtg tggtttcccc
     1981 tttcaaggct agcgaggccg aactcatggg ccaatatcaa aaactgcgca agaaaatcag
     2041 tcagggggca ggatttatta tcacccagct gggttttgat gcccgtaaat ttcatgaggt
     2101 attgcagttt gttaaaaaca ttgacaatga tttgccggtc atcggcaacc tttacatcct
     2161 gccctatggg gcagcgcgga tgatgaatgc caatcaactg ccgggctgtg ttgtcccgga
     2221 caaactcctg gcacaactgg atcaggaaag aaatgctgag gacaaaggcg ttggagccag
     2281 actgctgcgg gcagccaaaa tgtatgcttt attaaaaggc ctgggctttg acggtgtgga
     2341 catcggcggg cacaatatca agtatgaaca ggttgaatat attattgacc aaggggaaga
     2401 acttagtgcc gattgggaca agttgctgtc ggagtttgat tatccccagc cgcagggttt
     2461 ttatttctat gccagggata caaagacggg tttgaatacc ccggatgcag tcgaccgcag
     2521 tgatctgccg gcggaggttg acatcgggct taattaccgg atgatgcgca tgttccatga
     2581 gctgtttttt acacctgaca aaaattttta tccgttgctg taccgttatt acgccagggt
     2641 caaggatccg cgccgttaca agctggaaca tgtgctcaag gtgatcggca atgactgccg
     2701 ggattgcggc gattgtgcct tgcttgacct ggcctacctc tgccccatgt cgcagtgtcc
     2761 caaaaatcag cgcaacggtc cctgcggcgg cagttaccag ggctggtgcg aggtctttcc
     2821 caacgaaaag aaatgtgtct gggtgagggt ctatgctctg ttgaaagcct atcaggaaga
     2881 agaccggttg gagaatcaat atctgcctcc agtcaactgg gacttgtatc agacttctgc
     2941 ctggtacaac tattttgccg gccgggatca cacaggtggc aataagaaag aaccggcgct
     3001 gtagaaaaac gccggtcatg gtgcagagca ttgcctagtg aacttgacac gagggctgtt
     3061 tgaacagaaa aattcaaaca gcctttttaa tagtagatta gcgccagaca tcccgggccc
     3121 acatgggtgc cgatgacggg accggcttcc acaaggtaga cgggaagagc gtattcttct
     3181 tgcagtttgc cttgcagctg ctgggcgctt tccaaggcat cgacgtgtaa tacacagatc
     3241 ttgtgcagcg ttggccgatg tgtctccatt tcagctaaga ttcgctgcag cgccttgccg
     3301 gctgtacgaa ctttgtcaaa caagacaatt tccccgtctt tgacattcag aatcggttta
     3361 agctgcagaa cattgcctaa aataccgctt aatttgctga tcctgccgcc ccgcaccaga
     3421 tattccagat cgttgaccat aaaataaatc tgcatgctct ggcgcatggc ttcaattacc
     3481 ggtttgattt catgggggcg atagcctgca gccagcattt cagcagcccg gatgacttga
     3541 aaacccaatc ccatagctcc tgataatgaa tcaatgacag taatcatcgg atgaggttcg
     3601 ggcaacatgg aaaccgctat ctgggctgat tgcaccgtgc ctgacagctt ggaagagatc
     3661 aaaataacca gtgcctcatc atccggccca agcttttgga aaagctccag aaattcgccc
     3721 gcagaaggct gggaggtcgt ggggaaaacc ggattatttc tcatgtattg atagtattcc
     3781 tggttgctaa atccgtaccc ttccttaaaa tcccgatcgg gcagatgaac gtttagtgcg
     3841 accacattga tatcgtattg gcgaatgatg aaaacaggta aataggcagt gctgtcggtg
     3901 attagaacgg ttgtcataaa taagggctcc ttatcgtcag aatattcaat aaacaacagt
     3961 atcgcacaca gccttgtatt tggcaagcgt cagcaatggg agttacgctt cataaacatt
     4021 tcctgctttt gtttcattga tgatacggta atggtgttat aatgattcaa gaatacggta
     4081 caggaggaac gaggatgagc ggaccgagag tagtcaggat tccgttacgg tttgccaaca
     4141 gttatttact gatcgatcaa aaaaccatta tcgttgatag cggtgatccg ggctttagtt
     4201 ccggtattct gaaagccatg caggagaatg gggtaaaaaa ggccgatgtc agcatgattt
     4261 ttttaaccca tgggcatatc gatcatttcg gcagtaccta tgagttgaaa aaacatttgg
     4321 atgtacccgt cgccatccac acaagcgatt tgttgtattt tttaaacgga acccaggcgc
     4381 cgctacaccc gaccaatgca ttggcagcca tgattaaagt gatcggcaaa aatatgcagg
     4441 tcaaaaagcg gtacggaatt aaagcggaga ttgttttcga tgaggaattg aaattgcatg
     4501 agtatggggt tgacgcattg gcagtcgcta cccccggtca taccttggga tccgcttccg
     4561 tcgtattacc ggatggttcg gccgtggttg gcgatctgtt ggtgcgcagg aattttctgt
     4621 ttggacaggc atgtatcccg ccatttctcc atgatgggca aaagcatgct gagagtatgc
     4681 gcaaattata tgatatggga gtcaagaaat tttatcccgg gcatggcaaa gtccttgatt
     4741 tagaagaact gaactggcga ttgcagccgc agcattatac gcttcaggac taacccacga
     4801 taggggtctt ttgccgaatg atttgctcat aatacttgtc cagctgattg attaacaaag
     4861 ggattgagga gtagactttc cctttgccgg cctgttcgat taacgctgcg ctttcggaaa
     4921 tttgcataaa accgtacatg ccggcggtac ctttgacggc atggcttaat tcctgaatgg
     4981 cagtcatatc atgctggtcg actgccttgt gcagatcggt cagcatctct gccaggattt
     5041 cgatgaattc gggtaacagt tggctgatta aatcgtcagc gggctgggaa ctgctgccag
     5101 gagggtcgat tttcaaatgg gacttaatct cctgggccaa ttcatcagcg gtaaacggtt
     5161 tgggcagata ggagcaacat ccgcagtcca tacatttttc aatatcataa cgaagggtat
     5221 tggcggtaat ggcaaccacc gggatatgtt tgtaaaggct gttggcgcgg attttccggg
     5281 ttgtttcgta gccatccagg accggcattt gcatatccat caagacgagg tgaaattctt
     5341 tttcctgtag aacttgcagg cattccagac cattgcttac agagacaact tcaaaaccat
     5401 aactgaccag catctgggtg atcagttttt gattgagttc gttgtcttcg accagcagaa
     5461 tattgaccgg caggaattga ttaaacagat aagcatggtt ttcctcattg atacagccgg
     5521 taccttgttt ggaatcaaga gattggtctg tgctggtaac catattgtgg ttgtggtgtt
     5581 cattcatttt agctatggaa aggagcacgt caatggaagt cagcagcttt tctccgcaag
     5641 ttttgatggt ttcaagatga gccttttgct caacatcgag cacactttgt gcaagcaggt
     5701 cggaggaccc cataattcca attatcgggg tgcgcaattc atgaccaatg ttgtcaaaca
     5761 aagggctttt ggtaaatcgc caattagcgc ttgcacgctc gttttccagt aatttgttca
     5821 gactttgact aatgttgcgg tgctgaaaat agaggagtga aaccaaaaga agcgcaacga
     5881 gcgatgaaac catcggaaaa atgaccggat gatcaaaaaa acaagcagca tgcaactgac
     5941 cgtaaaaata gttaagccag gtaagtataa ccataatgat gactaatttg caggaccggg
     6001 cggcagtgtt tttcattttt ttctcgatca taaaacatac ctccataatc caagaaatac
     6061 ggacatcagt aaaaggggta taattagatt acaacattta ccataaaaaa aatgtctgct
     6121 tgtgtttttg aaaacacata attattcggt tttgttggcg aatataggca aagaacttcg
     6181 taaacaggcc gatcatttaa aaaatagctt gaaattaatc attattttcg attttaccag
     6241 gatggcaatg caagaaattt ctgcttattg ggtattattg ataactatta cgatcaggca
     6301 acgggtcata gggcaaataa ccagccggga ggggcatata acctgaaccc ttttcgatac
     6361 gcttactatc gttgcttcaa aaaataactg cagtcatacg caaaggagga gtagggtttg
     6421 tataagctga gattggcaaa aagaatgatg atgttgggtt tgtgtttgct gctggtcggg
     6481 gcggcagtca aaccggtcgg cgccgttccg gtgtctggca gcagtgattt cagcatctac
     6541 atgaataatg agcaactggt catcccggcg gatgatcagc aaccagtcat cattgatggt
     6601 cgtacctatg taccgctgag agtaatcagt gaaaacatgg gggccaaagt ggagtggatt
     6661 gaagcggaac ggcaggtaat catcctttgg aaaaaaacaa gcattgatat ttggccagat
     6721 tcccggggag aagatgtgca aatcatcatt gacggaaaga ttcttgagat cccggaggat
     6781 ttaggcaaag catacataag tgaacgggga aggaccatga tccccttgcg ggcagtaggg
     6841 gaagccatgg actgtgcagt ggattggatt gatgcatcga ggaccgtgga gattcattat
     6901 gaacggccgg ttagcgaagc aacagagcca atcaaggaga cgcccccgga accaaccgat
     6961 gcttcagatc aggacaacct ccagatgttt aaagatctgg ccgctttccg gaccaattta
     7021 aaaatgttgg atggcaaggt catgaactca gaagagttgt taacccgtca accgactgat
     7081 ttcagtgccg agcagttggc ggttttgcaa acctatttgt cccagctcag caaataccag
     7141 gcattgatta ccctgccgga cggatctgca gtcaatagtg cagaggttag catattgggc
     7201 gattcttatc taaccgcccg gcaactgact gcctggatcg agcaggagac gccgcgtctg
     7261 atggccaagg cagctgccaa cggggtggag tttaaaccga ttccggatct ggcccggctt
     7321 tatattgata ttggcaagga atatggcatc agaggtgaca tcgctttttg tcaagccgcc
     7381 aaggaaaccg gctactggca gttcaccggt tcggtaacac cggaacagaa taattactgc
     7441 ggtctgtggg ctaccggctc tccgctgacc gggcaggaat cattgaacgg agccgaccct
     7501 gaacaagtga catttgttgc cggtttgcac ggtgccactt ttgcaacccc ggccgtcggt
     7561 gtcgaagccc atatccagca tctttatgcc tatgccacca agaatccttt gccgaaagga
     7621 aaagtgctgg ttgatccccg ttttgccctg gtcagcaaag gagtcgctcc cacctggctg
     7681 caactcaatg cgcggtgggc agtacctggc actacctatg gacaaagcat cctggcagat
     7741 tattggctaa tagcagcgaa gtaatcgctt tttattcgtg ggataaagat ggaaacaaat
     7801 caccccgctt ggtcatagag acaaagcggg gtgatttctt ttacgggaaa atacaccaag
     7861 aattattttt taagggcagc agcttttttt gccagagagc caaagggtgc ccccaccggc
     7921 agaacggtga agccataatg agtattcagc accgtagctg ctgaaccgta gaaagacata
     7981 atggcgatgc ccagttcaga gaaagccgcc agcatatgcc acgcttctcc gccccagccc
     8041 agcgtactca tgaacaggcc caggaagagc aggtcaatca ggaagaagat aataaacaat
     8101 accttgttgg ctcccatagc accgaccgtc atgaacgccg taaagataaa ataccccaga
     8161 aatgcatatc ccaactgatg gggatcaaat ccggcacctt ccccaatcgt tccagttacc
     8221 ttcatatacc aggtcatgcc cactgcgaac cagaaaaatc catatccaca gaaagccgtt
     8281 gcgccgaagg tattgtcgtg cttgtaatcc atcaggccag ccacaaattg cgctgcagca
     8341 ccgaggaaaa tcgcccaggg aataatgtag gctacaccgt ctgtgactcc cagcttttgg
     8401 gttgaagcta ccagggttac cattgccaga cccaataagc ccagcggcga aggatttgca
     8461 accaggtcct gcacatgacc tgaaacgcgt acttcagaaa taggacctgc tttttcattt
     8521 gtgttgctct ttgacataat ttcctccccg tcattattca ttgatcaatc tctcaaccgt
     8581 tgggtcgatg attatgtaac aaacgtcaga aacaattgcc ctcctttcga taaatatcta
     8641 tacaagttca acaaatcttt taaaaatcct gttttttgta taaatgttta tttcgggtgt
     8701 tacatccggt aatcaacatg atttccaagg caatatttta atccaataac atacgcgaaa
     8761 taagcaaaat tatcggaaat taggatgatt taaattctaa taggaaaaga aggaattacc
     8821 ttgtgaacta gcgaaaagac actcagggaa aaatacattt acatttattg gggcagcagg
     8881 aaattgcggt tccggaatcg ggggtggtaa ggagcatatt ttgttcaaac atgatgggaa
     8941 aaaagtaaat ctatttgagg aggtaaaaga gtgaccgcta attttgcgta taagaatacg
     9001 cgcgacctga agtttattct caaagagtgg ctgcctactg aagaagtatt tgcttaccca
     9061 cgttttgccg actattattc caaggaagat gttgactctg ttttggatac ctgtctcaag
     9121 atgtccaaag aattgatcga acccagcaac gaggacggca acgccaatcc ctgccgatat
     9181 gacaacggaa aggttgttac acctgccagc tttggcccgc tgtataagat gctgcagtcg
     9241 gaagggtggg ggaccagcaa cattgatgag gcggaagacg ccatgacatt gcccacaatt
     9301 ttaagatttg cggtttggga actgtttttg ggagccaatc cagctttcct gccctatatt
     9361 atgttaacca ccggagcggc ggaattgatt caaagttttg ctgcgccgga cttgaaggaa
     9421 atatttttgc ccaagatgat ggacggtacc tgggccggaa ccatgtgcct gacggaatcg
     9481 acggccggct ctgacgtggg ggatatcttg tccaaggctt atcctaccga tgatccccgc
     9541 atctttaaaa tcaaaggcag caagattttt atcaccggcg gtgacaacga ttttaccgat
     9601 aacattatcc atctttatct ggccaaggtg gaagggtcgg tcaaaggaac gaaaggcata
     9661 tctttgttta ttgttcccaa gtactgggtc aacgaggatg gcagcctctc agacaacgat
     9721 gttcagacca ccggcattga gcataagctg ggccagtacg ggtccgtgac agcatctctg
     9781 agcatgggcg aaaacggcaa ttgccgcggc tggctgttgg ggaaaaaccc gctggagaat
     9841 gacggagtcg gtgaaggtat ggcgcagatg tttgaaatga tgaacggtgc gcgtatggag
     9901 actggtctgg cagctttggc ctgcactgcc aatgcctttt acaatgcccg tgactactgc
     9961 caggagagaa tccagggacg attaatgagt gatcccaggg gcgatcgggt cagcataatc
    10021 agacacgagg acatcaaaag gacactgctg atgggcaaag ctcatattga agccatgcgg
    10081 gccatgatgt ataaagcttt tctggcgttt gatgtgcgct tttgtgatcc tgatcccgag
    10141 cagcgcaaaa aagccaatgc attggtcgag atctccacgc cattgtgcaa agcgtatccc
    10201 agcgacgaag cctggtggtt gatcggggaa gcgattcaag cttatggggg ttatggttat
    10261 tgtgaagaat acccggtcgc gcaaattgcc agggatgtta aaatctattc catctgggag
    10321 ggaaccaatt ttattcaggc catggatttg gtcggacgca aatggaccat gggcaaaggg
    10381 gcggctttcg ctgcattcat gcaggaaatg cgggattttt gtgcggccag caaacccagt
    10441 gcagaagcag cggcaactgg cgaagccggc gcgatggatc ttagtctgaa caaagagttt
    10501 gctatcctgg aaaaagcact ggacgcctat ggacaaatgc agatggccat cggtggctat
    10561 ttcagcaaga aagaattcag catgatgccg ttgtatgcac gccggatcct gacggccaca
    10621 tcgcagctgt atgcaggcag atgtattctc gatcaggctc tgctggcggc aaagaaagcc
    10681 cagcaagtag gaccggatca ttatgactat cctttttata ttggcaagat cctgtcagcc
    10741 aaatattatg tccgcaatgt tgtacccaat gtttggacag tcgctgatat tgttaaagat
    10801 ggtgataaat cagccctcga tatcccgctg gaatcttttg catactaaac gataaaacaa
    10861 tcacgataag atttaaccag gactgccttc ggcagtcctg gttagtttct ggagagatta
    10921 gcgggataac gccaacagtg catacaattt atatcgtatg gctgggagca gatcctctgt
    10981 tgccccgctg ttgccatcaa cctccgccag gaaggcagtg atgtcctgat gcagttgcgg
    11041 ggatccgtgg tggaccagaa gctcatttat atctctcaat gaatccagca ggctggcatg
    11101 gaactggttt tcgccggcgc tgggctgact ttgccgggga tattgatcgt tcatgcatca
    11161 tccttctctc agtctgatat ggggtaccaa tacatcctgt atgtctccca cccggaaaag
    11221 gaacagatct ccgtcctctt cctcgattat gagttgctgg cctgcctgtc ctggcgtatg
    11281 atccaacagg gccaggtacg cgtcgggaaa tgactcctcc tgggtcaact ggatatgctc
    11341 cacccgcact tccaaatcct taaactgccg actgagatta cggggagcca aagtccgggc
    11401 caactgattt ttcatttccg tcaacatttt ttgataattg tccaaggtgt cttgaatctg
    11461 tgcatattgc tgctgtgttt tctgaatgtg ctctgcctgg ttggccagat gatcgagctg
    11521 ggtttccagc cagccgaact ggttgcgcat ctgtgccaac atgttgttca attggctgta
    11581 atccataggg ttgctcttac taatattttt gaaggcgggc caggtatcgt gcaaaaaatt
    11641 ctcccctgcc tccaggaaaa ttgagaactc gggattggct gacttgccat gttgcttgat
    11701 cagaacttga agattctttt gtgctttgcc caacagatca atcacttctt tgagcgagga
    11761 agcgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagatt cttttgtgct
    11881 ttgcccaaca gatcaatcac ttctttgagc gaggaagcgt ctgcagaatg ggcataattc
    11941 atttccagca atagaggttc cgggctgccg ttgcggcgat tggatctttc cagcaggaaa
    12001 agcagcgctt gcagccagct ttcgaaatga tgcacggctg cctggaggcc ctttcggtct
    12061 ctgagcttga tgttgagcgc caacagttcc gtgtatatca tcaactgggg ccgcaggtcc
    12121 tggcggtacc attcatgcaa tgcgggagaa tattttttat tgtttaattg gtgctcggtt
    12181 ttattaagtt ctgccaacca cattgcccaa tcatcggcgc gcttatcatc attgccggag
    12241 attttaccca ccagcgcggc aaacaattcc aggttgatca gcaatcgttt ataatccctt
    12301 ccccaagtgc tgctgtccgg gtcccaactg ttccaaagat ttttcaaaat cgattgaaac
    12361 ggataaacct tcatgctttt tatgagttct ttttcggtgc ttagggtttg acctgtgtgg
    12421 agcaagtcga ggcacgctgc cagcaccgtt tcggtttctt taagagcata tatcagttcc
    12481 agcaacaact gcgcttgatt gcgttcgagt gcaaaatgga gatggttaat ctgttcctgg
    12541 ttgtctttga gcaagtgcag agcgtttttc acctggttat aaatttgcgc ggaacgtttt
    12601 aggtttttga taatatcttc ccgtaaagct gattcatcac gggcgatccc ataaagcctt
    12661 tttatctccg cttcccattg ggctcggtag agggggatga attgttcatg aaagcgctgg
    12721 taataatagt tgtttaaatc caggataaac tcatccacag tattcacatc aacacccggc
    12781 agacattgac tctggatttg cagttcgtcc ttgatctgac gcaaaaaaga tgagagttca
    12841 agcatattct cgcgtacctc tggttccggc agatgcattt atttcacccc caaaaatgaa
    12901 cccagcaaca attgctggag aataggattg ttttcattct tgaaccgctc agtttgctgt
    12961 tgatatagct cccggatgga gcctcgggtg tattggctgg cgattaacca cagtcggtct
    13021 gcttcctttt gttgttcatc ttcgactgac gcagacaact gttcataaat cgactgattc
    13081 aaaaaggagg cagccgggaa ccattggaaa agcgacaaat ggttctgcag gagttttccc
    13141 gcccaggacc tggtcgggac aattttgcgg tgacgggccc caaaaagcag gagtctcatg
    13201 ttggtcaggg cacgatgggg catggcggtc aagtcccggt caatctccgc caacagactg
    13261 gctacagtgg gttcatccgc aaccgcatgg tctatcaggg agctgaccca ttgaataact
    13321 tcattcaacc catgttgtct gcagatatga gccagctcga tcatggtata ttccggattg
    13381 ctgtcatgac tggtctgcag actctgatac agatgcagat aagcctgggc atttttatca
    13441 gccgggattt ccggataata gcgatgatac aaggatgcac tgtagcgata aggcatagcg
    13501 ttgctgatta aagcatacaa tgacagggat ttggccagat tgcggctgat ggctttaaac
    13561 aagagggcat catcttcaac agacagttca ctgcggccgg ttgtccattg ctgatagaca
    13621 aaatgctgta aagccaaaca ttcctccaac atgcccagca tgaaaagctg ttctgcgctc
    13681 tccagatcgt agcccagtgc caggaaaagg ttttcccggt gatcaatcgg cggtgtcgcc
    13741 aagcgttgca cggtttgttt aagcagcgcc gttttgtctc cgaaaagggt tggacaagac
    13801 ggtgtgggcc ggtataaaag tccgtaatgg ctctgggtgc gttgctgatg atgttgtgcg
    13861 ttcttgtctt tcagcatggt gcgcaagctt tggttggccg tccggtccag taaatattta
    13921 cagtgctcct cctccaggtt ggccaaaatt tcctggtact cctgggcaaa gtcctcgcgg
    13981 taaagactta ttaaaaactg caaacgctgg gaagaaggct tttccgcagt gatgatgcgg
    14041 ggcagaaatc gcaaaaatgc ctggtacaga ggcaacggac cctgcaacag ttgattttgc
    14101 acagtctgac tgtccatatt atagaaaaga atttgcagaa aggcggttcg atgcccacca
    14161 taggccagac ccaggcggag aaaatcgtac tgctctttgc tctcaatggc aagttcgcga
    14221 tattcgcagc gggcttgccg caattctttt cgcaattgga cttcttcctg ccagaaggtg
    14281 aaaagatcat cccgtggcaa ttccttccaa cgttgcaaga tgtttcgcat cattcatctc
    14341 cccggtctat ttgccgtaaa ggtcattatt taataattga caggcattga ccgctttttg
    14401 atagtggttt tccgttaaaa agatatagta ataaatggat ttctccttat tggtgaaaat
    14461 acctcccaga aaatccggac ccagtacttc aattttgtca gtactggatt tgttgccgcg
    14521 cagataacgg taactttgaa cccggctgac aaaatcataa cggccgattc tttcaaaatg
    14581 actctttagt cccagttctt tataagaatt acctttttca tatttgtaaa ccacttccct
    14641 ttgtcggggc gcgggcagac cgtaatcggg ctgaaccctg cccaccagca attccagcag
    14701 ggaggtgatt gccaaagggt cgatggttga accatagctg tagtgcaaat agtcatgcaa
    14761 aaaaggagga aattcctcca accccagcaa ggcttgggga tgtttggcaa ggaaatccgg
    14821 attttgtacc agataatcat aaaaatcatc ccgcccataa cgaaaaagag gcagacaatg
    14881 accgtattcc cgatacaaaa cctgttcata ggaatcgtaa aaagaacggt agttttcaaa
    14941 tgatttacgg cgcaatctcc gggcataagc caaaagctgg cgcaactcag cctcatccaa
    15001 tcgaatgtgc cgggccaatt tgtcagttat gatgttgatt gtttccggag cgttagccgc
    15061 gatgcccatt ggtcttgccc ttgttcctct ctgctgaaaa ttggagattt agctgccgaa
    15121 ggtgctgaaa acataaccct gggacagcaa ggtagtgatc gttttttcca gggcttgcgt
    15181 gtcactttcg gatacagcat gcatcaggat tacggcgccg ggatgaatgt tatcgatgac
    15241 atgctgataa acataatctg cgccgggttg ttgctgggga tcccagtctt tccaggccat
    15301 gctccagaat accgatgaat accccagcgc tttggtccat tgcagactgt tggcattaaa
    15361 gttgcccatc ggcggacgca ggtacttgtc catgtcctga ccggtcaagt cacgataaag
    15421 gctttccaga ctggtgattt cctgctccag cttactctgg ctgatagtgg agaaatccgg
    15481 atgattaaag ctgtggttac agaccagatg gccttcttca tgcattcgtc tgaccaaatc
    15541 gggctgggtt ttaatatact gtccggtaat aaaaaaagca gctttaacat tctgtgcggc
    15601 cagggtatca agaatctgag atgtataccc caattcatag ccgcagtcaa aggtcagata
    15661 aatattttgg cggtcattgg ggaggatata aaacgcctga tgctgtgcca gcaattcttt
    15721 gtattccgca ttcgtcccgg gcatttgatg ctgatcatta cggataaaat accaggacaa
    15781 ggttggcgat gttgtggagt cagcttgctc atgcgctgtt ccttctgccg gggcagttgg
    15841 ggccttatcc ggaacagttg tggatgcttc cgggacaacc ggatttgccg ggtttgcttc
    15901 cggtgcaggt acatcgttcg tgattggcac tttgactgcc tgttcatcgc tgtcaggggg
    15961 aacacatccg ctcagccata ccaacatcac tgccatcaac aggatcaaac cagttttttt
    16021 catatacatg ctcctttttt aaataaaccg aaataatgca ctgactccat gtatattatt
    16081 ctataataat tatagggaca aaatgatgta atttcattat taatacactt tatttaggga
    16141 taaactgaca tgaaaactat gtttgtacag gataaaaaag caaaatgcaa tcgcaaggaa
    16201 agcaggacga atgatgaatg agcgatttcg aatcaccatt ttcccggtta tgggtttgtt
    16261 gcttttgctg ctggtcatag gacagaactg gccgttgctt acagaaaacc cgaattccgt
    16321 ggtgtatact tatttccaca gctttgtgtc gggagaaatc ggttccggtt atgggcaggc
    16381 tgctgcgggc gaaattccct ttcatctcct cgaaccgggc gatattttgc tgggaggatg
    16441 gcccaactgt gcttacggga aatattccca tgccggattg tatcttggca acggggaagt
    16501 attggaagcg tatgttgatt acggagtttg cgttcagccc ctggaccatt atattcagta
    16561 ttcctatctc agtctggtca aggtgaacgt cagtgaagaa atcaaagctg cggcaatcaa
    16621 agcaggtcgc agctatatcg ggcagacatt ttatccggtt gcttttaagc acaacgatcg
    16681 ctattacaat tgcagcagca tcatctggaa agcataccag gaacaaggca tcaatttgga
    16741 catcaataac gatatctggg tggcaccgga tcgtttcggt gacaatccct atgtaaaagt
    16801 tcttttcaca aaggagatgt aaaccattag caaagcaatt gtgaggcttt atacgattca
    16861 attggcagcg attctggcgg tctggctggg gatgtattat ccgggtctgg atatcatact
    16921 ggcaggactg tttttgctgg ttgtgtgggg ggaggccgat tatcagcggt tttcatggtt
    16981 gcagcaaagc ttgattgcca tcatctggca actgccggcg ttgtttttat ccttgtcggt
    17041 tctgctgggg cttgataaag ccactgattt ttcctattat tttattttta tgctgcagct
    17101 gtggcataca cctttgttgc cgcttacaac agtactgcca gatctgttgc agctggataa
    17161 acctttttat tattatgagc tgtttgtcat gaccccgctg ctgtggatgg tctatttcct
    17221 gccccggctt aaaagattgc tgccaggcag acccataaat taatccgttg ggttgatcgt
    17281 tcatatcctt tgccgacgca tcggcaagga tggcgaaccg aagtccggca acgatcgccc
    17341 caacggattt ttcagaaaca ggaacggtgt tttcttttaa gggctaagag ttgtattcgc
    17401 ttaagattta tctccgcaga ggacttaagt gataacactg ctgcatacct tacaagatcc
    17461 gttgctgctt ctatggttag tatgttcccg aaaaaagctg ctatgcagga aaataatggt
    17521 ataaaaacgg actggctgct tgtgcaaatt ttcttccaaa ccaatccggt tgccatgtta
    17581 gaatgaagaa tatgtttatc ttttaaagga cgaggagttg tgaggatgat atttcagaca
    17641 aaactggacc aggaaggcca aatcggaccc ataaaagtaa aaaaccggat cgtaatgccg
    17701 gccatggcta ccggattggg atcaatcaac ggcgaagtga ccccggccat gatacagtat
    17761 tatcaggaac gggcggccgg cggtgcggga ctcatcattg tagaaattgc ctgtgttgac
    17821 agtccgcaag gacgaggcag tcttacccag atctgtgccg accatccccg ctatgtgttt
    17881 gggcttaatc aattatgcga agcgattcaa tcctatgggt gcaaagccat gttgcagctt
    17941 catcatgccg gacggcaaac cagtccgttc gccacggacg gcatgcagcc ggtagcgccc
    18001 tccgccatag cctgcaagct gatgcgggcc atgcccaggg aattgaccct ggatgaaatc
    18061 gctgccctga agaataaatt tgtcagcgcg gcattttttg ccagtcaggc tgggtttg
//
LOCUS       DAOTHZ010000043        17763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3826_length_17763_cov_6.553479, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000043 DAOTHZ010000000
VERSION     DAOTHZ010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 17763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17763
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_17763_cov_6.553479"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(432..1259)
                     /locus_tag="P5549_06455"
     CDS             complement(432..1259)
                     /locus_tag="P5549_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRW12750.1"
                     /translation="MADIREVVKQKYAEAISGKTGGCCGGGKIDLGGCCCGSASDAIN
                     PITGNLYQLKDLQGMNPDLIATSFGCGNPTALAELNAGEVVLDLGSGAGLDVLLSAKR
                     VGPGGKAYGLDMTDEMLAVARANQVEAGIANAEFLQGHIEEIPLSDNTVDVIISNCVI
                     NLSGDKDQVLHEAFRVLKPGGRFAVSDIVLRKPLPANVQQDMLAWAGCIAGALLDQEY
                     QAKLAAAGFADIEIEVTREYDLGDDMMASLLESLSPQEKEQFNGAIVSAFVRAKKPA"
     gene            complement(1312..1617)
                     /locus_tag="P5549_06460"
     CDS             complement(1312..1617)
                     /locus_tag="P5549_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRW12751.1"
                     /translation="MIDMNRISKVLSDPIRYKILLMLVNQAGGTRACCAGPAEGICNC
                     EIMAVFGMIQSRVSYHMKELVDTGLVREVPEGKWKYYFINEEVLREYVRKLEADFKL"
     gene            1990..3162
                     /locus_tag="P5549_06465"
     CDS             1990..3162
                     /locus_tag="P5549_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRW12752.1"
                     /translation="MVEKVDDNNVTRLQYQDKEILLIATAHVSKESAALVKKVIEAER
                     PDSVCIELDEQRFQNMQNPQAWENTDLIKIIKTKKVGFLAANLLLASYQKQIAKKLDV
                     PVGGEMKQGIESAREVGATLVLADRSIQTTFLRIWRKLSLWEKNKMLVSLLFSFDDEM
                     DDISDQDLQEMLKEDMLNTVLAELREEFPPVGEILVSERDQYLAAKIKEAPGPKVVAV
                     LGGAHVPGVKEEIYKTQDIERISVVPPKSRLSKLTGWIIPALFVALFAYAFAGGVQTG
                     LQQLTVWVLWTSFLAAGFTALSLAHPLSILTSFIAAPFTTLNPLLACGWFTGLTEATV
                     RKPTVADVQNVSQDIFSLKGFYKNRLLRTLLVIFMANLGASLGTFIAGADIIRQLF"
     gene            3422..4027
                     /locus_tag="P5549_06470"
     CDS             3422..4027
                     /locus_tag="P5549_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRW12753.1"
                     /translation="MPKIVDHDAYRDKLIERYFDDFAQRGYSDVTMREIASKLGISTG
                     TLYHYFPTKKNILEHMLQLASRRDISGAMAPINEDTPLEERIRIFSSYIMEKEQWFAD
                     IVLLTIDYYRQQNKEGGFEIVNEADSHYGNGVAEAFGLSTELGFFFTIFFNGLVFHRL
                     AFPDSISFEEQAELFIKVMMAYARAEGIEKNTHPEEADKGN"
     gene            4080..5921
                     /locus_tag="P5549_06475"
     CDS             4080..5921
                     /locus_tag="P5549_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW12754.1"
                     /translation="MMQQKPDYEKEFEQYLAREKHLALMVRSRAAKYGDSKIAVRHKP
                     YGEWIAYTWKEFGDMIDTTAKGLLEFGVKEGEFVGIFANNRVEWAVADYASFTVRAAS
                     VPVYATNSAAELKYIINHAEIRILFAGNQEQYEKALSLLGADCPTLEKIIVFDKIAVI
                     EKRPEVMSFEDYLEMGRKAGREAELEERIARLDSEDLSTLIYTSGTTGTPKAVMLTHK
                     AWFAMLFATGYHIPIIETDVNLAFLPLSHVFERAWSYFILCGNGQVDYCHDTKAINQF
                     LQESRPHYMCSVPRMWEKIYATVQHGISMAPPEQQQGFKWALAIGGQYETLKKEGKPI
                     PAELQQQYAMADEKVLKNIRNIFGGRNKVFNCGGGAFSGEIAEFFFNAGVLLLQGYGM
                     TECFVITVANPEHNKFGTCGPVVPLMDVKFSAEGEILAKSPSMMKGYYKDPELTRESL
                     TEDGYMKTGDVGFIDEEGYLSITDRIKDMMKTSGGKYIAPQHIETLLKEDFYIDNAAT
                     IGDQRQYVSALICPSFEALEGWAKAKGISYASREELVQNPEVIAFYRELVDNRTKDLG
                     QVEKIKKFTLLPNEFAQETGELTATMKIKRKAINEIYADAIEAMYVE"
     gene            6343..7596
                     /locus_tag="P5549_06480"
     CDS             6343..7596
                     /locus_tag="P5549_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRW12755.1"
                     /translation="MLSLRLRQWFFLYMLTLLFIVSAPTLTQASDYGTCDLTLDSRGS
                     AVSQLQQDLASLGYYTYKVDGWFGAKTHDAVVRFQKSQGLKTDGLVGPITKAALNRVI
                     AAAPSPTPASDDGTDDLALDSRGSAVSQLQQDLASLGYYTYKVDGWFGAKTHDAVVRF
                     QKSQGLKTDGVVGPITKSALNQVLTKSVVSPKVSASAKSPTAASISQYNSQEKPIVDA
                     FRANFDGSLAQTLVGRAIWYMEHGYMKYGHSKYPDTGYIDCSNFVSLVYKDFDYSITS
                     AAKNYGSVGEKVAGVYSKKITGTSKYTLVGTEKLKPGDIFTFWNSDDPARTHIGHVAI
                     YMGVINGKPCIINTCSGRPTAIGIINSFAYWYGSSLIEVRRVLPDSAYKAGNKINDQG
                     PVLPKIYQIKPNKPIILPVNLPAGF"
     gene            7832..8710
                     /locus_tag="P5549_06485"
     CDS             7832..8710
                     /locus_tag="P5549_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRW12756.1"
                     /translation="MTRANRFTEKPDRIPSLLLIIFTLALLCMSGCGSQTTQDQAKSI
                     ENYESRTDSAAPLTKNQDETVTGIINAADRKVVQNAEISLKVQEVAAAADKITDLNQT
                     NGGYTVNSHIYRNGEHISADLLLKTPQQKLTSFIASIAALGEVTDKRVSAEDVTEQYY
                     DAEARLKVMRAKEERLLSLLDKANSITDIISIEKELGQTRSEIEVLTGRLQYLSNAVD
                     YSAVHINLIQAVPGAIKPPQGTIGKSVQGLITSLNNMIHFGSNLIVGLFVLLPWLLLI
                     LILVLVFRYFYRRKKK"
     gene            8758..9123
                     /locus_tag="P5549_06490"
     CDS             8758..9123
                     /locus_tag="P5549_06490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRW12757.1"
                     /translation="MDKCVFCDRAEMVLENRLAWAKYDQFPVSPGHLLIISKRHVPDF
                     FAATTEERQAFNELLNEAKNMLEEKYHPDGYNIGVNCGESAGQTIMHLHIHLIPRYHG
                     DMDNPRGGVRGVIPEKRIY"
     gene            9362..11647
                     /locus_tag="P5549_06495"
     CDS             9362..11647
                     /locus_tag="P5549_06495"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRW12758.1"
                     /translation="MIRVEGQTPYQAVDTASFAEDLFVELFQETFGLDKTQYLVNEYP
                     CENIYGDNCYIDYALKTDLEAYAIEIDGEQWHNPALVSVDKYLDDLLKRNSLTWLGWR
                     VYIWTYRQLVNEREKVKSELVQFLGQSPHFKIYDDYLPRQRGTVLELRPHQEETLQRL
                     QELRGEYNSMALIADAQGTGKTTTAVLDACRLGLRTLFVAHTLELLQQAARRFRELWP
                     QARVTIIDDYSGPLDGDVMIASIQGLHNRLSQFAPDHFGYIIIDEAHHAASPSYRQVI
                     SYFQPMFLLGLTATPERHDQESIMELFKNEAHRLDLKTAVEIGELVPIRCVRVKTNID
                     FSSVRINGIRYNYRDLDTCVHVPERNRLIVDTYLSHVPGQKAVVFCASVQHAAEVAQL
                     FQESGINAQLVEGRMKKPLREEILDQYRQGIVKVLCACDILNEGWDSPETAVLFMARP
                     TLSKVIYLQQLGRGTRRAQGKNALLVFDFIDNTVRHNHSVNLHRLFRSREYRPGALVI
                     APQEQMEEEKRRLLSGEKMDTILPHNIFVRDYELVDLFDWQEEIKHMMSLHQLAMELY
                     VDDATARRWVDECKITPGPDLELPMGKVTHRYFSQERLEDIREQLNIPARVPEAIRED
                     FFRYVKAGQMSFSYKPVMLKGMLRLVNDKGQAELGALVSYFRNFYEQRADADLQIEVS
                     GAVINRVKEMNDFDVARLMLTMPFEKFERKFFLEHRKDLNLVGFVPALWKRLTVEDKA
                     ELIEICDRQIERYYARRVGKE"
     gene            11814..12938
                     /locus_tag="P5549_06500"
     CDS             11814..12938
                     /locus_tag="P5549_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HRW12759.1"
                     /translation="MAFFDSVKEAITQKPANLKKPFFYKKDSDAQRQLEKLKEIYKTA
                     PNSIKLKIDQDIKMLSYGIAGEENIAFELSNSLLPIIVLHDLHIEFEDLSTQIDYLII
                     TNKFSLIIECKNLIGNIEVNSNGDFIRTWQYNGKYNKEGIYSPITQNARHLDMIKRIK
                     LTHKNNFVTKALFEKYFDEFHKSVVVLANPKTVINMKYAKKDVKDKIIRCDQLTECIK
                     KMISDSKNALFSEKEMYEIADFYLSLHTPNTVDYTKKYQIDEPAQEESIPTVAPTLAP
                     TTATTTAPKSVPVANPVATPTKPNIDETPLYKALKQYRYDTSKAEGIKPYFIYNNAQM
                     EELISIMPKTFEEIKKVSGFGDVKCQKYGSAILEIINQYK"
     gene            12964..13821
                     /locus_tag="P5549_06505"
     CDS             12964..13821
                     /locus_tag="P5549_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRW12760.1"
                     /translation="MIGIMDSEQYIYKKEVDWSLLHEGFTIPVSFQVRFQEIIGHGLD
                     KGQTKNITLLVENKPFTAQLKNQDFDQSRYPTHKDLLQIRYTRNSLLADILRTIFNSS
                     YEYLKIKRSELGQNNRLIKLPENIREYLVLYTSDSADLFIADCLTIWDLQKEIQVISS
                     YPEEVLEAEINYKNVDPSAGLDLRERIVKVRRFDKAIADNLKLLYDYRCQICGNNFSA
                     KYDTSIAEAHHIDSFIKSLNNDSDNIMIVCPNHHRIIHKTMPTFERSNRSLIFQNGYQ
                     ERLRLNYHL"
     gene            complement(13829..17026)
                     /locus_tag="P5549_06510"
     CDS             complement(13829..17026)
                     /locus_tag="P5549_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HRW12761.1"
                     /translation="MGAGGLSDCGEADGGDDELKKGLYEQVISKQLGQELDQADELLK
                     SVAPIDSAEASRILAKYMVEIIEKGMDNVKDKGGDIQRQIDLANKIVQVIISETQEDD
                     FDMLSVDERGEQLLALLNKQNTVYAVNEKVEIIRPQTSLAQSSLFTGAIHEPSMYTEL
                     KKEIASADRIDILVSFIKWSGLRLIIDELRNFTQRGGRLRIITTSYMGATDVKAIEEL
                     RRLPNTEIKVSYDTKRTRLHAKTYVFYRDTGFTTAYVGSSNLSNAAISSGLEWNVKVT
                     AKDLPETIRKIDATFESYWNSTDFEDYNETQKTRLKQALTAEKLTGGDSVTLFTFDIR
                     PYPYQQEILDKLEAERKVRGYYKNLIIAATGTGKTVISAFDYKRFRQANSGQPCRLLF
                     VAHREEILKQSWLCFQGVLKDANFGDLFVGNYKPDSLDHLFISVQTFNSQELQTKTSP
                     DFYDYIIVDEFHHAAAPTYQKLLAYYQPKILLGLTATPERMDGKSILEYFDNRIAAEI
                     RLPEAIDRKLLCPFQYFGVTDTVDLNELKWVRGGYDKSELSNLYTLNRAAADRRANMI
                     IASLVKFVTDINDVKGLGFCVSVEHARYMAEYFNGLGISSMYLVGTSQDEERHTAKQK
                     LVSGQVRFIFVVDIYNEGVDIPEVNTVLFLRPTESLTVFLQQLGRGLRLADNKECLTV
                     LDFIGQANRKYNFEDKFAALLANTNKSVQREIKDGFASLPKGCYIQLERKAKEYILNN
                     IRSSFGVKAGLVSRLATFTEDSGLPLTLANFTSYYHLDVRSIYKCGSFSRLCVMAAVR
                     DDFVEPQEEVLTKAFSRICAIDSRRWINFLFKVFSDLDGLDFEVLCESEQRMLQMFQF
                     TVWQKPVEECGFVSLKEGLQQIRNNPVLCAELIELLKYNLNHIDFIDEPVELGFDCPL
                     DLHCTYTRDQILVAMDFMKPATVREGVKYLPEKSADIFFVTLNKSDKDYSPTTMYNDY
                     SINEWLFHWQSQSTTSETSPTGQRYVHHRQRGSKVLLFVREFKTDIAGAAPYTFLGLA
                     DYVKHEGSRPMNVIWKLHRPIPAKYLKKTNKLVVG"
     gene            complement(16969..17376)
                     /locus_tag="P5549_06515"
     CDS             complement(16969..17376)
                     /locus_tag="P5549_06515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HRW12762.1"
                     /translation="MKHLDVVAAIIIHEGQILCMQRGQAKQEYISYKYEFPGGKIEAG
                     ETRTEALMRELREEMNIEITIEEKDYFMTINHVYPDFELTMHSFICHVDTKKFVRREH
                     VNHQWVKPQELKTLEWAPADYPIVERLMEEMMS"
BASE COUNT         4887 a         4415 c         4010 g         4451 t
ORIGIN      
        1 gcccttatgg ataaattcaa cctgactgtc ccggctcttc atttccaatg aatctttgtt
       61 ttaacttatt gatagaggtt aattataatt aagtaacctg acttttaatc agggcgtccg
      121 gcgttcgagt cgccgatgac ccaccatttg aaacgcagaa ccctccgatg ttcggagggt
      181 tttttgttgg ctgttgattt tattatttgg cgtaaaaaag tcacacgtgt tacaccatga
      241 cgtttttaat gtaacaaaat aaaactgaag atcttgaact gtagcctact ggaaaaagcc
      301 ggtggacttt attggttaac cattcgtcaa atatccagcg ttacaggagt gaatccaatg
      361 aatgtacaaa gagcttgcgt cttgttacgg aaagaatgtt cccgtgtaac acgtgcaaca
      421 gtgcaacaat attacgccgg ttttttcgcc ctgacaaaag cgctgacaat cgccccgttg
      481 aactgttcct tttcttgcgg agaaaggctt tccagcagac tggccatcat gtcatcgccg
      541 agatcgtatt cgcgggttac ttcgatctca atgtcagcaa atccggccgc tgccagcttg
      601 gcctgatatt cctgatccag cagggcaccg gcaatgcagc cggcccaggc cagcatatcc
      661 tgctgcacgt ttgccggcag gggttttctc aacacaatat ccgatacggc aaatcttccg
      721 cccggtttca acacacggaa tgcctcatgc aaaacctggt ctttgtcccc ggacagatta
      781 atgacgcagt ttgagataat aacatcaacg gtgttatcgg aaagagggat ttcttcgata
      841 tgcccctgca ggaattcagc attggcaatc ccggcttcga cctggttggc cctggcaacg
      901 gccagcattt cgtcggtcat atccagtcca taggcctttc ctccagggcc gacccgtttg
      961 gccgacagca ggacatccag tcccgcaccg ctgcccagat ccagtaccac ctcaccggca
     1021 tttaattccg ccagcgcagt gggattgccg cagccaaagg aggtggcaat taaatcgggg
     1081 ttcattcctt gcagatcctt gagctgatac agattgccag tgatcggatt gatggcatca
     1141 cttgcactgc cgcagcagca tccgcccaga tcaatctttc cgccgccaca gcatccgccg
     1201 gttttcccgc tgatggcttc ggcgtatttt tgtttgacga cctcacgaat atccgccaaa
     1261 acatttactc ccttcatatt aatttatgat ggttgataag ataaaaaaaa tttatagttt
     1321 aaaatcagct tccaacttcc tgacgtactc ccggagcacc tcttcattga taaaataata
     1381 cttccatttg ccttccggga cttcgcgcac cagtccggta tcaaccagtt ccttcatatg
     1441 ataagaaacc cgcgactgga tcatgccaaa cactgccata atctcacagt tgcagatccc
     1501 ctccgccgga ccggcacaac atgcccgagt ccctcccgcc tggttgacca gcatcagcaa
     1561 tatcttatag cgaatcgggt cacttaacac tttggaaatt ctattcatat caatcatggt
     1621 ttgatcatat caaaattatt tgatgtaatc aagtgggcgc gttatcatta aaataaattt
     1681 tgattttaaa cagtgcgcaa ggcaatatct tatcagaggt aaattaccgg caaaccactg
     1741 ccttgtcctt tacttccgcc agcaataact ttgagctggc gaaggaagta tttcaatcaa
     1801 gagaaacagt atctccaagg aggttataac cccatactac attaacaatg cctgcactcg
     1861 gaatggcaaa gcctgcgcag gaaaactcaa tatcatgctt taaaaccaaa cctacaaatg
     1921 ggccagttgt tatcttgcct gaaatatagt atctttatga agtcgcgaag ataatcttga
     1981 atcagttgga tggtggaaaa agtggacgat aacaacgtaa cccggctgca ataccaggac
     2041 aaggaaatcc ttctcatcgc tacggcgcat gtttccaaag aaagtgccgc gctggtcaaa
     2101 aaggtcatcg aggctgaacg ccccgacagc gtctgcatcg agctggacga acagcgcttt
     2161 cagaatatgc aaaacccgca agcctgggag aacaccgatc tgatcaagat catcaaaacc
     2221 aaaaaagtcg gcttcctggc cgccaacctg ctgctggcct cataccaaaa acaaattgcc
     2281 aaaaagctgg acgtgccggt aggcggagaa atgaagcaag ggatcgaaag cgctcgggag
     2341 gttggggcca ctctggtgct ggccgaccgc agcatccaaa ccacctttct acgtatctgg
     2401 cgcaagctca gcctctggga aaagaataaa atgctggtca gcttgctgtt cagcttcgac
     2461 gatgaaatgg acgatatttc cgatcaggat ctgcaggaaa tgttaaaaga agatatgctc
     2521 aacacggtgc tggcggaatt gcgggaagag ttcccgccgg tgggcgagat tctggtcagc
     2581 gagcgcgacc aatatctggc ggccaaaatc aaggaagccc ccggccccaa ggtggtagcc
     2641 gtgctgggcg gcgcccatgt tcccggcgtc aaagaagaga tttataaaac ccaggacatc
     2701 gagcgcattt ctgtcgttcc gcccaaaagc cgtctgtcca agctcaccgg ctggatcatc
     2761 ccggccttgt ttgtggcgct gtttgcctat gcctttgccg gcggcgtgca gaccggtctg
     2821 cagcagctga ccgtctgggt cctgtggacc agttttctgg cggccggatt cacggcgctg
     2881 tcgctggccc atccactcag catcctgacc tctttcatcg ccgccccctt caccacgctc
     2941 aatcccctgc tggcctgcgg ctggtttacc gggctgacgg aagcgacggt aagaaaaccc
     3001 accgtggcag atgtgcaaaa cgtctcccag gacatcttca gcctcaaagg tttttataaa
     3061 aaccgcctgc tgcggacgct gttggtcatc ttcatggcca atctcggtgc ctccctgggc
     3121 acctttatcg ccggagcaga catcattcgc cagctgtttt agcgcacagc gggggtacgc
     3181 ttgttacgcc ggaacgttct ttaatgtaac aagatttcca ttgacaaata ttcattatcg
     3241 tgataaatat taattacatt atttttttat aacgaccgtt cgatataaat tatactttcg
     3301 atagacaatc ttttgttggt catccacccc tcagaataaa tttcagtgaa tacaacgccc
     3361 ggacatgaca gaaaatacgt tagcagtttc tttaaatgcc cccgcgcaca ggaggttcat
     3421 tatgcccaaa atcgttgacc atgacgctta ccgggacaaa ctgattgaac gctactttga
     3481 cgactttgcc cagcgcggat acagcgatgt caccatgcgg gaaatcgctt ccaaattagg
     3541 gatttccacc ggaaccctgt accactactt tcccaccaag aaaaatattc tcgaacacat
     3601 gctgcagctg gccagccgcc gggatatttc tggcgcgatg gcgcccatca acgaagatac
     3661 gccgctggaa gagcgcatcc gcatcttcag cagctatatc atggaaaaag agcaatggtt
     3721 tgccgacatc gtgctgctga ccattgatta ctatcgtcag caaaacaaag aaggcggctt
     3781 tgaaatcgtg aacgaagccg actcccacta tggcaacggg gtggccgagg cgtttggcct
     3841 gtccaccgaa ctgggttttt tctttaccat ctttttcaat ggcctggtct ttcaccgcct
     3901 ggccttcccc gactcgattt cctttgaaga acaagccgaa ttgtttatta aagtcatgat
     3961 ggcctatgcc cgcgccgaag gaattgagaa aaacacccac cccgaggagg ctgacaaggg
     4021 gaattaatga tctgcttaca ggatgcagcg aatgtttaaa aagaaaggat tgatcgtaaa
     4081 tgatgcagca aaaacccgac tacgaaaaag aatttgaaca gtatctggcc cgcgaaaaac
     4141 atctggcgct gatggtacgc agccgcgctg ccaaatacgg cgacagcaaa atcgccgtac
     4201 gccacaaacc ctacggcgaa tggatcgctt acacctggaa agaattcggt gatatgattg
     4261 ataccaccgc caaaggcctg ctggaattcg gcgtcaagga aggcgaattc gtcggcatct
     4321 ttgccaacaa ccgggtcgaa tgggcggttg ctgactatgc ttccttcacc gtccgcgccg
     4381 cttcggtgcc cgtgtatgct accaactcgg cggccgaact gaagtatatt atcaaccacg
     4441 ccgaaattcg catcctcttt gccggcaatc aggaacagta tgaaaaagcc ctgtctttac
     4501 tgggtgcaga ttgccccacc ctggaaaaaa tcattgtctt tgacaaaatc gctgtcattg
     4561 agaaacgccc cgaagtcatg tctttcgaag actatctgga aatgggccgc aaagccggcc
     4621 gggaagccga gttggaagaa cgtatcgccc gactggactc ggaagatttg tccaccctga
     4681 tttacacctc cggcaccacc ggcaccccca aggccgtcat gctgactcac aaggcctggt
     4741 ttgccatgct gttcgccacc gggtaccata ttcccatcat cgaaaccgat gtcaatctgg
     4801 ctttcctgcc cctgtcccac gtctttgagc gggcctggag ctactttatc ctctgcggca
     4861 acggccaggt ggattactgc cacgatacca aggccatcaa ccagttcctg caggaatctc
     4921 gtccgcacta tatgtgcagc gtaccccgca tgtgggagaa aatctatgcc accgttcagc
     4981 acggcatcag catggcgcct cccgaacagc agcagggatt caaatgggcg ctggccatcg
     5041 gcggtcagta tgaaaccttg aaaaaagaag gcaagcccat ccccgcagaa ttacaacaac
     5101 agtatgccat ggccgatgaa aaggtcctga aaaatatccg caacattttc ggcggacgca
     5161 acaaagtctt taactgcggc ggcggcgcct tctccggtga gatcgccgag ttcttcttca
     5221 atgccggcgt gctgttgctg cagggctacg gcatgaccga gtgcttcgtc atcaccgtgg
     5281 ccaaccccga gcacaataaa ttcggcacct gcggaccggt tgtaccgctg atggacgtga
     5341 aattctcagc agaaggcgaa atcctggcca agagtccctc tatgatgaag ggctactaca
     5401 aggatcccga actgaccaga gagtccctga ccgaagacgg ctatatgaaa accggtgatg
     5461 tcggctttat cgacgaagaa ggctatctga gcattaccga ccgcatcaag gacatgatga
     5521 agacctcggg cggcaaatat atcgcgcccc agcatattga gaccctgctc aaggaagact
     5581 tttatatcga caacgctgcc accatcggcg accaacgtca atatgtctcc gccttgatct
     5641 gtcccagctt cgaagcgctg gaaggctggg ccaaagccaa aggcatcagc tatgccagcc
     5701 gggaggaact ggtgcagaat ccggaagtca tcgcgttcta ccgggaattg gtcgacaatc
     5761 gcaccaagga tctgggccag gtggaaaaga tcaagaaatt caccctgctg cccaacgaat
     5821 ttgcccaaga aaccggcgag ctgaccgcca ccatgaagat caaacgcaaa gccatcaatg
     5881 aaatctacgc agatgccatt gaagccatgt acgttgagta gagcctccgg ctcatcaaaa
     5941 taaatctccc caatgctgaa tcccggaacc gctccgcctg gttccgggat tgttatatcc
     6001 atcgcgcaag gcaagaaccg tccccaactt gcgcaagttg gggacggttc ttgccttgct
     6061 ttctgttgac ggtggggatg ctgccacatg acagctctac tcccacctgt ccattaccat
     6121 gaattaccat caaatgtctt tctgacgcgc aaatgcagga ttttccgccc attaagccct
     6181 ttttatgcct gatggtgatc gaattgtcca tatttaccaa aaggtagtaa cccatttggg
     6241 ccgaattgtt gctagaagga ggtgagaatg gaattattta aaaactgcat atgaaaaatg
     6301 gatattgtac tatttattgc tgactttcga aaggagtaat ttatgctgtc actacgttta
     6361 cgacaatggt tttttttata catgttaacc ctcttgttca ttgtttccgc acccacactg
     6421 acccaggcca gtgattacgg cacctgcgat ttgaccttgg acagccgggg ttcagccgtc
     6481 agccagttac agcaagatct ggcttcgctg ggctattata catataaggt ggatggatgg
     6541 ttcggcgcca aaacccatga tgcggtcgtg agatttcaaa aatctcaggg attaaaaacc
     6601 gacggcctgg tcggccccat caccaaggcc gcactgaacc gggtaattgc tgctgcacct
     6661 tcgccgacgc cagccagtga tgatggcacc gatgacctgg ccttggacag ccggggttca
     6721 gccgtcagcc agttgcagca ggatctggct tcgctgggct attatacata taaggtggat
     6781 ggatggttcg gtgccaaaac ccatgatgcg gttgtgcgtt tccaaaaatc ccaggggctg
     6841 aaaaccgatg gcgtggtcgg ccccatcacc aagtcagcgc tgaaccaagt attaaccaaa
     6901 agcgtcgtct cccccaaggt ctcagcctcg gccaagtccc ccacggcagc cagtatcagc
     6961 caatacaaca gccaggaaaa acccatcgtc gacgcattcc gggccaactt tgacggcagc
     7021 ctggcacaga cgctggtcgg acgggccatc tggtatatgg agcacggcta tatgaagtat
     7081 ggtcacagca agtatcctga taccgggtat attgactgct ccaactttgt ttccctggtt
     7141 tacaaagatt tcgattattc cattacgtcc gccgccaaaa actatggctc cgtcggcgaa
     7201 aaagtagcgg gcgtctacag caaaaaaata accggaacat ccaaatacac cctggtcggc
     7261 acagaaaaat taaaacccgg cgatatcttc accttttgga attccgatga cccggcccgg
     7321 acacatattg gtcatgtagc catctatatg ggcgtgatta acggcaaacc ctgcatcatc
     7381 aacacctgct ccggacgccc gactgcgatc ggcatcatca acagttttgc ctactggtac
     7441 ggttccagtc tcatcgaagt ccgccgcgtc ttgccggaca gtgcgtataa ggcaggcaac
     7501 aaaatcaacg atcaaggccc ggttcttccc aaaatatatc aaatcaaacc caacaaaccc
     7561 atcatactgc cggtgaatct tcctgccggc ttctaaaaaa cagttgaaaa actcagaaac
     7621 accaaaaacc tcgccacact gcgaggtttt tggtatgcct aaacctcccg atcttatgct
     7681 tgcccatgga ctttgtccgc cagcatgtat tatcgtggga tatatctttg ccttatccgg
     7741 aaaattccac atcataaatt gaattacctg cttgatgtct tacgctataa tggaagcaga
     7801 tgtaaaacct caggaaggag atgtttgagg aatgacccgt gccaatcgtt ttaccgaaaa
     7861 acctgacaga atcccgtcgt tgctgctcat catttttacg ctcgcgttgc tttgcatgtc
     7921 cggctgcgga agccaaacca cgcaggatca agcaaagagc atcgaaaatt atgaatcccg
     7981 cacagactct gccgccccgt taactaaaaa ccaagatgaa accgtaaccg gcataatcaa
     8041 cgctgcggac cgcaaggtcg tgcaaaatgc cgagatcagc ctcaaggtac aagaggttgc
     8101 tgcagcggca gataaaatca ctgacctgaa ccaaaccaac ggaggctaca cagtcaacag
     8161 ccacatctat cgcaacggcg aacatatatc cgcagatctt ttactgaaaa ctccccagca
     8221 gaagctcacc agtttcatcg ccagcattgc agccctcggg gaagtcactg acaaacgtgt
     8281 ttctgccgaa gatgtcactg agcaatatta cgacgccgaa gcccggttaa aggtaatgcg
     8341 ggccaaagaa gaacgactcc tcagtttgct ggacaaggcc aacagcataa cggatattat
     8401 cagcatcgag aaagaactgg gacaaacccg cagcgaaata gaggtgttaa ccggccggct
     8461 gcaatatctg tccaatgcag ttgattacag cgcagttcac atcaatctga tccaagccgt
     8521 ccccggcgca attaaacccc cgcagggaac cataggtaaa tcagtacaag gactgatcac
     8581 cagcctcaac aatatgatcc atttcggcag caatttaatc gttggcctgt tcgtgctgct
     8641 gccctggctg ttgctgatac tcatactggt gctggtattc cgatatttct atcgcagaaa
     8701 gaaaaaataa ccaggctgct tatcccggtt gacgtatgaa ctgacagagg tggaatgatg
     8761 gataaatgtg tcttctgcga ccgcgcagaa atggtactgg aaaaccggct ggcctgggcc
     8821 aaatatgatc aattcccggt ctcaccgggc catctgctga tcatcagcaa acggcatgtg
     8881 ccggatttct ttgctgccac cacagaagaa cgccaggcat tcaatgaatt gctgaacgaa
     8941 gctaaaaata tgctggaaga aaaatatcac cccgacggct acaacatcgg cgtaaactgc
     9001 ggcgagagcg ccggacaaac catcatgcac ctgcacattc atctgattcc aagataccat
     9061 ggggacatgg ataacccccg cggcggcgtg cgcggggtga taccggagaa gcggatttat
     9121 tgaatgggag gattcatccc cctcgtatca agatttacat atttatttta tcttaattaa
     9181 gacatcggca tgaaaaagat gagcgcagtt gaaatggtct tcctcacatg aacccactgc
     9241 ctggcgccct catttcttat aatgaaaaat gcaggacatc attccctttt atcgaattaa
     9301 tgggtatgga tttgttaaaa caaccgccat cattgataac tgggatgggg ggttctgtta
     9361 aatgattcgg gtagaaggtc aaactccata tcaggccgtt gacaccgcca gttttgccga
     9421 agacctattt gtagagctct ttcaggaaac cttcgggctg gacaaaacgc aatacctggt
     9481 taatgaatat ccttgcgaga atatctatgg cgacaattgt tacatcgatt atgccctgaa
     9541 aaccgacctg gaagcttacg ccatcgaaat agacggcgaa cagtggcaca atcccgccct
     9601 ggtctcagtc gacaagtatc tggatgatct tttaaagcgc aacagtctga cctggttagg
     9661 ctggcgggta tacatatgga cgtaccgcca gttggtcaat gaacgcgaaa aagtgaaatc
     9721 ggaattggtc cagtttctgg gccaaagccc ccatttcaag atctatgacg actacctgcc
     9781 ccgccagcgg ggtacggtac tggaactgcg cccccatcag gaagaaaccc tgcagcgatt
     9841 gcaggagctg cgcggcgaat ataacagcat ggcgctcatc gccgatgccc agggaaccgg
     9901 caagacgacc accgcagtcc tggatgcctg ccgattaggg ctgcgcactc tctttgtggc
     9961 tcatactctc gaactgttgc agcaggctgc caggagattc agggaactct ggccgcaggc
    10021 ccgggtaaca attatcgacg actactccgg gcctctggac ggggatgtga tgattgccag
    10081 cattcagggt ctgcataatc gtttgagcca atttgccccc gatcatttcg gttacatcat
    10141 catcgacgaa gctcaccatg ccgcttcccc cagctaccgt caggtgatct cctattttca
    10201 acccatgttt ttgctgggcc tgaccgccac ccccgagcgc catgatcagg aatccatcat
    10261 ggaattgttt aaaaatgaag cccatcgcct ggacctgaaa accgcagtag aaataggaga
    10321 actggtaccc atccgctgtg tccgggtaaa gaccaacata gacttcagca gcgtacgcat
    10381 caacggcatt cgctacaatt accgcgacct cgacacctgt gtccatgttc ctgaacgcaa
    10441 ccggctcatt gttgacacct acctcagcca tgtaccgggc caaaaagcag tggtcttttg
    10501 tgccagtgtc cagcatgccg cagaagtggc ccagctattc caggaatcag gaatcaatgc
    10561 ccaactggtg gaaggacgca tgaaaaaacc gctgcgcgaa gagatcctcg atcaatatcg
    10621 gcaaggcatt gtcaaggtat tgtgtgcctg tgacatcctc aacgaaggct gggacagccc
    10681 ggaaacggct gtactgttca tggccaggcc cacgctgtca aaagtcatct atctgcagca
    10741 gttgggccga ggcacccgcc gcgcccaagg caagaatgcc ctgctggtat ttgactttat
    10801 tgataacacc gttcgccata accattcggt caatctacac cgcctgttcc gctcccgcga
    10861 gtaccggccc ggagcattgg tgatcgcccc acaggagcaa atggaagaag aaaagcgtcg
    10921 cctgctgtcc ggagaaaaaa tggacaccat tctgccccac aatatatttg tccgtgatta
    10981 tgaactggtc gacctttttg actggcagga agaaatcaag catatgatgt ctctgcatca
    11041 gctggccatg gaactgtacg tagatgacgc cacagcccgc cgttgggtag atgaatgcaa
    11101 aatcaccccc ggtcccgacc tggaactgcc catgggcaag gtaacccacc gctatttcag
    11161 ccaggagcgg ctggaggaca tccgcgaaca gctgaacata cctgcccgtg tacccgaagc
    11221 catacgggaa gatttctttc gctacgtaaa ggccggacaa atgagttttt cctataagcc
    11281 ggtcatgtta aaagggatgc tgcgcctggt caatgacaaa ggacaagcag aactgggagc
    11341 cctggtcagc tacttccgca atttctatga acagcgggct gatgccgatc tgcagataga
    11401 agtgtccggc gctgttatca accgggtgaa agaaatgaat gactttgacg ttgcccgcct
    11461 gatgctgacc atgccctttg aaaaatttga acgcaaattc ttcctggaac accgcaaaga
    11521 tttgaatctg gtgggtttcg tgcccgcgct atggaagcgg ctcacggtgg aagataaggc
    11581 ggaattgatt gagatttgtg atcggcagat tgagcggtat tatgcgcggc gggtggggaa
    11641 agaatgagga gtttgaatta taaactatga aaattttgta tcaaaagcgg aatttactgg
    11701 tctcggcggg cttatatccc tagtaataag ttacccggtt aacttcctat attgttatat
    11761 ttaggcattt attctacaat aaagtataaa cactacctaa agggagctat tctatggcat
    11821 tttttgattc cgtaaaagaa gcaataactc aaaaaccagc aaatttaaaa aagccgtttt
    11881 tttataaaaa agatagtgat gcgcagcgtc aactggaaaa actaaaagaa atatataaaa
    11941 ctgctcctaa ctcgattaag ctgaagattg atcaagatat taaaatgctt tcttacggta
    12001 ttgccggaga agaaaatatt gcctttgaat taagcaacag ccttctgccc attattgtgc
    12061 tgcatgattt acatattgaa tttgaggatt tatcaacgca aatcgattat ctgattatca
    12121 ccaacaaatt ttcactgatt atcgaatgta aaaatcttat cggaaatatt gaagtaaaca
    12181 gcaatgggga ttttattcgc acttggcagt ataacggcaa atataataaa gaagggatct
    12241 atagccccat tacccaaaac gcacgccatc tggatatgat caaaagaata aaattaacac
    12301 acaagaacaa ttttgtaacc aaagctttat tcgaaaaata ttttgatgag tttcataaat
    12361 cagtggtagt actagctaac cccaaaactg ttattaatat gaaatatgct aaaaaggatg
    12421 taaaggataa aattattcgc tgtgatcaat taactgaatg tattaaaaaa atgattagtg
    12481 acagtaaaaa tgcgcttttt tcggagaaag aaatgtacga aatagcggat ttctatttaa
    12541 gtttacatac tcccaatact gttgattata caaagaaata ccagatcgac gaacccgcgc
    12601 aggaagaatc cataccaaca gtagcaccaa ctttggcacc tacaactgca actacaactg
    12661 caccgaaatc agttccggtg gcaaatccgg tagcaacacc gacgaaaccc aatatagatg
    12721 aaacaccgtt atataaagca ttgaaacaat accgttatga cactagcaaa gctgaaggaa
    12781 taaagcctta ttttatttat aacaatgccc agatggaaga gctcatttct attatgccta
    12841 aaacatttga agaaataaaa aaagtttccg gttttggaga tgttaagtgt caaaaatacg
    12901 gatcagctat tctggagatc attaatcaat ataaatgagc gaaaccgggt ggaaggtaac
    12961 tgagtgattg gaattatgga tagtgaacag tatatatata aaaaagaggt tgactggtcc
    13021 ttgctacatg aaggatttac gatcccagtt tcctttcaag tcaggtttca agaaattatc
    13081 ggacatggat tagataaagg tcagaccaaa aatattactc tacttgtgga gaataagcca
    13141 ttcaccgcac agttaaaaaa ccaggatttt gaccagtcac gttatccaac tcataaagat
    13201 ctcctacaaa tcagatatac cagaaatagc cttttggcag atattttaag aacaatattt
    13261 aattccagct atgaatactt gaaaataaaa agatcagagt tgggacagaa taatcgtctg
    13321 attaaacttc ctgaaaatat tagggaatat ttggttttat atacaagtga ttctgctgat
    13381 ttatttattg ccgattgttt aaccatctgg gatctacaaa aagaaattca ggttatatcc
    13441 agttatcctg aagaagtact cgaggctgaa attaattata aaaatgttga tccctcagca
    13501 gggcttgatt tgcgagaacg tatcgtcaaa gtcagacgtt ttgataaagc aatcgccgat
    13561 aatttaaaac tattgtatga ttatcgatgc caaatttgtg gcaacaactt ttccgccaaa
    13621 tatgatacgt caattgccga agcccatcat attgattctt ttatcaaatc actcaacaac
    13681 gattcagata acatcatgat tgtttgcccc aatcaccata gaatcataca caaaactatg
    13741 ccaacttttg aaaggtccaa tcgttctttg attttccaga atggttatca ggaaaggttg
    13801 agacttaatt atcatcttta atgcatgctt accccaccac caacttattg gtcttcttca
    13861 aatacttcgc cggtatcggt cgatgcagtt tccagataac attcataggt ctggagcctt
    13921 cgtgtttgac gtaatcagcc agaccgagga atgtataggg tgctgcgccg gcaatatctg
    13981 ttttaaattc acgaacgaac aacagcactt tgctgcctct ctggcgatgg tgaacataac
    14041 gctgtccagt tggggatgtt tctgaggtag tactttggct ctgccagtga aataaccact
    14101 cgttgatgga atagtcatta tacatggtgg ttggtgagta atctttatca gatttattta
    14161 gggtcacaaa gaagatgtca gcgctttttt cgggaaggta cttgacgcct tcacgaactg
    14221 ttgcgggttt catgaaatcc atagccacta gaatctgatc acgggtatag gtacaatgca
    14281 aatccaatgg acaatcaaag ccgagctcta ccggttcgtc gataaaatcg atatggttta
    14341 ggttatattt cagcagctca atcaactctg cacaaagcac cgggttgttt ctgatttgct
    14401 gtaggccttc cttcagactt acaaagccgc attcctccac cggcttttgc caaacggtaa
    14461 actgaaacat ttgcagcatc cgctgttcag attcgcatag cacttcaaaa tccaagccat
    14521 caaggtcgga gaagactttg aatagaaagt tgatccaccg tctggaatca attgcacaaa
    14581 tccgtgaaaa ggctttagtt agtacttcct cctgcggttc aacaaagtca tctctgactg
    14641 cagccattac gcataatctt gaaaaactac cgcatttata aatactgcgc acatccaaat
    14701 gatagtagga tgtaaaattg gctagcgtga gaggcagtcc gctgtcctcg gtaaaggttg
    14761 ccaggcggga taccagacct gccttcacgc cgaatgaact gcggatattg tttaatatgt
    14821 attccttggc tttgcgctct agctggatat agcagccttt gggtagggag gcaaaaccgt
    14881 ctttgatttc acgctgtacg cttttattgg tattagccag cagggctgca aatttgtctt
    14941 caaagttgta ttttctatta gcctgcccga taaaatctaa aaccgttaag cactctttgt
    15001 tatcggccaa gcgaagccca cgccccaact gctgcaggaa aactgtcaaa ctctcggtcg
    15061 gacgcaggaa taaaacggta ttgacctccg gaatatcaac accttcatta taaatatcca
    15121 ccacaaagat aaaccggacc tgtccgctga ccagtttctg cttggcggta tggcgttcct
    15181 catcttgtga tgtccccact agatacatag aagaaatgcc gagtccgttg aaatactctg
    15241 ccatatatcg ggcatgctca accgaaacgc aaaaacccaa gccttttaca tcatttatat
    15301 ctgttacaaa ttttacgagt gaggcaatga tcatgttggc acgtctatca gccgcggcgc
    15361 gattgagggt atagagattg cttaactcgc ttttgtcgta tccaccccgc acccatttta
    15421 actcgtttaa atcgacggtg tcggtcacgc cgaagtactg gaacgggcac aacagcttgc
    15481 ggtcaatggc ttctggcaaa cggatctcag ccgcgatgcg gttgtcaaaa tactccaaga
    15541 tgctcttgcc gtccatgcgc tccggtgtgg cggtaagtcc cagcagaatc ttgggttggt
    15601 aataggcgag cagtttttgg taggttggcg ccgctgcatg atgaaattca tcgacgatga
    15661 tatagtcata gaaatcaggt gatgtcttgg tctgcagctc ttgtgagtta aaagtctgga
    15721 ccgaaataaa taaatgatcc agactgtccg gcttatagtt gccgacaaac aaatccccga
    15781 agttggcatc cttcagcacg ccttgaaaac acaaccagct ctgctttaaa atttcttctc
    15841 ggtgggcgac aaatagcagg cggcatggtt ggcccgaatt ggcttgccgg aagcgcttat
    15901 aatcgaaggc ggaaatgacg gttttgccgg tgccggtggc agcgataatc agatttttat
    15961 agtacccgcg caccttgcgt tcggcctcca acttatccaa tatttcttgt tgataggggt
    16021 atggccgaat atcaaaagta aacaaggtta cgctatcccc gcctgttagt ttttctgctg
    16081 ttaaggcttg ttttaagcgt gtcttctgcg tttcgttata gtcttcaaaa tcggtagagt
    16141 tccaatagct ttcaaaggta gcatcaatct tgcgaatcgt ttccggcaaa tctttggcgg
    16201 taactttgac attccactct agaccgctgg agatggcagc attggataaa ttggatgagc
    16261 caacgtaggc ggtcgtaaat ccggtatctc gataaaacac ataagtcttg gcgtgcagcc
    16321 gggtgcgttt ggtgtcatag gaaactttga tttccgtgtt aggcaggcgg cgcagttctt
    16381 caatggcttt aacgtccgta gcacccatat aggaggtcgt aataatgcgc aatcgtcccc
    16441 cgcgttgagt aaagttgcgc agttcatcaa taatcagacg tagaccactc cacttgataa
    16501 aggataccag aatgtcaatc cggtcagctg aagcgatttc cttttttaac tcggtataca
    16561 tgctgggctc atggattgcc ccggtaaaca aagaactttg tgccaacgat gtctgcggac
    16621 gaatgatctc tactttttca ttcacagcat aaacggtgtt ttgtttgttt aacaacgcta
    16681 gcagttgttc accgcgttca tcgacggata acatgtcaaa gtcgtcttct tgtgtttcgg
    16741 atatgatgac ctggacaatt ttgttggcca ggtcgatttg tctctggata tccccaccct
    16801 tgtccttgac gttgtccatt cctttttcta tgatctcaac catgtatttt gccaaaatcc
    16861 tggaggcttc cgcgctgtca atgggcgcaa ccgattttag cagctcgtca gcctgatcaa
    16921 gctcctgccc caactgcttg ctaatgacct gttcatacaa cccttttttc aactcatcat
    16981 ctcctccatc agcctctcca caatcggata atccgccggc gcccattcaa gtgttttaag
    17041 ctcttgcggt ttaacccatt gatggttgac atgttctctg cgaacgaatt ttttcgtatc
    17101 aacatgacag ataaaactgt gcatggtcag ctcaaaatcc gggtagacat ggttgatggt
    17161 catgaagtaa tctttctcct cgatcgttat ctctatattc atttcttccc ggagttcccg
    17221 catcagggct tccgttctgg tctcccctgc ttcgatcttg ccgccgggaa attcatattt
    17281 ataggaaata tattcttgtt tggcctggcc gcgctgcatg cacaggatct gtccttcatg
    17341 aatgatgatg gcggccacta catcgagatg cttcatttgc ttttgccttt ctttgttatt
    17401 ggaacataca aggggacacg tttttataat ataattccca tttgatttcc ataatcctgc
    17461 aaaatatagt agcctgatgt aaacgaccat atttcggtta ttcaataaag ttcattctgc
    17521 ctaaaaaata tcctgtttgt tctatcaata caaaaaagca gcgacattaa tctgtcactg
    17581 catttcacat aaaataatta tcgttttttt agcttctcaa tttccctccg aattcgaagt
    17641 acatatcccg tattatcggc attctttttt gcgacaaatg tttcacgtga aacattgttg
    17701 cccggcaggc gcaagttggg gacggttcga gaccactgaa aaacacataa tagaaaataa
    17761 caa
//
LOCUS       DAOTHZ010000044        17528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3899_length_17528_cov_7.289475, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000044 DAOTHZ010000000
VERSION     DAOTHZ010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 17528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17528
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3899_length_17528_cov_7.289475"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..4575
                     /locus_tag="P5549_06520"
     CDS             271..4575
                     /locus_tag="P5549_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRW12763.1"
                     /translation="MANVLRMGIDVGSTTVKTVILNEAGSIVYSGYRRHNAETKNSTS
                     ELLRDAFEYVGNAALTVLISGSAGISIAADLEIAFIQEVIACNQAIEAIIPQTDVAIE
                     LGGEDAKITFFRGGLDQRMNGICAGGTGAFIDQMAGLLGTDAAGLNQLAAQHKTLYPI
                     AARCGVFAKTDIQVLLNEGARKEDLAASVFQAVVNQTISGLAAGKPIKGNVALLGGPL
                     HFLPELRKSFIKTLQLHEHEVLFPDHAQFFVAMGAALASQNEPLISLTTIIGRLAQLN
                     KDYASESQHLEAFFADANERQLFIQRHARNRVIRASLSACHGDCYLGIDAGSTTSKAV
                     LIDAEGRLLYSFYTNNHGSSINSAVEMLHDLYAQLPPQAKIANSVVTGYGEGLLKAAL
                     HVDTGEVETIAHYTAAEYFQPGVDLILDIGGQDMKCLKINNGVIDNILLNEACSSGCG
                     SFIESFARSLAIPVDEFARLALEAQSPADLGSRCTVFMNSKVKQAQKDGASVADISAG
                     LAYSVIKNALFKVIKIRNPRDLGEKIVVQGGTFLNDAVLRSFELITEREVIRPDIAGL
                     MGAFGAALLARRSALPGQQSTLLNQQQLAHFHLQTSMSNCAGCANHCKLTINKFPDGK
                     RFISGNRCEKFSDKENTAEPVANLYEYKLQRLLGYEPLPENQAYRGTIGIPMVLNMYE
                     NYPFWATFFAELGFRAVHSPRSSRAIYEMGSATIPSDTACFPAKLAHGHIAALIEQGI
                     RTIFYPSIIHERQEQEQADHHFNCPMVISYPEVLKQNMEIIKDNDVVFIHPFLPYDNK
                     KQLAACLYREFAPWGVKKREVAKAVEKAWREDQKFKQDLRHKGEEVLAQLQASGRKGI
                     VLAGRPYHIDPEINHGIPEIITHYGLAVFTEDSLAHLGDVERPLRVVDQWMYHSRLYS
                     VAHFVCSEPTLELVQLNSFGCGLDAITTDQVQEILQQHGKIYTVLKIDEGANLGAARI
                     RIRSLLAAMKKRDQSGKSLIPQPVYQVRVPFSQEMRSDYTILCPQMAPLHFRFLEPIF
                     RSEGYQVELLPEVDRKAIEEGLKYVNNDGCYPAIVVIGQLLQALQSGKYDLDKTAVAI
                     SQTGGGCRATNYFGLLRKALHEAGYENIPVISANLYGNESNSGFKITASLLKKSITGV
                     VYGDLLMRVLHRVRPYEIIPGSANLLFDRWVDRCIEQLSSGDQRAFERNIHGIVEEFS
                     QLEISRQIKPKVGVVGEILVKYHPAANNDIVRVLEDAGAEAVVPDMLDFFLYSAYDSI
                     YLGRHLTGNRKQEWLGRFIVYYLEKQRKTVKQALENSTRFEPPHSIYHKAVLAREMIS
                     LGHHCGEGWLLTAEMIELIQTGVPNIVCVQPFACLPNHVCGKAMIKPLKEKYPQANIT
                     AIDYDPGASEVNQINRIKLMLSVAFKNLPEQNQENKHGKETDAYMPDDYPSMIHLR"
     gene            complement(4718..5944)
                     /locus_tag="P5549_06525"
     CDS             complement(4718..5944)
                     /locus_tag="P5549_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRW12764.1"
                     /translation="MKEVQINERLPFSQSIPLGLQHLFAMSGATILVPFLTGLSPATA
                     LFCSGLGTIIFLLLTRSRVPAYLGSSFAFIATLTVFVKDQQNLASAMTGVLSVGIAYI
                     LIYLILRMFGTQWIHKIIPPVVAGSVVAIIGLSLTPVAVDMASSNWAVGIFTLAVAIL
                     ISIFGKGFSKIIPILLAIVAGYILAWIMGLVDTAMIKASFDTLFILPFSSFGTFNLDF
                     VAILAFAPIALVTLIEDLGHMMILGNITRTDIIENDPGFDKVVLGNGLATGAASLFGG
                     VPLTTYAENIGVLAITKVYSSINLWIAAIAAILLSTFNPLQALIMSIPTSVMGGVSIL
                     LFGMIGAAGLRTLIEAKVDFSQNKNLIIASIIFAFGIGLSSHGVMVATLAGIFLNLIL
                     KEEAPDDGKKADEKAA"
     gene            complement(6086..6724)
                     /gene="upp"
                     /locus_tag="P5549_06530"
     CDS             complement(6086..6724)
                     /gene="upp"
                     /locus_tag="P5549_06530"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRW12765.1"
                     /translation="MSSVNLIRHPLAGNCLRTLRDRNTKTEDFRQAMDKLGLLLAVEA
                     SLNLPTEIDTVITPLDIEARCSYVDDSKILLIPILRAGLGFVDSFVDVLSAAKVAHIG
                     IARDHETLEARAYLDTVPEHPEDFDHVFILDPMLATGNSSVRAIELILGKGYTAGKIT
                     FVCALSTMVGIQQLQSKFPELSIITAAIDPELNEKAYIVPGLGDAGDRLNLL"
     gene            complement(6902..7060)
                     /locus_tag="P5549_06535"
     CDS             complement(6902..7060)
                     /locus_tag="P5549_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12766.1"
                     /translation="MVTKLFMDLKKIFADLASTLILGKNKDAAGIALLLSEKSKALAD
                     ELAGAAHE"
     gene            complement(7168..8022)
                     /locus_tag="P5549_06540"
     CDS             complement(7168..8022)
                     /locus_tag="P5549_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRW12767.1"
                     /translation="MSEKCNTCSSAGNCSVDPEQCEKGTETLLGSLNRIKNVIVVMSG
                     KGGVGKSSVTALLACELNQAGHKVGILDADITGPSQPKAFGIKNERGLKATDYGIIPP
                     VSTNGIKVISINFFLPNEDDPVVWRGPMLAGAVKQFWQEVDWRDLDYLIVDLPPGTGD
                     VPLTVMQTLPVSGIIIVSSPQDLAFMVVKKTIKMAQKMNTSILGLVENMSYAICPHCQ
                     EKIQMFGEPQGKRVAEETQIDYLGELPWDAALNTLVDQGLIEQYESKGMSAIAAQIME
                     KLPVEKLS"
     gene            complement(8136..8573)
                     /locus_tag="P5549_06545"
     CDS             complement(8136..8573)
                     /locus_tag="P5549_06545"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRW12768.1"
                     /translation="MKIIVDADACPVKKIIEKTAAKYHLEVIMVSNYHHQIDSTYATV
                     MIADGQSQAADVMIANQTRAGDIVVTQDYGLAAIILGRHAYALHPQGHRFTDANIEGL
                     LMKRYENQKARQAGKRIAGPRKRTQQDDQHFAQQLEELIQALR"
     gene            8759..9352
                     /locus_tag="P5549_06550"
     CDS             8759..9352
                     /locus_tag="P5549_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRW12769.1"
                     /translation="MLSDEKLALQILSGDMAAFEELMNRYKRSIFTIAYRMMGNYHEA
                     EDISQEVFVTVYNKLYQYDSTKKFGPWIHRIAVNTCISTLRKRNRVVSISFDETYVPA
                     NELDLHDLYEDPQVLIEQQELQAEIEEALLELPESYRMVLILRYQLDLKNQEIAEIIG
                     VSRENVDVKIHRARKALRKILLQRWHEGGLKNELPAL"
     gene            9333..10448
                     /locus_tag="P5549_06555"
     CDS             9333..10448
                     /locus_tag="P5549_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRW12770.1"
                     /translation="MNCQHYREMMFADFETELSPTDQNDLLQHLQTCESCRRHYDLTL
                     RENEVLMDTSNIPAIDAGFNRKVLAQLQAASTNGQTLPRRRSRHLPAYSKAVAAAVLL
                     LACLYVPGILPRLQDDRSIEQTAMVDHSQPSQKSLHNVRAGSEPTQAVEMGLSEQKPD
                     ESVSESSSQVNRESPQYPNHDEVNGAKNADTGTVLKAKHDTAEQTPELFMQTAAEPLA
                     PHDGQGVEQSKGMEASRSLISSLRSLGTPVKPSINNTPAGFVLVETREINEVQTEYHF
                     TDPNREKSFVIKLSPADAAAAGSIPAQEAEMYNDKETMNADMASIAVMPNEASKIMEY
                     NGLSYQLQITSAVFTQEELAAILTTIHLDSPAPSLQP"
     gene            complement(10486..12516)
                     /gene="fusA"
                     /locus_tag="P5549_06560"
     CDS             complement(10486..12516)
                     /gene="fusA"
                     /locus_tag="P5549_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRW12771.1"
                     /translation="MLSKLRNIGIIAHIDAGKTTTTERILYYTGLTHKIGETHDGESV
                     MDYLEVEKERGITVTSAATKCVWKNCDINIIDTPGHVDFTAEVERSLRILDGAVVIFC
                     GKGGVEPQSETVWRQADKYHVPRLAYVNKMDAIGADFFMVVEQIKNRLGAKPLILTLP
                     VFEDESFAGIIDLVSMKYQSFTGGLGDEITTTAIPEEYQAEADQWRLVLLEKIAENSI
                     TLMELYYNDEVIPDDLLLNEVRNSTIAGNIVPVICGSSYRNIGVQTLLDAIVNFLPSP
                     LDIEAAQAIDLTTDQEFTISPDNKEFFSALIFKIVSDRHVGKLAFARIYSGSIKAGSY
                     VYNSSKQKKERIGRLVKMHANHREEIEEVNAGDIVAFIGLKDVGTGDTICHEDHPVLL
                     ESIEFPEPVIQIAIEPRTQSDQNKISDALAKISAEDPTFKISYNKDTGQTLLAGMGEL
                     HLEVISERLAKEFKIDINIGQPQVAYKETITRAAKHETRYVKQTGGKGQFAHLVLQLE
                     PAEGFEFVNKIVGGVIPREYIPAVEAGVKQALQEGPLKGYPVVNVKATLSDGSYHEVD
                     SSEMAFRVAAMQAAKDCMRKAAPQLLEPIMRVEISSPDEYTGNIIANISNRRGRMDAM
                     EMQGSTQVIRAFVPLAEMFGYSTSLRSITQGRAAFSMEFSHYEERSITEKDK"
     gene            complement(12726..13511)
                     /locus_tag="P5549_06565"
     CDS             complement(12726..13511)
                     /locus_tag="P5549_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12772.1"
                     /translation="MSYGKHFNYTKEGDVAIVTLCNPKGFNLVAGETFFRELYEIQEI
                     MEEDKTLRACVINAEGDHFTAGVDLKTLRQADSQWIMDRLVWLQRLYSRWQEYPFPVI
                     AAIQGICYGSGMELILGCDFRIAADNVRFSIPEVRFGLSPDMGGTAKLTHLVGIGQAK
                     RLILGCDEINAEEALRIGLVEIVVKSEELQQTALKYAKRMAGMPPAALRFAKKGINVA
                     NESSVAASLLFEEAQSTFCCGTQDQNEGIDAFFEKRKPVFHNK"
     gene            complement(13608..14150)
                     /locus_tag="P5549_06570"
     CDS             complement(13608..14150)
                     /locus_tag="P5549_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HRW12773.1"
                     /translation="MNKYHIDKEDAVLVIIDIQERLMSAMPERERVYKNTALLIETAK
                     QLGIPMVVSEQYPRGLGPTAAEIKDHLADYQYIEKVSFSVSEQLLEILPKDRKTLIVT
                     GSETHVCVFQTVRDMVEAGYTVHLPQDAVCSRFAINYCSGLDLMRDLGAVITNSETIV
                     FDLLKVAGTPEFKAISPLLK"
     gene            14288..15424
                     /locus_tag="P5549_06575"
     CDS             14288..15424
                     /locus_tag="P5549_06575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRW12774.1"
                     /translation="MRNFRTIIGFGLILFITITMVIISQPAIKATRSGHEPVKTSETI
                     RLLATGDILMHNTVIAAGLNHGEYQYEQMFAPIKHIVESADYAMINLEAALAGPKNGY
                     TGYPLFNAPDSLATALNNTGFDLVTNANNHILDRGYEGAIRTLDVLRQSGLDTLGVNP
                     SQQERETPLIKDIRGVKVGFLAYGYDTNGIPLPQDKPYFYNLLDEQVVLQDITALRPQ
                     VDILVVSLHWGVEYSPYPTEKQIAMAQRFFIQGADVIFGGHPHVLQPAEVLTVSGQKK
                     FIVYSMGNCLGDQNGVERNSGVIISLAFKKDLLTGKTELQSFDYIPTFSHSYIENNRE
                     NFRVIPVPETIQKVKAHQDPHLQDDSLALLEHISQACQAKMSSP"
     gene            complement(15428..16543)
                     /locus_tag="P5549_06580"
     CDS             complement(15428..16543)
                     /locus_tag="P5549_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW12775.1"
                     /translation="MNAAPLTLNQLPYLDSIIHRIVGNMLLISQVSLEGEYQYLGNTH
                     QTLLGYEPESLLHQSIFDYIHPEELFMVRSLFKLGLDTGQFKTTRIRFRCRDEQYIHL
                     ETQGMAVYNKEQQITGAVFVTRDITEEIRLENELARLEQLKTIGQMTAGLVHEVRNQI
                     TSVRGFLQFLGDHEDLQPYGNYFALMIEELDGANQLMADYLSMAKEKEVRFSWQDLNA
                     VIRTLYPFIAVEAARNNHRIILDLGNINEVYMDGNQIRQLIVNLVNNGLEAMQPGFTL
                     TIRTLSQGNHIILAIEDQGAGIDAAIMERIGTPFFTTKEQGTGLGLSICKNIAARHKA
                     KLTIESGTAGSIVSVCFKDFKVLNDHDGLGTQSMFER"
     gene            16785..17282
                     /locus_tag="P5549_06585"
     CDS             16785..17282
                     /locus_tag="P5549_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRW12776.1"
                     /translation="MSTNELNILKKAILIEAEGENFYQLAADQSNNQEVKNAFLYLAE
                     EEKQHGTWLRQLMQSLAPGNSFDLKQLESQAEMSLPPIFDFSQLKGQQKNTLEISVFH
                     IGILMEKASLDYYREAAEKSTNPEAQKLYETLSKWEMHHLNELEKVYDGLSEDWFDHQ
                     GFSPA"
     gene            17397..17472
                     /locus_tag="P5549_06590"
     tRNA            17397..17472
                     /locus_tag="P5549_06590"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17430..17432,aa:Ala,seq:cgc)
BASE COUNT         4692 a         4325 c         4010 g         4501 t
ORIGIN      
        1 aaccttctga ttaagagtca gctgctctgc ctgattgagc taatggcccg gatgttttgc
       61 cgcaagtatg attataaacg ctgttatttc agaaatcaag agcaaagcat taacttcaat
      121 tcaacttcaa ccaagacgtc aaccaagatt catcacagtt gcttgattct tacattcaac
      181 acatgacaaa acccgccatt taatctacta ttaaagcata gtattatgat gaacaattat
      241 ctgggtttcg ggagaggagc aactatcagc atggccaatg tcttgcgcat gggaatagat
      301 gtgggatcaa ccacggttaa aactgtgatt ttaaacgaag caggttctat cgtatacagc
      361 ggctatcgtc gtcacaatgc cgaaaccaag aacagtacca gcgaactgtt aagagatgct
      421 tttgaatatg taggcaatgc ggcactcact gtgcttatca gcggttcagc aggtatttcc
      481 atcgccgctg atctggaaat tgcctttatc caggaagtca ttgcctgcaa tcaagccatt
      541 gaagccatta tcccccagac agatgtcgcc atagaattag ggggcgaaga tgccaagatt
      601 acttttttcc ggggtggact cgatcaacga atgaacggga tttgtgccgg aggtacagga
      661 gcctttatcg atcaaatggc cggtctcctg gggactgacg cagcggggct gaatcaactg
      721 gcagcacagc acaaaaccct ttacccaatt gccgcccgtt gcggtgtgtt tgctaaaacc
      781 gatatccaag tgctgctcaa tgaaggcgca cgcaaagaag atttggctgc ctccgtgttt
      841 caagctgttg tcaatcagac catcagcgga ctggcggccg gcaagccgat taaaggaaat
      901 gtcgccttgc tgggcggacc attgcatttc cttccggaac tgcgcaaaag ctttataaaa
      961 acgctgcagc ttcatgagca tgaagtcctt tttcctgatc atgcgcagtt ttttgttgcc
     1021 atgggtgccg ccctggcctc gcaaaatgaa ccgcttatca gcctgaccac gatcatcggg
     1081 cggctcgcac aactgaacaa ggattatgcc agtgaatcac aacatctgga agctttcttc
     1141 gcagatgcaa acgaacggca gctttttatt cagcgccatg cccgcaaccg ggtaattcga
     1201 gcttctctgt cagcctgtca cggcgattgc tatctgggaa tcgatgcagg ttcaaccacc
     1261 agcaaggctg tcttgattga tgctgaagga cgcttgttgt attcctttta taccaacaac
     1321 cacggcagtt ccatcaattc agccgtagaa atgctgcatg atttgtatgc ccaactgcct
     1381 ccccaggcaa agatcgccaa ttctgttgta accggctatg gtgaaggtct gctgaaggca
     1441 gccttgcatg ttgataccgg tgaggtggag accatcgctc attatacagc cgcagagtat
     1501 tttcagccgg gagtcgatct gattctggat atcggcggac aggacatgaa atgtttgaaa
     1561 atcaacaacg gggtgattga caacattttg ctcaatgaag cctgttcttc cggttgtggc
     1621 tcttttatcg agagctttgc ccgttcgctg gccataccgg tcgatgaatt tgcccgcctg
     1681 gctttagaag cacaatcccc tgccgattta ggatcacgct gtacggtttt tatgaattcc
     1741 aaagtgaaac aagcccagaa agatggggct tcagttgccg atatatcggc cgggctggcc
     1801 tattcggtca tcaaaaatgc gctgtttaaa gtgattaaaa taagaaatcc ccgtgattta
     1861 ggcgaaaaga tcgtggtgca gggcgggacg ttcttaaacg atgccgtact gagaagtttt
     1921 gaactgatta ctgagcgcga ggttatccgg cctgatattg ccggactgat gggcgccttc
     1981 ggagccgctt tgctggcgcg gcgaagtgcc ttgccaggac agcaaagcac cctgctcaat
     2041 cagcaacaac ttgcacattt tcatctgcaa accagcatga gcaattgtgc aggctgtgcc
     2101 aaccactgta agctgacgat taataaattt cctgacggta aaagatttat ctccggtaat
     2161 cgctgcgaaa aattcagtga caaagagaat accgctgaac ctgttgccaa tttatatgaa
     2221 tataaactgc agcgtttgct gggttatgaa ccgttgccgg aaaaccaggc gtatcgtggt
     2281 acaattggta tccccatggt cctcaacatg tatgaaaact atcccttttg ggcaaccttt
     2341 tttgccgaac tcggttttcg tgccgtgcac tcccctcgct cgagcagagc gatatatgaa
     2401 atgggaagtg ccactatacc gtctgacaca gcttgttttc cggccaagct tgcacacgga
     2461 cacattgccg ctttaatcga acagggcatc cgcaccatct tctacccttc gatcatccat
     2521 gaaaggcagg agcaagagca ggcggaccac cattttaatt gtccaatggt catctcttat
     2581 ccggaagtgc tcaaacaaaa catggaaatt atcaaagata acgatgtggt cttcatccac
     2641 ccgtttcttc cctatgacaa caagaagcaa ctggctgcct gtctctaccg ggaattcgcc
     2701 ccctgggggg taaaaaagcg agaggttgcc aaggcggtcg agaaagcctg gcgggaggat
     2761 caaaaattca aacaggatct gcgccacaaa ggagaagaag tactcgccca attgcaagct
     2821 tccggccgta aaggaatcgt cctggcaggg cgcccgtatc atattgaccc ggagatcaat
     2881 catggcattc cggagattat tacccattat ggtctggcag tttttaccga ggattcactc
     2941 gctcatctgg gcgatgtgga gcgtcccttg cgggtggtag atcaatggat gtaccattcc
     3001 cgactttata gcgtcgctca ttttgtttgt tcagaaccga cactggaatt ggtgcagctt
     3061 aattcgtttg gctgcggttt ggatgcgata accactgatc aggttcagga aatcctgcag
     3121 cagcacggca aaatatatac ggtactaaaa attgacgagg gagccaattt gggtgctgcc
     3181 cgcatccgca ttcgttccct gctggcggcg atgaagaaaa gggatcaaag cggcaagtct
     3241 ttaatcccgc agccggttta ccaggttcgg gtgccttttt cccaggaaat gcgcagcgac
     3301 tatacaattc tctgtccgca aatggcaccg ctgcattttc ggtttctcga acccatcttt
     3361 cgttctgaag gatatcaagt ggaactgttg cctgaggtgg atagaaaggc cattgaggaa
     3421 ggattaaagt atgtcaacaa tgatggatgt tatccggcca tcgttgtcat cgggcagctg
     3481 ctgcaagctt tacaatccgg caaatatgat ctggataaaa ccgccgtagc gatttcccaa
     3541 accggcggag gctgccgggc taccaattac tttggcttgc tgcgcaaggc actgcatgaa
     3601 gcagggtatg aaaatattcc cgtcatctct gccaatctgt atggcaatga aagcaacagc
     3661 ggctttaaaa tcactgccag tctgttgaaa aaatccatca ccggagtcgt ctatggggat
     3721 cttctcatgc gcgtgttgca tcgggtgcga ccctatgaaa tcatacccgg ctctgccaat
     3781 ttgctgtttg accgttgggt cgatcgctgt atcgaacagt tgtccagcgg agatcagcgt
     3841 gccttcgaac gaaatattca cgggattgtg gaagagttct cgcagctgga aatatcccgg
     3901 caaatcaaac ccaaagtcgg agtggtggga gaaattctgg tgaaatatca tcctgccgcc
     3961 aacaatgata ttgtgcgagt actggaagat gccggagcgg aagcagtggt tccggatatg
     4021 ttggacttct tcctttactc cgcttacgac agtatctatt tgggccgaca tctgaccggc
     4081 aaccgcaaac aggagtggct gggcaggttt atcgtttatt atctggagaa acagcgtaaa
     4141 accgtcaaac aagccctgga gaacagtacc cgttttgaac cgccccattc catctatcac
     4201 aaggctgtgt tggcccggga aatgatctcc ctcgggcatc attgcggcga agggtggctg
     4261 ctgacggcag agatgattga attgattcag accggcgttc ccaatattgt ctgtgtgcag
     4321 ccgtttgcct gtctccccaa tcacgtctgc ggcaaggcca tgatcaaacc attaaaggaa
     4381 aaatatccgc aagccaatat aactgccatt gattatgacc ccggggccag tgaggtaaac
     4441 caaatcaacc gcatcaaact gatgttgtca gtagccttca agaatttgcc ggagcagaac
     4501 caggaaaaca aacacggcaa agaaacggat gcctacatgc ctgacgatta tccgtcgatg
     4561 attcatctgc gctgagcagc agcagatgct gccggcagca acctgcgaga agcaacagga
     4621 agcccatgat acaaaatccc gcccggggta cttgcatcat gggcttttcg tactctacaa
     4681 accacttcgc tgctgcatca gtttcgtttt aatatcatca ggcagccttt tcatcggcct
     4741 tcttcccgtc atcgggcgcc tcttccttga ggatcaggtt caggaaaatc ccggccaggg
     4801 tggccaccat aacgccatga ctgctcagac caatcccaaa cgcaaagata atcgaagcaa
     4861 tgatcaggtt tttgttttgg gaaaagtcaa ccttggcttc gatcaaggta cgcagtccgg
     4921 ccgcaccaat catcccaaac aggaggatgc tgacgccgcc catgaccgag gtaggaatgg
     4981 acatgattaa cgcctgcaaa ggattaaaag tactcaagag aatggccgcg atggctgcaa
     5041 tccacaggtt aatcgagctg tacaccttgg tgattgccaa aacgccaatg ttttcagcat
     5101 aggtagtcag cggcacgccg ccaaacaaac tggccgctcc agttgccagt ccgttaccca
     5161 gcacaacttt gtcaaacccc gggtcatttt ctatgatgtc agtgcgggtg atattcccca
     5221 gaatcatcat atggcccaag tcttcaatca atgttaccaa ggcaatcggg gcaaaagcaa
     5281 gaatcgctac aaagtccagg ttaaacgtac caaagctgct gaaaggcagg ataaacaagg
     5341 tatcaaagga ggcctttatc atggcggtat caaccagccc cattatccag gcaagtatgt
     5401 atccggcgac aatcgccagg agaatgggaa tgattttgga gaagcctttg ccaaatatac
     5461 tgatgagaat ggcgactgcc aaggtgaaaa ttcccaccgc ccagtttgaa ctggccatat
     5521 caaccgctac cggggttaaa ctcaatccga taatggcgac cacactgccg gccactaccg
     5581 gaggaatgat tttatgaatc cactgggtac caaacatgcg aagaatcaag tatatcagaa
     5641 tataggcgat accaaccgac aaaacgccgg tcatggccga agccaggttt tgctgatcct
     5701 tgacaaatac agttaacgtt gcgataaagg caaatgaaga acccagatag gcaggcaccc
     5761 gactgcgggt cagcaacaag aagatgatgg tgcccaaccc tgagcagaac aacgcggtcg
     5821 caggactgag tccggtcaga aaaggcacca ggatagtggc tccggacatg gcgaataaat
     5881 gctggaggcc caggggtatc gattgggaaa atgggagtct ttcattgatc tgtacttctt
     5941 tcaaaggttc atcctcctca tattaaatat ttattatcga atcggttaac gcaggccgca
     6001 tcaactgctt gtgtcgccgc agcaaggttg agacgttgaa gcatctgtcc caactgtcgt
     6061 tatcccgacg attatcccgc cggccttaga gtaaattcaa gcggtctccg gcatcgccca
     6121 acccaggaac gatatacgct ttttcgttta attcaggatc aatggcagcg gtaatgatgg
     6181 atagctccgg gaatttgctt tgcaattgct gaatgccgac catggttgaa agggcacaga
     6241 caaaggtaat tttgcccgcc gtatagccct tgccgagaat aagttcgatc gcccggacac
     6301 tgctgttgcc ggtggccagc atgggatcaa ggatgaacac atggtcaaaa tcttcgggat
     6361 gctcgggaac cgtatcaaga taggcgcggg cttccaatgt ttcatgatcg cgggcaatac
     6421 cgatatgagc gaccttggca gcggacaaaa cgtctacaaa gctgtccaca aagcccaacc
     6481 cggcccgcaa aataggtatc agcaaaattt tgctgtcatc aacatatgaa caacgtgcct
     6541 caatatccaa gggagtaata accgtgtcaa tttccgtagg cagatttaga cttgcctcaa
     6601 cggcgagcaa caatcctaac ttgtccatgg cctgacgaaa atcttcggtt ttggtgttcc
     6661 ggtcacgcaa cgtacgcaga caattcccgg ccaaaggatg ccggatcaag tttactgaac
     6721 tcaatttata ccctccttat aaagtcttgc atgaaaacca agcctgctgt gtcttgtcca
     6781 atcacctcca ccatgttttc tttttggatt tgctgcagta gccgtctctt gttttcatga
     6841 catttcacaa aaaaacactg cccttttcag gcagtgctat tattaacgtt taaaacagaa
     6901 gctattcatg ggctgctcca gccaattcat ccgccaaagc cttgcttttt tcggacaaca
     6961 ataaagcgat tccagcagcg tccttattct tgcccaagat aagggtggaa gccagatcag
     7021 caaatatctt ttttaaatcc atgaataatt tggttaccat actgttgccc cctttgcttg
     7081 caatcgccct tcactgaatt gtctggggtt ttcttcttta aattataatc atattttatg
     7141 catccgtaaa tggcaggctg caaaatatca ggacaatttt tcgaccggca atttttccat
     7201 gatttgggca gcgatagcac tcatcccttt tgattcatat tgttcaatca aaccctggtc
     7261 aaccagtgta ttcaaagcag catcccaagg cagttcaccc agataatcaa tctgggtctc
     7321 ctccgcgacg cgtttgcctt gtggctcccc aaacatctgg atcttttcct ggcagtgcgg
     7381 acagattgca taactcatgt tctcgaccag tcccaggatc gaagtattca ttttttgcgc
     7441 catttttatg gtcttcttga cgaccataaa agccagatcc tgcggtgaag atacaatgat
     7501 gattccgctg actggcaacg tctgcataac ggttaaaggt acatccccgg ttcccggggg
     7561 cagatctacg attaaatagt ccagatcgcg ccagtcaact tcctgccaaa actgtttgac
     7621 agccccagcc agcatgggtc cccgccaaac aaccggatca tcttcattgg gcagaaaaaa
     7681 gttaatggaa atcactttaa tgccattcgt gctgacaggc ggaatgatcc cgtaatcggt
     7741 ggctttcagt cccctttcat tcttgatgcc aaaggccttg ggctgactgg gcccggtaat
     7801 atcagcatcc aaaataccca ctttgtgacc cgcctggttt aattcacagg ccaaaagcgc
     7861 ggtaaccgaa gatttgccga ccccgccttt gccgctcatg acaacgatga cattttttat
     7921 ccggttaaga gaacccagca aggtttcggt ccctttttca cattgttcag gatcaactga
     7981 acaatttcca gcagacgaac aagtattgca tttttctgac actttgtaac ccctttccca
     8041 tgtaccatgc ttaatgaaat aatgttgtcg tatttgacca tatttattgt atagcaatta
     8101 ccgcaatatg caaatggtca tttgccgaga accctttatc gcaaagcctg aatcagttct
     8161 tccaactgtt gggcaaaatg ttgatcgtcc tgttgggtac gcttgcgagg tccggcaatg
     8221 cgttttccgg cttgccgggc tttttgattt tcatagcgtt tcattaacaa accctcaata
     8281 ttggcatcgg taaagcgatg gccctgggga tgaagggcat aggcatgccg gcctaagatg
     8341 atcgctgcca gtccatagtc ctgggtcacc acaatatccc ccgcccgggt ttgattggca
     8401 atcatcacat cggccgcctg ggattgccca tcagcaatca tgaccgttgc ataggtgctg
     8461 tcaatctgat gatggtagtt tgaaaccatg atgacttcaa gatggtattt ggcagctgtc
     8521 ttttcgatga tttttttcac cggacaggca tctgcatcaa caattatttt caaagcatga
     8581 tccgcctttt ttaatggtgt tttattccag gaatatatta tttgggaaga tttttgataa
     8641 tagtctatat cgctgctcgt ttttcaggta tcatatttct atagctatta tatccaagtg
     8701 taagccgctc gactacaatc ccgtattata tacgggcagg accgggagga cgaaaatctt
     8761 gctcagtgat gaaaaattag ctctgcaaat cttatccggt gatatggccg cttttgaaga
     8821 gctgatgaac cgatataaaa gatcgatttt caccatcgcc tatcgcatga tgggcaatta
     8881 ccatgaagct gaagatattt cccaggaagt gtttgtcact gtctacaata aattgtacca
     8941 gtatgacagc accaaaaaat tcggcccatg gatccatcgg atagccgtca atacgtgcat
     9001 cagtaccttg cggaaaagaa accgggtagt ttcgatcagc tttgacgaaa cctatgtgcc
     9061 ggccaatgaa ttggatttac atgatctcta tgaagatccg caggtcctga tcgaacaaca
     9121 ggaattgcaa gccgaaatcg aagaagctct gctggagctg ccggaatcct accggatggt
     9181 cttgattctg cgttatcaac tggatttaaa aaatcaggaa atcgctgaaa tcattggtgt
     9241 gagccgggaa aacgtggatg tcaaaatcca ccgtgcccgc aaggctttac gaaaaatact
     9301 tctgcaaaga tggcatgaag gggggctcaa aaatgaactg ccagcactat agagagatga
     9361 tgttcgctga ttttgagacc gaactttctc caacagacca aaatgatctt ctgcaacatc
     9421 tgcagacttg cgagtcctgt aggcgtcact atgatttaac cttgcgggaa aatgaagttt
     9481 taatggatac cagtaatatt cctgccatcg atgcaggttt taaccgcaag gtattggccc
     9541 aacttcaggc agcatcgacc aatgggcaga cactgcccag gcgacgcagt agacatctgc
     9601 cggcttactc caaagccgtc gccgccgctg tgctgttgct ggcctgcctg tatgtaccag
     9661 gtattttgcc ccggctgcag gatgaccggt cgatcgaaca aacggcgatg gtcgatcata
     9721 gccagccctc ccaaaagtct ttgcacaacg tgagagcagg aagcgagccc acccaagcag
     9781 ttgagatggg attgagtgag caaaaaccag atgaatccgt ttcagaatca tcgtcacaag
     9841 taaatcgaga gtcaccgcaa taccccaatc atgatgaggt caacggagca aaaaacgcag
     9901 acaccggcac ggtgttgaaa gcaaagcatg atactgctga acaaacgccc gaactgttca
     9961 tgcaaacagc cgccgaaccg cttgcgcccc atgacgggca aggcgtcgaa caaagcaaag
    10021 gaatggaagc cagccgctcc ctgatttcca gcttgcgcag cctaggtact ccggtgaaac
    10081 ccagcatcaa taatacgccc gcaggatttg tgctggtgga aaccagggag atcaatgaag
    10141 tgcaaaccga atatcatttt accgatccca accgtgagaa aagctttgtg atcaagcttt
    10201 ctcccgctga tgccgcggct gccggttcca tccctgcaca ggaggccgaa atgtacaatg
    10261 acaaggaaac aatgaacgct gacatggcca gtatcgctgt catgcccaat gaagccagca
    10321 agattatgga gtacaacggt ctgtcttatc aactgcagat aaccagtgct gttttcaccc
    10381 aggaagaact tgcagccatt ctcacaacca tccatctgga ctcgccagcc ccatctcttc
    10441 agccataaaa aaagacttcg tgccgggcac gaagtctttt tattattatt tatctttttc
    10501 cgttatactg cgttcttcat aatgggagaa ttccattgaa aaagcagccc ttccctgggt
    10561 aatggagcgc agcgaggtgg agtaaccaaa catctccgcc agcggcacaa aggcacgtat
    10621 aacctgggtg ctgccctgca tttccatggc atccatccgc ccccggcggt tgctgatgtt
    10681 ggcgatgata ttgccggtat attcatccgg tgaactgatt tccacccgca tgatcggctc
    10741 cagaagctgt ggcgcggctt tgcgcataca atccttggca gcctgcatgg ctgcgacccg
    10801 gaaggccatt tccgatgaat ccacttcatg ataggaaccg tccgacaatg tggccttgac
    10861 attgacgacc ggataccctt tcagcggccc ctcttgcaaa gcctgcttga cgcctgcctc
    10921 cactgcggga atatactccc ggggaatgac gccgcccacg atcttgttga caaattcaaa
    10981 tccttccgcc ggttcaagtt gcaggaccag atgagcaaac tggcccttgc cgccggtttg
    11041 tttcacatag cgggtctcat gcttcgcagc tctggtaata gtctctttgt aagcgacctg
    11101 cggctgacca atgttgatat cgattttaaa ctccttggcc aaacgctcgc tgatgacctc
    11161 caaatgcagt tctcccatcc cggccagcaa ggtttgtccg gtatccttgt tatacgaaat
    11221 cttaaaagtc ggatcctcgg cagatatttt tgccagggca tcagagattt tattttgatc
    11281 agactgggta cgtggttcaa tcgcaatctg aatgaccggt tccggaaatt cgattgactc
    11341 cagtaggacg ggatggtctt catgacagat ggtatcacct gtgcccacat cttttaaacc
    11401 aataaaggct acaatatccc cggcatttac ctcttcaatc tcttcccgat gattggcatg
    11461 cattttgacc aagcggccaa tccgttcctt tttttgcttg ctgctgttgt acacataact
    11521 gccggctttg atggagccgg aataaatccg ggcaaaagcc agtttgccga cgtgccggtc
    11581 gctgacaatc ttaaagatca gggcagaaaa gaattcttta ttgtcaggac tgatggtgaa
    11641 ttcctgatca gtcgtcaaat caatggcctg ggcagcttca atgtcaagcg gggacggcaa
    11701 aaagttcaca atggcatcca acagggtttg cacgccaata tttctgtagg aactgccaca
    11761 gatgaccggt acgatatttc cggcaattgt gctgttgcgg acttcattga gcaataaatc
    11821 atccgggatg acttcatcgt tataatagag ttccatcagg gtaatgctgt tctccgcgat
    11881 cttctccagt aaaaccaaac gccattggtc agcttccgcc tgatattctt ccggaattgc
    11941 agtcgtcgta atttcatctc ccagaccgcc ggtaaaggat tgatacttca tggaaaccaa
    12001 atcaataatc ccggcaaagg attcatcctc aaaaaccggc agcgtcagga tcaacggttt
    12061 ggcacccagc cggtttttga tctgctccac caccataaag aaatcagcac cgatcgcatc
    12121 cattttattt acataagcca gacgcgggac atgatattta tcagcctgtc gccagacggt
    12181 ttccgactgc ggttcaactc cgccttttcc gcaaaagata actaccgcac cgtcaaggat
    12241 gcgcaaactg cgttcaacct cagcagtaaa gtcaacgtgg ccgggcgtat cgataatgtt
    12301 gatatcacaa tttttccaga cgcacttggt ggcggctgac gttaccgtaa tcccgcgttc
    12361 cttttccacc tccagataat ccatgacact ttcgccgtca tgcgtctccc cgattttatg
    12421 ggtcagcccg gtataataga gaattctttc ggtggttgtg gtcttgccgg catcaatatg
    12481 agcaatgatg ccaatatttc ttaatttgct gagcatggaa acaattcact cctcctgttt
    12541 ttaaaaaaac aaacggcatt actattatga tagtagtgcc gaaatacaat cgtttttatt
    12601 aacgcacctg acatctaata atattcactt tccggttatt tgtcaaatgg attctgcaat
    12661 tgatttagaa atataaaaag ccgtatctga gattcccaga tacggctttg cttgcgtatg
    12721 ccagcttatt tattatggaa taccggtttt ctcttttcaa agaaagcatc aatgccttcg
    12781 ttttggtctt gggtcccgca gcagaaagtg gactgggctt cttcaaacag cagactggca
    12841 gccacactgc tctcattggc aacattaatc cctttcttgg caaaacgcag ggcggccggc
    12901 ggcatgcctg ccatcctttt ggcatatttc agggcggttt gctgcagctc ttccgatttg
    12961 acaacgattt ctaccaaacc aatgcgcagt gcttcttctg cattgatctc gtcacaaccc
    13021 agaatcaagc gtttggcctg cccgatgccg accaaatgag tcagcttggc ggtcccaccc
    13081 atatcgggag ataatccgaa gcgtacttcg gggatggaga accggacgtt gtccgccgca
    13141 atcctgaaat cgcagcccaa aatcagttcc atgccggagc cgtagcaaat tccttgaatg
    13201 gcagcgatga ccgggaaggg atactcctgc cagcggctgt acagtctctg cagccatacc
    13261 agacgatcca tgatccattg ggaatcagcc tgccgcaggg ttttcagatc gacgccggcg
    13321 gtgaaatgat ccccctcggc attgatgaca caagcacgca aggttttgtc ttcctccata
    13381 atttcctgaa tctcatacaa ttcccggaaa aacgtttcac cggcaaccag attgaaccct
    13441 ttgggattgc aaagggtcac aatagcgaca tcgccctctt tggtataatt aaaatgcttt
    13501 ccgtaactca tgcccatacc tccatcaaaa tattttgttc ccgtaattct ctatcagtaa
    13561 tatacttgtt acgaagcagg cttgtcaatt tgcatctcgc atacctgtta tttcagcagg
    13621 ggtgaaatcg ctttaaattc aggagttccg gcaactttaa gcaaatcaaa gacaatcgtt
    13681 tcactattgg tgatgactgc ccccagatca cgcattaaat ccagtccgct gcaataattg
    13741 attgcaaaac gcgagcagac agcatcctgg ggcagatgaa cggtatagcc tgcctccacc
    13801 atgtcccgaa ccgtctgaaa gacacaaaca tgcgtttccg agccggttac aatcaaggtt
    13861 ttgcgatcct ttggcaggat ttccagcaac tgctcactca cactgaaaga tactttttcg
    13921 atgtattgat agtcagccaa atggtcttta atctcagcgg cggttggtcc caacccgcgg
    13981 ggatattgtt ccgagacaac cattggaata ccgagctgtt tggccgtttc gatcaacagc
    14041 gccgtgttct tgtagacccg ttcacgttcg ggcatggccg acattaaacg ttcctgaata
    14101 tcaatgatca ccagtactgc atcttcctta tcaatatggt atttgttcat gtttgccctc
    14161 ctagtccgtt gtcctgtaat aattacctgc ttaaacaggt attaataata taacaaaagt
    14221 tgtctgctaa tgcatccggc agctgtgcta aacttgtttt agcgaatacc acagggagtt
    14281 gaatacggtg cggaatttta gaacaatcat tggttttggg ttaattctgt tcatcacgat
    14341 aaccatggtg atcatttccc agcctgccat caaagcgacc cggagcgggc atgaaccggt
    14401 caaaaccagt gaaaccataa gattattggc cacaggcgat atattgatgc acaataccgt
    14461 catcgctgcc ggattgaacc atggagaata tcaatatgag caaatgtttg cccccatcaa
    14521 gcatatcgtg gaatctgccg actatgccat gattaatctg gaagctgcgc tggccggtcc
    14581 gaaaaacgga tatacgggct atccgctttt caatgcgccc gacagcttgg caaccgccct
    14641 gaataatacc ggatttgatc tggtcaccaa tgccaacaat catatccttg atcgaggcta
    14701 tgaaggcgcc atacgtacac tggatgttct ccggcaaagc ggtctggaca ccctcggcgt
    14761 caatccatcc caacaggaac gagaaactcc cctgatcaaa gacatccgcg gagtgaaagt
    14821 aggtttttta gcctatggct acgataccaa cgggattccc ctgccccagg acaaaccgta
    14881 tttttacaat cttttggatg aacaagtcgt tcttcaggac attactgccc tgcgtccgca
    14941 ggttgacatc ctggtggtta gcttgcattg gggggttgaa tacagcccct acccgacaga
    15001 aaaacaaatt gccatggcgc agcgtttttt catccaagga gctgatgtaa ttttcggcgg
    15061 gcatccccat gtcttgcagc cggcagaagt gttgacggtc agcggacaaa agaaattcat
    15121 cgtatattca atgggcaact gtctggggga tcaaaatgga gtcgagcgaa acagcggcgt
    15181 gatcatcagc ctggcattta aaaaagactt gctgaccggg aaaactgaac tgcaatcatt
    15241 tgattatatc cccacgtttt cccattccta tattgaaaac aatcgagaaa actttcgcgt
    15301 cattcctgtg ccggaaacca tacaaaaagt gaaagctcat caagaccccc atctgcaaga
    15361 cgacagtctt gctttattgg aacatatttc acaagcctgc caggcaaaaa tgagctcccc
    15421 ttgatgctta tcgctcaaac attgactgcg tcccgagacc gtcatgatca ttaagaacct
    15481 taaaatcctt gaaacaaacc gatacgatgg atccagcggt gccgctttcg atggtcaatt
    15541 tggctttatg gcgggcagca atgtttttac agatggataa acccagccct gtcccctgtt
    15601 ctttggtggt aaaaaaaggt gtaccgatgc gctccattat tgctgcatca atccccgcgc
    15661 cttggtcttc aatcgccagg atgatatgat tgccttggga taaggttcga atggtcaggg
    15721 tgaatcctgg ttgcatcgct tccaatccgt tgtttaccaa attgacaatc aactggcgaa
    15781 tctgattgcc atccatatac acttcattaa tattccccaa atccagtata attctatggt
    15841 tgttgcgggc agcctccacc gcaataaatg gatatagggt tcgtatcacg gcattgagat
    15901 cctgccagga aaaacgcact tccttttcct tggccatgga caagtaatct gccatcaatt
    15961 gattggcacc atccaattcc tcaatcatca atgcaaagta attgccatat ggctgcaaat
    16021 cctcatgatc gcccagaaac tgcaaaaacc cacgtacaga cgtgatttga ttgcgaacct
    16081 cgtgcaccag tccggcagtc atttgtccaa tggttttcaa ctgttccagc cgggccagtt
    16141 cgttttccag gcgaatctct tccgtaatat cgcgagtgac aaatacggct ccggtgattt
    16201 gctgttcttt attgtaaact gccatcccct gggtttccaa atgaatgtat tgttcatcgc
    16261 ggcaacgaaa acgtatcctt gttgttttaa actgaccggt atccagccca agtttaaaca
    16321 aagacctgac cataaacagt tcttcgggat gaatgtaatc aaatatggat tggtgcagca
    16381 agctttccgg ttcataaccc aaaagcgttt ggtgggtgtt gcctaaatat tggtattccc
    16441 cttccaggga aacctgcgag atcaacaaca tgttgccgac aattctatgt atgatactat
    16501 ccagataggg taattggttt aacgttaaag gggccgcatt cattatttca ccgcctattg
    16561 aacagttatt gtctatatta aaattaaagt aattatacaa tattcgattc gatattacaa
    16621 ttttgtcgtc attgtaacac aatttcatct tgatttatta caatcgtatt ttaataattt
    16681 gcagatggca ccatggattt ttgcccgagt gaatgaattg atgaatacaa acaactacga
    16741 ttgagataaa ataaaggcaa gcaaaaagga aaggagcgga gaatatgagc accaacgaac
    16801 tgaacattct aaagaaagcg atcttaatcg aggccgaggg agaaaacttt tatcagttgg
    16861 cggctgacca aagcaataat caagaagtga aaaatgcttt tctatatttg gccgaagaag
    16921 aaaaacaaca tggtacctgg ctgcgccaat tgatgcagtc tttggccccg ggcaacagct
    16981 tcgatctgaa acaactggaa agtcaggcgg aaatgagcct gccacccatc tttgattttt
    17041 cccaattgaa aggccaacag aaaaacaccc tggaaatatc agtttttcat atcggaattc
    17101 tgatggaaaa ggcatccttg gactattatc gtgaagccgc tgaaaaatct actaatccag
    17161 aagcgcaaaa actatatgag accctttcca aatgggaaat gcatcatctt aatgaattgg
    17221 agaaagtata tgatggatta agcgaagact ggtttgacca tcaaggtttc tcgcccgcct
    17281 aagcattgag aggcgttgtt tattgattcc ctgcttctct gtttggtaca tgtcgatccc
    17341 ggcaagattt ttattgtatt ttccccggca taatatgcta taatttattc tgctgtgggg
    17401 gcgtggctca gctgggagag cacctcgttc gcaacgaggg ggtcaggggt tcgaatcccc
    17461 tcgtctccac cataattgtc ataaatatat attgataaaa aaccatctcg tgagaggtgg
    17521 tttttttg
//
LOCUS       DAOTHZ010000045        17514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_3903_length_17514_cov_6.117418, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000045 DAOTHZ010000000
VERSION     DAOTHZ010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 17514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17514
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_17514_cov_6.117418"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /gene="carB"
                     /locus_tag="P5549_06595"
     CDS             complement(<1..1701)
                     /gene="carB"
                     /locus_tag="P5549_06595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRW12777.1"
                     /translation="MPKRTDINKILIIGSGPIVIGQACEFDYSGTQACKALRKLGYQI
                     VLVNSNPATIMTDPNMADATYIEPLNLQRITDIIAKERPDAILPNLGGQSALNLCSEL
                     SQAGVFEKYAVKVIGVQIDAIERGEDRIAFKETMNRLGIEMPRSKPAYSIEEAETIAA
                     ELGYPVVIRPAYTMGGTGGGLVYNVEELRIVANRGIAASLVGQILIEESVLGWEELEV
                     EVVRDAKNQMITVCYIENIDPMGVHTGDSFCSAPMLTISQQLQERLQKYAYSVVEAIE
                     VVGGTNVQFAHDPKTDRVVIIEINPRTSRSSALASKATGFPIAMVSAMLAAGLTLDEI
                     TYWKEGTLDKYSPGGDYVVIKFARWAFEKFPGSFDQLGTQMRAVGEVMSIGKNYKEAF
                     QKAIRSLEINRYGLGFAKNFNQLSVEELLSRLAEPSSERQFIMYEALRKGATVEHITG
                     LTHIKPWFVEQMLELVRLEEAILACKGCQLPDELLIQAKKDGFADRYLAMLLDEHETS
                     IRQRRVTLGIKEAWEAVPVSGVDNAAYYYSTYNAPDTTTASDRPKVMILGGGPNRIGQ
                     G"
     gene            complement(1938..3221)
                     /locus_tag="P5549_06600"
     CDS             complement(1938..3221)
                     /locus_tag="P5549_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HRW12778.1"
                     /translation="MEKAQSLNKNRRRMRVRVVLRAAISGALLGGMIGAFAKYNPSID
                     PVAFALGFGGLGGLFIGLWTSTENAHEYVEPSEKLADFAQRVADGDLTGQIDQFHKGY
                     MGNLAESINNMTNRLKHLISETNLISQQVYTSSEKLLHLSRENSSAAQDVSLAISNIA
                     DGADQQAVSTQNTTNLILNMADTFAAIADNTQKCVQTSIKTRQAVQSGVEAVNLHNQR
                     MKESRQALEAVSQAVTMLDENSARIEEIVAVIRGIADQTNLLSLNAAIEAARAGEHGR
                     GFAVVAEEVRKLAEQSENSASEIAVLIKQMQTNTRQVVNDMETTKNVYIQQIKAIEST
                     NNVFETIVEEVGNIDNEVVEISASTQEVSASTEDLVTAVKAISMIAKQIAANSVEVSQ
                     QSQKQADALHTVINEFETLRDNLANIEKLVASFTI"
     gene            3520..6303
                     /gene="ileS"
                     /locus_tag="P5549_06605"
     CDS             3520..6303
                     /gene="ileS"
                     /locus_tag="P5549_06605"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRW12779.1"
                     /translation="MAKGKYDETLNLPSTEFPMRANLPNREPEILKFWEENDIYQQVL
                     EKNRGREKFILHDGPPYANGDIHLGHTLNKILKDIIVKQHAMLGYDSPYVPGWDTHGL
                     PIEQQAIKALGIDRHRTDVVEFRRHCHDYAMKYVDIQREEFKRLGVGGDWEHPYLTLA
                     PEFESEQIKVFGAMANKGYIYKGLKPVYWCGDCETALAEAEVEYGDKISPSIFVKFPI
                     KDGKGLVPDDAFVIIWTTTPWTLPANTGVCLHPDYEYVLVQVSGEKYVLAKGLLESVA
                     SQLSWNEVALLKEFRGQELERAVCRHPFMERDSLLILGRHVTLEAGTGCVHTAPGHGE
                     DDFYVGREYGLEVISPIDNSGCFTEQAGKFKGLYVHKANQNVIDELQAGGMLLKTEKY
                     EHQYPFCWRCKQPIIFRATDQWFASIDGFRQDTLKAIDEVEWIPGWGRERIYNMVRDR
                     GDWCISRQRTWGVPIPIFYCDSCGETIVSKETIEHISKLFGEFGSDIWFIKEAAELLP
                     EGFACPQCGQQQFSKETDIMDVWFDSGSSHMAVLETRPDLQWPADLYLEGSDQHRGWF
                     NSSLSTAVAVRGTAPYKKVLTHGFIVDEKGYKMSKSLGNVVDPLQMIKELGADILRLW
                     VSSADYRNDISVSSNIIKQSSEAYRKIRNTCRFILGNIADFDSRQDSVAYENLGELDQ
                     WALLKLEQVVQRVTKAYADYEFHVVFHTVHKFCTVDLSSIYFDIIKDRLYCNDKNDPA
                     RRASQTVLWQLINDLVVILAPVLAFTCEEIWSYIRKENDPVSVQLLSWPQINGAHQNK
                     ELENKIDRLLQVREVASKAMEEARGRKTIGHSLGAWLTVYADDEWLELLQNTNELDKI
                     LIVSRVDIEPAVNRGEDAVALEEINGLWVKVQAAAGQKCERCWIIDLSVGSDPQHPSL
                     CQRCAQVIAQMEG"
     gene            6305..6595
                     /locus_tag="P5549_06610"
     CDS             6305..6595
                     /locus_tag="P5549_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRW12780.1"
                     /translation="MASPEEGNRIIITVLGHDRVGIIAMVSNILADHDINILDISQTI
                     LQGFFTMVMVVDVSRSKIDVKELREILGGKGLEMGLQITAQHEDLFKYMHRI"
     gene            7028..8395
                     /locus_tag="P5549_06615"
     CDS             7028..8395
                     /locus_tag="P5549_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HRW12781.1"
                     /translation="MPFSISKDEILETVAMLHTENLDIRTITMGIDILSCHAASGKQC
                     GQKIYDKILCHASQLVKVGDDIEKEYGIPIINKRISVTPVALVADGLGQDDMIEIGRH
                     MDKASGEVGVNFIGGFSALVHKGFSRGDSALLAAIPETLSVTERVCSSVNLATTKAGI
                     NMDAVAGMGRIIKETARLTADRDGLGCAKLVVFCNAAEDNPFMAGAFHGVGEPECTLN
                     IGVSGPGVVLNAVRKYPDADLGELANIIKKTAFKVTRSGELVGRVASQRLGVSFGIVD
                     LSLAPTPAIGDSVADILESMGLEMVGAHGSTAALAMLNDAVKKGGAMASSYVGGLSGA
                     FIPVSEDQGMINAVEAGALNIEKLEAMTCVCSVGLDMIAIPGDTPAEIIAAIIADEAA
                     IGMINRKTTAVRLIPAPGKKVGDWVEFGGLLGRAPIMKVNQMSPARFIGRKGRIPAPI
                     HSLNN"
     gene            8450..8767
                     /locus_tag="P5549_06620"
     CDS             8450..8767
                     /locus_tag="P5549_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HRW12782.1"
                     /translation="MEQQPGLPDEKQTLETLGNKIDELSLNMEKLGIAEYVAMIENPR
                     RLFWVNFWQGVARGFGMAIGFTILAGLVIYLLQKIVVLNMPLIGSFIAEIVDIVQNQL
                     YMQ"
     gene            8806..9249
                     /locus_tag="P5549_06625"
     CDS             8806..9249
                     /locus_tag="P5549_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRW12783.1"
                     /translation="MDQRQQLIAKKQQLEQLIKNKLEVVSTPMAEWNDELSSYDQHPG
                     DQATDLFEREKELSMLELLQFELEKVDDALQRSVQGQDSICSHCGGPIEPRRLERLVN
                     TTLCARCAHQYDGAYRADYEIAPQPGVISDVEQSFPGTSYEVFQD"
     gene            9290..10483
                     /locus_tag="P5549_06630"
     CDS             9290..10483
                     /locus_tag="P5549_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRW12784.1"
                     /translation="MRIGIFTDSYKPYTSGVVTSITTFKEELTRLGHEIHIFAPSYPH
                     YVDDEPNVYRFYSVPSPTNTDFTLAIPIYPGLGTLVKHLNLDIIHVHSPMIMGRVGLR
                     YARKYRIPLVFTYHTRYDQYVHYVPVAHDLAKEMTAKYSSSFCNHCDHIITPSEDIHT
                     IIRESEVRKPVSVIPTGIPLQHFQKGDQGWLRANYAIPQENKILLYVGRLTKEKNLPF
                     LIKTFLLVKEQLPQTTLVLTAAGPLEDDLKALGRKFGLGSEELVFTGAVPYEKLVNVY
                     HSADLFVFSSLTETQGLVLTEAMACGLPVVAVRASGVQDMVDDGVDGLLTELNMEEMA
                     AAIVRVMEDDALYAQLQHHALQKAEAMSARSMALQLEDVYLRLNKGRTYRSRRLLNVS
                     NWFSN"
     gene            10518..11930
                     /locus_tag="P5549_06635"
     CDS             10518..11930
                     /locus_tag="P5549_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRW12785.1"
                     /translation="MSLRLIMAIFVGKLAIVFSRLAGNQGTDFPGRLARRIYPPILTV
                     LAGQVKKEIIMVTGTNGKTTTSNMIAGIFRENGSTCVHNRAGANMLTGITTAFIKATN
                     LSGRCSFDYALLETDEANVPLLLQEVQANLLLVTNFFRDQLDRYGELDHTIQLIKQAA
                     AGRSLELLLNADDPLQSDIAACTGCQSRYYGFEDTIYDELTSADSREGRYCVQCGQAL
                     LYERYHYAQLGQYHCPQCGNRNPCRDYTGQQLEMDPGIRLQVNDLPLQSSYQGFYNAY
                     NILAAVSMGLRLGIDAEVIQRAVVNFQPRAGRLERFTIAGKPCLLVLVKNPTGLNQSL
                     SMLLLDPTQKSLFFALNDNAADGRDISWIWDADVEIMTRSEARIQQIICSGQRSGDIA
                     LRCKYAGFDPDKLSIESRLQEGIALAVAQAEEAAYILCTYTALFECRQIMLKMQKQSD
                     GTSPLVQPSPARQAQEGGVQ"
     gene            11930..12667
                     /locus_tag="P5549_06640"
     CDS             11930..12667
                     /locus_tag="P5549_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003023017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRW12786.1"
                     /translation="MQETFVIAHMYPDLMNLYGDRGNLLCLQQRIRGYGHRCEIRSLG
                     LEDQLDFSELDMIFMGGGSDREQGLVYHDLLHKAETLRQEIDNGLPALCICGAYQLLG
                     RYYQAPDGTMLQGLGFFDLYTEGRAPRLIGNILLESAVAGEKISVVGFENHGGRTYLH
                     DPDLKPFGTVIKGHGNNGEDGGEGLWYKNLIGTYLHGPLLPKNPAIADYFIQTMAARR
                     GLTLTGTLDDTIEHFAHQQVKNKILPK"
     gene            12840..13871
                     /locus_tag="P5549_06645"
     CDS             12840..13871
                     /locus_tag="P5549_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRW12787.1"
                     /translation="MFAIRLEQKALTLYPFPADLINRRIREVLPEPNEEIGSLVDYLL
                     AGSGKMLRPRLVFLSASLYPHEPQLVIDMAAAVELVHLASLVHDDVIDQSDCRRGRDS
                     LNHCFGNLASVLTGDYLFATAFQLVNRHGQQAILNTLTSTIQTMCAGEIQQMQLAFDV
                     EISEADYLQKSYRKTACLFAACCRIGALASVMPDAEAAALEEYGLCLGYAYQIIDDLL
                     DFMSDRDKLGKPCGSDLREGNITLPVICLLREDEPAAWLRAAIDQKDFSDVMIENVTS
                     ACINHGAIDKCLQLAFDFIARGLAQLESLPPGPARKELMSMGKLLLKNYYRSLRCDLH
                     MPEEERTSS"
     gene            13868..14959
                     /locus_tag="P5549_06650"
     CDS             13868..14959
                     /locus_tag="P5549_06650"
                     /inference="COORDINATES: protein motif:HMM:NF022233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraprenyl-beta-curcumene synthase family
                     protein"
                     /protein_id="HRW12788.1"
                     /translation="MNMTVIPRSKPETSRFHSGQSRILAHYICLTLPRVKKELQRWRT
                     WAAACPDPVLAAQAMSSLHAKDFHCYGGAVFAVGAEEQEENLITWITAYQTICDYLDN
                     LCDRADCLDGLAFRRLHSALLDALQPQQEMSDYYAFYPHQKDGGYLHHLVEVCRSCLA
                     KLTGYDCVKAEIMALVEHYIDLQVKKHLDWILREHELIRWAEAQAGEYEDLYWQEFAA
                     ASGSTLAVFALLAQAASEQVSEQQVQALRHAYFPWICGLHILLDYLIDREEDRLGGDL
                     NFTFYYQDEAVMIERLQHFIRQSQTCLNDLDNPVFTRTVIEGLLAMYLSDRKVKQLHL
                     QPTATILLNASGPSTWHVYRLCALVRRFF"
     gene            complement(15066..16637)
                     /locus_tag="P5549_06655"
     CDS             complement(15066..16637)
                     /locus_tag="P5549_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12789.1"
                     /translation="MKNQFHTSRPSSGCFASKNNGANNSGNAARGMQNPSALTANYAS
                     WGCRPARTDSNSVSSCLRAVNLVQNPSFEEEYSYWWTNNVYIGNTNVFEGTAVAVMGS
                     GVASLHQELDLLYGAVCRRLFFSFNAMGGAESCYNGTLTAQIVWLDKEYNQIGIGLRM
                     FVPDGRITNTARLTFFALTDQVPAQAVYARISFSKGQGTSSDAILIDQVLLAPLAGYD
                     LLTNSDFESDLLGWTANPTTAFISDHKLSLEGAGHVRTHFNGTLTQDVYIRNLPPGSS
                     FLFSFAAGGVGAVMLYVHVDWLDSNKNPIGSGLSLQIPNETLSLQGNYLSYLNLTEPT
                     LPGTAYARIIFDAIVPNSSCYLLLDQVLFARVMSDNLIVNPSFENGLNDWEATYVTLV
                     QRNDVYEGAADAGIGQIGGALWQEIDLTQAEGHSYLFSTGLGFRRTSSEATFGTMLMK
                     VVWLDPNRNEIGLGLCLIGTSSNQTGSEHQWVPYVGITEPAPPGTAGARIQFTKTEST
                     DGYIEIDNVVFGRLI"
     gene            17066..>17514
                     /locus_tag="P5549_06660"
     CDS             17066..>17514
                     /locus_tag="P5549_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate CoA-transferase"
                     /protein_id="HRW12790.1"
                     /translation="MKWQEYYDSHKTTAEEAVKVIQSGDRVVLGHACGEPQLIVDALV
                     ARADELEGVEIAHEVPMGKGTYCLPQYARSFRHNALFSGKPSRQAIREGRADYTSIYL
                     HELPILFTSGQFPIDVAMVTVSPPDELGRVSLGISVDYTLQAIKSAKA"
BASE COUNT         4202 a         4519 c         4674 g         4119 t
ORIGIN      
        1 gccctgtccg atgcgattgg ggccgccgcc caggatcatc actttaggac ggtcgctggc
       61 ggtggtggta tcgggcgcgt tgtaggtgga gtaataataa gcagcattgt cgacgccgct
      121 gaccggtaca gcctcccagg cttccttaat gcccagtgtt actctgcgct ggcggataga
      181 ggtttcgtgt tcgtccagta gcatggccag ataacggtca gcaaaaccgt cttttttagc
      241 ctggataagc agctcatccg gtaattgaca tcctttgcaa gcaaggatgg cttcctccag
      301 tcgcaccaat tccagcatct gttcaacaaa ccagggtttg atatgggtca gaccggtaat
      361 atgttcaacc gtggcgcctt tgcgcagggc ttcatacatt atgaactggc gttcgctact
      421 cggctcggcc agccgggaga gcagttcctc caccgacaat tggttgaagt ttttcgcaaa
      481 acccaggccg taacggttga tttccaacga gcggatggcc ttttggaagg cttccttgta
      541 atttttgccg atactcatga cttcgcctac cgcccgcatt tgggtgccca gctgatcaaa
      601 ggagccgggg aatttttcga aggcccagcg ggcaaatttg atgaccacat agtctccgcc
      661 aggagagtat ttgtcaagtg taccttcctt ccaataggtg atttcatcca gggtcaggcc
      721 ggcggccagc atggccgaga ccatggcaat cggaaatccg gtagctttgg aagccagtgc
      781 cgaggaacgt gaggtacgcg gattgatttc aatgatgacc acccggtcgg ttttgggatc
      841 atgggcgaat tgcacattgg tgccgcccac cacttcgatg gcttccacca cggagtaggc
      901 atacttctgc agacgttcct gcagttgctg gctaatggtc agcatagggg ctgaacagaa
      961 ggagtcgccg gtgtgaacgc ccatggggtc aatattttct atataacaaa cggtgatcat
     1021 ctggtttttg gcatcgcgca ccacctctac ctccagttcc tcccaaccga gcacggattc
     1081 ttcgatcagg atctgcccca ccagactggc agcgatgccg cggttggcca cgatgcgaag
     1141 ttcttccaca ttatagacca atccgccgcc ggtgccgccc atggtatagg ccggtcgaat
     1201 gactacggga tagcccagtt cggcagcgat tgtttctgct tcttcaatcg aataggctgg
     1261 tttgctgcga ggcatttcaa tgcccaatcg attcatggtt tccttaaaag cgatgcggtc
     1321 ttctccgcgt tcgatggcat caatctggac gccgattact ttgaccgcat atttttcaaa
     1381 aacccctgcc tggcttaatt cagaacaaag attgagcgcc gactgaccgc ccagattggg
     1441 caggatggca tcgggacgct ccttggcgat gatatcggtg atgcgctgca gattgagggg
     1501 ttcaatgtaa gtggcatcgg ccatgttggg atcagtcatg atggtggcgg gatttgaatt
     1561 gactaaaaca atctggtagc ccagtttgcg cagggctttg caggcttggg ttccggaata
     1621 atcaaattcg caagcctgac cgatcacaat cggaccggaa ccaatgatga ggattttgtt
     1681 gatgtcagta cgtttcggca tattttacct cccaatttat gtgtctcatg gaatgttttt
     1741 tagtaaggct catatgggta atctaactat taataataac aaattgatga gcggggttgt
     1801 atgtaatctt gtatgaatac agtatttatt tttcccaaca ggcaagagga aattataaaa
     1861 aagcctgggg aaggggaaga cggacatccc ttcatcaggc ttcagacaaa tgactggtga
     1921 tcggtgcggc aaactggtta tatggtgaag ctggctacca atttttcgat attggccagg
     1981 ttgtcgcgca gggtttcaaa ttcattaatg acagtatgca gggcatctgc ctgcttttgt
     2041 gactgctggc tgacttcgac ggagttggca gcgatttgtt tggcaatcat cgaaatggct
     2101 tttacagctg ttaccagatc ttcggttgag gccgaaactt cttgggtaga agcggaaatc
     2161 tccaccactt cgttatcgat attgccgact tcctcaacaa tcgtctcgaa gacgttgttg
     2221 gtcgattcaa tggccttgat ttgctgtatg tagacgtttt tggtggtttc catgtcgttc
     2281 accacctggc gggtattggt ctgcatctgc ttgatcaaga ccgcgatttc actggcggag
     2341 ttctcagatt gttcagccag ttttctgact tcctcggcta ccacggcaaa gccacggcca
     2401 tgttccccag cccgggcggc ttcaatggca gcattcaggg aaagcaggtt ggtctggtca
     2461 gcaatccccc gaattacagc gacgatctct tcgatgcggg cggaattttc atccagcatg
     2521 gtgacggcct gactgaccgc ctccagcgcc tgacggcttt ctttcatgcg ctggttgtgc
     2581 agattgaccg cttctactcc gctttgcact gcctggcggg tcttgatgga agtctgcacg
     2641 catttttgcg tattatcagc aatggcagca aaagtgtccg ccatgtttaa aatgaggttg
     2701 gttgtattct gggtggaaac cgcctgctga tcggcaccat cagcgatatt gctgatggcc
     2761 agggaaacat cctgggcagc ggagctgttt tccctggaca aatgcaatag tttttccgat
     2821 gatgtataaa cctgttgact gatgagattg gtttcactga tcaaatgttt gaggcgattg
     2881 gtcatgttgt tgattgattc agccagattg cccatataac ctttatggaa ttgatcaatc
     2941 tgaccagtta gatcgccgtc agccacgcgc tgggcaaaat cagccagctt ttcggatggt
     3001 tcgacatact catgagcgtt ttctgttgaa gtccacaagc cgataaataa tcctcccagg
     3061 ccgccaaatc ccaaagcaaa ggcaaccgga tcaatggacg gattgtactt ggcaaatgcc
     3121 ccaatcatgc cccccaggag tgcgccgctg atggctgccc gcaagacgac ccgtaccctc
     3181 atacggcgtc tgtttttatt aagagattga gctttctcca tacctttgtc actccaatac
     3241 atgcgaaatt agttgtgaag tagtttctgg ataatgcttt tattatcctc tattataacg
     3301 ttacttggca gaaaatatcg aaaaaatact tgacgccaaa cgctgccttg actgcaacag
     3361 ttgttatgtg gcggtctata tgctaaaatg tattacgttt taaaggcagg tatgctgaaa
     3421 agagcggcag cgaataaccg agtgcctttc gtctgtggga tgagaggttt tttgcgttaa
     3481 tgcgggccga tcggaaaata gaatgaggtg gttacgacaa tggccaaagg aaaatatgat
     3541 gaaaccctca atttgcccag tacggagttt cccatgcggg ccaatctgcc caatcgggaa
     3601 ccagagattc tgaagttctg ggaagaaaac gacatttatc agcaggtcct ggagaaaaac
     3661 cgcggccggg aaaaattcat cctgcatgac ggtcctccct atgccaacgg ggatatccat
     3721 ctgggccata cgctgaataa aatattaaag gacatcatcg tcaaacagca tgccatgctg
     3781 ggctatgatt ctccttatgt acccggctgg gacactcacg gattaccgat cgaacagcag
     3841 gccatcaaag ccctgggcat tgaccgccat cgtaccgatg tggttgaatt ccgccgtcat
     3901 tgtcatgact atgccatgaa atatgtcgat atccaacggg aagagtttaa acgcctgggc
     3961 gtcggcggtg actgggagca tccctatctg accctggccc cggaattcga aagcgagcag
     4021 atcaaggttt tcggagcaat ggctaataaa ggctacatat ataaaggatt gaaaccggtg
     4081 tactggtgcg gtgactgtga aactgccctg gctgaggcgg aagtcgaata cggcgacaag
     4141 atttccccct caatttttgt caaattcccg atcaaagacg gcaaaggact cgtgcccgat
     4201 gatgccttcg tcattatttg gaccaccacc ccctggacat tgccggccaa taccggtgta
     4261 tgcctgcatc ctgattatga atatgtgctg gtccaggtta gcggcgaaaa atatgttttg
     4321 gccaagggct tgctggagag cgttgcaagc cagctgagct ggaacgaggt tgcacttctc
     4381 aaggaatttc gtggccaaga gctggaacgg gccgtctgtc gtcatccctt catggagcgc
     4441 gactctctgc tcattctcgg ccgtcacgtt acgctcgagg ccggtaccgg ctgtgtgcat
     4501 accgctcccg ggcatggcga agacgacttt tatgtaggcc gtgaatatgg gttggaagtg
     4561 atttcgccga tcgacaacag tggctgcttt accgagcaag ccggcaaatt caaaggattg
     4621 tatgtccaca aggccaatca aaacgtcatc gatgaactgc aggccggggg catgctgctc
     4681 aagactgaaa aatacgaaca tcagtatccg ttctgctggc gctgcaagca gcccattatc
     4741 ttccgggcta ccgaccaatg gttcgcttcc attgatggct tccgccagga caccttgaaa
     4801 gccattgatg aagtcgaatg gattccgggc tggggacggg agcgcatcta caacatggta
     4861 cgcgaccgcg gcgactggtg catttcccgg cagcgcacct ggggcgtgcc cattcccatc
     4921 ttctattgcg acagctgcgg cgagaccatc gtcagcaaag aaaccatcga acacatcagc
     4981 aaattgtttg gggaattcgg ctcggatatc tggtttataa aagaagctgc tgaattactg
     5041 ccggaagggt ttgcctgtcc acagtgcggc cagcagcaat tcagcaagga aaccgacatc
     5101 atggacgtct ggtttgattc cggctcttcc catatggcag tgttggaaac cagacccgat
     5161 ttgcaatggc cggccgacct ctatctggag ggcagcgacc agcatcgcgg ctggtttaac
     5221 tcgtccctgt ccaccgcagt cgcagtgcgc ggcacggcgc cctataaaaa agtcctgacc
     5281 cacggattta tcgtggatga aaaagggtat aaaatgtcca agtcgctggg caacgtagtt
     5341 gatccactgc agatgatcaa agaactgggc gccgatatcc tgcggctgtg ggtttcttct
     5401 gccgattatc gcaacgacat ttccgtatcc agcaacatca ttaaacaatc cagtgaagcg
     5461 tatcgtaaga tcagaaacac ctgccgcttc attctgggca atatcgccga tttcgattcc
     5521 cgccaggaca gcgttgccta tgaaaacctg ggcgaactcg accaatgggc gctgctcaaa
     5581 cttgaacagg tggtgcagcg ggtcaccaag gcctatgccg attatgaatt ccacgtcgtg
     5641 ttccataccg tgcataaatt ctgcaccgtt gacttgagca gcatttactt cgatatcatc
     5701 aaggaccggc tctattgcaa cgataaaaac gatccggccc gccgcgcttc ccagaccgtg
     5761 ctgtggcagc tgatcaacga cctggtggtt atcctggctc cggttctcgc ttttacctgc
     5821 gaagaaatct ggtcctatat ccgcaaggaa aatgatcctg tcagcgtaca actgttaagc
     5881 tggccccaga tcaacggggc tcatcaaaat aaagaacttg aaaacaaaat cgaccgtctt
     5941 ctgcaggtgc gcgaagtcgc cagcaaagcc atggaagaag cccgcggccg caaaaccatc
     6001 ggccattccc tgggcgcctg gctgacggtc tatgcggatg atgagtggtt ggaactgttg
     6061 cagaacacca acgaacttga taaaatcctg atcgtttccc gggtcgacat cgagcctgcc
     6121 gtcaatcgcg gtgaagatgc cgtagccctg gaagaaatca acggcctgtg ggtcaaagtg
     6181 caagccgccg ccggccaaaa atgtgaacgc tgctggatca tcgatctgtc cgtgggaagc
     6241 gatccccagc acccaagttt atgtcagcgc tgtgcgcagg tgattgcgca gatggagggt
     6301 taacatggcc agtcccgaag aaggcaatcg catcatcatc accgtgttgg gccacgaccg
     6361 ggtcggaatc attgccatgg tttccaatat cctggccgac catgatatca acatcctgga
     6421 catcagccag accattctgc agggattttt caccatggtc atggtcgtcg atgtcagccg
     6481 cagtaaaatc gatgttaagg aactgcgtga aattttgggc ggcaaaggcc tggagatggg
     6541 cctacagatc accgcccagc atgaagacct gttcaagtat atgcaccgga tttaacttta
     6601 tcttggggtt ataaatgtat tgttctctga ggttaatact taagtccgct tggggccggt
     6661 cttttgactg actagaattg cagtattagt gctcatttgc tggctgggaa tctttagact
     6721 aatacgtgag tctcgttttt gggtaacgta taactttatt aaactgttag cgttctttgc
     6781 gccgttgcgt tccagcgcac ggctgtcgcc ttacccccgg cgctccactt aagcattaac
     6841 ctctgcgata ggtttgctta tatgagtaca ttaattaatt tagagccggg gcaatggcac
     6901 agccgagccc aagatggcga agcccgggct aatggagcaa gtcaagaacc gtccccaact
     6961 tgcgtaacga ttacagagag caagtcaaga accgtcccca acttgcgtgg aatgaaggag
     7021 atggagtatg cctttcagca tcagcaaaga tgaaatcctg gaaaccgtcg ccatgttgca
     7081 tacggagaac ctggatatcc gtaccatcac catgggcatt gacatattaa gctgccatgc
     7141 cgccagcggc aagcagtgcg gacagaagat ctatgacaaa attctctgcc acgcctccca
     7201 attggtaaaa gtgggcgatg atattgaaaa agaatacggc ataccgatta tcaataaaag
     7261 aatttccgtt acccccgtgg ccctggtcgc cgacggactg ggccaggatg atatgatcga
     7321 aatcggacgc catatggaca aagcctccgg cgaagtcggc gtcaacttta tcggcggctt
     7381 ctccgccctg gtgcacaaag gatttagccg cggggacagc gccttgctgg ctgccatacc
     7441 cgagaccctt tcagtcaccg aacgggtctg ttcctccgtc aatctggcca ccaccaaggc
     7501 cggcatcaac atggatgccg ttgccgggat gggcaggatc atcaaggaaa ccgcccgcct
     7561 gaccgccgat cgtgatgggc tgggatgtgc caaactggtg gtcttctgta atgctgctga
     7621 agacaatcct ttcatggccg gcgcctttca cggggtgggc gagccggaat gcaccctcaa
     7681 cattggcgtc agcggaccag gggtcgtgct caatgcggtc cgcaagtatc ccgacgccga
     7741 tctgggcgag ctggccaata tcatcaagaa gaccgccttt aaagtaaccc gcagcgggga
     7801 actggtcggc cgggttgctt cccagcgact gggggtttcc tttggcattg ttgatctgtc
     7861 gctggcaccg acaccggcca tcggcgacag tgtagccgat attctcgaat cgatggggct
     7921 ggaaatggtc ggcgcccacg gttccaccgc cgccctggcc atgctcaatg acgctgttaa
     7981 aaaaggcgga gccatggcct cctcctatgt gggcggactg tccggcgcct ttattcctgt
     8041 cagcgaagac cagggcatga tcaacgcagt ggaagccggt gcgctgaata ttgaaaaact
     8101 ggaagccatg acctgcgttt gctcggtagg tctggatatg atcgccattc ccggtgatac
     8161 accagctgaa attattgccg ccatcattgc cgacgaagct gccatcggca tgatcaatcg
     8221 caagaccact gctgtcagac tcattccggc acccggcaaa aaagtagggg actgggtgga
     8281 gtttggagga ttgctgggcc gggctccaat catgaaagtc aaccagatgt caccggcccg
     8341 cttcattggg cgcaaaggcc ggatccctgc cccgatccat tctttgaaca attaagtatg
     8401 attcgcctga cagctgggga ataatagttt tgagaagcgg gtgattttat tggaacaaca
     8461 acccgggttg cctgacgaaa agcaaaccct ggagacactg ggcaataaaa tcgacgaact
     8521 gtccctcaac atggaaaagt tgggtattgc cgaatacgtc gccatgattg aaaacccccg
     8581 ccgcttgttc tgggtcaact tctggcaggg agtagcgagg ggatttggga tggccattgg
     8641 gtttaccatc ctggctggtc tggtgattta cctgctgcag aagatcgtgg tattgaatat
     8701 gccgctgatc ggcagtttta tcgccgaaat tgtggatatc gtgcagaacc agctttatat
     8761 gcagtaaccg cttttcagac ttcactggaa agacaggtga cggctgtgga tcaaaggcag
     8821 caattaatcg ccaaaaagca acaattggaa cagctcatca aaaacaagct ggaagtcgtc
     8881 tccaccccca tggcggagtg gaacgatgaa ttgtcttcat atgatcaaca tcccggcgat
     8941 caggccactg atctgtttga acgggaaaag gaactgagca tgctggaact gctgcagttc
     9001 gaactggaaa aagtcgatga cgcactgcag cgcagtgtac agggacagga cagtatttgc
     9061 agccattgcg gcggaccgat agaaccgcgc cgtctggaac ggctggtcaa cactactttg
     9121 tgtgcccgtt gcgcgcatca gtatgacggc gcttatcgcg ctgattatga aattgccccg
     9181 cagccggggg ttatatccga tgtggaacaa tcttttccgg gtacgtccta cgaagtgttt
     9241 caggattaaa gcaacccctg acccggacca ccaccgagga ggataagagt tgaggattgg
     9301 catttttacc gacagttaca aaccctacac cagcggtgtg gtgacttcca ttaccacttt
     9361 caaagaagaa ctgacccgtc tgggtcatga aatacacatt tttgcgccca gttatcccca
     9421 ctatgttgat gacgaaccca atgtgtaccg gttttattcg gtaccgtcgc ccaccaatac
     9481 cgattttacg ctggccattc cgatttatcc gggcttggga acgctggtca aacatctgaa
     9541 cctggacatc atccatgtgc actcccccat gatcatgggc cgggtggggc tgcgttatgc
     9601 gcgcaagtac aggataccgc tggtcttcac ctatcacacc cgctatgacc agtatgtcca
     9661 ttatgtgccg gttgctcatg atctggccaa agagatgact gccaaatata gttcttcctt
     9721 ctgcaatcat tgtgatcata tcatcacgcc gtcggaagac attcatacca tcatccggga
     9781 gagtgaggtg cgcaagccgg tctccgtcat tcccaccggc attcccctgc agcactttca
     9841 aaaaggcgac caaggctggc tgcgggccaa ttatgcaatt ccgcaggaaa ataaaatcct
     9901 gctctatgta gggcgtttga ccaaggagaa aaacttaccg tttctgatta aaaccttcct
     9961 tctcgtcaag gagcaactgc cgcaaacgac cctggtgctg accgctgccg gaccgctcga
    10021 ggatgattta aaggcgctgg gacgaaagtt cggtctgggt tcggaagaac ttgtttttac
    10081 cggggccgta ccctatgaaa agctggttaa tgtttatcat tcggctgatt tgttcgtgtt
    10141 ttcctccctg acggaaaccc aggggctggt gctgaccgaa gccatggcct gcggcctgcc
    10201 ggtagtggca gtgcgggcca gcggggtgca ggacatggtc gatgacgggg tggacggact
    10261 gttgaccgaa ttgaacatgg aggaaatggc ggcggccatc gttcgagtga tggaggacga
    10321 cgctttgtat gcgcagctgc agcaccatgc cctgcagaag gccgaagcaa tgtcagcccg
    10381 cagcatggcg ctgcaactgg aggatgttta cctgcggctg aacaaaggcc gcacctatcg
    10441 ttcccgccgt ttattgaatg taagcaactg gttcagcaat taaaccagcc gctgcaagca
    10501 ggggagagaa gaccattatg agcctacgcc tgattatggc catttttgtc ggcaagctgg
    10561 ccatcgtgtt cagccgcctg gccggcaatc aaggcaccga cttccccgga cggctggccc
    10621 gccggattta tccgcccatt ctgacggtcc tggccgggca ggttaaaaaa gaaatcatca
    10681 tggtgaccgg gaccaacggc aaaaccacca ccagcaatat gattgccggg attttccggg
    10741 aaaacggcag cacctgtgta cataaccgtg ccggcgccaa tatgctgacc ggcatcacta
    10801 ccgcctttat caaagccacc aatctgagcg ggcggtgcag ctttgattac gccttgctgg
    10861 aaaccgacga agccaatgta ccgctgctgc tgcaggaagt acaggccaat ctgctcctgg
    10921 tgaccaattt ctttcgcgat caattggacc ggtatggcga gcttgatcac accatccaac
    10981 ttatcaagca agccgctgca ggacgcagcc tggaactgct gctcaatgcc gatgatcccc
    11041 tgcagagtga tattgccgct tgcaccggat gccagagtcg ctattacggc ttcgaggata
    11101 caatctatga cgaactgacc agtgccgaca gccgtgaagg ccgttactgt gtgcagtgcg
    11161 gtcaagcact gctttatgag cgctatcatt atgcccaact ggggcaatac cactgtcccc
    11221 agtgcggcaa ccgcaatccc tgccgtgact acaccggtca gcaactggaa atggacccgg
    11281 gcattcgcct gcaggtcaac gacttgcctt tgcagagttc ctatcagggt ttttacaatg
    11341 cctacaacat tctggcagcg gtttccatgg gtctgcgcct gggcatcgac gccgaggtca
    11401 tccagcgcgc cgttgtcaac tttcagcccc gcgccgggcg actggaacga ttcaccattg
    11461 ccggcaagcc gtgtctgctg gtgctggtca aaaatcccac cggactcaac cagagcctca
    11521 gcatgctgtt gctcgacccg acgcagaaaa gcctcttttt tgccttgaac gacaatgccg
    11581 ccgatggacg tgacatctcc tggatctggg atgctgacgt ggagatcatg acccggtccg
    11641 aggccaggat tcaacaaatc atctgttccg gccaacgcag cggcgatatc gccctgcgct
    11701 gcaagtatgc aggttttgac cccgacaagc tttccatcga atcacgattg caggagggca
    11761 ttgccctggc ggtcgcccag gcggaggagg cggcctacat cctttgcacc tataccgcct
    11821 tgtttgaatg ccggcagatc atgctgaaga tgcagaaaca atccgacggg acatccccgt
    11881 tggtgcagcc gtctccagcc cggcaagcgc aggaaggagg cgtgcagtga tgcaggagac
    11941 attcgtcatt gcccatatgt atccagattt aatgaacctt tacggagacc gcggcaatct
    12001 gctctgtttg cagcagcgca tccgcggcta tggccataga tgcgagatcc gctcgctggg
    12061 gctggaggac caactggatt tttctgaact cgatatgatc tttatgggcg gcgggtccga
    12121 ccgcgagcaa ggtctggttt accacgatct gctccataag gcggaaacgc ttcggcagga
    12181 aattgacaac ggactgcccg cgttgtgcat ctgcggagcc tatcagttgc tgggccgcta
    12241 ttatcaggcc cccgacggta cgatgctgca gggtctgggc ttttttgacc tctataccga
    12301 agggcgcgcc ccgcgtctga tcggcaacat cctcctggaa agcgcagtgg caggagagaa
    12361 aatcagtgtg gtcgggtttg aaaaccatgg cggccgcacc tatttgcatg acccggacct
    12421 gaagcccttt ggcacggtca tcaaaggaca cggcaacaac ggcgaagatg gcggggaagg
    12481 actgtggtac aaaaatctca tcggcaccta tctgcatggc cccttgctgc ccaaaaatcc
    12541 agccatcgcc gactatttta tccaaaccat ggccgcccgc cgcggcctca ccctgaccgg
    12601 taccctcgac gacaccatcg agcatttcgc ccaccagcaa gtcaaaaaca aaatcctccc
    12661 aaagtaatat ctgcaacccg cgcaaatcgt ggggacggtt tggcgtgctc agccttcttt
    12721 tcagaatatt ccataagtta ataggcctga cgtattaact tatggaatat tctgaaaaaa
    12781 gaggcggtat agtttgctgc cgggatggca aaaataacgg caaacattat ggaaggggga
    12841 tgtttgccat tcgactggag caaaaagcac tgactttgta tccttttccc gccgatctca
    12901 tcaatcgccg cattcgcgaa gtgctgcccg aaccaaacga ggaaatcggc agccttgtcg
    12961 actatctctt ggcgggcagc ggcaagatgc tgcggccgcg cttggtattc ctgtccgcct
    13021 cgctttatcc gcatgaaccg caattggtga ttgacatggc cgccgcggtg gaactagttc
    13081 atctggcttc tctggttcat gacgatgtaa ttgatcagtc cgattgccgc cgaggccggg
    13141 acagcctgaa ccattgtttc ggcaatctgg ccagtgtgct gaccggggat tatttgtttg
    13201 ccacagcgtt ccaactggtc aaccggcacg ggcagcaggc gatcctcaac accttgacca
    13261 gtacgattca gaccatgtgc gccggtgaaa ttcagcaaat gcagctggct tttgatgtag
    13321 agatcagtga agcggattac ctgcaaaaaa gctaccgcaa aaccgcctgt ttgtttgcag
    13381 cctgctgccg gattggtgca ctcgccagtg tcatgccgga tgccgaagcg gcagccctgg
    13441 aggaatatgg actgtgtttg ggctatgcct atcagatcat tgatgatttg ctggatttca
    13501 tgtccgaccg cgacaaattg ggcaagccct gcggcagcga cttgcgcgaa ggcaacatca
    13561 ccctgccggt catctgcctg ctacgggaag acgagccagc ggcgtggctg cgcgcggcaa
    13621 ttgatcaaaa agacttttcg gatgtcatga tcgaaaacgt cacttcagcc tgtatcaatc
    13681 atggtgccat cgacaaatgt ttacagctgg cttttgattt tattgcccgc ggactggctc
    13741 aactggaaag cttgcccccc ggtccggccc gcaaagagct catgtccatg ggcaaactgt
    13801 tgctgaaaaa ttactaccgc agtctgcgct gtgatttgca catgccggaa gaggagcgga
    13861 ccagttcatg aacatgaccg tcattccacg ctcaaagccg gaaacgagcc gttttcactc
    13921 cggtcagtca aggattttgg cccattatat ctgcctcacc ttgccccggg tcaaaaaaga
    13981 attgcagcgc tggcgcacat gggctgccgc ctgtcccgat ccggttctcg ccgcccaggc
    14041 tatgagcagt ctgcatgcca aagactttca ttgctatgga ggagcagtct ttgccgttgg
    14101 tgcggaggaa caagaagaga atctgattac atggatcacc gcctatcaaa ccatctgcga
    14161 ttatttggac aatctttgtg atcgcgccga ttgtctggac ggactggcct ttcgccgctt
    14221 gcacagcgct ttgctggatg cgctgcagcc gcagcaggaa atgagcgact attatgcatt
    14281 ttatccgcat caaaaagacg gcggttattt gcatcatctg gttgaagtat gccgcagctg
    14341 tctggccaag ctgaccggtt atgattgcgt aaaagccgaa ataatggcgc tggtagaaca
    14401 ttatattgat ctgcaggtga aaaaacacct cgactggata ctgcgcgagc atgaactgat
    14461 ccgctgggct gaggcgcagg ccggcgaata tgaggattta tactggcagg aattcgctgc
    14521 cgccagcgga tccaccctgg cggtttttgc cttgctggcg caggctgcca gcgagcaggt
    14581 cagcgagcag caggtgcaag cattgcggca tgcttatttc ccttggatct gtggtttgca
    14641 cattttgctc gattatctca ttgatcggga agaggaccgg ctgggaggcg acctgaactt
    14701 taccttttat tatcaagatg aagccgttat gatcgagcgc ctccagcatt ttatccgcca
    14761 atcccaaact tgcctgaatg acctcgacaa tcctgttttt acccgcacag taatcgaagg
    14821 actgctggcc atgtatctgt ccgatcgcaa ggtaaagcaa ctgcatctgc aacccaccgc
    14881 gaccatcttg ctcaatgcct ccggtcccag tacctggcac gtttatcgcc tttgcgccct
    14941 ggtgcgccga ttcttctgaa aaatgaggcg cgcaaaaaaa tcagaaaatt ccataagtta
    15001 ataagcctga cgtatcaact tatggaatat tcagacaata aggcgtcctg tctctggggt
    15061 tgcttctaaa tcaggcggcc gaagacgacg ttgtcgattt cgatatagcc gtcggtgctt
    15121 tcggtcttgg taaactggat acgggctcca gctgtcccgg ggggagcggg ttcggtgatg
    15181 ccgacatagg gcacccattg atgttcactc ccggtctggt tgctgctggt gccgatcagg
    15241 caaagaccca ggccgatttc gttgcggttg gggtccagcc agacaacctt catcagcatt
    15301 gtgccaaagg tggcttctga actggtccgc cgaaaaccga gtccagtgct gaacagataa
    15361 ctatgccctt cagcctgggt caaatcaatt tcctgccaca gcgccccgcc aatttgcccg
    15421 atgcctgcat cggctgcgcc ttcataaaca tcattgcgtt gcaccagggt aacataggtc
    15481 gcttcccaat cgttcagacc attttcaaag ctgggattta caatcaggtt atcgctcatc
    15541 acccgggcga aaagaacctg gtccagcagc aaatagcagc tggaattagg gacaatagcg
    15601 tcaaatataa tccgtgcgta agccgtgccc ggcagcgtcg gttcagttag attaagatag
    15661 cttagataat tgccctgtaa cgacagcgtt tcgtttggta tctgcagaga cagtcccgag
    15721 ccgatcggat ttttgttgct atccagccaa tcaacatgaa catacagcat aaccgctcct
    15781 acaccgccgg cagcaaaact gaaaagaaag gacgaacccg gcggcaaatt gcggatataa
    15841 acatcctggg tcagcgtgcc gttaaaatgg gtgcgcacat ggccggcgcc ttcgagagaa
    15901 agcttgtggt cggagatgaa agccgtcgtg gggttggcag tccagcccag cagatcggat
    15961 tcgaagtcgc tgttggtcag caggtcataa ccggcaaggg gagccagcag cacctgatca
    16021 atcaggatag cgtcagaaga agtaccctgt cccttgctga acgagattct ggcatagacg
    16081 gcctgagcag gaacctgatc ggtcaaagca aagaatgtga gacgggcagt gttggtgatg
    16141 cggccatcag gaacaaacat acgcagaccg atgccgattt ggttgtactc tttgtccagc
    16201 cacacgattt gtgccgtcag ggtaccgtta taacagcttt cagcgccacc catagcattg
    16261 aagctgaaaa agaggcgacg gcaaacggcg ccatataata aatcaagttc ctgatgaaga
    16321 ctggccacgc ccgaacccat cacagccaca gcagtgcctt cgaacacatt cgtattgcca
    16381 atataaacgt tgttggtcca ccagtaggaa tattcttctt caaagctcgg attctgtacc
    16441 aggttaacgg cgcgcagaca ggacgatacg gagttggaat ccgtacgggc cggacgacag
    16501 ccccaagagg cataattggc cgtcaacgca gatggattct gcatgccccg ggcagcattg
    16561 ccggaattgt tggccccatt attcttggat gcaaagcagc ccgaagatgg cctggatgtg
    16621 tgaaactgat ttttcataag atgattcacc ctttcgtaga atatatttcc atcttatgaa
    16681 tttcgatctc atcgtgtgac aacgacatat attcagaaaa ttccataagt taatacgcct
    16741 gacgcattaa cttatggaat tttctgaata tgcggcaagg tgctgcggtg gcagtggatg
    16801 attcaaaaat gtgtcaagaa tttcttgtga aggatggggg aggggacggc aacggtagaa
    16861 cagcaattag ataaaatgct gggaattgcg gggaaacaat ctaacaatgg gcgggcagtt
    16921 gttgcgcagt ggaccgccgc tttatcttgg tatggaattt gcattgttta tgggcagtgg
    16981 ctggactggg tttttggcca cagtacagag caatattacg aaaaaaaatt aatgataaaa
    17041 gcttaacagg agggagacgc ggacaatgaa gtggcaggag tactatgaca gtcataagac
    17101 cacggcggaa gaagcggtaa aagtgattca atcaggggac cgggtcgtat tgggacatgc
    17161 ctgtggcgag ccgcagctga tcgttgatgc actggttgcc agagcggatg aactggaagg
    17221 ggtggagatt gcccatgaag tgccgatggg caagggaacc tactgcctcc ctcagtatgc
    17281 cagaagtttc cggcataatg ccttgttttc cggtaaaccc agccgccagg ccattcgcga
    17341 gggacgggct gattatacct ctatctattt acatgaatta cccattcttt ttaccagcgg
    17401 acagtttccc atcgatgtcg ccatggttac cgtctcaccg cccgacgagc ttggacgggt
    17461 cagcctgggc atatcagtgg attatacctt gcaggccata aaaagtgcta aagc
//
LOCUS       DAOTHZ010000046        17017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4058_length_17017_cov_7.391227, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000046 DAOTHZ010000000
VERSION     DAOTHZ010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 17017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17017
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4058_length_17017_cov_7.391227"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="P5549_06665"
     CDS             complement(<1..513)
                     /locus_tag="P5549_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HRW12791.1"
                     /translation="MEKNKSCFIAHFREEDNKEQSLWEHLDGTSKLASKNASKIGLEK
                     SGELIGLMHDIGKATELFDQYIRSAVGLIDPDEDDYLSVDGYQGKIDHSSAGAQLIYN
                     YLEPKGGQESIAAQFLALSVASHHSGIIDCLTPGGMNKYEARMKKAFELTRVDEAKAN
                     LDSTINDRIYA"
     gene            1012..1644
                     /locus_tag="P5549_06670"
     CDS             1012..1644
                     /locus_tag="P5549_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRW12792.1"
                     /translation="MAQAEKISCQKRDIKTQGYLNELKRNGMVPGTVYGKGVDNQAIS
                     VSLPQLARTFHAHGSRGLFSLEIDGGNGLMVVVREIQRHPITGQMTHVDFWKVKMDEK
                     IHNTVGISIVGEEEIMKKGGVLQAGAKEIEVFCLPQDIPETLVYDVAALEIGDKVTVA
                     DLQLPAEVEILTDLETMLATVLAPSKASAGEEAEEAQATETQVTAATEGE"
     gene            complement(1895..1979)
                     /locus_tag="P5549_06675"
     tRNA            complement(1895..1979)
                     /locus_tag="P5549_06675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Leu,seq:gag)
     gene            complement(2124..3737)
                     /locus_tag="P5549_06680"
     CDS             complement(2124..3737)
                     /locus_tag="P5549_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW12793.1"
                     /translation="MRRVNVRILALAALTLFMAAGGAIMMQKALYTNSLSTRPFTVGI
                     TEPVEKLEPASLQNHSQHLLATAIYEGLVCYNEARQSLEPRLARKWQYSPDGKSVTIF
                     LRKDVKFSNGKRLTAQDVKASFEYNISHAAEWSNTCLYLPIAGSKEKINGKSPEVTGI
                     QVLDKSSLKITLQQPHAAFMASLSNPIFWVMDLESGLTPPAGTGPYILHEQKAQSLQL
                     LRNDQYYRGQPRLSALHVTIYQDQETAFKDFQQGKLDLLNAVPAAEVPGIRQSKDKNL
                     LLEKPVLETYCLGFNLQRDPYVRSYLLRRALNYAIDREQIIKQMPNGAYLPAKGLIPA
                     GLAGYNKDIYGYRYDADKARQLLAEAGYPDGQGLPVLTLTYNDDPGHALVAEEVARQL
                     GQFGIRVQTQQQSWDYFKKEVNNRSLSFFRLSWAADYPDADSYLYSLFHSSQIGMTNY
                     CGYKNPQVDKILNDSRQQFEDQQARLALLQRAEAMILDDAPCLWLFQKKAQKLIGPEV
                     RNLKLDRMEMIDWLQVELHRPTIEKASED"
     gene            complement(3926..4486)
                     /locus_tag="P5549_06685"
     CDS             complement(3926..4486)
                     /locus_tag="P5549_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRW12794.1"
                     /translation="MPKGRSRLSTILWVTILLFLAVVLSFPKWITVFYPLPHQELIFR
                     EAKEHNVDPYLVFAIIRAESKYQNAAESSVGAKGLMQIMPETANWIAEQNGIEDFTLQ
                     ELHQPEVNIRFGCWYLHNLSAEFDGNVPLTVASYNAGRGKVQEWLVGGTWNGEPEDID
                     RIPFPETRQYTRNVLKNYEAYQAIYE"
     gene            complement(4520..5128)
                     /gene="coaE"
                     /locus_tag="P5549_06690"
     CDS             complement(4520..5128)
                     /gene="coaE"
                     /locus_tag="P5549_06690"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641401.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRW12795.1"
                     /translation="MKVIGLTGGIASGKSTVSKTLKGLGAYIIDADETAHKVVEPHKP
                     AWNDMVTAFGQDILNPDETINRDKLGEMVFGNSEQLTRLNHITHPRIMESFKEDYRRI
                     KAENPEAILVFEVPLLYETFMDRMCDEVWVVWVDYETQLKRLMDRNHYSPEEAKRRIE
                     SQIPLDEKAHRADVVINNCGSIEETINSATKFFNTILERGKI"
     gene            complement(5148..5783)
                     /gene="ytaF"
                     /locus_tag="P5549_06695"
     CDS             complement(5148..5783)
                     /gene="ytaF"
                     /locus_tag="P5549_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HRW12796.1"
                     /translation="MDLLAIMLFALAVSGDGLMVGMAYGIRKIKIPCTSLLIIALASA
                     LAVSVSMLLGKGLGMLISPQQAKTAGAALIFAIGVYFLLQAARQKIMHLQQSEEDPLL
                     TLHCNALGIIIQILKKPASADFDCSGEISLREAFFLGLALAMDALGAGAGLALAGMNI
                     FFTATSVGMVKFILVNTGMRMGQKMNDERWQSLAPFLTGFIFVAVAMIEYM"
     gene            complement(5867..6673)
                     /gene="mutM"
                     /locus_tag="P5549_06700"
     CDS             complement(5867..6673)
                     /gene="mutM"
                     /locus_tag="P5549_06700"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRW12797.1"
                     /translation="MPELPEVETIRRSLLGNIGAVIQDIELRRTDIIKRQDYEPTGAI
                     GQTISAIRRRGKYLIVDLDAGYTLVVHLGMSGRFYQENEEIEVSDRHVHMMIHLNNGH
                     KLIYMDPRRFGGVRFLCDPECIFCRMGVEPFSPAFTAQYLREICRDRKVAIKTLLLNQ
                     QCIAGIGNIYADEALFAARIRPDRPAGSLRLAEIKRLRSSIVQVMQASIDQGGTTFRD
                     FRNGYNKSGEFQKMLQVYGKTDGACNTCGKAIKKVVIGGRSAHFCEHCQK"
     gene            complement(6676..6831)
                     /locus_tag="P5549_06705"
     CDS             complement(6676..6831)
                     /locus_tag="P5549_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12798.1"
                     /translation="MESKCATCPMRIAAEKNHRTFRARLWFWHTKWCPGWKAYQRELA
                     QQENQGV"
     gene            complement(6855..9476)
                     /gene="polA"
                     /locus_tag="P5549_06710"
     CDS             complement(6855..9476)
                     /gene="polA"
                     /locus_tag="P5549_06710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRW12799.1"
                     /translation="MKDNTLLLIDGNSLLFKAFYALPLLHTREGIYTNGVYGFLTMFN
                     RVSADVQPSHVVVAFDMDRKTFRNETYSDYKANRSAAPDELVGQFHLLREVLAAMNVS
                     YVEMQGFEADDLIGTLSQQAEAAGMETIILTGDGDALQLVSEHTKVYMSKKGITDMQV
                     YDPQAVVDKWEVPPEQMIEIKALMGDSSDNIPGVPGVGSKTAIKLIKAYGSLENLYEH
                     IDEISGKKLKENLLTYRDQAYLSRDLATIRREVEMGAALDDFAVREMQRAELSALFKK
                     LEFKGLLAALEQSRSGVEEQELPGMKEPLPVLAVRELKSSAEVDEFLKPLEQQALALH
                     FETNYHHPMWAQGIKVYLAAGQQLAWIDLQNSAALEWLRAWLEDQTQPKYLHNAKFAQ
                     VFLLRAGIHLRGIKGDSLLLSYVLDPSFQGETLVEIIAHHMKLDTNHMDLAAQVVTIV
                     PLHQQMEEELTDALRCLLYDVEIPLSEILAAMEFAGIKVEKRVLAQLSEELSAGLDRD
                     EDRIYALAGVKFNINSPKQLAQVLFEDLQLPGGKKTKSGYSTGQEVLENLLDQHEIIP
                     YIMDYRQLSKLKSTYTDALQNLIHPETGRIHTIFKQALTATGRLSSVEPNVQNIPIRM
                     EAGRRVRKAFTAGSKETLLFTADYSQIDLRALAHISGDETLIDTFQRGIDIHTRTASE
                     IFKVPLERVDENLRRRAKAVNFGIIYGISDFGLARDTGVTRKEAGRYIEEYLDSYPGV
                     KKYMQEIVEFGIQNGYVETALGRRRYLPDLRARNKMVQSFARRMALNTPIQGTSADII
                     KLAMIKVADMLQTHHLQGRLLLQVHDELLLEVPRSELEETADIVKNCMENAFALKVPL
                     LVSMKVGTNWYEMQSWE"
     gene            complement(9573..10619)
                     /locus_tag="P5549_06715"
     CDS             complement(9573..10619)
                     /locus_tag="P5549_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRW12800.1"
                     /translation="MNCEQMQRLFSPYLDKMTTATENEAIAEHLGECAACAQQMQEMD
                     RMCALLKNLDTPPLPVDFAEKLHERISHEKIQLFPVIKPTTPKRTGWVAAAVAGLAVS
                     AGIFASSYLPYGAMVASLQDWMNKEDRPRVAIVDNNKILQEWINKQLEPSPEVDAPTT
                     EPAGQTGVQKPGTIKPGESIGIAPAVTKPVEVAVQERVEQNYTAKIQVDDMDQSMQDV
                     MQLAYASGAQISVKSANVMAADDNVKVVTLQVPKDKANEMLNQLGSVGVEAPLQNEVT
                     HTQAYHENQKALAGLEMDIENLQNSGSLSEEQQSQLQALLQQQQELQAQQEKIDKAVN
                     AVTIEVRLLEKTNP"
     gene            complement(10652..11233)
                     /locus_tag="P5549_06720"
     CDS             complement(10652..11233)
                     /locus_tag="P5549_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRW12801.1"
                     /translation="MNSDQLLVKRTLRGDTYAFDELVKTYQNKVYMLAYRYMGNEDDA
                     NDMAQESFIKAFRSLRSFKGDASFGTWIYRITTNVCLDELRRRKRKIVPLSLDEPLAT
                     LDGDEFEREIADTSLAADVVYERKEFTQNMQLLLDEMRPEHKAVIVLRDVMDLSYEEI
                     AIVLDCSIGTVKSRISRARIILQKKLIERELLK"
     gene            11517..11601
                     /locus_tag="P5549_06725"
     tRNA            11517..11601
                     /locus_tag="P5549_06725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11551..11553,aa:Leu,seq:caa)
     gene            complement(11810..13759)
                     /locus_tag="P5549_06730"
     CDS             complement(11810..13759)
                     /locus_tag="P5549_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12802.1"
                     /translation="MALKTVPDIDFRIQWLDLWHDYIHTVPQMFYHPVKHHGDRPAHM
                     FWQDDKWVTITYKEFAAVAEEIAAGLMGLGVAKGDNLVIMSVSCAEWGWADMGIELAG
                     GCTTTIFPSLSKDDVKFIVNHSQVNYLFAGSPALAEMVNSLREEQPTLKGIIVLKELA
                     AGNGKDLFTLDEIRASGRQLLQGQPSLVADRTAAITPQDAATTVYTSGTTGKLKAVRL
                     KHGEVTDGIWRCLRNQMMGEIGVYTHDTYLSVMPMAHIMERTYGMFCIMAVGSCLAWG
                     RGPAYVIQDMQETRPTFMVMVPRMMDRILKGLRQVFGATPEGAKAWDWAIDVACRAVD
                     ARIGEDETFDMSLDYLDELSGDIREEYVKAKAMVFDKVNAAMGGRLRCFASGGAAILP
                     ELHKPFLGMGFFVANGYGLTETLCGVGVGRGTCVKVSWNAPIAPGIEWRQEEDGELLI
                     KGAGIIQEYYNDPESNQDCFTEDGFFRTGDIVEFDKNGIMRVVDRKKAIIVMDTGKNV
                     APAKAESLLMRNILIDQVLIVGDGQKYITALICPNWDEIAKIADAKGIAYNKEDLKYD
                     LVNGLNTCVEVGPELYEAEFVRQIIAETVNQANSELADFEQIKKFRVPHRKFLQKYDE
                     LTPTSKAKQRVIMANFAGDIAALYQ"
     gene            13836..14951
                     /locus_tag="P5549_06735"
                     /pseudo
     CDS             13836..14951
                     /locus_tag="P5549_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            14991..15263
                     /locus_tag="P5549_06740"
     CDS             14991..15263
                     /locus_tag="P5549_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12803.1"
                     /translation="MEEVFYEHPAVDKVAVIGLPDPKRGENVGVFLTLKADHSATAED
                     LMAFCQPKLAKYKWPSVIEIRDSLPESNVGKLLKKELRSEVLQRFQ"
     gene            15414..16997
                     /locus_tag="P5549_06745"
     CDS             15414..16997
                     /locus_tag="P5549_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRW12804.1"
                     /translation="MPALSEESLREVKRRRTFAIISHPDAGKTTLTEKLLLFGGAIRM
                     AGEVKGRKAQRFARSDWMEIEKERGISVTTSAMQFDYGGYRINILDTPGHQDFSEDTY
                     RTLTAADSAVMLLDAAKGVETQTIKLFKVCSQRGIPIFTFINKMDRYGKEPLALLEEL
                     EQVLGIRSCPMNWPIGMGDEFSGIYDRQHHRIELYRGDSQRVFFHPKAKGLDDPAIRE
                     LLGDGLYEKLCEDVDLLDIAGDPFDFEKVKQGQLTPVYFGSAINKFGVQTFLDEFISL
                     APAPSAHESSVGPVDTASAFSGFVFKIQANMNPAHRDRIAFLRVCSGRFERGMAVNHV
                     RTGKKIKLAQPQQFLAQDRDIVEVAWPGDIIGLHDPGIYRIGDTLCEDGVFTFPPLPQ
                     FPPEHFARVSNLNAMKYKPFQKGVSQLEEEGTVQVFHTADHAEEIILGVVGELQFEVF
                     KYRLKGEYGADIRIQHLPYQLARWVTSEAAFDPGKLSNLSCLCVFDRDERPVVLCEHD
                     YSLNWLERTYSDLIFSVTP"
BASE COUNT         3863 a         4446 c         4249 g         4459 t
ORIGIN      
        1 tgcgtatatt ctatcgttga tggtactatc gagattagct ttcgcctcat cgactcttgt
       61 taattcaaag gcctttttca ttctcgcttc atatttgttc atcccacccg gcgtcagaca
      121 atcgattatt ccggagtgat gagaagcgac agacagtgcc agaaattggg ccgcaatact
      181 ctcctgtcct cctttaggct ccagatagtt ataaatcaac tgggcacccg cactggaatg
      241 atcgattttt ccctgataac cgtctacact taaataatca tcttcatcag gatcaattaa
      301 tcctaccgct gaacgaatat attgatcgaa caattcggtg gctttaccta tatcatgcat
      361 tagcccaatc aactcaccag atttttcaag cccgattttt gatgcgtttt tagaagctaa
      421 cttggaagta ccatcaagat gctcccatag agattgttct ttgttgtctt cttctctaaa
      481 atgagcgata aagcaggatt tatttttctc cattatcaat accaccctga ttattattta
      541 ttcgttacac tttgttactt tccattatat ccctaaactg tttgagtttc tagaaaatat
      601 gatttaattt gctattatct tccaccgtta ttgccatttg ggttgagttt gtttttatta
      661 aatatacttg tccccaccat acccaaaatc agtgacaata ctctgtcgct gcttccgttg
      721 taatattttt ttcatgcatc atgcaaatgt gtaacttatt taaagcaaac tcccaaaaat
      781 tctatgaccc attcttcgaa caactcttgc cgacgcaagt tggggacggt tcttgccttg
      841 cgcgtttttg gttcctgacc gcgcaaggca agaaccgtcc ccaacttgcg tcgaaggcct
      901 gtccataagc tgaaggttgg cttttttaga catgatgttg gcaaactggt ataattagta
      961 cttgaaagaa taaacaacct ggttcaacag gttgatgaag gagtggtagt aatggctcag
     1021 gcagaaaaaa taagctgcca gaaacgcgat ataaaaaccc agggctattt aaatgaacta
     1081 aagcgtaatg gcatggttcc cggaacggta tatggcaaag gggtggacaa tcaggccata
     1141 tctgtaagcc ttccgcagct tgcccgtacc ttccatgctc atggctcgag ggggctgttc
     1201 tcattggaaa tcgatggagg caatggctta atggtggtgg ttcgagaaat acagagacat
     1261 cctattaccg ggcagatgac ccatgttgac ttctggaagg taaagatgga tgagaaaata
     1321 cacaataccg tggggatttc cattgttggt gaagaagaga ttatgaaaaa agggggtgtc
     1381 ctgcaagcag gcgctaaaga aatcgaggtt ttctgcctgc cccaggatat tccggaaacc
     1441 ctggtctatg atgtagctgc tctggaaatt ggagacaagg ttacggttgc cgatctgcaa
     1501 ttacccgcag aagtagaaat acttactgat ttggaaacaa tgctagccac tgtactggcg
     1561 ccatccaaag cgtctgcggg agaggaagct gaagaagccc aagccactga aacccaagtg
     1621 acagcagcaa ccgaaggcga ataagcaaac gaagcatgac cacccgctca gcgggtggtt
     1681 tgcattttct gacattctca gctttggcct gttctgccag attacgatgc aaagatttgg
     1741 cattcgaatg ttgcgtttac ccgaataatc accggaaatc ggctcaggaa ttatgagccc
     1801 atctgccaac gctgattcca accagcgaca aaaaaaagcc ttgcgtattt tgcattaatg
     1861 caaaatccac aaagccttgt gtttctataa tttatggtgc ggcgggaggg acttgaaccc
     1921 tcacgcctct cagcatacgc ccctcaaacg tacgtgtctg ccggttccac caccgccgca
     1981 tccggtcaga ttcagggggt ccttaacctg acaaaggaaa ttatacaaca gtcggctctt
     2041 tgatgtcaat caatctgctc ggctggcttg cttctcctgc cggtgcgggg cggtttgacc
     2101 tggagcaacc ggcattatgc tttttaatcc tcgctggctt tttcgatggt gggccggtgc
     2161 agttccacct gcagccagtc aatcatttcc atacgatcca gttttaaatt gcggacctct
     2221 ggaccgatca gcttttgagc tttcttctgg aacaaccaca aacacggggc atcatcaagg
     2281 atcattgctt cggcacgctg caacagggcc agccgggcct gctggtcttc aaattgttgg
     2341 cgggagtcat tcaggatctt atcgacctgg ggatttttat agccgcagta gttggtcatg
     2401 ccgatctggg aactgtggaa caagctgtac aggtagctgt cggcatcggg atagtctgcc
     2461 gcccagctca gccggaaaaa gctgagggaa cggttgttga cttctttctt gaaatagtcc
     2521 cagctttgct gctgggtctg tacgcgaatg ccaaactggc ccaactgtcg ggcaacttct
     2581 tctgccacca gggcatgacc gggatcgtcg ttgtaggtca gggtaagcac gggcagtccc
     2641 tgcccgtcag ggtatccggc ttcggcgagc agttggcggg ctttgtcggc atcataacga
     2701 tagccgtaaa tgtctttgtt atagcctgcc agtccggcgg ggatgagtcc tttggcagga
     2761 agataggctc cgttcggcat ttgtttgatg atttgctccc ggtcgatggc gtagttgagc
     2821 gcccggcgca gcagatagct gcggacataa ggatcgcgct gcaggttaaa gccgaggcag
     2881 taagtctcga gaaccggctt ttcgaggagc aggtttttat ccttgctctg acggataccg
     2941 ggcacctcgg ccgcgggtac ggcattcagc agatcaagct tgccttgttg gaagtccttg
     3001 aaggcggtct cttggtcttg atagatagtc acatgcagcg ctgacaggcg cggctggccg
     3061 cggtagtatt ggtcgttgcg caacagctgc aaggattgtg ccttttgttc gtgtaaaatg
     3121 tagggtccgg tcccggccgg gggggtgagt cctgactcca ggtccatcac ccagaaaatg
     3181 gggttgctga gtgaagccat aaaggctgca tggggctgct gcaaggtgat tttcaggctg
     3241 cttttgtcga gcacctggat gcctgtcact tccgggcttt tgccatttat tttttctttg
     3301 ctgccggcaa tgggcagata cagacaggta ttgctccatt cggcggcatg actgatattg
     3361 tattcaaagg aagctttcac gtcctgggcg gtgagtcgct tgccgttgct gaacttgacg
     3421 tccttgcgca aaaatatcgt gaccgatttg ccgtctggac tgtattgcca ttttcgggcc
     3481 aggcgcggtt caagactttg ccgggcttca ttataacaga ccaggccttc gtaaatagcg
     3541 gttgccagca ggtgttggct gtgattttgg aggctggctg gctccagttt ttccaccggt
     3601 tcggtgatgc cgacggtaaa gggtctggtg ctgagactgt tggtatacag ggctttttgc
     3661 atcatgattg ctccgcctgc cgccatgaac agggtcaggg cagccagagc aaggattcgg
     3721 acattgacac ggcgcataat ttgtccctcc ttttttatcc atgggacatt ttatgcgttc
     3781 acttggctga ctatgcctgc gatgccggca ggatccattc tgggaaaaaa aacaaccggc
     3841 ccgggccggt ctcagcgtga aatgtgaatc gttgtctggt caagaacctg cggctctgcc
     3901 tcaggatgac attcttttaa attcttcatt catatatggc ctgataggcc tcatagtttt
     3961 ttaaaacatt cctggtatac tgacgggttt ccgggaaagg gatcctgtcg atgtcttcag
     4021 gttctccgtt ccaggtgccg cccaccagcc attcctgcac tttgccgcgc ccggcattgt
     4081 agctggcgac agtcaggggc acattgccgt cgaattccgc actcaaattg tgcagatacc
     4141 agcagccgaa tcgaatgttg acttccggct ggtggagttc ctgcaaggtg aaatcctcga
     4201 ttccgttctg ttctgcaatc cagttggcgg tttcgggcat gatctgcatt aatcctttgg
     4261 cgccaacgga agattcagcc gcattctggt atttgctctc cgcacggatg atggcgaata
     4321 ccagataggg gtccacattg tgctctttcg cttcgcggaa aatcaactcc tgatggggca
     4381 acggataaaa gaccgtgatc catttgggga agctcagtac gactgccaga aatagcagta
     4441 ttgtgaccca aagtatggtg gataaacgac ttcttccttt cggcaccaat gttaaacccc
     4501 caagaaatga ttttttacat catatttttc cccgctccag aatagtattg aaaaatttgg
     4561 ttgccgagtt gattgtttcc tcaatactgc cgcaattgtt tatcaccaca tcagcccgat
     4621 gggctttttc atccagggga atctggctct ctatacggcg tttggcttct tcgggactgt
     4681 aatgattacg gtccatcagg cgcttgagct gggtttcgta atctacccag acgacccaga
     4741 cctcgtcgca catccgatcc ataaaggtct catacagcaa gggtacttcg aacaccaaga
     4801 tggcctctgg attctcggct ttgatccttc tgtagtcttc tttgaatgat tccataatgc
     4861 gtggatgggt aatgtgattg agtcgggtca gctgttcaga attgccaaag accatctcgc
     4921 ccaacttgtc ccgattgatg gtttcatcgg ggttgagaat gtcttggcca aaagcagtaa
     4981 ccatatcatt ccaggcaggt ttgtgtggtt cgacgacttt atgtgcagtt tcgtcagcat
     5041 caataatata ggcacccaac cctttgagcg tcttggaaac agtacttttt ccgctggcta
     5101 ttccgccggt caatccaatc actttcatcg taaagtatca ccaactttta catgtattcg
     5161 atcattgcca ccgccacaaa aataaaacct gtcaggaacg gcgccagact ttgccatcgt
     5221 tcatcgttca tcttctgacc cattcgcatg ccggtattta ctaaaataaa ttttaccata
     5281 cctacactgg ttgcagtaaa gaaaatattc atccccgcca gcgccagacc tgcacctgct
     5341 ccgagcgcat ccatggccag cgccagaccc agaaagaatg cttcgcgcag actgatctcc
     5401 ccggagcaat cgaagtcggc actggctggt tttttcagta tctgtataat aatgcccaat
     5461 gcattgcaat gcaatgtcag caggggatct tcttcagatt gctgtaaatg catgatcttt
     5521 tgccgggcgg cctgcaaaag gaagtagacg ccgatggcaa agatcagggc agcgcccgcc
     5581 gttttggctt gctggggaga gattagcatc cccagacctt ttcccagcaa catggagaca
     5641 ctgaccgcca gagcagaagc aagggcgatg atcaacaagg acgtgcaggg gattttgatt
     5701 tttctgatgc cgtaggccat gcccaccatc agtccgtctc cgcttactgc cagggcaaac
     5761 agcatgattg ccagaaggtc caccttgcca ctccattcat gtatcaaatc tatccatact
     5821 atatgaatgt tatggtcaag gtgtgaatcg cctgccggac ggtatcctat ttttggcagt
     5881 gctcgcagaa gtgagcgctg cgcccgccga ttaccacttt cttgatggcc tttccgcaag
     5941 tattgcaggc accgtcggtt ttaccgtaaa cctgcagcat cttttgaaat tctccgcttt
     6001 tattgtatcc gttccgaaaa tcgcgaaagg tggtaccgcc ttggtcaatg ctggcttgca
     6061 tgacttggac gatgctgctg cgcaagcgtt tgatctctgc cagacgcagc gaaccggccg
     6121 gccggtcggg acgaatacgg gctgcaaaca gggcttcatc ggcatagatg ttgccgatcc
     6181 cggcgatgca ttgttgattg agcagcaaag tcttgatggc gactttgcgg tcacggcaga
     6241 tctcccgcag atattgggct gtaaacgccg gcgagaaagg ctcaacgccc atgcggcaaa
     6301 agatacattc cggatcacac aagaagcgta ctcccccaaa tcgcctggga tccatataaa
     6361 tcagcttgtg tccattgttc agatggatca tcatgtgcac gtgacggtcg gagacttcaa
     6421 tctcctcatt ctcctggtaa aatcgtccgc tcatccccag gtgcactacc aatgtgtacc
     6481 cggcgtcgag atccacaatg agatatttgc cccgtctgcg gatggcgctg atggtttgcc
     6541 cgatggctcc ggttggctca tagtcctggc gtttgatgat atccgtccgg cgcaattcga
     6601 tgtcttgtat aacagccccg atgttcccca gcaggctgcg tctgattgtt tccacttcgg
     6661 gtagttcggg catgcttaaa ccccctgatt ttcttgttgg gccagttcac gctgatacgc
     6721 tttccagcct ggacaccatt tggtgtgcca gaaccacaag cgcgccctga aggtgcggtg
     6781 attcttttcg gcggcgatgc gcatgggaca ggttgcacat ttggattcca tcctgctgcc
     6841 tcctttctca ctttttactc ccaggactgc atttcatacc agttggtgcc caccttcatg
     6901 ctgacgagca aaggcacctt tagtgcaaac gcattctcca tacagttctt gacgatgtcg
     6961 gcggtctcct ccaactcgct gcgcggcacc tccagcagca gttcatcgtg gacctgcagc
     7021 agcaagcggc cctgcagatg atgggtttgg agcatgtcgg ccactttgat catggccagt
     7081 ttaatgatgt cggctgacgt tccctggatg ggcgtattca gggccattct gcgggcaaag
     7141 gactgcacca ttttatttct ggcgcgcaga tcaggcagat aacgtctccg tcccagagcg
     7201 gtttccacat agccgttttg aatgccaaac tcgacaatct cctgcatata tttttttacg
     7261 cccggatagg agtcaagata ttcttcgata tagcgtcccg cctctttgcg ggtcacgccg
     7321 gtatcacggg ccagtccgaa atcgctgatc ccgtagataa tgccaaagtt gaccgccttg
     7381 gcgcgccggc gcaggttctc atccactctt tccaacggca ctttaaaaat ctcggaagcc
     7441 gtgcgggtat gaatgtcaat gccccgctgg aaagtgtcaa tcagagtctc gtcgccgctg
     7501 atgtgcgcca gggcgcgcaa atcgatctgg gaatagtccg ccgtaaacag cagggtttct
     7561 ttggaaccgg ctgtaaaagc cttgcgcacc cgacgtcctg cttccatgcg aatgggaata
     7621 ttctgtacgt tgggctcgac actggacaag cgtccggtcg ccgtcagcgc ttgtttgaag
     7681 atggtatgaa tccggccggt ctcaggatgg atcaggtttt gcaaggcatc ggtataggtc
     7741 gacttcagct tgctgagctg ccggtaatcc atgatgtaag ggatgatctc atgttgatcg
     7801 agcaggtttt ccaagacttc ctgaccagtc gagtagccgc tcttggtttt tttgccgccg
     7861 ggcagttgga ggtcctcgaa cagcacctgg gccagctgtt tgggggaatt gatattgaat
     7921 ttgaccccgg ccagtgcata gatgcggtct tcatcccggt ccagacccgc actcagttct
     7981 tccgacaact gagccagaac ccgcttttcc actttgatgc cggcaaactc catggctgca
     8041 aggatctccg acaacggtat ctccacgtca tagagcagac atcgcagggc atcggtgagt
     8101 tcttcttcca tctgctgatg gagcggaaca atcgtgacaa cctgcgccgc cagatccatg
     8161 tgattggtat ccagcttcat atgatgggcg atgatctcca ccagcgtttc cccttgaaaa
     8221 gagggatcca gcacataact gagcaacagg ctgtctccct tgatgccgcg cagatggatg
     8281 cccgcgcgca gcaggaatac ctgggcaaac ttggcattat gcagatattt gggctgcgtt
     8341 tgatcttcca gccaggcgcg cagccattcc agcgcggcac tgttctgcaa atcaatccag
     8401 gccagttgct gaccggccgc cagataaacc ttgattccct gcgcccacat gggatgatga
     8461 tagttggttt caaaatgcag ggccaatgcc tgttgctcca ggggcttgag aaattcatca
     8521 acttcggcag atgattttaa ttctcgtaca gccaagaccg gaagcggttc cttcatcccg
     8581 ggcagttcct gttcctccac cccggaccgg ctttgctcca gggcggccag cagccctttg
     8641 aactccagtt ttttaaacag tgcactgagc tcggctcgtt gcatctcccg gacagcgaag
     8701 tcatccaggg ctgcacccat ctccacctct cgccggatgg tcgccagatc acggctcaaa
     8761 tacgcctgat cccgataggt cagcaggttt tctttgagtt tttttccgct gatttcgtcg
     8821 atgtgttcat agaggttttc cagactgccg tatgctttga tgagcttgat ggcggtttta
     8881 ctgccgacgc ccggcacacc cgggatattg tcagagctgt cacccatcag ggctttgatt
     8941 tcaatcatct gctccggcgg gacttcccat ttgtcgacca ccgcctgcgg atcatagacc
     9001 tgcatgtcgg tgatcccttt cttgctcata tataccttgg tatgttcgct gaccagctgc
     9061 agcgcatccc cgtctccggt caggatgatg gtttccatcc cagccgcttc agcctgttgg
     9121 ctcagggtgc cgatcaggtc gtcggcttca aatccctgca tttccacata actgacattc
     9181 atggcggcca gcacctcacg cagcaaatga aactgaccga ccagttcatc cggggccgcc
     9241 gaccggttgg ctttgtaatc actgtaggtt tcattgcgaa aggttttccg gtccatatca
     9301 aatgccacga ccacgtggga aggctgtacg tccgcactca cccggttgaa catggttaaa
     9361 aaaccatata ccccgttggt atagatccct tcccgggtat gcagcagggg cagcgcataa
     9421 aacgctttaa aaagaaggct gttcccgtct atcagcagca gggtgttgtc tttcatggcc
     9481 gacctccagc aaaattattt cagaaaaaac aaaatgggca gaaatatgtt cctgcccatt
     9541 attatagctt atattcaatc atttatttag tctcaaggat ttgtcttttc cagcaagcgc
     9601 acttcaatgg tgactgcatt gacggctttg tcaatcttct cctgttgcgc ctgcagttcc
     9661 tgttgctgct gcaacagggc ttgcagctgg gactgctgct cctcactgag gctgccgctg
     9721 ttctggagat tctcaatgtc catctccaat cccgccaagg ccttttggtt ctcatgatag
     9781 gcctgggtgt gggtcacttc gttttgcagg ggcgcttcca cgcccacact gcccaactgg
     9841 ttgagcattt cgttggcttt gtctttggga acctgcaggg tcactacttt cacattgtca
     9901 tccgccgcca tcacattggc agacttgacg ctgatctggg caccgctggc ataggccagc
     9961 tgcatgacat cctgcatcga ttggtccata tcgtcaacct gtatcttggc ggtatagttt
    10021 tgttccactc tctcctgcac agcgacttcc accggtttgg ttactgccgg tgcaatgcca
    10081 atgctttctc ccggcttgat tgtgccaggc ttttgcactc cggtttggcc ggccggttcg
    10141 gtggttggtg cgtcaacttc cggcgacggt tcgagttgtt tgttgatcca ttcctgcaaa
    10201 attttattat tgtcaacgat cgctacgcgc ggtctgtctt ctttgttcat ccaatcctgc
    10261 aatgacgcta ccattgctcc gtagggcaga tagctgctgg caaatatacc ggcactcacg
    10321 gccagtcctg ccaccgctgc agccacccag ccggtacgtt tgggggtcgt tggcttaata
    10381 acggggaaca gctgtatctt ttcatgactg atacgttcat ggagtttctc ggcaaaatca
    10441 accggaagcg gaggcgtatc cagatttttg agcaaagcac acatacggtc catctcttgc
    10501 atttgctgtg cacaagcagc acattcgccg agatgttccg cgatcgcttc attttcagtt
    10561 gcggtggtca ttttgtccag gtatggagaa aataaccttt gcatttgctc gcagttcatg
    10621 atgctctcct cccgcttttt tagacgaggc tctactttaa aagttcccgt tcgatcaatt
    10681 ttttttgtaa aatgatccgt gccctgctaa tcctcgattt tacggttccg atggagcagt
    10741 ccagcacgat tgcgatttct tcgtaactca aatccatgac atcccgcagc acaatgactg
    10801 ctttgtgttc aggcctcatc tcatccaaaa gcagctgcat attttgcgta aattccttgc
    10861 gttcatagac cacatcagcc gccagggagg tgtcggcaat ttcccgttca aattcgtccc
    10921 cgtccagcgt agccaagggt tcatccaggg aaagcggtac gattttgcgt ttgcggcggc
    10981 gcagctcatc caggcagaca ttggttgtga tgcggtaaat ccaggtgcca aaactggcat
    11041 cgcctttgaa ggagcgcagc gaacggaagg ctttgataaa tgactcctgc gccatgtcat
    11101 tggcgtcatc ctcattgccc atatagcggt aggccagcat atatacttta ttttgatatg
    11161 ttttcaccaa ttcatcgaaa gcgtatgtat ccccacgcag ggttcgtttc accagtagtt
    11221 gatccgaatt catcagtatc tcctccgggc cagctttcct ccggtcagct gcagcaaaac
    11281 agcccccctg ctggtcccgc catgtaaata cgtcctaagt atggttacta ttctgcacgt
    11341 ttaaagaata ttcctttaaa aaaggcctgt tcctgcttat gtaaagtatg taaccgatgc
    11401 cataatatag ctgtcaaggt ccggttttcc cttggccagg cggcgatggt gtggttcttg
    11461 acaaccagga tggcaaacct tataataatt cttgcgtccg cacaaaacaa tattaagcca
    11521 gagtgtcgga attggcagac gagcgcgact caaaatcgtg tgccgcgagg cgtgtgggtt
    11581 cgagtcccac ctctggcacc atcagaaaaa cagggctttg cgaagccctt ttcttgtttt
    11641 ctgaggtttc attataccat atgtgcaaca ggatggttgg cttcggcgca gaataaacgc
    11701 aggtatattt tatgaaagta ttatgaagat tggatgaaga agtcacccct gtcgataaaa
    11761 aaccaggcct cagcagcgag gctggggcct ggtccggtgg cggtgacgct tactgataaa
    11821 gggcagcaat atctcccgcg aaattggcca taatgacccg ttgtttggct ttggatgtag
    11881 gggtcagctc atcatatttc tgcaggaact tgcgatgcgg tacccggaac tttttgattt
    11941 gttcaaagtc agccagttca ctgttggcct ggttgactgt ctcggcaatg atttgccgta
    12001 caaattctgc ttcatataat tccggtccga cttccacgca ggtattcagt ccattgacca
    12061 gatcatattt taagtcttct ttattatagg caatcccttt ggcatcagcg atcttggcta
    12121 tttcatccca attgggacaa atcagcgcgg tgatgtattt ttggccgtct cccacgatca
    12181 gtacctgatc gatcaaaata ttgcgcatga gcaaggattc cgccttggcc ggtgccacgt
    12241 ttttaccggt atccatgacg atgatggctt tcttgcggtc cacgacccgc atgatgccgt
    12301 ttttatcaaa ttcaacgata tcaccggtcc tgaagaaacc gtcttccgta aagcaatcct
    12361 gattggattc cggatcattg taatattcct gaatgattcc ggcacctttt atcagcagtt
    12421 cgccgtcctc ttcctgacgc cattcgatcc ctggagcaat gggcgcattc cagcttacct
    12481 tcacgcaggt gccgcgcccc acgccaaccc cacaaagcgt ttcggtgagt ccatacccgt
    12541 tggcaacaaa aaatcccatg cccaggaagg gtttgtgcag ttctggcagg atggccgctc
    12601 cgccggaggc aaaacaacgc agccgtccgc ccatggccgc atttaccttg tcaaaaacca
    12661 tggctttggc tttgacgtat tcttcgcgaa tatcaccgct cagttcatcc agataatcga
    12721 gactcatatc aaaggtttca tcttcgccaa tgcgggcatc gaccgcccgg caggcaacat
    12781 cgatggccca atcccatgct ttggcccctt ccggggtagc cccgaacacc tggcgcagac
    12841 ctttcaggat ccgatccatc attctcggca ccataaccat gaaagtaggc cgggtctcct
    12901 gcatatcctg aatcacataa gcaggtccgc ggccccaggc cagacaggac cccaccgcca
    12961 tgatgcagaa catcccgtaa gtgcgttcca taatatgggc catgggcatg acggacaggt
    13021 aggtatcatg cgtataaacg ccgatttccc ccatcatctg gttgcgcaga caacgccaga
    13081 tcccatcggt cacctcaccg tgtttgagac gtaccgcttt tagttttccg gtggtcccgg
    13141 acgtataaac ggtggtggcg gcatcctgcg gcgttatggc agcagtccgg tcagccacca
    13201 gggaaggttg tccctgcagc agctggcgtc ccgatgcccg tatttcatcc agggtgaaga
    13261 ggtcttttcc gttgcccgcg gccaactctt taagaacaat gatgcctttc agggtgggct
    13321 gttcttcacg caaggaattg accatttcgg ccagcgcagg actgccggca aataaatagt
    13381 tgacctggga atggttgacg atgaatttga catcatcctt gcttagactc ggaaaaatag
    13441 tggtcgtgca tccgcctgcc agttcaatgc ccatatccgc ccagccccat tcggcgcagc
    13501 tgaccgacat gattaccaga ttgtctcctt tggcaacccc tagacccatc aaaccggcgg
    13561 cgatttcttc tgctactgcg gcaaactcct tgtaggtaat cgtaacccat ttgtcatcct
    13621 gccagaacat atgtgcaggc cgatccccat ggtgtttgac cggatgataa aacatctggg
    13681 gcacggtatg aatgtagtca tgccacagat ccaaccattg aatcctaaaa tcaatatccg
    13741 gtactgtctt taatgccatt atcattacct cctgcaaatt tcttggatgg ttggccaggc
    13801 tttcattcca gccataatac ggttcacaag acaaggtagc ggttttactt cccaactgca
    13861 tatcgatcgt ggtcgcctat tatgcgattc tgaaagcagg cggcgtcgcc gtgatgaaca
    13921 atcccattta tgcggatcgg gaactcgagt atcagttcat cgattccgag tccaaattgc
    13981 tcatttgtct ggatatgctg gtcccccgca tgctcaatat aaaaccccgt acaaaaattg
    14041 aaaaaatcat ttggacctcg atcaatgact acgccctgcc cggtacaact gacagcggtt
    14101 ttagcgtgcc ggatgcaccg gatcttttcc attacaagaa actcctcgag aaatatgaac
    14161 cgattcctcc ggcgatgcaa attgattggg aggacacagc ggtcctgcaa tatacagggg
    14221 gaaccaccgg tctggccaaa gcggcggtgc tgacccaaaa aaatctctgc accatgttgc
    14281 agcgctacga agcatggctg cacgatgggg taaaaggtca ggacacggat atgtcagctt
    14341 ccccgctgtt ccatattctc ggcatgcagg ttggcatgaa cttttccatt catatgggct
    14401 ggcgtaatgt actgctgccc agagccactc cagaagccat gctggaagcg attcgtaaat
    14461 atcgggtcaa ttattcccca ctggtgcctg cccactatat cggcatgctc cagcaccctg
    14521 atctgaccaa tacggatctc acttgtttta aagggctttt ctccggggga gcatcattgc
    14581 cggttgacat tttgcgacgc ttcgagaccg tctccaaagc agaaatttgc gagggattcg
    14641 gtatgtccga gacgtctccg cagacccacc tgaatcctta ccggggcggg gggccgcgca
    14701 aaccaggcag catcggcatt ccctggccgg atacggaagt tcggatcgtt gatttggaaa
    14761 ccggcgagat agacgtgccg atcggcgaac ccggtgagat gcttttccgc ggcccgcaag
    14821 tcacccggga atactataat aaaccggaag aaaccgcgat tgccattacc ccggatggat
    14881 ggctgcactc cggggatatt gcctatatgg atgaagacgg ctatttcttc attgtcgatc
    14941 gcaagaaaga cctcattatt tccagcggtt ttaacattta tccgcgggaa gtggaggagg
    15001 ttttctatga gcatcctgct gtcgacaagg tcgctgtaat cggtctgcct gatcccaagc
    15061 gcggcgaaaa tgtcggtgtg tttcttacct taaaagcaga tcacagcgcg acggcagaag
    15121 acttgatggc attctgtcag cctaaactgg ccaaatataa atggccctct gtcatagaaa
    15181 taagagattc actgccggag agcaatgtag ggaaattatt gaaaaaagaa ctgcgctctg
    15241 aagtcctgca gcgctttcaa taagctgctg atttgtattt taaatccaag agggaggctg
    15301 ctcgatggca gcctcctttc ttttattctc cagtccgcag gattgctttt gctcctgccg
    15361 tcatttgtga tatagtatta tttgttttta acatgcaggg aggattaatc gcattgccag
    15421 cactatctga agagagccta cgtgaagtaa aaagacggcg caccttcgcc atcatctccc
    15481 acccggacgc cgggaagacc acgctgactg aaaagctgtt gctgtttggc ggagcgattc
    15541 gcatggccgg cgaggtcaaa ggccgcaaag cccagcgatt tgcgcgctcg gactggatgg
    15601 aaatcgaaaa ggaacgcggc atctccgtca ccaccagcgc catgcagttt gactatggcg
    15661 gataccgcat caacatattg gatacaccag gccatcagga cttcagcgaa gacacctacc
    15721 gtaccctgac ggcggctgac agcgcggtga tgctgctgga tgcggccaaa ggggtggaga
    15781 cgcagaccat caaactgttt aaggtttgca gccagcgcgg aattccgatt tttactttta
    15841 ttaataaaat ggaccgttac ggcaaagagc cgctggcttt gctggaagaa ctggaacagg
    15901 tgttgggcat ccgctcctgt ccgatgaact ggcccatcgg catgggtgat gaattcagcg
    15961 gcatctatga ccgccagcac caccgcattg aactgtatcg cggcgacagt cagcgggttt
    16021 ttttccatcc caaagccaag gggctggatg atcctgccat ccgggaattg ctgggagacg
    16081 ggctgtatga gaaactctgt gaagacgttg atctgctgga cattgccggg gatccttttg
    16141 attttgaaaa ggtgaaacaa ggccagttga cgccggtcta ctttggcagt gccattaata
    16201 aattcggggt gcaaaccttc ctggacgaat tcatctccct ggcgcctgcc cccagtgcgc
    16261 atgaaagcag tgtcggaccg gtggatacag cttcggcttt ttctggtttt gtttttaaaa
    16321 tccaagccaa tatgaatcct gcccaccgcg accggatcgc attcctgcga gtttgctccg
    16381 gcaggttcga gcgcggcatg gcggtcaatc atgtccgcac cggcaagaag atcaagctgg
    16441 ctcagccaca gcaattcctg gcccaggatc gcgacatcgt ggaagtcgcc tggccgggag
    16501 acattatcgg tctgcatgat cccggcattt atcgcatcgg cgataccctg tgcgaggacg
    16561 gtgtttttac ctttccgcct ttgccgcagt ttccgccgga gcattttgcc cgggtatcca
    16621 acctgaacgc catgaagtac aaaccatttc agaaaggcgt cagccagttg gaggaagaag
    16681 gcaccgtgca ggtctttcac acagctgatc atgctgaaga aatcatcctg ggagtcgtgg
    16741 gtgaattgca gtttgaagtg tttaaatacc ggcttaaggg ggaatatgga gcggatattc
    16801 gcatccagca cctcccctat cagctggccc gctgggtcac atcggaagct gcctttgacc
    16861 ctggcaagct cagcaatctc agctgtctgt gtgtttttga ccgggatgaa cgtcccgtcg
    16921 tgctttgtga acatgattat tccctgaact ggctggagcg aacgtactcc gatctgattt
    16981 tctccgtcac tccataagct gcgagcaagt ggagcgc
//
LOCUS       DAOTHZ010000047        16488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4242_length_16488_cov_8.032982, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000047 DAOTHZ010000000
VERSION     DAOTHZ010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 16488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16488
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4242_length_16488_cov_8.032982"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="P5549_06750"
     CDS             complement(<1..241)
                     /locus_tag="P5549_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRW12805.1"
                     /translation="MKIYDELVARGLIAQVTDEEQIKELVNSGQAIFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPVVLIGGGTAYIGDPS"
     gene            complement(348..983)
                     /locus_tag="P5549_06755"
     CDS             complement(348..983)
                     /locus_tag="P5549_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRW12806.1"
                     /translation="MLAIFFKSLLIAFSGAVMPGSVFTYTVDRSLRHGVKAGVLVSLG
                     HALLELILVILIFVGAGRYLAAESAGIAIGLIGGVVLVYLGSQMLKDVYLNKITLETG
                     EMHDHKQGGMFMAGMVLSATNPYFLIWWSAVGLALIMSAYHSLGVWGIVIFYLGHILG
                     DITWYTFVAAMVSKSRHLLNIAIYKTIIVVLALCLVGFGASFFAGSIKHII"
     gene            complement(997..2670)
                     /locus_tag="P5549_06760"
     CDS             complement(997..2670)
                     /locus_tag="P5549_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRW12807.1"
                     /translation="MRQRSWAGIKRFFRVIRLFLHIFWSFYSLKIKAVWHADGWKEQR
                     RIELYVTEARLFRNTAVEMGGLLIKLGQFFSTRVDIFPQSVTRELANLQDEVPAVEFK
                     EMCMVLESEFGRPLHEIYPVLNPAPLASASLGQVHEAELFGGQKAAVKVLRPNIEKLI
                     DIDLRAVRQVIGWIQFFTDWEQWVDFDAIYREFEETLREELNYLQEGKNAETIAAHSV
                     ADKELIVPRIYWEYTRQRVLTMEFVDGYKVTDYAGLQQAGIDRSQLASRLLEIYIKQI
                     LVDGFFHADPHPGNLFIDPDGKVIMIDFGMVGTISPELKEKLIKMVLAMVDRDNLQVV
                     YYLKQVGFLRRDADDALIARAVGVFLEQLLGQGTDLFDNDLKSLLDDLEEILYEQPFQ
                     IPARFTFLGRALGTLYGICIGLDKNISFLDAAKPYLKQFMPEEARPLKIAKDKAAAWG
                     SALIEGPPLLAKVLRQAERGDLELKLPLKNLQENLEQNTRASLTIAWGIVLSAVMLST
                     ICLYVNGFTVESRWGAGATLIVGLLFISKTKSQSRKRVLHHPEGIKKTR"
     gene            2907..3746
                     /locus_tag="P5549_06765"
     CDS             2907..3746
                     /locus_tag="P5549_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12808.1"
                     /translation="MKQIATEQFTLHIIPGYIENLFLAEYQHGLLLLDCGAVSDVKRI
                     EDFCRRDLHRSPKDIRLAVVTHMHPDHAGGAVLLRDKFGIPLAGHQELDRWYAGPGGW
                     MQKVIDCLLMQSVAWRTRQQLEKGFFCSRIQPDFLLKDGDALPFFPDWQAVAVPGHTS
                     HDLVLYHADQSLLYVADCFLEVKGRYQTPLPVMFPQWLEASYQKMGALHPSSLLIAHG
                     EARRDQIPADLFSSLQHLLQTPPSKLTRRIKKLSVYTPEYHRQNKIFQQTWRKNRRRR
                     NSS"
     gene            complement(3795..4100)
                     /locus_tag="P5549_06770"
     CDS             complement(3795..4100)
                     /locus_tag="P5549_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12809.1"
                     /translation="MRSDLNAQLMIKCPGCKKSRNEYNWSMKTAARYSIGADTCPTLI
                     QVLLASLGGDEETFAGFRLICPKCNYGINYEDLEKPDAEEIRAYARAVGEEYCYFWY"
     gene            4266..4541
                     /locus_tag="P5549_06775"
     CDS             4266..4541
                     /locus_tag="P5549_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12810.1"
                     /translation="MIYCSACGKDANEYNWNLETAATFSEGDKTVPALLQLLLEALDD
                     PKKHADIQLVCPHCHEKVRLRQIPLPEREALLAYLQEVGEAYLHERF"
     gene            complement(4587..5738)
                     /locus_tag="P5549_06780"
     CDS             complement(4587..5738)
                     /locus_tag="P5549_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRW12811.1"
                     /translation="MKKKGWIGAGIAIAVALLIIQTLTGVQQVEVLKAETGDITTWVE
                     DTAYVQSRDEVTIQASQSGKIVRIAVETGEQVSAGQELLVMDNPDLQYQLNATAVQTE
                     QLQGQLSASVITVEQARLDLAEDQKNLGRARQLFESGAISQADYDASVQRNITLQKAV
                     ESQQQIQQSLRRQLEKQNTLYQELKGTSQQLVVKSTAAGMVLDLPIKEQQTVMAGASL
                     VQIGNINDLELKTELLSDDVGAVKVGQKATISAPLLGDKVLEGKVTKIYPRAYEKVSA
                     LGVIQRRVPVLISLSPSQLLEPGYEVQVRIETSLQQNVVTLPRTAIRVNEQGQNEVLA
                     IIDGKIKHSKVEIGIRNPEKAEVVSGLKAGQRVVRDASMDVKENTRVKI"
     gene            complement(5752..8130)
                     /locus_tag="P5549_06785"
     CDS             complement(5752..8130)
                     /locus_tag="P5549_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRW12812.1"
                     /translation="MSTLRRKLLRTIRTTRGQFIGLVFIVALGVMVYIGMGTAYGNLS
                     RSQQEFYQAANFADYYFQVVRAPSSVSSKIESIAGIVKASGRIQRDVSLVKEGSQRAV
                     GRITSYHLPMEGEVNQLHLLSGRMFASRSSGGGVEVLLDPQYADANHVQIDDHITIIA
                     EGRKVNLHVVGTAISPEFVYALPDAATMMPDPLNFGIIMMATEQAQQILDMPDQVNQV
                     VVKLAPGSDEKAIRQRTEELLRSYGNIASYPRKDQLSHAMLQAELDGLKINSHFLPLV
                     LLLIAAAIQFVILNRLVKSHRTQIGIMKAIGYNDYQIIGHYTTYALVVSVIGCIAGMI
                     AGVFLASSMSSVYSLYFNLPQAIGGINRQTLLQSVALSISVGAASGFLAARTVIRINP
                     AEAMRLPAPPQGNKILLERWAKLWTRLNSSWKMSLRSISRNRTRSLVTILGVMSAVVL
                     LVFSMFTNDAVDYMLNRNFNEVNRYDYTVRLAEPIEYREIQYWQQWKDIYQIEPQLEM
                     PIKIFYNGRSEDELCIGMTRDSDLKRVLNQAGERLQIPEEGLLISRRMAQRLGVRTGD
                     KVRVETKMSVGATHSTELLVTGINDQLMGSGSYVSLETANRMLDEKRIINMVMFKAAA
                     VSGDSIEKRFNAMPKVSSVTSRAKELQSFYDLMDTTVIFIGIMILLSALLGLVIVYNS
                     SLMAFNERRRELASLKVLGYSNPEIAGILRKETWLLAAIGIVFGLPAGKGMGQAYIAS
                     VSTDLYSLPVIIYPRSYLMAALGAVVFVAIGHYLAVRKAGQLDMVEVLKNRE"
     gene            complement(8130..8843)
                     /locus_tag="P5549_06790"
     CDS             complement(8130..8843)
                     /locus_tag="P5549_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12813.1"
                     /translation="MELEKELMRFEKITKIYDMGEVRVEALKESSFYVCRGETMVILG
                     PSGSGKSTLLNIMGGMDTPTSGQLFFAGRNLSQADSKELTRYRRRDIGFIFQFYNLIP
                     DLTAYENIALAAELVDEPLPVDEVLEEVGLTERRNHFPAQLSGGEQQRVAIARALVKR
                     PQLLLCDEPTGSLDDQTGKMVLGLLTRISGKENMTVVIVTHNTSIGQIADRTLRLRSG
                     QIVEMIVNENPLPAERIEW"
     gene            complement(8921..10633)
                     /locus_tag="P5549_06795"
     CDS             complement(8921..10633)
                     /locus_tag="P5549_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRW12814.1"
                     /translation="MATADNETNEIISSGNFIQTIITEDLQTGKNGGRVHTRFPPEPN
                     GYLHIGHAKSICLNFGLAASNGGICNLRFDDTNPSKEDVEYVDSIQADVKWLGFDWGD
                     RMFYASDYFEQLYQFAVQLIKASKAYVCDLSAPEIREYRGTLTEPGKNSPYRERPVEE
                     NLDLFIRMRNGEFEDGSRVLRAKIDMASPNLNMRDPVLYRILRATHHRTGDDWCIYPM
                     YDYAHPLSDAVEGITHSVCTLEFSDHRPLYDWCLDHVDYDAATHGRPQQIEFARLNLN
                     YTVMSKRKLRELVEKKYVNGWDDPRMPTISGLRRRGYTPPSIRDFCTRIGVAKRDSTV
                     DIALLEHCVREDLNLNAARVMAVLRPLKVIIDNYPPEQVEWLEAENNPENPDMGTRRI
                     PFSREIYIEQEDFMENPPKKYFRLTPGGEVRLKSAYIIKCEQIVKDMQGNIIELHCSY
                     DPDSKSGGATSGRKIKGTSHWVSATHAIPAEARLYEHLFLTENPDDDSDGSDYKSKLN
                     PDSLQVLEHCMVEPGLAQASSENRYQFLRQGYFCLDLDSRPDKLVFNRIVSLRDSWAK
                     AQKS"
     gene            11113..12945
                     /locus_tag="P5549_06800"
     CDS             11113..12945
                     /locus_tag="P5549_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12815.1"
                     /translation="MSYRFSFNTRDTKFIMKEWLPVEEILAYPKYSDYYSLDDFDTII
                     DQVRKISEDIIAPTAEDADAIGAQWRDGQVYIPESFHKAIRFLHDNGWATSNYDEEAE
                     GVLPMVLFHALAEIQAAANPALFAYTATGSGVVAVMNHYASDEVKAKFLPKLLDGTWG
                     GTMCLTEPTAGSDVGDMLSKAFPTDTPGLYKIKGQKIFITCGDRDDMENIIHLYLARV
                     EGAKAGTKGLSLFAVPKYWINEDGSLTPNDFQCIGIEHKMGMKGSATCALVAGENNNC
                     YGYLIGDPPNEEGVGQGMAQMFHMMNEARLDTGRLSLAVAANAYWAAKDYGKERIQGR
                     LMTDPKAGRTTINKHEDVKRMYMLNKATTEACRALLSKCYFYDDMRENDPDPEKRKWA
                     QGKMDMLTPLCKAYPSDEVWMLTAESLQSYGGYGYCEDYPAANSARDCKIWSIWEGTN
                     FIQSMDLIGRKWLQGKGAIFASVLGEIDEFIAKYEGNFPGMEKEIETLKKALAAYREI
                     QMTIGGFMGAGKVGLMPIYARRILTATAQLYCGYLLLDQAVICKKRMEELGPDHYDYN
                     FYYGKVLSTRYYLNNTVPNVWHVLEIIKMADTSILEAPVEIFDF"
     gene            complement(13001..13480)
                     /gene="rlmH"
                     /locus_tag="P5549_06805"
     CDS             complement(13001..13480)
                     /gene="rlmH"
                     /locus_tag="P5549_06805"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRW12816.1"
                     /translation="MRYRIISVGKIREKFYREGIKEYLKRLSPYGSFELLEGLEEKVS
                     SHPGDKEISRVLEKEGEGILRLIGENEILIALDLCGETFGSEQLADQLKHWHESGQSR
                     INFVIGASHGLAPKVIKRADQCISFSKMTFPHQMAVLILTEQVYRGFKILKGEPYHK"
     gene            complement(13585..15402)
                     /locus_tag="P5549_06810"
     CDS             complement(13585..15402)
                     /locus_tag="P5549_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW12817.1"
                     /translation="MNKEYDYKQNTVLDPFRDGVKRYGDRVMLRKRTDKGWVDLSWNQ
                     VKDQVDALASYMINMGLQKGEILSIYSQNRPEWVVADYATFSCAAVNASIYPTNSAPE
                     AAYILDDTKSVICFCEGKFQVDNVLAEKKNLPSLKKIIVFEDLDYKDDMVIKFTDALK
                     EGEKNLRHEEISARSASIKPSDLMTLIYSSGTTGQPKGVMLSHSNLMFISTTFKRIEM
                     LQEGDLLLSLLPLSHSVERSMSYYGVVLAGGIIAYSRGTEFFAEDLSEIRPTIGVFVP
                     RVFEKIYNGIFAKVATAPPGKQKIFARAVAVGKEAAPYFMANKKLPFILGLKYKVFDK
                     LVYANLKTTMGFERTRVFGSAGAPLLSEIHDFFWGLNMQIRKGYGLTETSPVLNVDGD
                     PSIMPIKSDGWITPFPGAEVKIAEDGEILARGPGVMLGYLNKPEQTNEMFTEDGWLKT
                     GDIGLLDRQGYVKIIDRKKDIIVTSGGKNVAPQVLENTFASHSFIEQIAVLGDGKKYI
                     VALIVPSFETLKEWAAKEGLNGLGNEQLVSDEKVIRKYEEIINELNQHFGRVEQVKKF
                     KLMPQELTEESGELTPTLKLKRKVIMSKYQQEIESLYVE"
     gene            complement(15587..>16488)
                     /gene="murA"
                     /locus_tag="P5549_06815"
     CDS             complement(15587..>16488)
                     /gene="murA"
                     /locus_tag="P5549_06815"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRW12818.1"
                     /translation="GCDIGSRPMDLHLKGAQALGADIEIEHGYIYARTKDSFGSRVYL
                     DFPSVGATENIMMMASKTPGQTIIENSAKEPEIVDLANFLNSMGAKIRGAGTDIIKIE
                     GVPVLKAVHYAIIPDRIEAGTYMIAAAITGGEIQVENIIPTHLHPIIAKLQETGAVIR
                     ETEQGLLVKAGERILPADIKTMPYPGFPTDMQSQMIALLSLAEGTSVVVENVFENRFQ
                     IIDEFIRMGAKIKLEGRSAIVEGTPHLYGARVKATDLRAGAALVLSGLAASGTTIIDD
                     AIHIFRGYENIEEKLMSLGAKIK"
BASE COUNT         4103 a         4373 c         3709 g         4303 t
ORIGIN      
        1 cggaagggtc accgatatag gcagttccgc cgccgatcag taccaccggt ttgttacctg
       61 ccatttgcaa gcgtttcatc aggcatagcg ccatgaaatg acccacgtgc agagaatccg
      121 cggtgggatc aaaaccaata taaaaaatag cctggccgct attaacaagt tctttgattt
      181 gttcttcgtc tgtaacctgg gcaattaaac cccgggctac cagctcgtca taaattttca
      241 ttactacact cctttgcgga cttttgccat gtacagggat tgattcttaa agcatcctga
      301 cgggatttag cttttctttt tagactttat ataacctcgt gcgtttatta tataatatgc
      361 ttgatgctgc cggcaaaaaa actggcgccg aaaccgacca gacacaaggc cagaaccacg
      421 atgatggtct tgtatatggc aatgttgagc agatgccggc tcttgctgac catcgccgcc
      481 acaaaagtat accaggtaat atcccccagg atatggccca gataaaagat gacgatcccc
      541 catacaccca gggagtggta tgcactcata atcaaagcca gtcccaccgc cgaccaccaa
      601 atcagaaaat aaggattggt ggcacttaaa accatcccgg ccataaacat gccgccctgc
      661 ttgtgatcat gcatttcacc cgtttccaga gtgattttat tcaaatatac atctttgagc
      721 atctgggagc caagataaac cagtaccacc cctccgatca gcccgatggc aatgccggct
      781 gattctgctg ccagatagcg gccggcgccg acaaaaatca ggatcaccag gatcaactcc
      841 agcagggcat gtcccagcga taccagcacg ccagccttga ccccgtggcg caggctccga
      901 tcaacggtat aggtaaaaac gctgcccggc atgacggcgc cggaaaaagc gattagcaaa
      961 gacttaaaaa atatagccag catgacgatt cctcatttag cgagtctttt tgatcccctc
     1021 cggatgatgc aggacgcgct tgcggctctg gcttttggtt ttgctgataa aaagcaaacc
     1081 cacgatcagg gttgcccctg cgccccagcg cgattcgact gtaaatccgt tgacatagag
     1141 acagatcgtg ctcaacatga ctgcggacag aacaattccc caggcaatgg tcagactggc
     1201 acgggtattc tgctccaggt tttcctgcag gtttttgagc ggcagtttga gctccagatc
     1261 gcctctttca gcctgccgca ggacttttgc caacaacggc ggtccttcga tcagagcact
     1321 gccccaggca gccgctttgt ctttagctat tttcaaaggc cgggcttcct ctggcataaa
     1381 ttgtttcaga tacggcttgg ccgcatccag aaaactgata ttcttgtcca gtccgataca
     1441 aatcccgtaa agggtgccca gcgcccgtcc caaaaatgta aagcgggccg ggatctgaaa
     1501 aggctgttca tagagaattt cctccagatc atccaaaagg ctttttagat cattgtcgaa
     1561 caagtcggtt ccctgcccca gcagctgttc cagaaatacg cctaccgccc gggcaatcag
     1621 tgcatcgtcg gcatcccggc gcaaaaatcc cacctgcttc agataataaa ccacctgcag
     1681 attgtctcga tcaaccatgg ccaaaaccat tttaatcagc ttttctttta gttccgggga
     1741 gatggtcccc accatcccga aatcgatcat aatcactttg ccgtccggat cgatgaaaag
     1801 gttgccggga tggggatcag catgaaaaaa tccatccacc aggatctgtt ttatataaat
     1861 ttccaacagg cgactggcca gttgggaacg gtcaatcccg gcctgttgca gacctgcata
     1921 atcggttacc ttgtaaccat cgacaaactc catggtcagt acccgttggc gggtgtattc
     1981 ccaataaatt cgggggacga ttagttcctt gtcggccaca ctatgagctg caatcgtctc
     2041 ggcatttttt ccttcctgca ggtagtttag ctcttcccgc aaggtttctt caaattcacg
     2101 gtaaatggcg tcaaaatcca cccactgctc ccaatcggta aaaaattgaa tccagccgat
     2161 cacctgacgc accgcccgca ggtcgatatc aatcagtttt tcaatattgg gccgcaagac
     2221 ctttacagca gccttctgcc caccgaacag ctctgcctca tgaacctgcc ccaaagaagc
     2281 ggaagctagg ggagccggat tcaagactgg ataaatctca tgcaggggcc tgccaaattc
     2341 actttccaga accatgcaca tctctttaaa ttcgaccgcc ggcacctcat cttgcagatt
     2401 ggccagctcg cgggtgacgc tctgcgggaa aatatccacc cgggtgctga agaactgccc
     2461 cagtttaatc agcaggcccc ccatttccac ggcggtattg cgaaacagac gggcctctgt
     2521 gacatacagc tctatccgcc gctgctcttt ccaaccgtcg gcatgccata ccgctttgat
     2581 ctttaaagaa taaaaagacc agaaaatatg caaaaaaaga cggatgaccc gaaaaaatct
     2641 ctttattcct gcccaggaac gctgtctcat aaattgtcct ccacctcaaa ttgctgctga
     2701 tggttggtac tatgcaccat catctcgctc attataccat gtaatgcggg gctgtaaaaa
     2761 cgctgcagcc ggggataaaa aaatattccc gcgttttttg cgattagcaa ggaaattcat
     2821 gcccagtaaa gaatagtaaa gatattcggc agatgtgaag ataatacacc ggtcgctgtt
     2881 cctgccgacc agaacctgga tagaaggtga aacaaatagc aacagaacaa ttcactttac
     2941 atataatccc gggatatatt gaaaatcttt ttctggctga ataccaacat ggattgctgc
     3001 tgctggactg cggcgcggtc agtgatgtaa agcgtatcga ggatttctgc cgccgggatt
     3061 tgcatcgttc cccgaaggat atcaggctgg cggtcgtgac tcatatgcat ccggatcacg
     3121 ccggaggggc ggtgctcttg cgggataagt tcgggattcc cctggccggt catcaagaac
     3181 tggatcgctg gtatgccggt cctgggggtt ggatgcaaaa agtgatcgac tgtctgctga
     3241 tgcagtcggt ggcgtggcgg acgcgccagc aacttgaaaa aggttttttc tgcagccgta
     3301 tacaacctga tttcctgttg aaggacggtg atgcactgcc tttcttcccg gactggcaag
     3361 cagtggcagt gccaggtcac acctctcatg atctggtgct gtatcatgcc gatcagtccc
     3421 ttttgtatgt agccgactgc tttttggaag tcaaaggccg ctatcagaca ccgctgccgg
     3481 tcatgtttcc ccaatggctg gaggcttcct atcaaaaaat gggcgccctg catccatcca
     3541 gcctcttgat tgcccacggc gaagcccgcc gtgatcaaat cccggcggac ttgttcagct
     3601 ccctgcagca tctgctgcag actcccccca gcaaattgac ccggcggatc aaaaagctgt
     3661 ccgtctatac tcctgaatat catcgacaaa acaaaatctt ccaacaaacc tggcgcaaaa
     3721 atagaaggag aagaaactcc agctaagccg attcgatgga aagaaaagtg caccgtcggt
     3781 ctccgccagg cagtttaata ccagaaataa cagtactctt ctcccaccgc ccgggcataa
     3841 gcgcggattt cttctgcatc cggcttttcc aaatcctcat aattaatccc ataattgcat
     3901 ttgggacaaa tgagacgaaa accggcaaag gtttcctcat ctccgcccag cgaagccagc
     3961 aacacctgaa tcaaagtcgg gcaggtgtcc gccccaatgg aataacgggc ggcggttttc
     4021 atgctccaat tgtattcatt gcggcttttc ttgcaaccgg gacattttat catcaactgt
     4081 gcatttaaat cgctgcgcat gattgttctc ctcattttcc ggcaaactta tgtacccgac
     4141 atgattataa cccgccaggc ttgttttttg tgcgcgcctg acatagaata atgtcgttac
     4201 acagttcacc aacgattctt attggggagg gaaagaccat ccgtaaaaat ccagccgcca
     4261 ccctgatgat ttactgttcc gcttgtggca aggacgccaa tgaatataac tggaacctgg
     4321 agaccgcggc caccttttcc gagggtgata aaactgtgcc tgcgttgctg cagctgctcc
     4381 tggaagcatt ggacgatcct aaaaaacatg ccgatattca actggtctgc ccccattgtc
     4441 atgaaaaggt acgcttgcgg caaatcccgc tgccggaacg cgaagcgctg ctggcctatc
     4501 tgcaggaagt cggcgaggcg tatctgcacg aacgttttta atcttgttgg cccccattgt
     4561 aaatgtcttc ctcgtcaaca agactttcat attttaacgc gggtattttc ttttacatcc
     4621 atactggcat cccgtaccac cctctgccct gctttcaaac cgctgacgac ttcagctttt
     4681 tcaggattcc tgatcccaat ctccacttta ctatgtttga tctttccatc gataatcgcc
     4741 agcacttcgt tttgcccttg ttcgttgacc cggatggcgg tacgaggcaa cgtgaccaca
     4801 ttttgttgca agctggtctc gatcctgact tgcacctcat aacccggttc caacagttgt
     4861 gacggtgaca atgagataag caccggtaca cggcgctgaa taacccccag ggcggaaact
     4921 ttttcatagg cacgcggata gatcttggtc actttccctt ccagcacttt atcacccagg
     4981 agcggagcac tgatcgttgc cttttggccg accttgaccg cgcctacgtc atcgctaagc
     5041 agttctgttt tcagctccag gtcgtttata ttgccgattt gtaccagaga tgcccccgcc
     5101 attacggtct gctgctcttt gatcggcaga tcgagcacca tcccagcagc ggtacttttt
     5161 accaccaatt gctgactggt ccccttcaat tcctggtaca gggtgttttg tttttccaac
     5221 tgccggcgca atgactgctg gatctgctgc tggctttcaa ctgccttctg cagggttata
     5281 tttctttgca cactggcatc ataatcagcc tgagaaatcg ccccggactc aaaaagctgc
     5341 cgggcgcggc ccagattctt ctggtcttcc gcaagatcca gccgggcttg ctccaccgta
     5401 atgacggatg cggacagttg tccctgcagt tgttccgtct gtaccgcggt ggcattcagc
     5461 tgatactgca gatcgggatt atccattacc agcagttcct gcccggcact tacctgctcg
     5521 ccggtttcaa ccgctattct gacaatcttg ccgctctggg aggcctggat ggttacttcg
     5581 tcccgggatt gcacataggc ggtatcctcg acccaggtag ttatgtcgcc ggtttccgct
     5641 tttaatactt ccacctgctg cacgccggtc aatgtctgta tgatgagcaa agcgactgct
     5701 atggcaatac cagctccgat ccaacccttt ttcttcattt ccttcctccc tttactcacg
     5761 atttttcaga acctcaacca tatccagctg acctgccttg cgtacggcca gataatgacc
     5821 aatggccaca aacacgaccg cccccagggc agccataaga taactgcgcg gataaataat
     5881 caccggcaga ctgtaaagat cggtgctgac gctggcaata taagcctgac ccatcccctt
     5941 gccggccggc aggccgaaaa ctataccaat ggccgccagc agccaggttt cctttcttaa
     6001 aataccagct atttcagggt tcgaatagcc cagcactttc aaggaagcca gttcccttct
     6061 acgctcatta aacgccatta gggatgaatt gtaaacaatg accaatccca gcaaagctga
     6121 cagcaaaatc atgatcccaa taaaaatgac cgttgtatcc atcaggtcat aaaaactctg
     6181 cagttctttg gcgcggctgg tcacggaact taccttgggc attgcattaa atcgcttttc
     6241 aattgaatca ccactaacgg cagcagcttt aaacatcacc atattgatga tccgtttctc
     6301 gtccagcatg cggttggccg tttccagtga aacgtaggag cccgatccca ttagctgatc
     6361 attaatacct gtgaccagca actccgtgct gtgcgttgca cccactgaca ttttggtttc
     6421 caccctgact ttgtcaccgg ttcttacgcc aagtctttgc gccatgcgcc gactgatcag
     6481 caatccttct tccggaatct gcaaccgctc gcccgcctgg ttcaaaaccc ttttcagatc
     6541 actgtcacgt gtcatgccaa tacaaagttc atcctcactg cggccgttat aaaagatttt
     6601 tattggcatt tcaagctgcg gctcaatttg ataaatatct ttccattgct gccagtattg
     6661 tatttcccta tattcaatcg gttcggccaa ccgcacggta taatcataac ggtttacctc
     6721 attaaagttc cggttcagca tataatccac cgcgtcgttg gtaaacatgg aaaataccag
     6781 cagcaccacc gcactcatta cccccaggat ggtaaccagg gaacgggtcc ggttgcggct
     6841 gatggatcgc aggctcatct tccagctgct gttcagcctt gtccataatt tagcccacct
     6901 ttccagcagg atcttgtttc cctggggcgg ggccggcagt cgcatagctt cagcagggtt
     6961 tatcctgatc accgtacggg cagccagaaa tcctgaagca gcaccgaccg atatgctcaa
     7021 agcaacgctt tgcagcaacg tttgccgatt aattcctccg attgcctgtg gcaaattgaa
     7081 atacagactg taaaccgagg acatggatga ggccaaaaat acccccgcaa tcattcccgc
     7141 aatgcatcct atcacactta caacaagggc ataggtagta taatgaccga tgatttgata
     7201 atcattatag ccgatggctt tcatgatccc aatttgggta cggtgtgatt taaccaaacg
     7261 atttaaaata acaaactgga tcgccgctgc gatcaacagc agaaccagcg gcaaaaaatg
     7321 agaatttatc tttagtccgt ccagttccgc ctgcagcatg gcgtgactca gctgatcctt
     7381 gcgcggatag ctggcaatat taccgtagct tcgcaggagt tcctccgtcc tttgcctgat
     7441 ggccttttca tccgatccgg gcgccagttt gaccaccacc tgattgacct gatcaggcat
     7501 atccaggatc tgttgcgctt gttcagtagc catcataata atgccaaaat tcaggggatc
     7561 gggcatcatg gttgcagcat ccggcaaggc gtagacaaat tcagggctaa tggcagtacc
     7621 cactacatgc aggttgactt tcctgccttc ggcaatgatc gttatgtggt catcgatttg
     7681 gacgtgattg gcatcagcat actgcggatc caacaagacc tcgacacccc cgccggagga
     7741 gcgggaagca aacatccggc cgctgagcag atgcagctga ttgacttccc cctccatggg
     7801 cagatgataa gatgtgattc ttcccaccgc ccgctggctg ccttccttaa ccaaggaaac
     7861 gtccctttga atccgtccgc tggccttgac tatccccgct attgattcga ttttactgct
     7921 gacagaagag ggtgcgcgga caacctgaaa gtagtaatca gcaaagttgg ccgcttgata
     7981 aaattcctgc tgggatcgac tgaggttgcc gtaagctgtc cccatgccga tataaaccat
     8041 tacccccagg gcaacgataa ataccagacc aatgaattgg ccgcgggtcg tgcggatggt
     8101 tcgcaatagc ttgcggcgca gggtcgacat taccactcaa tcctttccgc cggcagagga
     8161 ttttcattga caatcatttc aacgatttgc ccgctgcgca ggcgcagcgt gcggtcagca
     8221 atctgtccga tactggtatt atgggtaacg atgacgacgg tcatattttc cttgccgctg
     8281 atccgggtca gcaatcccag caccattttg ccggtctgat catccagcga gccggtcggt
     8341 tcatcacaca aaagtaattg tggccttttg accagcgccc gggcaatcgc cacccgctgc
     8401 tgttcgcctc cgcttaactg ggccggaaaa tgattgcgtc gttccgtcag cccaacttcc
     8461 tccagtactt cgtctaccgg caagggttca tccaccagtt cggcggccaa ggcaatattt
     8521 tcgtaagcag tcagatcagg gataagatta tagaactgaa aaataaagcc tatgtcacga
     8581 cggcggtatc gggtcagttc tttactgtca gcctggctca ggttgcggcc ggcaaaaaac
     8641 aattgtccgc tggtcggcgt atccatgcct cccataatat taagcaaggt gcttttgccg
     8701 gagccgcttg gccctagtat taccatggtt tctccccggc aaacataaaa tgagctctcc
     8761 ttgagggctt ccaccctcac ttcacccata tcatatatct tggttatttt ttcaaagcgc
     8821 atcagctctt tttccagctc catggcttca tctccctttg cataaaatct cctgcttaat
     8881 gaaagcgcga accggaccgg ttcgctctta ttaacattgg ttatgatttt tgtgctttgg
     8941 cccagctgtc acgcagcgat acgatgcgat taaacaccag cttgtcggga cgcgagtcaa
     9001 ggtcaagaca aaagtagccc tgacgcagga attggtaacg attttcgctg gaagcctgtg
     9061 ccaatccagg ttcaaccata cagtgctcca ggacctgcag cgaatccggg ttgagcttgc
     9121 tcttgtagtc gctgccatcg ctgtcatcat ccggattttc ggtcaaaaaa agatgctcat
     9181 agaggcgcgc ctcagccggg atggcatgcg tggctgacac ccaatgggaa gttcctttta
     9241 tttttcggcc gctggtcgct ccgccgcttt tggaatccgg atcataactg cagtgcagtt
     9301 caataatatt gccctgcatg tccttgacga tctgttcaca tttgataata taggcgctct
     9361 tgagtcttac ttctccccct ggcgtcagcc gaaaatattt tttcggcgga ttttccataa
     9421 aatcttcctg ttcaatataa atttcccggg agaaaggaat ccggcgggta cccatatcag
     9481 gattttccgg attgttttcc gcttccagcc attccacttg ttccggcgga tagttgtcga
     9541 tgatgacttt taaaggacgc agcacagcca taacccgagc agcattgaga ttcaagtctt
     9601 cccgtacaca gtgctccagc aaggcaatgt ccaccgtgct gtcacgcttg gccacgccaa
     9661 tccgggtgca gaagtcgcga attgatgggg gggtgtagcc ccggcggcgc agacctgaaa
     9721 tagtgggcat acgcgggtcg tcccagccgt tcacatattt cttttccacc agttcacgca
     9781 atttacgttt gctcatgacc gtataattca ggtttaagcg ggcaaactca atttgttggg
     9841 gtcttccgtg ggtggcggca tcataatcga catgatccag gcaccagtca tagagcggac
     9901 gatgatcgga aaactccagg gtgcagacgg aatgagtaat tccttccaca gcatccgaca
     9961 agggatgcgc ataatcgtac atgggataga tgcaccaatc atctccggta cggtggtggg
    10021 tggctctcaa tattcgataa agcaccgggt cacgcatatt cagattggga gaagccatgt
    10081 cgattttggc ccgcagaacc cgggagccgt cttcaaattc gccattgcgc atgcggataa
    10141 acagatccag gttttcttca acgggacgtt cccgataggg actgtttttt cccggttcag
    10201 taagcgtccc gcggtattct ctaatctccg gggcgctcag atcacataca taggctttgc
    10261 tggccttgat caactgcacg gcaaattgat acaactgttc aaagtaatcg gaggcgtaaa
    10321 acatacgatc gccccaatca aaacccagcc atttgacatc agcttggatc gaatcaacgt
    10381 attcgacatc ttccttgctg ggattggtat catcaaagcg caggttgcag atcccgccgt
    10441 tactggcagc caagccaaag ttcagacaga ttgacttggc atgaccaata tgaagatagc
    10501 cgttgggttc ggggggaaag cgcgtatgta ccctgcctcc gttcttgccg gtttgcagat
    10561 cttccgtaat gatggtttga ataaaattgc ccgatgatat gatttcattg gtttcgttgt
    10621 ccgctgttgc cattgcatat cctccttata ttcaaaggga cttttctgtc ctttgtgtac
    10681 aacccaggcc ggttcattcg gcccaaacag aataccataa ttttaatcat tatacctttt
    10741 tgcatattaa ttctgtagta ggttcaagta attaatttac agcaattaaa ccccataaat
    10801 aataagtttt ctgtcgttaa aagattcttt tgctagtatg gagtctttca ttttatttaa
    10861 tgcaaaatta aaaatattga ctttattgac tttaataata cctttgtaat atgaaaaatg
    10921 tctaatattt aaagcaaaag tactaaaaat gaatacgcca ggcaggatat tcttaatctt
    10981 cagcgaattg tattatgata gaaatgatat tcatttgctt gataatctta aatctggaaa
    11041 ggaggccgtt ataccgaaat actccgtgta ataaatttct acctgaaaaa atctggaaag
    11101 gaagcgtgag aaatgtccta taggttttca ttcaacacca gagataccaa attcataatg
    11161 aaggaatggc tgccggttga agaaattctg gcttatccca aatacagcga ttattatagc
    11221 ctggatgact tcgacaccat cattgatcag gtccgcaaaa tttctgaaga cataatcgct
    11281 cccactgccg aagatgcgga tgccatcggg gcacaatgga gagacggcca ggtctatatt
    11341 cctgagtcat tccacaaagc gattcgtttc ctgcatgaca atggctgggc caccagcaac
    11401 tatgacgaag aagccgaagg cgtacttccc atggtgctgt tccacgccct ggcagaaatt
    11461 caggccgctg ccaaccctgc cctgtttgcc tataccgcaa ccggaagcgg cgtagtggcc
    11521 gtaatgaatc attatgccag cgacgaagtc aaggcaaaat tcctgcccaa acttttggat
    11581 ggtacctggg gcggaaccat gtgtctgacc gagcctactg ccggatctga tgtcggcgat
    11641 atgctatcca aagcattccc caccgatacc cccggccttt ataaaatcaa agggcaaaaa
    11701 atcttcatca cttgcggcga ccgtgatgat atggaaaata ttatccatct ctatctggcc
    11761 agagtcgaag gcgccaaagc cggcaccaaa ggattatccc tttttgccgt tccgaaatat
    11821 tggatcaatg aagacggcag cctgaccccc aacgatttcc agtgcattgg cattgagcac
    11881 aaaatgggta tgaagggttc ggccacctgc gcattggttg cgggcgagaa caacaactgc
    11941 tacggctatc tgatcggcga tccgcccaac gaagaaggcg taggtcaggg tatggcccaa
    12001 atgttccata tgatgaacga agctcgtctg gataccggcc gtctgtccct ggcagttgcc
    12061 gccaatgcct actgggctgc caaagactat ggcaaggaaa gaattcaggg ccgtctgatg
    12121 accgatccca aagccggccg caccaccatc aacaaacacg aagacgtaaa acgcatgtac
    12181 atgctcaaca aagccaccac cgaagcctgc cgggcactgc tgtccaaatg ctatttctat
    12241 gatgatatgc gcgaaaacga tcccgatccg gaaaaacgca aatgggctca gggcaaaatg
    12301 gatatgttaa ccccgctctg caaagcttat ccttctgatg aagtctggat gctgaccgca
    12361 gaatcactgc aatcctatgg tggatatgga tactgcgaag attatccggc tgccaactcc
    12421 gcccgtgact gcaaaatctg gtccatctgg gaaggcacca acttcattca atccatggat
    12481 ctgatcggcc gcaaatggct gcagggcaaa ggcgctatct ttgcatccgt gctgggtgaa
    12541 atcgatgaat ttattgccaa atacgagggc aacttccccg gtatggaaaa agaaattgaa
    12601 accctgaaaa aagcgctggc cgcttatcgt gagatccaga tgaccatcgg cggattcatg
    12661 ggcgccggca aagttggttt gatgccgatt tatgcccgcc gcattctgac cgctaccgct
    12721 cagctgtatt gcggttatct gcttctggac caggccgtca tctgtaaaaa gagaatggaa
    12781 gaactgggtc ccgatcatta tgactacaac ttctactatg gcaaagttct gtccacccgg
    12841 tactatctga acaacaccgt accaaatgta tggcatgtcc tggagatcat caaaatggct
    12901 gacacttcca ttctggaagc cccagtcgaa atctttgatt tctaaaaccc cagcaacgcg
    12961 aagccccgga gcatatcaat gctccggggc tttaatgtaa ttacttatga tacggttcac
    13021 ctttaaggat tttaaaaccc cgataaactt gttcggttag gattaatacc gccatttgat
    13081 gcggaaaggt catctttgaa aaggatatgc actgatcggc ccgctttatt actttcggag
    13141 ccaagccatg ggaggccccg attacaaaat tgatcctgga ctgtccactt tcatgccaat
    13201 gctttaattg atccgcaagc tgttccgaac caaaggtttc tccgcacagg tcaagggcaa
    13261 ttaatatctc attttccccg atcagacgca aaatcccttc gccttctttt tccaaaaccc
    13321 ggctgatctc tttgtccccg ggatgagaag aaactttctc ctccagtcct tccaacagtt
    13381 cgaaactgcc gtagggactc agccttttca gatactcctt gattccctcg cgatagaatt
    13441 tttctcttat ttttcctacc gagatgatgc ggtatctcaa tatatctcct cgctgttcga
    13501 tttacatttc acttattaac aaatattgta gcataaaaca atcgggcctc cgtcagacgt
    13561 aagcccgatt gcttaaaccc gaaattactc aacgtacaga gattcaatct cctgctgata
    13621 tttgctcatg atcactttac gttttagttt aagggtaggc gtgagttcac cgctttcctc
    13681 ggtcaattct tggggcatga gtttgaattt ctttacctgt tccacccgcc cgaaatgttg
    13741 gttcagttca ttgataatct cttcatattt ccgaatcact ttttcatcac ttaccagttg
    13801 ctcgttgccc agtccgttca gcccttcttt cgctgcccat tcttttaagg tttcgaagct
    13861 gggaacaatc agggcgacaa tatatttttt cccgtccccc agcacggcaa tctgttcaat
    13921 aaaggaatgg gaggcgaacg tattctccag aacctgaggg gctacatttt ttccgccgga
    13981 agtaacgatg atgtctttct tgcgatctat tatctttaca tatccctgac ggtccagcag
    14041 tccgatatct ccggttttca gccagccgtc ctcggtgaac atttcattgg tctgctccgg
    14101 tttgttcaga tagccgagca tgacccccgg tcctctggcc aggatctcgc cgtcttcggc
    14161 gatttttacc tctgcgccgg ggaagggcgt aatccaaccg tctgatttga ttggcatgat
    14221 ggacggatcg ccatcgacat tgagcaccgg cgatgtttca gtaagaccgt atccctttct
    14281 aatctgcatg ttaagtcccc agaagaagtc atgtatttct gacagcaacg gcgcgcccgc
    14341 acttccaaat acccgggttc gctcaaaacc catggttgtt ttcagattgg cataaaccag
    14401 cttgtcaaag actttgtatt tcagtcccaa gataaagggc agctttttat tggccataaa
    14461 atagggagcc gcttctttcc ccacagcaac agcccgggca aatattttct gttttccggg
    14521 aggagctgta gctaccttgg caaatatacc gttgtatatt ttttcaaata cacgtgggac
    14581 aaagaccccg atcgtaggtc tgatctcaga caaatcctcg gcgaagaatt ccgttcccct
    14641 cgaataggcg ataattccgc cagccagtac cacgccataa tagctcattg acctttccac
    14701 ggaatgagac agcggcagca gcgataacag caggtctccc tcttgcagca tttcaattcg
    14761 tttaaaagtt gtgctgataa acatcaggtt ggaatggctc agcataacgc ctttgggctg
    14821 tccggtagta cccgaggagt agatcagggt cattaaatcg cttggtttga tggatgcaga
    14881 cctggccgaa atctcttcat gcctgagatt cttttcccct tctttgaggg catcggtgaa
    14941 tttgataacc atatcgtctt tataatcaag gtcttcaaat acaataattt ttttcagtga
    15001 agggaggttt tttttctccg ccagcacatt atcaacctga aacttacctt cgcaaaagca
    15061 aatgactgat ttggtatcat caaggatata ggcggcttcg ggagcagaat tggtcggata
    15121 gatcgacgcg ttaacggcgg cacaggagaa ggttgcataa tctgccacaa cccattccgg
    15181 cctgttttgg gaataaatag agaggatttc ccctttttgc aaacccatat taatcatgta
    15241 tgatgccagc gcatctacct ggtccttgac ctgattccag gataaatcaa cccagccttt
    15301 gtctgttctt tttctgagca taacccggtc accgtatctt tttacaccgt cacggaaagg
    15361 atccaacact gtattttgct tgtaatcgta ttctttgttc atccgcttga cctccttatt
    15421 tttttgttac tgccaatctt tttcctttcc tggttgcgct agcatattta ttagtttttt
    15481 taatatttcc cgttgatttg tcataatcct gcaagaaaag actgattctt taacattatt
    15541 gaaaaagaat cagcctcctg acatgaacac agtttagtcg ctctttttat ttgattttgg
    15601 cacctagtga cattagcttc tcttcgatat tttcataccc gcggaagata tggatggcgt
    15661 cgtcaataat ggttgtcccg gaagcagcca gaccactcag aacgagggct gccccggctc
    15721 taaggtcagt ggctttgacc cgggccccgt ataaatgtgg tgtgccttcc acgatggcag
    15781 atcggccttc gagtttgatt ttggcgccca tacggataaa ttcatcaata atctggaaac
    15841 gattttcaaa tacgttttcc accaccacac tggttccttc cgccaatgac aacagcgcaa
    15901 tcatctgcga ctgcatgtcg gtgggaaatc cgggataagg catggtcttt atatccgccg
    15961 gcagaattcg ttctccggcc ttgaccagca gaccctgctc ggtttccctg attaccgctc
    16021 cggtttcctg aagcttggcg ataatgggat gaagatgggt gggaataata ttttccacct
    16081 ggatctcccc accagtgatg gcagccgcga tcatataagt accagcctcg atccggtcgg
    16141 gaatgatcgc gtaatggacg gccttcagga ctggtacgcc ttcaatcttg atgatatctg
    16201 taccggcacc tcttattttg gcgcccatgg agtttaaaaa attggccaaa tcaacgattt
    16261 ccggctcctt ggcagaattt tcgataatgg tctgtccggg ggtcttgctg gccatcatca
    16321 ttatattctc ggtcgctcct acgctgggaa aatccagata tactctcgag ccaaaagaat
    16381 ctttggtacg agcataaata tagccgtgct cgatttcaat atctgcacca agagcctgcg
    16441 cccctttgag atgcagatcc atcggtcggg aaccgatatc acagccgc
//
LOCUS       DAOTHZ010000048        16422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4265_length_16422_cov_8.459767, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000048 DAOTHZ010000000
VERSION     DAOTHZ010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 16422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16422
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4265_length_16422_cov_8.459767"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..901)
                     /locus_tag="P5549_06820"
     CDS             complement(113..901)
                     /locus_tag="P5549_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12819.1"
                     /translation="MQERINIWSSVVVARLPEKKICQHTRECRTAVGFRRIEEMKTRI
                     SARQVTCKGRAAELKVNVETLFVMEDEQGNLLPFSRRDIITESVALTEFLPQLEAGQE
                     LVYVAEIKAFNGDSILRDQNLIITYDMTFMLLALREQIVSLQPERIGQANLTGLNPAD
                     ENLFNASQRENIDLRRQIRIYEMNLLSLKKSLQKAETVNAELQRQIRLLRQAEEAFVI
                     PRNMDESKQRKSKNDEGKIISFYTREERRKQMGKKIKEFFINNA"
     gene            complement(1088..1366)
                     /locus_tag="P5549_06825"
     CDS             complement(1088..1366)
                     /locus_tag="P5549_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HRW12820.1"
                     /translation="MNSPRAIAEIKKDLESFVGSRIRLKANRGRNRIVEKEGVLESIY
                     PNIFVVKLNERKIDRRVSYSYADVLTETVELFVYSAKNEEIKIASANN"
     gene            complement(1489..2349)
                     /gene="rsmA"
                     /locus_tag="P5549_06830"
     CDS             complement(1489..2349)
                     /gene="rsmA"
                     /locus_tag="P5549_06830"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRW12821.1"
                     /translation="MVVLASAKGVQEIMQRFNIHARKGLGQNFLVDQNILTRIADAAE
                     LDKSQYIVEIGPGLGALSRELARRGRGLLTIDIDQRLEAVLQETMAGFDNVRLLFNDI
                     LKTDIEAELQKQFELNEMVTYKVCANIPYNITTPIIFKLLESCPHLQSATLMMQKEVG
                     ERILAKPGSKDYGRLTVAAAYHAKVEKVIQVSRNCFYPKPEVDSIVLKFIPHSKKILA
                     PDEEIVLQQILNGFFQQRRKTILNLIVNIFQIDKNIAAEKLQMIALAPALRPENLDFY
                     DYIKLVKAFY"
     gene            complement(2495..3049)
                     /locus_tag="P5549_06835"
     CDS             complement(2495..3049)
                     /locus_tag="P5549_06835"
                     /inference="COORDINATES: protein motif:HMM:NF021120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HRW12822.1"
                     /translation="MSQAKLLGFVLMALILLTSGCQQAQKPYSTPNANPNATPAHPIQ
                     VTESDKRIMANRFSELAKTVPGVVNATVVVAGADAAAKGTSTNATKEISTSSPGKIVV
                     MVGITLNTEVMASATQQASTKKMVKQKILDSDKRVTDVLVTSDANMVKKINDVAAGLI
                     EGKPVLSYARSAAELGKMMKEQVK"
     gene            complement(3209..4177)
                     /locus_tag="P5549_06840"
     CDS             complement(3209..4177)
                     /locus_tag="P5549_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRW12823.1"
                     /translation="MDLAWSKKGTILIASLLTLGILVTSMFFALQKPVTIAADGKLIQ
                     TRVLFSGTVKDVLQKNHIGLGKNDLVQPILDSKVTRHTQIVVTRAFKVRVLADGTIKT
                     IETTPVTAEKAVSLAGFQLGENDIIKTKAGKLVSTNQEIEVIRVTEQIVNEDQTIPYS
                     SEQIDDPTLERGLTKTVKAGKEGIARNTIKIIFYNGQEEKREIISTETLQEPQNRVVA
                     MGSITSVSRGSERLDFREAKYMQASAYTYTGFRTATGLNPSVGMVAVDPNVIPMGSRL
                     YIEGYGYARAADTGGAIKGNTVDLFMESYSQCINWGRRSVKVYVLN"
     gene            4585..5925
                     /locus_tag="P5549_06845"
     CDS             4585..5925
                     /locus_tag="P5549_06845"
                     /inference="COORDINATES: protein motif:HMM:NF027437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4829 domain-containing protein"
                     /protein_id="HRW12824.1"
                     /translation="MLNDRKSSFWVVVFAVVAVVMVGIAFFTTPPREDTVQQQDIKSN
                     PPSVAPEGGFTTRYETVKIEFLTDMKGFKSTDRFTSTDLEIVAYIDATLTNSQTAVQA
                     NDMNNNHTNQYLIELSNVIGGYSCGLYYDTLYDKAYLVKDGGLYATGTDFARYIDALL
                     ENTKIIAHIDDTAAVKLFQAYGWTLDYKINTRPDKLNDIRVLKEFQPNAYYFAYNNEL
                     SKEIGLDMSSYSNSPNINVDIYRIHESMPQEFYPIQNCRGIVIKKGNKIIGAFISAGR
                     HSAFNACSLKANRFEKATGQTLDQWLARMIKADRIEERLSKLEPEEMIEEYFIALDQK
                     NTIAAAYCISKKTLLENLTSNMLNEQLYNENISLPLTDAGIEAKSSFTNLESANLLHI
                     EPMDSSDKNTKIFRVTVDLQYHQALTIGNGQQLWDCSMVFESPQTGWKIAGFGH"
     gene            complement(5951..6721)
                     /locus_tag="P5549_06850"
     CDS             complement(5951..6721)
                     /locus_tag="P5549_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRW12825.1"
                     /translation="MLIDTHAHLQDEDLKKELDQVLNRAQAAGVEKIICIGYDLASSQ
                     EALLLARKYRQVHAVVGIHPHDAQHLNQDVLNKLYDMARDQRVVAIGEIGLDFYRDLS
                     PRDTQRQAFREQIKMAQEIGKPIVIHDRDAHQEVLEIIKAEKAGKNQGIMHCYSGGLP
                     LASELIKLGFYISFAGPLTFKNARKAHEVAAKIPMDRILVETDCPYLSPEPFRGKLNE
                     PANVKYVAAKLAELKNKSVDETAYLTGLNARKVYRIKD"
     gene            complement(6740..8704)
                     /gene="metG"
                     /locus_tag="P5549_06855"
     CDS             complement(6740..8704)
                     /gene="metG"
                     /locus_tag="P5549_06855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRW12826.1"
                     /translation="MSNKNTYYITTPIYYPSDNLHIGHAYTTVAADCMARFKRMQGRD
                     VFYLTGTDEHGQKIERAAREKGKEPIEHVDEIVANIRKLWDLLMISNTDFIRTTEERH
                     EKVVQKIFRKVYEQGDIFMSEYQGWYCSPCETYFTERQLVEGKCPDCGREVELLKEES
                     YFFNMAKYAPRLLEHIDTHPDFIQPESRRNEVVSFIKGGLEDLCVSRTSFSWGVPVPF
                     NRKHVVYVWFDALINYLTGIGYLEDEDQFQKYWPADVHLMAKDIIRFHAIIWPIMLMA
                     LDLPLPKKVVAHGWIMLEGGKMSKSKGNVVDPAILVSKYGIDAVRYYLLKELSFGQDG
                     LYSEEIMVTRINSDLANDLGNLISRTIAMIIKYFDGVIPSVDEKDALDEELRQMALTA
                     YKEATDKLDKLDFSAYLNAVSRLVSRANKYIDETEPWKLAKAETGKKRLATVMYHLVE
                     AIRIAVVLTAPAMPTLMERANLQLNIFDLEKTLLWEDGGIWGMTPAGAKVSRGEALFP
                     RIDMKTLEEDYVDEKEAASQPVTDEKTAPVAEPQEEKTYITIEEFAKMDLRVAEVIGC
                     EKMEKADKLLILRLKVGEEERTVVSGIAQHYTPEDLIGKKVVVVANLKPSKLRGVMSE
                     GMILAASDEGHHNLEVLTLQSELPSGNRIK"
     gene            9081..9329
                     /locus_tag="P5549_06860"
     CDS             9081..9329
                     /locus_tag="P5549_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRW12827.1"
                     /translation="MKSTGVVRKVDELGRIVIPIELRRTMGIEEKDALEIYVDSDKII
                     LKKYEPACIFCGNAEDVVNYKGKNLCKACLTELVKQIG"
     gene            complement(9340..10176)
                     /gene="rsmI"
                     /locus_tag="P5549_06865"
     CDS             complement(9340..10176)
                     /gene="rsmI"
                     /locus_tag="P5549_06865"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRW12828.1"
                     /translation="MQAGTLYVCGTPIGNLEDVSIRLLKTLRRVDLIACEDTRNTIKL
                     LNHYKIKKPLTSYHQYSGNERENHLLAKLLSGKSIALVTDAGMPGISDPGPELVKKAI
                     AADIKMEVIPGPSALTAALAISGLDSSSFIFYGFLPRQRSQRRNLLMELTNETRTAIF
                     YEAPHRLLSCLEDMQEVLGQDQPLMIGRELTKKYQEMQRGTIGGLLAYYQKQAPRGEI
                     CLLLPGRQKADGSPSLEQIAEETRQLIKKGLDKKEAFKMKAREYQIKKSDLYNYFLDY
                     PE"
     gene            complement(10225..10965)
                     /locus_tag="P5549_06870"
     CDS             complement(10225..10965)
                     /locus_tag="P5549_06870"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRW12829.1"
                     /translation="MLNDEIHPDESLDDLILGKMKVIQAREGYRFSLDAVLLAHFPAD
                     TGVRQVIELGSGSGVVSLLMAWRAPRAHITAFEVQPAMVERSRRSVLLNGLQERIDIM
                     EADIRETEEILPGGNAELVLSNPPFWRQGEGKVSRGAEEAVARHEIMMGLPQLVEKGA
                     YLLAPGGKMDIIHRAERLDEAMELFRLYKVPIRRLRFIHSFSDRTARLVLIEGVKYSS
                     GPVTIMPPLIIYEQTGVYSQELRSIYGK"
     gene            complement(10970..11266)
                     /locus_tag="P5549_06875"
     CDS             complement(10970..11266)
                     /locus_tag="P5549_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="initiation control protein YabA"
                     /protein_id="HRW12830.1"
                     /translation="MDETAKNVLELKTIIREMVLEVGELKERITRLESALQEQAEPES
                     VNIRKPDREIIELQGEGYERLGMLYKDGYHVCPSNFGDSRQEECLFCLAFINKE"
     gene            complement(11217..11921)
                     /locus_tag="P5549_06880"
     CDS             complement(11217..11921)
                     /locus_tag="P5549_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HRW12831.1"
                     /translation="MALVVGVRFKPAGKIYYFDSGDFELGKGDAVIVETVRGIEYGEV
                     ALGKREVEDDQLVLPLKKVIRVANEEDKSVYTSNKQKEKEALELCKEKVRLHNLPMRL
                     IDVEYTFDMGKIIFYFTAESRVDFRELVKDLASIFKTRIELRQIGVRDEAKMLGGIGT
                     CGRVLCCNNFLGDFEPVSIRMAKEQNLSLNPTKISGICGRLMCCLKYESELYEGKGDK
                     NGRNSQKCAGVEDHHS"
     gene            complement(11977..12933)
                     /locus_tag="P5549_06885"
     CDS             complement(11977..12933)
                     /locus_tag="P5549_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12832.1"
                     /translation="MGYFDKIIGQERAILPLQRSLASEHISHAYLFLGPTGAGKYTTA
                     LEMARALILGSDPQGEAYWREEVHPDFRIIEKAEKKSMIGIEQITNDIEPWLALKPYR
                     AKRRVVIIKDAQLLSLPAANALLKTLEEPPDYAVIILVADENNLLETIISRCQVIKFE
                     PLREEALTAYLEGQGMESSKAVHLARLAQGSITTALLFAEEQWHEIWGSAQDVLESLA
                     GGNPLEVFLAAEKIEKQPLIMVSLLETILRDVCVYQQTGNSKQLLMEHNLPFCQKCKH
                     LHPEQVGQALGRISSLKKMYRRSVNPVLLNINILYELQTALR"
     gene            complement(12993..13610)
                     /gene="tmk"
                     /locus_tag="P5549_06890"
     CDS             complement(12993..13610)
                     /gene="tmk"
                     /locus_tag="P5549_06890"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRW12833.1"
                     /translation="MKTNGKFISLEGIDGCGKTTLRDQLSDYLQGKYEILMLREPGGT
                     EVSEKIREMLLDVRNVGIRPHTEAFLYAASRSQLVEEVILPALQEGKVVIADRFMDST
                     IAYQGYGRGIDIEFLKVLNLLCTGGVKPELTLLLDIEPEEGQRRRDQQAPDRLENEGV
                     EFQRRVREGYLEMQKNEPERIKLINGGTGIIEVREQALQYLSVLL"
     gene            complement(13639..15069)
                     /locus_tag="P5549_06895"
     CDS             complement(13639..15069)
                     /locus_tag="P5549_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRW12834.1"
                     /translation="MQDQTPIFSALCRYSKADHLRLHMPGHAGGRGMNEMLQEVAAID
                     VTEVAGMDDFHLPQGIIAESQRLLARAVGAGESFYLVNGASSGIHALLMSMTADGDSV
                     LLPRNCHRSFYGGMVLSGAMPSYIPGRIDDEMGLELAVKAAEVEQCLAANPEVKAVFI
                     TSPSYFGTCSDVTAITELAERYDLAVLVDEAHGAHFPFHEDYPASALAQGATAAVNGL
                     HKTWPVLNQGGSLHVKKGFPEPARLHQAVSLLTTTSPSYPLLASMELARLFMEQEGHK
                     KLEQARRWSVEYRKKINQIKGLHCHADELKARTAISGIDPLKIVISLPGLTLNGYQLS
                     AILREQYHIQLEMETELVILAMFSPFHEVEDWERFYLALQEIAAGYAGKKNRPRQVEA
                     PPAPQVVLSPRRAYQSAKINVALAASRNMISGEMVAGYPPGIPCLLPGERISEEILDY
                     LFYLKRSGARIQGPSDAKLDHIMVIE"
     gene            complement(15123..16052)
                     /locus_tag="P5549_06900"
     CDS             complement(15123..16052)
                     /locus_tag="P5549_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRW12835.1"
                     /translation="MAAGNKVLFIVNPQSGGGRTSKQWKKVERWLRMKNYSFDVQPTE
                     RAMHACEIAGDALRKGYDHIIAVGGDGTINEVVNGFFLNHEDIRERAFLSVLPMGTGS
                     DFARVLPVSASADYVEKLLTDCQEMACDIIKTEYLGWNGSPQTRYFINVADVGVGCET
                     CIRLNKKSKVMGGFLSFLLTFLGVLFSFKSPEFTVEVDGELFYSGKSSLLAINNGKYF
                     GGGVMIAPQASLDDGLMDIMLLKDANTFDFLTALPSAYKGKHLDHPKIKLTQGRQVKL
                     SSAVHIGLEMDGETPGTGDAMIRIVPAGIKLFV"
BASE COUNT         4177 a         4151 c         3302 g         4792 t
ORIGIN      
        1 tatcggtctg cacagaaatt tgtcaggcca aatttctacg atatattatg aaaaaggtgg
       61 ggaaggtgtg attgatatgc ggaaagttaa tgaaattcag acttaaattt tatcaggcat
      121 tgttgataaa gaattcctta atttttttac ccatttgctt gcgtcgttcc tcccttgtat
      181 aaaaagaaat gatttttccc tcatcgtttt tgctttttct ctgtttggat tcatccatgt
      241 tgcggggaat aacgaaagct tcctctgctt gccgcagcaa gcgaatctgg cgttgcaact
      301 cggcatttac ggtctctgcc ttttgcagac tcttcttcaa gctgagcaga ttcatctcat
      361 agatcctaat ttgccgacgc agatcgatat tctccctttg cgatgcgttg aagaggttct
      421 cgtcggctgg atttaatccg gtcagatttg cctgaccaat tcgctctggt tgaagagaaa
      481 cgatctgctc ccgcaacgcc aggagcataa aagtcatatc ataggtaatg ataaggtttt
      541 gatcccgcag gattgaatct ccattgaacg cttttatctc tgctacatag accagttcct
      601 gtccggcctc cagctgtggc aggaactcgg ttaacgcaac actttcggta atgatgtccc
      661 ggcggctgaa aggaagcaga ttgccttgtt catcttccat cacaaacaac gtttccacat
      721 tcacttttaa ttctgctgcc cttcccttgc acgtaacctg acgtgcgctg attctggtct
      781 tcatctcttc gattctgcga aacccaacgg cggttctgca ttcacgtgta tgctggcaga
      841 tctttttttc cggcaagcgg gctactacca cggatgacca aatattgatt ctctcctgca
      901 aaagatcatc tcctcgataa ggatataaag caggcggctc aaaccctcca attattactt
      961 ctgcttttca tatttatatg gttgtgaccg gaatactatg ttattccatc tggctttaca
     1021 gttgtcagac caaccaacgt caggcaaaaa aaagctgcac ctttggtgca gcctgcaatt
     1081 ggtgtattta attattggca gaagcaattt ttatctcttc gttttttgcg ctatatacga
     1141 aaagttctac cgtttcagta agcacatctg cataactata agatactcgg cgatcaattt
     1201 ttctctcatt caatttgacg acaaatatgt tagggtaaat agattcgagt accccttcct
     1261 tctcaacgat gcggttcctg ccgcggttcg cctttaatct tatcctgctg ccaacgaagg
     1321 attccaggtc tttcttgatc tccgcgatcg ccctgggtga attaatcaaa ttaccaccct
     1381 ctttaccgat ttatctttta aatcatacct cattagtccc ccaaagtcaa tagcaaacaa
     1441 atagtatatt aaaaaacgaa ctccgtgtca acaagattaa tggggaattt aataaaatgc
     1501 tttgaccagc ttgatataat cgtaaaaatc caaattttcg ggccgcagag caggggccaa
     1561 agcaatcatt tgcagttttt cagcggcgat atttttgtcg atttgaaaaa tatttacgat
     1621 taaattcaaa atggtttttc ttctttgctg aaaaaagcca tttaaaattt gttgcaaaac
     1681 gatttcttca tccggcgcca atattttctt ggaatgggga ataaatttta atacaatcga
     1741 atccacttcc ggtttcggat aaaaacaatt gcgggatacc tggataactt tttccacttt
     1801 ggcgtgataa gctgcagcaa ccgtcaaacg cccgtaatct ttactgcccg gtttggccag
     1861 aatccgttcg ccgacttctt tttgcatcat caaagtcgct gactgcagat gagggcagct
     1921 ttccaacaat ttaaatatga tcggagtggt gatattgtaa gggatattgg cgcagacctt
     1981 gtaagtgacc atttcgttta attcaaattg cttttgcaat tcagcctcaa tatctgtttt
     2041 taaaatgtca ttaaaaagca gtctgacgtt gtcaaagcca gccattgttt cctgtagcac
     2101 tgcttccaaa cgctgatcga tatcaatggt taatagtccc cgtccgcgtc tggccagttc
     2161 tctgctgagc gctcccaggc ccggaccaat ctctacgatg tactggcttt tatcaagttc
     2221 cgcggcatca gcaatgcggg tcaatatatt ctgatcgacc agaaaatttt gtcccagacc
     2281 tttgcgagca tggatattga accgctgcat gatctcctgt actccttttg ccgatgccaa
     2341 aacaaccatt gcatatgctc ctttgttgct tgatggaaag aaagtctaac gcttattata
     2401 ctacacctaa aaaaatcctt ctcacaaacc atgtcagaag gatttatccg agctcagcca
     2461 taagctttta ttggcttgtc atcgcagagc gggtttattt tacttgttct ttcatcattt
     2521 ttcctagttc agctgcactg cgggcatagc taagaactgg ttttccttcg attaacccgg
     2581 ctgctacatc gttgattttt ttcaccatgt tggcatcaga tgtaaccaaa acatccgtga
     2641 ctcttttatc actgtccaga attttttgtt tcaccatttt tttagtactg gcctgctggg
     2701 ttgctgaagc catgacctca gtattaaggg ttatgccaac catgactacg attttgcccg
     2761 gactgctggt actgatttct ttggtggcat tcgtgctggt cccttttgca gcagcatcag
     2821 cgccggccac tacaaccgta gcattgacta ccccgggtac tgtcttggct agttcagaaa
     2881 aacgattggc catgatccgt ttatcagact ctgtgacctg gatgggatgt gccggagtcg
     2941 cgttgggatt cgcattgggg gttgagtagg gtttttgggc ttgctgacag cccgatgtca
     3001 gcagtattaa cgccatgaga acaaacccca gtaatttagc ctggctcatt attcttgcct
     3061 ccttgtcttg attaagtcaa tgatttattc ccgctgcttt gtggctgtat gttccttgat
     3121 atagttttaa ccggtgccca caattcaatc agagcaatat atggcaaaaa aagaagcgga
     3181 gaccctcgtc tccgctgcag tattgctgtt agtttaatac atatacctta actgaccttc
     3241 ggccccaatt tatacattgg gaataactct ccatgaataa atcaaccgtg ttgcctttga
     3301 tcgctccgcc ggtatcggca gcccgcgcgt acccgtagcc ttctatatac agacggctgc
     3361 ccatgggaat tacgttggga tcaaccgcta ccataccaac gctgggattc agaccggtgg
     3421 ccgttcgaaa gccagtatac gtatacgctg aagcttgcat gtatttggct tcacgaaaat
     3481 ccaaacgttc actgccacga gatacggatg taatactgcc catcgcaaca acccgattct
     3541 gaggctcctg caatgtttcg gtggaaatga tttccctttt ttcttcctga ccattataga
     3601 agattatctt aatggtattg cgtgcaatcc cttccttgcc tgccttgact gttttggtca
     3661 agcctcgttc cagcgtagga tcatcaatct gttcactgct atagggaatg gtctgatctt
     3721 cattcacaat ttgttctgtc actctgatga cttcaatttc ctgattggta ctaaccagtt
     3781 ttcctgcttt ggtcttgata atatcgtttt ctcccaattg aaatccagcc aaactgactg
     3841 ctttttcggc agtaactgga gtggtttcaa tcgtctttat tgtcccatcc gccaagaccc
     3901 ggactttaaa tgctctcgta actacgattt gggtgtgtct ggtgactttg ctatcgagta
     3961 tcggttgaac taaatcattt tttcccagtc ctatatggtt cttttgcaaa acatctttta
     4021 ctgttccgga aaacaaaaca cgtgtctgaa tgagctttcc atctgctgca atggtaactg
     4081 gcttttgcag cgcaaaaaac atactcgtca ctagaatccc cagtgtcagc aggctggcaa
     4141 tcaggatcgt gcctttcttt gaccaggcta aatccatact ttacctccct tgatctttat
     4201 tctcccgcag gagagcaata ctggtttacc caaaataaca aataaacagc caaaagtcaa
     4261 attccctccc ttaatactgg tttggctgcc atttaattgt tattcaagtg taaactatct
     4321 tctactttat atatttatca tcttttcatt attttattct ttaacaggta aaaagtttgc
     4381 agaattataa attttaccat ttattttttc cgttcacttg tctacatttt ctaccatata
     4441 attgaaggca cggattgatc tgaaaaagct gcacatcaat agaagcagtg tttttcattc
     4501 gttcgaaaaa ccaggaaggg tttttccaaa cagccgaaga aattaatgaa ttaaccgatg
     4561 attacaagga aggattgaga tatcgtgctg aacgatcgga aatcatcttt ttgggtggtc
     4621 gtatttgcag tggtggcagt tgtcatggta ggcatagctt tttttaccac ccccccgcga
     4681 gaggatacgg ttcaacagca agacataaaa tcaaaccctc cttccgttgc cccggaagga
     4741 gggttcacga ctcgttatga aaccgttaaa attgaatttc tcactgatat gaagggattt
     4801 aaatcaaccg accgatttac ttccaccgat ttagaaatag tagcttatat tgatgcaacg
     4861 ctcacaaaca gtcaaacggc tgtacaagca aatgacatga acaacaatca tacaaatcag
     4921 tatttaatcg aattgtctaa tgtcatcggc ggatacagct gtgggttgta ttacgatacg
     4981 ctgtatgaca aagcctatct ggtcaaggac ggcggccttt atgcaaccgg gacagatttt
     5041 gcccgttata ttgatgcttt gctggagaac accaagatca ttgcccatat agatgacact
     5101 gcggcggtga agctgtttca ggcctatgga tggacgcttg attacaagat caatacgcgc
     5161 ccagacaagt tgaacgatat tcgtgttttg aaggagtttc agcccaacgc atactatttc
     5221 gcttataaca atgaactttc caaggagatt ggccttgata tgagcagcta ttccaattct
     5281 cccaacatca atgttgatat ttacagaatt cacgaaagca tgccgcaaga attttatccc
     5341 atacagaatt gtcgcggtat agtgataaaa aaaggtaata aaatcatcgg cgcgtttatc
     5401 agcgccggtc gacacagcgc tttcaatgcc tgtagtttaa aagccaaccg ctttgaaaaa
     5461 gccaccggtc aaacacttga ccagtggctt gcccgcatga ttaaagcaga ccgcattgag
     5521 gaaaggctat ccaagttaga gccggaagag atgattgagg aatatttcat agcgcttgac
     5581 caaaagaata ccatagctgc tgcctattgt atctcgaaaa aaactctcct ggaaaatctg
     5641 acctccaaca tgctgaacga gcaattgtac aacgagaaca tttctctgcc tctaacggat
     5701 gctggtatcg aagcgaaatc aagttttacc aatctggaat cagccaatct attgcatatt
     5761 gaaccaatgg atagctctga taagaatacc aaaatctttc gcgtaaccgt ggatctccaa
     5821 taccatcaag cattgacgat cggcaacggt cagcagcttt gggattgcag tatggttttt
     5881 gaatcccctc agaccggatg gaaaatagcg gggttcggac attaaagacg acgacctcct
     5941 caccccttca ctaatccttg atccgatata cttttctggc attcaaaccg gttaaatacg
     6001 ctgtctcgtc aacgctttta tttttcaatt cagccagttt tgcggctaca tatttgacat
     6061 tggcgggttc gtttaatttg cctctgaatg gctccggaga cagatacgga caatccgttt
     6121 cgaccagaat ccggtccatg ggaatcttcg ccgccacttc atgagcttta cgagcattct
     6181 tgaaggtcag gggcccggca aaagaaatat aaaatcccag tttaatcagt tccgaagcca
     6241 gcggcaatcc gccagaataa cagtgcatga tgccctggtt tttgccggct ttttctgcct
     6301 tgatgatctc caaaacctcc tgatgggcat cccggtcatg gatgacgata ggcttgccaa
     6361 tttcctgagc cattttgatt tgctcgcgga aggcttgccg ctgggtatca cggggcgaca
     6421 gatcgcgata gaaatccagt ccgatttcgc cgatagcaac cacgcgctga tctctggcca
     6481 tatcataaag cttgtttagc acatcctgat tcaggtgttg cgcatcatgc ggatgaattc
     6541 ccaccactgc atgcacctga cgatatttgc gggccagcaa caaggcttcc tgagaagagg
     6601 ccaggtcata gccaatacag attatttttt ctactccggc ggcctgggcc cggtttaaaa
     6661 cctgatctaa ctcctttttc agatcttcgt cctgtaaatg ggcatgtgta tcgattaaca
     6721 tggttttctc cctgccgatt tatttgatcc ggttgccaga aggcaactcg gactgcagtg
     6781 tcaatacttc caggttgtga tggccctcat cggaagcagc caggatcatg ccctcggaca
     6841 taacacctct tagtttgctg ggtttcaaat tggcaaccac cacgactttt ttgccgatta
     6901 ggtcttccgg tgtataatgc tgtgcaatac ctgacacaac cgtgcgttcc tcttcaccca
     6961 ccttaagtct gaggatgagc agtttatcgg ctttctccat cttttcacag ccgatgactt
     7021 cggctacacg caagtccatc ttggcaaatt cctcaatggt tatgtaggtt ttctcttctt
     7081 gaggttcagc aaccggcgca gttttttcgt ctgttaccgg ttgactggca gcttcttttt
     7141 catccacgta gtcttcctcc aatgttttca tgtcgatccg cgggaacaaa gcttcgccac
     7201 ggcttacttt ggcgcccgcc ggggtcattc cccaaatccc gccgtcttcc cacagcaagg
     7261 tcttttcgag atcaaaaata ttgagctgga gattggctct ctccatcaag gttggcatgg
     7321 ccggtgccgt caatactacc gcaatccgaa tcgcttcgac cagatgatac atgacggttg
     7381 ccaggcgttt tttccctgtt tctgctttgg ccagtttcca gggttcggtt tcgtctatat
     7441 atttgttggc tcgggaaacc agcctggaaa cggcattaag ataagcagag aaatcgagtt
     7501 tatccaattt atcggttgct tctttatacg cagtaagagc catttgcctc agctcttcat
     7561 caagcgcatc cttttcatca actgacggga tcacaccgtc aaaatacttg atgatcatgg
     7621 cgatggtgcg gctgataaga ttccccaaat cattggccaa atcggaattg atccgcgtaa
     7681 ccatgatctc ttctgaataa agcccgtcct gaccaaaaga caattctttg agcagataat
     7741 aacggaccgc atcgataccg tatttgctta ccaaaatggc ggggtcaacc acatttccct
     7801 tggattttga cattttaccg ccttccaaca tgatccagcc gtgggccacg acctttttgg
     7861 gcaggggcaa atccagcgcc atcagcatga tcggccagat gatggcatga aatctgatga
     7921 tatccttggc cataagatga acgtcagctg gccaatactt ctgaaactgg tcctcgtcct
     7981 ccaaataacc gatgccggtc aaataattga tcagggcatc aaaccagaca tacaccacat
     8041 gcttgcggtt aaaaggaacc ggtacgcccc agctgaaact ggtccgggaa acgcagagat
     8101 cctccagacc gcctttgata aaactgacga cctcattgcg ccgtgattcc ggctgaataa
     8161 aatcgggatg ggtatcaata tgttcaagca accgcggtgc gtatttggcc atgttaaaga
     8221 aataactctc ttctttgagg agttccactt cccgaccgca atccggacat ttcccctcga
     8281 ccaattggcg ttctgtaaaa taggtttcac aagggctgca ataccatcct tgatattcgg
     8341 acataaagat gtcgccttgt tcgtaaactt tccggaaaat cttttgcacc actttctcgt
     8401 gacgttcttc tgtggtacga ataaaatcag tattggatat catcaacaag tcccataact
     8461 tgcggatatt agcaacgatt tcatctacat gttcaatcgg ttctttgccc ttttcgcgtg
     8521 ccgcccgttc gatcttctgt ccgtgttcat cggttccggt cagataaaag acatcccttc
     8581 cctgcattct tttgaaacga gccatgcagt ctgccgctac ggtggtatag gcatgaccga
     8641 tatgcagatt gtcactggga taatagattg gggtcgtgat gtaatacgta ttcttgttcg
     8701 acactcctgt ccctccatta cacttcctgc tctggagcag ttctaaccca caacagagca
     8761 aaatctcaaa gtttattcgt atattttacc acaaattaac gttgctattc ttgattagat
     8821 tatcttgaaa gaggattttg tcggattact gcttttactt tcatgcttga tttcaatttt
     8881 cttgcacttt tgtcggtctt ggtattataa taagttcgtt actatgcgca ataagtaatt
     8941 taatgtttca taagttaaaa ttttgattgt ttttgtagtt taacccttga ctatttctac
     9001 cagcgctggt atactctagt tcaggacagg tcgaaagatg tactattcat tttaacaaag
     9061 attggaggtc tgcgaatatg atgaaatcaa ccggtgtggt tagaaaagtt gatgaattag
     9121 gtcgtattgt tatccctatt gaattgagac gcaccatggg catagaagaa aaagatgctc
     9181 tcgaaattta cgttgacagt gataaaatca tcctcaagaa atatgaaccg gcttgtatat
     9241 tctgcggcaa tgctgaagat gttgtaaatt acaaaggcaa aaatctctgc aaagcctgcc
     9301 tgacagaatt agtcaagcaa attggttaat ctcctcattt cattccggat aatctaaaaa
     9361 gtaattatat aaatccgatt tttttatctg gtattcacga gccttcattt tgaaggcttc
     9421 ttttttgtcc aagccttttt tgatcaattg tctggtttct tcagcaattt gctcgaggga
     9481 aggggaacca tcagcctttt gtctgcccgg caggagcagg cagatttcac cgcggggagc
     9541 ttgcttttga tagtacgcaa gcaacccccc tatcgtaccc cgctgcattt cctgatactt
     9601 tttggtcagt tcccgaccaa tcattaaagg ctggtcttgg cccaagacct cctgcatatc
     9661 ttccagacag gacaataaac ggtgcggcgc ttcatagaaa atggctgtcc gggtttcgtt
     9721 ggtcaattcc atgagcaaat tacgcctttg gctgcgttga cggggaagga atccgtaaaa
     9781 gataaagctg ctgctgtcca gtccagaaat cgccaacgcg gcggtaaggg cagacggacc
     9841 tggaatcact tccattttta tgtctgcagc aatagccttt ttgaccagtt cgggaccggg
     9901 atctgatata cccggcatgc cggcatcagt taccagtgca atactcttgc cgcttaacag
     9961 tttcgccagc agatgattct cccgttcatt gccactgtat tgatgatagc tggtcaatgg
    10021 ctttttaatc ttatagtgat tcaagagttt tatcgtattg cgggtatcct cgcaggcaat
    10081 caggtcaacc ctgcgcaggg tttttagcag acggatactg acgtcttcca ggttgccgat
    10141 tggcgtcccg catacatata gcgtgcctgc ttgcatcctt ttttctcctt gtataacttc
    10201 tgtcattttc tctttccctg gcatctattt gccgtatata cttcgcaatt cctgactata
    10261 aaccccggtt tgttcgtaaa tgatcaatgg cggcataatc gtcaccggac cggaggaata
    10321 cttgactcct tcgattaaaa ccaaccgtgc tgtgcggtca ctgaaggagt gaataaaccg
    10381 caatctcctg atgggtactt tatacagacg aaacaattcc atggcctcat ccaagcgctc
    10441 agcccggtga atgatatcca tttttccgcc cggcgccaga agataggccc ctttttccac
    10501 cagttgcggc aatcccatca tgatttcatg gcgggctacc gcctcttcgg caccacgact
    10561 gacttttccc tctccttgtc tccaaaaagg agggttgctc aaaaccagtt cagcgttccc
    10621 tcccggcaat atttcttctg tttctctgat gtcggcttcc atgatatcaa ttcgttcttg
    10681 cagaccgtta agaaggacac tgcgacgcga tctctcaacc atggcgggtt gaacctcaaa
    10741 ggctgtgata tgtgcccggg gtgctctcca ggccatcagt aacgatacca caccactgcc
    10801 tgagcccagt tcgattacct gcctgactcc ggtatcagca ggaaaatgcg ccaagagaac
    10861 ggcgtccaga gaaaagcgat acccttctct ggcttgaata accttcatct tgccaagaat
    10921 cagatcatcc aggctttcat cagggtgtat ttcatcattc agcaaacgct cactccttgt
    10981 taataaaagc cagacagaag aggcactctt cctgccgact gtcgccaaaa ttggacgggc
    11041 aaacatgata tccatccttg tagagcatgc ccagtcgctc gtaaccttcc ccctgcaact
    11101 cgatgatttc cctgtcaggc ttcctgatat taaccgactc cggctctgcc tgttcctgta
    11161 gagcgctttc caagcgggta attctttcct ttagctctcc cacttcaagt accatttcac
    11221 gaatgatggt cttcaactcc agcacatttt tggctgtttc gtccattctt atctcccttg
    11281 ccttcgtata gttcagattc atatttcaaa caacacatca ggcgtccgca gatccccgaa
    11341 atctttgtcg gattcaaaga aaggttttgt tccttggcca tgcgtatgga aaccggctca
    11401 aaatcaccca ggaaattgtt acagcaaaga actcttccgc aggtaccgat tccccccagc
    11461 atcttggcct catctcttac cccaatctgt cttaattcaa ttcgggtctt gaaaatggat
    11521 gccaaatcct tgaccaattc ccgaaaatcc acccggcttt cggccgtaaa ataaaagata
    11581 atcttgccca tatcaaaagt atattcgaca tcaatcaaac gcatgggcag attatgcaaa
    11641 cgcacctttt ccttgcacaa ttccagggct tccttttctt tttgtttgtt gctcgtataa
    11701 acgcttttat cttcttcatt ggcgacacgg attacttttt tgaggggcag caccagctga
    11761 tcatcctcaa cttctcgttt gccgagggca acttctccat attcaatccc cctcaccgtt
    11821 tccacgatga ctgcatcccc ttttcccaat tcaaaatctc cactgtcaaa ataatatatc
    11881 ttgccggccg gcttaaatct cactccgaca accaacgcca cggttatccc tcctaaccat
    11941 atttatattc acctgactcc aaaccatctt gtcttcctat cttaacgcag tctgtaattc
    12001 atagagaatg ttaatattca gcaaaactgg gttaactgag cgacgataca ttttcttcag
    12061 gctgctgatc ctgcccagcg cctgcccaac ctgctcggga tgtaaatgct tgcatttctg
    12121 acaaaacggc agattgtgtt ccatcagtaa ttgtttagaa ttgccggttt gttgatatac
    12181 acaaacatct cgcagaatcg tctccagtaa agataccata atcagcggct gtttttcgat
    12241 tttttccgcc gccaaaaaaa cctccaaggg attcccgcca gccaatgatt ccagcacgtc
    12301 ttgagccgat ccccagatct catgccattg ttcctcggca aacaacaaag ctgtcgtaat
    12361 gctgccctgc gccaatctgg ccaaatgaac tgctttggaa gactccatgc cctgaccctc
    12421 cagataggcg gttagcgctt cttcccgcaa cggttcgaat ttgatcacct gacaacggga
    12481 aatgattgtt tccaatagat tattctcatc cgctactaaa ataataaccg cgtaatcggg
    12541 tggttcttcc agcgttttga gcagcgcgtt ggcagctggc agcgacaaca gctgcgcatc
    12601 cttgatgata accacccgac gcttggcccg atagggtttt aaagccaacc aaggctcaat
    12661 gtcattggtg atttgctcaa tcccgatcat gctctttttt tctgcttttt caatgattcg
    12721 aaaatcagga tgtacttctt cgcgccaata cgcttcacct tgcggatcac tgcccaggat
    12781 caaggctcgg gccatttcca gggctgtcgt gtatttgcct gctccggtcg gacccaaaaa
    12841 aagataagca tgacttatat gctcgctggc aagcgatctc tgcaacggta atattgcccg
    12901 ttcctgtccg atgatcttgt caaaataacc catttcacac ctcagcagcg atttttccct
    12961 ttgcgtctgc tgcagatttt tcttccctgt tactataata acacagacaa atactgtaaa
    13021 gcttgttccc gcacttcaat gataccagtt cctccattga tcagtttaat ccgctcaggt
    13081 tcgttttttt gcatctcaag gtacccttct ctgacccgac gttggaactc cacaccctca
    13141 ttttccaaac gatcgggagc ttgttggtct cttctccttt gaccttcctc gggttcgata
    13201 tccagcaaaa gggtcaactc cggtttcaca ccacccgtgc ataaaagatt cagcactttg
    13261 agaaactcta tatcaatccc tcggccgtag ccttggtagg cgatggtcga atccataaaa
    13321 cgatcggcga tgacgacttt accttcctgc aaggcaggca ggattacctc ttccaccagt
    13381 tgactgcggg acgccgcata caggaaagct tctgtgtggg gcctaatccc tacgttgcga
    13441 acgtccagca acatctctct gattttttcc gatacttctg ttcctcccgg ttcccgcagc
    13501 ataagaattt cgtattttcc ctgcaggtaa tccgacaact gatcgcgcag cgtggttttc
    13561 ccacaaccat cgatcccttc cagactaata aactttccgt ttgttttcaa ttcctcaccg
    13621 cctcgagcaa ttcatgtctt attctatcac cataatatga tcaagtttcg catcactggg
    13681 tccttggatg cgcgctccag atctctttaa ataaaacaaa tagtccaaaa tctcttccga
    13741 aattcgttcg cctggaagca ggcacggaat tccgggtgga tatcccgcca ccatttcccc
    13801 ggaaatcata ttgcggcttg cagccagtgc aacatttatt ttagcagatt gataggccct
    13861 tctcgggctt aaaacgactt ggggggcagg tggtgcttct acctgccggg gtctgttttt
    13921 cttgccggca taaccggcag caatctcctg caaggccaga taaaaacgtt cccagtcttc
    13981 aacctcatgg aaaggggaaa acattgctaa aatcaccagc tctgtttcca tctccaattg
    14041 aatatgatat tgttccctta aaatggctga caactgatag ccgttgagcg tcaacccggg
    14101 caacgaaatc actattttca aaggatcgat cccgcttatt gctgttcttg cttttaattc
    14161 atcggcatgg caatgcagac ctttgatttg attgattttc tttctatact ccacagacca
    14221 tcgtctggcc tgctctaatt ttttatggcc ctcttgctcc atgaacaatc tagccagttc
    14281 catggaagcc agcaaagggt aggacggact cgtcgttgta agcaagctga ctgcctggtg
    14341 caatcgtgcc ggttcgggaa aacctttttt aacatgcaga ctgccgccct gattcaagac
    14401 cggccaggtc ttgtgcaagc cgtttacagc tgcagttgcg ccttgtgcca gcgcggaagc
    14461 aggataatcc tcatgaaaag gaaaatgcgc gccatgcgcc tcatccacca gaacagccag
    14521 atcatatcgt tctgccagct cagtaatcgc ggtaacatca gaacaagtcc caaaatagct
    14581 gggactggta ataaataccg ccttcacctc tggattggct gccaggcatt gttccacttc
    14641 tgccgctttt accgccagtt ccagtcccat ttcatcgtct atccgaccgg gaatatagct
    14701 tggcattgct cctgacagca ccattccacc gtagaaacta cgatggcagt tgcgcggcaa
    14761 cagaacggaa tctccgtctg cggtcatgct catcaacaac gcatggatgc cactgctggc
    14821 tccattgacc agataaaagc tttccccggc accaactgct cgcgccaaca aacgctgtga
    14881 ttcagcaatg attccctgcg gcagatgaaa gtcgtccatg cctgctacct cggtaacatc
    14941 tatcgctgca acttcctgca gcatttcatt catgccccgc ccccctgcat gcccaggcat
    15001 atgcaggcgc aaatggtcag ccttgctata gcgacaaagc gctgaaaata ttggcgtttg
    15061 gtcttgcata gattacttcc ttgtttgaaa attgtccctg ccttgtagcc tgtatccctg
    15121 cctcagacaa atagctttat cccagcaggt acaattctaa tcatggcatc tcccgttccc
    15181 ggggtttcac catccatttc cagaccaata tgtaccgcag aagacagttt cacctgtctt
    15241 ccctgggtta gtttgatctt gggatggtca aggtgtttcc ctttatacgc actcggcaaa
    15301 gcggtcaaaa aatcgaatgt gttggcatct tttaaaagca tgatatccat taacccgtca
    15361 tccagcgaag cctggggagc aatcatcacc cctccgccaa aatacttgcc gttgttgatc
    15421 gccaacagac ttgatttgcc ggaataaaat aattctccat ccacctcaac tgtaaattcc
    15481 gggcttttaa acgagaagag cacccctaaa aatgtcagca aaaaagataa aaatccaccc
    15541 ataaccttgc tctttttgtt aagtctgata caagtctcac agcctacccc cacatcagct
    15601 acgttaataa aatagcgtgt ttgggggctg ccattccagc ccaaatattc agttttaata
    15661 atatcacaag ccatctcctg acagtcggtt aggagttttt ccacataatc agcactcgcc
    15721 gaaaccggta ataccctcgc aaaatcactg ccggtaccca taggaagaac ggataaaaat
    15781 gctctttctc ttatatcctc atgattcaaa aaaaaaccat tcaccacctc gttgatcgta
    15841 ccgtcaccac cgacagcaat aatatgatca taaccttttc gcaaagcatc tccggcgatt
    15901 tcgcaggcat gcatggctct ctccgttggt tgtacatcaa aactataatt cttcatacgc
    15961 agccatcgtt caaccttttt ccattgtttg ctggttcttc ctccacctga ttgagggttg
    16021 acaataaaca gtactttgtt gcctgctgcc ataaactctc tccacacccc aacaatttat
    16081 ttacccacat taaccggttc atatctcttt tatattctat atgaaaagat acaacttccc
    16141 aaataaaaaa taggcgcgca gcgggcctat taaaagtgaa ctatttaatt ctttccactc
    16201 cgggcaccaa aggtgcctgg cctcctcctt taaatgtagc ccgttatttc caataaagtc
    16261 tttaagtgac tccggttttc catccgggat cgatcacatc ggatatggat cgtcacttcg
    16321 tcaatccata tcacgatctg tcagaataaa ataattcctg gcaacgtcct actctcccac
    16381 agaatctgca gtaccatcgg cgcaggagag cttaactgcc gt
//
LOCUS       DAOTHZ010000049        15958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4421_length_15958_cov_7.007546, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000049 DAOTHZ010000000
VERSION     DAOTHZ010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 15958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15958
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4421_length_15958_cov_7.007546"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1419)
                     /gene="rlmD"
                     /locus_tag="P5549_06905"
     CDS             complement(163..1419)
                     /gene="rlmD"
                     /locus_tag="P5549_06905"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRW12836.1"
                     /translation="MQCLVEGISHAGEGVARIAGKATFIPYAIPGETVAVTIIEEKKS
                     YQRALLQQVIHPSQDRCQPNCRHYFTCGGCSFQHVDYPRQLTLKRQIVEETLRRVGGI
                     EIPVSPVIGMETPWHYRNKVEWHLSSQSGREMFGYFMNQSHEMVDIENCPLISQAMQA
                     VSLYIKNHRKILRVPDKCEITVRQSSLGQIMIIFSGNQTSQIDFALLADHCKEISFYS
                     IDQNITRLHYGETRLEEKLGEFKFEISPLAFFQVNHQQNEKMLAVIKDYARLQRSDSL
                     LDAYCGTGSIALSLAREVKRVVGVENFKAAIKDAKRNAFLNNITNTQFIKGACEEIVP
                     ALTDDFDVIILDPPRSGCKKELIQEVINKSPRSLIYVSCNPATLARDLALFEDSVYQV
                     KEIQPIDMFPQTSHVETVVLMSKVDK"
     gene            complement(1448..1996)
                     /locus_tag="P5549_06910"
     CDS             complement(1448..1996)
                     /locus_tag="P5549_06910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRW12837.1"
                     /translation="MSNEIKILGLSGSLRKQSYNTAALRAAKELLPSGAALEIADLSM
                     IPFFNEDVEAEGMPAAVADLRQKIAAADALLLATPEYNYSIPPVLKNALDWASRGNDN
                     PLNGKLAAIMSASPGMFGGARAQYHLRQVCVVLNLQLFNKPEVFIMKAHEKFDAEGKL
                     TDEYTRNAIARLLQALVDQVKQ"
     gene            complement(2054..2638)
                     /locus_tag="P5549_06915"
     CDS             complement(2054..2638)
                     /locus_tag="P5549_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HRW12838.1"
                     /translation="MQDKVTNIFIVGVGGQGTLLTSRIIAQVAVQMGFDVKVSEIHGM
                     AQRGGSVVSQVRYGKKVYSPIIKKGDADIILAFEKIEAARWLDYLKPDGMVIINNERV
                     NPLPVMSGKEKYPDTIHDKIAGLLPKTAIIDATAIAADCGNVKAANVVLVGMLTAAIG
                     IPAEEVEKAISAIVPAKALQVNLSAFRKGLAAGA"
     gene            complement(2643..>3689)
                     /locus_tag="P5549_06920"
     CDS             complement(2643..>3689)
                     /locus_tag="P5549_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRW12839.1"
                     /translation="TSGVPYQYVKEAFPQASVLKLGLVNPLPQELIKSFASQVDKLVV
                     VEELEPVIEEQVKSWGIEVSGKEQLTLLGEYSAEYLQQQLGGVPMPEGFNLDKQVPAR
                     PPLLCAGCPHRGVFHVLHKMRLVVAGDIGCYTLGAIAPLEGMDTCICMGASIGTAMGM
                     EKARGPEFAAKTVAVIGDSTFVHSGITGLIDVVYNGGTSTVLILDNDTTAMTGHQDHP
                     ATGVTITKQPTNKLDIEALVKAIGVKNLRVVDPLKIDELELALKEELARREPSVIICK
                     RPCVLLPGTPKPDHKIYVDAELCTGCKSCIRVGCTGVTFLDEEKVAQINDGCIACGLC
                     EQVCKFDAIKWMEV"
     assembly_gap    3690..3789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3790..4244)
                     /locus_tag="P5549_06925"
     CDS             complement(<3790..4244)
                     /locus_tag="P5549_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRW12840.1"
                     /translation="MKELMIGNAAIARGAYEAGATVATAYPGTPSTEIVSSFAEFPDV
                     YAEWAPNEKVALEVGAGASIAGARTLVAMKHVGINVAADPLYSFSYTGVNGGLVIVSA
                     DDPGLHSSQNEQDNRGYGRFAKMPVIEPSDSQEALDFTKLAFEISEQFDT"
     gene            complement(4369..4905)
                     /gene="yyaC"
                     /locus_tag="P5549_06930"
     CDS             complement(4369..4905)
                     /gene="yyaC"
                     /locus_tag="P5549_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HRW12841.1"
                     /translation="MIWVDTDYRDSLTRRLLVDALTICQAGETKLPVFLCIGSDRHLL
                     DCFGPLTGTLLQERLPQLNVYGTLDQPLHARNLTREMIIIKNRHPRQKYIAIDAAVGE
                     EHEIGRLQLRNGPIYPGKATAKSLPAVGDYALTAILEKRGHKAAAGRGNSPGFAAVYH
                     MAKIVQQAVADWYDLYPQ"
     gene            complement(4902..6014)
                     /gene="hisC"
                     /locus_tag="P5549_06935"
     CDS             complement(4902..6014)
                     /gene="hisC"
                     /locus_tag="P5549_06935"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRW12842.1"
                     /translation="MQDFVKRKARQEIFQLKPYIPGKPIEEVKRELGIDNIIKMASNE
                     NPLGPSPLAMQAITKALPDIYLYPDGNCYELKRGLAAFYQIPANQIIIGNGSDELLKL
                     IAEAFICKDDEVIFAQPTFSEYEFVSLIMGAQCIKIPLQDYTLDLQGVLRAVTPKTKM
                     IFICNPNNPSGTMLGEQATRQFMEQVPDDIIIVFDEAYVEYVESPDFPDSLAYVHQGR
                     NVIVSRTYSKIYGLASLRVGYAMTTASIAQAIETVMEPFNVNGLAQAGALAALADKAH
                     VAKSRQLNKEGKQYLYREFTRIGLEYVTTQANFILVDCGYDCQEVFRELLQRGIIVRT
                     CDHFGYPHAIRITVGRPEDNQRLINGLEQVLRKLKQ"
     gene            complement(6017..6883)
                     /gene="speE"
                     /locus_tag="P5549_06940"
     CDS             complement(6017..6883)
                     /gene="speE"
                     /locus_tag="P5549_06940"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRW12843.1"
                     /translation="MNGSWIYEKHTENYQVGWKIDHILHQEKSEFQEITVADSEQWGR
                     MLLLDGVVQTTEKDEFMYHEMITQVALHAHPQVKKVLVIGGGDGGTLTQIIKHPTVEH
                     IDLVEIDRAVIATCKEYFPCFQAGFSDPRTHIIIRDGIDYLKKTDQKYDLVIVDSSDP
                     TGPAVGLFTEAFYQTIYQALERDGLMVTQSESPLFYEDIFREIYRNITLVFGQAYIYH
                     STVPTYISGPWTFTVGSKKYDPRSSRFNKPNANDLKYYSCAIHQAAFVLPPYIEKLLS
                     DTGEPIFASGVK"
     gene            complement(6904..7230)
                     /locus_tag="P5549_06945"
     CDS             complement(6904..7230)
                     /locus_tag="P5549_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12844.1"
                     /translation="MNGMDKNQKKVYDATLAMISRLHFQQALLSQEEMYFLLSLLDKI
                     AHGEMPHELAICLQQWQEGNWSDDINAIIKASLLPLDFNDPAAVKHTCGLIADLFNDQ
                     YDGEND"
     gene            complement(7230..8597)
                     /locus_tag="P5549_06950"
     CDS             complement(7230..8597)
                     /locus_tag="P5549_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRW12845.1"
                     /translation="MAILESSQYNQSLFDLESERRVLSAMLHAESACIEAYNSLKASD
                     FFSPLHATVFELTTSLYEREVHPTYVELIKEGHSLGVFANQRDMDELSYIAEHYIDDE
                     NIIYWIKRVKDKSRLRRFEMFLRKNYQLLQEENDQDVEQTLMLAEEELTNLTALEIDD
                     RIDNPADLARLGYEEVERRFLRFKEIKEKHKGVIPLDGLPTGFDNLNKVTLGYKPGDL
                     IILGAQTGHGKTSFALHTAAAIAVQERHNVIYLNTEMSREQIALRWGSILSGIEHDKV
                     RSGDINESELSRILHAYARLGESGFYSYPCPNLTPEKTISIARKFKTQKHIDIMIIDY
                     VGRMEKQDPKLQEWQILEQIVKTQKMLAQNLKIVVMCLVQLNPDGTLQGAKRMKNECD
                     LMLKLTPISKEEMEESDELKKYDMMPNYAIYIDKNRDGQSGIRIPIYFDLKRQTMMDV
                     KRVSI"
     gene            complement(8682..9854)
                     /locus_tag="P5549_06955"
     CDS             complement(8682..9854)
                     /locus_tag="P5549_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRW12846.1"
                     /translation="MSNDYAALEVYRWGFAHLPGAVFYYGKELELNLEDIGFLAALFY
                     AYEQSKPLFQKGLQIGQLMVCCPILNTQKISRKITQLSKLGLIEIQDGKSKSFADKTI
                     FLEPLMERLQQLIIRDHPLHISNQEEIGSAALEKYQQKIVQLELQLQEEKSKHTDAGI
                     NHGESAQESANYRIVADFIAKKSGNLLSVKMENELQKWLDDLGFTPEFLLCMLEMTFE
                     RSIHNPRDITKIAKDLKNYSVNTVEGLESYFGKYVDSNRSNLHTSQFDPDIATFGTFT
                     GIDMSAEARKKMYYKWRYDWRFSHPMIMKAGEIMCQRTKNGGLEYIDSVLHNWLSKEI
                     RSVDEAEKEIVDFKKRNKSTAKSMLLNGGKASSMNSSQDYEIYVPPEVLEELKSKA"
     gene            complement(10049..11632)
                     /locus_tag="P5549_06960"
     CDS             complement(10049..11632)
                     /locus_tag="P5549_06960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW12847.1"
                     /translation="MKIKFLGAARTVTGSMHVIECGGLCFAMDCGLFQGSKEIKERNY
                     QDFKVNPQTLDFVILSHSHIDHIGLVPKLCKLGFKGNIYCSFATAELAKVMLPDSAHI
                     QETEVERKNRKLLRAGKIPLEPIYTVEDAMKCLSQFRPVNYDELFTPAEGVEVRLRDA
                     GHILGSSIVELWLEEAGKRSKIVFTGDLGQTNQPFIKDPTFIESADYVVMESTYGDRF
                     HKDVLNRVEKLKQVIDETMKKGGNLIIPAFAVERTQDLLYDLNELYVKGDLDESIDVY
                     IDSPLAIAATEIFKKYTQLFDNETKNFVNNGFHPLELPHMKYSHSQEESMELNNINRS
                     IIISASGMCEAGRIKHHLKYNLWRPESTILFVGYQAEGTLGRKILDGEKIVTIFGEQV
                     TVKADIRQIEAYSSHADQSGLLSWLKRYDGTPKTVFLVHGEEKAQQTLAELIRRDIHL
                     QVVIPQWLDEYPLDESMHIEPKAVPMQTETRITNRSLEAEELYLQLRMKLNRFYQDSI
                     TGQKYEELIDKMEKITSQL"
     gene            complement(11667..12143)
                     /locus_tag="P5549_06965"
     CDS             complement(11667..12143)
                     /locus_tag="P5549_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12848.1"
                     /translation="MFGDLSSEEVELLNAYSLLGHATRKELRDYMRYLLGKHYKQELI
                     ASIFNNQLLHSLLHSLLHISEREDFDKNQIEKRALQFKEIYFAIFEQIHSKYADLVDD
                     LDSCEIVKDFGINSVDNILQACWANNRSAILFEVIELSESFNKFAKKKSARKIVAV"
     gene            complement(12280..13455)
                     /locus_tag="P5549_06970"
     CDS             complement(12280..13455)
                     /locus_tag="P5549_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRW12849.1"
                     /translation="MSSMQHHIADTVQSLKPSGIRKFFDLVSQTEGVISLGVGEPDFV
                     TPWHIREACLYSLESGYTMYTSNWGLPELREEIAQYEKRAIGVDYSPQNQILVTVGVS
                     EAIDIALRSLVEPGDEVLIPEPCYVSYAPGVLLAYGTPVPLACYAQHQYRLQPEELEA
                     KITPRTKVLILAYPNNPTGAIMEKQDLEALREIIIKHDLIVISDEIYAELSYHTRHVS
                     IAALPGMYERTIVMNGFSKAFAMTGWRIGYACGHQDIIAAMTKIHQYTIMCAPIMAQK
                     AAIEALRHGETEMRKMVADYDYRRKLMFNGLNEIGLSCFEPQGAFYCFPSITSTGMNS
                     EDFSEKLLFEEKVAVVPGTAFGESGEGHIRCCYAASVKNIEAALQKMQSFVIRHRKG"
     gene            complement(13452..13940)
                     /locus_tag="P5549_06975"
     CDS             complement(13452..13940)
                     /locus_tag="P5549_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRW12850.1"
                     /translation="MDIERRILELLENDARLSAKTIAAMLDTDEKVVADIIAQLERDK
                     TILGYRTVVNWENTEWEGVTAMIEVKVSPEREVGFNSVAERIYRFPEVRSVYLMSGGF
                     DLTVIVEGNSLKDVAHFVTHKLSPLDKVQSTVSHFILKRYKEDHIIFGENEEDKRLVV
                     SP"
     gene            14341..14571
                     /locus_tag="P5549_06980"
     CDS             14341..14571
                     /locus_tag="P5549_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12851.1"
                     /translation="MIKTDQLNQEERIVFERINEYFKSPAMTAQEKIINALLIAQLEL
                     DDHHFCNEKERLKIIHFQKTLDSLLGKIVNQS"
     assembly_gap    14576..14675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14932..15831
                     /locus_tag="P5549_06985"
     CDS             14932..15831
                     /locus_tag="P5549_06985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRW12852.1"
                     /translation="MKNLLANKSYKFIFEHSSDAILLTKTDGSIHLANPAACSMLHRT
                     AQEICQIGRNGVVDLQDPRLVTALQERDSHGNVRCELNFVRADGVKIPVEITSNVLAD
                     KEDSHWNVMIIRDMTVYKELEESYKRSHEQALQMCVLDFLTGIYNRRGFMERLQQELE
                     RSKRDGLQISIIIADLDNFKQVNDVYGHLAGDYVLKQFADCLNQNVRPYDILARFGGD
                     EFIIGLPGTGLLEATNVAERLRSISENLVITVDFQAIDLTACFGVTAYRKDVQDDLDT
                     LISRADDAMYKAKKTKNAVFAKE"
BASE COUNT         4007 a         3831 c         3431 g         4489 t
ORIGIN      
        1 ctaaatcgac cacacgcaaa cccatgttat ctaaatcgct ctgtcacgtt caaaaccatg
       61 ctttggactt gtttccttta gccgatattt tcacgatttc tattcatcgg attattgcgc
      121 tctaaccccg gaaagccttt atttcaagtc ttttctacac ccttacttat ctacctttga
      181 catcaatact accgtctcaa cgtggctcgt ctgcggaaac atatcgatgg gctgaatctc
      241 tttgacttga tatacggaat cttcaaataa agccagatcc cgggccaggg tggcagggtt
      301 gcaggaaaca tagatgaggc ttcgggggga tttatttatt acttcctgga ttaattcttt
      361 tttgcagccg ctgcggggcg gatcgaggat gatgacatcg aaatcatctg ttaaagccgg
      421 gacaatctct tcacaggctc ctttgataaa ctgggtgttg gtgatgttat tgagaaaagc
      481 attgcgcttg gcatccttga tggctgcctt gaaattttcc acgcccacta ccctctttac
      541 ttcccgagcc agggacagcg caatactgcc tgtgccgcaa taggcgtcaa gtagcgaatc
      601 actgcgctgc aagcgggcat aatcttttat aacagccaac attttttcat tttgctgatg
      661 attgacttga aagaaggcca gcggggatat ctcaaacttg aattctccaa gtttttcttc
      721 gagccttgtt tccccataat gcaggcgggt gatgttttga tcaatactgt aaaaagatat
      781 ttctttacaa tgatctgcta gaagggcaaa gtcaatttgg ctggtctgat tgccggaaaa
      841 tatgatcatt atttgaccaa gtgaggattg gcgtacggta atctcacatt tatccggcac
      901 gcgcagtatc ttccggtggt tttttatata aaggctgacc gcctgcattg cttggctgat
      961 cagcgggcaa ttctcgatat cgaccatttc atggctttgg ttcataaagt atccgaacat
     1021 ttctctgcca gattgcgaac taagatgcca ctccactttg ttgcggtaat gccaaggggt
     1081 ttccatgcca atgacaggac tgacgggaat ttcaattccg cctacgcgcc gcagggtttc
     1141 ctccacgatt tgtcttttca aagttaattg gcgcggataa tcgacgtgct gaaaagaaca
     1201 gccgccacaa gtaaaataat ggcggcagtt tggttggcag cgatcctgcg aagggtgaat
     1261 tacttgctgc aaaagggcac gctgatagct tttcttttct tcaatgattg ttacggcaac
     1321 ggtttcacca ggaattgcat aagggataaa ggttgccttg ccggcaattc tggcaacgcc
     1381 ttcaccggca tggctgatcc cctccaccaa acactgcatt gactgacctc ctgactggat
     1441 atgcaatcta ctgtttgact tggtcgacaa gcgcctgcaa cagtcgcgct attgcgttgc
     1501 gggtatactc gtcggtcagc ttgccttcgg catcaaattt ttcatgggct ttcatgataa
     1561 atacttccgg tttattaaaa agctgaagat tcagcaccac acagacctgg cgcagatggt
     1621 attgggcacg ggctccgcca aacataccgg gggatgcact catgattgct gccaacttgc
     1681 cgtttaacgg attatcattg cctctcgaag cccaatcgag cgcatttttt aagactggag
     1741 gaatggaata gttatactcc ggggtcgcca gcaaaagagc atctgctgcc gctatttttt
     1801 gtctcaagtc agccaccgca gcgggcattc cttctgcttc cacatcctca ttaaaaaacg
     1861 gaatcattga cagatcagcg atttccagcg cggccccaga cggcagcaat tccttggcag
     1921 cccgtaaagc ggctgtgttg tatgattgtt tacgcagact tccgctcaat cccaaaattt
     1981 taatttcgtt gctcattgcc cctgcctcct tctatattct tttattgatt atcatgccaa
     2041 agttgcctgg cggttatgct cctgcagcca agcctttccg gaaagcgctg aggttgacct
     2101 gtaaagcttt ggcgggaacg atggcgctga tggctttttc cacttcttct gccggaatac
     2161 cgatggctgc agtcagcatg cctaccagta cgacattggc agccttgaca ttgccacagt
     2221 cagccgcaat ggcagttgca tcaatgatgg cagtcttggg cagcaaacct gcaattttgt
     2281 catggatggt atccggatac ttttctttgc cgctcatcac cggcagcgga ttcactcgtt
     2341 cattgttgat gatgaccatt ccgtcgggtt ttagataatc cagccagcgg gcggcttcta
     2401 tcttttcaaa agccaggatg atatcggcat cccctttttt aataatagga gaataaactt
     2461 tcttgccgta gcgcacctgg gaaacaacgc ttccgccgcg ttgcgccatg ccatgaattt
     2521 cggatacttt tacgtcaaac cccatctgta ctgccacctg agcaatgatg cgactggtga
     2581 gcagggttcc ctgtccgcct acgccaacaa tgaaaatgtt tgtcacttta tcctgcatct
     2641 gcttacacct ccatccactt gatcgcatcg aatttacata cttgttcgca cagcccacag
     2701 gcaatacacc cgtcattaat ctgggcgact ttttcctcat ccaggaaggt caccccggtg
     2761 cagccaaccc gtatacatga tttgcagcca gtgcaaagtt cggcatcaac gtaaattttg
     2821 tgatctggtt tgggggttcc gggtaacaat acacagggtc gcttgcaaat aatcaccgaa
     2881 ggttcccgtc gggccagttc ttctttcaag gctagttcca gttcgtcgat tttaagggga
     2941 tcaaccacgc gcaggttttt aacaccgatg gcctttacca gtgcttcaat atccagttta
     3001 ttggtgggct gtttggtaat cgtcaccccg gtagcgggat gatcctgatg cccggtcatg
     3061 gcggtggtat cattgtccag gattaaaacg gtggaagtac cgccgttgta taccacatcg
     3121 ataagtccgg taataccgga atgcacaaag gttgagtcgc cgatcacggc tacggttttg
     3181 gcagcgaatt ccggcccgcg ggctttttcc attcccatcg cggtgccaat gctggcgccc
     3241 atgcagatgc aggtatccat gccttccagc ggggcaatcg ctcccagcgt atagcaccca
     3301 atgtcgccgg ccaccaccaa tcgcatttta tgcagtacat gaaatacgcc gcggtgcgga
     3361 catccggcac agagcagcgg cggacgcgcg ggcacttgct tgtccagatt aaacccttcc
     3421 ggcatgggaa cgccgcccag ctgctgctgc agatattcgg cactgtattc gcccagcaaa
     3481 gtcagctgct ctttgccact cacctcaatt ccccaggatt tcacctgttc ttcgatcact
     3541 ggttcgagtt cttccacgac caccagtttg tcaacctgac tggcaaatga cttaatgagt
     3601 tcctggggca gcgggttgac cagacccagt ttcaaaacgg aagcctgcgg aaacgcttct
     3661 ttcacatatt gataaggtac gccgctggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnng tgtcaaattg ctcgctgatt tcaaaggcca gtttggtaaa gtcgagtgct
     3841 tcctggctgt cggatggttc aatcaccggc attttggcaa atctgccgta accgcggttg
     3901 tcctgctcgt tctgggaaga atgcagcccc ggatcatccg ccgaaacgat taccagaccg
     3961 ccgttgacac cggtatagga gaatgaataa agtgggtcgg cagcaacgtt gatgccgaca
     4021 tgtttcatgg cgaccaaagt tcgagctccg gctatcgagg ctcctgcgcc gacttccaat
     4081 gctacttttt cattgggtgc ccattccgca tatacatccg gaaattcagc aaaactgctc
     4141 acaatctccg tactgggggt acccggataa gctgtggcaa ccgttgctcc tgcttcatag
     4201 gcgccacggg caatggccgc attgcctatc atcaattctt tcatattcat cctcctcgtc
     4261 tccattcgta caataaaaat cctctctttc acaccggaat gagaggagaa actgctgaat
     4321 ttacaaagat aataacataa accaccaaca agtagcaacg taaaatcgct actgcggata
     4381 cagatcatac cagtcggcta cggcttgctg gacaattttg gccatatgat aaacagcagc
     4441 aaaacctggc gaattacccc tcccggcagc agctttatgt cctctttttt ccaaaatggc
     4501 ggtcagggca taatctccaa ccgccggcag tgatttggcg gttgccttgc ccggatagat
     4561 gggtccgttt ctcagctgca atcggccaat ctcatgctcc tcccccaccg ccgcatcaat
     4621 ggcaatatac ttttgccggg gatgacggtt tttaatgatg atcatttccc gggtcagatt
     4681 gcgggcatgc aatggctggt cgagtgtacc gtagacattc agttggggca gtctctcctg
     4741 cagcagggtg ccggtcaacg ggccgaaaca atccaataaa tgtcgatccg agccgataca
     4801 caaaaacacc ggaagtttgg tctcgcctgc ttgacagatg gtcagggcat caaccagcaa
     4861 gcggcgggtt aaagaatcac gataatcggt gtcaacccag atcattgctt taacttcctc
     4921 aaaacttgtt ccagaccgtt tatcagacgc tggttgtctt ccggtcggcc caccgtaatc
     4981 cgaatagcat gcggatagcc aaaatgatca catgttcgta caatgatgcc tctttgcagc
     5041 agttcccgga aaacttcctg gcaatcataa ccgcaatcca ccaggataaa attggcctgg
     5101 gtcgttacgt attccagtcc gatccgtgta aactcacggt atagatactg ttttccctcc
     5161 ttattgagct gacggctttt ggctacatgt gctttgtcag ccagggcagc caaagctcct
     5221 gcctgggcca gtccattgac attgaacggt tccattactg tttcgatcgc ctgggcaatc
     5281 gatgccgtag tcatggcata gccaacccgc agagaagcca gtccatatat tttggaatat
     5341 gtccgactga cgataacatt gcggccctgg tgcacatagg caagactgtc agggaaatct
     5401 ggactttcaa catactcaac ataggcttca tcaaacacaa tgattatatc gtccggtacc
     5461 tgctccataa actgacgggt tgcctgctca ccaagcattg tgccggaagg attgttgggg
     5521 ttgcagataa atatcatttt ggttttcggg gtgaccgctc gcaaaacgcc ttgcaggtcc
     5581 agggtgtaat cttgaagtgg tattttgatg cactgtgccc ccataatcaa tgatacaaat
     5641 tcatattccg aaaacgtagg ctgggcgaag atgacttcat catccttgca gataaaagct
     5701 tccgcaataa gcttcaataa ttcatcagag ccgttgccga taatgatttg gttggcaggg
     5761 atttgataaa aagcagccaa accccgtttt aattcataac aattgccatc cggatagagg
     5821 tagatgtctg gcaatgcctt tgttatggct tgcatcgcca gcggtgaggg gcctaaaggg
     5881 ttttcattgg aagccatttt gatgatgttg tcaatgccca gttctctttt tacctcttca
     5941 atcggtttcc ctgggatata tggtttaagc tgaaagattt cctgccgggc ttttcttttc
     6001 acaaaatcct gcataatcat ttcactcctg atgcaaagat cggttcgccg gtatctgata
     6061 gcagcttttc tatgtacggc ggcagaacaa atgcagcttg atgaatagcg cagctgtagt
     6121 atttcagatc attggcgttc ggtttattaa atctgctgct gcggggatcg tactttttgg
     6181 aacccacagt aaaggtccat ggaccgctga tataggtggg aactgtacta tggtagatat
     6241 atgcctgacc aaaaaccaaa gtaatgttcc ggtaaatctc cctaaagatg tcttcgtaaa
     6301 acagcggcga ttcggactgg gttaccatta aaccgtctct ttccaaggct tggtaaatcg
     6361 tctgatagaa cgcttccgtg aacaagccca cggctggacc agtaggatca gacgaatcga
     6421 cgatgaccaa atcatatttc tggtccgtct ttttgagata gtcaattcca tcccggataa
     6481 tgatatgggt ccgtggatca gaaaaaccag cctgaaaaca agggaaatat tccttgcagg
     6541 tggcaatcac tgcacgatcg atttcaacca aatcgatgtg ctctaccgtt ggatgtttga
     6601 taatttgcgt caatgtacca ccgtcgccgc ctccgattac aaggactttt ttaacctgcg
     6661 gatgggcatg cagggcaacc tgggtaatca tttcatggta catgaactca tccttttccg
     6721 ttgtctgtac cactccgtcc aacaacaaca ttctccccca ttgttctgaa tcggctaccg
     6781 tgatctcttg aaactctgat ttttcttgat gaagaatgtg gtcgatcttc catccaacct
     6841 gatagttttc tgtgtgtttt tcataaatcc atgaaccgtt caattgtttg tcctcctgat
     6901 taattagtca ttctcaccat catactgatc attaaatagg tcagcaatca gtccgcaggt
     6961 atgtttgacc gccgccggat cgttgaaatc cagaggcagc agactggcct taatgatcgc
     7021 gttgatatca tcactccagt tgccttcctg ccattgctgc aggcagattg ccaattcatg
     7081 cggcatctcg ccgtgtgcaa ttttatccag caaggaaagc aggaaataca tctcttcctg
     7141 acttaataaa gcttgctgaa aatgcaatcg cgagatcatg gccaaggtgg catcatatac
     7201 ttttttctgg tttttatcca tgccgttcac taaattgata ccctttttac atccatcatg
     7261 gtctgtcgtt tcaaatcaaa ataaatggga attctgattc cgctttgtcc atcccggttt
     7321 ttatcaatat aaattgcata attgggcatc atgtcatact ttttcagttc gtcactctct
     7381 tccatttctt ccttgctgat gggggtcagt tttaacatca ggtcacattc gtttttcatg
     7441 cgtttggccc cctgtaatgt tccgtccggg ttcaattgca ccagacacat caccacgatt
     7501 ttcaggtttt gggccaacat cttctgggtt ttaacaattt gttcaagaat ctgccattcc
     7561 tgtagtttgg gatcctgctt ttccatgcgt cccacatagt cgatgatcat aatgtcaatg
     7621 tgtttctggg tcttgaactt gcgggcgata gagatggttt tttccggggt gagattagga
     7681 cagggatagg aataaaaacc actttcgccc aaacgcgcat aagcatgcag gatgcgcgac
     7741 aattccgatt cattgatatc accgctgcgg accttgtcgt gttcgatccc agacagtatc
     7801 gagccccagc gcagcgcaat ctgttcccgg ctcatctccg tattcaggta gatgacattg
     7861 tgacgctctt gtacagcgat agcggcggcg gtatgtaacg caaaggaagt tttgccgtgt
     7921 ccggtctgcg cccccagaat gatcaaatct cccggcttgt agcccagggt cactttattt
     7981 aaattgtcaa aaccagtggg caggccatcc agagggataa cccctttgtg cttttctttg
     8041 atttctttaa agcgcaggaa acgtcgttcc acttcctcat agcccaggcg ggcaagatcg
     8101 gccgggttgt caatacgatc gtcgatttcc agggcagtca ggttggtcag ttcttcttcc
     8161 gccagcatca gggtctgttc cacgtcctga tcattctctt cctgcaacaa ctgatagttc
     8221 ttgcgcaaaa acatttcaaa ccggcgcaag cgggatttgt cttttactcg tttgatccaa
     8281 taaatgatat tttcgtcatc gatgtagtgc tcggctatat agctcaactc gtccatatcc
     8341 cgttgattgg caaacactcc caaactgtgg ccttctttga tcagttccac ataggtggga
     8401 tgaacttctc tttcgtataa actggtggtt aactcaaata cagtcgcatg caaaggtgaa
     8461 aaaaaatcgg acgctttcag gctattgtaa gcttctatgc aggctgattc ggcatgcagc
     8521 atggccgaca aaactcttct ttcgctctcg agatcaaaca atgactggtt gtactgactg
     8581 gattccaaga ttgccacctg cctccttgtt taaattgcct tattatgtat tataccttta
     8641 acgtgggaag aaaacagccg gggattctcc cggccgattg tctacgcttt gcttttcagt
     8701 tcctccaaca cctcgggtgg aacgtatatt tcataatcct gggaagaatt catgcttgat
     8761 gctttgccgc cattgagcaa catggatttg gcagtgcttt tattgcgttt tttaaagtca
     8821 acaatctctt tctcagcttc gtcaacggaa cgtatctcct tgctcagcca attgtgcaga
     8881 acgctgtcaa tgtattccaa tcccccgttt ttcgtgcgtt ggcacatgat ctcgccggcc
     8941 ttcatgatca ttggatgaga gaaacgccaa tcatagcgcc acttgtaata catctttttg
     9001 cgagcttccg cgctcatatc aatcccggta aacgtcccaa acgtggcaat atccggatca
     9061 aattgactgg tatgcaggtt gcttcggttg gaatccacat atttgccgaa ataactttcc
     9121 aaaccttcca ccgtattcac ggagtagttc ttcagatctt tggcgatttt ggtgatgtcc
     9181 cggggattat ggatactgcg ttcgaatgtc atttccagca tgcacagcag aaattcaggc
     9241 gtaaagccaa gatcatcaag ccatttctgc aattcatttt ccattttcac actcagcagg
     9301 ttgccgcttt ttttggcaat aaaatcagcc acaattctgt aattggcaga ttcctgggcg
     9361 gattccccgt gatttatgcc ggcgtcggta tgtttgcttt tttcttcctg gagctgcagt
     9421 tccagctgaa caattttttg ttggtatttt tccaatgccg ccgagccaat ctcttcctgg
     9481 ttgctgatat gtaaaggatg gtcacgaatg atcaactgtt gcagtcgttc catcaagggc
     9541 tcgagaaaaa tggttttgtc ggcaaaactt ttgctctttc cgtcctggat ttcaatgaga
     9601 ccaagtttgc tcagttgggt gattttgcgt gaaatctttt gcgtgttcaa gatgggacaa
     9661 caaaccatca attgtccaat ctgaaggcct ttttgaaaca agggtttact ttgctcataa
     9721 gcatagaata aagcggcgag gaatccgata tcttccaagt tcaattcaag ttctttgcca
     9781 taataaaaaa ccgctccggg caaatgcgca aacccccagc ggtatacttc aagtgcggca
     9841 taatcatttg acatatgtac cccagccccc aaacataaag taagaaaatt atatcacttg
     9901 gttgatgatt aacaagtatg attttcataa gcgaaaacct gaaatattta tcttgtacac
     9961 aaaaacgagc gctgagatgc cctcagagta aaaacgaagt ttgacgttaa aaaagagcga
    10021 cagaaatact ctctatcgct ccgcaaagct ataattgaga ggttattttt tccattttat
    10081 cgatcagttc ttcatatttt tgcccggtaa tgctgtcttg atagaaacga ttcagcttca
    10141 ttcttaactg caaatataat tcttcggctt ccagtgaccg attggtgatt cttgtctcag
    10201 tctgcatagg cactgcttta ggttcgatat gcatactctc atccaacgga tattcatcca
    10261 accattgtgg aataaccacc tgtaaatgaa tatcccggcg aattaactcc gccaaagtct
    10321 gttgcgcctt ttcttctccg tggaccagaa agaccgtctt gggcgtcccg tcatatcgtt
    10381 tcagccaact gagcaaacct gattgatcgg catgggaaga ataggcctca atctgcctga
    10441 tgtctgcctt aacagttacc tgttcaccaa agatggtgac aattttttct ccgtccaaaa
    10501 ttttacggcc caaggttcct tcagcctgat atcccacaaa cagaatggtt gattccggtc
    10561 gccagagatt atatttaaga tgatgtttaa tcctgccggc ttcgcacatg ccgctggcag
    10621 aaataataat tgaccggttg atgttgttta gttccatgga ttcttcctgg gaatgggaat
    10681 atttcatatg cggcaattcc aggggatgga agccattatt gacaaaattt ttagtttcat
    10741 tgtcaaacaa ttgcgtatac tttttaaaaa tctcggtggc agcaatcgcc agcggactat
    10801 caatatagac atcaatggat tcgtccaaat cgccttttac atagagttcg ttgagatcat
    10861 atagcagatc ctgggtacgt tccaccgcaa aggcggggat gattagattc ccaccttttt
    10921 tcatggtctc atcaataacc tgtttgagtt tttctacccg gttcagaaca tctttgtgaa
    10981 agcgatcgcc ataggtggat tccatgacta catagtcagc gctttcaata aaggtggggt
    11041 ccttgataaa aggctggttg gtttgcccga gatcgccggt aaaaactatc ttgctgcgtt
    11101 ttcctgcttc ctccagccat agttccacaa tggaagagcc cagaatatgc cctgcatcac
    11161 gcaggcgcac ttcaactcct tctgccggtg tgaacaattc atcataattc accggtcgga
    11221 actgggatag acatttcatg gcatcttcca ctgtgtaaat gggttcgagc ggaatttttc
    11281 cggcccgaag cagtttgcgg tttttgcgtt ccacttctgt ttcctgaata tgggcagagt
    11341 ccggcagcat gactttggcc agttcagccg ttgcaaagct gcaatagatg ttgcctttaa
    11401 atcctaattt gcacaatttc ggaacgagac caatatggtc gatatgggaa tgggataaaa
    11461 tcacaaaatc gagtgtttgc ggattgacct tgaaatcctg atagtttctt tccttgattt
    11521 ccttgcttcc ctgaaacaaa ccgcaatcca tagcaaaaca aagccccccg cattcaataa
    11581 catgcatgga acctgttact gtacgggcgg cacccagaaa ttttatcttc ataaatgatt
    11641 gcctccagtt atatatattg ctcttattat accgcaacaa tctttctggc ggactttttt
    11701 ttcgcaaatt tgttgaagct ttcgcttaat tcaatcactt caaacagaat tgcactgcga
    11761 ttattggccc aacatgcctg cagaatattg tcaacgctgt tgatcccaaa gtccttcact
    11821 atttcgcagc tatccaaatc atctaccaga tccgcatatt tgctgtgaat ctgttcaaaa
    11881 atggcaaagt agatctcttt gaactgcagg gctcgctttt cgatttggtt tttgtcaaaa
    11941 tcctcacgct ctgagatatg cagcagacta tgcaatagac tatgcaagag ctggttgtta
    12001 aaaatggacg caatcaattc ctgtttgtaa tgttttccca gcaagtagcg catataatcc
    12061 cgcaattcct ttctggttgc gtgtccaagc aggctgtatg cattcaggag ttcgacttcc
    12121 tcggaagaca gatcgccaaa catagcaaaa cctcctttgc ttattaataa aggaggttct
    12181 cgctgaccag ttcaaatcct ttagttattt atcgtaatct tcttggtaaa ttacatagtt
    12241 ttcgattaaa tttcaaagat atttgcatgg aaatggcaat tatcctttcc tatgtcgaat
    12301 tacaaaagat tgcattttct gcagtgcggc ttcaatattt ttaacactgg cggcataaca
    12361 gcagcgaata tgcccctccc cgctttcgcc gaaagcagtg ccgggaacaa cagccacctt
    12421 ttcctcaaac aacagttttt cactgaagtc ctcactgttc atgccggtac tggtaataga
    12481 cggaaaacaa taaaaggccc cttgcggttc aaaacaagac agccctattt cattcaagcc
    12541 attaaacatc agtttgcggc gataatcata atccgccacc attttgcgca tctctgtttc
    12601 gccgtggcgc agtgcttcga tggccgcttt ctgcgccata ataggcgcgc acatgatcgt
    12661 atactgatga attttggtca tggcggcaat aatatcctgg tgcccgcagg cgtatccaat
    12721 tctccagccg gtcatggcaa atgccttgga aaaaccgttc ataacaatgg tccgttcata
    12781 catgcccggt aaggcagcga tggacacatg acgggtatgg tatgacaatt cagcgtaaat
    12841 ttcatcggaa ataacaatca aatcgtgttt gataataatt tcccgcaatg cttccaggtc
    12901 ttgtttttcc attatcgcgc cggtcggatt gttgggatag gccagaatca aaaccttggt
    12961 gcggggagta atttttgctt ccagttcctc tggttgcaga cggtattgat gctgcgcata
    13021 acaagccagt ggaaccgggg tcccgtaagc cagcaaaacg ccaggcgcat aactgacata
    13081 acaaggctcc ggaatcaaaa cctcatcccc cggttccacc agggaacgta aggcaatgtc
    13141 gatggcttca ctgactccaa ccgtgaccag gatctgattc tggggtgagt aatccacgcc
    13201 aatggctcgc ttttcatatt gggcaatctc ttcccgcaat tccggcaggc cccaattaga
    13261 ggtatacatg gtataaccgc tttccagcga atacaaacaa gcctcacgaa tatgccaggg
    13321 ggtaacgaaa tcaggttccc caacccccag ggaaatcacg ccctcggtct gggagaccag
    13381 gtcgaaaaat ttacggatgc cggaaggctt cagagattga acggtatcgg cgatatggtg
    13441 ctgcatgctg ctcatggact taccaccagc cttttatctt cttcattttc accaaatata
    13501 atatggtctt ccttgtaacg tttgagtata aagtgactga cggtactttg tactttgtcc
    13561 agcggtgaca gcttgtgggt aacaaaatgg gctacatcct tcagactgtt gccttccacg
    13621 atgaccgtca aatcaaaacc tccgctcatc agatagaccg aacgcacttc agggaaacgg
    13681 taaatccttt cggccacact gttgaagccg acttcccgtt cggggctgac cttgacttca
    13741 atcatggccg taaccccttc ccattcagtg ttctcccagt tgacaacggt acggtacccg
    13801 agaatcgttt tgtccctttc cagctgcgcg atgatatcgg caaccacctt ttcatctgta
    13861 tccaacatcg ctgcgattgt cttggcgctt aatcgggcat cgttttccag taattccagt
    13921 attcttctct cgatatccat gacattccct ccatttcaat cataaataaa aaaacaaaac
    13981 tctcctccct gaatagggac gagagttatt tcccgcggta ccaccctgct tggcaaatgc
    14041 ccggctttta tccaataacg gttggattcc gtccggctta tccactcggt tcaccggcac
    14101 tcacagatgg ccttcattgt tgttttgata ccggtttgca gcggccccgg ctctctgttg
    14161 atcaaaacgc gcaatgattc ttcttttcat cattttcgca tattagatta ggatgtattt
    14221 tatcacccct gatatagcga gtcaagcggt attttttctg ataataatcc aaaatttccc
    14281 gaactaaaaa tcctgcccgg agcatagaat tcctttaagg gatttcggga tggtgattac
    14341 atgataaaaa cggatcaatt aaatcaggaa gaaagaattg tttttgaacg catcaatgaa
    14401 tactttaaat ctcctgccat gaccgcgcag gagaaaatca tcaatgctct gctgattgcc
    14461 cagctggaat tggatgatca tcatttctgc aatgaaaaag aacgattaaa aatcatccat
    14521 tttcaaaaga cattggacag tctgctcggc aaaatagtga atcagtcttg actcgnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaggc cccaacacca agatttataa
    14701 gcttttcaaa catatccaca accatacctc ccatcacgaa ttcatctgtt cttcctgtct
    14761 atgccaatag aataacacaa aacagaccgc ctttatagtt atcatttcca ttcccttttt
    14821 acttcataac cgatcttatc aaaagttcga atatttatta tattatgata tttttttctt
    14881 ttaattgtcg acttaataaa atatgataat cattaaggga ggtcgtaaca gatgaaaaac
    14941 ttgcttgcca ataaatccta caaatttatc tttgaacact ccagtgatgc gattctctta
    15001 accaaaaccg acggcagcat tcatctcgcc aatcctgccg cttgcagtat gctgcacaga
    15061 acagcgcaag agatttgcca aatcggacgc aatggagtcg tagatttgca ggatccccgc
    15121 ctggtaaccg ccctgcagga aagagacagc cacggcaatg tgcggtgtga attaaatttt
    15181 gtacgggcag acggggtaaa aataccggtg gaaattacct caaacgtttt ggccgacaaa
    15241 gaggatagtc attggaacgt aatgataatc cgtgacatga ctgtgtacaa ggaactggaa
    15301 gaaagctata aaaggagtca tgaacaagcc ctgcagatgt gtgtgctgga ctttttaacc
    15361 ggcatctata atcggcgggg ttttatggag cggctgcagc aggaactcga acgcagtaaa
    15421 cgggatggtt tgcaaataag tatcatcatt gcagatttgg ataatttcaa gcaggtcaat
    15481 gatgtctatg gacatttggc cggtgattat gtattaaaac agtttgctga ctgcctgaat
    15541 cagaatgtac ggccctatga tatattggct cgatttggcg gggatgagtt tattattggt
    15601 ctgccaggca cgggacttct tgaagcaacc aacgttgctg aaagactgcg cagcatttct
    15661 gaaaaccttg ttattaccgt cgattttcag gccattgatt tgaccgcctg ttttggtgta
    15721 actgcctatc gaaaagatgt tcaggatgat ctggatacac ttatctcccg ggccgatgat
    15781 gccatgtata aagccaaaaa gactaaaaat gctgtgtttg caaaagaata atacggcact
    15841 taaaaacttc atctaaatga aataataggg gttttataac ccctattaca cattttataa
    15901 ttctggtggg ccatgaagga atcgaacctt caaccttctg attaagagtc agctgctc
//
LOCUS       DAOTHZ010000050        15950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4428_length_15950_cov_6.626109, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000050 DAOTHZ010000000
VERSION     DAOTHZ010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 15950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15950
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4428_length_15950_cov_6.626109"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="P5549_06990"
     CDS             <1..1119
                     /locus_tag="P5549_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12853.1"
                     /translation="MGKGTYCLPQYAKSFRHNALFSGKPSRQAIREGRADYTSIYLHE
                     LPILFTSGQFPIDVAMVTVSPPDEHGRISLGISVDYTLQAIKSAKAVIAEVNPHMPVT
                     GEGSLIPVEDVDYFVPVTYPLITQNPPVIGEVERTIGQNIADLIKDGDCLQLGIGAVP
                     DAILSCLNDKRDLGIHSEMISDGVMNLVKSGVINCSRKNYYPNKIVIAFAMGTSEFYQ
                     WLDHNPLIEALPVSITNEPYNICKNDNMVAINSALSVDLLGQVAADTLPGIQFSGVGG
                     QVDFVRGANNSKGGRAIIALPATAAGGSVSRIVATLAPGQAVTTSRYDVDIVVTEYGV
                     AHLKWKTNRERAQALINIAAPEFRDELRAEYIRLYGSL"
     gene            1346..3139
                     /locus_tag="P5549_06995"
     CDS             1346..3139
                     /locus_tag="P5549_06995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12854.1"
                     /translation="MKQEKVLLEKIFNEIPHALLLLDDDDRVATSNAAAARLLALPKD
                     ARGRKIEEVIHYKAVLKLVQNDCKEGKQNIKIKDKKYICRSTCLETDDFKGKLILLLD
                     QTALESISNELAETRVLKEEMDKIIDSSFDYIFVTDDQGNVRKINDAYTRITGFKAEE
                     IVGRNIYDLVKKGYFDRAATIDVIETRKTVTLTQTLKSGKTVLVTGNPIFSESGEFIG
                     VVTNGRDITELNRLKSEVRKAETLSRHYQNELLKFQLDNTGDYIVASPQMAEISDLVQ
                     RIARVDSTVLINGESGVGKEIVAREIHRNSLRADQPYISVNCAAIPDNLLESELFGYE
                     AGAFTGASKSGKMGIFQLADSGTLFLDEIAELPFYLQAKLLRVIQENEIMRIGAVAPI
                     PINVRLITATNRDLWKMVSERQFREDLYYRLNVVQVRIPPLRERKEEIPVFVDYFIGL
                     LNRKYKLNRQFNPELLAGMMAYDWPGNIRELRNALEQAFVTTSGAFINDIKMGPQQEV
                     HLSAEAVQPQEIKPPGRKPMPPGYDEASESDRVSLKDALQKYESQIIKEAMEAHQTTR
                     RAAAALGVSQATIWRKAKQYDITIEEENLNT"
     gene            3385..4167
                     /locus_tag="P5549_07000"
     CDS             3385..4167
                     /locus_tag="P5549_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRW12855.1"
                     /translation="MPYDNILLENRQQVQLLYINRPKVLNALNEKTLLEIEAAVQAFA
                     ADEARAVLIITGSGERAFVAGADIEAQCPLDATQGRNWGLLGHRVFRLIETTEKPIIA
                     AINGFALGGGCELAMACDIRLASEKAQLGQPEVSLGITPGYGGTQRLTRLVGEGKAKQ
                     LVFTGQRIPAAEAYRIGLVDEVYPPESLLEEAVKMAEVIAANAPLAVRYAKQQINHSM
                     QINNDLATTVEAGLFGLCFATRDQKEGMGAFLEKRKPVFEGK"
     gene            4193..5338
                     /locus_tag="P5549_07005"
     CDS             4193..5338
                     /locus_tag="P5549_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12856.1"
                     /translation="MEFSFSSDQEMLRQSVRVFAENVLAPGVVERDEKEYYDPNTFKA
                     MADLGLTGIPFEEKWGGADMGMVAYAIAVEEIARIDPSAADTLSAHVSLCAFPIAMFG
                     HALQKEKYLPALATGEKHGAFALTEPGAGSDALGMTTKAEFKNKQYILNGRKIFITNG
                     AAADIYIVFARTGAKEEGSRAFSAFIVEKGTPGFSFGKFERKMGLRGTQNCDLVFEDC
                     RIPAENLLGAEGQGFKIAMQTLDGGRIGIAAQAVGIAQGAMEKALEYAKTRVQFGQPI
                     VNQQAIAFKLADMALEIDAARLLTYRAAWNKSQGLPYSLQAAMAKLKASETANWVTNQ
                     AVQIHGGYGYTREYPVERMMRDAKITELYEGTSEIQRVVISNNLIHR"
     gene            5425..6300
                     /locus_tag="P5549_07010"
     CDS             5425..6300
                     /locus_tag="P5549_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW12857.1"
                     /translation="MEMLLQIIASGLLLGGIYALLSVGLNLIFGVIKIVNLAHGDFLM
                     MGMYATFFLFTLKDVNPYVSVLIVFPAFVLIGVVVFRVIIKPLLGKAETEVNTIIATA
                     GVGFVLQNLALMLWKSDFRAIETNLTGKSYEFFGVLLSAGRTWTFVICIIVALLLYYI
                     LMKTKVGTHIRAVSQDTDAATLMGINVNRIYTLTFAAGIALVGIAGSIITPTFYVYPT
                     VGLYFNTMAFIIVVLGGLGSFTGALIGGLLIGLVESFAGVMASAELAQVFSLLIFLII
                     LFVKPEGIMGKGARV"
     gene            6319..7320
                     /locus_tag="P5549_07015"
     CDS             6319..7320
                     /locus_tag="P5549_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW12858.1"
                     /translation="MKQKQSLIVLGILGVALLALYFLPLIVFAKHILILTLFYAFLGT
                     GWNLMCGYAGRLSLGHAAFVAVGAYTSILLFKEFSLTPWLGMLVGGVLAVVVMLLIAW
                     PSFRFGLKGPYFTLASIAVAEIVHNLLVYGRDITGGSLGLSLPYVPTSLAQFQFDGKG
                     EYYLVILVFWLAAMFLTIKMERTRYYLEAIREDEDAASALGISVNRNLIISACISAFL
                     VAIGGTFYAQYFRYIDPYSIAGFSLSLNMALVVVIGGSGTIFGPTIGAFLLIPMTELL
                     RMSLGAQLPGLHLFLYGILLVACVIYLPKGIASLPGIIKAKREKKALQIQQTSEGVQ"
     gene            7321..8052
                     /locus_tag="P5549_07020"
     CDS             7321..8052
                     /locus_tag="P5549_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12859.1"
                     /translation="MAETILVIDGISKSFGGLQATDNVSFDVNKGEILGIIGPNGAGK
                     STLFNLITGFYPIDSGSITYHGKRIDKLTPDKICHKGITRTFQVAKPFPGLTIADNIL
                     VACLPKAAKVSEARDQVDAILKIVKLDKKANSLGADLTTLERKRLELGKALATNPEVL
                     LLDEVMTGLKPKEMDEMMDLIRELKKTKTIVIVEHVMRVIMNISDRIIVLDHGQKIAE
                     GLPKEIAENEVVIKAYLGRGSNKDA"
     gene            8045..8743
                     /locus_tag="P5549_07025"
     CDS             8045..8743
                     /locus_tag="P5549_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12860.1"
                     /translation="MLKLEKLSVSYGDTQVIWDVDIEVEQGEVVTLLGPNGAGKSTLM
                     KTICGLVAPKSGKISFLGEDLLKINPWDRPKKGVVLIPEGRKLFGKMTVEENLLIAAW
                     KEEGKKETLEWIYETLPRLAERRKQLAGTMSGGEQQMCAIARGIMAQPKLLMLDEPSL
                     GLAPLIVEQVFDLIQKLQQRGITVFFVEQFVEQSLKIASRGYLLEGGRIVLQEDSQSM
                     LNNDYVRQVYLGIA"
     gene            8765..10114
                     /locus_tag="P5549_07030"
     CDS             8765..10114
                     /locus_tag="P5549_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW12861.1"
                     /translation="MKKFVGILMLVVFMMVAVAGCEKAPTEGGEAKDIKIHVGVVEPI
                     SGAQAALGQAEYDGIQMAIEMINEKGGVMGKYPITFDFADSQSDPATGASEAERLITS
                     KGVPVIMGSYSSAIAAAISEVAERNKVVLWEMSGSADNLLQKGYQWTFRNEANASAWG
                     AESVEFIMGNDAQIQEKLGKPAKELVVAIVHEDGPYGTAVAEGNQATAEKYGLKVAMN
                     EAYSAKAVDLSSIIMKIKSSNPDVLLLTSYVNDGIMFNRQAKELGLKVPIFITHSGGH
                     SVQAFVDGVGADANSMLTVDPVPCNPNTAGFSAEYAAIFDDFVKRWTDKFGVPPYHHV
                     EYRQFAQTMLFFNEIVPAAIEKSGEFSAQAVADAIRAADVPAENSLCGFGAKFSTPES
                     PWTDPWLGNKHTGQNYEARAFINQYFDGTLVTVWPENLAKQKAELFLPASHPLSANK"
     gene            complement(10365..11753)
                     /locus_tag="P5549_07035"
     CDS             complement(10365..11753)
                     /locus_tag="P5549_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRW12862.1"
                     /translation="MLLLLRNKPLRATIQRTALPAMLEMILYMLIGIVDVAVVGRLGA
                     VPLAAVSLGAEIFFEVILFLGALGIGAAVLAAQARGAGNVHEAEQIAGQTLLLGLLMG
                     CLSGFLGVYYADFLVGLFRVEPAVHALAVRYVQITFAIAPLGVLYTMLSSLYRGWGRT
                     EIPMYVAIVLNIVNCIGCYVLVYGKFGFPALGVAGSALATAIAHVVGCLIIFTILIRG
                     GGGLKLRWDAIRRLQRQVLKRIFSLGLPSFVEQFFNNLSIMIAVYLIVFTGTVSYAAH
                     QVGVIVESISYMPGFGIAIAATTLVGQSVGARDKERLLQATRGTIEFGLLFMGFFALL
                     FALFPFPIARLFTHDPAIIAIAGLLIRIAALEQLTIALSMVMGGILKGSGDTRTPMVI
                     TVLATWAYRIPMLYLVIIVLAQPIQIAWLVFISDWLLRAIAFYLAYRRRTRPDGPFLS
                     TFEPQESSAPAQ"
     gene            12053..12910
                     /locus_tag="P5549_07040"
     CDS             12053..12910
                     /locus_tag="P5549_07040"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRW12863.1"
                     /translation="MEYQQIKYEVEDRILTITLNRPEQMNTYNYTLHYELIDAFDRAD
                     EDDNIRAIIITGAGKAFCAGMDLSEKGSSFSPGSMPIEEFRDGGGMMALRMFQCKKPL
                     IGAINGPAVGFGFTFTLPMDIRIGSTKAKMGMVFVRRGVLIDACSSWFLSKVLGINRA
                     LEWAMTGKVYSAQELYEGGLFNYLVEPEELIPKAREIAKEIAENTAPVSVAITRQLIW
                     TMAGAEHPMQAHRIESKGFHWAGQQPDVVEGIMAFLEKRKPNYSMSVKDGMPPFYPWM
                     PEPPFKEEK"
     gene            13204..13590
                     /locus_tag="P5549_07045"
     CDS             13204..13590
                     /locus_tag="P5549_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HRW12864.1"
                     /translation="MKPTVLLFAFQDAEKREKMARALLPLKLRIKKINKSDYHQPLGA
                     LLGIAAEEPAIAAYQGPDLPHEMMLIAGCSDPQIHALLDALRNAGIPRIHHKAVLTEN
                     NQYWNALELYEELNQEYEYFRQQEGQ"
     gene            13998..14369
                     /locus_tag="P5549_07050"
     CDS             13998..14369
                     /locus_tag="P5549_07050"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRW12865.1"
                     /translation="MKEKMRSTFDAFADLLDDAVDRIPDRFLRDLTGGFNLRREAKRE
                     DDYFILGEYVEDGLGGCFIVFYYGSFAVLLADEPVTVWEAEIIDTVLHEMQHHLEAMA
                     GRDDLAQQEMEELERALQKGE"
     gene            complement(14484..15557)
                     /locus_tag="P5549_07055"
     CDS             complement(14484..15557)
                     /locus_tag="P5549_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HRW12866.1"
                     /translation="MKIRQTIVLGVIGSLFFIGTVIASPASAESPATMLPAEAVTAIT
                     QDSQITFEVIKEKHDVVEIDITYPVISGLTDADFQEQLNYFIKKQVIYSKDLIEMQAD
                     EFVQEAKKNGWEMRPYVLSIDYDLKSNDEAFLSFTMTYYTFTGGANGMTVVNCFNIDK
                     KANKPLQLAALFPPGADYKAVMNREIAKQIELRSQDDNVMFFEGDMGFKGISDTQGFY
                     LKDNDIVIVFPKYEIAPGAMGIPEFAVNRADLKKSVAAQDGEIVIAGLVFKTVLNAET
                     QTVMIPLRQVGEHLGYTVDWNAAQRSVMLTKGQATAKLKIDDNIYIANHKIPYILEAV
                     PIIMNDRTFVPVSFLEQVLLYQL"
     gene            complement(15754..>15950)
                     /locus_tag="P5549_07060"
     CDS             complement(15754..>15950)
                     /locus_tag="P5549_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12867.1"
                     /translation="GRFLPCFLPPESKARTVPDLRDLRQINAAVCNIRITITTASARV
                     ILRSGSAIPWLPTINFCFMQ"
BASE COUNT         4132 a         4093 c         4112 g         3613 t
ORIGIN      
        1 atgggcaagg gaacctactg cctcccgcag tatgcgaaga gtttccggca taatgccttg
       61 ttttccggca aacccagccg tcaggccatt cgcgagggcc gggctgatta tacctccatc
      121 tatttgcacg aattacccat cctttttacc agcggacagt ttcccatcga tgtcgccatg
      181 gttaccgttt caccgcccga cgagcacgga cggatcagcc tgggcatatc agtggattat
      241 accttgcagg ccataaaaag tgctaaagcc gtaattgccg aggtcaatcc ccatatgccg
      301 gtgaccggag agggttcctt gatcccggtg gaagacgttg attattttgt gccggtaact
      361 tatccgctca ttacacagaa tccgcccgtc atcggcgaag tagagagaac cattggccag
      421 aacattgctg atttgatcaa agacggggat tgtctgcagc tgggcatcgg ggcggtgccg
      481 gatgccatcc tgtcctgcct gaatgataaa agagatctgg gcatccattc ggagatgatt
      541 tccgacggcg tcatgaacct ggtcaaaagc ggcgtgatca actgctcccg caaaaattac
      601 taccccaaca aaattgtgat tgcctttgcc atgggcacca gtgaatttta tcaatggctg
      661 gaccacaatc cgttaatcga ggcactgccg gtcagtatta ccaacgaacc ttacaatatc
      721 tgcaaaaatg acaacatggt ggccataaat tccgccttgt cggttgattt gttggggcag
      781 gtagcggctg acacactgcc cggcattcag ttcagcgggg tcggcggaca ggtggacttt
      841 gtccgcggcg ccaacaattc caaaggtgga agggctatca ttgctttgcc ggcgaccgct
      901 gcaggcggca gtgtttctcg catcgtagcc acgctggccc ccgggcaggc ggttaccacc
      961 agccgttatg acgtggacat cgtagtgacc gaatatgggg ttgcccattt gaaatggaaa
     1021 accaaccggg aacgggccca ggccctaatc aatatcgccg cacccgaatt ccgggatgag
     1081 ctgcgcgctg agtatattcg cctttacggc agcctttaaa ggctttcaat cgtgatagaa
     1141 ttgaatatca cccccctcct ctttctaatc agaccttgcg aaaggtctga tttttttata
     1201 gcggctgttg gcagcgatat tttacaatca gccggttttg atttattatt gagtcaatca
     1261 ctggctgccg gtcaaagcag taacagcaag ggtggccgct gtacgatgta aaagtggaat
     1321 cctggcggaa atggggtgcg cataaatgaa acaggaaaaa gttttattgg agaaaatctt
     1381 taacgaaata ccgcatgcct tgttgctcct ggatgacgat gaccgggtcg cgacctccaa
     1441 tgctgccgca gcgcgtctgc tggctttgcc caaagacgct cgcggccgaa aaatagagga
     1501 agtcattcat tataaagcgg tgttgaagct ggtccagaat gactgcaaag aaggcaagca
     1561 aaacatcaaa atcaaggaca aaaagtatat ttgtcgttcc acctgtttgg aaacggatga
     1621 ttttaaaggc aaattaattc tcttgctgga tcagactgcg ctggaatcga tcagtaatga
     1681 attggcggaa acccgggttc tgaaagaaga aatggataaa atcattgata gttcctttga
     1741 ctacattttt gtcaccgatg accagggtaa tgtcagaaag atcaacgatg cctatacccg
     1801 catcaccggc tttaaggcgg aagagattgt gggacgaaat atctatgacc tggtgaaaaa
     1861 aggatatttt gaccgggcgg ctaccattga tgtaattgaa accagaaaaa ccgttaccct
     1921 gacccagacc ttgaaaagcg gcaaaaccgt tctggtgacc ggcaatccca ttttttccga
     1981 gagcggggaa tttatcgggg tggttaccaa cgggcgcgac atcaccgagc tgaatcggct
     2041 gaaaagtgag gtcagaaaag ccgagaccct gagccggcat taccaaaatg aattgcttaa
     2101 attccagctg gacaataccg gcgactatat cgtggcctcg ccgcaaatgg cagagatatc
     2161 agacttggtg cagcgcatcg cccgggtcga ctctacggta ctcatcaatg gtgaatcagg
     2221 cgtcggcaag gaaattgtag cccgcgaaat ccaccgcaac agcctgcgcg cggaccaacc
     2281 ctacatcagc gtcaactgtg ccgccattcc tgacaatctg ctggagtcag agctattcgg
     2341 ctatgaagca ggggccttta ccggtgccag caaaagcgga aaaatgggca tttttcagct
     2401 ggctgacagc ggcacgctgt tcctggatga gatcgccgaa ctgccatttt atctgcaggc
     2461 caaactgctg cgggtcattc aggaaaatga aatcatgcgc attggcgccg tggcgccgat
     2521 acccattaac gttcgcctca tcaccgccac caaccgcgac ttgtggaaaa tggtaagtga
     2581 acggcagttc cgcgaggatc tgtattaccg gcttaatgtt gtccaggtgc gaatcccgcc
     2641 gctcagggag cgcaaggaag agataccggt ttttgtcgat tactttatcg gtctgctgaa
     2701 tagaaaatac aaattgaacc gtcagttcaa cccggagctg ctcgccggga tgatggcgta
     2761 cgattggccg ggcaatattc gggaactgcg caatgccctg gaacaagctt ttgttaccac
     2821 gtccggagcg tttatcaatg atattaaaat ggggccgcag caggaggttc atctgagtgc
     2881 cgaagcagtc cagccgcaag agataaagcc gccaggcaga aagccaatgc cgccgggtta
     2941 tgatgaagcg tcggaaagcg acagggtttc cttgaaggat gcgctgcaaa agtatgaaag
     3001 ccaaataatc aaggaagcca tggaggcaca tcaaaccacc cgcagagccg cggcggccct
     3061 aggtgtcagc caggcgacca tctggcgcaa agccaaacag tatgacataa ccattgagga
     3121 agaaaacctg aacacgtgaa ttaaatttga aacaaaaccg ggtcgaatga atcaattatg
     3181 tgtcaaaata catcgttaac aatcagccag tcaataaaag actggcagaa tacagccggg
     3241 tttgaaaggg cgcgatacct tcacctgccg gctgtatttt ttattggcgg ttaacctctc
     3301 ccggctcgtc aagcactgga atatttcatg gcataggaat tgcatgattt ttaattacaa
     3361 agtatatttg cgggagggaa gaacatgccc tatgacaata ttctccttga aaaccggcaa
     3421 caagtgcagt tgttgtacat caaccgcccc aaagtcttaa atgccttgaa cgaaaaaaca
     3481 ttgttggaaa tcgaggcagc ggttcaggct tttgccgctg acgaagcacg ggctgtcttg
     3541 attatcaccg gcagcggcga gcgggccttt gtagccggag cggacatcga agcccagtgt
     3601 cccctggacg cgacgcaggg aaggaactgg gggttgttgg gacaccgggt cttcagactg
     3661 attgaaacta ctgagaaacc catcattgca gccattaacg gttttgctct gggcggcggc
     3721 tgcgaactgg ccatggcctg tgacatccgt ctggcctccg agaaggccca attgggtcag
     3781 cccgaagtat ccctcgggat tactcctgga tatggcggca cccagcgtct tacccgcctg
     3841 gtcggcgaag gcaaagccaa acaactggtc tttaccgggc agcgcatccc tgctgccgaa
     3901 gcatatcgta tcggactcgt agacgaagtc tatccccccg aaagcttgct ggaggaagcg
     3961 gtcaaaatgg ctgaagtcat tgccgccaat gcacctctgg cagtacgtta tgccaaacag
     4021 caaatcaatc acagcatgca gatcaacaac gacctggcca ccaccgttga ggccggttta
     4081 tttggcttgt gttttgccac ccgggatcaa aaggaaggga tgggcgcgtt cctggaaaaa
     4141 aggaagcccg tttttgaagg caagtaatcc acagagagga ggaaagccag ccatggaatt
     4201 ttctttcagc agtgatcagg aaatgctgcg ccagtctgtg cgggtttttg ccgaaaacgt
     4261 acttgccccc ggcgtagttg agcgggatga aaaggaatac tatgatccca atacattcaa
     4321 agccatggcc gacctgggat taaccgggat ccctttcgaa gaaaaatggg gcggggctga
     4381 tatggggatg gttgcctatg ccattgccgt tgaagaaatc gcccgtatcg atccttctgc
     4441 cgccgatacc ctgtccgcgc atgtcagcct gtgcgctttt ccgattgcca tgtttggaca
     4501 tgcccttcaa aaagaaaaat atctgcccgc actggcgacc ggagaaaaac acggcgcctt
     4561 tgccctgacc gagccgggag ccggttccga tgcgctgggc atgaccacaa aagccgagtt
     4621 caaaaacaaa cagtatatat tgaacggacg taaaattttc attaccaacg gcgccgccgc
     4681 cgatatctat attgtttttg cccggaccgg ggccaaggaa gagggcagcc gggctttcag
     4741 tgcctttatc gtggaaaaag gcacgcccgg attttcgttt ggcaagttcg aacgcaagat
     4801 gggcctgcgc ggcacccaga attgtgatct ggtgtttgaa gattgccgca ttccggctga
     4861 gaacctgctg ggggcagagg gacagggctt taaaatcgcc atgcaaactc tggatggagg
     4921 ccgcatcggc attgccgccc aagccgtagg gattgcccag ggagccatgg aaaaagcctt
     4981 ggaatatgcc aaaacccggg tacagttcgg acaacccatc gtcaaccaac aagccatcgc
     5041 ctttaaactg gcggatatgg cgctggaaat cgatgccgcc cgcttgctga cctatcgggc
     5101 agcctggaat aaaagccagg gactgccgta ctcgctgcaa gcggccatgg ccaagctgaa
     5161 agcttcagaa acggccaact gggtcaccaa tcaggcggtg cagattcacg gcggttatgg
     5221 ttacacccgg gaatatccgg tggagaggat gatgcgtgat gccaagatta ccgaactgta
     5281 tgaaggcacc agcgaaatcc agcgggtcgt catcagcaac aacctcatcc accgttgagt
     5341 tatcccggcg ttggcaaacg ccatcaatta ctataaatca ataccgttgc ccatactatt
     5401 gaaagaatgg atgtgaaaaa gggaatggaa atgttactgc agattatcgc cagcggtttg
     5461 ttgctggggg gcatttacgc gcttttgagc gtagggctga acctaatctt cggggttatc
     5521 aagatcgtca acctggccca cggtgatttt ctcatgatgg gaatgtatgc cacatttttc
     5581 ctctttacct taaaagatgt gaatccctat gtctcggtct taatcgtatt tcccgctttt
     5641 gtgctgatcg gggtagtggt cttccgggtc attatcaaac ccctgctggg caaggcggaa
     5701 accgaggtca acaccatcat cgcaaccgcc ggtgtcggct ttgtgctgca gaacctggcc
     5761 ctgatgctgt ggaaatcaga tttccgggcc attgaaacca atctgaccgg caaaagctac
     5821 gagtttttcg gtgtcttgct gagcgccgga cggacctgga cttttgtcat ttgtattatc
     5881 gttgccctgc tgctttatta catcctgatg aaaaccaaag tcggcaccca tatccgtgcg
     5941 gtttcccagg ataccgatgc cgctaccctg atgggcatca atgtcaaccg catttacacc
     6001 ctgaccttcg ccgccgggat tgcactggtc ggcatcgccg gcagcatcat cacccccacc
     6061 ttctatgtct atcccacggt cggcctgtat ttcaatacca tggcctttat catcgtggtg
     6121 ttgggaggat tgggcagttt taccggcgct ttgatcggcg gtttgctgat cggcctggtc
     6181 gagtcttttg ccggggttat ggccagtgcc gagctggcgc aggtgttctc cctgttgatc
     6241 ttcctgatca tcctgttcgt gaaaccggaa ggaatcatgg gcaagggtgc cagagtatag
     6301 gaaaaggagg gagaaagaat gaaacaaaag caatcactga tcgttctggg cattttgggc
     6361 gtcgccctgt tggcgctgta ctttttgcct ttgatcgtct tcgccaagca tattttaata
     6421 ctgaccctgt tttatgcttt cctgggtacc ggctggaacc tgatgtgcgg ttatgccgga
     6481 cgactgtcct tgggacatgc tgccttcgtc gccgtcggtg cctataccag tatactgttg
     6541 tttaaggaat tcagtcttac cccttggttg ggcatgctgg tgggcggcgt gttggcggtg
     6601 gtcgtcatgc tcctgattgc ctggcccagt tttcgctttg gtttgaaggg tccctatttc
     6661 accctggcca gtatcgctgt tgcagaaatt gtccacaacc tattggtata cgggcgtgac
     6721 attaccggcg gttcgctggg tttgtccctg ccttacgtgc ccacctccct ggcgcagttt
     6781 cagtttgacg gcaagggaga gtactatttg gtgattttgg tcttctggtt ggccgccatg
     6841 tttctgacca tcaaaatgga acgaacccgc tactatctgg aagccattcg cgaagatgaa
     6901 gatgccgcct ccgcgctggg catctccgtg aacaggaatt taatcatatc cgcctgcatc
     6961 agtgcctttt tggtcgccat cggcggaact ttttacgccc aatatttccg ctacatcgat
     7021 ccctattcca ttgctggatt ctcattgtcc ctgaacatgg ccctggtggt ggtcatcggc
     7081 ggcagcggca cgatttttgg acctaccatc ggcgctttcc tgctgatccc catgaccgag
     7141 ctgctgcgca tgtccctggg ggcacaattg ccgggattgc acctgttcct ctacgggatt
     7201 ctgctggtag cctgtgttat ctatctgccc aaagggattg ccagtctgcc agggatcatc
     7261 aaagccaaaa gagaaaaaaa agcgctccaa atacaacaga cttctgaggg ggtgcagtaa
     7321 atggcagaaa ccattctcgt catcgacggt atatccaaaa gtttcggcgg actgcaagcc
     7381 accgacaacg tatcttttga tgttaataaa ggagaaatcc tcggtattat cggccccaac
     7441 ggtgccggca agtcaaccct gttcaacttg attaccggtt tctatccgat tgacagcggt
     7501 tccatcacct accatggaaa acgcattgac aagctgacgc ctgacaagat ttgtcacaag
     7561 ggcatcaccc gaacctttca ggtggccaaa ccgttccccg ggctgaccat cgccgacaac
     7621 attctggtgg cttgcctgcc caaagcagcc aaggtctcgg aagctagaga ccaggtagac
     7681 gcgattttaa aaatcgttaa gctcgacaaa aaggccaact ccctgggcgc tgacctgacc
     7741 accctggagc gcaaacggct ggagctgggc aaagccctgg ccaccaatcc tgaagttttg
     7801 ctgctggatg aagtcatgac cggtctgaaa cccaaggaaa tggatgaaat gatggacctc
     7861 atccgcgagc tcaaaaaaac caagaccatc gttatcgtcg aacacgtcat gcgggtcatc
     7921 atgaatatca gtgatcgcat catcgtgctc gatcacggac aaaagatcgc cgaaggtttg
     7981 cccaaggaga tcgccgagaa cgaagtggtc atcaaagcgt atctgggaag ggggtcaaac
     8041 aaggatgctt aaattggaga aactcagcgt atcctacggc gatacccagg tcatctggga
     8101 tgttgacatc gaagtcgagc aaggcgaagt tgtcaccttg ctggggccca acggggcagg
     8161 taaaagcacc ctgatgaaaa ccatctgtgg cctggtcgcc ccgaaatccg gcaaaataag
     8221 ctttttgggc gaagacctgc ttaaaatcaa tccctgggac cgccccaaga aaggtgtagt
     8281 actgattcct gagggccgga aactgttcgg taaaatgacc gtggaagaaa acctcctgat
     8341 cgctgcctgg aaagaagaag gcaaaaaaga aaccctggag tggatatatg aaaccttgcc
     8401 ccgtctggcc gagcgccgca aacagctggc cggcaccatg agcggcggcg aacagcaaat
     8461 gtgtgccatc gcccgcggca tcatggctca gcccaaacta ctcatgctgg acgaaccctc
     8521 cttgggtttg gccccgctca tcgtggaaca ggtttttgat ttgatccaaa aattgcagca
     8581 aagaggcatc accgtctttt ttgtcgagca gtttgtagaa caatctttga aaatagcatc
     8641 cagaggatac ttgttggaag gggggcggat tgtactgcaa gaggacagcc aatcgatgct
     8701 gaacaatgac tatgtgcgtc aagtttactt gggcatagcc taaggacaag aggaggtaag
     8761 tgtattgaaa aagtttgttg gaattcttat gttggttgta ttcatgatgg tcgccgtcgc
     8821 cggctgcgaa aaagcaccga ccgagggcgg agaagccaaa gacatcaaga ttcatgtagg
     8881 tgttgtagaa cccatctccg gcgcgcaggc agcgttgggc caggccgaat atgacggcat
     8941 tcagatggcc attgaaatga tcaatgaaaa aggcggcgtc atgggcaaat accccattac
     9001 ctttgacttt gccgattccc agagcgatcc cgctaccggt gcatccgaag ccgaaagatt
     9061 gatcaccagt aaaggcgtac ctgtcatcat gggttcctat tcatctgcca ttgctgcagc
     9121 catcagtgaa gtagctgaaa gaaacaaagt cgttctgtgg gaaatgtccg gctctgccga
     9181 taacctgctg caaaaaggct atcagtggac tttcaggaat gaagccaatg cttcggcctg
     9241 gggggcagaa tccgttgagt tcatcatggg caacgatgca cagatccagg agaaacttgg
     9301 caaacccgcc aaagaactgg tcgtagccat tgttcatgaa gacggcccct acggtaccgc
     9361 cgtagccgaa ggcaaccaag ccaccgctga aaagtatggc ctgaaagtcg ccatgaatga
     9421 agcatactca gccaaagccg ttgacctgtc cagcatcatc atgaaaatta aatcatccaa
     9481 tcctgatgta ttgctgttaa ccagctatgt caatgatggc attatgttta accgccaggc
     9541 caaagaactt ggcttgaaag tgccgatctt catcacccat agcggcggcc acagcgtaca
     9601 ggctttcgtt gacggggtag gcgcagatgc caactccatg ctgacggttg acccggttcc
     9661 ctgtaatccc aataccgctg gattctctgc ggaatatgca gctatctttg atgatttcgt
     9721 aaaacgttgg actgataaat ttggcgttcc tccttatcat catgtagaat atcgccagtt
     9781 tgcccagacc atgctgttct tcaacgaaat cgttccggca gccattgaaa aatccggtga
     9841 gttcagtgcc caggcagtag ctgatgccat cagagctgct gatgtacccg ccgaaaacag
     9901 cctgtgcgga ttcggcgcta aattctccac tccggagagt ccctggaccg acccctggtt
     9961 aggcaacaaa cacaccggcc agaactatga agcccgtgca tttatcaacc agtatttcga
    10021 cggcacattg gtaactgtat ggcccgaaaa cctggcaaaa caaaaagccg aactgttcct
    10081 gccggccagt cacccgttat ctgccaataa ataagctgac tttcgaagat aaggctgtcc
    10141 tcgagttcag ctgacagaaa agctcccccc acccccgggg ggagcttttt cacgccctcg
    10201 cgcaaggcaa gaaccgtccc cgacttgcgc aagtcgggga cggttcttgc cttgctttct
    10261 gagtgtgggt tggtctgcgg atgatgcagt agaccgcagc taaattcaga aaattccata
    10321 agttaatacg cctgacgtat taacttatgg aattttctga aagtttattg ggctggcgct
    10381 gatgattcct gaggttcgaa ggtggacagg aaagggccgt ccggtctggt gcggcggcgg
    10441 taggcgagat aaaaagcaat ggcgcgcagc agccagtcgg agatgaagac cagccaggcg
    10501 atttgaatgg gctgcgccag tacgataatg accagataca gcatgggaat gcggtacgcc
    10561 caggtcgcca ggacggtgat caccatgggg gtacgggtgt ccccggagcc tttcaggatg
    10621 ccgcccatta ccatgctgag cgcaatcgtc aattgttcca gtgcggcgat gcgaatgaga
    10681 agtccggcta tggcgatgat ggcagggtca tgcgtgaaaa gccgggcgat ggggaagggg
    10741 aacagggcaa acagcaaggc aaaaaagccc ataaacagca ggccaaactc aatcgtgccg
    10801 cgcgtggcct gcagcaggcg ctccttgtcg cgcgctccca cgctctgccc caccagggtg
    10861 gtcgcggcaa tggcaatgcc aaagcccggc atataggaga tggattccac gatgaccccc
    10921 acctggtgag ccgcgtagga caccgtaccg gtgaaaacga tcaggtagac cgcaatcata
    10981 atgctcaggt tattaaaaaa ctgctctaca aaactaggca gacccagcga aaaaattcgc
    11041 ttcaatacct gtcgctgcag ccgtctgatt gcgtcccagc gcagcttgag cccacccccg
    11101 ccgcggatca ggatagtgaa aataatcaaa cagccgacta catgcgcaat cgccgtggcc
    11161 agcgctgaac ccgccacgcc cagagccgga aaaccaaact tgccgtagac cagcacatag
    11221 caacctatgc agttgacgat gttcagcacg atcgccacat acataggaat ctccgtgcgc
    11281 ccccagccgc gataaagcga gctcagcatg gtataaagca cccccagcgg ggcaatcgca
    11341 aacgtaatct gcacatagcg caccgccagt gcatgcaccg ccggttccac ccggaaaagt
    11401 ccaaccaaga aatcagcgta atagactccc aggaacccgc tcaggcaacc catcagcaat
    11461 cccagcagca gcgtctgacc ggcgatttgc tctgcttcgt ggacattgcc tgccccccgc
    11521 gcctgggctg ccaacaccgc cgcgccgata cccagcgccc ccaggaaaag aatcacttca
    11581 aaaaagattt ccgcgccaag gctgaccgcc gccagcggca ccgcgcccag ccgtcccacc
    11641 accgctacat ccacgatgcc gatcagcata tagagaatca tttccagcat cgccggcagt
    11701 gccgtccgct gaatcgtcgc ccgcagcggt ttgtttcgta aaagcaacag cattccttgc
    11761 atctccatcc aataacgtca gcgttagcct gccaaatatt cgaaatattc cataagttta
    11821 tacgtcaggc gtattaactt atggaatttt ctgaaaacga ataaggggtt gcgtttgccg
    11881 tcacggcaag aaccgtacct agtatagcaa aaggaaaaat atatgcaaac caagaagagg
    11941 aattagcgcc tttcatatgg aatgtacatc ataaaagacg catcattgac gcagacgccg
    12001 ctgtggggca gcgttgcgca acatcaacca atcgacaaga ggaggaatca acgtggaata
    12061 ccagcagata aagtatgaag tcgaagaccg catcctgacc atcaccctca atcgccccga
    12121 acagatgaac acctacaatt ataccctgca ttatgaattg atcgatgcct ttgaccgggc
    12181 tgacgaagat gacaacatcc gcgccatcat catcaccggt gccggcaaag ccttctgcgc
    12241 cgggatggat ctgagcgaaa aaggttccag cttttcccct ggttccatgc ccatcgaaga
    12301 gtttcgcgac gggggcggga tgatggccct gcgcatgttc caatgcaaaa agccgctcat
    12361 cggcgccatc aacggaccgg cggtaggatt tggttttacc ttcacgctgc ccatggatat
    12421 tcggattggc tccaccaaag ccaaaatggg gatggttttc gtacgccgcg gcgtattgat
    12481 cgacgcctgc agttcctggt tcctgtccaa agtgctgggc atcaaccgcg ccctggaatg
    12541 ggctatgacc ggcaaggttt acagcgccca ggagttatac gaaggcggcc tgtttaatta
    12601 tctggtcgaa ccggaagaac tgatccccaa agcccgcgaa attgccaaag aaatcgctga
    12661 aaacactgct ccagtatccg tggccattac ccgccaactg atttggacca tggccggcgc
    12721 ggagcatccc atgcaggccc atcgcatcga atccaaaggg ttccactggg ccggccagca
    12781 gcccgacgtg gttgaaggca tcatggcctt cctggaaaag agaaaaccca attactccat
    12841 gagtgtcaaa gacggcatgc ccccctttta tccctggatg cccgagccgc cttttaaaga
    12901 agaaaaataa tatcgcttac aaaaacatat catcacgcgt gataacgcca aaaagacttc
    12961 aggcgcgggt gttggaccgc ctattataca actagagcag cactgaagta ttgccccaga
    13021 gagccggtca gtatttataa cggctggaca atcatacaac ctgaccagca cattatcatc
    13081 tcgcctgtgt aagcagggaa gacattcgtt atttgaaacc gggaatatat tcccggtttt
    13141 ttaaaaaaat agtgacaaga aatgcccgat cccacagtca actttatcga aaggatgggg
    13201 aaaatgaagc caactgtttt gttgtttgct tttcaagatg cagaaaaaag ggagaaaatg
    13261 gcccgcgcgt tgctgccact caagctgcgc atcaaaaaga ttaacaaaag cgattatcat
    13321 cagccgttgg gggcgctgct gggcatcgcg gcggaggagc ctgccatcgc cgcctaccag
    13381 ggacccgatt tgccccatga aatgatgctg atcgccggct gcagcgatcc acaaatccac
    13441 gccttgttgg acgccctaag gaacgccggt attccccgga ttcatcacaa agccgtcctg
    13501 accgagaaca accagtactg gaacgcgttg gagctgtatg aggaactgaa tcaggaatat
    13561 gaatacttcc gccagcagga aggccaataa tcaacgccag tagggatcgt cgctatgatt
    13621 tctttgttcc atcagccctg tcaattagga acatagtaag aatgaagtca atcccatagc
    13681 ccgctgagat ttccggggtt gatttttcaa gaaactgagc ttgctctaaa aatgacagct
    13741 tcaaagcgta ataccgacgt tctagtggtg tgatatcggt ataatacaaa caaaggaggg
    13801 atatctggtg ttattcagaa aaatactgcg ggtaatcttt cgcctgttta ttgccaggat
    13861 tctggttgga ggaaaaagcg aacaaaagga cggataagcg caagcaaaaa ctgtccccaa
    13921 ttttcgcgaa ggcgggaaga aactatccgg gcaagaacat tcccgggcga aaattttcaa
    13981 cccgaggaga acagccgatg aaagaaaaaa tgagatcaac ctttgacgca ttcgccgacc
    14041 tgctcgacga tgccgtagac cgcatcccgg atcggttttt gcgtgatctc accggcgggt
    14101 ttaacctgcg ccgcgaggcc aaacgcgaag acgattattt catcctgggc gagtatgtag
    14161 aggacggctt gggcggatgc ttcatcgtct tttattacgg ttcgtttgca gtcctgttgg
    14221 ccgatgagcc cgtcaccgtt tgggaagcgg aaatcatcga caccgtgctg cacgaaatgc
    14281 agcatcatct ggaggccatg gccggccgcg atgatctcgc ccaacaggaa atggaggaac
    14341 tggaacgcgc tctgcaaaaa ggagagtagc cgcgcaaggc aagaaccgtc cccaacttgc
    14401 gcaagttggg gacggttctt gccttgcgcg ggttcgcctg gcgcagacag aaggcccttc
    14461 ccctcttata tcttgccaga tgattataat tgatatagca atacttgttc taaaaacgat
    14521 acgggtacaa aagtgcgatc attcataata atcggaacag cttctaaaat gtagggtatc
    14581 ttatggttgg cgatatatat attatcatcg attttcagct tggcggtggc ctggcccttg
    14641 gttagcataa cactgcgttg cgcagcattc caatctaccg tatagcctaa atgctcacct
    14701 acttgacgca aagggatcat caccgtttgg gtttcagcgt ttaggaccgt cttaaatacc
    14761 aagcccgcaa taacgatttc gccatcttgc gcggcaacac ttttcttcag atcagcccgg
    14821 ttaacagcaa attcaggaat acccattgct ccgggggcta tttcatattt gggaaaaaca
    14881 atgacgatat cattgtcctt taaataaaaa ccctgggtat cgctgattcc cttaaaaccc
    14941 atgtctcctt cgaaaaacat tacattatca tcctgagagc gcaattcaat ctgcttggcg
    15001 atttcacgat tcatcacagc tttataatct gctccgggcg ggaacaaagc tgccagttga
    15061 aggggcttgt ttgccttttt gtcaatatta aagcaattta caactgtcat gccattcgct
    15121 ccgccggtaa aagtgtagta agtcatcgta aaagaaagaa atgcttcatc attcgattta
    15181 agatcataat caatagaaag cacgtacggc ctcatttccc aaccattctt tttcgcttcc
    15241 tggacaaatt catcggcttg catttcaata agatccttgc tatagataac ctgcttttta
    15301 atgaaatagt ttaattgttc ctggaaatca gcatcggtta atcctgagat gaccggatat
    15361 gtaatatcaa tttctactac atcatgcttc tccttgatga cctcaaaggt aatttggctg
    15421 tcctgtgtaa tggctgttac tgcctcggca gggagcatcg ttgcagggga ctcggccgag
    15481 gccggagagg caataaccgt cccgataaaa aacagactgc ctatcactcc caatacaatc
    15541 gtttgtctga ttttcatata gatacctcct ctatttgtat tgttaaagat gattacgaag
    15601 atttgtaaaa taagttacaa tggtaatctt tttaaacggg ggtatttatt tctggccgtt
    15661 cgcctccgga agccttttca gcgatgacgt cggaaacagt caatcactca aagacagccc
    15721 cagctttggc gcgtggtcgc gtgactcggc agcctattgc ataaagcaga agttgatggt
    15781 gggcaaccag ggaatggccg agccggaccg caatataacc cgagccgagg ccgtggtgat
    15841 ggttatgcgg atgttacaaa cggcagcatt gatctgacgc aagtcacgca agtcggggac
    15901 ggttcttgcc ttgctttctg ggggcagaaa gcaaggcaag aaccgtcccc
//
LOCUS       DAOTHZ010000051        15798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4477_length_15798_cov_6.757797, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000051 DAOTHZ010000000
VERSION     DAOTHZ010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_15798_cov_6.757797"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="P5549_07065"
     CDS             complement(<1..1065)
                     /locus_tag="P5549_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12868.1"
                     /translation="MKRIIIKLCLLVGLLLGLALLWLSAPAAAASAEYVTVRIVGTDS
                     LLLDNFVVDRASLTSYLQSHDLTPDDADPAPNALDAVLYASLHNGYDEDSYEIAYLFD
                     YGAYFIRRIAGVKAEGSDYWGSLAVNSEGGLDGGSLCAHAVQAGDTYIVYYDQYTGTG
                     ANYGNQAYACFRQEWLAGETGATITVDLMTAGYDEGWNTVAVPLAGATIYASGADLTD
                     RTAVAVTDSAGQACLSFAAAGTYLLYLSGDYTYTQCTVTIDGSNVTFHDVEIRVTDGS
                     NLLPEGALLLTDDDGKARSPWTIASGAYTYRLPGGTYHYAASASGCRPASGAIEVSGD
                     SSQDLSLTPFRGHAVTITLGA"
     gene            complement(1062..1865)
                     /locus_tag="P5549_07070"
     CDS             complement(1062..1865)
                     /locus_tag="P5549_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRW12869.1"
                     /translation="MQRQFEKPECWPGDTADPLQRIKRHPPLPVRTLLAAGIILLVVP
                     LLIALGMAVLDDRKYYFISLLIIIFAMIPFVMIFESRRPQAREIVIIAMLAALAIAGR
                     SLAFMLPGFKPVIALVIITGVCLGGEAGFLVGVITGFVSNFFFGQGPWTPWQMFCFGI
                     IGFLAGILFRQGRLSSRRMSLCWFGGLSALLLYGGIMDTANVLIYQGHLTRGLLLASY
                     ASGFGFNVVHALSTVFFLWLLAEPMIEKLERVKTKYGLLEDNKGKGDES"
     gene            complement(1799..3523)
                     /locus_tag="P5549_07075"
     CDS             complement(1799..3523)
                     /locus_tag="P5549_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW12870.1"
                     /translation="METYQINGLTFRYPLQTEPALKAIDICVQPGELILLCGRSGCGK
                     TTLLRHLKTVLTPHGNRSGRILFAGTALEQVDQRRQAQDIGFVMQSPDNQIVTDKVWH
                     ELAFGLESLGCNPRDIRLRVAEMASYFGIQDWFYQNVSELSGGQKQLLNLAAIMVMQP
                     AVLILDEPTSQLDPIAAADFLATLKKINRDLGTTIIMTEQRLEEALPLSDRVLVMDQG
                     RIVSDAAPRETGERLRTWQHEMFQAMPAPLQIHAGVKSASPAAPVTVGEGRQWLDTLA
                     LAPEPPGRPDPAPEAALQPAVIPALEVKNIWFKYEKNHPDVLKGLSLQVHQGQIQAVA
                     GGNGTGKTTLLHLLAGIVKPYHGKVLVFGQVRGLRGRQRNEARIAVLPQNPQALLVKK
                     TVALDLLEMLEENELSKEQRQQKMKAMAERVGLTELLARHPYDLSCGEQQRAALAKVL
                     LLEPQILLLDEPTKGLDSHFKAALGDILKSLAAEGVAILMVSHDIEFCARHADRCALL
                     FDGTVVTENTARAFFAGNSFYTTAANRMARHLYPDVVTSEDVIAKCSANLKNQNAGPA
                     ILLTRSKG"
     gene            complement(3496..4386)
                     /locus_tag="P5549_07080"
     CDS             complement(3496..4386)
                     /locus_tag="P5549_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRW12871.1"
                     /translation="MSDAFSRRHPLINFIYFTLIIGFSMFLMHPLCLAISLLGAVSYA
                     LCLKGRKALRFLVCAMLPLLLVTAWLNPLFNHAGVTLLFYLPGGNPVTLESMVYGLAA
                     ATMFVTVVVWFSCFNAVMTSDKFVYLFGEIIPALSLIFSMVLRLVPRLKAQSRVIANA
                     RKGVGRDPGSGPLLQRARSVVQIISMMITWALESAIDTADSMKSRGYGLAGRTSFSLY
                     RFELYDKWLLAAMLLLAGFILAGAGSGLIRADYFPALQIEMPTLLSSMVYMAYAGLGL
                     LPVLVDGGEDMRWKRIKSTA"
     gene            complement(4383..5102)
                     /locus_tag="P5549_07085"
     CDS             complement(4383..5102)
                     /locus_tag="P5549_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HRW12872.1"
                     /translation="MRTNRQKWILVIAVLLGLLSLAWGWGGGLPEKDQGQQAPVPTIQ
                     RASDDQQAAAPGTKAGAANHHEEENLPVVETGQKALPVNEPTSGLGAAAETKADRTPA
                     TCTLTVQCTTIFDHLDRFDDRKRQVLPPDGIIYPVTGIVFTPGESVLDVLQREMKKAG
                     IHLETTAVPIYNASYIEGIGNIYELDCGELSGWVYKVNGLVPNYGCSRYLLQDGDRVE
                     VVYTCDLGRDVGGSNFGEERP"
     gene            complement(5118..6755)
                     /locus_tag="P5549_07090"
     CDS             complement(5118..6755)
                     /locus_tag="P5549_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW12873.1"
                     /translation="MKNKQKKSRIMATGLIVIMMFVWPAAAWGDESSDQLQTALPATG
                     TWLVRTVNQPTCSTVGGEWAVLGLSRSGYAVPPGWYDIYYQNVLAALQAADGVLSTRK
                     YTEYSRVILALTAIGKDPADVGGYNLLTWLGDYDKVVAQGINGPVFALLALDSGAYAM
                     PVCVPAATQASREMYIDYILARQLENGGWSLTGHAAEADVTAMVLQALAKYQADLRVK
                     TALERGVLALSGLQDETGGFASGGTANVESTVQAITALGELKIDPCSSLFTKNGHTLL
                     DSLLSYAVPGGGFKHIAAQTEADIMGSEQGFYALVAYQRFLHGQRSLYDLSEEIKATV
                     EKLETGTGLAGKHADVKAVGVSRPGRTFKDIQGRDGQAAIEALSAREIVNGVGNDAYE
                     PQRSLTRAEFAAIVVRALGLTPAVDTIFADVPADSWYAAYVGAAYRYGIVSGTSAATF
                     TPTATITRQEAAVMTARAARLCGLANDMNEPAIEDTLDRFSDAASCADWAKASLAFCH
                     RQGILPAEQKKLEPGTAVTRAEMADMLYRMLVQAELI"
     regulatory      complement(6885..7076)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7376..8824
                     /locus_tag="P5549_07095"
     CDS             7376..8824
                     /locus_tag="P5549_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRW12874.1"
                     /translation="MFFFSRFLAFIKTHPRIKTTGVFCTGLLAALLAVWLLGYSTVKI
                     EGVTMKAALAPSASGITELNFPPFGVITAPTHQGPVKLSLTLEQIDSRLLNGQLNHAA
                     DSKALLLRLQKGIKASVYDFALRQLLIALVVAWVMILLVWRTNWKTALLHAVLGTALL
                     AMPVVYAISTYDTNAFREPEYKGVLSMAPSAIEFVSNSLSDLHIMKENTDKIVTNFRK
                     LFLHADALMVMADAEEQQKMVKVLLVSDLHSNPVGVEFIKSTAHRFQVDFIINAGDLT
                     DLGTATEFEAIRELQSIPVPQLLAPGNHDSPEIIDIIAQLDNVTVLDGQMLTVAGIKV
                     IGFADPLADDAAVEYENPEKTRQILAEEAVRIQAGLEDQERPDILVVHHPSLGEKLMS
                     LAEITVSGHNHQLQIKQEEGSTFINPGTAGAAGLRGLYSEEGRAYSAVIAYLIPGSGL
                     MAMDLINYEPASKQFSLHRKVIQPADTLEATP"
     gene            complement(8930..11050)
                     /locus_tag="P5549_07100"
     CDS             complement(8930..11050)
                     /locus_tag="P5549_07100"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRW12875.1"
                     /translation="MDISRIKKRDGRIVPFETDKIAQAIFKAAKAVGGKDFSIAQELA
                     DKVIEIAQYVDDDEIATVEGIQDLVEKVLVKNGHYKTAKAYILYRKQHDDIRKAGQLF
                     LNNQIIANYLEKNDWRVKENSNMTYSLQGMNFHISSLIVSQYWQDQIYTPQMKEMQSS
                     GDFHIHDLGILGVYCVGWDIHDLLLEGFKGVRGKVASGPAKHFRSILGQLVNFFFTLQ
                     GEAAGAQAFSNFDTLLAPFIYYDKLDYKQVKQGLQEFVFNMNVPTRVGFQTPFTNITM
                     DLKVPEFMKNEPVIIGGKYMDKTYGEFQAEMDMLNQAFAEVMMEGDCEERVFTFPIPT
                     YNITKDFDWSNPHYDKIWEMTAKYGIPNFSNFVNSDMNPEDARSMCCRLRLDNRELRK
                     RGGGLFGANPLTGSIGVVTINMSRLGYLSKDKEEYFSRLADMMDIARDSLEIKRKVLE
                     NLTESGLYPYSQFYLRNVKAATGQYWKNHFSTIGLVGMNESCLNFLGQDIASPEGLAF
                     SKEVMDFMRDRLVAYQEESGNIYNLEATPAEGVSYGIARKDKEAYPKIIVANEAEYKK
                     GAEPYYTNSTQLPVKYTDDLFEALQIQDELQTRYTGGTTFHVFLGESISSVESVKKLV
                     GKVCHQFRLPYFTITPTFSICPSHGYLSGEKYSCPICEAEGKQTACEVYSRVVGYIRP
                     IDQWNAGKQEEFKERRLFDKALSH"
     gene            complement(11338..12546)
                     /locus_tag="P5549_07105"
     CDS             complement(11338..12546)
                     /locus_tag="P5549_07105"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRW12876.1"
                     /translation="MDKVVLAYSGGLDTSIIIPWLKENYGYEVIAFCADVGQGDELDP
                     VREKALRSGASKVYIEDLTEEFVTDYVFPVLKAGAIYEKKYLLGTSFARPLMAKKMVE
                     IAEQEGAIAVCHGATGKGNDQVRFELGVKAFNPSLKIIAPWREWSIRSREDAIDYAHA
                     HGIEVPVSKEKIYSRDRNIWHISHEGGDLEDPANEPKNEMYMLTVPPEDAPDVPAYVE
                     ITFEQGIPVAIDGQAMGAVALLSELNRLGGANGVGIVSLVENRLVGMKSRGVYETPGG
                     TILYTAHEELERLTLDRRTLQFKEQMAVKYAELVYDGNWFTKLKAAMDAFVDETQKNV
                     TGKVKVKLYKGSCTAVASVSPYSLYLEDLATFSADDIYSHKDAEGFINLFGLPLKVQA
                     LLNQKLTGGK"
     gene            complement(12692..13642)
                     /gene="argF"
                     /locus_tag="P5549_07110"
     CDS             complement(12692..13642)
                     /gene="argF"
                     /locus_tag="P5549_07110"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRW12877.1"
                     /translation="MEVMEVSQALKGRDFISLHDFSAEEITLILETGLQLKKEQKAGT
                     PHPILAGKTLGMIFQKSSTRTRVAFEVGMYQLGGHALFLSPRDIQLGRGETIKDTALV
                     LERMLDGIMIRTFSHDEVLELAHWASVPIINGLTDLLHPTQVIGDMMTIIENKGSLQG
                     IKLAFIGDGNNVAHSLLYGGAKTGMDVVVACPQGFEPDAGVLRQAQADAQATGAKLWV
                     VEDPVEAVSGADVVYTDVWASMGQEAEAAEKEQKFLRYQVNRELMSHAHRDAIVLHCL
                     PAKRGKEITDEVMDGPQSVVFPEAENRLHAHKAIMALVMQ"
     gene            complement(13676..14866)
                     /locus_tag="P5549_07115"
     CDS             complement(13676..14866)
                     /locus_tag="P5549_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRW12878.1"
                     /translation="MTNKDIAERGQKVVMNTYGRFPIAPVKGKGSYVWDADGKQYLDF
                     IGGIAVCALGHCHDELQQVIRQQAASLWHVSNLYWIEPQVRLAEKLVSISGLDKAFFC
                     NSGAEANEAAIKLARKYFYRQQESGRTQIIVFNESFHGRTLATLTATGQKKYQEGFAP
                     LPSGFVYADFNNLASVEKLISDDTAAIMIEPIQGEGGVHPADTVFLNSLRRICDREGI
                     LLIFDEVQCGVGRSGNFMAYQNYQVKPDIVTMAKGLGGGFPIGAMLASEKAAGGFAPG
                     DHASTFGGNPLATAVADRVVELVSAPAFLDNVNKAGRSLKESLQSIQDERMVTVRGRG
                     LMLGAEFNTPIKDLVTICMHNGLLLLGAGANVMRFVPPLNINETEINQAVAIFKKSLR
                     EWKV"
     gene            complement(14942..>15798)
                     /gene="argB"
                     /locus_tag="P5549_07120"
     CDS             complement(14942..>15798)
                     /gene="argB"
                     /locus_tag="P5549_07120"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRW12879.1"
                     /translation="SAMEKAEVLVEALPYIKDFYGKRVVIKYGGAAMTECDLKMKVMQ
                     DIVLMKYVGMHPIVVHGGGPDITGMLGRLNMDTEFVNGLRVTDRETMEVVEMVLGGKV
                     NKEIVSGLNASGGKAVGISGKDGGLIKARKVDPSGKLGFVGEVDTVNPQIIETLIENG
                     YIPVIAPIGIDDEQQSYNINADLVAASIAVGMKADKLVLLTDVPGLLRDKNDSRSLIS
                     VLKVSQVPDYVEEGIIAGGMIPKVQCCVEAVTGGVARTHIVDGRVPHSILLEIFTNEG
                     IGTMVVNE"
BASE COUNT         3679 a         4415 c         3854 g         3850 t
ORIGIN      
        1 tgcgcccagg gtaatggtca cggcatgccc gcgaaacggg gtcagggaaa gatcctgcga
       61 gctatcccca ctgacctcaa tcgcaccgct agcaggccgg cagccggaag cgctggcagc
      121 ataatggtag gttccgcccg gcaaccgata ggtataggcc ccggaagcga tggtccaagg
      181 agaacgagcc ttgccgtcat catcggtcag taacaaagcc ccctcaggca aaagattgct
      241 cccgtctgtc actcgaatct ccacatcatg gaacgtcaca ttgctgccat cgatggtaac
      301 cgtgcattgg gtataggtat aatcaccgga caggtatagc aaataggtgc ccgccgccgc
      361 aaaactgagg caggcctggc ctgcgctgtc ggtcactgca accgcagtgc gatctgttag
      421 atcggctccg ctggcataaa tggttgctcc cgccaagggg actgccaccg tattccaccc
      481 ttcatcatag ccggcggtca tcaaatccac cgtgatggtt gctccagttt cccccgccag
      541 ccattcctgg cggaagcagg cataggcctg attgccgtaa ttggccccgg tcccggtgta
      601 ttggtcatag taaacaatgt aggtatcgcc ggcctgaacc gcatgcgcgc acagactgcc
      661 gccgtccaga ccaccttccg aatttaccgc cagcgaaccc caatagtcag agccttccgc
      721 ctttaccccg gcgatccggc gaataaaata agcaccgtaa tcaaacaaat aggcaatttc
      781 ataactgtct tcatcatagc cgttgtgcaa gctggcatag aggactgcat ccagagcgtt
      841 gggggccggg tccgcatcat ccggagtcag gtcatggctt tgcaaatagg atgtcaggga
      901 cgccctgtcc acgacaaaat tatccagcaa caggctgtcg gtgccgacga tccgcaccgt
      961 gacatattct gccgacgctg ccgctgccgg cgcgctcagc cacaacaggg ccaatccgag
     1021 cagcaatcca accagcaagc ataattttat gatgatcctc ttcacgattc gtctcctttt
     1081 cctttattgt cttcgagcaa gccgtatttg gtcttgaccc tctccaattt ctcaatcatc
     1141 ggctcagcca gcaaccacag aaagaatacg gtggacaagg catggacaac attaaaccca
     1201 aagccggagg catatgaggc cagcagcagg ccccgggtga gatgcccctg ataaatcagt
     1261 acattggccg tatccataat cccgccatag agcaacaggg cagacagtcc gccaaaccag
     1321 cataaagaca tacgcctgct gctaagccgt ccctgtctga acaatatccc ggccagaaac
     1381 ccaataatcc cgaaacaaaa catctgccat ggcgtccatg gcccctggcc gaaaaaaaag
     1441 ttggaaacaa agcctgttat gacgccgacc aggaatcccg cttccccgcc caggcacacc
     1501 ccggtaataa tgaccagggc aataaccggt ttaaagccgg gcagcataaa agccagggag
     1561 cgaccggcaa tggccagggc ggccagcatg gcaataatga caatttctct cgcctggggg
     1621 cgacgggatt caaaaatcat cacaaagggg atcatggcga agatgatgat cagcagactg
     1681 atgaaatagt attttcggtc atccagaacc gccatcccca gggcaatgag cagcggcacc
     1741 accagcaata tgatccctgc cgcaagcaag gtgcgaaccg ggaggggcgg gtgccttttt
     1801 atcctttgga gcgggtcagc agtatcgccg ggccagcatt ctggtttttc aaattggcgc
     1861 tgcattttgc aatcacatcc tcacttgtga caacatcggg gtaaaggtgt cgggccatgc
     1921 ggttggcggc ggtggtataa aaactgttgc cggcaaagaa cgcccgcgcc gtattctcag
     1981 tcaccaccgt gccgtcaaaa agcaaggcac agcgatctgc atgacgggca caaaactcaa
     2041 tatcgtggga taccatcagg atagccaccc cctcagccgc caatgatttc aggatatctc
     2101 ccagggctgc cttgaaatgg ctgtccagtc ctttggtggg ctcatccagc agcaaaatct
     2161 gcggttccag caacaacacc ttggccaagg ctgcccgctg ttgctctcca caggataggt
     2221 catagggatg gcgggcgagc agttccgtca ggccgacccg ttctgccatc gccttcatct
     2281 tttgctgccg ctgttctttg gagagctcat tttcttccag catttccagc agatcaaggg
     2341 ccaccgtttt tttcaccaaa agcgcctgcg gattttgcgg caagacggct atcctggctt
     2401 cattccgctg ccggccccgc aagccccgga cctgaccaaa caccagcacc ttgccatgat
     2461 aaggttttac gatgccggcc agcagatgca gcaacgtcgt tttgccagtg ccgttaccgc
     2521 cggcgacggc ctggatttgg ccctgatgaa cctgcaggga caaccctttt agaacatcgg
     2581 gatggttttt ttcatatttg aaccatatgt tttttacttc gagggcggga ataaccgccg
     2641 gttgcagcgc tgcttccgga gctggatcag gcctgccggg cggctccggg gcaagcgcca
     2701 gcgtatccag ccattggcgc ccttcgccta ccgtaacggg agcggcaggt gaagcactct
     2761 taaccccggc atgaatctgc agaggagcag gcatggcctg gaacatttca tgctgccacg
     2821 tccgcagtcg ctcaccggtc tcccggggag cggcgtcact gacaatccgg ccctggtcca
     2881 tcaccagcac ccggtcggac aggggcaggg cttcttccag tcgttgctcg gtcatgatga
     2941 tggtcgtgcc caggtcccgg ttgatttttt tgagggtggc cagaaaatcg gccgcagcga
     3001 tggggtccag ctgactggtc ggttcgtcca aaatcagcac tgccggctgc atgaccatga
     3061 tggcggccag attgagcaac tgcttctgtc ccccggagag ttcgctgaca ttctggtaga
     3121 accaatcctg gatgccgaaa taactggcca tttccgccac ccgcagacgg atgtcccggg
     3181 ggttgcagcc caggctttcc agcccaaagg ccagttcatg ccataccttg tcggtgacga
     3241 tctgattgtc gggactttgc atgacaaagc caatgtcctg cgcctgcctg cgttgatcga
     3301 cttgctccag cgccgtgccg gcaaaaagaa tccggccgct tcggttgcca tggggggtca
     3361 aaaccgtttt taaatggcgc agtaaagtgg ttttgccaca gcctgagcgg ccgcagagca
     3421 gaatcaactc gcccggctgg acgcatatat caatcgcttt aagggccggt tcggtttgta
     3481 agggatagcg gaacgtcaag ccgttgattt gatacgtttc catcgcatat cctcccctcc
     3541 gtcaacaaga accggcagca accccagtcc ggcataagcc atatatacca tgctgctcag
     3601 cagcgtaggc atctcgatct gcagggcagg aaaataatct gctctgatca gcccgctccc
     3661 tgctccggcc aggatgaaac ctgccaataa aagcatggcc gcgagaagcc acttgtcata
     3721 cagttcgaaa cgatacagag aaaagctggt gcgtccggcc aaaccgtagc cccgtgactt
     3781 catactgtca gcggtatcaa tcgctgactc caacgcccag gtgatcatca tcgaaatgat
     3841 ctgcaccacg ctgcgcgccc gctgcaggag agggccgctg cctgggtcgc ggccgacacc
     3901 cttccgggca ttggcaatca cccggctttg tgcctttaaa cgtggaacga ggcgcaatac
     3961 catggaaaag atcaaagaca gcgcggggat gatttcgcca aacaaataaa caaatttatc
     4021 cgaggtcatg actgcgttga aacagctaaa ccagacaacg actgttacaa acatggtggc
     4081 ggcggccaac ccgtaaacca ttgattccag cgtgaccgga ttgccgccgg gcaaataaaa
     4141 caagagcgtc acaccggcat ggttaaaaag cggattcagc caggctgtca ccagcaacag
     4201 agggagcatg gcgcaaacaa gaaagcgcag cgcttttctt cccttcaggc agagcgcata
     4261 actgacggca cccagcagcg aaatcgccag acagagcgga tgcataagaa acatggaaaa
     4321 gccaataatc agcgtgaaat agataaaatt gatcagaggg tggcgccggg aaaaagcatc
     4381 actcatggcc gttcctcccc aaaattggag ccgcccacgt cccggcccag atcacaggta
     4441 tagactactt caacacggtc accgtcctgg agcagatagc ggctgcagcc atagttgggg
     4501 accagaccgt tgactttgta cacccacccg gagagttcac cgcaatccaa ttcataaata
     4561 ttgccgatcc cttcgatata gctggcgtta tatatgggca cagccgtcgt ttccaaatgg
     4621 atgccggcct ttttcatttc ccgctgtaat acatccagga ccgattctcc cggtgtaaaa
     4681 acgatgccgg tgaccggata aatgatgccg tccggcggca gcacctgccg cttgcgatcg
     4741 tcaaaccggt ccaaatgatc gaaaatagtg gtgcattgta cggttaacgt gcaggtcgcc
     4801 ggggtcctgt cagccttcgt ctctgctgct gcccccagcc cggatgttgg ctcgtttacc
     4861 ggtaacgctt tctgccctgt ctcaacaacc ggcaggtttt cttcctcatg gtgattggct
     4921 gctcctgcct ttgttccggg agccgctgcc tgctgatcat cgcttgccct ctgtatcgtt
     4981 ggcaccggtg cctgttggcc ttgatccttc tccggcagac cgccccccca gccccaggcc
     5041 aggctcagca atcccagcag gacagcgatg accaggatcc atttctgtct gtttgttctc
     5101 atctttcctc cgcttgctta tatcagttcc gcctgtacca gcatccggta cagcatatct
     5161 gccatctccg cccgggtaac ggcagttccg ggctccaatt ttttctgctc tgccggcagt
     5221 atcccctggc gatgacagaa ggccagtgat gctttggccc agtcggcgca agaggctgca
     5281 tcgctgaacc ggtccagcgt atcttcaatg gccggttcgt tcatatcgtt tgccaaaccg
     5341 cacagccggg ctgcgcgggc ggtcatcacc gctgcttcct ggcgggtaat ggtcgctgtc
     5401 ggagtaaagg ttgcggcgga cgtgccggat acgattccat atcgataagc tgccccgaca
     5461 taggctgcgt accagctgtc tgccggtaca tcggcaaata ttgtatccac tgccggagtc
     5521 aagcccaggg ctcgcaccac gatggcagca aactcggctc tggtcagact gcgctgcggc
     5581 tcataagcat cattgccaac cccgttgaca atttcgcgtg ccgacaatgc ttcaatggcc
     5641 gcctgcccat cccgtccctg aatatcctta aaggttcgcc cgggccggct caccccgacc
     5701 gctttgacat ctgcatgttt gcccgccaag ccggtgccgg tctccagctt ctccacagtc
     5761 gcttttattt cttcagataa atcatacaaa ctcctttgcc cgtggagaaa ccgctgataa
     5821 gcgaccaggg cataaaaccc ctgttccgac cccatgatgt cagcctctgt ttgcgcggcg
     5881 atatgtttga aaccaccgcc cggcacagca tagctcagca gactgtccag cagcgtatgg
     5941 ccgttttttg tgaacaaact gctgcaggga tcgattttta attcacccag cgccgtgatg
     6001 gcctgcaccg tactctctac attggccgtc cccccgctgg caaagccgcc cgtctcatcc
     6061 tgcagtccgg acagggccag cacgccccgt tcaagggccg tttttacccg gaggtcggcc
     6121 tgatatttgg ccagcgcctg taacaccatc gcggtcacat ctgcttcagc agcatggccg
     6181 gtcaggctcc atccgccatt ctcaagctgg cgggccaaaa tatagtcgat atacatttcc
     6241 cggctggcct gggtggcagc cgggacacag accggcatgg cataagcacc gctgtccagg
     6301 gccagcaggg caaataccgg gccattgatg ccctgggcta caactttgtc atagtccccc
     6361 agccaggtta acaggttgta tccgcccaca tctgccggat ccttgccgat ggcggtaagg
     6421 gctagaatta cccgtgaata ctcggtgtat ttcctggtac tgagcacccc gtctgccgcc
     6481 tgcagagctg caagcacatt ctgataatag atgtcatacc acccaggcgg aacagcgtat
     6541 cctgaacggg acagtccgag cacggcccac tctccgccta ccgtactgca ggttggctgg
     6601 tttaccgtgc gcaccagcca ggtacccgtt gctggcagcg ctgtctgcag ttggtcactg
     6661 ctctcatccc cccaggctgc agccggccag acaaacatca tgatcacaat caatccggtt
     6721 gccatgattc tgcttttctt ttgcttgttt ttcatatcat actccccatt tttaatacca
     6781 ggttattcca cccgcataaa aaaccggcac acagacaaaa actccttccc ataggaagga
     6841 gcagactgat atatccagct tcatcgtcct atccttcgta ggttaaatga tgaacgagag
     6901 caggcaggtc tcctgacttg ggatcatctg cgtcctcagg ccttcccggc agttgctgcc
     6961 agtggcatat tttctgaagc gcatcccctt tacagtgacg ggatcgtgcc ggattcacac
     7021 cggcttccct tttaagtctg ccccgcaggg caaacacctg tcccggaata ttcaattgat
     7081 cggccggttg gtctgcctcc ggacagttcg gccagacaaa gataaatgat tcctgtattg
     7141 acaggataat caatttgatc cttttggtca agttggcgca tcacttgctg ataaaaatac
     7201 tgctttctcg aaaaaaagcc atggcagcca gcacctctaa aatacctttt gccgatacaa
     7261 tcgttctttc cctcggcaac tggcaaaata attaatttcc ggcaaggaaa aacattgacg
     7321 catcgctctt cttggtgaaa tactaaaaat ggaacaggct gggggaggtt tcattttgtt
     7381 tttttttagt agatttcttg cttttatcaa aacccatcca cgtataaaaa ccaccggcgt
     7441 cttctgtacg ggtttgctgg ctgctttgct tgcggtgtgg ctgttggggt acagcaccgt
     7501 caagattgaa ggggttacta tgaaagcggc attggcacct tctgccagcg gtattaccga
     7561 gttgaatttt ccgccttttg gggtgattac cgccccaacc catcagggac cggtcaaact
     7621 gagcctgacg ttggagcaga ttgatagccg ccttttaaat ggccagctga atcatgcagc
     7681 cgacagcaaa gcgttgttgc tgcgcctgca aaaaggaatc aaagcctcgg tttatgactt
     7741 tgctttgcgc cagttgctga tcgccctggt cgttgcctgg gtcatgattc tgctggtatg
     7801 gcgcacaaac tggaaaacgg ctttgctgca cgctgtgctg ggtacggcct tgctggctat
     7861 gccagtggtt tacgccatca gtacgtatga caccaatgct ttccgggaac ccgaatacaa
     7921 aggggttctc agtatggcac cctctgctat tgaatttgtc agcaactccc tgtccgacct
     7981 gcatattatg aaagaaaata cagataaaat cgtcaccaat ttccgcaagc tgtttctcca
     8041 tgcagatgcc ctgatggtca tggccgatgc ggaagagcag caaaaaatgg tcaaggtttt
     8101 gctggtcagt gatctgcact ccaacccggt aggggttgaa ttcatcaaaa gtacggccca
     8161 tcgtttccaa gtggatttta tcatcaatgc cggtgatttg acggatttag gcacagccac
     8221 tgagtttgaa gccattcggg aactgcagag tattcccgta ccccaactct tggcacccgg
     8281 caatcatgac agtccggaaa tcattgacat cattgctcag ctcgacaacg taaccgtact
     8341 ggacggacaa atgctgacag tcgcgggcat caaagtaatc ggctttgccg acccgttggc
     8401 agacgatgca gcagtggaat acgaaaatcc tgagaaaacc cggcaaatcc tcgccgagga
     8461 agcggttcgc attcaagccg ggcttgaaga tcaggaacgt cctgacattc tggttgtgca
     8521 tcatcccagt ctgggagaaa aattgatgtc gttggcggag ataaccgtct ccgggcataa
     8581 tcatcagttg cagattaaac aggaggaggg ttcgactttc attaatcccg gaacggcagg
     8641 tgccgccgga ttgcgaggct tgtattcaga agaaggccgt gcctattctg ctgtgatcgc
     8701 ctatctcatc cccggatcag ggttgatggc tatggatctg atcaattatg agccggcctc
     8761 aaagcaattt tcgctgcatc gcaaagtgat tcagcctgct gacaccttgg aagcaactcc
     8821 gtaatcgttt tcaataacta catttagact ccacaggcaa tccctaaaaa agccctcgga
     8881 acctgatcaa ggtgtgccga gggctttgtt ggttttatta tgattcagat taatgggaca
     8941 gcgccttgtc aaacaatctt ctttccttga actcttcctg tttgccggca ttccattggt
     9001 cgatgggacg gatataacct accacccggg aataaacttc acaagctgtc tgtttgcctt
     9061 cggcttcaca gatgggacag ctgtatttct ccccgctcag gtatccgtga ctgggacaga
     9121 tactgaacgt cggggtaatc gtgaaatacg gcagtctgaa ttgatggcag accttgccga
     9181 ccagtttctt gaccgattcc accgaagaga tggattctcc caggaaaaca tggaaggtgg
     9241 tgcctcccgt gtagcgggtc tgcagttcat cctgaatctg caatgcttca aacaaatcgt
     9301 cggtgtattt gaccggcaat tgggtggagt tggtgtaata aggctccgca ccctttttgt
     9361 attctgcttc attggccacg atgattttgg gataggcttc cttgtccttg cgggcaatcc
     9421 cgtaagacac accttcggcc ggagttgctt ccagattgta aatgttgccg ctctcttcct
     9481 gataggcgac cagccgatcg cgcatgaagt ccataacttc cttggaaaac gccaggcctt
     9541 ccggactggc gatgtcctgg cccagaaagt tgaggcagga ttcgttcatg cccaccagac
     9601 caatggtgga aaaatggttt ttccagtact gaccggtggc ggccttgacg ttgcgcagat
     9661 agaactggga atagggataa agaccggatt cagtcagatt ctccagcact ttgcgtttga
     9721 tctccaggct gtcacgggca atatccatca tgtccgccag acgactgaag tattcttcct
     9781 tgtccttgga cagatagccc agtctgctca tattaatggt aacgactccg atcgaccccg
     9841 tcagcgggtt ggcgccgaac agtccgccgc ctcttttccg cagttcccgg ttgtcaagcc
     9901 gtaaacggca gcacatgctg cgggcatctt ccggattcat gtccgaattg acaaaattgg
     9961 agaagttggg aatcccgtat ttggcggtca tctcccagat tttatcataa tggggattgc
    10021 tccagtcaaa atccttggta atgttatacg tcggaatagg aaaggtgaat accctttctt
    10081 cgcagtcgcc ttccatcata acttcggcaa aggcttggtt gagcatatcc atttccgcct
    10141 ggaactcgcc gtaggttttg tccatgtatt taccgccgat gatgaccggc tcattcttca
    10201 taaattccgg cactttaaga tccatggtga tattggtaaa cggtgtctgg aacccgaccc
    10261 gggtaggcac gttcatgtta aagacaaatt cctgcagccc ctgtttgacc tgtttgtaat
    10321 ccagtttgtc ataatagata aagggggcca aaagggtatc aaaattggaa aaggcctgcg
    10381 ccccagccgc ttctccctgc agggtaaaga agaaattaac cagttgcccc aggatggaac
    10441 ggaaatgctt ggccggaccg ctggcgacct tgccgcgtac gcccttgaag ccttccagca
    10501 gcaggtcatg gatatcccaa cccacacagt aaacgcccag gatgcccagg tcatggatgt
    10561 ggaaatcgcc gctgctctgc atttctttca tctgtggagt ataaatctgg tcctgccagt
    10621 actgggacac aatcaatgaa gaaatatgaa aattcatgcc ttgcaatgag taggtcatgt
    10681 tggagttttc cttgactctc cagtcatttt tctccagata attggcgatg atctgattgt
    10741 tgaggaataa ctggcctgct ttacggatat catcgtgctg cttacgatag agtatgtaag
    10801 ccttggcggt tttgtaatgt ccatttttaa ccagaacttt ctcgaccaga tcctggatac
    10861 cttccacggt agcgatttca tcatcgtcca catactgggc aatctctata actttatccg
    10921 ccaattcctg ggcaatacta aaatccttgc cccctaccgc cttggcggct ttaaaaatcg
    10981 cctgtgcgat cttgtcggtt tcaaatggta cgattcgtcc gtctcttttc ttgatcctgc
    11041 tgatatccat gcccatcccc cttactcttt ataactataa accacaacat atattgcttt
    11101 atattttacg aaccactaga tggagtatat ttattatgct acgcaagaga aaaaatagca
    11161 agtgcttatt tacccatttt agataaaatt ccgaaaaatt atcccagatt acatcttatt
    11221 ttttattaat tcgtaaatga tcttatcatt ccatgtatcc cggacagaat aaagcagagt
    11281 cggaaaaatt ccaactctgc ccggttgtac tttttatttt ttatgacttt ctgtcgacta
    11341 ttttcctccg gtcagctttt gattcagcaa agcctgcact ttcaggggca gtccgaacag
    11401 gttgataaac ccttccgcat ccttgtgact gtaaatgtca tccgcgctga aggtggccag
    11461 atcctccaaa tagagtgaat acggcgaaac ggaggccaca gccgtacaac tgcctttgta
    11521 taatttaact ttgaccttgc cggtgacgtt cttttgcgtc tcatccacga aagcatccat
    11581 ggcagccttg agtttggtaa accaattgcc gtcatacacc agttcggcat atttgaccgc
    11641 catttgctct ttgaattgta acgtgcggcg gtccagcgtc agccgttcca gttcttcatg
    11701 ggcagtatat aaaatggtgc cgcccggagt ctcataaaca ccgcgggatt tcattcccac
    11761 caggcggttt tccaccaagg aaacaattcc gactccattg gcaccaccca ggcggttgag
    11821 ctcgctcagt aaagcaaccg ctcccatggc ctgtccatcg atcgccaccg gaatgccctg
    11881 ttcaaaagta atttccacat aggcaggaac atccggagca tcctcggggg ggacggtgag
    11941 catatacatt tcatttttcg gttcattggc cgggtcttcg agatcgccgc cttcatggga
    12001 gatgtgccag atattacggt cacggctgta gatcttttcc ttgctgaccg gcacttctat
    12061 cccgtgggca tgggcataat cgatggcgtc ttccctggag cgaatgctcc attcccgcca
    12121 gggggcaatg attttcaggg agggattgaa ggcctttacc cccagttcaa agcggacctg
    12181 gtcgttgcct ttaccggtgg caccgtggca gacggcaatt gcgccttcct gctcggcgat
    12241 ttcaaccatt tttttagcca ttaacggccg ggcaaaggaa gtgcccagca ggtatttctt
    12301 ttcataaatc gcaccggctt ttaaaaccgg aaatacgtaa tcggttacaa attcttcggt
    12361 caagtcttca atatatactt tggaagcgcc cgagcgcaga gctttttctc tgaccggatc
    12421 cagttcgtct ccctgcccca catcggcaca aaaggcgatg acttcataac cgtagttttc
    12481 tttcagccag ggaatgataa tcgatgtatc cagacctccc gaatatgcca atactacttt
    12541 atccaacttg tttgcctccc ttgctatatt gctgtattgt aatgattttt catcagtaaa
    12601 taaccaacaa ttgtcattct tggcgattgc cagagatgga acaaaactcc atttacatgg
    12661 ttggttgtgc ccacggtcag gatgggttaa tttattgcat caccagtgcc atgatggctt
    12721 tatgggcgtg caggcggttt tctgcctccg gaaatacgac tgactgtggg ccgtccatga
    12781 cttcatcggt aatttccttg ccgcgcttgg caggcaggca gtgcagtaca atggcatccc
    12841 gatgggcatg actcataagc tcacggttga cctgataacg caggaatttt tgttcttttt
    12901 cggccgcttc cgcttcctgg cccatgctgg cccaaacatc cgtataaacc acatccgctc
    12961 cgctcacggc ttcgaccgga tcttccacta cccacaattt ggctccggta gcttgggcat
    13021 ctgcctgcgc ctgacgcagc accccggcat ccggttcaaa gccttgggga caggcaacca
    13081 ccacgtccat gccggtcttg gctccaccgt aaagcaggga atgagccaca ttgttgccgt
    13141 caccgataaa agccagttta atgccctgca gactgccttt gttttcgatg atggtcatca
    13201 tatcgccgat cacctgggtg ggatgaagca aatcggtcag cccgttgatg atcggaaccg
    13261 aggcccaatg tgccaattcc aggacttcat catgagaaaa ggtcctgatc atgatcccat
    13321 ccagcatccg ctccagcacc agcgccgtgt ctttgatggt ttccccgcgt cccaattgga
    13381 tatcgcgcgg gctgaggaaa agggcatgcc cacccaattg atacattccc acctcaaacg
    13441 ccacccgggt acgggtggag cttttctgaa agatcatccc caacgtcttg ccggccagaa
    13501 tgggatgagg ggttccggcc ttttgttctt ttttcagctg caaaccggtc tccaaaatga
    13561 gggtgatttc ttccgcgctg aaatcatgca gactaataaa gtctctgccc tttagtgctt
    13621 gactgacttc catgacctcc atcctcctac aaacgattta tccttttttg ttccattaca
    13681 ctttccattc ccgcaatgat tttttaaaga tggctaccgc ctgatttatt tcggtttcat
    13741 taatgttaag cggggggaca aaacgcatga cattggcccc tgcccccaac aacaacaagc
    13801 cgttgtgcat gcaaatcgta accaaatcct tgatcggggt gttgaattct gcccccagca
    13861 tgagtccccg tccgcggaca gtaaccattc tttcgtcctg gattgattgc agggattctt
    13921 tgagagatct gcctgccttg tttacattat ccaaaaaagc cggcgcagat acaagctcaa
    13981 ctacacggtc ggccaccgca gtagccaaag gattgccgcc aaaggtggaa gcatggtcgc
    14041 cgggggcaaa gccgccggca gccttttcac tggccagcat ggcgccaatg ggaaacccgc
    14101 cgcccaagcc tttggccatg gtcacaatgt caggcttcac ctgataattc tgataagcca
    14161 taaagtttcc gctgcgtcct accccgcact ggacttcgtc aaatatcagt aaaattcctt
    14221 cccggtcaca aatgcgacgc agactgttta aaaagactgt atcggcagga tggactccgc
    14281 cttctccctg aatgggttcg atcatgatgg cagcggtatc gtcgctgatc agtttttcca
    14341 ctgaggccag gttgttaaaa tcggcataga caaacccgct gggcaggggg gcaaatcctt
    14401 cctgatactt tttttgtccg gtggcggtca gggtggccaa ggtccggcca tgaaaagact
    14461 cattgaaaac aatgatttgg gtgcggcctg actcttgttg gcgatagaag tatttgcggg
    14521 ccagcttgat ggctgcttca ttggcctccg caccgctgtt gcagaaaaaa gctttatcca
    14581 gaccagagat gctgaccagt ttttccgcca gtcttacctg aggttcgatc caataaaggt
    14641 tggagacatg ccaaagcgat gctgcctgct ggcggatgac ctgctgcaat tcatcatggc
    14701 agtgaccaag ggcacacaca gcaatcccgc cgatgaaatc caaatattgt ttgccgtctg
    14761 catcccagac gtaggaacct ttgcctttga ccggagcgat gggaaaacgc ccgtaggtgt
    14821 tcatgactac tttctggccc cgctctgcga tatctttgtt tgtcatattc gtatcctccc
    14881 ggtaatcaat cctattcttg ttaacccgat ttttcatcta ttttgtgact aatgcaactg
    14941 tttattcatt gacaaccatg gtaccgattc cctcattagt gaaaatctcc aacaaaatgg
    15001 agtggggtac gcgtccgtcg acgatatggg tacgggcaac accgccggtg accgcttcca
    15061 cacagcattg gaccttggga atcatgcctc cggcaatgat gccctcttca acatagtcag
    15121 gcacctgact gacctttagt accgaaatta gtgatctgct gtcgttttta tcccgtaata
    15181 gtccgggaac atcggtcaac agtaccagtt tatcggcttt cataccaaca gcaatgctgg
    15241 cagcaaccaa atctgcattg atattataac tttgctgctc atcatcgata ccgatcggag
    15301 caatgaccgg gatataacca ttctcaatca aggtttcgat aatctgcgga ttaaccgtat
    15361 ccacttctcc cacaaagccc agtttgccgc tgggatcaac tttgcgggct ttgatgagtc
    15421 ctccatcctt gccggatatg ccgactgcct tgcccccgga ggcattcaag ccggatacga
    15481 tttctttgtt gaccttgccg ccgagtacca tttccactac ctccatcgtt tcccggtcgg
    15541 tgacccgcaa accgttgacg aactcggtat ccatgtttaa ccgccccagc atgcctgtga
    15601 tatcaggtcc gccgccatga acgacaatgg gatgcatgcc aacgtatttc atcaagacaa
    15661 tatcctgcat gactttcatt ttcagatcac attcagtcat agctgcaccg ccatatttta
    15721 ttaccactcg tttgccgtaa aagtctttga tataaggcag ggcttcaacc agcacctccg
    15781 ctttttccat tgctgata
//
LOCUS       DAOTHZ010000052        15388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4642_length_15388_cov_7.210200, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000052 DAOTHZ010000000
VERSION     DAOTHZ010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 15388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15388
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4642_length_15388_cov_7.210200"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="P5549_07125"
     CDS             <1..1170
                     /locus_tag="P5549_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12880.1"
                     /translation="PSYQIAVIPIVPTTAAIDNIPTFRDSFGKLYDAFDQIQNGVGDP
                     AKDATLLFGLNKVKGGLGTVSDGIGTVLDNLKTIRIGVSNPSFNTATYDATKGMDASG
                     NKPGVKEAVTMSKTALDTQMLAAMEGQKKVLTVMQDTMGKPGAEPVTPSLSTSLYNDI
                     DFLKGLVKGTPAEQVITQALAPKILAMGTNLGVFRDGGTLISSSGSVPFPASVTAVES
                     GIQQVSSGLGKANNGLSFMAIGLGQLDENGNPKPVMVNGKPGSLLYALDYIQAGVDGQ
                     LVPGVDQLSGGAAKIGDGSGQAKTAVAGGLDTLEAVPAIVSALQDNLTQNDTFLGKPE
                     GSGASVTYVYQTVAISSSASAMTYGLGAIALALLILFIIGRPKKAPLAAPVEQEM"
     gene            1193..2005
                     /locus_tag="P5549_07130"
     CDS             1193..2005
                     /locus_tag="P5549_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRW12881.1"
                     /translation="MKKKFAVLLCLAMFMTIFAAPALAADPTVSVVIDGQAVTFADQA
                     PIIDNSRTLVPLRAIFEAMGATVSWDDATKTATGVKGDITVVVAIGSVSPTINGVVKT
                     IDVPAKIVNSRTLAPLRFVCEAFGGTVSWDGATYTASVVSAGGAAPAPAPEPAPAPAP
                     AAATALSSASGTISKSAPADVPITITWGSATKVSKMLAGSPAFGLTYDPHEGVDYTVA
                     DNGDGTGVFVIKKELSNQLPVPLNMVPTGAEMAITIQFDNGTNLDYSLKVVD"
     gene            2223..3263
                     /locus_tag="P5549_07135"
     CDS             2223..3263
                     /locus_tag="P5549_07135"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRW12882.1"
                     /translation="MFEYIGNMHIHSRYSDGSATIGKIRSDARRAGLDFFIVTDHFTL
                     QAAGQEGYQDGVLMLVGMEANEAHNHYLALDIAQVVPNHTDHPQKVIDAVNQQGGIGI
                     IAHPLEYASNFLNEGKVYNWIDWSVEGFQGIEIWNLLSQWKGSIGNFCQAVYYALINP
                     QAALVGPYPEIMRRFDDYQQQGRRIVAFGGADAHAPGITLGPFHFKIISYLFSFRSIN
                     MHILLEQPLSGELSADKQLIYQAFRQGACWVGNDYYQTSKGFSLSVSNGQQTWLMGND
                     VPWQEALQCKVCTPARARIKLYRNGKLAAETEGTELSLREVSCGVYRAEACLLHRGKY
                     RPWIFSNSIWVI"
     gene            3423..3854
                     /gene="mraZ"
                     /locus_tag="P5549_07140"
     CDS             3423..3854
                     /gene="mraZ"
                     /locus_tag="P5549_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRW12883.1"
                     /translation="MFLGEYQHSLDIKARFIVPARFREELGTRFVATKGLDNCVFIYP
                     LTEWKSIEEKIRSLPFTRADVRSFARFFFSGAAELEPDKQGRVVLPQNLREYAQIDKD
                     LVIIGVGPRIEIWASPIWEAYNQTAASSYESYAESLVDLGL"
     gene            3867..4784
                     /gene="rsmH"
                     /locus_tag="P5549_07145"
     CDS             3867..4784
                     /gene="rsmH"
                     /locus_tag="P5549_07145"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRW12884.1"
                     /translation="MHQAVLLEETIGYLLNDPHGTYVDCTLGGGGHLRCLTEKLEADA
                     WVIGIDKDLNILEQTRKTLDFPRMKLIHGDFRKLDEILASLKIDKVHGIMMDLGVSSF
                     QLDEADRGFSFHEDAALDMRMDQHQILNARDVVNEYSQEDLTHILFQYGEEKYARSIS
                     RAIVNRRQQSPIEGTMELVEIIQNAVPASYRREKHPARKTFQALRIEVNGELDALQEV
                     LPKAVDRLFPGGRLCVITFHSLEDRIVKKFLQDKARECICPPGLPICVCEHHAELEIL
                     SRKPIIASQAECLSNPRSRSAKLRAARKL"
     gene            4812..5288
                     /locus_tag="P5549_07150"
     CDS             4812..5288
                     /locus_tag="P5549_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRW12885.1"
                     /translation="MQQVAYKYADSWQEKVELPVIEPRIRKVVKTKSNQHRKIMVKAG
                     LFMFGYAVLLVFLCAKSASLGYDIEGLNRDVSKLTTANHRLEYQIASYSSLSRIEKIA
                     VSELGMQRAELQNSIAVKVEPKPVQVAVREQETTNLSERSLYKIYTSLSHLAQNSR"
     gene            5316..7436
                     /locus_tag="P5549_07155"
     CDS             5316..7436
                     /locus_tag="P5549_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HRW12886.1"
                     /translation="MQTAGLLVRKRILALFLLFIVLFLALVTRIFWVQFVRGAELSAK
                     ATQNRMRDVPVEAKRGIITDCNGRELAISISTDSVYAIPAEINKSKKSEWVAQQLAEI
                     LDMNEADLLKRVTRASSFEWVKRQISPEAAKKIRELELPGIGLTEESRRFYPRGSLAS
                     HILGISGTDNTGLEGIDNYYNGLVGGKKGRIIIEHDAAGANIPEAMHKYIAPVDGANL
                     TLTLDETIQYITERELDKAFKEFKAKRAAAIVMDPKTGAILAMSSRPTFDPNNYNQYP
                     DDNRRNFAINDAYEPGSTMKITTAAMALQEAVVNDSSRFFCPGHVKVGKNSIGCANNK
                     AHGDQTFAQIVENSCNVGFVQVGLELGMEKYFQYLHGFGFGRKTGIDLPGEAEGILVP
                     ESRATQLDLATMAMGQANAITPIQLVTAVSAVANGGKLMKPYLLKEAVDQEGKVIKKN
                     EPQMLKRIISEESAHKLCMILEGEVTQGTGKNAFVEGYRVGGKTGTAQKIKPGGGYLE
                     GEYVSSFIGLAPADDPRLVCLIVVDAPQGYPYYGGTVAAPGVREILKDSLRYMNVPLR
                     GETAPAETEQSLADTVLVPDLVNLPLSEALSSLNNRSLNAKVEGEGDLVWQQAPKART
                     RVARGSTIILNLSPYSGENASAEVTVPDLEGKSMKEVAKILSDLGLHLSPQGYGISYE
                     QSPQAGKIISTGSTIKVKFKPLQD"
     gene            7518..8987
                     /locus_tag="P5549_07160"
     CDS             7518..8987
                     /locus_tag="P5549_07160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRW12887.1"
                     /translation="MHIEKLLQGLDYKLVKGPNDADVQGLCYDSRKAAEGFLFICIPG
                     LKTDGHQFASQAVANGAKVILAEKEIELPDGITLLLTSDCRHALPIVSANFFGRPSRE
                     VKVIGVTGTNGKTTTTHLIKSILEEAGHKTAIIGTLYAKIDETEKVLGHTTPESLDIE
                     AFIAMARQEGARYVVMEVSSHALDLGRVEQIFFDAAVYTNLTQDHLDYHQDMEHYKQS
                     KRKLFQQLPAGTGCFGVVNADDPYAQDFVKAAPRTFTYAVDQPADVRAVEVDSNLKGS
                     SFTVITSSGQFAINMKLIGMFSVYNALAAICYALHEGIESATIKSALEKVAGVAGRFE
                     QVDCGQDFTVVVDYAHTPDGLENILRTGRQIVQNRLITVFGCGGDRDRTKRPLMGRIA
                     AQWSDFSIVTSDNPRSEEPEAIIDEIVPGLDTVENSHYAKIVDRRDAIEHALRLAKPG
                     DLVIIAGKGHETYQLVKDQVLDFDDRKVAREILKGLLEQ"
     gene            8984..10360
                     /gene="murF"
                     /locus_tag="P5549_07165"
     CDS             8984..10360
                     /gene="murF"
                     /locus_tag="P5549_07165"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRW12888.1"
                     /translation="MNLQLDFIVEAVKGRLLSGNCAVVHEVSTDSRKVGPGSLFVALK
                     GEHFDGHDFVVKAIEKGATAVLVSRRDLALPSGCKAAVILVDDTLAAMQNLAACYRQR
                     FTLPTIAITGSVGKTTTKDILADLLARRYRTLKTQGNYNNEIGLPMTLFHLQPEHQAA
                     VLEMGMRASGEIHHLASILNPRYAIITNVEAVHLETMGTLENIAKAKCEVLEFIKNDG
                     FALLNGDNQLLLDTAGRFSCRKYLFGCKDGNHIQIKSLLHDGEGIQVRLQLFECEEDF
                     TLPVPVSQLAYNLAAAVGMAWLMGVSCEEMRAALKDFQPGGNRMHMTELSGGGVLIDD
                     SYNANPISVMAALEACQKISRGRRKIAILGDMLELGDYAREGHLKAGRHAVQVAMDLL
                     VTIGPLSQYYREGAIAQGMPENRTRHFADRQTALDWLQANVSPADVILVKASRGMHLD
                     ELARELFR"
     gene            10384..11358
                     /gene="mraY"
                     /locus_tag="P5549_07170"
     CDS             10384..11358
                     /gene="mraY"
                     /locus_tag="P5549_07170"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRW12889.1"
                     /translation="MPNHFTNALIAAGIAFIVCLAMGPFMIPFLRRLKVGQSIRDDGP
                     QTHLSKAGTPTMGGIIMVTAVMIASFLMAGASKQVLVSVLIMLAVGFIGFRDDYIKVV
                     LKRSLGLRARDKLLLQTLIGFVMGLLLLFYLDRGTQLIIPFTSVTVELGYLYIPFIML
                     VLIATSNGVNLTDGLDGLAAGVTVPVALAYIVISIMQGNFNMMIFGAALIGACLGFLV
                     FNHHPAKVFMGDTGSMAIGGAITALAAVNRSEIALLIIGGVFVVESLSDIIQVASFKT
                     TGRRVFRMAPLHHHFELKGWPERKVVWMFWLLSIGFALLGIWGYKNIG"
     gene            11373..12740
                     /gene="murD"
                     /locus_tag="P5549_07175"
     CDS             11373..12740
                     /gene="murD"
                     /locus_tag="P5549_07175"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRW12890.1"
                     /translation="MDFTDKNVMVIGLARSGLAALKALHKRGARLTAYDAKAAADLGG
                     CVAELQAMDVTLYTGEYPPVDADLDLLVASPGVPLEIEPIQSALRQQIEVISELELAY
                     RIKSEQLEMLAVTGTNGKTTTTTLLEKILLAAGRPAAAGGNIGVALCSLVEEMQDGYI
                     SVEASSFQLETASTFRPHIAAILNITPDHLDRHKTMEGYMAAKAKVFARQTKDDFLVL
                     NYDDEQVKKMGDQAGSRVIFFSQEQSLEEGVFVAEHFIRIKWQGEIHDLMPVAELRLR
                     GRHNLENALCATAMAWADGVSLESIAQVLRTFNGVRHRLEEVACHNDILYINDSKGTN
                     PDSTIKALCAFDRPIVLIAGGRPKGGSYQQVAREISQRVRELVLLGEAREMIKSEVME
                     SGFRNIHEVEDFPAAVYKAHQLARPGDVVLLSPACASWDMFPSYEHRGDQFCQIIDSI
                     IKQQS"
     gene            12798..13898
                     /gene="ftsW"
                     /locus_tag="P5549_07180"
     CDS             12798..13898
                     /gene="ftsW"
                     /locus_tag="P5549_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HRW12891.1"
                     /translation="MRLKKGPPDFVLFITTLALIGIGLIMVFSSSSVTANVNYDDAYY
                     FFKKQLMWAFIGLVAMIIVMKLNFLRLRGFAVPLLVLALVLLILVVTPLGMEVKGSSR
                     WFLLGPVRFSPSEFAKLAMVLFLAKSMEANINKMGSFTQGVLPYIFMLALGCGLIMLQ
                     PDLGTAFVLAGTMFFMMMVAGAKITHLGLLSLPAIAAVSAAIYFEPYRMQRLIAFLDP
                     WKYANDEGFQTIQSLYALGSGGLFGMGLGRSRQKFFYLPEQHTDFIFAILGEELGFIG
                     VTLVLGLFLLFAWRGFKIAINAPDTFSSILASGITILIVFQAVINIGVVSGALPVTGI
                     TLPFISYGGTSLLFSMIGVGLLLNISRYSTNK"
     gene            13909..15015
                     /gene="murG"
                     /locus_tag="P5549_07185"
     CDS             13909..15015
                     /gene="murG"
                     /locus_tag="P5549_07185"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRW12892.1"
                     /translation="MKIIISGGGTGGHIYPALAIASELQQRIPEVQLLYVGTPAGMES
                     RIVPAAGLTYAALDVSGIDRTSMLKASKSMIKFPNSFFQARNIIKEFQPDMIVGTGGY
                     VSFPIVLAGTFMGVKTIIHEQNAYPGLANRNLAKRADRVLLTFPEARQYLDTDSIRVT
                     GLPVRKEIKPMDKQKARQRLGLDSRLFTILAFGGSLGAQTINNAVLDFIDRSRSQDLQ
                     LLWITGEAAYEQMKEELERRFDRQTLRPQIKLFPYLNQMEQAMGAADLAVCRAGASTV
                     SELAMIGLPAILVPFPFAADNHQEKNARALVNKNAAVMVIDEFFDGTEMVKKVQDLAR
                     QPEKLKAMGQNMQKEAKPAALKEITDEILQLLQG"
     gene            15148..>15388
                     /locus_tag="P5549_07190"
     CDS             15148..>15388
                     /locus_tag="P5549_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRW12893.1"
                     /translation="MTANQQEWIHMVGIAGAGMSGIARVLKEQGSKVSGSDLQKNGVS
                     SRLEEIGIQIYEGHHSSNIQEGVDLLVISSAVPPEN"
BASE COUNT         3949 a         3467 c         4104 g         3868 t
ORIGIN      
        1 cccagttatc aaatcgcggt aatcccgatc gtacctacta ctgcagcgat tgacaatata
       61 ccgactttcc gtgacagctt tggcaaactg tatgatgcgt ttgaccaaat ccaaaacggg
      121 gtaggggatc ctgccaaaga cgccaccttg ttgtttggac tgaacaaagt caaaggcgga
      181 ttgggtacgg tcagcgatgg tatcggcacg gtactggata atttaaaaac catccgtatt
      241 ggagtttcca atccaagttt taacacagct acctatgatg ctaccaaagg tatggacgcc
      301 agcggcaaca aacccggcgt caaagaggcc gtaaccatga gcaagaccgc tctggacacg
      361 cagatgttgg cggccatgga aggacagaag aaagttctga ccgttatgca ggatacgatg
      421 ggcaagcccg gtgctgaacc ggtcactcca agtctttcta ccagcctgta taacgatatt
      481 gatttcctga aaggactggt caaaggaacc ccggcagaac aagttatcac gcaggccctg
      541 gcgcctaaaa tcttagccat gggaaccaat ctgggcgtgt ttagagatgg cggaaccctt
      601 atttcatcca gcggcagcgt tcccttcccg gccagtgtca ctgctgtcga aagcggaatt
      661 caacaggtat catccggtct gggcaaagcc aacaacggat tgagctttat ggccatcgga
      721 ctgggtcagc tggacgaaaa cggcaacccc aaaccggtca tggtcaacgg caaacccggc
      781 agccttcttt atgcattgga ttatatccag gctggggttg atggccagct ggttcccggt
      841 gtagaccagc tcagtggcgg tgctgccaaa attggtgacg gttccggcca ggccaagaca
      901 gctgttgccg gcggactgga taccctggag gccgttcctg ccattgtctc agctttacag
      961 gacaacctga cgcaaaatga cactttcctg ggcaaaccgg aaggctcagg cgcttctgtt
     1021 acctatgtgt atcagaccgt tgccatcagt tcttcggctt ctgccatgac ctacggattg
     1081 ggtgcaattg ctctggcgct gctgatcctg tttattatcg gcagaccgaa aaaagctccg
     1141 ttggctgcac ctgttgagca agaaatgtaa tgaatcgtta ggaggataaa atatgaaaaa
     1201 gaaatttgca gtattgctgt gtttggccat gttcatgacc atctttgcag caccggcttt
     1261 ggcagctgat cctaccgtca gcgtagtgat tgacggccag gcagtaactt ttgctgatca
     1321 agcaccgatt atcgacaata gcagaaccct ggttccgctg agagctattt ttgaagccat
     1381 gggcgctacc gttagctggg atgatgctac aaaaaccgct actggcgtca agggtgacat
     1441 caccgtagtt gtggcgattg gctcggtatc tccgaccatc aacggcgtgg ttaaaaccat
     1501 tgacgttccc gcgaaaatcg tcaacagcag aaccctcgct ccgctgcgct ttgtttgtga
     1561 agcatttggc ggcactgttt cctgggatgg agcaacctat actgcttccg tagtttctgc
     1621 tggtggcgct gcccctgcac ctgctccgga acctgcaccg gcacctgctc cggcagctgc
     1681 taccgcgctg tcatcagctt ccggaacgat cagcaagagt gcacctgctg atgttccgat
     1741 taccatcacc tggggttcag ccaccaaggt gagcaagatg ctggccggat cacctgcctt
     1801 tggtcttact tatgatcccc atgaaggggt tgactacacg gttgccgaca atggcgacgg
     1861 aaccggcgtt ttcgtaatta aaaaagaact ttccaatcaa ctgcccgtac cgctgaacat
     1921 ggtaccaacc ggtgctgaaa tggcgatcac cattcagttc gataacggca ccaatcttga
     1981 ttacagcttg aaagttgttg actaatcaca cggattgact gattaaaaaa gatgaccggg
     2041 ctatgcccgg tcatcttttt tgctggcatt cccccaaaaa tgcttttgtt aagtcttatt
     2101 ttcttgcatt aaatataggg atattgtttt gagcaatcca cccgtctgtt ttataatatt
     2161 tgtaaatgca tatgatgcta aaaagaagcc atcgtcaaat cataccggca gggggaagag
     2221 gaatgtttga atatatcggc aatatgcata ttcattcccg ttattcggac ggcagtgcga
     2281 ccatagggaa gatacgcagc gatgcccgcc gagctggttt ggattttttc atcgtcaccg
     2341 atcattttac gttgcaggcg gccgggcagg aaggctacca ggatggcgtg ctgatgttgg
     2401 tggggatgga ggccaacgaa gcccacaacc attacctggc cttggatatt gcgcaagtcg
     2461 tgcccaacca taccgaccac ccccagaagg ttatcgatgc ggtcaatcag caagggggaa
     2521 ttggcatcat tgcccatccg ctggaatatg cgtccaactt cttgaatgaa ggcaaggtct
     2581 ataattggat cgattggagc gtggaagggt ttcagggaat tgaaatttgg aatctgttgt
     2641 cacagtggaa agggagtatc ggaaatttct gccaggcggt atattatgcc ctcattaacc
     2701 cgcaggcagc cttggtcgga ccttacccgg aaatcatgcg cagatttgat gactatcagc
     2761 aacagggccg aaggattgta gcttttggag gagcggatgc tcatgctccg gggatcactc
     2821 tgggaccgtt ccactttaaa atcatatctt atttattcag ttttcgttcc atcaatatgc
     2881 atattctgct tgaacaacct ttaagcggtg aactgtctgc cgacaagcaa ttgatttatc
     2941 aggcatttcg ccaaggagca tgttgggttg gcaatgacta ttaccaaacc agcaaaggat
     3001 tttctttatc tgtgagcaac ggacagcaaa cctggctcat gggaaacgat gtgccctggc
     3061 aagaagcctt gcaatgcaag gtgtgtactc ccgcccgtgc ccggataaag ttgtatcgaa
     3121 atggaaaact ggccgcggaa acagagggaa cggagctttc cctgcgagaa gtgtcttgtg
     3181 gagtgtatcg agccgaagct tgtttgttgc atagaggcaa gtaccgaccc tggatatttt
     3241 ccaattcaat ctgggtgatt taaaaagagc gcaataaaag aagcgctttt tttaaaaaaa
     3301 atattgttca aatattcaca aagaaggata tatgaaataa tactagaagt actaaaaaag
     3361 tggagagaag tggagagaag tggatgaaaa tcccataaaa aaccccacaa ggagaagaac
     3421 gaatgttctt aggcgaatat cagcattcat tagatataaa agccaggttt attgttccag
     3481 cgagattccg cgaagaactg ggaacgcggt tcgtggcgac caaaggcctg gataattgtg
     3541 tttttattta tcccttgacg gagtggaaaa gcatcgaaga aaaaattcgt tcccttcctt
     3601 ttaccagagc tgatgtccgt tcttttgccc gttttttctt ctcgggagct gctgagctgg
     3661 aaccggacaa gcagggaaga gttgttcttc cgcaaaactt aagagaatat gcgcagatcg
     3721 acaaggatct cgtcattatc ggcgtgggtc cccggattga gatttgggcg agcccgattt
     3781 gggaagccta taaccaaacc gcggcttctt cgtatgaatc atatgcagag agtctggtgg
     3841 atttgggatt gtaagggtga tgaagattgc atcaggccgt attgctggaa gagacgattg
     3901 gttatctgct caatgacccc catggcacct atgttgactg tacactgggt ggcggagggc
     3961 atctgcgttg cctgacagaa aagctggaag cggatgcttg ggtgattggc attgacaaag
     4021 atttaaatat tctcgaacag acccgtaaaa cattggattt tccccggatg aaactgatac
     4081 atggagattt tcgcaaactt gatgaaatac tggccagtct gaagattgac aaagtccatg
     4141 gaatcatgat ggatctgggg gtttcttctt ttcaacttga tgaggccgac cgcggtttca
     4201 gttttcatga agatgccgcc ctggatatgc gcatggatca gcaccagatt cttaatgcac
     4261 gcgacgttgt caatgaatac agccaggagg atctgacaca tatattgttc caatacggag
     4321 aagaaaaata tgcccgttcc ataagccggg cgatcgtaaa tcgacgtcag caatctccca
     4381 ttgagggcac catggaactg gtggaaatca ttcagaacgc tgtgcccgcc agctatcgcc
     4441 gggaaaaaca tccggcccgg aaaacgtttc aagctttacg gattgaagtc aatggagaac
     4501 tggatgcctt gcaggaggtc ctgcccaagg cagttgaccg tctttttccc ggaggaagat
     4561 tatgtgttat tacatttcat tccctggaag atagaatcgt aaaaaaattt ctgcaggata
     4621 aagcgcgtga atgcatttgt cctccaggat tacctatatg tgtatgtgaa catcatgccg
     4681 aattggagat tttaagtcgt aaacctataa tagccagcca ggcagaatgt ttatccaatc
     4741 cccgttctcg aagtgccaaa cttcgagcag cccgcaagtt atagttctaa ataataggga
     4801 ggtttaataa gatgcaacag gtagcttaca aatatgcgga ttcatggcag gaaaaagtcg
     4861 aattaccggt tattgaacct cggattagaa aagtagttaa aaccaaatcc aatcaacatc
     4921 gcaagataat ggttaaagcc ggactgttta tgtttggcta tgcagtattg ctggtttttt
     4981 tatgcgccaa aagtgccagt ctggggtatg atatcgaggg gttgaatcgg gatgtcagca
     5041 aactgacgac agccaatcat cgcctggagt atcagattgc cagttacagt tcactgtccc
     5101 ggattgagaa gattgccgtc agcgagttgg gtatgcaaag agcagaactg caaaactcaa
     5161 ttgccgtgaa agtagaaccg aaaccggtac aggtagctgt tcgtgaacag gagaccacca
     5221 acctgagtga aaggtcttta tataaaatat ataccagctt atctcatctg gctcaaaaca
     5281 gtcgctagaa aacgcttaaa tgaggtggac tgaatatgca aactgctggt ttgctggtaa
     5341 gaaagcgtat cttggcgctt tttttattat ttattgtatt atttctggct ctggtaacga
     5401 gaattttctg ggtccagttt gtcagaggtg cggagctttc tgccaaggca acccaaaacc
     5461 gtatgcgcga cgtaccagtg gaagccaaac gagggataat aacggactgc aatggtcgtg
     5521 aattggcgat atccatcagt actgattcgg tgtatgccat tccggcagag ataaacaaat
     5581 ctaaaaaatc tgaatgggtt gcccaacagc tggctgaaat cctggatatg aatgaagctg
     5641 atttgttaaa gcgagttact cgtgccagtt cctttgaatg ggtcaaacgg cagattagtc
     5701 cggaggctgc gaaaaaaatt agagagcttg aactgccggg gatcggactg accgaagaga
     5761 gtcgtcgttt ttatccccgc ggcagtctgg ccagtcacat cctggggata tccgggactg
     5821 acaataccgg attagaagga attgacaatt attacaatgg cttggtgggc ggcaaaaagg
     5881 gccgtatcat tatcgaacat gacgcggccg gagccaatat cccggaagcc atgcacaagt
     5941 atattgcgcc cgtggacggt gccaatctga cgcttacgtt ggatgaaacc attcaatata
     6001 ttaccgagcg tgaacttgac aaagccttta aagaatttaa agccaaacgg gccgcggcaa
     6061 tcgtaatgga tcccaaaacc ggtgccattc tggcgatgtc atcacgccca acctttgatc
     6121 ccaacaatta caaccaatat ccggatgaca atcgccgcaa ttttgccatc aatgatgcct
     6181 atgaaccggg ttccaccatg aaaatcacga ctgcagccat ggcactgcag gaagcggtgg
     6241 ttaatgacag cagtcgtttc ttttgtcctg gtcatgtgaa agtcggaaaa aacagcattg
     6301 gctgtgccaa taacaaagcc catggagatc agacctttgc ccaaattgtt gagaattcat
     6361 gcaatgtagg gtttgttcaa gtcggcctgg aactgggaat ggagaaatac tttcagtatc
     6421 tccatggatt tggatttggg cgcaaaaccg gcattgatct gcctggagaa gccgagggaa
     6481 tccttgttcc ggagagccgg gccacgcaat tggatttggc gaccatggcg atggggcagg
     6541 ccaatgcaat caccccgatt cagctggtta cggccgtatc agctgtggca aacgggggaa
     6601 aattaatgaa accctatctg ttgaaagagg cggttgatca ggagggtaaa gtaatcaaga
     6661 agaacgaacc gcagatgctc aaacgcatca tatccgaaga aagtgcccac aagttgtgca
     6721 tgattctgga aggcgaagta acccagggaa ccggaaaaaa tgctttcgtt gaaggctacc
     6781 gggtaggcgg caagacaggt accgctcaaa aaattaaacc cggcgggggt tacctggagg
     6841 gtgaatatgt atcttctttt atcgggcttg ccccggctga tgatccgcgt ctggtttgtc
     6901 tcattgttgt agatgctccg cagggctacc catattatgg aggaacagtt gccgctccag
     6961 gcgtacggga gattttaaaa gattccctgc gttatatgaa tgttcccctg cggggggaaa
     7021 ccgctccggc ggaaacagag caaagtttgg cggatactgt tctggttccg gatctggtta
     7081 atctgccgtt gtccgaagcg ttaagcagtc tgaacaaccg gagcttgaat gccaaagtgg
     7141 aaggggaagg cgatttggtt tggcagcagg cccccaaagc ccgcacccgg gtcgcccggg
     7201 gttccactat tattctcaat ctttctcctt actccgggga aaatgccagt gccgaggtaa
     7261 ctgttcctga tttggaggga aaatccatga aggaagtggc caaaatactg tctgatttgg
     7321 gtttgcatct ttcgccccag ggatacggca ttagttatga acagtcaccc caggccggga
     7381 agataatatc caccggttca accattaaag taaagtttaa accgctgcag gattgatctg
     7441 ctgtacaggc aatggcctgc aaagcaaacc tggcacatgc agctgcaaag atagtttatt
     7501 gaaaggagga gttgctggtg catatcgaaa agcttttaca gggtttggat tacaaactgg
     7561 tcaagggacc aaatgatgca gatgtgcaag gattgtgcta cgattcccga aaagctgccg
     7621 aaggctttct gttcatctgc attcccggtt tgaaaaccga tggacatcaa ttcgcatccc
     7681 aggcggtcgc caacggtgcc aaagtcattc tggcggaaaa ggagattgaa ctgccggatg
     7741 ggattaccct ccttttaact tcggattgcc gccatgctct gccgattgtc agcgccaact
     7801 tctttggccg gccttcccgc gaggtaaagg taattggggt gaccggcacc aacggcaaga
     7861 caaccacgac ccatttgatc aagtccattc tggaagaggc cgggcacaag accgcgatta
     7921 tcggcacgct gtatgccaaa attgacgaaa cggagaaagt attgggacac accacaccgg
     7981 agtcgctcga tattgaagcg tttatcgcca tggccagaca ggaaggtgcc cgctacgtag
     8041 tgatggaggt ctcctcgcac gcgctggatc ttggccgggt tgagcaaatc ttttttgacg
     8101 cggcggtgta taccaatttg acccaggatc atttggatta tcaccaggat atggaacatt
     8161 acaagcaatc aaagcgcaaa ctttttcagc aacttccagc tgggacaggc tgcttcgggg
     8221 tagtcaatgc cgatgatccc tatgcacaag atttcgtgaa ggcggctccc cggactttta
     8281 cctacgccgt cgaccagcct gctgatgtcc gtgcggttga ggttgattcc aatctcaaag
     8341 gcagcagctt tactgtcatt acctcatccg gacagtttgc gattaacatg aagctgattg
     8401 gcatgttcag cgtatataat gcattggcgg ccatttgtta tgccttgcat gaggggattg
     8461 aatctgcgac aataaaatct gccttggaaa aagtagccgg cgtggcagga cgatttgaac
     8521 aagtggactg cgggcaggat tttaccgtgg ttgtagatta tgcccatacg cctgacgggt
     8581 tggaaaatat attgcgtacc ggccggcaaa ttgttcaaaa ccgtctaatc acggtttttg
     8641 gctgcggcgg agatcgggac cgcaccaaac ggcctctcat gggaagaatt gctgcccagt
     8701 ggagtgattt ttcgatagta acttcagata atccccgcag tgaggagccg gaagccatta
     8761 ttgatgagat tgttccagga ttggacaccg tggaaaattc ccattatgcc aagattgtgg
     8821 atcggcgtga tgccattgaa cacgctctgc gtctggccaa acccggtgat ttggtaatca
     8881 ttgccggcaa gggacatgag acgtatcaac tggtcaagga tcaggtcctg gattttgatg
     8941 atcgcaaggt ggcccgggaa atattgaaag gattactgga acaatgaacc tgcagttgga
     9001 tttcatcgtc gaggcggtca aaggaagact tttatcaggg aattgtgctg tagtgcatga
     9061 agtaagcact gattcccgta aagttggccc agggagtctg tttgtggctt taaagggaga
     9121 acactttgac ggccatgact tcgttgtcaa ggcgatcgaa aagggcgcga cagcggtact
     9181 ggtttccaga agggacttgg cgttgccttc cggatgcaag gcggctgtca ttttggttga
     9241 cgatacgctg gcagccatgc aaaacctggc cgcctgttat cggcagaggt tcaccctgcc
     9301 gacgatcgca atcactggaa gcgtcggcaa aacaaccacc aaagatatcc tggccgattt
     9361 actcgccagg cgctatagaa cactgaaaac ccaggggaat tataataatg aaatcggctt
     9421 gcccatgacg cttttccatt tacagcccga gcaccaggct gccgtcctgg agatgggcat
     9481 gcgtgcttcg ggcgagattc atcatctggc ttcaattttg aatccacgtt atgcgattat
     9541 caccaatgtt gaagctgtcc atctggaaac catgggaacc ttggagaata ttgccaaggc
     9601 caaatgtgaa gtattggaat ttataaaaaa tgacggtttt gctttactaa acggagacaa
     9661 tcaactgctc ttggacacgg ccggtcgatt ttcctgcagg aaatatcttt ttggctgcaa
     9721 ggatggcaat catattcaaa taaaatcgct tcttcatgat ggcgaaggca ttcaggtgcg
     9781 gttgcagttg tttgagtgtg aagaggattt taccttgcct gtgccggtca gccagcttgc
     9841 ctataacctg gctgcggcag tcggaatggc ttggctaatg ggcgtaagtt gtgaagagat
     9901 gcgggctgcc ttaaaagact ttcagcctgg cggaaatcgc atgcatatga cggaattgag
     9961 cggaggcggg gtgctgatcg atgacagtta caatgccaac ccgatttcgg tcatggctgc
    10021 cctggaagca tgtcagaaaa tcagccgggg ccgcagaaag atagccattc tgggagatat
    10081 gctggaattg ggcgattacg caagggaggg tcatttaaaa gccggccgcc atgcggtgca
    10141 agtggcaatg gatctgctgg taacgatcgg tcctctgtct caatactata gggaaggtgc
    10201 aattgcccaa ggcatgccgg aaaatcgtac ccgccatttt gcagaccgac aaacagcgct
    10261 ggattggctc caggccaatg tcagcccggc cgatgtgatt cttgtcaagg cttcccgggg
    10321 aatgcatctg gatgaattgg cccgggaact tttccgttag atttaaccgg gaggataatg
    10381 taaatgccga atcattttac gaatgcattg atcgcagctg gcatcgcttt tatcgtgtgt
    10441 ctggccatgg gcccgtttat gatccctttt ttacgcaggt tgaaggtcgg acaaagcatt
    10501 cgcgatgacg gtccccaaac ccacctcagc aaggccggca cacccaccat gggcggcatc
    10561 attatggtca ccgcggtgat gatcgccagc ttcctgatgg ccggcgccag caaacaagtg
    10621 ctggtttccg tattgatcat gttggccgtt gggtttatcg gatttaggga cgattacatt
    10681 aaagtagttc tgaaacgctc cttggggctg cgtgcccgtg acaaactgct gctgcagact
    10741 ttgatcggat ttgttatggg tttgttgctg ctgttttatc tggatcgcgg cacccagctg
    10801 atcatcccgt ttacttccgt gaccgttgaa ctgggctatc tttatatacc atttataatg
    10861 ttggtcctga tcgccacctc caatggcgtc aatctgaccg atggcctgga tggattggct
    10921 gctggagtta cggttccggt agcgttggct tatatcgtga tttcgatcat gcagggcaat
    10981 tttaatatga tgatttttgg cgcggcactg attggggcct gccttggttt tttggttttc
    11041 aatcatcacc ccgccaaagt gtttatggga gataccggtt ccatggccat cggcggggcg
    11101 ataaccgcgc tggcagccgt aaatcgttcg gaaatagcgc tgttgattat cgggggcgta
    11161 tttgtggtcg aatccctgag tgatattatt caggtagcct cttttaaaac gactgggcgc
    11221 agagtatttc gcatggcacc gctgcatcac cattttgagc tgaagggatg gccggagcgc
    11281 aaagtcgttt ggatgttctg gctgctctcc atcggatttg cgttgctggg gatttggggc
    11341 tataaaaata ttggatagga agtgtgtggc gcttggattt tacagataag aacgtaatgg
    11401 tcatcggcct ggctcgcagt ggcttggcag ctctcaaagc attgcataaa agaggtgccc
    11461 gcttgacagc ttatgacgcc aaggcggcag ccgacctggg tggttgtgtt gcggagctgc
    11521 aagccatgga cgtgacgctt tataccgggg aataccctcc ggtagatgct gacctggatt
    11581 tactggtggc aagtcctgga gtgccgctgg aaatagaacc gattcaatcg gcactgcgtc
    11641 agcaaattga agtgatcagc gagctggaac tggcttaccg aatcaagtcc gagcaattgg
    11701 aaatgctggc agttaccggg accaatggca agaccactac gactacactg ctggagaaaa
    11761 ttctgctggc agcaggacgt ccggcagcgg caggaggcaa tattggagtt gcgctttgtt
    11821 ctttggtgga ggaaatgcag gacggttaca tatcggtgga agcttccagt tttcagctcg
    11881 aaacagcctc aacatttcga cctcatattg ctgcgattct caatattacg ccggatcacc
    11941 ttgatcgtca caaaaccatg gaaggataca tggccgccaa agccaaggtg tttgcccgac
    12001 agacaaaaga tgatttttta gtgttaaatt acgatgatga gcaggtcaaa aaaatggggg
    12061 accaggccgg ttcacgtgtg atttttttct cccaggaaca gtcattggag gaaggcgttt
    12121 ttgttgcgga gcatttcatt cggataaaat ggcagggtga aatccatgat ttgatgccgg
    12181 ttgccgaact gagactgcgc ggcagacaca atctggaaaa cgcactgtgt gcaacggcca
    12241 tggcctgggc ggatggcgtc agtctggaaa gcattgcgca ggtattgcgt acgtttaacg
    12301 gggtcagaca ccgtttggag gaagttgcct gtcataatga tattctatat attaatgatt
    12361 ccaaagggac caatcctgat tctactatca aggcactctg tgcgtttgac cgtccgattg
    12421 tcttgattgc cggcggccgg cccaaaggag gcagttatca acaggttgcc cgggagatca
    12481 gtcagcgtgt cagggaactg gttttgctgg gggaagcccg ggagatgatc aaatcagaag
    12541 taatggaaag cggcttcagg aatatacatg aggtagaaga ttttcccgcc gcggtataca
    12601 aagcccatca actggcccgg cccggcgacg tggttttgct ttctcccgcc tgcgccagtt
    12661 gggatatgtt ccccagttat gaacatcggg gagatcagtt ttgtcaaatc attgattcga
    12721 ttatcaagca gcagtcttag caggaaggcg gtatacttaa gagcaacact gccgtggctg
    12781 gttgcggagg tgagacgatg cggttaaaaa aaggaccgcc ggatttcgtt ttgttcataa
    12841 ccacgctggc tttgattgga atcgggctga tcatggtttt cagttccagt tcagtcacag
    12901 ccaatgtcaa ttatgatgat gcttactatt tctttaagaa gcagctgatg tgggctttca
    12961 ttggcctggt ggcgatgatt attgtcatga agctgaattt tctgcgcttg cggggatttg
    13021 ctgttcccct gctggtgttg gcacttgtcc tcctaatact ggtggtaacc ccactgggga
    13081 tggaggtgaa gggttcatcc cgctggtttt tgctgggacc ggtccgcttc tctccgtctg
    13141 aattcgccaa actggcgatg gttctgtttc tggccaagag tatggaggcc aatatcaaca
    13201 aaatgggatc ctttacccaa ggtgttttgc cgtatatttt tatgctggca cttggctgcg
    13261 ggctgattat gctccaacct gatttgggta cggctttcgt cttggctggt acgatgtttt
    13321 ttatgatgat ggtggcagga gccaaaatca cccatctggg cttgctttct ctgcctgcga
    13381 ttgcggcagt ttccgctgcc atctatttcg aaccgtatcg catgcaaaga ctgatcgcct
    13441 ttcttgatcc atggaaatat gccaacgatg aaggttttca aaccatacag tctttgtatg
    13501 cactggggtc tggtgggttg ttcggaatgg gactggggcg cagccgccag aagttctttt
    13561 atctgcccga acaacatacc gattttattt ttgccattct gggggaagaa ttaggattta
    13621 tcggagttac tctggtattg ggactgtttt tgctttttgc ctggcgaggg tttaaaatag
    13681 ccataaatgc tccggatacc ttttccagta tcctggcttc aggcataacc atcttgattg
    13741 tatttcaggc ggttattaat ataggtgttg tatcgggtgc cttgccggtg accggtatta
    13801 ccctgccttt tatcagttac ggagggacct ccctgctttt ttccatgata ggggtagggt
    13861 tattgctcaa tatttcgcga tacagtacaa acaaatgagg tgtcaggcat gaaaattatc
    13921 atcagcggag gcggaacggg cgggcacata tatccggccc tggccattgc cagtgaactg
    13981 cagcaacgaa tcccggaagt acagctcttg tatgttggga cacctgccgg gatggaaagc
    14041 agaatcgtac cggcagcggg actgacctac gctgctttgg atgtaagcgg tattgaccgt
    14101 acctccatgc tgaaagccag taaatcaatg atcaaatttc ccaacagttt tttccaagcg
    14161 cgcaacatca ttaaagaatt tcagccagac atgattgtgg gaaccggcgg ttatgtgtca
    14221 tttcctatcg tgttggccgg aacctttatg ggagtaaaga ccatcattca cgaacaaaac
    14281 gcctatcccg gactggctaa ccgcaatctg gcaaagaggg ctgaccgggt cttgctgact
    14341 tttcccgaag ccagacaata tctcgatacg gacagcatac gagtgaccgg tttgccggta
    14401 cgaaaagaaa tcaagcccat ggacaagcaa aaagcccgcc agcggttggg tctggattcg
    14461 agactgttta ccattttagc ctttggcggc agcctgggcg cccaaaccat caacaacgct
    14521 gtgcttgatt ttattgatcg cagccgcagc caggatctgc aactgctttg gattaccggc
    14581 gaggctgcct atgagcaaat gaaagaagaa ctggagcggc gctttgatcg tcagacgctc
    14641 cgaccgcaaa taaagctctt cccctatctg aaccaaatgg aacaggccat gggggcggct
    14701 gatctggctg tatgccgggc aggtgccagc accgtcagtg aactggccat gatcggcttg
    14761 ccggccattc tggttccttt tccttttgca gccgacaatc atcaggaaaa aaacgcccgc
    14821 gccctggtga ataaaaacgc agcggttatg gtaatcgatg agttttttga tggaactgaa
    14881 atggttaaaa aagttcagga tttggcaaga caaccggaga aattaaaagc aatggggcaa
    14941 aatatgcaaa aagaagccaa acccgctgcc ttgaaagaaa tcaccgatga gattctgcaa
    15001 ctgctgcagg gataaggcaa agcctaatac caagggattt ctttcattgt cctgcggaca
    15061 agtctttaaa cagaagtgta accacttata ttagcagcgc atactataaa ctgagtcaga
    15121 gatatcgcaa gttaaggaga tgaggaaatg accgccaatc agcaagaatg gattcatatg
    15181 gtgggaatcg ccggcgcagg tatgagcggg atcgccagag tattgaaaga acagggcagc
    15241 aaagtgagtg gttctgatct gcagaagaat ggtgtgagca gccgacttga agaaatcggg
    15301 atccagatct atgaaggcca tcattcctcg aacattcagg agggagttga tttgctggtc
    15361 atatcctcag ccgttccccc cgagaata
//
LOCUS       DAOTHZ010000053        14590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_4955_length_14590_cov_6.870313, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000053 DAOTHZ010000000
VERSION     DAOTHZ010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 14590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14590
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4955_length_14590_cov_6.870313"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="P5549_07195"
     CDS             <1..356
                     /locus_tag="P5549_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRW12894.1"
                     /translation="IMGFVNAPSLFQLVIARCLEAKVDVEAYNRNRELIYHALRDYGY
                     ECIKPEGAFYLFVKAPNDDDVAFCNLAKQKNILVVPGSSFGCAGYFRIAYCVNEETIR
                     KSLPGFETLIKEVKG"
     gene            513..1691
                     /locus_tag="P5549_07200"
     CDS             513..1691
                     /locus_tag="P5549_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRW12895.1"
                     /translation="MKNYKRDLLIALASLLIVVFGAVTVYGAPSVSTVVQAYTGVKIV
                     YNGTTLTSTSEPYIINNTTYVPIRMLMNQFGKDISWDGDTMQVIIKNSAGENAKDKQI
                     EDLKNEVTALNAKIKGLETALGNADTTLRSIDDALDKYQKTAGTAVTTPVTPGISTGS
                     TLSDFQSNLNKYFEDAGDEYFDDDGIEFSINLSGNTVDLVYDITLDFDDADEYDRLNQ
                     ISQSDVKTLLNMVKAKITSEVYGTNYKDAKITGKLSDQDNSSYYVKFNGSSYSYSWDE
                     NDVIDNVIEALEDEYENGGDVYFGDEDIAFEFEINGDEDDLEYEIHMNFDDAYDYDNL
                     REINKTYVRNFLNKVKSTISDEIEDSDYEDADITGYLYDDDNDDYYVKYNGSSYTFSY
                     "
     gene            1780..2544
                     /locus_tag="P5549_07205"
     CDS             1780..2544
                     /locus_tag="P5549_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12896.1"
                     /translation="MTFETRIEDGILIFTFNYPKVNAINMETLQGLDAALDQLQSDDA
                     LKGMVLTGTGRTFCGGFDLNTFTSFTNGEDIIKWFQYEEGVLLKLFRCPKPIVSAING
                     GATAAGMIYAMASDYNIVVNNPKVKIGMTEIKLGMSLSPCMGNVMRFGLDSDRNYRDI
                     IMKGELVDPMFCVERGIFDELAETDELVDKAKAKISSLYDTLLHPFTLLKDLQKRQMV
                     AVIEKELAEYDWNMMIDTFMDEKVNKLLKKVLASIS"
     gene            complement(2704..3138)
                     /locus_tag="P5549_07210"
     CDS             complement(2704..3138)
                     /locus_tag="P5549_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRW12897.1"
                     /translation="MFNKILVPVDGSEVSNKAIDAARLLIKEGSAREVTILHVLQPSE
                     VMPFNGLNMPVDYPQFYEELNKAAKRVLENAEQRMNLKEQTKIQLEYGPPAEIVCKAA
                     EQEKYDLIIIGNRGLNRLQRVLMGSVSSKVVTLAPCPVLVIK"
     gene            3459..4046
                     /locus_tag="P5549_07215"
     CDS             3459..4046
                     /locus_tag="P5549_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12898.1"
                     /translation="MNKLNRHVYVDEVIANSIDIDPQAMYYVWEKTNSLMSQSTRLRE
                     KDDRLSAFVDEIIMGIDRNLEKYISRHSQTHGAFYQNDLDRWIEAQPELKFVQDHLTE
                     WAVPHAQHDFVWFEKPFTGMEILVPEQSLPTMQDRQTFESELSNDFIWFEGSLDRWIE
                     AQPDLKQALDRWLDTTPADEANQKASQPIRKDKVA"
     gene            complement(4147..4929)
                     /locus_tag="P5549_07220"
     CDS             complement(4147..4929)
                     /locus_tag="P5549_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12899.1"
                     /translation="MAEYEYLSLQVEGPVATVTFSRPDRMNAMGIGPWKEIHLVQDEI
                     EANRDIRVVIIKADGPHFSVGINLNDLAGVSSDYVIDNINYLQRTYTRWQEMNAIVIA
                     AVHGLCYGAATEMLCACDIRVAAEDARFGMQEVRFGLSPDMGGSQRLPRLVGAGQAKR
                     IILACEEIDAKEAREIGLVEIVVPREELYERAGKLAKRIVNNPPWAVRFGKKAINASL
                     DSSLAGGLLLEQIQSTFCCGTADQKEAIQSFFEKRKPDFQDK"
     gene            5032..5850
                     /locus_tag="P5549_07225"
     CDS             5032..5850
                     /locus_tag="P5549_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRW12900.1"
                     /translation="MEQLPGDEKKEHLHEPQEWEWQLAEQEFKEHRDASRKRSHWLIV
                     PIVLLLVAAFTFLSLPDLQILMADRFEFMSDHARLRDDAIVTAAKPAVVAIEASVGNA
                     LSHSVRQGTGFNLSPQGRIITNLHIVEDASKIKITFGNGQTFIIDQYSVVAGHDLAMI
                     ELDSRDLPSLAASPQGKPEHGEQVTIIGNPLGLQKIAQRGRVGQYHMTQSNQTLVFDV
                     DVPANPGNSGSPVLNQQSQVIGVVFASTEIKKGEQIEIRTLAIPIQVLQYFPAT"
     gene            complement(5847..6329)
                     /locus_tag="P5549_07230"
     CDS             complement(5847..6329)
                     /locus_tag="P5549_07230"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRW12901.1"
                     /translation="MEYISYFVTDIGTLGITEKAGAITRLFLPGEPSFSNHLESMTPT
                     LEEARRQLLEYFQGHRKMFDLPLAMTGTKFMRSVWQSLLTIPYGQTRSYGDIARMIGN
                     KNACRAVGMANHSNPIAIIVPCHRVIGANGQLVGYGGGLKVKSYLLELEKHYASPGQG
                     "
     gene            complement(6376..8316)
                     /locus_tag="P5549_07235"
     CDS             complement(6376..8316)
                     /locus_tag="P5549_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HRW12902.1"
                     /translation="MYQQELIVNGELEQAWREFVQDGVINQNIVRPEIARSWFRCKKT
                     GNTADMQPLPDHLLRAKQENNRKMIEASRSILQDVAVMLAASMPCFAVILLDNEGDII
                     EMIDHNTGLLCLGHHCSELKSGTNAGGIALIDGAGVEVVGYEHLYPFAHKWHTLAVPL
                     RNSHRQVAGVFGVLNTEGPCPVLAMQTVSLGAQLIETRLQRDQIFVDVSGLMGERILE
                     PAILVNQNGLILDANQPFADLFMQEHDDLRGNNINACLRGEINFKTLLSPAIHLDHPV
                     SVSIQPFNQKLKKSSFVCSLSKYIVKADNENGLIIFTFSGINHNECQSINKSLTDRQE
                     DAFACLIGNNEAFLKVIKSARRAARISSTILIEGRSGTGKELMARAIHQESKRSGAFL
                     ALNCGSLPKELLYSELFGYEDGAFTGARRGGNAGKFELADGGTLFLDEIGEMPLDMQV
                     SLLRFLEDKTVTRIGGSKTKRVDVRIVAATNRNLTEEVQQGNFREDLFYRINVIHLTM
                     PALLTRKDDLDLLVKHLLHQLELKHGFDNIVISADVMKRFEAHDWPGNVRELQNVLES
                     SLIAADQGMITLECLPDYLQQNARPVRPAVQGNLREIEKYMIMETLQRNDHNISQAAR
                     ELGITRTTLYKKMEQMGLKRTV"
     gene            8709..9488
                     /locus_tag="P5549_07240"
     CDS             8709..9488
                     /locus_tag="P5549_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HRW12903.1"
                     /translation="MANIISCYKWVFDEEDIRINPADLSVDCSKAKSKISEYDRNAIA
                     VAVTTGEQRGDQVISLTFGNAGVKQSLKDVLSRGPAEAFWISDALAEKADGWVTANVL
                     AAAIEKIGDYSLIVCGEGAADTYAHQIGPRLGALLNIPVISCVAGIELDGNKITAIRK
                     LEDYRETVKATLPVVITVLPEIMEAPIPGLKAVMVAGKKPNTELKITDLGLTENELRP
                     CIKPIDMRGYANNRKNIVISDGNAAAKVTELIGHLKKEGVL"
     gene            9488..10405
                     /locus_tag="P5549_07245"
     CDS             9488..10405
                     /locus_tag="P5549_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRW12904.1"
                     /translation="MSGIFIYAEKPEASAELICLAKELGQSTGVIALNAADAEQIAQY
                     GANQVFWLKGTSQWPEDYAPAIVELLKAENAEMLLVGASVRGRDIAASVAARMHCGLV
                     SDVVSVKRNGNDIETTRMMYGGAVVQTALCQGFNVITVPPGKYPLANAGAFISPVLER
                     EVEAKPQVDLLENIQIEKQGVDLAQAKRVVCIGLGLEDQNELKMAEDLCESFEAALAC
                     TRPIAEEKGWLPADSYIGISGKSIRPELYVGIGVSGQVQHTVGIRDAKIIVAVNNNEK
                     APIFRIADYGIVGDLKEILPVLTQAIKKA"
     gene            10428..11717
                     /locus_tag="P5549_07250"
     CDS             10428..11717
                     /locus_tag="P5549_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW12905.1"
                     /translation="MSEDRFDAIVVGAGPAGIASAYILAREGKNVLVIERGNSAGDKN
                     VTGGRLYSYALDLVESGLWQEAALERKVVREKTMIMHGEQAITVDYLDPAVTSAGQRP
                     QSYTILRAVFDEWFAAQAEEKGAIIACGVKVDELLQENGRVIGVKAGEDEMLADIVIA
                     ADGVNSFMAQQAGLIHDITAESVAVGVKEVIELPAKTIEERFHLAPEEGAAMMILGCT
                     EGIHGGGFLYTNRDSISLGCVFMPEEAARHGRQVHDIMQDFKMHPAIYPMIADGQTIE
                     YSAHLVSEAGYRGIPKKIYKDGFLMVGDAAGFVINTGYSIRGIDLAIVSGVAAARAIL
                     AEPDAGKVGLRYMQELKELKMLPTMQAVDGYFDLLEIQRIYKHYPAMANDLAAFMYKA
                     DGNVASSLKKAIRPIMKRNGITMWQLLKDGIRGYKSI"
     gene            11714..12007
                     /locus_tag="P5549_07255"
     CDS             11714..12007
                     /locus_tag="P5549_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRW12906.1"
                     /translation="MSIEKMEAAELLSLNKFTVDEEEAHIVLDKNICRQCTEKACLLV
                     CPAVLYKLDKNGDISFDYAGCLECGTCRVMCKNKGIIKWQYPRSSFGVSYRNG"
     gene            12104..13261
                     /locus_tag="P5549_07260"
     CDS             12104..13261
                     /locus_tag="P5549_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW12907.1"
                     /translation="MWYLTEERELMRNIAREFVEKEVKPVALQIDKNCEFPMALFKRA
                     GELGFLGVTFSEEYGGLGGDQTTLALILEEIAKSCPVLTVAMGAHSLLAGGLLDMLGT
                     PEQKKKYLAPAATGEIILACGSTEAVGGSNQIEFTTRADLDGDEWVLNGGKVLITNIG
                     VADVYVILAVTQADVAPGAKLGLSTFIVEAGTPGFEVGKPEHKLGWHGSMTGSISFKN
                     CRIPKENLLGPLGSVLQAMFVSATAEFLSCGPVSLGIAEGAYEMALQYSMERVQCGKS
                     MYDRFQVSRHKLAKMWGEIESLRALIYSTYAMRDQGSLELARGRLLKVKGAEVSEYVC
                     REAIQLYGGVGTIIDTGVERFWRDAKVMAIGGASVEALMEQITMMIKNKMV"
     gene            13341..14141
                     /locus_tag="P5549_07265"
     CDS             13341..14141
                     /locus_tag="P5549_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW12908.1"
                     /translation="MYPYEKKYDHVIKLEKEGHIAIITFDCPENKNRLGEQTIDEYTA
                     ALENCHWDKEIRVIILRGTGDWCFGPGDLPIIQSKLAKSLPDGREVMYEIANMIKKMY
                     TISKPIIGVAESGCIGGGCNLLLSSDIVIASENAYFMQVFADYALSPDTGGLWALQRL
                     VGPMKAKVLSMTAEPLPAKAALEMGMVYKVSTDAYADAYALATKIAAKSPVGINHIKQ
                     ISNRLHDYTMDTYFQIEGDYLTLGALSHDFKESNIAAAQKRAPEYKGY"
BASE COUNT         4100 a         3255 c         3463 g         3772 t
ORIGIN      
        1 gtattatggg ctttgtcaac gcaccttccc tatttcaact ggtcattgcc cgctgcctgg
       61 aggccaaagt ggatgtagaa gcctacaacc gcaatcgtga acttatatat catgcgctgc
      121 gcgactacgg atacgaatgc atcaaaccgg aaggggcttt ctacctgttt gtcaaagccc
      181 ccaacgatga tgacgttgcc ttctgcaacc tggccaaaca aaaaaacatc ctggttgtcc
      241 cgggcagttc ctttggctgc gccggatatt ttaggattgc ttattgcgtg aatgaagaaa
      301 ccattagaaa atccctgcct ggttttgaaa cattgataaa ggaggtcaaa ggttaattaa
      361 ttgcgattgc gtgtcagttt tcctgagctt ttaccatagt ataatatgtg atacaatttc
      421 cttgacaggc gaaatttgga tattttcatc aagtcccaac caatatattc attctgcttg
      481 gcaagtcgat aaaaagtgag gaggttgtcg tattgaaaaa ctataaaaga gatctgttga
      541 tagctctggc gtcattgtta atcgttgttt ttggtgctgt cacggtctat ggggcaccga
      601 gcgtttctac cgtggtacaa gcctacacgg gtgtgaaaat cgtttataac ggaaccacgc
      661 tgacttccac cagtgaacct tacattatca acaacactac ctatgtacct attcggatgc
      721 tcatgaatca atttggcaag gatatttcct gggacggcga caccatgcag gtgattatca
      781 agaatagtgc cggagaaaac gccaaagaca aacaaatcga agacttgaaa aacgaagtca
      841 ctgctctaaa tgccaaaatc aaaggtttgg aaacggctct gggcaatgcc gatacaacct
      901 tgagaagcat agatgatgcg cttgacaaat atcagaaaac tgctggtacg gcagtaacca
      961 cgccggtcac accgggaatc agcactggta gcaccttgag tgatttccag agtaatctca
     1021 acaaatattt tgaggatgcc ggagatgagt attttgacga tgacggaatt gaatttagta
     1081 ttaatttaag cggcaatact gtcgacctgg tttacgatat cactctggat tttgatgacg
     1141 cagatgagta tgatcgcctc aaccagatta gtcagtccga tgtaaagact cttctgaaca
     1201 tggtgaaagc aaaaatcact tctgaggttt acggaaccaa ttacaaagat gccaaaatta
     1261 ctggaaaact gagcgatcag gataattcca gctactatgt taaatttaac ggcagttcct
     1321 acagctatag ttgggatgaa aatgatgtca ttgataatgt gatagaagcc ttggaggatg
     1381 agtatgaaaa cggcggcgat gtttattttg gagatgaaga tatcgctttt gaattcgaaa
     1441 tcaacgggga tgaagatgat cttgaatatg aaatccatat gaattttgac gatgcttatg
     1501 attatgataa tttaagggaa attaacaaaa cctatgtacg aaacttcttg aacaaagtta
     1561 aatcaaccat aagtgatgaa atagaagatt ctgattatga agacgctgac atcacgggct
     1621 atctttatga tgatgataat gatgattact atgttaaata caatggcagc tcttatactt
     1681 ttagctatta aataaagaaa aatattagta aatatttgcg ttaaggaata taatgatttt
     1741 aagtaataaa tatttattat attaatggag gtaatgaacg tgacatttga aaccagaatc
     1801 gaagacggga tccttatttt cacctttaac tatcccaaag taaatgcgat caacatggaa
     1861 accttgcagg gattggatgc agcgcttgac caactccagt ctgacgacgc actgaaagga
     1921 atggttctga caggaaccgg cagaacattc tgcggaggat ttgatctcaa tacgtttaca
     1981 tcatttacga acggagaaga tatcattaaa tggtttcaat acgaagaagg tgttttgctg
     2041 aaactgttta gatgccccaa acccatcgta tcagccatca acgggggagc gacggcagct
     2101 ggcatgatct atgccatggc ctccgactat aacatcgttg tcaacaatcc caaagtcaag
     2161 attggcatga ctgaaattaa attaggcatg agtctgtccc catgtatggg caatgtcatg
     2221 cgctttggcc tggacagcga cagaaattac cgtgacatca tcatgaaagg ggaactggta
     2281 gatccaatgt tctgtgttga gcgcggcatt tttgatgaac tggcggaaac ggatgaactg
     2341 gtggacaagg ctaaagccaa gatatcttct ctttatgata cgttgctgca tcccttcacc
     2401 ctattgaaag atctgcaaaa acggcagatg gtcgcggtca ttgaaaagga actggcggaa
     2461 tatgactgga acatgatgat tgatacgttt atggatgaaa aagtaaacaa attattgaag
     2521 aaagtattgg cttcgatttc ttaaacagaa ggggataatc ttccctgcca acggaaaatc
     2581 taataattgc catacaaaaa gcccgtccag gtacgggctt tttgtaatca ttcgcagttt
     2641 tccaggcttt gctcagcaac attatatcga tgaaatcact aagcaagaac agtatgctaa
     2701 tgtctattta atcaccaata ccgggcaggg ggccagggta accaccttgc tgctgacgct
     2761 gcccattaaa acccgctgca gtctgtttaa tcctcggttg ccaatgatga ttaaatcgta
     2821 tttttcctgt tccgcggctt tgcagacgat ttcagcggga ggtccatact ccagctgaat
     2881 tttggtttgc tctttcagat tcattctttg ctcggcattt tccaacaccc gtttggcagc
     2941 cttgtttaat tcctcataaa attgggggta atcgaccggc atgtttaacc cgttaaaagg
     3001 catcacttca ctgggctgca atacgtgcag gatggtgact tctctggctg aaccttcttt
     3061 gatcaacaaa cgggcagcat cgatggcttt gttggaaact tccgacccat caacggggac
     3121 gagtattttg ttgaacataa cctttaacct cccttactat taaattattg atgaagtact
     3181 caaattaatg ccaaaggtaa cttgacaaat caatgcaaga cgatataatt tagttaagga
     3241 taatattacg aatacgtaat atatcgttat gtaattatat catgttttac aattataatc
     3301 ttattataaa cgttgcaacg ttattttttt aatcgataat tgtgaaattt atcacgttat
     3361 aaagtgattt attacgctga tagatggaac aacatcaata aataagatgt gcagttgcct
     3421 tgcaaacacg aaataatctt caaggaggtg ttgttaagat gaataaattg aatcgtcatg
     3481 tatacgttga tgaagttatc gccaatagta tcgatatcga tccccaagcc atgtattatg
     3541 tatgggaaaa aacaaactcc cttatgagcc agtccaccag gctaagggaa aaagatgata
     3601 ggttgtctgc ctttgttgat gaaatcatca tggggattga ccggaatctg gagaagtaca
     3661 tatcccgtca cagtcagact cacggtgcat tttaccagaa tgatttggat cgttggattg
     3721 aagcccagcc tgaattaaaa tttgtccagg accatttaac ggaatgggct gttccccatg
     3781 cgcaacatga ttttgtctgg tttgaaaaac cttttaccgg aatggaaata cttgtgccgg
     3841 aacagtcact cccgacaatg caggataggc aaacgtttga atctgaactg tcaaacgatt
     3901 ttatttggtt tgaaggatct ttggacagat ggattgaagc gcagcctgat cttaagcaag
     3961 cgcttgatcg ctggctcgat acaactccgg cagatgaggc caaccaaaaa gcaagtcagc
     4021 caatacgcaa agacaaagtt gcctaggcca tacagtaatc acatataaga aaaaccgggg
     4081 aatgccgacc ccggtttttc tgtccaagag aagaagcgag ataaaacgct tgccgtaaat
     4141 ctgctattat ttgtcttgga aatcaggttt tctcttttca aaaaaggatt gaatggcttc
     4201 tttttggtca gcggtgccgc agcagaaagt ggattggatt tgttccagca gcagaccacc
     4261 tgccaaactg ctgtccagtg aggcattgat ggcttttttc ccaaagcgta cagcccaggg
     4321 gggattgttg acaattcttt tggccagttt tccagctcgt tcatataatt cctcgcgcgg
     4381 cacgacgatt tccaccaacc caatctcccg tgcctctttg gcgtcaattt cctcacaggc
     4441 caggataatg cgtttggcct gtccggcccc taccagtctg ggcagacgtt gggaaccacc
     4501 catgtcagga ctcaagccaa agcggacttc ctgcatgcca aacctggcat cctcggcggc
     4561 aacccggatg tcgcaagcac agagcatttc tgtggcagcg ccgtaacata acccatgcac
     4621 ggcagcgatg actatggcat tcatttcctg ccagcgagta taggttcgct gtaaataatt
     4681 gatattgtcg atgacgtaat cagaactgac gcctgccaaa tcattgaggt tgatacctac
     4741 ggaaaagtgc ggtccatctg ctttgataat gaccacccga atatcacgat tggcttcaat
     4801 ttcatcctgc accaaatgaa tttctttcca gggaccgatg cccatggcgt tcatccgatc
     4861 cggacgactg aatgtcacag tcgcaaccgg accctctact tgcagtgata agtattcata
     4921 ttcagccatt ttataccctc cgttacaaat atatttgctt ttatttatta taatggatag
     4981 tagataaaag ataaaattac tcacaaaaga gttttaggca ggtatttgga catggaacaa
     5041 ttaccaggcg atgaaaaaaa agaacaccta catgaaccgc aagagtggga atggcagttg
     5101 gctgagcagg agttcaagga acaccgggat gcttcccgaa aaagaagtca ttggctaatc
     5161 gtgcccattg tactgctgtt ggtggcagct tttacgtttt tgtctctgcc ggacttgcag
     5221 attctgatgg ctgatcgttt tgaatttatg agcgaccatg cccgcttgcg tgatgatgcc
     5281 atcgtgactg ctgccaaacc ggccgtggtt gcgattgaag ccagtgtcgg caacgcactt
     5341 tcccattcgg ttcgacaggg aaccggtttt aatctctcgc cccaaggccg gatcattacc
     5401 aacctgcaca ttgtggaaga tgccagcaag ataaaaatta cctttggcaa tggtcaaaca
     5461 tttattattg accaatatag cgttgtcgca ggccatgatc tggccatgat tgagctggac
     5521 agtcgcgatc tgccgtcgct ggccgccagt ccccagggaa aacccgagca cggcgaacag
     5581 gtgaccatta tcggcaaccc cctggggttg caaaagatcg cccaacgcgg gcgggtcgga
     5641 caataccaca tgacgcaatc caaccagaca ctggtatttg atgtagacgt tcccgccaat
     5701 ccaggcaaca gcggcagtcc agtcttaaac cagcaatctc aggtgatcgg agtcgttttt
     5761 gccagtaccg agataaaaaa aggggagcag atcgaaatac gcaccctggc aataccgatt
     5821 caggtgctgc aatactttcc tgccacttaa ccctgacctg gactggcgta gtgcttttcc
     5881 agttccagta aatatgattt aaccttcaat ccgcccccat aacctaccaa ttggccattg
     5941 gcaccaatta cccggtgaca ggggactatg atagcgatgg gattgctatg attcgccatt
     6001 cccactgccc gacaggcgtt tttgttgccg atcatacggg cgatatcgcc atatgatcgg
     6061 gtttgtccat aagggatggt caacaaactc tgccaaacgc tgcgcatgaa ttttgtaccg
     6121 gtcatggcca gcggcaaatc aaacatcttt cgatgtccct ggaaatattc cagcaattgc
     6181 cgacgagctt cctctaaagt tggagtcatg gactccagat ggtttgagaa actcggttct
     6241 cccggcagga aaagacgggt gatcgctccc gccttttcag tgattccaag ggtgcctata
     6301 tccgtgacaa aataactaat gtattccatg ctttttccta ctttacatta tgttcggtga
     6361 caggatggca ttaacttaaa cagttcgttt tagtcccatc tgttccattt ttttatacag
     6421 ggtcgtccgg gttatcccaa gttcccgggc tgcctggctg atattatggt cgttccgttg
     6481 cagcgtttcc atgatcatat atttttcaat ctcccgcaga ttgccctgta cagcaggtct
     6541 gacgggacgg gcattctgct gcaggtaatc cggcagacat tccagggtaa tcattccctg
     6601 atcggctgca atcaagctgc tttccaggac gttttgcagt tcacgtacat tccccggcca
     6661 atcatgggct tcgaaacgtt tcatcacgtc tgcactgatt acgatgttgt cgaagccgtg
     6721 ttttaactcc aactgatgca gcagatgttt taccagtaaa tcaagatcgt ctttgcgggt
     6781 cagcagtgcc ggcatggtta aatgaataac attgatgcga tagaacaaat cttcccggaa
     6841 attaccttgc tgaacttctt ctgtcaggtt gcgattggta gccgcaacga tgcgtacatc
     6901 gactcgtttc gttttactcc cgccaattcg ggttacggtt ttatcctcca ggaaacgcag
     6961 caaactgacc tgcatgtcca agggcatttc gcctatttca tccaaaaaca gcgttcctcc
     7021 gtctgccagt tcaaacttgc cggcgttgcc gcctcttctg gcaccggtaa aagcaccgtc
     7081 ctcatagccg aacaactcac tgtacaacaa ttctttgggc agggacccgc agttcaaagc
     7141 taaaaaggcc ccgctgcgtt tgctttcctg atgtatggca cgagccatca attccttgcc
     7201 cgttccgctg cgaccttcaa tgagaatggt ggatgagatt cgggctgccc gccgggcaga
     7261 tttaatgacc tttaaaaaag cttcattgtt gccaataaga caggcaaatg catcttcttg
     7321 cctgtctgtc agggatttgt tgatactttg gcattcattg tggttgatgc cgctgaaggt
     7381 aaaaatgatc aagccgtttt cattgtcagc tttgactatg tacttgctta aactgcaaac
     7441 aaaggaactc tttttcagtt tctggttaaa cggctgaatt gagacgctca ctgggtgatc
     7501 aagatgaata gcaggcgaga gcaatgtttt gaaatttatc tcaccgcgca ggcaggcgtt
     7561 gatgttgttg ccccgcaggt catcgtgttc ctgcataaac agatcggcaa aaggttggtt
     7621 tgcgtccaga atcaaaccat tttgattaac cagtatcgcc ggttccagaa ttctttcccc
     7681 catcaggcca gacacatcaa caaagatttg atcccgctgt aacctggtct cgattaattg
     7741 tgctcccaac gaaacagtct gcatggctaa aaccggacaa ggaccttcag tattcaaaac
     7801 gccaaacaca cctgccacct gtcgatggga attgcgaagc ggtacagcga gcgtatgcca
     7861 tttatgggca aaaggataaa ggtgttcgta gccgactact tccaccccgg caccgtcaat
     7921 cagggcaatt cctccggcat tagtgcctga tttgagctca ctgcaatgat gtcccagaca
     7981 gagcagaccg gtattatggt cgatcatttc aataatatcg ccttcgttat ccagtaaaat
     8041 aacggcaaaa cacggcatgg aagcagccag catgaccgca acatcttgca gaatagagcg
     8101 ggatgcctcg atcattttgc ggttattttc ctgtttggcc cgtaatagat ggtcaggcaa
     8161 tggctgcata tcagcagtat tgccggtttt tttacatcta aaccaggaac gggcgatttc
     8221 cggacggaca atattttgat tgataacccc atcctgcacg aactcacgcc aggcttgttc
     8281 aagttctccg tttacaatca gttcttgttg atacattcat ttcacctgcc gttactttat
     8341 gttaaatcga catattgcga catcgcattg ctattcaaat tatacacctt gcaggtaaat
     8401 tgtaaaagac acttgcggag aaggattgga caatgcttaa attaccttcg ttgtatttgg
     8461 tttgcctcgt aatcataaat gtacccttaa tagtgtttaa tttattaaca ctgttcattt
     8521 acttgacaat catgccgact ataatttttt cgcagaagca tgtaatcttg ggttttggct
     8581 gatgataata attggcacgg tttttgcttt tattattgtc aatgaccaca ggcataaaaa
     8641 acggtaaaca atcaatgatg ccgtgttcac tgaagggatg aaaacgttta acagatggag
     8701 gtagaattat ggccaatatt atcagttgtt acaaatgggt gtttgatgag gaagacatcc
     8761 gtattaaccc ggcagatctg agcgttgatt gcagcaaagc aaaaagtaaa atcagtgaat
     8821 atgatcgcaa tgccattgct gttgctgtaa ccacaggcga acaacgtggt gatcaagtca
     8881 tctccttgac ctttggtaat gccggagtca aacaatctct caaagacgtt ttgtcgcgtg
     8941 gtccggctga agcattctgg attagtgatg cactggcgga aaaggcggat ggatgggtga
     9001 cagccaatgt attggctgcc gccatagaga aaattggaga ttacagttta atcgtatgtg
     9061 gtgaaggtgc tgctgacacc tatgctcatc agattggccc ccgccttgga gcattgctta
     9121 acattccggt tatttcgtgt gttgccggaa ttgaattgga cggcaataaa attactgcaa
     9181 tccgtaaact ggaagactac cgggaaacag tgaaagccac cttgccggtt gtcataacgg
     9241 tattgccgga aatcatggaa gcaccaatcc ctggcttaaa agctgtcatg gttgccggta
     9301 aaaaacccaa taccgagtta aaaattactg atcttggtct taccgaaaac gaactccgtc
     9361 cctgcatcaa acccatcgat atgcgagggt acgccaataa tcgtaaaaac atagtgatca
     9421 gcgacggcaa tgctgccgcc aaagttactg aattaatcgg tcatcttaaa aaagaggggg
     9481 tgttataatg tccggaattt ttatttacgc tgaaaaacca gaagcaagcg cagaattaat
     9541 ctgcctggca aaagaactgg gacaatccac tggtgtaatt gcgttaaacg cggctgatgc
     9601 tgaacaaata gcccaatatg gtgcaaatca ggtattttgg ttaaaaggta ccagccaatg
     9661 gccggaagat tatgccccag ccatagtcga gttattaaaa gctgaaaacg ccgagatgct
     9721 tctggtggga gcatcggtgc gggggagaga tattgctgcc agcgttgctg cccgcatgca
     9781 ttgcggcctg gtcagtgatg tcgtatctgt aaaacgcaat ggcaatgata tcgaaaccac
     9841 ccgtatgatg tatggcggag cggtggtgca aactgctcta tgccaagggt ttaatgtaat
     9901 cacggttccg ccggggaaat acccactggc caacgcaggt gcattcataa gtcctgtact
     9961 ggagcgcgaa gtggaagcaa agccgcaggt tgacttgctg gaaaacatac aaattgaaaa
    10021 acaaggcgtt gaccttgctc aagccaaacg agtcgtttgt atcggtttgg ggctggaaga
    10081 tcaaaacgaa ctgaaaatgg ccgaagactt gtgcgaatcc ttcgaggctg cattagcttg
    10141 tacccgcccc atagctgaag aaaaaggctg gctgccggcg gacagctata ttggtatttc
    10201 cggaaaatct atccgcccgg agctctatgt tggtatcggc gtatccggcc aagtgcagca
    10261 tacggtcggt atccgggatg caaaaattat cgtagccgtc aacaacaacg aaaaagcacc
    10321 tatttttaga attgcagact atggaatcgt aggcgatctc aaggagatct tgcccgttct
    10381 cacccaggca attaaaaaag cgtaaaagaa ttggggtgca aacgaaaatg agtgaagaca
    10441 gatttgatgc cattgtggtc ggggccggtc ctgctgggat tgccagtgcc tatattctgg
    10501 cccgggaagg aaaaaatgtg ctggtgatcg aacgaggaaa cagcgccgga gacaaaaacg
    10561 taaccggcgg ccggctgtat tcctatgccc tggatttggt tgaatcaggc ctgtggcaag
    10621 aagccgcgct ggaaagaaaa gtggtccgcg aaaaaaccat gattatgcat ggcgaacaag
    10681 cgatcaccgt tgattatttg gatcctgcag tgacctctgc aggccaacgg cctcaatcct
    10741 atacaattct gcgggctgtt tttgatgaat ggttcgccgc ccaagcggag gaaaaaggcg
    10801 ccataattgc ctgcggcgtt aaagtggatg agctcctgca agaaaacggc cgagtcatcg
    10861 gagtaaaagc cggagaagat gaaatgctgg cggatatcgt catcgccgcc gacggcgtca
    10921 attccttcat ggcccagcag gcgggtctga ttcatgatat caccgccgaa agcgtggccg
    10981 tgggcgtaaa agaagttatt gaactaccgg ccaaaaccat tgaggaacga ttccatctgg
    11041 caccggagga aggggccgcc atgatgatcc tgggctgtac cgaaggcata cacggcggag
    11101 gttttctcta caccaaccgt gacagcatct ccctgggatg tgtctttatg ccggaagaag
    11161 ctgcccggca tggacgtcag gtgcacgaca tcatgcagga ctttaaaatg caccccgcca
    11221 tatatcccat gattgccgac ggacaaacca tcgaatacag cgcccatttg gtcagcgaag
    11281 ccgggtatcg gggaattccc aaaaagattt acaaggatgg cttcttaatg gtaggggatg
    11341 cggccgggtt cgtcatcaat accgggtaca gcatcagagg gattgacctg gctatcgtca
    11401 gcggcgtggc cgctgcccgg gcaattctag ccgagcctga tgctggcaag gtcggccttc
    11461 gttatatgca ggaattgaaa gaacttaaaa tgttgccgac catgcaagcg gtcgatggat
    11521 attttgatct gctggaaatc cagcgtattt ataaacacta tcccgccatg gccaacgacc
    11581 tggctgcttt catgtacaag gcagacggaa acgtagcctc aagcttgaaa aaagccatca
    11641 gacccattat gaaacgcaac ggcattacca tgtggcaatt gttgaaggat ggcatcaggg
    11701 ggtataaatc gatatgagca tagaaaagat ggaagcagct gaactgctca gtttgaataa
    11761 attcactgtc gacgaagaag aagcgcatat cgtcctggac aaaaatattt gccggcaatg
    11821 cactgagaaa gcctgtctgc tggtttgtcc tgccgttctc tataaactcg acaaaaatgg
    11881 cgacatcagt tttgattatg cgggttgttt ggaatgcggg acatgccggg tcatgtgcaa
    11941 aaacaaaggg attattaaat ggcaataccc ccgcagttca tttggggtta gttatcgcaa
    12001 tggatagcgt tgtatacata cttggaaagg agggtgaatt ttttcaaatt acagttcccg
    12061 atggcaagtg aataaaaagc aattaacagg aggttgattt taaatgtggt atttgacaga
    12121 agaaagagaa ttgatgcgta atatcgcccg tgagtttgta gaaaaagaag taaaaccggt
    12181 cgccctgcag atcgacaaga attgcgagtt ccccatggcc ctttttaaaa gagcaggtga
    12241 actcggcttt ctcggcgtta ccttttccga agaatatgga ggattaggcg gagatcaaac
    12301 caccctggcc ttgattttag aagaaatcgc caagagctgt ccggttctga cggttgccat
    12361 gggcgctcat agcctgctgg ccggcggact gctcgatatg ctgggcactc ctgaacaaaa
    12421 gaagaaatac ctggccccgg cagctacagg agaaattatc ctggcttgcg gttcaacgga
    12481 agcagtaggc ggttccaatc aaatcgaatt taccactcgc gctgacctgg atggagatga
    12541 atgggtactg aatggcggca aggtactgat caccaatatt ggtgtggcag atgtttatgt
    12601 tattctggcc gttacccagg cagacgttgc tcccggcgcc aaactgggtt tgagcacatt
    12661 tatcgtggaa gccggaaccc ccggatttga agtcggcaaa cccgagcaca agcttggttg
    12721 gcacggttcc atgactggca gcatttcctt taaaaattgc cgaatcccca aagaaaacct
    12781 tttgggaccg ttgggcagtg ttctgcaagc catgtttgtc agcgccactg ccgaattctt
    12841 aagctgtgga ccagtcagcc ttggcattgc cgaaggtgct tatgaaatgg ccctgcaata
    12901 cagcatggaa agagtgcaat gcggaaaaag catgtatgat cgcttccagg tcagccgcca
    12961 caagctcgcc aaaatgtggg gagaaatcga atctttgcgg gccttgattt acagtaccta
    13021 tgcaatgcgc gaccagggca gcctggaact ggcccgaggt cgcctgctca aagtcaaggg
    13081 tgccgaagtg tctgaatacg tttgccgcga agccattcag ctgtatggcg gggtcggcac
    13141 gattattgac accggagtgg aacggttctg gcgtgatgcc aaggtcatgg caatcggtgg
    13201 tgcttccgta gaagctctga tggaacagat cacgatgatg ataaaaaata aaatggttta
    13261 accatccggt ttggaaaatc actacaattg ctttaattgc ttattacaca acaacaaata
    13321 aatgtgagga agtgacatta atgtatccct atgaaaagaa atatgatcat gtcattaaac
    13381 tggaaaaaga aggacacatc gccatcatca cgtttgattg cccggagaac aaaaaccgtt
    13441 tgggtgagca gaccattgat gagtatactg ctgccctgga aaactgccat tgggacaagg
    13501 aaatccgggt cattattctg cgtggtaccg gcgattggtg ttttggcccc ggcgacttgc
    13561 caatcattca atccaaattg gccaagagct tgcctgacgg acgggaagtc atgtatgaaa
    13621 tcgccaatat gatcaagaaa atgtatacga tctccaaacc gattatcggt gtggcggaaa
    13681 gtggctgtat cggtggcgga tgcaacctgt tgctgtccag cgacatcgtc atcgcttcag
    13741 aaaacgctta ttttatgcaa gtttttgccg actatgccct cagccctgac accggaggac
    13801 tctgggctct gcaacgcctg gtagggccca tgaaggccaa agtcctgtcc atgaccgcag
    13861 aacctcttcc tgccaaagct gctctggaaa tgggcatggt ctataaagtg agtactgacg
    13921 cttatgctga tgcgtatgcc cttgcaacca aaattgctgc caaatcgccg gtaggcatca
    13981 accatattaa acaaatcagc aaccgcctgc acgactacac gatggacacc tatttccaaa
    14041 tcgaaggcga ttatctgacc ctgggcgccc tcagtcatga ttttaaagaa agcaatattg
    14101 ctgctgccca gaaacgtgct cccgaataca aaggatacta gacgaaagaa taccacccgg
    14161 ccaaaaaggc cggaagatgt atttatcttt acccgggctc cgggagaaag caaaaggatg
    14221 atttataaat catccagata tcctgccagg cattcatctt attttgcgac caaattcgaa
    14281 gtgaccaatg caatcgatcc gtatctggcc aacaactttg ccagaaagct acagattata
    14341 tggcaagccc cagccccgcc atcggtggtg ccatcgtcgg actttgaata tttattgata
    14401 cacacccaat aaaatcagaa agccggtctt ttttagaccg gcttttattt aaagggctgc
    14461 cgaatgaatg gatttgataa tataccagat tcaaatttat tactttcgct ccaccggaag
    14521 tgctgatttt actaatggta cgcccgagag gaatcgaacc tccgcacccg gctccggagt
    14581 acgggtaaac
//
LOCUS       DAOTHZ010000054        14387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5054_length_14387_cov_6.687273, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000054 DAOTHZ010000000
VERSION     DAOTHZ010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 14387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14387
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_14387_cov_6.687273"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..282
                     /locus_tag="P5549_07270"
     CDS             91..282
                     /locus_tag="P5549_07270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRW12909.1"
                     /translation="MITPSTKELMDLSQSKYAVVVAVAKQARKLSENKKNDENYRLSS
                     MVTEALEEVMSEKVTIVYK"
     gene            308..1510
                     /gene="coaBC"
                     /locus_tag="P5549_07275"
     CDS             308..1510
                     /gene="coaBC"
                     /locus_tag="P5549_07275"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRW12910.1"
                     /translation="MVKTVGIGITGGIASYKIADLVSKLKTNGFDVIVMMTEGATRFI
                     SPLTFKTLSGREVATDLWSESLEYKVQHIGIAEKLDLLVIAPATANFIAKMAHGIADD
                     LLSTVVVANTSPVLIVPAMNSNMFKNPIVQDNLKKLQSYGYYVQEPDSGLLACGVEGK
                     GRLPAIEDIYEQIAGLLEPRTDMAGKRVLVNAGSTCEDIDPVRFIGNRGTGRMGYAIA
                     REACERGAEVILVSGKTNLEVPANVKCINVWSSQEMCDVMLQYQPVCDVIIGAAAVGD
                     FRIAQVAAQKIKKTDDGPPSVMLELVGTTDILKEIGKRKQENQIMVGFAAETENLLEN
                     ARKKLTSKNLDFIVANDVTVEGAGFATETNVVTLISRNGEVESLPKMLKEEVAAAIID
                     RVVALTQA"
     gene            1535..2725
                     /gene="metK"
                     /locus_tag="P5549_07280"
     CDS             1535..2725
                     /gene="metK"
                     /locus_tag="P5549_07280"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRW12911.1"
                     /translation="MSKKLFTSESVTEGHPDKMADQISDAILDSILAQDPFARVAAET
                     IVTTGLVLVSGEITTECYIDIPKLVRNTIKDIGYTRAKYGFDSETCAVLTSLDEQSGD
                     IAMGVNQALEAKRGEMVDADVSTIGAGDQGMMFGYATNETDEYMPMPILLASKMAKRL
                     TDVRKEGIIPYLRPDGKTQVTVEYIDGKPARVDTVVVSTQHHPEVNLATIESDIIEQV
                     IRQVVPAAMLDNETKYYINPTGRFVIGGPQGDCGLTGRKIIVDTYGGMARHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNMVAAGVADRLEIQLAYAIGVAQPVSISVDTFGTNKIAN
                     EKIVSLIKEYFDLRPAAIIRDLDLRRPIFQKTAAYGHFGRNESEFTWERTDKAGAIRN
                     AAGL"
     gene            2829..5219
                     /gene="priA"
                     /locus_tag="P5549_07285"
     CDS             2829..5219
                     /gene="priA"
                     /locus_tag="P5549_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRW12912.1"
                     /translation="MQAVSVIINLHTQQLNNRFLYLVPEELQAEALFGKRVLVDFRGR
                     KTEAFVVDQEDNSLAAEFKPLIRVLDQEAVFDQELYDLAEWMAAYYAASLAQILSMMI
                     PRLLHQQKLRRVISLVSQAEYEQSHTVKNDSGKALMELLWNQGEASVNETLKIISKEQ
                     LEELIENGLVYLTGVYGATRTSKSDMVYILDDEQYTSQIDAIRKKAPRQAEALDLLVS
                     HPQMSCHEFDMMVSRSAGNALRQKNLIRTIRRQPVIQTPAFELNSEQKHALQAIRDGL
                     AAAKHREFLLFGITGSGKTEVYIEACQQAIQQGKRVIVLVPEIALTRQLVDIFSHRIA
                     DMAVLHSAMSAGERYGEWKRIKRGEVQLVLGPRSAIFAPLPDLGLIIMDEEQENSYKQ
                     EETPRYHARDIARQRARITKAVLLLGTATPALETYYRTTAGDAQLLTLSKRTGAAAIP
                     MIVIEDMKKTFKSGYPGMLSRYLQERLEHVLHRGEQSILFINRRGHSPMTICRECGNI
                     ASCPNCAVAMTYHHDIRQNVCHYCNVQRPQHSVCPTCGSQHLQMMGTGTQKVEEEIRQ
                     LYPTARIARLDLDSSRAKGAQKAILADMKAGRIDILIGTQMVAKGFDFPRVSLVGVVD
                     ADSLLNLPDFRARERCFQLLVQVAGRAGRANIPGEVVIQTYNPQEPLFEKVVLQDYKR
                     FYEDEIEIRHRLDYPPFTEILRIVVSSEEDASGQAYINILARQIEEMIDAKEDSIMIL
                     GPAPCPISKIKSRYRYQLMIKCNSVLLLGSIAAKINQITHPANLRVEMDINPVVTI"
     gene            5233..5688
                     /gene="def"
                     /locus_tag="P5549_07290"
     CDS             5233..5688
                     /gene="def"
                     /locus_tag="P5549_07290"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRW12913.1"
                     /translation="MSVYKIVTVPDPILRQKAQKVEKVNAGVLRLLDNMRDTMYAADG
                     IGLAAPQIGVSKRVIVIDPGDQLIEMINPEIITREGEQTAREGCLSVPDLVGWCKRAQ
                     LITVRGMNRDGEEIEMEASDLLARAFQHEIDHLDGILFPDKAIQTRKEK"
     gene            5713..6645
                     /gene="fmt"
                     /locus_tag="P5549_07295"
     CDS             5713..6645
                     /gene="fmt"
                     /locus_tag="P5549_07295"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRW12914.1"
                     /translation="MKIVFMGTSAFSVPSLKRLMDTEHEITGVVTQPDRPRGRGQKLI
                     PSPVKELALNHHLAIYQPEKIRAEEAIDHVRSLDPDLIVVVSYGQIIPPEILDFPKLG
                     CINVHASLLPRYRGAAPIQRAIMAGETVSGVTTMAMDRGLDTGDIILQLPVKIGADMD
                     YGIYAEILANAGADLLIDTLAIIRSGSYPRRKQDSAKATYAPMIQREEERIQWQQPAL
                     VINNQIRAFSPQPAAYTSFNNQRFKVFSSRLLNENTAAAPGEIAHINASGIVVQCSEG
                     MLEIKEVQLAGKKRMPSNEFVRGFKLVPGDVLGE"
     gene            6690..7439
                     /locus_tag="P5549_07300"
     CDS             6690..7439
                     /locus_tag="P5549_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HRW12915.1"
                     /translation="MESKKRIYIGLLIISLVTMALFMPLLVFLLQQNTPVSKIVLTVI
                     AILIGGIFFLLGLGIMAIIIMIIRSKTNPSLDSIAKLANELLFPITLVIGKVLAIPPE
                     KIRSSYIAVNNYLVKSKHIILKGENVLILLPHCLQNDECPHKITMDINNCKQCGKCCI
                     SDLIKLSHKYSAIIKVATGGTLARKMIKECRPSGVIAVACERDLSSGIQDSGSLPVYG
                     VLNRRPNGPCHNTSVDVAKVEQALIMICRGG"
     gene            7443..8114
                     /locus_tag="P5549_07305"
     CDS             7443..8114
                     /locus_tag="P5549_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRW12916.1"
                     /translation="MGYLILILPALILSIYAQFKVKSTFSKYSAVRSLKGITGREAAY
                     GLLRNNGINDVAIEAIRGSLTDHYDPSAHVLRLSETVYGNDSIAAIGVAAHEVGHAIQ
                     HQQGYKPMEIRQKIVPVTNFASTASFPILLIGMVIASTDLIMIGILLFSAVVLFHLVT
                     LPVEFNASNRAVVQLHQYGLISTEEVPLVKKVLGAAALTYLAAALSAIANLLYYLAIF
                     AGSDD"
     gene            8131..9504
                     /gene="rsmB"
                     /locus_tag="P5549_07310"
     CDS             8131..9504
                     /gene="rsmB"
                     /locus_tag="P5549_07310"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRW12917.1"
                     /translation="MRTQNDALLTDPREMAVTLVYQIMEKGAYANLSLDHALHGSKML
                     QKDKNLVTELVNGSIRMIKHLDWVLDLFLRKSIDEQNPWVRSALRVATYQIMFMERIP
                     DYAAVDSAVAFVRRSVNKTLAGVCNAVLRNLIRNKAEISYPPVNSPTYLAVLHSQPEW
                     LVERWTACYGVQLTTAMLAYMNQKPLLTLRVNSLRTEAAALAMELSREGTQVRPGRLP
                     GSLIIDSLEKSIEQLHTYQAGGFYLQSEAAMLAADILHPVAGDQVLDLCSGVGGKATH
                     FAEKMNNQGLVKAVELHAHKLTLLQKNCQRLGVTIVTGEHADLLAMEEAPVWPKVFLD
                     VPCSGLGVLNRRADARWRKDPGEIAALTKLQAAMLAKAGKMTAPGGILVYSTCTINTE
                     ENEEIVVKYLQDHPQFQLQSMAREMDHFQLDQPDREMAEKGWLTIIPGKYGCDGMFYA
                     RLRRSND"
     gene            9505..10584
                     /gene="rlmN"
                     /locus_tag="P5549_07315"
     CDS             9505..10584
                     /gene="rlmN"
                     /locus_tag="P5549_07315"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRW12918.1"
                     /translation="MNDQNKTELLGMNQSELEEFVTGIHEPKFRARQLYKWIYQKQTA
                     SFYEMSDIPRDLRFRLDEIAQISIPRFIKQRVSHDGTRKFLLEMNDKKKVETVIIPQN
                     AQKNSQYTVCISSQVGCPVGCVFCATGAGGFQRQLLAYEIVGQVLSARRELAKRLKRT
                     ENDLITNVVYMGMGEPLLNYDEMIKSLYLLNEHKGMNIGQRHITISTAGEVNGIRKLI
                     RENLQVTLAVSLHACNNELRNTLVPMNKKYPLETLLEAVKEYAQTSNRRVTFEYVMLD
                     EINIHKQDAEAMIRLLKPILANVNLIPYNEVDGLNFKRPPQHRIRHFYQWLTAGGLNV
                     TVREERGTDIEAACGQLAVKKDRQV"
     gene            10602..11309
                     /locus_tag="P5549_07320"
     CDS             10602..11309
                     /locus_tag="P5549_07320"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRW12919.1"
                     /translation="MKAAGLTDIGLVRKRNEDNYWLDTDYQLFVVCDGMGGHLGGDIA
                     SRLAIETITRELIKEDLDEFPAALEKAVQSANRIIWETGQATSDLNEMGTTLTAAVIR
                     GNSIWIAHVGDSSLYIIHDAQITKPTSDHTLAEQMRKDGLIAKDDERYQSFHHVLVRA
                     LGVENQVDIDIHQMEVTPGDWILLCTDGLSNLVEQTEIKALLEHENEPQTACQKLLEL
                     ALARGGYDNITIILIQM"
     gene            11327..13171
                     /locus_tag="P5549_07325"
     CDS             11327..13171
                     /locus_tag="P5549_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRW12920.1"
                     /translation="MDQIILGGRYELLEIIGEGGMAKVYKAHCRILDRIVAIKVLKEE
                     YSCDQGFVEKFKTEALSAARINHPNIVNIYDVGQEGDIYYIVMELVEGKTLKEIISTE
                     APLMFERAVDIAIMICDGVHHAHEKGIIHRDIKPHNILITEHGMVKVADFGIARAITN
                     ATITYGNQNIVGSVHYISPEQAKGEAVNRTTDIYSIGCVLYEMLTGRPPFSADSAITV
                     AMKHIHDEITPPRRFNPDIPAALESIIMLAMQKNPKQRFASAQEMRNALLSIHMYRPE
                     EHIQKRKKEKIITGTPVMFEGDESLKKKKKLSPLGIAIIVIAVLGLLSGALLSGGGLF
                     GKEVVVPNVEGMDYKLADEELSKVGLVMSVVGKEYSNEFEKDVVISQDPGKERKVKEG
                     RKVEVVISQGSELITVPNITGIDIQDAVVKLGNAGLNMGDIEKEYDEEKPEGMIISQD
                     PARGSKEEAGTSVDVVVSQGKQPEKIAVPDLKGLTLDKARTALDDKKLVLGEVKREES
                     TVYMADHIVKQDTDPGVMVEEGTRINVVISTGPGPQVKTKVLQFKLPREEETMNVRIE
                     VSDLHGERQSYNQMHHGDDIINTSINYYGAGTAEVFINDKSVKIYKLN"
     gene            13187..14068
                     /gene="rsgA"
                     /locus_tag="P5549_07330"
     CDS             13187..14068
                     /gene="rsgA"
                     /locus_tag="P5549_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRW12921.1"
                     /translation="MTITQEQGLVLKSYRGFYYVQDNHTRIIECRPRGKLKTRVLSGD
                     RVMFTPLEDDKGVLESILPRNNEMVRPRVANVSMVLIVMAYDRPLPSLTLLDRLLLLV
                     QYSQLQPCIVLNKCDLPVHQTVTEILDYYPRAGYPVHSVSARCNQGMEDLQQAIHGEI
                     AVLAGPSGVGKSSLLNHLAGLDLVPTQEVSQKIGRGRHTTRHVELYPLDNQSWVVDTP
                     GFSVLDMPRMRREELPGWFPEFNRFAETCKFANCLHFKEKECGVKNAVEQGEILTSRY
                     NNYLSMLEEVMENERCY"
     gene            14068..>14387
                     /locus_tag="P5549_07335"
     CDS             14068..>14387
                     /locus_tag="P5549_07335"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRW12922.1"
                     /translation="MVLVAPSILSANFAQLGEDCQRLEQAGADWLHVDVMDGHFVPNL
                     TIGPPVVRDIRRISHLLFDVHLMIEKPESLIPAFIDAGADLITVHAEACTHLHRIIHM
                     IKNAG"
BASE COUNT         4000 a         3222 c         3742 g         3423 t
ORIGIN      
        1 cagtctccag gaagccgtgg aaaaagtgta tgccatcatt gtggctgagc gctgccggaa
       61 caggaataac gataaagggg ggttggaaag ttgattacac catccaccaa agaattgatg
      121 gatctcagtc aaagcaaata tgcagtggtc gtggccgttg ccaaacaagc gagaaaactg
      181 tccgaaaaca agaaaaacga tgagaattat cgcctctcca gtatggtgac ggaagctttg
      241 gaagaagtaa tgtctgaaaa ggttacaatc gtctacaagt aataatgtcc gacaaggagg
      301 cccggatatg gtgaaaactg ttggcattgg cattaccggc ggaattgcct cttataaaat
      361 tgcagacctg gtcagtaaac taaaaaccaa tgggtttgat gtcattgtca tgatgactga
      421 aggcgcaaca cggtttatca gtccactgac ctttaaaact ttatcaggcc gggaagtcgc
      481 tactgactta tggtcggaat cccttgaata taaagtccaa catataggca ttgccgaaaa
      541 acttgatttg ctggtcatcg ccccggcaac cgccaatttt atcgccaaaa tggcacatgg
      601 aatcgcagat gatctgcttt ctacggttgt cgtggccaat acctctccgg tattgattgt
      661 gcccgccatg aattccaaca tgtttaaaaa ccccatcgta caggataatc tcaaaaaact
      721 gcagtcatac gggtattacg tgcaagaacc tgacagcggt ctgttggcct gcggggttga
      781 aggaaaagga cggctgccgg cgatagaaga tatctatgaa cagattgcag gcttgctcga
      841 accccggaca gatatggctg gcaaacgggt gctggtcaat gccggctcaa cctgtgagga
      901 tatcgatccg gtccgtttca ttggcaatcg gggaaccggc cggatgggct atgccattgc
      961 cagggaagct tgtgaacgcg gggcggaagt tattttggtc agcggcaaaa ccaatctgga
     1021 agttcccgcc aatgtcaaat gcataaatgt ctggagttcc caggaaatgt gcgacgtcat
     1081 gctgcaatat caaccggttt gcgatgttat tattggcgct gctgcagtgg gtgactttcg
     1141 catcgcccaa gtggctgcac aaaagataaa aaagaccgat gacggtcccc cgtcggtgat
     1201 gcttgaatta gttggaacga ctgatatact aaaagagata ggcaagcgca aacaggaaaa
     1261 ccagattatg gttggttttg ccgccgaaac ggaaaacctc ctggaaaatg cccgtaaaaa
     1321 acttacatcc aaaaaccttg atttcatcgt agctaatgat gtcacggtgg aaggggccgg
     1381 atttgctacc gaaaccaatg tagtaacgct gatttcacgc aacggagaag tggaatcctt
     1441 gccgaaaatg ctgaaagaag aagttgctgc tgcaattatt gaccgtgtgg ttgcgttgac
     1501 gcaagcataa aatggcagga aagaggagaa gaaattgagc aaaaaactgt ttacatctga
     1561 atcagtaacg gaaggacatc ctgataaaat ggcggatcag atttcagatg ccattcttga
     1621 ttccattctg gcccaggatc cctttgcccg ggttgctgcg gaaaccattg tcacgactgg
     1681 cttggtattg gtttcgggcg aaatcacgac ggaatgctat atcgatattc ccaagctggt
     1741 caggaatacg atcaaagata ttggttatac ccgggcaaaa tacggttttg acagtgaaac
     1801 ctgtgcggtt ctgacttcct tggatgaaca gtccggtgat attgccatgg gcgttaatca
     1861 ggctctggag gccaaacgcg gtgaaatggt cgatgcggat gtatcaacca ttggcgctgg
     1921 agatcagggc atgatgtttg gctatgccac caacgaaacg gatgagtaca tgcccatgcc
     1981 gattctgttg gccagcaaga tggccaaacg tttgaccgat gtcagaaaag aaggcatcat
     2041 cccttacctg cgcccggatg gcaagaccca ggtaaccgtg gagtatatag atggaaagcc
     2101 ggcccgtgtg gacactgttg tcgtttcaac ccagcatcat ccagaggtca atttggcaac
     2161 gattgaaagt gacattattg aacaggtgat caggcaggtg gttcctgctg caatgctgga
     2221 taacgaaacc aagtattata tcaatcctac cggtcgtttt gtcatcggtg ggccgcaggg
     2281 ggactgcgga ctgaccggca gaaaaattat agtagacacc tacggcggga tggcccgtca
     2341 cggcggtgga gctttttccg gcaaggatcc gaccaaggtt gatcgttcgg ctgcctatgc
     2401 agcccgatat gttgccaaaa acatggtagc agcaggggtg gccgaccggc ttgaaatcca
     2461 attggcttat gccattgggg tggctcaacc ggtatcgatt tccgtggata catttggcac
     2521 caacaagatc gccaatgaaa aaatcgtatc cctgatcaaa gagtattttg atctgcgccc
     2581 ggcagccatt atcagagacc ttgacttgcg tcggcccatt ttccaaaaaa cagccgccta
     2641 cggtcatttt ggccgtaacg agagtgaatt tacctgggaa cgaacggata aagccggcgc
     2701 catcagaaac gcagccggtt tataatcccc cagtcaggtg ctgataaaaa ggcgggcaat
     2761 ccccgccttt tatttttcgg taaaacttca tgattgtcgg tattttctta tttaacaaag
     2821 gagcagacat gcaagcggtc agtgtgatca tcaatctaca tacccaacaa ttaaacaacc
     2881 gctttctcta tttggtaccg gaagagctgc aggcagaagc gctttttggc aaacgggtgc
     2941 tggttgactt tcgaggccga aagaccgaag cgtttgtggt ggatcaagaa gataattctt
     3001 tagcagcaga gtttaagccc ctgatccggg tgctggacca ggaagctgtc tttgatcagg
     3061 aattatatga tctggccgaa tggatggctg catactatgc tgcttcgctg gcgcagattc
     3121 tatccatgat gattccgcgt ttactgcatc agcaaaaatt acgccgtgta atcagcttgg
     3181 tcagccaggc agaatacgaa caaagccata cggtcaaaaa cgattccggc aaagcgttga
     3241 tggaattgtt atggaatcag ggagaagcaa gtgtaaatga aaccctcaag ataatctcca
     3301 aagagcaatt ggaagaatta atagaaaacg gtctggtgta tctgaccgga gtatatggtg
     3361 cgacgcggac cagtaaaagc gacatggtat atattctcga tgacgagcaa tataccagcc
     3421 aaatcgatgc cattcgcaaa aaagctccgc ggcaggctga ggcgctcgat ttgctggttt
     3481 cacatccgca gatgagttgc catgagtttg atatgatggt gagccgctca gcaggcaacg
     3541 ctttgcgcca aaaaaacctg atacggacaa tccggcgcca gccagtcatc caaacacctg
     3601 cttttgagct taattctgaa cagaagcatg ccctgcaagc gattcgagac ggcttggctg
     3661 ctgcaaaaca cagggaattt cttttattcg gaatcaccgg cagcgggaaa acagaagttt
     3721 atatcgaggc gtgtcaacaa gccatccaac aaggcaagcg agtcattgta ctggtgccgg
     3781 agattgctct gacccgccag ctggttgata tattctcgca tcgcattgca gatatggccg
     3841 tcttgcacag cgccatgtca gccggggaac gatacgggga gtggaagcga atcaaacggg
     3901 gagaagtcca actggtgctg ggtccgcgtt ccgccatttt tgctccattg ccggaccttg
     3961 gtctgatcat aatggacgag gagcaggaaa acagttataa acaggaagaa actccccgtt
     4021 atcatgcccg tgacattgcc cgtcagcgcg cccggattac gaaggcggtt ttattgctgg
     4081 gcactgctac ccctgctttg gaaacctatt atcgaaccac tgccggggac gcccaattgc
     4141 tgactttatc caagagaacc ggcgcagcag ccatcccgat gattgtcatc gaagacatga
     4201 aaaaaacttt caaaagcggt tacccgggga tgctttcccg gtatctgcag gaacgacttg
     4261 aacatgtctt gcaccgggga gaacaaagca ttctttttat caaccggcgc ggacattccc
     4321 ccatgacgat ttgccgggaa tgtggtaata tagcctcatg ccccaattgt gcagtggcca
     4381 tgacctatca tcatgatatc cggcaaaacg tttgccatta ctgtaatgta cagcgtccac
     4441 agcattccgt ctgcccgacg tgcggcagtc aacatctgca gatgatgggg acgggaaccc
     4501 agaaggtaga agaggaaata cgtcaattgt atccgaccgc caggattgca cgactggatc
     4561 tggacagcag tcgggccaaa ggagcgcaga aggctattct ggcagacatg aaagcaggac
     4621 gtattgatat tctgattggc acccaaatgg tcgccaaagg ctttgatttc ccgcgggttt
     4681 ccctggtagg tgtggttgat gccgacagct tgttgaatct acctgatttt agagcccgtg
     4741 aacgttgttt ccaactgctg gtgcaggtgg cagggcgggc gggaagagca aacatcccgg
     4801 gcgaagtagt gatccagacc tacaaccctc aggaaccatt gtttgaaaaa gttgttctcc
     4861 aggattataa gcgtttttat gaagatgaaa tagaaattcg ccatcgtttg gattatccgc
     4921 cttttactga aattctgcgc atcgtggtta gcagtgaaga ggatgcgtcg gggcaagcgt
     4981 acatcaatat tttggcaaga cagatagaag agatgattga cgccaaagaa gatagtataa
     5041 tgatcttggg gccagccccc tgcccgatca gcaagataaa gagccgctat cgttatcaat
     5101 tgatgataaa atgcaacagt gttttgttac ttggcagcat agctgctaaa attaaccaaa
     5161 ttacccatcc tgccaacctc agagtggaga tggatatcaa ccccgtggtg accatttaag
     5221 gagggaaaaa gattgtcagt atacaaaatt gttaccgtac cagatcccat tctgagacaa
     5281 aaagcgcaaa aagttgaaaa agtaaatgcc ggcgttcttc gccttctgga caatatgcgt
     5341 gataccatgt acgctgcgga cggaatcggc ttggctgcac ctcaaatcgg tgtttccaag
     5401 cgagtcatcg taatcgatcc cggagatcag ctgattgaaa tgatcaatcc ggaaatcatc
     5461 acccgggaag gcgaacaaac cgcccgggaa ggatgtttaa gtgttcctga cctggttggc
     5521 tggtgcaaaa gggcccaact aataacagtt cgtggcatga accgcgatgg tgaggaaatt
     5581 gaaatggaag ccagcgattt gctggccaga gcatttcaac atgaaattga tcacctggac
     5641 ggaattcttt tcccggacaa agccatacag acccgaaaag aaaaataaac tgacagggaa
     5701 ggagggggtg aattgaaaat cgtttttatg ggtacatcag cgttctcggt accgtctcta
     5761 aaaagactga tggacacaga acatgagatc acaggtgtgg taacccaacc ggatcgtccc
     5821 cgcggacggg gacaaaaact cattccctct ccggttaagg aattggcttt gaatcatcac
     5881 ctggcaatat atcagccgga aaaaattcgc gctgaggaag cgattgatca tgttcgcagc
     5941 ttggatccag atttgattgt ggtggtctcc tacgggcaga tcatcccgcc ggagatactt
     6001 gatttcccca agctgggctg cattaatgtg catgcttcgc tgttgccgcg ttatcgtgga
     6061 gcagccccca ttcaaagggc gattatggcc ggggaaaccg tcagcggtgt aactaccatg
     6121 gccatggatc ggggcttgga tacaggggat attatactgc aattgccggt caaaatcggg
     6181 gcagacatgg attacggtat ttacgctgaa atcctggcca atgccggagc cgacttgctg
     6241 attgatacct tggcgatcat ccgcagcgga tcatatccgc gccgcaaaca ggatagcgcc
     6301 aaggctacct atgcgccaat gattcagcgc gaagaagaac gcattcagtg gcagcaacct
     6361 gcccttgtca ttaataatca gattcgggct tttagtcccc aacccgctgc ttatacatct
     6421 tttaataacc agcgtttcaa ggtgttttct tcgcgcttgc tgaacgagaa caccgctgct
     6481 gccccaggag agattgcgca tatcaatgca tcgggaattg tggtgcaatg cagtgaggga
     6541 atgctggaga ttaaagaagt acagctggca ggcaagaaaa ggatgcccag caatgagttt
     6601 gtccggggat tcaaacttgt tccaggggat gttctgggcg aataaagtgt tgttgtggtc
     6661 tttttcatgg aaagcaggag attgtaagga tggagagtaa aaaacgtatt tatatcggat
     6721 tgttgatcat cagcctggtg acgatggcct tgttcatgcc gctgctggta tttctgctcc
     6781 aacagaatac gccggtcagc aagatcgtgc ttaccgttat cgcgatactc attggcggga
     6841 tattttttct tttggggttg ggcattatgg cgatcattat tatgattatc cgttccaaaa
     6901 ccaatccctc cctggatagt atcgccaagc tggccaatga actacttttt ccaataacgc
     6961 ttgtaatagg aaaagtgctg gctattcctc cggaaaagat aaggtcatcg tatattgcgg
     7021 tgaataatta tttggtaaaa tcgaagcata ttatcttaaa gggtgaaaat gtattgattc
     7081 ttttgccaca ttgtctgcag aatgatgagt gtccccataa aattacgatg gacataaaca
     7141 attgcaaaca atgcggaaaa tgctgtatca gtgatctaat caaacttagc cacaagtact
     7201 cggccatcat caaggtggcc accggtggca ccctggctcg caaaatgatt aaagaatgcc
     7261 gtccttcagg ggtgattgcc gttgcttgtg agagagactt gtcctcgggc attcaggaca
     7321 gtggttcgct tccggtttat ggtgtgttaa accgcagacc caacggtccc tgtcacaaca
     7381 ccagcgtgga tgtagcaaag gtggaacagg ctttaattat gatttgtaga ggaggatgaa
     7441 aagtgggata tttgatttta atactacccg ccctaatact gtcaatttat gcacaattca
     7501 aggttaaatc aacattcagc aaatactcgg ctgtcaggtc gctaaaaggg attaccggtc
     7561 gagaggcggc ttatggcctg ttgcgcaaca atggcataaa tgatgtcgcg attgaagcca
     7621 ttcgcggcag cctgacggat cattatgatc cctcggcaca tgttctgcgg ctttctgaaa
     7681 ccgtgtatgg caatgattcc attgctgcta tcggagtggc agcgcatgaa gtggggcatg
     7741 ccatacagca ccagcaaggt tacaaaccca tggaaatcag acagaaaatt gtacccgtga
     7801 ccaattttgc ttcgaccgcc tcttttccca tcctgttgat tggcatggtg atagccagta
     7861 cagatcttat tatgattggc atcttgcttt tttcagcggt ggtattgttt catttggtta
     7921 cacttccggt ggaatttaat gcttccaatc gtgccgttgt gcaattgcat cagtacggcc
     7981 tgatcagcac ggaagaagtt cccctggtga aaaaagtgct gggtgctgcc gccctgactt
     8041 atttggccgc tgcgctttcc gccatcgcca atttgctcta ttacctggcc atctttgccg
     8101 gttcagatga ttaaacccgt ggggaaatca atgcgtacgc aaaacgacgc tttgctaacc
     8161 gatccgcggg agatggcagt aaccctggtt tatcagatca tggaaaaggg tgcctatgcc
     8221 aacctcagcc tggaccatgc cttgcatggg tcgaaaatgc tgcaaaaaga caaaaacctg
     8281 gtcaccgaac tggtgaacgg cagtatccgt atgattaagc accttgattg ggtgcttgat
     8341 ctgtttctgc gcaagagcat tgacgaacaa aatccctggg tgcgcagtgc tttacgggta
     8401 gccacttatc agattatgtt tatggagcgg attcctgact atgctgcagt ggacagtgcc
     8461 gttgcctttg tccgccgcag tgtaaacaaa acgttggcag gagtatgcaa tgccgtcttg
     8521 cgtaacctaa tcagaaacaa agccgaaata tcctatccgc cggtcaattc accaacttat
     8581 ctggcagttc tccattccca accagaatgg ttggttgaac gttggacagc atgttatggg
     8641 gtgcagctca ccaccgccat gctggcctat atgaaccaga aaccattgct tactttacgg
     8701 gtcaattcct tgcgaaccga agctgctgca ttagccatgg aattatcacg cgagggtacg
     8761 caagtcagac ctggacgact gccgggttcc cttatcatcg actccctgga aaaaagcatt
     8821 gaacaattgc atacatacca ggcaggtggt ttttatctgc aaagcgaagc cgccatgctg
     8881 gcagcagata tactgcatcc cgttgccggg gaccaggtgt tggatttatg cagcggcgta
     8941 ggagggaaag caacccattt tgccgaaaag atgaataacc agggactggt aaaagcggtg
     9001 gaacttcacg cgcacaaact aaccttgctg cagaaaaact gtcagcgttt aggtgtcact
     9061 attgttaccg gtgaacacgc cgatctgctg gcgatggaag aggcgccggt atggccaaaa
     9121 gtcttcctgg acgttccttg ctccgggctg ggcgtattga atcggcgggc ggatgcacgc
     9181 tggcgcaaag accccggaga aatagcggca ctgaccaaat tgcaggcagc catgctggcc
     9241 aaggcaggca aaatgacagc gccgggtggg attctggtct actctacctg caccatcaat
     9301 accgaagaga atgaagagat cgttgttaaa tatttgcaag accatccgca atttcaattg
     9361 caatcgatgg cgcgggaaat ggatcatttt caattggatc agcctgaccg ggaaatggcg
     9421 gaaaagggct ggctgacgat catccccggc aaatacggtt gtgatggaat gttttatgcc
     9481 cgtctgcgaa ggagcaatga ttaaatgaat gaccaaaaca aaacagaact gttgggtatg
     9541 aaccaaagtg aactggaaga atttgtaacc ggcattcatg agccgaagtt tcgtgcccgg
     9601 cagctatata aatggatcta tcaaaaacag acggcttcct tttatgagat gagcgatatt
     9661 cctcgtgacc tgcgctttcg actggatgaa atcgcccaaa tatccatccc cagatttatt
     9721 aaacaaagag tatcccacga cggaacccgc aaatttctcc tggagatgaa tgataaaaaa
     9781 aaggtggaaa cagtcattat cccccaaaac gcgcaaaaaa actcccaata caccgtttgt
     9841 atttccagtc aggtgggatg cccggttgga tgtgtttttt gtgcgaccgg tgcgggtggt
     9901 tttcagcgtc agctgctggc ctatgaaatt gtcggacagg ttttgagtgc ccggcgggaa
     9961 ctggctaaaa gattaaaaag aacggagaac gatttgatca ccaatgtagt ctatatgggg
    10021 atgggtgaac ccttgttaaa ttacgatgaa atgatcaaat cgctttacct gctcaatgaa
    10081 cataaaggca tgaatattgg ccaaagacat attaccattt ctaccgcagg ggaggtcaac
    10141 ggcatccgca aactcatccg ggaaaacctg caggtgacgc tggcggtctc tctgcatgcc
    10201 tgcaacaatg aattaagaaa caccttggtg cccatgaaca aaaagtatcc gctggaaacc
    10261 ctgctggagg ccgtcaaaga gtatgcacag accagcaacc gtcgtgttac ctttgaatat
    10321 gttatgctgg atgaaatcaa catccacaag caagatgccg aggccatgat caggctgttg
    10381 aaaccgatat tggcgaatgt caatctaata ccttataatg aagttgacgg gctaaatttt
    10441 aaaagacccc cccagcatcg catccgccat ttttatcaat ggctgacagc ggggggactg
    10501 aatgttaccg taagggaaga acggggaaca gatatcgaag ccgcctgcgg tcagttggct
    10561 gtgaaaaaag accggcaggt gtagagatat tgaggtgaat tatgaaggca gctgggttaa
    10621 ccgacattgg tttggtgcgc aaaaggaacg aagacaatta ttggctggac acggattatc
    10681 aattgtttgt ggtttgtgac ggcatgggag gacatctggg cggagatatt gcatcccgtc
    10741 tggccattga aaccatcacc agggaactga ttaaggaaga tcttgacgaa tttcctgcag
    10801 ccctggagaa agctgttcag tctgccaatc gtattatatg ggagacagga caggctacgt
    10861 ctgatttgaa tgaaatgggg acgacactga cagcggctgt tatccgggga aattcaatct
    10921 ggatcgcgca tgttggagac agcagtcttt acatcatcca tgatgcacaa atcaccaaac
    10981 cgaccagcga ccataccctg gcggaacaaa tgcgcaaaga tggcttgatt gccaaagatg
    11041 atgagcgtta tcaatctttt caccatgtac ttgtccgcgc cctgggcgtg gaaaaccagg
    11101 tagacattga tatacaccag atggaggtta caccgggaga ctggatcctg ctttgtacgg
    11161 acgggctgag caatctggtg gagcaaacgg aaatcaaagc gttgctggag catgaaaacg
    11221 aaccgcagac ggcctgtcaa aagctgttgg aattggcgtt ggcccgaggc ggatatgaca
    11281 atataaccat tatccttata caaatgtaga tttgggggta tatgttttgg accaaattat
    11341 tcttgggggc cgttacgaat tgctggaaat cataggcgaa ggcggaatgg ccaaggtata
    11401 taaagcccat tgcagaatac tggacagaat tgtggcaata aaagtattaa aagaagaata
    11461 ttcctgcgac cagggttttg tggaaaaatt taaaaccgag gccctgtccg cggcgagaat
    11521 caatcatccc aatattgtaa acatctatga tgttggtcag gaaggcgaca tttactatat
    11581 tgtgatggag ctggtggaag gcaagaccct gaaagaaatc atcagtacgg aagcaccgct
    11641 gatgtttgag cgggcggtgg acattgccat catgatttgc gatggcgtcc atcatgccca
    11701 tgaaaaaggg attatccacc gggatatcaa acctcataat atattaatca ctgaacacgg
    11761 catggtgaaa gtcgctgatt ttggcatcgc ccgggccatc accaatgcca ctattaccta
    11821 tggcaaccaa aatattgttg gctcagtcca ttatatttct ccggaacaag ccaaaggcga
    11881 ggcagtcaac cgcaccacgg atatttattc cattggttgc gtactgtacg aaatgctgac
    11941 cggtcggcct ccctttagtg ccgacagcgc cattaccgta gccatgaaac acatccacga
    12001 cgaaataacg cctccgcgca gatttaatcc ggatatacca gctgcgctgg aaagcattat
    12061 tatgctggcg atgcaaaaga atcccaaaca gcgatttgcc agtgcccagg aaatgcgcaa
    12121 tgcgcttttg agtattcata tgtaccggcc ggaagaacat attcaaaagc gcaaaaaaga
    12181 aaaaataatc accggtaccc cggtcatgtt tgagggagat gaaagcttga aaaaaaagaa
    12241 aaagctcagt ccgctcggga tcgccatcat cgtcattgcc gtgttgggtt tgctgtccgg
    12301 tgcattgctg tcgggcggcg ggttgtttgg caaggaagtg gtcgtaccca atgttgaggg
    12361 catggattac aaactggccg acgaggaatt atccaaagtc gggctggtaa tgagtgtcgt
    12421 cggcaaggaa tacagcaacg aatttgaaaa agacgtggtc atatcccaag accccggcaa
    12481 agaaagaaaa gtcaaagaag gacgtaaagt agaagtggtg atcagccagg gttcggaatt
    12541 gataaccgta cctaatatca cgggtatcga tattcaggac gcagttgtga aactgggcaa
    12601 tgcaggcttg aacatgggag atatagaaaa ggaatatgat gaggaaaaac cggaaggcat
    12661 gatcatttcc caggatccgg cccgaggcag caaagaagaa gccggaacca gtgtcgatgt
    12721 tgttgtcagc caaggcaaac agccggaaaa aatagcggta cctgatttga aaggcttgac
    12781 cctggataaa gcgcggacag cattggatga taaaaaactg gttctgggag aggtcaaacg
    12841 cgaggaaagc acggtctaca tggctgatca tattgtcaaa caggatacag atcctggtgt
    12901 aatggtggaa gaaggaaccc gaatcaatgt ggtgatcagt accggtcccg ggcctcaggt
    12961 aaagaccaaa gtactgcaat tcaagcttcc ccgcgaggag gaaacgatga atgtaaggat
    13021 tgaagtctcc gatctgcatg gtgagcgtca atcatacaat caaatgcacc atggcgacga
    13081 tatcatcaat accagtatca actattacgg tgccggcact gcggaagtat ttatcaacga
    13141 caagtcggtc aagatttaca agctcaacta gccagcggag gggaagatga ccattacgca
    13201 ggagcaaggc ctggtattga aaagttatcg tggattctat tatgtacaag acaaccatac
    13261 ccgaatcatc gagtgtcgtc ctcggggaaa actgaaaacc cgagttctca gcggtgatcg
    13321 tgtcatgttt acgcctttgg aagatgacaa aggcgtattg gaaagcattc ttccccgcaa
    13381 taatgaaatg gttcggcccc gggttgccaa cgtgagcatg gtgctgattg tcatggccta
    13441 cgatcgccca ttgcccagtc tgaccttgct ggaccggttg ctgttattgg tgcaatacag
    13501 tcaattgcaa ccctgcatag tcctcaataa atgcgattta cctgtccatc agaccgtgac
    13561 cgaaattttg gattactatc cacgcgccgg ttatccggtg catagtgtat cagcccgatg
    13621 caatcaaggt atggaagacc tccagcaagc cattcacggg gaaatagctg tattggcggg
    13681 accttccggg gttggcaaat caagtctgct gaatcatctt gccggtctgg atttggtccc
    13741 cacccaggaa gtcagccaaa aaatcggccg gggccggcat accacccgcc atgtggaatt
    13801 atatccgctg gacaaccaaa gctgggtagt tgatactccc ggtttcagtg tattggatat
    13861 gccccggatg cgccgggagg aattgcccgg ttggtttcct gagtttaacc gctttgcgga
    13921 aacgtgcaaa tttgccaact gccttcattt caaggaaaag gaatgcgggg ttaaaaacgc
    13981 agttgaacaa ggcgaaatcc tgacttcccg gtataataat tatttgtcca tgctggagga
    14041 agtaatggaa aatgagaggt gttattagtg gttttagtgg ccccgtcgat tctatccgca
    14101 aactttgcgc agctggggga agattgccag cggcttgaac aagccggtgc ggattggctg
    14161 catgttgatg ttatggatgg acattttgtt cccaacttga ccatcggtcc gcccgtggta
    14221 agggatatcc ggcgcataag ccatttgctg tttgatgttc atctgatgat tgaaaagccg
    14281 gagtcgctga ttcccgcctt tattgatgcc ggagctgacc tgattaccgt gcatgcagaa
    14341 gcctgtaccc acctgcatcg gataattcat atgatcaaaa atgccgg
//
LOCUS       DAOTHZ010000055        13962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5254_length_13962_cov_7.701230, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000055 DAOTHZ010000000
VERSION     DAOTHZ010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5254_length_13962_cov_7.701230"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="P5549_07340"
     CDS             <1..296
                     /locus_tag="P5549_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12923.1"
                     /translation="WIETFLRPRSFITGAVAPRAGAWIETNPACTLRDHQGVAPRAGA
                     WIETEYPFPKAKADSVAPRAGAWIETVDPEQLGCSFSVAPHVGVWIEISYNIT"
     gene            342..1514
                     /locus_tag="P5549_07345"
     CDS             342..1514
                     /locus_tag="P5549_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRW12924.1"
                     /translation="MNIPLHGIKVLDITANLAGPFCTQILGDFGAEIIKVEDIRGGDP
                     SRVNPPHLGEEGANFFVVNRNKKSLALNFHSDEGKKLYKELAAQSDVVVDSFRPGYMK
                     KLGLDYDSLKSINPRLIYCSMTAYGQEGPMANAASHDVNILSLAGITGLTGDGTEKPQ
                     LTGAQLGGLAGGSLNGVIAILLALLYRNRTGQGQFCDVSMLDGSLAFLGNVLGFQSGF
                     GEEVRVFSGQTAFYNIYETKDGGYISLGAIEHKFWKEFCEAIGRPDLLATHWDISSQE
                     SMKQEIAAIMLTRDRDEWMQIFSGTDCCVTPVISMDEVCEQAWVKEREMIYTLPDFRK
                     SGMELKMVGQPIKLSATPAQVKAEFPRLGEHSEEILAWLGYAQEEIERLKLQGIVG"
     gene            1597..1740
                     /locus_tag="P5549_07350"
     CDS             1597..1740
                     /locus_tag="P5549_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12925.1"
                     /translation="MDDRKKAWLLLLGETPGPMVLAGGLIVVAAVTIRCIAAISEENA
                     TTS"
     gene            2112..3383
                     /locus_tag="P5549_07355"
     CDS             2112..3383
                     /locus_tag="P5549_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HRW12926.1"
                     /translation="MLYFWLSIGIFLITYAFIIADKIHRTIVVWIGAVLVIALGVINQ
                     EKAIEHVDFNTLLLLIGMMVIVGITRKSGVFEYLAIKSAKMVKGQPVALLVVLALITA
                     VASALLDNVTTVLLIVPVTYALADRLEVSPMPYLLTEIIASNIGGTATLIGDPPNIMI
                     GSATGLGFMDFIVNMGPPAIIVLAVTILILVAFFKKDMGARQDKIDRLLELCETDSIR
                     DWMLLKKSLFILALTMLGFLLHQQLHLESATVALFGAGLLMLVSREEPEEVLFAVEWP
                     TIFFFAGLFILVGALQVNGVISLIAQQSLQVTGDNVMITGTLILWLSAVASAFVDNIP
                     FVATMIPLLQEVGQLSQMPMDSLWWSLALGACLGGNGTLIGASANVIVAGMAERNGTP
                     IKFVDFLKLGFPLMLLSVLISNIYLFLRFWY"
     gene            complement(3449..4579)
                     /gene="budA"
                     /locus_tag="P5549_07360"
     CDS             complement(3449..4579)
                     /gene="budA"
                     /locus_tag="P5549_07360"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HRW12927.1"
                     /translation="MQKTVTAFLLAFICSLLLVVPAAAESPVTVNLNGQPVSWSPRPE
                     IADGLTMVPLLPLGEALGAKTEWIPSRQAASIDLPGVHMEITAHSSAAMVNGEKISLP
                     VAPVIKDGEIVVPLRAVAEVLKIPVQWDSTTWTVHLHSGAARDTVHQISLLSSLLEGH
                     YDGLTTFRELKNYGDTGIGTFAALDGEMIALDGQFFQVKADGIAYSVTEEMSTPFACV
                     TYFEADQAADIVEPVDFSQFQAYVDNMISNKNLFYAIKATGSFSHVQTRSVPAQQKPY
                     PPLVDVTKNQPTFDLENLNGTLIGFWCPAYVDGLNVPGYHIHFLNADKNRGGHLLDFT
                     MESGRIEIDVTPNFFMRLPDSDEFGQIDMRKDRAEETSAVEK"
     gene            4860..6737
                     /gene="htpG"
                     /locus_tag="P5549_07365"
     CDS             4860..6737
                     /gene="htpG"
                     /locus_tag="P5549_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRW12928.1"
                     /translation="MAKKQFKAESKRILDLMINSIYTNKEIFLRELISNASDAIDKIY
                     YKTLMDENLSFEKESYFIKIYLDKESRTLRISDTGIGMTREELDDNLGVIARSGSLNF
                     KKENEMSDGYDIIGQFGVGFYSAFMVADTITVISRAFGSEEAFKWESKGIDGYTIEPC
                     EKEGTGTDIILKIKAGTEEEKYDEYLEEYRLRSIIKKYSDFIRYPIKMDVSRSRLQEG
                     SEDEYEQYTEEQVINSMVPIWKKNKNELTAEDYENFYFEKHYGFDTPISHLHISTDGT
                     VSYNAILFIPEKMPFDFYTREYEKGLELYSNGVLIMNKCADLLPDYFSFIKGMVDSED
                     LTLNISREVLQHDRQLKLIAKNIKTKIKNELNRILQDDREKYETFFASFGRQLKYGVY
                     DNYGVDKDFLQDLLIFYSSREKKMVTLAEYVSRMPEEQKYIYYAAGESVEKLDKLPQT
                     ELLADKGYEILYFTDDVDEFAIRIMMNYQDKDFKSVSGGDLGLDNDNETDAEAAEEHK
                     NLYAAMKEMLGSKVHDVRVSKRLKNHPVCLTNEGEISIEMEKVLNAIPTNEQKIKAAK
                     VLEINPGHEVFAALLEAYENDKDKLALYTDLLYNQALLIEGLPLEDPLQFADNICKIM
                     K"
     assembly_gap    6857..6956
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6957..7405)
                     /locus_tag="P5549_07370"
     CDS             complement(<6957..7405)
                     /locus_tag="P5549_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12929.1"
                     /translation="MSYLQNTQLELFSFLVCAILLFDILLRKEKYHFDNKLFLTVLIA
                     NMLILLIDALGWLIEGNLLDPLNAIYLRLIFIYFMMNPILPYLLLLYTDFQIFQDRHR
                     LRKLFWPYLTPMILIAILLIANPFTQVLFHIDQNNVYHRGPLFNLFL"
     gene            7589..8161
                     /locus_tag="P5549_07375"
     CDS             7589..8161
                     /locus_tag="P5549_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRW12930.1"
                     /translation="MMKELILKNRSYRRFQQERPVADEVLRELIDLARLSPTGHNRQA
                     LRYFISNQQDMNEKIYSCLAWAGYLKDWGGPPPGERPAAYIVMLKDGSVGAALTQDQG
                     FAGQSILLGAVEKGLGGCFLVNVGKQQLTEFLALDEKYEIAAVIALGYPKEKVVLDPL
                     ELYGDVKYWRDDEQVHHVPKLSLDDIILNK"
     gene            complement(8237..9910)
                     /locus_tag="P5549_07380"
     CDS             complement(8237..9910)
                     /locus_tag="P5549_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12931.1"
                     /translation="MKYLPEERNWEKEGVWPDFWPKYFEHGNMPIVWHLHLNALRHPK
                     KTAVIFYGTRISYKELEQLVLHAAGGLKKLGVEKGDRVYLGMQNCPQFIISYYAAMAV
                     GGVIIGGSPMFKKGEMSYILNDCGAKVAIIEEDNYPIFQSIRDQVPVESVVVTSLNEF
                     VPDNPEIPVPPGMVVEGLDYSGAIDWKDFIQSDPVGKYESLDIDKDLACLQYTSGTTG
                     VPKGAMLTHRNILVNAIQLPIILGGHADSVYGAVVPIFHITGMQNNMNGPIFLGATIV
                     LFTRFDTEVFLQASELYKITSWVAITTMNIAVINHPTLNKYDLSSYEAIGTGGAPVPA
                     ELIKRYKDQFGLDLIDGWGMSETASAPLASPAGHVKAGSCGIVVRGFDVRVADLTDPT
                     KNVPRGQEGEMWMRGPSIAREYWHQPPEAQAESFKEEGWLATGDVIIMDEDGYIFIVG
                     RTKEMIKASGYSVFPAEVEKYLYQHPAIKECCIIGVPHEYRGEDVKAYIVLKDDYKGK
                     ITAEEIIDWAKDQMSVYKYPRQIEFRDDLPKSATGKVMRKILKEELLNK"
     gene            complement(10221..10928)
                     /locus_tag="P5549_07385"
     CDS             complement(10221..10928)
                     /locus_tag="P5549_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12932.1"
                     /translation="MLKINDLQVFYGGIHALKGISLQVPENKIVTLVGANGAGKSTLL
                     RAVSNLVPVASGNITFKDQEITGKPTVSIVKSGITMVPEGRRIFANLTVLENLKMGAY
                     ARKDKAGIEADTEKMYGLFPRLKERSWQVAGTLSGGEQQMLAIARALMSRPQLLMMDE
                     PSLGLAPLLVKEVFRIIKNIHSQGMTILLIEQNATAALKIADYGYVLETGTIVMEGPG
                     QELANNQAVRQAYLGKS"
     gene            complement(10915..11700)
                     /locus_tag="P5549_07390"
     CDS             complement(10915..11700)
                     /locus_tag="P5549_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW12933.1"
                     /translation="MNTCTLRTDHITMEFGGLRAVSELSLEVAPGTIVGLIGPNGAGK
                     TTVFNMITGVYKPTSGRIYFQDRDITGMKSDKITALGIARTFQNIRLFQSMSVLENVM
                     ISKHVRLQANLLEAVLGLPRYTRANHANYDECMDLLKKVQLDDVCHEAATSLPYGKQR
                     RLEIARALATNPSLLLLDEPAAGMNPAESLELMDFVARIRSEFNITVLMIEHHMEVVM
                     GICEKIYVLDYGETLAKGNPAEIQNDPKVIEAYLGAVDIDAEN"
     gene            complement(11697..12761)
                     /locus_tag="P5549_07395"
     CDS             complement(11697..12761)
                     /locus_tag="P5549_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW12934.1"
                     /translation="MEKNKRTKNILTILTLIVVCGLLYWSENNLNNFYIRVLNLAAIF
                     AILGLSMNLVLGFTGMFSLGHSGFMCVGAYTAAILTMSPASKELIYYMVPINPALAQI
                     EWPIFPAIVAAGLMAALFGLLVGIPALRLRDDYLAIATLGFAEIIRIVVTNTTSITNG
                     SLGLKNIPSINSIWLYWGAAILTLIILKRMINSSWGFAFRAIRDNEIAAEAMGVSIFK
                     HKLISFTIGAFFAGIAGALMGFLITSVDPTMFRFLFTFQILLIVVLGGMGSLTGSVIS
                     AIVITIMMEVLRFVEQPISILGLNIPGLSGMRMVIFSVLLLMVILFYRQGLMGTKEFN
                     WDWVLRKFNRIKAGRRGETA"
     gene            complement(12765..13658)
                     /locus_tag="P5549_07400"
     CDS             complement(12765..13658)
                     /locus_tag="P5549_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW12935.1"
                     /translation="MSMDIFLQNIANSITLGSLYALIAIGYTMVYGILRLINFAHADL
                     LMVASYIAFYGILIFTLPWYIAFAIAIILTTLLGIGIEKAAYRPLRNAPRISVLISAI
                     GVSFLLENIGIVAIGARPKSFPRPEELVWNIQLGNISFLSLTIIIPVVTLLLLVAVTL
                     MVKKTKIGMAMRAVSRDFETSSLMAVNVNKVITATFAVGSGLAAIGGLMWSMQYPQVD
                     PLMGVFPGLKCFIAAVMGGIGSIPGAMLGGFVLGFCEVMLVGFFPGVSGYRDAFAFVI
                     LILILLFRPGGILGENVVEKV"
     gene            complement(13776..>13962)
                     /locus_tag="P5549_07405"
     CDS             complement(13776..>13962)
                     /locus_tag="P5549_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRW12936.1"
                     /translation="AITRADTADSVKIRDALAQTKDFQGATGIITLDENRDATKTAII
                     KKVADGKFVFEGKVDPK"
BASE COUNT         3607 a         3213 c         3563 g         3479 t
ORIGIN      
        1 cgtggattga aacttttttg cgccccaggt cgtttattac cggcgctgtc gcgccccgcg
       61 cgggcgcgtg gattgaaaca aatcctgcct gcactctacg ggatcatcag ggtgtcgcgc
      121 cccgcgcggg cgcgtggatt gaaacagaat atcctttccc taaagcaaaa gccgattctg
      181 tcgcgccccg cgcgggcgcg tggattgaaa ctgtcgatcc cgagcagctc ggatgctcct
      241 tctctgtcgc gccccacgtg ggcgtgtgga ttgaaataag ctataatatt acttaaataa
      301 gttagaaagt taggtgcctg gcaccgtagg gagaatgctt gatgaatata ccgctccatg
      361 gaattaaagt tttagatatc actgcgaatc tggccgggcc tttctgcacc cagatcctgg
      421 gggattttgg cgcggagatt atcaaggtgg aagatattcg cgggggtgat ccttcccgtg
      481 tcaacccgcc gcatttgggg gaggaagggg ccaatttctt tgtggtcaat cgcaataaaa
      541 agagcctggc cttaaacttc cacagtgatg agggcaaaaa gctgtataaa gaattggcag
      601 cgcaatctga tgtggttgtg gacagctttc gccctggcta tatgaagaag ctggggcttg
      661 attatgatag tttaaaatcc atcaaccctc gtcttatcta ttgttccatg accgcctacg
      721 ggcaggaagg tcccatggcc aatgcagcca gccatgatgt caatatacta agtctggccg
      781 gcatcaccgg tctcaccggt gacggaacgg aaaaaccgca attgaccggt gcgcagctgg
      841 gcggtctggc gggggggagc ttaaacgggg tcattgcgat tcttctggcc ttgctctatc
      901 gcaatcggac tggccagggc cagttctgtg atgtatccat gctggacggt tcgctggcat
      961 ttctgggcaa tgtactgggt tttcaatccg gattcgggga agaagtccgg gtgttttccg
     1021 gccaaactgc tttttataac atttatgaga ccaaagacgg cgggtacata agcctggggg
     1081 ccatcgagca taaattctgg aaggaattct gtgaggccat cggcagaccg gatttgctgg
     1141 caacgcactg ggacatttct tcccaggaga gcatgaagca ggagattgct gccatcatgt
     1201 tgacccgcga ccgggacgaa tggatgcaga tcttctccgg gacagattgt tgtgtgacgc
     1261 cggtcatttc gatggatgaa gtatgtgaac aggcatgggt caaagagcgg gagatgatct
     1321 atacacttcc tgactttcgt aaatccggta tggaactgaa gatggtcggt cagcccatca
     1381 aattgtcggc cacccctgcc caggtcaaag cggagtttcc gcgactgggt gaacacagtg
     1441 aggagatcct cgcatggctg ggctatgccc aggaggaaat tgaacggttg aagctacaag
     1501 gcattgtcgg ctaggccaac tcgatttggt tcttgtaaaa tactgtcgcc atcattatag
     1561 tagatatgga tgtttgcgaa acaggaaatt gaaagcatgg atgacaggaa aaaggcctgg
     1621 cttttgctgc tgggggagac gcctggcccc atggttttgg ccgggggatt gattgttgtc
     1681 gcggcagtca cgattcgctg tattgcggca atatccgagg aaaatgcgac cacgtcttga
     1741 gttgtcaggc atttattgtt atgataatat tcaggtgaca ttgaaatata taaacgacgg
     1801 tcaaagctgt ggcgatgaaa atcgttacgg cggggatgaa acacctgggt tcccggtggg
     1861 agaatatggt tttttatccg tgagaaatcg gcttactggg gtgggtagcc tgctggacag
     1921 gtgtacctac ttttttttgt cggttttttt gacaggaggt gaataatact atgaaagaac
     1981 tcgcgaaaca agttaaaatg gaattaattc ggactttgtt ctggctggtg gttgccctgt
     2041 cggtttccat cgggctgtat tttatgattt ggtaaagcca ggtcaggacc aattggcggg
     2101 gggatgaata tttgctttat ttttggcttt cgatagggat ctttttaatc acgtatgcgt
     2161 ttattattgc tgacaagatc caccgtacca ttgtagtttg gatcggtgca gtcctggtca
     2221 ttgcgctggg agtcatcaat caggaaaagg ccatcgagca tgttgatttc aatacgcttt
     2281 tattgttgat tggcatgatg gtgatcgtgg gtatcaccag aaaaagcggt gtctttgaat
     2341 acctggccat caaatctgcc aaaatggtca aagggcagcc ggtagctctg ctggtggtgc
     2401 tggcactgat caccgcggtt gcttccgctt tattggacaa tgtgaccaca gtgcttttga
     2461 ttgtgccggt cacgtacgcg ctggctgacc gtctggaggt atcgcccatg ccttatctgc
     2521 tgacggaaat cattgcgtcc aatatcggcg gtacggccac actcatcggc gatccgccca
     2581 acatcatgat cggcagtgcc accggcttgg gctttatgga tttcatcgtc aatatgggtc
     2641 cgccggctat catcgtgctg gcggtgacaa ttttgattct agtagccttt tttaaaaaag
     2701 acatgggagc ccggcaggat aagatcgatc ggctgctgga actctgtgag acggacagca
     2761 tcagggattg gatgctgctc aagaaatcgt tgtttatcct ggcgcttacc atgttgggtt
     2821 ttctgcttca tcagcaattg catttggaat cggctaccgt tgcgctcttt ggagcggggc
     2881 tgctgatgct ggtctcccgg gaggagcccg aggaagtgct gtttgccgtg gaatggccca
     2941 ccattttctt ttttgccgga ctcttcattc tggtaggcgc cctgcaggta aacggcgtaa
     3001 tcagtctgat tgcccagcag tcgctgcaag tcaccggaga caatgttatg atcaccggga
     3061 cacttatttt atggctgtcg gcggtagcgt cagccttcgt ggacaatatt ccttttgtgg
     3121 cgaccatgat ccccttgctg caggaagtgg ggcagttgtc acagatgccg atggattcac
     3181 tgtggtggtc gctggccttg ggcgcctgtc tgggcggcaa cggcaccttg atcggggctt
     3241 cggccaatgt cattgtggct ggcatggcag aaagaaacgg gacgccgatt aaatttgtgg
     3301 atttcctcaa gctcggcttc ccgctgatgc tattgtccgt attgatctcc aatatctatc
     3361 ttttcctgcg cttctggtat taatcaaggc gccgggcatt acgcaggagc aaccgttttt
     3421 ctggtttcga ccaggaggag ggttgctttt atttttctac cgcactggtt tcttctgctc
     3481 tgtctttgcg catgtcaatc tgtccgaatt cgtcactgtc aggcagacgc ataaagaaat
     3541 tgggggtaac atcgatttcg atccggccac tttccatggt gaagtcgagc aagtgtccgc
     3601 cgcgattctt gtcggcgttc aggaaatgta tatggtaacc cggcacattg agtccgtcca
     3661 cataggcggg acaccaaaag ccgatcagcg ttccattcag gttttccaag tcaaaggtag
     3721 gttggttttt ggtcacatcc accagaggcg gataaggctt ttgctgggcg ggaacacttc
     3781 tggtctgcac atggctaaag ctgccggttg cttttatggc ataaaacagg tttttgttgc
     3841 tgatcatgtt atccacataa gcttgaaatt gggaaaaatc cacaggctca acgatatctg
     3901 ctgcctggtc ggcttcaaaa taggtgacgc aggcaaaggg agtgctcatc tcttccgtga
     3961 ccgaataggc gatgccatcg gctttgacct gaaaaaactg tccatccagg gcgatcattt
     4021 ctccgtccaa cgcggcgaag gtgccaatgc cggtatcgcc gtaatttttc aattcccgga
     4081 aggttgtcag cccgtcataa tgaccttcga gcagactgct caacagcgat atctggtgga
     4141 ccgtatcgcg tgccgcacca ctgtggagat ggactgtcca cgttgtactg tcccattgaa
     4201 ccgggatctt caggacttcg gcaacagcac gcaggggtac gacgatttca ccatctttga
     4261 tgaccggagc caccggcaag gatatttttt cgccgttgac catcgctgct gaagaatgcg
     4321 cggtgatttc catatggact cccggcagat cgatggaggc agcttgacgg gacggaatcc
     4381 attctgtttt tgcccccaaa gcttccccca gcggcagtag gggcaccatg gtcaaaccat
     4441 ctgcaatttc cggtcgggga gaccaggaaa ccggctgacc gttcaggttg acggtgaccg
     4501 gggattcagc tgcagccggc acaaccaaga gcagggaaca gatgaaagcc agtaagaagg
     4561 cagttactgt tttttgcatg agttgctcac tccctcgttt tttccatttc atttgtgttg
     4621 tttcacattc tttattgtaa agcatcaagg gaagaacgcc aaccagggtc agctgcattt
     4681 tgcattcccg gttgacatcg gcacgaatga tccgtggtaa aatactgaaa cctattgacg
     4741 atggcgggca aaatgagtaa gataatagat atcttagcac tcgacgagag agagtgctaa
     4801 caactttcta tattaatccc gctaaattat ttagcttttg tttgagagga gcgatgggtt
     4861 tggccaagaa acagtttaag gcggagtcta aaagaatttt ggatctgatg atcaattcca
     4921 tttataccaa caaggaaatc ttcctgcgtg agctcatttc caatgccagc gatgccattg
     4981 acaagatcta ctataagacg ctgatggatg aaaacctgag ttttgaaaag gaaagctact
     5041 ttataaagat ctaccttgac aaggaaagcc gtaccctgag gatttccgac accggtattg
     5101 gtatgacccg ggaagagctg gatgacaacc tgggggtcat cgcccgcagc ggttctctga
     5161 attttaaaaa agaaaacgaa atgagcgatg gctacgatat tatcggccag ttcggggtgg
     5221 gcttctattc ggcttttatg gtggccgata cgattaccgt catcagccgg gcatttggca
     5281 gcgaagaggc ttttaaatgg gagtccaaag gcattgacgg atacaccatt gaaccctgcg
     5341 aaaaggaagg gaccggcacg gacatcatct tgaaaatcaa agccggtacc gaggaagaga
     5401 aatacgacga gtatctggaa gaataccgcc tgcgttcgat tattaaaaaa tactccgact
     5461 tcatccgcta tccgatcaaa atggatgtct ccagaagccg cctgcaggaa ggcagtgaag
     5521 atgaatacga gcagtatacc gaggagcagg tcatcaacag catggtgccc atctggaaaa
     5581 agaacaagaa tgagctgacg gcggaggatt acgagaattt ctattttgaa aagcactatg
     5641 gctttgacac cccgatttcc cacctgcata tcagtacgga cgggacagtc agctacaatg
     5701 ccattctctt tatcccggaa aaaatgcctt tcgattttta tacccgggaa tatgaaaaag
     5761 gtctggagct ttattccaac ggggtcttga tcatgaacaa atgtgccgat ctgctgccgg
     5821 actattttag tttcatcaag ggcatggtcg attcggagga tctgacctta aatatttccc
     5881 gtgaagtgct gcagcatgac cggcagctaa aactgattgc caaaaacatc aaaaccaaaa
     5941 tcaaaaacga actcaaccgc attctgcagg atgaccggga aaaatatgaa accttctttg
     6001 cttcctttgg ccggcaattg aagtacggcg tttatgacaa ttatggcgtc gacaaggatt
     6061 ttctccagga cttgttgatt ttctattctt ccagggagaa aaagatggtg acgctggcgg
     6121 aatacgtgag ccggatgccg gaagagcaga aatatattta ctatgcggcc ggggaatcag
     6181 tggaaaaact cgacaaactg cctcagaccg agctgctggc cgacaagggt tatgaaattc
     6241 tgtattttac cgatgatgta gatgaattcg ccatcaggat catgatgaat taccaggaca
     6301 aagatttcaa atcggtatcc gggggggacc tgggactgga caatgacaac gaaaccgacg
     6361 ctgaggcggc agaagaacat aagaacctgt atgcggcgat gaaggagatg ttgggcagca
     6421 aagtccatga tgtcagagtt tccaaaagac tgaaaaacca tcccgtctgt ttgaccaatg
     6481 agggtgagat cagcattgag atggaaaaag tactgaatgc cattcctacc aatgaacaaa
     6541 agatcaaagc tgccaaggtg ctggagatca accctgggca tgaagtcttt gctgccctgt
     6601 tggaggcata tgaaaatgat aaagacaagc ttgcgcttta caccgatctt ttatataatc
     6661 aggccctgtt aatcgaaggt ttacccctcg aagacccgct gcaattcgct gataatatct
     6721 gcaaaatcat gaaatagcaa cggcccgtgt cagtttcccc acctttggcc gggttgttgg
     6781 ggctgaagag ggaagcagag gatagggcgt gaaaaaagac cgcgcatgag tcgtatctga
     6841 tacgcttatg aagcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa
     6961 gaaacaagtt aaacaagggc ccgcgatgat aaacattatt ttgatcgata tggaacaaca
     7021 cctgggtgaa aggattggcg atgagcaaga tggcaatcaa aatcatcggc gtcaagtaag
     7081 gccagaataa tttgcgcaat cgatgccgat cctgaaatat ttggaaatca gtgtataata
     7141 acaataaata cggaaggatc ggattcatca taaaatagat gaaaatcagg cgcaagtaga
     7201 tcgcatttaa aggatccagt aggttgcctt cgatcaacca tcccagtgcg tcaatcagca
     7261 gaatgagcat gttagcgatt aaaacggtta aaaacagttt gttatcaaaa tgatattttt
     7321 ctttgcgcag gagaatatca aacagcaaaa tcgcacagac caaaaaggag aatagttcta
     7381 attgggtgtt ctggagataa gacaaagaaa tcatcaccct cagatacaaa attttagtaa
     7441 aaacattata attatactac actaaccttt gcggcatgtt acatatttta aataatcaac
     7501 cctcaagtga aatgttggta acaaggcaag gcaaagtttt atgagagaag cagtgtttga
     7561 atagaatact tgaaagtggt gatcaaccat gatgaaagaa ttgatcctca aaaatcgctc
     7621 ctatcgaagg ttccaacagg aaaggccggt ggcagatgaa gtgctgcgtg aattgatcga
     7681 tttggcccga ctgtcgccga ccggtcataa ccgtcaggca ttaagatatt ttatatccaa
     7741 tcagcaggat atgaatgaaa agatttattc ttgtttagcc tgggccgggt atctgaagga
     7801 ttggggcgga ccaccgccag gggaacgtcc tgccgcctat attgttatgc tgaaggacgg
     7861 ctcagtagga gcagctttaa ctcaggatca aggttttgcc ggtcagagta tcctgttggg
     7921 ggcagtggaa aaagggttgg gcggatgttt cttggtaaat gtgggaaagc agcaactgac
     7981 cgagtttctt gcacttgatg aaaaatatga gatcgccgcc gtgattgctt tgggataccc
     8041 caaggaaaaa gttgtgctgg atccactaga gctgtacggg gatgttaaat actggcggga
     8101 tgatgagcaa gtccaccatg tccccaaact cagtctggac gacataattc taaataaata
     8161 aaccagatat cctccagatt tatcagttac ggacaaaaac aaagctcggg gctgcgaccc
     8221 cgagctagca atgatattat ttattcagaa gttcttcttt aagtattttg cgcatgactt
     8281 tcccggtagc gctcttgggc aggtcgtcgc ggaattctat ctggcgcgga tatttataaa
     8341 cggacatctg atccttggcc caatcaatga tttcttctgc cgttattttg cctttataat
     8401 cgtccttcag tacgatatag gctttgacat cttctcccct atattcatgg ggaacgccga
     8461 ttatacaaca ttctttgatg gccggatgct gataaagata tttttccact tcggccggaa
     8521 aaacgctgta accggaggct ttgatcattt ccttggtcct gccgacgata aaaatataac
     8581 cgtcttcatc cataattatc acatcgccgg tggccagcca cccttcttct ttgaagcttt
     8641 ccgcctgggc ttcgggcggt tgatgccaat attcacgggc aatggatggg cccctcatcc
     8701 acatttcacc ttcctggccg cgcgggacgt tcttggtggg atcggtcaaa tccgctaccc
     8761 gaacatcaaa gccgcgtacc acaatgccgc agcttccggc tttgacatgg ccggctggac
     8821 tggccagcgg tgctgaagcg gtttcgctca tcccccagcc atcaatcaaa tccagaccaa
     8881 actgatcctt ataacgtttg atcagctcgg ccggaaccgg ggcaccgccg gtgccgatgg
     8941 cttcatagga agacagatca tatttgttga gggtgggatg attgatcacg gcaatattca
     9001 tggtggttat cgctacccag gaagtgattt tataaagttc ggaggcttgc aggaatactt
     9061 cggtgtcaaa acgggtgaac aagacgatgg tggcaccgag gaaaatagga ccgttcatat
     9121 tgttctgcat gccggtaata tggaagatcg gaacgactgc accatagaca ctgtcagcat
     9181 gaccgcctaa aataatgggc agctggatgg cattgaccag aatattgcga tgcgtcagca
     9241 ttgcgccttt cggcactccg gtcgtgcctg aggtatattg caggcaggcc aggtctttat
     9301 cgatatccaa agactcatat ttccccaccg gatcgctctg gatgaagtct ttccagtcga
     9361 tagccccgct gtaatccaga ccttcgacca ccattcccgg tgggacaggg atttccggat
     9421 tatccggcac aaattcattt agtgacgtta ctacgaccga ttctaccggt acctggtcgc
     9481 ggatactctg gaaaatagga tagttgtctt cttcgatgat ggctaccttg gcgccgcaat
     9541 cattgaggat ataactcatc tcgccctttt taaacatagg gctgccgcca atgattactc
     9601 cgccaacagc catggcggca tagtaggaaa taataaactg gggacagttt tgcatgccga
     9661 gatagacccg gtcacctttt tccaccccta gtttttttaa tccgccggca gcatgcagaa
     9721 ccagttgttc caattcctta tagctgatgc gggttccgta aaagatgaca gcggtttttt
     9781 taggatggcg caacgcattg aggtgtagat gccatacgat tggcatgttg ccatgctcga
     9841 aatacttggg ccagaaatct ggccaaaccc cttctttctc ccagtttctt tcttcaggca
     9901 aatacttcat caaatctccc gtcctttcaa tattaataaa atattttagc aagtccgaat
     9961 ggaaacgcag taactaatcc gacttgttgc tgtctttagg aaacagggtt tgcataatca
    10021 ctaattgtat tatataggat ttcagggggg gtatcactta actggggtaa aaagtgaatg
    10081 atgcagcggc agaaaaaatc agggcacaac cccgacccaa gcagaaagtc gggttgcaaa
    10141 tccatgaaaa aaggcagaaa aaatgctggt tatggttttt attccataac cagcattaaa
    10201 acatttttaa gaaatatgga tcaagacttg cccagataag cctgccggac agcctggtta
    10261 ttagccaatt cctgaccagg tccttccatg acgatggtac cggtttccaa tacataaccg
    10321 taatcggcaa ttttcagtgc agccgtggcg ttttgttcga ttaatagaat ggtcattccc
    10381 tgactatgaa tatttttaat gatccggaag acttccttaa ccagtagggg ggccagtccc
    10441 agcgaaggtt cgtccatcat cagcaattgc ggtctggaca tcaatgcgcg ggcgatggcc
    10501 agcatctgct gttctccgcc tgacaaagtc ccggcaacct gccagctcct ctccttcaga
    10561 cggggaaaca acccatacat tttttcggtg tcggcttcga tacctgcctt gtctttgcgg
    10621 gcataagctc ccattttcag attttccagc acggtcagat tggcaaagat gcgacgccct
    10681 tccggcacca tggtaatgcc gctttttacg attgataccg taggtttgcc ggtaatctcc
    10741 tgatccttaa aggttatatt accggacgcc accggcacca gattgctgac tgctctgagc
    10801 aaggtgcttt ttcctgcccc atttgctcct acaagggtca cgatcttgtt ctccggaact
    10861 tgcagggaaa tccctttcaa tgcgtgaata ccgccataaa atacctgtaa atcattaatt
    10921 ttcagcatct atatccaccg ccccaagata agcttcaatc actttgggat cattttgaat
    10981 ttctgccgga ttgcctttcg caagggtttc accgtaatcc agcacgtata tcttctcaca
    11041 gattcccatg acgacttcca tatgatgttc gatcatcaaa acagtaatgt taaattcgct
    11101 cctgatcctc gcaacaaaat ccattaattc cagtgattca gccggattca tgccggcagc
    11161 cggttcatcc agcagcaaga ggctggggtt ggtggccagg gcgcgggcga tttccagacg
    11221 ccgttgtttg ccgtagggca gggaagtcgc cgcttcatgg cagacatcat ccaactggac
    11281 ttttttcagt aagtccatgc actcgtcata gttggcgtga ttggcccggg tatagcgggg
    11341 cagccccagg acggcttcca gcaaattggc ctgcagtctg acgtgtttgg agatcatgac
    11401 gttttccagc accgacatcg actggaacaa gcgaatgttc tgaaaggtac gtgcgattcc
    11461 cagcgcggtg atcttatcgg atttcattcc ggtgatatcg cggtcctgga aatagatcct
    11521 gccggaagtc ggtttgtata caccggtgat catattgaaa acggttgttt taccagcgcc
    11581 gttgggacca atcagcccca cgatagtgcc cggagcaacc tccagggaca gttccgaaac
    11641 ggcgcgcaaa cctccgaatt ccatggtaat gtgatcggtt cttaatgtac atgtgttcat
    11701 gcggtttccc cccgcctgcc ggcttttatc cgattaaatt tacgtaaaac ccaatcccag
    11761 ttgaattcct tggtacccat cagaccctgg cgatagaaga ggatgaccat cagcagcaga
    11821 accgaaaaga tgaccatgcg catgcctgat aaaccgggga tgtttagtcc taggatactg
    11881 atgggctgtt ctacaaagcg cagcacttcc atcataatcg taatgacgat agccgagata
    11941 actgaaccgg tcaagctgcc catgccgccc agcacaacga tcaagaggat ctggaaggta
    12001 aacaggaagc ggaacatggt gggatcaacg gaagtgatca gaaatcccat cagcgctccg
    12061 gcaatgccgg caaaaaatgc cccaatggta aaggaaatga gtttatgctt gaaaatactg
    12121 acgcccatgg cttcagcagc tatttcatta tcgcgaatgg cccggaaggc aaaaccccag
    12181 ctgctgttga tcattctttt taaaatgatc agcgtcagga tggctgcgcc ccagtataac
    12241 cagatactat tgatggaagg gatgtttttc aatcccaatg atccgttggt tatggaggtg
    12301 gtgttggtca caacgatacg aatgatttcc gcaaatccca gggtggcgat cgccagataa
    12361 tcatcccgca aacgcagtgc cggaatgcct accagcaaac caaagagagc cgccatcagt
    12421 ccggcggcga caatggccgg gaagatgggc cattcgatct gcgccagggc gggattgatc
    12481 ggcaccatgt aatagatgag ttccttgctg gcgggagaca tggttaatat cgcggcggtg
    12541 tatgcaccca cacacataaa tcccgaatga cccagggaaa acatgccggt aaaacccaat
    12601 accaggttca tgcttaagcc gagaatggca aagatggcag ccagattgag cacccggata
    12661 taaaagttgt tgaggttgtt ttcggaccaa taaagcaggc cgcacaccac gatcagggtc
    12721 aggatggtaa ggatgttttt tgtgcgtttg tttttctcca tcgattacac cttctctaca
    12781 acgttttcgc ccagtatgcc gcctggtctg aaaagtaaaa tcagaatcag gatgacgaag
    12841 gcgaaagcat cccgatatcc cgaaacaccc ggaaagaaac cgaccagcat aacctcgcaa
    12901 aagcccagca caaaaccgcc caacatggcg ccggggatgc tgccgatgcc gcccatgacc
    12961 gcagcgataa agcattttag accggggaaa acgcccatta acgggtctac ctgcggatac
    13021 tgcatgctcc acattaaacc gccgatcgcc gccaggccgg agccgactgc aaatgtagcg
    13081 gtaatgactt tattgacatt aacagccatc aggctggaag tttcaaagtc gcgcgacaca
    13141 gcccgcatag ccatgccgat cttggtcttc tttaccatga gggtaacagc aaccaacaac
    13201 aaaagcgtga ccaccgggat gatgatggtc agggaaagaa aggatatatt gcccagttga
    13261 atgttccaga ccagttcctc cggtcggggg aaagatttgg gacgagcgcc gatggcaacg
    13321 ataccgatgt tttccaataa aaaggaaacg ccgatggccg agatcagaac cgagatacgc
    13381 ggcgcattac gcaaaggccg gtaagccgct ttttcgatcc caatgcccag cagggtggtg
    13441 agaataatgg cgatagcaaa tgcgatatac catggcaagg taaaaatcag aatgccgtaa
    13501 aaggcgatat aggatgccac catcagcaaa tccgcatgcg caaaattgat cagccgcaag
    13561 atcccgtata ccatggtgta gcctatggcg atcagcgcat aaagactgcc cagggtaatg
    13621 ctgttggcga tattttgcag gaatatgtcc atggacaagc gttatcagct cctttaaatg
    13681 cagtcagaac gagattaatg cgagctttgg gcggcggaca aaatagatgc tcccctcctg
    13741 ttgggcagga gaggagcaga tttatcgtac caaccttact tgggatcgac cttgccttca
    13801 aatacgaact ttccgtccgc gactttcttg atgatggcgg tcttggtggc atcgcggttt
    13861 tcatccaggg tgataatgcc ggtggcgccc tggaagtctt tggtctgagc cagggcatct
    13921 ctgatcttga cagaatcagc ggtatcggct ctggtgatgg ca
//
LOCUS       DAOTHZ010000056        13697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5367_length_13697_cov_8.100279, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000056 DAOTHZ010000000
VERSION     DAOTHZ010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 13697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13697
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_13697_cov_8.100279"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..2384
                     /locus_tag="P5549_07410"
     CDS             441..2384
                     /locus_tag="P5549_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRW12937.1"
                     /translation="MIILQAQKINKSFADQHVLRDISLTLQVRERVGMVGVNGAGKTT
                     LLQCLAGLLLPDSGEISYSADMSMGYLQQLADYEDGITLWDAIMESYAEVLQLRSRLT
                     ALEEQIARGGPDLNKDLQRYARLSDEYERADGYACENTARRILSGLGFPPHEYEQQAD
                     RLSGGQKTRFNLACLLAKSPDLLFLDEPTNHLDIASVEWLEEFVKNYPGTVLVVSHDR
                     RFLDKIATRILDLRGGKISSYEGNYSQYLVKRAADDLAWQRAYEKQQEHIRETEAYIL
                     RFKAGIKSKQARGRQSQLQRLERIDRPQQEKNLSLRKFNINQASGRDVLRLKSVAKSF
                     DTNQLFEKLDLNIFKGEKIALVGPNGCGKTTLLKIISGRLPADAGEVRLGSRVEIGYY
                     GQEYEELDSNHTLLEEILENFDMEIEPARTALGSMLFSEDEVFKQVKNLSGGEKGRLG
                     LLKLILSGANFLILDEPTNHLDLDSCAIVEKMLQDYEGTVLLVSHDRYFIDQVAERII
                     TFNNGALESYPGNYSYYQEKLLAQNKADTEARRQAARERNQPAIEAREHRKEQQRIRR
                     QRQRALEEIELRIAELEEQKSRLEIELSDPETYRDEENARKVNEAYKGLEEALLTAYW
                     KWEEVSAMLEEEMKGDNDELFAR"
     gene            2365..4290
                     /locus_tag="P5549_07415"
     CDS             2365..4290
                     /locus_tag="P5549_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRW12938.1"
                     /translation="MNYLPVNRQDLTKRGWDELDIIFVSGDAYVDHPAWAAALLGRFL
                     EKQGFRVGIIAQPDWQSLEDIRKLGQPRLFFAVSAGNMDSMVSHYTADKKRRSEDLYS
                     PGGQAGLRPDRTSIVYTNLIRQAWPSVPVVIGGVEPSLRRLAHYDYWSDKVRRSLLAD
                     SKADLLVYGMGEYPLLEIARQLKSGKEIRYLTNLRSTVYMSKSMPEEALALPAYEKVK
                     QDQVAFAAATRLIHQELNPYCARPLAQQHGERWVVVNPPALPLSTAQMDEIYGLEFTR
                     RAHPVYEEQGGIPALEPVQFSVTTHRGCFGGCAFCSLGLHQGKFIQNRSKESIIHEVE
                     EISRHPDFKGTIPDVGAASANMFGLHGKDEQRCQSCRRESCLYPTICRNLQTDHSPSV
                     KLWKELRRNPRIKHMFVASGVRYDLILKDSTGQYLKDLCKYHVGGQLKIAPEHVSDRV
                     LKLMRKPSQAEYKKFIEKFQKENQQLGKEQYLVPYFISAHPGSQLHDTIELAEFVRDN
                     LQYYPEQVQNFTPTPMTVSTCMYHTGLDPVSQQSVYIPRQIRERKWQRALLQYRDSRN
                     RSLVREALQSCGREDLLNSSPQGLLARAAKPAVKPNERPKRLATGKKNQTAKESGNAS
                     RKNRKSHSGRRRSGAQS"
     gene            4229..5317
                     /gene="hflX"
                     /locus_tag="P5549_07420"
     CDS             4229..5317
                     /gene="hflX"
                     /locus_tag="P5549_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRW12939.1"
                     /translation="MQAEKIEKAILVGADLGRKAEVSFLDSLEELKGLTEAAGGEVIA
                     TLVQARERIHNTTYIGSGKLQELKHLASELGPDLIIFDQELSPVQLRNLEEELDCKVI
                     DRTMLILDIFSQRARSNEGKLQVELALLQYRLPRLTGKGAAMSRLGAGIGTRGAGEQK
                     LELDRRYIRRRIQEVKKKLEKAKSTRNLQRKQRQRTGMKIVSLVGYTNAGKSTLFNTI
                     CQSSHRSGAAQVEADDRLFQTLDTTIRKVYLPSRREILLTDTVGFIQQLPHHLMAAFR
                     STLEEAVDADLLLHVVDMADPQVLDKMVVVEEVLKDLGADPERVLTVFNKSDRLDDPP
                     LPGGLAVSALTGQGIGALLDEIEKLFTQ"
     gene            complement(5322..5729)
                     /locus_tag="P5549_07425"
     CDS             complement(5322..5729)
                     /locus_tag="P5549_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRW12940.1"
                     /translation="MAGSDEVKRKVLNDCKTVAIVGLSDNPQRASHRIGKYLKENGYK
                     IVPVNPALSEVLGEKAYPDMASIPFAVDLVDVFRRQEEVDAVVEEALRINPKAVWLQL
                     GLTCPGDKGRVREAGADLVEDCCVMVEHRRLIG"
     gene            5908..7134
                     /locus_tag="P5549_07430"
     CDS             5908..7134
                     /locus_tag="P5549_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRW12941.1"
                     /translation="MSWSNNPKRTYLVLAAVFLLTFAIGSWAGGPIKKMFSDSIFELS
                     AKGKPINMLFMGIDDRENKSNTRSDTMIVVSIDPKEKKMVMVSIPRDTRVKDALGRSD
                     RINSINYTKGPEAACKVVSDLMGVPVNYYVITNFAGFGKIVDILGGVDLDVETDMYHW
                     DVSYIIDLKKGYQHLNGDQALQYVRYRGGPTADIGRTQRQQKFIKALAKQMFQAKTVL
                     RLPELVPEISKNVKTNIPVSDMPYLAKLAQSIDTSTINGQTLPGYPYTDPGNGASYWE
                     PDRAKAKTILAALREGKSFDVVGDPPSWINKSPEKYEPQELPSADILQEQDELSTIDP
                     EAATANEPGQGDRNPEGNGKVGEGEELVTTGNNIEEPETDKGEKNPGIEELPADTTDT
                     PSPADQSPTGEPSEQN"
     gene            complement(7207..7713)
                     /locus_tag="P5549_07435"
     CDS             complement(7207..7713)
                     /locus_tag="P5549_07435"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRW12942.1"
                     /translation="MSIDIPYDQSAAQFLTQLPRGAFLTVKAGDQLNTMTIGWGSIGH
                     IWQKPIMMVMVRYSRYTYELMEKAVDFTVSIPATGELKKDLAVAGTKSGRDIDKFQES
                     QLTAKKAHLIDSPIISECGLAYECKIIYKQEMQPENMADEYKNRCYANGDFHVMYFGE
                     IVACYKPE"
     gene            7955..8039
                     /locus_tag="P5549_07440"
     tRNA            7955..8039
                     /locus_tag="P5549_07440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7989..7991,aa:Leu,seq:tag)
     gene            8487..10583
                     /locus_tag="P5549_07445"
     CDS             8487..10583
                     /locus_tag="P5549_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRW12943.1"
                     /translation="MSATILVVDDSASDRLIIKNMLNEYRVLTACDGLEAMREIDAQA
                     DIDLVILDLNVPNMNGFEVLGALQSNERYKKLRTLILTNHDELDNEIKGLKMGAVDYI
                     RKPIHMEFLKARMEIHLELLRIQKLVEHKLYEQGLTLDTVFSQAPIGIAINEPSDDAG
                     NGMVAINPMYEQITGRSKEELLKLGWGRITHPDDLDEDLQNLQKLQAGEIKSYIMEKR
                     FIKPDGSIVWVHMVVAPLTLSNQSKFNHICLVQDISERKRMENDLRYNNEHDLWTGLY
                     NRSYLETLLKKEARLQDAKKSALVGINLGAVHLLSITYGFQYSQELIKKAAVALQSHC
                     HENCALFKMYENHFAFYIKAYQEKNELTAFCQALGGTLEALLIVERIGAGIGIIEIDD
                     NNKHDVGVLLKNLLIASEKAINVFDSDIGCCFFDEEMEKQIIRAEEIKRELAQIAADG
                     DSDRLFLQFQPILDLQTNRICGFEALARVIIDKLGLIMPLEFIPMAEETKLINPLGER
                     IILQALHFLQTLQAKGYHDVCVSINISAIQLLRNDFIQKLMEMIDEIEVHPGNIGLEI
                     TESVFSSNYQELNRTLGELKDLGIQVAIDDFGTGYSSLARERELNVSCLKIDKFFIDK
                     LLMLKDEQTITGDIIAMAHKLGQCVIAEGIEDEKQLQYLKRYGCDRIQGFLISEPLDE
                     AAALELLQQQTGIMKG"
     gene            10676..11599
                     /locus_tag="P5549_07450"
     CDS             10676..11599
                     /locus_tag="P5549_07450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW12944.1"
                     /translation="MSVIFADINGLKMTNDIFGHAAGDKLIIKSAEILRQSCRENDII
                     ARMGGDEFIMLLPQTNEEEAREILARISSGFLNARIEAIKCSISLGLDTKSSAEQSLE
                     VTMTNAENAMYKDKTLNRQSINRDIISAIVETLHTRSPREKQHSLTVSILCGEAGSAL
                     NLSEAEINQLKRAGYLHDIGKITLDESILLKDSLTAEEYEKMQQHSVVGYRILNLFDD
                     TLDLAEYVYSHHERWDGSGYPRGLQGEQIPKLSRILAVVEAYERVLNRGNLSLAERKQ
                     NALGIIKEGAGTQFDPQIAAFFIQMMNEHSG"
     gene            11652..11915
                     /locus_tag="P5549_07455"
     CDS             11652..11915
                     /locus_tag="P5549_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12945.1"
                     /translation="MCFFKQEVYMGYSLADLAKVRQALVNEGIKYSYRVVDHSGQWAG
                     RGTIRGNYGSMGMNMDYARQYSVAVKRKDFEKASYVVQRALHS"
     gene            11942..12661
                     /locus_tag="P5549_07460"
     CDS             11942..12661
                     /locus_tag="P5549_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW12946.1"
                     /translation="MFLKSVSLLREKIASFDNYPFSIPAIKSLEQIEFKSRVTFFVGE
                     NGSGKSTLLEAIAAQCGFNTAGGGRNNLYEVHAAKSDLADHIRLAWMPKVTNGFFLRA
                     ESFYHFASHLDEVDDTGFRAYGGRSLHQQSHGESFLSLFMNRFNGKALYLLDEPEAAL
                     SPARQLAFLRILHDLTDSGDAQFIIATHSPILMGYPDAVILSFDDSQIKDVAYEMTDH
                     YLITKYFMQHREKVLADLLAE"
     gene            complement(12771..13460)
                     /locus_tag="P5549_07465"
     CDS             complement(12771..13460)
                     /locus_tag="P5549_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRW12947.1"
                     /translation="MENNRDSSYRLDWQTLRRDWPLWVIMTGLVVTAILVYPHLPDQV
                     PRHWDIHGNVDAYSSRLFGAFFPPLMSIGLYFLLLFTPLLDPKRDNYSRFAQGYTAIR
                     WGVVLLLSILYVASILFALGYPINISFIVKGLVAGLFIMIGNYMGQFRHNYFVGIKTP
                     WTLASEEVWQRTHRMASRIWVAFGLLCLVMSFIDAVWSGVVYFVAILLMVIIPSVYSF
                     VIFNGMPKSNQ"
     gene            complement(13444..>13697)
                     /locus_tag="P5549_07470"
     CDS             complement(13444..>13697)
                     /locus_tag="P5549_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRW12948.1"
                     /translation="AMTKPSISHHLNILKQANLVQDIRKGQNIYYSLNTTVFQEVIGW
                     LYDFLTPDEKEKSEEKSANGSNDHSQPFNKGGNNNGKQS"
BASE COUNT         3884 a         3064 c         3452 g         3297 t
ORIGIN      
        1 ttgtcttctt tgagacgact ttttttcagg ttggtttccc gaaaggactc atggtcaatg
       61 agtcggtcca atacactggg gtcggaaata cgtagatttt ctagagcctc ctcattatag
      121 gaaaactttg ctgccaaggt cacccctcct aaaaataata tttaaaatat tgataattaa
      181 tattataacg taataattgc atttcgcata taataaatat ttctaataag gaaattgcat
      241 aaaataagga cattttggca gtattgcagc agaattggtt ggaaaaagaa tgtgaaatgt
      301 gaaaaaattg cttctggaat tggatacgat aatgcgtcag agatcaaaaa ggattcgctg
      361 caataaatat aagcagaaga acatgacgtg atataattaa acattgtatg aaccggctgt
      421 taaatttgcg tggtggaaaa atgatcatat tacaagcaca aaaaattaat aaatcgtttg
      481 ctgatcagca tgttctgcgc gatatcagtc taacgcttca agtccgggaa cgggttggaa
      541 tggtgggggt caacggagcc ggcaaaacca ctttgctgca gtgtctggcg ggtttgctgc
      601 tgccggacag cggcgagatc agttattcag cagatatgtc catgggttat ctgcagcaat
      661 tggcggatta tgaagacgga ataaccctct gggatgccat catggagagt tatgctgaag
      721 ttttgcagct cagaagcagg ctgacggcgt tggaggagca gattgcccgg ggcggtccgg
      781 atcttaacaa agatcttcag cgctatgccc gtttgagtga cgaatatgaa agggcagacg
      841 gttatgcctg tgagaatact gcccggcgaa tcctcagcgg actgggtttc ccgccgcacg
      901 aatatgagca acaggcggat cgtctgagcg gcggacaaaa aactcgtttt aatctggcct
      961 gcctactggc taaatcaccc gatttgctgt ttctggatga accgaccaat catttggata
     1021 ttgcctccgt cgaatggctg gaggaattcg tgaaaaacta tccgggaacg gttctggtgg
     1081 tctcccatga ccgcaggttt ttggataaaa ttgcgacccg catcctggac ttgcgcggag
     1141 ggaaaatttc atcgtatgag ggcaattaca gtcaatatct ggtcaagcgt gccgccgatg
     1201 acttggcctg gcagcgggct tatgaaaaac aacaggaaca cattcgtgag acggaagcct
     1261 atatccttcg cttcaaggcg ggcatcaaat ccaaacaagc ccggggccgg cagtcgcaac
     1321 tgcagcgtct ggagcggatt gaccgtcccc agcaggaaaa aaacctgagt ttacgaaagt
     1381 tcaatataaa tcaggccagc ggacgtgatg tgctgcgact aaaaagcgtc gccaaatcat
     1441 tcgataccaa ccaacttttc gaaaaactgg atctgaatat tttcaaaggg gaaaaaattg
     1501 ccctggtcgg acccaacggc tgtggcaaaa ccactttatt aaagatcatc agcggtcgtt
     1561 tgccggctga tgcaggggag gttcgactgg gcagccgggt agagatcggt tattacgggc
     1621 aggaatatga agaattggac tctaaccata cgctgctgga agagatcctg gagaatttcg
     1681 acatggagat cgagccagcc cgaaccgctc tgggatcaat gctgttcagc gaagatgagg
     1741 tgttcaaaca ggtcaagaac ctgagcgggg gagaaaaagg tcgtttgggg ctgctcaaac
     1801 tgattctttc cggggccaat tttttaatcc tggacgaacc taccaaccat ctcgacctcg
     1861 acagttgtgc cattgtggaa aagatgctgc aggattatga gggtacggtt ctattggtat
     1921 ctcatgatcg ctatttcatt gatcaggtgg ccgagcggat aattaccttc aacaacggtg
     1981 cacttgagtc ctatcctggc aactacagtt attatcagga aaaattgctg gcacaaaaca
     2041 aagcggatac cgaagcccgc cggcaagctg ccagggaacg caaccaacca gccatcgaag
     2101 cccgggaaca taggaaagag caacagagaa ttcggcgcca aagacagcgc gctctggaag
     2161 agattgagct gcgaatcgcc gaactggaag aacaaaaaag ccggttggaa atcgaattgt
     2221 ctgatccaga gacgtatcgt gacgaggaaa acgcccgtaa ggtcaatgag gcatataaag
     2281 ggctggaaga agcgctgctt accgcttact ggaaatggga agaagtcagc gccatgctgg
     2341 aagaagaaat gaagggtgac aatgatgaat tatttgcccg ttaaccggca ggatctcacc
     2401 aagcgtggct gggatgaatt ggatattatc tttgtctctg gcgacgccta tgtcgatcat
     2461 cccgcctggg cagcggcgtt gctgggccga tttctggaaa agcagggatt cagggtaggg
     2521 attattgccc agcctgattg gcaaagtctc gaggatatcc gcaagctagg ccaaccccgt
     2581 ttgttttttg cagtgtctgc cggcaatatg gacagtatgg tcagccatta tacggcggat
     2641 aaaaaaaggc gttcggaaga tttgtactca cccggcggac aggctgggtt gcgtccagat
     2701 agaaccagca tcgtttatac caacctgatc cgccaggcct ggccgagcgt gccggtcgtg
     2761 atcggcgggg tcgaacccag tttgcgacgt ttggcccatt atgattattg gagcgacaaa
     2821 gtacggcgtt ccctgctggc cgacagcaag gccgacctgc tggtctatgg catgggcgaa
     2881 tatccgctgc tggagatcgc ccgccagctc aaatccggca aggaaatccg ttatctgacc
     2941 aacctgcgca gtactgttta tatgagcaaa tccatgccgg aagaggcgct ggcgctgcct
     3001 gcctacgaga aagtcaagca agatcaggtc gcttttgccg ccgcaacccg gctgattcat
     3061 caggaattaa atccttattg cgcgcgccct ttggcgcagc agcacgggga acgctgggtg
     3121 gtagtcaatc ctccggcatt gccgctcagc accgcccaga tggatgaaat ttatgggttg
     3181 gaatttaccc gccgagctca tccggtatac gaagagcagg gagggatccc ggcgctggag
     3241 ccggtacagt tctccgtcac tacccatcgg ggttgtttcg gcggttgtgc gttttgttcg
     3301 ctgggcttgc atcagggtaa attcattcaa aaccgcagta aagaatcgat catccatgag
     3361 gtcgaggaga tcagccgtca ccctgacttt aaaggcacca ttccggatgt aggagccgct
     3421 tctgccaaca tgtttggctt acatggaaaa gatgaacagc gctgtcaaag ctgccgtagg
     3481 gaaagttgcc tctacccgac gatttgcagg aacctgcaga ccgatcacag tccttcggtc
     3541 aagctttgga aggaattgcg ccgcaatccg cgcatcaagc atatgtttgt ggcctcgggt
     3601 gtccgctatg atttaatcct gaaagacagt accggtcagt atttaaaaga cctttgtaaa
     3661 tatcacgtcg gcgggcagtt gaagatcgcc ccggaacatg tgtccgaccg ggtgctgaaa
     3721 ctaatgcgca agccttcgca ggcggaatat aaaaagttta tcgagaagtt tcaaaaagaa
     3781 aaccaacaac tgggcaagga acagtatctg gtaccttatt tcatttccgc ccatcccggc
     3841 agtcaactcc atgatacgat tgaactggcc gaatttgtcc gcgataattt gcagtattac
     3901 ccggaacagg tgcaaaattt cacccctacg cccatgacgg tttccacctg tatgtatcat
     3961 accggactgg acccggtcag ccaacaaagc gtatacattc cccggcagat acgagagcgc
     4021 aaatggcagc gagcgctatt gcaataccgc gattcccgca accgcagtct ggtcagggaa
     4081 gccctgcaaa gctgcggtcg ggaagacttg ctcaactctt cgccacaagg attgctggcc
     4141 cgggcagcga aacctgcagt caaaccaaat gagcgcccca aaaggctggc tacagggaaa
     4201 aaaaaccaaa cagcaaagga gtcaggcaat gcaagcagaa aaaatcgaaa aagccattct
     4261 ggtcggcgcc gatctggggc gcaaagctga ggtcagtttt ctggattctt tggaagagct
     4321 aaaaggcttg accgaagctg ccgggggaga agtaatcgcc actctggtac aggcccggga
     4381 gcggatccac aataccactt atattggcag cggcaaatta caggagttga aacatttggc
     4441 gtctgaactg ggacccgatc tgattatttt tgatcaagaa ctgtcgccgg tgcaactgcg
     4501 caatctggaa gaagaactgg actgcaaggt gatcgatcgt accatgctga tcctggacat
     4561 tttcagccag cgagctcgtt ccaacgaagg caagctgcag gtcgaactgg ctctcctgca
     4621 gtatcgtttg ccgcgcctga ccggcaaggg ggcagccatg agccgcctcg gtgcaggaat
     4681 tggcacccgc ggtgccgggg aacaaaaact ggaactggat cggcgctata tccgccgacg
     4741 tattcaggag gtcaaaaaga aactggaaaa agccaaaagc actcgcaact tgcagcgcaa
     4801 acagcgtcag cgcacaggca tgaagatcgt atccctggta ggctacacca acgcgggcaa
     4861 gtctacgttg tttaatacca tttgccagtc gtcgcatcgc agcggagctg cgcaggtgga
     4921 agctgacgac agattgtttc aaaccttgga tactacgatt cgcaaagtct atctgccgtc
     4981 ccgccgggaa attttgctga ctgatacggt ggggtttata cagcaactgc cgcatcactt
     5041 gatggctgcc ttccgttcca cgctggaaga ggcggtggat gcagatctgc tgttgcatgt
     5101 cgtggatatg gccgatcctc aggttcttga caaaatggtc gtagttgaag aggttctgaa
     5161 agacctggga gcagatccgg aacgggttct gacggtcttt aacaaatccg atcggttgga
     5221 tgatcccccc ctgcccggcg gtctggctgt ttctgccctg accggacagg ggattggggc
     5281 cttgttggat gaaatagaaa agctgtttac gcaatgatct gctaacctat gaggcgacgg
     5341 tgttcaacca tcacacagca atcctctacc aaatcagcac cagcctccct gacccggcct
     5401 ttgtctcccg ggcaggtcag gcccaactgc agccaaaccg ccttggggtt gattcttaac
     5461 gcttcctcaa ccacggcatc aacttcttcc tgccggcgaa aaacgtccac caaatcaacc
     5521 gcaaaaggaa tggaagccat atccggatag gctttctcgc ccaacacttc agacagagcc
     5581 gggttaaccg gcacgatctt atagccgttt tcttttaaat acttgccaat gcgatgactg
     5641 gcccgctgtg gattgtccga caggccaacg attgctactg ttttgcagtc gttcaatact
     5701 ttgcgtttta cttcatctga tcctgccatg ggtataacct ccttagaata tgatggtaca
     5761 tcaacctgag attactccta atcatatcct ctttttgccc gggaatgaag tgaaaaatac
     5821 atggacggct gaggagcctg ggagtagaat aaatatgatc attaataacc gattgaaaat
     5881 attttcatat aagagaggtt ttttccaatg tcatggagca acaatccaaa aagaacatac
     5941 ctcgtattgg cagcagtatt tctgctgact tttgccatcg gcagttgggc aggaggccca
     6001 atcaaaaaga tgttttccga ttcaatattt gaactgagtg caaaaggcaa acccatcaat
     6061 atgttgttca tgggcatcga tgatcgagaa aacaaaagca atacccgcag cgataccatg
     6121 attgtggtca gcattgatcc caaggaaaag aaaatggtca tggtctccat cccgcgcgat
     6181 acccgggtta aggatgcgct gggacgcagt gacagaatca acagcatcaa ctacaccaag
     6241 gggcccgagg ctgcctgcaa ggtcgtcagt gatctgatgg gggttccggt caattattat
     6301 gtcattacaa atttcgccgg gtttggtaaa atcgttgaca tcctgggcgg ggtcgacctg
     6361 gatgtggaaa ccgatatgta ccattgggac gtcagttata ttatcgacct gaaaaagggt
     6421 taccagcatc tcaatggtga tcaggcgctg caatatgtgc gctaccgggg cggtccgacc
     6481 gctgacatcg gccggaccca gaggcaacaa aaatttatca aagccctggc caagcaaatg
     6541 tttcaagcca agaccgtgct gaggctgccg gaactggtgc ccgagatttc taaaaacgtt
     6601 aaaaccaata ttcctgtcag cgatatgccc tatctggcta aactagctca aagcatcgac
     6661 acctcgacga tcaatggcca gaccttgccc ggttatccct acaccgatcc gggcaacgga
     6721 gcaagttact gggaacctga ccgggccaag gccaaaacaa ttcttgctgc cttgagggaa
     6781 ggcaagagtt ttgatgtagt gggcgatcct ccctcctgga tcaacaagtc tcctgagaaa
     6841 tatgaacccc aggaactgcc cagtgcagat attttgcagg aacaagatga attgtcgaca
     6901 attgatccag aagcagcaac tgcaaacgag cctggccaag gagatcgtaa tcccgaaggc
     6961 aatgggaaag tcggtgaagg agaagaactg gtaactacag ggaataatat tgaagaaccg
     7021 gaaacagata aaggtgagaa aaatcctggg atagaagaac tgccggctga tacaacggat
     7081 acgccctccc cggcagatca aagcccaacc ggcgaaccat cagagcagaa ttaacaaccg
     7141 atttaaaaaa agattccata ccaacgcagg ttgataagga atcttttttg tatttgggtt
     7201 aaaattttat tccggcttgt agcaggctac aatttccccg aaatacatca catgaaaatc
     7261 accattggca tagcatctgt ttttatattc atcggccata ttttccggct gcatttcctg
     7321 cttgtaaata atcttgcatt cataggccag accacattcg ctgatgattg gactgtcaat
     7381 caaatgggcc tttttggcgg tcagctggct ttcctggaat ttatcaatgt cccgtccgga
     7441 cttggttccg gccacggcca aatccttttt aagttcacct gtcgcaggga tactgaccgt
     7501 aaaatcaact gctttttcca tcagttcata agtataacgg gaatagcgca ccatcaccat
     7561 cataatcggc ttttgccaga tgtgaccgat tgagccccag ccgatcgtca tggtattgag
     7621 ttggtcaccc gccttgacgg ttaaaaaagc acctcggggc agttgggtca gaaactgggc
     7681 ggcagattga tcataaggaa tatcaataga cataaagaaa tctcctttcg aatcgctggt
     7741 tgatttacaa ccaatatatg ctaaatttcc gtgagaggaa cttataaaaa gtatataaca
     7801 aaagcaatgt ttttatcatt atttcataag caagccgtta tagaatggca gggattgtcc
     7861 tacattgacg aatttacttt gcccctctat aatgatagag tgcgttggtt ggcattaaaa
     7921 aacactcttt gcgaatcaac ctcatataca attggcggat gtggcggaat tggcagacgc
     7981 accagactta ggatctggcg tttcacgacg tgggggttcg agtcccttca tccgcaccat
     8041 tgaatttacg ggaggcttag gcctcccttt ggtgcttctg ggagggaatt atttattttg
     8101 gaaggatttt tccccggaat tttcattgcc agacgaagtg gatataaata tgcctgccgg
     8161 atggatggct gtgggtattg aataattggg aaacagacaa gaatattgct ggaaatagtt
     8221 tgaaatttct gtaatttgtc gtatacttga accatgctaa caaatgttta aagagcattt
     8281 ggatttgcag taattttatg aaaatgcagc agctttgcca gtctcatgag ttataaccaa
     8341 tgaaaaacca aatcgaacag cctttcgttt tttaaaacat ggttacgatg tgaccagcat
     8401 cagaatgatc tgccaggaag cctgactaga cccgttacca atgccacggt acaaggagag
     8461 ggtattacaa tgagggagga tgagttatgt ctgcaacaat attggttgtt gacgattcag
     8521 cgtcagaccg gttaatcatt aagaatatgc tgaacgaata tcgggttcta actgcttgcg
     8581 atgggttgga agcgatgcgc gagattgatg cgcaggcaga tattgatctg gtgattctcg
     8641 atttgaacgt gcccaacatg aatgggtttg aggttcttgg cgccctgcaa tccaatgaac
     8701 gatataaaaa actgcgtacg ctcatattaa ctaaccatga tgaactcgat aatgaaatca
     8761 aagggctgaa aatgggggcg gttgactata tcagaaagcc aattcatatg gagttcctga
     8821 aggccagaat ggagatccat cttgaattac tcagaatcca gaaattggtt gaacacaagc
     8881 tgtatgaaca aggtttaacc ttggataccg tttttagcca agccccgatt ggaatcgcca
     8941 ttaatgaacc atccgatgat gccgggaatg gcatggttgc catcaacccg atgtatgagc
     9001 aaatcaccgg cagaagcaaa gaagagctgc tgaagctggg atggggaagg attactcacc
     9061 cggatgactt ggatgaagat ctgcaaaacc tgcagaagct tcaagccggg gaaattaaaa
     9121 gctatatcat ggagaagcgg tttatcaagc cggacggttc gatcgtttgg gtacatatgg
     9181 tggtagcgcc gctgacgctg tcgaatcaaa gcaaattcaa tcacatctgt ctggtgcagg
     9241 atatctcaga gcgaaaaaga atggaaaatg acttgagata caataatgaa catgatctct
     9301 ggacgggact gtataaccgc agttatcttg aaacactgct aaaaaaagaa gccaggttgc
     9361 aggatgccaa gaaaagtgca ttggtaggca ttaacctggg cgcggtacat ttattgagca
     9421 ttacgtacgg atttcagtac agccaggaac tgattaaaaa agctgctgtt gcattacaat
     9481 cacattgcca tgaaaattgc gcattgttca aaatgtacga aaatcatttt gctttctata
     9541 tcaaagccta tcaagaaaag aatgaattga ctgcgttttg ccaagcgctt ggaggtaccc
     9601 tggaagcatt gctgatagtc gaaagaattg gtgcggggat tgggattatt gaaatcgacg
     9661 ataataacaa gcatgatgta ggggttcttt taaaaaatct actcatcgcc tctgaaaaag
     9721 ccataaatgt atttgacagc gatataggtt gctgtttttt tgacgaagag atggaaaagc
     9781 aaatcattcg tgcagaagag atcaaacgcg aactggccca aatcgcagca gatggagaca
     9841 gtgacagatt gttcctgcaa tttcagccaa ttttggatct tcagacaaat cggatttgtg
     9901 gttttgaggc cttggccaga gtaataattg acaagctggg gctgatcatg cctttggagt
     9961 ttatcccgat ggctgaggag acgaaactta ttaatccttt gggggagaga attattctgc
    10021 aggcattgca tttcttacag acattgcagg ccaaaggtta tcacgacgtc tgcgtatcca
    10081 taaatatttc tgccattcag ttgctgagaa acgattttat ccaaaaactc atggagatga
    10141 ttgatgaaat tgaggttcat cccgggaata tcggtctgga aatcaccgaa tcagtctttt
    10201 cttccaacta ccaggaactc aaccggactc ttggcgaatt aaaggattta ggcatccagg
    10261 ttgcgatcga cgactttggt acgggatatt cttctttggc gcgggaacgg gaattgaacg
    10321 tcagctgcct gaaaatcgat aagtttttta ttgacaaact gttaatgctt aaagatgagc
    10381 agacgatcac tggtgatatc attgccatgg cccacaaact gggacaatgt gtcatcgccg
    10441 aaggaatcga agatgaaaaa caattgcaat atctcaagcg ctacggatgt gacagaattc
    10501 aagggttcct catcagcgaa ccactggatg aggcagcagc cctggaactg ctccagcaac
    10561 agaccggtat aatgaaagga tgaaggtttt tcgaatgaca acctaagttg aaaaaggatg
    10621 gtgcaggtga aggagaacgg aaaacatcct ggttgatatt cctgataacc tgtcgctgtc
    10681 ggttattttt gcggacatta acggattgaa gatgacaaat gatattttcg gccatgctgc
    10741 cggagacaag ctgatcataa aatcagccga aatcctccgc caatcctgtc gggaaaatga
    10801 tataattgcg cgcatgggcg gcgatgagtt cattatgctt ttgccccaga cgaatgaaga
    10861 agaggccagg gagattctcg cccggataag ctccggtttc cttaatgcac gaatcgaagc
    10921 gataaagtgc agcatatcgt taggtttgga tacgaaaagc agtgcggagc agtcactcga
    10981 ggtgaccatg accaacgcgg aaaatgcgat gtacaaagac aaaactttga accgtcaatc
    11041 gattaaccgg gatattatca gtgccatcgt tgagacgctg cacacccgca gtccccgcga
    11101 gaaacaacat tctctgaccg tcagtatatt atgcggtgaa gcgggcagtg ctttaaatct
    11161 atcggaagcg gaaatcaatc agttaaagcg ggcaggttac ttgcatgaca ttggcaaaat
    11221 taccttggat gaaagcattc tgttaaaaga ttccctgacc gcggaagaat atgaaaaaat
    11281 gcaacagcat tctgtggttg gatatcgtat cttgaatctt tttgatgata ccctggattt
    11341 ggcggaatac gtctacagcc atcacgaaag gtgggacggc agcggctatc cgcgcggact
    11401 tcaaggggaa cagattccaa agttatcaag aattctggcc gtcgtggaag cctatgaacg
    11461 ggtattgaac agagggaatc tttccttggc tgaaagaaaa cagaatgcct tgggtattat
    11521 taaagagggg gcaggaacac aatttgatcc gcaaattgct gcgtttttta tacagatgat
    11581 gaatgagcat agcggatgaa gcgcgaaagg ataatgtaaa ggcaatcccg gcggataagg
    11641 aggcgagtac catgtgcttt ttcaagcaag aggtatatat gggctactca ttggcggacc
    11701 tggccaaagt cagacaggcg ctggtcaatg aaggcattaa atactcgtac cgggtggtcg
    11761 atcattcagg ccaatgggcg ggtcggggaa ccatccgggg caactatggg agtatgggca
    11821 tgaacatgga ttatgcccgg caatattcgg ttgcggtaaa aagaaaagat ttcgagaaag
    11881 cgagttatgt tgtgcagcga gccttgcatt cataagcatg tttaggagag atgagatact
    11941 tatgttttta aaatcggtca gcttgctcag ggaaaagatt gcgtcttttg acaattaccc
    12001 tttttccatc ccggcgatca agagtctcga gcaaattgaa ttcaagagcc gggtcacctt
    12061 ttttgtgggc gaaaacggat caggaaaatc tacattatta gaagcaatcg cggcgcagtg
    12121 tggttttaat acggccgggg gcgggagaaa caacctgtat gaagtgcatg ccgccaagtc
    12181 tgatttagcc gatcacatcc gactggcctg gatgcctaag gtcaccaacg gattttttct
    12241 gcgggcggaa tctttttatc attttgcttc tcatcttgat gaagtggatg ataccgggtt
    12301 cagagcttac ggcggacgtt ctctgcacca gcaatctcat ggcgaatcat ttttatcctt
    12361 gtttatgaac cgcttcaatg gcaaggcgct ctatttattg gatgaaccgg aagccgcctt
    12421 gtcaccggcc cgacaactgg cctttctgag gatattacat gatctgaccg acagcggtga
    12481 tgcccagttt atcattgcca cccattctcc catcttaatg ggttaccctg acgcagttat
    12541 tttgagtttt gatgacagcc agatcaagga cgtagcctat gaaatgaccg atcattatct
    12601 catcaccaag tattttatgc agcaccggga aaaggttctg gcagacttgt tggcagaata
    12661 atccgatctc attccggcaa cacctcacag cccggacact catcaatacc caccatccgc
    12721 attaagaaaa ggctgacaac accatcccca gcaaaacata ttcgaggaaa tcattgattg
    12781 ctttttggca tcccgttgaa gatcacaaag gagtatacgc tagggatgat gaccatcagc
    12841 aaaatcgcca cgaagtacac cacgccgctc cagacagcat ctataaaaga catgaccaga
    12901 cacagcaatc caaatgccac ccagatgcgg ctggccatac ggtgcgtccg ttgccatact
    12961 tcttcgctgg ccaatgtcca gggagtcttg atgccaacaa aatagttgtg cctgaattgg
    13021 cccatataat tccctatcat aataaacaaa ccagccacca aaccttttac aataaaactg
    13081 atgttgatag ggtaacccag cgcaaacagg atgctcgcta catatagaat gctgagcagc
    13141 aggaccacgc cccagcgaat ggcagtatag ccctgggcaa agcgagaata gttgtcccgc
    13201 ttgggatcca gcaaaggagt gaacaataaa agaaaataaa gaccgatact catcaacggc
    13261 ggaaaaaagg cgccaaacaa gcgggatgag taagcatcta cattgccatg aatatcccag
    13321 tgacgcggaa cttggtcagg caaatgcgga taaaccaaaa tcgccgttac aaccagcccg
    13381 gtcataatca cccagagcgg ccaatcccgg cgcagcgttt gccagtccaa acgataggaa
    13441 gaatcacgat tgttttccat tattattgcc tcctttatta aaaggttggg agtggtcgtt
    13501 tgaaccattg gcactttttt cttcgctttt ctccttttca tcaggggtta agaaatcata
    13561 caaccagccg attacttcct gaaaaaccgt tgtatttagt gagtaataaa tattttgtcc
    13621 tttgcgtata tcctgcacca gattggcctg tttcaggata ttgagatgat gcgatatact
    13681 gggtttggtc atggcaa
//
LOCUS       DAOTHZ010000057        13154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5647_length_13154_cov_7.956638, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000057 DAOTHZ010000000
VERSION     DAOTHZ010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 13154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13154
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5647_length_13154_cov_7.956638"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="P5549_07475"
     CDS             complement(<1..626)
                     /locus_tag="P5549_07475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRW12949.1"
                     /translation="MQKIILASASPRRQQLFAQIGLPFVAAAADVDEEPMKECSAPEL
                     VQQLARRKAEQVAGVWRDAIIIAADTLVILDEHIMGKPVNREDAFRKLSLLSGRHHKV
                     MTGLCVLDSNRGICDTGVESTEVYFRTLTPGEINAYLNWGEWVDKAGGYGIQGKGALL
                     IEKIEGCYFNVVGLPINRLYIMLGKQGVNLLGGKTPDGLPVWDQGHAPG"
     gene            complement(650..916)
                     /locus_tag="P5549_07480"
     CDS             complement(650..916)
                     /locus_tag="P5549_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HRW12950.1"
                     /translation="MANRTRTYPGWQFLLLLLALGGIIGGWIGDAIVRLWPALALLGR
                     IQSVGLPTFTMDLHVFTLSFGFMLNINFFTILGFVLAWLFYRRL"
     gene            complement(1019..1648)
                     /locus_tag="P5549_07485"
     CDS             complement(1019..1648)
                     /locus_tag="P5549_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRW12951.1"
                     /translation="MKVYKIPEATVMRLSVYSRYLMQMQEKGVDTISSGEIAAGVGVG
                     SAQVRKDLAYFGEFGTRGVGYKVSELYGHLIKILGLNQTWNVIIIGAGKLGSALALYK
                     GFNDRGFKVRAVLDTNQDIIGEKLADIVVEPLEVMKERILQHNIKVGIITVPAQAAQQ
                     VTDKLLEAGVKAILNFSPEVLRVPSGVILRNVDLSVNLEVLSFNLSFNS"
     gene            complement(1859..2848)
                     /locus_tag="P5549_07490"
     CDS             complement(1859..2848)
                     /locus_tag="P5549_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRW12952.1"
                     /translation="MFSLVWVIVIVVLALIFDYINGFHDTANAIATSVSTKALSPRAA
                     IVVAAVLNFLGALSGTAVAATIGKGIIMPEVVTPQVLCAALMGAIFWNLFTWYFGIPS
                     SSSHALIGGLVGAAMAGFGPGEVSWDGFIKIFSGLLTTPVIGLIVGSLIMYLLFWMFK
                     KSSPAKTNRQFRRLQILTACMASFSHGSNDAQKTMGIITMSLVSAGILTNFEVPLWVK
                     IACALAMAAGTAMGGWKIIRTMGGKIFRIEPINGFAADFSSSLVIFVSSKLGIPVSTT
                     HVVSSSIMGVGAAKRMKGVRWNIAKQIVTAWFITIPLSGLASAMVYMLFQIVF"
     gene            complement(2841..3473)
                     /locus_tag="P5549_07495"
     CDS             complement(2841..3473)
                     /locus_tag="P5549_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRW12953.1"
                     /translation="MGIKLKPQDERFYQYFEDSTEAISEAANILKAFFEHRGDPVMNL
                     ERINEVEHHGDRIFLKTIEQINHSFITPFDREDILLLAQDLNRVLDHIQGTMEKVVIY
                     KVGQPKERYVLDMVNVLVEATEEIKFAVRKLRELRHHHREIIESCERIRSFEHQGDNL
                     YRAGIASLFENTEDVIEIIKWKEVYEHLETTLDYCETVSNVIKGVAVKYV"
     gene            complement(3547..4788)
                     /locus_tag="P5549_07500"
     CDS             complement(3547..4788)
                     /locus_tag="P5549_07500"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRW12954.1"
                     /translation="MARIQRLLQRLHEPQERVAAVHVAGTNGKGSTSLMIADIVSAAG
                     YQVGRFSSPHLHCYEERYTINGTLISTAQLLRYLERIEEQLADWPDDDFPTEFEILTA
                     AAFLYFSEAKVDLAVLEVGLGGLYDSTNVIQPLVAVITGVDYDHMSILGNTLEEIAFN
                     KAGIIKAGAAVVAGAMPDAAERVIIARAEQLQAPLTRAATVRIERTKAPELQGQVIHL
                     TSRRFQLRDQRFALLGTFQLGNLACAVAAVERLIEQGFVIRAEDMTRALANLQLPGRL
                     EVLCSQPLVIGDVAHNPQGAQALSHALQELLPGRKRVLVCGMLDDKDQTASLAALGPF
                     CSQAVFTRPLGERSRDWHRTAGLWKRQFTDIPCFEMENITAAVQKGLALLNGEEYLLI
                     TGSFYVLEEARSYFPNNLTVC"
     gene            complement(4836..7481)
                     /locus_tag="P5549_07505"
     CDS             complement(4836..7481)
                     /locus_tag="P5549_07505"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRW12955.1"
                     /translation="MPTNNLSSVYDPGEVEGKWYDFWKSHNYFAPADDQGQKTFTIVM
                     PPPNVTGQLHLGHALDNTLQDILTRWRRMQGYRTLWLPGTDHAGIATQARVEEALAAE
                     GLSRYDLGREKFLERVWKWKHLYGSRITEQLSLMGSSCDWSRERFTMDEGCSRAVREV
                     FVNLYEKGLIYQGNYIINWCPKCQTAISDIEVEHADSDGHLWHIKYPVEGSDEFLVVA
                     TTRPETMLGDTGVAVHPEDERYAALVGKNVILPLMNRVIPIFADEYVDREFGTGAVKV
                     TPAHDPNDFDMGRRHHLEEIAVINLDGTMNEMAGAYAGLDRYECRRRIVDDLQACDLL
                     LATEDHRHAVGHCQRCDTVIEPLISKQWFVKMKPLAEPAIARVLSGEIKFVPERFTKT
                     YINWMENIRDWCISRQLWWGHRIPVWYCQQCGEIIVSKDDPTACPACAASELMQDEDV
                     LDTWFSSALWPFSTLGWPEQTADLADFYPTSVLVTGRDIIFFWVARMIFSGIAHTGQV
                     PFHEVNIHGLILDGQGRKMSKSLGNGIDPIEVIGKYGADTLRFSMITGVTPGNDVRFH
                     WDKVENSRNFANKIWNASRFVLMNLENFAALELQPEELALADRWILSRLHLKIEETTR
                     LLEGYDLGEAAKGLYEFIWDDFCDWYIELVKPRLSRDADPQQKAVAQNVLRQVLMDIL
                     RLLHPFMPFITEEIYQKLPGCHATIMLDPWPCPDESQLEPAAMEEMQVIMTIIRALRN
                     IRAEFNLSPAAVIPAIVVTSEDKVRQLLQQSGAYIENMARAQLTVTDNLPEKPRQAVS
                     ALTHFAEIYVPLEGMIDIAKEIARLEKDLQTVEKELAKAEGKLQNEHFTAKAPAEVID
                     KEKAKIEEFRSRKEGILKRLEILRV"
     gene            7966..9150
                     /locus_tag="P5549_07510"
     CDS             7966..9150
                     /locus_tag="P5549_07510"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRW12956.1"
                     /translation="MTREVVIVSACRTAVGSFGGSLKDVPVVQLGTVVIKEALQRAGL
                     QGEQVDEVVFGCVLQAAQGQNVARQAMIEAGLPQEITAFTVNKVCASGLRAVSLAAQI
                     IKAGDADIIVAGGMENMSAAPFALDQARYGYRMGKGELIDCMIKDGLWEAFNNYHMGI
                     TAENINDQYGITREEQDEFAFRSQERAAAAIASGRFKEEIVPVVIKSKKGETVFDTDE
                     FPKQTSLEKMATLKPAFKKDGSVTAANASGINDSAAAVVVMSREKADELGIKPLARIA
                     SYASGGVDPKVMGLGPIPATRKALAKAGLTIDDIDLIEANEAFAAQSIAVARDLGFAG
                     KMDKVNVNGGAIAIGHPIGSSGCRILVTLLHEMQKRESRYGLATLCIGGGMGAAVIVE
                     RI"
     gene            9293..11128
                     /locus_tag="P5549_07515"
     CDS             9293..11128
                     /locus_tag="P5549_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12957.1"
                     /translation="MPHSTYLYQTRDIKFQIKEWLDMDKLLSCDGYKDYYGVDDIDSF
                     LEVNAKISRDVICPANKDSDEIGMSFVGGEEQAVITPDSFKNVYNTVMEAGLGPQWGD
                     RQSEGRMPLAWYAPILEQQSGASPAIVMLWCLTQGATTVLQFEGTPKQQELYLDKLFA
                     GEWGGTMCLTEPGAGSDVGACASKAFATEIPGLYKIKGQKCFITSGDHDCVENMIHLV
                     LAKTEGSRAGTAGISLFIVPKFWVNDDGSMGAWNDVTTVGIEHKLGLHGSATCTLSFG
                     ENSNCLGWLVGDAFPVEGRGKGMAQMFRFMNEERLNTGLFSLGALGAAYYAALDYAKV
                     RVQSRKATDPKGSSVRIIEHEDVRRMLMYQKSCMEAIRAMLYQTYYYVDLSHEAADPA
                     EREYADDMFMINNPLCKSFASDMAWILTQEAIQVHGGYGFMEEYAPASLARDCKIYSL
                     WEGTNFIQAQDFTGRKFTMKDGEPFRKWLAEIATFVLVNKSAAFAAEFTMLEEALQAF
                     NSIAALNADWTKNNIPLKQLFATRTLHAAARLYSGKLMLDQALLAAGKLAELGDDHYD
                     ANFYKGKIASARFYVMNVVPEIFAMEKAMKAADTSAVDCPEEAFM"
     gene            complement(11338..>11865)
                     /locus_tag="P5549_07520"
     CDS             complement(11338..>11865)
                     /locus_tag="P5549_07520"
                     /inference="COORDINATES: protein motif:HMM:NF036547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12958.1"
                     /translation="ISATNQSVEELLKQKRFREDLYYRLCVMPIHVPSLSERRDEILP
                     LADIFLRRFNTKYGLQKELSQDVKKWMYNYDWPGNVREVQNIVERLAIVSDSDLIDMD
                     DLARTHVKIGDNADYLSLEDSISLKEASEKLEKILIERASKAHKNSRDAAEALGISQA
                     TYLRKAHKYEVLEND"
     assembly_gap    11866..11875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11876..12761)
                     /locus_tag="P5549_07525"
     CDS             complement(<11876..12761)
                     /locus_tag="P5549_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12959.1"
                     /translation="MSNEQELIKKQARRINELMTLIDFVSDGVLVTDEQSNIIMINKN
                     HVLMEFSTREEIIGKSIPQLVDEGYYVELKPIALPELKEFLLSKQSVNFEMITKLHRR
                     IFSTTQPIMDEFGNIKYIVYTSRDITEIKRLRNELEKEAYLRKLYQQELISKEDVNTF
                     VSESTRMKHIFNIVQKVSQVDSPVFLTGQSGVGKGLIANKIHELSRRGPHPFIQINCA
                     AIPDSLFESEIFGYEEGASTGAKKQGKPGLLELAGEGTVFLDEIADLPLPMQAKFLQV
                     LQEGEFRRVEGTTSIHLRC"
     gene            complement(12878..13042)
                     /locus_tag="P5549_07530"
     CDS             complement(12878..13042)
                     /locus_tag="P5549_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12960.1"
                     /translation="MDKKNGLRIALALVFLIVILIYTGTGGAELIPTLLGFVLGGAGI
                     YFIYKKVLKK"
BASE COUNT         3247 a         3550 c         3129 g         3218 t
ORIGIN      
        1 ccgggggcat gtccttgatc ccaaactggt aatccatcag gcgttttccc tcccagtaaa
       61 ttgacgccct gtttacccag catgatataa agtctgttaa taggcaaacc cacgacatta
      121 aaatagcatc cttcaatttt ttcaatcaaa agggcccctt tgccctggat gccgtatccg
      181 cctgccttgt ccacccattc tccccaattg agataggcat tgatctcccc cggagtgagg
      241 gtgcgaaaat atacctcggt actttccacc ccggtatcac agattccccg gttgctgtcc
      301 agaacgcaaa ggccggtcat aaccttgtga tgcctgccgc ttaaaagaga gagttttcga
      361 aaagcatctt cccggttgac cggtttgccc atgatatgct catccaaaat gaccagggtg
      421 tcggcagcaa tgataatggc atcccgccag acgccggcga cttgttcggc tttgcgccgg
      481 gccagttgct ggaccaattc aggagcggag cattctttca tcggttcttc gtcaacatct
      541 gccgcggctg ccacgaacgg cagaccaatc tgtgcaaaca gctgttgtcg gcgtggggaa
      601 gccgaagcca aaattatttt ctgcaaaatg actccttatt ggtgtccggt tacaagcgtc
      661 tataaaacaa ccatgccagc acgaatccca gaatggtaaa aaagttgatg ttcagcataa
      721 acccaaagct cagcgtgaat acatgcaagt ccatggtaaa ggtgggtaaa cccacactct
      781 ggatcctgcc cagcagagcc agcgccggcc ataatctgac gattgcatcc ccgatccagc
      841 cgccgatgat tccgcccaac gcgagcaaca gcaataaaaa ttgccagccc gggtaagttc
      901 tggtcctgtt tgccaagaca cttcctcccg cattttttca agtttagcat gcccatcttg
      961 taaaaacaag aacggcaaaa gatagaaagc cctccaaaag agagcttttc aggcagtcct
     1021 agctattgaa ggaaagattg aaactgagca cttccaggtt gacggagaga tcgacattgc
     1081 gcaggatcac ccctgacgga actcgcaata cttccggtga aaagttcaga atcgccttta
     1141 cccctgcctc cagcaattta tccgtaacct gttgggcagc ctgggcaggc acggtgataa
     1201 tgcctacttt aatattgtgc tgaaggattc tttccttcat cacttccaat ggttcaacga
     1261 caatatcggc cagtttttcg ccgataatat cctgattggt atccagcacc gcccgcactt
     1321 taaagccccg atcattgaat cctttatata aggccagtgc tgaaccaagt tttccagcac
     1381 cgatgatgat cacattccag gtctggttca atcccagtat tttaataaga tgtccataaa
     1441 gttcactgac tttatagccc actcctcgcg tcccgaattc cccgaaataa gccagatcct
     1501 ttctgacctg ggcggatcct acccctaccc ctgcagcaat ttctcctgac gagatggtat
     1561 ctactccctt ttcctgcatc tgcatcagat aacgagaata aaccgacaaa cgcatcacgg
     1621 tagcttccgg gatcttgtag accttcaacc gtcccactcc ttcaacttca gactctaatc
     1681 ttattatgtt atttaatcgc ccataatgca attagcagta tatattattt ccattattta
     1741 agtatacaga agttctgccg tcattgcagc ttaatggggc ataaaaataa aaaaggcccg
     1801 aacgggccga ggatggtgat gccaatcccc catcctaacc atagattctt tttaatgttt
     1861 aaaacacaat ttggaacagc atataaacca tagccgaagc caaaccggac agcggaatgg
     1921 tgataaacca agcggtgaca atctgcttgg caatgttcca gcgcacaccc ttcattcttt
     1981 tggcggcacc gacgcccatg atggaggacg acacgacatg ggtggtgctg accggtatcc
     2041 ccagtttgct ggaaacaaag ataaccagcg aggaggaaaa gtcggcagcg aacccgttga
     2101 tcggctcaat gcgaaagatc ttgccgccca tggtccgaat aattttccat ccgcccatgg
     2161 ccgttcccgc cgccatggcc agagcgcagg ctattttaac ccagagcgga acttcaaaat
     2221 tggtcagaat cccggcactg accaaactca tggtgataat tcccatggtc ttttgggcat
     2281 cgttggagcc atgggaaaaa gaagccatac aggcggtaag gatctgtaaa cgccggaatt
     2341 gccggttggt cttggccgga gaagatttct taaacatcca gaacaacaga tacataatca
     2401 gactgccgac aatcagcccg ataaccggtg ttgtcagcaa accggaaaat attttaataa
     2461 atccgtccca gctcacttcc ccggggccaa atccggccat cgccgcaccc accagaccac
     2521 cgatcaaggc atgggatgaa ctgctgggga taccgaaata ccacgtaaac agattccaaa
     2581 agatagcgcc catcaaggct gcacacaaca cttgcggcgt cacgacttcc ggcatgatga
     2641 tgcccttgcc gatggtggcc gcaaccgcgg ttccactcag tgcgccaaga aaattgagta
     2701 cagccgctac cacgatggcc gcccgcgggc tgagtgcctt ggtggaaaca gacgtggcaa
     2761 ttgcattggc agtatcatga aagccgttaa tgtaatcgaa tatcaaagcc agaacaacaa
     2821 taacgatgac ccataccaaa ctaaacatat ttaacggcta ctcctttgat cacattgctg
     2881 accgtttcac aataatccag ggtcgtctca agatgctcgt aaacctcttt ccatttgatg
     2941 atctcgatca catcctcggt gttttcaaaa agactggcga taccggctcg atagagattg
     3001 tctccttgat gctcaaacga gcgaattctt tcgcaagatt caatgatttc acggtgatgg
     3061 tgacgcagtt cccgtagttt acggacggca aatttgatct cctcggttgc ttccaccagt
     3121 acgttgacca tatccagcac atatctttcc ttgggctgac caactttgta aataaccact
     3181 ttctccatgg ttccctggat atgatccaat acccggttca gatcctgcgc cagcagaaga
     3241 atatcttccc ggtcaaacgg ggtgataaat gaatggttga tttgttcaat ggttttcaaa
     3301 aaaatgcgat cgccgtgatg ttcgacttca ttaatcctct ccagattcat gaccggatca
     3361 ccccgatgct caaaaaaagc ctttagaatg ttggcggctt cggaaatggc ttctgtgctg
     3421 tcttcaaagt attggtaaaa gcgttcatcc tgtggcttta gtttgattcc caaagagatt
     3481 cctccttgtt attttctctc atccttcctt cattgtcatt atagcaatgt tttggttttc
     3541 gcattgttaa caaactgtta aattatttgg aaaataactg cgcgcctctt ccaaaacata
     3601 gaaagagccg gtgatcaata aatattcctc cccgttcagc agtgccagtc ctttttgcac
     3661 ggcagccgtt atgttttcca tttcaaaaca agggatatca gtaaattgac gcttccacag
     3721 cccggcggtc ctgtgccaat cacggctgcg ctcccccagc gggcgggtaa agaccgcctg
     3781 actgcagaaa gggccgagtg ctgccagact ggccgtctgg tccttgtcat ccagcatccc
     3841 gcaaaccaaa actctttttc gtccgggcag cagttcctgc agggcatggc tcagtgcctg
     3901 cgccccttgc gggttatgag ccacgtcgcc aatgaccagc ggttgactgc acagtacctc
     3961 caaacggcct ggcagttgca ggttggccag cgcgcgagtc atatcttccg cccgaataac
     4021 aaatccctgc tcgatcaatc tttccaccgc cgcaacggca caagcgagat tgccgagttg
     4081 gaacgtaccc agcagggcaa agcgttggtc ccggagttga aaacgccgac tggttagatg
     4141 aatcacctgc ccttgcaatt ccggagcttt agtcctttca atccggaccg tggctgctct
     4201 ggtgagcggt gcctgcagct gttcggcccg ggcaatgatt actctttctg ccgcatccgg
     4261 catggcaccg gcaacgaccg cagctcccgc tttgataatg cccgctttgt taaaagcgat
     4321 ttcctccagg gtgtttccca gaatactcat atgatcataa tcgactccgg tgatcaccgc
     4381 gaccagtggc tgaatgacat tggttgagtc ataaaggcct cccagtccaa cttccaggac
     4441 ggccaggtca acctttgctt ctgaaaaata caaaaaagcc gccgcggtca ggatctcaaa
     4501 ctccgtagga aaatcatcgt ccggccaatc agcaagttgt tcctcgatac gctccagata
     4561 ccgcagcagc tgagccgtac tgattagtgt tccgttgatg gtataacgct cctcataaca
     4621 atgcaggtgg ggtgatgaaa accgccccac ctggtatcct gctgcactga cgatgtctgc
     4681 gatcattagt gaggttgaac ctttgccgtt ggttcccgct acatgtactg cggccacacg
     4741 ttcctgcggc tcatgcaggc gttggagcaa tcgctgtatc cttgccaaac ccggtttgct
     4801 tcccggagcg gccatccgct gcagcttacg ctcaatcaga ctcgtaaaat ctccagacgt
     4861 tttaatatac cctctttccg acttctgaat tcctcaatct tggccttttc cttgtcaata
     4921 acttcggctg gggccttcgc ggtaaaatgc tcgttttgca gtttgccttc cgctttggcc
     4981 agttctttct ctactgtttg cagatccttt tccaaacggg cgatttcctt ggcaatgtcg
     5041 atcattccct ccagcggcac ataaatctct gcaaaatggg tcagggcgga taccgcctga
     5101 cggggtttct ccggcagatt gtccgttacc gtcaattgtg cacgcgccat gttttcaata
     5161 taggcgccgc tttgttgcag caattgccgg actttatcct cagacgtcac gacgatggcc
     5221 ggaatgactg ccgccgggct gagattgaac tcggcccgaa tgttgcgcag cgcacggatg
     5281 atggtcatga tcacctgcat ctcttccatg gctgcgggtt caagctggct ttcatccgga
     5341 caaggccagg gatccagcat gatggttgca tggcagccgg gcagcttttg gtagatttct
     5401 tctgtaataa agggcatgaa tggatgcaac agccgcagga tgtccatcag cacctgccgc
     5461 agcacgttct gcgccaccgc cttttgctgc ggatcagcat cacgggacag acggggtttg
     5521 accagttcaa tataccaatc gcagaagtca tcccagataa actcgtacaa tcctttggcg
     5581 gcttcgccca agtcataacc ttccagcaat cgagtggtct cttcgatttt cagatgcagt
     5641 cgggacagaa tccagcgatc cgccagggcg agttcttccg gctgcagctc gagcgcagca
     5701 aaattctcta gattcatgag tacaaagcgg gatgcattcc agattttgtt ggcaaaattg
     5761 cggctgttct ccaccttgtc ccagtgaaaa cgaacatcat tgccgggcgt aactccggta
     5821 atcatggaaa agcgcagcgt atctgcaccg tattttccaa tcacctcgat gggatcaata
     5881 ccgttgccca aagatttgct catcttcctg ccttgcccgt ccaggatcaa accatgaata
     5941 ttgacctcat ggaaaggcac ctgcccggta tgcgcaatgc cggagaagat catgcgcgcc
     6001 acccagaaga aaataatgtc ccgcccggtg accagcactg aagtggggta gaagtcggcc
     6061 aggtcagcag tctgctcggg ccagccaagg gttgaaaagg gccacagggc gctggagaac
     6121 caggtgtcca gcacatcctc gtcctgcatg agctcactcg ccgcacaagc cggacacgcc
     6181 gtgggatcat ccttgctgac gatgatttca ccgcattgct ggcaatacca gacgggaatc
     6241 cgatgtcccc accacaattg ccgggagatg caccagtcgc gaatgttttc catccagttg
     6301 atataggttt tggtaaagcg ttccggaaca aacttgattt ctcccgacaa cacccgggca
     6361 atggctggct cggccagggg tttcattttt acaaaccatt gtttggaaat cagcggctca
     6421 atgacggtgt cacagcgctg acaatgaccg actgcatgac gatgatcctc ggtcgccagc
     6481 aacaagtcgc aggcctgcag atcatctacg atccgacgcc ggcattcata gcgatccagt
     6541 cctgcataag cgccggccat ttcattcatg gtgccatcca gattgattac cgcaatttct
     6601 tccagatggt gccgccgtcc catatcaaag tcattcggat cgtgggccgg ggtaactttg
     6661 acggctccgg tgccgaattc ccgatccaca tattcatcgg caaagatcgg aatcacccgg
     6721 ttcatgagcg gtaaaatgac atttttaccg accaatgcgg catagcgctc atcctcggga
     6781 tgaaccgcca cgccggtatc acccagcatg gtttccgggc gggtggtggc cactaccagg
     6841 aattcatcgc tgccttccac cggatacttg atgtgccaca gatgcccgtc gctgtcagca
     6901 tgctccactt caatatcgga aatggccgtc tgacacttgg gacaccagtt aatgatatag
     6961 ttgccctgat agatcaggcc tttttcataa agattgacaa aaacttcccg caccgcccgg
     7021 gagcaaccct cgtccatggt aaagcgttcc cggctccaat cacaggaaga acccatcagg
     7081 ctgagttgct cagtgattcg actgccatac aggtgtttcc atttccatac acgttcgagg
     7141 aatttttccc ggcccaaatc ataacggctc aagccttctg ccgccagcgc ctcctccact
     7201 ctggcctggg tagcaatacc ggcatggtcg gtgcccggca gccacagggt acgataaccc
     7261 tgcatgcggc gccagcgggt taggatatcc tgcaacgtat tatccagcgc atgacccaaa
     7321 tgcagctgcc cggttacgtt gggcgggggc atgacgatcg taaatgtttt ttggccttga
     7381 tcgtcggccg gcgcaaaata attatggctt ttccagaagt cgtaccattt cccttcaact
     7441 tccccgggat cgtatacact ggataaattg tttgtcggca tttaattgcg acctcctcat
     7501 caagatctgt gtaaatctcg ctcgctcttt tgtattccaa tacattctaa attctactta
     7561 atttacgccg gccagcgcaa gtttttcgca taaatcaacg caatcataaa tatcgctgcc
     7621 gcaggattga agcaacccgg acgcagcaaa atcccgccaa tacttgtttt ttaatatgca
     7681 tcccgttttt ttgctgcata aacgaagcaa atacgtgccc tttcccggtg ataaggcaca
     7741 aaatattccc ctgtccaaat taaaagtcat ttataagatt ttcaaggatg aagcatagtt
     7801 gattttttta tgcagcaagg tagtgctttt atgtgttctt ctgcttcatt tgcgaatcag
     7861 gccgcccatc ctcctaatat ggcacaaagc ttgctttaac tctgggcaag aacgatgcga
     7921 actgattcat caaaacaaag cgatcgtgaa aggaagtgtt tatttatgac aagagaagtc
     7981 gtaatcgtca gtgcctgtcg taccgctgta ggttctttcg gcggcagcct caaagatgtc
     8041 cccgtcgttc aactgggcac agtggtcatc aaagaagcac tgcaaagagc aggactgcaa
     8101 ggcgaacagg tggatgaagt ggttttcggc tgcgttttgc aagccgcgca gggacagaac
     8161 gtggcccgtc aagcaatgat tgaagccggt ctgccccagg aaataactgc ttttaccgtg
     8221 aacaaggttt gtgcctctgg tttgcgtgca gtcagtctgg ccgcccagat tatcaaggcc
     8281 ggagatgcgg acatcattgt ggccggcggc atggaaaaca tgagcgcagc accgttcgcc
     8341 ctcgatcagg cccgttacgg ttaccgcatg ggcaaaggcg aactaattga ctgcatgatc
     8401 aaggatggtc tatgggaagc attcaacaat tatcacatgg gcattaccgc ggaaaacatc
     8461 aacgatcaat acggcattac ccgcgaagag caggatgaat tcgcattccg cagccaggaa
     8521 agagccgcgg ccgccattgc ctccggcagg ttcaaagaag aaatcgtacc ggtggtaatc
     8581 aaaagtaaaa aaggcgagac ggtttttgac actgacgagt ttcccaagca aaccagtttg
     8641 gaaaaaatgg ccactttgaa accagctttc aaaaaagatg gcagtgtaac cgccgccaat
     8701 gcctccggta ttaatgacag cgccgcagca gtggtagtga tgtccagaga aaaagctgat
     8761 gaattgggga tcaaaccgct ggccagaatc gcaagctatg cctctggcgg ggtggatccc
     8821 aaagttatgg ggctgggccc cattccagcc acccgcaagg cgctggcaaa agccggcctg
     8881 accatcgacg acattgattt gattgaggcc aatgaggcct ttgccgccca atccattgcc
     8941 gtcgccagag atttgggctt tgccggcaag atggacaagg taaatgtaaa cggcggtgcc
     9001 attgccatcg gccatcctat cggttcatcc ggctgccgca tcttggtcac actgctgcat
     9061 gaaatgcaga agagagaatc ccgctacggc ctggctacct tgtgtatcgg cggcggcatg
     9121 ggcgccgcgg tgattgtcga acggatctag gctgccgacg aatttactca accgtcatga
     9181 acgcatagac ccggccggtg cggcggcatt aacaatgcca atcccgaatc ggccagaaaa
     9241 cgtaaatgat aaccagccca cgggaacaac aatttgaaat ggaggaattt atatgccgca
     9301 cagtacgtat ctctaccaaa cccgtgatat caagtttcag atcaaggaat ggctcgacat
     9361 ggacaaactt ctgtcctgtg acggttacaa ggactattat ggcgtggatg atatcgattc
     9421 atttctggag gtcaacgcca agattagccg ggatgtcatc tgcccggcca acaaggactc
     9481 ggatgaaatc ggcatgagct ttgtgggcgg cgaggaacag gcggtcatca cccctgactc
     9541 attcaagaac gtctataaca cggtgatgga agccggacta ggaccacagt ggggcgaccg
     9601 ccagtctgag ggccgtatgc ctctggcctg gtatgcaccg attctggaac agcaatctgg
     9661 agcatctcca gccatcgtca tgctgtggtg tttgacccaa ggggctacta ccgtactcca
     9721 gtttgagggg acccccaaac agcaggagct ctaccttgac aaattgtttg ccggagaatg
     9781 gggcggcacg atgtgcctga ctgaaccggg tgccggttcc gacgttggcg cctgtgccag
     9841 caaggcattt gccacggaaa taccagggct atacaaaata aaaggtcaga agtgctttat
     9901 tacttccgga gaccatgatt gtgtggagaa catgattcat ctggtattgg ccaaaacaga
     9961 gggttccaga gccggtaccg ccggcatttc actctttatc gttcccaagt tctgggtcaa
    10021 tgacgacggt tccatgggtg cctggaacga tgtaaccaca gtgggtatcg aacacaagct
    10081 gggcttgcac ggttccgcca cctgcacctt gtccttcggt gaaaacagca actgcctggg
    10141 ctggctggtg ggcgatgcct tcccagtgga gggacgcggc aaaggaatgg cgcagatgtt
    10201 ccgctttatg aatgaagaaa gactcaacac cggtttgttt tctctggggg cgctgggcgc
    10261 ggcttactat gccgccctgg actatgccaa agtcagggta cagagcagaa aagccaccga
    10321 ccccaaggga tccagtgtgc gaattattga acatgaagat gtgcgccgca tgctgatgta
    10381 ccagaaatct tgcatggaag ccatccgggc catgctctac caaacctatt attatgtaga
    10441 cctctcacac gaagcggctg acccggcgga gcgggaatat gccgatgaca tgtttatgat
    10501 caacaatccg ctgtgcaaat cctttgcttc tgatatggcc tggattctca ctcaggaagc
    10561 catccaggtt cacggcggct acggctttat ggaagaatat gcccccgcct ccctggcccg
    10621 ggactgcaaa atttattctc tctgggaagg caccaacttt attcaggcgc aggacttcac
    10681 tggccgcaaa ttcaccatga aagacggtga acctttcaga aaatggctgg ctgaaatcgc
    10741 gactttcgtg ctggtcaaca aatcggccgc gtttgcagca gaattcacca tgctggagga
    10801 agccctgcag gcgttcaaca gcattgcggc gctgaatgcc gactggacca agaacaacat
    10861 ccccttgaaa cagctctttg ccactcgcac cctgcacgcg gcggcgcgtt tatacagcgg
    10921 caagctgatg cttgaccagg cgctgttggc cgccggcaaa ctggcggaac ttggcgatga
    10981 ccactatgat gccaatttct acaaaggcaa aatcgccagt gcccgcttct atgtcatgaa
    11041 tgttgtcccg gaaatctttg cgatggaaaa agcgatgaag gccgccgata ccagtgcggt
    11101 tgactgccct gaagaagcgt ttatgtaaac acgcaggatg ctgacccttg acgggcagga
    11161 taccttttat aagcaatcat aaagccctgt cctccggttt attcactccc ctccccggag
    11221 ggcagcggct tcgtccccaa acatgttcga agataatcag ccctgagcct ggccggccaa
    11281 cgctgcggat tgacccactg ctgcgttggt gcctggcctc accgggccaa tccgtattta
    11341 atcattttcc agcacttcat atttatgtgc cttgcgcaga taggtggcct gactgatccc
    11401 cagggcttcc gccgcatcgc gcgaattttt atgggctttg ctggctctct cgatgagtat
    11461 cttctccagt ttttcgctgg cctctttcag ggaaatactg tcttccaggc taagatagtc
    11521 ggcattatcg ccgattttca catgggtgcg ggccaggtca tccatgtcaa tcaggtcaga
    11581 atctgataca atcgccaatc gttccactat attctggact tctctgacat tgcctggcca
    11641 gtcatagttg tacatccatt tctttacatc ctgactcagc tctttctgca agccgtattt
    11701 ggtattaaaa cggcgcagaa atatatccgc cagcggcagt atttcatccc ggcgttcact
    11761 taaactgggc acatggatgg gcatcacaca tagacgataa tacaagtcct cccggaagcg
    11821 tttttgttta agcaactctt ccacgctctg attggtcgcc gagatnnnnn nnnnnggcag
    11881 cgcaaatgaa ttgaggtcgt cccttctacc cggcggaatt cgccctcctg cagcacctgc
    11941 aggaatttgg cctgcatggg caacggcaaa tcagcaattt catccaggaa aaccgttccc
    12001 tctccggcca attcaagcaa acctggtttg ccctgctttt tagcgccggt agaggccccc
    12061 tcctcataac caaatatctc cgattcaaac aaggaatccg gtatggcggc acaattgatt
    12121 tgaataaaag gatgtggtcc ccggcggctg agttcgtgaa ttttgttggc aatcaatccc
    12181 ttgccaaccc cggactgccc ggtcagaaaa accggactgt ccacctgact gaccttctga
    12241 acgatgttaa atatatgttt cattctggta ctttcggaaa caaaggtatt tacatcttcc
    12301 ttgctgatca gttcctgctg ataaagcttg cgcaaatagg cttccttttc aagttcgttg
    12361 cgcagtcgtt taatttccgt gatatcccgg gaagtataaa ctatgtattt gatgttaccg
    12421 aattcatcca taattggctg ggtagtactg aaaattctgc ggtgcagctt ggtaatcatt
    12481 tcaaaattaa ctgattgttt gctgagcaag aattctttta gctctggcag ggcaatcggt
    12541 ttcaattcaa cataatagcc ttcgtcaacc agctgaggaa tgcttttgcc aatgatctct
    12601 tcccgtgtgg aaaactccat caaaacatga ttcttgttga tcatgataat gttgctctgc
    12661 tcgtcggtca ccagcacccc gtcgctgaca aaatcaatga gggtcatcag ttcattgatt
    12721 cttcgggctt gctttttaat cagttcttgt tcgttcgaca tacttaccta cccttgttgc
    12781 ctcaaaacag tgtaaaaagc gtcgtttaga tgttgttccc ggaggtatgc gctgccccac
    12841 cggtctgccc ggcagcaagg cagttggtga tgatgcttta tttcttcaac acttttttat
    12901 aaatgaaata aatccctgct ccgcccagta caaatcccag cagagttgga atgagctccg
    12961 caccgccggt tcccgtatag atcaggatga caataagaaa caccagcgcc agtgcaattc
    13021 tcaacccatt tttcttgtcc atctttttct ttcatcctcc tgcagccaat ctcttttgcg
    13081 tgccgttgcg cacctatttc tctgattatt gtactataac cccgagcagc agggaagcaa
    13141 agtcagcaga caaa
//
LOCUS       DAOTHZ010000058        12947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5758_length_12947_cov_7.341064, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000058 DAOTHZ010000000
VERSION     DAOTHZ010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5758_length_12947_cov_7.341064"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="P5549_07535"
     CDS             complement(<1..1001)
                     /locus_tag="P5549_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW12961.1"
                     /translation="MTFGFTYNEDQLALQRTVRKFVENEIVPVRAAYDESEEFPWPIV
                     RKMADLGLNCMNAPERYNGMEFGHIGLCMVIEELSRGCLAIAMTPKVNLLASEPVLYG
                     GTEEQWDYWFPGLCEEGQLAAYAVTEAGAGSDVAGITTSCKRDGDYYILNGTKIFISN
                     GKHAEKLTVLATHDKSKGNRAQSFFMVDTKTDGVSFGKSEHKMGLRCSETSEIILDHV
                     KVHKKYRLGEEGDGFKLAMKSFNSSRPFVASAATGLATGAFEFARDYARERKAFGRSI
                     GDFQGIQFMLADMAMEIEAARLLWQKAAWLLDEKMMAADLSAMSKCYAADMANRVAA"
     gene            1562..2560
                     /locus_tag="P5549_07540"
     CDS             1562..2560
                     /locus_tag="P5549_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW12962.1"
                     /translation="MPITRRSFLKRAAVSGISAATIMTGAGKAAAASVAEGNYALLHD
                     LEKCDGCSHLDTPLCVSACRLKNQTRFPEPEKPIGDYWPQKSHEDWSEKRNLTNRLTP
                     YNWTYVQHLEVNHNGRLVKVHIPRKCMHCDLPPCSTLCPFGVNDKMPEGPVVINPNGC
                     FGGAKCRDVCPWHIPQRQGGVGLYLKIAPTYAGGGVMYKCDMCYDLLQKGEKPACASA
                     CPRGAIRFGDKEELRAYAHQWAQENNGYVYGDTENGGTSNFYLSKVPFEAINQAIMEQ
                     DWDGKPGKINMAVNSKNPLDEPDKMMSGFMLAPVAGIMAAGLTAYNIMKGGDEDEN"
     gene            2550..3290
                     /locus_tag="P5549_07545"
     CDS             2550..3290
                     /locus_tag="P5549_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRW12963.1"
                     /translation="MKIKRHHLAIRFTHWITALSILVLFFSGFGQMPLYKRYFVDQLP
                     GLAWSSDFWVTLHLHYAAAIVLVFISIYYVTYLIATKKYDILPRQGDFKESLQIFKAM
                     VGLAQEPESDKYLAEQRLAFAVTAFSIFALIVTGILKVYKNTVGASYSPDTIFWLAQI
                     HNLFTVILLFSVIVHVLAFLLKPNRPLVISMFTGKIDRDYVIHRHGKWWRKLNPNTTV
                     PEDTTEADAPAIAAEVPPDVLPEPGGSE"
     gene            3442..4299
                     /locus_tag="P5549_07550"
     CDS             3442..4299
                     /locus_tag="P5549_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRW12964.1"
                     /translation="MAIKVISDSTSYIDPDTQKELDIELIPLSVNFPDEQFLETEVDY
                     DYFYNKIEQTGVIPTSSQPSLGDIIEKFREIAAHGDDILAIFISSAMSGTYETALKAR
                     EKVLQEFPQVQIDIMDSYTNCMALGMQVVAAAREIKQGASLHTALQAAQYVRERVRFY
                     FVPETLKYLKKGGRIGTASALLGTILNIRPILTVDMKTGMTHLMEKTRGTSAAIKRML
                     KFLEEDWEKYGLREILIHHINAPEKAENLRDMLMERYDMQVDIRSIGPVIGLHTGLGT
                     VGFVYCTEK"
     gene            4775..6706
                     /locus_tag="P5549_07555"
     CDS             4775..6706
                     /locus_tag="P5549_07555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW12965.1"
                     /translation="MDSTPVFTFILFFANLGLGIYAISRDPRSALNRTFLALIASTVL
                     WVGAYNLVFTLASSAAGIWFWYKIASPGWISLPFFCVYFALVLTGRINSMPKRSLVFI
                     SLPIAVEIILMFTIVHPSMLTDISRYMLTDFISPRSDWFWYLHTPYYLLYSTMMLIII
                     AQWGRSSMYQREKRQARIIVRAGLLLLGCFLLQYSLLPSFGILLPWLVPMAGLLWLVA
                     ILYAITRYKLMDLQKALSLDDILSRSTDIILLLNRSDQIMLANHKAQEVLAYPAKKLP
                     HLSLAEIIPEGFPDTAMIKQKDLTMEKEVFIQTARGELIPVKASIATIKDDLQDTVGL
                     LFIGQDLRLPKQLEQKIAEMKNVESALRESEAKYRNIFENAYDMIYMHDVNGRYLSVN
                     KASEKLLGYSEDELVHMNALHFVVPEQRQRVIDLFLEKRTEHQLVYYEVTIINRYGEK
                     FDLNVSRQLIFETPEETVYQCIARDMTERKQMEEKLKYLSMHDAMTGLYNRAYFTEEL
                     KRMESGRHEPSSIILCDLDYLKKVNDNYGHAAGDEMIIAAAQLIKSCFRSNDVVARFG
                     GDEFAIVVPKSNDAICLHLVEKIQTAIDAHNQTSPFIISISIGYATRTRVEQPMQEVF
                     QQADQAMYADKAQRKKLRS"
     gene            complement(6723..6884)
                     /locus_tag="P5549_07560"
     CDS             complement(6723..6884)
                     /locus_tag="P5549_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12966.1"
                     /translation="MQDLIEVLNVCFWGDEGRGLLAGLENREHDLLRQYIRKKPDEWR
                     SDSGWEEWL"
     gene            6934..7158
                     /locus_tag="P5549_07565"
     CDS             6934..7158
                     /locus_tag="P5549_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW12967.1"
                     /translation="MGKNLKLKSARAALDMSQKDIADAVGVTRQTINAIENGDYNPTI
                     KLCTAICKVLGKTLNDIFWEDEADEKKPVG"
     gene            7136..7564
                     /locus_tag="P5549_07570"
     CDS             7136..7564
                     /locus_tag="P5549_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12968.1"
                     /translation="MKKNQLDEMQVQKRNSIGNQAFLLLMYLLMIDAGLYGFGFRWIE
                     YPANIMIMVTVCAGIYLIRLINNHAYVGPSAENKRPVLRFILMVAIACSVAAGISMML
                     KNASFTNTAQINEMSAPIMFITAAIAIIIALATLLIKRSQ"
     gene            complement(7628..8314)
                     /locus_tag="P5549_07575"
     CDS             complement(7628..8314)
                     /locus_tag="P5549_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRW12969.1"
                     /translation="MELIYTGKTKDVYRQGDGNYLLKFKDDATGENGVFDPGSNTVGL
                     TIAGAGRAGLRLTKHFFEVLQAQGIPTHYIDADVEAATMTVKPAVLFGKGLEVICRLR
                     AVGSFLRRYGDYVEDGQALDYFVETTLKDDAREDPPITEDALDMLGIASKAEYQVLKD
                     LTQRITAVVKAELARKGIELYDIKFEFGRVGQDQQIVLVDEISGGNMRAFKDGRHIQP
                     LELEKMMLAD"
     gene            8564..>9364
                     /locus_tag="P5549_07580"
     CDS             8564..>9364
                     /locus_tag="P5549_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12970.1"
                     /translation="MSSIYETHFWRENFEWKTPHSINYPKMPAYAILRNSSVYKPDKV
                     ATWFYGAEITYNELYLKVTRLANVLAENGVEKGDRVGMLMPNCPQGIIAFWAILMAGG
                     IVVNLNVLYTKDELRFLFDDTNMKAIIVYDAILPLVRELNQASAQPIKTVFVTKLFDF
                     MAGTPKSSAADLGLKDGELHFSEVLETDKIWAPPLVDIQHDDPAVIQFTGGTTGAPKG
                     AVLTQYNIVATAFIANRWCSDFVDNQTYNRRRTLSILPYSHIYGEACGI"
     assembly_gap    9365..9464
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9465..9984
                     /locus_tag="P5549_07585"
     CDS             <9465..9984
                     /locus_tag="P5549_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW12971.1"
                     /translation="DLRIVGPDGNDVPLGEEGEIWIKGPIVMKEYWNRPEETAAAFED
                     GWLRTGDIAYMDEDGYVFIVDRIKDLIIAGGYNIYPREVDEALLKHPKVADAMVIGLP
                     DEYRGETVKAYVQVYPGETLTAEELIAFCRESLAAYKVPHQVEFRDSLPRSATGKALR
                     RILKEEEKQKRG"
     gene            10184..10795
                     /locus_tag="P5549_07590"
     CDS             10184..10795
                     /locus_tag="P5549_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRW12972.1"
                     /translation="MTDNKHKKKLTNRDLQAIETKKRIYDTAVRLLAEKGYDNTSVDD
                     ICNASGVAKGSFYHYFRKKSDIIVKTYEDVDERISQEFDSLPPETTVFEKIVYAPMFQ
                     ARYAIEKSLNYTTQIYKQQIDTENAFFGSDKRAFISFIRSAIQEGQEQGLIRTDMTAD
                     ELSKLVVSYSRGITYDWCLQNGNYDLIERMDKAMRMMIHSFMV"
     gene            11029..12672
                     /locus_tag="P5549_07595"
     CDS             11029..12672
                     /locus_tag="P5549_07595"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HRW12973.1"
                     /translation="MESIGYQMQLTLKRFLDYGMIYAPNQIMKYRDEFEYTYTDHYQR
                     VKQLSNALKSLGVEPGQTVGMLEWDTYRYLEAHWAIPLYGSLFHTVNVRMAPDQIAYC
                     INHAEDVVLFINEDFLPLVESFKDELKTVKAFVIMSDKKDYQPSTSLPNVYEYEALLK
                     QQSADYEFPEISENSRATLCYTSGTTGNPKGVVFTHRELFMHAISVCMTWGLYPNGIE
                     FKANQVYMPLTPMFHVQAWTVPYYAFMLGSKFIFPGKYEPDKILRWLAEEKVDFSHCV
                     TTILHMLVFHPDAEKYDLSNWRVGLGGAKLNRQLAERAWELGIFTQSAYGMTETCPAM
                     TLGQLKPDHLDLPVEDKITYLIKSGIPWIFSQAKVVDDDFSELPRDGKSVGNVVVRTP
                     WCTHEYFHEPDKTRELWKNDWLNTGDVGYIDPEGYLMITDRNKDAIKSGGEWISTLTL
                     EDAVSQFKPVLECAVIGIPHPRYDERPCACIALKPGEIATAEDVRDHLLKFAEEGKIE
                     KWWIPDIPDGYLFLEAIPHNYIGKIEKTTLRKMYAEMKK"
BASE COUNT         3435 a         3161 c         2913 g         3338 t
ORIGIN      
        1 ttggcagcaa cccggttggc catatcggca gcatagcact tgctcatcgc cgacaggtcc
       61 gccgccatca ttttttcatc cagcaaccag gctgccttct gccacaataa gcgagccgct
      121 tcaatttcca tggccatatc cgccaacata aactgaatcc cctgaaaatc cccgatgctc
      181 cggccgaaag cttttctctc ccgggcatag tcacgggcaa attcgaacgc tccggtagcc
      241 aggccagtag cagccgaagc tacaaaagga cgggaagagt tgaaggattt catggccagc
      301 ttaaaaccat ccccctcttc ccccagtcga tattttttgt gtactttcac atgatccaaa
      361 atgatttctg aagtttccga acagcgcagc cccattttat gctcgctctt gccaaaggaa
      421 acaccgtccg tctttgtgtc taccataaaa aagctctgcg cccggttgcc tttgcttttg
      481 tcatgggtag ccagcactgt cagcttttca gcgtgtttgc cgttgctgat gaatattttg
      541 gtgccgttga gaatataata atccccatct cttttacaac tcgttgtaat accggccaca
      601 tcagagcctg ccccggcctc agttactgca taagctgcga gctggccttc ctcacataaa
      661 cccggaaacc agtaatccca ttgttcctcg gtacctccat agagaaccgg ttctgaggcc
      721 agcaaattga ctttgggcgt catggcaatc gccaggcagc cgcggctcag ctcctcaatc
      781 accatacaca agccgatatg gccaaactcc ataccgttgt atctttcggg tgcattcatg
      841 cagttcagcc ccaggtcagc catttttcgt acgatcggcc agggaaattc ttccgactca
      901 tcgtaggccg cccgtacagg tacaatctca ttctcaacaa attttctaac cgtccgctgc
      961 aaagccaact ggtcctcgtt atacgtaaag ccaaaagtca tgttcttcct ccccgcaata
     1021 gataattcta ttagatgtct gtttaaacag gactgcaaga acagcattgc ctgaacagcc
     1081 ttgccttgtt ccctgactgc agttgtttac catgaaatct ctatacataa tgagagaata
     1141 cccttcccaa gccaaccacc ggtttgaacc tgtcaccgga tgattcctcc tctcccttct
     1201 atttcctggg attacaaggt ttctcatcat tgatataacg ttatgtgtga attattacat
     1261 ttcgtaattt taaaatgtaa tataaaacca attatatttc attatgtaat tatttcaact
     1321 attttttaac atcttgtgaa tttggatgat tcctttgttt tgttttgtct tgccttgcca
     1381 tggcaagtcc tcctcactct gcggctcgct tccgacgttc agcatcacac accatctgta
     1441 gctactgtaa ttcagcaacg gggagctgga aaagcaaatt ccctcttgcc aaaatcatta
     1501 tctgcgcata caattaaaaa catcatatac ctaccccggg tataataaag gaggaacaaa
     1561 gatgcccatt acgcgaaggt catttcttaa aagagcggcg gtatcaggga ttagtgcagc
     1621 cacgattatg accggggccg gcaaggcagc agctgcatcg gttgcggaag gaaactatgc
     1681 gcttttgcat gatctggaga agtgtgacgg ctgcagtcat ttggacactc cattgtgtgt
     1741 ctctgcctgc agattgaaaa atcagacccg atttccggag ccggaaaaac ctattggcga
     1801 ttattggccg caaaagtccc atgaggactg gtcggaaaaa agaaacctca ccaatcgtct
     1861 gacgccctac aactggacgt atgtacaaca tttggaggtg aatcataacg ggcggctggt
     1921 caaggttcac ataccccgca agtgtatgca ttgtgacctt cctccctgtt ccactttatg
     1981 tcccttcggg gtcaatgata aaatgccgga agggccggta gtcatcaatc ccaacggctg
     2041 ttttggcggc gccaaatgcc gggatgtctg tccgtggcat atcccccagc gtcaaggagg
     2101 cgtaggtttg tatttaaaaa tcgcacccac ctacgcgggc ggtggtgtca tgtataaatg
     2161 cgacatgtgt tacgatctcc tgcaaaaagg agaaaagccg gcctgtgcaa gcgcttgtcc
     2221 ccgcggcgcg atccgttttg gcgacaagga agagttacgg gcttatgcac atcagtgggc
     2281 gcaggaaaac aacggctatg tgtatggcga taccgaaaat ggaggaacat ccaactttta
     2341 tttgtccaaa gtcccctttg aagctatcaa tcaagccatc atggaacaag attgggacgg
     2401 taaaccaggt aaaatcaaca tggcggtcaa tagtaagaat cccttggatg aaccagacaa
     2461 gatgatgagc ggctttatgc tggctcccgt agccgggatt atggctgccg ggttaaccgc
     2521 ctataatatc atgaaaggcg gggacgaaga tgaaaattaa acgccaccat ctggccattc
     2581 gttttaccca ttggataacg gcattgtcca ttctggtgct gtttttctcc gggtttggac
     2641 aaatgcccct atataaaagg tactttgttg atcagctgcc gggtctggcc tggtcgagtg
     2701 atttttgggt aaccttgcac ctgcattacg cggcagccat cgtgcttgtc tttattagta
     2761 tttactatgt tacctatttg attgctacca aaaaatatga catcctgccc cgccaaggtg
     2821 attttaaaga atcgctgcag atttttaaag ccatggttgg gctggcccaa gaacctgaaa
     2881 gtgacaaata cctggcagaa caaaggctgg cgtttgcagt aaccgctttt tctattttcg
     2941 ccttgattgt cacggggata ctgaaggtat ataaaaatac ggtcggagca agttattctc
     3001 cggatacgat cttctggctg gcgcaaattc acaatctttt taccgtgatt ttgttattct
     3061 cggtgattgt gcatgttctc gcgtttttgc tgaagcccaa tcgtcccctg gtgataagta
     3121 tgtttaccgg caaaatcgac cgggactatg tcatccaccg gcatgggaaa tggtggagaa
     3181 agctcaaccc gaataccacc gtcccggaag acacgacaga agcagatgcc ccggcaatcg
     3241 cggccgaagt gccgccggac gtcctcccgg aaccaggcgg gtcagaataa tggcaatata
     3301 acccattggc acaggataca actatttccc cgggttgcat aaacccgggg aaacaagcat
     3361 tcgacatgtt tggttgcgct catgcatgtt tattcatata atggaaacat tagagaataa
     3421 acggagagga tgtagaaccg aatggccatc aaagtaatca gcgacagcac atcgtatatt
     3481 gatccggata cgcaaaaaga attggacatt gaactgattc cgctcagtgt caattttcct
     3541 gatgaacaat tcctggaaac cgaggtggat tacgattatt tttacaacaa aattgagcaa
     3601 accggcgtca tcccaacctc ttcccagccc tccctgggag atatcataga aaaattcagg
     3661 gaaatcgctg cccacggaga cgatatcctg gccatcttta tttcatccgc catgagcggc
     3721 acttatgaaa ctgcgttgaa agcccgggaa aaagttttac aggaattccc ccaggtgcaa
     3781 atcgatatta tggattccta taccaactgc atggcactgg gaatgcaagt cgtagctgca
     3841 gcccgcgaga taaaacaagg ggccagtctg cacaccgcac ttcaagcagc ccaatatgtg
     3901 cgggaacggg tacgctttta ttttgtgccg gaaacattaa aatatctgaa aaaaggtggt
     3961 cgcataggca ctgcctccgc cctgctggga acgattttaa atatacgtcc gattctcacc
     4021 gtggacatga aaacaggcat gacccatctg atggaaaaga cccgcggtac atcagcggcc
     4081 atcaaacgta tgctgaaatt cctggaagaa gattgggaaa aatacggttt gcgggagatc
     4141 ctcatccatc atatcaatgc tccggaaaaa gccgaaaatc tacgggacat gctgatggaa
     4201 cgatatgaca tgcaggtgga catccgttcc atcggaccgg tgatcggact ccataccggc
     4261 ttgggaacag tcggttttgt ttactgtacg gaaaagtaat ctgcatttgt accgatgatg
     4321 atccgggtat ctggcccgga tcattttttt tgccaggcat aaaaatgttt ccgtttatgc
     4381 aaaggaagaa ttcgtccata ttcgcgatct gtttgactat gctttcattt atgttatgat
     4441 aattctgttg ccgcactatt tgtttgaata tttcatcttt ttaatcgcaa ttggtttgga
     4501 ggtatgatta tggcaggaaa tgtaaaagta atgatctcgc ccagtcgcta cgtgcaagga
     4561 gcaggtgcat tttaatctgt gttatgatag gtgagtcaga agctaattct gactcgcttt
     4621 ttcataaaac caacatttta tattttttca tagtaaaatg aatactgaga aattttttaa
     4681 accttcgcac ttgtttttgc acacattcat gttatttcaa tgcaacaatt accatagtaa
     4741 tagattatct atttcccaga aatcgggtga tttaatggac agcaccccag tctttacctt
     4801 catactgttt ttcgctaatt tgggtctggg tatttatgcc atcagtcggg atccccgttc
     4861 agcgctaaac cggacttttt tggccttgat cgccagtacg gttttatggg tcggtgccta
     4921 caatctcgtt tttactctgg cctcatctgc agccgggatc tggttctggt acaaaatcgc
     4981 ttccccgggt tggatcagtc tgcccttttt ttgcgtctat tttgcgcttg tgctgaccgg
     5041 cagaataaac tccatgccca agcgcagcct cgtcttcatt tctcttccca tagcagttga
     5101 aatcatcttg atgtttacca ttgttcaccc gtcaatgctc accgatatca gccgatatat
     5161 gttgactgat tttatcagtc cccgcagtga ctggttctgg tatctgcata cgccttatta
     5221 tcttctttat tccaccatga tgttaatcat tatcgcccag tgggggcgca gttccatgta
     5281 tcaacgcgaa aaacgccagg cccggataat tgttagggcc ggtttgctgc ttctgggttg
     5341 cttcctgctg caatatagcc tgttgccctc gttcgggatt ttgttacctt ggttggtacc
     5401 tatggccgga ctgctatggt tggttgccat tctttatgcc atcacccgct acaaactaat
     5461 ggacttgcaa aaagcattgt ctttggacga catcctgtcc cgttccactg acatcatcct
     5521 gttgctgaac cgctctgacc aaatcatgct ggccaaccat aaagcccagg aagtactggc
     5581 ctaccctgcg aaaaaactgc cgcacctgtc cctggcagaa attatccctg aaggattccc
     5641 cgatacagcc atgatcaagc aaaaagactt gaccatggaa aaagaagttt tcatacaaac
     5701 tgcccgcggt gaattaattc cggtaaaagc aagtatcgct acgatcaaag atgacctgca
     5761 ggatactgtc gggctcctgt tcatcggaca ggatctgcgt ctgcccaagc agctggaaca
     5821 aaaaatagcc gaaatgaaaa atgtggaatc cgctttgcgg gagagtgagg ccaagtatcg
     5881 caatattttt gaaaatgctt acgatatgat ttatatgcac gatgtcaatg gcaggtatct
     5941 gtccgtcaac aaagcttcag aaaagcttct tggttattcc gaggatgaat tggtccatat
     6001 gaatgcgctt cattttgtcg tccctgaaca acgacaaaga gtgattgatt tgttcctgga
     6061 aaaaagaacc gagcaccagc tggtttacta tgaagtcacc ataatcaacc gatacggtga
     6121 aaagtttgat ttgaatgtca gtcggcagtt gatttttgag accccggaag aaacggttta
     6181 tcaatgtatt gcccgcgaca tgacagaacg caagcaaatg gaggaaaaac tcaaatacct
     6241 cagcatgcat gatgccatga ccggtcttta caatcgggcc tattttacgg aggaattaaa
     6301 acggatggaa agcggacggc atgaaccctc cagcatcatc ctctgcgatt tggactatct
     6361 gaagaaagtc aatgacaact atggtcatgc tgccggggat gaaatgatta ttgctgccgc
     6421 tcagctgatc aagtcatgtt tccgcagcaa tgatgtcgtg gccagatttg gcggagatga
     6481 gtttgccatt gttgtgccga aaagcaacga tgccatctgt ttgcatctgg ttgaaaaaat
     6541 tcaaactgcc attgacgcgc acaaccaaac cagtcctttc attataagca tctctatcgg
     6601 atatgccacg cgcaccagag tcgagcaacc catgcaggag gtttttcagc aagctgatca
     6661 ggccatgtac gccgacaaag cccagcgcaa aaagctccgc agctgatggt tccaattgag
     6721 tgttacagcc attcctccca accgctatca gaccgccatt catccggctt tttccggata
     6781 tattggcgca acagatcatg ttcccggttt tccaatcctg ccagcaaccc cctgccttca
     6841 tcgccccaaa agcagacatt cagaacttca atcaaatcct gcaaactgtg gacttgctga
     6901 cagttggttt acaacaacgt tggaggtggg agattgggca agaatctgaa actaaagtcc
     6961 gcgcgggcgg ctctggacat gtcgcaaaaa gacatcgcag atgcagtggg ggtgacccgc
     7021 cagaccatca atgccatcga aaacggtgac tacaacccca ccatcaaact ttgtacagcc
     7081 atctgtaaag tattgggcaa aacgctcaac gatatttttt gggaggatga ggctgatgaa
     7141 aaaaaaccag ttggatgaaa tgcaggtaca aaaaagaaac agtattggca accaggcctt
     7201 ccttttgctg atgtatctgt taatgatcga tgcggggctt tatggattcg ggttcagatg
     7261 gatcgagtat cctgccaata ttatgattat ggttaccgtt tgtgcgggta tatatttgat
     7321 ccgcctgatc aacaaccacg cttatgtcgg gccatctgca gaaaacaaaa gaccggtttt
     7381 acgattcata ctaatggtgg cgattgcctg ttcggttgcg gcagggatca gtatgatgct
     7441 caaaaacgct tctttcacca acaccgccca gatcaatgaa atgagtgcgc cgatcatgtt
     7501 catcactgct gccatcgcca tcatcatcgc tctggcaaca cttctcatca agcggtcaca
     7561 ataagggcaa aaaggggttg cagttcaccc tgcaacccct taatcttttg acgattggcc
     7621 tgatcggtta atccgccagc atcatttttt ccagttcgag cggctgaatg tgtcggccat
     7681 ccttgaaggc cctcatattg ccgccggaaa tttcatccac cagcacaatt tgctggtctt
     7741 gtccaacccg gccaaactca aatttgatgt catacagttc aatgcccttc ctggccagct
     7801 ctgccttcac cacggcggta atccgttggg ttaaatcctt gagaacctgg tactcagcct
     7861 tggaggcaat gcccagcatg tccagggcat cttcggtaat gggcggatcc tcgcgcgcat
     7921 catctttcag tgtcgtctcc acaaaataat ccaatgcctg tccgtcctct acatagtctc
     7981 catagcggcg caggaaactg cctaccgccc ggagccggca gatcacttcc aagcctttgc
     8041 cgaagagaac ggcgggcttg acggtcatgg tggctgcttc aacatcggcg tcgatataat
     8101 gggtcggaat accttgtgcc tgtaatactt caaaaaaatg cttggtcaga cgcagtccgg
     8161 cccgaccagc cccggcaatg gtcagtccca ccgtgttgga acccgggtca aatacgccgt
     8221 tttcgccagt cgcatcgtct ttgaacttga gtaaataatt gccgtctcct tgacggtaga
     8281 catccttggt ttttccagta taaatcagct ccatggtcac gttcccttct ttgtttttgt
     8341 tattcccgga ataagtcttt ttttccttgc acgggttgcc acataattat atcacttgtt
     8401 ttctcattcc gtataaactt gcgcggaaac cctgttcaac ttttttcgtc agaattttca
     8461 gaattttgtt gcatttcaag ctacctatct ttaaaatgat tcttagatac atcgccagcc
     8521 aggcatagat gtattgatca ataattttcg agagggtgat tcaatgtcat caatttatga
     8581 aactcatttt tggcgcgaaa actttgaatg gaaaaccccg cattcgatca actaccccaa
     8641 aatgcctgcc tatgccattt taagaaattc cagcgtttac aaacccgaca aggttgctac
     8701 ctggttctat ggtgccgaaa taacctacaa tgaactctat ttgaaagtca ctcggctggc
     8761 caatgtgctg gcggaaaacg gtgtcgaaaa aggcgaccgg gtaggtatgc tgatgcccaa
     8821 ctgcccgcaa ggcatcatcg ctttttgggc gatcctgatg gcgggcggga tcgttgtcaa
     8881 cctgaatgta ttgtacacca aagacgaact gcgtttcctg tttgacgata ccaacatgaa
     8941 agcaatcatc gtttatgatg ccatcctgcc cttggtcagg gaactgaacc aggcgagtgc
     9001 ccagcccata aaaacggtct tcgttaccaa actattcgat ttcatggccg gcacacccaa
     9061 gagcagcgcc gctgatctgg gactgaaaga cggtgaattg catttcagtg aagtgctgga
     9121 aacggataaa atatgggccc ctccgttggt tgacatccaa catgacgatc cagccgttat
     9181 tcagtttacg ggaggtacca ccggtgcgcc caaaggagcc gtcctgaccc aatataacat
     9241 tgtagccacc gctttcatcg cgaacagatg gtgttcggac tttgttgaca accagacata
     9301 taatcgccgg cgaacgctga gcatcctgcc ctattcccac atttacggag aagcttgcgg
     9361 aatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgattt gcgaatcgtc
     9481 gggccggatg gaaatgatgt accgctgggc gaagaaggcg agatctggat caaggggccg
     9541 atcgtcatga aagaatattg gaatcgtccg gaagaaactg ctgctgcctt cgaagatggc
     9601 tggctgcgca ccggtgatat cgcttatatg gacgaagatg gatatgtctt tattgtagac
     9661 cgcatcaagg atctgatcat cgccggcgga tataacatct atccccggga agtggatgag
     9721 gcattgctga aacatccaaa agttgccgat gccatggtga tcggccttcc cgatgaatat
     9781 cgcggcgaaa cagtgaaagc ttatgtacag gtgtatccgg gtgaaacgct gaccgccgaa
     9841 gaattaattg ccttttgccg tgaatctttg gctgcctaca aggttcccca tcaggttgaa
     9901 ttcagagatt ccctgccccg cagtgcaacc ggcaaagccc tgcggcggat tttaaaagag
     9961 gaagaaaaac aaaaacgcgg ataaaaacat cccgcctccc tttattacta gtatattcat
    10021 taccaaagca taaaaataaa ctcacccacc gccagagcga gaaaaagatc ggggctgcaa
    10081 tccccggtct tttctttagt agcatcaagc aatacccgca tcaactgacg gaaaggacaa
    10141 aatgtgttag aataccggta atatagttga cgaggatgga agaatgacgg acaacaagca
    10201 caaaaagaaa ttgaccaacc gggatctgca ggccattgag accaaaaaaa gaatctatga
    10261 taccgcagtc cgtctgttgg ccgaaaaagg gtatgacaat accagcgttg acgatatctg
    10321 caatgcctct ggtgtagcca aaggttcttt ctaccattat tttagaaaga aatcggacat
    10381 tattgtaaaa acctatgaag atgtggatga acgcatcagt caggaatttg attcattgcc
    10441 gccggaaacc acggtttttg aaaaaatcgt ttacgcgccc atgtttcaag cccgttacgc
    10501 gattgaaaaa agtttgaact ataccaccca gatctataag cagcaaatcg acaccgagaa
    10561 tgcgtttttt ggttcggaca agcgcgcttt catttctttt attcgcagtg ctatacagga
    10621 aggacaggag cagggactga ttcgcacgga tatgactgcg gatgaactgt ccaagttggt
    10681 ggtttcctac tcgcgcggga ttacgtatga ctggtgtctg caaaatggca attatgactt
    10741 gatcgagcgc atggataaag ccatgcgcat gatgatacac agttttatgg tttaagcctc
    10801 tctcaaaaat tcagcaaaaa actgcggcct tcacggccgt ttttgtgact gcaaatcaat
    10861 ctccaactcg tccaacctcc ttgaaattaa ttagaccaca agtcattgac cgtcagtcta
    10921 tttttggttt aaaattgtat tacatactgt catcccggat cagcaatact attgtcagca
    10981 ccccgaaaac aatgtagcga tgtcaatgca gcgaaggagg ttacgagatt ggaaagtatc
    11041 ggctatcaaa tgcagctcac gttgaaaaga tttttagact acggaatgat ctatgccccc
    11101 aaccaaatca tgaagtaccg ggatgaattc gagtacacct acactgacca ttaccaacgg
    11161 gtcaaacagc tgtccaacgc actgaaaagc ctgggagtgg aaccgggaca aacggttggc
    11221 atgctggaat gggataccta ccgttatctg gaagcccatt gggccatccc tctctacggc
    11281 agtctgttcc acaccgtcaa tgtccgcatg gcgcctgacc agatcgccta ctgcatcaac
    11341 cacgccgagg atgttgtcct gtttatcaac gaagacttcc tgccactggt agagagcttt
    11401 aaggatgagc ttaaaacggt caaagccttt gtcatcatgt ccgataaaaa ggattatcaa
    11461 ccgtcgactt ccctgcctaa tgtttacgag tatgaagccc tgctcaagca acaatccgca
    11521 gactatgagt tcccggaaat atcggagaat tcccgtgcca ccctgtgtta cacatctggc
    11581 accaccggca atcccaaagg ggtcgtcttt acccatcggg aactgttcat gcatgccatt
    11641 tccgtctgta tgacctgggg actgtacccc aacggtatcg aattcaaagc caatcaggtc
    11701 tatatgccgc tgacccctat gttccatgtt caagcctgga cggttccata ctatgctttc
    11761 atgttgggca gcaagtttat ttttccaggt aaatatgaac ctgacaagat tcttcgctgg
    11821 ctggccgaag agaaagtcga tttcagccat tgcgtaacta ccatccttca tatgctggtc
    11881 ttccatcccg atgcggaaaa atatgacctc agcaactggc gggtcggtct gggcggcgcc
    11941 aaactcaacc ggcaactggc cgaaagagca tgggaactgg ggatcttcac ccaatcggcc
    12001 tacggtatga ccgaaacctg tccggccatg accctgggac aactgaagcc tgatcatctg
    12061 gatctgccgg tagaggacaa gattacttat ttaatcaaga gcggcattcc ctggatcttc
    12121 tcccaggcca aagtggttga tgatgatttc agcgaacttc cccgggacgg caaatcagtc
    12181 ggcaatgtgg tggtccgcac tccctggtgc acccacgagt acttccatga gccggacaaa
    12241 acccgggaat tgtggaaaaa cgactggctc aacaccggcg acgtcggcta tatcgacccg
    12301 gaaggttacc tgatgatcac cgatcgcaac aaagatgcca tcaaatccgg tggtgaatgg
    12361 atttccaccc tgactctgga agatgccgtc agccagttca aaccggtgct ggaatgtgcc
    12421 gtaatcggca tccctcatcc ccgctatgat gaacggccct gcgcctgcat tgctctgaaa
    12481 ccgggtgaga ttgctaccgc agaagatgtc cgcgaccacc tgctgaaatt cgccgaggaa
    12541 ggcaagattg agaaatggtg gattccggat attccggacg gttacctgtt cctcgaggcc
    12601 atccctcaca attatatcgg caaaattgaa aaaaccacct tgagaaaaat gtatgccgag
    12661 atgaaaaaat aatctttcct ccaattgcca aagccccgga ctttcattgt attgtccggg
    12721 gctttggcgg ttcatgccga cgctcagccc tgctgcatct ggcaaaaccc tctatgccgc
    12781 ttgccgtgag caatgcacaa cgaaaagaac accctcatta ttagtttttt attaataaat
    12841 taaattttat tacaaatata tatatattat tccaacggat attatataat gttattagaa
    12901 attacactta ttaaataatg aagaggtgta aagtatgagt tatttga
//
LOCUS       DAOTHZ010000059        12697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5911_length_12697_cov_7.933950, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000059 DAOTHZ010000000
VERSION     DAOTHZ010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5911_length_12697_cov_7.933950"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..299
                     /gene="rpmG"
                     /locus_tag="P5549_07600"
     CDS             150..299
                     /gene="rpmG"
                     /locus_tag="P5549_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRW12974.1"
                     /translation="MRVGITLACTDCKQRNYQTTKDKKKNTEKMEVRKYCKFCNTHTI
                     HKEIK"
     gene            318..560
                     /gene="secE"
                     /locus_tag="P5549_07605"
     CDS             318..560
                     /gene="secE"
                     /locus_tag="P5549_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRW12975.1"
                     /translation="MAKNNTPAKQESGITIWWGNIREYLSSVYNELKKVYWPNRRQLI
                     AYTGVVIITVTIVALILWAFDSGLSFLLSKFMDAFA"
     gene            618..1193
                     /gene="nusG"
                     /locus_tag="P5549_07610"
     CDS             618..1193
                     /gene="nusG"
                     /locus_tag="P5549_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRW12976.1"
                     /translation="MPVETPAPAIADWLTERKGEWYVVHTYSGYENKIKVDLAKRVES
                     MNMQDKIFHVIIPEEQEVEFKGGKKKVTTKRVFPGYVIVNMILDDESWYVVHHTPGVT
                     GFVGSGTKPIPLQEEEITKILRRMGLVEQKPRIIDIEVGENVRVKNGPFENFEGIVKE
                     LLPDRGKIRVSISMFGRETPVELDYEQIDKI"
     gene            1206..1631
                     /gene="rplK"
                     /locus_tag="P5549_07615"
     CDS             1206..1631
                     /gene="rplK"
                     /locus_tag="P5549_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRW12977.1"
                     /translation="MAKRVMGKVSLQIKAGKATPAPPVGPALGQYGVNIMGFCKEYNA
                     KTANQTGFIVPVEITVYEDRSFTFVLKSPPASDLLKKAANIEKGSGEPNKKKVGTVNR
                     AKLVEIAEMKKDDLNAADLDAAVRMMEGTARSMGLKVVD"
     gene            1712..2416
                     /gene="rplA"
                     /locus_tag="P5549_07620"
     CDS             1712..2416
                     /gene="rplA"
                     /locus_tag="P5549_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRW12978.1"
                     /translation="MMNKSKAYSDAIKKIDKSKLYDPQEALALVKEIATAKFDETVEV
                     HIKLGVDPRHADQQVRGTVSLPNGTGKTRKVLVFAKGDKVKEAEQAGADYVGGEELAE
                     KIQGGWFDFDIAVATPDMMAVVGKLGKILGPRGLMPNPKSGTVTFDIERTINELKAGR
                     IEYRVDKTSIVHAPIGRVSFEVGKLQENLNAFAEALIKAKPAAAKGQYMRSVSICSTM
                     GPGIKINPLVIMAANK"
     misc_feature    2431..2578
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2637..3158
                     /gene="rplJ"
                     /locus_tag="P5549_07625"
     CDS             2637..3158
                     /gene="rplJ"
                     /locus_tag="P5549_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRW12979.1"
                     /translation="MPKIEEKQLIVDEIKQHLEKSMLVVFADYRGLKVDEMTELRNKL
                     RVPGVEVKVLKNTMLRFALEQTGNKDMADLINGPNAAILSQDDPVTPAKVLFEFAKTH
                     KNLEIKMGLLQGQVITPDKIKSLADLPSREVLVATVLGTMQAPITSFVRVLNANITGL
                     ARALDQIREQKAS"
     gene            3233..3610
                     /gene="rplL"
                     /locus_tag="P5549_07630"
     CDS             3233..3610
                     /gene="rplL"
                     /locus_tag="P5549_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRW12980.1"
                     /translation="MSKVQEVIDIVKGLTVLELSELVKALEEEFGVSAAAPVAAAAAP
                     TAAAAAPAEEEQTEFDVILASVGDKKINVIKVVREITGLGLKEAKDLVDAAPKPVKEK
                     INKEEANDIKAKLEEAGASIEIK"
     gene            4053..5357
                     /locus_tag="P5549_07635"
     CDS             4053..5357
                     /locus_tag="P5549_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12981.1"
                     /translation="MDWKEEYKRRLKTPEEAAKLIKNNDRIAMQGGTGIPPAFSAALA
                     KRSEELRNVVLCQGFALAFHDYMKPEYKDSFRVETVFIGPAERYCMSFGTVDFVPNHL
                     GSLGRWLDETKINKVAAVVTPPDENGFMNRSCFGGLCTKRLADRAENLIVEVNANTPW
                     LCSDVFQVHVSQCHAIIENNAPLFEIPEIPITPVEREIATIVADMIPDGSTIQLGLGG
                     LANCIGHFLKNKNDLGFHTEVVSNSIMELVKCGAANGSKKSIMKGKVPFTFCVGTKEL
                     WEYMDHNETFIGFEIENVNDPGIIRQNDNMVSVNNALMVDLTGQVAAESIGTYQYSAT
                     GGQMNFVLGSQMAKNGKSIIALPSTRTDKEGNRHSRILDMFPQGTTVSTSRNDVHFVV
                     TEFGAVNLRNRSISWRVANLINIAHPDFREELTFKAKQHGWL"
     gene            5417..6787
                     /locus_tag="P5549_07640"
     CDS             5417..6787
                     /locus_tag="P5549_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12982.1"
                     /translation="MRKTYLDEYKAKLITPEKAAQLVQANHVVDYGMFATKPIDFDIA
                     LSKRAGDGLKNVSVRGTGTVLPVPEVIKNDTEQKSFQYFSWYFTAIDRTAGDFGLACH
                     CPFNYHEATMMAYNPDFAAYWPDVWCAQVTPMDKAGCFNFGLGNSHNRGMALKCKIAI
                     VEVNENMPRCLGGSDEYVHINEIDYIIEGSNNPVFATPPSPEATPEERKIAQYIVEEI
                     VDGSCIQLGIGALPNLIGNMIADSDLKDLGIQSEMFCDSMVRMYETGRITNAKKARDI
                     NKSTFSFCLGTQDTYDFLDDNPQCASSAVYTTNSPEDVARNDNVVSINNILEIDLFTQ
                     VCSESSGIRQISGTGGQLDFVVGAFHSKGGKSFLAFSSTYKDKEGNIQSRVKPLLTPG
                     GIVTVPRTQVHYVVTEYGKVNMKAHSIWGRAEALINIAHPDFRDDLIKAAKEMKIWSR
                     TNQIPF"
     gene            6945..8318
                     /locus_tag="P5549_07645"
     CDS             6945..8318
                     /locus_tag="P5549_07645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW12983.1"
                     /translation="MTGLADLDKNIWGLLETIFAGFEGAIIIDEHGEIMLFSEYYAKE
                     MEIQREDVIGKNVLEVFPTTRLMEVIQTGQPIIADRWELLGKGHIVSRIPIEYQGKVI
                     GAIGVSVFPYLQMQYYINKLSTLSAELSYYKETVKRLSGAKYSMQSIIGQSEALLEAK
                     EKALKIADSTAPVILYGETGTGKELFAHAIHQESPRRNGPMIRVNCAGIPENLMESEF
                     FGYEEGAFTGAKKGGKPGKFELASKGSIFLDEISQLPYAMQSKLLRVLQEKEYERVGG
                     TEVLKADARVISASNVNLKKLLQQHLFREDLFYRLNVFVIRIPPLRERIGDIPLLAEH
                     FWQQQNSQNGTRIEGIATEAMRLLNNYAWPGNVRELETSIERACLDCQSGIIQIENLV
                     RFGGGKGSDKSNSPLPTLKESRESAEKAAILNALETAGGNKQKAADYLGIHRTSLYYK
                     MRELGLI"
     gene            8475..9134
                     /locus_tag="P5549_07650"
     CDS             8475..9134
                     /locus_tag="P5549_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12984.1"
                     /translation="MRKHIPIVFLSLFLCLLLCPVALAAVDWQIDWQENGAVQEKVLV
                     SGQELVHTETQWQKSSSDNQVTYSRSVENWQTYNNLADKIPVQAKVKDLFCCKIITLT
                     ALPEIQPNTLYAALKQNDVMRLQMNVPGFILDSSGTFGDNNIVGWEIDQPGQVFEQDF
                     FFRAIMIDGLGLGIVILSFGVIVLAIFFAVRMRKVNRLIDETYSLDNVVIDDEEEEQQ
                     S"
     gene            9388..>11015
                     /locus_tag="P5549_07655"
     CDS             9388..>11015
                     /locus_tag="P5549_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRW12985.1"
                     /translation="MAHMVKYGTVERLTYSQIVDVLELPNLIQVQLSSYDWFLTSGLR
                     TALQEVFPISDFTGHLELDFIDYSLGEPKYEVQECKERDVSYQAPLKLKVRLSDKESG
                     EIKESEVYMGDLPLMTDKGTFIINGAERVVVSQLVKSPGVYFNEKMDAAGNSLYGATI
                     IPNRGAWFELEMDSAGVIYTRIDKTRKIPVSVLLRALGYESNEDILGLFNNDEAIART
                     LDKDTTTNKKEALIEFYRRLRPGEIATEDAAEQLLKNLFFDPRRYDMAVVGRYKVNKK
                     LGLNTPVEIRNLTHEDIITTISYILKLINGEGKTDVIDHLGNRRLRSIGELLQNQFRT
                     GLVRMERVVRERMSIHDVETLTPQVLINIRPITAAVKEFFGSSQLSQFMDQTNPLAEL
                     TNKRRLSALGPGGLTRERAGFQVRDVHHSHYGRICPIETPEGPNIGLIGSLATYGRVN
                     DFGFIETPYRKVDKKKGQVLNEIHYLSADEEEEFIVAQANSPLDNKGKFKGERIEVRY
                     AEEILEVPINKVDYMDVSPKQVFSVATSLIPFLE"
     assembly_gap    11016..11115
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11116..12534
                     /locus_tag="P5549_07660"
     CDS             <11116..12534
                     /locus_tag="P5549_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRW12986.1"
                     /translation="TDNGELALGRNVMVAFMPWEGYNYEDAILLSEKLVKEDVFTSIH
                     IEEYECEARDTKLGPEEITRDIPNVSEEMLKNLDDQGVIRVGAEVRPDDILVGKVTPK
                     GETELTPEERLLRAIFGEKAREVRDSSLRVPHGEAGKIVAVKRFSRENGDELPPGVNR
                     LVRVYIAQKRKISEGDKMAGRHGNKGVISRILPEEDMPFLDDGTPVQIVLNPLGVPSR
                     MNIGQILECHMGWVANELGIKIATPIFDGATEEDIIELRTQAGLPPSGKAILRDGRTG
                     DRFDHEVTVGYVYMLKLAHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVW
                     ALEAYGAAYTLQEILTVKSDDVQGRLKTYESIVKGENIPEPGVPEGFKVLIKELQSLA
                     LDIKILSNDDHEIQIKDVDFALNEREKAIELGMDLPDLPMAHEGEGDKPAEDDFEPEE
                     DLDQVDLDEKEFSAPDLDDDLI"
BASE COUNT         3866 a         2436 c         3073 g         3222 t
ORIGIN      
        1 ggtggccgga cagtcggctc cggtgtagtt atcaacatcg agcaataaaa cattttcctc
       61 aaaacgtacc aaacatgctc tattgacatt gtttgcaggc atgtgctaga gtattgtagt
      121 atttttatac acgggatgga ggtgtcaagg tgagggtagg cattacgttg gcttgcactg
      181 actgtaaaca gcgcaattat caaacgacca aagacaagaa aaaaaacaca gaaaaaatgg
      241 aagtaaggaa atactgcaaa ttttgcaaca cccatacaat ccataaagag atcaagtaaa
      301 gctggggatg tgaaataatg gccaaaaata atacccctgc aaagcaagaa agtggtatta
      361 caatatggtg gggcaatatt cgcgaatatt tgtccagtgt ttataatgaa ctcaagaaag
      421 tatactggcc caaccgcagg caattgattg cttataccgg tgttgtaatc ataactgtga
      481 caattgttgc gctgatactg tgggcttttg atagtggctt gtctttcttg ttgtcaaaat
      541 ttatggatgc ctttgcctaa aggaggtggt tgccaatgac cgccaaaaag caatccaaat
      601 caaaggagaa cgctgtcatg cctgttgaaa caccagcccc ggctattgcg gattggctga
      661 ctgaaagaaa aggcgaatgg tatgtggtgc atacctattc cggttatgag aacaaaataa
      721 aagtagattt ggccaaacgg gttgaatcca tgaacatgca ggacaagatt ttccatgtaa
      781 ttattcctga ggaacaggaa gttgaattta aaggcggcaa aaagaaagta actaccaaac
      841 gggtattccc gggatatgtt atcgtcaata tgattttgga tgatgagtct tggtatgtcg
      901 tgcatcatac cccaggtgtt accggtttcg tgggcagtgg taccaaacca atccctttgc
      961 aggaagaaga aattaccaaa atattgcgtc gaatgggttt ggtcgaacaa aaacccagga
     1021 taatagacat cgaagtggga gaaaatgttc gcgttaaaaa cggaccattt gaaaacttcg
     1081 agggaattgt caaggaattg ctgccagacc gcggcaagat aagagtcagt atttccatgt
     1141 tcgggcggga gactcccgta gaattggatt acgaacaaat cgataaaata taaggaggtg
     1201 tgaatgtggc caaaagggtt atgggcaaag tttctctgca aatcaaagca ggcaaagcga
     1261 caccagcacc cccggtagga cctgcgttgg gtcagtatgg tgtcaacatc atgggttttt
     1321 gtaaagaata taatgcaaaa acagccaacc agaccggatt catcgtgccg gtagaaataa
     1381 ccgtttacga agacagatct tttacctttg tgctgaaatc tccgccggca tctgacttgc
     1441 tcaagaaagc tgccaatatt gaaaaaggtt ccggagaacc caacaagaaa aaggtgggta
     1501 ctgtgaatcg agccaaactg gttgaaattg ccgagatgaa aaaagatgat ctcaatgctg
     1561 ccgatttgga tgctgccgtg agaatgatgg aaggaaccgc acgcagtatg ggactcaaag
     1621 tagttgatta atctgcagtt ggaattacag tattttgaga tacggttgaa caatgtggga
     1681 ggagcattcc gcaaaccaca aggaggatta aataatgaac aaaagcaaag cttattccga
     1741 tgcaatcaaa aaaattgaca aaagcaaact atatgatccg caggaagcgc ttgcgctggt
     1801 caaggaaatc gcgacagcca agtttgatga aacggttgaa gttcacatca aattgggcgt
     1861 agatccccga catgccgacc aacaagtcag agggacagta tctctgccca atggaaccgg
     1921 caagactcgt aaagtactag tgtttgccaa aggcgataaa gttaaggaag cagagcaggc
     1981 tggtgcagat tatgtcggtg gagaagaact ggcggaaaag attcaaggcg gctggttcga
     2041 ttttgacatt gcggtggcaa cacctgatat gatggcggtt gtcggaaaac tgggtaaaat
     2101 attggggccc cgcggattaa tgcccaaccc caaatcaggt acggttactt tcgatataga
     2161 gcgtaccatc aacgaattga aagccgggcg tattgaatac agggttgata aaacctcgat
     2221 cgtgcatgcc ccgattggca gagtatcttt tgaagtggga aaacttcagg aaaatctaaa
     2281 tgcttttgcc gaagcattga ttaaagccaa accggcagcg gccaaaggtc aatacatgcg
     2341 ttccgttagt atctgctcaa ccatgggacc cggtattaaa atcaatccgc ttgtaattat
     2401 ggccgcaaac aagtaaaatg atggaaatct aaaactgaaa attgataact gtagatagct
     2461 ggtggcgcaa gccttaaaaa agttttaaat ttgcctgctg aggttagtga aatacatgtt
     2521 ctataatgga cgctattaac ctctgcaagc ctgattgcag aggtttttcg atttctctga
     2581 ccattgccaa cgaaaccatt taaatcgaat acatcatcat aagggggtga aaatgaatgc
     2641 ccaaaatcga agaaaaacaa ctaatcgtgg atgaaatcaa gcaacacctg gagaaatcaa
     2701 tgctggtggt ttttgctgat tatcgtggct taaaagttga cgagatgacc gaactgagaa
     2761 acaagttacg cgttcccggc gttgaagtca aagttctgaa aaacaccatg ctgcgttttg
     2821 cgctggaaca aaccggcaat aaagacatgg ctgatttgat caacggtccc aatgctgcca
     2881 tcctcagtca ggatgatccc gtcactccag ccaaggtatt gttcgaattt gccaagacgc
     2941 ataaaaatct ggaaatcaag atgggacttt tacaaggaca agtgataacg ccagataaga
     3001 ttaaaagtct ggccgatctg ccttcgcggg aagtattggt ggcaactgtt cttggaacca
     3061 tgcaggcgcc catcacatca tttgtgcgcg tgttaaatgc caacattaca ggtttggcaa
     3121 gggcactgga tcagattcga gaacaaaaag ccagttagta atttgggaat aaacacatct
     3181 gcattccctt acaaaaacat catttaagcg aaattgaagg aggatattta aaatgagtaa
     3241 agttcaagaa gttattgata ttgtaaaagg tctgacggtt ttggaactgt ccgagctggt
     3301 taaagccctg gaagaagagt tcggtgtaag tgctgccgct cctgtagccg cagctgcagc
     3361 cccgaccgcc gctgctgctg ctcctgcgga agaagaacag actgagtttg atgtaattct
     3421 ggcttctgtt ggcgacaaga aaatcaatgt catcaaggta gtcagagaga tcaccggatt
     3481 agggctgaag gaagccaaag atttggtaga cgccgcacct aagccggtaa aagaaaaaat
     3541 caacaaagaa gaagccaacg atatcaaagc caaacttgaa gaagctggtg ccagtataga
     3601 aattaaataa tgtttacgaa atgattggag aacccgctta gggttctctt ttttattgga
     3661 atagacgaga tttaaagcac cacccttgga tggggaggtg cttttttggg gtgtaaatgt
     3721 ggcagataga agggaacaaa gactgccttc tacccaaggg gactgaaagg tgtagcaaaa
     3781 accaacatat gtataaaata gtctacacgt atcgcagaac cctgacaggt atgcattatt
     3841 atcgataaaa aaaacaatag actatataaa taacgcaaca ttcaaaaaat aaaaaatatt
     3901 caaacaaaac aagaagcttg tgattgccat gttgtgtgga tgtgaaaaaa tggtacacat
     3961 attgcaaatg taatatagct actgcatcat tacaatcatt ttacacagca atataatctt
     4021 tatccaccac aaataacgaa ggagggtata aattggattg gaaagaggaa tacaaacgca
     4081 ggcttaaaac tccggaagag gcagccaagc ttatcaaaaa caatgatcgg attgccatgc
     4141 agggggggac cgggatacct cccgcatttt cagccgcatt ggccaaaagg tcagaggaat
     4201 tgcgaaatgt agtcttgtgt caaggatttg ccttggcttt tcatgattat atgaagcccg
     4261 aatacaagga tagttttcgg gttgaaacag tatttatcgg gccggctgaa cggtattgca
     4321 tgagttttgg aaccgttgat tttgtcccca atcacttggg cagcctgggg cgttggctgg
     4381 atgagaccaa aatcaacaag gtagcggctg tagtcacgcc gcccgatgaa aatggattta
     4441 tgaatcgttc ctgctttggc ggattgtgta ccaaacgatt agccgaccgg gcagaaaatt
     4501 taatcgttga agtcaatgcg aataccccct ggttgtgcag cgatgttttc caggttcatg
     4561 tttcccaatg ccacgcaatt attgagaata atgccccatt gtttgagatt ccggaaatcc
     4621 ccatcacgcc ggttgaaaga gaaatcgcga cgattgttgc agacatgatt ccggatgggt
     4681 caaccattca gttgggactg ggcggattag ccaactgtat tggtcacttt ctgaagaata
     4741 aaaatgattt gggcttccat accgaagttg tttccaactc gattatggaa ctggtcaaat
     4801 gcggtgcagc caacggcagt aaaaaaagca taatgaaagg caaggtgcct tttacgtttt
     4861 gtgtgggaac caaagaacta tgggaataca tggatcacaa tgaaaccttc ataggttttg
     4921 agattgaaaa tgtcaatgat cccgggatca tccgccagaa tgacaacatg gtctccgtca
     4981 acaatgcgtt gatggttgat ctgactggac aggtagccgc tgaatcgatc ggtacatatc
     5041 agtacagtgc taccggaggg caaatgaatt ttgttttggg ttcccaaatg gctaaaaacg
     5101 gaaaaagcat cattgccctg ccatcaaccc gtaccgataa agaaggcaac cgtcattccc
     5161 gcattttgga tatgttcccg cagggaacga cagtttccac ttcccgcaat gatgtccact
     5221 ttgtggttac tgagtttggt gcagtcaatt tacgcaaccg gtcaatctct tggagagttg
     5281 caaacctgat taatattgct catcctgatt tccgagaaga actcacattt aaagccaaac
     5341 aacatggatg gctttaagtt ctgaaatttt acagcagccc ggttatgcca aatagaaaag
     5401 tgaaggagga tgaacaatga gaaagactta tctggatgaa tacaaggcca aattaatcac
     5461 cccggaaaaa gcagcccaat tggttcaagc caaccatgtc gttgattacg gaatgtttgc
     5521 caccaagccg attgactttg atattgccct atcaaaacga gccggtgacg ggctgaaaaa
     5581 tgtaagtgtt cgaggcacgg ggacagtcct gccggttccg gaagtcatta aaaatgatac
     5641 cgaacagaag tcatttcagt attttagctg gtattttacg gcgattgatc gtacggctgg
     5701 ggactttgga ctggcctgtc actgtccttt caattatcat gaggcgacca tgatggctta
     5761 taatcctgat tttgcagcat actggccgga tgtttggtgt gcccaggtta cacccatgga
     5821 taaagccggc tgttttaatt ttggactggg taattcccat aaccgtggga tggcccttaa
     5881 atgtaaaatt gccatcgtgg aagtaaatga gaacatgcct cgttgtctgg gtggaagcga
     5941 tgaatatgtt cacatcaatg aaattgatta tattattgaa ggttccaaca accctgtttt
     6001 tgcaacacct ccttcgccgg aagcaacccc ggaagaacga aaaattgccc aatacattgt
     6061 tgaggaaatt gttgatggtt cctgtataca gttgggcatc ggcgcacttc ccaatctcat
     6121 cggcaatatg atcgccgatt ccgatttgaa ggatttgggc atacaaagcg aaatgttctg
     6181 cgattcgatg gtaaggatgt atgaaaccgg aagaatcacc aatgccaaaa aagcaagaga
     6241 catcaacaag agcacttttt ctttttgtct gggcacgcaa gatacctatg attttttaga
     6301 tgacaatccc cagtgtgctt cctcggcagt ttataccacc aacagccccg aggatgttgc
     6361 cagaaatgat aatgttgtat ccatcaacaa tattttggaa atcgatttgt ttacccaggt
     6421 atgttcagaa tcatccggga taagacaaat atcaggcacc ggaggtcaac tggattttgt
     6481 cgttggggcc ttccattcca agggcggaaa aagttttctg gctttttctt ctacctataa
     6541 agacaaagaa ggcaatattc aatcccgggt caaaccattg ctgaccccgg gcgggatcgt
     6601 tactgttcca cgtactcagg ttcattatgt ggtgactgaa tatggcaaag tgaacatgaa
     6661 agcccattcc atttggggaa gggcggaagc tttgatcaac attgcccatc ctgattttcg
     6721 cgatgatttg atcaaagctg ccaaggagat gaaaatctgg tcacgaacca atcagattcc
     6781 tttttaatcg atcctcccat gtgcagttaa acaaataaat aaaaaaacga gtgcttaccg
     6841 ttcccgattg ccatatacta gtaattagta tatggcaatt taaatacgga tcgtcatctc
     6901 agcgtctgat tgatcatagc aggaagagcg gggaggtaag cctcttgacg ggactggctg
     6961 atttggacaa aaacatttgg gggctgctgg aaactatttt tgccggcttt gaaggtgcca
     7021 ttattattga cgagcacggt gaaattatgc ttttcagtga gtattatgcc aaagaaatgg
     7081 aaattcaacg cgaagatgtg atcggtaaaa acgttctgga ggtatttccc accactcgat
     7141 taatggaagt catccagacc ggtcaaccga ttattgccga tcgttgggag ctattaggca
     7201 aaggccacat tgtatcccgt attcccatcg agtatcaggg caaggtcatc ggtgcaattg
     7261 gtgttagtgt atttccatat cttcaaatgc agtattatat taacaagctt tcaacactta
     7321 gtgcggaact ttcatattac aaggagacgg ttaaaagact gagcggtgcc aaatactcca
     7381 tgcagtccat cattgggcaa agcgaggcat tgctggaagc caaagaaaaa gcacttaaga
     7441 ttgccgattc cactgctccg gtaatacttt acggcgagac gggtaccggt aaagagttgt
     7501 ttgcccatgc cattcaccaa gaaagcccgc ggcgaaacgg gcccatgatt agggttaatt
     7561 gtgccggcat acccgaaaac ctaatggaat cagagttttt tggttatgaa gagggagcct
     7621 ttaccggtgc taaaaaagga ggcaagccgg gtaaattcga acttgccagt aaaggcagta
     7681 tttttctgga tgaaatcagc cagcttccgt atgccatgca atcgaaatta ttaagagtgt
     7741 tgcaggaaaa agaatatgaa agggtaggcg gaacagaagt gttaaaagct gacgcccggg
     7801 tcatttctgc ttccaatgtc aatcttaaaa aattattgca acagcatctc ttccgtgaag
     7861 acttgtttta ccgattgaac gtatttgtaa ttcgtattcc tcctctgcgg gaaagaatcg
     7921 gtgacattcc acttctcgcg gaacattttt ggcaacaaca gaacagccag aacggtaccc
     7981 gaattgaagg catagcgacc gaggccatgc gccttttgaa taactatgcc tggccgggga
     8041 atgtaagaga actcgaaacc agtatcgaga gagcctgttt ggattgccag agcggaatca
     8101 tccagattga aaatctggtc cgttttggtg gagggaaagg ttcagacaag agtaattctc
     8161 cactgcctac tttaaaggaa tcaagggagt cggctgaaaa agctgccatc ctcaatgcct
     8221 tggaaacagc tggcggaaat aaacagaagg cggctgatta tctagggatt catcgtactt
     8281 ctttgtatta taaaatgcgt gaattaggcc ttatttagca tttctaaaag gaaacttcat
     8341 ttttgcaagc actgatcctt caaattaaca aaatatccgt tattagaatt tatgaaataa
     8401 attaaatcat ggtctatctt ggattaatgt tataaaatta ggtatagatt cagaaatgag
     8461 gggaatgata aaaaatgagg aaacatatcc cgatcgtttt tttaagcctt tttttgtgtc
     8521 tgttattatg tccggttgcc ttggctgcgg ttgattggca aattgattgg caggaaaatg
     8581 gtgcggtcca ggaaaaagtt ttggttagtg gccaagaatt agttcacaca gaaacgcagt
     8641 ggcaaaagag cagttccgat aatcaagtga cttacagccg tagcgtagaa aactggcaaa
     8701 catacaataa ccttgccgac aaaattccag tacaggccaa ggtcaaagat ttgttttgct
     8761 gtaagattat taccctgact gcactgccgg agatccaacc caatacgctt tacgcggcgt
     8821 tgaagcaaaa tgatgtaatg cgactgcaaa tgaatgttcc cggatttatt cttgatagta
     8881 gtggtacttt cggggacaat aatatagttg gctgggaaat cgatcaaccg ggacaggttt
     8941 tcgaacagga tttcttcttt cgggcgatta tgattgacgg gctggggtta ggcattgtaa
     9001 tcctttcttt tggggttatc gttttggcaa tcttctttgc ggtacggatg cgcaaggtca
     9061 atcgactcat tgatgagacg tattcattgg ataatgtcgt catcgatgat gaagaggaag
     9121 agcaacaaag ttaggaaaaa attggggata attttaataa aaagattgtt cgatacataa
     9181 taattctatg ttataaaaat attaataatc attccttgac tggaaaaatg ttatgtgata
     9241 acattaataa ttgccattat agatgtataa tggtatcgtt atgcaaaggg tcaagttatt
     9301 ttatgctttg aaaaaataaa agcaaggttt attagagtta ggccttgctt ttcactcttt
     9361 tgccaacttt tataaggggt gaagtgaatg gcacacatgg tgaaatatgg tactgtcgag
     9421 aggctcacat attcccagat cgtcgatgta ctcgaacttc ccaatctcat tcaggttcaa
     9481 ttaagctcct acgattggtt tcttacctca ggcttaagaa ccgcccttca ggaagtattc
     9541 cctatctcag actttaccgg tcatcttgaa ttggatttta ttgattacag tcttggtgaa
     9601 ccgaagtatg aggtacagga gtgcaaggaa agggatgtca gctatcaggc tcccttaaaa
     9661 ttgaaggtac gactcagcga caaggaaagc ggagaaataa aagagtcgga agtctacatg
     9721 ggagatctgc cgttgatgac cgacaaagga acattcatta tcaacggtgc agaaagagtt
     9781 gtggtaagtc agctggtcaa atcgccgggc gtttatttta atgagaaaat ggatgcagcc
     9841 ggtaacagtc tgtatggagc aacgattatt cccaatcgcg gggcatggtt cgaactggaa
     9901 atggacagtg cgggagtgat atacacccgt attgacaaaa ccagaaaaat tcccgtatcc
     9961 gttttgttac gtgcgctggg ctatgagagt aatgaagata tccttggttt gttcaacaat
    10021 gatgaggcga ttgcccgtac cttggacaag gataccacga ccaataaaaa ggaagccttg
    10081 attgagtttt atcgtcgttt gcgtccgggt gaaattgcga ctgaggatgc tgccgaacaa
    10141 ttattaaaaa atctcttttt tgatccccgc cgttatgata tggcagtagt cggcagatat
    10201 aaagtcaata aaaaactggg attaaatact ccggtggaga ttcgcaacct gacccatgaa
    10261 gatatcatta ccaccatcag ctatatattg aagctgatta atggcgaagg caagacggat
    10321 gtcattgacc atttgggcaa ccgtcggtta cgctcgatcg gtgaattact acagaatcaa
    10381 ttccgtaccg gtttagtcag gatggagagg gttgtgcggg aaaggatgag tatccacgat
    10441 gtggagaccc tcacgccgca ggtgttaatc aacatccgcc ccattacggc tgcggtaaaa
    10501 gaattttttg gcagttccca attgtctcag tttatggatc agaccaatcc gttggcggag
    10561 ctcaccaata aaagaaggtt aagtgctttg gggccagggg ggctgacacg tgaacgtgca
    10621 ggtttccagg ttcgtgacgt tcatcactcc cattatggga ggatttgtcc gattgagacc
    10681 cctgaaggac cgaacatcgg cttaataggc tcgctggcaa catatggccg ggttaatgat
    10741 tttggtttta ttgaaacccc ctaccgtaaa gtcgacaaga aaaaaggtca ggtgttaaat
    10801 gagattcact atctgtcggc cgatgaagag gaggaattta ttgttgccca agccaattca
    10861 ccgttggaca acaaaggtaa attcaaaggg gaaagaatag aagtaaggta tgcagaagaa
    10921 attcttgaag taccgatcaa caaagtagat tatatggacg tatctcccaa acaggtattc
    10981 agtgtggcaa cttcgttgat tccattcctg gaaaannnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnacaga taacggagaa ctggctttgg gccgcaatgt tatggttgcg
    11161 tttatgcctt gggaaggata taactacgaa gacgccatcc tgctgtccga gaaactggta
    11221 aaggaagatg tattcacctc gattcatatt gaagaatatg agtgtgaagc ccgtgatacc
    11281 aaactgggac cggaagagat tacccgtgat attcccaatg tatccgagga aatgttgaaa
    11341 aaccttgacg atcaaggagt aatcagggtt ggagcagaag tccggccgga tgatattctg
    11401 gttggcaagg tcacacccaa aggagaaacc gaactgacgc cggaagaaag actgttacgc
    11461 gccatatttg gcgaaaaagc ccgcgaagtg agggactcat ccttacgggt accgcatggt
    11521 gaggcaggca agattgttgc cgtaaaacgg ttctcccgtg aaaacggtga cgaattgcca
    11581 cccggtgtaa atcgactggt gcgggtatat atagcccaga agaggaaaat ctccgaggga
    11641 gataaaatgg ccggacggca tggcaacaaa ggcgtcattt caagaatctt gccagaagaa
    11701 gacatgcctt tcctggatga cggtactccg gtacaaattg ttctgaatcc cctgggcgta
    11761 ccgtcccgta tgaatatcgg tcagattctc gagtgtcata tgggttgggt tgctaatgaa
    11821 ttgggaataa aaattgcgac tcccatattt gatggggcta cagaagaaga tattatagag
    11881 ctgcgcacac aggctggtct cccgcccagc ggcaaagcaa ttctgcgcga cggcagaacc
    11941 ggggacagat ttgaccatga ggtaacagtt ggttatgtgt atatgctgaa gttggctcat
    12001 ttggtcgatg acaagatcca tgcccgatcg attggtccat attcactggt tacccaacag
    12061 ccgctgggag gaaaagcaca gtttggcgga cagcgcttcg gtgaaatgga agtatgggct
    12121 ttggaggcat acggtgcggc ctatacatta caggaaatac tgacggtgaa atcagatgat
    12181 gtccagggac gattaaaaac ctacgagtca attgttaaag gagagaacat ccctgaaccg
    12241 ggagtgccgg aaggatttaa agttttaata aaggaattgc aaagtttagc cctcgatatc
    12301 aaaatactgt ccaatgatga tcatgagatt cagatcaaag atgttgattt tgctcttaac
    12361 gaaagagaaa aggcgatcga attgggaatg gatctgccgg atttgccgat ggctcatgaa
    12421 ggtgagggtg acaagcctgc agaggatgat tttgaaccag aggaagatct tgaccaagtt
    12481 gatctggatg aaaaggagtt ttcagccccg gatctggatg acgatttaat ataaggcttg
    12541 tatcaacccg tatcatagca tctaaatgac caagtaagca cttcttctgg aagggagaga
    12601 atgatttgtt agatgtcaat aattttgaac ggatcaaaat atccctggct tctcctgaac
    12661 aaattcgttc ctggagcagc ggtgaggtaa aaaaacc
//
LOCUS       DAOTHZ010000060        12694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5912_length_12694_cov_6.513727, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000060 DAOTHZ010000000
VERSION     DAOTHZ010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12694
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5912_length_12694_cov_6.513727"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="P5549_07665"
     CDS             <1..260
                     /locus_tag="P5549_07665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRW12987.1"
                     /translation="VGGLSEIVEHRQTGLKVPPGHESKLAEAIIELLTNTEYARQFAK
                     NAAQAIASTYSWETIAHSTIAVYEEVIARMGVKTSGHSDVG"
     gene            283..1842
                     /locus_tag="P5549_07670"
     CDS             283..1842
                     /locus_tag="P5549_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRW12988.1"
                     /translation="MESNIMLVLHSHIPFVKRQGRWPFGEVWLFEAMAETYIPFVRTW
                     LRLREEGIKAPITLSLSPPLLDQLSSPYIQKEFVAYLREREDKAAEEERYYMSVAEPN
                     MASLASFYHRFYRDIRRDFTLELDSNLVNVFKQLKDNGEVEIMATAASHAYLPLLDRK
                     SLQHQILWGIRAYENYFACEPEGFWLPECGYFQGIEDLLIARQLRYFYVDSHALEGGK
                     PLGDSSIVDPELTCQDEMFANTGLSTYRPYKIKDKDILVFGRNAMVSQQVWSADYGYP
                     GDKDYREFHRESPRSGFKYWRITDRNNGIKLVYEPEQARLKARQHAHHFINSIRNTGR
                     EAAHLGYDQPLIIACYDTELFGHWWWEGIDWLEEVVRGIAHSQELDFILPAQIHFPDG
                     EAQVFESSWGTGGKHYIWKNKETQWMWDIIRQASNEFEELNKSSIDSDVKAASLAQAL
                     RELILIESSDWLFMVTNNLTRDYAMKRFFEHYTKFLRLVNAIRDENFSENYVDWLMNV
                     EVEDDFLSFLK"
     gene            1863..4349
                     /locus_tag="P5549_07675"
     CDS             1863..4349
                     /locus_tag="P5549_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12989.1"
                     /translation="MKGYIAFCPHFHQPHFQLYKTREEAFANSYQPWLELLQEAAGLD
                     KFYINLHFSGPFLYWFRQQKPAYLRDFKNLLNTQKIGLIGGLADEPFIQLSSRIDDCL
                     YQIKKYDELCRELFGVEARDWQGIHLVERECGEYLLRHLTQAAYLLGAPPLYYLDAET
                     FYQPHFSRPGGLYDYCLKHFGFKDPVALTTIAHIPDEMLYFGLQDEIGGQIFHAIPVH
                     TQFRYQLLKRDAFTADDRVQIKPSHYYFYLKDTLEQAHKFAGQYGRALDPILVIFEDA
                     EKMGQWSKDPQGDKQWLMEFFRLVEADPDVEFIGLQDYINRVGILDTYPVSSSHSYPE
                     WENWTAKRGIRGVTYGDERLRRVISRLRLFEKQQDAFEHHIVGNALKITASAELKDMQ
                     IRAIMNSLERYVFIDQYLAEAYPPHYRDYYQIINRVRNLVYQEDPKWASRHPSYGSSP
                     FYDMQGLAYLEIAHRLLHQLMQEMGCETTENKSANLLDWDFDGQTEVVLASPAQTLAI
                     DPVGGCIQYHFVLRDDLTNFAALANLLQQNFSGLDAYHSVYRYAYPLIMTETDSSMKA
                     EIFPEGARREICRNSLRCSLLIENNGVFTEIGDFDRALYELEGVFREDNCCRALLSCT
                     KSINLNHQAGIVKLSKEYLLQDNGLSLTIKAAFEEISPGNCFLAVQVVSSAAPSDEID
                     FHPLAWLELPAGEGKPNVDVQQAVQWNNGEVEFVNKTCSAGSLDQIDYVFLIHTGQGD
                     LFGNRICYHFTGDEIEKLEVRPAVENYYQGLVFDHQSKLGYQSSGLMLIPYIPVKNGV
                     AAFTVDMHWQLDDDYERVSNRQRLYLIDRF"
     gene            4374..5579
                     /locus_tag="P5549_07680"
     CDS             4374..5579
                     /locus_tag="P5549_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRW12990.1"
                     /translation="MLRKFFDYYFERVDQDVLQLYQQLMPDCKDLAESYAAIFTRNDE
                     ESINFLLDFYRATADLRHKIPQQSILFNTHDNICFPFFVEQVWNMLDEQGRAAVAARV
                     GNDDINAIVAAFTSDVALRNWFRQEIAVNLGQNDLFSLNEVIALQNFSSGGESSFLKY
                     VYPHVQKVNGRVLDAGCGAGFATMVMSQFADVYSIDACQPRLERATALSNMMRKGEKE
                     FFSGVFALIDEELGDLAENVHLNVSAELLNGKPRQVEFTVGSIDALPYPDQYFAMINC
                     LDVLEHTYDPAKIIQQFARVLQPGGRLFITVPTKYGEVVQHYYERLEGSMFPAMLHMH
                     HFDPATLNGIFKQNGFKEFQLTPFDYMDWPSFVEIADQLPQTKLVADLKSQPAETVAL
                     QLFAIYEKI"
     gene            complement(5580..6125)
                     /locus_tag="P5549_07685"
     CDS             complement(5580..6125)
                     /locus_tag="P5549_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW12991.1"
                     /translation="MVRNYRILMIPLIAVILFLTFYFIYSGQKQSTPPPSRKPAPGQQ
                     SEKPPGKWYVEFSVKQQKTTAYTEAANAPVSSAGRSYFIGSGAVHPRYPLQSGGEARS
                     PIIPFGTILYLQEPVTIQGQTHTELTVNDTGDVYYGLWPAHPYWVDVYFGTTNYYNQQ
                     SASKAGVQMIDYKWYEPWPEK"
     gene            6249..6683
                     /locus_tag="P5549_07690"
     CDS             6249..6683
                     /locus_tag="P5549_07690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRW12992.1"
                     /translation="MPIVNAGISPQLFDYLVKSMTDSPFYKLMEIKITLLGEGQAELE
                     VQAQEKHTNPIGLVHGGLIMSMADAAMGNAIRSIGVIGVTVDCSVSFPGSARLGEKLL
                     ARGKVIKAGHKMIFAEATVWAGERILGHSKSTFFNTGKIELT"
     gene            6693..6992
                     /locus_tag="P5549_07695"
     CDS             6693..6992
                     /locus_tag="P5549_07695"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRW12993.1"
                     /translation="MSDHLLNLQEFYLFTFVSTSHALQGERILKAQQAEFIMIPTLRE
                     ISASCGLSIKIRPENLAQYRQIFLDHRVKAENYYHVRKDGKQYMIKAFPFNTKEQ"
     gene            7041..9131
                     /locus_tag="P5549_07700"
     CDS             7041..9131
                     /locus_tag="P5549_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRW12994.1"
                     /translation="MKNPDQSAMENQIELLLGNAGILSQHLPAFRFRSQQKLLAENIA
                     AALADDGFLLAEAGTGIGKTFAYLIPAITWCQQTKNKVVVSTRTKALQQQITEDDLPA
                     LLRIMNSRIKTAEAKGRDNYLCWNKYQQILAGKKALSAAEQDFVQAILVWAERTKSGD
                     RKELELPSEMMQSWPVLAADRTSCLRDQCRYQDKCFRQKMIRNVHKADLIIVNHALLL
                     SDILIENREKSILPDYEYLIIDEAHTFIKESFEQLSYRFSSYENKRVLGALHEKARGK
                     VRRGYLLHLLSAHPHLSALITEATTYADQSQRWIDEFFNSLNDIAVYSDNYAYHHVIK
                     PGELNQPALQRADRAFREWRREIRLLIDKLTETRDEIASEDDFLELSGLINALTELGI
                     VASIIMEDNLLDPQAICWLEYEKGRVSSICSSPIYTGDSLDEKLYRKLNGLVMLSATM
                     TVDNNFDNYINRSGLRWYEQDGRLNTMIETSPFDYENNAALYIVEDMPDPGNTRFFTE
                     LQRVLTEIVLLSRGRTMVLFTARKQMEETAACLRPLLEANDIKLLVQYQDGEFASLMD
                     GFKSHANAVLMGLETFWEGIDLKGEVLKCLVIVKLPFRSPSDPYCTAWEKHYLQQRMN
                     SFEHFMLPDATIRLKQGVGRLIRSETDRGLVLVLDTRLIYKKYGQLMQNSLPLTNINA
                     VSSAKLAEIIKPWF"
     gene            complement(9509..9880)
                     /locus_tag="P5549_07705"
     CDS             complement(9509..9880)
                     /locus_tag="P5549_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HRW12995.1"
                     /translation="MNIGKSAELQGLLRSLLLGIVACLAAALVLYFSALEETSAGWIA
                     QLIVIGSVFYAGCHVSKSYGNKGLIRGLTMGIIYYIILLIASLVFQTGGIDIQNYLIN
                     LLICLAAGGFGGILGIGLNAE"
     gene            9997..10701
                     /locus_tag="P5549_07710"
     CDS             9997..10701
                     /locus_tag="P5549_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRW12996.1"
                     /translation="MNNALWWSFLAGFSTSIGAALLFLNKKWRPRHLAYFLGLAAGVM
                     TAVVFFDMLPSALVSGSSKALSGVVIGLLLMFLTAGIQHQGIHSTQTLVGLGYMIMLG
                     IALHDLPEGMAVALGEHMKNRTGMVIALGIGIHNMPEGMAIAAPLLMSGMKKSMVLLQ
                     TLLISLITPLGTLLGSFLLHLAPGLMPLMLGIASGVMLYLVIFQLWPQAGYKNKSSRW
                     PGFCLGMLVIGLATYL"
     gene            10886..12589
                     /locus_tag="P5549_07715"
     CDS             10886..12589
                     /locus_tag="P5549_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12997.1"
                     /translation="MVLFNPHNYNKEHLDPRAREIMLKTIDFFEDKGLASIKEDDKNF
                     TWYQDYVDFIGKEEIFADLLTPAGYGKEDSRWDLWRVAEFNEVSGFYALHFQYLYQVT
                     ILGLGPIFLGNNEAVKHKAAQLLREGGIFGFGLSERDHGNDIYSDEMTLYPNGDGTYR
                     AKGNKYYIGNGNKAALLSVFGKFADTGEYVFFAVDTKHPNYELVKKINTNGAHCAYVA
                     EFNLNDYPVTDADILSRGPHAWDSALNTVNIGKCMVGWASIGEATHALYESLHHTCNR
                     QLYGKPVYAFPHVRRLFTESYLRLVTMKLYALRCMDYFRSASDQDRRYLMYNAILKMK
                     VCSQGDKISQMLLDIVGAKGMEQDTFMELFIRDAGMMPRLEGTTHVNMALVNKFFKPY
                     FFGPVDFPHIPKRNDIANDNYVYKQKAGGLKEVRFGDYSLCYEGFNQANVLKFREQVE
                     LLKHFLANNTPSPEQAANVDYMIALGELLAATAYAQLTLENAKIYGIEDDVLEEIFAF
                     MIRDFAAYALMFRSNFVQGPDQLETINKMIIDPVQDPARFDKIWEERVLSLKDAYVMN
                     D"
BASE COUNT         3366 a         2951 c         3087 g         3290 t
ORIGIN      
        1 aagtaggggg gctatcggaa atcgtggagc atcggcagac gggtttgaag gttccgcccg
       61 ggcatgaatc caagctggcg gaagccatca ttgaattatt gacgaacacc gaatacgccc
      121 ggcaatttgc gaaaaacgcc gcacaagcca ttgcctcaac gtatagctgg gagaccatcg
      181 cccatagtac cattgcggtt tacgaagaag tcatcgcccg tatgggggtt aaaacatccg
      241 gccacagcga tgtcggttaa tacttttaag gaagtgtagc cattggaatc caatatcatg
      301 ctggtgctgc acagtcacat cccattcgtt aaaagacagg ggagatggcc ttttggtgaa
      361 gtttggctat ttgaagccat ggcggaaacg tatatcccct tcgtacgcac ttggctgcgc
      421 ttgcgcgaag aaggtatcaa agcaccgatc accttgagtc taagtccgcc cttgttggac
      481 caactgagtt caccatatat acaaaaagaa ttcgttgcct atctgcgcga acgtgaagat
      541 aaggctgcgg aagaggaacg ttattatatg tcggtcgctg agcccaatat ggcttcgctg
      601 gcctcttttt atcatcgctt ttaccgggat atcagacggg acttcaccct tgaactggac
      661 agcaacctgg tcaatgtgtt caagcaattg aaagacaacg gagaagttga aatcatggcc
      721 actgctgcca gccatgccta tctgccactg cttgaccgca aaagcctgca acatcaaatc
      781 ttgtggggca tccgcgctta tgaaaactat tttgcttgtg aaccggaagg gttttggttg
      841 ccggaatgcg gatatttcca aggcattgag gatcttttaa tcgctcgtca actgcgctat
      901 ttttatgttg acagtcacgc cttggaaggc ggaaagccac tgggggactc cagtatcgtc
      961 gatccggaat tgacttgtca ggacgagatg tttgccaata caggactcag cacctatcga
     1021 ccctataaaa tcaaggacaa ggacattttg gtttttggcc gcaatgccat ggtcagccag
     1081 caggtctggt cggctgatta cggttatccg ggggacaagg attaccgcga attccatcgg
     1141 gagtccccac gttctggttt taagtattgg cggatcaccg atcgcaacaa cggaataaaa
     1201 ttagtttacg aacctgaaca agcgcggctg aaagccaggc aacatgccca tcattttatt
     1261 aacagcattc gcaacaccgg tcgcgaagca gctcatttgg gatatgacca acccctcatc
     1321 atcgcctgct atgataccga actttttggt cactggtggt gggaaggcat tgactggctg
     1381 gaagaggtgg tgcgcggcat tgcccatagc caggaattgg attttatatt gcccgcccaa
     1441 attcactttc ccgacgggga agctcaagtt ttcgaatctt cctggggtac cgggggcaag
     1501 cattatatct ggaaaaacaa agaaacccag tggatgtggg atatcatccg gcaagccagt
     1561 aacgagtttg aagagctgaa taaaagcagt atcgacagtg atgttaaggc tgcttccctg
     1621 gcacaggcgt tacgggaatt gatattgatt gaaagcagcg actggctttt catggtcacc
     1681 aacaacctga cccgcgatta tgctatgaag cgtttttttg aacactatac caaattcctt
     1741 cgtctggtca atgccatacg cgatgaaaac ttcagcgaaa attatgtcga ctggctcatg
     1801 aatgtagaag tggaagatga cttcctctct tttctaaaat aacgcttgtc aggaggtatg
     1861 ttgtgaaagg ttatattgcc ttttgccccc attttcatca accgcatttc caactctata
     1921 aaacccgtga agaagcattt gccaactcgt atcagccttg gctggaactt ttgcaggaag
     1981 ctgccgggct ggataaattc tatatcaacc tgcatttttc cggtcccttt ctttattggt
     2041 tccgccagca aaagcccgcc tatctgcgag attttaaaaa cctcctgaac acacaaaaga
     2101 tcggtctgat cggcggattg gctgacgagc cttttattca gctcagttcc cgcatcgatg
     2161 actgtctgta tcagataaaa aaatatgatg aattatgccg cgaactgttt ggcgttgaag
     2221 cccgcgattg gcagggcatc cacctggtgg aacgtgaatg cggagagtat ttgctgcgtc
     2281 acctcaccca ggcagcatat ttgctgggtg ccccgccgct gtattacctg gacgcggaga
     2341 ccttttatca gccgcacttt tccaggccgg gcggattgta tgattattgc cttaaacact
     2401 ttggtttcaa agatccggta gcgctgacaa ccattgctca tatcccggac gaaatgctgt
     2461 attttggtct gcaagacgag atcggcggtc agatatttca tgcgataccg gtgcataccc
     2521 aattccgtta tcaactgttg aaaagagatg cctttaccgc ggacgatcga gtgcagatca
     2581 aaccttccca ttattacttt tatctcaaag acaccctgga acaggcgcac aagttcgctg
     2641 gtcaatatgg aagagccctc gaccccatcc tggtaatctt cgaggatgcc gagaaaatgg
     2701 gacaatggag taaggacccg cagggcgaca aacagtggtt gatggaattc ttccgtttgg
     2761 tggaagccga tcctgatgta gaatttattg gcctgcaaga ctatatcaac cgggtcggca
     2821 tcctggatac gtatccggta tccagcagcc acagctatcc tgaatgggaa aactggaccg
     2881 ccaaacgagg cattcgcggg gtcacttacg gcgatgaaag attgcggcgt gtcatcagca
     2941 gactgcgcct gtttgaaaaa cagcaggatg cttttgaaca ccatattgtt ggtaatgcgc
     3001 taaaaataac tgcctcggcg gaattaaaag acatgcaaat cagagcgata atgaattctc
     3061 tggagcgcta tgtgtttatt gatcaatatt tggcggaagc ttatcctccg cattaccgtg
     3121 attattatca gatcatcaac cgggtccgga atctggttta ccaagaggat cccaaatggg
     3181 ccagccgcca tccgtcgtat ggttcatccc cattttatga catgcaggga ctggcttatc
     3241 tggaaatcgc ccatcgactc ttgcatcagt tgatgcagga aatgggctgc gaaacaacgg
     3301 aaaataaaag tgccaacctg cttgattggg attttgacgg ccagacggaa gttgttctgg
     3361 ccagtcctgc gcagacgctg gcgattgacc cggtgggcgg ctgcatacaa tatcattttg
     3421 tgctgcgaga tgatctgaca aactttgctg cactggccaa cttgctgcaa caaaactttt
     3481 ccgggctgga tgcttaccat tccgtttacc gctatgccta tccgctgatt atgacggaaa
     3541 ccgacagcag tatgaaggcg gaaatcttcc cggaaggagc ccggcgggaa atctgccgta
     3601 attcgctgcg ctgcagtttg ctaatcgaga acaacggagt ttttacggaa atcggtgact
     3661 ttgatcgtgc cttgtacgaa ttggagggag ttttccgaga ggataattgt tgtcgggcct
     3721 tgctcagttg cacaaaaagc atcaatctca accatcaggc agggattgtc aaactcagca
     3781 aggaatacct gttgcaggac aatggcttgt cgctgaccat caaagctgcg tttgaagaaa
     3841 tctctcccgg caattgtttt ctggcagtac aagtggtcag ctcggcagca ccatccgatg
     3901 aaattgattt tcatccgctg gcctggctgg aactcccggc aggtgagggg aaaccaaatg
     3961 ttgacgtcca gcaggccgtg cagtggaata acggtgaagt ggaatttgtc aacaaaactt
     4021 gttctgctgg cagtttggat caaattgatt acgtctttct aattcatact ggccaagggg
     4081 acctttttgg caaccgtatc tgttatcatt ttaccggcga tgagatcgag aaattggaag
     4141 ttcggccggc tgttgaaaac tattatcagg gtctggtatt tgatcatcaa agcaaactgg
     4201 gctatcaaag ttccggtctg atgctaatac catacattcc agtaaaaaat ggcgtcgcag
     4261 catttaccgt cgatatgcat tggcagcttg atgatgatta cgaacgagtc agcaatcgtc
     4321 agcgccttta tttgatcgat agattttagc agatgatgga aggggcagag aatatgttga
     4381 gaaagttttt tgattattat tttgagcggg ttgaccaaga tgtattgcaa ttataccagc
     4441 aactgatgcc tgattgtaag gacctggcgg aaagctatgc ggccattttt acgcgcaatg
     4501 acgaggaatc gatcaatttt ttacttgatt tctatcgggc tacggctgat ttgcgtcaca
     4561 aaattccgca gcagtcgatc ctattcaata cgcatgacaa tatttgtttt cctttctttg
     4621 ttgagcaggt ctggaatatg ctggatgagc agggccgggc ggctgtcgca gcccgggtgg
     4681 gaaatgatga tatcaatgcc atagtggctg cttttacttc tgacgtagcg ctgcgcaact
     4741 ggttccgcca ggaaattgca gtaaatcttg gacaaaatga tctgttcagt ttaaatgaag
     4801 ttatcgccct gcagaatttt tcttccggcg gggaatcgtc ttttctcaaa tatgtctatc
     4861 cgcatgtgca aaaggtgaac ggacgggtac tggacgcggg atgcggagcc ggttttgcca
     4921 ccatggtgat gagtcagttc gctgatgtgt attccattga tgcctgccag ccacgcctgg
     4981 aaagagcgac ggccttgtcc aacatgatga gaaaaggaga gaaagaattc ttttccggcg
     5041 tctttgctct gattgacgaa gagctgggag atctggcgga gaatgttcat ctgaatgtct
     5101 ctgcagagct gttgaacggc aagccccgcc aggtggagtt cacggttggc agcatcgatg
     5161 cgctgccgta tccggatcag tattttgcca tgatcaactg cctggacgta ctggagcata
     5221 cctatgatcc ggcaaaaata atccagcaat ttgcgcgagt tctacagcct ggcggtcgtc
     5281 tgtttatcac cgtacccacc aaatacgggg aagtggttca gcattactat gaaagactgg
     5341 aaggctccat gtttccagca atgctgcata tgcaccattt tgatccggca accttaaacg
     5401 gaatatttaa acaaaacggt tttaaagaat tccagctgac gccatttgat tatatggatt
     5461 ggccatcttt tgtagaaatt gctgatcaat taccgcaaac taagctggtg gctgatctca
     5521 aatcccagcc agccgaaacc gtagcgctgc agttgtttgc catttacgag aaaatctaat
     5581 tatttctctg gccagggttc ataccatttg taatcaatca tttgcacgcc cgccttactg
     5641 gcggattgtt gattataata attggtggtg ccaaaataca catccaccca ataaggatgg
     5701 gccggccaca gaccgtaata aacgtcgccg gtatcattga ccgtcaattc agtatgggtt
     5761 tgtccttgaa tcgtcactgg ttcctgcaga taaaggatcg ttccaaaagg aataatggga
     5821 ctgcgggctt caccgccgga ctgcaaggga taacgggggt ggacagcacc cgaaccaatg
     5881 aaatacgacc gcccggctga ggaaaccggg gcattagctg cttccgtata ggcagtggtt
     5941 ttttgttgct tgaccgagaa ttcaacatac catttgcccg gcggcttttc gctttgttgt
     6001 cccggtgcag gtttcctgga tggcggcgga gttgactgct tttgtccgct gtaaatgaaa
     6061 tagaatgtta aaaacagaat aacggcaatc aagggaatca tcaggatacg gtaattgcga
     6121 accataatgt cctccgtttt aattttattt tgtgtttttg ctgccgattc tataaaataa
     6181 tgtttcaggt aagtcattgc cgcaaaggaa attgctgtga atgattttca gcaaaggaga
     6241 tgcaatcaat gccgatcgtc aatgcaggca ttagccctca attatttgat tatctggtca
     6301 aatcaatgac tgacagtcct ttttacaaac ttatggagat aaaaattacc cttcttggag
     6361 aaggccaagc cgaattggaa gtccaagctc aagaaaaaca taccaatccg ataggactgg
     6421 tacacggtgg gctgatcatg tcgatggctg atgcggccat gggcaatgcc atacgcagca
     6481 tcggcgtgat aggcgtgaca gtggattgtt ccgtatcgtt ccccggctcg gctcgtctgg
     6541 gagaaaagct gctggctcgg ggaaaagtga tcaaagccgg ccataaaatg atattcgcag
     6601 aagcaaccgt ctgggcaggg gaaagaattc tcggccacag caagtcaacc tttttcaata
     6661 ctggaaagat tgaattaaca taggagagca tgatgagcga ccatctattg aatttgcaag
     6721 aattttattt atttaccttt gtcagcacct cgcatgccct gcaaggcgag cgaatcctca
     6781 aagcccaaca ggcagaattt atcatgattc ccacgctgcg ggagatctca gccagttgtg
     6841 gtttgtctat taaaattcgc ccggagaatt tggcgcaata cagacaaata ttcctggatc
     6901 atcgggtcaa agcggaaaac tattatcatg tccgcaagga tggcaaacaa tacatgatca
     6961 aagcgtttcc ttttaacaca aaagaacaat agcccgacag aaaagatgtc gggctatttt
     7021 acgacaagag gcttgaataa atgaaaaatc cagaccaatc tgcgatggaa aatcaaatcg
     7081 aactgctgtt gggcaacgcg ggtattctgt cccagcactt gccagcgttt cgttttcgtt
     7141 cccagcaaaa gctgctggca gaaaacattg ccgctgcgct ggccgatgac ggctttttgc
     7201 tggccgaagc cggcaccggg atcggcaaga cctttgccta tttaattcca gccattacct
     7261 ggtgtcagca aaccaaaaac aaagtggttg tgtccacgcg caccaaggcc ttgcagcaac
     7321 aaatcaccga ggacgatctg ccggcgctgc tgcgcatcat gaatagccgc atcaaaacag
     7381 ccgaggccaa gggacgggac aattatttgt gctggaacaa atatcagcaa atcctggccg
     7441 gcaaaaaagc gctctctgcc gctgagcagg attttgtcca ggccatcctg gtttgggcgg
     7501 aaaggaccaa aagcggggac cgcaaggagc tggaactgcc cagcgaaatg atgcaatcct
     7561 ggccagtact ggcggctgac cgcaccagtt gtctgcgtga ccaatgccgt tatcaggaca
     7621 agtgttttcg acaaaaaatg atccgcaatg ttcacaaggc ggacctcatt attgtcaatc
     7681 atgccttgct gctctcggat attctgattg aaaaccggga aaaaagcatc ctgcctgatt
     7741 acgaatacct cattatcgac gaagcccata cctttatcaa ggaatccttt gagcaacttt
     7801 cttatcgctt cagcagttat gaaaacaaaa gagtgctggg cgcattgcac gaaaaagccc
     7861 gggggaaagt tcgccgcggc tacttgctgc atctcttgtc cgcccatccc cacttgtctg
     7921 ctctcattac cgaagccacc acctatgctg accagagtca gcgatggatt gatgagtttt
     7981 tcaattcttt gaatgacatc gctgtctaca gtgacaacta tgcttatcac catgtcatta
     8041 aaccggggga actaaatcaa ccggcgctgc agagggctga ccgggccttc cgggaatggc
     8101 gcagggagat ccgcctgctc attgacaagc tcacggaaac cagagatgaa atcgccagcg
     8161 aggatgactt cctggaactg tccggtctca tcaatgcatt gacggaactc ggcattgtcg
     8221 cttccatcat aatggaagat aacctcctcg acccgcaagc tatctgctgg ctggaatatg
     8281 aaaagggtag agtatcctcc atttgttcct ccccaatcta taccggagac agcctggatg
     8341 agaaactata ccgcaaatta aatggactgg tgatgttgtc tgctaccatg acggttgaca
     8401 acaacttcga caattacatc aatcgcagcg gtttgcgttg gtatgaacag gacggacgac
     8461 tcaataccat gatcgaaacc tctccttttg attatgaaaa caatgctgcc ctttatattg
     8521 ttgaagatat gcccgatccc ggcaacactc gttttttcac tgaactgcag cgggtgctga
     8581 ccgagattgt cctgctcagc cggggtcgca ccatggtgct ttttactgcc cgcaagcaga
     8641 tggaggaaac ggctgcctgc ctgcgtcctc tcttggaggc caatgacata aaactgcttg
     8701 tacagtacca agatggagag tttgccagtc tgatggatgg ttttaaaagc catgcaaatg
     8761 ctgtcttaat gggcttggaa accttctggg aaggcatcga tttaaaaggc gaagtactga
     8821 aatgtctggt catcgtcaag cttcctttcc gttcaccctc cgatccttat tgtactgctt
     8881 gggagaaaca ctacctgcag cagcgtatga acagctttga acatttcatg ctgcctgatg
     8941 ccaccattcg tctcaaacag ggagtcggca ggctgattcg cagcgagacc gaccggggac
     9001 tcgtgctggt cctggacacc cgtctgattt acaaaaaata tggacaattg atgcaaaaca
     9061 gcctgccgtt gaccaatata aatgccgtct cttccgcaaa actggccgaa atcatcaaac
     9121 catggtttta atcttcctcc aaatgtgaac attttttaac gccgcttcat agtataaggc
     9181 ataaataacc tggctatgaa ggggtgattg acgtgcgggt tttttatttt cggatcccga
     9241 tttttgtaag agatatttta ttgaaaatat gggggtaaaa caggatatct gtttacgtcg
     9301 tcaggctgaa cctggcagat gatgagcagc gctgcaagcc aaggagggta aatcatgtgt
     9361 aatccaggtc tgattttggg attattattc ctattggccg gattcatcct ctggctttgc
     9421 agttgttgag ctacgttgat aaataccatc atgagcaaca caaaaagagc ccccggtata
     9481 gaccggtggg ccttttttta ccgtgttgtt attcagcatt taacccgatg cccagtatcc
     9541 cgccaaatcc ccctgccgcc aggcaaatga gcaggttgat cagataattc tggatatcga
     9601 tcccgcctgt ctgaaaaaca agagaggcga tcagtaaaat gatgtaataa atgatgccca
     9661 tggtaaggcc gcgaatcaag cctttattgc catatgattt gcttacatga caaccagcat
     9721 agaatacgga accaattaca atcagctggg cgatccatcc agctgaagtt tcctccagtg
     9781 cggaaaagta caagaccagt gccgccgcca aacaagccac gattcccaac agtaatgatc
     9841 gcagcaaacc ctgcaactct gccgactttc caatattcat tcaaaccacc ccctcatttt
     9901 gtaaaatatg ccaccccctc cataaatatg tctgaacata aaaaaaccta taataaccag
     9961 tcccaacata aatatttaag caggggagtg gttaatatga ataatgcttt atggtggagc
    10021 tttttggccg gattcagtac gagcatcggt gccgctttac tcttccttaa taaaaaatgg
    10081 cgcccccgtc atttagctta ttttctgggt ttggctgcgg gtgtcatgac tgccgtcgtg
    10141 ttttttgata tgctgccttc agccttggtc agcggcagca gcaaagcttt gtcaggcgtt
    10201 gtaattggtt tgttgttgat gtttctgact gccgggatac agcatcaggg aatacattca
    10261 acccaaacgc tggttggctt gggctatatg attatgctgg gcattgcctt gcatgatttg
    10321 ccggagggga tggcggttgc tttgggagaa cacatgaaaa accgtaccgg gatggtgatt
    10381 gccctgggga ttgggattca taatatgccg gaaggcatgg ccattgccgc cccgctttta
    10441 atgagcggaa tgaaaaaaag tatggttttg ttgcaaacct tgctgatctc cttgattaca
    10501 ccgctgggta ccctgctggg cagtttcctg ctccatctcg ccccgggatt gatgcccctg
    10561 atgctgggaa ttgcctccgg agtcatgttg tacctggtca tttttcaatt gtggccccaa
    10621 gccggttata aaaataaatc cagtcgatgg cccggattct gtctgggaat gctggtaatt
    10681 gggttggcga cctacctgta aacctgagga ttgaaaattt atttaccaat taagactttc
    10741 caacgataaa acatatttac tataggtata tttattaata tacttgatat aaataagctg
    10801 tggttcaagg ctaaattgtg ctatagtcaa tattaagaaa aatgatagcg cagattcatt
    10861 gcaaaaagaa aggaaggaaa acaatatggt attattcaat ccccacaact acaataaaga
    10921 gcatcttgat ccccgcgccc gggaaatcat gctcaagacc atcgactttt ttgaagacaa
    10981 aggtctggcc agcataaagg aagatgacaa gaactttacc tggtatcagg actatgttga
    11041 ttttatcggc aaagaagaaa tttttgctga tttacttacc ccggccggct acggcaagga
    11101 agactcccgc tgggaccttt ggcgggtagc agagtttaat gaagtttccg gcttctatgc
    11161 cctccatttt caatatctct atcaggtaac catccttggc ctgggtccga ttttcctcgg
    11221 caacaatgaa gcggtcaaac acaaagcggc ccaattgcta agagaaggcg gcatcttcgg
    11281 atttggtctt tcggaacggg atcacggcaa tgacatttac tccgatgaaa tgacacttta
    11341 tccgaatggc gatggcacct atcgggccaa aggcaacaaa tattacatcg gcaacggcaa
    11401 caaagcagct ttgttgtcgg tattcggaaa atttgccgat accggtgaat atgttttctt
    11461 tgcggtagac accaaacatc ctaactatga actggttaaa aaaatcaata cgaatggcgc
    11521 acattgcgct tatgtggcgg aattcaatct caatgattat ccggttaccg atgcggacat
    11581 tctttcccgc ggaccgcatg cctgggattc tgccctgaat acggtcaaca tcggcaaatg
    11641 tatggtcggc tgggcttcca tcggcgaagc cacccatgcg ctctacgaat ctctgcacca
    11701 cacctgcaac cgccagcttt atggcaaacc ggtttatgct ttcccgcatg tcagaaggtt
    11761 gtttaccgag tcatacctgc gtctggtgac gatgaagctg tatgcgctgc gctgcatgga
    11821 ttatttccgt tctgcttccg atcaggatcg ccgctatttg atgtacaatg ccatcctgaa
    11881 aatgaaggtc tgcagccagg gggataaaat ttcccaaatg ctgcttgata ttgtaggcgc
    11941 caaaggcatg gaacaggata cctttatgga attattcatc cgcgacgccg gcatgatgcc
    12001 gcgtctggaa ggcaccaccc acgttaacat ggccttggtc aataaattct ttaaacccta
    12061 tttctttggt ccggtggact tcccgcatat ccccaaacgc aatgacattg ccaacgacaa
    12121 ttatgtttac aaacagaaag ccggcggctt aaaggaagtt cgcttcggcg attacagcct
    12181 ttgttatgaa gggttcaatc aggctaatgt cttaaaattc cgggagcaag ttgaattgct
    12241 caaacatttc ctggccaaca atacaccttc tcccgaacag gccgccaatg ttgattatat
    12301 gatcgcgctg ggcgaactgc tggctgccac cgcttatgcc caattgacat tggaaaatgc
    12361 caagatttac ggtatcgaag atgatgtgct ggaagaaatc tttgccttta tgattcgtga
    12421 tttcgcggct tatgccttga tgttcagatc aaacttcgta caagggccgg atcagctcga
    12481 gaccatcaac aaaatgatca tcgatcctgt gcaggacccg gcccgctttg ataaaatttg
    12541 ggaagagaga gtcttgagcc taaaagacgc ctatgtaatg aacgattaaa agctatttta
    12601 taaccaacag cccgatgggt aacaatgttg ccgatcgggc tttttttggt gcgcccggca
    12661 cgggcgcagt ctaatgggtg aaagtcccta gtgc
//
LOCUS       DAOTHZ010000061        12638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5942_length_12638_cov_8.355003, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000061 DAOTHZ010000000
VERSION     DAOTHZ010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_12638_cov_8.355003"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1834
                     /locus_tag="P5549_07720"
     CDS             2..1834
                     /locus_tag="P5549_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW12998.1"
                     /translation="MSYKYSYNTRDIKFILKEWLPFDEVLTWDRYKDYYSMDDVDVIV
                     DQVRKVAEDIIAPTADDADAIGAQWKDGQVYLPPSFAPAIRFMHEQGWGASNFDWDTE
                     GGIPLTMYFLLWEMCSAANPALMSYISNGGAVSNVIYHYAGDELKKKFLPKLYDGTWG
                     GTMCLTEPSVGSDVGDMSSKAFPTDDPRIFKCKGQKIFITVGDRDDVDNIIHLYLARI
                     EGAAKGTKGLSLFIVPKFWINEDGSVEDNDVQTAGIEHKMGLKGSATCQLVFGEENNC
                     RGWLIGNPPDESGIGQGIVQMFNMMNEARLDTGRLATCVAANAYWAAKDYGKDRVQGA
                     LISNPKAGRVTINQHEDVKRMYLLNKATTEACRALLAKCYYYEDGREFAPTPEMRKFC
                     QTKLDMLTPLCKAYPSDEAWGLIAESLQSYGGYGFCEDYPAANSARDCKIWSIWEGTN
                     YIQSLDLIGRKWMQGKGAHFAGILKEVEDFIAENKGNFGALEKELAHLEKALTAYRQI
                     QFAIGGYVQGGKIGLMAIYARRILTATAQLYCGYLLLEQAKIAVKRMEELGADHYDYN
                     FYLGKVFSARYYCNNAVPNVWHILELIQIGDTSAMEAPVEIFEY"
     gene            2149..3498
                     /locus_tag="P5549_07725"
     CDS             2149..3498
                     /locus_tag="P5549_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW12999.1"
                     /translation="MINSFRRQYEEKLISAEKAAEMVKSGDKIFYSEFAMFPKLLDEA
                     LAERISELHDLELYSVFYTQVPKVIEADPKHEHIVTQDWHFGPVSRRLADEDKCFYVP
                     ISYHQGPRIINKYIENDVAMVAVAPMDAKGFFNLGPTNSVTPAYLNKSKLRILEVNSS
                     VPHCLGGNSENIHISDVDYVVEGENYSLIQLPTAKPKEFDHQIADYIMNEIEDGACLQ
                     MGIGSMPNLVGAKIAQSDLKDLGIHTEMLVDSCIDLYESGRVTGNRKSIDRGKMVYSF
                     ALGTDKLYKFMDNNPICASYPVSYTNDPRMIALNDKVVAINNALEVDLLSQVSSESSG
                     YRHISGTGGQLDFIMGAFSSRGGKGFICLSSTHSDKNGNLHSRIVPSMKVGSVVTVPR
                     SMVQYVVTEYGIVQLKGKSTWKRAEALISIAHPQFQDELVKAAQEMKIWVRSNQIEA"
     gene            3891..6302
                     /locus_tag="P5549_07730"
     CDS             3891..6302
                     /locus_tag="P5549_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HRW13000.1"
                     /translation="MKKRIRKVAILGSGTMGGGIAALLAAAGIRSYLLDIVPRELTDK
                     DKAAGLTVDSPAFRNKIAEGNKALLLKSKPAQFQTKADADLVTVGNTEDHIGLLAECD
                     WVVEVVPEKMEIKKAVLKNIAANVKPGTFVTSNTSSISINKIVEDMPLEFRQYWMGTH
                     FFNPVRYMKLLELIPGKDTLPEVVDFFAEFGERVLGKGIVYAKDTPAFVANRLGNWAG
                     PSCTRLMAELELTVPEVDALTGSAIGRPATGTFGLFDMVGVDIAVLSCLEVKHNVSDP
                     AEQALYTPADFLQQMLEKGILGNKTKGGFYKRVVVDGKKVKQVLVDLEKFEYGPVVKP
                     DLASLAAAKKAPTLGAKLEAFFESDDKGGQFVWKHVSGLFLYAASKIPEVSDDILNMD
                     RALKWGYNHSKGPFELFSEVDLPKYIARMKAEGMAVPAWIDEMLAAGITSFYKTEAGV
                     EYYYDIPGKKYVPVAVSPSAIILPKLKAENKVVSETPSGTIYDMGDGVLCLDTHSMAN
                     CVTADLTATIIAAHDILAKPEWDGMVISGAGKDFMGNVTDYFTMMNLANEKKFDAIEE
                     IIRQGQKAFDLNKYSLKPVVVAPKGKALGGGCELIYMSSAVQALGETYAGHVELGFGL
                     MPGFGGMKEAVLRVVNKIAGSKAHPADFLQGEFETLAMAKVGTSAKEVQKLGFIKATD
                     GITLNEDFLLGDAKQKVLDMLDNGWSAPVKRPFGAFGLTQVALLKVGTFQMKQAGVLS
                     EYDWHIACKMTDVFAGGSMQAGTPITEDYINDLTVEAILSLIGEQKTLDRIMSMVTKG
                     KPLRN"
     gene            6350..7531
                     /locus_tag="P5549_07735"
     CDS             6350..7531
                     /locus_tag="P5549_07735"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRW13001.1"
                     /translation="MREAVIVSGVRTAGGKAPRGSLKDTRPELMSKLVMQEAMKRAGG
                     KFEAGMIDDIIWGCSTPEQACGMNHARNSAIYADIPYQVPAVTVNRFCSSGVQAISMA
                     SEAIMTNRTDIMMAGGTEHMSMIPMGGIVRPNNEMALHPFWATVYTSMGQTAEHVASR
                     YNISREDQDVFAAASHAKALAAREKFKEEIVPVPISTTTLGADGKPVTKVVMYDYDEG
                     AREGVTAESLGKLKPAFTPTGVVTAGNSSQTTDGSACVILMTPEKAKELGCKPRLKYV
                     GYAVAGCKPDEMGVGPYYAIPKALKRTGLELKDIGLIELNEAFASQALYCIRELGINQ
                     EICNVNGGAIAIGHPLGCTGAKLTVTLMHEMERRNVKYGIVSMCIGGGMGACGIFELC
                     K"
     gene            7865..8641
                     /locus_tag="P5549_07740"
     CDS             7865..8641
                     /locus_tag="P5549_07740"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRW13002.1"
                     /translation="MGIKDSFDLTGKTAVVTGGSIGLGAQMAAGLAEAGANLVIAARK
                     VERCVELCAKLEENGIRAIAVACDVSDAASCQNLVDITVKEFGTLDILVNNAGYTWGA
                     DAMNYPMDKWQQILDLNLTGLFQLSAMAAKVMKEQGRGKIINVTSVAGTGGCAPEAQN
                     TVAYNASKAAVDTLTKDLACKWARYGIYVNAIGPGFFPTHMSGGLLEKNQHLILPRVP
                     LNRLGGEEDLKGTIVFLSSAASDYITGQILYVDGGQTAMI"
     gene            8983..10416
                     /locus_tag="P5549_07745"
     CDS             8983..10416
                     /locus_tag="P5549_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRW13003.1"
                     /translation="MRTSKEYLEKLAKMRPNIYLDGECIGRDHPKVVAASKSIQKTFD
                     LANDDHYGKWLTTTSHISGKPINRFCHIHQSVDDLLMKQEMTRVLCNLVGGCIQRCMG
                     TDSMNGLSVATKNADLKYGTNYHERWLKYLEYFQENDLVGAASQTDAKGDRSKRPAEQ
                     DDPDMYLRVVERREDGVVVRGAKLHNTMAPYCDELLVFPTRMLGKNEKDYAIAFAVPA
                     DTEGVYLIARQAFSPTREPGMEAPYTELADIESMTVFDNVFVPNERIFLNGETDIGGQ
                     LALLFALFHRHSYCGCKPGIGDVMLGTTALLADYNGVEKTGHTQEKLADVISVVELVY
                     AAGIAASVKSTKAPSGTQVPNVIYCNVGRRHAGHNVYHEYDILCDIAGGLPGTLPMTK
                     EYNNPVIGDFVRKYTKRRTGVSEEDHYRAYHLASDILTGEVGAVLLVAGVHGGGSPIM
                     EDIAIMSTYDIKAKKNLAKFLAGIKQD"
     gene            10561..11625
                     /locus_tag="P5549_07750"
     CDS             10561..11625
                     /locus_tag="P5549_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRW13004.1"
                     /translation="MEEKTMKALVYHAPHQFGLEDVPVPQIMAPDDAIIRVTLATPCT
                     SDIHILEGHMPFVKGPITLGHEFCGEIVELGAAIQGYEVGQKVHCLPGAFCNQCDMCK
                     SGYSSFCETGGCYGTVKGLNGALAEYIRIPHADQSMIIIPDGLLEEDVLLLGDMLGTG
                     YFGVTNGEVKKGDAVAVFGVGPVGMCACILAKKVFEADPVIAVDLAQYRLDAVLKAGV
                     ADYVINPNEEDAVKKIREITGRRGVNAAIETAGVQSTMTAAAKSLAIGGILSTIAIFA
                     KPVELPLHIMPNRNNQLRTGIQQCAGMDVMLEEIKAGRINTRFMQTHRSPLNDIIHAL
                     DIFGGNKDGCIKYLITPFER"
     gene            11813..12586
                     /locus_tag="P5549_07755"
     CDS             11813..12586
                     /locus_tag="P5549_07755"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRW13005.1"
                     /translation="MKEINSQAQPFYFTGNSQQALLFLHGFTASPSEVRVVAEKIHQS
                     IGCSVSGILLPGHGTTPEQLDAVRWPEWYQAVETEIKSLQASFQQVFVGGLSMGGLLA
                     LHAGVNLSGLAGVVTINAPIYYHYAVIPLVSDLTGLITPFYHKKGLAEIKKLENQGRF
                     AYRVMPLKAFHSLNHLRKIVMQEVKELKIPALIVQSMLDESVHSRSAEYLYEKTRPQG
                     ARTLLLSQSRHIATMGPQQEIIVQEIVSFMEDQRCFLGK"
BASE COUNT         3504 a         2918 c         3230 g         2986 t
ORIGIN      
        1 agtgtcatac aaatattcat ataacaccag agatatcaaa tttattttaa aagaatggct
       61 tccctttgat gaagtactga cctgggacag atacaaagat tactacagca tggatgatgt
      121 cgatgtgatc gtcgatcagg tccgcaaagt tgcggaagat attattgccc ccactgccga
      181 tgatgcagat gccatcggtg cccaatggaa agacggacag gtttatctgc cgccttcctt
      241 tgcgccggcg attcgtttta tgcatgaaca gggatggggt gccagcaatt tcgactggga
      301 taccgaaggt ggcattccgc tgaccatgta cttcctcctg tgggaaatgt gctccgctgc
      361 caacccagcc ttaatgtcct acatttccaa tggcggtgcc gtatccaacg taatttatca
      421 ctatgccggc gatgaactga aaaagaaatt cctgcccaag ctgtatgacg gaacctgggg
      481 cggaaccatg tgcctgacgg aacccagcgt aggctcggat gttggcgata tgagttccaa
      541 ggcattcccc accgatgatc ccagaatctt taaatgcaaa ggacagaaaa tttttatcac
      601 cgttggtgac cgtgatgacg tggataatat catccatctc tatctggccc ggatcgaggg
      661 agctgccaaa ggaaccaaag gactgtccct ctttatcgtc ccgaaattct ggattaacga
      721 agacggcagt gtagaggaca atgacgtaca gacggccggc attgaacata aaatgggact
      781 gaaaggatcg gcgacctgtc agctcgtatt tggggaagaa aacaactgcc gcggctggct
      841 gatcggcaat ccgcctgacg aaagcggcat cggccaggga atcgtgcaga tgtttaacat
      901 gatgaatgaa gcccgtctgg ataccggtcg tctggctacc tgcgttgctg ccaatgccta
      961 ctgggcagcc aaggactacg gcaaagacag agttcaggga gcccttattt ccaatcccaa
     1021 ggccggacgc gtaaccatca accagcatga agacgtgaaa cggatgtatc tgttaaataa
     1081 agccaccacc gaagcctgcc gggccctgct ggccaaatgc tactattatg aagacggcag
     1141 agaattcgct ccgacaccgg aaatgagaaa attctgtcag accaagctgg acatgctgac
     1201 accgctctgc aaagcctatc cttcggacga agcctggggt ttgattgctg aatcgctgca
     1261 gtcctatggc ggatatggct tctgtgaaga ctatcctgct gccaattcag cccgtgactg
     1321 caaaatctgg tcgatttggg aaggcaccaa ctacattcag tctttggatc tgatcggccg
     1381 caaatggatg cagggcaaag gcgctcattt tgccggcatt ttaaaagaag tggaagactt
     1441 cattgcggaa aacaaaggga actttggcgc attggaaaaa gagctggctc atttggaaaa
     1501 agccctgact gcctatcgcc agattcagtt tgccatcggc ggttatgtac aaggcggaaa
     1561 gattggtttg atggccattt atgcccgtcg cattctgacg gctacagcgc agctgtattg
     1621 tggttatctg ctgctggaac aggccaaaat agctgtcaaa cgcatggaag aactgggcgc
     1681 agatcattac gactacaatt tctatctggg caaagtcttc tccgctcgtt attattgcaa
     1741 caacgctgtt cccaatgtat ggcacatcct ggaactgatc caaatcgggg atacttcagc
     1801 catggaagct cccgtggaga tctttgaata ttaaaaacta gacctgtccg acatatgacc
     1861 cgccggttct ttttacaagt atcggcgggt cccccctttt tatcccatat atgtgctccg
     1921 gcaagcctgg ggcaggattt caattaagaa attataaaaa tcaccatata aatagaggga
     1981 atttgtattt tatagcgaag tattcaatat agtaaaaaaa aagtttgtgg aaagcgaaaa
     2041 aaaataataa taaatcaata ttaatagaaa gctataaaag atataatcat attaatccaa
     2101 gcattcatta atgcaataaa tctattttag caggagaagt gatttagtat gattaactct
     2161 tttagaaggc agtacgaaga aaaacttatc agtgcagaaa aagcggcgga aatggtcaaa
     2221 tcaggagaca aaatattcta cagtgaattt gccatgttcc ccaagctctt ggacgaagca
     2281 ctggcggaac gcatctctga acttcatgat ttggaacttt acagtgtttt ctatacacag
     2341 gttcccaaag ttattgaagc cgacccgaaa catgagcata ttgtaacgca ggattggcat
     2401 tttgggccag tttcgaggcg acttgccgat gaggacaaat gtttttatgt tcccatttct
     2461 tatcatcagg gccctagaat catcaacaaa tacattgaaa atgacgtagc catggtggca
     2521 gttgccccaa tggatgcgaa aggattcttt aacctgggac cgaccaattc cgtaacaccg
     2581 gcttatttaa acaaatcaaa attaagaatt ctggaagtaa acagttctgt gccgcattgt
     2641 ttgggtggca acagtgaaaa tattcatatc agcgatgttg actacgtcgt cgaaggggag
     2701 aactattccc tgattcaatt gccgacggcg aaaccaaaag aatttgatca tcagatcgct
     2761 gattatatta tgaatgagat cgaagacggt gcctgtttgc aaatggggat cggcagtatg
     2821 cccaacctgg tgggcgccaa aatcgcccag agcgatctca aggatcttgg tatccatacg
     2881 gaaatgctgg tggactcctg catcgattta tatgaatccg ggcgggtgac cggcaatcgc
     2941 aaaagcatag accggggaaa gatggtctac agtttcgcct tgggtactga caaactctat
     3001 aaatttatgg acaacaatcc aatttgcgct tcctatccgg tcagttacac caacgacccg
     3061 aggatgatcg ccttgaacga taaagtggtt gccatcaaca acgccttgga ggtggatttg
     3121 ctttcacaag tcagctcgga atcgtccggt tacaggcata tctccgggac aggcgggcag
     3181 cttgatttca ttatgggagc attttcttcc cggggcggca aaggttttat ttgtttgagt
     3241 tcgacccaca gtgacaaaaa cggcaacctt cattcacgca ttgtgccgag catgaaggtc
     3301 ggctcagtgg taaccgtgcc gcgctccatg gtgcagtatg tggttacgga atacggcatc
     3361 gtacagctaa aaggaaaatc aacctggaaa cgggccgagg cgctgatcag cattgctcat
     3421 ccgcaattcc aggacgaact ggtaaaggca gcccaggaaa tgaaaatctg ggtgagaagc
     3481 aatcaaatcg aggcataaat cgaaagccaa tggattcggg taaacgtata aacaatcgtt
     3541 gctgttgctt ccagcattgt gacgttatgg cacattttgc tgcacaagtg atggcacatt
     3601 gtttccctgg atatatcgtt acaaaattca caaactaata ttgtaatcca gccggaaagc
     3661 aactgaggag cacgggaaca aagtaaaatc gcagcagcaa acacacagtc gactgcatgt
     3721 agatttcact ggcacaggaa ttgcaatatc agtctataga ggtcccgaac gaattcgaga
     3781 ggggggatgg gtcagaggct agaaaggttt cactgacgga aaacgtcaaa tctgatctca
     3841 tttagaacag gctaaccttt gtggattagt aagattggag gtaaaagaat ttgaaaaaga
     3901 gaattcggaa agtcgcaata cttggttctg gaaccatggg tggaggaata gctgcattat
     3961 tagctgctgc cggaattcgc agctatctgc ttgatatcgt tcccagagag cttactgata
     4021 aagataaagc ggcaggcttg actgttgaca gccctgcatt cagaaacaaa atcgccgaag
     4081 gcaacaaagc ccttctgctc aaatccaaac cggcacagtt ccagaccaaa gcggatgctg
     4141 acctggtaac ggtgggtaac accgaagatc atattggcct tctggcagaa tgtgactggg
     4201 ttgttgaagt tgttccggaa aaaatggaaa tcaaaaaggc agttctgaag aacattgctg
     4261 ccaacgtcaa acccggtact tttgttacca gtaacacttc cagtatttcc atcaacaaaa
     4321 tcgttgagga tatgccgctg gaattcagac agtactggat gggaacacac ttcttcaatc
     4381 cggtaagata tatgaaatta ttggaactca tccccggcaa agacaccctg cccgaagttg
     4441 ttgatttctt tgctgaattt ggcgaaagag tgctcggtaa aggcatcgtt tatgccaagg
     4501 acactccggc attcgtcgcc aaccgtctgg gcaactgggc tggaccttcc tgcaccagat
     4561 taatggccga actcgaactg accgttccgg aagttgatgc gctgactggc tccgccattg
     4621 gtcgtcctgc aaccggtact ttcggtctgt ttgacatggt tggtgtagac attgccgtac
     4681 tgtcctgcct ggaagtaaaa cacaatgtat ctgatccggc tgaacaggct ctctatacgc
     4741 cggctgattt cctgcagcag atgctggaaa aaggcatcct gggcaacaaa accaagggcg
     4801 gtttctacaa gagagtcgtc gttgacggca agaaagtaaa acaggttctg gttgacctgg
     4861 aaaaattcga atatggcccg gttgtaaaac ccgatctggc cagtttggca gctgccaaga
     4921 aagccccgac cctgggtgcc aaattggaag ctttctttga atccgatgac aagggcggcc
     4981 aatttgtatg gaaacatgtc agcggcctgt tcctgtatgc agcttccaag attcctgaag
     5041 tatctgatga catcctcaac atggaccgtg ctcttaaatg gggttacaac cactccaagg
     5101 gcccgttcga actgttctcc gaagtggatc tgccaaaata cattgctcgc atgaaagccg
     5161 aaggcatggc agtacctgcc tggatcgacg aaatgctggc cgctggcatc acttccttct
     5221 acaagaccga agccggtgtt gaatactatt atgatatccc cggcaagaaa tatgttccgg
     5281 ttgctgtaag cccgtctgcc atcattctgc cgaagctgaa agccgagaac aaagtagtta
     5341 gcgaaacccc cagcggcacc atctatgaca tgggcgacgg cgttctctgc ctggataccc
     5401 acagcatggc caactgtgta accgctgatt tgactgcgac cattattgcc gcgcatgata
     5461 tactggccaa acccgagtgg gatggcatgg ttatctcagg tgcaggcaaa gacttcatgg
     5521 gcaatgtcac cgactacttc accatgatga acctggccaa tgaaaagaaa ttcgatgcca
     5581 tcgaagaaat tattcgccaa ggccagaaag ccttcgatct caacaaatac agcctcaaac
     5641 cggttgtggt agctcccaaa ggcaaagcct tgggcggcgg ctgcgaactg atatacatgt
     5701 cttctgcagt acaggcttta ggtgaaacct atgccggtca tgtggaactg ggcttcggct
     5761 tgatgcctgg cttcggcggc atgaaagaag ctgtcttgag agttgtgaac aagattgccg
     5821 gcagcaaagc acatccggct gacttcttac agggcgaatt tgaaaccctg gccatggcca
     5881 aagttggcac cagcgccaaa gaagttcaga agctcggctt cataaaagcg acggatggga
     5941 ttaccttgaa tgaggacttc ctgctgggcg acgccaaaca gaaagtgctt gacatgcttg
     6001 acaacggctg gtccgcaccg gtgaagagac ccttcggagc ctttggtctg acgcaggtag
     6061 cgctgctgaa agttggtacc ttccagatga aacaagccgg cgttctgagt gaatatgact
     6121 ggcacattgc ctgcaagatg acggatgtat ttgccggcgg cagcatgcag gctggtaccc
     6181 cgatcacgga agattacatc aatgatctga ctgtggaagc catcctgagc ctgattggcg
     6241 aacagaagac gctggacaga atcatgagca tggtaaccaa gggcaaaccg ctccgcaact
     6301 aagctgattt gagaaccagt caacatattt tttcgaggag gtaataagat tgagagaagc
     6361 tgttatagta tcaggtgtcc gcacagccgg aggcaaagct ccgagaggat ccttaaaaga
     6421 taccagaccg gaattaatga gtaaactcgt tatgcaggaa gccatgaaaa gagccggcgg
     6481 caaatttgaa gctggcatga tcgatgacat catctggggt tgctccactc ctgagcaggc
     6541 ttgcggtatg aaccatgcca gaaattcggc catctatgcc gacattcctt accaggttcc
     6601 tgcagttacc gtaaaccgct tctgctcctc cggcgtacag gccatttcca tggcgtctga
     6661 agcaatcatg accaatcgta ccgacatcat gatggccggc ggaactgagc acatgagcat
     6721 gattcccatg ggcggaatcg tcagacccaa caacgaaatg gcactgcatc ccttctgggc
     6781 caccgtctac accagcatgg gccagaccgc tgagcatgtt gcatccagat ataatatttc
     6841 cagagaagac caggatgttt tcgctgctgc cagccatgcc aaagcacttg ctgccagaga
     6901 aaaattcaag gaagaaattg tacctgtacc gatcagcacc acgacattgg gcgcagatgg
     6961 aaaaccggtt accaaagtcg ttatgtatga ctatgacgag ggtgccagag aaggcgtaac
     7021 tgctgaatcc ttgggcaaac tgaaaccggc ctttacaccc acaggtgtgg taaccgccgg
     7081 aaacagctcc caaaccacgg atggttcagc ctgcgtgatt ctgatgactc cggaaaaagc
     7141 caaggaactc ggatgtaaac cacgtctgaa atatgtcggt tatgctgtag ctggctgtaa
     7201 accggatgaa atgggtgttg gaccgtatta tgccattccc aaggccctga aacggaccgg
     7261 tcttgagctg aaggatatcg gcctgatcga gttgaacgaa gcgtttgctt cccaggcctt
     7321 gtattgcatc agagaactgg gtatcaacca ggaaatctgt aatgtcaacg gcggcgccat
     7381 cgccatcggc catccgctgg gctgcaccgg tgccaagctg accgttaccc tgatgcatga
     7441 gatggaaaga cgcaacgtga aatacggtat cgtttccatg tgtatcggtg gcggtatggg
     7501 cgcttgcgga atctttgaac tctgcaaata agcctgtagg cgacaatttg atttagttat
     7561 cccccgacga gcggtatggt tcacaccata ccgctcgttt tttgtgcaaa tgttacactg
     7621 tgtaacgaaa acgattggtg atgacggaca ggttttcggc gaatacgctg tgaaaaccgg
     7681 tcaaaacgga gtttttacga agccagtggt ctatgctgca tttggcataa atattgcaaa
     7741 caataataag cagagaaatg acgagtacgt aaaggtgttg aacgaatagc gggggtgagc
     7801 gacgagacaa aaacaacatg ggcatatgat tgtttttata tagaaaaaga atggagggca
     7861 acacatggga ataaaagata gttttgattt aacaggtaaa acggcagtcg taaccggcgg
     7921 ctcaatcggt ctgggtgcgc agatggctgc cggactggca gaagctggcg ccaatctggt
     7981 catcgctgcc cgcaaagtag aacgctgcgt ggaactctgc gccaaactcg aagaaaacgg
     8041 catacgggcg attgcggtcg cttgtgatgt cagtgatgcg gccagctgtc agaacctggt
     8101 tgacattacc gtcaaggaat tcggtacact tgacattctg gtcaacaatg ccggttatac
     8161 ctggggagca gatgccatga actatcccat ggataaatgg cagcagatcc tggatctgaa
     8221 tctcaccggc cttttccagc tctctgccat ggctgccaaa gtcatgaaag aacagggccg
     8281 aggcaaaatc atcaacgtta cctctgtagc cggcacaggc ggatgtgccc ctgaagcaca
     8341 aaacacagtg gcctacaatg ccagcaaggc ggcggtggat accctgacca aagacctggc
     8401 ctgcaaatgg gcacgttacg gcatctatgt caatgccatc ggtccaggct tcttccccac
     8461 ccatatgagc ggcgggctgc tggagaaaaa ccagcatttg atcctgcccc gggtaccctt
     8521 aaaccgcctt ggcggagaag aagatctcaa aggcaccatc gtgttcttga gctcagctgc
     8581 ttccgactat atcaccggac agattctcta tgtggatggc ggacagacag ccatgattta
     8641 atgtaaatca cataatgcca taatgacaca aattgtgtct ggtagattgg agaatttctt
     8701 caaacccttg caaaggattt cgccaaccta tccaatcaaa caatttatga gcattgaaca
     8761 ggatttgtct ctgccgaatg tgtttaaatc gactaaattt aaattattct gtcaatatat
     8821 ttattccaat cgattggttc gcagatattt cacaatataa ttaaatattc caaaaacatt
     8881 cgcgtagaga gatttctctt ctctttggca caaaatgtgt tatcatgaac ctagacacaa
     8941 ttatcgttat caaaatttat atttatatag gagggcgaat gaatgagaac atctaaagag
     9001 tatcttgaaa agctggccaa aatgagacct aatatttatc tggacgggga atgcatcgga
     9061 cgtgaccatc ccaaggttgt agcagccagc aaatccattc agaagacttt tgatttggcc
     9121 aacgacgatc attatggaaa atggctgacc actacctcgc atatttccgg aaaacccatt
     9181 aaccgcttct gccatattca tcagtcagtt gacgatctgt tgatgaagca ggagatgacc
     9241 cgtgtattgt gcaacctcgt gggcggatgt atccagagat gcatgggaac cgactccatg
     9301 aatggattgt ccgtagcgac aaagaatgcc gacctgaaat atggaaccaa ctatcacgaa
     9361 agatggctga aatatctgga atacttccag gaaaatgatc tcgtcggtgc tgcttcccag
     9421 accgacgcca aaggcgaccg cagcaagaga ccggccgagc aggatgatcc agatatgtac
     9481 ctgcgggtag tggaaagaag agaagacggt gtagttgttc gcggcgccaa gttgcacaac
     9541 actatggcac cttattgtga tgagctgttg gttttcccga cccgtatgct gggcaagaat
     9601 gaaaaagatt acgccattgc atttgctgtt ccggcggata ccgaaggcgt ttacctgatt
     9661 gcccgccagg cattttctcc gacgcgtgaa cctggcatgg aagctcccta taccgaactg
     9721 gctgatattg aaagtatgac tgtatttgac aacgtatttg tgccgaacga gagaattttc
     9781 ctcaatggtg aaaccgatat cggcggacag ctggcattat tatttgctct tttccataga
     9841 cacagctatt gcggctgcaa accaggcatt ggcgatgtca tgctgggtac gactgcactg
     9901 ctggctgact acaatggagt tgaaaaaaca ggtcacaccc aggaaaaact ggcagatgtg
     9961 atcagtgtgg ttgaactggt gtatgctgcg ggtattgccg ccagcgtgaa gtccacgaag
    10021 gctccttccg gaacgcaggt tcccaacgta atttattgta atgtgggcag acgtcatgcc
    10081 gggcataacg tgtaccacga atatgatatt ctctgcgaca ttgccggcgg acttccgggt
    10141 acgctgccta tgaccaagga atacaacaac ccggtgatcg gagatttcgt acgcaagtac
    10201 accaaacgtc gtactggcgt ttccgaggaa gatcattaca gagcttacca tttggccagt
    10261 gatatcttaa cgggtgaagt gggagcagta ttactggtcg cgggcgttca tggcggcgga
    10321 tcaccgatta tggaagacat tgccatcatg agcacgtatg atatcaaagc caaaaagaat
    10381 ttggccaaat tcctggcagg tatcaagcag gactaggatt gtcagggatc tttcagttgt
    10441 gttcaatcgt tcgttaggaa ataggggcaa ttttgaattg cccctattct tataggaacg
    10501 attccgtgcg ataataggag caacaagttt atttgctgca atcgtgaagg aggtattctg
    10561 gtggaagaaa agacaatgaa agccttggtt tatcacgcgc cgcaccaatt cggcctggag
    10621 gatgtgccgg tgccgcaaat catggcgccg gatgatgcga ttatccgagt gacgctggcc
    10681 actccttgca catctgacat tcatatcctg gaagggcata tgccgtttgt caaagggccg
    10741 atcacccttg ggcatgagtt ttgcggagaa atcgtggaat taggtgcagc gattcaaggt
    10801 tatgaggtgg gtcagaaagt acactgcctg ccaggggcat tctgcaatca atgcgatatg
    10861 tgcaagagtg gttattccag tttttgtgag actggcggct gttatggaac ggtaaaaggg
    10921 ctgaatggag ccctggcgga gtatattcgc attccccatg cggatcaatc catgatcatc
    10981 atccccgacg gactgttgga agaagacgtt ttattgctgg gtgatatgct gggcaccggc
    11041 tatttcggcg tgaccaacgg cgaagtcaaa aaaggcgatg ccgtggcagt gtttggtgta
    11101 ggcccggtgg gcatgtgcgc ctgtatcctg gcgaaaaaag tgttcgaagc agaccccgtc
    11161 atcgcggtgg atctggccca atatcgcctg gatgccgtat taaaagcagg tgtagccgat
    11221 tatgttatta atcccaacga ggaagatgct gtcaagaaga ttcgcgaaat aaccggacgc
    11281 cgcggcgtga atgcagccat cgaaaccgcc ggggtacaga gcaccatgac cgcggcggcc
    11341 aaatcgctgg ccattggcgg gattttatct accattgcga tcttcgccaa accagtggaa
    11401 ttgccgctgc atatcatgcc caaccgcaac aaccaactga gaacgggcat ccagcaatgc
    11461 gccggtatgg atgtcatgct ggaggagatc aaagccggcc ggatcaatac caggtttatg
    11521 cagactcacc gttccccgtt aaatgatatc atccacgcgc tggatatttt cggcggcaac
    11581 aaagacggct gcatcaagta tttgattaca ccttttgaaa gatagctttt attgaaaaaa
    11641 gaacgcaaag ggcagttttg ccggaccggg caggactgtc ctttagttta tccggtggtt
    11701 tttgatctgg ccgaaacaac aggatggttt gcgggttgat gagcggttat ttttgctata
    11761 atgaattaca agcaaacaaa cgacggtcgg accatgataa aggagcaagc ggatgaaaga
    11821 gatcaattcc caggcacagc cattttattt cacgggaaat tcccaacagg cattattgtt
    11881 tctgcatgga ttcactgcct ccccgtcaga agtcagggtg gtggcggaaa aaatacatca
    11941 aagcattggc tgcagcgtaa gcggcatatt attgcccggg cacggcacga cgccggaaca
    12001 gctggatgcg gttcgctggc cggaatggta ccaagcggtc gagacggaaa tcaaatcatt
    12061 gcaggctagc tttcagcagg tctttgtcgg cggcttgtcc atgggcgggc tgctggcctt
    12121 gcatgccggc gtcaacctgt cgggtctggc gggggtcgtg accatcaacg ctccaattta
    12181 ttaccactat gcagtcatcc ccctggtctc ggatttaaca ggcttaatta ccccgttcta
    12241 tcacaaaaaa ggactggcgg aaattaaaaa acttgaaaac caagggcgct ttgcgtatcg
    12301 agtcatgccg ctgaaagcgt ttcacagcct caatcatctt cgcaagatcg tcatgcagga
    12361 ggtaaaggag ctaaagatcc ccgccttgat cgtgcaatcc atgctggacg aatcagtgca
    12421 ttcccgcagc gccgaatacc tatatgaaaa gacccgccca caaggggcac ggacgctgct
    12481 gctgagccaa tcacggcaca ttgccaccat ggggccgcag caggaaataa tcgttcagga
    12541 aatcgtgagt ttcatggaag accagcgttg tttcctggga aaatgagata aaaattctga
    12601 tagcatggtc tgtcatatta tcaacatgga tttaggag
//
LOCUS       DAOTHZ010000062        12592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_5970_length_12592_cov_7.501476, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000062 DAOTHZ010000000
VERSION     DAOTHZ010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 12592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12592
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5970_length_12592_cov_7.501476"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="P5549_07760"
     CDS             <1..189
                     /locus_tag="P5549_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13006.1"
                     /translation="NHALELYTDHRLLEELNHCTPEMQSAAVGMVQCILQTVQSFADG
                     AEQADDITMLCLKYQGSV"
     gene            249..1877
                     /locus_tag="P5549_07765"
     CDS             249..1877
                     /locus_tag="P5549_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HRW13007.1"
                     /translation="MAGMNDKREFALEIKNIHSAVKHIRELLNQYKCTDRDIVRAQLF
                     AEETVLYWAEQASTKDSFQIGLRKRFKTISLSLSYQGLPSNPLDLSMEAEDDAEFRFI
                     GQNILIGLSNTSYRYENGHNVVTFTLREQGINPLVALALALSGAIICGLAANQLAPSL
                     GASLGTAILTPLSNAFFGLLNAIVIPFLFVSVIASIFNMENLAQMKRIFRILFGWFLG
                     FTVLAATISVEAGLLFFPLQSAGAAGVGGAGIWTQITAMVFDMVPSNIFKSFLEGNTL
                     QTIFLAVLTGITMLIYKGRFPVITSVITESNLILSTLLDAICSLMPWVIFICIFNMLL
                     SGDGKALLDSIGAVAVICLCFLILIIIGLLSVVVIEKQNPFKYLQTIAPVLMIAFSTA
                     SSSATLAPHTMTAIAKQGIRDYLVNFSIPMGALFAKPFVVVTLFLMTLFMGHFYGVAF
                     SSADVVSMILLVTIMAIAVPPTLGMGTFLFTIMFKRFGIPLEGLAMATSLFMLLDYLI
                     TTGDIFSINVSMLHTEHQLRQIDKKAAASFTGAG"
     gene            2492..3283
                     /gene="uppP"
                     /locus_tag="P5549_07770"
     CDS             2492..3283
                     /gene="uppP"
                     /locus_tag="P5549_07770"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HRW13008.1"
                     /translation="MTLFQSIIMGLVQGLGEFLPISSSAHLVLVPWLFDWHDPGLTFD
                     VALHLGTLFAVIIYFWKDWLNLISAGLTKVKSSDGRLFWYLVAACIPGGVIGFLLESY
                     AETVFRTPLLIAVMLIVMGVILYWADQKSRKNNDIDHITFSTAMWIGVSQALAIIPGV
                     SRSGITITVGLLAGLTRESAARFSFLLSTPIIFGAALVKMPDVMANPAMINVNFVVGM
                     IVSAIVGLASIYVLLRYVQSKSYLPFAWYRFALGAVVIAVVFMRG"
     gene            3320..3760
                     /locus_tag="P5549_07775"
     CDS             3320..3760
                     /locus_tag="P5549_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRW13009.1"
                     /translation="MSKIDDALRYFADSGSCSQAIVLSFGPELGLPPEIAIKTAAAFG
                     GGIARSGELCGALSGALMVIGLTRVATPGKEHNEVIYELGQEFLQRFHNRIGSTVCRD
                     LLGADLSTAEGRQKARREDLFVLRCTNYVKAAAEILEEMLPVIR"
     gene            3850..5547
                     /locus_tag="P5549_07780"
     CDS             3850..5547
                     /locus_tag="P5549_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRW13010.1"
                     /translation="MKQYGKYFVGLLLIAAILSSLWCTGCSVREVFEGNKGQESFSFI
                     YMGDSQADPETGDYSIWGQMLSQAAADESRPAFVMIGGDLVNDGSSQAEWDRFLAAAE
                     PVRKKLPIYPAMGNHDNTALFKTIFALPENGPEGRQEAFYSFDYGDAHFTVMDSNVMG
                     AANQEDIKWLEQDLSQTDKTYKIVMFHHPAYPAIEIPKDSQRAEVIRKNFVPVMERGG
                     VDLVLGGHQHVYMRTHPLRDGQRSENGIVYLMGYSGGKQYSPGAYDYIACSVGGKPVY
                     SIISVDAQGITIKTMDRSGQVLDFTGAPDISAEQQDWTITIKGDGIQGEKKFTLAELA
                     AQPEAGFQHVYSTINNWPTARFYAVRGLTVRSILQAAGVLDTARAITFRSDDGYEMSF
                     TREQLLDTPHYYYPRVKEGSPEGAQPVEPVIAYEYKEGSADMGAAAADAPCLIIGQRN
                     LLEHTNPAFVVNVAEIIVSSRDPGQWEAATTFPTEGRIAAGESVKLQHKFSGLVKLHY
                     TLDGTDPTELSPMYNPSTYQPELNLPIPVREDTIIKVLVSGYGKQNSAISSFTFEVQ"
     gene            5568..7559
                     /locus_tag="P5549_07785"
     CDS             5568..7559
                     /locus_tag="P5549_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRW13011.1"
                     /translation="MQHLSKSKAMMAAFLMLFMVLILAGCGSKDSGDPQYEARAIAIE
                     GLNPSGKADVSISEISVAELRKLPQHELDASYKRTTGLFEEFKMSGPYLTDVIKQLGG
                     NLKDYAGIGVLGSDGYYCLLSREIIDATPDLMVAVAIDGQAKLDEDNAPARLAVQGQF
                     GPYWVKRIEKIILYKQIPEKAITSVWVFNYLAQGIEPYPYEYYGSKDDAIDLEQIFSR
                     LDHVDSKAFFTMKSADGFKKNEVLNMVKSRYYLKVEGKDAPTNVSPYIKLGMNVQNIA
                     WVSTNADAAIFPDKMMEYMDTRVIDGQKGITLEEVLYETEMKKVKSETFDILGTKGEK
                     ITVSGQDLSRGILVQRPDGSAGVVWEKSTGYENVADLLRIRLVEKPDAQAETTRTGND
                     ETAGEQPGAPSNNGQTSFSLPEKSAADTILTISGDGIKKPVYLSLDDLKKMKSGYIEQ
                     TYSIVNNYPTRKFTVAKGIDLAYLLGQAGLKSTASSFLVEASDGYKVTLTRDQLLGKR
                     YRYPGLKSASIANAAEVKPMLAWASAEGQEMNKAKEEELRLTVGQRGLNDVNTAVSVQ
                     MVAKITVSSQDNGRWAKAAASLIDGKISLQHENLDQVKLYYTLDGSQPDEFSQVYNPS
                     TTYFQPDLIKPIPVSGSGTLKVKVIGYGKHDSEVLTYAY"
     gene            7549..8568
                     /locus_tag="P5549_07790"
     CDS             7549..8568
                     /locus_tag="P5549_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRW13012.1"
                     /translation="MPIKKLMALMIVILGVWLSGCGEPAEPTEAELAAFNDILSQQYT
                     SKDFKILTQEVMTGERQEKFQAVKKVFTTPAGDYAFVSKPVAYNGPVELAVIIDRESG
                     ITAGMKIVRHQETEHYVRDMNNRWFTERFAGKQNDPYLQPVHLDAVHDNEIIIITGAT
                     VTTEGITNGVNACLGVYREAELGQTAPAVPYMVKFEKEEGEGPEETGSLAIRAYGVVL
                     GEVSLEQIKAMPSVTRTMSIHSTSGTTSHKFKGTLLANVIAAVDPELLQKYQAVQPVG
                     VDDYMSNITMEEVLAENAVYLMYEDNGQPLLTKPGKQGAMRVVVLDDVFGQRFTNYMI
                     EVVLE"
     gene            8572..8841
                     /locus_tag="P5549_07795"
     CDS             8572..8841
                     /locus_tag="P5549_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HRW13013.1"
                     /translation="MKISRFKAIISTILIVSALFSLSTGAILYFLQYGMWLCFTRGFL
                     NDIHALSGLVMGIAIVVHFIVNRHLYAKEIKILFFRGKKQENQNK"
     regulatory      8923..9042
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9107..10120
                     /locus_tag="P5549_07800"
     CDS             9107..10120
                     /locus_tag="P5549_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRW13014.1"
                     /translation="MPRLRSIIVIFLMLLVIVLSGCTKQEQVKYDRDFFAMDTYITCQ
                     VLSADEEQAAKALDAVQAAFMEIDRLTNRFAQDSEITAVNRSAGVAPIKVSADVFTMV
                     DTSLQWSDQSEGAFNILIGDVMDLWGFGSDHPTVPSQEALEAALTKTDYHQIVLDKAN
                     STIYLPEQGMVMDLGGVAKGYATDKAVAALEELGIDNALINAGGNVYALGRRADGTPW
                     KVGVQDPQNPQGIKTVLQGRDTALVSSGDYQRYFEKDGVRYHHILDPASGTPARASAG
                     TTVIMQSATLADILSTTLFVKGPLEGIKLAERLEEVEAALIITRDGKIYTTQNLNEYL
                     AED"
     gene            10227..>12592
                     /locus_tag="P5549_07805"
     CDS             10227..>12592
                     /locus_tag="P5549_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13015.1"
                     /translation="MNKQPGSQKKTGRFPRQIIAMILAVALMASVVPFYPGMNAAAAD
                     AFAGKTVQPVVVLSGQGIIADSTYTADNISNEKSYTMEELQDLEEITQLYSAINTTPT
                     SSIFLGKGISLEKLLQESNVASADYANYEIDVVAADGYTVRFDPAKTGQSATKGKPLK
                     TPALSVDRYYYSNLKDLTVGLDGESQYTYSNEAAAANGGSLAKTILAWERGGDRGFPE
                     TIPTTTAALNADEKPLLLMVGQQNVWEQNHPLFNKTVKKLLVGPAVTSTPITIDGVSM
                     TRNEILMMERADRSYTYSSSGGDKTDYVRGVPLSVLLNGYGENDSVSFTAADGYDMSS
                     ATVILGDAIANNYMLAYEKGTSIDDLAGIYSTAKKNASIHGYFTLYGDGQKPAQMIDS
                     ITVTPASGIDFATSPYRHITNGGISGQDGPYDIDAITGATLTIEGPGVTKSVPLPIRE
                     LEGQNAGAHRGTYTDKRSGTDWTLQYEGIKLSYIVNNMTSGDNGIHKTANAFKVLLKN
                     RVRQTIAEFTLDEIAAADDAGKPIIIAYGSGTTDNATSAPFVYDGATGIKDTLDNDDG
                     PIKLVYDKSVFAADPNPHYTEFGNVAYIYVAEQIAPGYKHNVAPYNTAENSQYVLTVT
                     GDKIGREVNYTVDQLEAMVAYDTEGKPMADGMGYRDEYSLANSSYWYVNEYEGVQLWK
                     LLQKSGLTADTALGEAKDTLVSFTATDNYKDFDKFTIEQVSNPNLFGYYEKNPADLND
                     GTYVSNDAEDLRGTGYPVLVAYGVNAYPYVIKNTLDGYMSGLSNDGGPL"
BASE COUNT         3247 a         2873 c         3307 g         3165 t
ORIGIN      
        1 aatcatgcgt tggaattata cactgaccat cgcttgctgg aggaattgaa tcactgtacg
       61 ccagagatgc aatcagctgc tgtcgggatg gtccagtgca ttcttcagac tgtgcaaagc
      121 tttgctgatg gtgctgaaca ggccgacgat atcaccatgc tttgtttaaa atatcagggc
      181 tcggtttaac ttgaatattt tgcccgggaa gcagttggag gagcataaac catatcaaat
      241 ggaggcgaat ggcaggaatg aatgataaaa gagagtttgc actggagata aaaaatatcc
      301 acagcgcggt gaagcatatt cgggagctgc tcaatcaata taaatgtacg gatcgagata
      361 ttgttcgggc gcagcttttc gcagaagaaa ccgttttgta ctgggcggag caagccagta
      421 ccaaagatag ttttcaaatc ggcctgcgca agcgcttcaa aaccatttct ttgtcattat
      481 cctatcaagg attgccatcc aaccctctcg atttgtctat ggaagcagag gatgatgctg
      541 aattcagatt tatcggccag aatatattaa tcgggctttc caatacaagc tatcgctatg
      601 aaaacgggca caatgtcgtt acctttacgc ttagggagca ggggatcaat cccctcgttg
      661 ctttggcttt ggctctgtct ggcgcgatta tctgcggttt agccgcaaac caactcgctc
      721 cgtcgctggg agcaagcctg ggtaccgcca tcctaacccc cttgagtaat gcattttttg
      781 gcttgctcaa tgccatcgta attccttttc tctttgtatc ggtgattgcc agtattttta
      841 atatggaaaa tctagctcag atgaaacgga tatttcggat tttgtttggc tggtttttag
      901 gatttaccgt tttggcggct accatttcag tagaggctgg attactcttt tttccgctgc
      961 aaagtgctgg tgctgctggc gtggggggcg caggcatctg gactcaaatc acggcgatgg
     1021 tgtttgatat ggtaccttcc aacattttta aatcttttct cgaggggaat actttgcaaa
     1081 ctatctttct ggctgttctg accggtatta ccatgctgat ctataaaggc cgctttcctg
     1141 tcattacttc tgtgataacc gagtccaatt taattttatc aaccttgttg gatgctattt
     1201 gctcgttgat gccctgggtg atctttattt gcatattcaa catgctgtta tccggtgacg
     1261 gcaaggcctt gcttgactca atcggggcgg tggcagtcat ttgcctgtgc tttttgattc
     1321 taatcatcat tggcttgtta agcgtggttg tgattgaaaa gcaaaatcct tttaaatatc
     1381 tgcaaaccat cgccccggtg ctgatgattg ctttttccac cgccagctcc agcgccacat
     1441 tggcgcctca taccatgact gccatagcca aacaaggcat ccgtgactat ctggtcaact
     1501 tttccattcc gatgggtgct ctttttgcca agccatttgt ggtggtgacc ttgttcctga
     1561 tgaccttgtt catgggtcat ttttatgggg ttgctttcag ttcagcggat gtagtgtcca
     1621 tgattttgct ggtgaccatt atggcaattg cggttccgcc gacgctggga atgggaacct
     1681 ttctttttac cattatgttt aaacgctttg gcataccgct ggaaggactg gcgatggcta
     1741 ccagcctgtt tatgctgctt gattatctga tcaccaccgg cgatattttt tcgatcaacg
     1801 tcagtatgct tcacacggaa caccaactga ggcagatcga taaaaaagcg gcagcatcat
     1861 ttacaggtgc aggataagcg aatcatggat gagaaatttg ggaggcagaa actcgtcaat
     1921 aaatgtgtgg atgacaaaat aaagaggcta tccgttaagg acagcctctt taaatttatt
     1981 aggggtggcg gagaaagaaa agggattctt ttaacaaaag accaataacc ttttaacctt
     2041 tcttgattgg ctagaaaccg aacctaccac ctgttatctt taccttgttt tacctgggtg
     2101 tttaaagaat taacttatcc ggataaccat ttgtttaact gatgatttga actttgaaat
     2161 acctgggtct caactgtata ttcttttttc agtggatttg aaaccgacga accaaataaa
     2221 ctgtttattt tctccgccac cttaatcgta ttatatgtaa taaatttagg tttggcaagg
     2281 ggaaaaacct ggtaaattaa ccgggcgatg atcttcgcag ctttatctgc gggctgttct
     2341 gtgaataagc taaagggcca tcctgctgat tccttcagga attaacatat tcttaatatc
     2401 tccttaagaa tccatgaaaa acatgttttt actggcgttt atgaatataa tagtgtttaa
     2461 tatgaaagta cataatggga ggtaggttaa cttgacttta tttcagtcga ttatcatggg
     2521 tttggtccag ggattgggag aatttctgcc tatatcgagc tctgcgcatc tggtgctggt
     2581 gccgtggctg tttgattggc atgatccggg tttgactttt gacgtggcac tgcacttggg
     2641 aactctgttt gcagtgatta tttacttctg gaaggattgg ctcaatctga tcagtgccgg
     2701 gctgaccaag gtgaaaagca gtgacggacg acttttctgg tatctggtag cggcctgtat
     2761 ccctggaggg gtaattggat tcttgctgga aagctatgct gaaacggtat tcagaacccc
     2821 gctgctgatc gcggtcatgt tgattgttat gggggtaatt ctctattggg cagatcaaaa
     2881 gagccgcaaa aacaatgata ttgaccatat taccttctct actgccatgt ggatcggagt
     2941 ttcccaggcg ctggcgatta taccgggagt atcccgttcg gggatcacta ttacggtagg
     3001 attgttggcc ggcttgacca gggagagcgc agcccgcttc tctttcttgc tatccacgcc
     3061 tatcatcttc ggcgctgcgc tggtgaaaat gccggatgta atggccaacc ccgccatgat
     3121 caatgtaaac tttgtggtcg gcatgatcgt ttcggcgatc gtcggcctgg ccagcatcta
     3181 tgtgctgctc aggtatgtgc agagcaaaag ctatttgcct ttcgcatggt atcgctttgc
     3241 acttggggcc gtcgttattg cggtcgtttt catgcgcgga taatgctgcg ccgttattgt
     3301 tttataagag agggacccta tgagtaagat tgatgatgca ttgcgatatt ttgctgacag
     3361 tggatcatgc tcccaggcca ttgtcttgag ttttggacct gagctgggat tgccgccgga
     3421 aatcgcgata aaaacggcgg cggcttttgg cggcggcata gcccgcagcg gtgaactctg
     3481 cggagcttta agcggtgctt tgatggtaat tggtttgacg cgtgtggcga caccaggcaa
     3541 agagcataat gaggtaatct atgaactggg tcaagaattt ctgcagcgtt tccataatcg
     3601 catcggctca accgtctgcc gtgatttgct aggagccgat ttgagtacag ccgagggccg
     3661 tcagaaagcc agacgtgagg atttgtttgt cctgcgctgc accaattacg tgaaagcagc
     3721 cgcggaaata ctggaagaaa tgttgcctgt catcaggtga cagtttggtg taaacagatt
     3781 atgataagag tgataaagca agcatggatg tttgaaacat catcattata accgggcggg
     3841 gtagaaataa tgaaacaata tgggaagtat tttgttgggt tgttattgat tgcagccatt
     3901 ttgtcaagct tgtggtgtac cggatgttct gtaagggaag tatttgaagg taataagggg
     3961 caagagagct tttcctttat ttatatggga gattcccagg ctgatccgga gaccggtgac
     4021 tattcgattt gggggcaaat gctgagccag gctgctgctg acgagagccg tccggctttt
     4081 gttatgatcg gcggagattt ggttaacgac ggcagcagtc aggcggaatg ggatcgtttc
     4141 ctggccgcag ccgagccggt acggaaaaag ctgcctatct atcctgccat gggcaaccat
     4201 gacaataccg ctctgttcaa aacgattttt gcgttgccgg aaaacggccc cgaaggccgg
     4261 caggaagcgt tttactcctt cgattacggg gatgctcatt ttaccgttat ggacagcaat
     4321 gtgatggggg cagccaacca ggaggatatc aaatggttgg aacaagacct gtcccaaacg
     4381 gataaaacct acaagatcgt tatgtttcat catcccgctt atccggcgat cgaaatcccc
     4441 aaagacagcc agcgggccga ggtcatccgc aaaaactttg ttcccgttat ggaaaggggc
     4501 ggagttgact tggtgctggg cgggcatcag cacgtctata tgcgcacgca tccgctgcga
     4561 gacggacagc gcagtgaaaa cggcattgtc tacctgatgg gctattcggg cggcaaacag
     4621 tattcccccg gagcttatga ttacattgcc tgcagcgtgg ggggcaagcc ggtctattcc
     4681 attatttcgg tcgatgccca aggtattacc atcaaaacca tggatcgcag cggtcaggtc
     4741 ctcgatttta ccggggcacc cgacatctct gcggagcagc aggattggac catcaccata
     4801 aagggggatg gcattcaggg agaaaagaaa tttaccctgg cagaactggc ggcccagcca
     4861 gaggccggct ttcagcatgt ctactccacc atcaacaatt ggcccacggc caggttctat
     4921 gccgtccgcg gcctaaccgt gcgcagcatc ctgcaggctg ccggggtgct ggatactgcc
     4981 cgggcgatta cttttcgttc ggatgatggc tatgaaatga gttttacccg ggagcaattg
     5041 ctggatactc cccactatta ttatccccgg gtcaaagaag gctcgccgga aggcgcccaa
     5101 ccggtcgagc cggtgattgc ctatgaatac aaggaaggct ctgccgatat gggggcagcg
     5161 gcagcggatg caccctgtct gatcattggc cagcgcaatc ttctggaaca taccaatccg
     5221 gcttttgtgg tcaatgtagc agaaatcatt gtatccagcc gggatccggg ccaatgggaa
     5281 gcggccacca cctttccgac agaaggcagg atagccgccg gagaatcagt caagctgcag
     5341 cacaagtttt caggtctggt caagctgcat tatacactgg acggaactga tcccacagag
     5401 ctttcaccga tgtataatcc cagcacctat cagccggagc tcaatcttcc catccccgtc
     5461 agggaggata cgatcatcaa ggtactggtg tccggctatg gcaaacaaaa cagtgcaatc
     5521 agcagtttta cctttgaagt acaataatac aaaacaggag gcacggcatg cagcatttat
     5581 cgaaaagcaa ggcaatgatg gcagcatttt tgatgctttt catggttctc atcctggccg
     5641 gatgcggcag caaggacagc ggtgatccgc aatatgaagc ccgggccatt gccatcgaag
     5701 gattgaatcc aagcggaaag gcggatgttt ccatctccga gatttcggtg gcggaactgc
     5761 gcaaattgcc tcagcacgag ctggatgcca gctacaaaag aaccaccgga ctgtttgaag
     5821 aattcaaaat gtcgggtccc tatctgacgg atgtgatcaa gcaactgggc gggaacctca
     5881 aggattatgc cggcattggt gtattgggca gcgacggtta ttactgcctg ctctccaggg
     5941 aaattatcga tgcgacccct gatttgatgg tggccgtcgc cattgacggc caggccaaat
     6001 tggatgagga taatgccccc gcccgtctgg cggtgcaagg tcaattcgga ccttattggg
     6061 taaagcggat tgaaaaaatc atcctctata aacagattcc cgagaaggcc attacttcgg
     6121 tatgggtttt taactatctg gcccagggaa tcgaacccta tccatacgag tattacggca
     6181 gcaaggacga cgccattgat ctcgagcaga tcttctcccg gctcgatcac gtcgacagca
     6241 aagccttttt taccatgaaa tcagccgacg gtttcaagaa aaatgaagtc ctgaacatgg
     6301 tcaagtcgag atattacctc aaggtggagg ggaaagacgc gcccaccaat gtatcgcctt
     6361 atatcaagct ggggatgaac gtgcagaata tcgcctgggt atcgaccaat gcggacgcgg
     6421 caattttccc cgacaagatg atggaataca tggacacccg ggtgattgac ggacaaaaag
     6481 gcatcaccct ggaggaagtt ttatatgaaa ccgaaatgaa aaaagtaaaa tcagaaacct
     6541 ttgatattct gggtaccaaa ggggaaaaga tcaccgtgtc cgggcaggat ctgagccggg
     6601 gcatcctggt gcaacgcccg gacggcagtg ccggcgtggt ttgggaaaaa tccaccggct
     6661 atgaaaacgt tgccgatctg ctgcgaatca gactggtaga aaagccggat gctcaagcag
     6721 agaccacccg gacagggaat gacgaaaccg ctggagaaca gccgggggca ccatcaaaca
     6781 acggccagac cagtttttct ctgccagaaa agtctgcggc cgataccatc ctgaccatca
     6841 gcggtgacgg tattaaaaaa ccggtctatc tttccctgga tgatttaaag aagatgaaat
     6901 ccggctatat cgaacagact tattccattg tcaacaatta tcccacccgt aaattcaccg
     6961 tggccaaggg gatcgatctg gcgtatctgc tgggacaggc tgggcttaag agcaccgcca
     7021 gcagctttct ggttgaagcc agtgacggct acaaagttac cctgacccgc gatcaattgc
     7081 tgggaaaaag gtatcgctat ccgggactga aatctgccag cattgccaat gcagcggaag
     7141 ttaaacccat gctggcttgg gcttctgccg aaggccagga gatgaacaaa gccaaggaag
     7201 aagaactgcg tctgaccgtc ggccagcgtg gcttgaatga tgtcaatacg gcggtttccg
     7261 tacaaatggt ggccaagatt acagtttcct cccaggacaa cggccgctgg gccaaggcgg
     7321 ctgcatcttt gatagacggg aagatcagct tgcaacatga aaaccttgat caggtcaaac
     7381 tgtattatac cctcgacggc agccaacccg acgaatttag tcaggtatat aaccccagca
     7441 ccacctattt tcagcccgat ctgatcaagc ccatcccggt cagtggttcc gggactttga
     7501 aggtaaaagt gattggctac gggaaacatg acagtgaggt cctgacctat gcctattaaa
     7561 aaattgatgg cgctgatgat tgttatattg ggcgtttggc tgtccggatg tggggaaccc
     7621 gcagagccga cggaagccga gctggcagcc tttaatgata tcctctcgca gcaatacaca
     7681 agcaaggatt tcaagattct cacccaggaa gtcatgaccg gcgaacggca ggaaaaattc
     7741 caagcggtca aaaaagtttt caccacgcct gcgggggatt atgcttttgt cagtaaaccg
     7801 gtcgcctaca acggaccggt cgagctggcg gtcattatag atcgggaaag cggtattacg
     7861 gccggcatga agatcgtccg gcaccaggag actgagcact atgtgcggga catgaacaac
     7921 cgctggttta ccgagcgctt tgccggcaag cagaatgatc cttatctgca accggtccat
     7981 ctggacgcgg tgcacgacaa tgaaattatt atcattaccg gcgccacggt gaccaccgaa
     8041 ggaatcacca acggggtcaa tgcctgtctg ggcgtatacc gggaagcgga gctcgggcaa
     8101 acagctcccg ccgttcccta catggtcaag tttgaaaaag aggaaggcga gggtccggaa
     8161 gaaaccggtt cactggccat tcgcgcctat ggggtggtat tgggggaagt cagcctggaa
     8221 caaatcaaag ccatgcccag cgttacccgc accatgagca ttcattccac ctccggcacc
     8281 acttcgcata aattcaaggg aaccctgctg gccaatgtta tcgcagccgt tgacccggag
     8341 ctgctgcaga aataccaggc ggtgcagccg gtgggcgtcg atgattacat gtccaacatt
     8401 accatggagg aagtgctggc ggagaacgct gtctatttga tgtacgaaga caacggtcag
     8461 ccgctactga ccaagccagg caagcaaggc gccatgcggg tggtcgtcct ggatgatgtg
     8521 ttcggacagc gttttaccaa ttatatgatt gaagttgtat tggagtaagg tatgaagatc
     8581 agccgattca aagccattat ttccaccata ttgattgttt ccgcgctgtt ctccctgagt
     8641 accggagcaa ttttgtattt tctgcaatac ggtatgtggc tgtgttttac ccgggggttt
     8701 ttaaacgata ttcatgcgct cagcggcttg gtgatgggga ttgccattgt tgtgcatttc
     8761 atcgtcaacc gacatcttta tgcaaaagag ataaaaattt tatttttccg cggaaaaaaa
     8821 caggaaaatc aaaacaaatg atagaaatat atagtaaacg gaattatccg gtgaagaata
     8881 tcttttaata aagtaataat gtgtttgatt taaaaatgaa taaatgcctt agtattccga
     8941 gtttattgct ctaacgtcct ttggaccatg acaatgaacc aatgggtacg gctggaaacg
     9001 gctgtgcctc ccgttttgga aaggaatcgc gcaatacatt tgcatacttt ctgataacgg
     9061 gcgttcatcg cctgtttttt attgcccaaa acgggaggga ttgagcatgc caaggttacg
     9121 atctatcatt gttatttttc taatgttgct ggtgattgtt ctgtccggct gtacaaagca
     9181 ggaacaggta aaatacgacc gggatttttt tgccatggat acctatatta cctgccaggt
     9241 cctgtcagcg gatgaggaac aggcggcaaa ggcattggat gcagtccagg ctgcttttat
     9301 ggagatcgac cggctgacca accgatttgc gcaggattcg gaaattactg cggtaaatcg
     9361 cagcgccgga gtagctccga taaaagtcag tgcggacgtt tttaccatgg tggatacttc
     9421 gctccaatgg tcggaccaga gcgagggagc ttttaatatt ctgattggcg atgtcatgga
     9481 tttatgggga ttcggttcgg atcatccgac tgtcccatcg caagaagctc tggaggcagc
     9541 gttgaccaag acagattacc atcagattgt gctcgacaaa gccaattcaa ccatctatct
     9601 gccggaacag ggcatggtca tggacctggg cggtgtggcc aaaggttatg ccaccgataa
     9661 agcggtggca gctttagaag aattgggcat tgataacgct ctgattaatg ccggaggcaa
     9721 tgtttatgcc ctgggaaggc gggccgacgg tacaccatgg aaggtagggg tacaggatcc
     9781 tcaaaatcct cagggaatta aaaccgtgct gcaagggcgt gatacggcct tggtctcttc
     9841 cggcgattac cagcgttatt ttgaaaagga cggggtgcgc taccatcaca tcctcgatcc
     9901 ggccagcgga actccggcgc gggccagtgc tggtacgacg gtcatcatgc aatcggctac
     9961 gctggccgat atcctttcca caacgctctt tgtcaaagga ccgttggagg ggattaaact
    10021 ggcggaaagg ctggaggaag ttgaagcggc tctgattatt accagagatg ggaaaatcta
    10081 tacgactcaa aacttgaatg aatatttggc ggaagattag tttaatggtt tttctttttt
    10141 ccccagggaa ggctgattct tcagacctga tgccctttga tagaggatag ataaaatcaa
    10201 atgatagaaa gaaaaggagt ggaaggatga acaagcaacc aggctcgcag aaaaaaacgg
    10261 gcagatttcc gcggcagatc atagcaatga ttctggcggt tgccttgatg gcctcggtgg
    10321 tgccatttta tccgggcatg aacgctgccg ccgccgatgc ctttgctggc aaaaccgtac
    10381 agccggtcgt ggtactcagt ggtcagggga ttattgccga cagcacctat acggctgaca
    10441 acatcagcaa tgaaaaatcg tataccatgg aggagctgca ggatttggag gagatcaccc
    10501 agctctattc agccatcaac accaccccca ctagcagcat cttcctgggc aagggtatct
    10561 ctctggagaa gctgttgcaa gagtcaaatg ttgcatctgc cgattatgca aattatgaaa
    10621 ttgatgtagt ggctgcggac ggatatacag tcagatttga tcctgccaaa accggccagt
    10681 cagctaccaa aggcaaaccg ttaaaaaccc cggcgttgag tgtagataga tattattatt
    10741 ccaatctgaa agatctgacc gtgggattgg atggcgaaag ccaatacacc tacagcaacg
    10801 aagctgctgc agcgaacgga ggctcgctgg ccaagaccat tctagcttgg gaacgaggcg
    10861 gcgaccgagg attcccggaa acaattccga ccactacagc agcattgaac gcagatgaaa
    10921 agccattgct gttgatggtt ggtcagcaga atgtatggga acagaatcac cctctcttta
    10981 acaagaccgt caaaaagctg ctggttggtc ctgcagtgac gtcaaccccg atcacgattg
    11041 atggtgtgtc catgactaga aatgaaatcc tgatgatgga gcgtgctgat cgcagctata
    11101 catattcatc ctcaggcggc gacaagactg attatgttcg cggtgtgccg ctgtccgttt
    11161 tattgaatgg ttatggtgaa aatgattcag tcagttttac cgctgctgac ggttatgata
    11221 tgtcaagcgc cacagttatc ttgggggatg cgattgccaa taactacatg ctggcttatg
    11281 aaaaaggcac cagtattgat gatctggcag gcatttattc aactgcaaaa aagaatgcca
    11341 gcatccacgg ttattttact ctatatggcg acggccagaa gccggctcaa atgatcgaca
    11401 gcatcaccgt tactcctgct tcaggaattg attttgcgac cagtccttac cgccacatca
    11461 ccaatggagg aatcagcggg caggacggac cctatgatat cgacgccatt actggcgcta
    11521 ccctgaccat agaaggcccc ggggtaacga aaagcgtccc cctgcccatc agggaattgg
    11581 agggtcagaa tgctggtgct caccggggga cctacaccga caagcggagt ggaaccgact
    11641 ggacgctgca gtacgaaggt atcaaactat cctatatcgt caacaacatg accagtggcg
    11701 acaatggtat tcacaagacc gccaatgctt ttaaagtctt gctgaagaac cgggtgcgcc
    11761 agacgattgc tgaatttacc ctggatgaaa tagcggcagc tgatgatgcc ggcaaaccaa
    11821 tcattatcgc ttatggttcc ggtacaacgg ataatgcaac cagtgctccc tttgtgtatg
    11881 atggagcgac ggggataaag gatacactgg acaatgatga cggtccgatt aagctcgttt
    11941 atgacaaatc cgtttttgct gctgacccca acccgcatta tacggaattc ggcaatgtgg
    12001 cttatattta tgtggctgag cagattgctc ccggatacaa gcataatgtt gccccttaca
    12061 atacagccga gaattcccaa tatgttttaa ccgtgaccgg cgacaagatc ggccgggaag
    12121 tgaactatac ggtggatcag ctggaagcca tggtagctta tgatacagaa ggtaaaccaa
    12181 tggctgacgg catgggatac cgggatgaat acagtttggc caacagcagt tactggtatg
    12241 tcaatgaata cgaaggggtg cagctctgga aactgctgca gaagtcaggt ttaacagctg
    12301 atactgccct cggggaggcc aaggatacac ttgtatcgtt cacggctact gacaactata
    12361 aggatttcga caaatttacc atcgagcagg tatccaatcc caatctgttt ggctactatg
    12421 aaaagaaccc ggctgacctg aatgacggga cctatgtcag caacgatgcc gaggatttaa
    12481 ggggaaccgg ttatccggtg ttggttgcct atggcgtgaa tgcctatccc tatgttatta
    12541 agaacacgct ggatgggtac atgagcggtc tttccaatga cggcggcccg ct
//
LOCUS       DAOTHZ010000063        12036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_6310_length_12036_cov_6.444287, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000063 DAOTHZ010000000
VERSION     DAOTHZ010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 12036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12036
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6310_length_12036_cov_6.444287"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..886
                     /locus_tag="P5549_07810"
     CDS             383..886
                     /locus_tag="P5549_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRW13016.1"
                     /translation="MKEIYYKIGEVSEILKIEPYTLRYLESSLKLKIKRDERGERLYT
                     DADLDTLRLILQLKNEKGLNTTAIKLALDNLNESGQKDNQVPQVYSAADFVEIVDVTR
                     NILQQNEEILEQNRVMQQKLDALEKSQRTMDKQNSARWDELLEVLRAETNDKRSGWLA
                     KLMRTKS"
     gene            1082..2641
                     /locus_tag="P5549_07815"
     CDS             1082..2641
                     /locus_tag="P5549_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRW13017.1"
                     /translation="MVSVSVFLTLVPVMVVLILLVWVKWSADLSGFIGWILAVAIAFL
                     FFHTSLQVGLTASLAGIVASFPVSLMVLTSIFQITFLEATGALKRIVVFVKTLAKNDQ
                     VVQVMIINMGIGTLLVSVGATPVSILPPIMLALGYSTFVAVALPAIGFDSLCTYALLG
                     APLVVFADLTGTSLNDSALVFSQFMPIISTMIGFGMLLIIGGWSKVREGIVPCLIAGL
                     TMGLTAMLVAHIGKGIVLTGVIAGMATVLVMIVYLKLKGKPLLDRSCVTEQEKAVERN
                     MSLVKALSPWLILIGACLVINFWQPLFNLLFKDLAMPLNIIPGGKPINTRMLWNAYTW
                     VIVSTLLAVPFIKPSKAQWQEIMQKWWKRAPRPTFSAAIFFAIAYVMTYSGFAATPQG
                     WSLSVAENNMISVLSVSSANFFHSLYPAAAAFLGLLGGFVSGSEASTIALFTKYNMLT
                     SELLKVNPLVVTAGTAIAGGLASVISPAKLQNAAATIDSIGVESKVIKIVIPIALAFT
                     FVAAILTLLWA"
     gene            complement(2695..2841)
                     /locus_tag="P5549_07820"
     CDS             complement(2695..2841)
                     /locus_tag="P5549_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13018.1"
                     /translation="MNRSDLSMDDFEAAAAEKNKELLIIKAQIVLANLQQIEKSLAKS
                     MNMQ"
     gene            complement(2884..3207)
                     /locus_tag="P5549_07825"
     CDS             complement(2884..3207)
                     /locus_tag="P5549_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRW13019.1"
                     /translation="MIITTSQTVQNRSILNYLGLVSGESILGPKFMETVLNGVSSPQG
                     CTGACKPAVEEARQAALEEMKNKAVELGAEAIIAVDIDYVELKDQMLLIAVSGTAVKL
                     VEYRD"
     gene            3437..4684
                     /locus_tag="P5549_07830"
     CDS             3437..4684
                     /locus_tag="P5549_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13020.1"
                     /translation="MFDYLMTPQQIKLRDETRDLIKWIPREYILDMDADKIKYPKEFM
                     IEAGRRNLIGIRVPVEYGGRGGQWQDDIIVAEEVCVPGYIFGCAWGVTGDVVTEALVH
                     FGTEEQKKKYLPPICKGDKYAAECLTEPRGGSDFFGATTIARKDGSDFILNGQKRFIV
                     GGEGADYFLVYAKTDLEAPSAKSMTCFMVERDMGVQTEYLYNLMGSRGGGTARVVFDN
                     VRVPQENVIGEVNGAGTVFNQMMIPERFGTAAMTIGAARPALDIATDYSTKRKAFGTQ
                     IKNFEGVSFKIAEMVIKLDSARAMIYTTALAIDSGKVKPNRCRRMASESKKFVTEQCW
                     DVANLAMQVMGGIGYTDVYPIEKIVRDLRLAMIWVGTNEIMSLIIQHEHYRERLAELA
                     SGEKRDSEMDALNAFAESEKIFE"
     gene            4732..6045
                     /locus_tag="P5549_07835"
     CDS             4732..6045
                     /locus_tag="P5549_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13021.1"
                     /translation="MYKRKLMSAEDAVKVIANGDLIASASINGQPTQIVDALVRRMAS
                     GAIKDARFLFSLSVRMSSLHNPEIIEQCYQNNNTLDTMYAGPLERYFLDQGSYSYVPH
                     RLQDGPLMTARVGLHAGLITVSAMDKHGYFSTGTNPEYIYGFIRRNPGCKILAEVNQY
                     MPKTFGNNHFHISELAAIVENDVPLFELPPIPITEQDELIGQYIAERVPDGACLQLGI
                     GGMPNAVAKYLTHKKDLGIHTEMLCDTMVDLYEAGAITGARKKLLPRKWAACFAMGTK
                     RLYDFMADNPMVELYDSEWINDPYNIAKNDNVVSINATLEVDLSGQCASEAIGTRQYS
                     GAGGQMDFTEGTWRSNGGQAFLALYSTYTDKNGVKHSSIVPTLTPGAMVTTTRNDVQY
                     VVTEYGVAWLKGFDLRQRVKDLVRIAHPDFRDWLLEEARKLKYIR"
     gene            complement(6298..6384)
                     /locus_tag="P5549_07840"
     tRNA            complement(6298..6384)
                     /locus_tag="P5549_07840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6348..6350),aa:Leu,seq:cag)
     gene            complement(6434..7396)
                     /locus_tag="P5549_07845"
     CDS             complement(6434..7396)
                     /locus_tag="P5549_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461526.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRW13022.1"
                     /translation="MESKSIVPQWGEKRYNNINDDWRRRFGEKIGKVTLDAGFTCPNR
                     DGTLSDQGCRFCSPAGSGDFVALPGATLAQQYERGREVISNKWPVRKYIAYLQSFTNT
                     YAPAERLREIYQSVLSLPGVVGLAIATRPDCLPQAVLDLLAEINQQHYLLLELGLQTI
                     HDRSAAAMNLQYNFADFCTALEQLQVRQIDVCAHIILGLPGEDRQDMMATAQALAELP
                     LQGIKIQLLHLLRGTPLAEEYEEHPFPFLSQAEYVDLVTDIIGILPPSLVIHRLTGDG
                     PRHLLLGPRWSLNKRAVLNAIDQTLVQKSSWQGKFYAKNSPSDS"
     gene            7535..8062
                     /locus_tag="P5549_07850"
     CDS             7535..8062
                     /locus_tag="P5549_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13023.1"
                     /translation="MSQKSWKRFIVPSIFITVFLFAVVFTALQQRPVNDYPDTLTPLL
                     ASDFVITGSNPELRVGESDLGDVSLQFPGGNMLGRSGVYRPADEELLFTFTRKTNLLN
                     KMDILGPGMATARAVSVNDSFDQVLSQYGHGFVRSYYKSNPQIFDAIYGEEQCIVFHV
                     ENNVVKRIVVIHETD"
     gene            8105..9127
                     /locus_tag="P5549_07855"
     CDS             8105..9127
                     /locus_tag="P5549_07855"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRW13024.1"
                     /translation="MIQIEGLTKIYNAGGGEVLALDNVSLQVNKGEIFGIIGLSGAGK
                     STLIRCINMLEKPTAGTIRVDGQNISTLDKKDLREARQKIGMIFQQFNLLSSRTVFDN
                     ILFPLEIAGISPKQARKRVQDLLDLVGLNEKARVYPEQLSGGQKQRVGIARALANDPK
                     ILLSDEATSALDPQTTRSILKLLKDINRQLNLTILLITHDMNVIKETCDRVAVIDNSK
                     IVEIGDVLSIFSNPGTPTSKSFINTIINKEIPEELLHPSFIGNEQMTSKLIRASFIGA
                     SAGEPVISGLVQKYDIKANILYGNIDRVKDTPFGNLTLELIGDPALMGQALDYLRDNG
                     LEIEVL"
     gene            9195..9842
                     /locus_tag="P5549_07860"
     CDS             9195..9842
                     /locus_tag="P5549_07860"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HRW13025.1"
                     /translation="MLDKVTLATWETIYMVAISTAFSYVLGLPLAIILVITDKGHIME
                     NLGLNRTLGSIVNAARSIPFIIFLILLIPLTRIIVGTSIGTVASIVPLTLAAVPFVAR
                     LVETSLKEIDWGLIEAALSMGANTWQIITKVLIPEAFPAIILGVAITTINLIGYSAMA
                     GIVGGGGLGTLAYYYGYQRYQTGIMWVTVILLIILVQLIQMLGDKLSNKIIYKRR"
     gene            9847..10677
                     /locus_tag="P5549_07865"
     CDS             9847..10677
                     /locus_tag="P5549_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRW13026.1"
                     /translation="MKKKLLVLLTGLMLMSLVLAGCSGQKDAETPEDAGDAPAKVVVG
                     ATAKPHAEILEIVKPMLAKENVDLEIKVFTDYVQLNPALKDKQIDANFFQHIPYLEDY
                     NKNNNAELSWTVKVHNEPMGVYSEKVKDINEVADGAKVGIPNDATNGGRALMVLEKAG
                     LIKLTEGVGVNATDKDIIENSKKIEIKMMDAAMLPRSLADLDICVINSNYAIEAGLNP
                     VNDSIFIEPKDSPFANILAVRPEDKDKDTIKKLGAALQSPEVKKFLEDTYQGSCVPAF
                     "
     gene            complement(11188..11652)
                     /gene="ribE"
                     /locus_tag="P5549_07870"
     CDS             complement(11188..11652)
                     /gene="ribE"
                     /locus_tag="P5549_07870"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRW13027.1"
                     /translation="MAKILEGKLIGENQRFGIVVSRFNEFITNKLLSGCMDTLIRHGV
                     DQENIDIAWVPGAFEMPLAAQKMAAREYDAVICLGAVIRGATPHFEYVSAEVTKGIAQ
                     VGLNTGKPIIYGIITADTIEQAIERAGTKAGNKGADAAVTAIEMVDLLKNIG"
     gene            complement(11706..>12036)
                     /locus_tag="P5549_07875"
     CDS             complement(11706..>12036)
                     /locus_tag="P5549_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRW13028.1"
                     /translation="RQEGRGIGLVNKIRAYKLQDNGRDTVQANLDLGFPEDLRDYGIG
                     AQILVDLGVGKMRLLTNNPKKIKGLEGYGLEVVERVPIEMEIKPENARYMHTKRDKMG
                     HLLSGNK"
BASE COUNT         3075 a         2758 c         2895 g         3308 t
ORIGIN      
        1 aattgtcata aatatatatt gataaaaaac catctcgtga gaggtggttt ttttgcatat
       61 gaaaatcaaa tgatttgata agcacaaaaa caattaccga gctgattggc caaacgtttc
      121 aagccataaa aaaaagccgt ccaatcatgt atggacggct ttgctcatat ataaatacta
      181 acaggtttca tgtatatgat gagcaaaaaa gaggggaaag ttttgcctgt caatgagttt
      241 gtcaatgagg ttcataaatt cctgggttaa tacgctggct tgattcctgt ttgtgaagct
      301 gaaaaagcgg ctgcagattg ttctgttcac ttttgaccgg gctaataaca atttatgaag
      361 ccgggagagg aaaggttcca tcatgaagga aatttactat aaaattggtg aagtctcgga
      421 aatcctgaaa attgaaccat ataccctgcg ttatctggaa agcagcctga agctcaaaat
      481 taaaagagat gagcggggtg aacggctcta tacggatgct gatttggata ccttgcgatt
      541 aatcttgcag ttaaaaaatg aaaaagggct gaataccacc gctattaaac tggcgctgga
      601 taatttgaat gagtccggtc aaaaggataa tcaggtccct caggtttatt ctgctgctga
      661 ttttgtggaa atcgtcgatg taacccgcaa catcctgcag caaaatgaag aaattctcga
      721 acaaaaccgc gttatgcagc aaaaactgga cgccctggaa aagagccagc gtacaatgga
      781 taaacagaat tctgcccgct gggatgaact gctggaggtg ctgcgcgccg aaactaatga
      841 taaaaggtcc ggttggctgg ccaaactgat gcgtaccaaa tcataattgt tgcgctgtat
      901 ttttcccgga ttttgtttgt ttagtccctt gatctaataa tttaatagcc aaaattgatg
      961 catttatgat acgatattag aaatgtgcag tgaatgaaca tttcccctgt aggattgtag
     1021 tgtggcgttt cattgacagg cacgttacgg ctcaaaataa ttttatatgt agaatgggag
     1081 gatggtttcg gtgagtgttt ttttaaccct tgtgccggtc atggttgtgc tgattttact
     1141 ggtttgggta aaatggtcag cagacctcag tggttttatc ggatggattt tagcggttgc
     1201 gattgcattt cttttctttc acacctcgct gcaagtcggc ctgacggcca gtttggcagg
     1261 cattgtagca tcatttcctg tttccttgat ggttttgaca tcgatttttc aaattacctt
     1321 tcttgaagcc accggtgcgt taaaaagaat cgtggttttt gtaaaaaccc tggcgaagaa
     1381 cgatcaggtc gtgcaagtca tgattattaa tatgggtatc ggtacgctgc tggtatcggt
     1441 gggcgccacc ccggtatcaa ttcttccccc gattatgctc gcgctgggct actcaacttt
     1501 tgtggcagtg gctcttcctg cgattggttt tgattcgctt tgtacgtatg ccctgctggg
     1561 cgctccgctg gtggtgtttg ctgatttaac cggcacctct ttaaatgatt cggcgttggt
     1621 tttttcgcag tttatgccga tcatctccac catgataggc tttggcatgc ttttgattat
     1681 cggtggctgg agcaaggtca gggaaggcat cgtcccctgc ctgattgccg ggcttaccat
     1741 ggggttaacc gccatgctgg tcgcccatat cggcaaaggc atcgtcctga ccggtgttat
     1801 tgccgggatg gcaaccgtgc tggtcatgat tgtatacttg aaactcaagg gcaaaccttt
     1861 gttggatcgc tcttgtgtta ctgaacagga aaaagccgtc gagcgcaaca tgtctctggt
     1921 caaagcgctg tctccctggc tgattctcat cggcgcctgt ctggtgatta atttttggca
     1981 accgctcttt aatctcctct ttaaagatct ggccatgccg ctgaacatta tcccgggcgg
     2041 caagccgatt aacaccagaa tgctgtggaa cgcttatacc tgggtcattg tcagtacatt
     2101 gctggcggtt ccttttatca aacccagcaa ggcccaatgg caagagatta tgcaaaagtg
     2161 gtggaagcgc gcaccccgtc ccactttctc ggccgccatc ttttttgcca ttgcctatgt
     2221 gatgacctac tccggttttg ctgccacccc gcaaggatgg agcttgtcgg tggcagagaa
     2281 taacatgatc tcagtcttgt cggttagttc agccaacttt ttccacagcc tctaccccgc
     2341 cgcggctgct ttcctgggct tgctgggcgg attcgtgagc ggcagcgaag cctcgaccat
     2401 tgcgctgttc accaaataca atatgctgac ttctgaattg ctaaaagtca atccgttggt
     2461 ggtaaccgct ggtactgcaa tcgctggcgg tctggccagt gtgatttctc cggccaagct
     2521 gcaaaatgcg gcagctacca ttgacagtat tggggttgag tccaaggtca ttaaaatagt
     2581 cattcccatt gcccttgcct ttaccttcgt agccgctatc cttactttgc tctgggcctg
     2641 attaaattag aaaaaaagcc agaaatgttt cacgtgaaac atttctggct ttttttattg
     2701 catattcatt gattttgcca gggacttttc aatttgctgc agattggcta atacgatctg
     2761 cgctttgatg attaacagct ctttgttttt ttctgctgcg gctgcttcaa aatcatccat
     2821 cgacagatcg ctgcggttca caaaaaaaga ctgccgtagc ggcagtcttc aacaacgatt
     2881 gtatcaatct ctgtattcaa ccagttttac tgcggttccg ctgacggcaa tcaaaagcat
     2941 ttggtctttc agttctacat aatcaatatc aactgcgatg attgcttctg caccaagttc
     3001 aacagcctta ttcttcattt cttcaagggc tgcctgacga gcttcttcaa cagcaggttt
     3061 gcaggctccg gtacatcctt ggggactgct tactccgttt aaaacggttt ccataaactt
     3121 gggtccaagg atcgattcac cggaaaccag accaagataa ttgagaatgg atctgttttg
     3181 gacggtttgg ctggtcgtga ttatcatcga tcatcctcct ctctttcccg atcgtatttc
     3241 atcggatgaa acaagcaact actgcctttg tctttggaac tacagccttc ttctattatt
     3301 atgacaggca gcaaatattt tgtcgagttt ttgtcagaat tttctgatat tttttataaa
     3361 atattgtgat ttttcaggcc aaaagaggat tttttataac aatgcgtaat aaaaacagca
     3421 aggagagtga tgagacatgt ttgattattt aatgacgccg cagcaaatca aactgcggga
     3481 tgaaaccaga gatctcatta aatggattcc ccgcgaatat atcctggata tggatgccga
     3541 caagatcaag tatcccaaag aatttatgat cgaggccggg cggcgcaatt taatcgggat
     3601 cagggtaccg gtggaatatg gcggacgtgg ggggcaatgg caggatgaca tcatcgtggc
     3661 ggaagaggtc tgtgtccctg gttatatttt tggctgtgcc tggggcgtga ccggtgatgt
     3721 ggtcaccgaa gccctggttc attttggcac cgaggagcaa aagaaaaaat acctgccgcc
     3781 catctgcaaa ggagataaat acgctgccga atgtctgacc gagccacggg gcggttcaga
     3841 ttttttcggc gcaacgacca tcgcccggaa agacggttcg gattttattt tgaacggaca
     3901 aaaaagattt attgtcggcg gcgaaggagc agattatttt ctggtctatg ccaagaccga
     3961 tctggaagcg ccttcggcca aatcaatgac ttgttttatg gtggaacggg acatgggcgt
     4021 acaaaccgag tatctgtata atctcatggg ttcgcgcggc ggcggaaccg cccgagtggt
     4081 tttcgataat gtccgggtgc cgcaggaaaa tgttattggt gaagtcaacg gcgcgggcac
     4141 ggtctttaac cagatgatga tcccggaaag gtttggcaca gctgccatga cgattggcgc
     4201 cgcccgaccg gccttggata ttgcaacgga ttacagtacc aaacgcaagg cttttgggac
     4261 ccagattaaa aatttcgaag gggtcagttt taaaatcgcg gaaatggtga ttaaattgga
     4321 cagtgcgcgg gccatgattt atacgacagc gttggcgatc gactccggca aagttaaacc
     4381 caaccgctgc cgccgcatgg catcggaatc aaaaaaattc gtgaccgaac agtgttggga
     4441 cgttgccaat ctggccatgc aggtgatggg cgggattggc tatacggatg tctatcctat
     4501 tgagaaaatt gtgcgggatc tgcgtctggc gatgatctgg gtcggaacca acgaaattat
     4561 gagtttaatc attcagcatg aacattaccg ggaaagactg gccgaattag cttccggtga
     4621 aaaacgtgac agtgagatgg atgcactcaa tgcctttgct gaaagcgaga agatttttga
     4681 atagtgtttg tttaaaggga ggagattggg atggagagta aatagcagga aatgtacaaa
     4741 cgtaaactaa tgtctgcgga agacgctgtc aaagtaatcg caaatggtga tttgattgct
     4801 tccgcttcca ttaacggtca gccgacgcaa atcgtggatg cgttggtgcg gcggatggcc
     4861 agcggcgcaa tcaaggatgc ccgttttttg ttttcattgt cagtcagaat gtcttcttta
     4921 cataacccgg aaattatcga gcaatgctac cagaacaaca ataccctgga tacgatgtat
     4981 gccggtccgc tggaacgcta tttcctcgat caggggagtt attcctatgt cccccatcgt
     5041 ctccaggacg gccctttaat gaccgcccgg gtgggattgc atgcaggcct gatcactgtc
     5101 tcagccatgg ataaacatgg ttatttcagc accggaacca acccggaata tatttatggt
     5161 tttattcgcc gcaatcccgg ttgtaagatt ttggcggaag tcaatcaata tatgcccaaa
     5221 acgtttggca ataaccattt ccatatttca gagctggcag ccatagttga aaatgatgtg
     5281 cctttatttg aactgccgcc cattcccatc accgaacagg atgaattaat cggacagtat
     5341 atcgccgaaa gagtaccgga cggcgcctgc ctgcagttgg gcatcggcgg aatgcccaat
     5401 gcggtggcca agtatttgac ccataaaaaa gacctgggca tccacaccga gatgctgtgt
     5461 gatacgatgg ttgaccttta tgaagccggt gccatcaccg gtgcccgcaa aaaattgctg
     5521 ccgcgcaagt gggctgcctg ttttgccatg ggtaccaaac gtctgtatga ttttatggct
     5581 gataatccca tggtcgagct gtatgactcg gagtggatca atgatccgta taatattgcc
     5641 aaaaacgata atgtggtttc cattaatgct accctggaag ttgatctcag cgggcaatgc
     5701 gcttccgaag caatcggcac ccgtcaatat tccggtgccg gcggacaaat ggatttcacc
     5761 gaaggaacct ggcgctccaa tggcggacag gcattcctgg ctctgtactc cacgtataca
     5821 gataaaaacg gagtcaagca ttccagtatc gtgccgaccc taaccccggg ggccatggtc
     5881 accaccaccc gcaacgacgt gcagtacgtg gtcaccgagt atggcgtcgc ctggctgaaa
     5941 ggtttcgatt tacggcagcg ggtaaaggat ctggtgcgca ttgctcatcc tgatttccgc
     6001 gactggctgc tggaagaagc acgcaaactg aagtatattc gctagaaagg ctggatttaa
     6061 acgaaattgc aagggagggc agatagtctg tcctcctttt ttattataca ccggttaacc
     6121 gtggcggttt tctgcaacga tgacaacctt tatcggcgga aacaagcgaa acaaatccac
     6181 ttatactggc atttttcctg caacaatgtt tcacgtgaaa cattttctta ttcccgtctt
     6241 tgttcagtac cctctcagaa aacactaaag ggaggcctca acctcccgag aattcaatgg
     6301 tgcggtagag aggacttgaa cctccacgaa gttgccccca ctagaacctg aatctagcgc
     6361 gtctgccgat tccgccacta ccgcgtgctg tttgtctcag caagaatgat tatattacac
     6421 gaatgtcaga ctgtcaagaa tcgctgggcg aatttttcgc gtaaaatttc ccctgccagc
     6481 tgcttttttg caccaaagtc tggtcaatcg cgtttaagac agcacgtttg ttgagactcc
     6541 agcgggggcc aagcaacaaa tggcgggggc cgtcgccggt gaggcgatgt atcaccaggg
     6601 aaggcggcag gatgccgatg atatcggtga ccaggtctac atattccgcc tggcttaaaa
     6661 acgggaacgg gtgctcttca tactcctctg ccagaggtgt cccccgcaag agatggagaa
     6721 gctgtatctt tatgccttgc aggggcagtt ctgctaacgc ctgggcggtt gccatcatgt
     6781 cctgccgatc ctctccgggc aggcccagta taatatgggc gcatacatcg atttgacgga
     6841 cctgcagttg ctccagcgcc gtgcaaaaat cagcaaaatt atattgcaga ttcatggcag
     6901 cggcgctgcg gtcatgaatg gtttgcagac ccaattcaag cagcagataa tgctgctggt
     6961 tgatttccgc cagcaaatcc aggaccgctt gcggaaggca gtcaggtcgc gtggcaatag
     7021 ccagtccgac gacgcccggc agagataata cagattgata gatttcccgt aatcgttcgg
     7081 cgggtgcata ggtgttggta aaagactgca aataggcgat atatttccgg acaggccatt
     7141 tattgctgat gacttcccgg ccccgttcgt attgctgagc cagggttgca cccggcaggg
     7201 cgacaaaatc tcctgacccg gccggactgc aaaaccggca gccctggtca gacagggtgc
     7261 catcgcgatt gggacaggta aaaccggcgt ccagggttac cttgccgatt ttttcgccaa
     7321 agcgcctgcg ccaatcatca ttgatgttgt tgtaacgttt ctctccccat tggggcacaa
     7381 tactcttgct ttccatcgcc atacttcctt gacattcgtg taaaaaggcc tcggttttca
     7441 gcttattttt aatctcttgt ggtgctttta tgttatatta tataatggat tgtcaaaatc
     7501 agattagcat cgcaaaataa ggatgtgaat tattgtgtca cagaaatcct ggaaacgttt
     7561 tatcgtccct tccattttta ttacggtgtt tctgttcgcc gtagtattca cggccttgca
     7621 gcaacgtccc gtcaacgatt atcctgacac tttgacgccc ctgctggcca gtgattttgt
     7681 gattaccggc tcgaacccgg aactgcgcgt cggtgaatcc gatttgggag atgtctccct
     7741 acagtttcca ggtggaaata tgctggggcg cagcggtgtt taccggcctg ctgacgagga
     7801 acttttattt acctttaccc gcaagaccaa tttgctaaat aaaatggaca ttttaggccc
     7861 gggcatggcc actgcccgcg cggtatccgt aaatgacagc tttgaccagg tgctttccca
     7921 atatggccac ggttttgtgc gttcttatta taaaagtaat ccgcaaatct ttgatgccat
     7981 ttatggcgaa gaacaatgta tcgtttttca tgttgaaaac aatgtcgtta aaagaatcgt
     8041 cgtcattcat gaaaccgact agatcggttg atcagagcaa catgattttt aggaggcatt
     8101 ctgattgatc caaattgaag gtttaaccaa aatttataat gccggcggcg gtgaagtgct
     8161 ggccctggac aatgtcagcc tgcaggtcaa caagggtgag atatttggca ttatcggtct
     8221 cagtggagca ggcaaaagca cgttgatccg ctgcatcaat atgctggaaa aaccgacggc
     8281 cggaacgatt cgtgtagacg gacaaaatat cagcacgctt gataaaaaag atttgcggga
     8341 agcacggcaa aagatcggca tgatctttca gcagttcaat cttttgtcat cgcgcacagt
     8401 ctttgacaac attttgtttc cccttgaaat agctggcatt tctcccaagc aagcccgcaa
     8461 acgcgtgcaa gatttgctgg acctggtcgg cctcaatgaa aaggcccggg tctatcctga
     8521 acagctaagc ggcggacaaa aacaaagagt cggcattgcc cgggcattgg ccaacgatcc
     8581 caaaatactt ttgtcagacg aagccacttc ggcattggat ccgcagacga cccgctctat
     8641 cctgaaactt ttgaaggata tcaatcgcca attaaatctg accatcctgt tgattaccca
     8701 tgatatgaat gtcatcaagg aaacctgtga ccgggtggcg gtgattgaca acagtaaaat
     8761 cgtggagatt ggcgatgttc tcagtatctt ttccaatccc ggcaccccca cttccaagag
     8821 tttcatcaac accatcatca acaaggaaat cccggaagag cttttgcatc cttcttttat
     8881 cggcaatgag caaatgacca gcaaattaat ccgggcctca tttattggcg cttcggccgg
     8941 cgaaccggtg atttccggct tggtacaaaa atatgatatc aaagccaata ttttatatgg
     9001 caatatcgat cgggtcaagg acactccctt tggcaatctg acgctggaac tgatcggcga
     9061 tccggcttta atgggtcagg cgcttgatta tctgcgcgat aatggactgg aaatagaggt
     9121 gttgtagttt gttggaaata atctctggtt tcaacctgtc aaatatcagc aatattttgg
     9181 ctgagaatgc ctggatgttg gacaaagtca ccctggcgac ctgggaaacg atttacatgg
     9241 tcgccatttc aacagctttc agttacgtcc tggggcttcc cttggccatt attttagtca
     9301 ttactgacaa gggccatatc atggaaaacc tgggcctcaa ccgaacattg ggcagtatcg
     9361 tcaacgcagc ccgttctatt ccctttatca tatttctgat attactgatt cctcttaccc
     9421 ggatcatcgt cggtacatcc atcggtacag tagcctccat tgttcccctg accttggcgg
     9481 cggttccttt cgtggcccga ctggtggaaa cttctttaaa agaaatcgac tggggcttga
     9541 tcgaagcggc tttgtccatg ggcgccaata cctggcagat cattaccaag gtgttaatcc
     9601 cggaagcctt tcctgccatc attctggggg tggccatcac caccatcaat ctgattggtt
     9661 attccgccat ggcaggcatt gtcggcggcg gcggactagg caccctggcc tactattacg
     9721 gctatcagcg ttatcaaacc ggcatcatgt gggtgaccgt aatcttgttg attattttgg
     9781 tgcaactcat tcagatgctg ggcgataagc ttagcaataa aattatttat aagaggaggt
     9841 aaataagtga aaaagaaatt attggtactg ctcactggtc ttatgctgat gtctctggta
     9901 ctggctggct gctccggtca gaaggatgcg gaaacgcctg aggatgccgg ggatgctcca
     9961 gccaaagtcg tcgtgggggc gacggccaaa ccgcatgcgg aaatccttga aatcgtcaaa
    10021 ccaatgctgg ccaaagaaaa cgtagacctc gaaatcaagg ttttcactga ttatgtccaa
    10081 ttgaatccgg ctttgaaaga caaacaaatc gacgccaatt tcttccagca cattccctat
    10141 ctggaggact acaataaaaa caacaatgct gaactctctt ggacagtcaa agtccacaat
    10201 gagccaatgg gagtctattc cgaaaaagta aaggatatca acgaagtagc agacggtgcc
    10261 aaggttggta ttcccaatga tgcaaccaat ggcggtcgtg ccctgatggt actggaaaaa
    10321 gccggtttga tcaagctgac tgagggcgta ggggtcaatg ccacggacaa agatattatt
    10381 gaaaattcca agaaaattga aataaaaatg atggatgcag ccatgctgcc tagatctctg
    10441 gccgatctgg atatctgtgt gatcaacagc aattatgcga ttgaagccgg gttgaatcca
    10501 gtcaatgatt ccattttcat tgaacccaag gattcacctt ttgccaatat tctggctgtc
    10561 agaccggaag acaaggacaa agataccatt aaaaagctgg gtgctgcgct gcagtctccg
    10621 gaagtaaaga aattccttga agatacttat cagggttcct gtgtgccagc gttttaaata
    10681 acactgagcg cgcccttgta cattgacaaa aaaagtggaa gttactaact tccacttttt
    10741 ttatttgaca tgatctttaa tttcgtctga acagaataat attgctgctt taatgctgta
    10801 aatccgagga tgtttaagcc aagtctactg ccataatttt gctgggtctg ttacgatcat
    10861 gaagtatgaa tgaccatgtc atttaagatt gctggttgtt ttaatggagc ggttagggct
    10921 tattgattgc aatgaatctt gctgcttgtg gtatgtatgt tgctgcagat aaacaggatt
    10981 tgcggctgcg tatgcgataa tcttaatgta accagcaata ttaattttat taatataaag
    11041 atagaccata tgcgacataa atgcaagtat attctcttat tttcgagtaa tttttaaagg
    11101 ttgcatggta attaatgtta ttgttacaaa taaaaaagac ccgttgctca aatatttttg
    11161 agtacggatc tttttgtcgg ggttgaatca gccgatattt ttcagtaaat caaccatttc
    11221 aatggcggtc accgcagcat ctgccccttt attgcctgcc ttggtacctg ccctttcaat
    11281 cgcctgttcg attgtatccg ctgtaataat accatagatg atgggcttgc cggtattcaa
    11341 tcccacctgg gcaattcctt tggtcacttc ggccgaaaca tattcaaaat gaggcgttgc
    11401 cccccggatc acagccccca gacaaatcac cgcatcgtat tccctggccg ccattttctg
    11461 cgctgccagc ggcatttcaa aagcacccgg cacccaggca atatcaatat tttcctgatc
    11521 aaccccatgg cggatcaaag tatccataca gcctgacaat aacttgttgg tgataaattc
    11581 attgaatctg gaaactacaa tgccaaatcg ttggttttcg cctatcagtt tgccttccaa
    11641 tatttttgcc acggtacaac tcctccttta aatgaccctt atcggtgata gctctttttt
    11701 tccaattatt tattaccaga cagcagatgt cccattttat cacgtttggt atgcatgtag
    11761 cgagcatttt ccggtttgat ctccatctcg atgggaacgc gttccaccac ttccagtcca
    11821 tatccttcca accctttgat tttctttgga ttgttggtca gcaaccgcat tttccctacg
    11881 cccaggtcga ccagtatctg agccccaatg ccatagtcgc gtagatcttc ggggaatccc
    11941 aggtccagat tggcctggac ggtgtcccgt ccattgtcct gcagtttgta agcacggatt
    12001 ttgttgacca gcccgatgcc gcgtccttcc tgacgc
//
LOCUS       DAOTHZ010000064        11837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_6441_length_11837_cov_7.910542, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000064 DAOTHZ010000000
VERSION     DAOTHZ010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6441_length_11837_cov_7.910542"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="P5549_07880"
     CDS             complement(<1..592)
                     /locus_tag="P5549_07880"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRW13029.1"
                     /translation="MKQALWEQIQKTIRADQDVLLSGLDGAARIYFLHELINQRRGKL
                     LCMVASEEKAYDLANDLRLLLGENKVNLFLSRDFIFLKENSSLTEVQRVLTLQELLLH
                     PQRQGVIITTPGGMLYHIMSPQEMQERTLILVPGQDVAIEALLKQLVERGYERVDTVT
                     RPGQMAARGGLVDVFPAGDRQPCRIDFFGDTIDVIKH"
     gene            complement(694..858)
                     /locus_tag="P5549_07885"
     CDS             complement(694..858)
                     /locus_tag="P5549_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13030.1"
                     /translation="MKIYYICECCDEIFSIEENGSGTGKVEIRGICNDCALEMGLQEG
                     PALHFQTYYS"
     gene            complement(923..1477)
                     /gene="pth"
                     /locus_tag="P5549_07890"
     CDS             complement(923..1477)
                     /gene="pth"
                     /locus_tag="P5549_07890"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRW13031.1"
                     /translation="MKIIVGLGNPGKEYKDTRHNIGFMVLEEMASRHSLEKQESRFDA
                     IIGHVKIDGEKVLLVKPLTYMNLSGRSVQPIMHWYKLALEDLMVVYDDMDLPVGSLRI
                     RANGGTGGHKGMNSICERLANKEFARTRVGIGRPDRGEAYHWVLGKFSQDEKEIMQNA
                     VRHATDALETWVRQGIAAAMNAYN"
     gene            complement(1682..2635)
                     /locus_tag="P5549_07895"
     CDS             complement(1682..2635)
                     /locus_tag="P5549_07895"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRW13032.1"
                     /translation="MKASRWRMKLFTGNANPCLAQEIAAYLEIPVGDATVSHFSDGEI
                     NVAIDESVRGVDVFVVQPTCSPGNENLMELLIMIDAFRRASASRINAVIPYYGYARQD
                     RKSRARDPITAKLVSNLIVEAGAQRVVAVDLHATQLQGFFDIPVDHLPGVPTIAEYLK
                     RKGLTEDAVILSPDVGGVTRARDLAAKLAAPLAIIDKRRPAPNVSEVMNVIGDVDGKS
                     VIIIDDIIDTAGTICTAAEVMMDKGAKDVYACCSHPVFSGPAMERLEKAPIKEVIVTN
                     TIPIRKEKMLPKIKVLSVAPLVGEAILRIHEDLSVSKLFEV"
     gene            complement(2663..4030)
                     /gene="glmU"
                     /locus_tag="P5549_07900"
     CDS             complement(2663..4030)
                     /gene="glmU"
                     /locus_tag="P5549_07900"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRW13033.1"
                     /translation="MVCSAVILAAGKGVRMRSHIPKVVHPVAGIPMVKHVAQAITAAG
                     INKITLVIGQQHELIKDLFAGQNFQYAVQAEQLGTGHALLQARSEVESDDMVLVVAGD
                     TPLLQAETLQQLIAHHRNCQATATVLSTTLDDPFGYGRIVRDQDGRLERIVEEKDANP
                     EEKKIDEINSGMYCLQVKEAFAALQKLGSSNAQGEYYLTDILSILKNKGHRVEVFLTQ
                     SRDDIYGINNREQLAWAEKIMRRRKNHQLMMDGVTIIDPDTTFIDSHVSIGMDTTIYP
                     FTIIEGETTIGESCHIGPSSHLNDARIGNQVVIENSRIKEAEIGDECTIGPFAYLRPG
                     AVLRQGVKVGDFVEIKKSIIGPGSKIPHLSYVGDAKLGSDVNVGAGTITCNYDGKNKW
                     PTILEDGVFIGSNTNLVAPVRIGRNAVTGAGSTITRDVPADTLAVERANQKHIEGYTK
                     KKDRS"
     gene            complement(4245..5027)
                     /locus_tag="P5549_07905"
     CDS             complement(4245..5027)
                     /locus_tag="P5549_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRW13034.1"
                     /translation="MQYDAIILAGGESTNDLKKIAPYDNEALIIIGNYPMIYFVYQTL
                     RSSSQINNIVVSGPVEALKAILPRDERLFFVNGGQNAIESFAHAVKALNDKGISSKVL
                     IMPTDIPFITVEAIEDFIKRCETSDDDFYYPVTRKEVNEKKFPGVQRTYVRLQEGVVT
                     GGNLFLIRSSVIEKSLEMGLKLVERRKNPLAMARLFGISLALRYLFGRLSIAMVEKRF
                     YKMLGIKGKCIISPYAEIGVDVDKPSDLKLAQDKLGSICFAK"
     gene            complement(5041..5736)
                     /locus_tag="P5549_07910"
     CDS             complement(5041..5736)
                     /locus_tag="P5549_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRW13035.1"
                     /translation="MAEKRLLPVNLDSYKPLRELVLEAIREAIKNGTLQPRERLMEIQ
                     LADELGVSRTPVREALRKLELEGFIVMVPRKGAYVSDLSMKDVADVFEIRAALEGLAG
                     ALAAERITEEELEAMERMLVEKGEAISLNDIDKLVAADTKFHEAMYMASRNERLSNII
                     SNLREQIQRFRLTSLSVPGRRQDSLKEHREILEAIQARDIQLAKQLAQEHIENAENVL
                     IDSLKKGNQPASE"
     gene            complement(5736..6626)
                     /gene="ispE"
                     /locus_tag="P5549_07915"
     CDS             complement(5736..6626)
                     /gene="ispE"
                     /locus_tag="P5549_07915"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRW13036.1"
                     /translation="MAMKSSLSDCRCSLEAPAKVNLTLDVLGKRPDGYHELATVMHQI
                     DLKDKITLEKGGTKIRLDSNSSLIPNDEKNLAYQAARLMCENFSLREGLQIYLEKNIP
                     VGAGLAGGSTDAAAVIIGINELFALKQELAVLLALGARIGSDVPFCIMGGTALARGRG
                     EILSPLPEGLRLHMVLVKPDFQISTRAVYQAFRREKVTRTPDTAAFLAAWQDCDIISI
                     AREMNNVLESVSLAEHPEIAAIKKQLVSAGAVNALMSGSGPSVFGLFINPETAVEAFN
                     ILHQQYNESFIVSSYRRGWK"
     gene            complement(6764..7327)
                     /gene="spoIIR"
                     /locus_tag="P5549_07920"
     CDS             complement(6764..7327)
                     /gene="spoIIR"
                     /locus_tag="P5549_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HRW13037.1"
                     /translation="MFRKIMIFIVLLVFVCSGIFVASAQESRLDHSVLRLHVVANSDA
                     PADQALKMAVKDEIVRYLQAEFSHINDVDEAQAMAQKNRGQIESIAQQKIKTSGYDYP
                     VEVTIGEFAFPLKSYGNMVFPAGEYQAVRVVIGKGAGKNWWCVLFPPLCLVSSADRGM
                     SLQSVSEAQVTFKCLELLPQGVRIKQL"
     gene            7498..8262
                     /locus_tag="P5549_07925"
     CDS             7498..8262
                     /locus_tag="P5549_07925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRW13038.1"
                     /translation="MQHIKIEGSLLAYQSSRMDNAQGSILFVHGSGGDSHIWEEQLTH
                     LPPDFCGIAIDLPGHGSSAGDLLATVDAGARLISELPALLNLPRPLWLAGHSMGSAMA
                     ITAALDYPKQVDGLILIGSGARLRVLPQALAALKAGIMKLEFLKIGFSTATPAAIWEK
                     EVEALQKAGPKIIHNDFLACDQYDRSADLAQITVPTLIIVGQDDLLTPLKYSEFLHEH
                     ISSSKLVVIPEAGHYVMLEKPAETSQAITDFLLKTV"
     gene            complement(8379..11582)
                     /locus_tag="P5549_07930"
     CDS             complement(8379..11582)
                     /locus_tag="P5549_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRW13039.1"
                     /translation="MNERMHDGDYSLDNLKERAKELNCLYQVDEILNNRHLSLAEIFS
                     ALVKVIPSGWQFPEFCKVLIVYENRRYYTEGFSASTISESCDIKVNGKVIGRIEVVYV
                     RKVNKSEEGYFLDKERKLIRTIADRIGQTIFQRYMGQLLRQWDNSRQEPLAGNAKREW
                     MVIVDLIGRSNQNALLHICRKMANHLYLSGVKEAEEVLWDFGSGFRGDYAGEVNYPSE
                     KFPLGNLQQISQKIFRIAEQHLSDDEISIRLKQWIEEEKAFPLIKAVDRIDVSVREIV
                     KLLNRYKNSYGDTNRPDFPMERWLVVALIQRFLSEKLDFINIARQHLEIIDFYAIVNQ
                     LIFPEGSHGKIGGKGTGLFLAEQVLAKAQEENPLLKSVKVPRTWYISTDEIKEIIHYN
                     NLEELNVQKYKDLYEIRLDYPNIIQILKNAEFPQGIIKSLAMALDDFGDVPLIVRSSS
                     LLEDQMGAAFSGKYKSLFLANQGSKQERMEALVNAIMEVYASIYSPDSIQYRSERGLL
                     DFNEEMGILIQEVVGRRVGVYYLPVYAGVAFSHNEFRWSPRIKREDGLLRLVMGLGTR
                     AVDRLSDDFPVLISPGQPGLRVNTVPQEIQHYAPKKVDLINLEKNTFDTVEIAALVKQ
                     FGHDVPQLQQYFSIYQQDYVKKPLAFEIDCENDEVLLTFDGLIADTTFVKQVKLILEV
                     LEEKLATPVDIEFASDGEHFYLLQCRPQSSAPSLAPAPIPKEIPAKDIIFSARRYISN
                     GTVNDISHIVYVDPNEYNRLTELEDLVNVGKAVGMLNLLLPRHRFVLIGPGRWGSRGD
                     IKMGVQVSYADINNTAALIEVARKKSNYIPELSFGTHFFQDLVEAQIRYLPLYPDDPG
                     MVFNERFLRRTSSILAKILPEYARLEEVIRVIDVPASTNGRILKIVMNADLEEALAYL
                     AEPAGMIDAAARDGLGETWTARAEGDYVDDRFWRWRYYMAERLAERLDFDAFGVKGLY
                     LFGSTNNGTAGPGSDIDLLVHFQGNPVQREQLKLWLQGWSISLAEMNYLKTGYATDEL
                     LDIHIITDEDLVNKSAFAIKIGAVTDPAHPLKIKQDY"
     gene            complement(11628..>11837)
                     /locus_tag="P5549_07935"
     CDS             complement(11628..>11837)
                     /locus_tag="P5549_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HRW13040.1"
                     /translation="TCSYFEQVQCNNNFYWEKDEVLLRLDQKMTAAFKATKETADKNK
                     LYMRDAAYVIAINRVAQAVKMRGWV"
BASE COUNT         2904 a         3179 c         2559 g         3195 t
ORIGIN      
        1 aatgcttgat tacgtcgatg gtatcaccaa aaaaatcgat ccggcagggc tgcctgtctc
       61 cagccggaaa cacatcgacc aatcctcccc gggcagccat ttgcccaggc cgggtcaccg
      121 tatcaactct ttcataacct ctttcgacca actgtttcaa taatgcttcg atagcaacat
      181 cctgacccgg taccagtatc agagtgcgct cttgcatttc ctggggcgac ataatatgat
      241 acagcattcc tcccggcgtg gtgataataa caccctgccg ctgcggatgg agcaatagtt
      301 cctgcaaggt cagcacccgc tggacttcag tcagcgatga gttctccttc aagaagataa
      361 aatcacggga caagaaaaga tttaccttgt tttctcccag caacagtctt agatcgttag
      421 ccaggtcata cgccttttct tctgacgcca ccatgcacaa tagcttgcct cgccgctggt
      481 taatcagttc atgcagaaaa tagatccgcg ccgccccgtc aagaccgctc aaaagaacgt
      541 cctggtcagc acggatggtt ttttggattt gttcccataa cgcttgtttc aactctcctg
      601 gtcctcccct gaaacacttt ctgccaaacc cacaaagtgg acaccgtaaa aatggcctcc
      661 acttcttggc agtattcatt taatcttttg gacttaactg taatatgtct gaaaatgaag
      721 cgctggtcct tcctgcaaac ccatttccag cgcgcaatca ttgcaaattc ccctgatctc
      781 caccttgcct gtaccgcttc cattctcttc gatactgaaa atctcatcac agcattcaca
      841 aatatagtat attttcattt gcttgctcct tcgcccgttt ttattatctt cccaggcgaa
      901 agggtaattt atacattttt gtttaattgt atgcattcat ggccgctgct attccctgcc
      961 ttacccatgt ttccaaggcg tctgtcgcat gccggacggc attttgcatg atttccttct
     1021 cgtcctggct gaattttccc aaaacccaat ggtaggcttc tcctctgtcg ggtcttccaa
     1081 ttccaacccg ggtacgggca aactccttgt tggccaggcg ctcacaaatc gagttcatcc
     1141 ctttgtgccc tccggtccct ccattggcac gaattcgcag actgccaacc ggcagatcca
     1201 tgtcatcata aacgaccata aggtcttcca gggcaagttt gtaccagtgc attatgggtt
     1261 gtactgaccg tccgctcaga ttcatataag tgagcggttt gaccagcaga accttttcgc
     1321 cgtctatttt tacatgacct atgatggcgt caaatctgct ttcctgtttc tccagcgaat
     1381 gacgcgaggc catttcttcc aacaccataa agccgatatt atggcgggta tccttgtatt
     1441 ctttgccggg attgcccaag cctacaatta ttttcatgct gtttacctct ggttttgtct
     1501 gccaaaatta ttatcagtat aacctgaacg acactgcaaa aaaagtctgc aaacgtatct
     1561 gaacaagttg taaatgcgac aaaataatag aagactgccc aatcaacata cctgcgtacg
     1621 cttcttgcac agtctcataa tcctgatatg gtgccattaa aagcgaatca ttgcttcatt
     1681 tttatacttc aaacagcttg ctgacagaaa gatcctcatg aatcctcaag atcgcctcac
     1741 ccaccagcgg tgcaaccgaa agaaccttta ttttgggcag catcttttct tttcttatgg
     1801 ggatggtatt ggttacgatg acctccttga tcggggcctt ctccaatctt tccatggccg
     1861 gaccggaaaa taccggatgg ctgcagcagg cataaacatc cttggcgcct ttatccatca
     1921 tcacctctgc ggcagtacag atggttccgg ctgtgtcaat aatatcatcg atgatgatga
     1981 ccgattttcc atctacatcg ccgattacat tcatcacttc ggaaacattg ggtgcaggac
     2041 ggcgtttatc gataatcgcc agcggcgctg ccaatttggc cgccaggtca cgggcccggg
     2101 ttacacctcc cacatcaggg gagagaatta cggcatcctc cgttagacct ttacgtttga
     2161 gatactctgc gatggtcggt acacccggca agtgatccac cggtatatca aagaatccct
     2221 ggagctgtgt ggcatgtaaa tcgactgcca cgactcgctg ggctccagcc tcaacgatca
     2281 gattggacac cagcttggca gtaatgggat cacgggcccg gctcttgcga tcctgacgcg
     2341 cataaccata atagggaatg actgcattga tgcgcgaagc cgaagcacgt ctgaaagcat
     2401 cgatcatgat caataattcc atgagatttt cattgccggg tgagcaggtg ggctggacaa
     2461 cgaacacatc tacccctctt acactttcat caattgctac attaatttct ccatctgaaa
     2521 aatggctgac ggttgcatct cctaccggga tttccagata agctgcaatc tcttgcgcca
     2581 gacagggatt ggcgttgccg gtaaatagtt tcatcctcca cctggatgct ttcatactcc
     2641 ttatacctcc caaattattc tctcaggacc tgtctttctt tttcgtatat ccttcaatat
     2701 gcttttgatt ggctcgttcg accgccagag tatctgccgg aacatccctg gttatggttg
     2761 agccggcacc ggttacggca ttcctcccga ttcgtaccgg cgcaaccaga tttgtattgc
     2821 tgccgataaa aaccccatcc tccagaatgg tcggccattt gttttttccg tcgtagttgc
     2881 aggttatggt tccggcaccg acattgacat cactgcctag ttttgcatcg cccacgtaac
     2941 ttaaatgggg tatcttgctg ccgggtccga taatggattt tttaatctcc acaaaatccc
     3001 cgacttttac accctgccgc agaactgctc ccggacggag ataagcaaaa ggaccgatag
     3061 tacattcatc cccgatctcg gcttctttaa ttcttgaatt ttcaatgaca acttgattgc
     3121 caatcctggc atcattaagg tgtgaactgg gtccgatgtg acaggattct ccgatggtcg
     3181 tctcgccttc aattatggta aacggataaa tggtggtatc catgccaatg ctgacatggc
     3241 tatcaatgaa ggtcgtatcc ggatcgataa tggttacccc gtccatcatc aactgatggt
     3301 ttttgcgccg gcgcatgatt ttttctgccc aggccagctg ttcacgattg ttaatcccat
     3361 aaatatcgtc ccgggactgg gtcagaaaca cctcaaccct gtgacccttg tttttcaaga
     3421 tggacaggat gtcggtcaga taatactcgc cttgcgcatt actggatccc agtttttgca
     3481 aggccgcaaa ggcctcttta acttgcaggc aatacatgcc ggagttgatc tcatcgatct
     3541 tcttctcttc aggattggca tctttttctt ccacgatgcg ctccaaccgg ccgtcttggt
     3601 cacgtacgat gcggccatat ccaaaaggat catccagtgt cgttgacaaa accgtcgcag
     3661 tggcctgaca attccgatga tgggcgatga gctgctgcag cgtttcggct tgcaaaagcg
     3721 gggtatcccc ggcaacgacc aaaaccatat catcactttc gacttcgctg cgggcttgca
     3781 gaagagcatg cccggtcccc agctgttccg cttggacagc atattgaaaa ttctgtccgg
     3841 caaacaaatc ttttatcaat tcgtgctgct gcccgatcac caaagtaatc ttgttgattc
     3901 ccgccgcagt gatcgcctgc gcaacatgtt tgaccatggg aattcccgca acgggatgga
     3961 cgactttggg aatatgcgag cgcatgcgca cgcccttgcc ggcagccaga atgactgctg
     4021 aacaaaccat acctattcct ccctgttggc tcaagtcaaa aataggaacc aaacgggctt
     4081 cgcactctgc ttcaccaaat gtttgatacc tattcattat ttgctcttaa cttaatacta
     4141 ctgtattata cctgcatttg taaaaacgaa aaagtatttt gctgtcaggg gttgccaacc
     4201 gctaactgca tacaaggata aaccagccta atcaggctgg ttggtcactt ggcaaaacat
     4261 atggacccaa gcttgtcctg ggccagtttc aagtcgctgg gcttgtcgac atcaacccca
     4321 atctccgcat agggggagat gatacatttg ccttttatgc ccagcatttt ataaaaccgc
     4381 ttttcaacca tggcaatgct gagccggcca aaaagatacc tcaatgccaa agaaatgcca
     4441 aaaagcctgg ccatagccag gggattttta cgtctttcca ccagctttaa gcccatttcc
     4501 agacttttct caatcaccga gctgcggatc aggaataaat tcccacccgt cacgacgcct
     4561 tcctgtaaac gcacataggt cctctgtact ccgggaaatt tcttttcgtt gacttctttg
     4621 cgtgtcactg gataataaaa atcatcatcg ctggtttcac atcgtttgat aaaatcttca
     4681 atcgcttcca cggtgataaa gggaatatcg gtaggcataa tcaacacttt ggaactgatc
     4741 cctttgtcat tgagcgcttt gacggcatgg gcgaagcttt caatagcatt ttgacctcca
     4801 ttgacaaaaa acagcctctc atcccgaggt aaaatcgctt tcagggcttc gaccggtccg
     4861 ctgaccacaa tattgttgat ctgggatgaa gaacgcaagg tttgatatac aaaataaatc
     4921 atcggataat ttccaataat aattagggct tcattatcat agggagcgat ttttttcaaa
     4981 tcattggtgc tctccccgcc agccaaaatg atggcatcgt attgcatgtt gttccctcca
     5041 ttattcgctt gcaggttgat tccccttttt cagcgaatcg atgagcacat tttcggcgtt
     5101 ttcaatgtgc tcctgggcca gttgtttggc cagttggata tctctggcct gaatggcctc
     5161 caatatttcc cggtgctcct taagtgaatc ctggcggcgt ccgggtacag aaagtgaagt
     5221 taatctaaaa cgttggattt gttctcgcag attgctgatt atattactca gcctttcatt
     5281 gcggctggcc atgtacatgg cttcatggaa ttttgtgtca gctgccacca gtttgtcaat
     5341 atcattcaga ctgattgctt cgcctttctc taccagcatg cgttccatgg cttccagttc
     5401 ttcttcggtg atcctctctg ccgccaaagc cccggccaat ccttccagcg ccgccctgat
     5461 ttcaaataca tccgctacat ctttcatgga aagatctgaa acatacgctc ctttgcgagg
     5521 caccatcacg ataaagcctt ccagttccag cttgcgcagg gcttcccgca ccggggtccg
     5581 ggagacccct aattcatctg ccagttggat ctccattagt ctttcccgcg gttgcagagt
     5641 tccatttttg atcgcttcgc ggatggcctc caataccaat tcccgcaggg gtttgtacga
     5701 atccagattg accggcaaaa gacgtttttc tgccattact tccaccctct cctatatgaa
     5761 gatacgataa atgattcatt atattgctgg tgcaatatat taaaagcttc taccgctgtt
     5821 tccggattga taaacaaacc gaaaacactg ggcccgctgc ccgacatcag ggcattgaca
     5881 gcgcctgccg acactaactg ctttttaatg gcggcgattt caggatgctc ggccaggctc
     5941 actgattcca atacattgtt catttcccgg gctatactaa tgatatcaca atcctgccag
     6001 gctgcaagaa aagccgctgt atcaggtgtt cttgttactt tttcccgacg aaaggcctga
     6061 tacactgccc gtgtggaaat ctgaaagtca ggttttacca gcaccatatg caacctcaat
     6121 ccttccggca aaggactgag aatttccccc cgcccccggg ccagcgctgt acctcccatg
     6181 atacagaagg gcacatcgga accgattcga gcccccagcg caagcaaaac agccaactcc
     6241 tgctttaagg caaacaattc atttattccg ataatgactg ctgctgcatc tgtgcttcct
     6301 cccgccaggc ccgcacctac aggtatattt ttctccaagt atatctgtag tccttcccgc
     6361 agggaaaaat tctcgcacat gagccgggcg gcctgatagg ccagattttt ttcgtcgttc
     6421 ggaatcaaac tgctgttgct gtccaagcgg attttggtgc cgcctttttc cagtgtgatc
     6481 ttgtccttca ggtcaatctg atgcataacg gtggccagtt catgatagcc atcggggcgt
     6541 ttgcccaaaa catccagggt caggttcacc ttggcaggag cctccagcga acaacgacaa
     6601 tcagataagg agcttttcat tgccatgtac cgtctctcca attgtttttc ccattatatc
     6661 gctttgccgt ttattcgtta atatttttca ttttcgttat tcatgcttcc cctgtcaaac
     6721 caaacttcct tgtcgcaggc tgtcccttat cttggcatgc gcgctaaagc tgcttgatac
     6781 gcaccccttg gggtagcagt tccaggcatt tgaaggttac ctgggcttct gaaacacttt
     6841 gcaggctcat gccccggtcc gcagaggaaa ccaaacacag cggaggaaac agcacgcacc
     6901 accagttttt gccggcccct ttgccaataa ccactcggac tgcctgatat tctccggctg
     6961 gaaagaccat gttaccgtag gatttaagtg gaaaagcaaa ctcgccaatg gttacttcaa
     7021 ccggataatc atacccgctg gttttgattt tttgttgggc gatgctttct atttggccac
     7081 gatttttttg ggccattgcc tgtgcttcat caacatcatt aatatgggaa aattctgctt
     7141 gcagataacg tacgatttca tccttgacgg ccattttcaa agcctgatcg gcgggtgcgt
     7201 cactattggc tacgacatgc aaacgcagca cggaatgatc taatcggctt tcctgcgccg
     7261 aggcaacaaa aatacctgaa caaacaaaaa ccagcaagac aatgaatatc attatttttc
     7321 tgaacatcgg tccatccctc ctcatgctca attattgcca tgcatctcac cgcttaaacg
     7381 cattcctggc gcaagtgcct gaaaccgttc gcgggagtga taccgacaac cgctgttgct
     7441 cccggtcaaa atcctttata ctgtttacaa aaagcaatga aaaggatgat ggcagacatg
     7501 cagcatataa agattgaggg aagtttgctg gcttaccaat ccagcagaat ggacaacgcc
     7561 cagggaagta tcctgtttgt acatggcagc gggggtgaca gtcacatctg ggaagaacag
     7621 ttaacccatc tcccccctga tttctgtgga atcgccatcg acttgcccgg tcacggctct
     7681 tctgccggag atttgctggc aacggttgat gccggcgccc ggctgatcag cgaattgcca
     7741 gcactgctca acctgcctcg tcccctctgg ctggcaggac actccatggg ttcagccatg
     7801 gccattacgg ctgcgctgga ttatcccaaa caagtcgacg gtctcatcct gattggctca
     7861 ggagcacgtt tgcgggtcct gccccaggcg ttggcagcgc tgaaggccgg cataatgaag
     7921 ctggaatttt taaaaatcgg gttttctacg gcaaccccgg cagccatttg ggaaaaagaa
     7981 gtggaagccc tgcaaaaagc gggtccaaaa ataatccata atgatttttt agcctgtgac
     8041 caatacgacc gcagcgccga cctcgctcag atcacagttc ccacgctgat catcgtgggc
     8101 caggatgacc tgttgacccc tctgaaatac tcggaattcc tgcatgaaca tatttcctcc
     8161 tcaaaactgg tcgtgatccc ggaggcaggc cattatgtaa tgctggaaaa gccggcagag
     8221 accagtcagg caattactga cttcttactg aagacggtct gacacaaccc ctaaagtcat
     8281 ttttcatgtc atcctgagcg ttagcgaagg atctctaagc agcatctcct gatgagatcc
     8341 ttcacttcat tcaggatgac aacaatgtta ttttccgact aatagtcctg ctttattttt
     8401 aaaggatggg ccggatctgt gaccgcgccg atcttgatgg cgaaggccga tttattgacc
     8461 agatcttcat cggtaatgat atgaatatcc aacagctcgt cggtggcata gccggttttc
     8521 agataattca tctccgccag actgatgctc caaccctgca gccagagctt caattgctct
     8581 cgttgcacgg gattgccctg aaaatgaacc agcagatcga tatcactgcc cggaccggca
     8641 gtgccgttat tggtactgcc aaacaaatat agccctttca ctccgaatgc gtcaaaatca
     8701 agtctttccg ccagacgttc tgccatataa tagcgccagc gccaaaagcg atcgtcaaca
     8761 tagtctcctt ctgctctggc cgtccatgtt tcacccaggc cgtcccgggc cgccgcatcg
     8821 atcatgccgg ccggctcagc cagataggca agcgcctcct ccaggtccgc gttcatgaca
     8881 atttttagga ttcttccatt ggtgctggcg gggacatcaa tcacccggat cacttcttcc
     8941 agcctggcgt attcgggcag aatctttgcc agaatgctgg acgtacgccg caaaaaccgt
     9001 tcattgaaaa ccattcccgg atcatccgga tagagcggca aataccgaat ctgggcttcg
     9061 accagatcct gaaagaaatg tgtcccaaag gagagctcgg gaatataatt ggattttttg
     9121 cgggcgactt caatcagcgc agcggtattg ttgatatcag cataactcac ctgaacgccc
     9181 attttaatgt ctccgcggct tccccagcgg cccggtccga tcaggacaaa ccggtggcgg
     9241 ggcaagagca ggttcaacat gccgacagcc ttgcctacat tgaccaagtc ctccagctcc
     9301 gtgagccggt tatattcgtt gggatcaaca tacacgatat gagatatatc attgaccgtg
     9361 ccgttggata tataacgccg ggcagagaaa ataatatcct tggccggaat ctctttggga
     9421 atgggcgcag gagccagcga gggggccgaa ctttgcggcc ggcactgcag cagataaaaa
     9481 tgttcgccgt cgctggcaaa ttcaatgtca accggcgtag ccagcttttc ctccaagact
     9541 tccaggatca gcttgacctg tttgacaaaa gttgtatcgg caatcaagcc atcaaaagtc
     9601 agcaaaactt catcattctc acaatcaatc tcaaaagcca gcggcttttt tacatagtct
     9661 tgctggtaaa tggaaaaata ctgctgcaat tgtgggacat catgcccaaa ctgtttgacc
     9721 agcgcagcaa tttcaacggt atcaaacgta tttttttcca gattgatcaa atccactttc
     9781 ttgggagcat aatgttggat ctcctggggg acggtgttga ctctaagtcc aggctgtccg
     9841 ggagaaatta aaaccggaaa atcatcactc agccggtcca ctgcccgggt ccccagtccc
     9901 atgaccaaac gcagcaagcc atcttctctt ttaatgcggg gcgaccagcg aaattcgttg
     9961 tgactgaagg cgactccggc ataaaccggc aaataataaa cgcccacccg tcggccgacc
    10021 acttcctgaa tcagaatacc catctcttca ttgaaatcca gcaaccctct ttccgagcga
    10081 tactgaatcg agtcgggact gtagatggag gcatagacct ccatgatcgc attgacaagc
    10141 gcctccatac gctcctgctt gctgccctga ttggctaaaa aaaggctttt atatttgccg
    10201 gaaaaagctg cccccatctg atcctccagt aaactggagc tgcgtacgat caaggggacg
    10261 tcaccaaaat cgtccagcgc catggccaaa ctcttaatga taccctgggg aaattcagcg
    10321 ttcttcagga tttgaataat gttggggtag tcgagcctga tttcgtagag atctttatac
    10381 ttttgcacat tcagttcttc tagattgttg tagtgtatga tctccttgat ctcatcggtg
    10441 ctgatatacc aggtgcgggg gaccttcacg gactttaaca gggggttttc ctcctgggcc
    10501 ttggccagaa cctgctctgc cagaaatagc ccggtgccct tgcctccaat tttgccatga
    10561 cttccttcgg ggaaaatcag ctggttgaca atggcataga aatcaatgat ctccagatgc
    10621 tgccgggcga tattgataaa atccagcttt tcagataaaa atctttgaat aagcgcaaca
    10681 accaaccatc tttccatagg aaaatccggc cggttggtgt cgccataact attcttgtaa
    10741 cgattgagca acttgacaat ttcccgcacc gaaacatcga tacgatcgac tgccttgatt
    10801 aacgggaaag ctttttcttc ttcaatccat tgtttcaagc gaatgctaat ctcatcatcg
    10861 ctcaaatgct gctcggcaat gcgaaaaatc ttctggctga tttgctgcag attgcccaag
    10921 ggaaatttct cactggggta gttgacttct ccggcataat cgcctcgaaa gccggaacca
    10981 aaatcccaca ggacctcttc tgcttccttg acaccgctca gatatagatg attggccatt
    11041 ttgcgacaaa tatgcaagag cgcattttga ttgcttcggc caattaaatc gacgatcacc
    11101 atccattccc gcttggcatt gcccgccaat ggttcctggc gggaattgtc ccattggcgc
    11161 agcaattgtc ccatataacg ctggaagatg gtctggccga tacggtcagc aatcgttctg
    11221 atcaatttcc gttccttgtc caggaaataa ccttcctcgc tcttgtttac cttgcggaca
    11281 taaaccactt cgatccggcc aatcacctta ccgtttactt tgatgtcaca gctttctgaa
    11341 atcgtagagg cggaaaaacc ttcagtataa tagcggcggt tttcatacac gattaaaacc
    11401 ttgcaaaact caggaaattg ccagcccgag ggaatgacct tgaccaaggc ggaaaaaatt
    11461 tccgccaggg acaaatgacg gttgtttagg atttcatcca cctgataaag acaattaagc
    11521 tccttggccc tctccttcag gttgtcgaga gaataatcac cgtcatgcat ccgctcgttc
    11581 atatcatccc cgccttgtag tttttctcta caatatattt tacccgatta tacccagcct
    11641 ctcatcttta ccgcctgcgc aacccggttg atggcgatga cataggctgc atctctcatg
    11701 taaagcttgt ttttatccgc agtttccttc gtggctttga aggctgcggt catcttttgg
    11761 tcaagtctta acaaaacttc gtctttttcc cagtaaaaat tattattgca ctgcacttgc
    11821 tcaaaatagc tgcaggt
//
LOCUS       DAOTHZ010000065        11349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_6797_length_11349_cov_5.983354, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000065 DAOTHZ010000000
VERSION     DAOTHZ010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6797_length_11349_cov_5.983354"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="P5549_07940"
     CDS             <1..528
                     /locus_tag="P5549_07940"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042547.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRW13041.1"
                     /translation="PDNVLDRIVAAGMKVAGIGKIHDIFAGKSISTTEHIENNRDGLA
                     KTVQAIAGEAFSGLLFVNLVDFDQQYGHRNDAHGYAGALEEFDRHLSEVMAVLQAGDI
                     LFITADHGCDPTTSSTDHSREQVPLLVYGQPVKKDVLLPVRTSFADLGQTIAEYLGIE
                     ADSLPGESFYAFIRR"
     gene            532..1836
                     /locus_tag="P5549_07945"
     CDS             532..1836
                     /locus_tag="P5549_07945"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HRW13042.1"
                     /translation="MNVIEMITKKRDGGEMNQAEIEFMVKAISRGSIPDYQISAWAMA
                     VYFQGMSDEETTALALAMAYSGEVMDLSALEGIKVDKHSTGGVGDKTTLVVIPLAAAA
                     GVPAAKMSGRGLGHTGGTIDKFEAIPGFQVEMNREQFLQQVARIKAAVISQSGNLVPA
                     DKKLYAIRDVTATIESIPLIASSIMSKKIAAGAQGIVLDVKVGQGAFIKDRVQALQLA
                     ETMVRIGRGAGRRVVALLTDMSQPLGRFIGNALEVREAIDLLQGQGPDDLRQLCIELA
                     AHMLVLGGQADELETAREKLRVLLASGAALAKFDELVVAQGGQLHYNQPDCGLPQASI
                     KEKVAADRSGFVTGLDALKVGHSAMLLGAGRERLDDVIDPAAGIELLKKCGDYVQAGE
                     PLAILHANCQERLAQVRPLLRAAFQLGVTAEGIPPLIYQTIE"
     gene            1863..2969
                     /locus_tag="P5549_07950"
     CDS             1863..2969
                     /locus_tag="P5549_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRW13043.1"
                     /translation="MTWHSFLPGHIYFGNKVIQQNPEMFRGLGRQALIVTGQGGSARR
                     IGALDDITQALQQQDIQWEIFDQVEANPSIATVRDGGQRARQSGADFIIAIGGGSPMD
                     AAKAIAVLALHDISDDQLLALQFGEALPLVAVPTTAGTGSEVTAASIITFPEREAKLG
                     IRDNKLLPRVAILDPGYTVSLPWQITADTAVDAYSHAMESYLSVIATPISDMHARTAL
                     DILGPQLKRLTASREIGMADREALLLGSLLAGMAISETRVTIPHLLGYTFTIYKDIPH
                     GRANGLLMPAFMTFNLEKSQDPKVKQVLEYSHFKDLQELTEVLAQLCGPPPVCSEAER
                     QHFVDRTTGNPSLANNLVAPDREDLAAMLARALQ"
     gene            3066..4211
                     /locus_tag="P5549_07955"
     CDS             3066..4211
                     /locus_tag="P5549_07955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRW13044.1"
                     /translation="MKKKCAVACILMVVFFLGLAPPVLAGAELETVKAEAYIMTEADS
                     GKVLLAKNENKRLPPASMTKLMTLLLAAEALEEGRVTLKEKVSTSDHASQMGGSQIYL
                     APGEEMTFEDMLIAVAVGSANDASVAVAEKLEGTHLAFVDKMNAKAKELGMKNTQFKN
                     SNGLPATDHFTTAADMAILARAALRQPRVMEYCSIKEYPLRGGKFILYNTNKLLWWYK
                     GTDGFKTGRTEEAKNCLTSTVKRDGLRLIAVVMASPETRGHFRDSMEMYNYGFARYAY
                     KTFVPRNKMCGTVKVGKGRQEQVEVLAQEDAGALFVKGEKAKITSKQMLLKYVDAPVS
                     KGEKLGEVQIYKNGTKIKTVALIAAQNVERGGILRQIKKMFVETYLL"
     gene            4289..4621
                     /gene="spoIIAA"
                     /locus_tag="P5549_07960"
     CDS             4289..4621
                     /gene="spoIIAA"
                     /locus_tag="P5549_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HRW13045.1"
                     /translation="MKIECKQVRSTLVVKLSGEFDMMIADKLRREIDSRLESKQITNL
                     IINLEKVTFIDSSGLGVIIGRYKKISSQKGHMYIVGASASVEKILTFSGINKLIPIYK
                     NEQDIINI"
     gene            4656..5102
                     /gene="spoIIAB"
                     /locus_tag="P5549_07965"
     CDS             4656..5102
                     /gene="spoIIAB"
                     /locus_tag="P5549_07965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HRW13046.1"
                     /translation="MNIRNYMRIEFPSLPENVSFARASVGAFAAQLDCTLEEIEEVKL
                     VVSEAVSNCIIHAYDNRSNEKIMVMASIGLERELEILVEDTGKGIADISQAMEPTYSS
                     DETRMGMGFTFMKSFMDNVEVRSIVGEGTSVTLRRTFLYHDKEALA"
     gene            5119..5847
                     /locus_tag="P5549_07970"
     CDS             5119..5847
                     /locus_tag="P5549_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HRW13047.1"
                     /translation="MPLENKYDDNEKMVERAQNGDAQARAQIYENNLGLVYMVLERFK
                     NSSYEYEDLFQIGSIGLLKAIEKFDFAYQVRFSTYAVPMIIGEIKKFLRDDGIVKVQR
                     GLKETYGKVRWAQDKLRGDLGREPGIAEIASLLEIDKEEIVLAMEACQSPAYMQDVMP
                     GEKDQLSLIDKLANEDGNVVLLEQMALREALAKLEEREREVLLRRYFKDETQMVIAED
                     LGVSQVQVSRIEKAALRKLKNLLA"
     gene            5944..6138
                     /locus_tag="P5549_07975"
     CDS             5944..6138
                     /locus_tag="P5549_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HRW13048.1"
                     /translation="MQIKVAEIVGESKKDWQDAVKNAVKEASREMGNITGVEVYNWTA
                     DCQDDQIIEYKANIKIAYTD"
     gene            6350..6814
                     /gene="spoVAC"
                     /locus_tag="P5549_07980"
     CDS             6350..6814
                     /gene="spoVAC"
                     /locus_tag="P5549_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HRW13049.1"
                     /translation="MQEAKSEELKSLEKKEYQNLVNQVKPKPPLLKNFLWAFGVGGFI
                     CVIAQMLLNYLLFRGMSETDAGSSVAVVMVFLGALLTGIGVYDKIGEKAGAGSIVPIT
                     GFANVIVASAMEFKREGYIFGVGARIFSIAGPTLLFGFTASVLIGLISYWLK"
     gene            6834..7847
                     /gene="spoVAD"
                     /locus_tag="P5549_07985"
     CDS             6834..7847
                     /gene="spoVAD"
                     /locus_tag="P5549_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HRW13050.1"
                     /translation="MTAARKQGQQSVIFAHPPVLSAWASIVGPKEGQGPWARDFDKIL
                     DDYLDGQSSWEKAENKLLQTTVKTAVAKAGLDITEAELLLAGDLLNQIISANYAAREL
                     NVPFLGLYGACSTMAESLLLAAMLVDGGFFERVVAATCSHHYTAERQYRAPVEQGVQP
                     SPSAQWTATASGAVLVQPGGNGPRITCATIGKVVDMGITDAANMGAAMAPAAGSTIQA
                     HFADTGRPSDYYDLVVTGDLGTVGMKLLKRIFVQHGIVPQPVYSDCGVLIYDGLPNVK
                     AGASGCGCSAAMLCGPLLQKLSRGEINRLLFVATGALMSPISTFQGESIPGIAHALAI
                     ENT"
     gene            7890..8246
                     /gene="spoVAE"
                     /locus_tag="P5549_07990"
     CDS             7890..8246
                     /gene="spoVAE"
                     /locus_tag="P5549_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HRW13051.1"
                     /translation="MSYVTAFLLGGCLCVIGQLVMDLTKPGITPGHVLVGFITGGAIL
                     SAVGLYQPLVDWGGAGATIPLSGFGHSLAQGAIDGAREKGLLGVFSGGVAATAAGITA
                     AIVFGYLAAVIFNPKD"
     gene            8262..9326
                     /locus_tag="P5549_07995"
     CDS             8262..9326
                     /locus_tag="P5549_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRW13052.1"
                     /translation="MQRVGIPQGLFYHYYYPLWKTFFTDLGAEVVSSGPTNRTTVEQG
                     IAAAVDESCFPVKVYFGHVQRLCGLGLDYLFLPRVVSVEPRSYICPKFMGVPDMIKAV
                     IPNLPTVIDMLVDLSKNRKVYDREVLRVGRYFTRDQVAINRAYRHGLEEQKKCEQICR
                     SGYSSSEAIALWEGGRLDLPERYDLQIVVLGHGYSLYDQTISMNLIQRLRNMGCRVHF
                     PEGGDHVKIEMAAATMPKRVFWTLGRKNVGAALHYAQQEQIDGMIYLACFGCGPDSMV
                     GDLIERKIKNKPFMMVTIDEHTGEAGMVTRLEAFCDMLRRRKETGHESDLSPHGEHVY
                     RPAGAADGTAAGICDAAADH"
     gene            9226..10323
                     /locus_tag="P5549_08000"
     CDS             9226..10323
                     /locus_tag="P5549_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA protein activase"
                     /protein_id="HRW13053.1"
                     /translation="MKVTFPHMGNMYIALQALLTGLQLEYVTPPLITKKTLELGVKYA
                     PEYVCMPMKINLGNFIEALELGADTIVMGGGWGPCRFGYYAQIERDILQELGYDFQMI
                     VLESQDSKATSLFRQLKALGQNVSLRQALHSVKFAWDKMTAVEQVEKGLEYYLPRASQ
                     PDAAEKIYVQGLNEIITAASGRQSLQIARQTIARMQSMELQAGDPLVIGLVGEVYTVL
                     EPAANYEIIQGLGRLGVEVRRTIYMTDWVNDHLLGGHVKPSTRAQVLNSARPYLNYWV
                     GGHGQETIGTTVAMARKGCDGIIQIGPLTCMPEIVAQTILPLVSEKEGIPCMTLWFDE
                     MSGTAGIQTRLEAFVDMVARRKHSTGQVKEA"
     gene            10326..11318
                     /locus_tag="P5549_08005"
     CDS             10326..11318
                     /locus_tag="P5549_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRW13054.1"
                     /translation="MDVYLGVDIGSVSSNFVFLDDQNRIMKKVYLRTAGNPVQAIRNG
                     FAQLNHADGGLCIKGVGTTGSGRYLAATLLGADAVKNEITAHAVAASHRYPAVRTIME
                     IGGQDSKIILIRNGIVHDFAMNTVCAAGTGSFLDQQAARLHIPIEEFGALAVRSSQPV
                     RIAGRCGVFAESDMIHKQQMGFPREDILMGLCEALVRNYLNNVGKGKKIEEPVVFQGG
                     VAANLGLKTAFEKVLGQTIHVPPDFEVMGAIGAAMLAREKIQSSAGNTVFRGAAFIAE
                     GEFRSTSMECQGCPNHCEVVSIKRDQQVLAYWGDRCKKWESSLKASGKNGLQVV"
BASE COUNT         2802 a         2700 c         3340 g         2507 t
ORIGIN      
        1 ccggacaatg tgctcgatcg aatcgttgcc gccggcatga aggtagccgg aatcggtaaa
       61 atccatgata tttttgccgg caaatccatc tccaccactg aacatattga aaacaaccgg
      121 gacggattgg ccaaaaccgt acaagctatt gccggagaag cctttagtgg actgcttttt
      181 gtcaatctgg ttgactttga ccagcaatac ggtcatcgca atgatgccca tggatatgcc
      241 ggcgcgctgg aggaatttga ccggcatctg agcgaggtta tggcggtcct gcaagccggg
      301 gatattttat ttattaccgc ggatcacggt tgtgacccca cgaccagcag caccgaccat
      361 tcccgtgagc aggttccctt gctggtctac ggacagccgg tgaaaaagga tgtcctgctg
      421 ccggtccgca cttcgtttgc cgatctgggc cagacgatcg ctgaatattt agggatagaa
      481 gcagactccc tgccgggaga gagcttttat gcgttcataa ggagatagca catgaacgtc
      541 atcgagatga taaccaaaaa acgtgacggc ggggagatga atcaagccga gatcgagttt
      601 atggtcaagg ccatcagcag gggttcgatt cccgactatc agatttctgc ctgggccatg
      661 gcggtctatt ttcagggaat gagtgatgag gaaacaaccg ccctggcgct ggccatggca
      721 tattccggag aagtgatgga cttgtccgcc ctcgaaggca tcaaagtgga caaacacagt
      781 accggcgggg tgggagacaa aaccacgttg gtggtcattc ccctggcagc ggcagccggc
      841 gtgccggcag ccaaaatgtc cgggcgaggt ctcgggcaca ccggcggcac cattgacaag
      901 tttgaagcca ttcctggttt ccaggtggag atgaaccggg aacaatttct gcaacaggtg
      961 gccaggatca aagcggcggt catttcccag agcggtaatc tggtgccggc ggacaaaaaa
     1021 ctatatgcca ttcgcgatgt taccgccacc atcgaaagca tccccttgat cgcttccagc
     1081 atcatgagta aaaagatcgc tgccggcgca cagggaatcg tcctggatgt caaggtgggg
     1141 cagggggcat tcataaagga ccgggtgcaa gcgctgcaac tggccgaaac catggtcagg
     1201 ataggacggg gcgcgggacg ccgggtggtc gccttgttga ccgacatgag ccagcccctg
     1261 ggtcgtttca ttggcaatgc cctggaagtc agggaagcca ttgacctgct ccaagggcag
     1321 gggccggacg acctgcgcca gctctgtatt gaactggctg ctcacatgtt ggtgctgggc
     1381 ggtcaagcgg acgagctgga gacagcccgg gagaaactgc gggtgttgct ggcctccggt
     1441 gcagccctgg ccaaatttga tgagctggtg gttgcccagg ggggacaact gcattataat
     1501 caacctgatt gcggattgcc ccaggccagt atcaaagaaa aggttgcagc tgacaggagc
     1561 ggctttgtca ccggactgga tgccttgaaa gtgggtcaca gtgccatgct gttgggggcc
     1621 ggacgggaaa ggctggacga tgtgattgat cctgctgcag gcattgaact gctgaaaaaa
     1681 tgtggagatt atgtacaagc cggcgaacca ctggccattt tacatgccaa ttgccaggaa
     1741 cgcctggcgc aggtgcggcc attgctgcgg gcagcctttc agctgggcgt tacggctgag
     1801 ggcattccac cgttgattta tcaaaccatc gaataagatg aaaaaacagg agatgaagca
     1861 aaatgacatg gcattcattt ttacccggac acatttattt tggcaacaag gtcatccaac
     1921 agaaccccga aatgtttcgc gggttgggtc gtcaggccct gatcgtaacc gggcaggggg
     1981 ggtctgccag acgcatcggc gctctggatg atatcaccca ggctctgcag cagcaagaca
     2041 tccaatggga aatcttcgat caggtggaag ccaatccttc cattgctacg gtacgtgacg
     2101 ggggccagcg cgcccggcaa agcggggcgg atttcatcat tgccatcggg ggcggctcac
     2161 cgatggatgc ggccaaagcc atcgcagtgc tggccttgca tgacatcagc gatgatcagc
     2221 tgctggcgct gcagttcggt gaagcgctac cgctggtggc agtaccgacg acagccggga
     2281 ccggatcgga agtaaccgcc gcttcgatta ttacctttcc cgagcgggaa gccaaactgg
     2341 gtatccgaga taacaagctg ttgccccgag tagccatcct ggaccctggc tataccgtct
     2401 ccctgccctg gcagattacg gcagatacag cagtggacgc ctattcccat gccatggaaa
     2461 gttatttgtc agtaattgct accccgatca gtgacatgca tgcccggacc gctttggata
     2521 ttttgggacc acagttaaag cggttaaccg cttcccggga aatcggcatg gccgaccggg
     2581 aagctctgct gctgggatcg ctgctggcgg gcatggccat cagtgaaacc agggtaacca
     2641 ttcctcattt gctggggtat accttcacca tatataaaga tatccctcac gggcgcgcca
     2701 atggcttgct gatgcccgct tttatgacgt ttaatctgga gaaaagccag gaccccaaag
     2761 taaaacaggt tctggaatac tcccacttca aggatttgca ggaattaacc gaggtgctag
     2821 cgcaactgtg cggtccgccg cccgtctgca gcgaagctga acggcagcac tttgttgatc
     2881 gtactaccgg caatcccagt ctggccaaca atctggttgc ccctgaccgg gaagatctgg
     2941 cagcgatgct ggccagagct ctgcagtaag ggcaggtttt gcgccggcct gacagcccca
     3001 agttggaata atctgctggc ctcttcaata tacttttaca aagttagggg ggaggtagct
     3061 gacagatgaa aaagaaatgc gcagtggcct gcattttaat ggtggttttt ttcttgggtc
     3121 tggctccgcc agtgctggcc ggtgctgaac tggaaaccgt taaagcagag gcctacataa
     3181 tgacggaggc tgattcaggc aaagtcttgc tggccaagaa tgaaaacaaa cgcctgccgc
     3241 cagccagcat gaccaaattg atgaccctgc tgctggctgc agaagccctg gaggagggac
     3301 gggttaccct caaggaaaaa gtgagcacca gtgatcacgc ttcccaaatg ggaggatcac
     3361 aaatttatct ggcaccggga gaggaaatga ccttcgaaga catgttgatt gctgtggctg
     3421 ttgggtccgc caatgatgct tcggtggccg tggcggaaaa gctggaagga acacacttgg
     3481 ccttcgttga caagatgaat gccaaggcca aagaactggg catgaaaaat acccaattca
     3541 agaattccaa cggcttgccg gcaacggatc attttacaac tgctgccgat atggctatct
     3601 tggcgcgggc cgctctccgg cagccgcggg tcatggaata ctgctccatc aaggaatatc
     3661 cgctgcgcgg cggcaagttt atcctctaca ataccaacaa gctgttgtgg tggtacaagg
     3721 gcactgacgg tttcaagacc ggacggacgg aagaagctaa aaactgtctg acctccacgg
     3781 taaaaagaga cggcttgcgg ctgattgctg tagttatggc atccccggag acccgtggcc
     3841 atttccgtga ttccatggag atgtacaatt acggttttgc ccgctatgct tacaaaacgt
     3901 ttgtaccccg gaataaaatg tgcgggacgg tcaaggtggg gaaaggccgg caagaacagg
     3961 ttgaggttct tgcccaggaa gatgccgggg cactctttgt caaaggagag aaagcaaaaa
     4021 ttaccagcaa acagatgttg cttaaatatg tcgatgcccc ggtcagcaaa ggcgaaaagc
     4081 ttggcgaagt tcagatttac aaaaatggaa ccaagataaa aacagtggct ttgattgctg
     4141 cccaaaatgt ggaaagaggc ggaatcttgc gacaaattaa aaaaatgttt gtagaaacat
     4201 atctattatg aatggaattt agtggaagga attactcccc tctctgtgga actagttttc
     4261 agtccagtat acagagaggg gacttttcat gaagattgag tgcaaacagg taagaagcac
     4321 gctggtcgtc aaactcagcg gcgaatttga tatgatgatt gcagacaaat tgcgccgtga
     4381 gatagacagt cgtttggaaa gcaagcaaat aaccaacctc atcatcaacc tggagaaagt
     4441 caccttcatc gacagctccg gtttgggagt gatcatcggc cgctacaaaa aaatcagttc
     4501 gcaaaaaggg catatgtaca tcgtcggtgc cagtgcctcg gttgagaaga ttttaacctt
     4561 cagcggcatc aataaattga ttcccatcta caagaatgaa caggacatca tcaacattta
     4621 aaggaggtgg agaccagctc caggctggaa tggctatgaa tataagaaat tatatgcgca
     4681 tcgaatttcc cagtcttccc gaaaatgttt cttttgcccg ggccagtgtg ggggcctttg
     4741 cggcccaact cgactgcacc ctggaggaaa ttgaggaagt caagctggtg gtctcggaag
     4801 cagtttccaa ctgtattatc catgcttatg acaatcgcag caacgaaaaa atcatggtga
     4861 tggccagcat cgggcttgaa cgtgaactgg aaatcctggt ggaggacacc ggcaagggca
     4921 ttgccgatat cagtcaggcc atggaaccca cctattccag cgacgagacc cggatgggaa
     4981 tgggttttac ctttatgaaa tcatttatgg acaacgttga agtaaggagc atagttggag
     5041 agggtaccag tgttacgctt cggcgcactt ttttgtacca tgacaaagaa gcgttagctt
     5101 aagcaagggg tgatgagttt gcccctggaa aacaaatacg atgacaatga aaagatggta
     5161 gaacgggccc aaaacggtga tgcccaggcc cgggcgcaga tatatgaaaa caacctcggg
     5221 ctggtctata tggtgctgga acgatttaaa aacagttcct atgaatacga agatttattt
     5281 caaatcggct ccatcggtct gttaaaggcc atcgaaaaat ttgattttgc ttaccaggtt
     5341 cgcttttcca cctacgcagt gccgatgatt atcggtgaaa tcaagaaatt cctgcgtgac
     5401 gacggaatcg tcaaggtaca gcggggcctc aaggagacgt atggcaaagt gcgctgggcc
     5461 caggataaac tgcgcggcga cctggggcgt gagccgggga ttgcggagat tgccagtttg
     5521 ctggagattg acaaggagga aatcgtgctg gccatggagg cctgtcaatc acccgcctat
     5581 atgcaggatg tcatgccggg agaaaaggac cagttgtccc tcatcgacaa gctggccaat
     5641 gaagatggca atgttgtgtt gctggaacag atggccttgc gcgaagccct ggccaaactc
     5701 gaggaacggg agagggaagt actgctcaga agatacttca aggatgaaac ccagatggtc
     5761 attgccgaag atctgggggt atcccaggta caggtatcgc ggattgaaaa agctgctctg
     5821 cgcaaactga aaaatttgct tgcataaaaa atcgttgcct gcaacagaat atataaccag
     5881 agaggagaaa gtgtttgcct ggacagatgc cttctccttt atttttgcaa agggggaaag
     5941 aatatgcaga tcaaagtggc agagatcgtc ggcgaatcca aaaaggactg gcaggatgcc
     6001 gtcaagaacg cggtcaagga agcgtcccgg gaaatgggca atatcaccgg agtagaagtg
     6061 tataattgga cagccgattg ccaggacgac cagattatcg aatacaaggc caacatcaaa
     6121 attgcttata cggattaaag cgtttctctc aacaatgaac aaatcggaat gacttcgtgt
     6181 ctgcctggca ggcgcgaagt tatttttctt gtgggggaga attttgggga tggcaacagg
     6241 agacttgagt aaagccctgt cgccggaggt cggttaaagg ctgcgcttct tcgggctgaa
     6301 taatctctcc ggggatggtt cataatatgt tagcaagggg ggaagcagca tgcaggaggc
     6361 caagagcgaa gaactaaagt ctctcgagaa aaaggaatat caaaacctgg ttaatcaggt
     6421 gaaacccaaa ccgcctttac tgaaaaactt tttatgggcg tttggcgtag ggggattcat
     6481 ctgtgtgatc gcccaaatgc tgctcaatta tttgctcttc cgcggaatga gtgagaccga
     6541 tgccgggtcc tcggtggcag tcgtcatggt ttttctgggg gcgctgctga ccggaatcgg
     6601 cgtttatgac aaaattggag aaaaggctgg tgccgggtcc atcgtaccca tcaccggttt
     6661 tgccaatgtg attgttgctt cggccatgga gtttaaacgg gaaggatata tcttcggcgt
     6721 aggggcccga atctttagta ttgccgggcc gacactgttg ttcggcttca cggcatcagt
     6781 gttgatcggg ctgatcagtt attggctgaa ataggcagcg aaagggaaaa aacatgacag
     6841 cggcgagaaa acagggacaa caaagcgtta tatttgccca tcctccggtg ctcagtgcct
     6901 gggcatcgat tgtcggaccc aaggaggggc aggggccatg ggcccgggac tttgacaaga
     6961 tcctggatga ctatttggat gggcaaagca gttgggagaa ggcggagaac aaattgctgc
     7021 agaccaccgt aaagacagcg gtggccaaag ccggactgga tatcaccgaa gcggaactgc
     7081 ttctggccgg tgatctgctc aaccagatca tttctgccaa ctatgccgcc cgggaattaa
     7141 atgttccctt tttaggcttg tatggggcat gttcgaccat ggccgagtcg ctgctgctgg
     7201 ctgccatgct ggttgatggc ggtttttttg aacgggtggt ggccgccacc tgcagtcatc
     7261 actataccgc cgagcgacag tatcgtgcgc cggtggagca gggcgttcaa ccgtctccca
     7321 gcgcccaatg gacggcgacg gcttccggtg cggtgctggt acagccaggc ggcaatggcc
     7381 cgcgtattac ctgtgccacc atcggcaagg tcgttgacat gggcattact gatgctgcca
     7441 atatgggcgc ggccatggct ccggcggcag gcagcaccat ccaggcccat tttgcagata
     7501 caggcaggcc aagcgactat tatgatctgg tggtgaccgg cgatcttggc acggtgggca
     7561 tgaagctgct caagcggatt tttgtccagc atggaatagt gcctcagcct gtctacagcg
     7621 attgtggcgt actgatctat gacgggctgc caaatgtgaa agccggtgcc agcggctgtg
     7681 gctgttcggc tgccatgctg tgcgggccgc tgctgcaaaa actcagccgg ggagagatta
     7741 accggctcct gtttgtggcc accggagcgc tgatgagtcc catcagtacc tttcagggtg
     7801 aaagcattcc cggcattgcc catgcgctgg cgattgaaaa cacctagtgc aaacagcgaa
     7861 ttacttacat tatggaaggc ggctgcaata tgtcatacgt aacggctttt ttgctgggcg
     7921 gatgcctgtg tgtcatcggc cagctggtaa tggatctgac caagcccggc attacccccg
     7981 gtcatgtgct ggtggggttt atcaccggcg gagccatatt gagcgcggtg gggctttatc
     8041 agccgctggt cgattggggg ggagcgggtg ccaccatccc tttgagcggc tttggccaca
     8101 gtctggccca gggggccatc gacggtgccc gggaaaaagg cctgctgggt gtcttcagcg
     8161 gcggcgtggc agcgactgca gcagggatta cagcggccat cgtgtttggt tatctggcag
     8221 cggtgatctt taaccccaaa gattaggcaa ggaggaagaa aatgcagcga gtcggcattc
     8281 cccaaggact tttttaccat tattactatc ccttgtggaa aacctttttt acagatctgg
     8341 gcgcggaggt ggtgagttcc ggtccgacca accgcaccac ggtggagcag gggatcgccg
     8401 ctgcagtgga tgaaagctgt tttccggtca aagtttactt cggccacgtc cagcgcctgt
     8461 gcggtctggg ccttgattac ctgttcctgc cccgggtggt cagtgttgag ccgagaagtt
     8521 atatatgtcc caaattcatg ggcgtgcctg acatgatcaa agccgtaata cccaatctgc
     8581 cgactgtcat cgatatgctg gtcgacttga gcaaaaaccg caaagtgtat gaccgggaag
     8641 tcctgcgggt gggacggtat tttacccggg accaggtggc gatcaatcgg gcttatcgtc
     8701 acgggttgga agagcagaaa aagtgtgaac agatctgccg cagcggctac agcagcagtg
     8761 aagccattgc cttatgggaa ggaggccggc tcgatctgcc ggagcgttat gatctgcaga
     8821 ttgtggtgct ggggcacggg tattcattgt acgatcagac catcagcatg aatctcattc
     8881 agcgtttgcg caacatgggc tgccgggtgc attttcccga gggcggcgat catgtcaaaa
     8941 ttgaaatggc ggcggccacc atgcccaaac gggtattttg gaccttggga cgtaaaaacg
     9001 taggggcagc gctgcattat gcccagcaag aacaaatcga cggtatgatc tatctggctt
     9061 gctttggttg tggccccgat tccatggtag gagacctgat cgaacgaaaa attaaaaaca
     9121 agccctttat gatggtaacc atcgatgaac acaccgggga agccggcatg gtgacccggc
     9181 tggaggcatt ctgtgacatg ttgcgcagaa ggaaggagac tggccatgaa agtgaccttt
     9241 ccccacatgg ggaacatgta tatcgccctg caggcgctgc tgacgggact gcagctggaa
     9301 tatgtgacgc cgccgctgat cactaaaaag accctggaac tgggggtcaa gtatgcaccg
     9361 gagtatgttt gcatgcccat gaaaattaat ctgggcaatt ttatcgaagc tctggaattg
     9421 ggggcggata cgattgtaat gggcggcggt tggggaccct gccgttttgg ctattatgcc
     9481 cagatcgaac gggatatcct gcaggaactg ggatatgatt ttcaaatgat tgtgctggag
     9541 tctcaggatt ccaaggccac gtctttgttt aggcagttaa aagcgctggg tcagaatgtc
     9601 agtctgcggc aggcgctgca cagtgttaaa tttgcttggg ataaaatgac tgcggtagag
     9661 caggtggaaa agggtttgga atactatttg ccccgggcca gccagccgga cgcagcagaa
     9721 aaaatctacg tgcaggggct aaatgagatc attactgctg ccagcgggcg gcagagcttg
     9781 caaatcgccc ggcaaaccat agcccggatg caaagcatgg agttgcaggc cggggacccg
     9841 ttggtcatcg gtctggtggg ggaagtgtat accgtgctgg aaccggccgc caattatgag
     9901 atcattcaag gattgggacg gttgggggtg gaagtgagaa ggacgatcta tatgaccgac
     9961 tgggtcaacg atcatctgtt gggcggacat gtcaagcctt ccacccgcgc tcaggtgctg
    10021 aacagtgccc ggccttatct gaattactgg gtaggcggac atggccagga aacgattggc
    10081 accacggttg ccatggcccg caagggttgc gacggaatta ttcaaatcgg gcccctgacc
    10141 tgcatgccgg aaattgtggc ccagaccatt ctgcccttgg tcagtgaaaa agaaggtatc
    10201 ccgtgtatga ccctctggtt tgatgaaatg agcggtacgg ccggtattca aacccggctg
    10261 gaagcatttg ttgacatggt cgcccgccgc aaacacagca ccggtcaggt aaaggaggcg
    10321 taatattgga cgtatatttg ggggtggata ttggctccgt cagcagcaat tttgttttcc
    10381 tggatgatca aaaccgaatc atgaaaaagg tttatctgcg tactgccggc aatcctgtcc
    10441 aggcgatccg caacggtttt gcgcagctga accatgcaga cggcggactg tgtatcaaag
    10501 gcgtgggcac taccggcagc gggcgctatc tggccgctac cctgctgggg gcggatgcag
    10561 tcaaaaacga gattactgcc catgcggtag ccgcctcgca caggtatcca gcagtgcgca
    10621 ccatcatgga gattggcggg caggattcca aaattattct gatccgcaac ggcattgtcc
    10681 atgattttgc tatgaacact gtttgcgctg ccggtaccgg atcctttctc gaccagcagg
    10741 cagcgcgttt gcatattccc attgaagaat ttggtgcctt ggcggtccgc tcctcccaac
    10801 ctgtcagaat cgcaggtcgc tgcggcgttt ttgccgagtc ggatatgatc cacaaacagc
    10861 aaatgggttt tccgcgggaa gacatcctga tggggttgtg tgaagcgctg gtcagaaact
    10921 atctgaacaa tgtcggcaaa ggtaaaaaga ttgaagaacc ggtggttttt cagggcggcg
    10981 tcgctgccaa cctgggcttg aaaaccgctt tcgaaaaagt gctgggacag acaatccatg
    11041 tgcccccgga tttcgaagtg atgggtgcca tcggtgctgc catgctggcc cgggaaaaaa
    11101 tccagagcag tgctggcaac accgtttttc gcggtgccgc ctttattgcc gagggagagt
    11161 ttcgcagcac cagtatggaa tgccagggat gtcccaatca ttgtgaagtg gtcagtatca
    11221 aaagggacca gcaagtcctg gcttactggg gggatcgctg caaaaaatgg gaaagcagcc
    11281 tcaaagcaag cggcaagaat ggcttgcagg tcgtttagtc aggcgacggg gtttcctgca
    11341 gcatcgtca
//
LOCUS       DAOTHZ010000066        11257 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_6878_length_11257_cov_7.224960, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000066 DAOTHZ010000000
VERSION     DAOTHZ010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 11257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11257
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6878_length_11257_cov_7.224960"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="P5549_08010"
     CDS             complement(<1..187)
                     /locus_tag="P5549_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13055.1"
                     /translation="MELLAPAGNWEAFLAAMRNGADAVYMGGKNYSARQSAENFDLEQ
                     IGRAVEYAHLRGKKVYMT"
     gene            complement(209..1180)
                     /locus_tag="P5549_08015"
     CDS             complement(209..1180)
                     /locus_tag="P5549_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HRW13056.1"
                     /translation="MKLAEIYRLAVQLGKESDIRGDYLQHLLQENQRHFGQMTAQEQY
                     FFDSQSLENPFNDTRILVGSGDEEIGSILCGIDMETQELLLADRLRERGRRIDMVLAH
                     HPEGIAEAELHEVMQMQADMLEKMGVPINIGEGLMASRMGEVRRGRMPLNHQRAVDAA
                     RLLDIPFMCTHTVADNLGSRFLYERLTEAEVVTVDDIMDLLLEIPEYIEARRLKAGPA
                     MIAGNGKRRAGKVFVKFSGGTATSEKAYEQLARAGVGTIIGMHMPEKHRAAARENHIN
                     VIIAGHMASDSLGMNLLLDEIESRGVEVIACSGLIRVRRFIVDEIDD"
     gene            complement(1224..1898)
                     /locus_tag="P5549_08020"
     CDS             complement(1224..1898)
                     /locus_tag="P5549_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRW13057.1"
                     /translation="MISLLGFTLRVAGMYFLAMIMIRILGKRALGELGPFDFVVMTGV
                     GDTVISVALNKNMPLTDGIIVLATLAFLEYAVGFLSLKSGRLSNIITGKPVVLIENGK
                     IIRKNLAREKFNVDDLLQELRKRGVREIGEVERGILEACGGFSVILKEGEETVRRKDL
                     GLKKQPASSLLTIDPIPRNEIFAQLKQEVDEEPGYDLLAGMANIETQLQQLNERMSVM
                     EQYVKS"
     gene            complement(1927..2052)
                     /locus_tag="P5549_08025"
     CDS             complement(1927..2052)
                     /locus_tag="P5549_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13058.1"
                     /translation="MIPRLQVDDTPAISRVELFNRNSSIFYAPRQKNKAVPAAEY"
     gene            complement(2094..2831)
                     /locus_tag="P5549_08030"
     CDS             complement(2094..2831)
                     /locus_tag="P5549_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRW13059.1"
                     /translation="MTTQFKLHNVVKRYGSRTVLTIDQLALPEGRIYTIMGPNGSGKS
                     TLLRIMGLLLVNDEGELEVLGEKVNWEKEQLLQLRRQMSMVTQTAFMFQGSVEYNVAY
                     GLKARNMKSRLIRARVGEMLEMAGMSAYREADARTLSGGERQKVALARAMAVKPKVLF
                     LDEPTSNIDMASAAEIEKYIRAINQQQGTTIILVTHNLFQARRLADEVIFLHEGHIIE
                     RGSAQFIFDDPRDQRTLAFLHGEAVMQ"
     gene            complement(2806..3525)
                     /locus_tag="P5549_08035"
     CDS             complement(2806..3525)
                     /locus_tag="P5549_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW13060.1"
                     /translation="MNIFVEGIKEGVYLIWQMDPEIIQVTLLSLQVSLTALLLSAMLG
                     IPTGAALALKKIPGKRIWLNTIYTLMGLPPVLAGLLVYLLLTYRGPLGQFELLFTPSA
                     MVFAQVLLAAPIVCGLTARAVMALPQDVYDTAATLGASRRQASATIMREARIGIVAAL
                     TTAFGRVIAEVGAVMLVGGNIRWSTRVLTTSIVLETRMGNFSTAIAIGLILLSLSFLI
                     NLLILKLEKRTLRYDNTIQIT"
     gene            complement(3564..4424)
                     /locus_tag="P5549_08040"
     CDS             complement(3564..4424)
                     /locus_tag="P5549_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRW13061.1"
                     /translation="MRNHKTLLVLAVLMAFLFTMVGGCAQKEPPAPEQSQQPPAKELK
                     LATTTSTQDSGLLDAILPELEKDINYKVKVIAVGTGQAIEMGEAGDVDVILVHSRAAE
                     DKFVADGYGVDRRDVMYNDFLIIGPAEDPAGIKGSTDIIAAFKTIADKQAVFVSRGDN
                     SGTDKKEKGIWTKAEMTPAGAWYIETGQGMGDTFRMADEKKAYTLIDRATYLAQKDKY
                     KLEPIIEGDKSLLNPYGVIPVNPEKFPNVDYEGATKFAQWLTSEKGQKMIGEFGVKEF
                     GQQLFVPDAK"
     regulatory      complement(4508..4624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4813..5835
                     /locus_tag="P5549_08045"
     CDS             4813..5835
                     /locus_tag="P5549_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW13062.1"
                     /translation="MKSGSITQKSLVTLFILLFSLTLLAGCGQKDQPAAEDQAVQFKI
                     GHCTWVGYAPLYIAQEKGFFKNHNIAPEMVIIEDESQYAAAITANQIQALGNVVDREI
                     IHFANGAPIKFVLGMDQSYGGDGIIASAQISAPKDLEGKTVGLDKASTSYFFFLAVLD
                     KYGVDESKIDIQEMTAGDAGAAFVAGKLDAAVAWEPWLTQASQREGGHVLVSSKDFPG
                     YIVDVISVRQDFIDAHPEAVQGLVEAWNEAIAWYQQNPDEGNEIMAKALALSKEEVAD
                     MAKGVQFMGKEENRDFFNAALSENTLYSLTGMASRFWLDKGVIKEAVDARQIIDPTFV
                     EAATAK"
     gene            5949..6737
                     /locus_tag="P5549_08050"
     CDS             5949..6737
                     /locus_tag="P5549_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW13063.1"
                     /translation="MNLYHNSKSKNQELLTWQIVSLVLIASAWCLLSFGFKINPVFLP
                     TPLAVAEAFRELWQEGVLLSYTLISLYRVLIGFFAAAIIAIPLGILMATNTKTEGFFA
                     PFIGFVRYLPVSALIPLMILYFGIGDFEKMAVIFVGTFFQLVLMVQDTVGTVPGDLLK
                     AACTLGADGSAIYTKVLLPASMPGIMDNLRICIGWAWTYLVIAELVAANSGLGFMILR
                     SQRFLQTDRIFAGLIIIGCLGLLTDYLFKTLSKVLFPWYERLGQ"
     gene            6738..7511
                     /locus_tag="P5549_08055"
     CDS             6738..7511
                     /locus_tag="P5549_08055"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13064.1"
                     /translation="MLLFEGVYKTFSSGDKRVEALEDINMQVEQSEFAVIVGPSGCGK
                     STLLQIAAGLEHPTAGKAYLNDTEITGPGADRGMVFQAYTLFPWLNVADNVAFGPRQK
                     KMPEQEVQERVNRYLEVTGLLKFAKLYPKALSGGMKQRVAIARALANDPEVLLMDEPF
                     GALDAQTRVVMQELLLQIWETDHKTVLFITHDIDEAILLADTVHVMSRQPGSIKKTLS
                     IPLARPRDHSLTLRPEFIGLKKEILELIWEESKAAAQEF"
     gene            complement(7624..9099)
                     /locus_tag="P5549_08060"
     CDS             complement(7624..9099)
                     /locus_tag="P5549_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRW13065.1"
                     /translation="MTKNGLQIAKGENELYILPSMANRHGLIAGATGTGKTITLKVLA
                     ESFSATGTPVFLTDIKGDLAGMCEPGEDSPKMQERIKKLELDNFNYHAFPVQFWDVFA
                     RQGHPVRTTISEMGPLLLSRLLGLNDTQTGVLNIAFKIADDQQLLLLDLKDLKTMLVY
                     VAENAAEYTTEYGNVSKQSIGAIQRAILVIEEQGADQFFGEPALDIYDLLKTDRDGMG
                     YINIMACDQLFHSPLLYSTFLLWMLSELFEELPEVGDLDKPKMIFFFDEAHLLFNDAP
                     KVLLERIEQVVRLIRSKGVGIYFITQNPADIPDSVLGQLGNRVQHALRAFTPKDQKAI
                     KAAAETFRQNPNYKVADVITELAVGEALVSFLEEKGSPAMVQRAFILPPRSLMGPISE
                     QRRSQAINQSIMYGKYEKTVDRESAYEKLQAKMLREKEEEQRQEEQAAAEKERQAASR
                     SKPGRKSDTFVEQMTKSAVNTVGRQIGRELVRGLLGSLLKR"
     gene            complement(9132..9416)
                     /locus_tag="P5549_08065"
     CDS             complement(9132..9416)
                     /locus_tag="P5549_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRW13066.1"
                     /translation="MDAKKNTVNKVTVSIYNEDYVVKGNEDPEYIQMLGSYVDRRMRM
                     IGQRNHHLSTSKVAVLTALNLADELNKLQEDYDQLIKALEEERKNRLGQS"
     gene            complement(9677..10345)
                     /gene="phoU"
                     /locus_tag="P5549_08070"
     CDS             complement(9677..10345)
                     /gene="phoU"
                     /locus_tag="P5549_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRW13067.1"
                     /translation="MVVDNLDIELKKLKEEILKMGRLLEEQIFKAVKSLVDKDIEMAQ
                     EVIERDDIMDAMAVEIENKCLQLIALKQPMARDLRFIGTALRIIVDMERMADHAEDIG
                     EITIRLHDQKYIKPLIDIPRMAETAEGMVRIALKAYVDGDVDMAMSLVGMEKLSDELY
                     EQVYRDLLLHMMQDARNIPQATDLLLVAGHLERIADHATNLAEMVVYNEEGKRIDINV
                     LARA"
     gene            complement(10374..11135)
                     /gene="pstB"
                     /locus_tag="P5549_08075"
     CDS             complement(10374..11135)
                     /gene="pstB"
                     /locus_tag="P5549_08075"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRW13068.1"
                     /translation="MEKTLKMQSQNLQVFYNQYQALQGINMDIEENKVTALIGPSGCG
                     KSTFLRSLNRLNELIEGTTIKGQIYLDQQPIYSSDVDVVALRKKVGMVFQKPSVFPMS
                     IYDNVAYGPRIHGIKNKSRLDEIVEKSLKAANLWKEVSDRLKSPAARMSGGQQQRLVI
                     ARVLAVEPEVILMDEPASALDPISTTRLEELIGELKNHYTIVIVTHNMQQAARVSDYT
                     GFFLSGELIEFGKTTEIFVNPLDKRTEDYITGRFG"
BASE COUNT         2657 a         3007 c         2593 g         3000 t
ORIGIN      
        1 ccgtcatata tacttttttg ccgcgcaaat gggcatactc cactgcccgg ccaatctgct
       61 ccaggtcaaa gttctcggct gactggcggg cgctgtagtt ttttccgccc atatagacgg
      121 cgtcggcgcc atttctcatc gccgccagga aagcttccca gtttcctgcc ggggctaata
      181 attccatttg attcacataa cctccggatc aatcatcgat ttcatcaaca ataaaacgtc
      241 tcacccggat gagacctgaa cacgcaatga cttccacccc tctgctttcg atttcatcca
      301 gaagcagatt catccccagg gaatcactgg ccatatgtcc ggcgataatc acattgatat
      361 ggttttcccg agcggcggcg cgatgtttct ccggcatatg catgccaatg atggttccaa
      421 cccccgcacg ggccagttgc tcgtaggctt tttctgaagt cgctgtgccg ccggagaatt
      481 tgacaaaaac ctttccggct cgtcgtttgc cgttgccggc gatcatcgcc ggacctgcct
      541 taaggcgacg cgcttcaata tattcgggga tttccagcag cagatccatg atatcatcca
      601 ccgtaacgac ttccgcctcg gtcagccttt catacaaaaa acggctgccc aggttgtcgg
      661 ccacggtgtg cgtacacata aaagggatgt ccagcagccg ggctgcgtcc accgcccgtt
      721 gatggttaag tggcatccga ccccgccgca cttcgcccat ccggctggcc atcaaacctt
      781 ccccgatgtt gatggggaca cccatttttt caagcatatc cgcctgcatt tgcatcacct
      841 catgtagttc agcttcagcg atcccctcgg gatggtgagc caaaaccata tcaatgcggc
      901 ggccccgttc gcgcagacga tcggccagca gcagttcctg ggtctccatg tcgataccgc
      961 ataaaatgct gccgatctct tcatctccgc tgcccaccaa aatccgggta tcattgaagg
     1021 ggttttccaa tgattgtgaa tcaaaaaaat attgctcctg tgcggtcatt tgtccaaagt
     1081 ggcgttgatt ttcctgcagc aagtgctgca gataatcgcc ccggatatca ctttcttttc
     1141 ccaactgcac cgccaggcga tagatttcgg caagcttcat aacaacctcc gcttttttgt
     1201 cattattata acacagagtg gatttagctt ttaacatact gttccatgac actcattcgt
     1261 tcgttcagtt gttgcaattg cgtttcgata ttggccatcc ctgccagcaa atcatagcct
     1321 ggctcctcat cgacctcctg cttcaattgg gcaaaaattt cattgcgggg gatgggatca
     1381 atcgtcagca aggaactggc gggttgtttt ttcaatccca aatccttgcg tctgacagtt
     1441 tcctcccctt ctttcaagat gacgctgaag ccgccgcaag cttccaggat acccctttcc
     1501 acctcaccga tctctcgcac ccctcttttg cgcagttcct gcaacaaatc atcaacgttg
     1561 aatttttccc gggccagatt ctttctgata atcttgccat tttcaatcaa aaccaccggt
     1621 ttgccggtga tgatgttgga tagccgtccg cttttcaagc tcaaaaaacc taccgcatat
     1681 tccagaaaag ccagcgttgc cagaacaatg atcccgtctg tcagcggcat gttcttgttc
     1741 agcgcaaccg aaataaccgt atcacccacc ccggtcatga ccacaaagtc aaacggtccc
     1801 agttcgccca gcgcccgctt gcccaagatg cgaatcataa tcatggccag aaaatacatt
     1861 cctgccactc ttaaggtaaa gcctaacaaa ctgatcatgg cataaccccc tgacttgatc
     1921 acatgtttag tattcagccg ccggcaccgc tttattcttt tgtcttgggg cataaaaaat
     1981 agagctgttc cggttgaaca actccacccg agagattgcc ggggtatcat ccacctgcaa
     2041 tctcggaatc attgccataa aatctccgct ggttgcctgc ctggataaga aaactattgc
     2101 atgaccgctt ctccgtgcag aaatgccagg gtcctctgat cacgcgggtc atcaaagatg
     2161 aattgggcgc tgcccctctc aatgatatgc ccctcatgca ggaatatcac ttcatcagcc
     2221 aaacggcggg cttgaaagag gttgtgggtt actaaaataa tggtcgttcc ttgttgctga
     2281 ttgatggcac gaatatattt ttctatttcg gctgcactgg ccatatcaat gttggaagtc
     2341 ggctcatcca ggaacaatac ttttggtttg actgccatgg cccgggccag cgccaccttc
     2401 tgacgttctc ctcccgacag ggttcgggca tccgcttccc gataggctga catgccggcc
     2461 atttcgagca tttcaccaac ccttgcgcgg atcaggcgag atttcatgtt acgcgccttt
     2521 aatccgtaag ccacattgta ctctaccgag ccctggaaca taaaggccgt ctgcgtcacc
     2581 atggacatct gccgccgcag ctgcagcaac tgctcttttt cccagttgac cttctcgccc
     2641 aacacttcca gttccccttc atcattaacc agcaggagcc ccatgatgcg cagcaacgta
     2701 cttttgccgg aaccgttggg gcccatgatc gtatagatcc tgccttccgg cagcgccagc
     2761 tgatcgatgg tcagtaccgt ccggctgccg taacgcttga cgacattatg taatttgaat
     2821 tgtgttgtca taacgcaagg tcctcttttc cagctttaag atcagcaagt tgatcaggaa
     2881 tgacaagctc aataaaatca aaccaatggc aatcgcggta ctgaaattgc ccatccgggt
     2941 ctccagcacg atggaggttg tcaacacccg ggtactccag cgaatgttgc cgcccaccag
     3001 catgaccgct ccgacttcgg caatcacccg accaaaggca gtagtgagag ccgccacaat
     3061 gccgatgcgt gcttcccgca taatcgtcgc tgacgcctga cgccgggacg ctcccaacgt
     3121 agcagcggtg tcatacacat cctgtggcaa cgccatgacg gctcgcgccg ttaacccgca
     3181 aacgatcggc gcggccagca aaacctgggc aaaaaccatg gcagacgggg taaacaacag
     3241 ctcgaactga cccagtgggc cgcggtaggt taaaagcaaa tacaccaaca ggcctgccag
     3301 caccggcggc agacccatca gtgtataaat cgtgttcagc cagattcttt tcccgggaat
     3361 ctttttcaag gccagggccg cgccggtagg aatacccaac atggcggaaa gaagcagggc
     3421 ggtgagggat acctgcaagg acagaagggt aacctggatg atttcgggat ccatttgcca
     3481 gatcagataa acgccttcct taatgccctc tacaaatatg ttcacgccca acctcccgga
     3541 atagtttcat tacattgcag caattatttg gcgtccggta caaacagctg ctggccaaat
     3601 tcttttacgc caaactcgcc gatcatcttc tgcccttttt cggatgtcag ccattgggca
     3661 aacttggttg ccccttcata atccacattg ggaaatttct ccggattgac cgggatgacg
     3721 ccataaggat tgagcaggga cttgtcgcct tcaattatag gttccagttt atatttatcc
     3781 ttctgcgcca gataggtggc tctgtcaatc aaagtataag cctttttctc atcggccatg
     3841 cggaaggtgt cacccatgcc ttgtccggtc tctatatacc atgctccggc gggggtcatt
     3901 tctgccttgg tccaaatgcc tttttccttt ttatcggttc ctgaattgtc acccctggat
     3961 acaaatactg cctgtttgtc agcaatggtc ttgaaagctg ctatgatatc ggtactgcct
     4021 ttgatgccgg ccggatcttc cgccggtccg atgatcagaa aatcgttgta cataacgtcg
     4081 cgccggtcta cgccatagcc gtctgctaca aacttgtcct ctgccgcccg ggagtgtacc
     4141 agaatcacgt cgacgtctcc ggcctcaccc atttcaatgg cttgtccggt gccaaccgcg
     4201 atgactttga ctttgtagtt aatatctttt tcaagctccg gcaaaattgc atccagcagt
     4261 cctgaatcct gggtgctggt ggtggtcgcc agtttcagtt ccttggccgg aggctgctgg
     4321 ctttgttcag gagccggggg ctctttttgg gcacatccgc ccaccatggt aaacagaaac
     4381 gccattaaca cagccaatac caataatgtt ttatggtttc tcattttcat tccaatccct
     4441 cctctaatca tagccataaa aaagggttct gtctgcagat tacaaaccag aaccatatat
     4501 caattttatt tctttcatct catctccttt ccagcgggag gcgtatccgt ttcccgatac
     4561 accctatcgg accatcaacc atagagattt ccttaggtca ataatctcgg agagtatttt
     4621 attactttga tattattgcc attatgcatc aatcccatta ccttgtcaat ttccagctgc
     4681 ttgatcagca aattcgattt accatggtga aggattaaca gtatagagat ttaaaccaga
     4741 gaaggatttt gcatattatt ggcgaataca tcattgaaac tttaagaaat catgcattgg
     4801 gaggtcaaaa aaatgaagtc aggcagtatc acacaaaaat cattggtaac gctctttatt
     4861 ctgttgttca gtcttactct gctggccggc tgcggacaaa aggaccagcc tgccgctgaa
     4921 gaccaggcgg ttcagtttaa aataggacat tgcacctggg ttggctatgc tccactatat
     4981 atcgcccagg aaaaagggtt ctttaaaaac cataatatcg ccccggaaat ggttattatc
     5041 gaagatgaat cccagtatgc agctgccatt accgccaacc agatccaggc gctgggcaac
     5101 gtggtcgacc gcgagatcat tcatttcgcc aatggtgcac cgatcaaatt tgtgctgggt
     5161 atggatcagt cctatggcgg cgacggcatc attgcctcgg cacagataag tgcccccaag
     5221 gatttggaag gaaaaactgt tggtctggac aaggcttcaa ccagttactt cttcttcctg
     5281 gccgtgctcg acaagtatgg tgtcgatgaa agcaaaatcg atattcagga aatgactgcc
     5341 ggcgatgccg gggcagcctt tgtggccggc aaactggacg ccgccgtcgc ctgggaaccc
     5401 tggctgacgc aagcttccca acgcgagggc ggccacgtac tggtatccag caaggatttt
     5461 cccggttata ttgtcgacgt aatctcagta cgtcaggact tcatcgatgc ccatccggaa
     5521 gcggtgcaag gcttggtgga agcatggaat gaagccatcg cctggtacca gcagaatcct
     5581 gatgaaggca atgaaatcat ggccaaggct ttggcgctga gcaaggaaga agttgctgat
     5641 atggctaagg gagttcagtt tatggggaaa gaagagaatc gtgatttctt caatgccgcg
     5701 ctctccgaga acacgctcta ttctctcacc ggcatggcta gccgtttctg gctggacaaa
     5761 ggtgtgatca aggaagcggt cgatgcccgg cagattattg acccgacctt tgtggaagca
     5821 gcaacagcaa aatagaggtg ttgctcgtca gacagagatg atttttcgat tcagcggcca
     5881 gattttctgg ccgttgattg tcgtttaagt gtataatcaa aaaggtattc cagttggggg
     5941 ggaccttttt gaatctctac cataacagca aaagtaaaaa tcaggaatta cttacctggc
     6001 aaattgtttc cctggttctt atcgcctccg cctggtgtct gctctctttc ggtttcaaga
     6061 ttaatccggt cttcctgccg acaccattag ccgtcgcaga agcgtttcgg gaactgtggc
     6121 aggaaggcgt tttgctctcc tacaccctga taagccttta tcgggttctg atcggcttct
     6181 ttgccgcagc catcatcgcc attcctttgg gcattttaat ggcgaccaac accaaaaccg
     6241 agggtttttt tgcccctttc atcggctttg tccgttatct gcccgtttct gcattgattc
     6301 ccctgatgat tctctatttt ggaatcggtg attttgaaaa aatggcggtc atctttgtcg
     6361 ggaccttttt tcaactggta ctgatggtgc aggacaccgt aggcactgtt cccggcgatt
     6421 tactcaaggc tgcttgtact ttgggagcgg acggttcggc catctatacc aaagtcctgt
     6481 tgccggcctc catgccagga ataatggaca atctgcgcat ttgtatcggc tgggcctgga
     6541 cctatctggt cattgcggaa ctggtcgccg ccaattcagg actgggattc atgattttgc
     6601 gttcccagcg tttcttgcag acggatcgta tttttgccgg cctgatcatc atcggttgtc
     6661 tgggtctgct gaccgattat ttgtttaaaa ccctgtccaa agttcttttt ccgtggtatg
     6721 aaaggttggg acaataaatg ctgttatttg aaggggtata taaaactttt agcagcggag
     6781 ataaaagggt cgaggcgttg gaagacatca acatgcaggt cgaacagtcc gagttcgctg
     6841 ttattgtcgg tcccagcggc tgcggaaaaa gcactctgct gcagatcgct gccggcttgg
     6901 aacatcccac cgccggcaaa gcgtatttaa acgatacgga aattaccgga ccgggtgctg
     6961 accgaggcat ggttttccaa gcctatacgc tgtttccttg gctgaatgtg gcagacaacg
     7021 tggctttcgg tccgcggcaa aagaaaatgc ccgagcagga agtgcaggaa agggtcaatc
     7081 gctatctgga agtaaccggc ctgctgaaat tcgccaaact ttacccaaaa gcattgtcgg
     7141 gcgggatgaa acaacgggtg gccatcgcca gagcgttggc caatgacccg gaggtgctgc
     7201 tgatggatga gccgttcgga gccctggatg cccagacgcg cgtggtcatg caggaattgc
     7261 tgctgcaaat ctgggaaacc gatcacaaga cagtgctttt cattacccac gatattgacg
     7321 aagcgattct gctggccgat acagtgcatg tgatgagccg acagccgggc agcatcaaaa
     7381 agacgctctc cattcccctg gcacgtcccc gcgatcacag cctgacgttg cgtccggaat
     7441 ttatcggtct aaagaaagaa atcttggaat tgatctggga agaaagcaaa gcggccgccc
     7501 aggaattcta aatgacaacg ccttggcaac agacgcaata aaaccgtaat gggtttacga
     7561 gccgctcagc cacggccagt aaacccattt ctttatcgaa tgcttgaagg attctttatt
     7621 gtcttatctt ttcagcaatg accccagcaa acctctgacc agttcccggc cgatctgacg
     7681 tcccaccgta ttgacagcgc ttttggtcat ttgttcaaca aacgtatcgg attttcttcc
     7741 cggttttgac cggcttgcag cctggcgctc tttctccgct gccgcttgct cttcctgccg
     7801 ctgctcctct tctttttccc gcagcatttt ggcttgcagc ttttcatagg cagactcccg
     7861 gtcaactgtt ttttcatatt tgccgtacat gattgactgg tttatggcct gacttcttct
     7921 ctgctctgaa atgggtccca tcaaactgcg gggaggcagg ataaaagccc gctgcaccat
     7981 cgcaggactg cctttttcct ccagaaatga gaccagggct tcgccgaccg ccagttccgt
     8041 aatgacatcc gccactttgt agttggggtt ttgacgaaac gtttccgcgg ccgccttgat
     8101 ggccttctga tctttcggcg taaaggcccg cagggcgtgt tgtacccgat tgcccaactg
     8161 ccccagaaca ctgtcgggaa tatcggccgg attttgggta atgaaataga tgcctacgcc
     8221 cttggaacga atcaggcgca ccacttgttc aattctctcc agcaacactt tgggagcgtc
     8281 attaaaaagc aggtgagctt cgtcaaagaa aaagatcatc ttgggtttgt ccagatcgcc
     8341 aacttccggc agttcttcaa acagctcgga aagcatccac aataggaatg tcgaatacag
     8401 cagcggggaa tgaaatagct ggtcacaggc catgatgttg atataaccca tcccatcgcg
     8461 gtctgttttc agcaaatcat aaatatccag ggctggttca ccgaaaaact gatccgcccc
     8521 ctgttcttca atgaccagaa tggcacgttg gatggcaccg atggactgtt tggaaacatt
     8581 tccgtactcc gttgtatatt cggcggcgtt ttccgccaca tagaccagca tggtttttaa
     8641 atctttcaga tcgagaagca acaactgctg gtcatcggca attttaaaag caatatttaa
     8701 aacgccggtt tgggtatcat tcaggcccaa caaacgggat aaaagcaacg gccccatctc
     8761 agagatggtg gtacgtacgg gatgaccttg ccgggcaaac acatcccaga attgtaccgg
     8821 gaaggcatga tagttgaaat tatcgagctc cagtttttta attctttcct gcattttggg
     8881 actatcttcc cccggttcgc acatcccggc aagatctccc ttgatgtcgg taagaaacac
     8941 tggcgtgccg gttgcactaa aggattcagc cagcactttc agcgtaatgg ttttaccggt
     9001 tccggtagcc ccggcaatca agccatggcg gttggccatg gaaggcagaa tatatagttc
     9061 gttttctcct ttggcaatct gcaatccgtt tttcgtcata tcattctcct atatctttat
     9121 tgcactgctg gttatgattg tccgagacga ttttttcttt cttcctccaa ggccttaatc
     9181 aattggtcat agtcttcctg taatttgttg agctcatccg ccagattaag cgctgtcaaa
     9241 acagccacct tgctggtcga gagatggtgg tttctctggc cgatcatgcg catacgccga
     9301 tctacatagg aaccaagcat ttgtatatat tccggatctt cattgccctt aaccacataa
     9361 tcttcgttat aaatactgac cgtcactttg ttgaccgtgt tttttttggc atccacttgg
     9421 cccgccccct tttctaggaa tgagtaatac ttctatactt gcgacgcatt tccttctcat
     9481 cccgaaaaag aaaacctggg cggggttact ccccgtcaat cagcagcccg gtttgatcat
     9541 cgcaataggt tttgcgcatt ctcctcagtc ccggcaacca tgcaggtaat aactttatta
     9601 ttacaacaac aatgttgcac gtgaaacatt gttgttgcag gaatgcctgt tccaaaaaga
     9661 acgtcccggt gtaacatcag gcgcgggcca gtacgttgat gtcaattcgc ttgccttctt
     9721 cattataaac caccatttct gccaggttgg tggcatggtc agcaatgcgt tccaggtggc
     9781 cggcgaccag cagcaaatca gttgcctggg ggatgtttct ggcatcttgc atcatatgca
     9841 gcagcagatc ccgatagacc tgttcataca gttcgtcgga caacttttcc atcccgacca
     9901 aactcatggc catatccaca tcgccgtcga cataggcttt cagggcaatt ctcaccattc
     9961 cttcagcggt ttcggccatg cggggaatat cgatcagcgg cttgatatat ttctgatcat
    10021 gcagacgaat cgtgatttca ccaatatctt cagcgtgatc ggccattctc tccatgtcaa
    10081 cgatgatccg caaagccgta ccgataaaac gcagatcacg ggccatgggt tgtttcaagg
    10141 caatcagctg aaggcattta ttttcaattt ccacagccat ggcatccatg atatcatcac
    10201 gctcaatcac ctcttgggcc atttcaatat ctttgtcaac caacgatttg actgccttaa
    10261 aaatctgttc ttctagaaga cgacccattt tcaaaatttc ttctttcaat ttttttaatt
    10321 cgatgtccag attgtcaacg accattttcc ttaacctcct gtcctttgta tgatcaacca
    10381 aatcttccgg tgatatagtc ttccgtccgt ttgtcaaggg ggttgacgaa gatttcggtg
    10441 gttttgccaa actcgatcag ctcgccgctg agaaaaaatc cggtatagtc agaaacccgc
    10501 gcggcctgtt gcatattgtg ggtcacaatc acaattgtat aatgattctt tagttcccca
    10561 atcaactctt ccaatcgggt cgtggaaatg gggtccaaag ccgaggcggg ttcatccatc
    10621 aggatgactt ccggctccac tgccagcacg cgggcaatta cgagacgctg ctgctgaccg
    10681 cccgacatcc gggctgccgg actcttgagc cgatcactga cttccttcca caagttggct
    10741 gccttcaggc tcttttccac gatttcatcc agccggcttt tgttctttat cccatgaatg
    10801 cggggaccat aggcaacatt gtcataaatg gacatgggaa acaccgatgg tttttggaag
    10861 accatgccga cttttttgcg cagggccacc acatccacat cgctgctgta aatgggctgt
    10921 tgatccagat aaatctgtcc tttgatcgtg gtgccttcga tcaattcgtt taaccggttc
    10981 agactgcgca gaaaagttga cttgccgcag ccggaaggtc caatcagagc ggtcacctta
    11041 ttctcttcta tatccatgtt tatgccctgc agggcttgat attgattgta aaatacctgc
    11101 aaattctgac tttgcatttt gagcgttttt tccatctttc cacctcttct tcagcgcacg
    11161 gcctgcgaaa atttacgcaa actcagattg gccagataat ttatgatcag aatcagaatg
    11221 atgatcaaga aagcggtccc aaaggctcta tccatgg
//
LOCUS       DAOTHZ010000067        10347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_7655_length_10347_cov_8.247862, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000067 DAOTHZ010000000
VERSION     DAOTHZ010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7655_length_10347_cov_8.247862"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="P5549_08080"
     CDS             complement(<1..1132)
                     /locus_tag="P5549_08080"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRW13069.1"
                     /translation="MTASSYNPYENAQAQFDRVAQVIGLDQATCVLLRQPMREFHFTI
                     PVHMDDGSTKIFNGYRIQHNDARGPAKGGIRFHPHETADTVRALAMWMTWKCSVVDIP
                     LGGGKGGVICDPRNMTENEQERLCRGWIRQLAKVVGPVIDVPAPDVMTNGKHMLWMLD
                     EFEAIHGGRYPGFITGKPVGMGGSLGRTEATGYGVIYTVREALKEMGLKIEGSTASIQ
                     GFGNVAQYAAILFQQLGGKVICVSCWDNQDKKAYTYRKADGLDAQALLGIVDSFGTID
                     KQKAQDMGCEILPEDAWIKENVDILIPAALENQINMDNVHSISKQVKIIAEGANGPIN
                     TEADEVVRASGIFVIPDFLANAGGVTCSYFEQVQCNNNFYWEK"
     gene            complement(1381..1548)
                     /locus_tag="P5549_08085"
     CDS             complement(1381..1548)
                     /locus_tag="P5549_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HRW13070.1"
                     /translation="MATDQFEHATFYLTRKQVNEIKELAKQNQISRSALVRMIIREYL
                     AKQDGDRDKKQ"
     gene            complement(1900..3810)
                     /locus_tag="P5549_08090"
     CDS             complement(1900..3810)
                     /locus_tag="P5549_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW13071.1"
                     /translation="MAYDTLKAIPRRINYTAKYQKERAALEKEFQMYLDEWKTYPIMV
                     VNYTKKYGDLPALHHKPYGTWETFSWNQMSEIMFAAASSLLNAGIKEQDRVGIFSANR
                     AEWHLADLGALLMRCVTVPIYATNTEEEAAYIVDDAEIKVLFVGRQVHYDKAYPLLEK
                     CPSLEKIVVFHRDTKIHPDPRVILWDDFLEEGRKTSRKKEIEEIMNRAGYEDMCEIIY
                     TSGTTGEPKGAVHTHKTMMHNAWGVGRYTELGCFVEGDSSMCMLPLTHVLEGSWSFGI
                     LSMGMQLYYCEDHNEILQYLAEANPTAMNAAPRLFEKIYATLFTGIRDASPMKQKIFR
                     WSLAVAKQHGDLVLAGIQPGPLLAMKRKLADKLVLHKVRALFGTGFHHFNYGGAPLNP
                     EIEKFFFYCGVLVTGGYGLTETSPVLAMNGTHCFKFGSVGPTAPLVDIRIDPESGEIQ
                     AKGPNVIKEYYKKPEATREAFTEDGWFRTGDIGRFDEDGYLYITDRLKDLIITSGGKN
                     IAPQLIENLMAEDHFIEYIAVVGDGKKYVSALIVPSYENLEPWAAKEGITFASRAELI
                     KHPQVIAFYEKIITARQKSLGQVERIKKFTLLEHEFSQETGEITPTMKVKRKVVREKY
                     QDLIDAMYADEK"
     assembly_gap    4028..4127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4128..4991
                     /locus_tag="P5549_08095"
     CDS             <4128..4991
                     /locus_tag="P5549_08095"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRW13072.1"
                     /translation="ASSADVNAKAAGTIKTLSFVEGDTVKQGQLLGELSRNDLFAQRE
                     RDAISVLAAEAKLRDLVSGARTQEIEQAQASLKIASRSQAQAEQDLARAESLFAAGAI
                     AQTQVEAARLSAEQKTAQLSQAQAAFNLLQAGNRPQQVAAASAEVNRAKAVLSASEAM
                     LEDLKLYAPQDGVILSKNYEIGEFVALGSALFTIANLNDLWVEVYIPTDDLPKVKLGQ
                     PVDVTVSGDQTVYTGQVTHIANQGEFTPKSIQTKQERTNVVFAVKISLKNPQGILKPG
                     MPADILFHRGE"
     gene            4995..5918
                     /locus_tag="P5549_08100"
     CDS             4995..5918
                     /locus_tag="P5549_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13073.1"
                     /translation="MISVSQASKHFKNLTAVEKVDLQVNKGDIMGLLGPDGAGKTTLL
                     RMICGLIPPDEGEILLLGQPLKQLDRHRDQLGYMPQRFSLYGDLSVMENIYFFGSMYL
                     LKRSAIQQRAEEILTITNLIAFKDRLADQLSGGMKQKLSLTCALITRPSLLILDEPTY
                     GVDPESRKEFWKILYDLNRRGMTLLVSTPYMDEAELCTRVAFISDGKIRLVSSPTDLK
                     NSFSYPVLEIHLQKRHPLRLHDLPGILDVSYMGSKVRVIVRDREQGRNAIEQYLLRQH
                     LSEATIQEVLPAMEDIFVGWAEGGAERWNMQ"
     gene            5903..6826
                     /locus_tag="P5549_08105"
     CDS             5903..6826
                     /locus_tag="P5549_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13074.1"
                     /translation="MEYAISTRNLTRCFGDFVAVDNLTMDIIPGEICGFLGPNGAGKS
                     TAIRMLCGILAPSSGSGTVLGYDLLQETEKIKPKIAYMSQKFSLYDDLTVIENLDFYT
                     GIYSIPARQRRQRMFEMLELAELGGREKALVANLSSGYKQRLALAAAIIAGPELIFLD
                     EPTSGVSPTSRRQFFEIIKQLAEKGTTVIVTTHFMDEAERCDRIAFINQGRLMALDAP
                     DQLKQQFINGCMVSLTVPEPMDHLDKLSSLPYVQEASLHGSHLHVLLAQEEDIALLRQ
                     NCGYVPEQITPSLEDVFIHIARQRKEGGLTS"
     gene            6823..7932
                     /locus_tag="P5549_08110"
     CDS             6823..7932
                     /locus_tag="P5549_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW13075.1"
                     /translation="MNRILAVLKKEMLQIRRDRMTLGIVLMIPLIQLLLFGYAIQTEV
                     KHIPTAVFDQSLSPQSRDMLEAFTASGYFDVTLSVNSYAAVTQSIDSGQAKVGIIFPP
                     DFARSLHRGSPAQVQVLVDASDSMLANQAIATANSIGLIKSQQVLLQKMPNRQLPYDI
                     RVRPWYNPDGITAYYMVPAILGIVVTMTMVILTAVAIVRERERGTLEQLLVTPIKPLE
                     LMIGKILPYIAMGYIQITVALLAGVLVFHVPIRGSLLQLYLLTLFFITASLGLGLMIS
                     NLAKNQMQAFQMSFFIMLPSILLSGFMFPRAAMPRLIYYLSDLIPLTYYLDIIRGIIL
                     KGIGFSYLIGQVTALLVFSIFFLTISTLKFKKKIV"
     gene            7946..8503
                     /locus_tag="P5549_08115"
     CDS             7946..8503
                     /locus_tag="P5549_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRW13076.1"
                     /translation="MSIRDRIIHSCRDLAGSKGFYSLTVDELAAAAGISKRTLYRYFP
                     SKEAIISATLDVFMQEMQRFFQTLLTRNLPDEEALSELFQTLLANGQFIFNPFSLNDL
                     RVHYPFLWERLDRFRSQLIQEFLTYIIAKRERPTLQQINPVLLTAVVQASIQATINPD
                     FLLSNQLGFEEAATQLRNFWIAALL"
     gene            complement(8574..>8963)
                     /locus_tag="P5549_08120"
     CDS             complement(8574..>8963)
                     /locus_tag="P5549_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13077.1"
                     /translation="SEAVRSSSSIPVTFVPKNYCGLKMVDGGVKEIVPASVAKIMGAE
                     YVVSVNLGQEMYDTQVKGIPEIISRTLGILVFETSEMEEEIYSDLTIFPGIKPIPLND
                     TDAIARIIRAGRRAMQENIEEIKRQLR"
     assembly_gap    8964..9063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9064..9248)
                     /locus_tag="P5549_08125"
     CDS             complement(<9064..9248)
                     /locus_tag="P5549_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRW13078.1"
                     /translation="MKSIGLALGGGGLKGMAHIGVLQVLHENGLRPQYITGTSAGSVI
                     ASLYASGISPYQLEAIAL"
     gene            complement(9339..9554)
                     /locus_tag="P5549_08130"
     CDS             complement(9339..9554)
                     /locus_tag="P5549_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13079.1"
                     /translation="MAVRIARADKNEMRARIEEVLVQYKDLHAKIAAYATQTEVEEYQ
                     DFWRQCLQENRRITERLSRYMVIKCNR"
     gene            complement(9621..10253)
                     /locus_tag="P5549_08135"
     CDS             complement(9621..10253)
                     /locus_tag="P5549_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13080.1"
                     /translation="MSDLKCEFPEGCRLDLFAKVVKAKYIARQVKVLEDYVCVPLVYQ
                     EPPLGISVNSCPPPLGHYVSAIDDFKVKVVRAEEILNFNRVRIIIEFDITLFLILDDG
                     RYYCYSLPGNVYEQTIDLCQFDPPLTIEEFRNEVNDSDIILNNWTFDYEIRGCCQDPD
                     SPCFPVMPGVQCPEPILGTCIYLVVYVDIIDKLGKMHDVIVYGELDPEVE"
BASE COUNT         2468 a         2655 c         2266 g         2758 t
ORIGIN      
        1 ctttttccca gtaaaaatta ttattgcact gcacttgctc aaaatagctg caggtcaccc
       61 cgccggcatt ggcaagaaag tcaggaataa caaaaatccc gctggccctg accacttcgt
      121 cagcttccgt attaattgga ccattggccc cttccgcaat gattttaacc tgtttgctga
      181 tgctgtgaac attatccatg ttgatctggt tttccagggc agccgggatt aaaatatcaa
      241 cattttcttt gatccaggca tcttctggca gaatttcgca gcccatatcc tgtgctttct
      301 gtttgtcaat cgtgccgaag gaatccacga tccccaacaa agcctgggca tcaagaccgt
      361 ccgccttgcg ataagtatag gcctttttat cctggttgtc ccagcaggaa acacagatta
      421 ccttgccccc cagctgttgg aacaaaatcg ccgcatattg ggccacattg ccaaagcctt
      481 gaatactggc ggtgctgcct tctatcttca agcccatttc cttgagggct tcccgtactg
      541 tatagattac gccatagcca gttgcttccg ttcttcccaa tgagcctccc atgccaaccg
      601 gtttgccggt aataaaacca ggataacgcc cgccatgaat ggcttcgaat tcatccagca
      661 tccacagcat gtgcttgccg ttggtcatta catccggtgc ggggacatcg ataaccggtc
      721 ccactacttt ggccagttgt ctgatccaac cgcggcacag tctttcttgt tcattttccg
      781 tcatattgcg cggatcacag atgactccac ctttgccgcc gcccagcgga atatccacaa
      841 ccgaacattt ccaggtcatc cacatagcca acgcccgaac cgtatctgcc gtttcatggg
      901 gatgaaaacg gattccgcct ttggccggac cccgggcatc attatgctga atccgataac
      961 cgttgaatat ttttgtgctg ccgtcatcca tatgcaccgg tatggtaaag tggaactcac
     1021 gcatgggctg gcgcagcaga acacaagtcg cctggtcaag gccaatgact tgagctaccc
     1081 gatcaaactg tgcctgcgca ttctcatagg gattgtaaga actggctgtc attatttttc
     1141 ctccttattg tattattaag gtcatcaaaa accattatat ctgattgcat ttgggatttt
     1201 gtataccttt gtgccggtat tctgtataca tatttaactc tattattacg catcgagacg
     1261 ccccattcaa atcggaatga agcacttgta cttttcggtg aaaatacgaa aggtgttttg
     1321 ctcaacttaa gcaaaacacc tttttacgta ttcttttgca ccaccaaaac gaacccctct
     1381 ttattgcttt ttatctctat ctccatcctg tttggccagg tattctctaa ttatcattct
     1441 taccagtgcg ctgcgtgaaa tctggttttg cttggccagt tccttgattt catttacttg
     1501 tttgcgagtc aaataaaaag ttgcatgttc aaattgatct gttgccattt ttaccccctc
     1561 ccccgcccga aactgaaggc tcagcgaaat ctgtgctttg tgcttattat atgttgaata
     1621 aacagcatcg gttacactgc gcatggggtt aaaatcttgt cttggcagag ctgattcccg
     1681 gaaagagtgt aaaagaaggt cagaattttc ctaacctcct cagtcctttt aaaaaacccc
     1741 tcgcttcgtt taacaccaga agcccgttca agagacgtac ttgttacggg aacatccccc
     1801 caaagggtgt gtccccgttc ttcgtgccgc ctccatcacg ggcttcctgc ttacctttga
     1861 ttttgatctt tttgttcacc ccggcagtgt cgctaccgtt tatttttcgt ctgcgtacat
     1921 ggcatcaatc aagtcctggt atttttctct taccaccttg cgttttacct tcatggtcgg
     1981 tgtaatttca cccgtttcct gcgaaaactc atgttccagc agcgtaaact ttttgatgcg
     2041 ctcaacttgc cccaggctct tctgtcttgc agtgatgatt ttttcgtaaa aggcgattac
     2101 ctgtggatgt ttaatcagct ctgcacggct ggcaaaggtg attccctctt ttgccgccca
     2161 tggctctaga ttttcataag acggaacaat caaggcggaa acatattttt tcccatcacc
     2221 caccacagca atatattcaa taaaatgatc ttccgccatc aggttttcaa ttaattgcgg
     2281 agcgatattc tttccgccgg aagttattat aagatccttt aatcggtcgg taatatacag
     2341 atagccgtct tcatcgaatc gaccgatgtc accggttcta aaccagccat cttccgtaaa
     2401 cgcctcgcgg gtggcttccg gttttttata atattccttg atcacattag gccctttggc
     2461 ctgtatctca ccgctttcag gatcaatcct gatatctacc aaaggagcag tcggcccgac
     2521 tgaaccgaac ttgaagcaat gggtaccgtt cattgccagc accggtgacg tttcggtgag
     2581 tccatatccg ccggtaacca gcactccgca atagaagaaa aacttctcga tctctggatt
     2641 gagaggggct ccgccatagt taaaatgatg aaaaccggtg ccaaacagag cccgcacttt
     2701 atgcagcacc aacttgtccg ccagttttct tttcatcgcc agaagcggac cgggctggat
     2761 gcccgccaac accaaatctc cgtgttgttt ggcaaccgcc aatgaccagc ggaatatttt
     2821 ctgcttcatg ggagaagcat ccctgatacc ggtgaagaga gtggcgtaaa ttttttcgaa
     2881 caaacgcggg gcagcgttca tcgccgtcgg attcgcctca gccagatatt gcagtatttc
     2941 gttgtggtcc tcgcaatagt acagctgcat tcccatggac aggattccaa aagaccagga
     3001 accttccaat acatgcgtca agggaagcat gcacatggac gaatctcctt ccacaaagca
     3061 gcccagttca gtataacgcc ccacgcccca tgcattatgc atcatggttt tatgggtatg
     3121 cacagccccc ttgggttcac cggttgttcc ggaagtgtaa ataatttcac acatatcctc
     3181 atatccggcc ctgttcatga tttcttctat ttcttttttt ctggaagttt tcctgccctc
     3241 ttccagaaaa tcatcccata gaataacccg tgggtctggg tggattttcg tgtcgcggtg
     3301 gaaaactacg atcttttcca acgacgggca tttttccagc aggggatagg ctttatcata
     3361 atggacctgc cgtccgacaa agagcacttt gatttctgca tcatcaacga tataagctgc
     3421 ttcttcttct gtattggtag cataaatcgg tacggtgaca catctcatca gcaaggcacc
     3481 caaatcagcc aggtgccact cggcccgatt ggcggaaaag attcccaccc ggtcctgttc
     3541 tttgataccg gcattgagca acgacgaagc tgccgcaaac atgatctcgc tcatctggtt
     3601 ccaggaaaaa gtctcccacg tcccataggg tttatgatgc aacgccggca agtcaccgta
     3661 ctttttcgta taattgacga ccatgatggg ataagttttc cattcatcca aatacatttg
     3721 aaactctttt tccaatgcag cccgttcttt ttgatatttt gccgtgtaat tgatccgtcg
     3781 cggtatcgct tttaacgtat cgtaagccat tttctacctc ctcaacgctt ttataatatt
     3841 aagcatatta tagcacattg gatatttaat attcgaaata tatttctctg tgtgataatt
     3901 cctgcccgtt atcagttttc attgattgaa aacccttaca atcatttttc atccggtgat
     3961 tgccaaagct aatcagagat gctatgctga caccaggtaa actaatttgt tgtcattagt
     4021 atactttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc tcctcggctg
     4141 atgtcaatgc caaagcagcc ggcaccatca aaaccctgtc gtttgtcgag ggtgataccg
     4201 tcaagcaagg tcagctattg ggagagttgt cccgtaatga cctgttcgcc caacgggaac
     4261 gagatgccat cagtgtcctg gcagcggaag ccaaacttcg tgatctggta tccggcgccc
     4321 ggacgcagga aattgagcag gcccaggcca gtctgaagat tgcttcccgc agccaagcgc
     4381 aggctgagca ggacctggcc cgggcggaaa gcttatttgc ggccggtgcc attgcccaga
     4441 cacaagttga agcggcacgg ctgagtgccg aacaaaaaac ggcgcagttg tctcaagccc
     4501 aggctgcgtt taatctttta caggctggca accgtcccca acaagtcgcc gcagccagcg
     4561 ctgaggtaaa tcgggccaaa gcagttttat cagcctctga ggcgatgctg gaagatctga
     4621 agctatatgc accacaggat ggggtcattc tcagcaaaaa ctatgaaata ggtgaatttg
     4681 ttgcgctggg aagtgctttg ttcacgattg ccaatctcaa cgacctctgg gtggaagtct
     4741 atatcccgac ggatgatctc cccaaggtaa aactgggtca gccggtagat gtaacggtaa
     4801 gcggtgacca aaccgtgtac accggtcagg tcacccacat cgccaaccag ggtgaattca
     4861 cgcccaaatc gattcaaacc aagcaagagc gaaccaacgt ggtttttgca gtaaaaatta
     4921 gtttgaaaaa cccgcagggc atattaaaac cgggcatgcc tgccgatatt ttatttcatc
     4981 gcggagagta agccatgatc agcgtaagcc aggcaagcaa gcattttaaa aaccttaccg
     5041 ccgtcgagaa ggtcgatctg caggtcaata aaggtgatat catggggttg ctgggaccgg
     5101 atggagccgg caaaacaacc ctcctgagaa tgatttgcgg cttaatccct cctgatgaag
     5161 gcgagattct gctgctgggc caaccgctta agcagcttga ccgacatcgg gaccaattgg
     5221 gttacatgcc gcagcgcttc agcctttatg gagatctgtc ggtcatggaa aacatctatt
     5281 ttttcggcag catgtacctt ctaaagcgct ctgcaatcca acagcgagcg gaagaaattt
     5341 taaccattac caacctcatc gcatttaaag atcgtctggc cgatcagctc tccggcggga
     5401 tgaaacaaaa gctttccctg acttgcgccc tgatcacccg cccatcattg ctcatcctgg
     5461 atgaacctac ctacggagtc gatcccgaat cacgcaaaga attctggaaa atcctctatg
     5521 atctaaaccg ccggggaatg accttgctag tatccacgcc ttacatggac gaggcggaac
     5581 tgtgtacgcg agtcgccttt atcagtgacg ggaaaattcg tttggtttca tccccgactg
     5641 atttaaaaaa ttcgttttcc tatccggttc tcgagattca tctgcagaaa agacatcccc
     5701 tgcgcctgca tgatttgcct ggcatactgg atgtctccta tatgggttcc aaggtacgtg
     5761 taatcgtgcg cgatcgtgaa caaggccgta atgcaattga acaatacctc cttcgccagc
     5821 atctgtcgga ggcaaccatt caggaagtct tgcctgccat ggaggacatc ttcgtgggat
     5881 gggcggaagg gggagcggaa cgatggaata tgcaataagt acccgcaacc ttacccggtg
     5941 ctttggagac tttgttgctg tagacaatct taccatggat atcatccctg gtgaaatatg
     6001 tggctttctg gggcccaacg gcgccggtaa atcaactgct attcgtatgt tatgcgggat
     6061 tcttgccccc agttccggga gcggaaccgt gcttggctat gacctgctgc aggaaacgga
     6121 aaaaatcaaa ccgaaaatcg cctatatgtc acagaagttc agtctttatg atgaccttac
     6181 cgtgattgaa aaccttgact tttataccgg tatttacagc atacccgctc gtcagcgccg
     6241 gcagcgaatg tttgaaatgc tggaattggc tgagctgggc ggccgtgaaa aggcgctggt
     6301 ggccaacctg agcagcggct acaaacagcg tttggccttg gccgcggcaa ttattgccgg
     6361 gccggaactg atttttctcg acgaaccgac cagcggagtc agtcccacca gccgccggca
     6421 gttttttgaa attattaagc aactggcgga aaagggtact accgtgattg ttaccactca
     6481 ttttatggat gaagccgagc gctgtgaccg aattgccttt atcaatcaag ggcggctgat
     6541 ggcactggat gctcccgacc agttaaaaca gcagtttata aacgggtgca tggtctcatt
     6601 gaccgtgccg gagccgatgg atcacctgga taaattgtcc tcgcttccct atgtgcagga
     6661 ggccagcctg catggttccc atctccatgt tctgctggct caggaagaag acatcgcgct
     6721 gctccgccaa aactgtggat atgtcccgga gcagatcaca ccttccctgg aggatgtatt
     6781 tatccatatt gcccgacaaa gaaaagaagg gggacttaca tcatgaatcg catcctggct
     6841 gtattaaaaa aggaaatgct ccaaatccgc agagatcgaa tgacgctggg gatcgttctg
     6901 atgatcccgc tcattcaatt gctgcttttc ggctatgcga ttcagacaga agttaaacac
     6961 atccccacgg ccgtttttga tcaatctttg tccccccaaa gccgggacat gttagaagct
     7021 ttcacggcct cgggctattt tgatgtcacc ttgtctgtca acagctatgc agcggtcacc
     7081 caaagcatcg acagcggcca ggcaaaagtc ggcatcatct ttcctcctga ttttgcccgc
     7141 agtcttcatc gtggcagccc cgcccaggtg caggtgctgg tggatgccag tgacagtatg
     7201 ctggccaatc aggccattgc cactgctaac tcgattggtt tgattaaatc ccagcaagta
     7261 ttgctgcaaa aaatgcccaa ccggcaactg ccttatgata tccgggtacg gccctggtac
     7321 aatcccgacg gcatcaccgc ttattatatg gtgcctgcca ttctgggcat cgtcgttacc
     7381 atgaccatgg tcattctcac cgcagtggcc atcgttcggg aaagggagcg tggtacgttg
     7441 gaacagttgc tggtcacacc cattaaaccg ttggaattga tgatcggcaa aatcctgccc
     7501 tacattgcca tgggctatat ccagatcacg gtggctctgc tggcaggcgt tttggtcttt
     7561 cacgtgccga ttcgaggcag tctgctgcag ctgtacctgt tgacgctgtt ttttattacg
     7621 gcttcactgg gcctgggtct gatgatctcc aatcttgcca aaaaccaaat gcaggccttt
     7681 cagatgtctt tctttatcat gctgcccagt atcctgctgt cgggctttat gtttccgcgg
     7741 gctgccatgc cgcgtttaat ctactacttg agtgatctca taccccttac ctattatctt
     7801 gatattatcc gtggaatcat tctcaagggg atcggatttt cctatcttat tggacaagtc
     7861 acggcgctgc tggtattttc gatttttttc ctgaccatca gcaccctgaa gttcaagaaa
     7921 aaaattgtgt agcggaggga caattatgtc aatcagggac agaattattc attcctgccg
     7981 ggatctggcc ggcagcaaag gtttttattc cttgaccgtg gatgaactgg cggctgccgc
     8041 cggcatcagt aaacgaacgc tataccgcta tttccccagc aaagaagcca tcatctctgc
     8101 caccctggac gtttttatgc aggaaatgca gcgattcttt cagaccctgc tgacgcgcaa
     8161 tctgcctgat gaagaagccc tgtcggaatt gttccaaacc ctgctggcca atggacagtt
     8221 tattttcaat ccgttcagcc tgaatgatct acgggttcac tatcctttcc tgtgggaacg
     8281 gctggatcgc ttccgttccc agctcattca ggaattttta acgtatatta ttgccaaaag
     8341 agaacggcca acactgcagc aaatcaatcc tgtcctgctg acagctgtgg tgcaggcttc
     8401 cattcaggcc accataaatc cggattttct gctgagcaat cagctcggtt ttgaggaggc
     8461 cgccacgcag ttgcgaaatt tctggatcgc ggcattactg taaagaattc tgccagatcc
     8521 tgccattgga cagcatgctc ccatttttgg ccgtgccggt tggctcgtgc cgtttatctg
     8581 agttgtcttt ttatttcctc gatgttttcc tgcatggcgc ggcgcccggc gcggatgatt
     8641 ctcgcaatgg catcggtatc attgagcgga atcggtttaa tccctggaaa aattgtcaaa
     8701 tcggaataaa tctcttcttc catttccgag gtttcaaaca ccagtatgcc caaggtgcgg
     8761 gatatgatct ccggaatccc cttgacctgg gtatcataca tttcctgccc caaattgacg
     8821 ctcaccacat actcggcacc catgatttta gcgacagaag ccggaacgat ttctttcaca
     8881 ccgccgtcca ccattttgag cccacaataa ttcttgggaa caaaagtaac cggaatagag
     8941 ctgctgcttc gcaccgcctc actnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnagtgcga tggcctccaa ttggtaagga gaaatcccgc tggcatacaa agaggcaatg
     9121 acactgcctg cactggtgcc ggttatatac tgcggcctca gtccattttc atgcagtacc
     9181 tgcaatacgc caatatgagc catgcctttc agtcctccgc cgcccaatgc caaaccgata
     9241 cttttcatct ctttcacccc tgttacttca cgcagagatt tggttactgc agacgttcag
     9301 aaaaatgttt gcaggagcaa ccgctccgat tattatattc agcggttgca tttaatgacc
     9361 atatagcgtg aaagccgctc ggtaatgcgg cgattttcct gcaggcattg ccgccagaaa
     9421 tcctggtatt cttccacttc ggtctgggtt gcatacgctg caattttcgc atgcaaatct
     9481 ttatactgga ccaatacttc ttcaattcgg gcacgcatct cgtttttgtc ggccctggca
     9541 atacgaacag ccattacgta acacctccgt tgctgcaaaa ataaatgccc ggccagtgcc
     9601 gagcatttat ttttgcttgg ttattccact tccgggtcca gttcaccata gacaatgaca
     9661 tcatgcatct tgcctaattt gtcgataata tcaacataga ccaccagata aatacaggtc
     9721 cccagaatcg gttccgggca ttgtactccc ggcatgactg ggaagcaagg gctgtctgga
     9781 tcctggcagc agcctctgat ctcatagtca aaggtccagt tgttcaagat gatgtccgaa
     9841 tcatttactt catttctaaa ctcctcaatc gtcagcggcg gatcaaactg acacagatca
     9901 atggtctgtt catagacgtt gccgggcaat gaatagcagt agtatctgcc gtcgtcaagt
     9961 atcaggaaca gcgtgatatc aaactcgata ataatcctga cccggttaaa attcaaaatc
    10021 tcctcggctc tgaccacctt taccttgaag tcatcaatcg cgcttacgta gtgtcccaat
    10081 gggggcggac aggaatttac actgattcct aacgggggct cctggtaaac caacggtacg
    10141 caaacatagt cctccagcac cttgacctgc ctggcaatat atttggcttt tactactttg
    10201 gcaaacaagt caagcctgca accttcagga aattcacact ttaggtctga catttgactt
    10261 tcccctcctt tttctctaat atttattgac cttatcggtc tgcacagaaa tttgtcaggc
    10321 caaatttcta cgatatatta tgaaaaa
//
LOCUS       DAOTHZ010000068        10318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_7677_length_10318_cov_7.230829, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000068 DAOTHZ010000000
VERSION     DAOTHZ010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_10318_cov_7.230829"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..301)
                     /locus_tag="P5549_08140"
     CDS             complement(92..301)
                     /locus_tag="P5549_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13081.1"
                     /translation="MSAKKSFLLRINPNLFEVYESWAADELRSVNAHLEFILREAAVR
                     AGRWPKGPLPAATIESANKPKRKEQ"
     gene            complement(315..1157)
                     /locus_tag="P5549_08145"
     CDS             complement(315..1157)
                     /locus_tag="P5549_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRW13082.1"
                     /translation="MVEKQAWKMNGFLALLLSLVTLFGAGYAFFQVQILLGILLIVIF
                     ILMVSSIMVVQPNEAKAITFFGSYIGSVRESGLWMTIPFTSKPKVSLRVRNFNSKMLK
                     VNDIDGNPIEIAAVVVFKVIDSAKALFEVNNYEEFVEIQSETALRHVANKYPYDQFEE
                     GGYSLRGNADQVAGELAGEIQERLKSAGVEVIEARLTHLAYATEIASAMLQRQQATAI
                     IAARQKIVEGAVGMVQMALEKLENENIVDLDDERKAVMINNLMVAIVSDRAASPVINT
                     GTLY"
     gene            1691..4132
                     /locus_tag="P5549_08150"
     CDS             1691..4132
                     /locus_tag="P5549_08150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW13083.1"
                     /translation="MKAFEAVKPNGIVLFAGQTIEEACRLLLAEDLYAAPVINSFDQC
                     LGIVSKDILLNALLQDLPADTKIQQVMHSEFGKLAYMDEVPLVNYNVNDFTLVEKDGQ
                     LTGMIDYPYLLMQAALQDDFGRAEFKAATDAVFTPIMAIDNEDRIRILNKWMARVLDL
                     DANQDLLAIATTVIRNPSIIALLKDGENTETRIDLKGNHYISFCKDIIVNRECVGRLL
                     LLRDVSEIEQLVRESEYTKRLNRQLEAIIESSFDGLYLTDGQANTLRLNQGFERIMGI
                     TKEQCIGKNMADLVRKGVFSRSGTLAALETGKTATLSLTSSTGKEAIVTSNPIYDEQG
                     DIIMVVTNVRDITELNMLQRQLERVEDLRQKEMEAVIESMFDGLYVTDGQANTLRINK
                     GFEQVMGITQAQCIGRNMADLVREGVFSRSGTLVALESGKRATISLTASTGKEAIVTS
                     NPIYDDEGNIIMVVTNVRDITELNMLQRQLERVESLQELYKTELEHLKLENTRNLIMT
                     SVKMKELVRMVIRVAAVDSTVLIQGESGTGKELIAEIIHQHSNCRNQPFIKVNCGAIP
                     ENLLESELFGYEGGAFTGASKSGKAGMFELAGEGMIFLDEIGELPLKLQVKLLRVLQD
                     RTIMRVGGIKPIKIDCRVIAATNRDLNTMIDNNQFRLDLFYRLSVIPVMVPPLRERRE
                     DIPVLTNYFLSIFNQKYGMEKRIDTKVYHALEDYKWPGNVRELENLMERLVVTCMSDI
                     IAVNDLPANVRNMPGVLETDAPLIPLKRALENTERDLLKKAFSLYCSSYQIARALGIN
                     QSTVVRKAQKYGIKH"
     gene            complement(4402..5757)
                     /locus_tag="P5549_08155"
     CDS             complement(4402..5757)
                     /locus_tag="P5549_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13084.1"
                     /translation="MDWLKEYERRLITAKEAAQLVKSGDWIEYGFGINCARDFDEALA
                     RRKDELYDVKIRCDIGAYQHFTAEADPTGEHFLWNSWHTAGHDKKFIGKNLYYIPMKF
                     HENPMMTRKDCAPDDVVVLQVTTMDKHGYFNFGCSSVNGWAAMETAKISILEVNEKMP
                     RCLGGNQECVHITQVDYVIESQNQPVMTLGSSEPSEVDVKIAEHIIGRLYDGNCIQLG
                     IGGTPNAVGKMVAASDLKDLGVHTEMYVDAYLDMYKAGKITGSRKKIDRYKQVYSFAM
                     GSQELYDFIDDNPGLASYSVDYTNSPVVIAQIDDFVSINACIEVDLFGQVCSESVGTR
                     HISGTGGQLDFVEGAYKSKGGQSFVCMPSTIEIKGQLASRIKPILSPGAIVTDPRTAT
                     HMIVTEFGIANLKGKSTWERAEALIAIAHPMFQDKLVREAEQMNIWRKSNRLEAAAIS
                     I"
     gene            complement(5818..6984)
                     /locus_tag="P5549_08160"
     CDS             complement(5818..6984)
                     /locus_tag="P5549_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13085.1"
                     /translation="MDFGLTDEQKMFKRTFANFSDEVIQPGAAERDEKEEFDMDCWKK
                     MGELGLAGLIVPEKYEGTAAGAVVYAMAVEEISRNCASLGVTLSAHTSLCTWPILHLA
                     NEEQKQRWLPRLASGEALGALGLTEPGAGSDVGATAMTAVKDGNDFILNGTKIFITNA
                     YYADIYVILVRTGGKDEKHGNSSYFVVEKGTPGFSFGKKEKKLGIRSSSTYELVFEDC
                     RVPQENLIGNVGDGFKQTMHVLNGGRVGIAAQALGIAEGAFRHALAFALERQQFGKPI
                     FANQAISFKLANMAMKIQAARNLVYEVAWREDAGMSYQLQGAFAKCFAGDVAMEVTTE
                     AVQVLGGYGYTRDYPVERMMRDAKITQIYEGTNEIQRLTITNCLKRGEISNYEY"
     gene            complement(7025..8206)
                     /locus_tag="P5549_08165"
     CDS             complement(7025..8206)
                     /locus_tag="P5549_08165"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRW13086.1"
                     /translation="MQREVVLVGACRTAVGTFNGTLQDEPVVNLGAVVMAEALKRAGL
                     KGDQLDEVIFGCVIQAGQGQNVTRQSAIKAGIPHEICAMTINKVCGSGLRAVSLAAQI
                     IQLGDADIILAGGMENMSNAPYGLEKARRGYRMNNGALVDFMIKDGLWEAFNNYHMGI
                     TAENVNEQYGITREEQDAFGFQSQERAAAAIAAGRFKDEIVPVVMKGKKGDIVFDTDE
                     HPRQTTLDKMAALKPAFKKDGSVTAANASGINDGAAAIIVMSREKADELGIKPMAKVV
                     SYASGGVDPKVMGLGPIPASKKALAKAGMTIDDMDLIEANEAFAAQSIAVAREMGWVD
                     KMDKVNVNGGAIALGHPVGVSGARILVSLLYEMQRRQSKYGLATLCIGGGMGTSMIVE
                     M"
     gene            complement(8229..9080)
                     /locus_tag="P5549_08170"
     CDS             complement(8229..9080)
                     /locus_tag="P5549_08170"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRW13087.1"
                     /translation="MAIQKIGVLGAGTMGAGIAQVLVEAGFTVVLVDIKEEFVARGVN
                     GILKNWDKAVDKGKIAAEAAKKYRELLSTAIDNKAFADADIVIEAIIENIDVKKRVLK
                     ELDGICAPGAILATNTSALSITEIAAATSRPDKVVGMHFFNPVPAMKLVEVIPGVETS
                     KATVETVVELSSKIAKEPVPIKESPGFIVNRILIPYINEAAILYQEGVASAAEIDKAM
                     KLGANMPMGPLALADLIGIDVCLMIMEYFLNEFGDSKYRPALSLKQKLRAGHLGRKSG
                     KGFFDYK"
     gene            complement(9103..9885)
                     /locus_tag="P5549_08175"
     CDS             complement(9103..9885)
                     /locus_tag="P5549_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRW13088.1"
                     /translation="MGYENLLLEKEGALALLYVNRPKAMNALNKDTLLEMKEAIISIA
                     SDPGIDALIITGGGNKAFVAGADIAFMKNLTAVEGRAFGALGQEVFRMIEAMEKPVIA
                     AVNGFALGGGCELAMCCDFRIASTNARFGQPEVGLGITPGFGGTQRLPRLIGAGMARQ
                     MLFTADIIDAAEALRVGLVNAVTAPEELMNLVKGIAGRIVKKGQLAVRFTKAAVNEGL
                     QTDIDRAMTIEADLFGLCFSTDDQKEGMTAFMEKRSPQFVGK"
BASE COUNT         2610 a         2730 c         2302 g         2676 t
ORIGIN      
        1 gcactaggga ctttcaccca ttagactgcg cccgtgccgg gcgcaccaaa aaaagttgtt
       61 ccccattttg aggaacaacc tgatgaccct tttattgctc ttttctttta ggtttgttgg
      121 ccgattcaat ggttgcggcc ggcaaaggtc ccttgggcca gcggccggca cggactgcgg
      181 cctcgcgcag gataaattcc aaatgagcat tgacactgcg caactcgtca gccgcccagg
      241 attcatacac ctcaaacagg ttggggttaa tccttaacag gaaactcttc ttggcagaca
      301 tactcccacc acctctaata caaggtgccg gtattgatta caggagaagc tgcacggtcg
      361 gaaacgatgg ctaccatcaa attgttgatc atgaccgctt tgcgttcatc gtccaaatca
      421 acgatatttt cattttccag tttttccaac gccatttgca ccatgcccac ggctccttca
      481 acgatcttct ggcgggccgc aatgatggct gtcgcctgct ggcgctgcag catggcgctg
      541 gcaatttcag tcgcataggc cagatgggtc agacgcgctt cgatgacctc cacccctgcg
      601 gacttcaatc tttcctgaat ctcgccggcc agttccccgg ccacctgatc agcattgccg
      661 cgcagggagt aaccgccttc ttcaaactgg tcatacggat atttgttggc gacgtgacgc
      721 aacgccgtct cgctctgaat ttccacgaat tcttcataat tgttgacttc aaacaaggct
      781 ttggccgaat caataacttt gaaaaccacc actgcagcga tctcaatggg attcccgtcg
      841 atgtcattga ccttcagcat tttgctgtta aaattgcgta cccgcagcga tactttgggt
      901 ttggatgtaa acgggatggt catccacaag ccactttctc tgacagagcc gatataactt
      961 ccaaaaaagg taatggcctt ggcttcgttg ggctgaacta ccataatact ggaaaccatt
     1021 aaaatgaaaa taacgatcag caagatacca agtaaaatct gtacttgaaa aaacgcatag
     1081 cctgcaccaa acaaagtgac cagagagagt aaaagggcca ggaatccatt cattttccaa
     1141 gcttgttttt caaccatgaa aatgcctcct tatatattgt tattatatta atatgatatc
     1201 acttttggca ttttatgcaa ttacaatttt tatcatcttt tttctgtttt cacaatatcg
     1261 cctcccattt tgattcacgt agccaaccaa aatcagttgg ttcccggagt tttttgccac
     1321 gttaactggt tgtttatttt gccaatcact tttgaacggt gccctcttgc ttcgcgcatc
     1381 gttgtcgcct tggacgccgc aaccacagtg acaggatgaa attcgcaaaa atctctgcgc
     1441 aattactgtt tgaattatca taataatatg ataataataa aagtagtaag cgaatagagt
     1501 gcattatcca taccaaaatc tattgcaatg catccctttt tgtcatccca tgcacatgca
     1561 acacttctat caatcaacct tttatttcat gctttatacc gaaacacctg tgattactgg
     1621 catacctgtc cgtgaagcgg atcagggctg agttcataac cttcagccta taaaaggagt
     1681 gtggttgctt gtgaaagcgt ttgaggcagt caagcccaac ggaattgttt tgtttgccgg
     1741 gcaaacaatc gaagaagcct gccgtttgct tttggcagag gatctgtatg ccgcaccggt
     1801 catcaacagt tttgaccagt gcctgggaat cgtcagcaaa gacatcctgc tgaatgcact
     1861 gctgcaggat ctgccggcag atacaaaaat tcagcaagtc atgcacagcg agtttggcaa
     1921 attggcttat atggatgaag ttcccttggt caattacaac gtcaatgact tcacgctggt
     1981 ggaaaaagac ggtcagctga caggaatgat tgattatccc tacctgctga tgcaagctgc
     2041 gttgcaggac gactttggcc gggccgagtt caaagcggcc accgatgcag tctttacgcc
     2101 cattatggca attgacaatg aagaccgcat tcgtatcctc aacaaatgga tggcccgggt
     2161 gttggatttg gacgcgaatc aggatttgct ggccattgcc acgacagtga tccgaaatcc
     2221 gtccatcatt gccttgctga aagatggcga aaatactgaa acaagaattg atttaaaagg
     2281 aaaccattat atctcctttt gtaaagacat tatcgtcaac cgcgaatgtg tcggtcgcct
     2341 gctgttgttg cgtgatgtct cggaaattga acagttggtg cgcgaatccg aatacaccaa
     2401 aagattgaac cggcaattag aggcaattat tgaatcctct tttgacggtt tgtatctgac
     2461 cgatggacag gccaatacac taaggctgaa tcaaggcttt gaacgtatca tgggtattac
     2521 caaggaacaa tgtataggca aaaatatggc tgatctggta cggaaaggtg ttttttcccg
     2581 ctccggaacc ctggcagcgc tggaaaccgg caagaccgca acgctgtccc taacctccag
     2641 taccggcaag gaagccatcg taaccagcaa cccgatttat gatgagcaag gcgacattat
     2701 tatggtggtc accaatgtgc gtgacataac cgaactgaac atgttgcagc gtcaactgga
     2761 aagggtggag gacctgcgcc aaaaagaaat ggaagcagtg attgagtcca tgtttgacgg
     2821 tctgtatgtc accgacgggc aggccaatac actgcggatt aataaaggtt tcgaacaggt
     2881 gatgggtatt acccaggcac agtgcatcgg ccgcaatatg gcggatctgg tgcgggaggg
     2941 tgttttttcc aggtccggga cactggtggc gctggagagc ggcaagcggg cgaccatatc
     3001 gctgacagcc agtaccggca aggaagccat cgtaaccagc aacccgattt atgatgacga
     3061 aggcaacatt attatggtgg tcaccaatgt gcgtgacata accgaactga acatgctgca
     3121 gcgccaactg gaaagagtgg aaagcctgca ggaactctac aaaaccgagt tggaacattt
     3181 aaagctggaa aacacccgca atcttatcat gacctccgtg aagatgaaag aactggtccg
     3241 catggtcatt cgcgtcgcgg cagttgattc gaccgtattg attcagggcg aatcaggcac
     3301 cggcaaagaa ttaattgcgg aaattattca tcagcacagc aattgtcgga accagccctt
     3361 tattaaggta aactgcggag ccatccccga aaaccttttg gaatcagagc tgtttgggta
     3421 tgaaggcggg gccttcaccg gggccagcaa gagcggtaaa gcaggaatgt ttgaactggc
     3481 tggggaaggg atgatctttc ttgatgagat cggggagctg cctttaaaac tgcaagtcaa
     3541 actattgcgg gtgcttcaag atcggacaat tatgcgggtc ggcggcatca agccgataaa
     3601 gattgattgt cgggtcattg ccgccaccaa tcgggattta aacacgatga tcgacaataa
     3661 ccagttccgc cttgatttat tctaccggct cagtgtgatt ccagtgatgg tgcccccctt
     3721 gcgggagcgg cgggaagata ttccggtgct gaccaattac tttctcagca tcttcaatca
     3781 aaagtacggc atggaaaagc gaatcgacac caaggtatat catgccctgg aagactacaa
     3841 atggcccggc aacgtcaggg aattggaaaa cctgatggaa cggctggtgg taacctgtat
     3901 gagcgatatc attgcagtca atgatttgcc tgcgaatgtc agaaatatgc ccggtgtatt
     3961 agaaacagat gcgcccttga tacccttgaa acgagccttg gaaaacaccg agcgggatct
     4021 gctgaagaaa gctttctcct tatactgttc cagttatcaa attgcccggg cactgggaat
     4081 aaatcagtcc acggtagtcc gcaaagcaca aaaatacggt ataaaacatt gatggagaag
     4141 cgccttgcag caagcctcaa ttggcttgac cgcaccagaa agcgccagaa aaaaggaaac
     4201 ccgtgatgga ggccgtacaa gacataagga cactcctcga cgaggaatat ctccataaaa
     4261 tgtacgtgga ttaaacgggc ttcccgcata caacataaat gatggttcac agggtgatgg
     4321 gccgggggga aagcgtggct ttccccctgc cgatcaacgt ccctcttccc ctccccctgg
     4381 ctggaccagt cccttgtttt tttatatgct aatggctgct gcttcaagtc ggttggattt
     4441 tctccagata ttcatctgct cggcttcacg caccagttta tcctgaaaca tgggatgcgc
     4501 aatggcaatc aaggcttccg ctctttccca ggtactcttg cccttcagat tggcgatccc
     4561 gaattcggtc acaatcatat gggtggcagt ccgcggatca gtaacgatcg ctcccggact
     4621 caggattggt ttgatgcggg aagcgagttg tcccttgatt tcaatggtcg acggcataca
     4681 aacgaagctt tgtccacctt tcgatttgta ggcaccttcc acaaaatcaa gctgtccgcc
     4741 ggtgccgctg atgtgacgag tgcccacgct ttctgagcaa acctgaccga acagatcaac
     4801 ttcgatacac gcattgatag aaacaaaatc atcaatttgg gcaatcacca ccgggctgtt
     4861 ggtataatcc accgaatagg aagccaaacc gggattgtca tcgatgaagt catacagttc
     4921 ctgactgccc atggcaaagg aatacacttg tttataacga tcaatctttt tcctggaacc
     4981 ggttattttc ccggccttgt acatatccag ataggcatca acatacatct cggtgtggac
     5041 acccaggtcc ttcagatcag aagcggctac cattttgccc accgcattag gggttccgcc
     5101 gattcccagt tggatacagt tgccgtcata cagccggccg ataatatgtt cggcgatctt
     5161 tacgtctacc tcactaggct ctgaagagcc taaggtcatg actggctgat tttggctctc
     5221 tatcacatag tccacctggg tgatatgaac acattcctga ttgccgccca aacaacgcgg
     5281 cattttttca ttgacttcca ggatgctgat cttggccgtc tccatggcgg cccaaccgtt
     5341 gacggagctg cagccgaaat taaagtagcc atgtttatcc atggtagtga cctgcagcac
     5401 caccacatca tcaggcgcac agtcctttct ggtcatcatg gggttttcat gaaatttcat
     5461 gggaatatag tataagtttt tgccgataaa ctttttgtcg tgaccggctg tatgccaact
     5521 gttccacagg aaatgctccc cggtgggatc agcttcggcc gtaaaatgct ggtaggcccc
     5581 gatgtcacaa cgaatcttca catcatataa ttcatctttt ctgcgcgcca gggcttcatc
     5641 aaaatcccgg gcacagttga tgccgaaacc atattcgatc caatcgcctg atttgaccaa
     5701 ttgggccgct tccttcgccg taatcaacct tctctcatac tctttcagcc aatccaatcg
     5761 cacatcctcc ttcttgaatt ttatttcttt atcgttttcg ggttaacgca atccgattta
     5821 atactcgtaa ttgctgattt ctccgcgttt caaacagttg gtaatggtca gtctctgaat
     5881 ctcattggta ccttcataga tctgggtgat tttcgcatca cgcatcattc tttccaccgg
     5941 ataatcacgg gtgtaaccat agccgcccag cacctgcacc gcttcggtgg taacttccat
     6001 ggccacatca ccggcaaagc atttggcaaa agcgccctgc aactggtagg acatcccggc
     6061 gtcctcccgc caggccactt cgtaaaccag gtttctggcg gcctggatct tcatggccat
     6121 attggccagt ttaaaagaga tggcctgatt ggcaaaaatc ggtttgccaa actgctggcg
     6181 ttccaaagca aaggccagcg cgtggcggaa agcgccttca gcaattccca aagcctgggc
     6241 agcaatacct acccgcccgc cattcaagac gtgcatggtc tgtttgaagc cgtcaccaac
     6301 gttgccgatc aggttttcct ggggaacgcg gcagtcttcg aagaccagtt cataggtgga
     6361 ggaagaacga attcctaatt tcttttcttt tttcccgaag ctgaatccgg gggttccctt
     6421 ttccacaaca aaataactgc tgttgccatg cttttcatct tttccgccgg tgcgtaccag
     6481 gatgacatag atgtcagcat aataggcatt ggtgataaat attttcgttc cattcaggat
     6541 aaaatcattg ccgtctttga ctgcggtcat ggcagtcgca ccgacatccg aaccagcacc
     6601 tggctcggtc aggcccaatg cacccaatgc ttctccggag gccagacggg gcagccacct
     6661 ttgtttttgt tcttcattgg ccaaatgaag gatcggccag gtacacaagg atgtatgggc
     6721 ggacagggtg acgcccaggg aagcacagtt acgcgaaatt tcttctacgg ccatggcgta
     6781 gaccacagcg ccggccgccg tgccttcata tttttccggg acaatcaagc cagccaggcc
     6841 tagttcgccc atctttttcc agcaatccat atcaaactct tctttttcat cccgctcggc
     6901 agctcccggc tgtatgacct catcggaaaa gttggcaaag gttcttttaa acattttttg
     6961 ttcatcggtt aacccaaaat ccatctctca ttacctccta tttttcattc attttgatcg
     7021 acagctacat ttccacgatc atggaagtcc ccatacctcc gccgatacac agggtagcca
     7081 gtccatattt ggactgccgt ctttgcattt catacaaaag gctgaccaga atccgtgcac
     7141 cggagacgcc caccggatgt cccagcgcaa tggcaccacc gttgacgttg actttgtcca
     7201 ttttatccac ccagcccatt tcccgggcaa ccgcgatgga ttgcgccgca aacgcttcgt
     7261 tggcttcgat cagatccatg tcatcaatcg tcataccggc tttagccagg gcttttttgc
     7321 tggcggggat cggtcccaaa cccataacct taggatctac accgccggag gcataactga
     7381 ccactttcgc catcggtttg atgcccaact catcggcttt ttccctggac ataacaataa
     7441 tcgcggcagc accgtcatta atcccggaag cattggcagc cgttacactt ccatctttct
     7501 taaaggccgg cttcaatgca gccattttat ccaaagtcgt ctgacgggga tgttcatccg
     7561 tgtcaaatac aatatcgcct ttcttgcctt tcatcaccac cggcacgatc tcatccttga
     7621 accgtcccgc tgcaatcgcg gcagccgctc tttcctgact ctggaagccg aacgcatcct
     7681 gctcttcgcg ggtaatgcca tattgttcat tgacgttttc tgccgtaatc cccatatgat
     7741 agttattgaa agcttcccac aatccgtcct tgatcataaa atcaaccagg gcgccattgt
     7801 tcatccggta gccgcggcgg gctttttcca acccgtaagg agcattgctc atgttttcca
     7861 tgccgccagc cagaataata tccgcatcac ccaattgaat aatctgggca gccagactga
     7921 ctgctctgag gccagatccg cagactttgt taatcgtcat cgcacatatt tcgtgcggaa
     7981 tccctgcttt tattgccgac tgacgggtta cgttctgtcc ttgccccgcc tgaataacgc
     8041 atccaaagat gacttcatcc aattgatctc ctttaagccc ggctctctta agggcttctg
     8101 ccatgaccac cgcaccaaga ttgaccaccg gttcatcctg caaggtgccg ttgaaagttc
     8161 ctactgcggt gcggcaagcg ccgaccaaca ctacttctct ttgcataatt ttccaaaacc
     8221 tccccgtttt atttataatc aaagaatccc ttgccgctct tgcggcccag gtgaccggct
     8281 cttaatttct gttttaagga aagcgccggc cgatatttgg aatcgccaaa ctcattcagg
     8341 aaatactcca taatcatcag acaaacatcg attccgatca agtccgccaa agccagtggg
     8401 cccatgggca tattggcccc cagtttcatg gccttgtcga tttccgctgc ggaagctact
     8461 ccttcctgat agaggatggc ggcttcattg atataaggaa tcaagatgcg gttgacgatg
     8521 aatcccggtg attccttgat cggcaccggt tccttggcaa tcttgctgct cagttccacg
     8581 accgtttcca cggttgcctt tgaagtctcc actccgggga tgacttccac cagtttcatg
     8641 gccggcaccg gattgaagaa atgcatgccc accactttgt ccgggcggct ggtcgcagcc
     8701 gcaatttctg taatgctgag cgccgaggta ttggtggcca ggattgcccc gggagcacaa
     8761 atcccgtcca gctctttcag tactctcttt ttcacatcga tgttttcgat gatcgcttcg
     8821 atcacaatat cagcatcagc aaaagctttg ttgtcaatcg ctgtagacaa caattcacga
     8881 tatttcttcg ctgcttcagc ggcaatcttc cctttgtcca ctgctttgtc ccagtttttc
     8941 aagattccgt tgaccccgcg ggccacgaat tcttctttga tatcaaccag taccacagtg
     9001 aatcctgcct ctaccagcac ctgggcaatc cccgctccca tagttccggc gcccaaaacg
     9061 ccaatctttt gaatagccaa cctacttcta cctccttttg atctatttac ccacgaattg
     9121 gggagaacgt ttttccatga aagcagtcat gccttctttt tgatcatcgg tgctgaagca
     9181 aagaccaaac aggtctgctt caatcgtcat cgcccgatcg atatcagtct gcagcccttc
     9241 gttgaccgct gccttggtga accgtaccgc cagttgacct tttttaacga tgcgccccgc
     9301 aatacctttg accaggttca ttaattcttc cggggcagtc accgcattga ccagtcccac
     9361 cctaagcgct tccgcagcat caatgatatc ggccgtaaac agcatttgcc tggccatgcc
     9421 ggcaccaatc aaacgaggca gacgttgggt tccgccaaat ccgggggtaa tgcccagtcc
     9481 aacttccggc tggccaaatc tggcattggt tgatgcaatc cgaaagtcac aacacatagc
     9541 cagttcacag cccccaccca gagcgaatcc gttaacagct gcaatgaccg gcttctccat
     9601 ggcttctatc atacgaaata cttcctgccc cagagctcca aacgcccggc cttcaaccgc
     9661 ggtcaggttt ttcataaaag cgatgtcggc acccgccaca aacgctttgt tcccgccccc
     9721 ggtaataatc agggcatcaa ttcccgggtc actggcaata ctgatgatag cctctttcat
     9781 ttccaacaaa gtatctttat tcaaggcatt cattgccttg ggacgattta cataaagaag
     9841 ggccagcgcg ccttcctttt ccagcaataa attttcatac cccatgatat ttcctcccca
     9901 aagacaaatc tcgaattacg catccagcgc gacatgaaaa gcttttatca cctccatcaa
     9961 acaagcatga ataaaacgaa aaaagaaaag ctaaaccagg gaagcgaatc cctgctccgg
    10021 aacacattag tgggatcaat gcagaaatga aaaccgtatc ggaaagtaaa actcgttcac
    10081 cattttttga atattgcaaa tgtcatgcca attccgaaat tttttctttg taaatacacg
    10141 cccgccaaaa tggcaaatcg ctcgtttgcg tagttttttt aaaagggtca taaacgcaaa
    10201 taatgcgatt gcgcattata aaataatgcg caatcgcatt gctgcgaaat tcgttcttgt
    10261 tatttagtgc aaagcaggta atagttgtaa attattttat ctcaatactt tttccaac
//
LOCUS       DAOTHZ010000069        10068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_7973_length_10068_cov_6.706681, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000069 DAOTHZ010000000
VERSION     DAOTHZ010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7973_length_10068_cov_6.706681"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="P5549_08180"
     CDS             complement(<1..280)
                     /locus_tag="P5549_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRW13089.1"
                     /translation="MGRLMINLSFPAMAGMMISAFFALIDTYFVSRLGSTALAALTFC
                     IPIEVLLTSTGSATGVGITSLLARTLGQKKQAEADNIAWHGLIIAVIYG"
     gene            complement(549..1595)
                     /locus_tag="P5549_08185"
     CDS             complement(549..1595)
                     /locus_tag="P5549_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW13090.1"
                     /translation="MRRNVLFIAFWLGLIMLAFPAAALALPDGVPAALEAPQYQKMEL
                     RQNDEGVPYYYIEYSIPQSILDLDQTRPEDNIVGIDVQRKIDNGEWEDYSGGHLDAYT
                     TTSVSGKTNVFSSEIEVLDEGSLEEIQINNHTYSFRLRFWYQYSYGDGPGEWDYVYSA
                     WSNELANQSGSFYKGASAWAIAELDKAQGYGLITERIKDNMSGKITREEFAEVAVKLY
                     ETYTGKIGTVGTASFIDTTNPEILKAANLGLVTGVGNNKYAPNELVTREQMATILLRA
                     LKVINPGADFSNAGAAPFADDAKIKAWARDGVYYCSKASIVNGVGGNMFDPEGNATRE
                     AAVIVCTRAYEYYK"
     gene            complement(1766..4042)
                     /locus_tag="P5549_08190"
     CDS             complement(1766..4042)
                     /locus_tag="P5549_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HRW13091.1"
                     /translation="MTKTWPIAFFNLAAGILLAYYELWIIALCLIMLFILAAVLWKDD
                     SCRIMLCALLLLGGFFWGQLRIADIPVDLPVVQGFQCTGRVLDFPVVDGDKTTFVLKT
                     ERSSRWEKKIRVVCYFQTEVARGDRVKLRGVLKPPRTPGNPGAFNYVRYLAHNGIYYN
                     FSVKNSNSLQIMAPASGPVSWLDGFRVRAERVFQATLPEQEAGILLGMLLGAQAGIDE
                     EQYEAFQKTGIIHLFSVSGLHVGFLLLLVGWIVSLLGLSKGKRFVCGTVVLLLYGTMI
                     AWPVYVIRSIFMGILGLAAYYFGRKNDMMNALALAGVFNLLLDPAALFTISFQLTFLA
                     TWGLIFIFPHLRDVLPWKGWGWDLIWLPLAAELAVMPFIAYYFNILTPVSIITNILVS
                     YLSGVAVVLGFIALFLAPGLPFLATLFLYPAGFFTELILLIVKWMQLVPGGYIWVATP
                     AIFFILLYYAGVITGLYVLGSSWERKWLWPSAAAVLIFLVVLLLPAGFYHRGQLEIDF
                     IDVGQGDAILIKTPQGKFLLLDGGGSMLYDVGDTIVLPYLHRRGIRSLTALLNSHPDH
                     DHLQGLESVAQELTVQTILLPQWLQEAEEYHPLQQIALRKKIDLQMISAGQEINIEEG
                     LRIKALHPGQALSADYNEQSLVMQISYGDFSILLTGDIGKEVMQSLLDKSLITDTTIV
                     KVPHHGSKGSLLPAFYQRLNARYAIISVGPDNQFGHPHPSVLASLEKANLKILRTDQQ
                     GAIIIFTDGRRLQIRPTK"
     gene            complement(4167..6416)
                     /locus_tag="P5549_08195"
     CDS             complement(4167..6416)
                     /locus_tag="P5549_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRW13092.1"
                     /translation="MIGNFFRVTCPYLFKLNTIAYFALIKNRNAYATIARVRETTQLL
                     LDLFRSGDHTYIHPLKVWNRYSPTMFLPHIQQGERFVPITSSTATAQYFSRFQLQGPG
                     NVERKIDYWDRIFLAARELMEENDRQSAEYEQRSATMVDRLCRMVLGRDEYLLQLARQ
                     YFVLEDLLEIRNRLVGSGYIGGKAVGMLLARKILESEDKIKWSDFLEPHDSFYIGSDV
                     YYTYLVENQCWELRQQQKTPENYFALAGPLREKIISGFMPEVIIEQFQQMLDYFGQSP
                     IIVRSSSLLEDGFGNAFAGKYESIFCINQGDPQHRYQQFEKAVKSIYASTMNEDALAY
                     RLQRGMADSDEQMALLVQRVSGSLHDRFFFPDLAGVAMSCNPYPWHEGMDSRAGMVRL
                     VLGLGTRAVDRVDDDYPCLAALDKPTLRPHSDPEEMSRFSQHRVDLLDLRKNEWCSEN
                     LAELFPLLNELNWWKKAASPDYRTMKKLRDWGRTIPDLWTLDFNGLLKGEFPVILRSM
                     LQTLEEAYAYPVDTEFTVNFDVDGRMHINLLQCRPLQTNKSQDSRQYSFAIDQNRLLF
                     STWRNTMGGSVQKKIDRIIFVQPEAYIALSIHDKYRVARLVGRLNRLFDAREEHPYML
                     MGPGRWGSSTPSLGVPVSFAEICHASVLVEVAREEDGLMPELSFGTHFFQDLVETRIF
                     YVALFPGREKIMFHPALLAAYRNEFADWLPEYAIWEEVIKVYDISAAGQELWLDAEIK
                     SQQTHCFFR"
     gene            complement(6580..8418)
                     /gene="asnB"
                     /locus_tag="P5549_08200"
     CDS             complement(6580..8418)
                     /gene="asnB"
                     /locus_tag="P5549_08200"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRW13093.1"
                     /translation="MCGMVGWVDFNNSLRYEEQTIKTMAKTLQHRGPDAEGYWLDRHV
                     ALGHCRLIVVDPEGGRQPMRLATNQGEYILVYNGELYNTEDLRQSLLARGHSFAGWSD
                     TEVLLRAYSEWGENCLPRLNGIFAFAVWDVQNEKLFMARDRIGVKPLFYSQKGSSFLF
                     ASEIKAILAHPGVTAKVDREGLAEIIALGPARTPGHGVFAGISEVKPGYCMMVDRSGT
                     RTKPYWSLISHDHEDSLARTTVKVRELVYDAVKRQLVSDVPLCTLLSGGLDSSAITAI
                     AAEVYKNENNERVQTFSIDYADNEQYFRASELQPDTDAPWIKIVSDYLDTRHASYLAD
                     TPELVQTLYPAVIARDLPGMTDVDSSLWLFSQWIKKQATVGISGECADEVFGGYPWFR
                     QTGDYKLFPWSRHLQTRMKFYSAELLEWIKPGEYVAQRYQEALEEVPRWADDQPTDAR
                     MREMFYLNLTRWMPVLLDRKDRMSMAAGLELRVPFCDHRLVEYVWNVPWEMKNYRSRE
                     KGLLRLALPGLLPEDVLWRKKSPYPKTHNPSYIEAIRNLALAMLADPSSPLLPFINRQ
                     AVRELALTVRSDTDMPWFGQLMNAPQLLAFLLQTDFWLRYYHIEIR"
     gene            complement(8566..9198)
                     /locus_tag="P5549_08205"
     CDS             complement(8566..9198)
                     /locus_tag="P5549_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HRW13094.1"
                     /translation="MGNIDRRYLLAALVLIAVMVFFAGTRYAEMKQNTVMEEDLAVQP
                     STGQEEQANEEELQVYVCGEVEKPGLYKLKAGARVYDALQIAGILPGAALDYAQPARR
                     LQDGETVELYSEAELAEQLAVRQLSPTEALPVVAGIAPASPAGSLVNINTATVQELDD
                     RLPGVGPAIAQRIVDYRESNGGFARIEDLQNVNGIGDKKYSDIKAMITVR"
     gene            9645..9720
                     /locus_tag="P5549_08210"
     tRNA            9645..9720
                     /locus_tag="P5549_08210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9678..9680,aa:Ala,seq:ggc)
     gene            9835..>10068
                     /locus_tag="P5549_08215"
     CDS             9835..>10068
                     /locus_tag="P5549_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRW13095.1"
                     /translation="MDKNYRFDLIEEKWQKHWEENHIFKSYENPDLPKYYTLEMFPYP
                     SGNLHMGHVRNYAIGDVVARFKKMKGFNILHPMG"
BASE COUNT         2623 a         2666 c         2342 g         2437 t
ORIGIN      
        1 ctccgtaaat cacagcgatg atcaatccat gccaggcaat attgtcagcc tcggcttgct
       61 tcttttgtcc cagggtacgg gccaacaggg acgtgatgcc taccccggta gccgaacccg
      121 tggatgttaa caaaacttca atcgggatac agaaggttaa agcggccaat gcagtagatc
      181 ccaaccggga aacaaaatag gtgtcgatca gagcaaaaaa agccgagatc atcatcccgg
      241 ccatggcagg aaaagagagg ttaatcatca gtcggcccac tttatcttcg cccagtgctt
      301 gctttctgat tgccctctcc cccttcttca aaatttatga aaggaaaaaa gagaatagta
      361 ttaattttgc tgtcacagca tgccaaatcc ctttgatcca agcaacctgg tgcagataaa
      421 tgccgcccga gtgagctttg acagcgcaaa acctccgtac agatacgctt gttatctgac
      481 ggaggttttc agcgattaca aagccttatt aattgaaagt aacgaacgca gcgggctccc
      541 ctcagcgttt atttgtaata ctcataagcc ctggtgcaga caataaccgc cgcttcgcgg
      601 gtggcgttgc cttccgggtc aaacatgttg ccgcccacgc cgttgacgat acttgccttg
      661 gaacaatagt aaacgccgtc ccgggcccag gctttgattt tggcatcatc cgcaaacggt
      721 gctgctccgg cgttgctgaa gtcagcgccc gggttgatga ctttcagcgc ccgcagcaga
      781 atggtggcca tctgttcccg ggtgaccagc tcgttggggg catatttatt gttgcccact
      841 ccggtgacca aacccagatt ggcagctttc aatatttctg gattggtcgt atcaataaag
      901 ctggcagtgc cgaccgttcc gatcttgccg gtataagttt catatagctt gacagcaact
      961 tcggcaaatt cttcccgggt gattttgccg gacatattgt ctttgatccg ttcggtaatc
     1021 aagccatatc cctgggcctt gtcaagctcc gcaatcgccc aggcgctggc gcctttataa
     1081 aaactgccgg actggttggc gagttcattg gaccaggcag aatatacata atcccattca
     1141 ccgggaccat ccccatatga atattgatac cagaatctca ggcgaaaact gtaggtatgg
     1201 ttattaatct gaatctcttc cagactgccc tcatccagaa cctcaatttc cgagctgaaa
     1261 acatttgttt tgccggacac gctggtggtg gtataggcat ccaaatgtcc gccggaatag
     1321 tcttcccatt ccccattatc aattttcctt tgaacgtcta tgccgacgat gttgtcctcc
     1381 gggcgggtct gatccagatc cagaatgctc tgggggatac tgtactcaat gtagtaatac
     1441 ggaaccccct catcgttttg cctgagttcc atcttctgat attggggagc ttccagcgct
     1501 gccggtaccc cgtctggcaa tgccagtgcc gcagcaggaa atgccagcat aatcagtccc
     1561 agccaaaaag caataaataa aacatttcgt ctcatcagtt cgacctcctc catcattcac
     1621 cgcaaatgtc attcttaaat tctatagctt aatatataat tcgtaaacca acttggacgt
     1681 tttagggtag cttgtcaaat tttgcgggga tcggaagcaa ggggggacgg aagtaaaaca
     1741 accatccaac cgccctgtgc tgcccctatt tggttggcct gatttgcagg cggcggccat
     1801 cggtgaaaat aataatggcg ccctgctggt cggtgcgcag gattttcagg ttggctttct
     1861 ccagggatgc caggacggat ggatggggat ggccgaactg attgtctggt cctacagaga
     1921 taatggcata acgagcattg aggcgctgat agaacgctgg cagcaaggag cctttgctgc
     1981 catgatgggg gactttcacg atcgtcgtgt cggttataag gcttttgtca agcagcgatt
     2041 gcatgacttc cttgccaata tctccggtta acaggatgga aaagtctccg tagcttatct
     2101 gcatcaccag ggattgctca ttataatccg cagataacgc ctgaccggga tgcagtgctt
     2161 tgattcgcaa accttcttcg atgtttattt cctgtccggc ggaaatcatc tgcagatcaa
     2221 tctttttgcg caacgcgatt tgctgcaaag ggtgatactc ttctgcctct tgcagccatt
     2281 ggggcagcag gatcgtttga accgtcagtt cttgtgccac actttccaaa ccctgtaaat
     2341 gatcgtgatc aggatgactg ttgagcaggg cggtcaaaga gcggatgccg cgccgatgca
     2401 gatacggcag aacaatggta tcaccgacat cgtacagcat gctgccgccg ccatccagca
     2461 acaggaattt cccctgcggc gtcttgatga gaatcgcatc cccctgcccc acatcgataa
     2521 aatcgatttc cagttggccg cgatgataaa aaccggccgg aagcaagaga acgacaagaa
     2581 aaatcagcac agcggcggcg gatggccaga gccatttgcg ctcccaagat gagcccagca
     2641 catataaacc agtaatgact cctgcataat acagaaggat aaaaaatatc gccggcgtcg
     2701 ccacccaaat atagccgccc ggcaccagct gcatccattt gacgatcagg aggatcaatt
     2761 ccgtaaagaa tccggccgga tagaggaaca aggtggccag gaaaggcagg cccggcgcca
     2821 gaaacaaagc gataaaacca agaaccacgg ctaccccgga cagatatgaa accagaatat
     2881 tggtgataat ggagaccggc gtcaggatgt tgaaataata ggcgatgaac ggcatgaccg
     2941 ccaattctgc cgccaacggc agccaaatca aatcccatcc ccagcctttc catggaagga
     3001 catcccgcag gtggggaaag ataaaaatca acccccaggt agccagaaac gtcagttgaa
     3061 aggagattgt aaacaacgct gccggatcaa gcagcaaatt gaatactccg gccagcgcca
     3121 gcgcattcat catgtcgttc ttgcgcccga aataataggc agccaacccc agaattccca
     3181 tgaaaatgga acggatgaca tagaccggcc aggcaatcat ggtaccgtaa agcagcagta
     3241 ccaccgtccc gcagacaaac cttttgcctt tggacagacc caggagggag acgatccatc
     3301 ccaccagcaa gagcaaaaat cctacatgca gaccggatac ggaaaagaga tgaataatgc
     3361 cggttttctg aaaagcttca tattgctctt cgtcaatgcc ggcctgtgcc cccaacagca
     3421 tgcccagtaa aatgcccgcc tcctgttccg gcagggtcgc ctggaaaaca cgctcggccc
     3481 ggacccgaaa cccgtccagc cagctcaccg ggcctgatgc aggggccata atctgtaaac
     3541 tgttgctgtt ctttacgcta aagttgtagt aaatcccatt gtgggcgagg tagcgaacgt
     3601 agttaaacgc cccggggttg ccgggtgtgc ggggtggttt taagactccg cgcagtttta
     3661 cccgatcacc acgggcaacc tccgtctgga aatagcaaac cacccgaatt ttcttttccc
     3721 agcgggagga gcgctcagtt ttcaggacaa aggtggtttt gtcgccatcc acgacgggaa
     3781 agtcgagcac ccggcctgtg cattggaatc cctgtacgac aggaagatca accggaatat
     3841 cggcaatgcg cagctgcccc cagaaaaacc cgcccagcaa cagcaacgca caaagcatga
     3901 tcctgcagga atcgtctttc catagaacgg cggccaggat gaaaagcatt atgaggcaca
     3961 gggcaattat ccataactcg taataagcca gcaagatccc tgccgccaaa ttaaaaaaag
     4021 caatcggcca ggttttggtc acagcgaaaa ttccattctt ttttctacat tatatggaat
     4081 aatctgtatt attaatagtc cctttcgctc aatatgacaa aaagccggcg tgactgccgg
     4141 ctgtacgaaa aacatttttt aaggtctcag cgaaagaagc aatgggtttg ctgactttta
     4201 atctctgcat ccagccataa ctcctgcccg gctgcagaaa tatcatatac cttgatcact
     4261 tcctcccata tggcatactc cggcagccaa tcagcaaatt cattcctgta tgcagcaagt
     4321 aaagcgggat ggaacataat tttttcccgg ccgggaaaca aagccacata aaaaatcctg
     4381 gtctccacca gatcctgaaa aaaatgcgta ccaaacgaca gttccggcat taatccatct
     4441 tcttcccggg ccacttcaac caaaaccgag gcatgacaaa tctccgcaaa agataccggt
     4501 acgcccagag agggtgtact gcttccccag cgtcccggcc ccatcagcat atagggatgc
     4561 tcctcccggg catcaaacag ccggtttaag cgcccgacca gccgcgctac ccgatattta
     4621 tcatggatgg atagggcaat ataggcttca ggttgaacaa atatgatacg gtctattttt
     4681 ttctgcacac tgcctcccat agtattccgc caggtggaga acagcaatcg gttttgatcg
     4741 atggcaaacg aatactggcg actgtcctgt gatttattgg tctgcagcgg gcggcattgc
     4801 agcaaattga tgtgcatcct gccatcgaca tcaaaattaa ccgtaaactc agtatccact
     4861 ggataagcat aagcctcttc caaagtttgc agcatggatc gcagaatcac cggaaattct
     4921 ccctttaata atccgttaaa atcgagcgtc cataaatcag ggatagttct tccccaatcc
     4981 ctaagctttt tcatcgtccg ataatctggt gaagccgcct ttttccacca gttcaactcg
     5041 tttaggaggg ggaatagttc ggccaggttc tcgctgcacc attcattctt acggagatcc
     5101 aaaaggtcga cccggtgctg ggagaaacgg ctcatctcct ccggatcgga atgaggccgc
     5161 agggttggtt tatcgagggc tgccaaacaa ggataatcat catcgacccg gtcaaccgcc
     5221 cgggtcccca agcccagtac gaggcgcacc atgccggccc gggaatccat cccttcgtgc
     5281 cagggataag gattgcagga catggccaca ccggccagat caggaaagaa aaagcggtca
     5341 tgcagggaac cggatactcg ctgtaccagt aaagccattt gctcgtcact gtccgccatc
     5401 ccccgctgca gacgataggc cagggcatct tcattcatgg tgctggcata aattgatttt
     5461 acggcttttt caaattgttg atagcggtgc tggggatcgc cctggttgat acagaatata
     5521 ctttcatact taccggcaaa tgcattgcca aatccatcct ccaggagact gctggaacgg
     5581 acgatgatgg gtgattgacc aaaataatcg agcatttgct gaaattgttc aataatcact
     5641 tcaggcatga atccgctgat tattttttcc cgcaagggcc cggccaaggc aaaataattt
     5701 tccggcgttt tttgctgttg acgcagttcc cagcactgat tttccaccag atacgtataa
     5761 taaacgtccg atccgatata aaatgaatca tgcggttcca gaaaatcaga ccattttatt
     5821 ttatcctctg attccagtat ttttcgcgcc aaaagcattc ccaccgcttt gcccccgatg
     5881 taccctgatc ccaccaggcg gttacggatc tccagcaagt cctccagaac aaaatactgt
     5941 cgtgccaact gcagcagata ctcgtcacgc cctaaaacca tccggcaaag ccggtcgacc
     6001 atggtcgctg atctttgctc atattcagca gattgccgat cgttctcctc catcagttcc
     6061 cgggcggcaa gaaagatcct gtcccaataa tcaattttgc gttccacatt acccggcccc
     6121 tgcagctgaa agcgggaaaa atattgagcc gttgcggtgc tgctggtaat gggaacaaat
     6181 ctttctccct gttgtatatg aggcaggaac atggtaggcg aatagcgatt ccatactttg
     6241 agcggatgga tgtaagtatg atccccgctg cgaaaaagat ccagcaaaag ctgggttgtt
     6301 tcacgtacac gggctatggt tgcataggca tttctgtttt taatcagggc aaaataagca
     6361 atcgtattca gcttaaagag ataaggacaa gtaacccgaa aaaaattgcc aatcatcaga
     6421 tcagtcgccc aagccgaaag cagatcagaa agactgtcaa atatataaca ggctccctca
     6481 ccttcttcgg tagcaatggc gtgcacacgg ctggaaaagg cttcaaatct caggctagcg
     6541 aggagctcca gctggctgct tgtatacttg acgaactgct tacctgatct caatgtgata
     6601 atatctgagc cagaaatcgg tttgcagcaa aaacgccaat aattgcgggg cattcatcaa
     6661 ctgtccaaac caaggcatat cagtatcgct tctgacagtc agcgccagtt cccggactgc
     6721 ctggcggtta ataaatggca gcagaggtga tgagggatct gccaacatgg ccagggccaa
     6781 attgcgaatg gcttctatat aggagggatt gtgggttttt ggataaggac tcttttttct
     6841 ccacagcacg tcttcgggaa gcaaacctgg caaagccagc ctcaacaatc ccttttcccg
     6901 gctacggtag ttcttcatct cccagggtac gttccataca tattccacca accggtgatc
     6961 acaaaaaggc acccgcagtt ccaatcccgc cgccatgctc atccggtctt tgcgatccag
     7021 cagaaccggc atccagcggg tcagattcag ataaaacatc tcccgcatgc gggcgtctgt
     7081 aggttgatcg tctgcccaac ggggcacttc ttcaagcgct tcctgatatc tttgggctac
     7141 atattcccca ggtttgatcc attccagcaa ttctgccgaa taaaacttca tgcgggtctg
     7201 caaatgccgg gaccatggaa acaatttgta atcgcctgtt tgacgaaacc agggatatcc
     7261 accaaaaacc tcatccgcgc attcacctga tattcctact gtagcctgct ttttgatcca
     7321 ttgactgaac agccacaatg aagaatcgac gtccgtcatg ccgggtaaat cccgcgctat
     7381 gaccgccggg taaagggtct gtaccaattc aggcgtatcc gccagatagg atgcgtgtct
     7441 ggtatccaaa taatccgaga cgatctttat ccagggagca tccgtgtcgg gctgtagttc
     7501 actggctcga aaatactgct cattgtcggc ataatcaatg gaaaaggttt gcacccgctc
     7561 gttgttttca tttttatata cttcggcggc aatcgcggta atcgcgctgg agtcaagacc
     7621 gcctgataac aaagtgcata aaggcacatc tgataccaac tgacgtttaa ccgcgtcata
     7681 aaccagttcc cgtaccttaa ccgtcgtccg ggccaggctg tcttcatggt catgactgat
     7741 cagggaccaa tatggttttg ttcttgtccc gctgcggtca accatcatac aatagccggg
     7801 ctttacttca gatatacctg caaacactcc gtgtcccgga gtgcgggccg ggcccagggc
     7861 aataatctcc gccaggccct cccggtcaac tttggcggtc acgccggggt gagccagaat
     7921 ggctttgatc tcggaagcaa aaaggaatga actgcctttc tgagaataaa acaagggttt
     7981 gacaccaatc cgatctctgg ccataaacaa tttctcgttc tgtacatccc acacggcaaa
     8041 ggcaaaaatg ccgttcagcc tgggcaggca attttcaccc cattcactat aagctctcag
     8101 cagtacttcc gtatccgacc aaccggcaaa ggaatggccc ctggccagca agctttggcg
     8161 caaatcctcg gtattgtaca actccccgtt gtaaaccaga atatactctc cttgattggt
     8221 tgccagtctc atcggctgcc gtccgccctc cggatcaacc acaatcaaac ggcaatgtcc
     8281 cagggcaacg tgccggtcaa gccaataacc ctccgcatcc ggaccgcgat gttgcaacgt
     8341 ttttgccatg gttttgatgg tttgttcttc ataccgcaat gaattattaa aatccaccca
     8401 gccaaccata ccgcacataa gccatgcacc tccatcaaaa aaagcgttcc gggcaaagct
     8461 caacaagctt tgctcagaac gccgttgttc tgttcaagga taatatatgc agaatttata
     8521 aaggatgtgc ctgtgaccta cccgttgcag tgacaaatct aattgctagc gcacggtaat
     8581 catagctttg atatccgaat attttttatc cccaatgccg ttgacatttt ggagatcctc
     8641 gattcgggca aatcccccgt tgctctcccg ataatcaacg attcgctggg caatggccgg
     8701 accgacgccc ggcaaacgat cgtccagttc ctgtaccgtt gcggtattga tattgaccag
     8761 actgccagcc ggagatgctg gggctatccc tgccacaacc ggcagcgctt ccgttggtga
     8821 aagctgccgg acggcaagct gttcggccaa ctccgcttcg ctgtaaagtt ccaccgtctc
     8881 cccatcctgc agcctgcggg ccggctgggc ataatcaagc gccgcaccgg gaaggatgcc
     8941 ggctatttgc aaagcatcat agacccgggc accggctttg agtttataca gaccgggttt
     9001 ttccacctca ccgcagacat agacctgcaa ttcctcttcg ttggcttgtt cctcctgccc
     9061 cgtgcttggc tgcaccgcca ggtcttcttc catgaccgtg ttttgtttca tttccgcata
     9121 tctggtcccg gcgaaaaaga ccatcacagc gattaaaacc agcgccgcca gcagatagcg
     9181 acgatcaatg tttcccatat cgccacctcc tgtatacttt ttctttgcat tatatccatt
     9241 tccttcttta attgccattt aaattattta atttacattt atctccttgt ctcgatggtt
     9301 ctgtttccat cccatccccg tatgacatcc aaaatgtccg gtgaacagcg taaagcaatc
     9361 tttatctccc gttttgattg catttacttt gtctattgac gtgatttaaa aataagcctg
     9421 atgaatcgtt taatcggttg actttgcgga taatctattt tataattgca aatgtcaatt
     9481 gaacattgaa tcgatgatcc ggagtagtag gcagatacca agcagagagc tgccgtttgg
     9541 tgaaaggcag caatcctgac gaactcgccg gggagaattg caggtgaatg attttttagc
     9601 ctgcgacggg acatccgtta acggttcaag tggacaattt ttgtggggct atggcgaagt
     9661 tgggatcgcg cctgaatggc attcaggagg ccgtgggttc gagtcccact agctccacca
     9721 tatttaaatt ttgtcaatca gggtggtacc acgggaaaac agctctcgtc cctttcgggg
     9781 aggagagttt tttgtatata caattttttt gctcagagcg gcaaggagga aatgatggac
     9841 aagaattacc ggtttgattt aattgaggag aaatggcaaa agcactggga agaaaatcat
     9901 attttcaagt cttacgaaaa ccctgatttg cccaaatact atacactgga gatgttcccc
     9961 tacccgtccg gcaatctgca tatgggtcat gtgcgcaatt atgccattgg agacgtcgtc
    10021 gcccgtttta aaaagatgaa aggatttaat attcttcatc ccatgggc
//
LOCUS       DAOTHZ010000070         9692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_8353_length_9692_cov_6.275397, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000070 DAOTHZ010000000
VERSION     DAOTHZ010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8353_length_9692_cov_6.275397"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="P5549_08220"
     CDS             complement(<1..384)
                     /locus_tag="P5549_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW13096.1"
                     /translation="MIQIKNVYKSFGHLEVLKGINCYIAPREVVCVIGPSGSGKSTLL
                     RCINQLETVQSGDIIIDGQDITHPSIDINLIRQQVGMVFQLFNLFPHKTTLENITLAP
                     TKILKISTDEAHERAMTLLRKVGLVD"
     gene            complement(381..1091)
                     /locus_tag="P5549_08225"
     CDS             complement(381..1091)
                     /locus_tag="P5549_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRW13097.1"
                     /translation="MIQVIPFTFAFNPFQALLDIHGFEVVAHNFPSFMVGALITLELT
                     ALAVAFGMILGLLFGLLKMAPQFKPTYWLATLYIDFFRGTPLFVQILLLYFGILPVFG
                     EIGAFTSAVIACSLNSGAYIAEIVRAGIQAIDKGQMEAGRSLGMNYRQAMWYIILPQA
                     YKVVIPPLINEFIAMLKDTSLVSIISVSELTLRGKLIYASSYEPAWVWGTVAIIYFIM
                     TKLLAMFGDYMERRLATE"
     gene            complement(1174..1956)
                     /locus_tag="P5549_08230"
     CDS             complement(1174..1956)
                     /locus_tag="P5549_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRW13098.1"
                     /translation="MKKLTAILISALLLVGILALSGCGSKDAGEQAAPEKVLRVVTEA
                     GFAPFEFKEGNDEFTGFDMDLIRAIGEAQGYKVELAHMGFESLIPALQSDKADCAIAG
                     MSITPERQESVDFSTPYFDAGLIIAVAKDTEGIATLDDLQGKKIGCQVGTIGADASMS
                     VKEKDSKTEVRTFDNIGEAFMELEKGGVDAVVNDHAVTAYYIKTTGKDKTKMVGELFS
                     ADDHYGIAVKKGNAEMLKVINEGLGKIKANGKYDEIYKKWFE"
     gene            2240..3112
                     /locus_tag="P5549_08235"
     CDS             2240..3112
                     /locus_tag="P5549_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRW13099.1"
                     /translation="MITKHKVKRNEAEFLMMLVALIWGVTFVLVKNALADIGPYLFLG
                     IRFTLAFLVLALFSWRNVLKIEVSTVAAGCLLGFFLFIGYVFQTVGLQYTTSSNAGFI
                     TGVSVVLVPIMFYLKNRLWPDWQTTLTVIMAATGLLLLSVPAGKFVLGYGDLLVLVCA
                     FGFALHIVFVSKYSWRHNAVAITGVQILFVGILCLIIGLATEPIPQYLGTDTILAIIV
                     TSVFATSLAFLLQNHLQQFSTPTRFAVVLTTEPVFAALAGFVWANEILTVRAYSGAAL
                     ILIAMLLSILLRKN"
     gene            3249..4097
                     /locus_tag="P5549_08240"
     CDS             3249..4097
                     /locus_tag="P5549_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRW13100.1"
                     /translation="MRKFVSLALCAMLVVGLLVSVTGCGSTDKDQAAAPSNEFKVGME
                     CGYPPFNWTQSDNSNGAVPIAGSKEFAGGYDVEIAKLLAEGMGKELVIVKTAWDGLSP
                     AVQAGTIDAIIAGMSPTAERKQTIDFSDHYYRSDLVVVIQKGSKYESATSLQELAGAK
                     ITAQLNTFHYTVIEQIPDVQKQTAMDDFPAMRVALESGIIDGYVSERPEGVSATTANP
                     KFAMVEFAAGKGFQASDDDVAIAVGLKKGNPELVSSINKVLSGVSEEQRKAIMDQAIK
                     NQPASN"
     gene            4129..4920
                     /locus_tag="P5549_08245"
     CDS             4129..4920
                     /locus_tag="P5549_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRW13101.1"
                     /translation="MSVTADSTFFQWVAFIFQEYWPLFLRGAEVTLIISLTGTIIGFC
                     IGILIGVIRTIPIDQNTPLFKRILFKIINFILLCYIEIFRGTPMIVQAMVIFYGVAMV
                     YNIHLSTFFAGIFIVSINTGAYMAEIVRGGILSIDKGQFEAAHSIGMTHAQTMLHIIL
                     PQAVRNILPATSNEFVINIKDTSVLNVIGVTELFFMSKSAAGNNFRYFEVFLITCSVY
                     FVMTFTVTRILRFFEMKLEGPSVYTIHGSQTVPEAEFKIVDRGKH"
     gene            4920..5657
                     /locus_tag="P5549_08250"
     CDS             4920..5657
                     /locus_tag="P5549_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRW13102.1"
                     /translation="MENIIEIRHLQKSFGQNEVLKDINFSVNKGEVVCIIGASGSGKS
                     TLLRCINLLELPTNGEILYHGQNILQHNMTTAVYRAKMGMVFQQFNLFNNLNVLENCV
                     VGQVKVLKRSKAEARKTALEYLDLVGMSTFINAKPNQISGGQKQRVAIARALSMEPEA
                     LLFDEPTSALDPEMVGEVLKVMKNLAKSGLTMLVVTHEMAFARDVAHRVVFMDDGVIV
                     EEDTPDVIFNNPSNERTRIFLKRILDN"
     gene            5945..6937
                     /locus_tag="P5549_08255"
     CDS             5945..6937
                     /locus_tag="P5549_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW13103.1"
                     /translation="MDTAKMWEEGWLKKQGDIYILRMFMPFPLKENNCFIIEGEDGWV
                     VLDTGVSLEQNQDLLNSALRSIGITLRRLKAVYLTHYHHDHSGLAGWLQAKADIPVYL
                     PQEDLVTWQRFIDTDQYFDSAITECQQEGWPEEYVHELSDNIRFINTYLQPLPEFIPY
                     SDGLTVKLHGDDYLTIAVPGHTDGHMVYYNPARGILFPGDNVVDHTILHLTDWPHTYL
                     LNPCDLHLQALTGLKKLPVELVIPGHGRTFANLNDKIDLIVAHHEKRKRIVYEAVKTP
                     STAWEIAADVFKPNQYIHIRRLVLAETLGYLASLLNEGRVERELSSGQYIYRRS"
     gene            7129..>9692
                     /locus_tag="P5549_08260"
     CDS             7129..>9692
                     /locus_tag="P5549_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRW13104.1"
                     /translation="MQRKVMTGHKSGLFILLALFFLISCLIMPPPATCQAQDTADTTL
                     TGRITAANTGAGIGAVSVRLSSLANETLYEVKTDDFGFYRMDGLEAGSYIVTAEHPAY
                     QTAQEINFAVNPGANCWDWALQPISGGASLFDVYVEVVCATSGIHLADVPVAITVKRD
                     SDNQFVTYNQATDADGVAHLTGMPAGVYTFTINYDIPNVAAKIGGWDGYISVPFEDIS
                     GPHWADVRLKPIYNQLNVTVKGYDVVKDQADMPLGGVIVEASGCDPADDSNVLLPPQV
                     GVSGLTKDAPYWDNSMAGKVKFDKLIPLTWLLETKKLGYENKNLFVRSNSSGGLMQTD
                     VTINIPLEDTKLWVWLDSPYNDPEMLEGLDVILEGITGQNTEGIARRVKAVYSTEEDS
                     TYALFNNILPGNYDVRVDAKIDKTVPIEVDGKKIYDGLYGAPSDKQFTVRFTAQETFY
                     AENDKNTDVTLQLTPEKMKLSGVLCTVDGERATDSAYAQYAGAPAAGKNVEIRLSEYM
                     NTAFPAEEKLHQTVTDASGNFSVELLPGLYGMVVTGMDDYWGNSADLSDAAHPDQWLY
                     TYHAWPYYQMWPYSYESALAFKDRSPQNNEPMSDIGGMPVSSGQTLTGTFVVRKDVFN
                     YDVDIKRGLQDPFEHMVVGIDRSGSSSKVITHTYDMIKNGGQLTLNGPDSQIQAVDLG
                     TTEPSFKNLPAGQYSLTLTHPRYTQIWTPTTTFFDFPSPGMLPSVAFPENYNSFQSPG
                     PGPWPLLPWSAFFRPEYAMTGAIQVSNMMTEEHRVRVMKWTQISSDPVEWDYVEVTGQ
                     AYPRFIKTAYTGDLLFETERTMNDPTALATTAYVPNTNYDAYFWWPAGNSLFDDAA"
BASE COUNT         2349 a         2424 c         2437 g         2482 t
ORIGIN      
        1 gtccaccagt cccaccttgc gtaacagagt catggctctt tcatgggcct catccgtact
       61 tatctttaag attttggtcg gtgccagggt gatattttcc aacgtcgtct tgtggggaaa
      121 gagattaaat aattggaata ccatgcccac ctgctgacgg atcagattga tgtcaatact
      181 gggatgggtg atgtcctgac catcaataat gatatctcca gattggaccg tttccagctg
      241 gttgatacag cgcagcaagg tcgacttgcc cgaaccgctg ggcccgatca cacagactac
      301 ttcccggggg gcgatataac agttgatccc tttcagcact tccaaatggc caaaactttt
      361 gtagacattt ttaatctgga tcattcggta gccagcctcc tttccatgta atcaccaaac
      421 atggccagga gtttggtcat gataaagtaa ataatggcaa ccgttcccca cacccaggcc
      481 ggttcataac tgctggcata gatcagtttg ccgcgcaggg tcagttcgga aactgagatg
      541 attgatacca gggaagtatc cttgagcatg gcaatgaact cgttgatcag cggaggaata
      601 acaactttgt aggcctgtgg caaaatgatg taccacatgg cctggcggta attcatgccc
      661 aaggagcgtc ctgcttccat ctggcctttg tcgatggcct gaatgccagc gcggacgatc
      721 tctgcgatat aggcaccgct gttcaggctg caggcaataa cggcgctggt aaaggctcca
      781 atctcgccaa agaccggcag gatgccaaaa tagagcagca aaatctgcac aaacagcggc
      841 gtgccccgaa agaaatcaat atacagggta gccagccagt aggtcggttt gaattgtggt
      901 gccattttga gcaaaccaaa cagcaagccc aggatcatcc cgaaggctac cgccagggca
      961 gtcaactcca aagtaatcag tgcgccgacc atgaatgaag gaaagttatg ggccactacc
     1021 tcaaacccat ggatgtccag caaggcttga aaaggattga aagcaaaggt aaagggaata
     1081 acttgtatca tatgaacctc ccggaacgat cattaagcaa gaaaagcgcg aggattgtcc
     1141 cgcgcttttc tcttctgcgc ttaccgcttg atcttattca aaccacttct tgtagatttc
     1201 gtcatatttg ccgttggctt tgatttttcc cagtccttca ttgatgactt tgagcatttc
     1261 agcattgcct ttcttgacag caatgccata atgatcatca gcagagaaca gttcgcccac
     1321 catcttggtt ttgtccttgc cggtggtctt gatataataa gcagttaccg catggtcatt
     1381 gacaaccgcg tcaacgccgc ctttttccag ttccataaag gcttcaccga tattgtcaaa
     1441 cgttctgacc tcggtcttgg aatctttttc cttgacgctc atgctggcat ccgcaccaat
     1501 ggtgcccacc tggcagccaa tctttttgcc ttgcagatcg tccagcgtcg caatgccttc
     1561 agtatccttg gcgacagcga taatgagacc ggcatcaaaa taaggggtgc tgaaatcgac
     1621 tgattcctgt ctttccggtg taatcgacat accggcgatg gcacagtcag ccttgtcaga
     1681 ctgcaaggcc ggaatcaggg attcaaatcc catgtgggcc agctctacct tgtaaccttg
     1741 cgcctcacca atggctctga tcagatccat gtcaaaacca gtgaactcgt cgttgccctc
     1801 tttaaattca aagggagcaa aacctgcttc ggttacgacg cggagaactt tttccggtgc
     1861 tgcctgctct ccggcgtcct tgctgccaca gccgctcagg gccagtatgc ccaccaacaa
     1921 gagggcggaa ataagaattg cggttagttt tttcaacgat gtacacatcc tttctcttct
     1981 atactaattt cctaataatt atacagctag actaatattc cgacaagtaa tttccggtca
     2041 gttgtttttt atcgggctgt ttgttaaaaa cttttcgttt ctggtttgca ccgcttgcgc
     2101 tgcaaaaagc agcgactttt ttattgtttg catttgcata caaatgtaag cttcaattgt
     2161 gttatgatat tcggggagtt ggcaatgcta ttcatagctg gactgacgcg gaattagtgc
     2221 gaatccggag agtaaaacgg tgataacgaa acataaagta aaacgcaatg aagcagaatt
     2281 tttaatgatg ctggtggcac tcatctgggg cgttaccttt gtactggtta aaaatgccct
     2341 ggccgacatc ggtccctatc tatttctggg gattcgattc actttggcat ttctggtgct
     2401 ggctctattt tcctggagaa acgtgttgaa aatcgaagtt tcaaccgtgg ctgccggttg
     2461 tctgttggga tttttcctgt ttatcggtta tgtatttcaa acggtgggac tgcaatatac
     2521 gacttcttcc aatgccggct ttattaccgg ggtttcggtg gtgttggtgc ccatcatgtt
     2581 ttaccttaaa aaccgtctct ggccggactg gcaaaccacc cttaccgtaa ttatggcggc
     2641 gacagggttg ttattgctgt cggtaccggc gggcaaattc gtgttgggct acggggattt
     2701 gctggtgctg gtctgcgcct ttgggtttgc cctgcatatc gtgtttgttt ctaaatattc
     2761 ctggcgtcac aatgcggtgg ctattaccgg cgtacagatc ttgtttgtgg gaatcctgtg
     2821 cctgatcatt ggtctggcaa cggaacctat ccctcaatat ctgggtacgg ataccatcct
     2881 ggcaattatc gttacctcgg tatttgctac ttcgctggcc tttttgctgc agaaccatct
     2941 gcagcagttc agtactccaa cgcgctttgc tgtggtcctg actacggagc cggttttcgc
     3001 agccctggcc ggctttgttt gggccaatga gattttaacc gtccgggctt acagcggagc
     3061 agccttgatt ctgatagcca tgctgctttc aattttattg cgaaaaaact aatcattata
     3121 cagattcccg ggaattcagc tcagatattg ctgataaaga ggttccttca ttgcattttt
     3181 atgttacaat tatttcgatt ggtatggacg gtaacaccga aaattttatt tgtgaggaga
     3241 agtggttaat gaggaagttt gtaagtttgg cgctttgtgc aatgctggtt gtggggctgc
     3301 tcgtctcggt tacgggttgt ggttccactg acaaagatca ggctgcggcc cccagtaacg
     3361 aattcaaagt gggcatggaa tgcggttatc cgcccttcaa ctggacccaa tctgacaatt
     3421 ccaatggcgc ggtccccatt gccggcagca aagaatttgc tggcggatat gatgtagaaa
     3481 ttgccaagct tttagccgaa ggcatgggca aggaactggt tatcgtgaaa actgcctggg
     3541 atggactgtc tccggccgtc caagccggta cgattgacgc catcattgcc ggtatgtccc
     3601 ccactgcaga aagaaaacag accatcgatt tttctgatca ttactatcgt tccgatttgg
     3661 tggtcgtgat tcagaagggt tccaaatatg aaagcgccac cagtttgcag gagcttgccg
     3721 gagccaagat tacggcccag ctgaacacat tccattacac tgtaatcgaa cagatcccgg
     3781 atgtgcaaaa gcaaaccgcc atggatgatt ttccggccat gcgtgtagca ctggaatccg
     3841 gcatcattga cggttatgtt tccgaacggc cggaaggcgt ttccgccact accgccaatc
     3901 ccaagtttgc catggtggaa tttgctgccg gcaagggatt ccaggcttcc gatgacgatg
     3961 ttgccatcgc ggtcggactg aaaaaaggca atccggaact ggtcagcagc atcaacaagg
     4021 ttctgtccgg cgtctctgaa gagcaaagaa aagccattat ggatcaggct atcaagaatc
     4081 aacctgcttc gaactaatat tcgcagctaa caggagttgt ttttattcat gtcggttact
     4141 gcagacagta ctttttttca atgggtagca tttatctttc aggagtactg gccccttttc
     4201 ttaagagggg ccgaagtaac cctgattatt tctttgaccg gtaccattat tggtttctgt
     4261 atcggcattt taattggtgt tatccgtacc atcccaatcg atcagaatac ccctctgttc
     4321 aaacgtatcc tatttaaaat tattaacttt atcttgctgt gttacattga aatctttcgc
     4381 ggcacgccca tgatcgtaca agccatggtt attttttatg gggtggcaat ggtttacaat
     4441 atccatctat ccaccttctt tgccggtatc tttatcgtct ccatcaatac cggcgcgtac
     4501 atggcagaaa tcgttcgagg cggcatccta tccatcgaca aaggacagtt tgaagcggct
     4561 cattccatcg gcatgactca tgcgcaaacc atgctgcata tcatcctgcc ccaggcagtg
     4621 cgcaatattc tgccggccac cagcaacgaa tttgtcatca atatcaagga cacctcggtg
     4681 ctgaatgtca ttggcgttac cgagctcttc tttatgtcca aatctgccgc cggcaacaat
     4741 ttccgttatt tcgaggtatt cctcatcacc tgctcggtat attttgtgat gacctttacc
     4801 gtaacccgta ttctgcgttt ctttgaaatg aaactggaag gtccttccgt ctataccatt
     4861 cacggttccc agactgttcc tgaagcagaa tttaaaatcg tggacagggg gaaacattga
     4921 tggaaaatat cattgaaatc cgtcatctgc aaaaatcttt cggacaaaac gaagtgctca
     4981 aggacatcaa cttttccgtc aacaagggag aagtggtctg tatcattggg gccagcggtt
     5041 ccggcaaatc taccctgctg cgctgcatta acctgttgga attgcccacc aacggagaga
     5101 tcctctacca tggacagaac attctccagc acaatatgac cacggccgtc tatcgcgcca
     5161 aaatgggcat ggtttttcag cagtttaatc tcttcaacaa cctgaatgtg ctggaaaact
     5221 gtgttgtagg tcaggtcaag gtactgaagc gcagcaaagc cgaagcccgc aaaacagctt
     5281 tggaatatct ggatctggtg ggcatgtcca cttttatcaa tgccaagccc aatcagattt
     5341 ccggcggtca aaaacagcgc gtggcgattg cccgggccct ttccatggaa ccggaggccc
     5401 tgctgttcga cgaaccgacc tcggcgctgg atccggaaat ggtcggtgaa gtcttgaagg
     5461 ttatgaaaaa cctggccaag agtggactta ccatgctggt agttacgcat gaaatggcct
     5521 ttgcccgtga tgtagcccac cgggttgtgt ttatggatga cggtgtaatc gtggaagaag
     5581 ataccccgga cgttatcttt aacaacccca gcaatgagcg aacccgtatc ttcctgaaac
     5641 gcattctgga taattaagtt gatttcttgc cgtcattgcc gacgattcat tttaaagtac
     5701 cgataaactg gctgccatcg ctccaggcga aaccaaataa ccgcaagttc tacaggaatc
     5761 ccgtgatgga agcgtgccca tacgacgctg taccaacggg attttactgt ttgacggcac
     5821 tccctggtgc agcgctttat cgtctgcgca ccacccggaa gatttggttt tgacaaattt
     5881 ctgcttgcct gccgggatgg tttggcaaat aataggagta tatctgctga cggaggaaga
     5941 taatttggat actgccaaaa tgtgggaaga aggttggtta aaaaaacaag gggatatcta
     6001 tatcctcagg atgtttatgc cttttccgct caaggaaaac aactgcttta tcatcgaagg
     6061 cgaggatggc tgggttgttc ttgacacagg agtcagtctg gaacaaaacc aggatttgct
     6121 gaattctgcg ctcaggtcca tcggcatcac cttgcgccgg ctgaaggcag tttatctgac
     6181 tcattatcat catgaccaca gcgggctggc gggttggctg caagctaagg ccgacatacc
     6241 ggtttatctt cctcaggaag atctggtcac ctggcagcgc tttattgata ccgaccaata
     6301 ttttgacagt gccataaccg aatgtcaaca ggagggctgg ccggaagagt atgttcatga
     6361 actgagcgat aacatccgct ttattaacac ctatcttcag cctttgcccg aatttatccc
     6421 ctactccgac ggcctgaccg tcaagctgca cggtgatgat tacctgacca tcgctgtgcc
     6481 gggtcatacc gacggacata tggtctatta caaccctgcc cgcggcatcc tctttcccgg
     6541 agacaatgtg gtcgaccaca ccatcctgca tttaaccgat tggccgcata cctatttgct
     6601 gaatccctgt gacctccacc tgcaggccct gacagggtta aaaaaactgc cggtggaact
     6661 cgtcattccg ggtcacggcc ggacgtttgc caatctgaac gacaagatcg atttgatcgt
     6721 cgcccaccat gagaaacgta agcgcatcgt ttatgaagca gtcaaaacgc cctccactgc
     6781 ctgggaaatt gctgccgatg tctttaagcc caaccagtac atccacattc gccgcctggt
     6841 gctggcagaa accctgggct acctggcttc actgctgaac gaaggccggg tggagcggga
     6901 gctgagcagc ggccaataca tctaccgacg cagctaatca acgccctgga ccatcgctaa
     6961 aaaatttaag ttgcctattg cataccaatc atttctgtat gtatcataat aatatattcc
     7021 cctttcgtat ataggtcgcg gatcttattg accggtaaga aatggcctcg tcgtgtggcc
     7081 gcgaggctat ttctctccga tcatatgatt acgaaaggtg tggaacacat gcagcgcaaa
     7141 gtgatgactg gtcacaaatc gggtttattt attcttctgg cacttttctt ccttatttct
     7201 tgtctcatca tgccaccacc agcaacatgc caggcacagg atactgcgga cacaactctc
     7261 accgggcgaa tcaccgcggc caataccggg gcaggtatcg gtgcggtttc ggtgcgcctg
     7321 tcgagtcttg ccaacgaaac actctatgag gtaaaaacag atgattttgg tttttaccgg
     7381 atggacgggc tggaggcggg ctcgtatatc gtcacggccg agcatcctgc ctatcaaacc
     7441 gcccaggaaa taaattttgc ggtcaatccc ggcgccaact gctgggactg ggcgctgcag
     7501 ccgatcagcg gtggtgcatc gctgtttgat gtttacgtcg aagtggtctg tgccacttcg
     7561 ggcattcacc tggcggatgt gccggtcgcg attaccgtga aaagagacag cgacaatcag
     7621 tttgttacat acaaccaggc cacggatgcg gacggagtag cgcatctgac aggcatgccg
     7681 gcgggcgttt atactttcac gattaattat gacatcccga acgtggccgc caagatcggc
     7741 ggctgggacg gttacatcag cgttccgttt gaggatatct ccggccccca ctgggccgat
     7801 gtgcggctga aacctatcta caaccagctg aacgtgacgg tcaagggcta cgatgtggtg
     7861 aaggatcagg cggacatgcc gctgggcggc gtcatcgtgg aggcttcggg ctgtgacccg
     7921 gccgatgaca gcaatgtcct gctgccgccc caggtagggg tgagcggtct gaccaaggat
     7981 gctccctact gggacaattc gatggccggc aaggtgaagt ttgacaagct gatccccctg
     8041 acctggctgt tggaaacgaa aaagctgggc tacgagaaca agaatctttt cgtgcgctcg
     8101 aacagcagcg gcggcctgat gcaaacggac gttaccatca atattcctct ggaagacacc
     8161 aagctgtggg tgtggctgga ctccccctac aatgacccgg agatgctgga ggggcttgac
     8221 gtgatcctgg aggggatcac cgggcaaaat acggaaggca tcgcccgccg ggtgaaggca
     8281 gtgtacagta cggaagaaga cagtacctac gcgcttttca acaatatcct gccgggcaat
     8341 tatgatgtca gagtggatgc caagattgat aaaacagttc ctattgaagt ggacggcaag
     8401 aaaatttatg acggtcttta cggtgcaccc tcagacaagc agtttacggt gcgctttacc
     8461 gcccaggaaa cgttttatgc ggaaaatgac aaaaatacgg atgtaacgct gcagttgacg
     8521 ccggaaaaga tgaagctctc cggagtcctt tgcacagtgg acggggaaag agcgacggac
     8581 agcgcttatg cccagtatgc gggcgcgccg gcggccggca aaaatgtgga gattcgtctg
     8641 tcggagtata tgaacacagc ttttcccgcc gaggaaaaac tgcatcagac ggtgacggat
     8701 gccagcggca atttctcagt ggaactgctg cccggcctgt acgggatggt ggttacgggg
     8761 atggacgatt actggggaaa ttctgcagat ctcagcgatg ctgcgcatcc ggaccagtgg
     8821 ctttacactt atcacgcctg gccttattac caaatgtggc catacagcta tgaatcagcc
     8881 ctggccttca aggaccggag tccccaaaac aatgaaccga tgtctgatat cggcgggatg
     8941 ccggtttcct ccgggcagac gctgacaggg acgtttgtcg tgcgcaagga tgttttcaat
     9001 tatgatgtgg acatcaaacg tggcctgcag gatcccttcg aacatatggt agtgggcatc
     9061 gaccgcagcg gcagttcgtc caaggtgatc acccatacct atgatatgat caaaaacggt
     9121 ggacaactga ccctgaacgg tccggacagc cagatccagg ccgttgatct gggcacgacc
     9181 gagccttcct ttaaaaatct cccggcggga caatactcac tgacgctgac ccatccgcgc
     9241 tacacccaga tttggacgcc gacgacgacg ttttttgatt ttccctctcc gggtatgctg
     9301 ccatctgtag ccttcccgga gaattacaac agctttcaat ctcccgggcc tggtccctgg
     9361 ccactgctgc cctggagtgc ctttttccgg ccggaatacg ccatgaccgg ggcaatccag
     9421 gtgtcaaaca tgatgacgga ggaacaccgg gtgcgggtaa tgaaatggac ccagatcagc
     9481 agcgatccgg tggagtggga ttatgttgag gttaccgggc aggcttatcc gcgttttatc
     9541 aaaactgcct ataccggtga tctgctgttt gaaacggaac gcacgatgaa tgacccgacg
     9601 gcactcgcca ccaccgctta tgtaccaaac accaattatg atgcctattt ttggtggccg
     9661 gcggggaaca gtctcttcga tgacgctgcg ga
//
LOCUS       DAOTHZ010000071         9608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_8448_length_9608_cov_7.821417, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000071 DAOTHZ010000000
VERSION     DAOTHZ010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8448_length_9608_cov_7.821417"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /gene="ligA"
                     /locus_tag="P5549_08265"
     CDS             <1..1690
                     /gene="ligA"
                     /locus_tag="P5549_08265"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRW13105.1"
                     /translation="DTSGYMAELKIDGLTIALIYENGLLVKAATRGDGSIGEDVTLNV
                     RTIKSIPLKLHQPLPHLEVRGEVYLPKNEFVRLNQEREERGERVFANPRNAAAGSLRQ
                     QDPGVTAGRALAAFIYDITYIEGVRLDTQQEALAFLRNQGFPVNAEAAYYDRITDVYG
                     YCQRIEEQRHTLPYEIDGVVVKLNAFAARQVLGQTAKSPRWAIAYKFLAEEKETRLLE
                     IEINVGRTGIIAPTAIMEPVSLAGTTVSRASLHNFDLIRDKDIRIGDLVLLHKAGDII
                     PEIIRPLTEKRSGDEQVIIPPGNCPACGSQAVKTDGEVALRCENINCPARLKESLIFF
                     ASRSAMDIEGMGPALVEQLVEKELVSEIADIYSLTAERIADIERMGSKSAQNIVQAIE
                     ASKQRPLYRLLTALGIRHIGAKTARLLTREIKQMDDFYQMTEEQLLRIPEVGPKMADS
                     LVSFFAESRNREGLERLKQAGLNMVENNPADSQQPLAGKTFVLSGALTSMGRQEASER
                     IEALGGKVSSSVSKKTTYLLLGDEPGSKYDKAMQLGVTIMPEEEFLALLREYNE"
     gene            1719..2558
                     /locus_tag="P5549_08270"
     CDS             1719..2558
                     /locus_tag="P5549_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRW13106.1"
                     /translation="MGIRIVSDNCCDLPAEILRKYDIRLAHLQVRFGDNVYPPGEPTV
                     DEFYKILAESGVLPATSQPTVDNITKVYTEALADGSEVIAIHMSSALSGTYQTGVMVQ
                     NTLNNPRLHVFDSLRASLGIGLMVVEAAKMAQRGESVAAIMERLTGMRKTIQCIFVVG
                     NLDSLIRGGRLSKAKGKIAQVFDIKPILYMDDEGFLMPYDKARGLKGAQRKQIDIMEH
                     IGRSLERQTVGINHSAVPEIAEALCQTIKERFAVEEVIVNQIGPIIGSHVGAGTFSVY
                     FEK"
     gene            2625..3314
                     /locus_tag="P5549_08275"
     CDS             2625..3314
                     /locus_tag="P5549_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRW13107.1"
                     /translation="MTGTIINTAAIIMAGLLGLALRRGIPEQMARTMQDGLGILILVI
                     GLQYGFKADSLAIVGLSLALGAVIGEWRQWEAKLERLGEKLQSLTGGQNEQFVRGFVT
                     ATMVFCVGAMAILGSLEDGLTHNPQILIVKSMLDGIFSIIFAASMGVGVLFSAIPVFL
                     YQGAITLGASFLEPLMTDPLLNNVTALGGILIAGIGLNTLGITRIRLANLLPGLFIVP
                     LLMYIASLFKL"
     gene            3440..3724
                     /gene="gatC"
                     /locus_tag="P5549_08280"
     CDS             3440..3724
                     /gene="gatC"
                     /locus_tag="P5549_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRW13108.1"
                     /translation="MALTIKEVEYVAQLARLQLNETEKQSFAEQLSSILQYVEKLNEL
                     STEGVEPLAHILPVYNVFRVDESKPGTLREEILANAPLVEEGQYKVPKIM"
     gene            3752..5206
                     /gene="gatA"
                     /locus_tag="P5549_08285"
     CDS             3752..5206
                     /gene="gatA"
                     /locus_tag="P5549_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRW13109.1"
                     /translation="MELYQLTIHELQDLLSRKETSALEITQAIFARITAVDDRVRAFI
                     TLTEEEALAKAREIDQQGQYGRIAGIPLGLKDIFCTRGVKTTCASRILGNFIPTYDST
                     AAGRLFAEHGILAGKLNMDEFAMGSSTEQSAFFPTCNPWDLTRVPGGSSGGSAAAVAA
                     DEIPFALGTDTGGSIRQPASFCGVVGLKPTYGRVSRWGVVAYASSLDQVGTFAKDVKD
                     CALIMQIISGKDPLDSTSVDLPVPDYQTSLDGDVKGMKIAYPREYFQPGIDPNIQEII
                     KKALLKYEEMGAIVEEVSLPHSEYALPAYYLVATAEASANLARYDGVRYGLRDYEAAN
                     VIDMFSRSRAEGFGAEVKRRIMLGSYALSSGYYDAYYLKALKVRRLIADDFSRVFADF
                     DLIVSPTTPTVAFKLGEQIGDPLTLYMNDILTVPVNMAGLPAMSIPCGFHEGLPIGMQ
                     LIGPAFAESTLIKAAFAYEQNTDFHRLRPALEVK"
     gene            5209..6657
                     /gene="gatB"
                     /locus_tag="P5549_08290"
     CDS             5209..6657
                     /gene="gatB"
                     /locus_tag="P5549_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRW13110.1"
                     /translation="MSKDFEIVIGLEVHAELKTRSKIFCSCSTAFGAEPNTQVCPVCS
                     GFPGMLPVLNEQAVECAIKTGLALHCEIAEFCKFDRKNYFYPDLPKAYQISQFDLPIC
                     KGGYLDIEADGEKIRVGITRAHLEEDAGKLVHQGDITTTPFSLVDLNRSGVPLLEIVS
                     EPDMRSATQARAYMEKLRSILLFADVSDCKMEEGSLRCDANVSVRPRGQEKLGTRTEI
                     KNLNSFRALEKAIEYEAKRQIEAIEDGEEIVQETRTWDEAKGVTRSMRSKEEAHDYRY
                     FPEPDLPPLRISQDWVDKVRSSMPELPEQAGRRLMEQHGLSSYDVDLMTQSPQILQFF
                     DDTLRLHSDAKAVSNWMMGDLSRLLNQNGMEIQEAKVKPALLADMLNLLDEGTISGKM
                     AKTVFEEMFASGKDPQTIIKEKGLVQISDADALLPLIEEIVNNNPKVLQDYKNGKEKA
                     FGFFIGQIMKATRGQANPEMVNKLLKERLDRE"
     gene            6662..7834
                     /gene="nifV"
                     /locus_tag="P5549_08295"
     CDS             6662..7834
                     /gene="nifV"
                     /locus_tag="P5549_08295"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HRW13111.1"
                     /translation="MTWTMANKDWFLEKEAKIYIVDTTLRDGEQTAGVVFSNEEKIRI
                     AQYLDGMGIDQIEAGIPVMGGDEKKCITEIAHMGLKSSIMAWNRAVIADIKESLDCGV
                     DAVAISISTSDIHIQHKLQTTREKVLHMMSDAVKFAKDNDLYVSVNAEDASRSDLDYL
                     TEFALIAKHAGANRLRFCDTVGTLNPLTTFRYIKTLHDAVGLDIEMHTHNDFGMATAN
                     SLSGVYGGANHVGVTINGLGERAGNSCLQEVIMGLKHLMNVDVNYDTTIFREVAQYVA
                     HASGRELPVSKPIVGSNIFAHESGIHGDGVLKNPLTYEVFKPEEVGLERQIIIGKHSG
                     TAAIKSKFKEYLVNLTEEEAKEILARVRQMSIDKKRSLFDKEVVYIYEEIKRERNK"
     gene            7834..9090
                     /locus_tag="P5549_08300"
     CDS             7834..9090
                     /locus_tag="P5549_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRW13112.1"
                     /translation="MGKTIAEKILSQKSGQDARANDIVVAELDFVMGQDGTSGLAIRA
                     FEEMNSTRVFDPAKIAMVIDHSAPSPLEGVSALHQQMRVFADQQKINFYDIGDGVCHQ
                     LVPEQGHVVPGNLVIGADSHTCTYGAINVFSTGVGSTDLAAGLSSGKLWFKVPETFKF
                     VINGKLPPGVHSKDLILYLIGDVTADGATYMAAEYTGEAIAALSIEGRFTISNMAIEM
                     GAKCGLMEADEKTVAWVNAHSNKPFTPVQADPDATYARVKEYNVSKLEPQVAKPHTVD
                     NVSPIGEVAGIPIQQGVIGTCTNGRIEDFRIAANILKGRKLAHGTRLIIAPSSRQVML
                     QAMREGLIEIFMEAGAAVVTPGCGPCVGTHNGVPSDGENVISTANRNFKGRMGNNKAF
                     IYLASPATVAASVLYGKISDPREFNS"
     gene            9102..9602
                     /locus_tag="P5549_08305"
     CDS             9102..9602
                     /locus_tag="P5549_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRW13113.1"
                     /translation="MNLQGKVHKFGNDVNTDYIISGRHKFKTLDMKELAKHVMEDLDP
                     EFYNKIDRGDFIVAGSNFGCGSSREQAPLAIINADISAVIAKSFARIFYRNCINTGLP
                     LIECDTDLINPGDALEIDLGAGILHNLTQNIDIPITPLPPVMLKILNDGGLVEHFKKY
                     GGFNFD"
BASE COUNT         2500 a         2200 c         2600 g         2308 t
ORIGIN      
        1 agatacgagt ggttatatgg cggaacttaa aatagacggg ctgaccattg cgctcatcta
       61 tgaaaacggg cttctggtca aagccgcaac ccgcggggat ggatccattg gtgaggatgt
      121 gaccctgaac gtcaggacca tcaaaagcat tccgctcaaa ttgcatcagc ccctaccgca
      181 tctggaggtg cgcggggaag tgtacctgcc caagaatgaa tttgtcaggc tcaatcagga
      241 gcgggaagag cgtggcgaaa gagtttttgc caatccccgc aatgcggccg ctggctcatt
      301 gcgccaacag gacccgggcg taactgccgg gcgggcgctg gctgctttta tctacgatat
      361 aacctacatt gaaggagttc gccttgatac ccagcaggaa gcattggctt ttctgcgtaa
      421 tcagggtttt ccggtcaatg cggaagcagc gtattatgac cggattaccg acgtatatgg
      481 ttactgccag cgtattgaag aacagagaca taccttaccc tatgagattg acggggttgt
      541 tgtaaaactg aatgcttttg cggcccggca ggttttgggg caaacagcca aaagtccgcg
      601 ctgggcgatt gcttacaagt tcctggcaga agaaaaggaa acccggctgc tggaaataga
      661 aataaacgtt ggccgaacag gaatcatagc ccctaccgcc atcatggaac cggtcagcct
      721 ggccggaaca actgtcagcc gggctagttt gcacaatttc gatttgatca gggacaagga
      781 tattcgcatc ggtgatctgg tgctgctgca caaagccggc gatattatac ccgaaatcat
      841 cagaccgcta acagagaaac gcagcgggga tgaacaggtc ataattccgc cgggaaattg
      901 tcctgcctgt ggcagtcagg cagtcaaaac cgacggcgaa gtggctctgc gctgtgaaaa
      961 catcaattgt ccggctcgtc tcaaggaaag tcttatcttt tttgcttcgc gttcggccat
     1021 ggatattgaa gggatgggac ctgccctcgt tgaacaactg gttgaaaagg aactggtcag
     1081 tgagattgcg gacatatact ccctgactgc ggaaaggatt gccgacattg aacgcatggg
     1141 cagcaaatca gcccaaaata tcgtccaggc catcgaagca tcaaaacaaa gaccgctgta
     1201 ccgccttttg accgcgctgg ggatcagaca tatcggcgcc aagactgccc gtctcttaac
     1261 ccgggagatc aaacagatgg atgattttta tcaaatgacc gaggaacagt tgctgcgcat
     1321 cccggaagtc ggaccgaaaa tggcagacag tctggtgagt ttttttgccg aatcacgtaa
     1381 ccgggaaggg ctggaaagat taaagcaggc tggcttgaac atggtggaga ataacccggc
     1441 cgacagtcag cagccgctgg ccggcaaaac gtttgtgctc agcggtgccc tgaccagcat
     1501 gggacggcag gaagccagtg aaagaattga ggctttgggc gggaaagtga gcagcagtgt
     1561 tagtaaaaaa accacctatc tgctgctggg cgacgaacca ggcagtaaat atgacaaggc
     1621 catgcaatta ggtgtaacaa tcatgccaga agaggaattt ctcgcgctgt tgagggaata
     1681 taatgaataa aattatgaat tgctaaggag agctgcgtat gggaatcagg atcgtatcgg
     1741 acaactgttg tgatctgcct gccgaaatat tgaggaaata tgatattcgc ctggcgcatc
     1801 tgcaggtcag atttggggat aacgtatatc cgccgggcga accgactgtg gatgagtttt
     1861 acaagatatt ggcagaatcc ggtgtattgc ctgctaccag tcaaccaacg gtagataata
     1921 tcaccaaggt ttacactgaa gccttggctg acggttcgga agtcatcgcc atacatatgt
     1981 cttccgccct ctccggtact tatcaaaccg gggtgatggt ccagaatact ttaaataatc
     2041 cccggttgca tgtatttgat tccctgcggg cttcattggg gataggttta atggtggtgg
     2101 aggctgccaa aatggcacaa aggggagaaa gtgttgctgc cattatggaa cgtctgaccg
     2161 ggatgcgcaa aactatccag tgtatttttg tggtagggaa tctggatagt ttgatcagag
     2221 gcggacgact ttccaaggcc aaaggaaaaa ttgcccaggt atttgatatc aaaccgattt
     2281 tatatatgga tgatgaaggc ttccttatgc cctatgataa agcgcggggt ttaaaaggcg
     2341 cccaacgcaa gcagattgat atcatggagc atataggcag aagtcttgaa cgtcagacag
     2401 ttggtattaa tcacagtgcc gttcctgaaa ttgccgaagc attgtgccag acaatcaagg
     2461 aaagatttgc ggtagaagaa gtgatcgtaa accaaatcgg accaatcatc ggttctcatg
     2521 ttggggcagg gactttttct gtatattttg aaaagtaaac tatctttttg tctcccgcct
     2581 ggagtataat cttttttata taaaaaggcg ggagttgtgt tgtcgtgacc ggaaccatca
     2641 ttaatactgc tgcgatcatc atggccggat tgctgggtct tgcgctgcgt cggggcattc
     2701 ctgaacagat ggctcgtacc atgcaggatg gattgggtat cctgatcctg gtcatcgggt
     2761 tgcaatacgg gtttaaagcc gacagcctgg ctattgtcgg gctaagcctg gcactgggtg
     2821 cggtgatcgg tgaatggcgg caatgggaag ccaagctgga gagactgggg gaaaaactgc
     2881 aaagcctgac cggcgggcag aatgaacaat tcgtaagagg ctttgtaacc gcgacgatgg
     2941 ttttctgtgt cggtgccatg gccatcctgg gatcgctgga agacggtctg actcacaatc
     3001 ctcagatttt aatcgtgaaa tccatgctgg atggcatttt ctccattata tttgctgcca
     3061 gtatgggagt tggcgttttg ttttctgcca ttcccgtatt tttataccaa ggagccatta
     3121 ccttgggggc ctccttcctt gaacccctca tgactgaccc cttgctgaac aatgtaactg
     3181 cactgggcgg aattctgatt gccggtatcg gcttgaatac tctggggatt acccgtattc
     3241 gcctggccaa tcttctgccc ggacttttta tcgtaccgct gctgatgtat atcgccagtc
     3301 tttttaagtt gtgattcctg gaccagaaga aggacgaaat ttgtatggtt ttaactgaac
     3361 catgcaaaat tatttattgt ctcccctgct ttttggtata ataatgggta attgttttat
     3421 tcggtaagga ggcggcaaaa tggcgcttac aataaaagaa gttgagtatg tggcccaact
     3481 ggccagattg caattaaacg aaactgagaa acagagcttt gctgaacaac tgagctcaat
     3541 cctgcaatat gtggaaaagc taaatgaatt gtcaaccgag ggtgtagaac cgctggcgca
     3601 tattctgccg gtatacaacg tttttcgtgt ggatgagagc aagccgggaa cgttgcggga
     3661 agaaatactg gccaatgcac cgttggtgga agaaggccag tacaaagtac cgaaaatcat
     3721 gtaggtgcat cgaaacaagg aggttgccag cttggaattg tatcagctta ccatccatga
     3781 actgcaagac ttgctaagcc gtaaggaaac ttccgccctg gagattaccc aggcgatatt
     3841 tgcccgcatt actgcagtcg acgaccgggt gcgggctttc attaccctga cggaggaaga
     3901 ggccctggct aaagcacggg aaatcgacca acagggtcag tacggaagaa ttgccgggat
     3961 accgcttggc ttgaaagata tattttgtac ccggggggta aagaccactt gtgcttcccg
     4021 tattctggga aactttatac ccacttatga ttctacagcg gccggtcgat tgtttgccga
     4081 acatggcatc ctcgccggca agcttaacat ggatgaattt gccatgggat cttccaccga
     4141 acaatccgct tttttcccca cctgcaatcc ctgggatctc acccgggtac cgggcggatc
     4201 atccggtggt tcggcggcag cggtggcagc cgatgaaatt ccttttgctc tgggaactga
     4261 taccggcggg tcgattcgtc agccggcttc gttctgcggg gtcgtgggat tgaaaccgac
     4321 ctatggcaga gtatcgcgct ggggcgtggt tgcctatgca tcttccctgg accaggtggg
     4381 cacctttgcc aaggatgtaa aggactgtgc actgattatg cagattattt ccggcaagga
     4441 tccgttggat tcgaccagcg tggacctgcc tgtaccagac tatcagacga gtttggatgg
     4501 agatgtcaaa ggcatgaaga tcgcttaccc gcgcgaatat ttccaaccgg ggatcgatcc
     4561 taatatccag gaaataatta aaaaggcgct gcttaaatac gaagaaatgg gcgccattgt
     4621 ggaagaagta tccttgcccc acagcgaata cgccctgcca gcctattatc tggttgccac
     4681 ggccgaggcc agtgcaaatt tggcccgtta tgacggggtc cgatatggat tgcgtgatta
     4741 tgaagccgcc aatgtgatcg atatgttttc tcgatccagg gcggagggtt ttggcgccga
     4801 agtcaagcgg cgcattatgc tgggcagtta tgctttaagc tcgggatact atgatgccta
     4861 ttatctgaaa gccttgaaag tcagacgttt gatcgccgat gatttttcca gggtatttgc
     4921 tgattttgat ttgatcgtat caccgaccac cccgacggtt gctttcaagt taggggaaca
     4981 gatcggtgat ccgctgacct tatatatgaa tgacatcctg acggtgccgg tcaatatggc
     5041 cggactgccc gccatgtcaa ttccctgcgg cttccacgag ggcttgccga tcggcatgca
     5101 attgatcggc ccggcatttg ccgaaagcac gcttatcaag gccgcctttg cgtatgaaca
     5161 aaacacagat ttccatcgct tgagaccagc tttggaggtg aaataaaatt gagtaaggat
     5221 tttgaaattg tcatcgggct ggaagttcat gctgagctga aaaccaggag caagatcttt
     5281 tgttcctgtt ccaccgcctt tggggctgag ccgaatactc aggtatgtcc ggtctgctcg
     5341 ggttttcccg gcatgctgcc agtgctcaac gaacaggcgg tggaatgcgc tatcaagacc
     5401 ggactggctc tccattgtga gatcgctgaa ttttgcaagt ttgatcgcaa gaattatttt
     5461 tatccggatc tgcccaaagc ctaccagatt tcgcagtttg atctgcctat ctgcaaaggc
     5521 ggatatctgg atattgaagc agacggggaa aagattcggg tcggcatcac ccgcgctcat
     5581 ctggaggagg acgccggcaa gctggtccat caaggcgaca ttaccactac cccgttttct
     5641 ctggtggatt tgaaccgctc cggtgttccg ctgctggaga ttgtttccga gcctgacatg
     5701 cgcagtgcca cgcaggcacg ggcctatatg gaaaaactcc gttccattct tttgtttgct
     5761 gatgtatctg attgcaaaat ggaagaagga tccctgcgct gtgatgccaa tgtttcagtg
     5821 cgcccccggg ggcaagaaaa actaggcacc cgcacggaga tcaagaacct caattctttt
     5881 cgggcgctgg agaaggccat tgaatatgaa gccaaacgtc aaatcgaggc cattgaagac
     5941 ggggaagaga ttgtgcagga gacccggacc tgggatgagg ccaaaggcgt aaccagatcc
     6001 atgcgttcca aggaagaggc tcacgattac cgctatttcc ccgaaccgga tctgccaccc
     6061 ctgcgcatca gccaggattg ggttgacaag gtccgaagca gcatgcccga attacctgag
     6121 caggccgggc gaagactgat ggagcaacat ggattgtctt catatgatgt tgatttgatg
     6181 acccaaagcc cgcagatctt gcagttcttt gatgatacgc tgcggctgca ttccgatgcc
     6241 aaagcggtat ccaattggat gatgggggat ttgtccagat tgttgaacca aaacgggatg
     6301 gagattcagg aggccaaagt taaaccggca ttgctggccg acatgctgaa cttgctggat
     6361 gagggcacaa tcagcggcaa aatggccaag acggtttttg aggaaatgtt tgccagcggc
     6421 aaggacccac aaacgattat caaggaaaaa ggcttggtgc aaatatcaga tgccgatgcg
     6481 ttgctgcctt tgatcgagga aatcgtcaac aacaacccca aggttttgca ggattataaa
     6541 aacggcaagg aaaaagcctt cggattcttt atcgggcaaa tcatgaaagc gacccgggga
     6601 caggcgaatc cggagatggt gaataaattg ctgaaagaac gtttagacag ggagtgatag
     6661 gatgacatgg accatggcca acaaagactg gtttttggag aaagaagcca aaatatatat
     6721 tgttgatacg accttgcggg atggtgaaca aacagcgggc gtcgtgtttt ccaatgagga
     6781 aaaaatcagg atcgcacaat acctggatgg aatgggcatc gaccaaatcg aagccgggat
     6841 tccggtgatg ggcggagatg agaaaaaatg cattaccgaa attgcccata tgggtctgaa
     6901 atccagcatc atggcctgga accgggcggt gatcgctgat atcaaggaat ctctggattg
     6961 cggagttgat gcggtggcca tatccatatc aacttccgat attcatattc aacacaagct
     7021 gcaaaccacc cgtgaaaaag tactgcacat gatgtccgat gcggtaaagt ttgccaagga
     7081 caatgatctt tatgtttctg taaacgccga agatgcatct cgttccgatc tcgactacct
     7141 gacggaattt gccttaatcg ccaaacacgc cggggctaat cggttgcgct tctgtgatac
     7201 ggtgggaacc ttaaatccgc tgacaacttt tagatatatc aagacgctgc acgatgcagt
     7261 aggattggat atagaaatgc atacccataa tgatttcggc atggccacgg ccaactccct
     7321 ctccggggtg tacggcggtg ccaaccatgt gggcgttacc atcaacggtc tgggagagag
     7381 agcaggcaac tcctgtctcc aggaagttat tatgggattg aagcatttaa tgaatgtcga
     7441 cgtgaattac gataccacta ttttccgtga agtcgcccag tatgttgccc atgcttccgg
     7501 gcgcgagctg ccggtcagca aaccaatcgt gggttccaat atttttgcgc atgaatcagg
     7561 cattcacggt gacggcgtgc tgaaaaatcc gctgacctac gaagtcttta aaccggagga
     7621 agtgggcctg gagcggcaga tcatcatcgg caagcattct ggtacggcgg ccatcaaatc
     7681 aaagtttaaa gaatacctgg tcaatctgac agaagaagaa gccaaagaaa tcctggcccg
     7741 ggtgcggcag atgtcaattg acaaaaagag gtcattattt gacaaagaag ttgtctatat
     7801 ttacgaagaa atcaagaggg agaggaacaa atagtgggga aaacgattgc tgaaaaaata
     7861 ttgtcacaaa aaagcggtca ggatgccaga gccaatgaca tcgttgttgc ggagctggac
     7921 tttgtcatgg ggcaggacgg gacttccggc ctggctatca gagcctttga agagatgaat
     7981 tccacccggg tttttgaccc ggccaaaatc gccatggtga ttgatcacag tgcgcccagc
     8041 ccgctggaag gggtttccgc cctgcaccag cagatgcggg tatttgccga tcaacaaaaa
     8101 atcaattttt acgatattgg cgatggtgtc tgccatcagc tggtgcctga acagggacat
     8161 gttgtgcccg gaaatctggt catcggggct gattcgcata cctgtacgta tggtgctatc
     8221 aatgtttttt ccaccggggt gggatcgact gatctggccg ccggtttaag ttctgggaaa
     8281 ctttggttta aggtgcccga gacttttaaa tttgtcatca atggcaaact tccgccgggg
     8341 gtacattcca aggatttaat cctttatctg atcggagatg taaccgccga tggcgctacc
     8401 tatatggcgg ctgaatatac cggcgaggcc atcgctgcgt tatccattga gggccggttt
     8461 accatcagca atatggctat tgaaatgggt gccaaatgcg ggctgatgga agccgacgaa
     8521 aaaactgtgg cctgggtaaa tgcacacagc aacaaaccgt ttacaccggt tcaggccgat
     8581 cctgatgcga catatgcccg ggtgaaggaa tacaatgtat ccaaacttga accccaggta
     8641 gccaaacccc atactgttga taatgtatcg ccgattggcg aagttgccgg gattcccatt
     8701 cagcagggtg tcattggtac ctgtaccaat ggacgaatcg aagatttccg gattgccgcc
     8761 aatattctca agggcagaaa gctggctcac ggaactcgtt taatcatcgc cccttcctcc
     8821 cgacaggtga tgctgcaggc catgcgcgag ggactgatcg aaatctttat ggaagccggg
     8881 gcggcggtcg taaccccggg ttgcgggcct tgtgtgggca cccacaacgg agtcccctct
     8941 gacggagaaa atgttatttc cactgccaac cgcaatttca agggcagaat gggtaacaac
     9001 aaggcattca tctatctggc gtcgccggcg acggttgccg cttcagtttt atacggcaag
     9061 atcagcgatc cccgcgaatt taacagctag gaggcttgaa catgaattta caaggcaaag
     9121 tacacaagtt tggcaatgac gtcaacaccg attacatcat ttccggccgc cacaaattta
     9181 agaccctgga tatgaaggaa cttgccaaac acgtgatgga agatttggat ccggagtttt
     9241 acaacaagat cgaccgcggt gattttattg tagccggcag caattttggc tgcggttctt
     9301 cccgcgaaca agcacccctg gcaatcatca acgcggatat tagtgcggta attgccaaat
     9361 cgtttgcccg cattttttat cgcaattgca ttaatacagg gctgccgctc attgaatgcg
     9421 atactgattt aatcaacccc ggagatgcgc tggaaataga cctgggtgcc ggtatcctgc
     9481 ataatctcac ccagaatatt gacatcccca tcacaccgct gccgccggtg atgctgaaaa
     9541 tcttgaatga cggcggactg gtggagcact ttaaaaaata tggcggattc aattttgatt
     9601 aaagcgat
//
LOCUS       DAOTHZ010000072         9224 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_8951_length_9224_cov_7.201222, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000072 DAOTHZ010000000
VERSION     DAOTHZ010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8951_length_9224_cov_7.201222"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="P5549_08310"
     CDS             <1..305
                     /locus_tag="P5549_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRW13114.1"
                     /translation="DLVSFGVAPALLIYAQVLDPYIYSLGIFAAVFFIVCGAFRLARF
                     NVLNISDYFVGIPITLAGFLAAILSLLAAWLPGNLILALMLLLAILMVSNFKVRKF"
     gene            360..977
                     /locus_tag="P5549_08315"
     CDS             360..977
                     /locus_tag="P5549_08315"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRW13115.1"
                     /translation="MALPDLEFLKHRKQGILGENQSFRSAVFLPLVELKDQPHVLFEK
                     RAADLKVQPGEICFPGGAIDKNDASPAQAAIRETSEELKIACDDIELIAPLDIIASPF
                     NAIVYPFVGFIHNPQKISFNPSEVEELFCVPLQHLIDQDPLKYVLHLAVQMPEDYPYE
                     LVPGGKNYPYRTGKMPQQFYFWEDRIIWGLTARILHHLLELLNAK"
     gene            1100..1660
                     /locus_tag="P5549_08320"
     CDS             1100..1660
                     /locus_tag="P5549_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014519730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRW13116.1"
                     /translation="MKKGWIIALVVLVIVIFSGVGSYNSLIAMNEKVDGNWSQVENQL
                     QRRADLIPNLVSTVKGFAAQEKDILNTIAESRSKLIGAGGVADKAAVNDELNSALSRL
                     LVVVENYPNLKSDANFRQLMDDLAGTENRLAVARKDYNDSVQVFNAKIKSFPNVIWAG
                     MLGFETREYFKAAESANQTPIVDFEK"
     gene            1669..2496
                     /locus_tag="P5549_08325"
     CDS             1669..2496
                     /locus_tag="P5549_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRW13117.1"
                     /translation="MTKRLLPKQKHPARLNYKKRLGRGSLLSLLLLFCLLSLPAQAVD
                     IPSPPADLYVLDQAAVMSPETRSLIISTSQDLARQTTAQVVVVTLDTLEDQVLEEVSL
                     TILREWGIGDKELNNGVLILLVPSEHKSRIEVGYGLEGVLTDGKTGRIQDEYMLPYYK
                     QEQYDLGLRNGYLAVVQEVAQEYNVTLTPSQGYHATPGETEAGNEPTPTWLKVLGIFL
                     LILLIWLDHKFLNGFILGMILSGMFRGGGRGGGGGFGSGGGGFGGGSGGGGGSSRGW"
     gene            2640..4271
                     /locus_tag="P5549_08330"
     CDS             2640..4271
                     /locus_tag="P5549_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW13118.1"
                     /translation="MERSWYAFYDEGVRHSIDYPGKPMKDYFSKWVEKNPDKPYLIWG
                     DKQLTYKEANSIACKLASSIIGLGYVKGDRIALMSTNLPEYVICLQACWKLGAAIVPT
                     NPMYTVPELTHQFGDSGAKFVFSEAQFANKVVEIIKNGKTSVQKLVVIQTANKVEFEP
                     ADYLFDYEALAAQGEDREPDIEVFSEDLSMLQYTGGTTGLSKGCLLSNANLEAMAFQD
                     VEWFKPPFHNPEEIVAMAAMPLYHIYGFNTSVNMMMVAGGTIIVVNGPTPDNLLKNIN
                     EHEPNYFAAVPAMIYGLNNHPDISKSKIKSIKAMICGSSPLAVEGLNKFEELSGAVIT
                     EGYGLSETSNVLTCTPFYTKRKIGSVGLPWPDVDIKIVDLEEGTKEMPNGEPGELIAK
                     GPQIMSGYWNNPEETANVLRDGWLYTGDIAAIDDDGFITIVDRKKDMILCSGFNVFCR
                     EIDEVMYTHPKVLDACAIGIPDPKRGETPKIFIILKPGQTMTEDEVKEYCRKYLAPYK
                     VPTHVEFIDELPRTSVGKPMRNALRQQEKAKNNQS"
     gene            4306..4878
                     /locus_tag="P5549_08335"
     CDS             4306..4878
                     /locus_tag="P5549_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRW13119.1"
                     /translation="MEFFDVVFNRRAVRQYKPDPVSREDILKIIEAASYAPTGQNLQQ
                     VDYVVVTGQAKQKLGESYGVIGVAYSKDWEDEASRRNFVEYAQTYGGAPAIIVVLTDT
                     SKHDAVRKMHLESGSAAMENLCLAARALGLGTCWMTGPLQDEAAIRKVLDISEKKEIV
                     AVTPLGYPVEFPPAPPRIDPELKQKIRWVE"
     gene            5067..6599
                     /locus_tag="P5549_08340"
     CDS             5067..6599
                     /locus_tag="P5549_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW13120.1"
                     /translation="MQEAKTQEQLLLEIESLKARIAEIETFNQMDEDGYASMVRALRL
                     SEEKFSNAFHYSTDIMTISSLQDARYVDVNDAFLNIVGFPREEVVGHTSYEIDIWQYP
                     VDRERLMQQLKENGRVRNLEFVFRNRYGDLVTTLLSADIIKVEGKDCLLCVVKDFTER
                     KRIEHDLILSEQRFNKAFNFSPVTMSITTLEEGRFVDVNESFCQVLGYSRSFLLSRTS
                     VDIGFWVNPEQREEVKRILEKGGSVREMEVYFRRENGENRLGLYSAERIELDGEICIL
                     SQFIDLTERRHMEKEMTRLGQLNLVGEMAASIAHEIRNPMTTVRGFIQLLKENEQYAA
                     EKESFHLMIEELDRANAIITEFLSLAKNKMVEIKAVNINTILKNIYPLMLASAMMQDK
                     MISLDLEGIPDLLLDEQEIRQLILNLINNGLESIQEGKAVFVSTYTTEGKVVLAVRDQ
                     GTGIKKENFDKLGTPFFSTKEGGTGLGLAVCYGIASRYNAKIEIETGPRGSTFYVCFP
                     LV"
     gene            6780..7517
                     /locus_tag="P5549_08345"
     CDS             6780..7517
                     /locus_tag="P5549_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRW13121.1"
                     /translation="MMNRLVVDRESQIYQLLLDFRNGNKAALDDIFEQLMPFCLRVCS
                     KTCGKYIYDNDDEASIARLALVEALEKFDPAKGTMMVFIGQVIRNRVIDYKRKEQKQS
                     IISFKLFSRGEDRFEQEADESFFEEIIDSLARKQEIDALQGLLQPFNIAFHELPALSP
                     QQDKTRLSAQQIARIIANDDELRNHLLKKKTLPLKELETSWQINRRLADRYRKYILVT
                     ALIYMHDFPLLQEYILPKKGGRADENK"
     gene            7504..8283
                     /locus_tag="P5549_08350"
     CDS             7504..8283
                     /locus_tag="P5549_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13122.1"
                     /translation="MRTNKGIVIEANQKHAIILLPGGEYRKVRTGGKYLEPGDLYEQN
                     ATLLVKYAVAAVLVLTILVGGIDYHSVKAYARLGSDLEMGINRWGRVVTVEAKSEAAR
                     QILNAVEIENDKMEVAVEKIIRQAILDEKTSPQDVRRNLSVTAKEKEKQNPELEKKLQ
                     RAMEKGLEKAQPKANPGVDKKDQPKSEVKANPSVNKKTEPKAEVKANPSVNKKAEPKA
                     EVKASPGVNKKAEPKAEVKASPSENKKANPKAKATQVGDGS"
     gene            8552..9019
                     /gene="hutP"
                     /locus_tag="P5549_08355"
     CDS             8552..9019
                     /gene="hutP"
                     /locus_tag="P5549_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hut operon transcriptional regulator HutP"
                     /protein_id="HRW13123.1"
                     /translation="MRKRDWMSHLTKNLIGKNALLLVLADQEPEQEYGTIREELHWRC
                     CVGKVGAMEAHKVVAAIETAAKKNGIINPDLYREIHALYHAIVEAIEGVTRGQIQLGS
                     VLRTVGLRFSIIRGNPYDSPAEGEWIAVALYGTIGAPVKGLEHEVVGLGINHL"
BASE COUNT         2611 a         1995 c         2341 g         2277 t
ORIGIN      
        1 gtgacctggt atccttcggc gttgctccag cactgctgat ttatgcacag gttcttgatc
       61 cctatatcta ttcgctgggg atttttgctg cggtcttttt cattgtgtgc ggtgcctttc
      121 ggctggcgcg ctttaatgtt ttgaatatca gtgattattt tgtgggaatt cccatcaccc
      181 tggcgggttt tctcgctgcg atcctgtcat tgctggcggc ctggctgccc ggcaatttaa
      241 ttttggcttt gatgttatta ctggccatac tcatggtaag taattttaaa gtgcgtaagt
      301 tttaagtgaa ttagaatcct tccagcatcg gatggataaa ttcggaggca agagttttca
      361 tggcattacc tgatttggaa tttttgaagc atcgtaaaca ggggatatta ggcgagaatc
      421 agtcttttcg ttcagcggta ttcttgccgc tggtggaatt gaaagatcaa cctcatgtat
      481 tgtttgaaaa aagagcggcg gatttaaagg tgcagcccgg cgagatctgt tttcccggcg
      541 gtgccatcga taaaaatgat gccagtcccg ctcaggcagc gattcgagaa accagtgaag
      601 aattaaagat cgcctgtgat gatatcgaat taattgcccc cttggacatc attgccagtc
      661 cttttaatgc catcgtttac ccctttgtgg gctttattca caatccgcag aagatatctt
      721 ttaaccccag cgaagtggaa gaactgtttt gtgtaccgct gcagcatctg atcgatcaag
      781 atccactgaa atatgtcctc cacctggctg tgcagatgcc ggaagattat ccctatgaac
      841 tggtgccggg aggtaaaaac tatccctatc gcaccggtaa aatgccccaa cagttttact
      901 tctgggagga ccgcatcatc tggggcttga ccgctcgaat actgcatcat ctcctggaat
      961 tgttaaacgc aaaataattt gatttctcaa tgcccctgct ccgtctggat gaggggcttt
     1021 tttcaacgga agagatcgga ttgacaattt caagcaattg gatatgataa aaaaagactt
     1081 aatcaaggga ggcgttaatg tgaaaaaagg ctggattata gccctggtgg ttttagtgat
     1141 tgtgattttc agcggggtgg gcagttacaa cagtctgatt gcgatgaacg aaaaagtaga
     1201 cggcaactgg agccaggtgg agaatcagtt gcagcgccgg gcagatttga ttcctaatct
     1261 ggtatcaacg gtaaaaggct ttgctgcaca ggaaaaggac attctcaata ccatcgcaga
     1321 atcccgcagc aaattgattg gtgccggggg cgtagctgat aaagcggctg tcaatgatga
     1381 actcaactcg gctctgtcac gtttgctggt ggtggttgaa aattatccca accttaaatc
     1441 agacgccaat ttccggcagt tgatggatga tctggccggc acggaaaacc gcctggcagt
     1501 ggccaggaag gattacaatg attccgtgca agtcttcaac gccaagataa aaagctttcc
     1561 caatgtaatt tgggcgggaa tgctgggctt tgaaacccgt gaatacttta aggcggctga
     1621 atcggccaat caaaccccga tcgttgactt tgaaaaatag gtattcgtat gacaaagcga
     1681 ttgttgccaa aacaaaagca tccggcccgt ttaaactata aaaagcgctt gggcaggggg
     1741 agtttgctct ccctgctgtt gttgttttgc ttgctgtcgc tgccagctca ggccgtggat
     1801 attccgtccc cgccggctga cctttacgtg cttgatcagg cagccgtgat gagtccggag
     1861 actcgttccc tgattatcag taccagccag gatttggccc ggcagaccac agcccaagtg
     1921 gtggtcgtga cccttgacac ccttgaggat caggtgctgg aagaggtatc gctgaccatt
     1981 ctgcgggaat gggggattgg ggacaaggaa ttaaacaatg gtgtattaat cttgctggtg
     2041 ccttccgaac ataaatccag aattgaggtt ggctatggtc tggaaggcgt tttgacagac
     2101 ggcaagacgg gacgcatcca ggatgaatac atgcttccat attacaaaca agaacaatat
     2161 gacctcggct tgcgcaacgg ctatttggca gttgtccagg aagttgccca agaatataat
     2221 gttaccctca cacccagtca aggttatcat gccacccccg gagaaacaga agcgggcaat
     2281 gaaccaaccc caacctggct caaagtgctg ggcatcttcc ttctgattct gctgatttgg
     2341 ctggaccata agtttttaaa cggttttatc ctggggatga ttctcagcgg catgttccgc
     2401 ggcggcggcc ggggcggggg tggcggcttc ggcagtggcg gtggcggctt tggcgggggt
     2461 tccggcggcg gcggaggcag cagtcggggt tggtagaaaa atgattgttc tgttcaagtg
     2521 actcaatctt tccacaatca gtattgccgt tacaagcatc ttttgcatga aaaatccgca
     2581 taataacgaa ggatgtatta taatggtatt actagatttt ggatgggagg ttttcgtgaa
     2641 tggaaagaag ctggtatgca ttttacgatg aaggagttcg tcacagcatt gattaccccg
     2701 gaaagccaat gaaggattat ttcagcaaat gggtcgaaaa aaatccagac aagccctatc
     2761 tgatctgggg cgataaacaa ctcacctaca aggaagccaa cagcatcgca tgtaagttag
     2821 ccagttcaat tattgggttg ggttacgtca aaggtgaccg gattgcactg atgagcacca
     2881 acctgccgga atatgtcatc tgtctgcagg cctgctggaa gctgggcgcc gccatcgtac
     2941 ctaccaatcc aatgtatacg gtgccggaat taacccacca gttcggtgac agcggggcca
     3001 aatttgtttt ttccgaagcc cagttcgcca ataaggtagt cgagatcatc aagaacggca
     3061 aaaccagcgt acaaaagctg gtggtgatcc aaaccgccaa taaagttgaa tttgagccgg
     3121 ccgactacct ctttgattac gaagcattgg cagcccaagg ggaagacagg gaacccgaca
     3181 tcgaagtctt ttccgaagat ctttccatgt tgcagtatac cggcggcacc accggcttgt
     3241 ccaaaggctg tctgttgtcc aatgccaacc tggaagctat ggcatttcag gatgtagaat
     3301 ggtttaaacc acccttccac aatccggaag aaatcgtggc gatggccgcc atgccgctct
     3361 atcacattta cgggttcaac accagcgtca atatgatgat ggtcgccggc ggcaccatca
     3421 ttgtcgtaaa cggaccgacg cctgacaatc tgctgaaaaa catcaatgaa catgaaccta
     3481 attactttgc cgctgtacca gccatgatct acggcttgaa caatcacccc gacatcagca
     3541 aatccaagat taaatccatc aaagcaatga tttgcggttc ctcaccgctg gcagtagagg
     3601 gcctgaataa gtttgaagaa ctgtctggcg cagtcattac tgaaggatac ggactttcag
     3661 aaacgtccaa cgtgctgacc tgtacgcctt tttacaccaa acgtaaaatt ggcagtgtcg
     3721 gactgccttg gccggatgtg gatatcaaga tcgttgacct ggaagagggg accaaagaaa
     3781 tgcctaatgg tgaaccgggc gaactgatcg ccaaaggtcc gcagattatg agcggctact
     3841 ggaacaatcc ggaggaaacc gccaatgtcc tgcgggacgg gtggctttat accggagaca
     3901 ttgctgccat tgacgatgac ggtttcataa ccatcgtcga ccgcaaaaaa gacatgatcc
     3961 tgtgcagtgg ctttaatgtt ttctgccgtg aaatcgatga agtcatgtac acccacccca
     4021 aggttttgga tgcgtgtgcg attggcattc cggaccccaa acgcggcgag acgcctaaga
     4081 tatttattat tctgaaacca ggccaaacca tgaccgaaga tgaagtcaag gaatattgcc
     4141 ggaagtatct ggctccttac aaagtgccga cgcatgtgga attcattgac gaactgccgc
     4201 gtacttctgt cggcaagccg atgcgcaatg cactgcggca acaggaaaaa gccaaaaaca
     4261 atcagtcgta aaaaatgacc aattaaattg agaggagatg aaacgatgga attttttgat
     4321 gtcgtcttca atcgacgggc agtgcgccaa tacaaacccg acccggtcag ccgggaagat
     4381 atcttgaaaa ttatcgaggc agccagctat gcccctaccg gacagaattt gcagcaggtt
     4441 gattatgtcg ttgtaaccgg gcaagccaag caaaagctgg gagagagtta cggggtaatc
     4501 ggcgtggcct actccaagga ttgggaggat gaagcaagcc gccgcaattt tgtggaatat
     4561 gcccaaacat acgggggagc accggccatt attgtggtgc tgaccgatac cagcaaacat
     4621 gatgccgtgc gcaaaatgca tctggaaagc ggcagtgctg ccatggaaaa tctatgtctg
     4681 gcagctcggg ccttggggtt gggtacatgc tggatgaccg gacctttgca ggacgaggct
     4741 gccatcagaa aagtcctcga tatctccgaa aaaaaagaaa tcgttgccgt tacccccttg
     4801 ggttatccgg tggaatttcc acctgcgccg ccccgcatcg atccagagtt aaaacagaag
     4861 atacgttggg ttgagtaaat tacgttcgac ataatatccc atcaacaaaa ccgatcctcg
     4921 agacaggatc ggtttttgct tgaatcacga tgttattgtt cattcatgtc aattaatatg
     4981 ataaaattta atagataaca ataaaggtaa aagttagcaa cccgaataac ccttgcggca
     5041 ggaagttttg aaggaggtat aaccgcttgc aggaggcgaa gacccaggag cagctgctgc
     5101 tggaaatcga aagcttaaaa gccagaattg ctgaaataga gacgttcaat caaatggatg
     5161 aagatggtta tgcatccatg gtaagggctt tgcgtctttc ggaagaaaaa ttctccaatg
     5221 ccttccatta cagtaccgac attatgacta tttcttcctt gcaagatgca cgttatgtgg
     5281 atgtaaatga cgcgtttctc aatattgtcg gattcccccg ggaagaagta gtgggccaca
     5341 ccagttacga aattgacatc tggcaatacc cggttgaccg tgaaaggctt atgcagcaat
     5401 taaaggaaaa tggccgggta cgcaatctgg aattcgtctt tcgcaatcgt tatggtgatt
     5461 tggtgaccac cttgctctcg gcggatatca tcaaggttga gggaaaagac tgcctgctct
     5521 gtgtggtcaa ggattttact gaacgtaaaa gaattgaaca tgatttgatt ctgtcggaac
     5581 aacgtttcaa caaagccttc aatttcagtc cggttacaat gagtattacc accctggagg
     5641 aaggtcgttt tgtcgatgta aatgagagct tttgccaggt actgggttat tcccgatcat
     5701 ttcttctcag tcggacttct gtcgatattg gcttctgggt caatccggaa caacgtgaag
     5761 aggtcaaacg catcctcgaa aaaggtggtt ccgtgcggga gatggaagtt tacttccgcc
     5821 gggaaaacgg tgagaacagg ctgggacttt attctgccga aagaattgaa ttggatggag
     5881 aaatctgtat cctgagccag ttcattgatt taacggaacg ccggcatatg gaaaaagaaa
     5941 tgacccgcct gggacaactc aatctagttg gagaaatggc agccagcatc gcacatgaaa
     6001 tcagaaatcc tatgacgacg gtgcgcggtt ttattcaatt gttaaaagaa aatgagcaat
     6061 atgctgcaga aaaagaatcc ttccatctga tgatcgaaga attggatcga gccaacgcca
     6121 tcatcactga attcctttcc ctggctaaaa acaagatggt ggaaatcaaa gcagtcaaca
     6181 tcaataccat attaaagaat atttatccac taatgctggc cagtgccatg atgcaggata
     6241 aaatgattag cctggacctg gaagggattc ccgacttgtt gctggacgag caggaaatcc
     6301 gccaattgat cctcaacctc atcaacaatg gactggaatc tatccaggaa ggcaaggcgg
     6361 tgttcgtatc tacctataca actgaaggaa aagtggtttt ggcggtgcgt gatcaaggta
     6421 caggcatcaa gaaggaaaac tttgacaaac tgggtacccc cttcttcagc accaaagaag
     6481 gagggaccgg cctgggcctg gccgtatgtt atgggatcgc atcccgttac aatgccaaaa
     6541 tagaaattga aacaggtcca cggggcagta ctttttatgt gtgttttccc cttgtttaaa
     6601 ttcatttcga cctcaattaa cgccggttgc ttttcgtata tttgataaag caaccggcat
     6661 tcttattgtc aaaagagctg gttcttctca atcattgact tactcttttg tgaatagata
     6721 ataaactccc acagaggctg tgctacaata atagcagagt ttctgcaagg agcatttgga
     6781 tgatgaatcg tttggtggtt gacagggaaa gccagatata tcaattgctg ctggactttc
     6841 gcaatggcaa caaggcagcg cttgatgata tattcgagca attgatgcca ttttgtctgc
     6901 gggtttgttc caaaacctgc ggcaaataca tatatgacaa tgatgatgaa gccagtattg
     6961 cccgcctggc cttggtggaa gcgcttgaaa agtttgaccc ggccaagggt acgatgatgg
     7021 tttttatcgg tcaggtgata agaaatcgcg ttatcgacta taagcgcaag gaacaaaaac
     7081 aaagcattat ttcatttaaa ttgttctcac ggggcgaaga tcggtttgag caggaggcag
     7141 atgagtcttt ttttgaggaa attatcgatt cactcgccag aaaacaagaa atcgatgctt
     7201 tacagggatt gctgcagccg tttaatattg cttttcatga gttgcccgct ctgtctcctc
     7261 agcaggacaa gaccagactg agcgctcagc aaatcgcccg gattattgcc aatgatgatg
     7321 aactgcgcaa tcatttatta aagaaaaaaa cccttccgct gaaggaattg gagacaagtt
     7381 ggcaaatcaa tcgcagactg gccgatcgtt acagaaagta tatccttgta acggctttga
     7441 tctatatgca tgactttcct ctgttgcagg aatatatttt gccgaagaaa ggaggccggg
     7501 cagatgagaa caaataaagg aattgtcatt gaagccaatc aaaaacatgc cattattctc
     7561 ctgccgggcg gcgaatatag aaaagtgcgc acaggaggaa agtatctgga acctggtgat
     7621 ttatatgaac aaaacgctac tctgctggtc aagtatgcgg tagcagctgt tttggtgctg
     7681 acgattctgg ttggcggtat agattatcat tcggttaaag catatgccag actgggatca
     7741 gatctggaaa tggggataaa tcgctggggg cgggtcgtaa ctgtcgaggc aaaaagtgag
     7801 gcagctcgac agatcctcaa cgccgtagag attgaaaacg ataaaatgga ggttgcggta
     7861 gaaaaaatta tccgacaggc catcctggat gagaagacaa gccctcagga cgtacgccgc
     7921 aatttgagcg taactgccaa agaaaaagaa aagcaaaatc ctgaattaga gaaaaaattg
     7981 caaagagcaa tggaaaaggg attggaaaag gcacaaccca aagcgaaccc gggtgtagat
     8041 aaaaaagacc aacccaagtc agaagtcaaa gcgaatccaa gtgtaaacaa gaagactgaa
     8101 cccaaggcgg aagtcaaagc gaatccaagt gtaaacaaga aggctgaacc caaggcggaa
     8161 gtcaaagcga gtccgggtgt aaacaagaag gctgaaccca aggcggaagt caaagcgagt
     8221 ccaagtgaaa acaaaaaagc caatcccaag gcaaaagcca cgcaagttgg ggacggttct
     8281 tgacttgcgt gtgaagatcc aaccgagatt catttaatag gttcacaata tattggatcg
     8341 aaaacgaaag ccgacgaaag attgtacttt tattggcttt ccttttttag tcaatgataa
     8401 ttatccagca taagtctcac gatcagtgca aactcaatga acgatagcca ccaaccatgc
     8461 tcaccgggga acgttggtga ttctcctttg tcagattgtt ggccattgac attaatgggc
     8521 taaataccta gactaaaagt agtatttagt gatgagaaag cgagactgga tgtcgcatct
     8581 gaccaaaaac ctgattggca aaaacgcttt gctgctggtc ctggccgacc aggaacccga
     8641 gcaggaatat ggtaccattc gggaggaact ccactggcgc tgttgtgtgg gcaaagtcgg
     8701 tgccatggag gcccacaaag tggtggcggc gatcgaaact gcggccaaga aaaacggcat
     8761 cattaatccc gatctctatc gagagatcca tgccctttat catgccatcg tggaagccat
     8821 tgagggcgta acccgcgggc agattcaatt ggggtcggtg ctgcgaacgg tgggactgcg
     8881 cttttccatt atccgcggca atccctatga ctcgccggcc gaaggggaat ggattgcagt
     8941 cgcgctctac ggaacgattg gcgccccggt caagggtttg gagcatgaag tggtgggtct
     9001 gggcatcaac cacttgtagc gtaaacaatc gtgaataaat taataactta ataacttggt
     9061 gccaggcacc aaaagtgaaa gttattgaaa tagagcctat agtttaagtt attagggggc
     9121 tttattaaga aggtcatgga cttttttaat aaagcccctc tttattgtta aaaggaggtt
     9181 gaatttattg attactttaa ccggcaactc tctgaccata aaag
//
LOCUS       DAOTHZ010000073         9181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_9007_length_9181_cov_7.555117, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000073 DAOTHZ010000000
VERSION     DAOTHZ010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9007_length_9181_cov_7.555117"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2576
                     /locus_tag="P5549_08360"
     CDS             <1..2576
                     /locus_tag="P5549_08360"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRW13124.1"
                     /translation="TGQVAEIYDEGFIAKRMEVGAVIGAGPKSKVVRATPAPGDMVIL
                     LGGRTGRDGCGGATGSSVEHTEESIYTSGAEVQKGNAPTERKIQRLFRNPEASRLIKK
                     CNDFGAGGVSVAIGELTDGLLIQLDAIPKKYDGLDGTELAISESQERMAVVVAREHSG
                     RFKELADMENLEATEVAEVKADRRLKMVWRGQTIVDIARDFLDTNGVKQHIEVQVAEP
                     DADKNYFKNKPATISADLKTAWLNNLQDLNVCSQRGLVECFDSTIGAGTLLMPFGGHY
                     QATPGIGMAAKLPVEEGDTTTCTLMTYGYNAQLSKWSPFHGALYAVVEAVSKIVAMGG
                     DYKTVRLSLQEYFEKLGTDRCKWGKPFAALLGAYQAQMQLGIPAIGGKDSMSGTFMEL
                     HVPPTLVAFAVTAADTRNLISPEFKHPDSQVVWIPIARDANELPDFEALRTAYERVTQ
                     LIHTGQITAAHPVRGGGIAEVISKMAFGNRIGFAAQDHLWAEEEANARWFTPEYGSLV
                     VEMPAEVNVAAALDGVTCHHLGRTIQDEMIVIGRMSLSLQETQTVWEAPLENVFPTQT
                     EELTLTAPRVVSHAQRGRTKASLTVAEPRVLIPAFPGTNCEYDTRKRFELAGAQADIF
                     VIKNLTPAAVEDSIRDMAAMIRQSQIIAIPGGFSAGDEPDGSGKFIAAFFRSPRIRDE
                     VMELLKNRDGLMLGICNGFQALIKLGLIPYGEIRDMDETQPTLTYNRIGRHMSRMVQT
                     RIASVLSPWLANVEVGDIHQIAVSHGEGRFVASIEELEQMIANGQIAAQYVDDDGQPS
                     YDIRYNPNGSDYAVEAITSPDGRVLGKMGHSERIGTHLYRNIPLEKDQRLFEAGVNYF
                     K"
     gene            complement(2661..3503)
                     /locus_tag="P5549_08365"
     CDS             complement(2661..3503)
                     /locus_tag="P5549_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HRW13125.1"
                     /translation="MKWKGRTASTNVEDRRGTAKKGFIGGGAGMILVVLVTLLLGGDP
                     GSIISNLQDSNTSGSSAYVETQQEKELAEFVSVVLADTENAWNEILLKEGVDYQEPTL
                     VLFTDSVQSACGVAGSSTGPFYCPADYKVYIDLSFYNELRETFKAPGDFAMAYVIAHE
                     VGHHVQTQLGVMEQVQSLRTKLSETEYNEYLVRLELQADYLAGVWAHYAQGKGLLEEG
                     DIEEALNAASAVGDDRIQKQAQGYVVPDSFTHGTSEQRMRWFKKGYTNGSLAEWDTFH
                     ADVL"
     gene            3836..3964
                     /locus_tag="P5549_08370"
     CDS             3836..3964
                     /locus_tag="P5549_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13126.1"
                     /translation="MEIGIGQKALDFSIADWQGNEFVLSSYLGQKNLFLVFNRGFV"
     gene            3980..4294
                     /locus_tag="P5549_08375"
                     /pseudo
     CDS             3980..4294
                     /locus_tag="P5549_08375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(4371..5903)
                     /gene="guaA"
                     /locus_tag="P5549_08380"
     CDS             complement(4371..5903)
                     /gene="guaA"
                     /locus_tag="P5549_08380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRW13127.1"
                     /translation="MEKELVLVLDFGGQYNQLIARRVRELSVFCEMVPYDISFAEVKA
                     RNPQGIILTGGPASVHIEDAPRCDPRIFSLGVPVLGICYGMQLLAHELGGVVKPSQLR
                     EYGRTSLTVSQPGQLFKDISSPTQVWMSHGDSIDSVPEGCQVNAVTGNCPIAAFSDSV
                     RQLYGVQFHPEVRHTLEGIQVLRNFLFEICALKGDWDLSDFISEKIEEIRAQVGGKKV
                     LCGLSGGVDSSVAAALVHKAVQDQLVCVYVDTGLMRKGESEQVLDTFGRHMHMNLVFV
                     DAGERFLGKLAGVSDPEKKRKIIGEEFIRVFEEEAKKLGKIDYLVQGTIYPDVVESGT
                     KTAHTIKSHHNVGGLPEDMQFALVEPLRLLFKDEVREIGAKLGLDDEIVWRQPFPGPG
                     LGVRVLEEVTSEKVKILQEADHILREEIKKAGLEREIWQYFAVLPPMRSVGVMGDGRT
                     YAYPIILRAVTSDDAMTAEWAHIPYEVLDIIARRIVNEVAGVNRVAYDITSKPPGTIE
                     WE"
     gene            complement(5914..6453)
                     /gene="hpt"
                     /locus_tag="P5549_08385"
     CDS             complement(5914..6453)
                     /gene="hpt"
                     /locus_tag="P5549_08385"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRW13128.1"
                     /translation="MNLDQVKVLYSETEIKDRINQMGAVISRDYQGEELLIIGILKGA
                     FMFMADLVRRIELPLRLDFMDVSSYGSSTISSGEVRIMKDLDYSIEGQNVLIVEDIVD
                     TGLTLNYLCEILRKRNPKTLRIACMLDKPSRRRTNTIPDYIGFSVADEFIVGYGLDYS
                     EYYREYPAICILNPSVYEK"
     gene            6648..7046
                     /locus_tag="P5549_08390"
     CDS             6648..7046
                     /locus_tag="P5549_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRW13129.1"
                     /translation="MKIETLDLHGLNQSEAKAKIDQNLAWIIRHGIDVLVISHGKGHH
                     SSTNFAVLKAETRKLLKANRDLEEAGYLVVYGEADLPIALRYDEGNTLVVARGREQEY
                     MGGSIQQEKDRRIFSEDGRRERKNSKRMRK"
     gene            7208..7507
                     /locus_tag="P5549_08395"
     CDS             7208..7507
                     /locus_tag="P5549_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13130.1"
                     /translation="MAELNDFEIRYTVTVDYKGEVKDGKPNGKGKATFRDGGMYEGDW
                     LNGKQDGYGKEYYKDGILRYEGEYKNGLRHGTGTVYYKDGSKGHSGKWKFNEPAK"
     gene            complement(7636..8232)
                     /gene="cobC"
                     /locus_tag="P5549_08400"
     CDS             complement(7636..8232)
                     /gene="cobC"
                     /locus_tag="P5549_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HRW13131.1"
                     /translation="MKILLLRHGQTEWNALKKYQGHMDIALNDAGRAQAEKLARYLRC
                     NEKIEAIYCSDLSRSRETAEIIARELQLSIQVDERFREIGFGDWEGMTYEEVSRMYPE
                     EYTKWFNNNLTIKVPGGESIDDLLARTLPSLGDLAQRHAGTVLVVSHGGLIKTLLNHL
                     MGSERWDTYLYPASISSLEWDGEEFVPQLIGFTLPDEV"
     gene            complement(8268..8831)
                     /locus_tag="P5549_08405"
     CDS             complement(8268..8831)
                     /locus_tag="P5549_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRW13132.1"
                     /translation="MIQIYTGDGKGKTTAALGLAMRSVGHGYRVRMIQFLKGSTYSGE
                     LTSAARLGIEVYQFGRTCAHAAVIKSGFMTCQECGECWVDVDNINELDRQKTRMAWEL
                     VQATMAQGQHDMLILDEIFHAVNLQLVSRDELSVLLRTVPASLEIVLTGRNADAQWIT
                     MADLVTEMKMIKHPYAQNCKARRGIEY"
     gene            9079..9181
                     /locus_tag="P5549_08410"
                     /pseudo
     CDS             9079..9181
                     /locus_tag="P5549_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
BASE COUNT         2316 a         2420 c         2180 g         2265 t
ORIGIN      
        1 ccaccgggca ggtcgcggag atctacgatg aaggcttcat tgccaaacgc atggaagtag
       61 gtgcggtgat cggcgccgga ccgaaaagca aggtcgttcg ggccactcct gcgccgggcg
      121 atatggtcat ccttctgggc ggcagaaccg gccgggacgg ctgcggaggc gctaccggct
      181 cctcggtgga acataccgaa gaatccatct acaccagcgg cgccgaagtg cagaagggca
      241 acgcccccac cgaaagaaag attcagcgat tgttccgtaa cccggaggcc agccggctga
      301 ttaaaaaatg caatgatttt ggagcgggcg gagtctccgt ggccattggc gaattgaccg
      361 acggactgct cattcaattg gacgctattc ccaagaaata cgacggtctg gacggcactg
      421 aactggccat ttccgaatcc caggaaagaa tggcagtagt ggtcgcccgg gaacattccg
      481 ggcggtttaa agaactggca gatatggaaa acctggaagc caccgaggtg gcggaagtaa
      541 aggcagaccg acggctgaag atggtttggc gcggccagac catcgtggac atcgcccgcg
      601 actttcttga caccaacggt gtcaaacagc atatcgaagt ccaggttgcg gaaccggatg
      661 cggataaaaa ctattttaaa aacaaaccgg caactatctc tgccgatctt aaaacagcct
      721 ggctgaataa cctacaggat ctaaatgtct gctcgcagcg cggtctggta gagtgttttg
      781 acagcaccat tggcgccgga accctcctga tgccttttgg cggccattat caagctactc
      841 ccggcatcgg catggccgcc aaacttccgg tggaagaagg cgacaccact acctgtacgc
      901 tcatgaccta cggttacaac gcccaactca gcaagtggag ccctttccac ggcgctcttt
      961 atgcggtggt ggaagccgtc tccaagatcg tggccatggg cggagactat aaaacagtgc
     1021 gcctgagcct gcaggaatac ttcgaaaaac ttggcaccga ccgctgtaaa tggggcaaac
     1081 cttttgccgc tttgctgggt gcctaccaag cccagatgca gctgggcata cctgccattg
     1141 gcggcaagga cagcatgtcc ggcactttta tggagctcca tgtgccgccc actctggtag
     1201 cctttgccgt caccgccgcc gatacgagga atttgatctc gccggaattt aaacacccgg
     1261 acagccaagt ggtatggatt ccaatcgccc gtgacgccaa tgaattgcct gattttgaag
     1321 cgctgcgcac tgcttatgaa cgcgtcaccc agttgatcca caccggccaa atcactgctg
     1381 cccatccggt acgcggaggc ggtatagcgg aagtcatcag caaaatggcc tttggcaacc
     1441 gcatcgggtt tgccgcacaa gaccatcttt gggcagagga ggaagcaaat gcccgctggt
     1501 ttacgcctga atatggaagt ctggtggtgg agatgcctgc agaggtaaat gttgctgccg
     1561 cgctggatgg tgttacctgc caccacttgg gccggaccat ccaagacgaa atgattgtca
     1621 tcggtcgcat gagcctatcc ctgcaggaaa cccagaccgt atgggaagca ccgttggaaa
     1681 acgtgttccc gacccaaact gaagagctca cactgaccgc ccctcgggtt gtatctcatg
     1741 cgcagcgcgg acgaaccaag gccagtctga cagttgccga accaagggta ttgatcccgg
     1801 ctttcccggg caccaactgt gaatatgaca cccggaagcg atttgagctg gccggtgctc
     1861 aggcggatat ttttgtgata aaaaatctca ccccggcagc cgtggaagac tccatccggg
     1921 atatggcagc gatgatcagg caatcacaga ttatcgccat ccccggcggc ttcagcgccg
     1981 gcgacgagcc ggatggttcc ggcaaattta tcgccgcctt cttccgtagc ccgcgcatcc
     2041 gtgatgaggt gatggaattg ctgaaaaacc gggacggtct gatgctgggc atttgcaacg
     2101 ggttccaagc tctgatcaag ctggggctga ttccctacgg tgagatccgg gacatggacg
     2161 aaacgcagcc cactctgacc tacaatcgta tcggccgcca catgtcacgc atggtccaaa
     2221 cccgaatcgc ctcggtgttg tcaccttggt tggccaacgt ggaggtcggt gacattcacc
     2281 aaatcgcggt ctcccatggc gaagggcgct ttgtcgccag tatagaggag cttgagcaga
     2341 tgatcgccaa tggacagatt gccgcccagt atgtagacga tgacggccag ccctcctatg
     2401 atattcgtta taatcccaac ggctcggact atgcggttga ggccatcacc agtcccgacg
     2461 gacgggtttt gggcaaaatg ggccactcgg aaagaatcgg cacgcacctg tatcgcaaca
     2521 ttcccttgga aaaagaccag cgtctattcg aagccggtgt caattacttt aaatgacagt
     2581 tctgttataa cagcacagaa aaaacttcag gcggctgctt ttaataaagc aaccgcctgt
     2641 tcatcttgtt accttcttat ttataaaaca tcagcatgga aggtatccca ctcggccaag
     2701 ctcccattag tatagccttt tttgaaccag cgcatcctct gctccgaggt cccgtgggta
     2761 aaactatccg gcacaacata tccctgcgcc tgtttttgga tgcggtcatc accgactgcg
     2821 ctggctgcat ttaaagcttc ttcaatatcc ccttcttcca gcagtccctt gccctgggca
     2881 taatgtgccc agacccccgc caagtaatct gcctgcagtt ccagccttac taaatattca
     2941 ttgtattcag tttcgcttag tttagtgcgc agggattgga cctgttccat tacacctaat
     3001 tgcgtctgca catggtgccc gacttcatga gcgatcacat aagccatggc aaaatcaccg
     3061 ggcgctttaa aggtttcacg cagttcattg taaaaactca aatcaatata cactttgtag
     3121 tcagccgggc aataaaacgg gccggttgac gaaccggcaa ctccgcaggc agactgtaca
     3181 ctgtctgtaa ataagaccag cgtaggctcc tgataatcca cgccttcctt taggagaatc
     3241 tcgttccagg cattctctgt atcagccaac accaccgaca cgaattcggc cagctctttt
     3301 tcttgttgtg tttcgacata agccgaactg ccagaggtgt tgctatcctg cagattacta
     3361 atgatgcttc ccggatctcc tcccagcagg agggtgacca gcactaccag aatcattccg
     3421 gctcctccgc cgataaaccc ttttttggct gtgcctctgc ggtcttcaac attggtgctt
     3481 gcggttcttc ccttccattt cataacatgt actcctccaa tttcaggata taatgctttt
     3541 cctcttgctt aactaaggaa ggatttttat ataatatcga atttaatata aatatagcgt
     3601 aatcacagta attataaatg ataatgcaaa agcatgccac cctggtagat ttaaattatt
     3661 aattaaccca gctctacacc aaaaagccca gcaacgatac gatccgaaca gaagctgcgc
     3721 tcctgtcctg gctccaaaag agagggatca tccgagagat tttagctaaa gcatggtaca
     3781 ccgtccatta tatgtgtatt atataatcat acaccatcaa atagggaggg gtaccatgga
     3841 aattggcatt ggacaaaagg ctctggattt ttccattgcc gactggcaag gaaacgaatt
     3901 cgtcttatct agttatttgg ggcaaaagaa cctttttctt gtcttcaacc gcggctttgt
     3961 ctgaccgtac tgccgagcgc atatggcgca gttgcgccaa gactattccc tgtttgaaga
     4021 aaagggaacg gaggttgtag ccgttggtcc ggaagacaaa aagagttttg caaaatactg
     4081 ggatgaaaat aacatgcctt ttcctggaat cccggatccg gaacatcgga tcgccgattt
     4141 gtacaaacaa aaggtgaaca tcttgaaact cggccggatg ccagcccaag tgatcatcga
     4201 caaacaaggt attgtccgtc atatccacta cggaagctcg atgaaggaca tcccggaaaa
     4261 cgaggagatc ctaaggcttc tggatgaact ttaaaaaaat accctaaatc ggtgtatgac
     4321 cggattcagg gtattttcat gtatttcttc cttggttgat tgtggtacat ttactcccat
     4381 tcaatcgttc cggggggttt gctggtgatg tcataggcaa cgcggttgac gcccgctact
     4441 tcgttgacaa tgcggcgggc tataatatcc agcacttcat agggaatatg ggcccactcg
     4501 gcggtcatgg cgtcatcact ggtcactgcc cgcaggataa tcggatacgc ataggtacgt
     4561 ccgtcgccca tcacacccac gctgcgcatc ggcggcagca ccgcaaaata ctgccatatc
     4621 tcccgctcca gcccggcctt tttgatttct tcccgtaaaa tgtgatcggc ttcctgtaaa
     4681 atttttacct tttccgaggt gacttcctcc agcaccctga cccccagacc cggcccgggg
     4741 aacggctgac gccatacaat ctcgtcatcc agaccaagct tggctccgat ctccctgact
     4801 tcatccttga acagcaggcg cagcggttcc accagtgcaa actgcatgtc ctcaggcaat
     4861 ccgcccacat tatgatggct tttgatggta tgggccgttt tggtgccgct ctctaccacg
     4921 tcaggataaa tggtaccttg caccaaataa tcgatcttgc ccaatttttt ggcttcttct
     4981 tcaaataccc ggataaattc ttcaccgatg attttgcgct tcttttccgg gtcgctgacg
     5041 ccggccaatt tgcccaggaa tctttcgccg gcatcgacaa acaccagatt catatgcata
     5101 tgccggccaa atgtgtcgag tacctgctcg gattctcctt tgcgcattaa ccctgtgtct
     5161 acgtagacgc aaaccagttg atcctgcaca gccttatgca ccagagcagc agccacggag
     5221 gaatcaaccc cgccgctcag cccgcaaagc acttttttac cgcccacttg ggcgcggatc
     5281 tcctcgatct tttcactgat aaaatcactg aggtcccagt ccccttttag tgcgcagatc
     5341 tcaaaaagga agttgcgcag gacctgtatg ccttccaagg tatgtctgac ttcgggatga
     5401 aactgtacac catataattg gcgaacactg tcgctaaaag cagcaatggg acaattaccg
     5461 gtgactgcat taacctgaca gccttccgga acgctgtcaa tggagtcgcc atgactcatc
     5521 cacacttggg tgggagaact aatgtcttta aacaattgac ccggttggct taccgtcagg
     5581 ctggtacgcc cgtactcccg caactggctt ggtttgacga cgccacccaa ttcatgggcc
     5641 aacaattgca tgccataaca aattcccaac acgggcacac ccaaagaaaa aatccgcgga
     5701 tcacagcggg gcgcatcctc aatatgtacg ctggccggtc cgccggtcag aataatcccc
     5761 tggggattcc tggccttcac ctcggcaaag ctgatatcat aaggcaccat ttcgcaaaaa
     5821 acggataact ctctcacccg tcgggcaatc aattgattat actgaccacc aaaatccaga
     5881 accagcacga gttctttttc catcattaca cctctacttt tcataaactg acgggtttaa
     5941 aatgcaaatt gccggatact cgcgatagta ttcggaataa tccagtccat accctacaat
     6001 aaactcatcg gcaacagaga atcctatgta atcagggatg gtattggtcc ggcgccgcga
     6061 aggcttgtcc aacatacagg caatccttaa ggttttggga tttcttttgc gcagaatttc
     6121 gcaaaggtag ttcagagtca agccggtatc gacgatatcc tccacgatta aaacgttttg
     6181 cccttcaatg gaataatcca gatctttcat gatccgcacc tctcccgaag aaatggttga
     6241 agatccgtaa ctggatacat ccataaaatc caaacgcaaa ggcaattcga ttcgcctgac
     6301 caggtcggcc ataaacataa aggccccttt taatataccg atgatcagca attcttctcc
     6361 ttgatagtct ctgctaatga ccgcccccat ctggttgatc cggtctttaa tttctgtttc
     6421 gctatacaat actttgacct ggtccagatt catctgccaa ttcctcctta tataaaagga
     6481 ttataacaac aaccctgttg cggcgcaatt gatgacagaa atttccctgc caactttgca
     6541 tccacaagca cacctgtgtt acatttctat tgagttgttg tatcttcatg gtgaaccggc
     6601 aggcaggagg ccgggccgtt gatattctct ataagggagt ggttgtgttg aaaatcgaaa
     6661 cgcttgactt gcatggccta aatcaatctg aagccaaagc caaaatagat caaaacctgg
     6721 cctggataat ccgtcacgga atcgatgtcc tggtcatcag tcacggtaag gggcatcaca
     6781 gcagcaccaa ttttgcagta ctgaaagccg agactcgcaa gcttttaaaa gccaaccgtg
     6841 atttggagga agcagggtat ctggtcgtat acggagaagc agacctgccg attgcgctgc
     6901 gttatgatga aggcaatacc ttggtggtag cccggggaag ggagcaagag tatatggggg
     6961 gcagcattca gcaagaaaag gaccggcgaa ttttttccga ggatgggcgc cgggagcgga
     7021 aaaacagcaa acggatgcgc aaatagtatt ggcagcatca gtctttcgca ttgttgtctg
     7081 ggtgatgtgt aaattttatg cattttattc catattttga tattcaaatc aatcgtttac
     7141 atgttatgat taagttagat aaaaagtatt ttggttgatc caatgaaaca ggggaggggg
     7201 agtttggatg gcagaattaa atgatttcga aattcgctat acggtaacgg tcgattacaa
     7261 aggggaagta aaagacggca aacccaacgg caaaggcaag gctaccttcc gggacggcgg
     7321 tatgtatgaa ggcgattggt taaacggcaa acaggacggc tatggcaaag agtattacaa
     7381 agacggcatc ctgcgttacg aaggcgaata taaaaatggg ctgcggcacg gaaccggcac
     7441 agtatattac aaagacggga gcaaaggcca cagcggcaag tggaagttta acgaaccagc
     7501 caaataaaac gttatttttt ggggaaattt gtaaagtccg ttgtatcgtc aagtacagcg
     7561 gactttcatt gttaaacacc aatgttcccg ggacacgcca gcaagctttg ctcgtgtcag
     7621 caggctaggt tcctattaga cctcgtcagg cagggtgaaa ccgatcaact gcgggacaaa
     7681 ttcctctcca tcccattcaa ggctggagat ggaagccgga tagagataag tatcccagcg
     7741 ctcgctgccc atgagatgat tgaggagcgt cttgatcagt ccgccgtgac taaccaccag
     7801 caccgtgcct gcatgtcttt gggccaagtc acccaatgac ggcagcgttc tggccagcaa
     7861 atcatcaatc gattctcccc ccggcacttt tatggtgaga ttattattga accattttgt
     7921 atattcctcg ggatacattc ggctgacttc ttcatatgtc attccttccc aatcaccaaa
     7981 gccgatttcc cggaagcgtt catcgacctg tatcgacaac tgcaattccc gcgcgatgat
     8041 ttccgctgtt tcccgactgc gggacaaatc actgcagtaa atagcctcta tcttttcgtt
     8101 gcagcgcaga taacgggcca gtttttctgc ctgtgcccgg cccgcgtcat tcagtgcaat
     8161 atccatatgc ccctgatact ttttcaaggc attccattca gtctgcccgt gtctcagcaa
     8221 caagattttc attacgcttc aaatccctct ttcgccgctt gcttcattca gtattcgatg
     8281 ccccgccgcg ctttacagtt ctgcgcgtag ggatgcttga tcattttcat ctccgtgacc
     8341 agatctgcca tggttatcca ttgtgcatct gcattgcgtc cggtcaagac gatttccagc
     8401 gaagccggaa ctgtccgcag caatacgctc aactcatcgc gggaaaccaa ctgaagattg
     8461 actgcgtgaa agatctcatc caggattagc atatcatgct ggccctgagc cattgttgcc
     8521 tggaccaact cccaggccat gcgggtcttc tgtctgtcca gttcattgat attgtcgacg
     8581 tctacccagc attccccgca ctcctgacag gtcatgaaac cacttttgat aaccgctgca
     8641 tgagcacagg tacggccaaa ttgatacact tcgatgccca accgggcggc cgaggtcagt
     8701 tcaccgctat aagtgctgcc tttcaggaac tgaatcatcc gcactcgata gccgtgcccg
     8761 accgatctca tggccagtcc cagggcagcg gtggttttgc ctttgccgtc tccggtataa
     8821 atctggatca tcctcagccc tcctgtcttt agccaattat accttaattt cgtccgcttt
     8881 taatctgtgt attggcattt tgtctgcggc tggctgttat cttgcttaca ttatttcact
     8941 gtattgtcaa tggttatatt taccgctgga aacttcggtt tatttcagtt aataatggaa
     9001 aaaaggaggc tgtctgttat gaaaaaattc ttgtcatgcg ttataattat ttcttttctg
     9061 tttctacctc tttattatct gaaatccaaa gatatggcgg aaaacaatta ttttagccat
     9121 acttcccccg cttacggcag tccctttgat atgatgcaaa gcctgggagt cagttattct
     9181 g
//
LOCUS       DAOTHZ010000074         8809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_9508_length_8809_cov_6.534499, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000074 DAOTHZ010000000
VERSION     DAOTHZ010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8809
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9508_length_8809_cov_6.534499"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..356
                     /gene="rpsI"
                     /locus_tag="P5549_08415"
     CDS             9..356
                     /gene="rpsI"
                     /locus_tag="P5549_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRW13133.1"
                     /translation="MARVRLVPGSGNININSRSFEDFFPNKTSRLIVLQPMELTNTTG
                     RFDVLCRVNGGGVTGQAGAVRLGIARALIKANPELRPTLKRAGFLTRDPRMVERKKYG
                     LHKARKAPQYSKR"
     gene            494..1291
                     /locus_tag="P5549_08420"
     CDS             494..1291
                     /locus_tag="P5549_08420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRW13134.1"
                     /translation="MFQKFKEMNQPELQYKQAEGRILLIRKKNVLILAMVIFVLLMAI
                     VLAGNKTKLELPAMSYVIAGQVIAVDAGHGGIDPGAIAKDKQEEKDITLAISKKLVRY
                     LNQAGAQAVNLRTGSGDLCGPNFQGSIRERKRHDLALRVEKAQKAKADLYISIHTNAD
                     LSPRWSGAQTFYKAGQQQSEITAIAIQDELTRILGNTNRKAKTGSYYILDKAGMPIVI
                     VEVGFLSNPREARLLSADEYQEKLAFAIFCGIVKSYLPEEKSSNKLE"
     gene            1328..2719
                     /gene="amrA"
                     /locus_tag="P5549_08425"
     CDS             1328..2719
                     /gene="amrA"
                     /locus_tag="P5549_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRW13135.1"
                     /translation="MICYGALMPHPPVMIKAIGGARSQQLQTSLETLSLVSRELLASA
                     PDCIVFLTPHGNVFRDGLSYLVTPRLEGDLADFGWYDRTNLPNDLQLMQAIAERAEAQ
                     RLPLLAIDEPLAERYNLKMHLDHGILVPLYFLQQAGMQEIPILAISVGLLSNEALYKF
                     GQIIQQASDQLGRSVAVLASGDMSHALKEEGPYRYHPDGPRFDQMVRRAFQQGDFADI
                     LRIPPQLRENARECGYPSILILIGAMDGYQPEKIRSSYEGPYGVGYLSVGFKPGPRTE
                     SLLSDLERVHTEQQTALRAQEGPLVRWARLNLEHAVTGRGAPELAAELQELKNQRAAA
                     FVSIKQDGRLRGCIGTILPAYKNLAEEIAHNALAAGLRDPRFPAVTAAELETLEYSVD
                     VLGTPESCSREELDPHRYGVIVSDKGRRGLLLPDLEGVDTVDEQLHIALQKAGIAPHE
                     PYNTERFEVIRHK"
     gene            2798..3811
                     /gene="amrS"
                     /locus_tag="P5549_08430"
     CDS             2798..3811
                     /gene="amrS"
                     /locus_tag="P5549_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRW13136.1"
                     /translation="MTDKPVRYYSLEDNGYKRCLVCPHHCRLKEGQRGICRVRMNQEG
                     QLLATNYGEFSALALDPIEKKPLYHFYPGSLILSAGSFGCNLSCPFCQNYHIAHQMPA
                     TRYISPDELVEINLACWAQGSIGMAFTYNEPVMWLEYIMDAAPLLQEQGFKVVMVSNG
                     YIETRPLQDLLGWVDAFNIDLKGFSERYYQRMCMGKLAAVQETLTTIAARAHLEVTNL
                     LIPGENDREDEIRDMCCWLAKLDPEMVLHFSAYHPAYKMNLPPTSGETLRRAADIAHE
                     YLKHVYLGNLPGEGNDSLCPDCGQVLISRTGYKVEKPGLEQGRCKNCGKDIPFLIEFA
                     ARP"
     gene            complement(4171..6003)
                     /locus_tag="P5549_08435"
     CDS             complement(4171..6003)
                     /locus_tag="P5549_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW13137.1"
                     /translation="MAQNFMWDTRDHKFILKEWLDMDKIFGFDRFNGVYSKDDIDAIL
                     AEAVKVAKDVIAPSQDDNDAVGAVFKDGKVTVPESFKKAYWFVQENGWGSCNADHHYE
                     GALPKTLYTAVAEALASANAGLAPYYMATAGSAELIQDFGNDHLKELFLPKMFSGEWA
                     GTMDLTEPNGGSDVGDLLTRAFPTDTPGLYKIRGSKCFITGGEQDITENIIHLTLARV
                     EGARPGTSGISLFVVPKFWVNEDGSLGKFNDLNCIGIEHKMGIKGSATASLAFGEENE
                     CYGWILGDPPGEDGKGQGMAQMFNMMNTERATTGTAALAESTVAYHNAVQYATERIQG
                     RPLTNPKAGRVQIIKHEDVRRMLLDIKAHLDAMRALSMKTYYYMDIIINSKDPEEVKQ
                     ADWRYGISTPLCKAYLSDMAWILTAEAMQVYGGYGYSEENPIAQLARDVKIYSIWEGT
                     NYIQSMDLVGRKWMQAKGQAFNDFLGDIFAFLKANADNQEFARELEILNKAIRSYNEI
                     RKAIQEHAMGGKIGFIGLYATRILHATAKLYCGYLLLDQALLASQRIKELGEDHFDYA
                     FYKGKVESAKYYLRNIVPEIFTVTELIKVGDSSAIDIPEESFQF"
     gene            6322..7359
                     /gene="argC"
                     /locus_tag="P5549_08440"
     CDS             6322..7359
                     /gene="argC"
                     /locus_tag="P5549_08440"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRW13138.1"
                     /translation="MFKVAIIGDGYTAAELLRLMAGHDQMEATMVTSTDNIGININRL
                     YPPLRACYDLVCQGTDLEQIKRQCQAVFLALPHGLSVPLVRELTEAGLPCVDLGADFR
                     LQDVNIYEKHYEIRHEAPELLNEAVYGLPELYRKKITAARIIANPGCFPTGAIVPLVP
                     LLETNLIEPRGIVIDSKTGVSGAGKGMKPSSHFCEVNEGIKAYGVGTHRHAPEIVQEL
                     SRAAGQKVGMIFTPHLVPMNRGILTTSYAQMRPGVSARQIREALEDKYQSELFVQILE
                     PGVFPHTKWVYGSNYIYIGVYADEESGQVVLVSALDNLCKGASGQAIQNMNLMLGIEE
                     AKGLLGMGVHP"
     gene            7509..8714
                     /gene="argJ"
                     /locus_tag="P5549_08445"
     CDS             7509..8714
                     /gene="argJ"
                     /locus_tag="P5549_08445"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRW13139.1"
                     /translation="MKVLAGSVTAPQGFLAQGVYAGIKKDDKNDLALIYSEYPCRAAG
                     VFTTNVVRAACIDFNRRQLQDGQARAIVANSGNANACTGEQGGEDTLAMAALTAECLG
                     IEPREVLVASTGVIGVNMPMEQVEAGIIAAANTLSHAGDANAALAIMTTDLVNKEIAV
                     EITIGGKAVRIGAMAKGSGMIHPNMATMLGFITTDAAISSNCLQIMLTDVVEKSFNMI
                     SVDGDTSTNDMVLILANGQAGNPVIEDPSSQEGLTFYQALEYMAVYLAKMIARDGEGA
                     NHLIEVKVNHAADEETARTIARSITSSSLTKAAVFGLDANWGRILAAAGYSGAIFDPG
                     RVDIYLGQEMVARDGMGLTFDEEKARRELEKDTVTILVDLKAGEASATAWGCDLSYEY
                     VRINAAYRS"
BASE COUNT         2328 a         2019 c         2331 g         2131 t
ORIGIN      
        1 aaacagcaat tgccagagtt agattagtcc ctggcagcgg aaacataaat atcaacagcc
       61 gcagctttga agatttcttt cccaacaaga ccagccgtct gatcgttttg cagccgatgg
      121 aactcactaa tacgaccgga agatttgatg ttctttgccg ggtcaacggc ggaggtgtta
      181 ccggacaggc cggcgctgtc agactgggga ttgcccgggc gctgatcaaa gccaacccgg
      241 agctcagacc cacattaaaa agagctggtt tcctgactcg cgatccgcgt atggtggaga
      301 gaaagaaata tggtctgcac aaggcccgca aggctccgca atacagcaaa cgttaatgat
      361 gtagatgata aaacaccttc tggagtattc cagggggtgt ttttatttgt ggagagtctg
      421 gtttaacgtt cttatattat tttcctgctg cgcatataaa tagatatccg aaatgccaac
      481 aggagggata cagttgtttc aaaaattcaa ggaaatgaat cagccagagc ttcaatataa
      541 acaggccgaa ggcaggatct tgttgatacg aaagaaaaac gtccttatat tggcaatggt
      601 catttttgtt ttgctcatgg caattgtgct ggcggggaat aagacaaagc tggaactgcc
      661 ggctatgtct tatgtgatcg ccgggcaggt gatagctgtt gatgccgggc acgggggaat
      721 tgatcccggc gccattgcca aggacaaaca ggaagaaaag gacattaccc tggctattag
      781 taaaaagttg gtccgttatt taaaccaggc cggtgcccaa gcggtcaatc tgcgaaccgg
      841 cagtggagat ttgtgtggac ccaactttca gggcagcatc cgggaaagaa agcggcatga
      901 tttggccctg cgggttgaga aagcacaaaa ggccaaggcc gatctgtata taagcattca
      961 taccaatgcc gacctcagcc ctcgctggtc cggtgctcaa actttttata aagctggtca
     1021 gcaacaaagt gaaatcacgg cgattgccat tcaggatgaa ctgactcgca ttttggggaa
     1081 tactaatcgt aaagccaaaa ccggcagcta ttatattctg gataaagctg gcatgccgat
     1141 tgttattgtt gaagttggat ttctgtccaa tccccgcgaa gcaagattgc tgtctgcgga
     1201 tgaatatcag gaaaaattgg ccttcgccat attttgcgga attgtaaaat catacttgcc
     1261 agaagaaaaa agcagtaata agctggaata aaagaaaaac aacaggaaac aacaaggagg
     1321 caggaccatg atctgttatg gagccctgat gccgcatccg cctgttatga ttaaagcaat
     1381 tggaggtgcc cggtcgcaac agctgcaaac cagcctggag acactgtcgc tcgtaagcag
     1441 ggaactgctg gcgtcagcac cggattgcat cgtatttctg actccccatg gcaatgtttt
     1501 tcgggatggt cttagttacc ttgtcacacc ccgactggaa ggcgatttgg ctgattttgg
     1561 ctggtatgat aggacaaacc tgcccaatga tttgcagctg atgcaggcca tcgctgaacg
     1621 ggcggaggcc caaagactgc ctttattggc aattgatgaa ccactggcgg aacgctataa
     1681 cctgaagatg catctcgacc atggtatttt agtgccactc tactttctgc agcaagcggg
     1741 tatgcaggaa ataccgattt tggctatcag tgtgggattg ttgtccaatg aagcgttata
     1801 caaattcggc cagatcattc agcaagccag tgatcagttg gggcgcagcg ttgcggttct
     1861 ggcatccgga gacatgtcac atgccttgaa agaagaagga ccttatcgct accatcccga
     1921 cggtccccgc tttgatcaga tggtaagacg agcttttcag cagggagatt ttgcagacat
     1981 cctcagaatc ccgccacagc tgcgggaaaa tgcgcgtgaa tgcgggtacc cttccatcct
     2041 gattctgatc ggcgcaatgg atggatacca gccggaaaaa atcaggagca gttatgaagg
     2101 accttatggc gtgggctatc ttagcgtcgg ctttaaaccc ggccccagga cagagagttt
     2161 gctgtcggac ctggagcgcg tacataccga acaacagacc gctcttcgtg cccaggaggg
     2221 acctttggtg cgctgggcca gattgaatct ggagcatgcc gtgaccggac gcggggcacc
     2281 agagctggcg gcagagctgc aggaactgaa aaaccaaagg gcagccgcat ttgtatccat
     2341 caagcaggac ggaagactga gaggctgtat cggtaccatt ttaccagcat ataagaatct
     2401 ggctgaagaa attgcccata atgcactggc cgcaggactt cgggatcccc gctttccagc
     2461 ggtgaccgcg gctgagctgg aaacgcttga atactcggtg gacgtgctcg gtacaccgga
     2521 aagctgttcc cgcgaagaat tggatcctca tcgttatggg gtcatagtta gtgataaagg
     2581 gagacgggga ttactgttgc cggatctaga aggggtcgac acggtggatg aacaactgca
     2641 tattgcgctc cagaaggccg ggattgctcc gcacgaacca tacaatactg aacgtttcga
     2701 agtgatacgc cacaaataag gagatccaat cagaaaaatt aatacgcatg ccattgtgat
     2761 ggcaatgcca gggaaatata acggaggtgc tgggatagtg accgacaaac cggtacggta
     2821 ttatagcctt gaagacaacg ggtacaagcg ctgtctggtc tgcccgcatc actgccgtct
     2881 gaaggaagga cagcgcggaa tatgccgggt tcggatgaac caggagggac agttgctggc
     2941 gaccaattat ggtgaattct ccgccttggc ccttgaccct attgaaaaaa agccgctcta
     3001 tcatttttat cctggttccc tcatcctgtc agcaggaagc tttggctgca acctgagctg
     3061 tcccttctgc cagaattacc atatcgctca ccagatgccg gccacccgtt atataagccc
     3121 ggatgaattg gtggagatca atctggcttg ttgggcgcaa ggttcgatag gcatggcttt
     3181 tacgtacaat gaaccggtca tgtggctgga atatataatg gatgcggcac cgcttctgca
     3241 ggagcagggc ttcaaggtgg tgatggtcag caatggctat attgaaacca ggcctttgca
     3301 agatttgctt ggctgggtgg atgctttcaa catagattta aaaggtttta gtgaacggta
     3361 ttatcagcgt atgtgcatgg gaaaactggc tgctgttcag gaaacgttga caacgattgc
     3421 cgcccgcgcg catttggaag taaccaatct gctgatccct ggagaaaatg accgcgagga
     3481 tgaaatacgg gacatgtgct gctggctggc caagctggat cctgagatgg ttcttcattt
     3541 ttcggcctat catcccgctt ataaaatgaa tttgccgcct acttcgggtg aaaccctgcg
     3601 gcgcgctgcg gatatcgccc atgaatacct aaaacatgtt tatctgggta acctgcccgg
     3661 agaaggcaac gacagcttat gccccgactg cggacaggta ttgattagcc gcaccggtta
     3721 taaggttgaa aaaccaggtt tggaacaggg caggtgcaag aattgcggga aagacatccc
     3781 cttcctaatc gaatttgctg cccgcccata aaggcaagag ggaggctgcc cggccaattc
     3841 accaatggat tgcggacagc ccccgtcttt tatatgaaag ccccgggacg aatcccgtaa
     3901 gctgttcaac gatgtatggt ttacaatatg ccaaatattt gataaaggtt acatcgcagc
     3961 aacgcaaaca agtgaatggc tgtgctgcag agaaagtcag ttgaatggcg gtacataaca
     4021 atggggacaa tcctctttat gagaattgtc cccattgcta tatggcatca tgcatggtct
     4081 tgccatcggt catgtatttc ctaatcggtg ggattgcatg caatgatcgg ccaggaaccc
     4141 gtttgtttga acgagctcct ggcaattgaa ttagaactgg aaggactctt ccggaatatc
     4201 aatggcagag gagtcaccga ctttgatcag ttccgtcacg gtaaagatct ctggaacgat
     4261 attgcgcaga tagtatttgg ctgactccac tttgcctttg tagaaagcat agtcgaaatg
     4321 atcttcgccg agttccttaa tccgttgact ggccagcaat gcctgatcca gaagcagata
     4381 accgcagtag agcttggcgg tagcatggag gatgcgggtg gcatacagac caataaagcc
     4441 aattttaccg cccatggcat gttcctggat ggctttgcgg atctcattgt aggaacggat
     4501 tgctttattc agaatctcca gctcacgtgc aaattcctga ttgtcggcat tggccttgag
     4561 gaaagcgaaa atgtcaccca ggaaatcatt gaaggcctgt cccttggcct gcatccattt
     4621 gcggccaacc aaatccatgg actggatata gttggtgcct tcccagatgg aatagatttt
     4681 gacatctctg gccaattggg caatcggatt ttcttcggaa tatccgtagc cgccgtaaac
     4741 ctgcatggct tccgcagtca agatccaggc catatcggaa agataagcct tgcaaagcgg
     4801 agtgcttata ccgtagcgcc aatcggcctg tttgacttct tccgggtcct tggagttgat
     4861 gatgatatcc atatagtaat acgttttcat ggagagagcc cgcatggcat ccagatgtgc
     4921 ttttatatcc agcagcattc tgcgaacgtc ttcatgttta atgatctgca cgcgtccagc
     4981 cttggggttg gtcagcggac ggccctggat acgctcggtg gcatattgta ctgcgttgtg
     5041 gtaagcgaca gttgattcag ccagcgcagc ggtcccggtg gtggcccgct cggtattcat
     5101 catgttgaac atctgcgcca tgccctggcc cttgccgtct tcaccgggag gatcgcccag
     5161 aatccagccg taacattcat tctcttcccc gaaagccaga gaagcggtag cggaaccttt
     5221 gatccccatt ttgtgctcaa tgccgatgca gttgagatca ttgaatttcc ccaggctgcc
     5281 atcttcattg acccagaact tcggaactac gaagagagag atgcccgaag tgccgggacg
     5341 tgcgccctca acccgggcca gggtcagatg tataatgttt tcagtaatgt cttgttcgcc
     5401 gccggtgata aagcacttgg aacctctaat tttataaagg cccggagtat cagtcgggaa
     5461 agccctggtg agcagatcgc ccacatccga gccgccgttg ggttccgtca aatccatggt
     5521 gccagcccat tcaccggaaa acattttagg caggaagagt tctttcaggt ggtcattgcc
     5581 gaagtcctgg attaattctg ccgaacctgc agttgccatg taatagggtg ccagaccggc
     5641 attggcagag gccagtgctt ctgcaacggc tgtatagagc gttttgggca gggcaccttc
     5701 ataatgatga tcggcattgc aggaacccca accgttttcc tggacaaacc aataggcttt
     5761 tttaaaactt tcgggaacgg tcacttttcc atccttgaat accgccccta ccgcatcatt
     5821 gtcatcctgg ctgggagcaa tgacgtcttt ggcaacttta accgcttcgg caagaatcgc
     5881 gtctatgtca tctttggagt agacaccgtt aaaacgatca aaaccaaaga ttttatccat
     5941 atcgagccat tccttgagaa taaacttgtg atcccgggta tcccacataa agttttgtgc
     6001 caatttccat acctccaaaa ataaaatgat tgcttggtta cagcgaatcc gaaatgtata
     6061 acgagcagaa attccctttc atgatacccc ctaacttttt ttaagatata atcttgcgca
     6121 cggcgataaa gcatcatcca tgaatagcca aatagttggg tgcaaaatta tatttattaa
     6181 tattatataa cattcgttaa tattgataca gaatatattt acgataacca aatccaaaaa
     6241 agaggaaaaa aatttgcgca aacgattgca aatatatgca gctgattgta taattatata
     6301 atcttgcgag gaggtacaga tatgtttaaa gttgcgatta ttggagatgg ttatactgct
     6361 gccgagctat tgcgattgat ggcgggacat gatcaaatgg aagcgacgat ggtcacctcc
     6421 accgacaaca taggtataaa tatcaacagg ctatatcctc ccttgagagc ttgttatgat
     6481 cttgtctgtc agggcacgga cctggaacag attaaaagac agtgccaggc tgtgtttcta
     6541 gcacttcctc acggattatc agtacccctg gttagagaac tgacagaagc aggtttgccc
     6601 tgtgttgacc tgggcgcgga ttttcgtctt caggatgtga atatatacga aaaacattat
     6661 gaaatcaggc atgaggcgcc ggaattgttg aatgaagcgg tttacggatt gccggaatta
     6721 taccggaaaa aaatcacagc cgcccggatt attgccaacc cgggatgctt tccaaccgga
     6781 gcaattgtac cgctggttcc cctgctggaa acaaatctca ttgaaccccg cggcattgtt
     6841 atagattcaa agaccggcgt atcgggtgcc ggcaagggga tgaaaccaag cagtcatttt
     6901 tgtgaagtga atgaaggtat taaagcgtat ggagtaggaa cccaccggca tgcaccagag
     6961 attgttcaag aactgagccg ggcagcaggc caaaaagtag gcatgatttt tacgccccat
     7021 ctggtgccga tgaatcgagg tattttaacg accagttatg cccagatgcg gccaggtgtc
     7081 tccgcccgtc agatcagaga ggctttggaa gacaaatacc agtccgagtt gtttgtacag
     7141 atcctcgaac cgggcgtatt ccctcacacc aaatgggtgt atggcagcaa ttatatctac
     7201 attggtgtct acgccgatga agaaagcggt caggtggttt tggtttctgc tctggataat
     7261 ttgtgcaagg gggcttccgg tcaggccata caaaacatga acctgatgtt ggggattgaa
     7321 gaagccaaag gattgctcgg gatgggcgta catccctagg gaaatagacg catgcaggag
     7381 aagcttaggg gattaaacaa atgtaaaata tttacaaaaa acaactttga ccccggtata
     7441 gaccggttaa agctgatccg cataaagata gtgaaacaca tttctcgata atgatgagga
     7501 ggaaaatcat gaaggtatta gctggttcgg ttactgcccc gcagggcttc ctggcccagg
     7561 gggtttatgc cggaattaaa aaagatgata aaaacgattt ggccctgatt tattccgaat
     7621 atccctgtcg ggctgccggt gtattcacca ccaatgttgt acgggcagcc tgtatcgatt
     7681 ttaaccggcg ccaactgcag gatggacagg cgcgggcaat cgtggctaac agcggcaatg
     7741 ccaacgcctg caccggtgag caaggcgggg aggataccct ggctatggct gcactgactg
     7801 ctgagtgctt gggaattgaa ccgcgggagg tattggtggc ttcgaccggg gtcattgggg
     7861 taaacatgcc catggagcag gttgaagccg gcatcattgc ggcggccaat acgttgagcc
     7921 atgccggcga tgccaatgct gccctggcca tcatgacgac agatttggtc aacaaggaaa
     7981 ttgccgtaga gattaccatt ggcggaaaag ccgttcgaat aggtgccatg gccaagggtt
     8041 ccggcatgat ccatcccaat atggccacca tgctgggctt tattacaaca gatgctgcca
     8101 tcagcagcaa ttgtttgcaa ataatgctta cagatgtcgt tgaaaaatct tttaatatga
     8161 tcagcgttga cggtgatacc tctaccaatg acatggtttt aattttggcc aatggccagg
     8221 ccggcaaccc cgtgattgaa gacccctcca gccaggaggg actgacattt taccaggcgc
     8281 tggagtatat ggccgtttat ctggccaaga tgattgctcg tgacggcgaa ggggccaatc
     8341 atttgatcga agtcaaggtg aaccatgcgg ctgatgagga aaccgctcgg accatagctc
     8401 gttccatcac ttcttcgagc ttgaccaagg cagcggtttt tggtctggat gccaactggg
     8461 gacgaatact ggcggcggcc ggctattcgg gagccatatt tgacccgggc cgggtggaca
     8521 tttatctggg tcaagagatg gtggcccggg acgggatggg gctgactttt gacgaagaaa
     8581 aagcccgtcg ggaactcgag aaggatacgg tgacaatcct ggttgatcta aaagcaggag
     8641 aagcttcagc gacggcctgg ggctgtgatt tgtcctacga atatgtgcgg atcaacgcag
     8701 cctaccgttc ctaagcagtg acgggcataa ttggaggggg aaagacaatg attttatcag
     8761 caatggaaaa agcggaggtg ctggttgaag ccctgcctta tatcaaaga
//
LOCUS       DAOTHZ010000075         8787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_9561_length_8787_cov_6.426706, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000075 DAOTHZ010000000
VERSION     DAOTHZ010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9561_length_8787_cov_6.426706"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="P5549_08450"
     CDS             <1..197
                     /locus_tag="P5549_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13140.1"
                     /translation="SAMSKCYAADMANRVAANAVQILGGYGFCRDYPVEKMMRDAKIL
                     QIFEGTSQVQRIVIGKSLLA"
     gene            264..1442
                     /locus_tag="P5549_08455"
     CDS             264..1442
                     /locus_tag="P5549_08455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HRW13141.1"
                     /translation="MREVVVVGARRTAIGDYLGSLKDKTAVELGIAALNAAIAKAGIE
                     PAQIEEVAAGQVYQAGCLGNPARQVTLGVGCPVETVALTVNQQCPSSMRCTEIISQEI
                     MLGKINIGAVVGMESMSNIPYLLTKARGGYRMGNGVVYDAMLCDGLIDVFYNYHQGVT
                     AENLAVKYGISRAEQDELALLSHQRACAAIDAGKLTEEIVPIEIKSKKGTVLFDTDEH
                     PNRNTNLEALARLKPAFKENGTVTAGNASAINDAACAAILMGGDKAAELGIKPLARIT
                     ATASASVDPSIMGIGVVPAVKRVLQIAGMKKEDIDVWELNEAFAAQFLACNRELQLDM
                     NKVNLNGSGISLGHPVGNTGLKIIIAVINELRRQKKQFGCASLCAGGGPAMATIVEVL
                     "
     gene            1696..2967
                     /locus_tag="P5549_08460"
     CDS             1696..2967
                     /locus_tag="P5549_08460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRW13142.1"
                     /translation="MTAMTNLPPGSLQGVTVVDFTWVLAGPHCTKLLADMGATVIKIE
                     PYGIGANERHLAIQKTCNGVTQSSYSINVNRGKKSFCVDLKKNQGKELVRALISQADV
                     VVENYAPGVMQRLGLDYEAVKQIKEDIIYCSISCFGHWGPYSQKPGYDMIAQVASGWT
                     AQSEVNQIAPNSIGDTMAGVHAALAVVAAVLVKKEQGIGQNIDIAMMDCLFSLHENSI
                     PWYLLGQAVGEPVHPGKIGRLHPGYAPYGLYQGKNGVVGIALLSDNRWETMVRTMGEK
                     YLWLLDDPRSQNVTTRCQNPQVVNDALDEWVMEQESVEEVERIFDEVGIPCMRARTIE
                     ELCDTDLQIKAREMMTIIDQPFIGPMKMYGSPIKMSATPSCVRGHSPLLGEHNHQVMA
                     ELLGFGQAQIEQFYQAEVLHHEAAVDRFNHL"
     gene            3096..3500
                     /locus_tag="P5549_08465"
     CDS             3096..3500
                     /locus_tag="P5549_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW13143.1"
                     /translation="MNSKELGVKIQMAREEKNMSQEELAQAIGCSQSALSNYEKGKRH
                     IYLEQLERLSAILNKPLDYFVDSLGGETNKAFKENDENDEYKTASIKEYNHEHNKRFI
                     KLMNDIYELSDESFYALRDYVRFLQWKQNKEE"
     gene            3504..4649
                     /locus_tag="P5549_08470"
     CDS             3504..4649
                     /locus_tag="P5549_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13144.1"
                     /translation="MDLSLDTKQLQYINLIKELGEKEIIPYSSKHDHSVDVSFDWQLV
                     KILGEHNLVCPTIPEAYGGLGLDHFTTALIMEEIAAICPGLAAIIDTNVHAVEPLLLA
                     GSEQQKEAFLPALTGKNAGLAAFALTEPTGGSYVNGMSTNAEKQPWGYVINGQKDYVL
                     NAPLAQFILLFAFTDHENKRNSMRAFIVPAQTPGCMIGPGHDLAALDYAQTAAISFEN
                     VNLSNDLIIKEREVCSGYLLMGQTLDLGRALVGATAVGIARAAYDLAREFADTRRQNG
                     VGIRQHQAIAHMLADMATKIELARLMTWKACWLIDQGGDYTVPSAMAKVTASTFAQEV
                     TCMASDIMAARAFEKGSFIEQLVRDARVLSTLEGTNIIQKNLIASLL"
     gene            4668..4850
                     /locus_tag="P5549_08475"
     CDS             4668..4850
                     /locus_tag="P5549_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW13145.1"
                     /translation="MYIILAEKCSNCGLCADVCPVEAISPIGVYRIKAELCISCGLCQ
                     ESCPSGAIVIIDQPRE"
     gene            5062..6309
                     /locus_tag="P5549_08480"
     CDS             5062..6309
                     /locus_tag="P5549_08480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13146.1"
                     /translation="MFEFLMTPEQLKLRDEARDLAKSVTRDYILDMDADKIKYPKEYL
                     RECGRRGLIGLRVPKEYGGRGLKWEDDIIALEEICVNGYMFGCSYGVTGDVVPEAIVN
                     FGTEEQKKKFLPSICKGEKFAAECLTEPRGGSDFFGSTTYARRDGDDFILDGQKRFIV
                     GGEGADYFLVYAKTDPEAKANKSMTCFMVEREMGVKTEYLYGLMGCRGGGTARVVFDN
                     VRVPKENIIGEVNGAYPIFYQMMIPERFGTAAMTIGAVRPALEIATDYTLKRKAFGQT
                     INNFQGVSFKIADSAAKLDSCRSFMYTTARAVDSGADPNLCRRLVSEGKKFVTEQCWD
                     VANLCMQAMGGIGYTDVYPLEKILRDMRLSMIWVGSNEIMNVIVQHEWYTERKKELKN
                     NTTKRASEFDAASAFDEDEKIYE"
     gene            6389..7756
                     /locus_tag="P5549_08485"
     CDS             6389..7756
                     /locus_tag="P5549_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13147.1"
                     /translation="MYEKWQQMYKEKLKTPEEAAQMIINGDKISLPTANGTPRVLWQA
                     IAKRIANGELVDSELTVGLNLNAPYLCTPEIASQIHYRAGYISPFERPLAQKGVIDPW
                     PVRFFDIPRSMMDSDYNAVTMTASPMDENGWFSCAMNCTHSYSLTRRAIKLGKPMKVF
                     LEVNKNAPRVYGHNHFHISEVTAVNEADWDLISLPRAEITQEDVAIASYIAEYIPDGA
                     CVQLGIGGLPDAVGKQLTSKKDLGCHTEMIVDAYLDLYKSGALTNRKKTFMEDKMVGS
                     IIIGQPELYKFVDQNPGIEVHGIDFCANPANIAKHDNFMAINAITECDLGGQCISESM
                     GIVPFSGMGGQADFVQGAWQSKGGKAFLALYSTYTDKKGELHSKISPVVNGWVGITRW
                     DVQYLATEYGCVYLKGTSMRERVQRIVSIAHPDFRDWLVHEAKRLNLIDSASDVNTKQ
                     MRKAI"
     gene            7991..8263
                     /locus_tag="P5549_08490"
     CDS             7991..8263
                     /locus_tag="P5549_08490"
                     /inference="COORDINATES: protein motif:HMM:NF017762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HRW13148.1"
                     /translation="MITKELVERINELASKKKSQGLTFAEQAEQKRLYKIYLASIREQ
                     VKTQLDTIEVVPEGHVCDDTCHHHHHGPGCSHDHHQHGSDGNYKKH"
     gene            8335..>8787
                     /locus_tag="P5549_08495"
     CDS             8335..>8787
                     /locus_tag="P5549_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13149.1"
                     /translation="MIAKRTELNRIILYRSLLEDELVNLVCQMLDCEGAANKVKFEEY
                     FYMISHRLLAYSRYYGTQGDVWQTYFLDLLLQDENPYSLGCECSVDDIDPQLHKMARH
                     DLENLRELYCLNWMNVCKSAFIRLDCNYPSFPGLIPRDPARREARDKLF"
BASE COUNT         2507 a         1837 c         2272 g         2171 t
ORIGIN      
        1 tgtcggcgat gagcaagtgc tatgctgccg atatggccaa ccgggttgct gccaatgctg
       61 ttcagatttt gggggggtac ggcttttgcc gcgactatcc ggtagagaaa atgatgcggg
      121 acgccaaaat actgcagata tttgaaggta cgtcgcaagt gcagcgcatt gtcatcggca
      181 aaagtctatt ggcttaaaca aatgctgaga ttgattacaa agatttgtat tgtttgtatt
      241 gtataaggaa gggtggagag aatatgcgtg aagttgttgt agtgggcgca aggagaacgg
      301 caattggtga ttatctgggg tcgttgaagg ataaaacagc ggttgaactg ggcattgcgg
      361 cattgaacgc ggctatcgcc aaagcgggga ttgagccagc tcaaattgaa gaagtcgctg
      421 cgggacaagt ctatcaggcc ggctgtctgg gcaatcccgc tcgccaggtc acgctgggag
      481 tgggctgccc ggtggaaacc gtagccctta ccgtcaatca gcagtgtccg tcctccatgc
      541 gctgtacaga aatcatcagc caggaaatta tgctggggaa aatcaatatt ggtgcagtcg
      601 taggcatgga aagcatgagc aatattcctt atcttctgac caaagcccgg ggaggttacc
      661 gaatgggtaa cggagtcgtt tatgacgcca tgctttgtga cggattaatt gatgtgttct
      721 acaattacca tcagggggtg accgcggaaa atctagcagt gaagtacggg attagccgcg
      781 cagagcagga tgaactggcg ttgctgagcc atcaacgcgc ctgtgccgcc attgatgccg
      841 gcaaattaac ggaagaaatc gttccgattg aaatcaagag caaaaaagga acggtgctgt
      901 ttgataccga tgaacatccc aatcggaaca ccaacttgga agcactggcc cgcttgaaac
      961 cagccttcaa ggaaaacgga acggttactg ccggcaatgc ttctgccatc aatgatgctg
     1021 cctgcgccgc gattctgatg ggaggtgaca aggcggctga attgggtatc aaacccctgg
     1081 cacgaatcac agctactgcc tcggcttcag tcgatcccag cattatggga attggcgttg
     1141 ttcctgccgt gaaacgggta ttacagattg ccggcatgaa aaaagaggac attgatgtat
     1201 gggaactgaa tgaagccttt gccgcccagt tcctggcctg caaccgcgaa ctgcagctgg
     1261 atatgaataa agtgaatctg aatggttcag gcatttcgct gggtcacccg gtgggcaata
     1321 ccgggctgaa gattatcatc gcggtgatca atgaattacg ccgccagaaa aaacagttcg
     1381 gctgtgcctc cctgtgcgcc ggcggtggtc cggccatggc cacaattgtg gaggttctgt
     1441 aacggttgga ggacagtttt tattggatgt tgcgcaggca agcaggcgaa aaatatttgt
     1501 cgggcgaatc acaaataccc ggaggggttt aatattcggt tcaaatcaag ggatgccact
     1561 ggtgttgctc atacacccag gacatccctt tggtttgccg gcaagagatg aaccggtttc
     1621 gaaaaaaaga attgctgtta taatgataga gaattatata atcatatgtg ttgaaaacat
     1681 gaggagaaga tgaaaatgac agctatgacc aatcttccgc ccggcagtct gcagggcgtg
     1741 acagtagtgg attttacctg ggtattggcc ggaccgcatt gtaccaagct gctggccgat
     1801 atgggggcca cagtgattaa gatagaacct tatggaatcg gggccaacga acggcatctg
     1861 gccatacaga aaacctgtaa cggggttacc cagagttctt attcgatcaa tgtcaatcgt
     1921 ggtaaaaaaa gcttttgtgt ggatttgaaa aaaaaccagg gaaaagaact tgtacgcgcc
     1981 ttgatcagcc aggcagatgt agtggttgag aactatgcgc ccggggttat gcaacggctg
     2041 ggactggatt atgaagcagt caaacagatc aaggaagaca tcatctattg ttctatctcc
     2101 tgcttcggac actggggacc gtacagtcag aaacccggct atgacatgat cgcccaggtc
     2161 gccagcggct ggacggcaca aagcgaagtg aatcagattg ctcccaattc tattggtgat
     2221 acgatggctg gcgtccatgc agcactggcg gtggtcgctg ccgtgttggt gaaaaaagag
     2281 cagggcatcg gacagaacat tgatattgcc atgatggatt gcctgttttc cctacatgaa
     2341 aattcgattc cctggtatct gctgggacaa gccgtaggtg aaccggttca tcccggtaaa
     2401 attggtcgtc ttcatcccgg ttatgcgcct tacggtcttt atcagggcaa aaacggagtg
     2461 gtgggaattg ccttgctgtc cgacaaccgc tgggaaacga tggtcagaac catgggggaa
     2521 aaatatctgt ggctgctgga cgatccccgc tctcaaaacg tgacgacgcg ctgccaaaat
     2581 ccccaggttg tcaatgatgc gctggacgaa tgggtgatgg agcaggaatc ggttgaagaa
     2641 gtggaaagga tttttgatga ggtaggcatt ccctgcatgc gtgccagaac cattgaggaa
     2701 ttatgtgaca ccgatttaca aatcaaagcg cgcgaaatga tgaccataat cgatcaacct
     2761 ttcatcggcc ccatgaagat gtacggcagt ccgatcaaaa tgtcggctac accgtcctgc
     2821 gtacgcggtc acagccccct cctgggggaa cacaatcacc aggtaatggc tgagttgctg
     2881 ggcttcggcc aagcccagat cgaacagttt taccaggcag aggttctcca tcatgaagca
     2941 gcggtggacc gctttaatca tctgtgatta ttttcgacaa tgaaaatcat aacggcaaca
     3001 gaacaagagg gaagggtttt ttcataagag gttatactaa aattacatat gtgataaaat
     3061 cttattataa atacttgcat tagtgagggg caaaaatgaa ttcaaaagaa ctcggggtta
     3121 aaattcaaat ggccagggaa gaaaagaaca tgagccagga agaacttgct caggccatcg
     3181 ggtgttccca atcagcgctt tcaaattacg aaaaaggcaa gcgtcatatc tatttggaac
     3241 agctggaacg gctttctgcc attttaaaca aacctctcga ttactttgtg gacagtttgg
     3301 gcggcgaaac gaacaaagct ttcaaggaaa atgatgagaa tgatgagtat aaaactgcaa
     3361 gtataaaaga atataaccat gaacacaaca aacgttttat taagttgatg aatgatattt
     3421 atgaactgtc cgatgagagt ttttatgctt taagagatta tgtacgattc ttgcaatgga
     3481 agcagaacaa ggaggaataa ggaatggatt tatcgcttga tacgaaacaa ctgcagtaca
     3541 tcaatctgat caaagaattg ggtgagaaag aaattattcc gtattcatca aaacacgatc
     3601 attcagtcga tgtttcattt gactggcaat tagtaaaaat actgggtgaa cataatctcg
     3661 tttgtccaac cattcccgaa gcgtatggcg ggttgggtct ggatcatttt accaccgcgc
     3721 tgattatgga ggagattgca gcgatttgcc caggcttggc ggcgattatt gataccaatg
     3781 tacatgcagt tgaacccctg ttgctggccg gcagtgaaca gcaaaaagaa gccttcctgc
     3841 ccgcacttac cggcaaaaat gccggtctgg cagctttcgc gcttacagag ccaacgggcg
     3901 gatcttatgt caacggcatg tcaaccaatg cggaaaaaca accttggggg tatgtcatca
     3961 atgggcaaaa ggattacgta ttgaatgctc ccctggcaca atttatttta ttatttgctt
     4021 ttactgatca cgaaaacaaa agaaattcca tgcgtgcttt tattgttccg gcccaaacac
     4081 cgggctgtat gattggtccc ggacacgatc tggctgcact tgattatgcc caaacggcag
     4141 ccataagctt tgagaatgta aacttatcaa atgatttgat tataaaagag cgggaagtct
     4201 gcagcggata tttattaatg ggacaaacac tggatctggg aagggccttg gttggcgcta
     4261 cagcagttgg gattgccaga gcggcttatg atttggcccg ggaattcgcg gatacaagaa
     4321 ggcagaatgg agttggtatc agacaacatc aagcgatcgc tcacatgttg gccgatatgg
     4381 caaccaaaat tgaattggcc cgtttaatga cctggaaagc ctgctggctg atcgaccagg
     4441 gaggagacta tacagtacct tcggccatgg ccaaggtaac ggccagtacc tttgcccagg
     4501 aagtgacctg tatggcatct gatataatgg ctgcccgggc ttttgagaag ggctcattta
     4561 ttgaacagct ggtgcgtgat gcccgggttt tatcgacatt ggaagggacc aacatcattc
     4621 aaaagaacct catcgcatcc cttttgtaga attgattggg aggatatatg tatataatat
     4681 tggccgaaaa gtgctctaat tgcggtttgt gcgcagatgt ctgtccagtg gaagccatca
     4741 gtcccatcgg tgtatataga ataaaagctg aattatgcat ttcctgcgga ttatgtcagg
     4801 aaagttgccc ttctggagcc attgtaatta ttgatcaacc ccgggaataa cactcacatc
     4861 acgccatata aaggccgtac agcctgtctt tttgtttcct caaattacaa atgaaatatt
     4921 tttccggata atcaccacaa acacttgcgc gtttattaca aacgtgatat tatatagcat
     4981 ataaactgct gatgaagcgc gcaatcagca aaatcattgc atgacaattt gcggaattaa
     5041 ttataactgg gaggttatac gatgtttgaa ttcttgatga cgcctgaaca gttgaagctg
     5101 cgggatgaag ccagagatct ggccaaatca gttacacgcg attatatcct ggacatggac
     5161 gcagacaaaa tcaaatatcc caaggaatat ctgcgggaat gcggcagacg aggactgatt
     5221 ggtctcagag ttcccaagga atacggcgga cgtggactga aatgggaaga tgatatcatt
     5281 gctttggaag aaatctgtgt aaatggttat atgttcggtt gtagctatgg ggtcaccggt
     5341 gacgttgtac cagaggccat tgtaaacttc ggaacggaag aacagaagaa aaagttttta
     5401 ccctctattt gcaaaggaga aaaatttgct gctgaatgtt tgacagaacc acgaggtgga
     5461 tctgactttt ttggctcaac aacctatgcc agaagagacg gtgacgattt cattcttgat
     5521 gggcagaaac gctttatcgt cgggggggaa ggggcggatt atttcctggt ttacgccaag
     5581 actgatccgg aagccaaagc caacaaatcc atgacctgtt tcatggtgga acgcgaaatg
     5641 ggagtcaaaa cagagtatct gtacggtctg atgggatgcc ggggcggcgg aactgccaga
     5701 gtggtttttg acaatgtacg ggtacccaag gaaaacatta tcggcgaagt caacggtgcc
     5761 tatcccattt tctatcagat gatgattccg gaaagatttg gcacggcagc tatgacgatt
     5821 ggtgcggtac gtccggcttt ggaaatagcg acagattata ctttgaaacg caaggctttt
     5881 ggtcagacga ttaacaattt ccagggagta agttttaaaa ttgctgattc cgctgccaaa
     5941 ttggacagtt gccgcagctt tatgtatacc acagcccggg cggttgattc cggggcagat
     6001 cccaatctct gccggcgtct cgtctctgaa ggcaagaaat ttgttacgga acaatgctgg
     6061 gatgtagcca atctttgcat gcaggccatg ggcggcatcg gctatacaga tgtctatccg
     6121 ttggaaaaaa tcctgcggga tatgcgttta tcgatgattt gggtaggcag caatgaaatt
     6181 atgaatgtaa ttgttcaaca cgaatggtat acggaaagaa agaaagaact aaagaacaat
     6241 accaccaaac gggccagcga atttgatgct gccagcgctt ttgatgagga tgagaaaatc
     6301 tacgagtaaa cataaaaaac aggggatgca gcacatgcta taagaaacgg tgaaagtatc
     6361 agattttgaa atgcaaggag atgaaataat gtacgaaaaa tggcagcaga tgtataaaga
     6421 aaaattaaag actcccgaag aagcagcgca gatgattatc aatggagata aaatatccct
     6481 gccgactgcc aacggtacgc cccgcgtatt atggcaggcc attgccaaac gcattgccaa
     6541 tggtgaactg gttgacagcg aattaacggt gggcttaaac cttaatgcgc catatttatg
     6601 tacaccggag atagccagtc aaattcatta tcgggcgggt tatatcagtc cctttgagcg
     6661 tcccttggcc caaaaaggag tgatcgatcc ctggccggta aggttttttg atatccctcg
     6721 ttccatgatg gacagtgatt ataatgcggt taccatgact gccagtccca tggacgaaaa
     6781 tggctggttc agctgtgcga tgaactgcac ccacagttat tccctgacca ggcgggcgat
     6841 aaagctcggg aaaccaatga aagtattcct ggaagtcaat aaaaacgctc cccgtgtgta
     6901 tggacataat catttccata tttcagaggt taccgccgtt aacgaggcag actgggatct
     6961 gatttcattg cccagagcag aaattaccca ggaagatgtg gcaattgcca gttatatcgc
     7021 agagtacatt cccgacgggg cttgtgttca gcttggaatc ggaggtctgc ccgacgcagt
     7081 cggcaagcaa ttgaccagca aaaaagatct tggttgccac acggaaatga ttgttgatgc
     7141 ctatttggat ttatataaat ctggagcgct gaccaaccgt aaaaaaactt ttatggaaga
     7201 taaaatggtg ggcagcatca taattgggca accggaattg tataaatttg tggatcaaaa
     7261 cccaggcatt gaagtgcatg gtattgattt ttgtgctaat cctgccaata ttgccaaaca
     7321 cgataatttc atggccatca atgccattac cgaatgcgat ctgggtggtc agtgcatatc
     7381 ggaatccatg ggaatcgtac ctttttccgg gatgggcgga caggctgatt ttgtccaggg
     7441 agcctggcaa tccaaaggag gcaaagcatt cctggcgcta tactcaactt atactgacaa
     7501 gaagggtgaa ctccattcca aaatatcacc ggtggtaaac ggttgggtcg ggattactcg
     7561 ctgggatgta cagtatttgg caacagaata tggttgtgtc tatcttaaag gtaccagtat
     7621 gcgggaaaga gtacagcgga tcgtcagcat agctcatccg gatttccgtg attggctggt
     7681 tcacgaagcc aaacggctaa acctgattga ttctgcttct gatgtaaata ccaagcagat
     7741 gcgcaaagca atttaaaacc tcaaaccatc gaggaaaatg agtgggggca agcgtattgc
     7801 ccccactcat tttccgtatt tttaacaagt ccttgatttt tatatctgga tggtgacttg
     7861 tcataacgag cacttatctg caaaacccag ggctatcata acccgatttt gcattgcttt
     7921 actgcttgct cgaagtacca ttaatccatt ataattagca cataagtcga taccaactga
     7981 ggtggcaatc atgataacca aagaattggt ggaacgtatc aatgaactgg ccagtaaaaa
     8041 aaagagccag gggcttacgt ttgccgaaca agctgagcag aaaagacttt ataaaatata
     8101 tctggcctcc attcgggaac aggtcaagac ccagcttgac accatagaag ttgttccgga
     8161 gggacatgtc tgcgatgata cctgtcatca tcaccatcat ggtccaggct gcagccatga
     8221 ccatcatcag catggctctg acggcaatta taaaaaacac tgattcgagt accaaggcag
     8281 ttgggcagag ggccccgcaa aatttcctga caggaatacg ggcaggaggt tgcaatgata
     8341 gctaaacgta cggagttgaa tcgtatcatc ttgtatcgaa gcctgctgga ggacgaactg
     8401 gttaatctgg tctgtcagat gttggactgt gaaggtgcgg ccaataaagt caaattcgaa
     8461 gaatacttct atatgataag ccaccggctg cttgcctaca gccgttatta tggcacccag
     8521 ggagatgtct ggcaaaccta ctttctcgat ttgttgctgc aggacgaaaa cccgtacagt
     8581 ttaggatgcg aatgtagtgt ggatgacatt gatccgcagc tgcacaaaat ggcccgacac
     8641 gatttggaaa acctcaggga gctttattgc cttaactgga tgaacgtatg caagtctgct
     8701 tttattcgcc tggactgcaa ttatccttct tttcccggat taattccccg tgacccggct
     8761 cgccgggaag ccagggacaa gcttttc
//
LOCUS       DAOTHZ010000076         8672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_9745_length_8672_cov_6.838227, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000076 DAOTHZ010000000
VERSION     DAOTHZ010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9745_length_8672_cov_6.838227"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="P5549_08500"
     CDS             complement(<1..1067)
                     /locus_tag="P5549_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HRW13150.1"
                     /translation="MAEKIVEVIGIREENCTNCHQCIAVCPVKICSDGSGDVVKFNNN
                     LCIGCGRCVEACVKSHGGNSEKSARYIIDDAQQFSKDIGKKDIYCLVAPSAQSNFDLK
                     KLIAGLRKLGAKGVYDVSLGAEISVAACHEAIQSGTAKLPMIANPCPAVVRYIELEHP
                     DLIEYLAPVGSPLHCLAVYVRSLHPEAELAFISPCLAKRREIKENHLVKYNVTFQSLN
                     EIFQKKKINLTALPDGQYDNPIQAGIATKFSSPGGLKDSYLFHYPETPASRITKIEGP
                     IVFEQYLPNLEKAIKKGKPHLPLLIDVLSCEKGCNMGAGCINQKNSIDEIEYAVANRS
                     EICTSSPEKNKELDIFLRDVI"
     gene            1394..2956
                     /locus_tag="P5549_08505"
     CDS             1394..2956
                     /locus_tag="P5549_08505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRW13151.1"
                     /translation="MSSFSELNISSPILRALQEMGFESPTAVQCEAIPHILNQEDLIV
                     ISKTGSGKTAVFGVSMLQLINPQEAGPQGLILTPTRELAVQVDSDLKHIGKHLPHKTT
                     AVYGQHNINTEIDSLKKGVAIVAGTPGRVYDHIQHGTLKTNHIRFLVLDEADRMLDMG
                     FIDQVRRIIRTLPRNRTTLLFSATIPPEVQKLCRENMKNPALIEIESDTMTVDTTEQF
                     YYRVNDNEKRTQLNRLLQHERPDSCIIFCNMRSTVDRVQSFLTQKGIASRGLHGDIPQ
                     IKRLKTLQQFKEGVFHILVATDVAARGIHIDELALVINYDIPLEKDGYVHRIGRTGRA
                     GKVGRAISLVTSDDIMSLYEIEEHIGTIIEELPLPTDAALNEQQTLIAEWKKANEHKL
                     QPVRATGSTDKSRPKTKEAQGTARDSKPRSPKPTGNQPNKTDAQRQPKAPDKDGKPGR
                     RTASSGRQDLQKSSVPPASIVEQPKPQPSAPISHDQRPAPAPVHVETVAEKPAESKKS
                     WLERIKERFKRS"
     gene            complement(3118..3336)
                     /locus_tag="P5549_08510"
     CDS             complement(3118..3336)
                     /locus_tag="P5549_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13152.1"
                     /translation="MNRATKIAKRIAKKSPLALREAKQCINNAVNMDLRSGLKSEQIA
                     WAILFSSSDPQIGINAFLEGKEAIFKGK"
     gene            complement(3511..4602)
                     /locus_tag="P5549_08515"
     CDS             complement(3511..4602)
                     /locus_tag="P5549_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13153.1"
                     /translation="MLTKQRFPELSKKLGDCGVSFITDIRAEGRGAAIANTYFLDMGD
                     QYYMIDAACGKKRLRRIRDHLQGRNCDLLATHSHLDHSANSGAAVNQRSRIIFHPLVE
                     HKIDNLQRNYTEITPAMINAFGVEGFFGRTGMLRPSMRKKLLFIQKHFPSFFSQLMKL
                     VSLALLRINIGHIHSPHANVQYLSYADQRDLIFDGIHFSGWDLGNGIFALETPGHQND
                     HLAFYIPGRRIMFAGDLINFLNPNDILDGSIRDTHDGLNKMLQLAEAGAIDILALSHA
                     LPIVGSDQAVSYIRTIIAQQDEIFATVAEIVDSCADKNDFAEIIAKIYRHESELMKRV
                     LKINYPRSVSFIDVYVYLYLQEFLHPDID"
     gene            complement(4696..5058)
                     /locus_tag="P5549_08520"
     CDS             complement(4696..5058)
                     /locus_tag="P5549_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRW13154.1"
                     /translation="MNFIGNVIWLLLGGIIVAILWFLAGVILCITILGIPFGIQCFKI
                     AGFVLWPFGKEIELGHFGAGGLIMNIIWLIVFGWEFALTHLVIGLLFCVTIIGIPFGL
                     QHFKFAQLGLIPFGAVIK"
     gene            complement(5093..6496)
                     /locus_tag="P5549_08525"
     CDS             complement(5093..6496)
                     /locus_tag="P5549_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRW13155.1"
                     /translation="MTNTDSSQEKTALLTVMTTSFMTTFMGSSINLALPAMGNDLGSN
                     AVVTSWIITAYILASAIFLLPFGRLADIKGRRRVYLIGAIFYSLFTLLVPLANSVQMV
                     IALRALQGLSAAMIFGTGMAILSAVYPPQKRGKALGYSVSVTYLGLSAGPVVGGMMNQ
                     HLGWESIFYVSGLMGLAAVYGAYARLKGEWIGAPGERFDIPGALLYIIGLLALLYGLS
                     SVESSSWAIYLAAAGLILLAVFVKWELTSKEPILKIQLFRNNVTFTMSNLAALINYSA
                     TFAVTFLLSLYLQVVQGYSSQTAGLILLAQPLVMALLSPLAGRLSDRIEPRKVASVGM
                     ACSALSLFLFSFLRLDTSPVLLIGVLILAGFGFALFSSPNSNAVMSSVNIKLYGIASS
                     ILGTMRLVGQAASMSIVTLLVAVYIGRVALTDAPPADLLIMLRVAFGIFTVLCLFGVA
                     ASLARGKVHESNEKQAL"
     gene            complement(6520..6873)
                     /locus_tag="P5549_08530"
     CDS             complement(6520..6873)
                     /locus_tag="P5549_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRW13156.1"
                     /translation="MHELPVIDSILNIVLRHAANHQVQRVVSITLQVGAMSDLEDEWM
                     QRYFDQLSQGTVAEAAVLKINRIPVVFACPACGHEFEIFPHVNQAILCPQCGRDKGFT
                     LLSGREYHIKEMEVI"
     gene            complement(6899..7948)
                     /locus_tag="P5549_08535"
     CDS             complement(6899..7948)
                     /locus_tag="P5549_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW13157.1"
                     /translation="MSAMTMADEFKAYAKKLGFPESETMGRILDILFPDEISRKIAQA
                     LHKPVRIGELAEITAIPLDVLQETAEQLFCRGAIARYLDPPDQYRLYPAMIELRDATV
                     IHPDCPQALFELWEQLITKELADLTPYWKEKNVPEMLRVVPIEETVASQNLILDVDSA
                     RQIFREAEVIAAMPCPCRLQARRVGKEVDCPAPDDIDLCMQTNNFARAMLARGVGERL
                     SNEEALRRIELAEDAGLVHQVRNNIKEDMMMCNCCSCCCSGLYMIHHLDYTPYAKSRF
                     RVAFDASLCNGCGLCVKRCHFFAIDIDKNLKPKERVAQVDYDKCYGCGLCTLKCPTQA
                     LTMMEVRPREAVRTT"
     gene            8086..>8672
                     /locus_tag="P5549_08540"
     CDS             8086..>8672
                     /locus_tag="P5549_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRW13158.1"
                     /translation="MQRFSKMIAIMLLLAGVLLVAGCDLLRSDKDNGKTPTPATEEIN
                     VVVYYPKDTSYNSYLVRETHLLKKTDDMPKAAVEELIKVTPTTQGAYKVLPANTKVLG
                     VKVNDQGLATVDFSPEVLQANVGASGEALGIASIVNTLTEFPNIIKVSFTVNGKAENA
                     MDWWGHVGLYEQPFSRQMSLVQEPLIWVTEPIAGQA"
BASE COUNT         2394 a         2212 c         1990 g         2076 t
ORIGIN      
        1 ctgataacat cgcgcagaaa aatatccagt tctttattct tttcggggct gctggtacag
       61 atttcagagc gattggccac agcgtattca atctcatcaa tggagttttt ttgattgatg
      121 catcccgccc ccatgttgca gcctttttca cagctcagca catcaattag caatggcaaa
      181 tgtggcttcc ctttcttaat cgctttctcc agattgggta aatattgttc aaagacaatg
      241 ggcccttcta ttttggtaat cctgctggca ggcgtttccg gataatggaa aagatacgag
      301 tctttcagcc cgccaggaga ggaaaatttg gtcgcaatcc ccgcctgaat gggattgtca
      361 tattggccat ccggcaatgc cgtaagattg attttcttct tttgaaaaat ttcgtttagg
      421 gattgaaacg tcacattgta tttcaccaga tggttttctt tgatttctct gcgtttggcc
      481 agacatggcg atataaaagc cagttctgcc tcggggtgca gtgacctgac atataccgcc
      541 agacaatgca gagggcttcc aaccggcgcc agatattcaa tcaaatcggg atgttcgagt
      601 tcgatatacc tgactacggc cggacaggga ttggcaatca ttggcagttt ggctgtgccg
      661 gactgaatcg cctcatgaca tgcagcaacc gagatttctg cgcccagaga aacgtcataa
      721 acccctttgg ccccaagttt tctcagcccg gcaataagtt tctttaaatc aaagttactc
      781 tgtgcagatg gtgcaaccag gcaataaatg tcttttttac cgatgtcttt gctgaactgt
      841 tgcgcgtcat caataatata gcgggcgctt ttctcactgt tacctccatg gcttttaacg
      901 caggcctcta cgcagcggcc acagccaatg caaagattat tattaaattt aaccacatca
      961 ccactgccat cactgcagat cttcaccgga caaacagcga tgcattgatg gcaattggta
     1021 cagttttctt ccctgatacc gattacttca acgattttct ctgccattag catattccac
     1081 ctttattata agtttcgaaa cttctgctgt gatcggttgt gtatcgttac atttatggat
     1141 attattaata caaatcagta tgataacatg aagccttaaa atgctttaaa cttcaaattc
     1201 cattaacagt attgatctta ttttaaattc gacataattc cttatgactg taacaaaagt
     1261 gtaccatcta tttagctttt tctcaaccaa gatacacaaa aaatacaatc ctccaatcca
     1321 acagcagaga tgcttgaaaa gtgatataat attcaaggat tacctatcaa gaaaagcatg
     1381 aaagaagaca gacatgagca gttttagtga gctaaatatt tcatcgccca tattacgggc
     1441 tctccaggaa atggggtttg agtctccgac ggctgtccaa tgtgaggcaa ttcctcatat
     1501 attaaatcag gaagatttaa tcgtcatctc caagaccgga agcggcaaaa ccgctgtttt
     1561 tggtgtttcg atgctgcagt tgatcaaccc gcaggaagcc ggtccccagg gattaattct
     1621 cacgcccacc cgggaactgg ccgtacaggt ggacagtgat ctcaagcata tcggcaagca
     1681 tctgccccat aaaaccacgg cggtatatgg acaacataac atcaatacgg agattgacag
     1741 tttgaaaaaa ggcgtggcca tcgttgccgg aacgccaggc cgggtgtatg accatatcca
     1801 acacggcacc ttaaagacca atcatatccg ctttctcgtt cttgacgaag cggaccgaat
     1861 gctggatatg ggatttattg atcaggtcag gcgcatcatc cgtaccctgc cccgcaatcg
     1921 gacgaccctt cttttctccg ccaccatccc gccggaagtg cagaagctgt gtcgggagaa
     1981 tatgaaaaac ccggcgctga ttgaaatcga gtcggatact atgaccgtcg ataccaccga
     2041 gcagttttat tatcgtgtca atgacaatga aaaacgcacc cagctgaatc gtttgctgca
     2101 acatgaacgg cccgacagct gtatcatttt ctgtaatatg cgcagtaccg tcgaccgggt
     2161 gcagagcttt ctgacccaaa aagggatcgc ctcccgcgga ttacatggag atatcccgca
     2221 aatcaagcga ttaaagacct tgcagcaatt taaagaaggc gtattccata tactggtggc
     2281 tacggatgtg gcggcccggg gcattcatat cgatgagctg gccctggtga taaattatga
     2341 tattcccctc gaaaaagatg gctatgtcca tcggatcggt cgtaccggca gagcgggcaa
     2401 ggtcggtcgc gccatctccc tggttaccag cgatgacatc atgtctcttt acgaaattga
     2461 ggaacatatc ggcacgataa ttgaagaact gcccttgccg acagatgctg cgctcaatga
     2521 gcagcaaaca ctgattgccg aatggaaaaa ggccaatgaa cataagctcc aacctgtgcg
     2581 ggcaactggc agcactgaca agagcagacc taaaaccaag gaagcccagg gtactgccag
     2641 agacagcaaa cccagatccc ccaaaccaac cgggaatcag cctaataaga cggatgcaca
     2701 acgacagccc aaggcacctg ataaagatgg taaaccagga cggcggacag cttcatccgg
     2761 tcgacaggac ctacagaagt cttctgtccc accagcgagc atagtggagc aacccaagcc
     2821 gcagccatct gcgccaatct cccatgacca gcgtcctgcc cccgcaccgg tccatgtgga
     2881 aactgtggcg gaaaaaccag ctgaaagcaa gaaatcttgg ctggagcgga taaaagaacg
     2941 ctttaaacga agttaaaaac gaaaaagata attagaaaga tataaaaaac caagcataag
     3001 tagttaagcg caatgcaagc aggagaccgg tgattggagt gtacaggagg tacgccgctc
     3061 aaccatgctt cccttattga cgcaacgatc atccaatatt accagtctga gcggtattca
     3121 ttttcctttg aatatcgctt ctttgccttc cagaaaagca ttgattccga tttggggatc
     3181 tgacgaagag aacaggattg cccaggcaat ttgttcactt ttcaaaccgg aacgcagatc
     3241 catattgacg gcattattga tgcattgctt ggcttcgcgc aatgccaaag gcgatttttt
     3301 agcgattcgt ttggcaatct tggttgcccg gttcatcacg tcattgtcat ccaagcgcgg
     3361 atgatgttaa ccaggcatgg ggttctgatc tgcagcaagg gaggaagtca ctgaagttat
     3421 tgctcgatct ggaagcagat gtattatgcg agggacattg ggggatctat tccccggcct
     3481 cggaagtgcg ccgctatatc cagtcttatc ttaatcaata tcaggatgca agaattcctg
     3541 taaatacaaa taaacataaa cgtcaataaa tgaaacggaa cggggataat taattttcag
     3601 cactcttttc atcagttccg attcatgtcg atagattttg gcgatgatct ctgcaaaatc
     3661 atttttatct gcacaagaat ctacgatttc ggcaacagtt gcaaatattt catcctgttg
     3721 ggctataatc gtgcggatat aagagactgc ctgatcgctg ccgacaatcg gcagcgcatg
     3781 actgagcgcc agaatatcga ttgctcctgc ttcggccagc tgcagcattt tgttcaaccc
     3841 gtcatgggta tcccttatgc ttccgtccag gatgtcattg ggatttaaaa aattgatcaa
     3901 atccccggca aacatgattc ttcgcccggg aatataaaaa gcaagatggt cattctgatg
     3961 accgggcgtc tccagagcaa agatcccgtt gcccaaatcc catcccgaaa agtggattcc
     4021 atcaaatatt aaatcgcgtt gatctgcata ggaaagatac tgaacattgg catggggtga
     4081 atggatatgg ccgatgttga tccgcagcaa agcgagtgaa acaagcttca taagttggga
     4141 aaagaatgac gggaaatgct tttggataaa caacaatttt tttctcatcg aaggacgcag
     4201 cataccggta cggccaaaga acccctcaac tccgaaagcg ttgatcatcg ccggtgtaat
     4261 ttccgtgtaa ttgcgctgca ggttatcaat cttgtgctcc accagcggat gaaagatgat
     4321 ccgtgatctt tggttcacag ccgctccgct gttggcgctg tgatcaagat gggaatgggt
     4381 tgccagcaaa tcgcaattgc gaccttggag atgatccctg attcgcctga gacgcttctt
     4441 gccgcaggct gcatcgatca tataatactg atcgcccatg tccagaaaat aagtattggc
     4501 aatcgccgct cccctgccct cggcacgaat atcagtgata aacgaaaccc cacagtcacc
     4561 cagttttttt gatagctccg ggaatctttg ttttgtcaac atatgattat caatccccta
     4621 aatccgccag actgttgttt tcacgatccc ggcctgagtg ttattcccca tgaccatcaa
     4681 tctatccgtc cagccttatt taatcaccgc accaaaagga ataagtccca actgggcgaa
     4741 tttaaaatgc tgcagaccga agggaattcc tataatcgtc acacaaaaga gcagtccaat
     4801 gaccagatgg gtcaaagcaa attcccagcc aaatacgatc agccagatga tgttcataat
     4861 caatcctccg gcgccaaagt gacccagttc gatctctttg ccgaacggcc acaagacaaa
     4921 gccggcgatt ttaaaacatt ggattccaaa gggtatgccg aggatggtaa tacataaaat
     4981 gacacccgcc agaaaccata ggatggccac gatgattcct cccagtaaga gccagatgac
     5041 attgccgata aaattcatgc ttcttccccc attcccctta ttacattaat acttataatg
     5101 cctgtttttc gttggactcg tgaaccttgc cgcgcgccag ggaggcagcg acaccgaaca
     5161 aacacaaaac cgtaaaaatg ccaaaggcaa cgcgcagcat gatcaggaga tctgccggcg
     5221 gtgcgtcagt tagcgcgacc ctgccaatat agacggctac cagcaaggtt acaatggaca
     5281 tgctggcggc ttgcccgacc agacgcatgg tccccaggat ggatgatgca atcccgtaaa
     5341 gctttatatt cactgaactc atgaccgcat tgctgttggg agaagaaaac agggcaaagc
     5401 caaaaccggc caggattaaa acaccgatta acaggaccgg agaagtatcg agccggagaa
     5461 aagaaaagag aaacaaactt agcgccgagc aggccatgcc cacagaagcg actttgcgtg
     5521 gttcaatccg atccgacagc cggcctgcca gaggcgagag cagcgccatg accaacggtt
     5581 gggccagcag gattaaaccc gctgtttgag acgagtaacc ctgcaccacc tgcagataaa
     5641 gcgacagcag aaaggtcacc gcaaaggtag cgctgtaatt gattaaagcc gccagattgg
     5701 acatggtaaa ggttacattg tttcgaaaca gctggatttt cagaatcggt tccttgcttg
     5761 tcaattccca tttcacaaat accgccagca gaatcagtcc cgctgccgcc agataaatag
     5821 cccaagacga gctttccacc gatgacaggc catacaacaa ggccagcagt ccaataatat
     5881 agagcaatgc tccgggtata tcaaaacgct ctcccggcgc ccctatccat tcccctttca
     5941 gtctggcata agcgccatac acggccgcca atcccatcag cccggataca taaaatatgc
     6001 tttcccagcc cagatgctgg ttcatcatgc cgcccaccac cgggcctgcg gataatccca
     6061 gataggtcac ggatacactg tagcccaaag ccttgcctct cttttgggga gggtaaacag
     6121 cgcttaaaat ggccataccg gtaccaaaaa tcatagccgc gctgaggccc tgcaaggcgc
     6181 gcaacgctat gaccatctgt acggagttgg ccagcggcac cagcaaggta aacaaggaat
     6241 aaaaaatcgc cccgatcaga taaactctgc gccgtccttt aatatcggcc aggcggccaa
     6301 aaggcaggag aaaaatagcg gaagccagga tataggccgt gataatccag ctggtgacga
     6361 ccgcgttgct gcccaaatca ttgcccatgg caggcagggc cagattgatc gaactgccca
     6421 taaaggtggt cataaacgaa gtcgtcatta ctgtcaataa agctgttttt tcttgcgagc
     6481 tgtctgtatt cgtcaaattc gccccatttc ctgataaaac taaattactt ccatttcctt
     6541 gatatggtat tcccgcccgg aaagcaaggt aaagcctttg tcccgcccgc actgcggaca
     6601 gaggatggcc tggttaacat gcggaaagat ctcaaattca tggccacagg ccggacaagc
     6661 aaacactacc ggaatgcggt tgatcttcaa caccgcagct tctgccaccg ttccctgact
     6721 gagctggtca aaataacgct gcatccattc atcttccaaa tcactcatcg caccaacctg
     6781 cagggtaata gaaaccaccc gctgcacttg atgattggcc gcatgccgaa ggacgatatt
     6841 tagtatactg tcaatgaccg gcagttcgtg catggccttg ccccccttgg cagaactttt
     6901 atgttgtgcg caccgcttca cggggacgca cttccatcat ggtcagcgcc tgggtgggac
     6961 atttcaaagt gcacaagccg cagccataac acttgtcata gtcgacctgg gctacccgtt
     7021 ctttgggttt cagatttttg tcaatatcaa tggcaaagaa gtggcagcgt ttcacgcaca
     7081 gaccgcagcc gttgcagagg gatgcatcaa atgcaacccg gaaacgcgac ttggcataag
     7141 gcgtgtaatc caaatgatgg atcatatata atccactgca gcaacaggaa cagcaattgc
     7201 acatcatcat gtcttcttta atattattgc gcacctgatg aaccagtccg gcatcctcgg
     7261 ccagttctat ccgccgcagc gcttcctcat tgcttaaccg ctccccgact ccgcgcgcca
     7321 gcatggcccg ggcaaagttg ttggtttgca tgcagagatc gatatcatcc ggcgccggac
     7381 aatcgacttc ttttcctacc cggcgggcct gcagtcgaca aggacaaggc atggcggcaa
     7441 tgacttccgc ctcccgaaag atttgccggg ccgaatccac atccagaatc agattctggg
     7501 atgcaaccgt ttcttcaatc ggcaccaccc gcagcatttc cggcacgttc ttttccttcc
     7561 agtatggcgt gaggtctgcc agttctttgg tgatcaactg ctcccatagt tcaaacaacg
     7621 cctgtggaca atcaggatga atgacggtgg catcccgcag ttcgatcatc gccggataca
     7681 accggtactg atcaggcgga tccagatatc tggcaatggc tccccggcaa aacaactgct
     7741 cggccgtctc ctgcaagaca tccagcggta tcgcggtgat ttctgccagc tccccaattc
     7801 tgacaggttt atgtagagct tgggcgattt ttcggctgat ctcatccggg aacaatatat
     7861 ccaaaatccg gcccatggtt tcagattccg gaaaaccaag ttttttcgca tacgctttaa
     7921 attcatcggc cattgtcata gcgctcatcc tctcctgctt ccaatccgta caaaattcac
     7981 actaatgaaa ttgtataagt tagctatact aattacaagc aggatgaaag gttttgcccg
     8041 aatcttctct attatgacta taagaattac atggagggat ttaatatgca gcgcttttct
     8101 aagatgatag ccatcatgtt gctcctggca ggggttttgc tggtggcggg ctgtgatttg
     8161 ctcaggagcg acaaagataa tggcaaaact ccaacacctg ccactgagga aatcaatgtg
     8221 gttgtctatt atcccaaaga tacatcgtac aacagttatc tggtgagaga aacccatttg
     8281 ctcaaaaaga cggatgatat gcccaaagca gccgtggaag agctgattaa ggtaacgccg
     8341 acaacgcagg gcgcatacaa ggttttgccg gccaacacca aggtcctggg agtgaaagtc
     8401 aatgaccagg ggctggccac cgtcgatttt tcgcccgaag tattgcaggc caacgtggga
     8461 gccagcggtg aagcattagg catcgccagc atcgtcaaca ccctgaccga gtttcccaac
     8521 atcattaaag tatcctttac ggtcaatggc aaagcggaaa acgccatgga ttggtggggg
     8581 catgtgggtt tgtatgagca gcctttcagc cgccagatgt cattggtcca ggaacctttg
     8641 atctgggtca ccgagccgat tgccggccag gc
//
LOCUS       DAOTHZ010000077         8597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_9854_length_8597_cov_7.982557, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000077 DAOTHZ010000000
VERSION     DAOTHZ010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9854_length_8597_cov_7.982557"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="P5549_08545"
     CDS             complement(<1..1370)
                     /locus_tag="P5549_08545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRW13159.1"
                     /translation="MLKFQELKIDPTIISALSNMGFEEPTPIQEMTIPLAMMGRDLIG
                     LAQTGTGKTAAYGIPLVDKCSAETEQVQGLVIVPTRELAMQVAEELNKIGSQKKIRSL
                     PIYGGQEMERQIRALKNRPQIIVGTPGRLMDHLRRRTIRLDQISLLVLDEADEMLNMG
                     FKEDIEAILRHAPQERQNLLFSATMPLHTRNLSQEFMQNPEIIHINPRAVTVINTEQQ
                     YMEVPELKKLDVLCRLLDMQNPDMAIVFGRTKRRVDEIAEALSKRGYSADGIHGDLTQ
                     SQRDTVMRKFRTGTIDILVATDVAARGLDISGVTHIYNFDIPQDAEWYVHRIGRTGRA
                     GETGLAITFVTSREMGHLKYIEESIKHKISRQPMPTPNDALAGLQQAVVNNLLEVAGQ
                     VEMQAYRALAKGLLDEYDSETLLAAALKIMTREPEEKPLPVLTEAPPIHLKRVRTPKE
                     NNSGYRR"
     gene            complement(1490..1798)
                     /locus_tag="P5549_08550"
     CDS             complement(1490..1798)
                     /locus_tag="P5549_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HRW13160.1"
                     /translation="MYSDKTLTCRDCGNDFAFTASEQEFYAEKGFTNEPGRCPECRAA
                     RKAESRGGNRGGYQSDRQMYEATCATCGKIAMVPFKPSGDRPVYCKDCFTPQPRRNSW
                     "
     gene            complement(2152..2943)
                     /locus_tag="P5549_08555"
     CDS             complement(2152..2943)
                     /locus_tag="P5549_08555"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HRW13161.1"
                     /translation="MERVSRGDYDSRILLETDDEFSNIARHFNEMTERIKASMADQQA
                     KEEEIKTLYKNEASMNETLLQMMASNKQGYFETIKALVNAEDEKDAYTKGHCERVMNY
                     SLNIGKSINLTEKDMDILRFGAILHDLGKIGIPEQILNKGTALTNEELALIRQHPSIG
                     ARILNDLTFMQDSIRIVHEHHEWHDGHGYPRKLKGEEIDLLARIVCVADAFDAMTTQR
                     PYRKKAMTIVEAIQELRDKSGTQFDPGLVKVFVSLLEQDLIENLA"
     gene            complement(2948..3943)
                     /locus_tag="P5549_08560"
     CDS             complement(2948..3943)
                     /locus_tag="P5549_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13162.1"
                     /translation="MKSLQSLKSKLIILLIGVVLLTTILLGAFQYFFVMKNYETLDLQ
                     RRALLEDNILKLMKATDSVYQMVEAPIEKESQVILARMVADYNQKGLSGIDLHDYYDE
                     ESGLELYIIDQSDTVIAATYKPDIGLNFSKFAGVDEFLDGIRDEGKFFSDRLSLSSLE
                     NIVMKYSYLPTPDGMYIFETGSPIDKDKAIPDVLSFGDFGDSISKNQGFVVSALLFSK
                     YGVTFNEDVSEVKQINPSFKPYYNKALDTLQPVTFEGTYDGQAVTIKYLPFSFAGAGD
                     LHEHTVIELIYSNADLQKSKNQVILLMLLTILLGSGLIAALAVRMSTGFSPGHPA"
     gene            complement(4357..5469)
                     /locus_tag="P5549_08565"
     CDS             complement(4357..5469)
                     /locus_tag="P5549_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW13163.1"
                     /translation="MKRKAITFIITAVIMWAMLAILPAFAADLPTGSNFDKAPQNLTV
                     EVKQWADGKPYFALQWTNPESIWELQQYGVEHGEWGAEYQIDMKIGDGQWLHEAGESL
                     FGNGINFDEELDEPGNYVTDDVTCDPVDMGVNETIDIKTNVYYLRVRYAYSANQGSDD
                     EHYIYSPFSNVASIGMEAFYKEASEWAKSELQGANDLGLIPAILKGADMTKPITREEF
                     CELAVLLYEKVTGQKSEPVASNPFTDTSNLQILKAYNLGITAGTSPTTFSPNVLINRE
                     QCAAMLFRAIKAIHPQGDYNVTGTPDFPDQKYISDWAVEATKYMNKIGIIAGDAQGNF
                     MPKATTPAQEAAGYGMATREQAIALSVRTYEKAPTI"
     gene            complement(6058..8046)
                     /locus_tag="P5549_08570"
     CDS             complement(6058..8046)
                     /locus_tag="P5549_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HRW13164.1"
                     /translation="MEPAELGSFRKYTGKAGNILPASGKKRWFLSIITIAIVLLLSII
                     NGPAMVFAESSSLYWTDYASQPTLSGTTYTIASAAQLAWVAKQVNAGNDFAGYTIEIS
                     DGLSSIDLSAHDWEPIGYTTNGANVDRSFSGIFDGNKIPISNLKIGTFESPETKLYYV
                     GLFGYAKKAEIKNTCITNAAVYYSQDASLSSAGSLIGYGYDSIITECSSTGTVTCGNA
                     AAAGGLAGRLDSCVAEDSWSLCAVTVETSADPAYVTQYAGGFAGAVNNSTISGCFHTT
                     GNVQSEKGPAHVGGLIGNAESVVGSYGCFYSGTVFIKNCYATGEVTGGAASSVGGLAG
                     SLTNGFVTGSHASVRVTGGDTNTEGTDDTSFVGGLVGYACTLDSEGNPDLVATDCYAT
                     GEVSGTKNCFVGGLAGFSSDLTDCHAAGSVTGGNGSNVGGLAGCACDVTDCYAIGNVV
                     STSTGAFVDLGGLAGYVNNVTNCYATGNVKGAADCRIGGLAGQACGDIKNSYATGSVI
                     TEGEAIDAGGLAGMVDGAGNDLVNSCATGNVSIERGNAGGLASEIVGDIINCYAAGAA
                     SSAEPDRQLGGLLWAGMYGTSVSPAYWNSDANSIGVEFCQDDYDYNTGTEIKFADTST
                     GVPSAYMQSSAFVDELNAFAGSHAGLKVYRHGGLWLKV"
     gene            complement(8349..>8597)
                     /locus_tag="P5549_08575"
     CDS             complement(8349..>8597)
                     /locus_tag="P5549_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRW13165.1"
                     /translation="QVEDEHFRKARISCQTHRGKLLIFIENSFRGEVDMLNGLPQSGR
                     EGHGFGVKSMAMIAEKYNGYCSFEAKGEIFTLRIVLPL"
BASE COUNT         2114 a         2252 c         1857 g         2374 t
ORIGIN      
        1 cggcgatagc cgctattgtt ctctttcgga gtgcggacac gcttgagatg aatcggcggc
       61 gcctccgtca aaacgggcaa gggtttctct tccggttcac gggtcattat tttcagagca
      121 gcggccagca aggtttccga atcatattca tccagcaagc ccttggccaa agcccgatag
      181 gcttgcatct ctacttgacc ggcgacttcc agcaaattgt tcaccaccgc ctgctgcaga
      241 cccgccagcg catcattggg ggtaggcatg ggctgacggc tgattttgtg tttgatggat
      301 tcttcaatgt atttcaagtg acccatttcc cgggacgtca caaaggtaat ggccaagccg
      361 gtttcgcccg cccgaccggt cctgccgatg cggtgaacat accactctgc atcttgcggt
      421 atatcaaagt tatagatatg cgtgaccccg ctgatatcga ggccccgggc ggctacatca
      481 gtggccacca gaatatcaat cgttccggtt ctgaatttcc gcatcaccgt atcccgctga
      541 ctctgggtca aatcgccatg aatcccgtcg gctgaatatc ctcttttgct taaggcttca
      601 gcgatttcat caacccgacg cttggtacgg ccaaagacaa ttgccatgtc gggattctgc
      661 atatccagca ggcggcacag gacatccagt ttcttgagct ccggcacttc catatactgc
      721 tgctcggtat taatgacggt cactgcccgg ggattgatat ggattatttc cggattttgc
      781 atgaactcct ggctgaggtt gcgcgtatgc aagggcatgg tggcggaaaa caacaggttc
      841 tgccgttcct gcggcgcatg ccggaggatc gcttcgatat cctctttgaa gcccatattg
      901 agcatttcgt ccgcttcatc caagaccagc aggctgatct gatccagtcg aatcgtgcgc
      961 cggcgcagat gatccatcaa tcggcccggc gttccgacga tgatctgggg acggtttttc
     1021 agcgcacgga tctggcgttc catctcctgt ccgccataaa taggcaggga acggattttt
     1081 ttctggctgc cgatcttgtt gagttcttct gccacctgca tcgccaactc gcgggtggga
     1141 acgatcacca acccctgcac ctgctctgtt tcagccgagc acttatccac cagaggtatt
     1201 ccataggcag cggtttttcc ggttccggtt tgggccagcc caatcaggtc gcgccccatc
     1261 atcgccaagg gaatggtcat ctcctgaatc ggcgtgggtt cttcaaaacc catgttactg
     1321 agtgcagaga taatggtcgg gtcgatttta agttcttgga attttaacat atgttaatga
     1381 ttctcctaaa atattttttg tttaatatga ataaaccttg ggcaaattaa tcggctcaca
     1441 aaaaatccct ggagccgcga acgattccag ggattccatc aatcaaattc taccagctat
     1501 ttcttcttgg ctgcggtgta aaacagtctt tgcagtatac tggtctgtca ccgctgggct
     1561 tgaagggtac catcgcgatt tttccgcaag tcgcacaagt cgcttcgtac atctgacggt
     1621 ctgactggta accgccgcga ttgcctcccc ttgattcagc cttgcgcgca gcgcggcatt
     1681 cggggcagcg acccggttca ttggtaaatc ccttttccgc atagaattcc tgctcggaag
     1741 cggtgaacgc aaaatcgttc ccacaatctc tacacgtaag tgttttgtct gaatacataa
     1801 aaaaacctcc taaattttct gctcatatca aaggtagtac ctgtcgcaac tccctttaat
     1861 gcagaaagta ttcagaaggt ctattcatcc ttaatgtatc gagcttggta gattgtaaca
     1921 gatacttgcg taaaaagcaa atatactttt tttgtgtgcg attggtgaaa acacgcgtgc
     1981 tacagcagag gcgatgtaaa tcgatagtta tcgcttacaa cagatatgtt atcaaggaat
     2041 aaactccaaa tcctgccgcc agcagtgcat acatcataat tcaaggcgga cactttttat
     2101 accagggaat ttttttctcc gtcatatggt atcatccttt cctgataata actaagccaa
     2161 attttcaatt aaatcctgct ccagcaggga aacgaatacc tttaccagac cgggatcgaa
     2221 ttgggtgccg cttttgtccc ttagttcctg gatcgcttcc acaatcgtca tcgctttctt
     2281 acggtaaggc ctttgggtgg tcatcgcgtc aaaggcatct gccacacaca cgatccttgc
     2341 gagaaggtct atttcttctc ccttgagctt ccttggataa ccgtgtccat catgccactc
     2401 atggtgttca tgaacgattc tgatgctatc ttgcataaag gtaagatcat tcagtatcct
     2461 tgcgcctatc gaagggtgct gtctgattaa ggcaagttcc tcgttggtca atgccgtccc
     2521 cttgttgagg atctgttccg gtattcctat cttgccaaga tcgtggagaa tagccccaaa
     2581 cctcagtata tccatatcct tttcagtcag gttgatcgac ttgccgatat taagcgagta
     2641 attcattacc ctttcacaat gccccttcgt ataagcatct ttttcatcct cagcattaac
     2701 gagggctttg atggtttcga aatagccctg cttgttcgac gccatcatct gcagcagcgt
     2761 ctcgttcatg gaggcttcat ttttatacag cgtctttatc tcctcttcct tggcctgctg
     2821 atccgccatg gaagctttga ttctttcggt catctcgtta aaatgtcggg cgatattgga
     2881 gaactcatcg tcggtttcca ataggatcct ggaatcataa tcgccccggg atactctctc
     2941 catgccgtca tgcaggatgt ccaggggaga aaccagtgga catcctcacg gcaagggcag
     3001 cgataaggcc agagcccagc aatatcgtca gcagcatcag caggatgact tgattctttg
     3061 atttttgcag atcagcattg ctgtagatta gctcaattac ggtatgctca tgaagatctc
     3121 ctgctcctgc aaagctaaag ggcaaatact tgatcgttac cgcttgtcca tcataagtac
     3181 cttcaaaggt gaccggctgc aaggtgtcaa gtgccttgtt gtaataaggt ttgaaggaag
     3241 gattgatttg ttttacttcc gacacatcct cattaaaggt gactccatac ttgctaaaca
     3301 gcagcgcact tactacaaag ccctgattct tggaaatgct atctccaaaa tctccaaagc
     3361 ttaatacatc cggtatagct ttatccttgt ctatcgggct gccggtttca aagatgtaca
     3421 tcccatccgg tgtaggcaga tatgaatact tcatgacgat gttctcgaga ctcgacaacg
     3481 agagcctgtc actgaaaaat ttgccttcat ctctgatacc atccaaaaac tcatcaacac
     3541 ctgcaaattt tgaaaagtta agccctatat ccggtttata ggtcgctgct atgactgtgt
     3601 cggattggtc gatgatgtag agttcgagac cgctttcctc gtcgtaatag tcatgtagat
     3661 caataccaga cagccctttt tgattataat ccgcaaccat cctcgccagg attacttggg
     3721 attccttctc gatgggggct tccaccattt gatagacgct gtcggtggcc ttcatcagct
     3781 tcaaaatatt atcttccagc agtgcacgtc tttgcagatc caacgtttca tagtttttca
     3841 tcacaaaaaa atactggaac gcgcccagta gtatcgtggt gagtaaaacc acaccgatca
     3901 acaggatgat cagcttgctt ttaagacttt gcagggattt cattgatcta agcatgttct
     3961 tcccctttat atatggattt tactcttttc atttgtccgc ccgggaaagt gcaaacatcc
     4021 aattgaatta ttttttctgt tggaaaggaa tcggcaatta cttcgtcacc tgacgcttgc
     4081 tgcgcctcgt acgtctccga ttcggtcccc gtttataaat aaattatatc acagcttaat
     4141 aattttgatc gggtatattt catgccaatg atatcgctct tccagaagca gctgccatgc
     4201 tgcaaaggtt tattgaaaac gtaagcaatc gataatgact gaaagagaca acagaatcta
     4261 ctgaaataaa attacgggga ggaagtcact cattctctga gtggcctcct ccccgcttct
     4321 tgtcaaacga tccgcaatcc caacacagac ccgtttttaa attgttggtg ctttctcata
     4381 ggttcttact gataaagcga ttgcctgttc tcttgttgcc atgccgtacc cggcagcctc
     4441 ctgcgccggc gtggtggcct tgggcataaa attaccctgc gcgtcgccag caataatgcc
     4501 gattttattc atatatttag tagcttcaac cgcccagtcc gaaatgtatt tttgatccgg
     4561 aaaatccggg gtgcccgtta cattataatc tccctgggga tgaatcgctt ttatagctct
     4621 aaacagcata gccgcgcatt gttcccggtt aatcaagaca ttcggcgaaa aagtggttgg
     4681 agaagttccc gcggttatgc cgaggttata tgctttcaat atttgcaggt ttgacgtatc
     4741 cgtaaaggga ttcgaagcaa ctggctcgct tttttgtcct gtcacctttt catacagcaa
     4801 aacggcaagc tcgcagaatt cttcccgcgt gatgggtttg gtcatgtcag cgccttttaa
     4861 tatggcaggg ataaggccaa gatcattggc cccctgaagc tcagacttgg cccattcgga
     4921 cgcttccttg tagaacgctt ccataccgat agaagcaaca ttggaaaaag gactgtatat
     4981 ataatgttca tcatcgctgc cttgattggc agaataggcg tatcgcaccc gcaaataata
     5041 gacatttgtc ttgatatcga tcgtctcatt tactcccatg tcaacagggt cgcaggtaac
     5101 gtcatctgtt acataatttc cgggttcatc taattcctca tcaaaattaa tcccattccc
     5161 gaaaaggctc tcacccgctt catggagcca ttgtccgtca ccaatcttca tatcgatctg
     5221 atactctgcc ccccactctc catgttcaac gccgtattgt tggagttccc atatgctttc
     5281 agggttcgtc cactgtaacg caaaatatgg tttcccatct gcccattgct tgacctccac
     5341 cgtcaaattt tgcggggcct tgtcaaagtt gctgcccgtc ggcagatcag ccgcgaatgc
     5401 cggcaaaatc gcaagcatgg cccacatgat caccgcggtg atgataaaag ttatagcttt
     5461 ccttttcatt gatcgtacct ccccatagtc attttttggt ttgtttttct cttggcggtc
     5521 aagaatccgt ttttccttgt ccggcccggt gtgttttgct catgacataa acaccataac
     5581 gttgcgggtg cattcagtat catcagccga aacaggaaaa atgacggttc tgttgaacca
     5641 gacaggttat ctatatttta ttggatggct ggccgtaagg ataggttgaa tatgcttctt
     5701 atatagtaaa aatagtgtta ttttgaggtt tattgaaaac gtgagcgatt gataattacc
     5761 aaattcccaa tataattccg atactaagct tgaacatatc ttctgcgcaa ccagtacaaa
     5821 caaattaaga atataatcaa cagcgctatt cccatcagta ttgggtgata tatcatatat
     5881 accatggcta tgaatggagc caacaatacc ctgaccaata ccttcagcgg ctggctgccg
     5941 gcgataagtg ccgctatggg gggtgaatat ttataataat aattcacaaa ggtcgtcccc
     6001 aaggaattgg tcaacaaata ctgatcacgg aaatgcctca gcagagccac cggccaatca
     6061 aactttgagc caaaggccgc cgtggcgata aacctttagc cctgcgtgag aacccgcaaa
     6121 tgcattcaac tcatcaacaa aggcgctgga ttgcatgtag gccgatggca caccagtgct
     6181 ggtatctgcg aatttgatct ccgtcccggt gttgtagtca tagtcgtcct ggcaaaactc
     6241 gaccccaata gagttggcgt cgctgttcca atatgcaggg ctgactgaag tcccatacat
     6301 tcccgcccac agcaagccgc caagctgcct gtccggttcg gctgaggacg ccgccccggc
     6361 agcgtagcag ttgataatat cgccgacaat ctcagatgcc aggcctcctg catttcctct
     6421 ttcaatgcta acattgccag tggcgcagct attgaccaag tcattccctg caccgtctac
     6481 catgccggcc aggcctcccg catctatcgc ctcgccttct gtaatcacac ttcctgtcgc
     6541 gtaggagttc tttatatcgc cacatgcctg ccctgccaat ccacctattc tgcaatctgc
     6601 cgccccttta acattgccgg ttgcataaca gttggttaca ttgttcacgt atcccgcgag
     6661 acctcccagg tcaacgaaag caccagtgct ggtactgacc acatttccga tcgcatagca
     6721 gtctgtaaca tcgcacgcgc atcccgcgag tcccccgaca ttcgaaccat taccacctgt
     6781 cacactaccg gcagcatggc agtctgttaa gtcgcttgaa aatcctgcca aaccgccaac
     6841 aaagcagttt ttcgtgcctg acacctcgcc ggtcgcatag cagtctgtcg ccaccaagtc
     6901 ggggttcccc tcgctgtcca aggtgcaagc gtaccccacc aggcccccca caaaggatgt
     6961 atcgtcagta ccctcggtgt ttgtgtcacc gcctgttacc ctgacactcg catgactgcc
     7021 agtcacaaag ccattggtca acgatcctgc cagaccacct actgaagaag ctgccccgcc
     7081 cgtcacttca cctgtagcat agcagttctt gatgaatacg gtgccggaat aaaagcagcc
     7141 ataggaacca accaccgatt cagcgtttcc tatcagcccg cctacgtgcg caggaccctt
     7201 ctcactctga acattgccgg tcgtatgaaa acagcccgag attgtactgt tattgacggc
     7261 accggcgaat ccacctgcgt actgggtcac ataggcggga tctgcactgg tctcaacggt
     7321 caccgcacac aacgaccagc tgtcttcagc cacacaagaa tccaaccgtc ccgccaaccc
     7381 gccagctgct gcagcattac cgcaagtaac tgtgcctgtg gaggaacatt cagttatgat
     7441 gctatcatag ccatatccga tcaggcttcc tgctgaagac aaagatgcgt cttgtgaata
     7501 gtagactgct gcattagtaa tacaagtatt cttgatctct gcctttttcg catacccaaa
     7561 cagcccgaca tagtaaagtt ttgtttccgg gctctcgaaa gttccgatct tcagattgga
     7621 tatcggtatt ttgtttccgt caaaaattcc tgaaaagcta cgatcaacgt tggctccgtt
     7681 tgtggtgtag ccaatcggtt cccagtcatg agctgacagg tcaatagacg acagtccgtc
     7741 tgaaatctca atcgtatagc cggcaaagtc attccctgca tttacctgct tcgcaaccca
     7801 cgcaagctgg gccgcgcttg caatggtata ggttgtacca gacagcgtcg gttgggaagc
     7861 atagtctgtc cagtaaaggc ttgatgattc ggcaaaaacc atagccgggc cgttgataat
     7921 cgataataat aaaactatcg caatcgtaat gatcgataaa aaccaacgtt tctttccgga
     7981 ggcaggaagg atattccctg cttttcccgt atatttcctg aagcttccaa gttcagcggg
     8041 ttccatggca acacctcttt cagtccttgt gtaatctgta aaagtatttt ttaaaataaa
     8101 atacaactat attttactac caaaattggt atttttgtgt ttttttcagg gggttgcccg
     8161 gaatattgaa aacgaaccgt ttcctcaaaa cgtcctgctt ctaatgttgg tttcgcataa
     8221 actctaaggc atttgtcgat gaggggggat gacttgcaga tgttaacaac ttaaaagaac
     8281 gtctaaacat acagtaaggc ctcttcacaa taaagaggcc cataaatatt ctcaatttaa
     8341 ccccatcctc acagcggcag cacaatccgc agggtaaata tttcaccctt ggcctcaaag
     8401 gagcagtagc cgttatattt ttcagcaatc atggccatgc ttttcacccc aaagccatgc
     8461 ccctcccggc cgctttgcgg caagccgttc agcatatcca cctcacccct aaagctgttc
     8521 tcgataaaaa tcaacaattt gcccctgtgg gtttggcagc tgattcttgc tttcctgaag
     8581 tgttcatcct caacctg
//
LOCUS       DAOTHZ010000078         8438 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_10113_length_8438_cov_8.388882, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000078 DAOTHZ010000000
VERSION     DAOTHZ010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10113_length_8438_cov_8.388882"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="tyrS"
                     /locus_tag="P5549_08580"
     CDS             <1..1041
                     /gene="tyrS"
                     /locus_tag="P5549_08580"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRW13166.1"
                     /translation="NKPVVLIGGGTAYIGDPSGRSDLRSMMTPEIIKHNSECFKQQMQ
                     KFIEFGDDKAIMVNNADWLLQLNYVELLREVGNCFSVNNMLRAECYKQRMEKGLSFLE
                     FNYMIMQSYDFYYLFQHYGCNMQFGGDDQWSNMLGGTELIRRKLGKDAYAMTITLLLT
                     SEGGKMGKTAKGAVWLDPAKTSPYEFYQYWRNIDDADVLKCLRMLTFLPLEQIDAMDQ
                     WEGSQLNVAKEILAYELTKLIHSQEEAEKAQSTSRALFAGGIDVNMPTTEIAVGQLEN
                     GVIGMVELMVMSKLAASKSEARRLIQQGGVFVDDVKVESADAMITAKQLKNGIKIRKG
                     KKTYHKIYLKQQ"
     gene            complement(1157..3748)
                     /gene="clpB"
                     /locus_tag="P5549_08585"
     CDS             complement(1157..3748)
                     /gene="clpB"
                     /locus_tag="P5549_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRW13167.1"
                     /translation="MNIERYTKKSQEALGTAQQLAASRHHQEVSGKHLLQALLQQDGG
                     LTPNLLEKAGVQLPGLSKQLQDLLDKLPSIHGYEGQVYLSGSLARVLSKAEQEAAALK
                     DEYISVEHLLLGLIQEGESDLRDLLARLGINRNSLLVALKNIRGSQRVSSDNPEDSYE
                     ALQRYGRDLNELARQGKLDPVIGRDEEIRRVMEILSRRTKNNPVLIGEPGVGKTAIVE
                     GLARRIVARDVPEGLKEKTVIALDMGSLVAGAKYRGEFEERLKAVLKEVEQSDGKIIL
                     FIDELHTVIGAGAAEGAMDASNLLKPMLARGELRAIGATTLDEYRKHIEKDAALERRF
                     QPVLVNPPSVEDTISILRGLKERYEVHHGVRIKDAALVAAAVLSDRYIADRFLPDKAI
                     DLMDEAAARLRTEMDSMPTELDEITRRIMQLEIEATALGKENDTASWERREHIVKEKQ
                     DLQTEADAMQAKWQSEKNAIARVSQLKRDIEDCRMEIEKAERDYDLDKAAELKYGRMN
                     ELERKLQAEEATLADKQGQQMLLQEEVGEEDIAKVVSRWTGINVSRLLEGERQKLMHL
                     DEILHQRVVGQDEAVQAVADAVLRSRSGLKDPNRPVGSFIFLGPTGVGKTELAKALAE
                     SLFDDERAMIRMDMSEYMEKHSVARLIGSPPGYVGYDEGGQLTEAVRRRPYSVVLFDE
                     IEKAHNDVFNILLQILDDGRLTDGQGRTVDFRNTIIIMTSNLGSQEILQFQEQGGRYD
                     LMKEAVISILKQYFRPEFLNRLDEIIVFQALSTEQIEKITDLLLQTLARRFEKNLGGQ
                     LQWDEKALQYLAEKGYHPAYGARPLKRLIQQEVETLLSRKIISGEIKENSPVQLSASP
                     AGITLKV"
     gene            complement(3754..4029)
                     /locus_tag="P5549_08590"
     CDS             complement(3754..4029)
                     /locus_tag="P5549_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HRW13168.1"
                     /translation="MTLIKIYYENSDELIPLSRLEYSPDFLRMLAEMGIITLKHDNIS
                     CSELKKVNRLLRLRNWLGVNVSGAAIILDLLDRIDALEAEIQSLKRR"
     gene            complement(4033..5022)
                     /locus_tag="P5549_08595"
     CDS             complement(4033..5022)
                     /locus_tag="P5549_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRW13169.1"
                     /translation="MAVSYQDYYEILGVPRDASAKDIKAAYRKLARQWHPDLHTGKKK
                     VEAEEKIKRINEAYEVLKDSEKRAKYDQLGKNWQAGQDFAAYGDGVHFYQSGNLNPED
                     LGGFSDFFNSIFGGQAGARSSPFTSFARQGQDVETNLELSLEELFRGGSKSIRLSSRQ
                     ICPDCQGQGVQGGGFCSRCGGTGSLPQDKNLEVKIPAGIYEGSTIRLKGQGGQGLGGG
                     PPGDLYLKVTLLPHPYFKVKGQDLEAKIVLRPEQAVLGDKITVPTMEGSVNMTIPAGS
                     HNGSRLRLKGKGLPLKEGRGDQYVVIEIDTPRDLTAEEIGLYRQLKEIGERQG"
     gene            complement(5054..6967)
                     /gene="dnaK"
                     /locus_tag="P5549_08600"
     CDS             complement(5054..6967)
                     /gene="dnaK"
                     /locus_tag="P5549_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRW13170.1"
                     /translation="MQTRTKSRRGNIMKKAVGIDLGTTNSVVAIMESGSPTVIVNAEG
                     ARTTPSVVAFKNNNERLAGQIARRQAALNPQQTFYSIKRFMGRRFSEVADERNLVPYD
                     VVAGPNDAVRFKVGEKLYAPEEISALILRKLVDDAAQYLGEPITDAVITVPAYFNDAQ
                     RQATKDAGQIAGLNVLRIINEPTAASLAYGLDKKANETILVFDLGGGTFDVSILDVGD
                     GVFEVRATNGDTHLGGDNFDKAIVDWLADEFKKDYGIDLRKDKQALQRLTEAAEKAKV
                     ELSSVSETQVSLPFITADASGPRHLDLKLTRANFENMIHDLVERCRQPVLNALQDARL
                     TASDIDEVILVGGSTRVPLVQKLVRDLIGKDPNMGVNPDEVVAVGAAIQAGVLMGEVK
                     DVVLLDVTPLSLGVETMGSLMTKLIERNTTIPARRSEIFSTAEDNQPAVDINVLQGER
                     EMARDNRSLGKFKLDGISPAPRGVPQIEVTFDIDASGILKVSARDQNTGKEQTVTISN
                     SSNLDKAEIDRMLDEAKIFTEEDKKQRAAAEAYNEADSLCYSVERQLKAMGEHLPVTD
                     KAAAENLIQEMKKAIESKASLETVEKLKNDLQSAAQRLNEASYQAGAAAGSAGADRNE
                     NEADVVDAEFEEN"
     gene            7365..8000
                     /locus_tag="P5549_08605"
     CDS             7365..8000
                     /locus_tag="P5549_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRW13171.1"
                     /translation="MENIQDYLQADENFDFEHPAIAELCRSLTEGCRDDRQKAIRLFY
                     YVRDSCHYNMYATTAEREAYQSSAILASGQGWCLQKAILLTSLARAAGIPSRLILVSI
                     RNHKSPPEAVRLMGTNLFFPHAYNHLFLNGRWVKAAATFDREICERIQVPAVEFDGEH
                     DAMLASQDLKGQPYIEYVEEYGCFATIPWPLIITNSRQIYGELSDNWFKHE"
     gene            8020..8346
                     /locus_tag="P5549_08610"
     CDS             8020..8346
                     /locus_tag="P5549_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRW13172.1"
                     /translation="MNLFRLPPVLPDEEIFEALLLDCGILIERIISCGQVTPAHQWYD
                     QERDEWVLLLQGGAKIGFEDSREQILAPGDWLLIPAHTRHRVTYTSAEPPCIWLAVHA
                     QLGMDK"
BASE COUNT         1888 a         2254 c         2107 g         2189 t
ORIGIN      
        1 aacaaaccgg tggtactgat cggcggcgga actgcctata tcggtgaccc ttccgggcgc
       61 agcgatctgc gtagcatgat gacgcccgaa atcattaaac ataacagcga gtgtttcaag
      121 caacagatgc aaaaatttat tgaattcggt gatgacaagg cgatcatggt caataatgcc
      181 gattggctgc tgcaattgaa ttatgtggaa ttgctgcggg aggtgggcaa ctgcttttcg
      241 gtaaacaata tgctgcgggc cgaatgttac aagcagcgca tggaaaaagg gctgtccttc
      301 ctggaattca actatatgat tatgcagtcc tacgactttt attatttgtt tcagcattac
      361 ggctgtaaca tgcagttcgg cggcgacgac cagtggtcca atatgcttgg cggcaccgaa
      421 cttatccgcc gcaagctggg gaaagacgcc tatgccatga cgattaccct gctgctgaca
      481 tctgaaggcg gcaagatggg gaaaaccgcc aagggggcgg tatggctcga cccggctaaa
      541 accagtcctt acgaattcta tcaatactgg cgcaacatcg acgacgcaga cgtcctcaaa
      601 tgcctgcgga tgctcacctt cctgcccctg gagcaaattg acgccatgga tcaatgggaa
      661 ggcagccagc taaacgttgc caaggaaata ttggcctatg agctcaccaa gctaattcac
      721 agccaagaag aagcggaaaa ggcccaatcc acttcccggg ctctttttgc cggaggaatc
      781 gacgtcaata tgcccaccac cgagattgcg gtcgggcaac tggagaatgg ggttatcggt
      841 atggttgaac tgatggttat gagcaagctg gcagcctcca agagcgaagc cagacgcctg
      901 atccaacagg gaggcgtatt tgttgacgac gtgaaagttg aatctgctga tgccatgatt
      961 acggcaaaac agctaaaaaa cggaataaaa atacgcaaag gcaagaaaac gtaccacaaa
     1021 atttatctga agcagcaatg ataaaaagat gagagccaac gattggcccc tcaattcaga
     1081 tttatcgcag taatgaatca tccagccgca actttaaaat gcggctggat gactgtttta
     1141 tgagccctct tggggattaa acttttaggg ttattcctgc cggagatgcg ctcagctgta
     1201 ccggactgtt ttctttgatt tctccgctga tgatcttgcg ggacagcagg gtctccactt
     1261 cctgctggat gagccgcttc agaggccggg caccgtaagc gggatggtag cctttttccg
     1321 ccagatactg cagggccttt tcatcccact gcaactgccc gcccaggttt ttttcaaagc
     1381 gccgggccag ggtctgcagc agcagatcgg taattttttc aatttgttct gtactgagcg
     1441 cctgaaatac gatgatttcg tccagacggt tcaggaactc ggggcggaaa tactgtttga
     1501 gaatgcttat tactgcttcc ttcatcaaat cataacggcc gccctgctcc tggaattgca
     1561 ggatctcctg actgcccagg ttcgaggtca taatgataat ggtgttgcga aaatcaaccg
     1621 tccgtccctg tccgtcagtc agacgcccat catccaggat ttgcagcagg atattgaaaa
     1681 cgtcattgtg agccttttca atctcatcaa acagcacgac tgaataaggg cggcgtctta
     1741 ccgcttcggt caattgtccg ccttcatcat agcccacgta tccggggggt gaaccgatca
     1801 accgggcgac gctgtgtttt tccatatact ccgacatatc catgcggatc atggcccgtt
     1861 cgtcatcaaa caaggactcg gccagggctt tggccagttc ggtcttgccg accccggtag
     1921 ggcccaggaa gataaaactg cctaccggac ggttgggatc cttcagcccg ctgcgggaac
     1981 gcaagacggc atcggccacc gcttgtacgg cctcatcctg tccgaccacc cgctggtgca
     2041 atatttcatc caaatgcatg agcttctgcc tttcaccttc cagcagccgg cttacgttga
     2101 tgccggtcca gcggctgacc actttggcaa tgtcctcttc cccgacttct tcctgcaaca
     2161 gcatctgctg accctgtttg tcagctaaag tcgcttcttc ggcctgcagt tttctttcca
     2221 gttcattcat gcggccgtat ttaagttcag cggccttgtc cagatcatag tcgcgttctg
     2281 ctttttcgat ttccatgcgg cagtcttcaa tgtcacgctt gagctggctg actcgggcga
     2341 tggcattttt ttcagattgc cacttggctt gcatggcgtc cgcttcagtc tgcagatctt
     2401 gtttttcctt tacaatatgc tcccgtcttt cccaggaagc agtatcgttt tccttgccca
     2461 gggcagtggc ctcaatttcc agctgcataa tgcgccgggt gatttcatcc agttcggttg
     2521 gcatgctgtc catttccgtg cgcagccggg cggcagcttc atccatcagg tcgatggcct
     2581 tgtcgggcag gaaacggtcg gcgatataac gatccgataa gacggctgcc gcgaccagcg
     2641 ccgcatcttt gatgcgtacc ccgtgatgga cttcgtagcg ttctttcaaa ccacgcaaaa
     2701 tggaaatggt gtcctccacc gaaggcggat tgaccaagac cggctgaaag cgcctttcca
     2761 gcgcggcatc tttttcaata tgtttacgat attcgtccag cgtggtcgca ccgatagccc
     2821 gcagttcccc ccgcgccagc atgggtttca agagattgct ggcatccatg gcgccttcgg
     2881 ctgccccggc gccgatgacg gtatgcagct catcaatgaa caggatgatt ttgccatcag
     2941 actgctcgac ttccttcagc actgccttga gccgctcttc aaattcaccg cggtatttgg
     3001 ccccggctac cagtgagccc atgtccaggg caatgacggt tttttctttt aatccctccg
     3061 gcacgtcgcg ggctacgatg cgccgtgcca gaccttctac gatggcggtt ttgcccacgc
     3121 ccggctcccc gatcagcacc ggattgttct tggtacgacg cgacaggatt tccatgaccc
     3181 ggcggatttc ttcatcccgg ccgatgaccg gatccagctt gccctgccgg gccagttcgt
     3241 ttaaatcccg cccgtagcgc tgcagtgctt cataggagtc ttcgggattg tcactgctga
     3301 cccgctgcga gccacgaata ttcttaaggg cgaccagcag ggaattacgg ttaatgccca
     3361 aacgggccag caagtcgcgc aggtcgcttt ccccttcctg aatcagaccc agcagcaggt
     3421 gttcgacact tatatattcg tctttcaaag cggcggcttc ctgctccgcc ttgcttaaaa
     3481 cccgggccag actgccgctc aggtacacct gcccttcgta accatggata gaagggagtt
     3541 tgtcaagcag atcctgcagt tgtttgctca accctggcag ttgtacgcca gccttttcca
     3601 gcagattggg cgtcagtcct ccgtcctgct gtaaaagtgc ttgcagcaga tgtttgccgc
     3661 tcacctcctg atgatgccgg gatgccgcca gttgctgggc ggtacccagg gcttcctgtg
     3721 attttttcgt ataacgctct atgttcatgg gaattacctc cgttttaacg attggatttc
     3781 ggcttccagc gcatcgatcc gatccagcag atcaagaata atggctgcgc cgctgacatt
     3841 tacgcccagc caatttctca gccgcaggag acggttgact tttttcagtt cactgcagct
     3901 tatattgtca tgttttaagg ttatgatccc catttccgcc agcattctta gaaaatcagg
     3961 actgtattcg agacgggaaa ggggaatcag ttcatcactg ttttcataat aaatcttgat
     4021 taaggtcatc gtttatccct gcctttcccc tatttctttc agttggcgat aaagtccaat
     4081 ttcctccgcg gtaagatccc ggggagtatc aatttcaatt accacatact ggtcgccccg
     4141 gccttcttta agcggcaggc ccttgccttt gagacgcaat cgggagccat tgtgactgcc
     4201 ggcagggatg gtcatgttca cgctgccttc catggtgggt acagttattt tatcgcccaa
     4261 tacggcttgt tccggacgca agacaatttt ggcttccaag tcctgcccct tgaccttgaa
     4321 ataagggtga ggcagcagag tgaccttgag ataaagatca ccgggggggc ctcctcccag
     4381 tccctgtccg ccctgacctt tcaggcggat ggtgctgcct tcgtagatgc cggcggggat
     4441 tttgacctcc aggttcttat cctgcggcag actgccggtg ccgccgcagc gactgcaaaa
     4501 tccgcctccc tgcacgccct ggccctgaca gtccggacaa atctgtcggg atgacagacg
     4561 tatcgatttg ctgccacccc gaaacaattc ttccagactg agttccagat tggtttcaac
     4621 atcctgaccc tggcgcgcaa aagaagtaaa aggtgaactg cgtgccccgg cctgaccgcc
     4681 aaatatgctg ttgaaaaaat cactgaagcc gcccaggtct tcggggttga gattgccact
     4741 ttgataaaaa tgaacgccgt cgccataggc ggcaaagtcc tgtccggcct gccagttttt
     4801 ccccaactga tcatatttgg ctcgtttttc gctgtctttc agcacttcat aagcttcatt
     4861 gatgcgcttg atcttttctt cagcttcaac ttttttcttc cccgtgtgca gatccggatg
     4921 ccattgacgg gccagcttgc ggtaggcagc cttgatatcc ttggcactgg catcacgggg
     4981 aacgcctaat atttcataat aatcctgata tgatactgcc ataactcacc tgctccgttc
     5041 ttatttcagg atgctaattt tcttcaaact cggcatccac tacatccgct tcgttttcat
     5101 tacggtctgc cccggcactg cctgccgcag caccggcttg ataactggct tcattcaggc
     5161 gttgggcagc gctttgtaaa tcgtttttga gcttttcaac cgtctccaga gatgctttgg
     5221 attcaatcgc ctttttcatc tcctggatca ggttttcggc tgccgccttg tccgtcaccg
     5281 gcaggtgttc gcccatggcc ttgagctgac gctccacact gtagcagaga ctgtcggctt
     5341 cgttataggc ttcggcagca gcccgttgtt ttttatcttc ttctgtgaat attttggctt
     5401 cgtccagcat ccgatcgatt tctgccttgt ccagattgga ggaattgctg atggtgaccg
     5461 tctgctcctt gccggtgttc tgatcccgcg cgctgacttt taaaatgccg ctggcatcga
     5521 tgtcaaaggt aacttcgatt tggggaacgc cgcgcggagc gggactgatc ccatctaatt
     5581 tgaatttacc cagggagcgg ttgtctcttg ccatctcccg ttctccctgc aatacattta
     5641 tatcgacggc gggctgattg tcctcagcgg tggaaaagat ctcgctgcgc cgcgccggaa
     5701 tggttgtatt gcgttcgatc agtttggtca tcaaactgcc catggtttca acgcccaggg
     5761 acagaggggt tacatccagc aataccacgt ccttgacttc tcccatcagc acgccggcct
     5821 gaatagctgc gccgacagcc accacttcat cgggattgac acccatgttg gggtccttgc
     5881 caattagatc gcgtaccagt ttttgcacca agggcacacg ggtcgagccc cccaccagga
     5941 tgacttcatc aatatcactt gcggtcagac gggcatcctg cagtgcattt agcactggct
     6001 gccggcatct ttccaccagg tcgtggatca tgttttcaaa attggcccgg gtcagtttga
     6061 gatccagatg gcgcgggccg ctggcatccg ctgtgataaa gggcagacta acctgtgtct
     6121 cgctcacgct ggagagttca actttggcct tttccgctgc ttcggtgagc cgctgcaggg
     6181 cttgtttgtc tttgcgcaga tcgattccat agtctttttt aaactcatcc gccagccagt
     6241 cgacgatagc tttatcgaaa ttatcaccgc ccagatgggt atcgccgttg gtggcacgga
     6301 cttcaaacac tccatctccc acatccagga tggaaacgtc aaaggttccg ccgcccagat
     6361 caaagaccag aatggtttca ttggcttttt tatccagccc gtaggccagc gaagcagcag
     6421 tcggttcatt aataatccgt aacacattga gcccggcaat ctggccggca tctttggtcg
     6481 cctgccgctg cgcgtcatta aaataagccg gaacagtgat gactgcatcg gtgatgggtt
     6541 ctcccaaata ctgggcagca tcatccacca gtttgcgcag gatcagtgcc gagatctcct
     6601 cgggggcata caatttttca cccactttaa agcgcactgc atcattgggg ccggctacca
     6661 catcataagg aaccaggttg cgttcatcgg ctacttcgga gaaacgacgt cccataaaac
     6721 gtttgatgga ataaaaagtt tgctgcggat tcaaagccgc ctggcggcgg gcaatctgcc
     6781 cggccaatct ttcgttgttg tttttaaacg cgactaccga gggcgttgta cgtgctcctt
     6841 cggcgttgac aatcacggtt ggacttcccg attccataat ggccacgacc gagttggtcg
     6901 tacccaaatc aatacctaca gctttcttca ttatatttcc cctccttgat ttggttcttg
     6961 tttgcatttg caatactgct gtaatcgtgg tcaaccagac cataaaatgg acccaaatgg
     7021 tgcgcctttg aagcagcgtt tgtttgccaa ggttaaccaa agtcaaatac acttgacaaa
     7081 ccccatttct cttgttcgga atggtaactg gtgtttctat gattagtata atcagctctg
     7141 atataatagt caataatagt caaacgattt tcggaaaaat ttttctttcc ccaggatatc
     7201 ctttaaaatg ggaaaataac gataagaacc gaagctgcca tgtggagagc aaagaaataa
     7261 tcgctgcgta tgatatgcta tataatattg gcttatttcg gcaatcaacc aagtcaatca
     7321 accaaaaacc gtgagaaaat gaaaccgaag ggaagtgcct tgctatggag aatatacagg
     7381 actatttaca ggctgacgaa aattttgact ttgagcatcc agctattgca gagctttgcc
     7441 gcagtcttac cgaaggatgc agagatgatc gacaaaaagc gatccgactt ttttattatg
     7501 ttcgtgacag ttgtcattat aatatgtatg ctacaaccgc cgaaagggaa gcctatcaat
     7561 cttctgccat cctcgccagc gggcaggggt ggtgcctgca aaaggcaata ttgctgactt
     7621 cattggcacg agctgcaggg attcccagcc gtttaatcct ggtctccatt agaaatcaca
     7681 aatccccccc tgaggcggtg agactgatgg gaaccaacct gttttttccg catgcttaca
     7741 atcatctgtt tttaaacggt agatgggtca aggcggcagc tacctttgac cgggagattt
     7801 gtgagcggat tcaggtgccg gcggtggaat tcgatggcga acatgatgcc atgctggctt
     7861 cacaggatct gaagggccag ccctatattg agtatgtcga ggaatatggc tgctttgcga
     7921 cgattccctg gccattgatt atcaccaata gccggcaaat ttacggggaa ttgagcgaca
     7981 actggtttaa gcatgaataa gggcggggcg agcgatacga tgaatctttt tagattgcca
     8041 ccggttctgc cggatgagga gatttttgaa gcactcctcc tggattgcgg tatactgatt
     8101 gaaaggatca tctcttgcgg gcaggttacg cctgcccatc aatggtatga tcaggagagg
     8161 gatgaatggg tattgctgct gcagggcggc gccaaaatcg gctttgagga tagccgggag
     8221 caaatcctgg ccccgggcga ctggctgctg attcccgccc atacacgaca ccgggttaca
     8281 tataccagtg ctgagccgcc atgtatctgg ctggcggtcc atgcccagct gggcatggac
     8341 aaatagcctt gcatgggcgt cacaacctct gcttgggggc gtctgcagcc cagtgataat
     8401 ttaactggca gtttgattaa taacacaagg agcaatga
//
LOCUS       DAOTHZ010000079         8427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_10134_length_8427_cov_7.234711, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000079 DAOTHZ010000000
VERSION     DAOTHZ010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10134_length_8427_cov_7.234711"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1413
                     /locus_tag="P5549_08615"
     CDS             43..1413
                     /locus_tag="P5549_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13173.1"
                     /translation="MSYLKEYKEKLTTAEAVAADVPSHSMVKYGYFNSKPQMLDRALA
                     ARHEELKDVTIMCAGTAPPVPEVIQHKDAFVYQDWHWTALTRILRNYYDNIIYSPVMY
                     HIVEKFVSAGEVHNFQSARPENWGWIQVGPMDEHGYFNFGPTNSENLTCMRTVGKVAV
                     EVVPSMPRCLGGQCEAIHISEVDAIIEATDEQKIFAAPDNMPGPSEVEMQIAENILPY
                     MKDGCCIQLGIGGLPNALAACIAQSDLKDLGAHTEMLVDGYVDMIESGQMNGRKKQID
                     KGKAVYTFAIGTQRMYDWMNNNPQVASYNVEYTNDPRRIAANDNFISINQAMQIDMFS
                     NVNAESMGTNHITGNGGMLDYTLGAQWSNGGCSFVCLPSTHKGKDGQLYSRIVGNFEY
                     GTNPTLTRHMIDYIVTEYGVKKMRAQNIWVRAENMIELAHPQFRDGLVEEANKLNIWT
                     RTNKID"
     gene            1939..3267
                     /locus_tag="P5549_08620"
     CDS             1939..3267
                     /locus_tag="P5549_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRW13174.1"
                     /translation="MGPLLLQLTLILVNAFFACAEIAVISMNDNKMARMAAIGDKRAI
                     RLEDLTKQPARFLSTIQVGITLAGFLASAFAAENFSDYLVNWLIGLGVTVAAPTLNTL
                     AVVFITLILSYFTLVLGELVPKRIAMRHAEKLALALSGTLYFIAQLFHPVVWLLTHST
                     NGVLRLLGIDPNAEDDVITEEEIRMMVDEGSEKGAITYNEKDMIQRIFEFNDKTAEDI
                     MTHRTEVSVLWLDESVEQWEETIFMSRHSVYPVCDESADNIVGILKVKDFIRIADRTR
                     DNLLDQALRPAYFVPEAVRADVLFHNMKNSRNHFAIVLDEYGGMSGIITMNDLLEEIV
                     GDLEDDDLVPAEKPLIEAIDSQTWKISGAAPLETVAMQLRVELPENDYDTFGGFVFGL
                     LGSIPEDGSTPELEEYGLQIKVTEIKNHRLHTAVVCLTKQAAPAELNGEK"
     gene            complement(3349..3750)
                     /locus_tag="P5549_08625"
     CDS             complement(3349..3750)
                     /locus_tag="P5549_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13175.1"
                     /translation="MKKEISGSEWVAFQVRLIFKNTRAIDLDKMAESTRNSMFDLLHI
                     HVKTVEIKRYGLLMAGKGPLQDILRICEKFYTMTNEGGLEIVLHIGGEPTFYMKEGRI
                     HNLMKDDCHDIVIPVFDDAEPDESEFDRLGI"
     gene            complement(3925..5373)
                     /locus_tag="P5549_08630"
     CDS             complement(3925..5373)
                     /locus_tag="P5549_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW13176.1"
                     /translation="MDILFIDPPYCSLKGIPTDVGYNIGLTSLAAYLRREGFASAVVM
                     GDLLMELPFMSAWGWFSVNLSQYAQGQRQYRAIVDDRNHPIWQKIGAIVREQHPQAVG
                     IPYLTPFRHSVERVAAVVKAVDPNIKVIAGAAHPTLCPQEVMQNQDIDFVVCGEGEIP
                     MAGIAAQLNRDKPDWRSVPGIYYREPGGQVTNNPPPPLIANLDDLPFVAREAVLGCDY
                     DHYRVHCLTTSRGCPYTCAFCSDRKLWSGKVRRRSVENVIAELKYLQQNYQVDVVDFS
                     DGTFTYDRKYVESFCKAIIDNHITLKWRCTARYDNLDAELLELMKNANCRGLYLGAES
                     GSDRILDSMNKRITADQIKKISQMVHDAGLDTVTAIIIGFPEETREDIEATLALMRNL
                     KTDVFDVSSFIPLPGTPAWELVADADKNEIDWRKVSYKSFDNYFLKSVSAEEFRRYMQ
                     EAYKIAGRTQRHTVIRVGLRMLRESVGSWFKS"
     gene            5834..7459
                     /locus_tag="P5549_08635"
     CDS             5834..7459
                     /locus_tag="P5549_08635"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW13177.1"
                     /translation="MSSTLIVALLFFTVGTVVLVSGIFTLQGNYKAPANRAFFALTAA
                     ITIWSSGMALSVVANDTATGEAFRRIAAIGWGTAYAFLLHFILIITGKFPSLKKRWFY
                     VCLYLPALFSLFAFAVPNTMNPFPYDLRQTAYGWINVAQHNLWDWFFYAYYIGFTLLG
                     LLLLYRWGKNSSDPLTKKKSRIVSLSIITAIVLGTITDVLLSSLFSELPQMAPVIMMI
                     PILSIYHVLQNESFGITEGVDKKTSYIIIFTSVLIYIILSALQVFLSKNLFVPVILDE
                     SAIRGIVVQIQMFISIYLVLKENRPGYITSVLMNFISLISAIAYLMRYESSESLPGII
                     SYLGVLLIITLIKAYKEKNAAYIKRINTQAVREEFYSNVFKQAPVGIAIMNDKNHTIN
                     AEFEDININPTYAKIMGRTKDELQNITWTEITHPEDLDADMVYYQQFMNGEIDQYSME
                     KRFINPDGSNIWVNMLIAPFSTSGEKSDNHLCIITDITKRKEIESVLQYNNEHVLLTG
                     LYNRSVLEKVLTRDALLPSSNKRALVCCLCERL"
     gene            7446..>8427
                     /locus_tag="P5549_08640"
     CDS             7446..>8427
                     /locus_tag="P5549_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRW13178.1"
                     /translation="MKGYENDKDLTDFCEKVSDTLRSYLYVHGINVGIGILQINKADA
                     VDTDELLKKLMNTSEAAARNNRNSPHILFYSPALDIQITRENEISQEITEISEGIKTD
                     RLHLQFQPIFNIAANRISGFEALARLNSEKHGLVLPLEFISVAEKTNMIASLGEIIII
                     QTLRFLNKLKERGYDTITVSVNISTVQMLENGFANKLLNMINDLQLSPESVGIELTES
                     VFAIKRNEIRTVIDTLKSAGIKVLIDDFGTGYSSFARVSELNIDFLKIDKSFIDKLLE
                     PNPKEAITGDIISMAHKLGHRVVAEGVEHKNQLFYLREYGCDRIQGYLISK"
BASE COUNT         2305 a         1981 c         1918 g         2223 t
ORIGIN      
        1 tattagaaat tacacttatt aaataatgaa gaggtgtaaa gtatgagtta tttgaaagag
       61 tacaaagaaa aattgaccac cgcagaggct gttgcggcag acgttccctc ccatagcatg
      121 gtcaagtacg gctatttcaa ctccaaacca caaatgctcg acagggcgtt ggcagccaga
      181 cacgaagaac ttaaagatgt caccatcatg tgcgccggta ccgcacctcc agtcccggaa
      241 gtcattcagc ataaagatgc ttttgtctac caggattggc actggacggc actgacgaga
      301 attctgcgta attactatga caacatcatt tattcaccgg tcatgtatca tatcgtggaa
      361 aaattcgtca gcgcggggga agttcacaac ttccagtcag cccggcctga aaactgggga
      421 tggatccagg ttggtcccat ggatgaacac ggctacttta actttggacc taccaattcc
      481 gagaatttga cctgcatgcg tacagtcggc aaggtggcag tggaggtcgt acccagcatg
      541 ccgcgctgcc ttgggggaca atgtgaagcc attcatattt ctgaagtaga cgcgattatc
      601 gaagcaaccg acgagcaaaa aatctttgct gcacctgata atatgcccgg cccgtctgaa
      661 gtggagatgc aaattgcgga aaacattctt ccctacatga aagacggctg ctgtatccag
      721 ttaggcatcg gcggcctgcc caatgcgctg gcagcctgca ttgctcaaag cgatctcaag
      781 gatttgggtg cgcatacgga gatgctggtt gacggatatg tagacatgat cgaatccgga
      841 caaatgaacg gacggaaaaa acaaattgac aaaggcaagg ctgtttacac ttttgccatc
      901 gggacccagc gtatgtatga ctggatgaac aacaacccgc aggtggccag ctataatgtt
      961 gaatacacca atgacccccg cagaatcgca gccaacgaca acttcatcag catcaaccag
     1021 gccatgcaga tcgatatgtt cagcaatgtc aatgccgaga gcatgggtac caatcatatc
     1081 accggcaatg gcggcatgct ggactatacc ctgggcgcac aatggtccaa cggcggctgt
     1141 agtttcgtct gtctgccctc cacccacaaa ggcaaggatg gacagctcta ctcccgcatc
     1201 gtcggcaact tcgaatacgg aaccaacccc accttgaccc gtcatatgat cgactatatc
     1261 gtaaccgaat acggcgtcaa gaaaatgcgc gcccagaata tttgggtcag agccgaaaat
     1321 atgatcgaac tggcccatcc ccaattcaga gatggcttgg tggaagaagc caacaagctg
     1381 aacatctgga cccggaccaa caagatcgat tagtcacacc ctatcatcaa atgcattcgc
     1441 ccaggcagcc cggcactgtt cgcagatacc gggctgcttt tgtgtttagc cttgcgtttt
     1501 cagttgcctt aagtcccacg tttgtcaccg gttccgggat atgccaagcc aagccaactc
     1561 atcaagacat tccagcaata acggcagcgc actatccttc tgcggttacc aaacaatctc
     1621 atctggtccc ctgttttagg taaagacagc tcacaggcag cttaatcata agtggtcctt
     1681 gctttgacag cccggtgaat atatggtatc attttcccaa tttataaagg agtatgctga
     1741 attggacgat tcaccttacc cgcaacgaag acgctggaag ttaatattga gatccaacca
     1801 cgcgacgctt tcccaggcag ataaacggat cggtctgaga gcaggcggcg ctttttttca
     1861 tgttttgccg gaaagtgatc ttggtttgct tgggtttaca catggggagg aattatcttg
     1921 gaaggaataa acagcatcat gggaccgctt ctgctgcaat tgactctaat actggtcaat
     1981 gctttctttg cttgtgcgga aatcgcagtc atttccatga acgacaataa aatggcacgt
     2041 atggcggcca taggcgacaa acgcgccatc cgcctggaag atttgaccaa acagcctgcc
     2101 cgctttctct cgacgattca ggtgggcatc accctggcag ggttcctggc cagtgccttc
     2161 gccgccgaga atttttccga ttatctggtc aactggctga tcggcttggg agtaacggtt
     2221 gcagcgccaa cgctgaacac actggcggtg gtcttcatca ccttgattct atcctatttt
     2281 acactggtgc tgggggaact ggtccccaaa cgtattgcca tgcgccatgc ggaaaaactg
     2341 gccttggcat tgtcgggtac gctgtacttt atcgcccagc tgtttcatcc agtggtttgg
     2401 ctgcttaccc actccaccaa cggcgtgctg cgcctgctgg gcattgatcc caacgccgaa
     2461 gatgatgtga tcaccgaaga agagattcgc atgatggtgg atgaaggcag tgaaaaaggg
     2521 gctatcactt acaacgaaaa agacatgatt cagcggattt ttgaattcaa tgacaaaacc
     2581 gccgaagaca tcatgaccca tcgcaccgaa gtttccgtcc tgtggttgga tgaaagcgtt
     2641 gaacagtggg aggaaaccat tttcatgagc cggcactcgg tgtacccggt ttgtgatgaa
     2701 agtgctgaca acatcgtcgg catcctgaag gtaaaggatt ttatccgtat tgctgacaga
     2761 actcgcgaca atctcctgga tcaggcgctg cggcctgcct atttcgtacc ggaggccgtt
     2821 cgtgctgatg tattgttcca taacatgaaa aacagccgca accactttgc cattgttctg
     2881 gacgaatacg ggggtatgag cggcatcatt accatgaacg atttgctgga ggaaattgtg
     2941 ggggatttgg aagatgatga tttagttcct gctgaaaagc ctttaattga agcgattgat
     3001 tcgcagacct ggaaaatctc aggtgcagca ccgctggaaa cggttgccat gcagctgcgg
     3061 gtggagctgc cggaaaatga ctatgatacg tttggcggct ttgtttttgg attattgggc
     3121 agtattcccg aagatggcag tacccctgaa ctggaagagt acggactgca gatcaaagtc
     3181 actgaaatca agaatcaccg tttgcatacc gctgtcgtct gtctgaccaa acaggcagcc
     3241 ccggcggagc ttaacggcga aaaataacgc aagcgcttga acacttgcgt tgtcgtcaat
     3301 tcatgaagga aaccgcctgg tcggcggttt ctttttatat gctatgtgtt atattcccag
     3361 tcggtcaaat tccgattcat ccggttcggc atcatcaaac accggaatca caatatcatg
     3421 gcaatcgtct ttcattagat tatggatgcg cccctccttc atatagaagg tcggttcacc
     3481 cccaatgtgc aggacaattt ccagtcctcc ctcattggtc atggtgtaaa atttctcgca
     3541 tatgcgcagg atatcctgaa gcggaccttt gccggccatt aacagcccat aacgtttgat
     3601 ctcaaccgtc ttgacatgga tatgcaaaag gtcaaacatc gaatttctgg tgctctccgc
     3661 cattttgtcc aagtcaatgg cccgggtgtt tttgaagatc agacgcacct gaaaggcaac
     3721 ccactcggaa cctgaaatct ccttcttcat gttatatccc cccattatat aatagagtat
     3781 atggttatta taagctgtcc ttgatcattt ggcaaaatat tttgaaattt taaccaacac
     3841 ttcgacaaat gttggttttt aaatcaagaa tctccttggt aatataaagt ttttattttt
     3901 acggggggct ggccaacgtc tgcgtcagga tttgaaccag ctaccaacgg attcccgcaa
     3961 catgcgcaat ccgactcgaa taaccgtgtg gcgctgggtt cggccggcaa ttttataggc
     4021 ttcctgcata taacggcgaa attcttcggc cgaaacactt ttcaggaaat aattatcgaa
     4081 cgacttgtag cttacctttc tccagtcaat ctcgtttttg tcggcatccg caacgagctc
     4141 ccatgccgga gtacccggca aaggaataaa actgctgaca tcaaaaacat ctgttttgag
     4201 atttctcatc aacgccaagg ttgcttcaat atcctcccgg gtttcttccg gaaaaccgat
     4261 gataattgcc gtcaccgtat ccaagcctgc atcatgcacc atctgactaa tctttttaat
     4321 ctggtccgca gtgattcttt tattcatgct atccagaatc ctgtcagatc cggattccgc
     4381 ccccaggtac aggcctcggc aattggcgtt tttcatcagt tcaagcagtt cggcgtccag
     4441 attatcatag cgtgctgtgc agcgccactt gagcgtgata tggttgtcaa ttatggcctt
     4501 gcagaatgat tccacatatt ttcggtcata ggtaaaggta ccatcagaaa aatcgaccac
     4561 atcaacttga taattctgtt gcaagtattt taattccgca attacgtttt ccacactgcg
     4621 ccgccgcact ttgccggacc ataatttccg atccgaacaa aatgcacagg tgtaaggaca
     4681 gccccgcgag gtagtcagac agtgtacgcg gtaatggtca taatcgcagc ccagtacagc
     4741 ctcccgggca acaaaaggca aatcgtccag attggcaatg agcggcggcg gcgggttgtt
     4801 cgtcacctgt ccgcccggct cgcggtaata gatccccgga accgaacgcc agtccggctt
     4861 gtcccggttt aactgggcag cgatgcctgc catcggaatc tcgccctccc cgcagaccac
     4921 aaagtcaatg tcctgattct gcatgacttc ctgggggcac aaagtaggat gggccgcccc
     4981 ggcgatcacc ttgatgttgg ggtccaccgc tttgacaacg gccgctactc tttccacaga
     5041 atgccgaaat ggtgtaagat agggaattcc aaccgcctgg gggtgttgct cccgcacaat
     5101 cgcaccgatt ttctgccaga tgggatggtt gcggtcatcc acaatggccc gatattgacg
     5161 ctgtccctgg gcgtattggc ttagattgac agaaaaccag ccccaggcac tcataaaagg
     5221 tagctccata agcaagtctc ccatcaccac ggcagacgca aaaccttctc tgcgcaaata
     5281 cgcggccaga cttgtcaagc caatgttata gcctacatca gtggggatac cctttaaact
     5341 gcaataggga ggatcaatga ataatatatc catgagcata aattccaatc ataatctatt
     5401 agacggcctc attataggcg attgtcctgc caactgtcaa gctggccaga aaagcgaacc
     5461 tcaatatatt gggatttcgt cacgatgatt gcttttcctg caacgcttgt tggtcggaat
     5521 tgaatttctc ctgtggtacc aacgagatct ttcccagatt ggctaaaatg aattcgttat
     5581 cctttggccc cacaatgagt taagtacctc agaaaaactc accctggctg aaaaatagaa
     5641 aaatcctgtt ctttctgccg gctggtcagc aggtaccccc attcgtccat atcacatatt
     5701 attttccaca aaactaacaa tcaagtctgt tttataggca taaataagac gccttgagta
     5761 tcctcttgcg gcatttttcc agtataatag acatatatga cataatctct gctgaaaacg
     5821 agaggagaat cgagtgtcca gtacattaat agtggcacta ttattcttta ctgtgggtac
     5881 ggttgtgctg gtcagtggga tcttcacatt gcaaggcaat tataaagctc cagccaacag
     5941 ggcatttttt gccctcaccg cagcgattac catttggtcg tccggcatgg cgctgtcagt
     6001 tgttgccaat gacactgcaa cgggcgaagc ttttcgtcga attgcagcca tcggctgggg
     6061 cacagcttac gcgtttttgc tccattttat cctaatcatc acgggtaaat tcccatcctt
     6121 gaaaaagcgg tggttctacg tatgcctgta tttaccagcg ctcttctctt tgtttgcttt
     6181 tgccgttcca aatacaatga accctttccc ctatgatctg cgccagacag catatggatg
     6241 gataaatgta gcacagcaca atctttggga ttggtttttt tatgcgtatt atattggttt
     6301 tacactcctc ggattattgc ttttatatcg gtggggtaaa aattcatcag atcctctcac
     6361 aaaaaagaaa tcacgcattg tgagtctatc aatcatcaca gctattgttc tgggaacaat
     6421 tactgatgtg cttttaagca gcctgttttc cgagctgccg caaatggctc cggttattat
     6481 gatgatacct attctgtcca tataccatgt tcttcaaaat gaaagctttg ggataaccga
     6541 aggggtggat aaaaaaacca gctacatcat tattttcaca agtgttctta tctacattat
     6601 actttctgcc ctgcaggtat ttctttcaaa gaatcttttt gttccagtca ttttggacga
     6661 atcggctatc agaggaatcg ttgttcaaat ccagatgttc atatcaatct accttgtttt
     6721 gaaagaaaac cgaccagggt atatcacatc tgttttgatg aatttcatca gcctcataag
     6781 tgcgatcgca tatcttatgc gatacgagtc aagtgaatct ttaccaggaa tcatatctta
     6841 tctaggcgtc cttttgatca tcacgctgat aaaagcttat aaagagaaaa atgctgctta
     6901 tataaaaaga attaacacgc aggcagtccg agaagaattc tattccaatg tgtttaagca
     6961 ggcaccggtt gggatagcga tcatgaatga caaaaaccat acaattaatg cagaatttga
     7021 agatattaat attaacccga catatgcgaa aattatgggg cggacaaagg acgaactcca
     7081 aaatattacc tggaccgaga taacccatcc ggaagacctg gatgctgata tggtctatta
     7141 ccaacaattt atgaatggcg agatcgatca gtactcgatg gaaaagcgct ttattaatcc
     7201 ggacggctct aatatttggg ttaatatgct aattgctccg ttcagtacat cgggcgaaaa
     7261 atccgataat catctctgta tcataacgga cataacaaag cgtaaggaaa ttgaatccgt
     7321 tctgcaatac aacaatgaac atgtgctgct gacaggactt tataaccgca gtgtattgga
     7381 aaaagtattg actagagacg ctttgctgcc ctcgtcaaac aagcgggcgt tggtgtgttg
     7441 cttatgtgaa aggctatgaa aacgacaaag atctgactga tttttgtgaa aaggtatctg
     7501 atacgttgcg ttcttatctt tatgtccatg gaattaatgt gggtatcgga atccttcaga
     7561 ttaataaagc tgatgcggtc gatacagatg agttattgaa aaagctcatg aatacaagtg
     7621 aagcagcggc aagaaacaat cgcaatagtc ctcatatttt attctacagc ccggcattag
     7681 atattcagat tacccgcgag aatgagatca gccaagagat aacagaaatc tcagagggta
     7741 tcaagaccga cagacttcat ttgcagtttc agccgatatt taacatcgct gccaatagga
     7801 tttccggatt cgaagcttta gcccgactaa acagtgaaaa acatggtttg gttcttcctt
     7861 tggagttcat atcggtagcc gaaaaaacaa acatgattgc ttcattggga gaaataatca
     7921 ttattcagac cctgcgtttt ttaaacaagc tgaaagaacg tggctacgat acgataactg
     7981 tatcggttaa tatttcgacg gtgcagatgc tggaaaatgg ttttgccaac aagttgctga
     8041 acatgatcaa cgatttgcaa ttgagtccgg aaagtgtagg tattgagctt actgaatcag
     8101 tattcgccat aaagagaaat gaaattagaa cggttattga cacgctcaaa tctgctggca
     8161 ttaaagtgct catcgacgat tttgggacag gttactcttc tttcgctcgt gtcagtgaat
     8221 taaacattga tttcctgaaa attgataaaa gctttattga taaattgctg gagccaaacc
     8281 cgaaagaggc gataaccgga gatatcatat ccatggctca caagttgggc caccgtgttg
     8341 tcgccgaagg cgttgagcac aagaatcaac tgttttactt acgggagtat ggttgtgaca
     8401 ggatacaggg atatctgata agcaaac
//
LOCUS       DAOTHZ010000080         8406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_10165_length_8406_cov_7.247994, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000080 DAOTHZ010000000
VERSION     DAOTHZ010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10165_length_8406_cov_7.247994"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="P5549_08645"
     CDS             <1..1276
                     /locus_tag="P5549_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HRW13179.1"
                     /translation="DWDACTGCGTCIEKCPTKKLADEFEFGMGKRTAIHKLFPQAVPG
                     KPYIDAENCLKLTKDKCGICEKVCPTDAINFQDMAVYQEIEIGAIVMATGYDQFNWQE
                     AYGEYGYGKYPDVISGLEFERLVSAGGPTGGHIVRPSDGTEPKNIAFIKCVGSRDDTK
                     GKSYCSRACCMYTAKHAYQVKTKIEDSEAYVFYMDVRTAGKNYEEFYQRALNAGAKYI
                     RGRVSKIYPRGEKLILKSEDTLMGMPIEVEADMVVLATAMVPSVGSGELAKTLGFSVD
                     KDGWFQEAHPKLQPVETFAAGVYLAGTCQGPKDIPDTVAQASGAAVKVLGLFSKNELA
                     TEPMVSEVDVSKCSGCGLCVPICPYNALSLVPYKERGGHGHGPFTRQVAEVNNSLCQG
                     CGACLPACRTLALNLKGFTNDQLVSEVDALCL"
     gene            1267..1701
                     /locus_tag="P5549_08650"
     CDS             1267..1701
                     /locus_tag="P5549_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1480..1482,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HRW13180.1"
                     /translation="MSIDTQAEWRPKIIVFACNWCSYAAADLAGLNHLEYSSDIRIIR
                     TPCSGRMDPMLVLRAFNRGVDGVLLSGUHPGDCHYGSGNYYQRRRQLVMTRLMQFIGI
                     EPERYQVAWISGAEGIKFQETMAQLVNDVKKLGPNRKLRDAR"
     gene            1698..2603
                     /locus_tag="P5549_08655"
     CDS             1698..2603
                     /locus_tag="P5549_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW13181.1"
                     /translation="MKEITNKLQEIAARLFNEGKIDVFLAWEKGEHDFQTKSYAARNV
                     DDVKNIVFNEYTIYNVSNALLKFRDSQDRIGIAVKGCDSRGIVRLLEDLQITRERLYI
                     VGIPCPGLKDPLAAARKHGGLGKKEEPESLAKKCLECIQPNPVIYDELIGPEQTPRQQ
                     GDRFAAVRELEAMTPDERYQFWEDTLSTCIRCYACRQVCVACDCRTCIFDEMKPQWVG
                     RETNTTDNMMYHLVKHAHMAGRCIDCGECERVCPVGIPLMLLNRKLIKDVAELFGGPE
                     AGMQYVEGAKPALSVYRENDPDEFI"
     gene            2615..3631
                     /locus_tag="P5549_08660"
     CDS             2615..3631
                     /locus_tag="P5549_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW13182.1"
                     /translation="MKVLRKAKLDEALNLLNEAHEVFVPMQKKAQSGYFPWSSFISGQ
                     DELMLEALNVYQSPKGVVLPQTEKMYRYRQGGMEIKIEETFEDSHPTIIFGARACDVK
                     GILAMDEVFLTRGYEDSFYKARRSKNTIIANACYEPGPNCFCQAMEVDCLNPAGADVI
                     LRDGGDAYVWEVKSEKGEAVTAAIAGLLEEKEAALPQPKPFTQKVDYSGVAEKLKTMF
                     DHPLWDKLAEPCVNCGMCTYVCPSCYCFDIQVKMWGEEGYRFRAWDSCMFEEYSREAG
                     GGNPRGASKERFRQRFLHKLQFFTERYGTPLCTGCGRCMIVCPTDVNIVRIIEAIKEA
                     EVND"
     gene            3624..4475
                     /locus_tag="P5549_08665"
     CDS             3624..4475
                     /locus_tag="P5549_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRW13183.1"
                     /translation="MTDKDHKCTCNEQPLVPKVVEVIDIIDRTPDVKSFFVKNPGDSG
                     LPFQVSPGQCAMVSMPIGEAMISCTWQEEQEYLEFAIKRVGLLTDELHQIEAGQQIGL
                     RGPYGNGFPVEACKGKDMVFIAGGIGLAPLRSFIKYCFKHREDYGKITILYGSRSQAD
                     LCFKEELFELWPQEANTEVFVTIDNPEPDWDGHVGFVPAYLEELNPNPAGAVAVVCGP
                     PIMIKFVLQSLEKMGYQDDQVITTLEMRMKCGIGKCGRCNIGSEFICLDGPVYYLAQL
                     RNLPPEW"
     gene            4620..5618
                     /locus_tag="P5549_08670"
     CDS             4620..5618
                     /locus_tag="P5549_08670"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW13184.1"
                     /translation="MKVLAKARIEKLLDRLASGAEVYVPMLRGPGTGFYTWTSYDEDY
                     DELVLDRLNVYMPPKHIMITAAEKNHINLLQDSGEVNEKIIFGIRSCDVQGIVLLDEF
                     CDAEGVSSTLFHSRRDRTIMIANACYHPAPSCFCDSVGIDPREPVGADVIIRDTGRDG
                     YVWEARSPKGERLTQKVEDLLEEKQVTVPALQPFSLQVNWDGVTEKLGHMQDHPLWEK
                     HSEACQTCALCTLTCPACNCMDWQADVWRETGYAFACRESCAYKNDTLMPESSGRRQA
                     ATERFRNRWLHKLHFYPQRYGKIQCTGCGRCIVLCPSGASISKMITEIQEADVSSG"
     gene            5602..5790
                     /locus_tag="P5549_08675"
     CDS             5602..5790
                     /locus_tag="P5549_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13185.1"
                     /translation="MCHPASTINQGFLYLEKDNNVSRETLLRNESYNIGLYINIDEVT
                     LQGRPLVFPETGLNHTRV"
     gene            complement(5791..7860)
                     /locus_tag="P5549_08680"
     CDS             complement(5791..7860)
                     /locus_tag="P5549_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRW13186.1"
                     /translation="MDTKWKRFSQATITKLIVFIFAVACFSGAITPLFDAYVTHSTDF
                     SILNEDSYYVNSDFINEITPIVNNLKKITSEYQNEDYIVSGGSLSAEEINHETEQLFE
                     EFADTSLSYNPKLSPAENQVIFQRDYADRIARIKDQLIQDDLRDYHSIMRELGQYKGL
                     VYYAKSGETVFTNSPSQSAGYFKSFPAYLTLEGYDQNVYPSDIQSSRNYGWLQSTMDE
                     FGHQDIMYLALTDQFLNPRISEWKENKTLMDNSLRQIGWLVMGFFLAFLYLLWVSGRR
                     PGEDKIYFNSLDRIYNDINLLLCTLLIIAEIGSLVALQQMKLFEFVFPVTFIIAAMGL
                     LLVLAMVKHLKNRTFLRHTLTYVVFEKLFVFVKDVYHTGSTAVRVILVVIAYPLIAGL
                     TFFMFPLTIGFACWLALKKVKEFNLLQAGVQEVKEGNLNHTIEIPGDSDFALLAADIN
                     HITDGLSQAVAKEVKSERLKSELITNVSHDLRTPLTSIITYVDLVKQETDPEKMQEYI
                     EIIDQKAQRLKLLTDDLFEAAKASSGDMPVNLEKIDIVSLITQGLGELDDKVQQQKLE
                     IKLSHPQEKMFVKADGKLLWRALENLLSNIFKYAQEGSRVYIDLEDADNEVRMTMKNI
                     SAFALNVSAEELMERFKRGDEARTSQGSGLGLSIARSLIEIQNGSFQVDIDGDLFKVT
                     LRLESLA"
     gene            complement(7835..8404)
                     /locus_tag="P5549_08685"
     CDS             complement(7835..8404)
                     /locus_tag="P5549_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW13187.1"
                     /translation="MEENPIHLVIMDIMMPKMDGMRATLKIRENNNIPVIMLSARSED
                     TDKILGLNMGADDYITKPFNPLELIARVKSQLRRYTTLGSLETKVNVFRTGGLEIDDE
                     SKCVTVDGEEVKLTPVQYKILKLLTAHAGRVFSIDEIYEKVWNENAFGVENTVAVHIR
                     KIREKIEINPKEPKYLKVVWGIGYKVEKI"
BASE COUNT         2154 a         1978 c         2183 g         2091 t
ORIGIN      
        1 cgactgggac gcctgtacag gttgcggcac ctgtattgaa aaatgcccca ccaagaaact
       61 ggcggatgaa tttgaattcg gtatgggaaa aagaactgcc attcataaac tatttcccca
      121 ggcggttccc ggcaaacctt atatcgacgc ggaaaactgc ctgaagctga ccaaagacaa
      181 atgcggcatc tgtgaaaagg tatgtcccac cgatgcgatt aattttcagg atatggcggt
      241 ttatcaggag atcgaaattg gagccattgt aatggccacc ggttacgatc aattcaattg
      301 gcaagaagca tatggcgaat acggttacgg aaagtatccg gatgtgatca gcgggctgga
      361 atttgaaaga ctggtctccg ccggcggacc gaccggcggc cacattgttc gtccttcgga
      421 cggcacagag cccaaaaaca ttgcttttat caagtgtgtc ggctctcggg atgataccaa
      481 gggcaagagc tattgttccc gtgcttgttg tatgtatacc gccaaacacg cttatcaggt
      541 caaaaccaaa atcgaagaca gtgaagctta cgttttctat atggacgtgc gtacggccgg
      601 caagaactat gaagaattct atcagcgggc actgaacgcc ggagccaaat atattcgcgg
      661 ccgggtcagc aagatttatc cgcgcggcga aaagctcatt ctgaaatcag aagataccct
      721 gatgggcatg ccgattgaag tggaagcaga catggtcgtc ctggctactg ccatggtgcc
      781 gtcggtgggc tcaggtgagc tggcaaagac actgggcttt tcagttgaca aggatggctg
      841 gttccaggaa gcccatccca aactgcagcc ggtggagacg tttgccgccg gagtttatct
      901 ggccggtacc tgtcagggtc cgaaagatat acccgacacc gtcgcccagg ccagtggtgc
      961 agctgtgaaa gtactgggct tgttctccaa gaacgaactg gcgactgaac cgatggtatc
     1021 cgaagtggat gtaagcaaat gttcgggatg cggtttgtgt gtaccgattt gcccttacaa
     1081 tgccctttcc ctggtgccgt acaaagaacg cggcggacac gggcacggac ctttcacccg
     1141 tcaagtggcg gaagtcaaca acagcctctg tcagggatgc ggtgcctgtc tgccggcttg
     1201 ccggacattg gccctgaatc tcaagggctt taccaatgac caacttgtat cggaggtgga
     1261 tgcattatgt ctatagatac acaggcagaa tggaggccca agatcatcgt gtttgcctgc
     1321 aactggtgtt cctatgcggc agcagatttg gccggcctga atcatttgga atattcatca
     1381 gatatccgca ttatccgtac cccttgttcc ggccgcatgg accccatgct ggtcttgcgg
     1441 gctttcaacc ggggcgtgga cggggtgctg ctctcggggt gacacccggg tgactgccac
     1501 tatggtagtg gcaattacta tcagcgccgg cgccagctgg tgatgaccag actgatgcag
     1561 tttataggca ttgaaccgga aagatatcag gtggcctgga tttccggtgc agaagggatc
     1621 aagtttcagg agaccatggc ccaactggtc aatgatgtta aaaagctggg acccaataga
     1681 aagctgaggg atgcgcgatg aaagagataa cgaacaaact gcaggaaatt gcggcccggc
     1741 ttttcaacga gggaaaaatt gacgtatttc tggcttggga aaaaggagag cacgatttcc
     1801 agaccaaatc ctatgcggca cgaaatgttg atgatgtgaa aaacatcgtt ttcaatgaat
     1861 atacgattta taacgtttcc aatgcattgt taaaattccg ggacagtcag gatcggatcg
     1921 gcatcgcggt caaaggatgc gattcccggg ggattgtcag actgctggaa gacctgcaga
     1981 tcacccgtga gcgcctctac atcgtcggca ttccctgccc gggattgaaa gatccgctgg
     2041 ctgccgcgag aaaacacggc ggattaggca agaaagaaga acccgaaagc ctggcgaaaa
     2101 aatgcttgga atgtatccag cccaatcccg tcatctatga tgaactgatt ggacctgaac
     2161 agacgccccg gcaacaaggc gaccgttttg ctgcagtacg ggaactggag gcgatgactc
     2221 ccgatgaacg ctatcagttc tgggaagaca cgttgtccac ctgtatccgt tgttatgcct
     2281 gccgtcaggt atgtgtagcc tgtgactgcc gcacctgtat ctttgatgaa atgaaacctc
     2341 aatgggtggg acgggaaacc aataccactg acaacatgat gtatcatttg gtcaagcacg
     2401 cccatatggc agggcgctgc atcgactgcg gcgaatgtga aagagtctgc ccggtaggaa
     2461 ttccgctcat gctgctgaat cgcaaactga tcaaggatgt ggcggaactg tttggcgggc
     2521 cggaagccgg catgcaatac gtggaaggtg ccaaaccggc cctgagcgtt taccgggaaa
     2581 atgacccgga cgaattcata tagggggtga aaatgtgaag gtgttacgca aagccaaact
     2641 ggatgaagcg ctcaatctat taaatgaagc ccatgaagtg tttgtgccca tgcaaaagaa
     2701 agcccagagc ggctattttc catggagcag ttttatatcc ggtcaggacg aattgatgct
     2761 ggaagccctg aacgtttatc agtcccccaa aggtgtggta ctgccccaaa ccgaaaaaat
     2821 gtaccgctat cgacagggcg gcatggaaat aaagattgaa gagacttttg aggattccca
     2881 tccgacgatt atctttggcg cccgggcctg cgatgtgaaa ggcattctgg ccatggatga
     2941 agtatttttg acccgcgggt atgaagacag tttttacaaa gcgcgccgga gcaaaaatac
     3001 catcattgcc aatgcctgtt acgaacccgg tcccaattgc ttttgccagg ccatggaagt
     3061 ggactgcctc aatcctgccg gtgcggatgt catcctgcgt gacggaggag atgcatacgt
     3121 ctgggaagta aaaagcgaaa aaggggaagc ggttaccgct gccatcgccg gattgctgga
     3181 agaaaaagaa gcagcgttgc cgcagccaaa gccatttacg caaaaagtgg attacagcgg
     3241 cgtggcagag aaactcaaga ccatgtttga tcatccgctg tgggataagc tggcagaacc
     3301 ttgcgtgaac tgtgggatgt gtacgtatgt ttgtccgtct tgttattgtt ttgatattca
     3361 ggtgaaaatg tggggtgaag aaggttatcg ctttcgggcc tgggattcct gcatgtttga
     3421 ggagtacagc cgcgaagccg gcggaggcaa cccgagaggc gccagcaaag aacgttttcg
     3481 tcagcgtttc ctgcacaagc tgcaattctt taccgaaaga tatggaacgc ctttgtgtac
     3541 cggttgcgga agatgcatga tcgtctgccc gacggatgtg aacatcgtga gaatcatcga
     3601 ggccataaag gaggcagagg tcaatgactg ataaagatca caagtgtacc tgcaatgagc
     3661 agcccctggt acccaaggtg gttgaggtaa tcgatatcat cgaccgtacc ccggatgtaa
     3721 aaagcttttt cgtgaaaaat ccgggtgaca gcggtttgcc tttccaggta tcccccggtc
     3781 aatgtgccat ggtctccatg cccattggcg aagccatgat ttcttgcacc tggcaggaag
     3841 aacaggaata cctggaattt gctatcaaac gagttggctt gctgaccgat gaactgcacc
     3901 agattgaagc cggacagcaa atcggtctgc gcggacctta tggcaacggc tttccggtgg
     3961 aagcctgcaa aggtaaagac atggtcttta ttgccggggg gatcgggctg gcgccgctgc
     4021 gttcgtttat caagtattgt ttcaaacatc gtgaagatta cggtaagatt accatcctgt
     4081 acggatcgcg cagtcaggcc gacctttgtt ttaaagaaga gctgtttgag ctgtggcccc
     4141 aggaagccaa taccgaagta ttcgtaacca ttgacaatcc tgagccggat tgggacggtc
     4201 atgtgggttt tgtgccggcc tatctggaag aacttaaccc caatcctgct ggtgccgtgg
     4261 cagtcgtttg cgggccgccc atcatgatca agtttgtctt gcaatcgctg gaaaaaatgg
     4321 gctatcagga cgatcaggtc attaccaccc tggaaatgcg gatgaaatgc ggaatcggca
     4381 aatgcggccg ctgcaacatc ggcagcgaat tcatctgtct ggacggtccg gtatattatc
     4441 ttgcccaatt acgcaatctg cccccggaat ggtaaaaata atatataata aaagctcagg
     4501 gtcaagcaca tgctcttgcc ctgagctttt ttgcggatgg agaaacgctt cgcctgcagc
     4561 aagggagaag cagagcaatg aaaatatttt tgattatacg attagtttga ggtgagaaga
     4621 tgaaagtatt ggcaaaagcc aggattgaaa agctgctgga cagattggcg agtggtgcag
     4681 aagtgtatgt gcccatgctg cgcggtcccg gaacgggttt ttatacatgg acgtcttatg
     4741 acgaggacta tgatgaactg gtgctcgatc ggctgaatgt atatatgccg cccaagcaca
     4801 tcatgattac tgctgccgaa aagaaccaca taaatttatt acaggacagc ggggaagtaa
     4861 acgaaaaaat tatttttggc atccgcagct gtgacgtaca ggggattgta ttgctggatg
     4921 agttctgtga cgcggaaggg gtttcctcta cattatttca ctcccgccgt gatcgcacca
     4981 taatgattgc caatgcctgt taccatcctg cccccagttg cttttgcgat tcggtgggga
     5041 ttgacccgcg tgaacctgtc ggggcggatg ttatcattcg cgatacaggc cgcgatggtt
     5101 atgtctggga ggcgcgctcg cccaagggag aacgattgac gcaaaaagtg gaagatctgc
     5161 tggaagaaaa acaagtcaca gttcctgcat tacaaccttt ttccctgcag gtaaactggg
     5221 atggagtcac tgaaaaatta gggcatatgc aggatcatcc gctttgggaa aaacactcgg
     5281 aagcatgtca gacctgtgcg ctttgtacct tgacctgtcc tgcctgcaat tgcatggact
     5341 ggcaggcgga tgtctggcgg gaaacaggct atgcatttgc ctgtcgggaa agctgtgctt
     5401 ataaaaacga taccctgatg ccagaatcct ccggtcggcg gcaagctgcc acggaaagat
     5461 tccgcaaccg ctggctgcac aaactgcact tttatcccca gcgttatggc aagatacaat
     5521 gcaccggctg cggacgctgt atcgttcttt gtccctccgg cgccagtatc agcaagatga
     5581 ttacagaaat tcaggaggct gatgtgtcat ccggctagca ccataaatca gggatttttg
     5641 tatttggaaa aagacaacaa tgtttcacgt gaaacattgt tgcgaaatga atcctataat
     5701 attgggcttt atataaacat tgatgaagtg accttgcaag gccggcctct ggttttcccg
     5761 gagaccggcc tcaatcatac ccgtgtttga ctatgccaac gattccagac ggagcgtgac
     5821 tttgaacaaa tccccgtcga tatcgacctg aaaacttcca ttttgaatct cgatcagact
     5881 tcgggcaatc gataatccca atccgctgcc ttgactggtt ctggcttcat cgccgcgttt
     5941 aaagcgttcc atcagctctt cggcggacac gttgagcgca aaggccgaga tgtttttcat
     6001 cgtcatgcgc acttcgttgt cagcgtcttc cagatcaatg tatacccgtg atccttcctg
     6061 ggcgtattta aagatattgg acagcaaatt ctccagagct ctccataata atttgccgtc
     6121 tgctttgacg aacatttttt cctggggatg actgagcttg atttccagct tttgttgctg
     6181 caccttgtca tccagttccc ccaatccctg ggtaattaaa gagacaatat caattttttc
     6241 aaggttgaca ggcatatccc cgctggaagc cttggccgct tcaaacaaat catctgtcag
     6301 gagttttaat cgctgggcct tctggtcgat gatttcgatg tattcctgca tcttttccgg
     6361 atccgtttcc tgcttcacca gatccacata cgtaatgatg gaagtcagag gggttcgcag
     6421 atcatgcgat acattggtaa ttaactcgga tttaagacgt tcgcttttga cttctttggc
     6481 gacggcctgg ctcaatccat cggtaatatg attgatatct gctgccagta gggcaaaatc
     6541 actgtcacct gggatctcga tggtgtgatt gaggttacct tccttgactt cttgcacacc
     6601 ggcctggagg agattgaatt ccttgacttt tttcaaggcc agccagcagg caaaaccaat
     6661 cgtcagcgga aacataaaga acgtcagtcc ggcaatgagg ggataggcga tcacgaccaa
     6721 aatgactctt acggctgtcg aaccggtatg atagacgtct ttgacaaaga cgaacaattt
     6781 ttcaaaaacg acataggtca gggtatggcg cagaaaagtc ctgttcttca ggtgcttaac
     6841 catggccagg accagcagca gacccatggc ggcgatgata aaggtgaccg ggaaaacaaa
     6901 ttcgaagagc ttcatttgct gcaatgctac cagcgaacct atttcagcaa taatcagcag
     6961 ggtgcacagc aacagattta tatcattgta aatccgatcc aaggaattga aataaatctt
     7021 gtcttccccg ggacgccgac cgctgaccca caacagataa agaaaggcca ggaaaaatcc
     7081 catgaccagc cagccgattt gccgcaaact gttatccata agggttttgt tctctttcca
     7141 ttcggatata cggggattta aaaattgatc agtcaaagcc agatacatga tatcctgatg
     7201 accgaactca tccatggtcg actggagcca gccgtaattt ctgctggact ggatgtctga
     7261 cggatataca ttctgatcgt atccttccaa agtcagatag gcagggaatg acttgaaata
     7321 gcctgcgctc tggctgggac tgttggtgaa tactgtctcg cccgattttg cataataaac
     7381 cagccctttg tattgtccca gctcgcgcat gatggaatga taatcccgca ggtcatcctg
     7441 gattaactga tccttgatcc gggcaatccg atccgcgtag tcacgctgaa agatgacctg
     7501 gttttcggcc ggactcaatt tggggttata actgagggaa gtgtcagcaa attcctcaaa
     7561 cagctgctcg gtttcgtgat tgatttcctc tgcgctgaga ctgccgccgc ttacaatgta
     7621 atcctcattt tggtattcgg aggtgatttt tttcaggttg ttgacaatcg gagtaatttc
     7681 attgataaaa tcactgttta cataatagct atcttcattt aatatgctga aatcagtact
     7741 gtgcgttacg taagcatcaa ataacggagt gatggcgccg ctgaaacagg cgacggcgaa
     7801 gataaaaaca atcagcttgg tgattgttgc ctggctaaat cttttccact ttgtatccaa
     7861 ttccccacac cacctttaaa tattttggtt ctttgggatt gatttcgatt ttctccctga
     7921 ttttgcggat gtgaaccgcc accgtgttct ccacgccaaa ggcattttcg ttccatactt
     7981 tttcataaat ctcgtcgatg gaaaagaccc ttcccgcatg ggcggtcaac aacttcagta
     8041 ttttatactg gacgggcgtc agtttgactt cctctccgtc taccgtcaca catttgctct
     8101 cgtcatcaat ttccagccct ccggtccgaa agacattgac ctttgtttcc aggctgccca
     8161 gcgtggtata tcttctcagc tgagatttta cccgggcgat cagttccaga ggattaaaag
     8221 gcttggtgat gtaatcatcc gctcccatgt tcagacccaa aatcttatcc gtatcctcac
     8281 tcctggcgga gagcatgatg accgggatgt tgttgttttc ccgaatcttt aaggtagccc
     8341 gcatgccgtc catttttggc atcatgatat ccatgatcac caaatggata gggttctctt
     8401 ccagta
//
LOCUS       DAOTHZ010000081         8307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_10332_length_8307_cov_7.424140, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000081 DAOTHZ010000000
VERSION     DAOTHZ010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10332_length_8307_cov_7.424140"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="era"
                     /locus_tag="P5549_08690"
     CDS             <1..688
                     /gene="era"
                     /locus_tag="P5549_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRW13188.1"
                     /translation="HLLGEYMVKVSTRSLDEVDLIYYMTDVTRPYGGGEQYIIEQLKS
                     TRVPIFLLVNKIDLVEEEKIKEFTQDFLNEISFSECLPISAYQKINLDLLLQKTFAYL
                     PEGPLYYPADDLTDQPMSFIVSELIREKALLLTRDEVPHSLAVSIEEFKHQGEQKIYV
                     RAVIHTERDSQKGIIIGKNGQMLKKIGEQARLEMEKMLSASVYLDLWVKVQKNWRDNE
                     GNLNQLGYRL"
     gene            734..1402
                     /gene="deoC"
                     /locus_tag="P5549_08695"
     CDS             734..1402
                     /gene="deoC"
                     /locus_tag="P5549_08695"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRW13189.1"
                     /translation="MNLASYLDNTNLKPEATHQEIAALCNEAARLKMAAVCIHACRLP
                     LAAEILAGSDVKLCTVIGFPLGADGRDSKVFAAAHALQSGAGELDVVINLGALKDGNY
                     QQVEEEVAQLLKLKKEYAFILKVIVETALLTSVELIALTRMLGDCGADFIKTSTGFSS
                     RGVSLQDLQIITAHRSSGLKIKAAGGIRELDFALQLIEAGADRLGSSSAGKILEEYKV
                     RGGC"
     gene            1417..2286
                     /locus_tag="P5549_08700"
     CDS             1417..2286
                     /locus_tag="P5549_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13190.1"
                     /translation="MLACHPDRIVHQAAYQTLAEMYGRIPLAGEDSQIYYNPGLRELP
                     QADNFLDIYPVLPGQEQSCLQQMEKVLASKAEVVVECSGLDSWHTSPTGAVWLPVLRE
                     RAYRVVIFDGGHHLPTLGMAPDLLIVPVLHGYAVHGYMRDGMRVDKLLEILSSNQIPA
                     VMATVPRWRLIKTADSLENTSRVILAQLRSTAAKADPFVPVCRPRVSKYNRHLFIYVN
                     NEYVKQPERLREYCRQLQLNDVQAAYIAFDYDVITPAQVRRYVQTLQDELGISLEIVN
                     QPVKVSSLLFKAD"
     gene            2310..3113
                     /gene="recO"
                     /locus_tag="P5549_08705"
     CDS             2310..3113
                     /gene="recO"
                     /locus_tag="P5549_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRW13191.1"
                     /translation="MSQRVWRKQFRRESELNYNSRGFVLRQRDYRDSDKLLNIFTEQK
                     GKITAIAKGIKKPKSSLRACAQPFCHAELHLHQGREIDLLTQARMLDFYAYSRSDLER
                     SLYCIYLMELLDKSLMENTPLPDLYQALLLVLTAINEEGLNLLFIRYFEARLLDNLGY
                     KPVLYHCISCQQQAEASFCLSLSEGGLVCPACQESGHEGIKVSGETMGLLRLLSEGSF
                     QILSRVKASPGGLRELEELLEKYLTYHLDRRFKMKNTIRLLKKTIHFEN"
     gene            3417..4331
                     /gene="glyQ"
                     /locus_tag="P5549_08710"
     CDS             3417..4331
                     /gene="glyQ"
                     /locus_tag="P5549_08710"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRW13192.1"
                     /translation="MNFQEIILSLQDYWSKQGCIIQQPYDLEKGAGTMNPATFLRCLG
                     PEPWKVAYVEPSRRPTDGRYGENPNRLQHYYQFQVILKPSPDNVIELYMESLRQLGID
                     PDQHDIRLVEDNWESPTLGAWGLGWEVWLDGMEITQFTYFQQCGGFDCHPVCAEITYG
                     LERIATYIQNKESVFAIEWVDGITYADVHHQSEVDYSHYNFHGADVEALITMFNICEQ
                     EARRVADLHLAQPAYDYVLKCSHLFNLLDARGAISVTERTGYIARVRNIARIVAKEYM
                     EQRQRLGYPLLKDDKLRQQLLQDVEVEG"
     gene            4331..6397
                     /gene="glyS"
                     /locus_tag="P5549_08715"
     CDS             4331..6397
                     /gene="glyS"
                     /locus_tag="P5549_08715"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRW13193.1"
                     /translation="MKRDLLLEIGVEEMPSAFMAGALNDIKELAEQKLTARRIGFDQV
                     DSLGTPRRLVLHVKGLEEKQEDAVIAARGPKKASAFDPQGNPSKAALGFARGQGIEAA
                     DLQIREVDGIEYVFAVKTELGGMTEEVLPEILLDIIHSLSFPKSMRWAYYQTRFARPV
                     RWLLTLYGNRTVNVQIENVFSGNSSYGHRFLAPDNFVVNSIDEYFAQLRQRYVIVDHR
                     ERKNLIWEQVQETARQAGGKAMENDELLEEVCFLLEYPTAFAGRFSPSYLDVPAEVLT
                     TSMIEHQRYFPVFDDQGRLMNAFIGVRNGTAASLDIVRAGNERVLKARLEDALFFWKE
                     DTAKALADFVPGLHKVMFHERLGSIMDKVERLQKLAVFIGQQTGLSCPDQLERAALLC
                     KADLLSNMVYEFPELQGIMGRYYALQSREEEEVAQAILEHYMPRFAGDELPASPTGTV
                     LALTEKLDNLTACFAIGIRPSGSQDPYALRRQALGVVNILTETNIKTDLRLIINAACQ
                     GLVNVKTEGDVQQFTDELMDFIRQRQRGILLDKGFAFDVIDAVLALSLSDLNDIQDRV
                     KAVQEFKATEKYEDFMVVFNRSHNLSKKWDSTAVNVEYLKDESEKALYQHLVQAQPLV
                     SQCLQAGDYTQAMHRLAALRVELDRFFTAVMVMVEDENLKAARLGMLKTIAGLCVQVA
                     DFSKII"
     gene            6509..7144
                     /locus_tag="P5549_08720"
     CDS             6509..7144
                     /locus_tag="P5549_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRW13194.1"
                     /translation="MELNERQIRILEIVKQNGPITGESIAELLEVTRAALRSDLAVLT
                     MSGYLEAKPRVGYTYKADGVDNKIRKVLNQYRVRDIMSMAVVVKENCSIYDAIVTLFV
                     EDTGSIFVLDTENYLAGVVSRKDLLKTTLGQADIHKMPVSVIMTRMPNIITTTGDETA
                     VEAVKKIVEHEVDSLPVVKCYLGPHNDEKCEVVGRITKTNIARIFLDLACK"
     gene            7162..7983
                     /locus_tag="P5549_08725"
     CDS             7162..7983
                     /locus_tag="P5549_08725"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRW13195.1"
                     /translation="MTEHLPGVFIVSDSIGETAEMVVRAAASQFNSGNMEIRRIPNIS
                     DQSILDEIVVQAAEKKYIIAYTLVLKDLADHLQLKASEAGVVCVDILGPIIEAFKNAS
                     HIEPKLEPGLLRKVDEMYYRRIEAVEFAVRYDDGKDPRGIALADIVLVGVSRTSKTPL
                     SMYLAHKRLKVANVPLVPEIAPPDELFSVERGKLIGLVIHPELLNSIRTERLKTLGLS
                     HQASYANEERILEELEYSDKIMKKLGCPVIDVTNRAVEETASKILEIYYRRLSNV"
     gene            7976..>8307
                     /locus_tag="P5549_08730"
     CDS             7976..>8307
                     /locus_tag="P5549_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRW13196.1"
                     /translation="MSSKKFIYLFEEGKSDMKNILGGKGANLAEMTKIGLPVPPGFTI
                     TAEACNVYQELKGDFPPGLMDDAFVALKKLEEKTGKHFGSKDNPLLVSVRSGAAVSMP
                     GMMDTILNL"
BASE COUNT         2172 a         1896 c         2249 g         1990 t
ORIGIN      
        1 gcatttgctg ggagagtaca tggtcaaggt atctacccgc agcctggacg aagtggattt
       61 aatctattat atgaccgatg ttacccgtcc ctatggcggc ggtgaacagt acattatcga
      121 gcagttaaaa tcaacccggg tgccgatatt tctgctggtc aataaaatag atctggtgga
      181 ggaagaaaaa atcaaagaat ttacccagga ttttctcaat gaaatcagtt tcagcgaatg
      241 tcttcctatc tctgcctatc aaaaaatcaa tcttgattta ctattgcaga agacctttgc
      301 ttatttgccg gaagggccgc tttattatcc agccgacgat ttgaccgatc aacccatgtc
      361 ctttatcgtg tcggaactga ttcgggaaaa ggcgctcctg ctgacccgtg atgaagtacc
      421 tcattccctg gcggtcagca tcgaagaatt taaacatcag ggtgagcaaa agatttatgt
      481 gcgagcggtg atccataccg aacgtgattc ccaaaaaggc attattatcg gcaaaaacgg
      541 gcagatgctc aagaaaattg gggaacaggc ccgcctggaa atggaaaaaa tgctgtctgc
      601 ttcggtttat ctggatctat gggttaaggt acagaaaaac tggcgagaca atgaaggcaa
      661 tttgaatcaa ttgggttatc gtttataatc tctggacggc attgccgcca acaagacaaa
      721 caggggggat tccatgaacc tcgcatcata cctggataat accaatctaa aaccggaagc
      781 cacccatcag gagattgctg cattatgtaa cgaagccgcc agattaaaaa tggcggctgt
      841 ttgcatccat gcctgccgac tgccgctggc agcggaaatt ttagcgggaa gcgatgtcaa
      901 gctgtgtacg gtaattggtt ttccactggg tgcggacggc cgggacagca aagtgtttgc
      961 tgcggcacat gcactgcagt ccggtgccgg ggaactggac gtggtgatca atcttggtgc
     1021 tctaaaagac ggcaattatc aacaggtgga agaagaagtt gcccagctgc tcaaactgaa
     1081 aaaagagtat gcttttatac ttaaggtcat tgtggaaaca gctttgctga cctcggtcga
     1141 gttaattgct ctgacccgca tgctggggga ttgcggagcg gattttatca agacttcgac
     1201 cggtttttcc agtcgggggg tttctttgca ggacctgcag atcattactg cccatcgttc
     1261 ttccggcttg aaaatcaaag cggcgggagg catcagggaa cttgactttg ctttgcaatt
     1321 aattgaagcc ggggcggatc gcttgggcag cagcagtgca ggcaaaattt tggaggagta
     1381 taaagtcagg ggaggctgct aaaggagcag acaacgttgc ttgcctgcca tccggacagg
     1441 atcgtccatc aggcagctta tcagaccctg gctgagatgt acggacgcat tcccttggcg
     1501 ggagaggaca gtcagattta ctacaacccc ggactgcgtg agctgcccca agcggataat
     1561 ttcctggaca tctatccggt tttgcctggt caggagcaaa gctgcctgca gcaaatggaa
     1621 aaagttttag cttccaaggc tgaagtagtg gttgaatgct caggccttga cagctggcac
     1681 accagcccga ccggcgcagt ctggctgcca gtgctgcgtg aacgggctta tcgggtggtc
     1741 atttttgacg gtggacatca tctgcctacc ctggggatgg ccccggatct cctcattgtg
     1801 ccggttctgc atggatatgc ggtgcatggg tacatgcggg acggaatgcg tgtcgacaaa
     1861 cttctggaga tcctttcatc caatcaaatc cctgcagtca tggccacggt tccccgctgg
     1921 cggctgataa agacagcaga ttctttggag aacaccagcc gggtcatcct ggcgcaactg
     1981 agatcaactg cggcaaaggc agatcctttt gtgcccgtct gtcgtccgcg tgtcagcaaa
     2041 tataaccggc acctgtttat ttatgttaat aatgagtatg tcaagcaacc ggaacgattg
     2101 cgggaatact gccgtcaatt gcaattgaac gatgttcagg cagcttatat tgcctttgat
     2161 tatgatgtta ttacaccggc ccaggtccgg cgctatgtac aaacattgca agacgaactg
     2221 gggatcagct tggaaattgt caaccaaccg gtcaaagtca gcagtctgct gtttaaggca
     2281 gactgatttt acacggatgg gggccgccga tgagtcaacg agtatggcgg aaacaattcc
     2341 ggagggagag cgaattgaac tacaacagcc ggggatttgt attaagacag cgggattacc
     2401 gggacagtga caaactgctg aacatattta ccgaacaaaa aggcaagatt acagcgattg
     2461 ccaaggggat caagaaaccc aagagcagtt tgcgggcctg tgcccagcct ttttgtcatg
     2521 ccgagctgca tctgcaccag ggtcgggaaa ttgatttgtt gacccaggct cgaatgctgg
     2581 acttttatgc ctattcccgc tccgatctgg agcgatccct gtactgcatc tatcttatgg
     2641 aactgctgga caagagcctg atggagaaca caccgctgcc cgatttatat caggcactgc
     2701 tgctggttct taccgccatt aatgaagaag ggttgaacct gcttttcatt cgctattttg
     2761 aggcccgcct gctggacaat ctgggataca agccggtgtt gtatcattgt atcagctgtc
     2821 aacagcaagc agaggccagc ttttgcctaa gtctgtctga aggcggtttg gtctgtccgg
     2881 catgtcagga gagcggtcat gagggaatca aggtcagcgg agaaacaatg ggcttgttgc
     2941 gtctgctcag cgaaggttct tttcagattt tgtcccgggt caaagccagt ccgggaggtt
     3001 tgcgggaatt ggaggaactg ctggaaaagt acctcaccta tcacctggac cgtcgtttta
     3061 aaatgaaaaa caccattcgt ctgctgaaaa aaacgatcca ttttgaaaat tgacaagccc
     3121 cattttgctt gttaaactag tataaatttc ataaccaatg acgatgaaga agaggagtag
     3181 gaaaggatag ccgcttcaag agagccgggc aaggtggaaa ccggcagggg ctttttccca
     3241 aggcgctttg gagtttcagg aggaaagccg caaaggtgag taaagcctgg caaagaaggt
     3301 gggctgcgtg ccaatcaggg tggaaccgcg ggtatatccc gtccctgttg ggtacgggct
     3361 ttttttattt caatgaaatg tttccgccca agccaaaaaa caaggaggta aaagctgtga
     3421 atttccagga gattatttta agtttgcaag actattggag caaacaggga tgtattattc
     3481 aacagccata tgatttggaa aaaggagcag ggactatgaa cccggcgacc ttcctgcggt
     3541 gtctgggtcc ggagccgtgg aaggtggcgt atgtggaacc ttcccgtcga ccgaccgacg
     3601 gacgctacgg agaaaatccc aaccgtctgc agcattatta ccagttccag gttatcctca
     3661 aaccatctcc cgataatgtg attgaactct acatggaaag tcttcgccaa ctggggattg
     3721 atcctgacca gcatgatatc cgtctggttg aggataactg ggagtcgccc accctggggg
     3781 cctggggact gggttgggag gtctggctgg acggtatgga aatcacccag tttacctatt
     3841 ttcaacagtg cggcggattt gactgccatc ccgtctgtgc agaaatcacc tatggtttgg
     3901 agcgcattgc tacttatata caaaacaagg aaagcgtctt tgcgatcgaa tgggtggatg
     3961 gtatcaccta tgcagacgtt catcaccaat ccgaggtgga ctattcccat tataatttcc
     4021 atggtgcgga tgtggaagcg ttgatcacca tgttcaatat ttgcgaacaa gaggcacggc
     4081 gagtggctga tcttcatctg gcccagccgg cctatgatta tgtactcaaa tgttcccatc
     4141 tgttcaactt gctggacgcc cgcggtgcta tcagtgtgac ggaaaggacc ggatatattg
     4201 cccgggtgcg caacattgcg cgtatcgtgg ccaaagaata tatggagcag cgccagcgtc
     4261 tcggctatcc gcttctgaaa gacgataaac tgcggcagca attgctgcag gatgtggagg
     4321 tagaaggata atgaaaagag atctgttatt ggaaatcggc gtggaagaaa tgccttctgc
     4381 tttcatggcg ggagcactga atgatattaa ggagctggcg gaacaaaaat tgaccgcccg
     4441 ccggatcggt tttgatcaag ttgacagcct gggcactccc cggcgactgg tcctgcatgt
     4501 caaaggcctg gaagaaaagc aggaagatgc ggtgattgca gcccgtggtc ccaagaaagc
     4561 cagcgctttt gatccgcaag gcaaccccag caaggccgcg ttgggctttg cccggggaca
     4621 gggaattgag gcagcggatt tgcagatacg tgaagtagac ggaattgaat atgtttttgc
     4681 cgtcaaaacg gaattgggcg gcatgaccga agaagtgctg ccggagattc tgctcgacat
     4741 catccattca ctgagctttc ccaagtccat gcgctgggcg tattaccaga cccgttttgc
     4801 ccggcctgtt cgttggctgc tgacccttta cggcaaccgg accgtcaatg tccagatcga
     4861 aaatgtcttc agcggcaata gcagctacgg tcatcgtttc ctggcaccag ataattttgt
     4921 tgtgaacagc attgacgaat actttgccca actgcgccag cgctatgtaa ttgtggatca
     4981 ccgggaaaga aaaaacctga tttgggagca ggttcaggag acagcccggc aggcaggcgg
     5041 caaggccatg gaaaatgatg agctgctgga agaagtatgc ttcctgttgg aatatccgac
     5101 cgcttttgcc ggccgttttt cgccttctta tctggatgtt ccggccgagg tcctgactac
     5161 atccatgatc gaacaccagc gttatttccc ggtttttgat gaccaggggc ggttgatgaa
     5221 tgcttttatc ggggtgagaa acggcaccgc ggcaagtctg gatattgtac gggccggcaa
     5281 tgaaagagta ttaaaagccc gactggagga tgccctcttt ttctggaaag aagacaccgc
     5341 caaggcgctg gcagattttg tgcccggtct tcacaaggtc atgtttcatg aaagactggg
     5401 cagcatcatg gacaaggtgg agcgtttgca aaagctggcc gtttttattg gccaacagac
     5461 aggactcagt tgtccggatc agctggaacg ggccgcgctg ctgtgcaaag ccgatctgtt
     5521 aagcaatatg gtctatgagt tcccggaact gcagggtatc atgggccgtt actatgcgct
     5581 gcaaagccgg gaagaagaag aagtcgccca agccatcctg gagcactaca tgcctcgttt
     5641 cgccggtgac gaattgcctg ccagccccac cggaaccgtg ctggcgctga cagaaaaact
     5701 ggacaacctg accgcttgct ttgccatcgg catccggcct tccggatccc aggatcccta
     5761 tgcgctgcgc cggcaggcgt tgggtgtggt caacatcctg actgaaacga atatcaaaac
     5821 cgatttgcgt cttatcatca atgcggcctg ccaaggtttg gtcaatgtta aaaccgaagg
     5881 ggatgtacag caattcacgg atgagctgat ggactttatc cggcagcggc agcgtggcat
     5941 tttgctggac aaaggctttg cctttgacgt gatcgacgct gttctggctt tgtccctgtc
     6001 cgatctcaat gatattcagg atcgggtcaa ggcggtacag gaattcaagg caacagaaaa
     6061 atacgaagat tttatggtgg ttttcaaccg ctcccacaac ctgtccaaga agtgggacag
     6121 cacagcggtc aacgttgaat atctgaaaga tgaaagcgag aaggccttgt accaacacct
     6181 ggtgcaggct caaccgttag tcagtcaatg tttgcaggcc ggggattata cgcaggccat
     6241 gcaccgtttg gcagctttgc gtgttgagct ggaccgcttc tttactgctg tcatggtaat
     6301 ggtcgaagac gaaaatttaa aagcggcccg gctggggatg ttaaagacga ttgccggatt
     6361 atgtgtccag gtagcagact tttccaaaat tatttaatcg ataaaaatga aattaggtat
     6421 tggcaaacag gattttattc gtcacaatag tatatatgat ttatgatatt ttacccaaaa
     6481 agtatagcgt aattgggtgg tgaagaaaat cgagctaaat gaacgacaga ttagaatcct
     6541 ggaaatcgtc aaacaaaacg gtcccataac cggcgaaagc attgcggaat tgctggaagt
     6601 gacccgggcg gcactgcgct ccgatctggc agtattgacc atgtccggtt atttggaggc
     6661 caaaccacgg gtaggctaca cttataaagc agacggagta gacaataaaa taagaaaggt
     6721 attgaatcaa taccgggtca gggatatcat gtcaatggcc gtcgtcgtca aggaaaactg
     6781 cagtatttat gatgccatcg tgacgctttt tgtagaggac accggcagta ttttcgtgct
     6841 ggatacagaa aactatctgg ccggcgtggt gtcccgcaag gatttgctga aaacgaccct
     6901 gggacaggcc gatatccaca aaatgccggt cagtgtcatc atgacccgca tgcccaacat
     6961 tattacaaca accggcgatg agactgctgt ggaagcagtc aagaaaatcg tcgaacacga
     7021 agtagacagt ctgccggtgg tcaaatgcta tctcggtccg cacaacgatg aaaagtgcga
     7081 agtcgttggg cgaatcacca aaaccaatat tgccagaata tttttggatc tggcatgtaa
     7141 ataacgaggg ggttaatcat tttgacagaa catttgcccg gtgtgtttat tgtgtctgat
     7201 tcaatcgggg aaactgcgga aatggtagtc agagcagctg ccagtcagtt caactccgga
     7261 aatatggaaa tcagacgcat tcccaacata tctgaccaga gtattctgga tgaaatcgtc
     7321 gttcaggcgg cagaaaagaa atatatcatc gcatacacgc tggtacttaa agatctggcg
     7381 gatcatctcc agctcaaagc cagtgaggcc ggagtggtat gtgtagacat tctggggccc
     7441 atcatcgagg cctttaaaaa tgccagtcac atcgaaccca agctcgaacc gggtttgctg
     7501 cgcaaggtgg atgaaatgta ttaccggcgg attgaggcag tagagtttgc cgtgcgctat
     7561 gatgacggca aagatccccg cggcattgcg ctggctgata ttgtactggt gggcgtatcc
     7621 cgcacttcca agacccccct atccatgtat ctggcccaca aacgtctgaa agtagccaat
     7681 gttccgctgg tgcctgaaat cgcccccccg gatgaattgt tctctgtaga gcggggcaag
     7741 ttgattgggt tggtcattca tcccgagctg cttaacagca ttcgcactga acgcctcaag
     7801 acactgggcc tttcccacca ggccagctat gccaatgagg aaaggattct cgaggaactg
     7861 gagtattcag ataaaatcat gaaaaaactt ggctgtccgg tcattgatgt caccaatcga
     7921 gcggtggaag aaacagccag caaaatattg gaaatatact ataggaggtt aagtaatgtc
     7981 tagcaagaag ttcatctacc tgtttgaaga gggaaaatca gacatgaaaa acattctcgg
     8041 cggcaaagga gccaatctgg ctgagatgac caagatcggt ctgccggtgc cgcccggttt
     8101 tacgataacc gctgaagcct gcaatgttta tcaggagtta aaaggcgatt ttccgccggg
     8161 gttaatggac gatgcttttg ttgctttgaa aaagctggaa gaaaaaaccg gcaagcattt
     8221 cggcagcaaa gacaatcccc ttttagtatc ggttcgttcc ggagcggcag tttccatgcc
     8281 gggcatgatg gataccatcc tcaatct
//
LOCUS       DAOTHZ010000082         8274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_10394_length_8274_cov_6.787200, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000082 DAOTHZ010000000
VERSION     DAOTHZ010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10394_length_8274_cov_6.787200"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2594
                     /locus_tag="P5549_08735"
     CDS             <1..2594
                     /locus_tag="P5549_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW13197.1"
                     /translation="SSGVVQNPLNGADRVFTYDLVDGDYAYTVKRSGYHSTFGRFSVN
                     GAAQSLTLPALTEPAGEAAEWPAFRDFSDNMASTASAIAQGAWQAEEQWSVSLGAPGP
                     WGTLTTSNLILYDGYLYAATEHGLSKLDRTSGKVLVTSALSADASYVSQIAYGDGRIF
                     VTTAAGVEAFDALTMESLWSCSDFDTFGSSYMATTPLLYDNGTVYVGTYGCSSGNNLG
                     TYGGYAAIGADDGRVLWTAWGGEHSVFYGAGAVRQGDYLIYGGDDGVLRAIDLAKVET
                     NAYHSLTTAPLQLPVNGAVRASVARAGEYLYLTTNSGYLYKIALDEDFKVENAIQFAP
                     ASTSTPLLHNGKIYVGASDGIYVFNGDDFTQLYHFPTTNPVQSSALLNDSGDGKVYAY
                     FTVNSAQGEILVLTDEGTELSCTVLYQPSPGQYCLNSLVADPDGVLYYSNDTGRVLAL
                     GNNHKPQGDWSTTTINITPSAVYEGDNTLYPTIKVITSSDQEIPAVAGHYYLIAGNYR
                     YNISLNGYLTAWGTFTVSADDAAVGSKEITVVLTKSSDSPSEVNLTVDFSLQDVSAAF
                     RRTITVAAGSTVYDVFIKAMNATGVDYTIRSGSYIEIIDGLAEGDRGANSGWMFTVNG
                     NHPVVGIAGYHVKSGDEIVFHYSSDYTREEDGQDWQAGDDSAENVAAITNDEPVSVVF
                     SDVEENAWYYTAVNFVNKRGLFGGTEAQVFAPDWAMTRAMLVMVLARLDGTDLTAFHN
                     PAFTDTQDDAWYAAAVAWSAARGIVAGMGDGCFAPDQEITREQMCVLLIRYCRAEGIP
                     LPEAAEHREFADDHAISDWSRAEVAMARHLGLIEGKIDNCFDPQGYLTRAEAATIFMR
                     FVQKFIP"
     gene            complement(2691..4523)
                     /locus_tag="P5549_08740"
     CDS             complement(2691..4523)
                     /locus_tag="P5549_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW13198.1"
                     /translation="MAANNFLFDTRDLKFILKEWLDLDKLLSFEPYRDYYSKNDVDAF
                     VDVVYKMCRDQLAPVNEDLDKIGARFIDGRVVVPESVRTAYHTVINAGFGPQFADREV
                     EGHLPRCMSAPLSEMIIAASAALFTYWSATDGVIGVIQEFGTPEIKQRFIPKLYDGIW
                     GGTMNLTEPSAGSEVGNTSTKALPTDKPGIYKIKGTKIFITSADNDIAENFVHLVLAR
                     VEGAREGTAGLSLFIVPKIWVNEDGTLGDDNDVTTVGIEHKMGLKGSATCTMSYGEND
                     ACYGIIFGNPPGEDGKGLGMAQMFKMMNEERLVTGLMALATSAEAYYNSLAYAKERVQ
                     GTKFTDPKGPRTRIIEHEDVRRMLLRQKSVVEAMRALIAKTYYYMDLANDSPDPEERA
                     FADARFQLSNPLCKAYISDMAWPLIAEAIQIYGGYGFTEEYPCAQLARDCKIFSIWEG
                     TNFIQSMDLVGRKFTMHKGTLLTSLLQDIQQFITENKQTPGFEREFAMLTEALSAYQE
                     ILGLLNEYYAQGQMTMGPLFSTRILHATAMIYCATLIMDQGLLASRKLDELGAEHFDA
                     GFYQGKAASARFYVMNVLPDIFGIKKAFAGGDTTAIDIPEEALG"
     gene            complement(4762..6594)
                     /locus_tag="P5549_08745"
     CDS             complement(4762..6594)
                     /locus_tag="P5549_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13199.1"
                     /translation="MAANNFLFDTRDVKFVLKEWLDLDKLLGCEAYRDYYSKDDVDTF
                     VDLVYKICRDQVGPANEDCDKTGAQYVDGRVILPESVKAAYHTICDAGLGPQKGDREV
                     EGHLPLLLSGAFDEMFSAASAGVTTLWNATAGAIGVIQEFAGEKYKALFIPKMYTGEW
                     AGTMNLTEPAAGSDVGNVSAKAFPTDQPGIYKVKGTKIFITSADNNIVENFIHLVLAR
                     IEGAREGTGGLSLFMVPKRWVNDDGSLGEVNDVYTLGIEHKMGLKGSATCTMSYGEND
                     ACYGILLGDPPGEDGKGKGMAQMFKMMNEERLVTGMMAQGISAEAYYNSLAYARERVQ
                     GTKSTDPKGPRVRIVEHEDVRRMLMRQKSCVEAMRAMIYKTYYYLDLSHDSPDPQERA
                     FADGRFQLSNPLCKAYISDMLWVLVGEAIQVYGGYGYIEEYPCAQLARDGKILAIWEG
                     TNYIQSQDLVGRKFSMAKGQVIASLLKDINDFIADHKNTPGFDKEFAMLASVMADYQE
                     ILALLNHYLQQGQNTMAPLYSTRILHATAMMYCAILIMDQALLATRKLAEVGEDHFDA
                     NFYKGKIASARFYVMNELPAIFSIRQIFAAGDTTALDVPEEALG"
     gene            6825..7553
                     /locus_tag="P5549_08750"
     CDS             6825..7553
                     /locus_tag="P5549_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRW13200.1"
                     /translation="MKRKMQYAGFLKQSLVDYPGEIAAVLFTRGCNLRCPFCHNPDLL
                     IKPKNPAPPVYDIEEIAAFLAERKGFIDAVVISGGEPTLQPQLKDDIKRIKELGYLVK
                     LDSNGTNSTVLEDLIRAGLVDYIAMDIKAPLEYKRYQQACGRLSSGDFFQVRNSIHLL
                     NHLSDVQVEFRTTVVPILHTPQDMEDIARYIEGASRYTLQQFNPRVTLEPAYGEVVPF
                     SKEEMADMAKLCAPYVKEVRVVNI"
     gene            7626..>8274
                     /locus_tag="P5549_08755"
     CDS             7626..>8274
                     /locus_tag="P5549_08755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRW13201.1"
                     /translation="MDSNLQLALKKYYGYTTFKPGQEDIINSILAGGDILGIMPTGGG
                     KSICYQLPALLMPGLTLVISPLIALMKDQVDALNELGITAACINSGMEAGQIRSCLVE
                     AHQGNYKLLYVAPERLASESFTNLLRNLPISLIAIDEAHCVSQWGHDFRPSYLDISPW
                     IEQLPQRPVVAAFTATATLPVREDITKLLNLVNPDVYVTGFKRENLHLSAIKGVDK"
BASE COUNT         1989 a         2078 c         2144 g         2063 t
ORIGIN      
        1 attccagcgg tgttgtgcaa aaccccctga atggcgcgga cagggtcttc acctatgatc
       61 ttgttgacgg agattatgcc tatacggtca agcgcagcgg ctaccacagt actttcggac
      121 gttttagcgt gaacggtgca gcccaaagct taacactgcc tgccctgact gagccggcgg
      181 gcgaggctgc agaatggcct gcctttcgag atttcagtga caatatggcg agcaccgcca
      241 gtgccattgc gcagggtgcc tggcaagcgg aagagcaatg gtcagtctca ttgggtgctc
      301 ccggtccctg gggaacgctg accacttcca atctcatttt gtatgacgga tatctgtacg
      361 ctgccacaga acacggtcta agcaaactcg accggacgag cggtaaggta ttggtgacca
      421 gcgccctgtc ggcggatgcc agctatgtca gccagattgc ttatggagac ggcaggatat
      481 ttgtcaccac ggctgccggg gtggaggctt ttgacgcgtt gaccatggaa tctctctggt
      541 catgcagcga tttcgatacg tttggcagtt catatatggc aaccacgccg ctgctctatg
      601 acaatggcac ggtatatgtc ggcacttatg gctgcagcag cggcaacaat ctggggacct
      661 acggaggata tgcggccatt ggggcagatg atggcagggt cctttggaca gcgtggggcg
      721 gagagcatag cgtattttat ggagcgggtg ccgtgcggca gggggattac ctgatttatg
      781 gcggagacga cggtgttctg cgggccattg atcttgccaa agtggagacc aatgcttatc
      841 acagcctgac gacagcaccg ctgcaactgc cggtcaatgg cgcggtgcgc gcgagtgttg
      901 cccgtgctgg cgaatacctg tatttgacga ccaacagcgg ctatctttat aaaattgcgc
      961 tggacgagga cttcaaagtg gaaaacgcaa ttcaatttgc tcccgccagc accagtactc
     1021 cgctgctcca caacggcaaa atctatgtcg gcgccagtga cggcatttat gttttcaatg
     1081 gtgatgattt tacgcagctg tatcatttcc cgaccaccaa cccggtccag tcctcagcct
     1141 tgttgaacga tagcggcgac ggcaaggtct atgcctattt tacggttaac tcggcccagg
     1201 gtgaaatttt ggtactgacg gatgaaggga cggagctttc ctgtaccgta ctctatcagc
     1261 catcgccggg ccaatactgc ctcaacagtt tggtggccga cccggacggg gtgctgtatt
     1321 acagcaacga caccggtcgt gtgctggccc ttggcaacaa ccacaagccc caaggggact
     1381 ggagcacgac caccatcaac atcactccat cggctgttta tgagggcgac aataccctat
     1441 atccgaccat caaagtcatt accagcagcg atcaggaaat cccggcagtc gccggccatt
     1501 attatctgat tgccggcaat taccggtata acatcagcct gaacggctat ctcactgctt
     1561 ggggaacgtt taccgtctct gccgatgacg ctgctgtggg cagcaaagag attaccgttg
     1621 tactgaccaa atcgtctgac agtccgtccg aggtgaacct gacggtggat ttttccctcc
     1681 aggacgtttc tgcggctttc cgcagaacca ttaccgttgc cgccggctca actgtctacg
     1741 acgtttttat caaagcaatg aacgcaaccg gggtggatta taccatcagg agcggcagtt
     1801 atatcgagat cattgatggt cttgcggaag gagaccgcgg ggccaattcc ggctggatgt
     1861 ttacagtcaa cggcaaccat cctgtggtgg gaattgctgg ttatcatgtc aagtccggag
     1921 acgagatcgt tttccattat tcttccgact atacccggga agaggacgga caggattggc
     1981 aggccggtga tgacagtgcc gaaaacgtgg cggccattac caacgatgag ccggtttcgg
     2041 tggtcttcag cgatgttgaa gaaaacgcct ggtattatac cgcggtcaat ttcgtgaaca
     2101 agcgcggctt gttcggcggc actgaagccc aggtttttgc tccggactgg gcaatgaccc
     2161 gcgccatgtt ggtgatggtg ctggccagat tggacgggac cgatctcacg gctttccaca
     2221 atcctgcgtt taccgatacg caagacgacg cctggtacgc ggctgcggtg gcctggtcag
     2281 cggcccgcgg cattgttgca gggatggggg acggctgttt cgctccggat caagagatca
     2341 cgcgggagca gatgtgtgtg ttgctgatca gatattgcag ggcggaggga atccccctgc
     2401 cggaggcggc cgaacatagg gagtttgctg atgaccatgc catcagcgac tggtcccggg
     2461 cggaagtcgc catggcacgc cacctcgggc tgattgaagg caaaatagat aattgttttg
     2521 atccccaggg atatcttacc cgagcggaag ccgcgaccat ttttatgcgt tttgtacaaa
     2581 aattcatacc ataatacccg cgggacattt gtattcagaa ataaatcatc agacttaaaa
     2641 cgggacgatt gactacccaa acggagtcaa ccgtcccgtt tgatctttgt ttagcccaac
     2701 gcttcctcag gaatatcaat ggcggttgtg tcgccgcctg cgaaggcctt tttaatgccg
     2761 aaaatgtccg gcagcacatt catcacgtag aaacgggcgc tggctgcttt gccttgatag
     2821 aaaccggcgt cgaagtgttc tgctcccagt tcgtccagct tgcgtgaggc caacaaaccc
     2881 tggtccatga tcagggtggc acagtagatc atggctgtgg catgcaggat gcgggtggag
     2941 aacaacggcc ccatggtcat ctgcccttgg gcgtagtatt cgttcaacaa tcccaaaatt
     3001 tcctgatagg cgctcaacgc ttccgtcaac atggcaaact cccgctcaaa tcccggtgtc
     3061 tgtttgtttt cagtaataaa ctgctgaata tcctgcagca ggctggtcaa caatgttcct
     3121 ttatgcatgg tgaatttgcg cccgaccaga tccatggatt ggataaagtt ggtcccctcc
     3181 cagatggaga agattttgca gtcgcgggcc aactgggcac agggatattc ctcggtaaag
     3241 ccgtaaccac cgtaaatctg aattgcttcg gcgatcagcg gccaggccat atcggatata
     3301 taagccttgc acagcgggtt gctgagctga aatctggcat cggcaaaggc gcgttcttcc
     3361 ggatcgggac tgtcgttggc cagatccata taataatagg ttttggcaat cagtgcccgc
     3421 attgcttcta caacagattt ttgccgcagc aacatgcgac ggacatcttc gtgttcaatg
     3481 attcttgtcc gcggtccttt gggatctgta aatttggttc cctgaacccg ttctttggca
     3541 taagccaggg aattgtaata agcttcagcg ctggttgcca gagccatcag gccggtaacc
     3601 agtctttcct cattcatcat tttaaacatc tgggccatgc ccaggccttt gccgtcttca
     3661 ccggggggat tgccgaagat aatcccgtaa caggcatcat tttcaccgta ggacatggta
     3721 caggtggcgg aacctttcag acccatttta tgttcgatac cgacggtggt gacgtcatta
     3781 tcatcaccga gtgtgccatc ctcattgacc cagattttgg ggacgataaa aagggacagg
     3841 cccgccgtgc cctcgcgggc tccttccacc cgggccagca ccagatgaac gaagttttct
     3901 gcaatgtcat tgtcagcaga agtgataaat attttggtgc ccttgatctt gtaaatacct
     3961 ggtttgtcag tgggaagagc tttggtggag gtattgccca cttcggaacc ggcggatggt
     4021 tcagtcaggt tcatggttcc gccccatatt ccatcatata atttggggat aaagcgttgc
     4081 ttgatttccg gggtgccgaa ttcctggatc acgccaataa caccgtcggt ggcactccaa
     4141 taggtaaaca gcgctgcact ggcagcaata atcatttcac tcagcggggc actcatgcaa
     4201 cggggcagat ggccttccac ctcccggtca gcaaactgcg gtccaaatcc ggcattgatt
     4261 accgtatgat aggcagtcct gaccgactcc ggaacgacga ctctgccatc gatgaaccgg
     4321 gcgccgattt tatccaggtc ttcgttaacg ggcgcgagct ggtcgcggca cattttatag
     4381 accacgtcca cgaaagcatc aacgtcattc ttgctgtagt aatcacggta cggttcgaag
     4441 ctcagcagtt tgtccagatc cagccattcc ttgaggataa atttgaggtc ccgggtgtca
     4501 aatagaaagt tgtttgctgc caatgataac gccctcctta aaatccaaat gcaatcaacc
     4561 aggttgattt atttcatata aggattattg tttttagtat tttaccatag aatgataaaa
     4621 taatttgaac tttcagcaga gacatgttga caatcgagat gaatgattta ggaagaaaaa
     4681 cggccctggt tcaacgaagt ttttgaacca ggaccgcaaa tgttttactg gctttgtctg
     4741 cttgacggga gacagttggt tttaccccag ggcttcttca gggacatcca atgcagtggt
     4801 atcgccggcg gcaaagattt gcctgatgct gaaaatggcg ggcagctcgt tcatcacata
     4861 aaatctggcg ctggcgattt tgcccttgta gaaattggca tcaaaatgat cttcgcccac
     4921 ttccgccagt ttgcgggtgg ccagcaaagc ctgatccatg atcaggatcg cgcagtacat
     4981 catggccgtg gcgtgcagga tacgggtgga atataagggc gccatggtgt tttgaccctg
     5041 ctggagataa tgattgagca gtgccagaat ctcctggtaa tcggccatga cggatgccaa
     5101 catggcaaat tccttgtcaa acccgggtgt atttttatga tcggcgataa aatcgttgat
     5161 gtctttcaga agactggcga taacttgtcc cttggccatg gaaaacttgc gtcccaccag
     5221 atcctgggat tgaatgtagt tggtaccttc ccagatggcc aagatcttgc cgtcacgggc
     5281 cagttgggca caagggtatt cctctatgta tccgtagcct ccatagacct ggatcgcttc
     5341 gcctaccaaa acccagagca tatcggatat gtacgctttg cacagcgggt tgctgagttg
     5401 gaatcttcca tctgcgaagg ctctttcttg cggatcggga ctgtcatgag acagatcgag
     5461 ataatagtag gttttgtaga tcatggcccg cattgcctcg acgcaggact tttgccgcat
     5521 caacatacgg cggacatctt catgttcaac gattctgacc cgtggacctt tgggatcggt
     5581 ggatttggtt ccctggactc tttccctggc gtacgccagc gaattataat aggcttcggc
     5641 ggagatgccc tgcgccatca taccggtgac cagccgttct tcgttcatca tcttaaacat
     5701 ctgggccatg cccttgcctt tgccgtcttc accgggggga tctcccaata agatgccgta
     5761 acaggcatca ttttcgccgt aggacatggt acaggtggcg gaacctttca gccccatttt
     5821 atgctctatc cccagcgtgt aaacatcgtt gacttctccc agagaaccgt catcgttgac
     5881 ccaccgcttc ggtaccataa aaagggaaag accgcctgtg ccttcccgag ccccttcaat
     5941 acgtgccagg accagatgga tgaaattctc cacgatgtta ttgtcagcgg aagtgataaa
     6001 tattttggta ccttttactt tataaatacc gggttgatcg gttggaaaag ccttggcgga
     6061 cacattgccc acatcagatc ctgcggcagg ttcagtcagg ttcatagtgc cggcccattc
     6121 gcccgtgtac attttaggaa taaagagtgc tttgtatttc tcgccggcaa attcctggat
     6181 taccccgatc gctccggcag tcgcattcca cagggttgtt acaccggcgg aggctgcgct
     6241 gaacatttcg tcaaaggcac cactcaaaag caagggcaga tgtccttcca cttcccggtc
     6301 gcctttttgc ggtcccaacc cggcatcaca gatggtatga taagccgctt ttaccgattc
     6361 cggcaagatg accctgccat cgacatactg tgcccctgtt ttgtcacagt cttcattggc
     6421 aggtccgacc tggtcacggc agattttata gaccagatct acaaatgtgt ccacatcatc
     6481 cttgctgtaa taatcccggt acgcttcaca tcccagcagc ttgtcgaggt ccagccattc
     6541 tttgagtaca aacttaacat cccgggtgtc aaacagaaaa ttatttgctg ccaatttgat
     6601 gcccccttta aaaaatctaa atataaatca acgagttgat ttaatttcaa aataaatgtt
     6661 atcttgtgaa gtattttacc ataaaatgca caataattga aatattgaaa atattaaaaa
     6721 taattaactg aaaatgtatt aaagataaat acccaaggga agccgtaatg atgcttatat
     6781 ggtataattt tgacatgacg aaaccataaa taccaagtaa ggctgtgaaa agaaagatgc
     6841 aatatgccgg ttttttaaaa caaagtctag tcgactatcc aggcgagata gccgcggtgc
     6901 tttttacgcg cggctgcaat ttgcgctgtc ccttttgtca taaccccgat ctgctgatca
     6961 aacctaaaaa tccagctccg ccagtatatg atatagagga aatcgcggct tttctggcag
     7021 agcgcaaagg attcattgat gcagtcgtca tcagcggggg cgagccaacc ctgcagccgc
     7081 agctcaaaga tgatatcaag cgtatcaagg agctcggcta tttggtaaag ctggacagca
     7141 acggaaccaa ttcaaccgtg ctggaggatt taattagggc agggctggta gactatatag
     7201 ccatggatat caaagcgccg ttggagtaca agcgttatca gcaggcttgc ggacggcttt
     7261 ccagcggcga ttttttccag gtgcgcaatt ccatccatct gctcaaccat ctcagtgatg
     7321 tgcaggtgga attccgtact actgtagtac cgatcctgca tactccccag gacatggaag
     7381 atatcgcccg ttatattgaa ggggccagtc gctataccct gcagcagttt aatccgcggg
     7441 tgacccttga gccggcttat ggcgaagtgg tacctttcag caaggaagag atggcggata
     7501 tggctaaatt gtgtgccccc tatgtaaaag aggtaagggt ggtcaatata taagcatgac
     7561 acctgctgcc aggaattata tcctgtcggc agcgaagcat tctaaataca agtacaggga
     7621 gaaaaatgga ttccaattta caactggcct taaaaaaata ttacggttac accactttta
     7681 aacccggtca ggaggatatt attaatagta ttctggccgg aggtgatatt ttgggaatta
     7741 tgcccaccgg gggcggcaag tcgatctgct accagctgcc tgcgctgctg atgcccgggc
     7801 tgaccttggt tatctctccg ctgatcgcgt tgatgaaaga tcaggtggac gccctgaatg
     7861 agctcggaat taccgccgcc tgcataaaca gcggcatgga agcgggacaa atccgcagct
     7921 gtttggtcga ggctcatcag ggaaactaca aactgcttta tgttgcgccg gaacgtctgg
     7981 cctcggaaag cttcaccaat ctgctcagaa atctgccgat ttccctgatt gccattgacg
     8041 aagcgcattg cgtatctcaa tggggtcacg actttagacc gtcctatctg gatatttcgc
     8101 cctggatcga acagctgccg cagcgcccgg ttgttgctgc ctttacggcc acagctaccc
     8161 tgccggtgcg ggaggatatc accaaattac ttaatctggt gaatccggat gtctatgtaa
     8221 ccggttttaa acgagaaaat ttgcatttgt ccgcgatcaa aggggtagac aaaa
//
LOCUS       DAOTHZ010000083         8134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_10635_length_8134_cov_7.747865, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000083 DAOTHZ010000000
VERSION     DAOTHZ010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10635_length_8134_cov_7.747865"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..488)
                     /gene="cas2"
                     /locus_tag="P5549_08760"
     CDS             complement(198..488)
                     /gene="cas2"
                     /locus_tag="P5549_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRW13202.1"
                     /translation="MMVLITYDVSTTNPAGQKRLRMVAKKCVDYGQRVQNSVFECLLE
                     PAQFVKLRNELEDIIDKNRDSLRFYYLGANWKRRVEHVGAKAGYDPEGTLVI"
     gene            complement(499..1527)
                     /gene="cas1c"
                     /locus_tag="P5549_08765"
     CDS             complement(499..1527)
                     /gene="cas1c"
                     /locus_tag="P5549_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HRW13203.1"
                     /translation="MRRLLNTLYITNPESYLAKEGENILVRIDDQTVFRVPCHNLEGI
                     VYFGYPGASPALLGLCAERGITVSFLTEHGRFLARIEGPQSGNVLLRRRQYRLADDEN
                     LSPSVSRYFIAAKILNSRNVLLRGRRDHPEQTGEKMNANIAMMGRMAKAVFECKKLDD
                     VRGIEGDIAQRYFEQLDHLILEDKEEFFMNGRNRRPPLDKFNALLSFFYTLLVHECRA
                     ALESVGLDPAVGFLHRDRPGRPSLALDLMEEFRAYLGDRMALSVINRKQVCRDDFVVN
                     ANRAVYLKPDPRKSLLTVWQKRKQEEIVHPFLGEKVPIGLLPYTQALLLARYLRGDLD
                     AYPAFVWK"
     gene            complement(1537..2217)
                     /gene="cas4"
                     /locus_tag="P5549_08770"
     CDS             complement(1537..2217)
                     /gene="cas4"
                     /locus_tag="P5549_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HRW13204.1"
                     /translation="MGLYSEEELLPISGLQHLAFCERQWALIHVEQQWRENQRTAEGR
                     IVHERVHDPSYKPHKPGWKVVRSMQLQSLRLGLFGEADVVEFFIRKTSQEPGDVGELS
                     SPRPVEYKRGKSKKGDYDRIQLCAQAMCLEEMMNCHIDEGDLYYAETRRREVVRLDDE
                     LRSVVTKLSEKMHRLYREGITPRPDKKLSVCRTCSMVNICLPKIRSQASGESYWREVL
                     ETVAPMDD"
     gene            complement(2222..3133)
                     /gene="cas7c"
                     /locus_tag="P5549_08775"
     CDS             complement(2222..3133)
                     /gene="cas7c"
                     /locus_tag="P5549_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HRW13205.1"
                     /translation="MIADFDKRYDFVIFFDVKDGNPNGDPDAGNLPRIDAETGHGLIT
                     DVCIKRKVRNYVTLKENDKPPYSIFIKEKSVLNNTIQQAYDSLQINLSEPPTDATDGK
                     KRNKTGQGQGKEIDRARRFLCQNFYDIRAFGAVLSTGANAGQVRGPVQLTFARSVDPI
                     VTLEHSITRMAVATEAEAEKQGGDNRTMGRKFTVPYGLYRAHGFVSAPLAAQTGFSST
                     DLELFWEALTNMFEHDRSAARGLMGTRKLFIFEHSSKMGNAPVQQLFDAITVKQVDTG
                     KPARDFRDYDVSINMEQIPQGVNVIVR"
     gene            complement(3164..4915)
                     /gene="cas8c"
                     /locus_tag="P5549_08780"
     CDS             complement(3164..4915)
                     /gene="cas8c"
                     /locus_tag="P5549_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HRW13206.1"
                     /translation="MILQALKEYYDRKAANQEIARDGWIKGNIDFLISLDPEGNILNI
                     SDLREQKGKKLVPRMLELPNIGKQALKHTNSGNDGNLLWDNASFVFGLGPKGDIRLQS
                     MIDAIDEWLGPCDDVGIAAVRKFLTNGLADRSHFDKVLNNSEYGEILNQGNARITFQV
                     PLTGFNLVIDSPAVSQALESAGQNIFPGCVIGTCLVTGQEGVVIEATHPVTKGVWGAQ
                     TAGASIVSFNKDAFNSYKKRQSYNAPVSKTAASQYTKALNTLLESSSQCMHIGDASTV
                     FWSEKRSSFEADFSLFFNEPPKDNPDVGTEKVKSLFESVSSGSYLDDDGDDRFYVLGL
                     APNAARIAVRYWQIGTIAEFACRIRQYFEDFSIVKPPNEPEFYSIWRILVNISTQDKS
                     ENIPPNLAGDFMTAILQGTPFPSTLLQACLRRIRSDVQNRVKPVRAALLKAYLNRYQR
                     FYPNPNQREVRKQLDINQPSIGYQLGRLFAVLEKIQEEANPGINATITERFYGAACAT
                     PVTVFANLIRLKNHHLAKIENKGRVINFEKLLGEIMGRLNDFPAHLDIQEQGRFAIGY
                     YHQRQAFFQPKAEGKDN"
     gene            complement(4912..5649)
                     /gene="cas5c"
                     /locus_tag="P5549_08785"
     CDS             complement(4912..5649)
                     /gene="cas5c"
                     /locus_tag="P5549_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HRW13207.1"
                     /translation="MCRWCLEVWGDYACFTRPEMKVERVSYDVMTPSAARAIFEAILW
                     KPAIRWNVTRIDVLNPIKWISVRRNEVGRIVSEPTAKQMSGVVGAPMGIFIEDERQQR
                     AGLFLRDVRYRIYAYFDFLSPEERKTNYTAAPELWADAGEQSEVQRLDEKEAKYAAMF
                     ERRARKGQCFHRPYLGCREFACDFKLIKNPQEEPAETINVTMDLGYMLYDLDFTQDAS
                     NPNPLFFRAQMNNGVINTDRREVDVRG"
     gene            complement(5666..7993)
                     /locus_tag="P5549_08790"
     CDS             complement(5666..7993)
                     /locus_tag="P5549_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HRW13208.1"
                     /translation="MKACALLCEVTRKCALPIRKGGGNIRNVSEELIAHVTRNADGSW
                     RTPQTLEEHLEKTAQKAELFASKFDSGVWGKTISWAHDVGKGRPAWQNSIRFRTGFNA
                     FDEEAGLEGMPGKIPHAIQGAMLAEQIFGKQRGRIMAYCIAGHHAGLPDWSSAAGTGK
                     ASLEYQESQAPGLDDIDPSIVDGLSSLEPGMPPWKFANGLDMPLWIRMLFSCLVDADF
                     LDTESYMDHDKASLRGEYAPLGLLLERFNEYNLKLDRDSDNTTVNEIRRKVRSKCIQM
                     AAEDQGIFSLTVPTGGGKTLSSLAFGLNHAIKHSLDRIIYVIPYTSIIEQNAEVFRAV
                     VGSEQVVEHHSNLDEKDSTYPSRLAAENWDAPIIVTTAVQFFESLFASRPGRCRKLHN
                     IARSVVVLDEAQLLPAKFLQPILDTMQLLSDRYRVSFVISTATQPAFGQRMIGDSKFR
                     GLKNIREIMGSPEEVDQIYRGLERYQVYFPPDLQVKSEWEDIARELKQYEQVLCVVSD
                     RKSCRELYSLMPTGTYHLSALMCGQHRSEVITQIKQKIRDGSPVRVISTQLVEAGVDL
                     DFPIVYRAFAGLDSIAQAAGRCNREGKLPQPGRVVVFIPPRPAPMGILRKAAETTVNI
                     VNGNREDPVHHSLFGRFFSELYWKINSLDENNIIKLLDPKQNDIKECSMYFRTAAARF
                     RLIDESTHAIVVPYHDGAALIDSLKTNGPDWRLLRKLQRYTVNIYDQEFDQMMKRDAL
                     FEASPGVFALMSDADYHPDIGLMVEPYFDPDRFIL"
BASE COUNT         2018 a         2128 c         1762 g         2226 t
ORIGIN      
        1 tttcaatcca cgcacccgcg aggggtgcga cagtatgact taaaaacaaa ggtatttcgg
       61 tgcattatat ctgctgttcc gcgaatagct gcttgtggcc gttacggtta cacctgtgct
      121 gggctattat ttgctgaaga caccgctact aagccatcgc gaacattccg tgattttcca
      181 atcacttgcg gttcgcgcta aatcactagt gtcccttccg gatcataacc ggctttggca
      241 cctacatgtt caactctccg cttccaattg gcccctaaat aataaaacct taaactgtct
      301 ctgttcttat cgatgatatc ttccagttca tttcgcaatt tcacgaattg agccggttca
      361 agcaggcatt cgaagaccga attctgtacc cgctggccat aatctacaca ctttttggcg
      421 accattctca acctcttctg gccggcagga ttagtagtgc ttacatcgta ggtaatcaga
      481 accatcatgg ctatcacctc atttccaaac aaaggccgga taggcgtcca aatcaccgcg
      541 caagtatctg gccaacaaca aggcctgagt gtacggcagt aatcctatcg gcaccttttc
      601 acccaaaaaa ggatgcacaa tctcttcctg tttgcgtttt tgccatacag ttaagaggct
      661 cttcctggga tcgggcttta ggtagaccgc ccggttggca ttgacaacga agtcatcccg
      721 acacacctgc ttgcggttga ttacggaaag agccatccta tcaccgagat aagcccggaa
      781 ctcctccatc agatcaagtg ccaggctggg acgcccaggg cgatctcggt gcaggaatcc
      841 gacagcgggg tcaagtccga cgctttccag agcagcacgg cattcatgaa ccagcagagt
      901 atagaaaaaa gacagcaagg cattaaactt gtccaaaggc ggtcttcgat tgcgcccgtt
      961 cataaaaaac tcctctttgt cctccaggat cagatggtcc agctgctcaa aataacgttg
     1021 ggcaatatca ccctctatac cgcgcacgtc atccagtttc ttacattcaa aaaccgcctt
     1081 ggccatgcgc cccatcatgg caatattggc gttcatcttc tccccagttt gctccgggtg
     1141 gtcgcggcgt ccacgcagca ggacgtttcg gctgtttagg attttagcgg caataaaata
     1201 cctggacact gacggtgaca agttttcgtc atcagcgaga cgatattgcc tacgccgcaa
     1261 caggacattg ccactctgag gtccttctat ccgggccaaa aaccttccgt gctcggtcag
     1321 gaaactcacg gttatccctc tttctgcaca taaacccagc aaggctggac ttgcgcccgg
     1381 gtagccaaag taaacgattc cctcaagatt atgacatggt acccgaaata ccgtctgatc
     1441 atcaattcga accagaatgt tttcgccttc tttagccaga taggattccg gatttgtgat
     1501 atacaacgtg ttcagcaatc ttctcaataa atcacctcaa tcatccatcg gcgcgacagt
     1561 ctccaatacc tcacgccaat aggattctcc gctggcctgc gagcgtattt tgggaagaca
     1621 aatattaacc atggaacacg tccggcagac actgagtttc ttatcaggtc ggggggttat
     1681 tccttctcgg tacaaacgat gcattttctc ggacaatttg gttaccacac ttctgagttc
     1741 atcatctaat cttactacct cgcgccgcct ggtctcagcg tagtacaaat caccctcgtc
     1801 gatatggcag ttcatcattt cttccaggca catggcttga gcacataatt ggatgcggtc
     1861 ataatcaccc tttttgcttt ttccccgctt gtattcaacc ggcctgggcg atgacaattc
     1921 ccccacgtcc cctggttcct gtgaagtctt ccttataaag aattcgacta cgtctgcttc
     1981 accgaaaagc ccgaggcgca gagactgcag ctgcatgctg cgaacaacct tccaaccagg
     2041 tttatgtggt ttataggagg gatcatgcac cctttcatgt acgatacgcc cttctgctgt
     2101 ccgttggttc tcccgccact gctgctcaac atggatcaac gcccactgtc gctcacaaaa
     2161 agctaggtgc tgcagaccgg aaatgggcag aagctcttct tcggaatata gccccatctg
     2221 tttatctcac tatcacgttc acaccctgag gtatctgttc catatttatc gaaacatcat
     2281 aatccctaaa atcacgagcc ggcttgccag tgtcaacctg cttaacggta attgcatcaa
     2341 agagttgttg aactggggca ttgcccatct tcgacgagtg ttcgaatatg aataacttcc
     2401 tggttcccat taaaccacga gcggcagaac ggtcatgctc gaacatgttg gtcagtgctt
     2461 cccagaacag ttccagatcc gtagatgaaa accctgtctg ggcagctagg ggagcggaaa
     2521 caaatccatg agcacgatat aatccgtagg gaaccgtaaa tttccttccc attgtgcggt
     2581 tgtcgccacc ttgtttctca gcctcggctt cagttgccac cgccattctg gttatgctgt
     2641 gctccagagt tacaatggga tctacggagc gggcaaatgt caactggact ggaccgcgta
     2701 cttggcccgc attggcaccg gtcgagagga ccgctccaaa cgccctgata tcatagaaat
     2761 tttggcaaag gaaccgccgg gcacgatcaa tttccttacc ctgaccctgt ccagtcttgt
     2821 tgcgtttttt gccatcagta gcatcagtcg gcggttcact gaggttaatt tgcagactgt
     2881 cataggcttg ctggatggtg ttgttgagaa cggatttttc cttaatgaag atactatagg
     2941 ggggcttatc attttcctta agggtcacat agtttctgac ttttcgttta atgcagacat
     3001 cggttatcaa tccatgacca gtctcggcat cgatacgtgg caggttgcct gcatcagggt
     3061 cgccgttggg attgccatcc tttacatcga aaaaaatcac aaagtcataa cgtttatcaa
     3121 aatctgctat cattatagac ttcaccctct ctaaattaat tgattaattg tccttgcctt
     3181 cagccttggg ctggaaaaac gcctgtcgct gatgatagta acctatggca aaacggccct
     3241 gttcttgtat atccagatgg gcggggaaat cattgagtct tcccattatt tcaccgagca
     3301 gcttttcaaa atttattacc cgccccttgt tttctatctt agctaggtga tggttcttga
     3361 gccggatcag attggcaaaa accgtaaccg gagttgcgca ggccgcacca taaaaacgtt
     3421 cggttatggt tgcgttgatg cctgggttag cctcttcctg aatcttttcc aggacagcga
     3481 ataaccgtcc taattggtac ccaattgatg gttgattgat atccaattgc ttccttacct
     3541 ccctctgatt tggattagga tagaatcttt gataacgatt caaatatgcc ttcagcaacg
     3601 ctgcccgcac cggcttcaca cggttttgga catcgctgcg aattcttctt agacacgctt
     3661 gaagtagtgt agatggaaaa ggagttccct gcagaatcgc ggtcatgaaa tccccggcaa
     3721 gatttggcgg gatattttca cttttatctt gagtagagat attaaccaaa atccgccaga
     3781 ttgaatagaa ctctggctcg ttcggtggtt tgactatgga gaagtcttcg aagtactgtc
     3841 tgatgcggca ggcaaactct gcgatagtcc caatttgcca ataacggaca gcgattctgg
     3901 cagcatttgg agctaggcct aatacataaa accgatcatc tccgtcatca tcaagatagc
     3961 tgccgctgct aactgattcg aatagagatt tcactttttc tgttccaaca tcgggattat
     4021 ctttcggtgg ttcattgaaa aacaatgaaa aatctgcttc gaatgaagat ctcttttctg
     4081 accagaagac ggtggatgcg tcgccgatgt gcatacattg gctggacgat tccagcaaag
     4141 tatttaatgc cttggtatat tgtgacgctg ctgttttact tactggagca ttatagctct
     4201 gtcgtttctt atatgaattg aacgcgtctt tgttaaaact gacaatactc gcccctgcag
     4261 tctgtgctcc ccatacgcct ttggtaacag gatgggtggc ttcaattaca acaccctctt
     4321 gccccgttac cagacatgtc ccgattacac agcccggaaa aatgttttgg cccgcagact
     4381 cgagagcttg tgatactgca ggagagtcga tgaccaggtt gaaaccggtt agaggcacct
     4441 ggaaagtaat tcgggcattg ccttgattaa gaatttcacc atattcggag ttattcaaca
     4501 ccttgtcaaa atgtgaacgg tccgctagac cattggtaag aaactttcga acagctgcaa
     4561 ttccaacatc gtcacatgga ccgagccact catcgatggc atcgatcatg ctttgcaggc
     4621 gaatatctcc tttgggtcca agtccgaaca cgaatgatgc attatcccag agcaaattac
     4681 catcattccc gctgttcgtg tgttttaatg cctgtttgcc gatgttgggc aattccaaca
     4741 tgcggggaac gagctttttc cccttttgtt ctcgtaaatc cgatatgttc aatatgttcc
     4801 cttccggatc gagacttata agaaaatcaa tgttcccctt tatccacccg tcccgggcaa
     4861 tttcttgatt ggctgctttg cggtcgtaat actcttttaa cgcctgtaat atcatcccct
     4921 gacatccacc tcccttcggt cggtattgat aaccccatta ttcatttgcg ccctgaagaa
     4981 tagcgggtta ggattgctcg catcttgagt gaaatcgagg tcgtataaca tataacccag
     5041 atccatagtg acattgattg tttcggctgg ctcttcctgc gggtttttta tcaacttaaa
     5101 gtcgcaggcg aactcccggc atcccaagta aggccggtga aaacattgac ctttgcgagc
     5161 acgccgctcg aacatggcgg catacttggc ttccttttca tctaatcgct gcacctccga
     5221 ctgctctcca gcatcagccc acaattcggg agcagcagta taattggtct tacgttcttc
     5281 aggggaaaga aaatcaaaat atgcgtagat tcggtatctt acatctcgca ggaacaaccc
     5341 cgcccgttgt tgacgttcat cttcgataaa aatacccatc ggagcgccta cgactcctga
     5401 catctgctta gcagttggtt ccgatacaat tcggcctacc tcattacggc gaactgatat
     5461 ccacttaatg ggattcagta catctatccg ggttacattc cagcggatag ccggtttcca
     5521 taggatagcc tcgaatattg ctcgggctgc tgacggggtc attacgtcat agcttacccg
     5581 ctcgaccttc atctctggac gggtgaagca tgcgtaatcc ccccacactt ccaaacacca
     5641 cctgcacaaa tacaccaact cctttctata agataaatct atctggatca aaataaggct
     5701 ctaccatcag accaatgtcc gggtggtagt cagcatctga cattagcgcg aagacaccag
     5761 gagatgcttc aaacaaagcg tcccgtttca tcatttgatc gaactcttgg tcatagatat
     5821 tcacagtgta gcgctgcagt ttacgcagta agcgccaatc aggcccattg gttttcaacg
     5881 aatcaatcag agctgcacca tcatggtaag gcactactat agcgtgagtg gactcatcga
     5941 tgaggcggaa acgggcagca gcagtccgga aatacatgct gcattcttta atatcgttct
     6001 gcttggggtc aagcaattta atgatgttat tttcatctaa cgagttgatt ttccaataca
     6061 gctctgaaaa aaacctacca aacagactat ggtgcacggg atcttctcgg ttgccgttca
     6121 ctatattcac tgtggtttct gctgccttgc gcagaatccc catcggggct ggacgaggtg
     6181 gtataaaaac aaccactctt cctggctgag gcagcttgcc ttcccggtta cagcgccctg
     6241 ccgcttgagc gatcgaatca agaccggcaa aagctctgta aacaatcgga aaatcaaggt
     6301 cgacgcctgc ttccactaac tgcgtactaa taacccgaac cgggctcccg tctctgatct
     6361 tttgcttgat ttgggttatg acctcgctgc gatgctgccc acacattaag gcagacagat
     6421 gataggttcc agtcggcatc agactataca gttcccggca gctttttcgg tcggatacca
     6481 cgcataaaac ctgctcatat tgtttcaact cccgggcaat gtcctcccat tccgatttga
     6541 cctgcagatc gggcggaaaa tacacctgat acctctccaa gcctctatag atctgatcga
     6601 cttcttccgg actccccatg atttctctta tatttttcaa tccccgaaac ttactatcac
     6661 ctatcatgcg ctggccaaat gcaggttgcg tggctgtgga tatgacgaag ctgactcggt
     6721 agcgatccga cagcagttgc atagtatcta ggatgggctg caaaaatttg gccggcagca
     6781 gttgagcttc atccaacaca accactgaac gagctatatt gtggagcttg cggcagcgtc
     6841 cggggcggga ggcaaacagc gactcgaaga actgtactgc ggtggtaaca attatcgggg
     6901 catcccaatt ctcagccgct aatcgtgagg ggtaagtcga atccttctcg tccagattgg
     6961 agtgatgttc gaccacctgc tcggagccca caactgcccg aaatacttct gcattctgct
     7021 cgatgatact cgtgtaaggg atgacataaa tgatgcggtc aagactgtgc ttaatcgcat
     7081 ggttaagccc aaaggcaaga ctggaaaggg ttttccctcc tccggtaggt acggttagag
     7141 aaaagatgcc ttgatcctct gcagccattt gaatacactt ggatcgtact tttcgtctaa
     7201 tctcattaac ggtggtatta tctgagtctc gatcaagttt caggttatac tcgttgaagc
     7261 gttccagaag cagacccaat ggagcgtatt cgcctcgcaa agacgcttta tcatgatcca
     7321 tatacgattc ggtatccaga aaatcagcat ctaccaagca ggaaaagagc attcttatcc
     7381 ataaaggcat gtccagtcca tttgcaaatt tccatggtgg catcccgggt tccagcgaag
     7441 ataacccatc gacaattgag ggatcaatat catccagtcc cggagcctga gattcttgat
     7501 actccaatga agcctttcca gtaccagcag cgctggacca atccggcaat cccgcgtgat
     7561 ggcccgcgat acaatacgcc attattctcc ctctctgttt gccgaatatc tgttctgcca
     7621 acattgcccc ctggatagca tgaggaatct tacccggcat tccttccaga cctgcttcct
     7681 catcgaatgc gttaaatccc gttctgaacc tgatagaatt ttgccaggca ggtcttcctt
     7741 tgccgacatc atgagcccag ctaatggttt ttccccacac ccccgagtcg aatttacttg
     7801 caaaaagttc agctttctgc gcagtcttct ccaaatgttc ctccagggtt tgaggtgttc
     7861 gccaactccc gtcagcattt ctagtcacat gtgcaattag ttcttcgctt acatttcgga
     7921 tatttccacc tccttttctt atcggcaagg cacactttct tgttacttca cataacagtg
     7981 cgcatgcttt catataacac tccccaacta ttatttccat atcttttctt aatttcctgc
     8041 aatttagaca tttttattat tcatacgaaa tggatttttc caatgtaggc cacagcatag
     8101 ctgatggcct cttggagaaa cgagcgctaa tggc
//
LOCUS       DAOTHZ010000084         7866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11098_length_7866_cov_7.091538, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000084 DAOTHZ010000000
VERSION     DAOTHZ010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11098_length_7866_cov_7.091538"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="P5549_08795"
     CDS             <1..2085
                     /locus_tag="P5549_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRW13209.1"
                     /translation="ILSPAELIDVYHLLRASRLAIKYTATPSARILKNIGSGLTENKT
                     LEQMISDAVDEDGTIRDDASSALRSIRKQIETSRLRIKDYLQNFIRSGNNQNLLQDAL
                     VTERAGRYVVPVKQEYRNEVKGIVHDESASGATVFIKPLPVVEHNNKIKSMQIEEQRE
                     IERILRELSAAVSVSSDELQTNNEILEELDVIFARANLAYNMNSFRPRINDRGMIDLQ
                     KARHPLLGRQAVPININLGQTFDLLVITGPNTGGKTVVLKTVGLLTVMAMSGLFVPAR
                     EDSQLSVFKNIYVDIGDEQSIEQSLSTFSSHMKNMIRILQQAGKGSLVLLDELGAGTD
                     PHEGAALARAILQRLKNLQAKVVVTTHQSELKTFAYQNQRVENACVEFNPVTLLPTYE
                     LTIGTPGQSNAFAIASRLGLEQAIVEQARSLVPEREREIGDMIRQLKESRYSFEESSR
                     ELEKGQRDLERERRELSSERETLLTMKNEIMRKARQEADSYVRAIKREANEAIDELKG
                     ILKEKEKPPKWHEVEEKRKRLRNLSVSFEDDEEGLASDDFKAGDYVQLKSIRQFGYVL
                     EAPNNQGDVMVQIGATRLSVNREQLLPAQLQPEKPQRYQQTFVEKARHISPELDLRGK
                     YAEEALEELDKYLDDAALAGIEKVRIIHGKGTGALRRAIRSHLQNHHFVSEFRDGAIA
                     EGGFGVTVISLK"
     gene            complement(2160..4583)
                     /locus_tag="P5549_08800"
     CDS             complement(2160..4583)
                     /locus_tag="P5549_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRW13210.1"
                     /translation="MPDDFYLDYKYDYPQPPPRSKWKTIFSFLLFAGIGLFLGVIVYS
                     AYALPAFNEEQLTGANASLLYDDQGKIFWRVHAEEDRTSIELDKVPDSLIKAFIATED
                     KEFYNHHGVNYKGIARALLSNLQSRDLTGQGASTITQQLARDAFLSQEKTWQRKVKEI
                     LLAYKLESLYSKDEILELYLNKIYFGAGAYGVQAAANKYFAKDISELNLAESSLLAGL
                     VQSPSRYDPYQNLEAAKARQWLVLLSMRDSHFISYSEAEEAHQAELVLKSGQSNSMNY
                     GFFIDAVIEESIKHLSKVKGYKDADAMIYSSGLKIHTTMDAALQKHAEEYFKDPAHFP
                     SETKNNNPIQVGMAIFDHSNGEIKAIMGGREYTNQRGFNRATSAFRQPGSAIKPLTVY
                     APALEKGYMPYTVLDDSPISYKVNNEIWSPKNYDHSYRGLISMRTAVQYSVNTYAIQL
                     LDKVGISNGYETGKALGLNHLVNTPGRNDLGLSPLALGSLTKGASPVEMAAAYGSFGN
                     GGYYAQPHFITKITDHHGRVLYAYKQPMKRALSADTAWLMNSMLQTVANSGTGTNGKV
                     PGVPTGGKTGTSENNNDCWFCGLTPLYSGAVWMGYDETYTMQNQYGGGYPAKLFRSMM
                     QKAHEDKEYQPWAKPHNVVLTSICSKSGKLPSSSCPDEQIISEYCVGKYIPKDVCTLH
                     QKVTICRESGKLATKYCPDIYDQAMVQAKPGSAEETKIPTETCDIHTSFTIQSLFKNT
                     VKICTDPRHEGKLYQAVTPHGGQSGGCPPAMIKEVVVNPGQHLAPCPLKDHQIHKGPG
                     HDLIDNQAE"
     gene            complement(4873..5124)
                     /locus_tag="P5549_08805"
     CDS             complement(4873..5124)
                     /locus_tag="P5549_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13211.1"
                     /translation="MRTLVATALVNSKGKDIYCTAKKITDKHMDYIRNYDRKTLEEIG
                     FVFIKMMSLDYPNVKGHAIFFEGHINDIMPALKSLGKRY"
     gene            complement(5232..5307)
                     /locus_tag="P5549_08810"
     tRNA            complement(5232..5307)
                     /locus_tag="P5549_08810"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5271..5273),aa:Glu,seq:ctc)
     gene            complement(5315..5388)
                     /locus_tag="P5549_08815"
     tRNA            complement(5315..5388)
                     /locus_tag="P5549_08815"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5354..5356),aa:Gln,seq:ctg)
     gene            complement(5482..6936)
                     /gene="gltX"
                     /locus_tag="P5549_08820"
     CDS             complement(5482..6936)
                     /gene="gltX"
                     /locus_tag="P5549_08820"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRW13212.1"
                     /translation="MGEMRVRFAPSPTGTLHIGGARTALFNWLLARSQQGSLVLRLED
                     TDLERSTAESAHGIVEGLRWLGLDWDEGPDIGGPYGPYRQSERLSIYQHYLQQLLDSG
                     HAYYCFCTADELRRQRESARHDQDAFRYDGTCRSLSATEVAHRLSLGLKPTVRFKMPQ
                     QGITAVHDLVRGQVEFQNDLIDDFIIAKSDGWPTYNFAVVVDDFTMQISHVIRAEEHL
                     PNTPKQLLIYQALGLTPPQFAHVSMILAPDRSKLSKRHGATSVQEFRDQGYLPQALVN
                     YLALLGWSPGDDLDLMDREEMIRRFDLAHFSKSAAIYDIEKLAWMNGHYLAELSAEDI
                     LSRVKEEAWARGWLTTDNHAYFIKIITLLRSRIRIIQELWPAAEYFFAEPQLYDEKGV
                     DKYFRQSACRERLAAVKSLMDMQPFDAGQIEQAIRSRAEAMGLKAAELIHPTRLALTG
                     RTNTPGLFEIMELLGPEVCSRRLARALAFIEAFK"
     gene            7251..>7866
                     /gene="yunB"
                     /locus_tag="P5549_08825"
     CDS             7251..>7866
                     /gene="yunB"
                     /locus_tag="P5549_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HRW13213.1"
                     /translation="MYYRRRQKAAVLVLLLVLLASIVMIDFRIKKSLLELAQTQAEIR
                     TVEIVTRAVYEQLGQATEYNDIVHIHKDDQGRIVMLQANTVILNRYMALANKAILAGL
                     KQANADQIKIPLGQITGITLLAGRGPRLGVRVMPASQVFIEVDDRFEEAGINQTRHRI
                     YMKVKTRIKIAVPLMGKEILVVSTIPMTETVIVGDVPQTYVSLKG"
BASE COUNT         2040 a         1894 c         2002 g         1930 t
ORIGIN      
        1 attctgtcac ccgccgagct gattgacgtc tatcatttgc tgcgcgcctc gcgattggcc
       61 ataaaatata ccgcgacacc gtccgcccgg atcttgaaaa acatcggcag cggtttaacg
      121 gaaaataaaa ccctggaaca gatgatcagc gatgcagtgg acgaggatgg aacgattcgt
      181 gatgacgctt cctcagcttt gcgcagtatc cgcaagcaaa tcgaaaccag tcgtttgcgg
      241 atcaaggatt acttgcagaa ttttatccgt tccggcaaca accagaacct gttgcaggat
      301 gcgctggtaa cagaacgtgc tggacgatac gtggtaccgg tcaagcagga atatcgcaat
      361 gaagtaaaag gcatcgtcca tgatgaatca gccagcgggg cgacggtttt tatcaagccg
      421 ctgccggtgg ttgaacacaa caacaagatt aaaagcatgc agatcgagga acaaagagag
      481 atcgaacgta tcctccggga gctgagtgca gcagtttcag tgtcttccga tgaattgcag
      541 accaacaacg aaattctgga ggaactggac gttatttttg cccgggccaa tctggcctac
      601 aatatgaata gttttcggcc gcgtatcaac gatcgcggca tgatcgatct gcaaaaagcc
      661 cgtcatccat tgttgggcag gcaggcggtg ccgatcaata taaacctggg gcagaccttt
      721 gatttgctgg tcattaccgg acccaatacc ggtggcaaga cagtggtttt gaagacagta
      781 ggtcttttaa ccgtaatggc tatgagcggc ttgtttgtgc cggccaggga ggacagtcag
      841 ctctcggttt ttaaaaacat ttatgttgat atcggcgatg aacagagtat cgaacaatca
      901 ttgagcacgt tttcctcgca tatgaaaaat atgattcgca ttctgcagca ggccggcaaa
      961 ggatcgctgg tgctgttgga cgaactgggc gccggaactg acccgcacga aggcgcagcc
     1021 ttggcccggg ccattttgca acgattgaaa aacctgcagg ccaaagtcgt tgtgaccacc
     1081 caccagagtg aactgaaaac ctttgcctac cagaatcagc gggtggaaaa tgcctgtgtg
     1141 gaatttaatc ctgtgacctt gcttccgacc tacgagttga ccatcggtac ccctggccag
     1201 agcaatgcct ttgccattgc ctcccgcttg ggcctggagc aggcaatcgt cgagcaggca
     1261 cgcagtttgg tccccgaacg ggaaagagag attggggaca tgattcgtca attgaaagaa
     1321 agccgctaca gttttgaaga aagcagccgt gaattggaga aaggccagcg tgatcttgaa
     1381 agagagcgcc gggaattatc gtctgaacgg gaaaccctgt tgaccatgaa aaatgagatc
     1441 atgcgcaaag cccgtcagga agcggacagt tatgtacgag ccatcaaacg ggaagccaat
     1501 gaagccattg acgagctgaa aggaatatta aaagaaaaag aaaagccacc caaatggcat
     1561 gaagttgagg aaaaacgcaa acgactcagg aatctgagcg tttcttttga agatgatgag
     1621 gaaggtcttg ccagtgacga tttcaaggcc ggtgattatg tacagttaaa aagcatcagg
     1681 cagtttggct atgtgttgga agcccccaac aatcagggag atgtaatggt gcaaattggt
     1741 gccacccggc tttccgttaa tcgcgaacaa ctgttgcctg cccagctcca accggaaaag
     1801 ccccagcgct atcagcagac atttgtggag aaagcccgtc atatttctcc ggaactggac
     1861 ctgcgcggca aatacgccga agaggcgctg gaagagctgg acaaatacct tgatgacgca
     1921 gctctggctg gcatcgaaaa ggtcagaatc attcacggca aaggaaccgg agcattgcgc
     1981 cgggcaatcc gcagccattt acagaaccat cattttgtca gtgaattccg cgacggcgct
     2041 attgccgagg gcggctttgg cgtaacggtg atttcgttaa aatagctttc atatcattgg
     2101 ataaaaaaga aaaggggaag gaaaaatcgc tggtgatgtt ttccttcccc ttggttgttc
     2161 tattcagctt ggttatcaat caggtcatga ccggggcctt tgtgaatctg gtgatccttc
     2221 agcggacaag gggccaaatg ctgcccggga ttgaccacga cttccttaat cattgccgga
     2281 ggacaaccac cgctttgacc gccatgagga gtgactgcct gatataattt tccttcatgg
     2341 cggggatcgg tgcatatttt gacggtgttt ttaaacagac tctgaatcgt aaagctggta
     2401 tgaatatcac aggtttcggt cggtattttg gtctcctctg cgctgccggg cttggcctgc
     2461 accatcgcct gatcataaat gtcaggacag tacttggttg ccagtttacc ggactctcta
     2521 cagatagtaa ctttctggtg aagggtacat acatctttag ggatgtattt gccaacacag
     2581 tattcgctga tgatctgctc gtccgggcaa ctggaggaag gcagcttgcc tgatttgctg
     2641 caaatactgg tcaggacaac attgtgcggc ttggcccagg gctgatattc cttgtcttca
     2701 tgcgccttct gcatcatgga ccggaacagt ttggccggat aaccgccgcc atattgattc
     2761 tgcatcgtgt atgtttcgtc ataacccatc cagacagccc cggagtataa cggcgtgagc
     2821 ccgcagaacc aacaatcatt gttgttctcc gacgtaccgg ttttgcctcc ggttggcact
     2881 cccggcacct tgccgttggt tccggtgccg ctgttggcca cagtctgcag catgctgttc
     2941 atcagccagg cggtatcggc gctcaaggca cgcttcatgg gctgtttgta cgcatagaga
     3001 acccgcccgt ggtgatccgt aattttggtg ataaaatggg gttgggcata atatccgcca
     3061 ttgccgaaac tgccataggc ggccgccatt tcaaccgggg aagccccttt ggtaagactg
     3121 cccagcgcca gcggcgacaa ccccagatca tttctcccgg gtgtattcac cagatgattc
     3181 agacccagtg ccttgcctgt ttcgtatcca ttgcttatgc caactttatc cagcaactga
     3241 atcgcatagg tgttcacaga atactgcacc gctgtccgca tggagattag gccgcggtag
     3301 ctatgatcat aatttttggg cgaccatatt tcgttattaa ccttatagga aatgggcgaa
     3361 tcatccagga cggtgtaagg catgtagcct ttttccaatg ccggcgcata gaccgtcaaa
     3421 ggtttgatgg ccgaaccagg ctggcgaaag gcactggtgg cccggttaaa accgcgctga
     3481 ttggtatact cacggcctcc cataatggcc ttgatctcac cgttgctgtg atcaaagatg
     3541 gccatgccaa cctgaatcgg attgttattc ttggtttcac tgggaaaatg ggctggatcc
     3601 ttaaaatact cttctgcatg tttttgcagg gctgcgtcca tggtggtatg gatctttaaa
     3661 ccggaactgt agatcatggc atcggcatct ttatagccct ttactttgga caagtgtttt
     3721 atggattctt cgataactgc atcgataaaa aagccataat tcatggaatt gctctgaccc
     3781 gattttaaaa cgagctcagc ctgatgggct tcttccgctt cactgtaact gataaaatgg
     3841 ctgtcacgca tactaagcag caccaaccat tgtctggctt tggctgcttc caggttttga
     3901 tagggatcat aacgacttgg gctttgcacc agtccagcca gcagggagga ttccgccaga
     3961 ttcagttcgg agatatcctt ggcaaaatat ttgttggccg ctgcctgcac gccataggcc
     4021 ccggccccaa agtagatttt attgagatag agctcgagaa tttcgtcttt ggaatacaga
     4081 gactccaatt tataggccag cagtatctcc tttacctttc gctgccaggt tttttcctga
     4141 ctgagaaaag catcgcgggc caactgctgg gtgatggtac tggcgccctg accagtcaga
     4201 tcacgggact gaagattgct cagcagggca cgggcaatgc ctttgtaatt gaccccgtga
     4261 tgattgtaga attccttgtc ctcggtggca ataaacgctt taatcaggct gtcgggaacc
     4321 ttgtccagtt caattgaggt acggtcttct tccgcatgta cgcgccagaa tattttgcct
     4381 tggtcatcat acaacagact ggcgttggca ccggtcaact gttcttcatt aaaggctggc
     4441 agcgcataag ccgaataaac gatgaccccc aaaaacaacc ctataccggc gaacagcagg
     4501 aaggaaaaaa tggttttcca tttgctgcgc gggggaggtt gggggtaatc atacttataa
     4561 tcaagataaa aatcgtccgg catgaataga acctttcttt gatataattg cgtacagacg
     4621 cttctcgttt cattattaca taacttcaaa gcgcgataaa caactttctt ttgccatatt
     4681 acgttgaaca ccgaataaac ttgcatccct gacgatactg cctgggtctg gtgaatacga
     4741 cggagaaaca actctggaag acgaacaggt aacggatgaa aaaacgggaa aggcatgtaa
     4801 ccacgcattc cccgtttttc tgcaacaaaa agccgctatt tgattcggtg ctgattgaaa
     4861 ctggcaaatg gtttagtaac gcttccctag tgatttaagc gccggcatga tatcattaat
     4921 gtgaccttca aaaaagatgg catgaccttt tacattggga taatcaagcg acatcatttt
     4981 gatgaataca aagccgattt cttccagtgt tttacggtca tagtttctga tgtagtccat
     5041 atgcttgtcc gtaatttttt tggcggtaca ataaatatcc ttgcctttgg agttgaccaa
     5101 ggcagttgct accaaagtac gcaaacgaat cacctcattt tgtaatgaaa atcccgctct
     5161 gcgaacatac cataagtatg ctcaagggcg ggatttgctc ttcctccggg ggtgctgccc
     5221 gggattattt atggtagtcc caaggggagt cgaacccctg tctccgcctt gagagggcgg
     5281 cgtcctgggc ctctagacga tgggaccata aaaatggcag ccgaacaagg attcgaaccc
     5341 tggcgaactg atccagagtc agtcgtgcta ccactacacc attgggctgc aagcgctatt
     5401 gttccgcgca aaaaatatta taatacaggg tttatataat gtcaaagggt tatggcagac
     5461 tcgcttaaaa aaaatgacga attatttaaa tgcctcaata aaagctagcg cccgggccaa
     5521 tcggcgggaa caaacctctg gtcccaacaa ttccatgatt tcaaaaaggc cgggggtatt
     5581 ggtgcgcccg gtcagtgcca ggcgggtggg atgaatcagt tcagccgctt tcaagcccat
     5641 tgcttctgcc cgggagcgga tggcctgctc aatttgtccg gcatcaaacg gttgcatgtc
     5701 cataagcgat ttaactgccg ccaatctttc ccgacaagcg gattggcgga aatacttgtc
     5761 tacacctttt tcatcataaa gctggggctc cgcaaagaaa tattcagctg caggccacaa
     5821 ttcctggata attctgatcc ggctgcgcag cagggtgatt attttgataa aataggcgtg
     5881 attatcagtc gttaaccagc cgcgcgccca cgcctcctct ttcaccctgg acaggatgtc
     5941 ttccgcgctt agttcagcta gatagtgacc gttcatccag gccagttttt cgatgtcata
     6001 gatggcagcc gatttggaga aatgggcaag atcaaagcgc cggatcattt cctctctgtc
     6061 cataaggtca agatcatcac ccggggacca gcccagcagg gccagatagt tgaccaaggc
     6121 ctgcggcagg tatccctgat cacggaattc ctgcactgaa gtggcaccat gccgcttgga
     6181 cagtttactg cgatcaggcg cgaggatcat ggatacatgg gcaaattgcg gtggagtcaa
     6241 tcccagcgcc tgataaatca gcagttgttt gggggtattg ggtagatgct cttcggctcg
     6301 gatgacatgg ctgatctgca tggtgaagtc atcaaccacc accgcgaaat tataagttgg
     6361 ccatccatct gatttggcga tgataaagtc gtcgatcaaa tcgttttgaa attccacctg
     6421 gccccgcacc aggtcatgga cagcggtgat gccttgttgc ggcatcttga agcgaaccgt
     6481 cggctttaag cccagggaga gtcgatgggc tacttcagtc gcactaaggc tgcggcaagt
     6541 accgtcatat ctgaaagcat cctggtcatg ccgggcagac tcgcgctggc ggcgcaattc
     6601 atctgccgta caaaaacagt aatacgcatg accggaatcc agcaactgtt ggagatagtg
     6661 ctgataaatg gacaaacgct cgctttgccg gtaaggacca taaggtccgc cgatatccgg
     6721 accttcatcc caatccagcc ccagccagcg caacccttcc acgatgccgt gtgcggattc
     6781 agcggtggaa cgttccaggt cggtatcttc cagacgcaac accaggctgc cctgctgact
     6841 gcgggccagc aaccaattaa acaaagcggt gcgggctccg ccgatatgta aagtccctgt
     6901 aggactgggc gcaaaacgta ctctcatctc gcccattcag ttactcctcc tttgtgaaat
     6961 gaaaacagca tgcatcctgt gagcggcagt ctgccggggg gaatgtcatc ccgaatttct
     7021 taatctatgg tagcgcatcc cggtttattt atcaaaccag gcactgtcat gacttttctg
     7081 ccgaaaaaaa gtctgtatcg ccgaatcttc agcactgctt tggatggatg gcgaaaaatg
     7141 tgcaccaagt agttctgttt ttcatatata tatatattga ttgatgaatc caaaaggcta
     7201 gtcatccggt gttatgaaca gcccccgaga tgaaaaagga ggcgcagggc ttgtattatc
     7261 gcagaagaca aaaggcagcc gtattggtat tgctgttggt tttgcttgct tcaatcgtga
     7321 tgattgactt caggattaaa aagagtctgt tggaattagc acaaacccag gcggaaattc
     7381 gtaccgttga aatcgtaacc cgggcagttt atgaacaact gggccaggca accgagtaca
     7441 atgacattgt tcatatacac aaagacgatc agggcaggat tgtaatgctg caggccaaca
     7501 cggttatttt aaaccggtac atggccctgg ccaacaaagc aattctggcc gggctcaagc
     7561 aagcgaatgc tgatcaaatc aagatcccgc tcggacagat cacgggaatc actttactgg
     7621 caggcagagg acctcgtttg ggtgtcaggg ttatgccagc cagccaggtt tttatagaag
     7681 ttgatgacag attcgaagag gcaggaatca atcaaacccg ccaccgaatt tacatgaaag
     7741 tgaaaaccag aataaaaatc gccgtgccct tgatgggcaa agaaatccta gtggtttcaa
     7801 cgattcccat gacggagaca gtaattgtgg gcgatgtgcc gcaaacctat gtcagtctta
     7861 aaggag
//
LOCUS       DAOTHZ010000085         7824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11175_length_7824_cov_6.995495, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000085 DAOTHZ010000000
VERSION     DAOTHZ010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11175_length_7824_cov_6.995495"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="P5549_08830"
     CDS             <1..612
                     /locus_tag="P5549_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW13214.1"
                     /translation="LKKAKAELPDLIILDIMLPKMDGLEVCRNVKQDNVCKTIPIIML
                     TAKGEEFDTILGLETGADDYITKPFSPRELVARVNARLRAVKLLADEKAVGAKAYVFK
                     DLTLVADKHEAFMGEVKMELTPKEFELLRLLTSNQGRVFTRELLLAKVWGYEFSGDTR
                     TVDVHVRHLRQKLHDDSNYPQYIETVRGVGYKFVEKDGPVSRR"
     gene            618..1661
                     /locus_tag="P5549_08835"
     CDS             618..1661
                     /locus_tag="P5549_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRW13215.1"
                     /translation="MLTTVQGNTVMNEILGNMAEAVLVLNGEAEVVLANRAAENLFMI
                     RDGVLIGQSIDHITDNKDVIRMTRNVLRTGKEVFTEMMLLPSTQIHRIHVSALKNEKN
                     QINGVVAVFHDVTDARNFQQMRSEFLGNVSHELRTPLTSIKGFVETLLDGAMENSDQC
                     RRFLSIIDAETYRLSRLIEDMLSLSAIESKERMMRPKPVCMLTSMRNVLNILGPQISE
                     KSLRVDLIYRNDHSYIQADEDLLNQVFINLLDNAIKFTPENGSIVIRCKKNDSRLLLT
                     ITDTGVGIPRDSLPRIFERFYRVDKARTRRQGGTGLGLSIVKHIVESHGGEVFVESEV
                     GKGSTFGVSFIAL"
     gene            1739..2665
                     /locus_tag="P5549_08840"
     CDS             1739..2665
                     /locus_tag="P5549_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRW13216.1"
                     /translation="MSKRKARNERVRTIDSFLRMLVTVAAVALAVSLVYLAGRHLLAA
                     YSKHQRDTVLAQYGVIEQKLSGEALVLRREKVFRAPAAGFFENLVNDGSKIGINTPLG
                     YYLSQGEKSTLRAAASGLFTRRIDGLEEVLQNIQLSAVGPETFAYRIQQHDPATEFKP
                     GQGVYKIVDNLAPTRLLLQFTQHQDNPALEPGQTVSLQVNDRVLGKCRVIASKQDFEK
                     LVVLVETPHFREELLHIRQAEAVLTVSSPQGYLVPEKSLLVKGKEKGIYCLNGEEVIC
                     KPVRVLAVKDGIAVVEGLQPNDLVIVNLQHIK"
     gene            2696..3373
                     /locus_tag="P5549_08845"
     CDS             2696..3373
                     /locus_tag="P5549_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRW13217.1"
                     /translation="MEELSKKLTVIQQNIVRAAGKAGRDPRDVNLLAVTKTVGIDIAR
                     QAYDLGIRDFGENRTQELNRKQAALPQARWHMIGRLQSNKVKDIVGKVHLIHSLDSWH
                     MAEEINKRALNMQTEASVLLQVNISGETQKAGVEPDEVADILASLGQLPALRLYGFMT
                     MAPLSSVGEESRPIFTELKNLQQTFQHRSFVNVDLKYLSMGMSQDYEVAVEEGAHFVR
                     IGSALFH"
     gene            3441..3878
                     /locus_tag="P5549_08850"
     CDS             3441..3878
                     /locus_tag="P5549_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRW13218.1"
                     /translation="MGLLDRFWGWLGVRDEEEQAFMELPPGGSYDKENKAAQLVSIHS
                     NKNLKVMVCEPEKFDEVQALAEHLKNRKQVILNFENTAPEISQRVIDFISGATYALDG
                     QSQQLGRNIFLFAPSNVEVTKDHRALMKKNPVSVPEGFKGDRR"
     gene            3875..4678
                     /gene="proC"
                     /locus_tag="P5549_08855"
     CDS             3875..4678
                     /gene="proC"
                     /locus_tag="P5549_08855"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRW13219.1"
                     /translation="MTWTLGVIGCGNMSYALLSGIVRSPEQAPEKIYCNDISDVRMRL
                     FADEFKALPADKGDLIAASDLIILGVKPQQIREALEDTAANWQENKLLLSIAAGIRTE
                     WLETLLSPATMRVVRAMPNTPCLAGAGMIGLCAGKSATVVDLERIKALLAASGRAVIV
                     KEQDMDAVAAVSGSGPAYVYLMIEALMEAAVSVGLSSSTARELVLQTVKGSVILLEES
                     GQHPAVLKQQVTSPGGTTIAALRQLEEGGVRRAFFNAVEAARNRSIELG"
     gene            4702..4959
                     /locus_tag="P5549_08860"
     CDS             4702..4959
                     /locus_tag="P5549_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRW13220.1"
                     /translation="MSLYNIVNVMFEVYSFLIIAQVLMSWIPHDRYHPVFRFIEEITE
                     PVLTPFRRLIPSVGGIDFSPIIALMALELVRRIILSLLAGF"
     gene            4995..6899
                     /locus_tag="P5549_08865"
     CDS             4995..6899
                     /locus_tag="P5549_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRW13221.1"
                     /translation="MSLHDLELLAPAASMEGLRAVAEAGADAVYLGGKRFNMRMLRPE
                     FNFSDEEMAEAVQYLHQLQKKVYVTINNLYYPLEIDALKTYLERLAQLQVDAFIVQDL
                     GVAQLCRENGLEIPLHASVQMGTNNAGSALLLAQNGFSRVILSRHLSLPEIEEIYRRS
                     GIGIEHFVHGETCISHCGKCLLSSFMYQESSNRGRCRKPCRWTYRMSGLDFDSADEPR
                     YYLAQHDLCLYPYLQQLIAAGVTSFKIEGRMRNQEQLALIIGCYRRALDRISDNSGSD
                     SMEEIHLLEENRIRDFCSGSLFQVPNQEDIDLLGRREPAWPYSARQLTPLGEEMLTSL
                     AETQTYAGKRIGLTVTVADQDGLQKMMMQNVDHIIISLDSMRQQHQPLELDKLKPIID
                     AHASGQTRIFIETPRIVTQKDLDMVYRLKEWAETSKLDGFVVNDLGSLHILRSQKWRL
                     WAGSGLNTANHKAAAFLQEQGIERITVSLEAGRAETEALLDERVPAEVVVQGPMQAMI
                     TDLCLIQTALEGSSTACGQECALAQPALLDEYGQAYKILTDYQCRNHIYYPLERCLYS
                     YLPALADWGVQSVRIDGQHYPPKLLTRVVYIYQQAVQENPDGLRINQEGLARLLKLFP
                     NGLTVAPFME"
     gene            6999..7499
                     /locus_tag="P5549_08870"
     CDS             6999..7499
                     /locus_tag="P5549_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRW13222.1"
                     /translation="MITGMEIRNQQFRKKMRGYDPVEVQNFVHNLAQDYENLYSENVV
                     LRENIQKVKYELDHYRKLEETMNNSLILAQQTAESIKSNAHKEAEMILEESRKKIAET
                     FLVYQEVLQRLNMITAEIKSQINSELEMLEKNQRKTEALSEFFFSKDTKEILEKLEST
                     VARDKE"
     gene            7496..7786
                     /locus_tag="P5549_08875"
     CDS             7496..7786
                     /locus_tag="P5549_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRW13223.1"
                     /translation="MTLKLTPGADGVCLEVQVQPRSSRNQVVGEQDGRLKIKLTAPPV
                     EGEANQALVNFLAGLLHIPKKNIRLLRGESSRHKLIEIMGLNEAELIAKLNI"
BASE COUNT         2057 a         1817 c         2128 g         1822 t
ORIGIN      
        1 ttgaaaaagg ccaaagcaga attgccggat ctgatcattc tggatattat gctgcccaaa
       61 atggatggac tggaagtgtg ccgcaatgta aaacaggaca atgtctgcaa gaccatcccc
      121 atcatcatgc tgactgccaa gggagaggag tttgacacca ttcttggcct ggaaacaggg
      181 gccgatgatt atatcaccaa acctttcagc ccgcgtgaac tggtggcccg cgtcaatgcc
      241 cggctgcgcg cggtcaaact attggccgac gaaaaggcgg tgggggccaa ggcctatgtt
      301 tttaaggacc tcaccctggt ggcggacaaa catgaagctt tcatgggcga agtcaaaatg
      361 gaactgaccc ccaaggaatt tgaactgctg agattgctga cctccaatca aggtcgggtc
      421 tttactcggg aattgttgct ggccaaagta tggggctatg agttttccgg tgatacccgt
      481 accgtggatg tccacgttcg gcatctgcgg caaaagctgc atgatgattc caattatccg
      541 caatatattg aaaccgtacg cggggtaggc tataaattcg tagaaaaaga cggccccgtg
      601 tccagaaggt gatgcgtatg ctgacgactg tacagggaaa tacagtaatg aatgaaatcc
      661 tgggcaacat ggctgaagct gtcctggtcc tcaatgggga ggctgaagtc gtgctggcca
      721 accgggctgc ggaaaacctc tttatgattc gcgacggcgt gctgattggc cagagcatcg
      781 atcatatcac ggacaacaag gatgttatcc gcatgacaag aaacgtttta cgtaccggca
      841 aggaagtatt taccgagatg atgctgttgc cgagtaccca aatccaccga atccatgtca
      901 gcgccctgaa aaacgagaaa aatcagatca atggagtggt ggcagttttt catgatgtga
      961 ccgatgctcg taattttcaa cagatgcgct ccgagttttt aggcaacgtt tcccatgaat
     1021 tacgcactcc gctgacctcc atcaaaggat ttgtggagac cctgctggat ggcgccatgg
     1081 aaaacagcga ccagtgccgc cggtttttat ccatcattga tgcagaaacc taccgtttga
     1141 gccgtctcat tgaggacatg ctgagcttgt cggcgattga gtccaaggaa agaatgatgc
     1201 gtcccaagcc ggtctgcatg ctgacctcaa tgcgcaatgt tttgaatatt ctcgggccgc
     1261 agatcagtga aaaatctctg cgggtggatt taatctatcg caatgatcat tcctatattc
     1321 aggccgatga agacctgctc aaccaggttt ttatcaattt gctcgacaat gccattaaat
     1381 ttacgccgga gaacggcagc atcgtcatcc gctgcaaaaa aaatgacagt cggttgctcc
     1441 tgaccataac cgataccggg gtcggcatcc cccgcgatag tctgccgcgg atttttgaac
     1501 ggttttaccg ggttgacaaa gcccgtaccc gccgccaggg agggaccggt ctgggtctgt
     1561 cgattgtgaa acacatcgtt gaatctcatg gcggtgaagt atttgtggaa agcgaagttg
     1621 gcaaaggttc aacgttcgga gtaagtttta tcgcgctgta acgcggctgc agagattgtc
     1681 atcgtccaag cttttttcga aactgtcagc gatatgaccc tccggcaggg aggaaatcat
     1741 gtcaaagaga aaggcaagga acgagcgggt acgaaccatc gattcgttcc tgcgaatgct
     1801 ggttaccgtt gctgccgtag ccctggcagt cagcctggtc tatctggccg gacgacattt
     1861 gctggctgcc tacagtaaac atcagcggga taccgtattg gcccaatatg gggtcattga
     1921 acaaaaattg tcgggagagg ctttggttct gcgccgggaa aaagttttcc gggcccctgc
     1981 tgccggcttt tttgaaaatc tggtcaacga cggcagcaag atcggtatca atacaccgct
     2041 gggatactat ctgagccagg gtgaaaagag caccctgcgg gctgctgcca gcggcttgtt
     2101 tacccgccga attgatggcc tggaagaagt gttgcagaac attcagctat cggctgttgg
     2161 cccggagacc tttgcctatc gtatacaaca gcatgatcct gcgacggaat tcaaacccgg
     2221 gcagggtgta tataaaattg tcgacaatct ggcaccgacc cgtttgctgc tgcaattcac
     2281 gcagcaccag gataatcctg ccttggaacc gggtcagacg gtcagcctgc aggtgaacga
     2341 ccgggtcttg ggaaaatgcc gtgtaattgc cagcaagcaa gattttgaga aactggtggt
     2401 tttggttgaa acccctcatt tcagagaaga gttgttgcat atccggcaag cggaagcagt
     2461 gttgaccgtg tcttcgcccc agggttatct ggtgccggag aaatctctgc tggtcaaggg
     2521 taaggaaaaa gggatatatt gtctcaatgg cgaagaagta atctgcaaac cggtacgggt
     2581 tctggcggtc aaagatggga ttgccgtggt tgaaggtctt cagcccaacg acttggtgat
     2641 tgtcaatctg cagcatatta aataaacatc gttgaagcaa ataacaggag acaacatgga
     2701 agaactaagc aaaaaattga ctgtaattca gcagaatatt gtcagagcag ccgggaaagc
     2761 cggacgcgat ccccgcgatg ttaacttgct ggcagtgacc aagacggtcg gcatcgacat
     2821 cgcccgacaa gcgtatgact tggggattcg agatttcggt gaaaaccgta cccaggaact
     2881 gaaccgcaaa caggccgctc tcccgcaagc ccgctggcat atgattggga gactgcagtc
     2941 caataaagta aaagacattg tcggcaaggt gcatttgatt cattccttgg acagctggca
     3001 tatggccgaa gaaatcaaca aaagagcgct aaatatgcaa accgaggcat cagtattgct
     3061 gcaggtaaat atttccgggg aaacacagaa ggccggggtt gaaccggacg aggtggcgga
     3121 catccttgct tccctaggcc aactgccggc cttgcggctg tacggcttta tgaccatggc
     3181 accgctttcc tctgtcggag aagaaagccg gcccattttc actgaattaa aaaatctcca
     3241 acaaacattc cagcatagat cttttgtcaa tgttgattta aaatatctat ccatggggat
     3301 gtcgcaggat tatgaagtcg ctgtcgaaga gggtgcccac tttgtacgca ttggcagcgc
     3361 cttgtttcat taggatagat aggtgaatat cagagatcaa gcagcaggaa aaaacgaaaa
     3421 attttgagga ggctacaaca atgggcttac tggatcgttt ttggggatgg ctgggagtac
     3481 gggatgaaga agaacaagcg tttatggaat tgccgcccgg cggcagctac gacaaggaaa
     3541 acaaggcagc ccaactggtt agtatccaca gcaacaagaa tctcaaggta atggtttgtg
     3601 aaccggaaaa atttgatgag gtacaggcgt tggctgagca tttgaaaaac cgtaaacagg
     3661 tcattttgaa ttttgagaac acagccccgg aaatctctca gcgggtgatt gatttcatca
     3721 gcggtgccac gtatgccctg gatggacaaa gccagcaact gggacgcaat atcttcctgt
     3781 ttgcaccctc caatgtggaa gttaccaaag atcatcgggc actgatgaag aaaaacccag
     3841 tatcggttcc ggagggattc aagggggaca gaagatgacc tggacgctgg gagttattgg
     3901 ctgcggcaat atgtcctatg ctttgttaag cgggattgtc cgcagtccgg aacaggctcc
     3961 cgaaaaaatt tactgcaatg atatcagtga cgtgcgcatg cgcttgtttg ctgatgagtt
     4021 caaagccttg ccagccgaca aaggtgattt gatcgcagct tccgatttga tcattctagg
     4081 ggtcaagccc cagcaaatcc gtgaagcgct ggaggatact gccgctaact ggcaggaaaa
     4141 caagctgttg ctttcgattg cagccggtat tcgtaccgaa tggctggaaa cattgttatc
     4201 gccggcaacc atgcgagtcg tcagagccat gcccaataca ccttgtctgg cgggcgcagg
     4261 catgatcggt ttgtgtgccg gcaaatcagc cacggttgtt gatctggaaa ggataaaagc
     4321 gctgttggcc gccagcggtc gggcagttat cgtcaaagag caggacatgg atgcggtggc
     4381 ggctgtatcc ggcagcggac ctgcgtatgt atatctgatg atcgaagccc tgatggaagc
     4441 ggcggtaagt gttggcttaa gcagcagcac tgcccgggag ctcgtcttgc agacggtgaa
     4501 aggcagcgtg attttgctgg aggaaagcgg tcagcatccc gccgtgctca aacaacaggt
     4561 gacttccccg ggaggaacca ccattgccgc cttgcgccaa ctggaagaag gcggggtccg
     4621 acgggctttt ttcaatgcgg ttgaagcagc ccgcaaccga tccatcgaac tcggataata
     4681 aagcgtagga ggaaatcatt cttgagtttg tataatatcg tcaatgtcat gtttgaagtt
     4741 tattcatttc taatcattgc ccaggtattg atgtcctgga tcccccatga tcgttatcat
     4801 cctgtatttc gttttattga agaaattacc gaaccggtcc tgacaccatt ccgcagattg
     4861 ataccgtccg tgggggggat tgatttttca cccattattg ccttgatggc acttgaactg
     4921 gtgcgccgca tcatcctgtc cctgttggcg ggtttttagg catggaaagc tgagtgatta
     4981 aggagaatag atgtatgtcg ctgcatgatc tggagttatt ggctccggcc gcttccatgg
     5041 agggattgcg agcggtggcc gaagctggag ctgatgccgt ttatctgggg ggcaaacgtt
     5101 tcaatatgcg gatgctgcgc ccggaattta atttttctga tgaagaaatg gctgaggctg
     5161 ttcaatatct gcaccagcta cagaaaaagg tctatgtcac catcaacaat ttatactatc
     5221 cgttagaaat cgacgcgttg aaaacatatc tggagcgttt ggcacagctg caggtggatg
     5281 ccttcatcgt acaggatttg ggggtggctc agctttgccg ggagaacgga ctggaaatcc
     5341 cgctgcatgc cagtgtacag atgggaacca acaatgccgg ctccgccctt ttgttggcac
     5401 aaaacggttt cagccgggtt attctgtccc gccacttgag tttgcccgag attgaggaga
     5461 tctatcgccg cagcgggatc ggcatcgaac actttgtcca tggagaaacg tgtatttcgc
     5521 attgcggcaa gtgcctgctg agcagtttta tgtatcagga gagttccaac cggggacgct
     5581 gccgcaaacc ttgtcgttgg acctatcgaa tgtccggatt ggattttgat agcgcagatg
     5641 agccgcggta ttatttggcc cagcatgatt tgtgcctcta tccctatttg cagcagctga
     5701 ttgctgccgg ggtgacgtca ttcaagatag aaggcagaat gcgcaatcag gagcaattgg
     5761 ccttgatcat cggctgttac cgccgggcat tggatagaat cagcgataat tccggaagcg
     5821 attcgatgga agaaatccat ttgcttgaag aaaaccggat ccgtgacttt tgcagcggta
     5881 gcctttttca ggtacccaac caagaagata ttgatttgct aggtcggcgg gaacctgcct
     5941 ggccatattc tgcccggcaa ctgacgccgc tgggggaaga aatgctgact tctctggcag
     6001 aaacacaaac ctatgccggc aagcgcatcg gattgaccgt taccgttgcc gatcaggatg
     6061 gcttgcaaaa gatgatgatg caaaacgtgg atcacatcat catcagtctg gacagcatgc
     6121 gccagcagca ccaaccgctt gaactggaca aactgaaacc gatcattgat gcccatgcaa
     6181 gcggacaaac gcgcattttt atcgagacgc cgcggatcgt tacccaaaaa gatctggaca
     6241 tggtttatcg tttaaaagaa tgggcagaaa ctagcaagct ggacggtttt gtcgtcaacg
     6301 atttgggcag tttgcatatc ctgcgctcgc aaaaatggcg tttgtgggct ggaagcggat
     6361 tgaatacagc caaccataaa gccgccgctt ttttacagga gcagggaata gaacgtatca
     6421 ccgtttctct ggaagccggg agagcggaaa ccgaagcact gctggatgaa cgggtgcctg
     6481 ccgaagtcgt tgtacagggc ccgatgcaag ccatgatcac cgacctgtgc ttaatccaaa
     6541 ctgccctgga aggcagcagc actgcctgtg gacaggaatg cgccctggcc cagccggctt
     6601 tgctggatga atacggtcag gcttataaaa tcctgaccga ttatcaatgc cgcaatcata
     6661 tctattatcc gctagagcgg tgtctgtact cctatctgcc agcactggcc gactggggcg
     6721 tacaatccgt gcgcattgac gggcagcatt acccgcccaa acttttgacc cgggtggttt
     6781 atatttatca gcaggcagtg caggaaaatc cggatggatt gcgtatcaac caagagggac
     6841 tggccagatt gcttaagttg tttcccaacg gcttgaccgt tgctccgttt atggagtaaa
     6901 cgatgctgca aagccatgca ggaaatttta ttagaaaaca gcctgatgct ggcggcacaa
     6961 cagatgatgg agatagattt ttgaaaggag atgggtagat gattaccggc atggagattc
     7021 gcaatcagca attccgcaag aaaatgcgcg gctatgatcc tgtggaagtt cagaatttcg
     7081 tccataatct ggcgcaggat tatgaaaatc tctacagcga aaacgttgta ctgcgggaaa
     7141 atattcaaaa agtcaaatat gaactggacc attatcgcaa actcgaagaa accatgaaca
     7201 acagcctgat cctggcccag cagaccgcgg agtcaataaa aagcaatgcc cacaaggaag
     7261 cggagatgat cctggaggaa tcccgtaaaa aaattgccga gacgtttctc gtctatcagg
     7321 aggtcctgca gcgactcaat atgatcaccg ccgaaattaa atcccaaatc aacagtgaac
     7381 tggaaatgct ggaaaaaaac cagcgcaaga ccgaggcact cagtgaattc tttttcagca
     7441 aggataccaa ggaaattctt gaaaaactgg aaagcaccgt tgcgagggat aaagaatgac
     7501 actgaagctc acccccggcg ctgacggtgt atgtctggaa gttcaggttc agccgcgttc
     7561 ttcccgcaat caggtggtgg gtgaacagga tgggcgcctt aaaatcaaac tgaccgcgcc
     7621 gccggtggaa ggtgaagcca accaggcgct ggtcaatttc ctggctgggc tgctgcacat
     7681 ccccaaaaag aacatccggt tgctgagagg cgaatcttcc cgccataagt tgattgaaat
     7741 catgggcctg aacgaagcgg agctcattgc caagctaaat atataaacag cggatgccgg
     7801 gttggacatc cgctgttttt ttag
//
LOCUS       DAOTHZ010000086         7747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11302_length_7747_cov_7.010270, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000086 DAOTHZ010000000
VERSION     DAOTHZ010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11302_length_7747_cov_7.010270"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="P5549_08880"
     CDS             complement(<1..706)
                     /locus_tag="P5549_08880"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRW13224.1"
                     /translation="MMSRRAIIIVMDSVGIGAMEDSYLYGDEGSNTIVNTARAVGGLH
                     LPNLQAMGLGNLAEIEGVPAVAQILGAAGIMREKSKGKDTTTGHWEMMGIIQEHPFPT
                     YPHGFPEDLIKEFERRIGRSILGNVVASGTEIIKELGEAHLATGFPIVYTSADSVFQI
                     AAHEEVIPLEELYRYCRIAREMLQGEHNVGRVIARPFIGQAGAFVRTANRHDFSLLPP
                     DNVLDRIVAAGMKVAGI"
     gene            complement(748..1641)
                     /gene="xerD"
                     /locus_tag="P5549_08885"
     CDS             complement(748..1641)
                     /gene="xerD"
                     /locus_tag="P5549_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRW13225.1"
                     /translation="METYITAFLAYLGLEKGLTANTQAAYRRDLHKFLTYLQKQRSGI
                     INWEEVSKRDIISFLAGQMDEGAAYSTVARSLSSIKSFYKFMVMEDHLAVNPTTDLET
                     PKINRKLPKVLSIEEVDKLIEQCNVMLPLGLRDRAMLELMYGTGLRVSELLSLQMDDI
                     NFTAGFLRCFGKGRKERIVPVNNSSITWIQRYITRTRRELLRNQYERTLFLNAHGRAM
                     TRQGFFKILAGYAEKSGIEKEITPHTLRHSFATHLLENGADLRAVQEMLGHADISTTQ
                     IYTHITRSHLREVYHQCHPRA"
     gene            complement(1698..1982)
                     /locus_tag="P5549_08890"
     CDS             complement(1698..1982)
                     /locus_tag="P5549_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13226.1"
                     /translation="MIIVVGEWRKKVVKIVAAVIVVGLFAAAIPFVSGILYDKVPVFT
                     GWFDDEHPSGNPLRVDNNTTGVADRTESVFNEQMDRFVVKVQNFYYEEKE"
     gene            complement(2153..2797)
                     /gene="spoIIM"
                     /locus_tag="P5549_08895"
     CDS             complement(2153..2797)
                     /gene="spoIIM"
                     /locus_tag="P5549_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRW13227.1"
                     /translation="MAAIQAGKGWVMNLIGTIINHIKANRWQYLLISIIFLTGLISGS
                     YRADYLDGAVRSHLLNLVDNYLRGSSQGDLAGSQLLFYAFLNQTKTIVLIWFLGLTVI
                     GMPLILGICFLKGYSLGFTVGFLVQERAGVGILISLLAILPQHLLYIPLLIIWSVAAI
                     KFSVAITVGRNASALSMGRALLAFTAQMLLFLLFVLAGALIEAYFSPWFIQIFV"
     gene            complement(2988..4340)
                     /locus_tag="P5549_08900"
     CDS             complement(2988..4340)
                     /locus_tag="P5549_08900"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRW13228.1"
                     /translation="MKPVYVIGHKNPDVDSVAAAISYKVYKQSTDKGVYIAAAAGELK
                     DELKKLLDHLEFEHPMILSNVGTTVEDLLEEERSLYINADTTLVELGNIMRNNKLKTV
                     PVLDEKQKFLGLITIGDVAMIYLEALGTGRDIDNSPEILRQILAKKASAIMKSRNLVL
                     FEKDEKAEEAKKMMLSSRFRNYPVVDDDNRYLGMISRYNLLQMKRKQVILVDHNEKKQ
                     AVEGIEEAEILEIIDHHRVGDLQTIFPIYFHNEPVGSTSTLVADKFLTNKIFLANNLA
                     ALLLSGIMTDTMIFKSPTTTAKDKRVAQELQQICGLDPVEWGKKLFAESSRVDKQSNL
                     ELISEDLKEYVSGTTMFAISQVETIDLDQFAERRTDLVATMENLCTQNGYALMCLMVT
                     DIFEEGTELIVAGEMSALVEEEFQHDHKQGGIFLKGVLSRKKQVVPVIYEMLRKQMMM
                     "
     gene            complement(4434..5144)
                     /locus_tag="P5549_08905"
     CDS             complement(4434..5144)
                     /locus_tag="P5549_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13229.1"
                     /translation="METLRRIIVESDASGAREAARAGLMVMIVDVIDMSTTLESALDA
                     GAAAVLGASPDFTRAPVKVMPEEIGRHAARLAREAGQGIIVVAEPRVGSDEERLARCQ
                     KLMKGIESERGMVEAVIPNIGAETPKLADFKNKIVVAVSDTGGVAYDAAFTEKGRVLT
                     GTVARTYSQKGIQPALSAVQRARSMFTAQDKGLAIIAASRNSLEDVLAAQFIANLFLQ
                     ECYAGDVLSVTKFVNDPV"
     gene            complement(5168..5698)
                     /locus_tag="P5549_08910"
     CDS             complement(5168..5698)
                     /locus_tag="P5549_08910"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRW13230.1"
                     /translation="MDFKEETISSREIFKGRIIQLKVDTVRLPNARESTREIVEHAGA
                     VAIIALDEEENIIMVKQYRKPVEQVLLEIPAGTLEAEEDPLDCARRELREETGLTADH
                     WQKILSYYSAPGFCNEQLHLYLASGLHEGQDDTDEDEFVEMIRLPLQDAYQMIFTGEI
                     QDGKSIIGVQYAWHKR"
     gene            5761..5886
                     /locus_tag="P5549_08915"
     CDS             5761..5886
                     /locus_tag="P5549_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13231.1"
                     /translation="MEMMIQNNAVCFYGQIKDLPGFLSAWPAELTLAEFIRLHLN"
     gene            complement(5973..7073)
                     /locus_tag="P5549_08920"
     CDS             complement(5973..7073)
                     /locus_tag="P5549_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HRW13232.1"
                     /translation="MIQSQRAKIIKEIFSRPGIKGFLAEVQGREEKCIAYSALTEEIL
                     PGDEVLLNTTAMALSLGSGGYHYIISKVEPAGRELTPGGHIMKLRYTPMQIKVLAVEE
                     EDSPWHATMAEADSLDGIPVLAATLHSMLAPICVSIASQANLRVAYVMTDGAALPIAF
                     SQNVAWLKEQGLLVGSVTCGHAFGGDLEAVNIYSALLAARHVLKADVIIVSMGPGIVG
                     TGTKWGFTGVEQGDILNAVDSLKGVPVAVPRISFADARERHLGISHHSLTVLSHICKV
                     PVLVPLPLLPDPQSDYMMQQLRGTKLLEKHKICMEDATGCLAVMQNSGLKMTTMGRSL
                     DADREFFLSLGAAALTAVRLAHGQAPHYLHQA"
     gene            complement(7084..>7747)
                     /locus_tag="P5549_08925"
     CDS             complement(7084..>7747)
                     /locus_tag="P5549_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRW13233.1"
                     /translation="QAEIAYALDSGGSPGTIVVKSPCQNEISYSVQGKAAHAGINPED
                     GINAIQIMAKALAAMPCGRLNPTTTCNFGIIKGGEARNIVAEHCWVKGEARSHARTQL
                     EQLTGELTGTFKEVVQKNGGEPEVVVKFLYPEISLDEDEKVVVLAQKAAQNLGLEVNL
                     ISTGGGSDAAIVNGNHIRCANLGTGMSSVHTSDEYISLKDLVNDAAWVLAIIQQYWAD
                     VS"
BASE COUNT         1799 a         2186 c         1757 g         2005 t
ORIGIN      
        1 cgattccggc taccttcatg ccggcggcaa cgattcgatc gagcacattg tccgggggca
       61 ataaagaaaa gtcatgccgg ttggcagtac ggacaaacgc cccggcctgt ccgataaaag
      121 gacgggcaat gacccggcct acattgtgtt ccccctgcag catttcccgg gcgatccggc
      181 agtaacgata caattcttcc agagggatga cctcttcatg ggcagcaatc tgaaagacgc
      241 tgtcggccga cgtatagaca atgggaaagc cggtggccag atgcgcttcg cccagctcct
      301 tgatgatttc ggttccggaa gccactacgt tacccagtat cgaacggcca atgcggcgtt
      361 caaattcctt gatcaaatct tcggggaagc cgtgcggata agtcggaaaa ggatgctcct
      421 gaatgatccc catcatctcc cagtgtccgg tcgtggtgtc cttgcccttg gatttttccc
      481 gcataatgcc ggccgcacct agtatttggg caacagccgg aaccccctca atctcagcca
      541 gattgcccaa tcccatggct tgcaggttgg gcagatgcag accgccgacg gcccgggcgg
      601 tattgacgat ggtattgctg ccctcgtctc catacaaata gctatcctcc atggctccga
      661 tcccgacact atccatgaca atgattattg ctctccttga catcataaaa tcctccttca
      721 tttctatatt gatggcccat tcattgctta ggcacgcgga tggcactggt gatagacctc
      781 ccgcaagtga ctgcgggtga tatgggtata gatttgggtc gttgaaatat cagcatgtcc
      841 cagcatttcc tgcacggctc tcagatcggc gccgttttcc agcaggtggg tggcaaacga
      901 gtgccgcagt gtatgcgggg taatctcttt ctcaataccc gatttttcag catatccggc
      961 caaaatttta aaaaagccct ggcgggtcat ggcccgtcca tgggcattca gaaacagcgt
     1021 ccgctcatac tgatttctca acagttcgcg gcgggttctg gtgatgtagc gctgaatcca
     1081 ggtaatggaa ctgttattga ccggaacgat gcgttccttg cggcctttgc cgaaacagcg
     1141 cagaaaaccc gcagtaaaat tgatatcatc catttgaagg gacaacaact cagatacccg
     1201 cagtccggta ccgtacatca attccagcat agcccggtcg cgcaagccca acggcaacat
     1261 cacattgcat tgttcaatca gtttgtccac ttcttcgatc gataaaacct tgggcagctt
     1321 gcggttaatt ttaggtgtct ccaaatcggt cgtcggattc acggccagat gatcttccat
     1381 gaccataaac ttgtaaaagc ttttgatgct ggataaactg cgggcaaccg ttgaataggc
     1441 tgccccctca tccatctgcc cggccaggaa actaatgatg tcccgcttgc tgacctcttc
     1501 ccagtttatt atacctgacc gctgcttttg cagatacgtc aagaatttat gcaaatctcg
     1561 gcgataggct gcctgggtgt tggccgtcaa tcctttttcc agccccaaat aggccaaaaa
     1621 cgctgtaatg tatgtttcca tagccagaac tcctgcattt tctttcatta tatgacaagc
     1681 cggcgtttta tgcctgccta ttctttttct tcataataaa agttttgcac tttcaccacg
     1741 aagcgatcca tttgctcatt gaaaacactt tccgtccggt cagcgacacc cgtcgtatta
     1801 ttatccaccc gcaagggatt gccgctggga tgttcgtcat caaaccaccc ggtaaagacc
     1861 ggcactttgt cataaagtat cccggaaacg aatggtatgg cagcggcaaa caaccccacc
     1921 acaatgaccg cagccacgat tttaaccacc ttttttctcc actcacccac tacaataatc
     1981 atttaattgc ctcctcccct tatttccatt ttgtatctga tatgcatgtt acaggcctgc
     2041 cagcccgttt cgtacccctg cgtttgcgac aaaagcaaaa ggttcgccgg taatttttat
     2101 cctgttgtat gttcagatgc gaatttttca tctttgtttg acaatgcctt gctcaaacaa
     2161 agatttgaat aaaccagggt gaaaaatatg cctcgataag cgcccctgcc aacacaaaca
     2221 gcaaaaacaa cagcatttgc gcggtgaaag cgagcagcgc tctgcccatc gacaaggcgc
     2281 tggcatttct gccgacggtg atcgctaccg agaacttgat ggcagctaca gaccagataa
     2341 tcaacaaggg aatatacagc agatgctgcg gtaaaatggc cagcaagctg atcaggattc
     2401 caaccccggc tctttcctgt accaggaaac ccaccgtgaa tcccagggaa taacctttca
     2461 ggaagcaaat tcccagaatt agcggcatgc cgatcacggt caggccaaga aaccaaatca
     2521 ggacaatggt tttggtttga ttcaggaacg cataaaacaa cagctggctg cccgccagat
     2581 ccccttgact gctgcctcgc aaataattgt cgacaaggtt cagcagatgg ctcctgacgg
     2641 ctccatccag ataatctgcc cggtaactgc cgctgatcaa acctgtcagg aaaatgattg
     2701 aaatcagcaa atactgccag cggttggctt tgatgtggtt tatgatagtg ccgatcagat
     2761 tcatgaccca accctttcca gcctgaattg ctgccaagcc tccctactca ttcatatttt
     2821 gacccagtct ggtttatgcc cattaagaaa ataaacaaaa tgatttttgg ggacagtgtc
     2881 caaaaatcaa taacaaaaag gatggcacag gccgtgcggc ctgcgccatc cttaattgcg
     2941 aggattgcta atacttcaat tcccgtacct ctccaggcaa tgaaatgcta catcatcatt
     3001 tgtttgcgca gcatttcgta aattaccgga accacttgtt tttttcggga caggactcct
     3061 ttcaggaaga tcccgccttg cttgtggtca tgctggaatt cctcctcgac cagcgcactc
     3121 atttcaccgg ccacgatcag ttccgtgcct tcttcgaaaa tatcggtgac catcaggcac
     3181 atcaaggcat atccgttttg cgtgcagaga ttttccatgg tcgccaccag atccgtgcgg
     3241 cgctcggcaa actgatccaa atcgatggtc tcgacttggg aaatggcaaa catagtggtt
     3301 ccactgacat attccttcag gtcttcgctg atcaattcca gattgctctg cttgtccacc
     3361 cggctgctct ccgcaaataa ttttttaccc cactccactg gatcgagtcc gcagatttgt
     3421 tgcagttcct gggcgactct tttatccttg gcggtggtgg tgggtgattt aaaaatcatg
     3481 gtatcggtca tgatccctga caacaacagc gccgccagat tgttggccaa aaagatttta
     3541 ttggtcagaa atttatccgc gaccaaggtg gacgtcgagc ctaccggttc attatgaaaa
     3601 tagatcggga atatggtctg cagatcgccg acccgatgat ggtcaatgat ttccaggatt
     3661 tcggcttcct caataccttc cacggcctgt ttcttttcat tgtgatccac cagaatcacc
     3721 tgtttgcgct tcatttgcag cagattgtaa cgggaaatca tgcccagata gcggttgtcg
     3781 tcatcgacca ccggataatt gcgaaagcgg ctggagagca tcattttttt tgcttcctcc
     3841 gccttttcat ctttctcaaa caagaccaga ttcctcgact tcataatggc agacgccttc
     3901 ttggccagaa tctggcgcag gatctccggg ctgttgtcga tatcccggcc ggtgcccagc
     3961 gcttccagat agatcatagc cacatcaccg atggtgatca ggcctaaaaa cttctgtttt
     4021 tcatccagca ccggcacggt ctttaatttg ttattgcgca tgatattgcc cagttcaacc
     4081 agggtcgtat ccgcattaat ataaagggaa cgttcctcct ccagcagatc ctctacagtc
     4141 gtccccacat tggataagat catcgggtgc tcaaattcca aatgatcgag caattttttt
     4201 aattcgtcct ttaattctcc ggctgccgca gcaatataaa cacctttatc agtgctctgt
     4261 ttgtacactt tatagctgat cgccgccgcc accgaatcca catccggatt tttatggcca
     4321 ataacatata ccggtttcat ccaatccctc ccctgttaca ccgtgttaca ccgggacgtt
     4381 tttagtgtaa cacctaaaat acgcaaaaat ttgttcaaac gctaaagaaa taattacacc
     4441 ggatcgttaa cgaactttgt tacagaaaga acgtccccag cgtaacactc ctgcagaaac
     4501 aggttggcta taaactgggc tgccagtaca tcctccaggg agttgcggct ggcggcaatg
     4561 atggccagcc ctttgtcctg ggcggtgaac atgctcctgg cgcgctgtac ggcggacagc
     4621 gccggctgga ttcccttctg gctataggta cgggccacgg taccggtcag cacccgtccc
     4681 ttctcggtaa aagcagcatc ataggccacc ccgccggtat cggaaacggc gactacgatc
     4741 ttgtttttaa aatcagccag tttgggtgtt tccgcgccaa tattgggaat gacggcttcc
     4801 accatgcctc gttcactttc gattcccttc atgagcttct ggcagcgggc cagacgctct
     4861 tcgtcacttc ccacccgcgg ctcggccacc acaatgatcc cctgcccggc ctcccgggcc
     4921 aggcgggcgg catgccgacc aatttcctcc ggcatcactt tgaccggggc gcgggtaaaa
     4981 tcagggctgg cccctaaaac ggctgccgct cctgcatcaa gcgcactttc cagcgtagtg
     5041 gacatatcaa tgacatcgac aatcataacc attagacctg cccgggctgc ttcccgggca
     5101 ccgctggcgt cactttcaac gatgatcctg cgcagtgttt ccatccctgt tctcctcccg
     5161 ccactgatta tcttttatgc caggcatact gcacgccaat aatgcttttg ccatcctgga
     5221 tttccccggt aaaaatcatc tgataagcat cctgcagtgg taaacgaatc atttccacaa
     5281 attcatcctc atcggtatca tcctgcccct catgcagtcc gctggccaga taaaggtgca
     5341 attgttcatt gcaaaaaccg ggggcactgt aataggatag tatcttttgc caatggtcgg
     5401 cggtcaggcc ggtttcctcc cgcaattcac gccgggcaca gtccagcgga tcttcctctg
     5461 cttccagggt tccggcgggg atctccagca acacttgctc aaccggtttg cggtattgct
     5521 tgaccatgat gatattctcc tcttcatcca gcgcaataat agccacggca cctgcatgct
     5581 ccacaatctc ccgggtactt tccctggcat tgggcaaacg gactgtatca acttttagct
     5641 ggatgattct gcccttgaag atctcccggc tgcttatggt ctcttcttta aaatccatct
     5701 tatttcctcc aggtatattt ttccatcaaa tgcatatctt atggacgagg tgatgtttac
     5761 atggaaatga tgattcagaa taacgctgtc tgtttttacg gtcaaatcaa agacctgccg
     5821 gggtttctat cggcctggcc ggcggagttg accctggcgg aattcatccg tctgcatcta
     5881 aattaaagat tttgcagcat ttacgtaact cactgacgct cggaatgcaa tgctcgcctt
     5941 tcgatcgctt tcatcaattg attgctgctt gttcacgcct ggtgcagata atgaggagcc
     6001 tggccatgcg ccagccggac cgccgtcagg gcggcagcac ccagacttaa aaagaattcc
     6061 cggtctgcgt ccaggctccg tcccatggtg gtcattttaa ggccgctgtt ttgcatcaca
     6121 gccaggcaac cggtggcatc ctccatacaa attttatgct tttccagtaa tttggtgccg
     6181 cgcagctgct gcatcatata gtcactctgt gggtctggta aaaggggtag tggcaccagt
     6241 accggcacct tgcatatgtg actcaaaaca gtcaaactgt gatgactgat gccgagatgc
     6301 cgttcccgtg catcggcaaa actgatgcgc ggcacggcca ctggtacacc tttcaaacta
     6361 tcgaccgcat taaggatatc cccctgttca actccggtaa atccccattt ggttcctgta
     6421 cctacgattc ccggtcccat gctgacgata atcacatctg cttttaaaac atgacgagcc
     6481 gccaacaagg ctgagtaaat attgaccgcc tccagatcgc cgccgaaggc atgaccgcag
     6541 gtcacgctcc cgaccaacag tccctgttct ttcagccagg ccacattttg gctgaaagca
     6601 atgggcaggg ctgccccatc cgtcattaca taagctaccc gcagatttgc ctgactcgct
     6661 atactgacgc atattggcgc cagcatggaa tgcagggtag ccgccagcac cggaatgccg
     6721 tctaaactgt cggcctcggc catcgtcgca tgccagggac tgtcctcttc ctctaccgcc
     6781 aatactttta tttgcatagg cgtatagcga agtttcatga tatgcccgcc gggagtcagt
     6841 tcacgccccg cgggctcgac tttgctgata atataatgat aaccgccgct gcccagcgac
     6901 aaagccatgg cggtcgtatt gagcagcacc tcgtccccgg gcagaatctc ttccgtcaag
     6961 gcactatagg cgatgcattt ttcctcccgt ccctgcacct cagccagaaa gcctttgatg
     7021 ccagggcggg aaaagatttc tttgatgatt ttggcccgtt ggctttgtat catatcgcct
     7081 ctcctaactc acgtccgccc aatattgctg gatgatggca agaacccatg cagcatcatt
     7141 caccaaatct ttgaggctaa tatactcatc gctggtgtgg acactgctca tcccggtgcc
     7201 caggttggca cagcggatgt ggttgccgtt tacgatggcc gcatcgctgc cgccgccggt
     7261 actgatcaga tttacctcca agcccaggtt ttgggctgcc ttttgggcca gaacaaccac
     7321 tttttcatcc tcgtccaagc ttatttccgg ataaagaaat ttcaccacaa cttcgggttc
     7381 tccgccgttt ttctgtacga cttccttaaa cgtgccggtc agttctccag tcaattgttc
     7441 caattgcgtc cgcgcatgac tgcgggcttc ccctttgacc caacagtgtt ccgccacaat
     7501 attgcgggct tcgccgcctt ttataatccc aaagttacag gtggtggtcg ggtttaatcg
     7561 tccacagggc atggctgcca gcgctttcgc catgatttga atggcattga taccatcttc
     7621 cggattgatt ccggcatggg ccgccttgcc ctgcaccgaa tagctgattt cattttggca
     7681 gggtgatttt accacaatcg taccgggcga tccgccgctg tccagggcat aggcaatctc
     7741 ggcctgc
//
LOCUS       DAOTHZ010000087         7612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11565_length_7612_cov_7.626704, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000087 DAOTHZ010000000
VERSION     DAOTHZ010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11565_length_7612_cov_7.626704"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /gene="dapF"
                     /locus_tag="P5549_08930"
     CDS             <1..1978
                     /gene="dapF"
                     /locus_tag="P5549_08930"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRW13234.1"
                     /translation="EALQAANKLEYPVLMRPSYVLGGQNMIIAFSDDDIREYMDIILA
                     YNIENPVLIDKYLMGIEIEIDAICDGEDILIPGIIEHIERAGIHSGDSIAVYPAWNLS
                     GELIEQVIDDSKKLALALDTQGLVNIQYVIHGGEIYVIEVNPRSSRTIPYISKVTGVP
                     MVDLATRAMLGEKLRDMGYGTGLYKTPPYVAVKVPVFSFEKLMDVDVALGPEMKSTGE
                     VLGIGKTLAEALYKGLVAAGYKMVKQGGVLITVRDTDKPEIVDIARQYAELGFTLYAT
                     GGTANLLNQAGLPAISVKKIHEADHDNIQTLLESGRINYIISTSSKGRLPGRDSVKIR
                     RKAVESAIPCLTSLDTASALANSLKSRYSQSNTELVDINHMRNERMRLAFTKMQSCGN
                     DYIYFDCFDQEIVSPESLSVYLSDRHYSIGSDGIVLICPSEIADAKMKMFNLDGSESG
                     MSGNALTCMGKYIYENNIAVKDQITIETLSGIKKLKLYVKNEKVHSVCIDMGPAELRH
                     EKIPVYLDGQQIIDRPVNIAGREYRITCVSMGNPHCVVFDESLASIDMDEVGPAFEYS
                     MLFPERVNVEFVTVIDQNTIKMRVWERGNGETLSTGTSACAGVVAAVLNGWCRKDQHI
                     LVKQKGGNLIVKYTDDTVFMTGKPETIFAGTVEI"
     gene            2297..2635
                     /locus_tag="P5549_08935"
     CDS             2297..2635
                     /locus_tag="P5549_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRW13235.1"
                     /translation="MKKIEAIIRPAKLEELKTALEAVGVRGMTVYDVKGRGLQCAEKQ
                     YYRGQEHSIDLFPKVKLEIVCQDESVENIISQIIKTCKTGKKGDGKIFVYPVERIIRI
                     STREEGCAAL"
     gene            2788..4128
                     /locus_tag="P5549_08940"
     CDS             2788..4128
                     /locus_tag="P5549_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRW13236.1"
                     /translation="MKTKSKWLSMLTILAAFILGLPAVAFAADAPTMNSGDTAFMIIC
                     AALVMIMTPGLAFFYGGMVRKKNVMGTIMQSFAALALVSVQWVLLGYTLSFGPDMHHL
                     LGGLSFLGLSGVTGDPSANYANTIPHFVFVFYQLMFAIITVALISGAFAERMRFSIFV
                     AFVLLWTTLVYDPLAHWVWAADGWLFNLGALDFAGGTVVHISSGISGLVASILLGRRN
                     KSGAEPMIPHNLPFTMLGAALLWFGWFGFNAGSALAANGLAASAFLVTNTCAAAGAIG
                     WAAAEWLQHGKPTTLGVASGLVAGLVAITPAAGFVGPVSAVIMGLIVGPLCYFAVSKM
                     KAKLGYDDSLDAFGIHGAGGIFGALATGVLANPAVNPAGTGLLYGNPHQLLVQLIAVV
                     ATIIFASAVTFLILKGLSRLAPLRVSDEEEENGLDLTQHGEDAYGYGEMSTIAV"
     gene            4860..5165
                     /locus_tag="P5549_08945"
     CDS             4860..5165
                     /locus_tag="P5549_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRW13237.1"
                     /translation="MARTSNIFARVEPEVKEQAELVLEQLGIPMSNAIGLFLRQVVLQ
                     RGIPFELKLPQSKPLSVSLLTEEQFNSEIEKGFADLTAGRVVSSEAVIDRMRQDYGI"
     gene            5162..5497
                     /locus_tag="P5549_08950"
     CDS             5162..5497
                     /locus_tag="P5549_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRW13238.1"
                     /translation="MSEWKVVYTEQAERDLRSIFEYIAFSLLEPEVAKNQSRRIMDMV
                     AKLNQMPLRYPLYAKEPLQSKGLRVLSVDNYLAFYLPVETKTTVAIIRIIYGGRDIEE
                     QLRQTEAEG"
     gene            complement(5695..>7612)
                     /gene="leuS"
                     /locus_tag="P5549_08955"
     CDS             complement(5695..>7612)
                     /gene="leuS"
                     /locus_tag="P5549_08955"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRW13239.1"
                     /translation="LSQWFFKITDYAQRLLDNLEVLPGWPEKVKTMQRNWIGRSEGCE
                     FDLAIEGREEKMTVFTTRPDTVFGVTYMVLSPEHPLVDIISAGTEQEAAVKEFVHRMQ
                     FISDVARTSTDTEKEGVFTGAYAINPMNNERIPIWIANYVLMDYGTGAIMAVPAHDER
                     DFDFARKYNIPIRIVIKSEDSPADDSQMTEAYTGSGKMVNSGEYDGLAYEEGKKKITA
                     YMESIGIGKATINFRLRDWLISRQRYWGAPIPIIYCDDCGALPVPEKDLPVLLPENVA
                     FKPSGESPLTYVQSFYETVCPCCGKPARRETDTMDTFVCSSWYFARYCDPHNSELPFA
                     KDKTDYWMAVDQYIGGVEHAILHLMYARFFTMFLHDIKMLSVEEPFTNLLTQGMVLKD
                     GSKMSKTKGNVVSPEEIIKTYGADTARLFILFAAPPERDLEWSDQGVEGSYRFLNRVW
                     RIVTECMEMIQDVNLPETFAELDPEARELRYRTHAAIKKVSEDVGERFNFNTAISAIM
                     ELSNSINSYRNQDELDLPVLKEAIEAMLLLLSPFSPHIAEELWQYCGHQTSIYSQSWP
                     EWDESALVKDEVEIALQVLGKVRDRVMVPSEISKEELEKIALANERVQEFSQSKQVVK
                     VIVVPGKLVNVVVR"
BASE COUNT         2002 a         1648 c         1904 g         2058 t
ORIGIN      
        1 ggaagccctg caagctgcca acaaactgga atatccggtt ttaatgcggc cttcttatgt
       61 gctgggcggg caaaacatga tcattgcttt cagcgatgat gatatccgcg aatacatgga
      121 cattattctt gcctacaaca ttgaaaatcc ggttttgatc gataaatatc tcatggggat
      181 cgaaatcgag atcgatgcca tctgtgacgg agaggatata ttaattcccg gtatcattga
      241 acatattgaa cgagccggca tccattccgg tgactcgatt gccgtatatc ctgcctggaa
      301 tctcagcgga gaattgatcg agcaggtgat cgatgattcg aaaaaactgg ccctggccct
      361 ggacacccaa ggactggtca atatccaata tgtcattcat ggcggagaga tctatgtgat
      421 tgaagttaac cccaggtctt cccgaacgat tccctatatc agcaaagtga ccggggtgcc
      481 gatggtggat ctggccaccc gggcaatgct gggtgagaaa ttacgtgata tgggctatgg
      541 tacgggctta tacaaaacgc cgccttatgt tgccgtcaag gtgccggtct tctcctttga
      601 aaaactgatg gatgtggatg tcgcgctggg accggaaatg aagtctacgg gtgaagtgct
      661 ggggatcggc aaaaccctgg cagaggctct gtacaaggga ctggtggcgg ccggttacaa
      721 gatggtaaaa caggggggtg tgctaattac cgttcgggac acggataagc ctgagatcgt
      781 tgatattgcc agacaatacg ctgaactggg tttcacgctg tatgccaccg gtggaacggc
      841 caatcttctc aatcaagccg gactgccggc gatttcggtc aagaagatcc atgaagccga
      901 ccatgacaat atccaaacgc ttttggaaag cggccggatc aattatatta tatccacttc
      961 ctccaagggc agactgcccg gtcgggacag tgttaaaatt cgtcgtaagg cggtggagtc
     1021 agccattccc tgcctgacct cgctggatac cgccagtgct ttggccaaca gcttgaagag
     1081 ccgctattcg caaagcaata ctgagttggt ggatatcaat catatgagga acgaacgtat
     1141 gcggctggct tttaccaaaa tgcagtcctg tggcaacgat tatatctatt ttgattgttt
     1201 tgaccaggaa atcgtaagtc cggaatcctt aagcgtatat ttatctgacc ggcattacag
     1261 cattggcagt gacgggatcg tactgatctg tccttccgaa atagctgatg cgaaaatgaa
     1321 gatgtttaac ctggacggca gcgaaagcgg aatgagcggc aatgccctta cctgtatggg
     1381 gaaatatatt tacgagaata acattgcagt caaagaccag attactatag aaacgctgag
     1441 cggcataaaa aaactgaaac tttatgttaa aaatgaaaaa gtgcattcag tctgcatcga
     1501 tatgggaccg gctgagctaa gacatgagaa aataccggtc tatctggacg gtcaacaaat
     1561 tattgatcgg ccggtcaata ttgccggtcg tgaataccgt attacctgtg tgtccatggg
     1621 caatccgcat tgcgtcgttt ttgacgagag tctggccagt attgatatgg atgaggtcgg
     1681 gccggctttt gaatattcga tgctatttcc tgaaagagtg aatgttgaat tcgtgacggt
     1741 cattgatcaa aatacgatca aaatgcgggt ttgggaacgc ggcaacggcg aaacgctttc
     1801 aaccgggacc agtgcctgcg ccggggttgt cgccgccgta ctgaatggtt ggtgtcgcaa
     1861 ggatcagcat attctggtca aacaaaaagg cggcaattta atcgtcaaat atacggatga
     1921 tacggtattc atgaccggca aaccggaaac tatttttgca ggaaccgttg aaatataatc
     1981 aaggatcagt ctgaaagtat acttcataca tccaaaaaac aggttgtatt tgcagcagaa
     2041 aagcatatta tatttatata ctacaagttc ataaatccag ccggtcaagg ctgaaccaag
     2101 acgatgaagt ctccgtgtaa atacggagac tttctgtttt tattggtctt caggcaaatt
     2161 tgaaacagga agttgaaata ttgagggcaa aggtgtccgc agaatagtcc ttacgcaagg
     2221 attattgtgc cggcaccttt tcattttaga aaccaattta gaatcaagca aattcaagtt
     2281 caaagggggt cgttttatga aaaaaataga ggcaatcatc agacctgcca agctggaaga
     2341 attgaagaca gccctggaag cggtaggtgt ccggggaatg acggtctacg atgtcaaggg
     2401 ccgcggttta cagtgcgctg agaaacaata ttaccggggg caggaacatt ccattgattt
     2461 gtttcccaaa gtaaagctgg aaattgtgtg tcaggatgag tcagttgaaa atatcatttc
     2521 gcaaataatc aaaacctgca agacaggcaa gaagggcgat ggaaagatat ttgtctatcc
     2581 ggtggaacgg atcatccgca tcagcacccg ggaagaaggg tgcgctgccc tgtaaaaagc
     2641 agggagagtt cgatactgat aagcaaaggc gcttatggta gaaaaagatt tctgaccata
     2701 ggtgccttta ctattgcgaa atatcctgca gcgataatat ttcagaatca aaaaagtcag
     2761 aaatgattaa ggaaagaagg ggtatacatg aaaacaaaat ccaaatggct ttcaatgtta
     2821 acaatactgg ctgcattcat attgggactg ccggctgtcg cattcgcagc cgatgcaccg
     2881 acgatgaaca gcggtgatac agcctttatg attatttgtg ctgctttggt gatgatcatg
     2941 acgccaggtc tggcattctt ttacggaggc atggtgcgca agaaaaatgt tatgggtacc
     3001 atcatgcaga gttttgctgc cctggccctg gtatcggtgc agtgggtgct gctgggttac
     3061 accttgtcct ttggcccgga tatgcatcat ttgctgggag gcttgagctt tctggggctg
     3121 tccggagtaa ccggtgatcc cagtgcgaat tatgcaaaca ccattcctca ttttgttttc
     3181 gtcttttatc agctcatgtt tgcgattatt accgtggctt tgatctcagg cgcctttgct
     3241 gaaagaatga gattttcgat tttcgttgct tttgtgttgc tctggacgac ccttgtctat
     3301 gatccgctgg cgcactgggt atgggctgcg gatggctggc tcttcaacct gggagcattg
     3361 gattttgccg gcggtactgt cgttcatatc agctccggta tctcaggact ggtggcctcc
     3421 attttactgg ggcgccggaa caagtcaggc gcagagccga tgatccccca caatttaccc
     3481 tttacgatgc tgggggctgc gctgctgtgg ttcggatggt tcggctttaa tgccggcagc
     3541 gccctggctg caaacggttt ggccgcttcc gccttcctgg tcaccaatac ctgcgcggcg
     3601 gccggtgcca tcggttgggc ggcggcagag tggctgcagc atggcaaacc caccacgttg
     3661 ggcgtagcca gcggactggt agcaggactg gtagctatta ctccggctgc aggttttgta
     3721 ggtccggtca gtgcagtgat catgggtttg atcgtaggcc cgctgtgcta cttcgccgta
     3781 agtaaaatga aggccaaact cggttacgac gattcactgg acgccttcgg gattcatggt
     3841 gcaggcggaa tcttcggagc gctggcgaca ggagtactgg ccaatcccgc cgtcaatccg
     3901 gccggcactg gattactgta cgggaatcct catcagttgc tggtgcagtt gattgccgtc
     3961 gttgcaacta taatattcgc ctcagcggta accttcctaa tcctgaaggg gttgagccgg
     4021 cttgctccat tgcgggtatc cgatgaagaa gaagaaaacg gtctggatct tacccagcat
     4081 ggtgaagatg cttacggtta tggagaaatg agtacgattg ccgtttgact gtgatgctga
     4141 aaaatcgtgt ctggattttt acgaaaatga aattttcttt tgaaattaca gtcgtaaatt
     4201 cgaaaaatga ttttgctttt tataaaatcc ggattgattt tatgaaattc ccgatgagtt
     4261 ttaatgaaaa accattttga ttattaaaac aagaattggt ttttcaattc aacgcttgtt
     4321 tgttaaaatt tgtcggcgac ttggagctac cttaagcatg gaattttaca ttttttcaaa
     4381 acacaaatcg cgttagtcag gcattcttga tgcagaaatg tttcacgtga aacattatgt
     4441 tcagttgtgg aataaaacgg atcagttcca gacaaacttc gcgaaaaaat cgtactactt
     4501 ttaatgggat agagaaacat tcgcattcag tagaggagag atgtcatttg aattccgttt
     4561 gcgaatcgtt tatcaatatt ttcgcccatt tatcaatcgc ccttctcttg tgtattttaa
     4621 aaaagaatgg cactacagag atgaacaagg tctgtcattc tccaatagtt ttgcagattt
     4681 tatgaacacg gagacggttt atatttcaaa ttccttaccg tcttccttca gttccgtctt
     4741 atcggaaatt tatgactaat caagttatcg tattaaaatt agatctagga tcgttagcta
     4801 acagattgca ttccgcaata caatttagta taataatggc agaataggag gtcttcgtta
     4861 tggcaagaac atcaaatata tttgcccgtg tcgagccgga agttaaagaa caggcggagc
     4921 ttgttttaga gcagcttggt atcccgatgt caaacgcaat cggacttttc ctcagacagg
     4981 ttgttcttca gcgagggatt cctttcgaat tgaagctgcc gcaaagcaag ccactgtcag
     5041 taagtttgct taccgaggaa caatttaatt ccgaaatcga gaaggggttt gctgatttga
     5101 ccgccgggag agttgtttct tccgaagccg tcatcgatag aatgcgtcag gactatggca
     5161 tatgagcgaa tggaaggttg tctacacaga gcaagcagag cgtgacttac gcagcatatt
     5221 cgagtatatt gccttttcct tgcttgagcc agaggttgca aagaatcaat cacggcgaat
     5281 tatggacatg gtcgcgaagc tgaatcaaat gccgctgcga tatccccttt acgcaaaaga
     5341 gcctttgcaa agcaaaggct tgagggtttt gtcggttgac aactatttgg cgttttatct
     5401 gcctgtagaa acaaaaacga cagtggcaat tatccgaatt atatatggcg gacgtgatat
     5461 tgaggaacaa ctacggcaaa ccgaagcaga agggtaaaca ggattaaaac agcgttcagt
     5521 taaaaatact tatacggcat gaagttgatt gttttttaaa cgacgttttt aattcttcaa
     5581 aagtccagcg gatactggtt aaaaagaaga aatgagataa aaacacaaag gccttctgat
     5641 ctattacgaa tcagaaggcc ttgacaattt aaaaagttga tacgccggtg cggcttatct
     5701 cactaccaca ttaaccagtt tccccggtac cacaattact tttacgacct gcttactctg
     5761 gctgaattcc tggacccgct cgttggccag ggcgattttc tccagctctt ctttgctgat
     5821 ttcgctgggg accatgactc tgtcacgaac cttgcccaga acctgcaatg cgatttccac
     5881 ttcatccttg accagagcgc tttcatccca ttcgggccat gactgactgt aaatgctggt
     5941 ctggtgaccg caatattgcc acagttcttc tgctatgtgc ggggagaacg gacttaagag
     6001 cagcagcatc gcttcaattg cttctttgag gaccgggaga tccaattcgt cctgattgcg
     6061 atagctgttt atactgttgc ttagttccat gatggcactg atggcggtat tgaagttgaa
     6121 acgttctcca acatcttcgg acactttttt tatggctgca tgggtgcggt atcttaactc
     6181 ccgcgcctcg ggatcaagtt cggcaaaggt ttccggcaga ttcacatcct gaatcatctc
     6241 catgcattcg gttacgatgc gccagacgcg gttgaggaaa cggtaagatc cttccacgcc
     6301 ctggtcgctc cattccagat ctctttccgg aggtgcagcg aagagaatga aaagacgtgc
     6361 cgtatcagca ccgtaggttt tgatgatttc ttcgggactt actacattgc ctttggtttt
     6421 tgacatttta ctgccgtctt tcagaaccat gccctgggtc aacaggttgg taaagggctc
     6481 ctcaacggaa agcattttaa tgtcatgtaa gaacatggtg aagaagcgtg catacatcaa
     6541 gtgcagtatg gcatgttcaa cgccgccgat atattgatcg actgccatcc agtaatcggt
     6601 tttgtctttg gcaaatggca gttcagaatt gtgcggatca cagtaacggg caaaatacca
     6661 cgatgaacaa acaaacgtat ccatggtatc ggtttcccgg cgggcgggct tgccgcagca
     6721 gggacatacg gtttcataaa aactctgcac ataggtcagc ggtgattcgc cgctgggctt
     6781 gaaagctacg ttttcaggca gcaagaccgg cagatctttt tcaggtaccg gcaatgcacc
     6841 gcaatcatca caataaatta tggggatagg tgcaccccaa tagcgctggc gggagatcag
     6901 ccagtcgcgc agacggaagt tgatggtagc tttaccgatt ccaatggatt ccatatatgc
     6961 ggtgattttt ttcttgcctt cttcataggc aagtccgtca tattcaccgg aattgaccat
     7021 tttgcccgag ccggtatagg cttcagtcat ttggctgtca tcagccggtg aatcttcgct
     7081 tttgattacg atccggatgg ggatattgta tttacgggca aaatcaaagt cacgttcatc
     7141 atgcgccgga acagccatga tggcgccggt gccgtagtcc attaatacat aattggcaat
     7201 ccaaatggga attctttcat tgttcatggg gttgatggca taagccccgg taaagacccc
     7261 ttctttttct gtatctgtag aggtgcgggc cacatcgctg ataaactgca tgcggtgcac
     7321 aaattctttg actgctgcct cctgttcagt tccggcagaa ataatatcta ccagcggatg
     7381 ttcgggggac agaaccatat atgttacacc aaagaccgta tccggacggg tggtgaaaac
     7441 cgtcattttt tcttcccggc cttcgatggc cagatcgaat tcacagcctt cactgcggcc
     7501 gatccagttg cgctgcattg ttttaacctt ttccggccat cccggcagca cttccaaatt
     7561 atcaagcagt ctttgcgcat aatcggttat tttgaaaaac cattggctga gc
//
LOCUS       DAOTHZ010000088         7595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11597_length_7595_cov_5.936472, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000088 DAOTHZ010000000
VERSION     DAOTHZ010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11597_length_7595_cov_5.936472"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..939)
                     /gene="dpsA"
                     /locus_tag="P5549_08960"
     CDS             complement(25..939)
                     /gene="dpsA"
                     /locus_tag="P5549_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HRW13240.1"
                     /translation="MSSVLSGIKIAVVGGDDRELILVNELVRMGATVTVCGFPREIVG
                     HGAFVLNNPEEACKGAEVVVLPLPGTSSEGVIRAVYAEQELKLTEKTIAALAPHALVM
                     IGTAREFLKKWVGERKLNLLEVAEIDEIAVLNSIPTAEGAIQIAMEETDFTIHGSNSC
                     VIGFGRIGITLARNLKALGSEVTVVARNRGQLARAYEMGCNQAELNDLKEIMHSADLA
                     FNTIPHLVLDRTVLKYANPHAVIIDLAAQPGGTDFEAANHYGLKAILAPGLPGKVAPL
                     SAGKILANVIPGLIIKELVKQEQVSMFA"
     gene            complement(1140..1940)
                     /gene="dapB"
                     /locus_tag="P5549_08965"
     CDS             complement(1140..1940)
                     /gene="dapB"
                     /locus_tag="P5549_08965"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRW13241.1"
                     /translation="MAEKIKVIVSGALGKMGKETVKAVFYEPALDLVGIVDVRGKGES
                     MGDILAIKELVLPVESDLEQLISSVQPDVMVDFTNPQAVFNNTRTALKHKVNCVIGTT
                     GLNELELAQLEKIALENQVGVAIIPNFALGAILMMKLSQQVAQYFPDVEIIELHHDQK
                     LDAPSGTAIKTAEMITANRQRIPARNAREFEKVTGARGGEVNGIRIHSVRLPGFVAHQ
                     EVIFGGIGQSLSIRHDSYDRVGFMPGVLMTVKKMTEMKGLVYGLENFL"
     gene            2119..3903
                     /locus_tag="P5549_08970"
     CDS             2119..3903
                     /locus_tag="P5549_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRW13242.1"
                     /translation="MPFDGFTIRCLCRELDQALNNARIDKIHQPERDELNLTLRQMKS
                     SNQRLLLSANARWARFHLSTDKKPNPSQPSAFCMLLRKYLEGGKIKEIKQIGMERIIH
                     VRIEALDDFKEWKDKLLICEFMGRHSNIILVNPENNLILDAIKKYGSDVSSYREVLPG
                     KEYIAPPSQDKLDPAATVWEDFTAAMWNQPEDTDLAAAVFQVYAGISPYSARQICRQC
                     NLDPTAAVGQAGEFELQKIYYYIRDLLRAIDEGQTTPKVHYNHKKQPLEFGLFDGASE
                     AGSSFCRDYPSMNETCDAFYGHKMSMLRLESMKTNLLRNLKERLDKYYKKRFLQEGDL
                     SKALDNVIYKTYGELLTAYSHLFKKGDTQALVEDFDSGDAVALPLDPRYTPIQNAQRF
                     FKIYNKSRGAQKHLEVLMAQNQQEIDYLESVMVAVQQAENPAQIDEIMEELEKEGYMK
                     ANSGKGKKREERSQPRRFESSDGLLIMVGRNNRQNDWLTLREAEHHDLWLHTKEVPGT
                     HVIIKLPAHFKSIHEVPDQTLIEAATLAAYFSKASQSNKVAVDYTFSQNVKKPNAAKP
                     GMVIYENYWTIMANPCSALAEHLLEKGS"
     assembly_gap    3917..4016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4351..4689)
                     /locus_tag="P5549_08975"
     CDS             complement(4351..4689)
                     /locus_tag="P5549_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13243.1"
                     /translation="MKRRSAAMMKEGIKTKVFLNGTEMQFVEGGYQYVFVKPYQKYTE
                     KTVNKENGDKMHLELYDNGVQIRTLVTQQEVSTIINRDVAVDTVNNKIYILEPDSQVR
                     KNEDGSVELI"
     gene            complement(4695..5267)
                     /gene="pyrE"
                     /locus_tag="P5549_08980"
     CDS             complement(4695..5267)
                     /gene="pyrE"
                     /locus_tag="P5549_08980"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRW13244.1"
                     /translation="MLSKEQAIKIFSESGALLEGHFKLTSGRHSNRYMQCAQVLQYPA
                     YTEELARHLADRFADTKIDLVIGPAMGGIIVAYEVARQLQVPGIFTERQEGKMALRRG
                     FAIEPGARVLVVEDVVTTGGSVVEVMEVVRQLQGNVVGVGVLVDRSAGKVDFGVPLHS
                     VLAMDIESWEADQCPLCKQGQGEAIKPGSR"
     gene            complement(5257..5991)
                     /gene="pyrF"
                     /locus_tag="P5549_08985"
     CDS             complement(5257..5991)
                     /gene="pyrF"
                     /locus_tag="P5549_08985"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRW13245.1"
                     /translation="MDPKERIILALDVDTHEEALQLVKTLTPYVGAFKIGMQLYNSVG
                     PSIVEEVNQCGGKVFVDLKFHDIPNTVASAGKVLTRQHCYMFNVHAAGGREMMQRTAA
                     EVKAEAASLGIEAPLILAVTVLTSITDQQLKEEMYVEGINVRELAVKWAALAKASGLD
                     GVVCSPKEITAIRDACGPDFKIVTPGIRPLWSEVNDQKRITTPWDALDLGADYMVIGR
                     PITKAENPRAAATRIIEELEGNSHAE"
     gene            complement(6162..6602)
                     /locus_tag="P5549_08990"
     CDS             complement(6162..6602)
                     /locus_tag="P5549_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13246.1"
                     /translation="MRVAIAAPHGRSGKTTVTLGLIAALRRAGLTVQPFKNGPDFMGS
                     REEDLYLDTYNNTTLKEALKRYEIRQVVDLIAAWTDQELLNLVGIIERLNTYRSPLDA
                     QYNEIKTFLERSTNEERQVILDSIELIKKPELKDGLLERIEEGK"
     gene            complement(6810..>7595)
                     /locus_tag="P5549_08995"
     CDS             complement(6810..>7595)
                     /locus_tag="P5549_08995"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRW13247.1"
                     /translation="EPVAAAVVPYGTVRASFVKGDITVADAFNVSSLGIGKDKQSGYP
                     LISVYLTGQELKTACEVDASITPLMKPAQLYLAGMTYTFNPHRLLFNKVTDAALLRAD
                     GTRLEIEDNKLYRVVAGLYSAQMLSVVGDKSFGILSIVPKTREGQPITDFEAQIITDA
                     EGREIKEWVALARYLQSFAINQSGLPQVPAFYQQAQGRKIVNQDTSLQAILSQPNRIA
                     LIFYGSVLLLICLLILLIHLIVQRIQRRRISQTPEVLRLPSRE"
BASE COUNT         1841 a         1984 c         1733 g         1937 t
ORIGIN      
        1 atgttgcact tttccacctc cttgctaggc aaacattgaa acttgttctt gtttcacgag
       61 ttccttgata atcaaccctg ggattacatt ggccaggatc tttccggctg acaaaggtgc
      121 taccttgcca ggcaaaccgg gtgcaagaat agccttcaga ccgtaatgat tggccgcttc
      181 gaagtcagtt cctcctggct gcgctgccag atcaataatc actgcatggg gattggcata
      241 ttttaaaacc gtccgatcca agaccaagtg cggaatggtg ttaaaagcca gatctgcgct
      301 gtgcatgatt tccttcaaat cgttcagttc tgcctggttg catcccattt cataagcccg
      361 ggccagttgg cctcgattcc tggcgacaac ggttacttcc gatcccagtg ccttcaaatt
      421 gcgagccagg gtaatcccta ttctgccaaa cccaatcaca cagctattac tgccatgaat
      481 ggtaaaatcg gtttcttcca tggctatttg gattgcccct tccgctgtag gaatcgagtt
      541 taagacagcg atttcgtcta tctctgctac ttccagtaaa ttcagtttcc tttcccccac
      601 ccatttcttt aaaaattcac gggctgtgcc aatcatcacc agtgcgtggg gtgccaaagc
      661 ggcaatggtt ttttcggtca atttcaactc ctgttcagca taaaccgccc gaataacacc
      721 ttctgaactg gtccccggca aaggtaatac gaccacttca gcccctttac aggcttcttc
      781 cggattattc agcacgaaag ctccatgacc gactatttcc cgcggaaaac cacatactgt
      841 tacggtagcg cccatgcgta ccagttcatt aaccaggatc aattcacgat catccccgcc
      901 tacgacagca attttaatac cggacagaac tgaactcacc ctattccctc ctcaggacat
      961 ttactaattt gtgcggaatt attagcaata tatgaggagg gcttataagt ggtgcttgtc
     1021 ctgccggggg atggggtgcg atttttgtat ttacggcaat aaattgcaca aaaaaaccgt
     1081 cccgcccggt cagggattga accggggaga cggattgagt gaatgcgatc cggaatggtt
     1141 tacaggaagt tttccagtcc gtagaccagg cctttcatct ccgtcatttt tttcacggtc
     1201 atcaagacgc ccggcataaa accgactcga tcgtaggaat catggcggat gcttaaactc
     1261 tgcccgattc ccccaaaaat aacttcctgg tgggcaacaa atccaggcag acggacactg
     1321 tggattctga tgccgttgac ctcaccgcca cgcgctccgg tcactttttc aaattcccgg
     1381 gcattgcggg caggaatacg ctgccggttg gccgtaatca tctcggcggt tttaatggct
     1441 gtgccggaag gagcatccag tttttgatca tggtgcagtt caatgatttc aacatcgggg
     1501 aaatattgtg ctacctgttg ggataatttc atcatcaata tcgcacccaa ggcaaaattg
     1561 gggatgatcg ccaccccaac ctgattttcc aatgcgattt tttccaactg agccagttcc
     1621 agttcgttca aaccagtcgt cccaatgaca caattcacct tatgtttcag cgccgtccgg
     1681 gtattgttga aaacagcctg gggattggtg aaatccacca ttacatctgg ttggacggag
     1741 ctaatcagtt gttccagatc gctttccacc ggtaatacca attccttgat cgccagtata
     1801 tctcccattg actcgccttt gccgcgtacg tcaacgatgc caaccagatc gagcgcaggt
     1861 tcataaaata ctgccttgac ggtttccttg cccatcttgc ccagcgcccc ggacacgatg
     1921 accttgatct tctctgccac gacgtaacct ccctgttgtt tataatgaat agttttcact
     1981 atttcgctgc tgtcgtcaag attgccggac acgaatccgt ccaattccag cgctgattga
     2041 agctgtagca cgggaagtgg tatactgagt gctgatgaag cagtgaagac ctgattatct
     2101 gcaaaggaga acaaaacggt gccttttgat ggatttacaa tcagatgcct gtgccgggaa
     2161 ctggaccagg cattaaacaa tgcccgcatc gacaagattc accagccgga acgggacgaa
     2221 ttaaatctta ccctgcgaca gatgaaaagc agcaaccagc ggctattatt gtctgccaat
     2281 gcgcgttggg ccagatttca tttaagtacc gataaaaaac ccaaccccag tcagccttcg
     2341 gctttttgca tgttgctgcg caaatatttg gaaggcggca aaatcaagga aatcaaacaa
     2401 atcggcatgg aacgcatcat ccatgtgcgg attgaagcgc tggatgattt taaagaatgg
     2461 aaggacaagc tgctgatttg tgaatttatg gggcgtcatt ccaatatcat cctggtcaat
     2521 ccggaaaata atttgatttt ggatgccatc aaaaaatatg gcagtgacgt cagttcctac
     2581 cgggaagttt tgcccggcaa ggagtatatt gctccgcctt cgcaggataa actcgaccct
     2641 gccgcgacgg tctgggaaga tttcaccgcc gccatgtgga accaaccgga agataccgat
     2701 ttggctgcgg ccgtttttca ggtttacgcc ggcatcagtc cgtattcagc ccgtcagatt
     2761 tgccggcaat gtaatcttga cccaactgca gcggtcgggc aagcgggtga gttcgaactg
     2821 caaaaaatat actattacat ccgcgatctg ctgcgggcca ttgatgaggg acaaacaact
     2881 ccgaaggtgc attataatca caagaaacag ccgcttgaat tcggtttgtt tgacggcgcg
     2941 agtgaggctg gcagctcttt ttgccgggat tatcccagta tgaatgaaac ctgcgatgct
     3001 ttttacgggc ataaaatgag catgctgcgc ctcgaatcga tgaaaaccaa tctgctgcgc
     3061 aacctcaaag aacgtctcga caaatattat aaaaagcgtt tcctgcagga aggcgacctt
     3121 tccaaggctt tggacaatgt tatctacaaa acctacggtg aactgctgac cgcctattcc
     3181 catttgttta aaaaaggaga tacgcaggcg ttggtcgagg acttcgacag cggtgatgca
     3241 gtcgccctac ccttggaccc tcgctatact ccgattcaaa atgcccagcg cttttttaaa
     3301 atctataata aaagccgcgg cgcacaaaaa catttggaag ttttgatggc ccagaaccag
     3361 caggaaattg attatcttga atccgttatg gtggccgtgc aacaagcgga aaatccagcc
     3421 cagatcgatg aaatcatgga agagctggag aaggaaggct atatgaaagc caattccggc
     3481 aaaggcaaaa agcgggaaga aagaagccag ccgcgacgtt tcgaatcctc tgacggactg
     3541 ctcataatgg taggccggaa caaccgccaa aatgactggc tgacgctgcg ggaggcagag
     3601 catcatgatt tatggctgca taccaaggaa gtacccggca cgcacgtcat tatcaagctg
     3661 cctgcacatt ttaaatccat ccatgaagtc ccagaccaga cgctgattga ggcggctacc
     3721 ctggcagcct atttcagcaa agccagtcaa tccaacaaag tcgcggttga ttataccttc
     3781 agccagaatg tgaaaaagcc caatgccgcc aagccgggca tggtcattta tgaaaattac
     3841 tggacgatta tggctaatcc ctgctccgcg ctggcagaac atttactgga aaaaggcagt
     3901 tgaacaacat tgctgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattg
     4021 ctgtgatgag tcagaaagcc aaacggcctt tcctgcagga aaggccgttt ggcttgaaca
     4081 taaaccctta tataggtcac agtgtaaata tgatgggaga aatcatattc gccaatttat
     4141 aatgcaacat tcgtgccagc ttatccgagc cgctgccatt ctgcccgtcg aacggcaaat
     4201 ggccctaatc gacgcccttc cttccgatta gtgcacaaat gatttcccca taccccttgc
     4261 gcagtatggt tacacaagtg cctcaaacct gtcacctttt tgttcgtttt cgtgtcacgc
     4321 tgcccaatta taatgcgcaa actcaggcgt ttatatcaat tccacggagc catcttcatt
     4381 ttttcgcacc tggctgtcgg gttccaggat atatatttta ttgttaacgg tatccaccgc
     4441 tacatcccgg ttgatgatgg tactgacctc ctgttgggtc accagggtgc ggatctgtac
     4501 cccgttgtcg tacaattcca gatgcatttt atcgccattt tccttgttga cggtcttttc
     4561 ggtatatttc tgataaggtt tgacaaaaac gtattggtac ccgccttcga caaactgcat
     4621 ctccgttccg tttaaaaata ctttcgtttt gatcccctcc ttcatcatcg cagcggatcg
     4681 tctcttcaac cgctttatct gctgccgggt ttgatagcct cgccttgtcc ctgcttgcac
     4741 aaaggacact ggtctgcctc ccaggactcg atatccatcg ccagcaccga atgcagcggc
     4801 acaccaaaat caaccttgcc ggcactgcga tcaaccaata cgcctacgcc gacgacattc
     4861 ccttgcaatt gccggacgac ttccatgact tccaccaccg atccgccggt ggtaacaaca
     4921 tcttccacca ccagcacccg tgccccgggc tcaatcgcaa atccacgccg caacgccatt
     4981 ttaccttcct gccgttcggt aaagatacca ggcacctgca actgccgggc aacttcatag
     5041 gcaacgatga tgccgcccat ggccgggcca atcaccaggt caatcttggt atcggcaaac
     5101 cgatcggcca gatgccgggc cagttcttct gtataggcag ggtattgcag tacctgggca
     5161 cactgcatgt aacgattgct gtgccgtccg gaagtcagtt taaaatgccc ttccagcaaa
     5221 gcgcccgatt cagaaaagat tttgatcgct tgttccttac tcagcatggc tgtttccctc
     5281 caactcttcg ataattcttg ttgccgctgc ccgcgggttt tccgctttgg taattggccg
     5341 gccgatgacc atgtaatcgg cccccaaatc cagagcgtcc cagggtgtgg taatacgttt
     5401 ttgatcattg acttccgacc agagcggtct gatgccgggt gttactattt tgaaatccgg
     5461 tccgcaggca tcccgtatgg ccgtgatttc cttgggcgaa cagacaactc cgtcgagtcc
     5521 tgaggctttt gccagggctg cccatttgac tgccaactcg cgaacattta taccttccac
     5581 atacatttcc tctttgagct gttgatctgt aatactggtc agcacggtca ctgccagaat
     5641 gagcggcgcc tcgattccca aagatgctgc ctccgcttta acctcagcgg cggttctttg
     5701 catcatttcc cgcccaccgg cggcatgcac attaaacatg tagcaatgct gccgcgtcag
     5761 tactttcccg gcggaagcaa cggtattggg aatatcatga aacttgagat ccacaaatac
     5821 cttgcctccg cactggttga cttcctcgac aatggacgga ccgacgctat tatataattg
     5881 catgccgatt ttaaacgccc ccacataagg ggtcagggtt ttcaccaact gcagcgcttc
     5941 ctcatgggta tccacatcca gggccaggat aattctctct ttcgggtcca taaactgcct
     6001 ccttgcattc ataaatctgc tcgtataatt aatagtatta tggaaatcgg gcaacgagtc
     6061 aatgtttgat gattctctcc gttgcaatca aacaatttgg tcaaaaagaa aagggtttta
     6121 cctctctgct gaagaggtaa aaccctgcct ggttcaaata tttatttgcc ttcttcaatt
     6181 ctttccaaga ggccgtcttt taactctggc tttttaatca attcaatcga atccaaaatc
     6241 acctgacgtt cttcgttggt ggatctttcc aaaaaggttt tgatttcgtt atattgcgca
     6301 tccaatggac tgcggtacgt attgagacgc tcgatgatgc ccaccagatt gagcaattcc
     6361 tgatcggtcc aggcggcaat cagatcgacg acctgccgga tctcatatct tttcaaggct
     6421 tcttttaaag ttgtgttgtt gtaggtatcc agatataaat cttcttccct cgatcccata
     6481 aaatcaggcc cgttcttgaa gggctggacg gtcaatcctg ctcttcgcaa agcggcaatc
     6541 aaacccaagg ttacggttgt tttcccggaa cgaccatgtg gtgctgcgat ggctactctc
     6601 atttttctca ccctttgctg ccctcttgtc tgccgttccc ctgccggttt tccggccagg
     6661 cagttcccgc agtcttggtg ggcttgctgt aagcgcaata taacacagaa atatttgatc
     6721 gtataattta aaatatctat gctgccatta ggcctgacaa tggcaagagc agcttccgcg
     6781 gccggtatga attgatcatc cgtcctgcct tattcccggg aaggcagacg cagcacttcc
     6841 ggggtctggc tgatccgccg gcgctgaatg cgttgcacga tcaagtgtat gagcagaatc
     6901 agcaggcaga tcaacagtaa aacgcttcca taaaaaataa gtgcaatgcg gttgggttgt
     6961 gacagaatgg cctgcagcga agtatcttga ttgacgatct tgcgtccctg tgcctgctga
     7021 tagaaagccg gtacctgcgg cagccccgac tggttgattg caaaagattg caaatagcgg
     7081 gccagggcca cccattcctt gatttcccgc ccctcggcgt cggtgatgat ctgggcttca
     7141 aaatcagtga tgggttggcc ttcgcgggtc ttgggcacga tcgacaggat tccgaatgat
     7201 ttgtctccta ccacagacag catctgggcg gaatacaagc ctgccaccac ccggtagagc
     7261 ttgttgtcct cgatctcaag ccgggtgccg tccgctcgca gcagcgccgc atccgtcact
     7321 ttgttgaaca gcaagcgatg cggattgaag gtataggtca tgccggccag ataaagctgg
     7381 gctggtttca tgaggggcgt aatggatgca tcgacttcac aggcggtctt gagttcctgg
     7441 ccggttagat aaaccgatat caacgggtag ccggattgct tgtccttgcc gatccccagt
     7501 gaactgacat tgaaggcatc ggccaccgta atatcgcctt tgacaaaaga agcccgaacc
     7561 gttccgtagg gaactacggc tgccgcaacg ggttc
//
LOCUS       DAOTHZ010000089         7584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11624_length_7584_cov_6.772745, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000089 DAOTHZ010000000
VERSION     DAOTHZ010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11624_length_7584_cov_6.772745"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="P5549_09000"
     CDS             <1..718
                     /locus_tag="P5549_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13248.1"
                     /translation="MCDGACVQLGIGSMPNTVGALIAGSDLKDLGVHTEMLCDSFVDM
                     YNAGKITGARKTLDKGKMVYTFAMGTNKLYEFLDHNPVCASYPVHYCNDPHIIAQNDN
                     VMAINNCLEVDIYGQVASEASAGRQISGTGGQLDYIIGAFHSKGGKPIIALNSTVKKS
                     DGSLASRITPGLSPGTIVTVPRSITSYVITEYGGVNLKGKSTWERAEALISIAHPEFR
                     DELVKAAEHAKIWLRSNQIA"
     gene            768..1970
                     /locus_tag="P5549_09005"
     CDS             768..1970
                     /locus_tag="P5549_09005"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRW13249.1"
                     /translation="MNEVVIVSACRTAIGKFLGVYKDVPAKELAVTVGKEAIKRAGVP
                     ADTIDEIVMGQVYQHMQKSLPARIVGLECGLSHESNACVVNQNCTSAMRALEIACRNI
                     MVGATESALVVGVENMTQAPYMVPSARTGARMGDVKMIDALTHDALVDSLVPGHMAMT
                     AENVAAMYNITREQCDELAVMSHTRAINAIDSGVFKREIVPVEVTTKKGVTVIDTDEH
                     PIRGASMESVGKLKPAFKKDGGVVTAANASGINDGASAVVIMSKKKAEELGIKPLMKL
                     VNICAMGVDPQIMGIGPAEAIPKCLKQAGWKFEDVDYWEINEAFAPQFLGCGIRLKEK
                     YNMTLDLNKVNHNGSGIALGHPIGSTGLRIIVSLYYEMERLGLTKGGASLCVGTGPAM
                     ASLWTRDI"
     gene            2143..3300
                     /locus_tag="P5549_09010"
     CDS             2143..3300
                     /locus_tag="P5549_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13250.1"
                     /translation="MDFRLTEEQEMIRQMAADFAANEIAPYAIEWDKADSVPFDTVAK
                     MQDLGLMTIGIPAEYGGDGLDWLSKYLVIDEIGQGDAGLGTMVVASSLLASDPIWVGA
                     NDEQKKWWYGRMLEGAVCGFCLTEPGAGSDALGMSCKCVKDGNDYILNGTKQFITNGG
                     FAKNFSVFATLDKKMGNKGLCALMVDRDFPGVSIGPKEDKLGIRSTSTTQVIFEDVRV
                     PGWMMLGGEGKGTPILMETLDLSRTAIAALATGISTAALKAALAYANERQQFGKPIST
                     FQAISFMLADMATAVETSRLLYQKAAWLQDNDLPFKMPSNMAKMYAADSAMKVTVDAV
                     QVFGGYGYTKEYPVEKYMRDAKIMQIFEGTVQVQRMVIGKYLSQNGYNPNV"
     gene            complement(3552..4430)
                     /locus_tag="P5549_09015"
     CDS             complement(3552..4430)
                     /locus_tag="P5549_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW13251.1"
                     /translation="MKAVLGEGIEILDRFKTEVPTIPQMFYHTVQHHGSKPANTYKAC
                     QEWKTVSYAEWYGIAEEIAVALIRRGVRKGDDIAVMSQVSAQRGWINMGILMCGAVVN
                     TITPLVDDDELKFIINRSDIKYLFVENLAMLNRAISLRSQMPTLKGIICLDEKYSGGR
                     NHLWGLQDFRLMGRNRSLRPVDLRQYWQRLTADDSAVLDYRKSATGQLKCTPMKHGDW
                     IRMERSRDRVLLSANLQGRYNNVLACVLPRSTSQEWVSGFCSRVAVGALIKYGAGPSK
                     INRLQELKEIRPAIAG"
     gene            complement(4713..5513)
                     /gene="spo0A"
                     /locus_tag="P5549_09020"
     CDS             complement(4713..5513)
                     /gene="spo0A"
                     /locus_tag="P5549_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRW13252.1"
                     /translation="MFAEGNQIKVLVADDNREFCGILRDYFSNDPDFELIDVCANGME
                     VLDVIAKKEVEVLILDLIMPYVDGIGVLEKLNEMNLNPRPKVVILTAFGQENITQKAV
                     QLGADYYILKPFNLQVLGERIKQVARDVKMKTPKGSSMTISMPSSVPKDLEVEVTKII
                     HEIGVPAHVKGYQYLRDAIMLVVDEINYLGAVTKELYPTIAQKYDTTPSRVERAIRHA
                     IELAWDRGDYDKINKFFGYTISGEKGKPTNSEFIAIIADKLRLENKVS"
     gene            complement(5683..7065)
                     /gene="spoIVB"
                     /locus_tag="P5549_09025"
     CDS             complement(5683..7065)
                     /gene="spoIVB"
                     /locus_tag="P5549_09025"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HRW13253.1"
                     /translation="MYSYRKLAGIVLAVLLLCLCCSPQARTLFSLPEKQKIVVGQANA
                     IRLSLPKTLQNQIDFTVLEHTRRVFLLQSYPAVTVQKKGSVYEIKALQPGRAELSMKL
                     LGYIPLKSIAVEALPNKRVVVGGHSIGVMLQSRGIMVVGFAPISQTDGSKVYPGKQQG
                     IEIGDLIYQVDDRPITNEEDLAKVIDSKKNQSLEVKLQRRGKTVSVPVRAEFCSETNR
                     YRIGLYVRDGVVGVGTLTFIDPGNGQFAALGHVIMDADTKQGIEVLSGKLLTASIQMI
                     RAGKPGHPGEKIGVFDEAGAFTGKILKNTDAGIYGIINNSKNFQPTTPQLEVAYAHQV
                     QVGAAQMWTVVNGNTIEKWDIQIEKVFPQRENGKGMIIKVTDPRLLSISGGIVQGMSG
                     SPIIQDNRIVGAVTHVFLNDPTSGYGIFMDSMLSEMPAMVSSQKTFRQFAGRVEAVES
                     SSHGDSLKPL"
     gene            complement(7213..>7584)
                     /locus_tag="P5549_09030"
     CDS             complement(7213..>7584)
                     /locus_tag="P5549_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRW13254.1"
                     /translation="GEISRLIFALKTSLAEKYQFPTLVFDEIDAGLGGTALTAVAQKA
                     SRLAARHQLILVTHSPQLASYADQHLHIDKYVENGRTFTRVAQLDEPERIRELARMLD
                     GDDYTELTLEHARELMNHRSK"
BASE COUNT         1949 a         1954 c         1747 g         1934 t
ORIGIN      
        1 gatgtgtgac ggtgcctgtg tgcagcttgg gattggttcc atgcccaaca ccgtgggggc
       61 cctaatcgct ggatctgatt tgaaggattt gggcgtccat accgaaatgc tgtgtgactc
      121 cttcgtagac atgtacaatg ccggcaaaat caccggagca agaaaaaccc tggataaagg
      181 taaaatggtc tatacctttg ccatggggac caacaagctc tatgaattct tggatcacaa
      241 cccggtatgt gccagttatc cggtgcatta ctgcaatgat ccccacatca tcgcccaaaa
      301 tgacaatgta atggccatca ataattgtct ggaggttgac atttatggac aggtcgcctc
      361 cgaagcttcg gccgggcgcc agatttccgg caccggcgga cagttggact acattatcgg
      421 tgctttccat tccaaaggcg gcaagccaat tattgccctg aactcaacgg tgaaaaaatc
      481 ggatgggagc ctggcttcca ggatcacccc cggtttaagc ccaggcacga ttgtaaccgt
      541 accccgctcc attacttctt acgtgattac agagtatgga ggcgtcaatc tgaaaggcaa
      601 aagcacctgg gagagagcag aagcattgat cagtattgct catcctgaat tccgtgatga
      661 actcgtcaag gccgctgaac atgccaagat ttggctaaga agcaatcaaa tcgcctaagt
      721 gtaaaagtga aaagtgcagc attataaaat gaaaggggaa ctgtaaaatg aacgaagtcg
      781 taatcgtaag tgcatgcaga accgcgatcg gcaaattcct gggggtctat aaagatgttc
      841 ctgccaaaga actggccgta accgtcggca aagaagccat caaacgggcc ggtgtccccg
      901 ctgacaccat tgatgaaatt gtgatgggac aggtctatca gcacatgcaa aagtctcttc
      961 cggcccgtat tgtcggactg gaatgcggtc tgtcacacga aagcaatgcc tgtgtggtaa
     1021 accagaattg cacctcggcc atgcgagccc tggaaatcgc ctgccgcaac attatggtgg
     1081 gagcaaccga gagtgccttg gtcgtcggcg tggaaaacat gacccaggcg ccttacatgg
     1141 tacccagtgc cagaaccggc gcgcgcatgg gtgatgtcaa aatgattgat gccctaaccc
     1201 atgatgccct ggtggactct ctggttcccg gccacatggc catgactgct gaaaatgtag
     1261 cagcgatgta taatatcacc cgcgaacagt gtgacgaatt ggctgttatg agtcataccc
     1321 gggcgatcaa tgcgattgac agtggtgtgt tcaaaagaga aattgttccg gtggaagtaa
     1381 ctaccaaaaa aggggttacg gttatcgata ctgatgaaca tcccattcgc ggtgccagca
     1441 tggaaagcgt tggtaaactg aaaccagcct tcaaaaaaga tggtggtgtg gtcaccgcag
     1501 ccaatgcttc cggtatcaat gatggagctt ctgctgttgt cattatgtcc aagaaaaaag
     1561 ccgaagaact cggcatcaaa cccctgatga agttggtcaa tatttgcgcc atgggcgtcg
     1621 atccccagat tatgggcata ggaccggccg aagccattcc caagtgtttg aaacaggccg
     1681 gctggaagtt cgaagacgtt gattactggg agattaacga agcttttgcc cctcagttcc
     1741 tgggctgcgg cattcgtctg aaagaaaaat acaacatgac gctggacctg aacaaagtca
     1801 accacaacgg ttccggcatc gctctgggtc atcccattgg cagcaccggg ctgcggatca
     1861 ttgtatccct ctattatgaa atggaaagac tgggcctgac caaaggcggc gcctccctgt
     1921 gcgtaggaac cggaccggcc atggcctctc tctggacgag agatatctag aatagatgac
     1981 ccaatgcaat cgtttgtaga ccctttccct taaaatacgg agctgccggg gttcagcatt
     2041 gacgcatggt tggcgggcca ttgcctcaaa cacactgatc ccggtactcc gaatcccgaa
     2101 ataatcactt ttattatacg ataaattaag gaggttttta taatggattt tcgtttaacc
     2161 gaagaacaag aaatgatcag acaaatggca gccgattttg ctgccaatga aatagcacct
     2221 tatgccatcg aatgggataa agctgactca gtaccctttg atacggtagc caagatgcag
     2281 gacctgggcc tgatgaccat cggtattccg gcagaatacg gcggagacgg actggactgg
     2341 ttatccaaat acctggtcat cgatgaaatc ggccagggcg atgccggtct gggaacgatg
     2401 gtcgtcgcct cctcgctgtt ggcttccgat cccatctggg taggtgccaa tgacgagcag
     2461 aaaaaatggt ggtatggccg tatgctggaa ggcgcagtct gcggattctg cctgactgaa
     2521 ccgggcgccg gttcagatgc cctgggcatg tcctgcaaat gcgttaaaga cggcaatgac
     2581 tatatcctca acggaaccaa acaattcatc accaatggcg gatttgccaa gaacttctct
     2641 gtcttcgcca ccctggataa aaagatgggc aacaaaggac tgtgtgcttt aatggtcgac
     2701 agagacttcc ccggcgtttc catcggacct aaagaagaca aactgggcat caggagcacc
     2761 agcaccaccc aggttatttt cgaagatgtc agagtccccg gctggatgat gctgggcggc
     2821 gaaggcaaag gcactcccat tctgatggag accctggacc tttcccgtac cgccatcgct
     2881 gccctggcaa ccggcatcag caccgccgcc ctgaaagcag ccttggccta tgccaatgaa
     2941 agacaacagt tcggcaaacc aatttccact ttccaggcga tttccttcat gctggcggat
     3001 atggccactg ccgttgaaac ttcccgcctg ctttatcaaa aggcggcctg gctgcaagac
     3061 aatgatctgc cgttcaagat gccttccaac atggccaaaa tgtatgctgc cgattcagcc
     3121 atgaaagtta ccgttgacgc tgttcaggtc tttggcggat atggatacac caaagaatat
     3181 ccggtcgaaa aatacatgcg tgatgccaag atcatgcaga tctttgaagg aaccgtccag
     3241 gtacagagaa tggttatcgg caaatatctg agccagaacg gctacaatcc taatgtataa
     3301 ttccaaacag ccaaatataa tttgcaatcc attcgctaat cactaattcc ttattttctt
     3361 ccctccctcg cccgggctga ccctgcccgg gcactttttt tacaaaaaaa aaaggacctc
     3421 tccgaggtcc ctctggcaaa acgaataaac aaggctgctc atgggctttt acttgctcta
     3481 ttcgtccgcc tcgcagcggg atacgttgtc aagctgtggt ctggtctctg tttttatttt
     3541 agcccatgac cttacccggc gatggctgga cgaatctctt tcaattcctg taaacgattg
     3601 atttttgacg gtccggctcc atatttaatc aacgccccaa cggctaccct ggaacaaaaa
     3661 ccgctcaccc attcctgact ggttgaacgc ggcaaaacac aagccaaaac attattgtac
     3721 cggccctgca gattcgcaga cagaagtact cgatcccggc tgcgctccat tctgatccag
     3781 tcgccgtgtt tcattggcgt acacttcaac tgaccggtgg cgctcttgcg gtagtccagc
     3841 acggccgagt catccgctgt cagacgctgc cagtattgcc tcagatccac cggccgcagt
     3901 gatctgttcc tgcccatcaa gcgaaaatcc tgcagacccc agagatgatt gcgccctcct
     3961 gaatacttct catcaaggca tatgatgcct ttcaaggtag gcatctggct gcgcaaagat
     4021 attgctctgt tgagcatcgc caggttttcg acgaacaaat atttaatatc tgagcggttg
     4081 ataataaatt tcagttcgtc gtcatccacc agcggtgtaa tcgtattgac caccgccccg
     4141 cacatcaata tacccatatt gatccagccg cgctgcgccg aaacctggga catgaccgca
     4201 atgtcatccc ctttgcggac cccgcgccga atcagggcaa cggcaatttc ctctgcaatt
     4261 ccataccatt ctgcatagct gacggttttc cactcctggc atgctttata agtattggcg
     4321 ggtttgctgc cgtgatgctg taccgtatga taaaacatct gcggaattgt gggtacctct
     4381 gtcttgaaac ggtcaagaat ttcgattccc tcacccaaaa ctgctttcat cctttccttt
     4441 cccctttctt ctttaatccc tctctctatt gcactttgtt gcaagccaca tcagcctgaa
     4501 caaatttaca ttcacgagca tattttccat ctctctatca taattatata tttacgtaat
     4561 aaaaatatca aattatatat gatacgtaat ataatattac atatcatgtt attatcatac
     4621 caaagtgttt tgggggactg tccccgaaaa tcactcgaaa ataaaaaaag ggaggcattt
     4681 gcctccctta aaatccggat cgtcctgatc gattagctga ctttgttttc caacctcaat
     4741 ttgtcggcta taatggcgat gaactctgag ttggtgggtt tgcctttttc gccgctgatg
     4801 gtatagccaa agaacttgtt gattttatca tagtcaccgc ggtcccaggc caattcaatc
     4861 gcgtgtctta ttgctctttc cacccggctg ggagtggtgt cgtatttctg ggcaatggtg
     4921 ggatacaatt ccttggtcac cgcccccaga taattgattt cgtcaactac cagcatgatg
     4981 gcatcgcgta aatactgata gccttttaca tgagccggca ccccaatttc atggatgatt
     5041 ttggttactt caacttccag atccttgggc actgaactgg gcatggagat ggtcatggaa
     5101 ctgcctttgg gcgtcttcat tttcacatct ctggcaacct gtttaatcct ttcccctaaa
     5161 acctgcagat taaaaggttt caatatataa taatcagctc ccaattgcac tgccttctgg
     5221 gttatgtttt cctggccgaa tgcggtcagg ataaccacct ttggtctcgg attaagattc
     5281 atttcgttga gcttttccaa caccccaatc ccgtctacat agggcataat gagatcgagg
     5341 atcagcacct caacctcttt tttggcgatg acatccaata cttccattcc gttcgcacat
     5401 acatcaatca attcgaaatc tggatcgttg ctaaaatagt cgcgcagtat gccacaaaat
     5461 tcacgattat catcagccac aagaacttta atctgattac cttcagcaaa catttttgta
     5521 aatcccccct ctttacaaaa tagctcaagt atatttaact taatagcctt tttcgacgat
     5581 tagctcagtg aatcctcttt ttgcattaca ctaaattagc cgaattaatt ccacggcttg
     5641 ctataaaaat acatcattaa acataaatac cagagaagct cttcaaagtg gttttaagct
     5701 atcaccgtgt gaacttgatt caactgcttc cacccggcct gcaaattgtc gaaatgtttt
     5761 ttgggaacta accattgcgg gcatttctga caacatgctg tccataaaaa tcccgtaacc
     5821 cgaagtggga tcatttaaaa atacatgggt aacggcaccg acaattcgat tgtcctggat
     5881 aatcggactg cctgacatgc cctgaacgat gccgccggaa atgcttagaa gccggggatc
     5941 ggtcaccttg atgatcatgc ctttgccgtt ttcccgctgg ggaaatactt tttcgatttg
     6001 aatatcccat ttttcaatgg tattgccgtt cacgaccgtc cacatttggg ccgccccgac
     6061 ctgaacctga tgcgcatagg caacttccaa ttggggagtg gtcggctgaa aattcttgct
     6121 attattgatt ataccataga ttcctgcatc ggtatttttc aatattttac cggtaaacgc
     6181 cccggcttca tcaaaaacac cgattttttc tccgggatga ccaggtttcc cggctctgat
     6241 catctgaatg gaagcagtta aaagcttgcc gcttaacacc tcaatgcctt gtttggtgtc
     6301 agcatccatg atgacgtgac ccaaagctgc aaactgtccg ttcccgggat caataaacgt
     6361 caaggtgccg acgccgacca cgccgtctct gacataaagg ccaatccggt agcgattggt
     6421 ctcagaacaa aattctgccc gcaccggaac ggagacagtt tttccccgcc gctgcagctt
     6481 aacctccaag gactggtttt ttttgctgtc gatcaccttg gccaaatctt cctcattggt
     6541 aataggacga tcgtccacct gataaatcaa gtcaccgatt tcaatcccct gctgcttgcc
     6601 gggatagact ttgctgccgt ccgtctggga gatgggagcg aagcccacca ccatgatgcc
     6661 ccgggattgc agcatgaccc cgatggaatg accgcccacc accactcttt tattgggcag
     6721 ggcttcaacg gcgatcgatt tcaagggaat atagcccagc aatttcatgc tcagctcggc
     6781 tctgcccggt tgcagggcct tgatttcata aacagatcct tttttttgaa ccgtaaccgc
     6841 gggataggat tgcagcagaa aaacccgccg ggtatgctca agaacggtga aatcgatttg
     6901 attctgaagg gttttgggca gactcaaacg aatggcattg gcctgtccca ccacgatttt
     6961 ttgtttttcc ggcagactga acagggtcct ggcctgggga ctgcagcaca agcagagcaa
     7021 caatactgcc aggacaatcc cggccagctt tcgatatgaa tacatatcat ttcctccgca
     7081 gattgatgaa ctttattctt tccgtccggg aggagaaata tacgaagcag gcctcatttc
     7141 ggcatagaaa aactgccggg gaaaaggctc accgacagtc accgaccagg atacaattcg
     7201 gttccgatgt ttttatttgc tacgatggtt catcagttcg cgggcatgct ccagcgttag
     7261 ttctgtataa tcatcgccat ccagcatgcg tgccagttcc ctgatccgct ccggctcgtc
     7321 cagctgggca acccgggtaa aggtacgccc gttttcaaca tacttgtcga tatgtaaatg
     7381 ctgatccgca taactggcca attgcggcga atgcgtaacc aaaatcaatt gatgcctggc
     7441 cgccaaccgg gaggcttttt gggctacggc agtcaaggct gtgcctccca gtccggcgtc
     7501 gatttcatca aataccaggg tgggaaactg atatttttcc gccagggatg ttttcagggc
     7561 aaaaatcaac cgtgaaattt cgcc
//
LOCUS       DAOTHZ010000090         7572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11649_length_7572_cov_5.902887, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000090 DAOTHZ010000000
VERSION     DAOTHZ010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_7572_cov_5.902887"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="P5549_09035"
     CDS             complement(<1..590)
                     /locus_tag="P5549_09035"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HRW13255.1"
                     /translation="MIDLKYHIVSIVAIFIALGMGILIGSTIVGNDVMVAQQQKIISS
                     LERQFADLREHESKLMAEDVKKSKMITQYENFAQSMLVPVVTGQLKGRNVAVIVSGNQ
                     AMPSGIINTLGLAGANLNSQAMVLENINMKDSILRSRLIDYFALEYNTTSDVLRQQVA
                     QSLALQVNNRADPALNVFLEENKLVKMTYSNDQPIDT"
     gene            complement(641..1759)
                     /gene="steA"
                     /locus_tag="P5549_09040"
     CDS             complement(641..1759)
                     /gene="steA"
                     /locus_tag="P5549_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HRW13256.1"
                     /translation="MHIKGRAKLDRRTKNLVTRLRKGDIAIISHDDIDEVAANNLIDI
                     KPQAIINTQPSITGRYPARGALKILKAGIPILDEVPDQIFDNIKEGNWLQIEDNKIIS
                     GRLLLAEGRVLTLEDVQEKLEQANLNFGHQLDDFVDNTLEYAKKEKDVLLGNLQVPEL
                     DSVLLGRHVLIVVRGSSYKEDLRAISTYIQEQKPVLIGVDGGADALLEFRLQPDIIVG
                     DMDSVSDQALQCGAEIVVHAYANGKAPGLERVQAMGIEPVIFPVTGTSEDAAMLLAWE
                     HGAKLIVAVGTHSNMVDFLEKGRKGMASTFLVRMKVGSILVDARGVSQLYQQSIHRRY
                     FAELFLAALVPIAILLWVSPATKPFFNLLWLQIKLLFN"
     gene            2014..4122
                     /locus_tag="P5549_09045"
     CDS             2014..4122
                     /locus_tag="P5549_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW13257.1"
                     /translation="MPNTVYFELSHVMDAMIENPHMFFVVVNAEGKLISISQTLLRLL
                     DTTEEQVLGKHILDVIPDGKLPEVLKTGRIDPGDVLWVNGHKTVVTRVPIVKDGEIVG
                     AVCSSLFMDILAAQDLVNRFNAPGAAPELDIVINELIDSPSSGYIIIDINGIITHINQ
                     TYLNIIGKKRKEVIGQPIMKITPHSRLPEIVKTGKIDTVDVWSVNGQNMLVNRLPIKH
                     NGKIVGAIGHTLVLDTSVHQFLMEKLQETDDLLFEGLIENPHAGYVTVDNQGIITSMN
                     RTLMEALHLNSLSVIGRYILDVIPNSKLPEILATGRTDKTDIWSIAPAREVIVDRMPI
                     KKDGEIIGAIAHTVIMDMSEVKYLIQRLQDTQQELNIYKDAVCSIYNARWHFSNLIGR
                     NIDFLNIVTMAQQFSQTSSTLLITGESGTGKELFAQAIHNASPRSAGPFIRINCAALP
                     ENLLESELFGYAEGAFTGAKKGGKPGKFELANGGTIFLDEIGDMPMNMQTKLLSVLQE
                     RVVERVGGTTPIQINARVIAATNRDLEEMVAKHEFRDDLYYRLNVVQLKLPPLRKRMD
                     DLPVLVSNLMIRLNKKLGTSVEGISAAAMDLLRSYPWPGNIRELENLLERAINLAHMN
                     FHDSIQPSDFPSLNSNQAVNHSIDIDESKSLAERMDDIEKEMIVKALEKTNDNKSQAA
                     KLLGMHTSALYRKLSKYNIS"
     gene            4604..5446
                     /locus_tag="P5549_09050"
     CDS             4604..5446
                     /locus_tag="P5549_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRW13258.1"
                     /translation="MQKVGLLGAGTMGQGIAWALAQSGYEVILYDIKQEFCDKAVAII
                     GKGLAKLEEKGKAPAGTKDAVVSKIKPTGNFDDLAASDFVIECVFENMDVKKDVYTRL
                     DALLKPDVIIATNTSSLSITEIAAITKRADKIAGMHFFNPANVMKLIEVIPALQTSEE
                     TIEVVMELSKAIGKTPIKVLEGPGFVVNRILVPGMNEACGILFEKLATAEDIDTAMKL
                     GANWPMGPFALCDLVGADISLAVLNTLYNETGDPKYRPSTLLKQHVRAGWLGRKTGRG
                     FFQY"
     gene            5495..6277
                     /locus_tag="P5549_09055"
     CDS             5495..6277
                     /locus_tag="P5549_09055"
                     /EC_number="4.2.1.150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain-enoyl-CoA hydratase"
                     /protein_id="HRW13259.1"
                     /translation="MAYENVILEKENDLAILTVNRPKALNALNKDTLLDIQAAVAEVS
                     NDPAIKVLIITGSGEKAFVAGADIAYMKDFNVREGRAFGQLGQQVFRSIELMEKPVIA
                     AVNGFALGGGCELALSCDIRLASENAVFGQPEVALGVTPGYGGTQRLPRLIGEGLAKE
                     LIFSSSNLKAYDAYRVGLVNHVYQAAELMAEAKKLAAKIAQQAPLAVANSKTAINRGI
                     QVDLDTAMQIEADLFGMCFATEDQKEGMRAFVEKRKPAFKAQ"
     gene            6353..>7572
                     /locus_tag="P5549_09060"
     CDS             6353..>7572
                     /locus_tag="P5549_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13260.1"
                     /translation="MDYSAEYKSKLITAEQAAQLVKSGDWLQYGEFIMQPKDCDAALA
                     KRKDELKDVKIRVTTMSWLPEVCKVDPEKEHFIMNDWHFSGTSRKLMDMNLCYYIPFT
                     YHEVGDIYNDFLDVDVSFQPVTSMDKNGFFNFSTANSMASYSTKKAKKLVVEVNSSTP
                     VCLGGMGESIHISQVDHIVESQTNPKLANLPELPVTDTDRRIAAHVMELMCDGACVQL
                     GIGSMPNTVGALIAGSDLKDLGVHTEMLCDSFVDMYNAGKITGARKTLDKGKMVYTFA
                     MGTNKLYEFLDHNPVCASYPVHYCNDPHVIAQNDNVMAINNCLEVDIYGQVASEASSG
                     RQISGTGGQLDYIIGAFHSKGGKPIIALSSTVKKSDGSLASRITPGLSPGTIVTVPRS
                     ITSYVITEYGGVNL"
BASE COUNT         2055 a         1907 c         1726 g         1884 t
ORIGIN      
        1 gtatcgatgg gctgatcgtt gctatacgtc atttttacca atttgttttc ttccaaaaaa
       61 acattcaggg ccggatctgc ccgattgttc acctgcaaag ccagtgattg cgcgacttgt
      121 tgtctaagta catccgaagt ggtgttgtat tccaaagcaa aataatcgat caaacgggaa
      181 cgcaagatgc tgtccttcat attgatattt tccaatacca tcgcctggct gttaaggttg
      241 gctccggcaa gaccgagggt attgataatt ccgctgggca tggcctgatt tccggaaacg
      301 atcaccgcta cattccgtcc ctttaattga ccggtcacca ccggcaccag catactctgg
      361 gcaaaatttt cgtattgggt aatcattttg ctctttttta catcttctgc catcagtttg
      421 ctctcatgct cccgcaagtc agcaaactgc cgctccagac tgctgatgat cttctgctgc
      481 tgcgccacca tgacatcatt gccaacgatc gtactgccga tcaaaatgcc cattcccagg
      541 gcaatgaaaa tcgccacaat cgaaacaatg tgatacttta aatcaatcat ttccatccca
      601 ccgttatctt taatctgtta tccaatgaac cgtaactcat ttagttaaac agcagtttaa
      661 tctgcagcca caacaagtta aaaaaaggct tggttgctgg agaaacccac aatagaatcg
      721 caattggaac cagcgcggcc aggaacaatt cggcaaaata acgccggtgt atactctgct
      781 gatacaactg gctaacgccc cgggcatcca ccagaattga acctactttc attctcacca
      841 aaaaagtgct ggccatgcct ttgcggccct tctccagaaa atcaaccata ttggaatgcg
      901 tgcccaccgc gacgatcaat ttggctccgt gttcccaggc caacagcatg gctgcatctt
      961 cactggttcc cgttaccgga aaaatcaccg gctcgatccc cattgcctgt accctctcca
     1021 aacccggcgc cttgccgttg gcataagcat gaacaacaat ttccgccccg cactgcagcg
     1081 cctggtcgga aacactgtcc atatcgccca caataatatc cggctgcaaa cgaaattcca
     1141 gcaaggcatc cgctccgccg tcaacgccga tgagcactgg tttttgttcc tgaatatagg
     1201 tactgatcgc ccgcaaatcc tctttatagc tgctgccgcg gactacgatc aagacatgcc
     1261 gtcccagcag cacgctgtcc agctccggaa cctgcagatt gcccagcagg acatcctttt
     1321 cctttttggc atactccagg gtattgtcaa caaaatcatc caattggtga ccaaagttga
     1381 gattggcctg ctccagcttt tcctgcacgt cctccaaggt tagtactcgg ccttctgcca
     1441 gcagcaatct gcccgagatg attttgttgt cctcaatttg cagccaattg ccttccttta
     1501 tattatcaaa aatctgatcg ggcacttcat ccaggatagg gataccggct ttcaaaattt
     1561 tcagcgctcc gcgagccgga taacgtcccg taatggaagg ctgggtattg ataatcgcct
     1621 ggggcttgat atcaatcaga ttgttggccg cgacttcatc aatgtcatcg tgactaatga
     1681 tggcaatatc gcctttgcgc agtctggtca ccagattttt ggttcgcctg tcaagcttgg
     1741 ccctgccctt tatatgcatg gtatacctca agcaaaaaat agtacggtct tttacaaatc
     1801 tatactaaca tattgtacca ggtaaacaaa atccctggat tgtatgatat cataaacagt
     1861 tgttcgctag tgaaaaaccg ggcaagccaa atctgcagag cttgtgaatt atttatctat
     1921 caatgttgac aattacctag aaatgtttca gtatttaaac atatattata atattgtaga
     1981 aatttgacgg tgtcattcgg gaggtaaatt ccattgccaa acacagttta ttttgaacta
     2041 tcccatgtaa tggatgccat gatcgaaaat ccccacatgt tttttgtggt cgtaaatgct
     2101 gaaggcaagc tcatcagtat cagccagacc ttgttgcgtt tgctggatac aaccgaagaa
     2161 caagtcctgg gcaaacacat tcttgacgta atcccggacg gaaaactccc cgaagtcttg
     2221 aagactgggc gcatagaccc cggcgatgtt ctctgggtaa atggccacaa aaccgtggtt
     2281 acccgggttc cgatcgtgaa agacggggag atcgtcggtg ccgtttgcag cagtttgttt
     2341 atggatatat tggcagccca ggatttggtc aaccggttca atgcgccggg agctgcaccg
     2401 gagcttgaca ttgtcatcaa tgaattgatc gacagtccct cctcgggcta tattattatc
     2461 gatatcaacg gtattattac ccatatcaac caaacctatc tgaatatcat cggtaaaaag
     2521 cgcaaagaag tcatcggtca gcccattatg aagatcaccc cccattcaag actcccggaa
     2581 atcgtaaaaa ccgggaaaat cgatacagtc gacgtatggt ctgtcaatgg tcaaaacatg
     2641 ctggtcaacc gtttgccaat caaacataac ggtaaaatcg tgggggcgat tggccatacc
     2701 ctggtcctgg atacttcggt tcaccagttc ttaatggaaa aattacagga aacagacgat
     2761 ctgctctttg aagggctgat tgagaacccg catgccggtt atgtaaccgt cgacaatcaa
     2821 ggaatcatca cgtcaatgaa ccggactctg atggaagctc ttcatctcaa cagtctgagt
     2881 gtaatcggca gatatatcct ggacgtgatc cccaactcca aactccccga aatactggct
     2941 accggcagga cggacaagac ggacatctgg tcgattgctc ctgcacggga ggtcatcgtc
     3001 gatcgtatgc cgatcaagaa agacggagaa atcattggcg ccattgccca taccgtcatt
     3061 atggacatgt cggaagtgaa atatctgatt cagcgtttgc aggacaccca gcaggaactg
     3121 aacatctaca aggatgcggt ttgctcgatt tataatgcca gatggcattt cagcaacttg
     3181 atcggccgca atattgactt tttgaatatc gtgaccatgg cccagcaatt ctcccagacc
     3241 tcttccacct tgctgattac cggcgaaagc ggcactggca aggaactgtt tgcccaagct
     3301 attcacaacg ccagccctcg cagtgcggga ccgtttattc gcatcaactg tgccgctctg
     3361 ccggaaaacc tgctggaatc tgaactcttc ggctatgccg aaggagcctt taccggggcc
     3421 aaaaaaggcg gcaaacccgg caaatttgaa ttggccaacg ggggtaccat ttttctggat
     3481 gaaattggcg atatgcccat gaatatgcag actaaactgc tgtccgtgct ccaggagaga
     3541 gttgtggaac gggttggagg aaccactccc atccaaatca acgcccgtgt catcgctgcc
     3601 accaatcgtg atctggaaga gatggtcgcc aaacacgaat ttcgcgatga tctatattat
     3661 cgtttaaatg tcgttcaatt aaagcttcct cctttgcgca aacgcatgga cgatctaccc
     3721 gtactggttt ccaatctgat gatcagactc aataaaaaac tggggacttc tgtagaaggc
     3781 atttccgccg cagccatgga tttgctgcgc agttaccctt ggccgggcaa tattcgcgag
     3841 ttggaaaacc ttctggaaag ggccattaat cttgcccata tgaattttca cgacagcatt
     3901 cagccgtctg atttcccctc cttgaacagc aaccaggcgg ttaatcattc gattgatatt
     3961 gatgaatcca aatctctggc ggagagaatg gatgatattg aaaaagaaat gatcgtcaaa
     4021 gccctggaaa aaaccaatga caacaaatcc caagcagcca aactcttggg catgcacact
     4081 tcagccctgt acagaaagct cagcaaatac aacatcagtt aagcctcttc agatattaat
     4141 acccgccggc cggagcatgg agaactgatc gcagtatcaa aatgctcctt ctttacgact
     4201 gtcacaaaac tgtctcacgc caaccctccg gttttgcact tttgtgatag tcctctccca
     4261 ttttgataaa atgccctctg tgctccattt cacaagcttt tttcctttgc ccttcccgtc
     4321 aacgattgtc tttttccggg ttactttccc attttgtaat agtaggccga ttttctgtcg
     4381 gattttagca caaacctcaa atccctatac agcaaccttt ttcattgttt ttttcattct
     4441 tgttattttt ggcacaatac ttgctctata tattcgacag aatcaaagag cgaaaacaat
     4501 ggtaaggggg ggctgcctgg acaagcataa tttgcggcaa gaaaagtaac aaacattacg
     4561 attaccagca atgataaata agaaggaggc aaacacaatg gcaattcaaa aagtaggatt
     4621 attaggcgca ggaacaatgg gacaggggat tgcatgggca ctggcacagt cgggctatga
     4681 agtcattctc tatgatatta aacaagagtt ttgcgacaag gcagtggcca tcatcggcaa
     4741 aggtttggcc aagttggaag aaaaaggcaa ggctcccgcc ggaaccaaag acgcggttgt
     4801 cagcaaaatc aaaccgaccg gtaattttga tgatctggcc gcatctgact ttgtcattga
     4861 atgcgtattc gaaaacatgg atgtcaaaaa agatgtctat acccgtctgg atgcgctgct
     4921 caaacccgat gtcatcatcg ccaccaatac atcatctctg tcgattacag aaattgcagc
     4981 catcaccaag agagctgaca aaatcgccgg tatgcatttc tttaatccgg ccaatgtcat
     5041 gaaactgatc gaagtcatcc cggctctgca gacttccgaa gaaaccattg aagtcgtcat
     5101 ggaattgtcc aaagcaattg gcaaaacccc catcaaggtt ttggaaggtc ctggctttgt
     5161 cgtcaatcgt attctggttc ccggaatgaa tgaagcttgc ggaatcttat tcgaaaaact
     5221 ggccaccgcc gaagatattg atactgccat gaagcttggc gccaactggc cgatgggtcc
     5281 ttttgctctc tgcgatctgg tcggggcaga catctctctg gctgtgttaa acactctgta
     5341 caatgagacc ggcgacccca aatatcgtcc ttccaccttg ctgaaacagc atgtacgggc
     5401 cggctggctg ggacggaaaa ccggacgggg attcttccag tactaagatg caaaggttcg
     5461 cacacccaaa cctaccggac agggggacaa gaatatggct tatgaaaatg taattctcga
     5521 aaaagaaaat gacctggcga ttttgaccgt caatcgtccc aaggcactta acgccctgaa
     5581 caaggacacc ctgctggata ttcaggcagc cgttgccgag gttagcaacg atccggccat
     5641 aaaagttctg atcattaccg gcagcggtga aaaagctttt gttgccggag cagacatcgc
     5701 ctatatgaaa gattttaacg tccgcgaagg ccgtgccttt ggccagttgg gccaacaggt
     5761 attccgctcc attgaactga tggaaaagcc cgtcatcgct gcagtaaacg ggtttgcatt
     5821 gggcggcggc tgtgaactgg ctttgtcctg tgacatcagg ctggcatcgg agaatgccgt
     5881 gtttggacaa ccggaagtag ccctgggcgt cacccccggt tatggtggaa cacagcgtct
     5941 gccccgcctg attggggaag gtctggccaa ggaattgatc ttcagttcct ccaacctgaa
     6001 agcctatgat gcatatcggg tgggtctggt caatcatgtg tatcaggccg ccgagttaat
     6061 ggccgaagcc aaaaagctgg cagccaaaat tgcccagcag gcaccattgg cggttgccaa
     6121 ttccaagacc gccatcaaca ggggcataca ggttgatttg gatacagcca tgcagattga
     6181 agccgattta ttcgggatgt gcttcgctac agaagatcaa aaggaaggca tgcgggcgtt
     6241 tgtagaaaaa cgcaaacccg ccttcaaggc tcaataatca tagcaccaaa gcaatcgctg
     6301 taaccaggtc agccagggaa atcaatcatt gaaaacaagg aagtgataaa gaatggatta
     6361 ttcagctgaa tataaaagca agctcatcac cgcagaacaa gcggcccaac tggtcaagtc
     6421 aggcgactgg ctgcaatatg gcgaattcat catgcagccc aaagattgtg atgccgccct
     6481 ggccaaacgc aaagacgaat taaaagatgt aaaaattcgc gtgaccacta tgagctggct
     6541 gccggaagtc tgtaaagtgg atccggaaaa agaacacttt atcatgaatg actggcattt
     6601 ttccggtact tcccgcaaac tcatggacat gaatctctgt tattacattc cctttaccta
     6661 tcatgaagtc ggtgacattt ataatgattt tttggacgtg gatgtaagtt tccagccggt
     6721 cacctcgatg gacaaaaacg gcttctttaa cttcagcact gccaactcaa tggcttccta
     6781 ctcgaccaag aaagccaaga aactggtggt cgaagtcaat tcctccaccc cggtgtgcct
     6841 gggtgggatg ggcgaatcga ttcacatttc ccaagtggac catatcgtag aaagccaaac
     6901 caacccgaag ctggccaatc tgcctgagtt gccggtaacc gatacggacc gccggattgc
     6961 tgctcatgtg atggaattga tgtgtgacgg tgcctgtgta cagcttggga ttggttccat
     7021 gcccaacacc gtgggggccc tgatcgctgg atccgatttg aaggatctgg gcgtccatac
     7081 cgagatgctg tgtgattcct tcgtggatat gtacaatgcc ggcaagatca ccggggcaag
     7141 aaaaaccctg gataaaggca aaatggtcta tacctttgcc atgggaacca acaagctcta
     7201 cgaattcctc gatcacaacc cggtatgtgc cagttatccg gtgcattact gcaatgatcc
     7261 gcacgtgatc gcccagaatg acaatgtgat ggccatcaac aattgtctgg aagtcgacat
     7321 ttatggacag gtcgcctccg aagcttcatc cgggcgacag atttccggta ccggcggaca
     7381 attggactac attattggcg cttttcattc caaaggcggc aagccgatta ttgccctgag
     7441 ctcgacagtc aaaaagtcgg atggaagcct ggcttccagg atcactcccg gcttaagccc
     7501 gggcacgatt gtaactgtcc cccgctccat tacttcttac gtgattacag agtatggagg
     7561 cgtcaatctg aa
//
LOCUS       DAOTHZ010000091         7563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_11662_length_7563_cov_6.538625, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000091 DAOTHZ010000000
VERSION     DAOTHZ010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11662_length_7563_cov_6.538625"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /gene="era"
                     /locus_tag="P5549_09065"
     CDS             complement(<1..388)
                     /gene="era"
                     /locus_tag="P5549_09065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRW13261.1"
                     /translation="MTVKSGFVSIVGRPNVGKSTLLNTIIGEKIAIVSDKPQTTRTRI
                     QGVHTCDEGQIIFVDTPGIHKPKHLLGEYMVKVSTRSLDEVDLIYYMTDVTRPYGGGE
                     QYIIEQLKSTRVPIFLLVNKIDLVEEE"
     gene            complement(419..802)
                     /gene="cdd"
                     /locus_tag="P5549_09070"
     CDS             complement(419..802)
                     /gene="cdd"
                     /locus_tag="P5549_09070"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRW13262.1"
                     /translation="MEAAKLVSEARRAQQNAYAPYSRFQVGAALLTADGQVYLGANIE
                     NSSYGLTVCAERNAVFKAVYEGKRNFTAIAVCASGSGYVYPCGACLQVLAEFSPQIRV
                     IVSNENSEYREYELKDLLPQIFLLS"
     gene            complement(821..1174)
                     /locus_tag="P5549_09075"
     CDS             complement(821..1174)
                     /locus_tag="P5549_09075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRW13263.1"
                     /translation="MGKGTLRDSFGYALRGILAAVASGRNMKIHLLATIMAVITGWLL
                     GINRLEWAMITISIFMVLAAETLNTAIEKAVDLVTREYHPLAKQAKNLAAGAVLLTAI
                     SAVIIGLLIFGPYLW"
     gene            complement(1184..1654)
                     /gene="ybeY"
                     /locus_tag="P5549_09080"
     CDS             complement(1184..1654)
                     /gene="ybeY"
                     /locus_tag="P5549_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRW13264.1"
                     /translation="MDTMIINQQDKVNYNRDVQQVILKVVKTIAKLGSIPKNTELSIL
                     LVDNSYIKDLNYVYRHVNSATDVLSFAMNELSEGEPAFTFTNEVNVLGDIVISLEKAQ
                     EQSKEYGHSFERELGFLVAHGLLHLLGYDHENETEAKVMQELEKKILQAAKLER"
     gene            complement(1657..3813)
                     /locus_tag="P5549_09085"
     CDS             complement(1657..3813)
                     /locus_tag="P5549_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRW13265.1"
                     /translation="MKLSNLFTYFFADKLAGIWKNQSVRKMLALAFFFSVVFMTLFSS
                     LTPQQVSLRIDEVSKNDIVSEINAVVIDEKQTAELRQQAASRVQKVYQEDNYALVNSR
                     DEVTAIYASLKEVQQSGKATRKDDLQKILEAAENPNHPLNRDDKQLTQYLLNATLDDL
                     EQMEKTTFSVIERAMGKPITDEALEDAYKEIALQINQLGFAAEAQDIMGLIAIKTVRP
                     NMLYDQEATERAMNEARTQVKPVQKNIKAGEIIVREGERVTAEQISVLEQLGIQRSKN
                     YSLTLVGTILFILVFFFVWLEYIKLNHRKIYHNEKLMLLLGLIFVLIILLSRLVTIVE
                     IPNRPNINAMTGYLAPVAAGSMLIAILIDNKLAYFMTVVMAIFIGMMFGGNQLAYTIV
                     AAVGGTIGVYRVSNLSQTSDLARAGLYLAAANIITIITMSLLAGKFGLNMAVLAVIIG
                     TTSGLLSAILMIGVLPYLESAFSITSMIRLLELSNPNQYLLKRLLLEAPGTYHHSLMV
                     GNLAEAAAEAVGANALLVRVGSYYHDIGKIRRPEYFVENQQGNINPHAKIAPALSALI
                     ITSHVKEGLEMGREQHLPQSIMDFIAGHHGTSLTKYFYGRALEEDGGDNINEDNYRYI
                     GPKPQSKEVALVMLADAAEAAVRSISEPTSEKIRDMVRKIIRDKLNDGQLEECDLTFR
                     DLDIIAATFVRVLEGIYHRRVEYPEIIAEELTRRRV"
     gene            complement(3903..4865)
                     /locus_tag="P5549_09090"
     CDS             complement(3903..4865)
                     /locus_tag="P5549_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRW13266.1"
                     /translation="MSIKEAKIKITDNQQAAIFFGTGDVNFKHLQAISGADIVARGSE
                     LSIRGEEEAVDHTVKLIHGLIDILENEKALNINDITYVDSLLQRGDAPRHQDIVSATI
                     LSTARGKAIKARTIGQKKYIQAILKDDIVFSIGPAGTGKTYLSVVMAVRALRNKEVER
                     IILVRPAVEAGEKLGFLPGDLIEKVNPYLRPLYDSLFDILGVDVTQRYLEKNIIEVAP
                     LAYMRGRTLNNAFIILDEAQNTTPQQMKMFLTRLGFGSKMVITGDITQVDLPADEQSG
                     LIEIQKVLCSIEGISFEYLGKEDVVRHPLVQKIINAYELLEKHF"
     gene            complement(4939..6144)
                     /locus_tag="P5549_09095"
     CDS             complement(4939..6144)
                     /locus_tag="P5549_09095"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HRW13267.1"
                     /translation="MINRFFNHLGGVITLRIKGKNPEQLINLALSRGVYIWDVKWQGE
                     NLRMCIRNSALPAFQAIAAENGFALEIVSRRGWPFFKTKAQRRIGFFGGAALFVLILY
                     GLSLFVWFVDVEGNQVVSTHQILQSAARHGLYQGSGKWQISCSEAEKAILRELPRLTY
                     VQCEIQGARVHIRVVEKVWPDEEFLGPCHLVAKRDGVIEEVLLLQGQAIVKTGQIVGR
                     GDILISGIVYPPEPEPTELLDNEPAKQPALVRARGIVKARTWYEGYGECPRSIEKRVL
                     SGKTLREIKLITPWKEIRVKDAKISQNGLYQQEGKHFSVNTPPGLWGIYIKQYHEQYK
                     NVKTYTEAQAIVVAREKGINHLRRKIQPGVEISDTRLEVLSSPGDAIVRIKVAIESIE
                     DIGGVQPLE"
     gene            complement(6159..6437)
                     /gene="yqfC"
                     /locus_tag="P5549_09100"
     CDS             complement(6159..6437)
                     /gene="yqfC"
                     /locus_tag="P5549_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HRW13268.1"
                     /translation="MEKSKEMISRAMADLLEIPKDLVLDIPKITLIGRGELYLENHRG
                     IIEYSNQRLRINLSRGFLEILGDHLEIKALLPDEIKIAGDISTCRFVD"
     gene            complement(6679..6918)
                     /locus_tag="P5549_09105"
     CDS             complement(6679..6918)
                     /locus_tag="P5549_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HRW13269.1"
                     /translation="MADIKFLITRELGTLGDVSKGWQKEINLVSWNDRKAKLDIRDWG
                     PGHERMGKGVTLNKEEAVRLKQLLAEMDLDSLDIG"
     gene            complement(7358..7534)
                     /gene="rpsU"
                     /locus_tag="P5549_09110"
     CDS             complement(7358..7534)
                     /gene="rpsU"
                     /locus_tag="P5549_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRW13270.1"
                     /translation="MSEVKVGKNESLDSALKRFKRSCQKSGVLQDIRKHEHYEKPSVR
                     KKKKSEAARKKKKF"
BASE COUNT         1842 a         1997 c         1594 g         2130 t
ORIGIN      
        1 tttcttcctc caccagatct attttattga ccagcaggaa tatcggcact cgagttgatt
       61 tcaattgctc gataatgtac tgttcgccgc cgccataggg ccgggtaaca tcagtcatat
      121 aatagatcaa atctacttcg tccaggctgc gggtagatac cttgaccatg tactctccca
      181 gcaaatgctt gggtttatga attccaggcg tatccacgaa aataatttga ccttcatcac
      241 aggtatgaac tccctggatt ctggtccggg tcgtctgtgg tttatcggaa acaattgcaa
      301 ttttttcgcc gatgatggta ttcagtaacg ttgacttgcc cacattgggt ctgcctacga
      361 tgcttacaaa acctgatttc acagttattt actcctttat tgcacagatt cccctatttt
      421 aggataataa aaagatttgg ggaagcaggt cttttagttc atattcacga tattcgctgt
      481 tttcgttgct gacaataacc ctgatttgcg gcgagaactc agccaaaacc tgcaagcagg
      541 caccgcaggg atacacgtag ccgctgccgc tggcgcacac ggcgatcgcc gtaaaatttc
      601 gttttccttc gtaaaccgct ttaaaaaccg cattacgctc tgcacatacg gtcaaaccat
      661 aggatgaatt ctcgatattg gccccgaggt aaacctgccc gtccgccgtc aacaaggcag
      721 cacctacctg gaaacgggaa tatggagcat aggcgttttg ctgagcccgg cgggcttcac
      781 tgactaactt tgctgcttcc atcgtcttct cctcgttgtg ttaccataaa tacggtccaa
      841 aaatcagcaa accaataatg acggcactga tcgcggtcag cagaacggcg ccggctgcca
      901 aattcttggc ctgtttggcc aagggatggt actcccgggt caccagatca acggccttct
      961 caatggctgt attgagcgtt tcggcagcca gtaccataaa aatacttatg gttatcatag
     1021 cccattccag acgatttatt cccaacaacc agccggtgat tacggccatg atcgtggcca
     1081 gaagatggat cttcatattg cgcccgctgg caactgctgc caggatcccg cggagggcat
     1141 agccaaagct gtcccgtaaa gtgcctttgc ccatctattg attctacctc tccagcttgg
     1201 ctgcttgcaa tatctttttc tccaactcct gcatgacctt ggcctcggtt tcattttcat
     1261 gatcgtagcc cagcaggtgc aaaagcccat gggctaccag gaatcccaat tcccgttcga
     1321 agctgtgccc gtactccttg ctctgttcct gtgccttttc caaagatatt acgatatcgc
     1381 ccagtacatt gacttcattg gtaaaagtaa aggctggttc gccctcgctt aattcattca
     1441 tagcgaagga caaaacatcg gtggcggaat tgacatgacg ataaacataa ttcaggtctt
     1501 ttatataact attatctacc agaaggatgc taagctctgt atttttgggt atactgccta
     1561 atttggcgat ggttttgacg actttcagga tgacttgctg cacatcccga ttataattga
     1621 ccttgtcctg ctggttgata atcatcgtgt ccatggttaa accctcctcc ttgtcaattc
     1681 ctcggcgata atttcaggat actcgactct tctatgatat atcccctcca ggacccggac
     1741 aaacgtcgct gcgatgatgt ccaaatcccg aaaagtaaga tcacattctt ccagctgtcc
     1801 atcattcagc ttgtcccgaa taatcttgcg caccatgtcg cgaatcttct cactggtagg
     1861 ctctgaaatg gagcgaacag ctgcttctgc agcatcagcc agcataacca gggcaacttc
     1921 tttgctctgc ggtttgggtc cgatataacg atagttatct tcattgatgt tatccccgcc
     1981 atcctcttcc agggctcgtc cataaaagta cttggtcagg ctggtaccgt gatgtccggc
     2041 gataaagtcc atgattgatt ggggcaaatg ctgttcccgt cccatctcca atccttcttt
     2101 gacatgggag gtgatgatca gggcactcaa cgccggcgcg attttggcgt gcgggttgat
     2161 gttgccctgt tggttttcta caaaatattc cggcctcctg atcttgccga tatcatgata
     2221 ataagaccca accctgacca acagcgcatt ggcccctacg gcttcagcag ccgcttccgc
     2281 caggtttccg accatcagac tgtgatgata ggtacccggc gcttccagca gcaaacgctt
     2341 gagcagatat tgattgggat tggataattc cagcaatctg atcatactgg taatggaaaa
     2401 ggccgattcc agataaggca ggacgccgat catcaaaatc gctgacaaca aaccgctggt
     2461 cgtaccaata atgactgcca gcacagccat attcagacca aacttgccag ccagcaaaga
     2521 catggtgata atcgtaatga tgttggcagc tgccagataa agcccggctc gggccagatc
     2581 tgatgtctgg ctgaggttgg atacccggta cactccaatg gttccgccaa ctgcggcgac
     2641 aatcgtatag gccaattgat tgccgccaaa catcataccg ataaagattg ccatgacaac
     2701 cgtcataaaa taggccagtt tattgtcgat caggatggca atgagcatgg accctgccgc
     2761 aaccggggcc agataaccgg tcatggcatt aatattgggc cggttgggta tctctacaat
     2821 ggtcaccagg cgagacaaca ggatgattag gacaaaaatc aagcccaaca acagcatcag
     2881 cttttcatta tgataaatct tgcgatgatt cagtttgatg tactccaacc agacgaaaaa
     2941 gaacaccaga atgaacagga tcgtgccgac gagggtgagg gaatagtttt tgcttctctg
     3001 aatccccagt tgctccaaca cagagatttg ctcggccgta actctctcgc cctcacgaac
     3061 aatgatctcc cccgctttga tgttcttctg caccggtttt acctgagtgc gggcttcatt
     3121 catcgccctc tctgtcgctt cctgatcata aagcatgttg gggcgcacgg ttttaatcgc
     3181 gattaacccc ataatatctt gggcttcagc cgcaaaaccc aactggttga tctgcagggc
     3241 gatctccttg taggcatctt ccagagcttc gtcagtgatg ggctttccca tggcccgttc
     3301 aattacactg aacgtagtct tctccatttg ttccagatca tccaaggtcg cattaagcaa
     3361 atactgcgtt agctgcttgt catcccgatt gagcggatga ttggggtttt cagccgcttc
     3421 gaggattttc tgcaagtcgt ccttgcgggt ggctttgccg ctttgctgta cttccttgag
     3481 gctggcatat atggcagtaa cttcatcgcg cgaattaacc agtgcataat tgtcctcctg
     3541 ataaaccttt tgtacgcggg atgccgcctg ctgccgcagc tctgcagttt gtttttcgtc
     3601 gatcaccaca gcattgattt cggatacgat atcattcttg gagacttcat cgattcgcaa
     3661 gctgacttgt tgcggggtaa gactgctgaa aagggtcata aacacgacac tgaaaaaaaa
     3721 tgccagcgcc aacattttcc taacgctctg gtttttccat atgcccgcca gtttatccgc
     3781 aaaaaaatat gtgaaaagat tgctcagctt catgcaacgc tccttcattc tccccagttc
     3841 atggatcaag cctcacccgc aacggttccc gtaaatgttg gaccagcatc ttgacccatc
     3901 ttttagaaat gcttttccag caattcataa gcattaatga ttttttgcac caagggatga
     3961 cgcaccacat cttccttgcc caaatattca aaggaaatgc cctcgatgga gcataagact
     4021 ttttgaattt caatcagtcc ggactgctcg tccgctggca agtcaacctg ggttatatcg
     4081 ccggtgataa ccattttgga gccaaacccc aaacgcgtta aaaacatttt catttgctgt
     4141 ggtgtcgtgt tttgggcttc atccagaatg ataaaggcat tgttgagggt gcggcctcgc
     4201 atataagcca acggtgccac ttcgataatg tttttttcca aataacgctg ggttacatcc
     4261 actcccagga tatcaaacag actgtcataa aggggacgca gatatggatt gactttttcg
     4321 atcagatcgc cgggtaaaaa gccaagcttt tcaccggctt caaccgccgg tctgaccaga
     4381 atgatgcgtt ccacttcctt gttgcgtaaa gcccgcaccg ccatcacgac cgacaaatac
     4441 gtcttgccag tgccggccgg accaattgaa aatacaatgt catctttcag aatggcctgg
     4501 atatacttct tttgtccgat ggtcctggct ttaattgcct tgccccgggc ggtactcaaa
     4561 atggtggccg acacaatgtc ctggtggcgg ggcgcatcgc ctctttgtaa aaggctgtcc
     4621 acataagtaa tatcatttat attgagggcc ttttcgtttt ccaggatatc aatcaaacca
     4681 tgaattaatt ttaccgtatg atcgactgcc tcttcttctc cgcgaatgct taattccgac
     4741 ccccgggcaa caatatcggc accgctgatg gcctgcaaat gtttaaaatt gacatcaccg
     4801 gtcccgaaaa agatcgctgc ctgctggttg tccgtgattt taattttggc ttccttgatt
     4861 gacaagtaat aattcctcct gccagcaaaa cttcatttgg ctacaattat agcatatgca
     4921 caagcaacgt actaactctt attcaagcgg ctgaaccccg ccgatatcct ctatgctttc
     4981 gatggcaact ttgattcgca ctatggcatc gccgggcgat gacaacactt cgagccgggt
     5041 atcgctgatc tcaacgccag gttgaatttt gcggcgcaaa tggttaatcc ctttttcccg
     5101 ggcaaccaca atcgcctggg cttcagtata ggtcttaacg ttcttgtact gctcatgata
     5161 ctgctttata tatattcccc acaaacccgg cggggtattt accgaaaaat gtttcccctc
     5221 ctgctggtac agtccgtttt gagaaatctt cgcgtctttt acccggattt ccttccaggg
     5281 cgtaatcaac tttatttccc gcaacgtctt gccgctcaac actctttttt caatgctcct
     5341 tgggcattcc ccatagcctt cgtaccaggt gcgggccttg acgattcccc gggctcttac
     5401 caaagcaggt tgctttgcag gttcattatc cagtagttca gtcggttcag gctccggcgg
     5461 atacacaatg ccggatatga gaatgtctcc acgccctaca atctgaccgg ttttgacgat
     5521 cgcctggcct tgcagcagca aaacctcttc gatgacacca tcacgctttg ccaccaggtg
     5581 acagggaccc aaaaattctt catccggcca taccttttcc actacccgga tgtgtacccg
     5641 ggctccttga atttcgcatt ggacataggt taaacgaggc agctcacgca ggatggcttt
     5701 ttctgcttcg ctgcagctga tttgccactt gccgctgcct tgataaagtc catggcgggc
     5761 agcactttgc agaatttggt gggtggagac gacctgattg ccttcaacat ccacaaacca
     5821 gacgaacaaa gacaacccat ataaaatcag tacaaacaag gcagcgcctc cgaaaaaccc
     5881 gatccgtctc tgtgctttgg ttttaaaaaa gggccaaccc cgtcggctaa cgatctccag
     5941 agcaaaacca ttctctgcgg caattgcctg aaaggccggc aaggcactgt ttcttataca
     6001 catgcgcaaa ttttcgccct gccatttaac atcccaaata tacacccctc ggctaagagc
     6061 caggttgatc agctgttccg ggttttttcc tttaatacgc aaggtaatga cgccacccag
     6121 atgattaaag aatctgttta tcatcctgcc ctccttgatc agtcaacaaa ccggcaggtg
     6181 ctgatatctc ctgcgatttt tatttcgtcc ggcaacaacg ccttgatctc caaatgatca
     6241 cccagtattt ccagaaaccc ccggctcaga ttgatacgca ggcgttggtt gctgtactcg
     6301 atgatgccgc gatgattctc caaataaagt tctcctcggc caatcagtgt aattttgggg
     6361 atatccagta ccaaatcctt cggtatctcc agtaaatcag ccatcgcccg gctgatcatc
     6421 tctttgcttt tttccaatta aatgcctccc cggacaggct gaagcctgtt gcttttcaat
     6481 gcaaatatat gccacgagcc gtctttccat gctttctaaa ataaggtcca gtatgattgc
     6541 gcttggatct ttatgaaatt tagcttccgc tgatgtatgt aaatgaatcg agcccgcacc
     6601 acttcattat ggtacgggct caatttataa tattgctttt tgccaactgc tgtgaaggca
     6661 gtcacttagg ttcttatttt atccaatatc gagagaatcg agatccatct ccgccagcaa
     6721 ttgttttaat ctgacggctt cctccttgtt caaggtaacc cccttgccca ttctttcatg
     6781 ccccggtccc cagtcgcgta tatccagttt ggccttgcga tcattccagc tgaccagatt
     6841 gatctctttt tgccagcctt tgctgacatc ccccagagtc cccaattccc gggtaatgag
     6901 aaattttata tccgccattt atacagcctc ctttttgttt ttgccggcta tccgggtaca
     6961 caaccaataa tctcccgacc ggtctaaaac aattgttctt ttttacattt taaaccatta
     7021 atggtacatt gtcattcata aaattggtta cagtaaaacc gccccgggat aatgccctga
     7081 agcggtttat gatctgttta tttatgttga cactagaaaa ttgacaaggc tacaatgctg
     7141 accaaaaagc ttaccaaaac gatactgatt atgaacagca ccgccattct ctgcttttta
     7201 ttttgcatgc cccgattcat gaaggtcact agcgcacacc cccttagata atgaaaacct
     7261 ggcggcctga aaagctacca ggtttattgg tttccatgat gatctcactg acaagccggt
     7321 agatttcggt ttttgtcatc tgcaacgcca ggaaatacta gaattttttc tttttacgag
     7381 ccgcctcaga tttcttcttt ttccttacac tgggcttttc gtagtgttcg tgttttctaa
     7441 tatcctgtaa aactcctgac ttttggcagg agcgcttgaa tctcttcaat gcactgtcca
     7501 gggactcgtt ttttcctact tttacttcgc tcattatatc cctcccctca tatgaccagg
     7561 cta
//
LOCUS       DAOTHZ010000092         7232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_12328_length_7232_cov_6.210394, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000092 DAOTHZ010000000
VERSION     DAOTHZ010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12328_length_7232_cov_6.210394"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3384
                     /locus_tag="P5549_09115"
     CDS             <1..>3384
                     /locus_tag="P5549_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13271.1"
                     /translation="FKLSSDANGYLNGDILIGGPNATVGTALPDVTYTLAVEARNNYN
                     RNETVDEVNVVLTIDGTDQSFTSGQTLEGQSVNVSKITVKSASHSFWTYANDYKTVLD
                     TSSSTPRVKIIVYMQQAIGLVGEVKNKNTGQAVTDLRIRLENTNGNKPVGLSPIRNEN
                     GTFSNLYGLTRQFYILTIEADGYEPFRIKLDPSNAVDYTSGDAYAPKKAYLFTGDMAV
                     KLVPLTKPDILEETLQMDRFGAFIPGVKRSGKENTLNLVDNTAAEEALTMSWSLQVKP
                     PAEFAVTLPKFDQDGNSAGTEQIQIKDEVAEVWVIDRRSFADRTFKDKPTDVVLPSTD
                     AADYSPQKVLRWLQDFNEGTLEEVKYVYHTKVGAGGIGAVDSGTGNQIISGQIPLWTL
                     PPDDFRPTFVVVTKMGAAEVYNFDYSTGTIANLPPGLPDEHALVGLRIPDWFAGVLDT
                     AGYAATASTMFGDAAREQVQNRTPAGSIMPVGTCTAVIEVDDKNCLNYDYSLGVELVH
                     GSKTPSNGMLAAAPGMLGFSVFGSLQAKLAGMDKQFTIKSLAGLGASTVDASKLVPKA
                     FGKLGVTAEFDPMPQGAFEIIDDYVFDNANRPLEKAIIYAGRGEVGVKFTASGFQLLG
                     PIPKVGWVLLALKNSGALDAGAVMKGLVGVRGLQESTTVYPQNTEHYTISGGSLVQQA
                     VPLGADESSGTDTSAGGEAGSEVGSSEMAAPLGGFEPMEINEEEPRQLQRNFIGGQRD
                     SQVIPRSTKVAQLSEARKIELGLTDTDTALEICANFGLGLYVTTAAGNVGAEASIEVA
                     GENSWTSKPSLMATINPKRDAPIITEVSGDVRATADLYARLWIARFQKNYVWASIPIE
                     YQFTTEPSFSLIPMQITEHSVGRGDYDVSVFYGTPVLLIDDFLVIGDYSDDSAGSVAF
                     TYADMATKGGNMRLMASQGGSSNGTQGWQTPVAIASTDGAVISHDIVSLPEGAGYMAA
                     WTEIAATDLEKTTPPAVLKYSQGDSNFTNWSTPQVMANLDEVPTNLELLQKGNDVAAI
                     YLATDEGPASVNRTIYGSIWDGSEQAWNTPAQIAAKNEVKGYAACVAGNSAEQVLLVY
                     VNGLNELQSLQWNGSSVSGAVKLADGAGNDLVLVSQDGG"
     assembly_gap    3385..3484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3485..4240
                     /locus_tag="P5549_09120"
     CDS             <3485..4240
                     /locus_tag="P5549_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13272.1"
                     /translation="KLADGAGNDLVLVSQDGGEATLLWSGLNRGIGMFSYTGSGFLDR
                     GLPLSQATPNELAAQRMGTDKLLVVWTGGDGEGLMSATLGMDGSIVQTPALTVAAETG
                     RFFSPEIVSTTDAVSGETSLYTLALHENDDVELRGYKYGANSVDECFIATAAFGSKLE
                     PAVVLLRQFRDRFLLTNAIGRAFVDFYYRHSPPLANLIAKNEALRTMTRGLLLLPIAI
                     AWSLLHPFASALLLLALLGMAAFTRRVGRRLQS"
     gene            complement(4348..6282)
                     /locus_tag="P5549_09125"
     CDS             complement(4348..6282)
                     /locus_tag="P5549_09125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW13273.1"
                     /translation="MSYKELRQRWEELHKNPALRNSLRPEIRDSWERSCDFGIDPYLR
                     ENPYICTAAELEQYRQASAYLLEASRPVMNSLQEYVAGSGFVIVLADANLCLLEVIGD
                     QQALEWARHARAIEGSLWREELVGTNTASIVMSLVKPYYLYGFEHFCLFSSVASSFCS
                     PIIDEGKLIGGLGMIAPHDLGNNQTLGMAVAATMHIKSKIEMKKIENLHEVINDSMTE
                     GLLALDANRSITLMNENCARILKVDHKSSVIGRSIYELLANHPENHHFINSITQGRTI
                     TDENFDLLVKNESIPCNITCNPFKSLNALNGGVVIVIRERQRINRLVRNSIGGGATMY
                     FSDIVVKNQRFAETLKTAQAAASSTSNVLLLGESGTGKDIIAQAMHNASPRRTNPYLA
                     INCAALPRDLIASELFGYEEGAFTGARKGGNIGKFELADQGTIFLDEIGDMPLYLQAT
                     LLRVLEEKKVMRLGSSKLIPVDVRIIAATNKDLEVEIEHNRFRRDLFYRLGVIKIDLP
                     PLRDRPEDILELTRVFVEKTCSKFNKPVLKVAEEVLNAFTAYDWPGNVRELQNLIEAA
                     VQLCPNEIITYDLIEKISAVVVNNQTRSTPFQADESMEKQMIMEALARHKFKKKETAE
                     ALEMSRSTLYRRMKELEIRG"
     gene            6668..>7232
                     /locus_tag="P5549_09130"
     CDS             6668..>7232
                     /locus_tag="P5549_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRW13274.1"
                     /translation="MRTSQEYLQKLAGMRPNIYLDGECIGRDYPKVVHASYAIQLTFD
                     KASDPEYSKWLTTTSHLSGQPINRFNHIHQSPEDLLLKQEMTRKMCNLVGGCIQRCMG
                     ADSMNGLSVVTKNCDLKYGTDYHARWLKYLAYFQENDLVAAASQTDAKGDRSKRPSEQ
                     DDPDMYLHVVERRDDGVIVSGAKLHNTM"
BASE COUNT         1635 a         1831 c         1992 g         1674 t
ORIGIN      
        1 gttttaaact gagcagcgat gccaacggtt atctgaacgg cgatatttta atcggcggcc
       61 cgaacgccac tgtgggaaca gcgttgccgg atgtcaccta cacgctggcg gtggaggccc
      121 gcaacaacta taaccgcaat gagacggtgg acgaagtaaa tgttgtgcta accattgacg
      181 gaacggatca gagctttacc tccgggcaaa ccctggaggg acagtccgtc aatgtcagca
      241 aaataacggt caagagtgcc agccattcct tctggacata cgccaatgac tataaaacgg
      301 tgctggatac gtcctcctcc acgccccggg tcaagatcat cgtttacatg cagcaggcca
      361 ttggtctggt gggcgaggtc aagaacaaaa acaccggcca ggcggtcacg gatttgcgga
      421 ttcgtctgga aaataccaac ggcaacaagc cggtgggttt gtccccgatc cgcaacgaaa
      481 acggtacgtt ctccaatctg tatggactga cccgtcagtt ctatatcctg accatcgagg
      541 cagacggcta tgaacctttt cgcataaaac tggacccgtc caacgcggtc gactatacct
      601 cgggtgatgc ctatgcgccc aagaaggctt atctctttac cggcgacatg gcggtcaagc
      661 tggtgccgct gaccaagccg gacattctgg aggaaacgct gcagatggat cgtttcgggg
      721 cctttatccc cggggtgaaa cgcagcggca aggaaaatac gctgaatttg gtggacaata
      781 cggcagcgga agaagcgctg accatgagct ggagcctgca ggtcaagccg ccggctgaat
      841 ttgcggtgac gttgcccaaa tttgaccagg atggcaacag cgccggcacc gagcaaatcc
      901 agatcaagga tgaggtggcg gaggtctggg tgatcgaccg gcgttccttt gcggaccgaa
      961 cttttaaaga caagcccacc gatgtggtgc tgccttcgac ggatgcagcc gactactcac
     1021 cgcagaaggt gttgcgctgg ctgcaggatt tcaatgaggg tacgctggaa gaggtcaaat
     1081 atgtttacca taccaaagtt ggagcgggcg ggatcggcgc cgtcgattcc ggtaccggca
     1141 accagatcat ttccgggcag ataccgctct ggactttgcc gccggatgat ttccggccga
     1201 ccttcgtggt ggtgaccaag atgggcgcgg cggaggttta caattttgac tacagcaccg
     1261 gcacgatcgc caatctgccg cccgggcttc ccgacgagca tgcgctggtg gggttacgga
     1321 tcccggattg gtttgccggg gtgttggata cagccggtta tgcggcaact gcctcgacca
     1381 tgttcggcga tgccgcccgt gagcaggtgc agaaccgtac gcctgccggc agcatcatgc
     1441 cggtggggac gtgtaccgca gtgatcgagg tggatgacaa gaactgtctc aattacgact
     1501 actcgctggg agtcgagttg gtgcacggtt cgaagacgcc ttccaacggc atgctggcag
     1561 cggcgccggg gatgctgggc ttcagtgttt ttggttcgct gcaggccaag ctggccggga
     1621 tggataaaca gtttacgatc aaatccttag ccggcctggg agcatcaacg gtggacgcca
     1681 gcaagctggt gcccaaggcc tttggcaaac tgggtgtcac cgcggaattt gatcccatgc
     1741 cacagggggc gtttgagatt atcgacgatt atgtctttga caatgccaat cgcccgctgg
     1801 agaaggcaat catctatgcc ggccggggcg aggtgggtgt gaagtttacc gccagcgggt
     1861 ttcaactgct ggggccgatc cccaaggtgg gttgggtgct gctggcgctg aaaaactccg
     1921 gagcgctgga tgcaggcgcg gtaatgaaag ggttggtcgg cgttcggggg ttgcaggagt
     1981 cgaccacggt ttatccgcag aatacggagc attatacgat ttcaggcgga agtttggtgc
     2041 agcaggcagt gcctttgggg gcagatgaat cttccggtac agatacgagt gcaggcggtg
     2101 aggcgggctc ggaggttgga tcgtcagaga tggctgcgcc tttgggtggc ttcgaaccta
     2161 tggaaataaa tgaagaggag ccgcggcagt tgcagcgcaa ttttatcggc gggcagcgcg
     2221 acagtcaggt gatccccagg agtaccaagg tggcccagtt gagtgaagcc aggaagatcg
     2281 aactggggct gaccgacacc gatacggcgc tggagatctg cgcaaatttc ggcctgggtc
     2341 tgtatgtaac gactgctgcc ggcaatgttg gggcggaagc ttccattgaa gtcgccgggg
     2401 agaacagttg gaccagcaaa ccgtcgctca tggcaaccat caatcccaag cgcgacgcgc
     2461 cgatcattac ggaggtttcg ggggatgtgc gggctacggc ggatctgtat gcgcggctgt
     2521 ggattgccag gttccagaag aattatgtgt gggcctcgat cccgattgaa tatcagttta
     2581 ccaccgagcc gagttttagt ttgatcccca tgcagatcac ggagcacagc gtgggccgcg
     2641 gtgattatga tgtttcggtg ttttacggca caccggtgct gctcatcgat gatttcctgg
     2701 tgattggcga ttattccgac gacagcgccg gcagcgtagc ctttacctat gccgacatgg
     2761 caaccaaggg cggcaacatg cggctcatgg ccagtcaagg gggcagcagc aacgggacgc
     2821 agggctggca aacgccggtg gcgattgcgt ccaccgatgg cgcagtaatc agccatgaca
     2881 ttgtcagcct gccggagggc gcaggatata tggcagcctg gacggaaatc gcggcgacgg
     2941 atttggagaa gacgaccccg ccggcggtac ttaaatattc gcagggcgac agtaatttta
     3001 ccaactggag cactccgcag gtgatggcca atttggacga ggtgccgacc aaccttgaac
     3061 tgctgcaaaa aggcaatgac gtggcggcaa tctatctggc aacggatgaa ggaccggcat
     3121 cggtgaaccg caccatttac gggtcgattt gggacggctc ggaacaggcg tggaacacgc
     3181 cagcgcagat tgcagccaaa aacgaggtca agggttatgc ggcctgcgtc gcgggaaatt
     3241 ctgccgaaca agtgctgctg gtctatgtca acggtttaaa tgaactgcag tcgctgcaat
     3301 ggaacggcag cagcgtatcc ggggcagtta aacttgccga tggggccggc aatgatctgg
     3361 tcttggtcag ccaggacggc ggcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnaaactt gccgatgggg ccggcaatga tctggtcttg gtcagccagg acggcggcga
     3541 ggccacgctg ctgtggagcg gccttaaccg cggcatcggc atgttcagct atacaggcag
     3601 cggttttctc gatcgcgggc tccccttgtc gcaggcaacg ccaaatgaac tggccgcgca
     3661 gcgcatgggt acagacaaat tactggtggt gtggaccggg ggggacggcg agggtctgat
     3721 gtcggcaacg ctaggcatgg acggcagtat agttcagaca ccggcgctga cagtcgctgc
     3781 ggagacgggt cgtttcttca gtccggaaat cgtcagcacc actgatgcag tcagcggcga
     3841 aacgagtttg tatacgctgg cgctgcatga aaatgacgat gtggagctgc ggggctataa
     3901 atatggcgca aacagtgtgg atgaatgttt tatcgccaca gcggcttttg gttcaaaact
     3961 tgagccggcg gtggtgctgc tgcggcagtt ccgggatcgt ttcctgctga ccaacgcaat
     4021 cgggcgagcc tttgtcgatt tctactatcg ccactccccg cctctggcca atttgattgc
     4081 gaaaaatgag gcgctgcgga ccatgacccg gggactgctg ctcctgccca ttgcgattgc
     4141 ctggtcattg ctgcacccct tcgcgtcggc attgttgctg ctggccctgc tgggtatggc
     4201 agccttcacc cgccgtgtcg gaaggcggct tcaatcatag ccgcattttt cctgttgcca
     4261 taaggcttgg ttttcatatg cctccccaac ggaggcatat ttttttatcc aaacagtcgg
     4321 ctgtattatt cacctttgac atgcgcttta tccacgtatc tccaattcct tcatcctgcg
     4381 atacagcgtg cttcttgaca tctccagcgc ctctgcggtt tcttttttct tgaatttgtg
     4441 ccgggctaat gcttccataa tcatttgttt ttccatcgac tcgtctgcct gaaacggtgt
     4501 actcctggtt tgattgttca ccaccacggc agatattttt tcgatcaaat catatgttat
     4561 tatttcattc gggcacaatt gtacggcagc ttcaatcagg ttttgcaatt cccttacatt
     4621 gccgggccag tcataggcag tgaaggcatt cagtacttct tcagcaacct ttaaaaccgg
     4681 cttgttgaat ttgctgcagg ttttctccac aaatacccgg gtcagttcca ggatatcttc
     4741 aggtctgtcc ctgagcggcg gcaggtcgat tttaatcaca cccaggcgat agaacaagtc
     4801 ccgacggaag cggttatgct cgatttcaac ttccaaatcc ttgttggtag ccgcgatgat
     4861 gcgcacatcg accgggatca gtttggaact gcccagccgc atgactttct tttcttccaa
     4921 aacccgcaac agggtagctt gcagataaag cggcatatct cctatttcat ccagaaatat
     4981 ggtgccctga tcggccaatt caaatttgcc gatgttgccg ccctttcttg cgccggtaaa
     5041 ggcaccttct tcatagccaa acagttcgct ggcaatcaaa tcgcggggca gagcggcaca
     5101 attaatggcc agatagggat tggtgcgacg cgggctggcg ttatgcatgg cctgggcgat
     5161 aatgtctttg ccggttccgc tttcccccag cagcaatacg ttggaggtgg aggaagccgc
     5221 tgcctgggcg gtttttaaag tctcggcaaa acgctggttt tttactacaa tatcactgaa
     5281 gtacatggtt gctcccccgc cgatggaatt gcgaaccagc cgattgatgc gctgccgctc
     5341 ccgaatcacg atcactactc cgccattcaa ggcattgaga cttttaaagg gattgcaggt
     5401 gatgttgcag gggatgcttt catttttcac caacaaatca aaattctcat ccgtaatcgt
     5461 ccgtccctgg gtaatggaat taatgaaatg atggttttcc ggatgattag ccagcagttc
     5521 ataaatgctt cggccgatga ccgatgattt gtgatcaact ttgaggatcc gggcgcagtt
     5581 ttcgttcatc aatgtgatgg aacgattggc atccagcgcc agcaggcctt ctgtcatgga
     5641 atcattgatg acttcatgca ggttttcaat ttttttcatt tcaattttgg atttaatatg
     5701 catggtcgct gccacggcca tccccagcgt ctgattattc cccagatcat ggggagcaat
     5761 cattcccaac ccgccgatta atttgccctc atcaatgatg ggggaacaaa agctgctggc
     5821 aacgctggaa aacaggcaaa aatgctcaaa accatacaaa tagtagggtt tgaccagcga
     5881 catcacaatc gatgcggtat tggttccgac cagttcttcc cgccacaaag atccctcgat
     5941 tgcgcgggca tggcgagccc attcaagggc ctgctgatcg ccgatgactt ccagcaaaca
     6001 caggttggca tcagccaata cgatgacaaa acctgatccg gccacgtatt cctgcaaact
     6061 gttcataacc gggcgggaag cttccagcag gtaggcagat gcctgccggt attgctccag
     6121 ctctgccgca gtacatatat aggggttttc ccgcaaatac ggatcgatgc caaaatcaca
     6181 gcttctttcc caggagtccc ttatttcagg acgtagactg ttgcgcaggg cagggttttt
     6241 atgcagctct tcccatcttt ggcggagttc tttataagac acgggtcatt cctcccagtc
     6301 attcatctat tcattgcata ataccacaac cgaatacaat gtgacacata cagtttcacc
     6361 tgttcttttg cacccctgcc atttatcttt tcgttcatcc ttttgaaaca tttgaaacgt
     6421 ttttgcatcc ttttgttgca gaagacgttt cacgccccgt tgcaaacttc tttcttttta
     6481 ataaaaaaag cggttttatc ggcacttaca tccgtttcat cttctggcac aatgcttgcg
     6541 tatatgggta gctatcagtt cctcttgaca gtttcagtta ccttacggaa aggagcgata
     6601 aaagactgcc aacaatcaat tccagccatc gatccccaat atataatata aaaggagatg
     6661 ttgataaatg agaacctcac aggaatatct gcaaaaactg gccggtatgc ggcccaacat
     6721 ttatctggac ggtgagtgta ttggccgtga ctatcccaaa gttgtccatg ccagctacgc
     6781 catccagctt acttttgaca aagccagtga tcccgagtac agcaaatggc tgaccaccac
     6841 atctcatctc tccggtcagc ccattaaccg ttttaatcat atccatcaat caccggaaga
     6901 tttattgctg aagcaagaga tgacccgtaa aatgtgcaac ctggtcggcg gctgtatcca
     6961 gcgctgcatg ggtgcagatt ccatgaatgg cttgtcggtg gtcacgaaaa actgcgacct
     7021 caaatatggc actgattatc atgccagatg gctcaagtat ctggcctact tccaggaaaa
     7081 cgacctggtc gcagcggctt cccagacgga tgccaaaggg gatcgcagca aacgccccag
     7141 cgaacaggat gatccggata tgtatctgca tgtagtagaa agaagagatg acggggtaat
     7201 tgtttccggc gccaaactgc acaacaccat gg
//
LOCUS       DAOTHZ010000093         7162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_12470_length_7162_cov_8.487829, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000093 DAOTHZ010000000
VERSION     DAOTHZ010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12470_length_7162_cov_8.487829"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..282
                     /gene="rpsP"
                     /locus_tag="P5549_09135"
     CDS             16..282
                     /gene="rpsP"
                     /locus_tag="P5549_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRW13275.1"
                     /translation="MATKIRLKRMGAKKSPFYRMIVADARSPRDGRFIEEIGYYDPST
                     DPATIKIDEEKALKWLSTGAKPSDTAKSLLQKQGIMSKFAEGRK"
     gene            313..543
                     /locus_tag="P5549_09140"
     CDS             313..543
                     /locus_tag="P5549_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRW13276.1"
                     /translation="MKELVEYIARALVDNPDQVQVIEVDGEKSNLIELRVAPEDMGKV
                     IGKQGKIAQSIRTLTKATAAKEGKRVTVEIMR"
     gene            779..1294
                     /gene="rimM"
                     /locus_tag="P5549_09145"
     CDS             779..1294
                     /gene="rimM"
                     /locus_tag="P5549_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640897.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRW13277.1"
                     /translation="MDANELVSIGRIVGTHGYKGMLKVMPLTDFPERFQGLAEIRVVQ
                     GRKISRHRIASCSPYKDLLLMKLEGIDDLETAQTYRQAWLGVEEKDVFPLPPGYYYHF
                     QLVGLAVYDIEKGYLGILKEVLETGANDVYAVDSEVYGEILLPAIQQVILEINLEEKK
                     MTVKLLDGLLD"
     gene            1343..2089
                     /gene="trmD"
                     /locus_tag="P5549_09150"
     CDS             1343..2089
                     /gene="trmD"
                     /locus_tag="P5549_09150"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRW13278.1"
                     /translation="MRIDILTLFPEMFASPLQASMIGRARQNGLLQINTVNIRDFALD
                     KHQQVDDYPYGGGAGMVMKVDVLARAIQHVKDQPGRVVYLSPQGQRLDQTIVTRLAGE
                     DHLILLCGHYEGIDERAMTYIDEEISIGDYVLTGGELGAMVVVDAVSRLVPGVLGDDA
                     SAEDESFAEGLLEYPQFTRPRSFAEMEVPPVLLSGHHEEIRRWRKKLSLERTLLKRPD
                     LLLNRAFDAEEQSLLEEILFVRKKEKGAKR"
     gene            2076..2654
                     /locus_tag="P5549_09155"
     CDS             2076..2654
                     /locus_tag="P5549_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRW13279.1"
                     /translation="MRNAKVYIALLHHPMYNKRMDIVTTSVTNLDLHDIARAARTYKA
                     DGFYVVHPAPNQQQLIGDILSYWQEGYGGSYNPDRREAFAILHRKEDLAQVVAEIEAQ
                     TGSRVVTVATDARRYSQTVSYRWLKEKIFSEDQVFLLLFGTGWGMEQTLMEKCDYILE
                     PIAPDSDYNHLSVRSAVSIIIDRLLGEFWFNL"
     gene            2765..3115
                     /gene="rplS"
                     /locus_tag="P5549_09160"
     CDS             2765..3115
                     /gene="rplS"
                     /locus_tag="P5549_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRW13280.1"
                     /translation="MQDIIKSIEEEQYKKDLPRFGPGDTVKVHVKVVEGTRERIQLFE
                     GTVMKIRGTGLSQTFTVRRISYNVAVERTFLMHSPKIAKIEVTRKGKVRRARLFYLRG
                     LTGKKARVKEKSRF"
     gene            3200..4036
                     /gene="ylqF"
                     /locus_tag="P5549_09165"
     CDS             3200..4036
                     /gene="ylqF"
                     /locus_tag="P5549_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HRW13281.1"
                     /translation="MAINWYPGHMVKAKREIAENIKLVDIVLMLLDARAPFSCRNPDL
                     EKIISRKKIIFILNKMDLASSASIQAYQQKLQQEGYPAAAMDSSAGKGSKEVLQLIKS
                     VYQEQAEEMLSKGRRIRSPRVMVVGVPNIGKSTFLNCLVGKKVAQTGAKPGVTRGKQW
                     VRVREDIEFMDTPGLMWPKVEDEEQGLKLALLNIVGENAYQEYDVALYLLRTLQAKTP
                     QIFYTRYKVENVQLEAEEILAEIARNRGYLNKGGTPDVDKTCHLLLQDYRRGNLGRIS
                     LD"
     gene            4260..5423
                     /gene="yedE"
                     /locus_tag="P5549_09170"
     CDS             4260..5423
                     /gene="yedE"
                     /locus_tag="P5549_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HRW13282.1"
                     /translation="MRKNNYIYNYKLFIRGIIKLDKGKWFILVTGLIIGAIAVVLVKL
                     GNPPNMGFCIACFERDIAGALGLHRAGVVQYLRPEIIGIVLGTMLTSLFAGEFRSRGG
                     SSTLVRFLLGMFMMIGALVFLGCPLRDVLRMAGGDYNAFVGLIGFIGGVGGGVWFLRR
                     GFFLGRAEESHSKLGGFILPAIFAILLFLLLKGTVFNPDAGGPLFFSKEGPGSMAAPL
                     LISLAAGLLVGFLAQRTRLCMSGGIRDFILMRDNHLLLGFIGIFLGALVLNIALGFFK
                     TGFSGQPIAHSLHVWNFLGLFLVGLTATLLGGCPLRQLIMSGEGDTDAAAVVLGMIIG
                     AAVCHNFMLAAAAAKAAAPGVAAVAGGPGIYGQIACVAGIVVTAIIGWSFREE"
     gene            5435..5644
                     /locus_tag="P5549_09175"
     CDS             5435..5644
                     /locus_tag="P5549_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRW13283.1"
                     /translation="MEKVDVRGMSCPLPVIKTKKIMDQGANEILVTGSTAVSLENVSR
                     LAKTRNYNINVKKADKNDWEIELVK"
     gene            complement(5783..7132)
                     /locus_tag="P5549_09180"
     CDS             complement(5783..7132)
                     /locus_tag="P5549_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13284.1"
                     /translation="MSNDRRVYQNKLVSANHAVKIVQSGDWVSYGSLNAQACSLDKAL
                     AKRKDELKDVKVWTLLCLRMPEIVKVDPQGESFCCQSWHFSGTDRKMAKQGTPIYYTP
                     MRYSELPRYIRENVAPIQVAMLGTGPMDKNGYFNFGPQNSHHKALCEKADKVIVEINT
                     NMPRCLGGYHEAIHISEVDLIVEGENEALVAPAPTPPTEIDQRVARRIVAEMEDGACI
                     QLGIGGMANAVGQMICDSDLKDLGCHTEMLCDAYVDLFAAGKMTGRRKNLNPEKMVYG
                     FALGSQRLFDFIDDNPRCASFPVDYTNRVAIASANDHLMSINNAVEVDIYGQICSESS
                     GTRHISGTGGQLDFVLAAYESRGGKSFVCLPSTYQKPDGKLASCIVPTLKTGAIVTDA
                     RTVAHYIVTEHGIFNSKGKTTWQKAEGIISLAHPDFRDDLIRAAQEQKLWSRYNKQF"
BASE COUNT         1787 a         1645 c         1981 g         1749 t
ORIGIN      
        1 caaggaggtg acaatatggc aacaaaaatc cgcttaaaga gaatgggagc caagaaaagc
       61 cctttctaca gaatgatcgt cgctgatgca agatcgcctc gtgacggacg ttttattgaa
      121 gaaatcggat attatgatcc ttcaaccgat ccggccacga ttaagatcga tgaggaaaaa
      181 gccctgaaat ggctttctac cggtgctaaa ccttcagata cagcaaaatc acttttgcaa
      241 aaacagggta ttatgagcaa atttgcggaa ggccgcaagt agggtcgcac gacccagggg
      301 ggtgaatcac aagtgaaaga gctggttgaa tatattgccc gcgccctggt ggataatccc
      361 gaccaggttc aggtgatcga agtggacggc gaaaaatcca acctgattga actgcgggta
      421 gcgcctgaag atatgggcaa ggtgatcggc aaacaaggca aaattgctca gtcgattcgt
      481 accttgacga aagctaccgc agccaaggaa ggcaagagag tcaccgtgga aattatgaga
      541 tagggcggca accatcgccc aagaattttt ccagggagaa tcgcctggtg aaccaggcca
      601 gcccgtctca tccgccgtac gtgttggggg acaatcactg ttcgggtgtg tccccatccc
      661 cgtgcgtcgt cttgggcacg ggcttttcag ttgttcactg cttctccttg aagggatgtc
      721 cggacagaag ttcgacgttt caccacactt ttacccacca tgttgggaga tggatgtaat
      781 ggatgcaaat gaattagtga gcatcggcag aatcgtaggt acccatggct acaaagggat
      841 gttgaaagtc atgcccctga ccgattttcc ggagcgtttc cagggtctgg cagagattcg
      901 ggtggtacag ggacgtaaaa tttcccggca cagaattgca tcctgcagtc cttacaaaga
      961 cctgctgctg atgaaactgg aaggcatcga cgatctggaa actgcccaaa cctatcgaca
     1021 ggcctggctg ggtgtcgagg agaaagacgt atttccgctg ccacccggct attattatca
     1081 ttttcaactg gtgggcctgg cggtttatga tatcgaaaaa ggatatctgg gaattctcaa
     1141 ggaagttctg gaaaccggag ccaacgatgt ttatgcggtc gattccgagg tttatggaga
     1201 gattttgctg ccagcgattc agcaagtaat ccttgagatc aacctggaag aaaaaaagat
     1261 gacggtcaaa ttgctggacg gattgctgga ctagccctgg tccgcttttt ggatgcaatc
     1321 aaccgtgatt tgggagaata ccatgcgcat cgatatttta acgcttttcc cggagatgtt
     1381 tgcttcgccg ctgcaagcca gcatgatcgg ccgcgccaga caaaacgggc tcctgcagat
     1441 caataccgta aacatcaggg actttgccct ggacaagcac caacaagtag atgattatcc
     1501 ttatggcggc ggtgcaggga tggtcatgaa agtggatgta ctggcccggg ctattcaaca
     1561 tgtcaaagat cagcccggca gggtggttta cctgtccccg cagggacagc gcctcgacca
     1621 gaccattgtg acccggctgg ccggagaaga ccatctgatc ctgctctgcg gccattatga
     1681 aggcatcgat gaacgggcca tgacctatat tgatgaagaa atatccatcg gcgattatgt
     1741 tctgaccggc ggtgaactgg gtgccatggt ggtggttgat gcagtctccc ggcttgttcc
     1801 cggggtgctg ggtgatgatg cctcagctga ggatgaatcg tttgcggaag gtttattgga
     1861 gtatccccag tttactcgtc cgcgctcttt tgcagagatg gaagtccctc cggtgctgct
     1921 ttccggacac catgaagaga tccgccgctg gcgcaagaaa ctgagtctgg agcggacctt
     1981 gttgaagaga cccgatctgc ttttaaaccg cgcttttgat gctgaagagc agtccttgct
     2041 ggaagaaata ttgttcgtca ggaagaagga gaaaggtgcg aaacgctaaa gtctatattg
     2101 ccttgctgca ccatcccatg tacaacaaac gcatggatat cgtaaccacc tcggttacca
     2161 atctcgattt gcatgatatc gcccgggctg cccgcaccta caaggcagat ggattttatg
     2221 tcgtccaccc ggcgcccaat caacaacagc tgattggcga cattctgtcc tattggcagg
     2281 aaggctacgg aggcagctac aatcctgacc gtcgggaagc ttttgccatc ctgcaccgga
     2341 aggaggatct tgcccaggtc gtagccgaaa tcgaggcaca aaccggaagc agggtggtga
     2401 ccgtcgctac cgatgcgcgc cgttattccc agacggtttc ctatcgctgg ctgaaagaaa
     2461 agatattcag cgaagaccag gtctttctcc tgctttttgg tactggttgg ggcatggaac
     2521 agaccctgat ggaaaaatgc gactatattc tggaaccgat tgcgccggac agtgattaca
     2581 atcacttgtc agtgcgcagc gcggtttcca tcattatcga tcgcttgctg ggagaatttt
     2641 ggtttaatct gtagttctcc cttgtagtca ataaaatact gtgttaaaat aggcatgttc
     2701 tgtattaaag gtggtccgct gcatgacagc catgtaagaa cgcctgtgga aggaggtcga
     2761 aatcgtgcaa gacatcatta aatccattga agaagagcag tacaaaaagg atctgcctcg
     2821 ctttgggcct ggcgataccg taaaagtaca tgttaaagtt gttgaaggta cacgggaaag
     2881 aattcagctt ttcgagggta ccgtcatgaa aatcagaggc acgggtctgt cacagacgtt
     2941 caccgtacgg aggatttctt acaatgtggc cgtggagaga acattcctga tgcattctcc
     3001 caaaatcgcc aaaatcgaag tcactcgcaa aggcaaagtg cgtcgcgcca gactgttcta
     3061 tctgcggggt ctgaccggca agaaagcccg ggtcaaggaa aagagcagat tctaagcaaa
     3121 gacttaaatg attgggaagg agaataccgt gcggtattct ctttttttct ctcgccaggt
     3181 tggcaaagga ggcgattaca tggcgattaa ttggtatccc gggcatatgg tcaaagccaa
     3241 acgagaaata gctgaaaaca tcaaactggt cgacattgtg ctgatgctgc tggatgcgcg
     3301 cgctccgttt tcatgccgca accctgacct ggaaaagata atcagccgca aaaagattat
     3361 ttttatcctc aacaaaatgg atctggcctc gtctgcgtcc attcaggcgt atcagcaaaa
     3421 actgcagcag gaaggctatc ccgctgcggc catggattca agcgccggca agggaagcaa
     3481 ggaagtgctg cagctgatta aaagcgttta tcaggagcag gccgaggaaa tgctcagcaa
     3541 aggccgacgg atcagaagtc cgcgggtgat ggtggtaggg gttcccaata tcggtaaatc
     3601 gacattttta aactgcctgg tgggtaaaaa agttgcccag accggcgcca agccgggcgt
     3661 gacccgcggc aaacaatggg tgcgggtacg cgaggacatt gagttcatgg atacccccgg
     3721 tctgatgtgg cccaaggtcg aagatgaaga acagggccta aaactggcct tgctgaatat
     3781 tgtgggggaa aatgcctacc aggaatacga tgtagccctt tacctgctgc gcacgctgca
     3841 ggccaaaacg cctcaaattt tttatactcg ctacaaagtg gaaaatgttc agttggaggc
     3901 ggaagaaata ctggccgaga tcgcccgtaa ccgcggttat ctgaacaaag gcggaacgcc
     3961 tgacgtcgac aagacctgcc atttgctgct gcaggattac cgccgcggca atctggggcg
     4021 gatcagtttg gattgacagg aagcaagggg acggttcaag ccattgttgg ctctgtctgg
     4081 cagaagcaga atcgtcccgc gtttgcactt tccggttgac ggaaaagcgc atgagcagca
     4141 caaaacgttt aatatttata ataatatgta ttatacctgc ttattgataa gcgggttttt
     4201 ttattgaaaa aatttttata caaagaagga taaatatcga ttatgtagaa tattataaaa
     4261 ttagaaaaaa taattatata tataactata agttatttat aagggggatt attaagttgg
     4321 acaaaggcaa atggtttatt ctcgtcacgg gacttatcat tggagcgatt gcggtggtgc
     4381 tggtcaagtt gggcaacccg cccaatatgg gattttgtat tgcctgtttc gaaagagaca
     4441 ttgccggcgc gctgggattg caccgggccg gggtagtgca gtacttgcgt cctgaaatta
     4501 tcggcatcgt gttgggcacc atgctgactt ctctgtttgc gggtgagttc aggtcccgcg
     4561 gaggttcatc tacattggta cgatttctcc tggggatgtt tatgatgatt ggcgctttgg
     4621 tatttctggg ctgtccgctg cgcgatgtgc tgcgcatggc cggtggtgac tacaatgcat
     4681 tcgtcggact gattggcttt attggcggcg tcggcggagg cgtatggttt ttgcgtcggg
     4741 gattctttct gggtcgggcg gaggaaagcc acagcaagct gggcggcttt atcctgccag
     4801 cgatctttgc catcctgctg tttctcttgc ttaaaggaac cgtatttaat cccgatgcgg
     4861 gcggtcctct gtttttcagc aaggaaggcc ccggttccat ggccgccccg ctgcttatct
     4921 cgctggctgc cggtctgttg gtgggatttc tggcgcagcg cacccggttg tgcatgagcg
     4981 gcggcatcag ggatttcatc ctgatgcggg acaatcattt attactaggc tttatcggca
     5041 tcttcctggg ggctttggtt ttaaacattg ccttgggatt tttcaagact ggttttagcg
     5101 ggcaaccgat tgctcacagc ctgcatgtat ggaacttcct ggggctgttc ctggtaggcc
     5161 tgacggctac actgctgggt ggatgcccct tgcgacaact gatcatgtcc ggtgaaggcg
     5221 atacagatgc tgccgcagtg gttttgggaa tgatcatcgg agcggctgta tgtcacaact
     5281 ttatgctggc tgctgccgcg gccaaggcgg ctgcccccgg cgtggctgca gttgccggag
     5341 gaccgggtat ttacggacaa attgcttgtg tggcaggaat tgttgtaacg gccatcatcg
     5401 ggtggtcttt ccgagaagaa taggaggatt ggcaatggaa aaagttgacg tacgaggcat
     5461 gagttgccct ttgccggtga ttaaaacgaa aaaaataatg gatcaagggg caaatgaaat
     5521 cctggtgaca ggctccactg cggtatcact ggaaaacgtt agcagactgg ctaaaacccg
     5581 gaattataac atcaatgtga aaaaagcaga taaaaatgat tgggagatcg aactggttaa
     5641 ataaaagtgg aatcaggcag agacaggctg aaaaaaagcg gttataaaac tgattctgga
     5701 aaatcgagcc cgtaccataa aacaggtacg ggctcgatta ttgtatgctt gtagctttca
     5761 ttatgaatat gggaataggc gattaaaatt gtttgttata tctggaccag agcttttgct
     5821 cctgggcagc gcggatcaga tcatcgcgga aatcggggtg ggccagggag ataatccctt
     5881 ccgccttttg ccaggtggtc ttgcctttgc tattgaagat tccgtgctcg gttacgatat
     5941 aatgagcgac ggttcgggca tcggtcacga tcgcgccggt ttttaaggtt ggcacgatgc
     6001 aggaggccag ttttccgtcc ggtttttgat aagtggaagg caggcagacg aatgatttgc
     6061 cgccccgtga ttcgtaagcc gccagcacga agtcgagttg tccgccggtg ccgctgatat
     6121 gccgggtgcc cgaggattct gagcagatct gcccgtaaat atcgacctcg acagcattgt
     6181 tgatggacat caggtgatcg ttggcggaag cgattgccac ccggttggta taatcaaccg
     6241 gaaacgaagc acaacgggga ttgtcatcga taaagtcaaa aagccgctgg ctgcccaaag
     6301 caaagccgta gaccattttt tcaggattaa gatttttgcg ccgaccggtc atcttgcctg
     6361 cagcaaacag gtcaacatag gcatcacaaa gcatttctgt atggcaaccc agatctttta
     6421 agtcagagtc acagatcatc tgccctacgg cattggccat accgccgatc cccaactgga
     6481 tacaggcccc gtcttccatt tcggcgacga tccgccgggc gaccctttga tcaatctcag
     6541 tgggcggggt gggggcgggc gcgaccaggg cttcgttttc gccttctacg atcaagtcaa
     6601 cctcggagat gtggatagcc tcatggtatc cgcccagaca gcgcggcatg ttggtattga
     6661 tctctacgat cactttgtca gctttttcac agagggcctt gtgatgagaa ttctgcggtc
     6721 caaaattaaa gtagccgttc ttgtccatgg ggccagtacc caacatggca acctggatgg
     6781 gcgcaacatt ttcccggata tagcgaggga gttcggaata gcgcatggga gtgtagtaaa
     6841 tcggggttcc ctgcttggcc attttgcgat ccgtcccgga aaaatgccag gactgacagc
     6901 aaaaggattc cccctgggga tcgaccttga cgatctcggg catgcgcaga cacagcaacg
     6961 tccagacctt tacgtccttc agttcgtcct ttcttttagc cagcgctttg tccagactac
     7021 aggcttgggc attgagggaa ccataactca cccagtctcc ggattgcacg atttttactg
     7081 catggttagc cgagacgagt ttgttttgat acactctccg atcattgctc aagtcaactt
     7141 cctccttttg acaggaaaaa tt
//
LOCUS       DAOTHZ010000094         6943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_12958_length_6943_cov_8.492160, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000094 DAOTHZ010000000
VERSION     DAOTHZ010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12958_length_6943_cov_8.492160"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="P5549_09185"
     CDS             complement(<1..521)
                     /locus_tag="P5549_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW13285.1"
                     /translation="MEKIWMKHWPPDMAKEVVLTEGKIPIFEYLRINARRTPAKPAII
                     NYGREISYQELDETSDQFANYLLDLGLVKGDRVGLFLGNSPQYIIAHFGIQKMGGIVC
                     PCSPLFKEMELSYEVNDAGMKCLVTVDLFMPIVAKALPNMPSLLQVVTTNFHDYLPAE
                     PTLPLVDYMQVPK"
     gene            847..1497
                     /locus_tag="P5549_09190"
     CDS             847..1497
                     /locus_tag="P5549_09190"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRW13286.1"
                     /translation="MKGGAIVIDRRTEILAVAARLFREKGYISASLDDITDEIGISKP
                     AVYYYFEAKEDILFEICMTHINRLHNQAKAIAASDFDIAEKIAKLFANHMMQFHLQRD
                     GAETYLREAAHLSLERRKFASDTLKLYEAFICSHVDQAIHQGVFRTVDSKMVVRGIGG
                     MLNWLGNWYTPDGNCDIEYIIATHIDFIMHGLLAEPGRDAGRFGYIIEPGSCLKKV"
     gene            1544..1681
                     /locus_tag="P5549_09195"
     CDS             1544..1681
                     /locus_tag="P5549_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HRW13287.1"
                     /translation="MSLPLYTFVAGITGVFLTMILLQVFITLSSKLAIYIESKTTIQE
                     K"
     gene            1706..2818
                     /locus_tag="P5549_09200"
     CDS             1706..2818
                     /locus_tag="P5549_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRW13288.1"
                     /translation="MDVILNIWSSMAFDSLYWGNFVMWGIAFLFIYLAIKKDFEPLLL
                     LPIAFGILLVNLPSEIMTPGEGLLWKFYHYGEEWAVIPPLIFLGLGAMTDFGPVIANP
                     KTLLLGAGAQGGVYLTFFGALAAGFTVYEAATIGIIGGADGPTTIFLAAKLAPHMMGV
                     CAVAAYSYMALVPIIQPPIIRVLTTEKERKIRMKSLRKVSKLEKIFFPIITCIIVCLL
                     VPDAAALIGMFCFGNLLKESGVVDRLSNTAQNELMNIAVIFLGISVGATMPAEVFLSP
                     KVIYIFVLGVVAFAGATAIGILLAKVMNLFLKEKINPIIGAAGVSAVPMAARVAHKIG
                     QEADKKNYLLMHAMGPNVAGVIGTAVAAGMFISVLK"
     gene            2904..3116
                     /locus_tag="P5549_09205"
     CDS             2904..3116
                     /locus_tag="P5549_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRW13289.1"
                     /translation="MAAIESPMSGKVLEILVEVGQSIAEDDEVIILEAMKLENPIYAP
                     EGGVVKEIKVKVGQQISEGEVIAELE"
     gene            3203..3598
                     /locus_tag="P5549_09210"
     CDS             3203..3598
                     /locus_tag="P5549_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13290.1"
                     /translation="MAKRNSVSSKKVVCRKCGQTVNEDAHHCLKCGTGAPGINSRCPN
                     CHGENYVYHKYGYALIRALLATCVLGPLGPVFGFIGYNRTECVCLACQQGWFPFMPEE
                     QRGKFNTYFGEEGRKSRKFKKIPDNCFKR"
     gene            3678..4949
                     /locus_tag="P5549_09215"
     CDS             3678..4949
                     /locus_tag="P5549_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HRW13291.1"
                     /translation="MEWQIWFAGILFLIVYAIIISEKIHRTIIALFGAALLIVLGVIS
                     QHEAISYIDFNTIGLLFGMMVIVGITRKSGVFEFVAIKAAHSTGGDPVRIMIALSIVT
                     AIASALLDNVTTVLLIVPVTISICAALEVNPVPIFIAEIISSNIGGTATLIGDPPNIM
                     IGSATGLGFMDFVINLSPIIVVIFIVTIIILKFIYKKNLQTTEENKARLMGMNPQEAI
                     IDPQLMKKALLVITVVVIGFVIHQYLGLESATIAITGAALLMLITREDPEEALVTVEW
                     ATLFFFVGLFILVGGLEKVGIIENMAREAVALTEGNVTLSVVFILWFSAIASAFIDNI
                     PFVATFIPLIQEMGQIGHMNVMPLWWALSLGACLGGNGTIIGASANVIVTGIAAREGH
                     PITFLGYMKIAFPLMILSIAIATVYLLLFFV"
     gene            4971..5957
                     /locus_tag="P5549_09220"
     CDS             4971..5957
                     /locus_tag="P5549_09220"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRW13292.1"
                     /translation="MTLRESVLLALKEENGNWFSGEALSVTLRVSRTMIWKQIKQLRT
                     EGYIVEASSRKGYRLVACPDILSAEEVLPGLKTEVFGRKDYFYYQEIDSTNQQARILA
                     SQGYPEGTVVVADAQTAGRGRRGRDWYSPAGQGIYMSLILRPHMPLKEISRISLMPAV
                     ALAETLQEELDLPAQIKWPNDILINKKKIAGILSEAITDMDGIEFVVIGMGLNINNAE
                     SDFPDDFRNHPTSVLAEKKQNASRIKILQCLLAKLEQHYQRLQAGDFTEILMAGRRLS
                     MVIGQQVSLETNQGLVTGRAVEIDENGFLLVKDGSGTLHTVISGEIEVKSDK"
     gene            6067..6603
                     /locus_tag="P5549_09225"
     CDS             6067..6603
                     /locus_tag="P5549_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRW13293.1"
                     /translation="MLELNSPLRRMVYAAMFGALTAIGAFIFIPLQPVPISLQSLFCC
                     LAGMLLGSYTGALSQIVYLLLGVMGLPVFAGGKAGLGTLFGPTGGYLLGFVVAALVIG
                     KMVEGKREAGVLWIGAALLLGHLVIYILGSLQLSWVADLSLTKSILIGVAPFIPGDLL
                     KIAAAVLLGRKLQPLLRR"
     gene            6608..>6943
                     /locus_tag="P5549_09230"
     CDS             6608..>6943
                     /locus_tag="P5549_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW13294.1"
                     /translation="MQALIQVNNLIYQYTEGAAVLQDINLTINEGEYLALIGPNGCGK
                     TTLIRHFNALLQPSCGDVMVGGFNTRHAKNKMEIRRLAGMIFQNPENQIVGMTVEEDV
                     AFGPANLGYP"
BASE COUNT         1856 a         1315 c         1768 g         2004 t
ORIGIN      
        1 tgtttgggaa cctgcatata atcgaccagc ggaagggtag gttcagcagg caaataatcg
       61 tggaagttgg tggtaacaac ttgcagcaag gaaggcatat tgggcaacgc cttggcgaca
      121 atcggcataa acaaatcaac cgttactaaa catttcattc cagcgtcgtt gacttcgtag
      181 gagagttcca tttctttgaa aagcggggaa caaggacaga caataccacc cattttctga
      241 ataccgaaat gggcaatgat gtattgtggg ctgttgccca agaacaatcc aacccggtcg
      301 ccttttacca atcccaggtc gagcaggtag ttggcaaact gatcagaagt ttcatccaat
      361 tcctgataag agatttcccg accataattg atgatggctg gtttggcggg agtgcgcctg
      421 gcattgattc tgagatattc aaagatcggt atttttcctt cggttaatac gacttctttt
      481 gccatgtccg ggggccaatg tttcatccag attttctcca tgaacatttc actccttttt
      541 taattaaaca ttgatacggt cagccgtagg aattgttttt tcaccttccc ccttgttttt
      601 taattcatgc cggaagttta agcttgtttt tttatttttg cccaacgcca acatttgaat
      661 tctgattaag tgcctttgga atcgataatt ctcaactatg gatatgatga ttataagcaa
      721 atgatgcttg acaaaccaaa gcgttttact tactgttaag taagtactta taatacttac
      781 ttaacagtaa gtatagcaat taaatattct tcgtacaaga aaagaatctg ttttattctt
      841 ttataaatga agggaggagc gattgttatc gatcgaagaa cggaaatttt agcggtagca
      901 gccaggttat tcagagaaaa agggtatata tcagccagtc tcgatgatat aactgatgaa
      961 atcggcatca gcaaacccgc ggtctactat tatttcgaag ccaaggaaga tattctgttt
     1021 gaaatctgta tgacccatat caatcgcctt cataatcaag ccaaggcgat tgcagcgtca
     1081 gattttgata ttgctgaaaa gatagccaaa ctttttgcca atcacatgat gcagtttcat
     1141 ttgcagagag atggtgccga aacatatttg cgtgaagccg cccatctctc actggaaagg
     1201 cgtaagtttg ccagtgatac gctaaaactg tatgaggctt tcatctgcag ccatgttgat
     1261 caagccatcc accaaggtgt gtttcgtacg gttgacagca aaatggtcgt tagaggaatc
     1321 ggcggaatgc tgaattggct gggaaattgg tacacaccgg atggaaattg tgatattgag
     1381 tatattatcg caacccatat tgattttatt atgcacggtt tattggctga gcccgggaga
     1441 gatgccggca gatttggtta tataattgag cctggaagct gtctgaaaaa ggtataatta
     1501 cagcgttggc ttgcacaagg aagtcaagaa aggaggacaa tccatgtcac tgccgctata
     1561 tacttttgtc gctggaatca cgggcgtgtt tcttaccatg attctgcttc aggtatttat
     1621 aacactgagt tccaaattgg caatctacat tgaaagtaaa acgactattc aagaaaaata
     1681 attggcttaa aggggtaata cggcaatgga tgtgatttta aacatatggt ccagtatggc
     1741 ctttgatagt ctctattggg gaaacttcgt aatgtgggga atcgcctttc tgtttattta
     1801 tctggccata aagaaagatt ttgaaccact cttactattg cccatcgctt tcggtatact
     1861 gctggtgaat ctgccttctg agattatgac tcccggggaa gggttactgt ggaagtttta
     1921 tcattacgga gaagaatggg cggtaatccc ccccttgatt tttctcgggt tgggtgcgat
     1981 gactgatttt ggcccggtga ttgccaatcc aaagacatta ttgctgggtg ccggagccca
     2041 gggaggcgtt tatcttactt tctttggggc actagcagca gggttcacgg tttacgaagc
     2101 agcgacaata ggaataatcg gcggtgctga cgggcctacc acgatctttt tagcagccaa
     2161 attggcgcca catatgatgg gtgtttgtgc ggtagcagcc tattcctata tggctctggt
     2221 gccgattatt cagcctccga tcattagagt gcttacgacc gaaaaagaac gcaagatccg
     2281 gatgaaatca ctgcgcaaag tatccaagct ggagaaaata ttctttccga ttattacctg
     2341 cattatcgtt tgtttgctgg tccccgatgc agctgcgcta ataggcatgt tctgctttgg
     2401 caatttgctg aaggaatcag gagtggttga caggttgagc aacacagcgc aaaatgagct
     2461 gatgaatatt gcggtaatct tcctgggcat atcagttggg gccacgatgc cggcggaagt
     2521 attcctgagt cccaaagtaa tttatatatt tgtcttgggc gtggttgcgt ttgctggtgc
     2581 aacagctatc ggcattttac tggccaaggt tatgaatctc tttcttaaag aaaaaattaa
     2641 tcccatcatt ggtgccgcag gtgtttcggc ggttcccatg gcagcccggg tggctcacaa
     2701 aattgggcag gaagctgaca agaaaaacta tctgctcatg cacgccatgg ggccaaatgt
     2761 agctggcgtg attggaacgg ctgtagctgc cggtatgttt attagtgtcc tgaagtaaac
     2821 tggtttcgga tatatattgt tttttggttg caatgtatca ggtaaaatat aaaatggtat
     2881 tttaaaataa ggaggaatcg aaaatggcag caattgaaag tcctatgtcg ggaaaggtgc
     2941 tggagatttt ggtggaagtt ggacagagca tcgccgaaga tgatgaagtc atcattctgg
     3001 aagcaatgaa actggaaaac cccatttacg caccggaagg cggagttgtg aaagagatta
     3061 aggtcaaggt cgggcagcag attagtgaag gcgaagtcat tgccgaattg gaataacaaa
     3121 aggagatact cttaagattg gaagttggat atcgattcag cttccaatct tatgtgagtt
     3181 ggtttaggtg ttgattcagg ttatggcaaa aagaaatagt gtcagcagca aaaaagttgt
     3241 ttgcaggaag tgcgggcaga ctgtgaacga ggatgcccac cactgcttga aatgcggtac
     3301 aggagcacca ggtatcaaca gccgctgccc caattgtcat ggggagaatt atgtctacca
     3361 taaatacggt tacgcgctta ttagagcttt gttggcaacc tgtgttttgg gaccactggg
     3421 gccggtgttt ggttttatcg gctacaatcg gaccgaatgt gtctgtcttg cctgccaaca
     3481 aggctggttt ccgtttatgc cggaagaaca acggggaaaa ttcaatactt attttggcga
     3541 agaaggacgc aagagcagaa agtttaagaa gatccccgac aattgcttta aaagataggt
     3601 ttgatgagca taagatattt tctttgcaat ccaaaaatgt atgagaaaaa atgttaacac
     3661 aaaaggagtg aaaagatatg gaatggcaga tatggtttgc ggggattctt ttcttgattg
     3721 tgtatgcaat catcatatcc gaaaaaattc atcggaccat tattgccctt tttggtgccg
     3781 ctctgctgat tgtgctggga gtgatcagtc agcatgaagc aattagttat attgatttca
     3841 atacgatcgg tctcttgttt ggcatgatgg ttattgtagg gattacccga aaaagcgggg
     3901 tttttgaatt tgtagccatc aaagcggctc attcaaccgg gggtgatccc gttagaatta
     3961 tgattgcgct gagcattgtt acggcaattg cttcggctct gttggataat gtaacgacgg
     4021 ttttattgat cgtgccggta acaatatcga tttgtgctgc cttggaagtc aacccggtgc
     4081 caatttttat tgccgaaatt atatcttcaa atattggcgg aacggcaacg ctgatcggtg
     4141 accctcccaa tatcatgatc ggcagtgcta ccggactggg atttatggat ttcgttatca
     4201 accttagccc gatcatcgtg gtaattttta ttgtgacgat tattattttg aagtttatat
     4261 ataaaaagaa tctgcaaaca accgaagaaa acaaagctcg cctaatggga atgaatcccc
     4321 aagaagccat tatagatccc caactaatga aaaaggcatt gctggttatt accgttgttg
     4381 ttatcggctt tgttattcac cagtatctgg gactggaatc agcaaccatc gccattacag
     4441 gggctgcttt gcttatgtta attacccggg aagatcccga agaagcgctg gtaacggttg
     4501 aatgggcaac gcttttcttt tttgtaggtc tgtttattct ggtaggggga ctggagaaag
     4561 tcggtattat tgaaaatatg gcccgggaag cggtggctct gacggaaggg aatgtaacac
     4621 tatcggtggt ttttattctc tggttttctg cgattgcctc agcttttatc gataatattc
     4681 cttttgtcgc cacctttatt cctttaattc aggaaatggg gcagatcgga cacatgaatg
     4741 ttatgccgtt gtggtgggct ttgtcgctgg gagcctgttt gggcggcaat ggtacgatta
     4801 ttggggcttc agccaacgtt attgttactg gcatagcggc ccgggaaggt catcccataa
     4861 cgtttttagg ttatatgaaa attgcctttc ccctaatgat tttgtcaatt gcaatagcaa
     4921 ccgtgtattt attactcttc tttgtataat atttttgggt ggtgaaaaga atgaccctgc
     4981 gcgaatcagt attgctggcc ttaaaagaag aaaacggaaa ttggttttcc ggtgaagctt
     5041 tgagtgtaac cctgcgtgta tcacgcacca tgatctggaa acagatcaag caattaagga
     5101 cggaaggtta tatcgttgaa gcttcttcca gaaaaggcta tcggttggtt gcctgtcccg
     5161 atatcttgtc cgccgaggaa gtgcttccgg gcttaaaaac tgaagtgttc ggcagaaaag
     5221 attatttcta ttatcaggag attgattcca ccaaccagca agcgcggatt ctggcttccc
     5281 agggatatcc cgagggtacg gtggtggtgg ctgatgcaca gacagcaggc cggggccgaa
     5341 gaggtcgcga ttggtattcg cccgccgggc aggggattta catgtctttg atcctccggc
     5401 cgcacatgcc gctcaaggaa atttcccgga tttccctcat gcctgcagtt gcattggcgg
     5461 aaaccttgca ggaggagctg gatctgcctg cccaaatcaa atggcccaat gatattctaa
     5521 tcaacaagaa gaaaattgcc gggatcctgt ctgaagccat taccgatatg gatgggattg
     5581 agtttgtcgt gattggtatg ggcctgaaca taaataatgc agaaagtgat tttccggatg
     5641 attttcgtaa ccatcctacc tcggttctgg cggaaaaaaa gcagaacgca tcaaggataa
     5701 aaatcttgca atgtttgctg gcaaaattgg agcaacatta tcaacggttg caagcgggtg
     5761 attttacaga aatactgatg gcaggcagaa ggttgtccat ggtcattggg cagcaggtca
     5821 gcctggaaac caaccaggga ttggtaactg gtcgggcagt agagatagat gagaatgggt
     5881 tcttgctggt gaaggatggt tcaggaactt tacatacagt gatttccggg gagattgagg
     5941 ttaagtcgga caagtagcaa gaattgattg taaacaaaaa tactaaataa tgttgacaat
     6001 aaaaatgatc catatgataa tgatatggat cattttttat caggtgtttg cttgaggagg
     6061 aagattatgc tcgaattaaa cagtcctttg cgaagaatgg tgtatgccgc catgtttggg
     6121 gctttgacgg caatcggtgc ctttattttt attccattgc agccggttcc catcagtctg
     6181 caatcacttt tttgctgtct ggcggggatg ctgttaggaa gttataccgg agcgttaagc
     6241 cagattgttt atcttcttct tggcgtcatg ggcctgccgg tttttgccgg gggaaaagcc
     6301 gggctgggga cgctgtttgg tccgacgggt ggatatttat tgggatttgt tgttgctgcc
     6361 ctggtaatcg gcaaaatggt tgagggcaaa cgggaagcgg gcgtcctttg gataggcgct
     6421 gctctgttgc tgggccatct ggtgatatat atactgggca gtttgcagtt gtcctgggtg
     6481 gcagatctat cgctcaccaa gagcattttg attggggtcg caccgtttat accaggagac
     6541 ttgctgaaaa tagctgcggc tgttttgctg gggcggaaac ttcagccatt gttaaggaga
     6601 tagaaggttg caagcattaa tccaagtaaa caatctcatt taccaatata cagagggagc
     6661 ggccgttttg caggatatca atcttacgat caatgaggga gaatatcttg cgctgatcgg
     6721 tcccaacggt tgtggaaaaa caacgctgat cagacatttc aatgctttat tgcagccttc
     6781 atgcggagat gtgatggtcg gtggattcaa tacccgccat gccaagaaca aaatggagat
     6841 tcgacgtttg gccgggatga tctttcagaa tcctgaaaac caaatcgttg gtatgacggt
     6901 ggaggaggat gttgcgtttg gacctgccaa ccttggctat cct
//
LOCUS       DAOTHZ010000095         6868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_13135_length_6868_cov_9.111992, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000095 DAOTHZ010000000
VERSION     DAOTHZ010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13135_length_6868_cov_9.111992"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /gene="tilS"
                     /locus_tag="P5549_09235"
     CDS             complement(<1..933)
                     /gene="tilS"
                     /locus_tag="P5549_09235"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRW13295.1"
                     /translation="MIHAVKAYIASQELIGRGNQVIAAVSGGPDSMALLHILMELRPV
                     LGFHLVAAHVNHGLRPEAALEQQFVEEQCQSWGITCYAKSIDVSGLARQAKTSLEDAG
                     RQARYGFFYELLQILQADVVATAHHQDDQAETVLLHLLRGAGMPGLRGILPRNGRLVR
                     PLLSHSKHDLLAYLQDNQIAYCIDESNQDESFLRNRIRHQLIPLLQAEYNPQITENLS
                     RLAEIIRAENDFLQQIVQGYWQKLLCAQDNVSVEMDAEGIKTLPLAVQRRLTMMALSS
                     VGGSQGWESRDVEKVMALLHKPGSAKILQLKKGII"
     gene            complement(1010..2899)
                     /locus_tag="P5549_09240"
     CDS             complement(1010..2899)
                     /locus_tag="P5549_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRW13296.1"
                     /translation="MEDKRLQIPGSQFNISEHPNVDIFFNAYRHTSIIMSILNLDDYT
                     YADVNDSWERTFGYSRQEVVGRQARSLGLWIDEVQDEQKIREIKNHQGIQNFEVQYRS
                     REGRIGYGQVSSQLIAIDGYHFCLNAMLDITDRKQIESKLHQSEENLKNILFTLVSIS
                     DPFYAVDAEWRVIYANQKVQEWWQKTQEDLYGQYLWEILVEPEKPRICKLINRAMQKR
                     VAVTYDTFSVKMASWFEVSVYPARDGGLSFYCKDITARKQAEQALQESEARHRRLAEK
                     LREVDLAQSEDRFYKIFNLTPDMIVIMDMEDDTFIEVNQATLDVYGFTREEMLGHTGA
                     EIGAIKAVDPSYLLFGEELKANGIIKNFEVTTTNKTGKRIDLLISSVPINLSGKQCRL
                     SVLKDISEKKRYEQEMARLDRLNLVGQMAASIGHEIRNPMTSVRGFLQMFGQGNSGIN
                     KEYYDLMIEELDRANEIITEFLSLAKDKATDLRAHKLGSIIDRIRPMLEAEALLHGKK
                     IKIITGSETQLLLDENEIRQLILNMVRNGMDAMAAGGTVTIVIEENVGIVVLTIADQG
                     CGIAPEIVDLIGTPFVSSKENGVGLGLAVCYRIAERHGAHIAFDTGKAGTVFKVYFPP
                     HAQTV"
     gene            complement(3112..5583)
                     /gene="spoIIE"
                     /locus_tag="P5549_09245"
     CDS             complement(3112..5583)
                     /gene="spoIIE"
                     /locus_tag="P5549_09245"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HRW13297.1"
                     /translation="MLERLEIYPYQRMEEEAGSRKMRRKIGIVWGQPLQLRKMIPAGK
                     VKFTAERLGQLLSWENLLLAGGALILSRAFILGELLPFIFAFVAVFAVQNLQRSLIGL
                     LFSLLGLATVLPASATVINMVALGVMVWILNTEKIQSGKSWWGIPVITAAVVLVIKSA
                     ALMISGLNFYQEMVIVFEALISGIISYVLLHCLEVIQQKKPMASFSFEEMGAFMVLGM
                     GLIMGLGDLQLFGLEVSSILCRLAIMIAAYLWGTGGGTIMGVMAGILPSIASSLFAQT
                     LGMYSISGLLAGIFRSFGRLGVIVGYMLGTLALSMFMADSQLIVLGLWETGIASIVFM
                     LLPESLKEKLPTQSLGRLSGLTTANSQTDWHLNETIGNRIHHLAQVFDELSSTLTGTE
                     EDPVRLPGNSCLNYLYDELSNGFCEGCHRFTHCWNHDYQRTAQEILELFCQAESAGQL
                     NYEDMPLSLKKRCLHGRELVTTVNYLFDNLRINEYWSEKMGESRQLVARQLKGVGQLV
                     RNLADEIEVDSSIDVELREQLLKACKRLGINLKDVSPVRCKGELLSINVISASCVDGS
                     GCDLSIAPALSSALGQKWEVCQKNCPAIMGRGACDFTLKRAFNYQVVSAVAQVAREEK
                     SGDSLIVKTLDEGKELVVLSDGMGVGAKACEESNTAVKLLLDLLAGGFDKELALKTIN
                     SVLLLRSRGESFTTMDMMLIDLFNAELDFIKIASAPAYIKRGKQITVLSSSSLPIGIL
                     EEVEVNSERKTLLPRDIILMVSDGVMEASREQNGDIWIPRLLAQIRETDPHIIAQMVI
                     NRALSLAAGKPGDDMTAICLRLDIA"
     gene            complement(5991..6860)
                     /locus_tag="P5549_09250"
     CDS             complement(5991..6860)
                     /locus_tag="P5549_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRW13298.1"
                     /translation="MAATACAAGKSKAELPLLKMIVLGILAGAYIGFGANLATKIGSM
                     DTLLPGGTPGGQFLFGAVFSVGLMLVVIAGAELFTGNNMACAISAMNGQASWGGLLIN
                     WVVVFVANFIGSVLLVYIVFWGGYWGQADAAGAITLTAMGSKALAIAKGKLALTWGQA
                     FCRGILCNWLVCLAVWMAFAAKDIVGKIFAIFFPIMAFVASGFEHSVANMFFIPMGIT
                     IANANPDMVAAATKMVDGVPVSVFNMTADQVVQFFSYANFFSANLLPVTLGNIVGGAI
                     FVGGFYWLAYLRK"
BASE COUNT         1814 a         1851 c         1424 g         1779 t
ORIGIN      
        1 gatgattccc ttttttaact gtagtatttt tgcagaaccc ggcttgtgca gcaaagccat
       61 aaccttctca acatcccgtg attcccagcc ttgcgaaccg ccaacggatg acagcgccat
      121 catggtcaat ctgcgctgca cggccagtgg cagtgttttg ataccctcgg catccatttc
      181 cacactcaca ttgtcttggg cacagagtaa cttttgccaa taaccctgca cgatctgttg
      241 caaaaaatca ttttctgcac gaatgatttc cgccagacgg cttaagtttt ctgtgatttg
      301 gggattgtac tccgcctgca acagcggaat taattgatgt ctgatgcgat tgcgcaaaaa
      361 gctttcatct tggttgctct catcgatgca ataggcaatt tggttgtcct gcagataggc
      421 cagcaaatca tgtttgctgt gcgataaaag cggccggacc aaccggccgt tgcggggcaa
      481 aatccctcgc aaacctggca tgccggctcc cctgagcaga tgcaaaagga ccgtttctgc
      541 ctgatcatcc tggtgatgag cagtggcaac aacatccgct tgcaatatct gcagcaattc
      601 ataaaaaaaa ccatagcgag cctgacgacc ggcatcttcc agggaagttt tggcttgacg
      661 ggccaatccg ctgacatcaa tggattttgc ataacaagta atcccccagc tttggcattg
      721 ttcttcgaca aactgttgtt ctagcgctgc ctccggtcgc aacccgtgat tgacatgggc
      781 tgccactaaa tgaaaaccca acaccgggcg caactccatt aaaatatgca agagggccat
      841 tgaatctggc cctcctgata cagctgcaat tacttggttg ccgcgcccga ttaattcttg
      901 gctggcaata tatgctttga cggcatgaat catagctgct cctcttctcg ctcagttact
      961 actatcctac tcaagatacg gtaattgtca agccaagggg acaaagcatt cagactgtct
     1021 gggcatgtgg gggaaaatac accttaaaaa cagttcctgc tttccctgta tcaaatgcaa
     1081 tgtgcgctcc gtgtcgctct gcaatccggt aacagaccgc caatcccaat cccactccat
     1141 tctccttgct gctaacgaag ggcgtcccaa tcaagtcaac gatctcagga gcaatcccgc
     1201 agccctggtc ggcaattgtc agcaccacta tgccaacgtt ttcctcaata acgatggtta
     1261 cggttcctcc ggctgccatc gcatccatcc cattgcggac catattcagg attaactgac
     1321 gaatttcatt ttcatccagc aacagttggg tctcacttcc cgtaatgatc tttatttttt
     1381 tcccgtgtaa caacgcttca gcctccaaca tcggtctgat tctgtcaatg atggaaccaa
     1441 gcttgtgcgc tcgcagatcg gttgccttat ctttggccag acttaaaaac tcggtaataa
     1501 tttcattggc ccggtccagt tcttcgatca tcagatcata atattctttg tttataccac
     1561 tgtttccctg gccgaacatc tgtaaaaaac ctcgcactga agtcattgga ttgcgtatct
     1621 catggccaat cgaagcagcc atctgaccca ccagattgag ccgatccaga cgagccattt
     1681 cctgttcata acgtttcttt tccgagatgt ctttcaagac ggagaggcga cactgtttgc
     1741 cgctcagatt aatgggtacg gaagaaatta gtaaatcgat ccttttaccg gttttgttgg
     1801 tggtcgtcac ctcaaaattc ttaataatgc cgtttgcttt cagttcttcc ccaaaaagaa
     1861 ggtaggatgg atcaaccgct ttgattgcgc cgatttcagc gccggtatga ccaagcatct
     1921 cctcccgggt aaaaccatag acatccaaag tcgcctggtt gacttcaata aaggtatcgt
     1981 cttccatatc cataataacg atcatgtctg gtgtgagatt gaaaattttg taaaaccggt
     2041 cctctgattg ggcgagatcg acctcacgca gcttctcagc cagccgacgg tgacgcgctt
     2101 cgctttcctg cagtgcttgt tcagcttgct tacgggcggt tatgtccttg caatagaagg
     2161 acaaccctcc gtcgcgggct ggataaacac tcacctcaaa ccaactggcc atcttcacac
     2221 tgaacgtatc atacgtcacg gcgacccttt tctgcatagc cctgttgatc agtttgcaga
     2281 tacgtggctt ttcaggctca acgaggatct cccacagata ttgcccatac aaatcctcct
     2341 gcgttttttg ccaccattcc tgcaccttct gattggcata aataacacgc cattcggcgt
     2401 caacggcata aaacgggtca ctgatggaca ccagggtaaa caaaatattt ttcagatttt
     2461 cctcactttg atgcagcttg ctttcaatct gcttgcgatc ggttatatca agcatggcat
     2521 tcagacaaaa atgatacccg tcaatcgcaa tcaattggga tgatacctgg ccataaccga
     2581 ttcttccctc tctgctgcgg tattgaactt caaagttttg tattccctgg tgattcttta
     2641 tttccctaat cttttgctca tcctgaacct catcaatcca taaacccagc gatctggcct
     2701 gtcgaccaac cacttcctga cgggaataac cgaaagtacg ctcccagctg tcattgacat
     2761 ccgcataggt ataatcgtcc agatttaaaa tcgacatgat tatactggta tggcgataag
     2821 cattaaaaaa tatatccaca ttgggatgct cgctgatgtt aaattgactg ccgggtatct
     2881 gcagacgttt atcctccatc acgtcgttct cctttaactg actgaatcaa tgattctttt
     2941 gctgatgatt cgctcggcca atgttatgtt atcacagcct cattccaatc attggatact
     3001 tttgctaaat atccttctga aataccgaaa gttttctgga tttttagcaa aataaatctc
     3061 cctcaccgat gactgacgat gagagagatt tgatttttta tgcgattacc atcaggctat
     3121 atccaatctt aagcagattg cggtcatatc atcgcccggc ttgcctgccg ccagactcag
     3181 cgctcggttt atcaccatct gggcaatgat gtgcggatct gtttcccgaa tctgggccaa
     3241 taaacgcggt atccagatat caccattctg ttcccgggaa gcttccatca cgccatcact
     3301 gaccatcagg ataatgtccc gaggcagcag cgttttcctc tcactgttga cttccacctc
     3361 ttccaggatt ccaatcggca gggaggaaga actaagtacg gtaatttgtt tgcctctttt
     3421 aatataagcc ggtgcgctgg caattttaat gaaatcaagc tcggcgttga ataaatcgat
     3481 cagcatcata tccatggtgg taaagctctc gccgcgggag cgcagcaaca aaacagaatt
     3541 aatcgtcttg agggccaatt ctttgtcaaa ccctccggcc agtagatcca acagcagctt
     3601 gacggcggtg ttgctttctt cacacgcttt ggcgccaact cccataccgt cacttaagac
     3661 gaccagttcc ttgccttcat ccagcgtctt gacaatcagg ctgtcaccgc tcttttcttc
     3721 ccgggcaacc tgggccacag cactcacaac ctggtaatta aaagcccgtt taagggtaaa
     3781 atcacacgct ccgcggccca ttatggcggg acagtttttc tggcagactt cccatttttg
     3841 tcccaaggca gacgacaggg ccggtgcaat gcttaaatca caaccgcttc cgtctacaca
     3901 ggatgcgctg atcacgttga tcgacaataa ttctcccttg caacggaccg gactgacatc
     3961 cttcaaattg attcccagcc gtttacaggc tttcagcaat tgttcccgca attccacatc
     4021 aatggaagaa tccacttcaa tttcatccgc caggttgcgg accagctgac ccacaccctt
     4081 gagttggcgt gcaaccagtt ggcgtgattc gcccattttt tccgaccagt attcattgat
     4141 gcgcaggttg tcaaaaagat agtttaccgt ggtcaccaat tcacgtccgt gcagacagcg
     4201 cttttttaat gacaatggca tatcctcata attcaattgc cctgcacttt ccgcctgaca
     4261 aaagagttcc aggatctctt gtgcggtacg ctgataatca tgattccagc aatgcgtgaa
     4321 gcggtggcat ccttcacaga aaccatttga taattcatca tataaatagt tcagacaaga
     4381 attgcccggc aaacgcaccg ggtcttcttc cgttccggtt agcgttgaac tcaattcatc
     4441 aaagacctgg gcaagatgat gaatgcgatt gccgatagtt tcattaagat gccaatctgt
     4501 ctgggagttt gcggtggtga gaccggataa cctgcccaga ctctgtgtcg gtagtttttc
     4561 ttttaatgac tccggcaaaa gcataaaaac aatactggca atgccggttt cccataaacc
     4621 cagcacaatc agctggctgt ctgccataaa cattgacaac gccaaggttc ccaacatgta
     4681 tccgacaatg acccccagtc ggccaaagct gcgaaatatt cccgccagca gtccggatat
     4741 ggaatacatg cccaaggtct gggcaaaaag gctggacgca atggaaggaa ggatccccgc
     4801 cattaccccc atgatggttc ctcctccggt tccccagaga taggcagcaa tcataatcgc
     4861 cagacgacac aggatactgc tgacctccaa gccgaacaat tgaagatcac cgaggcccat
     4921 gataagaccc attcccagaa ccataaaagc gcccatttct tcaaaactaa aacttgccat
     4981 gggtttcttt tgttggatta cttccagaca atgcagcaat acataactga tgattccgct
     5041 gatcaaggct tcaaaaacga tcaccatttc ctgataaaaa tttagcccgc tgatcatcaa
     5101 agcagcactt tttatgacca aaacaacggc tgcagtgatt accggtatcc cccaccagct
     5161 cttgcctgat tgtatttttt ccgtgttcag aatccacacc ataaccccta aagcaaccat
     5221 gttaatgacg gttgcactgg cgggcaaaac cgtagccaac cccaacaaac tgaacagcag
     5281 cccaatcaag ctgcgctgca gattttggac agcaaaaaca gcgacaaaag caaatataaa
     5341 gggcaacagt tcgcccagaa taaaagcccg ggagagaatc aacgcccccc cggccagaag
     5401 caggttttcc cagctcaaca attggccaag ccgttctgca gtaaatttca ctttaccggc
     5461 gggaatcatc tttcttaact gtagtggttg gccccagact ataccgatct tgcgtctcat
     5521 ctttctggaa ccagcctctt cctccatcct ctgataagga tatatttcca gacgttccaa
     5581 catatcgtcg cctccttgtg actgaacctg agtttaaaca ttatagcaag gaacccggtt
     5641 ataaaaatgt cagcccaagg gataagactt tattttattc aaacaaattc tctgtataaa
     5701 attcctgcag tcgcagctta ctccagactt caacatttat ccaaggatta tacaatcata
     5761 tccggcccac acctcaagac gttttacata gccgtacgat tttccttcca tttccaacgc
     5821 acgatagcct tgataacagc cggagattca aaatctcccg gtctcgacgt cagacatcgc
     5881 agttggctga atctgtgcac ataaataagg gagtggccat ctgaacaggc cactcccaaa
     5941 atctttattt tgaatactcc caaaacattt accaaaatga taataaaaaa ctattttctc
     6001 agatatgcca gccaatagaa tccgccgaca aagattgctc cgccgacaat gttacccagg
     6061 gtaaccggca gcagattggc gctgaagaaa ttcgcatagc tgaagaactg caccacttga
     6121 tcagccgtca tattgaatac gctgaccggt acaccgtcta ccattttggt agctgcagcg
     6181 accatatcgg ggttggcatt ggcgatggtg atacccatcg gaataaagaa catgttggca
     6241 acgctatgtt cgaagccgct ggcaacgaag gccatgatcg ggaagaagat agcaaaaatt
     6301 ttgccgacaa tgtctttggc ggcaaaagcc atccaaaccg ccagacaaac cagccagtta
     6361 cagagaatac cgcggcaaaa cgcctgtccc caagttaatg ccagtttccc tttggcgatt
     6421 gccagtgctt ttgatcccat ggcagtaagt gttattgccc ccgcggcatc ggcttgtccc
     6481 cagtagccgc cccagaagac gatataaacc aatagaactg aaccaatgaa gttggcgacg
     6541 aatacaacga cccagttgat aagcaagccg ccccaagagg cttgaccgtt catggccgaa
     6601 atagcacagg ccatgttgtt gccggtaaac agttctgctc cggcaatgac taccagcatc
     6661 agaccgacgg agaatactgc gccaaacagg aattggccgc ccggggttcc tcctggtaaa
     6721 agtgtgtcca ttgatccgat cttggttgcc aggttcgctc caaatccaat gtaggcacct
     6781 gccaaaatac ccagaacaat catcttgaga agtggcagtt cagccttgga tttgcctgct
     6841 gcacatgcgg ttgccgcaat ctctgcag
//
LOCUS       DAOTHZ010000096         6843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_13188_length_6843_cov_9.456394, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000096 DAOTHZ010000000
VERSION     DAOTHZ010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13188_length_6843_cov_9.456394"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(156..277)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(400..1266)
                     /locus_tag="P5549_09255"
     CDS             complement(400..1266)
                     /locus_tag="P5549_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRW13299.1"
                     /translation="MKHSIDCLPCLLRQAVRTVKTHLHDEDVQRQTLQKVMAEMLGVG
                     DDVSAPFIAHKIQNILVEVLGNPDPYKEDKGYYNCEMIKLEPELLRIRNASDDPFATA
                     LKLAAAGNIIDFGPVDGLSREKVLEIIDTTLETSLSQEALHSLHNDLHNAVTLLYLGD
                     NAGEIVFDKIFVDTIKNSFPKLRIYFATRGKPVLNDITEDDAYLVGMDQLAEIINNGS
                     NIPGTDLAYTTPSFKKIFYQADVVIAKGQGNFETLYGCRKANLYYLFLCKCNLFMSRL
                     GAKQNDLVLLKE"
     gene            complement(1359..1910)
                     /locus_tag="P5549_09260"
     CDS             complement(1359..1910)
                     /locus_tag="P5549_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRW13300.1"
                     /translation="MPFYEFEEKTPVLDPSCFVHPDAILIGDVTIGKHCFIGAGAVLR
                     GDYGSIVISDGANVQENCTLHAQPGSVAFIGANVDVGHGCIIHGPCAIGSNVTIGMGS
                     TICDNCQIGDNCFIGAGSVLPSKLVIPDGKLAFGNPAKVIKDTNEYQRTFTKMAIDVY
                     KDLCERYRKSFKLIEDATRLYRG"
     gene            complement(2111..3304)
                     /locus_tag="P5549_09265"
     CDS             complement(2111..3304)
                     /locus_tag="P5549_09265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW13301.1"
                     /translation="MVESSELQNKKDRYYFTDTINLEEGQVYISKLDLNIENDAIEVP
                     YKPMIRFGAPVYDQHHQLMGIVMLNYLAENLIDEFKNIGTDSSGRIYLLNSAGYWLSS
                     TDPEEEWGFMFAEKKDRLFSRKYPSEWERINRNESSFITPNGLFTASAVILADKFLSS
                     DRVILDESDWKVVTIIGGTDPEGYILSANWMDRVKRVITDNLFDFGLVLLISFSISLF
                     IEIRKQSYLRIKYLSEYDAFTGVLNRGTGLKRLHRSITENKRQRDRISICMIDINGLK
                     MVNDILGHDAGDELILTIVHEIAAGIRETDYIFRHGGDEFIVVCHDTDQESAECIWQR
                     IAARFNQINQEGNRPYNVSASHGIINAGDYQYKTVEELIKMADQIMYEEKRKIKQQIT
                     VVRNT"
     gene            3980..5299
                     /locus_tag="P5549_09270"
     CDS             3980..5299
                     /locus_tag="P5549_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13302.1"
                     /translation="MCDTMVALGNATADGRVLFAKNSDRQPNEPHVIIQIPRRHYAPG
                     TRLQCTYIEVDQAEETLAVLLLKPSWIWGCEMGCNEFDLNIGNEAVFTREKYGEPALT
                     GMDMIRLALERCRDSKAALDFLIELLERYGQGGNCGYRKPFTYHNSFLIADPHRAWVL
                     ETAGPYWAAEQVTGVRNISNGLSIGSAMTRCHQELIEHARQKGWCKSEDDFDFARCYK
                     DPLYTHFSGSAQRGSLCRTELEKQHGKIDAATMMSVLRSHHPATEGRQFSQSSLKSVC
                     MHGGGIIGDHTTGSYVASLSESDCVYWVTGASTPCLAIFKPLWLMDHNPLLYSEAEQE
                     QAVAYWQKRELFHRMVINQQIRNLDEYIARRDRLEAEWQQAVNVLQTERADDGKKRDF
                     MQNAWQQEDELIAALLRENSAQPVRIKGSPYFRRYWRQQEKTRQRGL"
     gene            5301..6431
                     /locus_tag="P5549_09275"
     CDS             5301..6431
                     /locus_tag="P5549_09275"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018306848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRW13303.1"
                     /translation="MRILIAPDSFKESLTAAQAAACIAEGIRKVLPDAEITQLPLSDG
                     GEGLTETLVTPMNGRMLAQEVTAAMGNRVTACYGIAGAQTAILEMAAASGLALVPPAQ
                     RNPLQATTYGTGELIRAALDQGCRRLIIGIGGSATNDGGAGMARALGVRMMNTQGNDI
                     AEGPAGLLELEAIDMAAIDARLAHTEIKVACDVTNPLCGPQGASRVYGPQKGADAAAV
                     ELMDRALARMAEVIKRDLGLAIADLPGAGAAGGLGAGLAAFAGATLQPGLDLVLEILQ
                     FDALLAAGQDLIITGEGEINGQSLYGKVPVGVARRAREQGIPVLALVGNIGPNAEQVY
                     AEGMTSIMSIAPGPITREQSMARAGELLTNAAERAIRLIERK"
     gene            complement(6566..6843)
                     /locus_tag="P5549_09280"
                     /pseudo
     CDS             complement(6566..6843)
                     /locus_tag="P5549_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641824.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1801 a         1674 c         1569 g         1799 t
ORIGIN      
        1 aaaccaataa aaccgctaga attaaaccaa taactttttt catttcacta cctcctcatt
       61 ttttctgctc aatagaattt actgccctcc tcccccaaaa aataagcgac ccggggaaag
      121 atcccaggtc gcattttata cgaacattaa aattgcagga agcaatataa tctcctttcc
      181 accctgggag gcacagccgt ttccagctat accctatcgg ccaaataccc gttgatatat
      241 atacattagg gcaagaggct cggagcaata ttaagctata taacaacaca aatcaaaaat
      301 gtggacatat gcccacaaac ataataagcc tatttaattg atatttcaag ataatgttta
      361 aaagcaacat ttatatccgt atttgccatt gtgttgaaat cattctttga gcaagaccag
      421 atcgttttgt tttgctccca atctactcat gaaaaggtta catttacaga ggaataaata
      481 ataaagatta gctttacggc aaccgtataa ggtttcaaaa ttgccctggc cttttgcaat
      541 tacaacatca gcctggtaaa aaatcttttt aaaggaaggg gtagtataag cgaggtcagt
      601 tcctggaata ttactgccat tgttgataat ttcagccagc tgatccatcc caaccagata
      661 agcatcatct tccgtaatgt cgttcaaaac cggtttgccg cgtgtggcaa aataaattct
      721 cagcttggga aagctgtttt ttatagtatc gacaaaaatt ttgtcaaaga caatctcccc
      781 ggcattgtca cccagataaa gcagggtaac agcattgtgc aaatcattgt gcagggaatg
      841 aagagcttcc tgggaaagac tggtttccaa agttgtatct atgatttcca gaactttttc
      901 ccgggaaaga ccatcaacag gaccgaaatc aataatgttg cctgctgcag ccagtttcaa
      961 ggccgtcgca aaaggatcat cactagcgtt gcgaatacgc aacagttccg gctccagttt
     1021 gatcatttcg cagttataat accctttgtc ttctttatat ggatccgggt tgcccaacac
     1081 ttctacaaga atattctgaa tcttgtgggc gataaacgga gcgctgacat cgtctcccac
     1141 acccagcatt tctgccatca ccttttgcaa cgtttggcgt tgcacatctt catcatgaag
     1201 atgtgtttta actgtgcgca ctgcttgtct aagcaggcaa ggcaaacaat caatactgtg
     1261 cttcataaat accctccatt ccagcagatg ggtgtgttat aaaatgctgt agatctgctg
     1321 tatatatggc aaatcaacta ccggtttaaa accccgtctt aaccgcgata taaacgggta
     1381 gcatcttcaa tcagtttgaa ggacttgcgg taacgctcac acagatcttt atagacgtca
     1441 atcgccattt tggtaaaagt acgctggtat tcattggtgt ccttgatgac cttggcagga
     1501 tttccaaaag cgagtttccc atcgggaata accagtttgg aaggcaaaac actgcctgct
     1561 ccaataaaac aattatcccc aatctgacag ttgtcacaga tcgttgaccc catcccaatg
     1621 gtcacattac tgccaattgc gcagggacca tgaatgatgc atccgtgacc tacgtctacg
     1681 ttggcgccga taaaagccac ggaacccggt tgggcgtgaa gggtacaatt ttcctgtacg
     1741 ttggccccat cactgattac aatgcttccg taatcacctc gtaaaactgc accagcccca
     1801 ataaaacaat gtttaccaat ggttacgtca ccaatcagaa tggcatcagg atgaacaaag
     1861 caggaaggat caagaaccgg ggttttttct tcaaattcgt agaatggcac agacaaatct
     1921 ccttaataag ttattattgc aacattaaat aacataagcg agcatatgct caacaccaga
     1981 ttcaatatag tatttttttc tttaggtagt caagagcaaa attgtttgac aatccattaa
     2041 acaccgttgg gcctgccgtt gataatccat ccgggggctc gtgaatatat gctgcttttt
     2101 gccgaaggca tcatgtatta cgaacaactg taatttgttg ctttattttt ctcttttctt
     2161 catacataat ctggtctgcc attttaatca gttcctcaac cgttttgtat tgataatcgc
     2221 ctgcgttgat aattccatga cttgcgctta cattatatgg tcggtttccc tcttggttta
     2281 tttggttgaa tctcgccgca attctctgcc aaatacactc agcactttcc tgatcggtat
     2341 catgacaaac gacaataaat tcatcacctc cgtggcgaaa tatgtaatcc gtctctctga
     2401 ttcctgctgc aatctcatga acaatcgtca aaattaattc atcgccggca tcatgtccca
     2461 gaatgtcatt gaccatttta agaccattga tgtcgatcat gcaaatactg attctgtctc
     2521 tttggcgttt attttccgtg atggaacgat gtagccgttt tagaccagtt cctctgttta
     2581 aaacgcctgt aaaagcatca tattcagaga ggtatttgat tctcaaataa gactgttttc
     2641 gtatctcaat aaacaaagaa atcgagaatg atataagcaa aaccaatcca aaatcaaaca
     2701 aattgtccgt aatcaccctc ttgactcgat ccatccagtt tgcggacagt atataacctt
     2761 cgggatctgt accaccgatt atcgtaacga ccttccaatc actttcatcc agaatcaccc
     2821 gatcactcga taagaattta tctgccagta tgactgccga agccgtaaag agaccattgg
     2881 gagtgatgaa agagctttcg ttccggttta ttctttccca ttccgaagga tactttcgag
     2941 agaagagccg atcttttttt tcagcaaaca taaatcccca ctcttcttcc gggtcagtgc
     3001 tggaaagcca gtagcctgcg gaattcagca gataaatgcg gccgctgctg tctgtaccga
     3061 tatttttaaa ctcatcaatg agattttcag ccagatagtt taacattacg atccccatga
     3121 gttggtgatg ctgatcataa accggtgctc caaaccggat catgggttta taaggaactt
     3181 caatcgcatc gttctcgata tttaagtcaa gtttggaaat ataaacctgt ccttcctcta
     3241 aatttatggt gtcagtaaaa taatagcggt ccttcttgtt ctgaagttca gaagactcaa
     3301 ccacataggc gcccttttca cgggaataat tgatccgaat tttctcatct ccgttgctat
     3361 cgatgaatcg aatttggtca tatatttttt gctcttgcgc aaactcccgc caccgttcaa
     3421 taattctcga cttttcatca gcggcttgac ccccggtttc atatgtgttg gtcaaataat
     3481 agagatccgc aataattcca ttgatttctt tgcccaggag ccgtgattca gtggctgtga
     3541 tccagttttc tttggctgtt agttcatcaa ttcgtatacg atccatattc ttttgcagga
     3601 aatattgaaa acccaccaaa gaaattgaca tcactaaaaa tataatcaga gaatatttca
     3661 ctgctcccgt ataattgaat tgattctgcg gagattgttg caagaaaaaa cctcccttct
     3721 gataatcgag tttaaatctt tcctatgttt ttaggtgctt ttgggctttg tttaattaca
     3781 taattccaat cagctattca taatcctgca atcattctaa accaaattgt ctcaagtgcg
     3841 ttatcataac tatataagtc cgagtcacaa tagcctgtct gcctgatcaa agcccaatga
     3901 cccagtgtat ttaaaccaat acagaaataa taagatagaa taaaaacatg gcccaaggaa
     3961 tgtgggggag gtaatatgca tgtgtgatac gatggtcgct ttgggaaacg ccacggcgga
     4021 cggccgggtt ctgttcgcca aaaacagtga ccgccagccc aacgaaccgc atgtaatcat
     4081 ccaaatacca cggcgccatt atgcgccagg cacccggctg cagtgtacct atatcgaggt
     4141 ggatcaggca gaggagacct tggcggtgct cttgttgaaa ccctcctgga tctggggatg
     4201 cgagatgggt tgtaatgaat tcgacctgaa cattggcaat gaagcggttt ttacccggga
     4261 aaaatatggg gagccggcct tgaccggtat ggacatgatc cgcctggccc tggaaaggtg
     4321 ccgcgacagc aaagcggcgc tggactttct gatcgagctg ctggaacgct acggtcaggg
     4381 cggcaattgc ggctaccgca aaccttttac ctatcataat tccttcctga tcgccgaccc
     4441 gcaccgcgcc tgggttttgg aaacggccgg cccatattgg gctgccgaac aggtcacggg
     4501 ggtgcgcaat atctccaacg gtctcagtat cggcagcgcc atgacgcgtt gtcaccagga
     4561 actgatcgaa catgcccgcc aaaagggatg gtgcaaatct gaagacgatt tcgactttgc
     4621 ccgctgctac aaagatcccc tctatactca tttcagcggc tccgctcagc gaggcagtct
     4681 ttgccggacg gagctggaaa agcagcacgg caaaattgac gcagccacca tgatgtctgt
     4741 tcttcgttct caccacccag ccacggaagg ccgccagttc agccaatcct ccctgaaaag
     4801 cgtatgcatg catggcggag gcatcatcgg tgatcatacc accggcagtt atgtcgcatc
     4861 gctgagtgag tcggactgcg tttactgggt taccggtgcg tccacaccct gcctggcgat
     4921 tttcaaaccg ctctggttga tggatcataa tccgcttctg tattcggagg cggagcagga
     4981 gcaggccgtt gcttactggc aaaaacggga attgtttcac cgcatggtca tcaaccagca
     5041 gattcgaaat ctggatgaat atatagcaag acgggatcgg ctggaggcgg aatggcagca
     5101 ggcggttaac gttttgcaga ctgagcgggc tgatgacggc aagaaacgcg actttatgca
     5161 aaatgcctgg cagcaggaag atgaactgat tgcggctttg ctgcgtgaaa acagcgcaca
     5221 gccggttcgc atcaagggtt ccccctattt ccgccgttac tggcggcagc aggaaaagac
     5281 ccggcaaaga ggtctttgat atgcgcatac taattgcccc cgactctttt aaggaaagtt
     5341 tgaccgccgc ccaggctgct gcctgcattg ccgaggggat acgaaaagtc ctgccggacg
     5401 cggagatcac ccagctgcct ttgtcggacg gcggagaagg cttgaccgaa acgctggtca
     5461 cccctatgaa cggccggatg ctggcgcagg aggtcactgc tgccatgggc aaccgggtga
     5521 cggcctgcta tggaatagcc ggcgcgcaaa ccgccatcct tgaaatggcc gcggcctccg
     5581 gcctggccct ggtgccgccg gcgcagcgaa acccgctgca ggccaccact tatggtaccg
     5641 gcgaactgat ccgggcggcc ctcgatcagg gatgccgccg tctgatcatc gggattggag
     5701 gcagtgccac caatgacggc ggagcgggca tggcccgggc cctgggggtc agaatgatga
     5761 acactcaggg gaatgatatc gctgaaggtc ccgccggttt gctggaactg gaggccattg
     5821 acatggctgc gatcgatgcg cgtctcgctc atacggaaat aaaagtggcc tgtgatgtga
     5881 ccaatcccct ctgcggtccg cagggagcct cccgggttta cggtccgcaa aaaggggctg
     5941 atgcagctgc agtagagctc atggatcgag cgctggcaag aatggcggag gtaataaagc
     6001 gcgatctcgg cctggctatc gctgatcttc cgggcgcagg tgcagcgggc ggattgggtg
     6061 caggactggc ggcttttgcc ggagccactt tgcagcccgg gctggatctg gtgctggaga
     6121 ttctgcagtt tgatgctctg ctggcggccg ggcaggatct gatcatcacc ggagaaggag
     6181 aaatcaacgg ccagagtctt tacggcaaag tcccggtagg cgtggcccgc cgggcccggg
     6241 aacaagggat accggtgctg gccttggtgg gcaatatcgg cccgaatgca gaacaggttt
     6301 atgccgaggg tatgacctcc atcatgagca ttgctcccgg tcccatcacc cgcgagcagt
     6361 ccatggcccg cgccggagaa ctcctgacca acgccgccga acgggccata cggttgatag
     6421 agcgcaagta gaacgcaagt ggggacggtt cgagaccact gaaaaagtac cctttttaaa
     6481 tcgcctcggc gtctgcctta atccaaattt gctggttaat cagcaaccaa cggtataaaa
     6541 acattaaaaa tgcgctaaat aaagctttta aagcggatac catcctggct atccgcttta
     6601 aatttaccgc cagggccgtt agtttggcct gtatgcggac actttttaga ccgtagcctt
     6661 tggctcggtc caatccgtgg tatcttttca actctgcatt cttgggttcg attttcgctc
     6721 gcttgcggta ttcttcaagg aattccggag ttttggctct ttgactgtac tcatataact
     6781 ccggggtatt tacactgatt ctaagtagtt tagctattcg cttacttttc cctacgcatt
     6841 cgg
//
LOCUS       DAOTHZ010000097         6573 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_13877_length_6573_cov_7.271249, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000097 DAOTHZ010000000
VERSION     DAOTHZ010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13877_length_6573_cov_7.271249"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="P5549_09285"
     CDS             <1..1493
                     /locus_tag="P5549_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HRW13304.1"
                     /translation="AELARRVEKLAAYLIKTGVQKGDRLAYVFAPCPEFMYLYLAAAR
                     VGAVITGMGTRQTEPEMTYVLNNSQASHIFSLYSMYDIDYQDRFASILPQCTTVKSLI
                     IAQGQARLPQAVSLEEILQGDYTAYLDELQRREAELDADDGLLIVYTSGSTGTPKGAL
                     MSHRNIIHMSLVELAMCDGNGDDIWLNHLPVNHVSGATEVGATALVANATQVMEPFNP
                     ARTLEIIQTEKVTILGQVPTMFAIEFALPNYADYDFTSLRAVVISGAPAPVETLKKMK
                     ETMCDNCYNCLGLTEVSGLITFTERGASLETLNLTVGKCAPDFAMKLTDPDRREVAPG
                     EVGEIAYRGTSVIKEYFMLPEATKAAFDDEGWFYSGDLGLIDADGNLRMVGRSKEIYI
                     TNGYNVYPAEVEDAIMKFPGVMLCACVGVPHPVAGEIGRAYVVPLPGHTIDTEQLKEF
                     LKDHLSSYKIPREYVLREMLPMTMLGKIEKKLLRQEVAAEAAASRE"
     gene            1527..2267
                     /gene="fabG"
                     /locus_tag="P5549_09290"
     CDS             1527..2267
                     /gene="fabG"
                     /locus_tag="P5549_09290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRW13305.1"
                     /translation="MRLNAKTAIITGAGKGIGASIAKRFAAEGARLAICDINETLIRQ
                     TADELQTSGTEVFSMTVDVTDRNMIKDMVQKVLERFATIDILVNNAGILKDVMLHKMN
                     EEDWDRVMNVNMKGTFNMTQAVAAIMREKGYGKIINISSASRFGNVGQTNYAASKEAL
                     VGFTRALAKEMGPKGINVNAIAPGTIMSDMYWGIPEHVREVMKIITPLGRAGTPEEVA
                     NLCLFLASDEASYITGQVIHCDGGIFMP"
     gene            2592..3350
                     /locus_tag="P5549_09295"
     CDS             2592..3350
                     /locus_tag="P5549_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HRW13306.1"
                     /translation="MTKTEDKGYRQIEDLDTAALARLILNMSQRMAVHSTLWFRETEH
                     QLGMKRALDMLENVNAQYGKLEMDRLGKVLGFEVTDGIPRPLLDMPAEKLTELAGEMG
                     KNWLAMDGIWFQAIENTYGMNDAKRSNDSCWHRFSQVEGRMIKNFLGLPERAGLDGLK
                     RALAFRMYARLNKQSIIEESPHSIVFQMNDCRVQSARKRKGLADYPCKSAGLVEYSRF
                     AWEIDERIQTECIGCPPDDHPDDWFCAWRFTLPE"
     gene            complement(3506..4885)
                     /locus_tag="P5549_09300"
     CDS             complement(3506..4885)
                     /locus_tag="P5549_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13307.1"
                     /translation="MARDFQQMYKERLRTAEQVAQVVKSGDWVDYAMFNGKPVAFDKA
                     LAARKDELQDVKVMVAVTIPPIPEVVMKDFQGETFTYMDLQFSAASRIMQENCGGVFY
                     HPTNFGESEDYFDWVHSDPGKIGSTPRKIFAIRTTPMDKNGFFNFGIHNSVSYAQIKS
                     AQYTVVEVNENIPLALGGAREQVHISEVDFIIESENEPMFELPAIEATEVDRKIAEHV
                     LEHITDGSCIQLGIGSMPNLLGKLINDTDLKDLGGNTEMLVDAFMDMWESGKMNNRRK
                     NVDDGKLVYTFTLGSQRLYDWIDHNTGLASYNVGYVNHPSRVAAIDNLISINQALQVD
                     LYTQVNAESSGFKQISGNGGMSDFTLGAFWSKGGRSLICLPSTYKTKDGEMRSRIVPT
                     LGMGSITTVPRQMVHIIVTEYGWESMKAASTWARTEKIIGLAHPDFRDELIKEAEKMK
                     IWRRSNKIL"
     gene            5125..5361
                     /gene="tlp"
                     /locus_tag="P5549_09305"
     CDS             5125..5361
                     /gene="tlp"
                     /locus_tag="P5549_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein Tlp"
                     /protein_id="HRW13308.1"
                     /translation="MKNKPDDRRDNVEKLQANITHTIQNIELAEETMAETSNQQMKQD
                     ISAKNDRRRDALANMRAEIRDEALDQRQEDESLT"
     gene            complement(5468..>6573)
                     /locus_tag="P5549_09310"
     CDS             complement(5468..>6573)
                     /locus_tag="P5549_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HRW13309.1"
                     /translation="AIRMWVTQEDMDSFNTAITTAQTAHDDAAATDASLMTAGNNLNT
                     ALIVFRNAMQPGSKAVYITAAPGQTTVANPFTQTIMLTLSAGSFVDGIGVGDITLAGD
                     FAGLSASNVTKSSADTLSIQLSGTLVRNTGTGIITIAAAATTENNIMAAQVAVNPLPP
                     PATALNGLSIREGNDGSGQELIMGFDPYNWNYALQVEKETSSVTVAVTAVPGTTAKVY
                     LGAVEVSGGEVALAEGENIVKVIVSEDNRSDRVYEITIQRGPLDECFIATAAFGSKFE
                     PAVAMLRHFRDQYLLTNKLGTAFVKFYYQHSPPIAAWIAGSDGLRMVARMALAPMIAV
                     VYLLYHPTAAAMTGLILVLLMLFWMKRRRRNFI"
BASE COUNT         1676 a         1670 c         1606 g         1621 t
ORIGIN      
        1 atgctgaact ggcacgcaga gtggaaaaac tggcggctta cttgattaaa acaggagtgc
       61 agaagggtga ccggttggcc tatgtgtttg ctccctgccc tgaattcatg tatctgtacc
      121 tggcagcagc ccgggtaggt gctgtcatta ccggcatggg aacccgccaa accgaaccgg
      181 aaatgaccta cgttctgaac aattcccagg ccagtcatat ctttagtctt tattccatgt
      241 atgacatcga ttaccaggat cgtttcgcca gcatcctccc gcaatgcacg accgttaaaa
      301 gcttgatcat cgcccaaggt caggcccgtc tgccccaggc ggtcagtctg gaagagatcc
      361 tgcaaggtga ttatactgcc tatctggacg aactgcagcg gcgggaagct gaacttgatg
      421 ctgacgacgg cttgctgatc gtttatacct cgggcagcac cggcaccccc aaaggcgcat
      481 taatgtccca ccgcaatata atccatatga gtctggtgga actggccatg tgcgacggga
      541 acggcgatga catctggctc aatcacctcc cggtcaacca tgtcagcgga gccacggaag
      601 tcggtgcgac cgctctcgta gccaatgcca cccaggtaat ggagccgttt aatcccgctc
      661 ggaccctgga gattattcaa accgaaaaag tcaccattct cgggcaggta ccgaccatgt
      721 ttgccattga attcgctctg cccaattatg ctgattatga ttttacctca ttacgtgcgg
      781 tggtgatcag cggtgcgccg gctccggttg aaaccctgaa aaaaatgaag gaaaccatgt
      841 gcgacaactg ctacaactgt ctcggtctga ctgaggtatc cggtctgatt acttttacgg
      901 aacgcggcgc cagcctggaa acattgaacc tgaccgttgg caaatgcgcc cccgattttg
      961 caatgaagct gactgacccg gaccgccgcg aagtggctcc gggagaagta ggagaaatcg
     1021 cctatcgtgg cacctcagtc atcaaagaat actttatgct gccggaagcg accaaagcag
     1081 cctttgatga tgagggctgg ttctattccg gtgatctggg gctgatcgat gcggacggca
     1141 atctgcgcat ggtgggacgc tccaaggaga tttacatcac caatggttac aacgtttacc
     1201 ccgccgaagt ggaagatgcc atcatgaaat ttcccggcgt catgctctgt gcctgtgtcg
     1261 gcgttcccca cccggtcgca ggagaaatcg gacgggccta tgttgtcccc ctgcctggcc
     1321 ataccatcga tactgagcag ttgaaggaat tcctcaagga tcatttaagc agctacaaga
     1381 tcccgcgtga atatgtgctg cgggagatgc tgcccatgac catgctgggc aaaattgaaa
     1441 agaaactgct gcgccaggaa gtagcagcag aagccgcagc atcgcgggaa tagatctgac
     1501 tgaatgcgta taagggaggg atttagatgc gtttaaacgc caaaacagct atcatcaccg
     1561 gcgccggcaa aggcattggt gccagtatcg ccaaacgctt cgcagccgaa ggtgcccgcc
     1621 tcgccatttg tgatatcaat gaaacgctga tcagacagac ggctgacgaa ttgcaaacga
     1681 gcggtacaga ggttttcagt atgacggtcg atgtaaccga ccgtaacatg atcaaagaca
     1741 tggttcaaaa ggtcttggaa cgatttgcaa ctatcgatat cctggtcaac aacgctggca
     1801 ttttgaaaga tgttatgctg cacaaaatga atgaagaaga ctgggatcgg gttatgaacg
     1861 taaatatgaa gggaactttt aacatgaccc aggcggtcgc ggctattatg cgggaaaaag
     1921 gctatggcaa aatcatcaat atctcttctg ccagtcgctt cggcaacgtc gggcagacca
     1981 attatgccgc cagcaaagaa gcacttgtcg gttttacgcg ggcactggcc aaggaaatgg
     2041 ggcccaaggg cataaatgtc aatgccatcg caccaggcac catcatgtcc gatatgtatt
     2101 ggggcattcc cgaacatgtc cgggaagtga tgaagatcat cacccctttg ggaagggcgg
     2161 gaaccccgga agaagttgcc aacctttgcc tctttttagc cagcgatgag gcttcctata
     2221 ttactggtca ggtcattcac tgtgacggcg gtattttcat gccctgatgt ttcatgaccc
     2281 atgccaaggg ttacctttat cggcataatt tctgtaatgg attctgcgca ttcgcgataa
     2341 atttactttt tttgagccag gaaccaccct ggctcattct tttttctgtc tctgcgcaaa
     2401 tatttttatg ttagaattta atctgatagt aatcacgcat gagactgctg tgtttgccag
     2461 gaggttcttg attgatcaag ccggcggtcc aaacagtcgt tacccggtca ccaacctgtt
     2521 cagaaggatc tgaacagttg atgtattcct atgcagtgaa gaccacggtc aacaacagga
     2581 ggtaatgaaa aatgacaaaa acagaagaca aaggttaccg gcagatagaa gatctcgata
     2641 ctgcggcatt agcccggctg atcctcaaca tgtcgcagcg catggccgtc cacagcaccc
     2701 tctggttccg ggaaacagag caccagttgg gaatgaaacg agccctggat atgctggaaa
     2761 acgtcaatgc ccaatacgga aaactggaga tggatcgttt gggcaaagta ctgggttttg
     2821 aagtaaccga tgggattccc cgtcccctgc tggacatgcc ggcagaaaag ctgactgaac
     2881 tggccggaga aatgggcaaa aactggctgg cgatggacgg catctggttc caggcgattg
     2941 aaaacaccta tggcatgaac gacgccaagc gcagcaacga ttcctgctgg catcgctttt
     3001 cccaggtaga gggacgaatg atcaagaatt tcctgggact gccggaaaga gcgggactgg
     3061 atggattaaa aagagccctg gccttccgaa tgtatgcccg cttgaataaa caatccatta
     3121 tcgaagaaag cccgcatagt atcgtttttc aaatgaatga ctgccgcgtc cagtcagccc
     3181 ggaaacgcaa ggggctggcg gattatccct gcaaatcagc cggcttggtt gaatacagcc
     3241 gctttgcctg ggaaatcgat gaacgcatcc aaaccgaatg tatcggctgc ccgccggatg
     3301 atcatcccga tgactggttc tgtgcctggc gtttcaccct acctgaataa accaggtcag
     3361 ggcatcctct gtctgagctg cccaacctgc cattatccca attgcacatt cggtaccggc
     3421 gcatagtttt ttattatatt cgctgacaga aaagccctca cccacaaaaa taccgtcccg
     3481 gaaatctccg ggacggttgt agttactaga ggattttgtt gctgcgccgc catatcttca
     3541 tcttttctgc ttcttttatc aactcatcgc gaaaatcagg atgcgccaga ccaatgattt
     3601 tttccgttcg cgcccaggtg gaagccgctt tcatcgattc ccagccatat tccgtcacaa
     3661 tgatatgtac catttgacgc ggaaccgtag taatgctgcc cattcccagg gtaggcacga
     3721 tgcgcgaacg catttctccg tctttggttt tgtaggtgga cggcaaacaa atcagactgc
     3781 gtcctccctt ggaccagaag gcccccaagg tgaagtctga cataccccca ttgccggaaa
     3841 tttgtttgaa cccagaactt tcggcattca cctgcgtata cagatcgacc tgcagcgcct
     3901 ggttgatgga aatgagatta tcgatggccg ccacccggct gggatgattg acatagccaa
     3961 cattatatga ggccaatccg gtattgtgat cgatccaatc gtacagcctc tggctgccca
     4021 aggtgaaggt gtaaaccagt ttgccgtcat cgacgttttt gcggcgattg ttcatctttc
     4081 ctgattccca catgtccata aaagcatcca ccagcatctc ggtgttgccg cccaaatctt
     4141 taagatcggt atcgttgatc agtttgccga gcaagttggg catcgaaccg attcccaatt
     4201 ggatacagct gccatcggta atgtgctcca aaacgtgttc agcgattttg cgatctactt
     4261 ccgttgcttc aatggccggc aattcaaaca tcggctcgtt ttcactttca atgataaagt
     4321 caacctctga tatatgaact tgttcgcgtg ctccgcccag cgccagaggg atgttttcat
     4381 ttacttcaac cactgtatat tgcgcacttt ttatctgggc ataagaaacc gaattgtgaa
     4441 tcccgaagtt aaagaatccg tttttgtcca tgggggtagt gcgaatagca aagattttgc
     4501 gcggggtgga gccaatcttg ccgggatcgg aatgtaccca gtcaaaataa tcttccgatt
     4561 ctccaaaatt ggtaggatgg tagaagactc caccacagtt ttcctgcata atccggctag
     4621 cggcactgaa ctgcagatcc atgtaggtaa acgtttcgcc ttggaaatcc ttcataacca
     4681 cttcgggaat tggaggtatg gtgaccgcta ccatcacttt tacatcctgc agttcatcct
     4741 tgcgggcggc gagtgctttg tcaaatgcga ccggtttgcc gttaaacatc gcataatcga
     4801 cccaatcacc ggatttaacc acttgtgcaa cctgttcagc cgtgcgcagt ctttctttgt
     4861 acatctgctg aaaatctctg gccatttaat cccctccaat ataattttat gtaatatatt
     4921 atctgtattt aatatactta ttataataat aactttattt ctgttccact gcgaaatacc
     4981 ttctatttgc gaatatattc gacacccact gcattaattg tttaaacatt gaattaaaac
     5041 aattatttct atttgttata tttcagcctt tatattcaca aaataaactt gacacgagat
     5101 acgcaatgaa gggagaaaat ttctatgaaa aacaagcctg atgatcgtcg tgacaatgtt
     5161 gaaaaactgc aggccaacat cacccatacc attcagaata ttgaactggc agaggaaacc
     5221 atggcagaaa cctccaatca acaaatgaaa caagacattt ccgccaaaaa cgaccgacgc
     5281 cgagacgcac tcgccaacat gcgtgcggaa atccgggatg aagcactcga ccagcggcag
     5341 gaagacgagt cgctcacttg acacttttaa cagttatatc ctttctctga gcagcgcgaa
     5401 aaaaagaggc tgacccttgt tccgatcggt ttgggttcag cctcttcaca atttgtaaca
     5461 aatgctttta gatgaagttt ctccggcggc gtttcatcca gaagagcatc aacagcacca
     5521 ggatcagtcc ggtcatcgct gccgcagtcg gatgatagag cagatagacg acggcgatca
     5581 ttggcgccag cgccatccgg gcaaccattc ttaagccgtc gctgccggcg atccaggcgg
     5641 caataggcgg cgaatgttgg taatagaatt tcacaaaggc tgtgccaagc ttattggtca
     5701 gcagatactg gtcacggaag tgacgcagca tggcgaccgc cggttcaaat ttggaaccaa
     5761 aagccgcggt ggcgatgaag cattcgtcca gcgggccgcg ttggatggtg atttcataaa
     5821 cccggtcaga acgattgtct tcgctgacga ttacttttac gatattctcc ccttccgcca
     5881 gcgccacctc tccgccgctc acctcaaccg ctcccagata cactttggcc gtcgtaccag
     5941 gtaccgcagt gaccgcgact gtaaccgaac tggtttcttt ttctacctgg agcgcatagt
     6001 tccagttgta tggatcgaac cccatgatta actcctgccc gctcccgtca tttccctcac
     6061 ggatactcag gccatttaag gcagttgccg ggggtggaag cggattcaca gcaacctggg
     6121 ctgccatgat attgttctca gtggttgcgg ctgccgcaat cgtaattatg ccagtaccgg
     6181 tgtttctgac aagtgtgcca cttaattgga tacttaaggt gtcggccgag gacttcgtca
     6241 cgttgcttgc agacagccct gcaaaatccc ctgctaaagt gatatctcca accccaatac
     6301 catcaacaaa actgcctgcg gatagtgtga gcatgatggt ttgggtaaag ggattggcca
     6361 cggttgtttg tccgggtgcg gcagtgatat atactgcttt cgagccgggc tgcatggcat
     6421 ttctaaagac aatcagcgcc gtattaaggt tattgccagc cgtcatcaga cttgcatcgg
     6481 ttgccgctgc atcatcatgg gctgtctggg cagtagtgat cgctgtattg aatgaatcca
     6541 tatcctcttg ggtaacccac attcttatag ctg
//
LOCUS       DAOTHZ010000098         6571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_13883_length_6571_cov_6.658226, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000098 DAOTHZ010000000
VERSION     DAOTHZ010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13883_length_6571_cov_6.658226"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="P5549_09315"
     CDS             <1..343
                     /locus_tag="P5549_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRW13310.1"
                     /translation="ENVLLNRESPGFIVNRILLPYINEAIWLYAEGTDIESIDQAMIL
                     RAGMSQGPLQQADMIGLDNLHAVMLGFYNEFRDPKYRPHPVFSTMVRAGYYGKKTGKG
                     FYTYKKGEGSI"
     gene            340..1170
                     /locus_tag="P5549_09320"
     CDS             340..1170
                     /locus_tag="P5549_09320"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRW13311.1"
                     /translation="MTFKAKKSFGGLYGIIAGILVFGFSFWGIGYSLGPDDKALKVLL
                     YIPAYLFLVVYIYLVLGALRLKYVVNDDNFLLIWGVAQKHIAWDEIDEIVQIEGKSNL
                     FPFLSMTWPGYVVGLFQLKGVGPVRMYGTHAKDGFLYLKTQRGFLGITPQDDKLAKLI
                     AEKTGKEVQILDMDTIPVEVKGHDMHTDRYFMLYTRINNVLLILFCLYVAIFFPRSNA
                     DPLIILLVVLAINLYLFNNANAKRLYQFSQQGGYFTLLIGMAVTGIFLILSIVQISFS
                     "
     gene            1357..2421
                     /gene="ychF"
                     /locus_tag="P5549_09325"
     CDS             1357..2421
                     /gene="ychF"
                     /locus_tag="P5549_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRW13312.1"
                     /translation="MQLGIIGMPSVGKTTIFELLINQRDKANSQGKANSAIARIPDER
                     IDRLSAIFKPKKTSYAQLELVDIPGLVPGQEKGSHVFLDSVRKADALLHVVRLFQDET
                     IPYYYEKIDPMRDIAMISYELLLADLDLIEKRIERIHASKKKNQMLKELDLLERLKTT
                     LEDEKPISSLQLDQEEQLIMSTYQFLTSKPLLICLNLDDDDLISLDYPQKEAIADYSR
                     LNDIPVVEVAGTIEKEIAELAEEEKEEFLKEMGIHESGLMKVSHSMYKRLGLISFFTV
                     GEDEVKAWTIEQGTPARKAAGKIHSDIERGFIRAEVVEYHDFIELGSMNAVKEKGLFR
                     LEGKEYVVKDGDIVHFRFNV"
     gene            2580..3938
                     /locus_tag="P5549_09330"
     CDS             2580..3938
                     /locus_tag="P5549_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheC/YheD family protein"
                     /protein_id="HRW13313.1"
                     /translation="MNESGPPKALVKIPPRLAGIITENPQLQIGQTILGIHKFADNIE
                     ISSAIKGNRILISRRLLCKNYPVSMPQAFHQLHFQLLPNKTIKTGPVIGVLIAGKEKP
                     GAPPIPRRTLIYRELSRTAVKKGLFLYYFYAEEVDWEHQWVKGFLYDERSKRNTPWLK
                     AKFRLPDIVYNRISYRHKEDAYGVQAFMNKAARAGIHVFNSRFLNKWEVHQCLEADSR
                     TKEFILPTACFSKPMLTVFLNNYPEFFIKPVANSIGKGIIKVIISESGQISYFRSGKS
                     SRWQECQSREELYHRLSLPQGERYILQKGIQLASIDHRIFDIRAQAQKDGEGLWQFTG
                     AAVRVAAAGKFLTHVPNGGSKRDYDQTIQEVFGPSDSVKEKLDWQLKTICRRVPLVLE
                     EYLGINLAVLSMDIGIDHDGHMWIIEANSKPASFDEYDIRQRHMDLLTDYFLFKAWRG
                     NK"
     gene            3949..5496
                     /locus_tag="P5549_09335"
     CDS             3949..5496
                     /locus_tag="P5549_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13314.1"
                     /translation="MIIKLIYRKNSTGESLLQYFQDNWTDVDCYRFEIRGKKYYEISA
                     TRNQANLKITFNDIRALGNCLNQQICGEILAVNGIFTDFSDAKVSNKSYDLLLFNMTV
                     ISVNQLTAGKSTVTQKYINEKQVPRVCELAKRTLNLLGLDYAMIRISSNPQRKLTVTK
                     ITPSPIMREKDLTNVIDKLEKWVEQCNHQHLDKPEVKLGADPEFMIANSRSQKMIPAS
                     EFFPRQGPVGCDNIRIPRRQQRPIAELRPQPDISPIILHANIKAMLEQANKMAPYKNI
                     KLLAGSQPFSGYSIGGHIHFSNVSLGSHLLRALDNYLALPVFLLEDQQTAIKRRNKYG
                     HLNEYRVKDYGGFEYRTPGSWLVSPEISLAVLCLAKLVSSNYLMLRHNFFLSVKAHQS
                     FYQGDTEYFRELFQELWQDIKQLDEYYLYHQELDIIVQMIKRKQSWDENADLRKTWKL
                     SRNFSKRYATKTNTITSSNYNSRSQTTTSRRSSSQARTLNRTSANNDASSMSYQSQGR
                     YEQPSHV"
     gene            complement(5719..6501)
                     /locus_tag="P5549_09340"
     CDS             complement(5719..6501)
                     /locus_tag="P5549_09340"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRW13315.1"
                     /translation="MNVNDLFSIKGKVAIITGGSIGLGAQMSMGLAEAGANVVVAARK
                     VERCVELCKQIEAETGVRALPVACDTSKAEDCQNLIDATVKEFGTVDILVNNAGITWG
                     ANAINYPMDKWQQILDVNLTGVFQLSAMAARIMKEKGGGKIINISSTTGFGGTYPEIQ
                     NAVAYNTSKGAVITLTKDLAVKWARYKIHVNGIAPGPFVTHMTEKHFSGLGEDKIKMA
                     NPMGRFGSEWDLKGAVVFLASAASDFITGVTIPVDGGSNALS"
BASE COUNT         1909 a         1454 c         1420 g         1788 t
ORIGIN      
        1 ggaaaacgtt cttcttaatc gagaaagtcc gggatttatc gtcaaccgga tcttgcttcc
       61 ctatatcaat gaagccatct ggttgtatgc agagggaact gatatcgaat cgatcgatca
      121 ggccatgatt ctgcgagcgg gtatgagcca aggccctttg caacaggcgg atatgatcgg
      181 tcttgacaac ctccatgcag tgatgctggg tttctacaat gagttccggg accctaaata
      241 tcgtccgcat cctgtttttt caaccatggt aagagcaggc tactacggca aaaagaccgg
      301 taaaggattt tatacttata aaaaagggga ggggagcata tgacttttaa agccaaaaaa
      361 tcttttggcg gtttatatgg catcatcgcc ggtattttgg tttttggttt ttccttctgg
      421 ggcatcggtt attcgctggg tcctgacgac aaggcactga aagtattgtt gtacataccg
      481 gcatatcttt ttctagtggt atatatctat ctggtcctgg gcgcgctccg cttaaaatac
      541 gtggttaacg atgacaattt cctgttaata tggggcgtgg cccaaaaaca tattgcctgg
      601 gatgaaatcg atgaaatcgt tcagatcgag ggaaaaagca atttatttcc gtttctctcc
      661 atgacctggc ccggctatgt agttggcttg tttcaattga aaggagtcgg accggtcaga
      721 atgtatggca cccacgccaa agatggtttc ctttatttaa aaacgcaacg tggttttttg
      781 gggatcacgc cgcaagacga caaacttgcc aaattgattg ccgaaaaaac cggcaaagag
      841 gttcaaatac tggacatgga taccattccg gttgaagtaa aaggccacga catgcatacc
      901 gaccgttatt ttatgcttta tacccgaatc aataacgtat tgttgatatt attctgtttg
      961 tatgtggcaa tttttttccc aagatccaac gcagatccgt tgattatttt gctggtagtg
     1021 ctggccatca acctttatct attcaacaat gccaacgcca agagattata ccaatttagc
     1081 cagcagggag gctatttcac cttattgatt ggtatggcgg ttaccggtat atttttaatc
     1141 ctgtccattg tacaaatcag ctttagctaa acaatagagt agttatattg ctaatggcgt
     1201 ccgcttgttt tccacagtcg gacgcttatt tgcgttccca cgggctatag ggtttacgat
     1261 attatttatt atgtaaacca aagacgacaa actggctatt tttttgttgc cgggctataa
     1321 tagtatttga tttacatagg gaaaggttgt ttgcaaatgc aattaggaat cattggaatg
     1381 ccatcggttg gcaagacgac catttttgaa ttactcatca atcaacggga caaggccaac
     1441 agtcagggca aagccaattc agccattgca cgcattcctg atgaacgtat cgatcgactg
     1501 agtgcgatat tcaagcccaa aaaaaccagt tatgcacaat tagaactggt tgacataccc
     1561 ggcctggtcc caggacagga aaaaggcagt catgtgtttc ttgattccgt tcgcaaagcc
     1621 gatgctttac tgcatgtagt cagacttttt caggacgaga caattcctta ttattatgaa
     1681 aaaatcgatc ccatgcgtga catcgcaatg atcagctatg aattgctatt ggctgatctg
     1741 gacctgatcg aaaaaagaat tgaacgcatt catgccagca aaaaaaagaa tcaaatgctc
     1801 aaagaacttg acctgttgga acgcttaaaa acaaccttgg aagatgagaa gccaatttca
     1861 tcactgcaac tcgaccagga agagcagctt attatgagca cttatcagtt cttaaccagc
     1921 aaaccgctgc tcatttgttt gaacctggac gatgatgacc tgatcagcct ggattatcct
     1981 caaaaagaag cgatcgctga ttattcaagg ctgaacgata tccctgtcgt ggaagtcgcc
     2041 ggcaccattg aaaaagaaat cgccgaactc gctgaagaag agaaagaaga gtttttgaag
     2101 gaaatgggca ttcacgaaag cgggctgatg aaagtcagcc acagcatgta caaacggctg
     2161 ggcttgattt catttttcac ggtgggggaa gacgaggtta aggcgtggac catcgaacag
     2221 ggaaccccgg cccgcaaggc cgccggcaaa attcattccg acattgaacg cggctttatt
     2281 cgcgccgaag tggttgaata ccatgatttt attgaactgg gaagcatgaa cgcagtgaaa
     2341 gaaaaaggct tgttccgtct cgaaggaaaa gagtatgttg tcaaagatgg tgacattgtt
     2401 cattttcgct ttaatgttta aaaaccaatg gcagatttag ttcacaaatt aacagcgaaa
     2461 acgcatttat tataccggga gttgaaatac tcctgttttt ttatggttat ccttgtgtgc
     2521 actttgtatc agacacatac ataatataaa ctatctattt cacttcatga ggtgtgcaaa
     2581 tgaacgaatc tggcccccca aaagcattgg tcaaaattcc tccccgactt gcagggatta
     2641 tcactgaaaa tccccagctg caaattggcc aaaccatact gggtattcat aaattcgccg
     2701 ataatattga aatcagctca gctataaaag gaaatcgtat tttgatttcc cgacgtttgc
     2761 tatgtaaaaa ttatcctgtt tccatgcccc aggctttcca tcaactccat ttccaattgc
     2821 tccccaataa aaccataaaa acggggccgg taatcggagt attgattgcc ggcaaagaaa
     2881 aaccaggtgc ccctcccatc ccccgcagaa ccttaatata cagggaatta agccgaacag
     2941 cagtaaaaaa aggcctgttt ctctattatt tttatgccga ggaggttgat tgggagcatc
     3001 aatgggtcaa aggttttctt tatgatgaac ggtccaagcg gaacaccccc tggctcaagg
     3061 caaaattcag gctccccgat attgtttata atcgcatctc ttatcgccac aaggaagacg
     3121 cttatggagt gcaggcattt atgaacaaag ccgcccgggc tggaattcat gtcttcaaca
     3181 gccgatttct gaataaatgg gaagttcatc aatgcctgga agcagattcc cggaccaaag
     3241 aattcatatt gccaacagca tgtttcagca aaccaatgtt gacagtgttt ctgaacaatt
     3301 atccggagtt ctttatcaag cctgtagcca acagcatcgg caaaggcatt atcaaagtga
     3361 ttatcagcga atctggtcaa atctcatact ttcgttccgg caaatcttcc cgctggcaag
     3421 aatgtcaatc ccgagaagaa ttgtatcatc gtctttccct tccgcaagga gaacggtata
     3481 tcctgcaaaa aggcatccaa ctcgccagta tcgaccatag aatctttgac attcgggccc
     3541 aggctcagaa agatggcgag ggcctgtggc aattcaccgg agcagcagtc agggtagcag
     3601 cagcagggaa atttctaacc catgttccca acggtggcag taaaagagat tatgatcaga
     3661 cgattcagga agtatttggt ccatctgatt ccgtcaagga aaaattggat tggcagttga
     3721 aaaccatctg ccgcagagtc cccttggtcc tggaagaata tttgggtatt aatctggccg
     3781 tattatcgat ggacatcggt atcgatcatg acggccatat gtggattatt gaagccaact
     3841 ccaaaccggc cagtttcgat gaatatgaca ttcgtcaacg ccatatggat ctcttgacag
     3901 attacttctt gtttaaggct tggcgaggaa ataaatgaga gggatggcat gattataaaa
     3961 ctgatatacc gaaagaattc caccggcgaa agtctcctgc aatactttca ggataactgg
     4021 acggatgttg attgttatcg atttgaaatc agaggtaaaa agtattatga aataagcgca
     4081 acaagaaacc aggccaatct caagattact ttcaatgata tcagggcatt ggggaattgt
     4141 ctgaatcaac aaatctgtgg cgagattttg gcggtaaacg gcatattcac agatttttcc
     4201 gacgccaagg taagcaacaa aagctatgat ctgcttttgt tcaacatgac agtaatttcg
     4261 gtcaaccaat tgactgccgg caaaagcacc gtaacccaga agtatataaa cgaaaaacaa
     4321 gtcccccggg tttgtgaatt ggccaaaagg acactcaatt tgttggggct ggattacgcc
     4381 atgataagaa tcagcagcaa tccccagcga aaattgaccg ttacaaaaat cactccttcc
     4441 ccgattatgc gcgaaaagga cctgactaat gtaatagata aattggaaaa atgggttgaa
     4501 cagtgtaacc atcaacatct tgacaaacct gaagtaaaac tgggcgcaga cccggaattc
     4561 atgattgcca acagccgcag ccaaaaaatg atcccggctt ctgagttctt tccccgtcaa
     4621 ggaccggtag gctgtgacaa tattcgtatt cccagacgtc agcaaagacc cattgccgaa
     4681 ctcaggccgc aacctgatat ttcacctatt atacttcacg ccaacatcaa ggccatgttg
     4741 gaacaggcca ataaaatggc gccctataaa aacattaagc ttttggcagg aagtcagcca
     4801 ttttccggct attctatcgg cggacatatt catttcagca atgtcagtct gggcagtcat
     4861 ctgctgcggg ctttggacaa ttatctggca ctgccggtat tcctcctcga agaccagcaa
     4921 acagcgatca aaaggcgtaa taaatatggc catttaaatg aatatcgagt taaggactac
     4981 ggcggatttg aatatcgaac ccccggcagt tggctggtat caccggaaat cagcctggca
     5041 gttctgtgtc tggccaaact ggtcagcagc aattatctaa tgctgcgaca taatttcttt
     5101 ttatctgtta aagcccatca atcattttat caaggcgata ctgaatattt tcgtgaactg
     5161 tttcaggaac tttggcagga tatcaagcag ttggatgaat attatctcta tcaccaggaa
     5221 cttgacatca tcgtacaaat gataaaacgc aagcaatcct gggatgaaaa cgccgatttg
     5281 cgaaaaactt ggaaattaag cagaaatttc agcaaaagat atgcaaccaa aaccaacaca
     5341 ataacgtcat ctaattataa cagccgcagt caaaccacaa ccagccgacg ctcaagcagc
     5401 caagcccgga cattgaatcg tacttccgcc aacaatgatg catcatccat gtcttatcaa
     5461 tcacagggac ggtatgagca accatcgcat gtttaatgaa aactgcaggg ttaaggcctt
     5521 tttaataaaa aaccattatt ccccgcttgg ttttaataat tcccataatg aacattatgt
     5581 aaactagtcc gcggatgcca ttctgtgact ggttctcttt tttttatatt aaaagcggat
     5641 actgtgtccc ctatcgggtt gccagtatcc gcttttttat tttctgtcgt tcatttaatg
     5701 attacaaaat gcagtctttt agcttaaagc gttggagcct ccgtcaacgg gaatggttac
     5761 cccggtgatg aaatcagagg cggcggaggc caggaatacg actgctcctt tgagatccca
     5821 ttcactgccg aatcggccca ttggattggc catttttatt ttatcttcac ccagtccgga
     5881 gaaatgcttc tcggtcatgt gggttacaaa gggaccgggg gcaatcccgt ttacgtggat
     5941 tttatagcgg gcccatttta ctgccaggtc cttggtaaga gtgatcactg cgcctttgct
     6001 ggtattatag gcaacagcat tctggatttc tggataggtt ccgccaaatc cggtagtgga
     6061 ggaaatgtta atgatcttgc cgccgccctt ttctttcatg atccttgcag ccatggcaga
     6121 aagctggaat actccggtca aattgacgtc caaaatctgc tgccatttgt ccatcggata
     6181 attgattgca ttggcacccc aagtgatgcc tgcgttgttg acaaggatat ccactgtgcc
     6241 aaattctttt accgtagcgt caattaagtt ctgacagtct tctgctttgc tggtatcaca
     6301 cgcaacagga agagcccgaa cgcctgtttc tgcctctatt tgcttgcaca gttcaacaca
     6361 tctttccacc ttgcgtgctg ctactacaac attggcacca gcttcagcca gtcccatcga
     6421 catttgggca cccaatccaa tggatccgcc ggttataatt gcgacttttc ctttgatact
     6481 gaataaatcg ttaacgttca tattctttgc caacctccaa taaaattatt tcattgttca
     6541 aatgttacca ttgcttttgg aatctttcaa t
//
LOCUS       DAOTHZ010000099         6435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_14285_length_6435_cov_6.635423, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000099 DAOTHZ010000000
VERSION     DAOTHZ010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14285_length_6435_cov_6.635423"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="P5549_09345"
     CDS             complement(<1..1284)
                     /locus_tag="P5549_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRW13316.1"
                     /translation="MKKTTAVFAIMLILILAVSGIVPSIAAAPPQSLTILFTHDLHDN
                     LLPFYVADGDSIARRGGFARLQTVIDQQRALDPQLLLADAGDYSMGTLFQTIFAAEAP
                     GLRLMGQMGYDVTTLGNHEFDFRPQGLADSLQAARRSQEPLPQIVSANIAYPVDEHGK
                     IIGQDLQQLQQAMTDYGVKPYTILERKGYKIGVFGLMGNEAASNAPMAGVTFDDPLKK
                     ARQITDQLIQQEKVDLILCLSHSGTVEDPKKSEDVILAQKVPEIDVIISGHTHSRLEQ
                     PIVVGDTYICSADEYGQNLGMITLQPVSAGKWQQMSYRLLPIDDQVAADARINQRLTD
                     FKKRVQEHYLDRMEMQFDQTLAYIPFNYQDISAIGSQHAEQPLGNLISDAYLYAVKQA
                     EAEDYEPVAAAVVPYGTIRASFVKGDITVADAFNVS"
     gene            complement(1384..2328)
                     /locus_tag="P5549_09350"
     CDS             complement(1384..2328)
                     /locus_tag="P5549_09350"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRW13317.1"
                     /translation="MNRDHSEDQASINLSVNLGGIMMKNPVAVASGTFGYGFEYADFI
                     DSRTIGAVIVKGTTLEARPGNLPPRITETPAGMLNAIGLENPGVEVFLQEYLPALREQ
                     GVTVIANIAGNSLEDYAQMAARLEGHTGIAGIELNISCPNVKQGGLQFGTDPHMVQNV
                     VAAVKAESSLPVMPKLSPNVTDIVAIAKAAQAGGADALSMINTLLGMAIDVRTRRPVL
                     GNVFGGLSGPAIKPVALRMIYQVYREVNLPILGGGGIMNPTDALEFIMAGASAVSIGT
                     GIFVNPRLAADIAAGMEQYLRDNDLQDISQLVGAAVNR"
     gene            complement(2325..3125)
                     /locus_tag="P5549_09355"
     CDS             complement(2325..3125)
                     /locus_tag="P5549_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRW13318.1"
                     /translation="MATLEQALVISNEQMAADLYELEMIAPQVASLCKPGQFLQVRMD
                     VNHDPLLRRPISVYDVDQDRGTITLLYKVVGRGTNLLTTVKSQESLDIMGPLGNGFGL
                     PAQPVRAVLVGGGVGIAPLLYLARRLLAMQSEVTVLYGAGNRRELVAAQRFENIGAQV
                     LTATLDGSEGFQGLVTELLTAQIKPEETDCIYTCGPEIMMAAVAAFAVRHQIVGECSL
                     EENMACAVGACLGCARKLKSTDTHYVKVCKDGPVFSLQGIELYNGGVS"
     gene            complement(3142..6375)
                     /gene="carB"
                     /locus_tag="P5549_09360"
     CDS             complement(3142..6375)
                     /gene="carB"
                     /locus_tag="P5549_09360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRW13319.1"
                     /translation="MPLIEGLKKVMVIGSGPIIIGQAAEFDYAGTQACRALKEEGIEV
                     VLLNSNPATIMTDTNIADHVYMEPITIATVSKILDKEKPQGIIATLGGQVGLNMALAL
                     DREGILARTGVPLLGTPLEAIARAEDRELFKQTMEEIAEPVPESDIVTSVEAALAFAD
                     QIGYPVIVRPAYTLGGTGGGNAVNREELLRIAQKGIKSSPIGQILIERSVAGYKEIEF
                     EVMRDANDNCITICSMENVDPVGIHTGDSIVVAPAQTLTDEEYQMLRAASIKIIRALK
                     IAGGCNVQFALDPFSKKYFVIEVNPRVSRSSALASKATGYPIAKVTTKIAIGYHLDEI
                     PNVVTGKTTACFEPAIDYVVLKMPRWPFDKFVFGDRRLGTQMKATGEVMAIDRSFEAA
                     FLKSIRCLELGINGLRLPELADISDESLRNRLKEADDERIFMVAEAFGRSLSIDEVWE
                     ITRIDKYFLQKIKNIIDFTRRLSEQELDVELLREAKKTGFSDIEIAHLQEGLQEVHIH
                     RLRTDKGVIPTYKLVDTCAAEFDAVTPYFYSCYEDENEATHDPLAKKILVIGSGPIRI
                     GQGVEFDYCSVHCVWALREAGIKAIIVNNNPETVSTDFDTSDRLYFEPLVFEDVLNII
                     DEEKPDGVIVQFGGQTAINLAQPLNNVGVRIIGTSNDSIDRAEDRDRFDTLVEELGIP
                     RPPGKSVFTVEDAIRAAETIGYPVLLRPSYVLGGRAMEIIYNRTELEHYMATAVKVSR
                     EHPVLVDKYLLGTEIEVDAVCDGQQVLIPGIMQHIERAGVHSGDSMAVFPAYSLRPEI
                     IGKVVEYTTQLALALKVKGLINIQFVEFQQELYVLEVNPRSSRTVPFISKVTGIPLVK
                     LATEIMLGKNLPSLGYQSGLQPAPDFFAVKVPVFSFNKLEQVDITLGPEMKSTGEVMG
                     IDRSLKTALYKGLVAAGMVIPQKGTIVATIADKDKQEAIPILKGFYELGFKLIATQGT
                     AEALRQAGVEVESVRKMKEGSPHIADLIRQGKADFIVNTMTHGRDQFSDGFFIRRAAV
                     ELSVPCLTSLDTLKVMQEVIAEGEKIDDMEVKSLQEYVAGSLSPGQG"
BASE COUNT         1371 a         1856 c         1702 g         1506 t
ORIGIN      
        1 actgacattg aaggcatcgg ccaccgtaat atcgcctttg acaaaagaag cccggatcgt
       61 tccgtaggga actacggctg ccgcaacggg ttcgtagtcc tctgcttctg cctgcttcac
      121 cgcataaaga taggcatccg agattagatt gcccaaaggc tgctctgcat gctggctgcc
      181 aatggcggaa atatcctgat aattgaaggg aatgtaggcc agggtttgat caaactgcat
      241 ttccatgcgg tccaggtagt gttcctgcac ccgtttttta aaatcggtga gccgctgatt
      301 gatgcgggca tccgccgcga cctggtcatc gatggggagg aggcgatagc tcatttgctg
      361 ccacttgccg gcagagaccg gttgcagggt aatcatcccc agattctgac cgtattcatc
      421 cgcggaacaa atataggtat cccccaccac gatgggttgt tccaggcggc tgtgggtatg
      481 cccgctgatg atcacatcga tttccggaac tttttgggcc aggataacat cttccgattt
      541 tttggggtct tccaccgtcc ctgaatgaga caaacagaga atcagatcca ctttttcctg
      601 ctggatcagc tggtcggtga tctgccgtgc ctttttcagc ggatcgtcaa aggtcacccc
      661 ggccatgggt gcgttcgagg cggcttcgtt tcccatcaag ccaaatacgc cgattttgta
      721 tcctttgcgt tccagaatgg tatacggttt tacgccataa tcagtcatgg cctgctgcag
      781 ctgctgcaaa tcctgcccga tgattttacc atgctcatcc accggataag caatattggc
      841 gctgacaatc tgcggcaagg gctcctgact gcggcgggca gcctgcaaac tatccgccag
      901 accctgcgga cgaaaatcaa attcatgatt gcccagcgtg gtcacatcgt agcccatctg
      961 ccccatcaat ctcagtcccg gcgcttctgc cgcaaagatt gtctgaaaca aggttcccat
     1021 ggaataatct cccgcgtctg ccaacagcag ctgcggatca agcgcacgct gctgatcaat
     1081 caccgtctgc aggcgggcaa aaccgcccct tcgggcaatg ctgtcaccgt cggccacata
     1141 aaacggcagc aaattgtcat gcagatcatg ggtaaacaga atcgtgagcg attgcggcgg
     1201 cgccgctgca atcgaaggaa cgatcccgct gacagccagg atcaagatca gcatgatagc
     1261 gaagactgct gttgtttttt tcattctttc atcctcctgc ccctttttcc tctctcgtgg
     1321 tgataccatt ccggcaaaac atctggagcg ttgcctgttc cggagcatcg gcagcctgcc
     1381 cggttatctg ttcaccgctg cccccaccag ttgggatata tcctgtaaat cattgtcgcg
     1441 caggtattgt tccatcccgg cggcaatgtc cgcggccaga cggggattga caaagatgcc
     1501 ggtgccgatg gaaacggcgc tggcgccggc catgataaat tccagggcgt cggtgggatt
     1561 cataatcccc ccgccgccca gaatgggcag attgacttcg cggtaaacct gataaatcat
     1621 gcgcagggcc accggtttga tggccggtcc ggacaagccg ccaaacacat tgcccagcac
     1681 cggacggcgg gttctgacgt caatagccat gcccagcaag gtgttaatca tggacagcgc
     1741 atcagcgccg cctgcctggg ctgccttggc aatggcgacg atgtcggtca cattggggga
     1801 cagtttgggc atgaccggca gcgagctttc cgccttgacg gcagccacca cattttgcac
     1861 catgtgcggg tcggtaccga actgcagccc gccctgcttg acattgggac aggagatgtt
     1921 cagctcgatc cccgcgatgc cggtgtgacc ttccaggcgg gccgccattt gggcgtaatc
     1981 ttccagcgaa ttgccggcga tgttggcaat caccgtaacc ccttgttcac gcagggccgg
     2041 cagatattcc tgcaaaaaaa cttctacgcc ggggttttcc aggccgatgg cattgagcat
     2101 gccggccggg gtttcggtga tgcggggcgg caggttgccg ggacgggcct ccagggtggt
     2161 gcctttgacg atgactgcgc cgatggtgcg gctgtcgata aaatcagcat attcaaaccc
     2221 gtagccaaaa gtgccggagg ccactgccac cggatttttc atcatgatgc cgcccagatt
     2281 tacgctgaga ttgatgcttg cctggtcttc gctatgatcc ctgttcatga cacccctcca
     2341 ttgtatagct cgataccctg cagactaaaa acaggaccgt ccttgcagac cttgacgtaa
     2401 tgggtatcgg tactcttcag tttgcgggcg cagcccagac aggcccccac cgcacaagcc
     2461 atattttctt ccagcgagca ctctcccaca atctgatgcc ggacggcaaa agccgccacc
     2521 gctgccatca tgatttccgg cccgcaggta taaatgcaat cggtctcttc cggtttgatt
     2581 tgtgcggtca gcagctcggt caccaacccc tggaatcctt cgctgccatc cagggtggcg
     2641 gtcagtactt gcgcacctat attttcaaag cgctgggcgg ccaccagttc gcggcggttg
     2701 cctgctccat aaagcaccgt gacttcgctc tgcatggcca gcaagcggcg ggccagataa
     2761 agcaaggggg caatgcccac gcctccccct accaacacag cccgaacggg ctgggccggc
     2821 aagccgaagc cgttccccaa cggtcccatg atatccagag attcctggga tttgacggtg
     2881 gtcagcagat ttgtacctcg ccctaccact ttgtacagca gggtgatggt cccccgatcc
     2941 tggtcgacat cgtagacact gatcggccgt cgcagcagcg gatcatgatt gacatccatc
     3001 cgcacctgca aaaattgccc gggtttgcac aggcttgcga cctgcggcgc aatcatttcc
     3061 aattcataaa ggtcggcggc catctgctcg ttgctgatga ccagggcctg ctccagcgtt
     3121 gccattgctt ccactccttt atcatccttg tccgggcgat aacgacccgg ccacatattc
     3181 ctgcagtgat ttcacttcca tatcgtctat tttctctcct tcagcgatga cttcctgcat
     3241 gactttcaaa gtatccagcg aagtcagaca gggaacggag agctccactg cggcccggcg
     3301 gatgaaaaag ccgtcgctga actgatcgcg tccgtgggtc atggtattga cgataaagtc
     3361 ggctttcccc tggcgaataa gatcagcaat gtgaggagag ccttctttca ttttcctgac
     3421 cgattccact tccaccccgg cctggcgcag cgcttccgcg gtcccctggg tggcaatcaa
     3481 tttaaatccc agctcataaa agcctttcag aatcgggatg gcttcctgtt tgtccttgtc
     3541 ggcgatggta gcgacaatgg ttcccttttg cggaatgacc atccccgccg ccaccagccc
     3601 tttataaaga gcggttttta aagaacggtc gatgcccatg acttctccgg tggatttcat
     3661 ttccggtccc agggtaatgt caacctgctc cagtttattg aaggaaaaga ccggtacctt
     3721 gacggcgaaa aagtccggcg ccggctgcaa gccgctctga taacccaaag acggcaggtt
     3781 tttgcccaac atgatttcgg tggccagttt gaccagggga atcccggtca ccttgctgat
     3841 aaaaggcacc gtccgactgg aacgggggtt gacttccagc acatacagtt cctgctggaa
     3901 ttccacaaat tgaatattga tcaagccttt gactttcaag gccagggcca gttgggtggt
     3961 atactccacg actttgccaa tgatttccgg gcgcagggag tatgccggaa aaaccgccat
     4021 gctgtcgccg gaatgaactc cggctctttc aatgtgctgc atgatccccg gaatcagaac
     4081 ctgctggccg tcacagacgg catccacttc aatttccgta cccagcagat acttgtcgac
     4141 cagcaccgga tgttcccggc tgaccttgac ggcggtggcc atgtagtgtt ccagttccgt
     4201 ccggttgtaa atgatctcca tggcgcgccc gcccagcaca taggaaggac gcagcagcac
     4261 cggatagcca atggtttccg cggcccggat ggcatcttcg acggtgaata cgcttttgcc
     4321 cgggggacgg ggaatgccca gctcttccac cagggtatcg aagcgatcgc ggtcttcggc
     4381 gcgatcaatg ctgtcattgg aagtaccaat gatcctgacc cccacattgt tgagcggctg
     4441 ggccagattg atggcggtct gtccgccgaa ttgaacgata accccgtccg gtttttcctc
     4501 atctatgata ttgagtacat cttcgaagac cagcggttca aaatagagcc ggtcggaggt
     4561 atcaaagtca gtgctgaccg tttccggatt gttgttgacg ataatggctt tgatgccggc
     4621 ttcgcgcagc gcccagacac aatgcacgga acaatagtca aactccactc cctggccgat
     4681 gcggatcggg cccgagccga tgaccagaat ctttttggcc agcggatcat gggtggcttc
     4741 gttttcatct tcataacagg aataaaaata gggggtaacg gcatcaaact cagccgcgca
     4801 ggtgtcgacc agcttgtagg taggaatgac acccttgtcg gtgcgcagac ggtgaatatg
     4861 cacttcctgc agaccttcct gcaaatgggc gatttcaata tccgaaaagc cggtcttttt
     4921 ggcttctcgc agcagttcaa catccagttc ctgttcgctc aggcggcggg taaaatcaat
     4981 gatgttcttg attttctgga gaaaatattt gtcaatgcga gtgatctccc agacctcatc
     5041 gatgctcagg ctgcggccga aggcttccgc caccatgaag atgcgttcat catcggcctc
     5101 tttgagccga ttgcgcagtg attcgtcgga gatatccgcc aactcgggca ggcgcaggcc
     5161 gttgatgccc agttccaaac agcggatgga cttcaaaaag gccgcttcaa aactgcggtc
     5221 gatagccatg acctcaccgg tggctttcat ttgcgtaccc aggcggcggt cgccaaagac
     5281 aaacttgtcg aagggccagc gcggcatttt aagcaccaca taatcgatgg ccggctcgaa
     5341 acaggcggtg gttttaccgg tcaccacatt gggaatttcg tccagatgat agccaatggc
     5401 gatcttggtg gtcaccttgg cgatgggata accggtcgcc ttggaagcca gcgccgagga
     5461 gcggctgacc cgcgggttga cctcgatgac aaaatatttt ttactaaacg gatccagggc
     5521 gaactgcaca ttacagcccc ccgcaatctt gagcgcacgg atgattttaa tggaggccgc
     5581 ccgcagcatt tgatactctt catcggtcag cgtctgggca ggtgcgacaa cgatgctgtc
     5641 cccggtatga atccccaccg gatcaacgtt ttccatactg caaatggtga tgcagttgtc
     5701 gttggcgtcg cgcatgactt cgaattcgat ttccttgtaa ccggccacgc tgcgctcgat
     5761 gaggatctgt ccgatgggac tgctcttgat ccccttctgg gcaatccgga gcagttcttc
     5821 tctattcacg gcattgccgc cgccggttcc gcccagcgta taggccggac gaacgatcac
     5881 cgggtagccg atctgatcag caaaagccag cgcggcttcc accgaagtga cgatatcact
     5941 ctccggcacc ggttcagcaa tttcttccat ggtttgctta aacagttccc ggtcttcggc
     6001 gcgggcaatc gcttccagcg gcgttcccag cagcggcacc ccggtacgcg ccagaatgcc
     6061 ttcccgatcc agggccaggg ccatgttcag ccccacctgt ccgcccaggg tggcgataat
     6121 gccctgcggt ttttccttgt ccagaatctt gctgacggtg gcaatggtga tgggttccat
     6181 atagacatga tcagcgatgt tggtatcggt catgatggta gccggattgc tgtttaacag
     6241 caccacttcg atgccttcct ctttcaaggc ccggcaagcc tgggtgccgg catagtcaaa
     6301 ttcggccgcc tggccgatga tgatgggccc ggagccgatc accatcactt ttttaagtcc
     6361 ctctattaat ggcattcctt atcctccctt aataaacttt cattttcttt caatatctct
     6421 tgaaatcaca gtatt
//
LOCUS       DAOTHZ010000100         6402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_14378_length_6402_cov_7.180558, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000100 DAOTHZ010000000
VERSION     DAOTHZ010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14378_length_6402_cov_7.180558"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="P5549_09365"
     CDS             complement(<1..530)
                     /locus_tag="P5549_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13320.1"
                     /translation="MIYLINPIPGLFRKNKGFELEVSQVKYQSSRGQFHSVSAAEAVK
                     MGIAPDGGLFVPDSIPVLQSHDIAAMQQQDYQSRACQVMACYLDDFTAAEIADCVNAA
                     YNPAGFDHEGIAPLRQLEPNVFIQELWHGPTAAFKDMALQFLPHLMIYSVQKTQEESE
                     IVILVATSGDTGKAALA"
     gene            669..1214
                     /locus_tag="P5549_09370"
     CDS             669..1214
                     /locus_tag="P5549_09370"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HRW13321.1"
                     /translation="MGQFIAVIIILIIAKLIVDMVKDQLNRAKSSPKQGEVIDISDSW
                     VDTSTLPYQKKAQVMNPREITIYHSLARLLHDTDYIVVPHLHLSELIGVTDVPRQQEY
                     WQRLKERSVDLAILEAVTFQPVLVIHLLEEEVGRKQQLSNNFTTKAIKAAGLPQLDIN
                     PANPPVERELLAELRRQDLKI"
     gene            complement(1314..1637)
                     /locus_tag="P5549_09375"
     CDS             complement(1314..1637)
                     /locus_tag="P5549_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRW13322.1"
                     /translation="MNKSELVKALAEKAEITQKDAAKALDAVVETIQDALAKGDKVQI
                     IGFGSFEVRDRKARKVISPANGQEITVPATKVPAFKPGKSLKEAVAVKKAAKAPKAKK
                     AAKKK"
     gene            complement(2082..3197)
                     /locus_tag="P5549_09380"
     CDS             complement(2082..3197)
                     /locus_tag="P5549_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRW13323.1"
                     /translation="MPVTVREISRIMEALYPTDIAENWDNVGLQIGSMTGPVKSIMIT
                     LEADREIVTEAIRQHIDLIITHHPLFFKPLKTINFDHPQGELIKNLIRHNIHLYSAHT
                     NLDAGSAGLNQYLAEQLELQQIRLLNRSYSETLYKLVVYVPADHEAQVRESITAAGAG
                     HIGQYSHCTYRVAGTGTFLPGEGSQPFIGTPGQMEEVREYRLETIVPKNLLGKVLDSM
                     KQSHPYEEVAYDVYPLANQGTTYSPGRMGVLSRSMTLGDFCQHVKQKLAVDTLRIVGE
                     AEDTVQKVALVSGSGAGFIDQAHRQGCDVLLTGDLKYHEAKDAQALGLKIIDAGHQQM
                     ERLMAPLLAGQLRAACREKGCEITIVTCFSRECIQTI"
     gene            complement(3142..3882)
                     /locus_tag="P5549_09385"
     CDS             complement(3142..3882)
                     /locus_tag="P5549_09385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRW13324.1"
                     /translation="MEPLSPRLQAIVDLIGNGASMADIGSDHARLPLYLLQNGRISRA
                     IATELRDGPFRRTLAAVERCHLRQQIDVRQGDGLQTLAAGEVDVVVIAGLGGDSIAAI
                     LSHDWSKAERFPRYVFQPMTKADVLRKTLAARGWPILEEKLLLDNGRYVVILVSQPDC
                     TPYPLDEMEAEFGTEILQADDEIKRAYLFHFMQKYNKIYASLDKSARQDKEHWAKTCW
                     ERIQRLEMILDASYRPGNQQNNGSPLPD"
     gene            complement(3998..>4809)
                     /locus_tag="P5549_09390"
     CDS             complement(3998..>4809)
                     /locus_tag="P5549_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRW13325.1"
                     /translation="EEYLHRYAIEMLPLKVTFENDDTYLDRIEISPAQFVQKMQASRT
                     LPKTSAPDPATLVRCFEKGLEECGQVLFVSISSGLSSTYQTAQLASQMVNHPQVKLFD
                     TRTASLGTGIAAIQAADLAAQGRTLDEILDALEPLRERREVLFTLDTLENIVKGGRLS
                     KLQGMAATVLNIKPILRGNPQGVPEVAEKIMGRKKALQRLTDMLGEVYNRDLLRDKMI
                     GISHVSCPEDAHRLAAAIKQKYLPQPEIIVAEMGATIGSYAGVGGLMVSVY"
     assembly_gap    4810..4909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5026..5316
                     /locus_tag="P5549_09395"
     CDS             5026..5316
                     /locus_tag="P5549_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HRW13326.1"
                     /translation="MSEATLSLKILPVVSEEEIYPVVDQVIAMLANTGLKYVVSPNDT
                     TVEGDLNTLLELVKKAQQICIAAGARRVFSVVTIDYKPDGVTIDEKLSPYQS"
     gene            5288..6079
                     /locus_tag="P5549_09400"
     CDS             5288..6079
                     /locus_tag="P5549_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW13327.1"
                     /translation="MKNYPLTKAKLLPLATILFFLCLWEAGVRLTQTPAWILPSPGQV
                     LLALAEIGPALLVHTSTTLAEALLGFGLSVLIAFLLALLLDHFTGLRSALYPLIIFTQ
                     TIPLIVLTILFVIWFGFGMLPKVLVVILVCFFPVLINLMNGLDAVDHDQILLFKSMGA
                     KPGAIIWMVKIPTALPSFFSGLRISATYSIMGAIVAEWMGAEKGLGYFMTLAQKGFKV
                     DQVLAVVAVICVLSFLLVKLMDLLEYLLVPWNRPSTQPVYDEAVA"
     gene            6194..>6402
                     /locus_tag="P5549_09405"
     CDS             6194..>6402
                     /locus_tag="P5549_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW13328.1"
                     /translation="MQKFFGLLLVLVLSLSLAGCTDKSSSEPDALQKVTVLLDWVPNT
                     NHTGLYVALDQGYYQQAGLEVKLVQA"
BASE COUNT         1424 a         1593 c         1533 g         1752 t
ORIGIN      
        1 gccagggcag ccttgccggt atccccggaa gtcgccacca ggatcacaat ttcgctttcc
       61 tcctgggtct tttggacgct gtatatcatc agatgcggca ggaactgcag ggccatgtct
      121 ttaaaagcgg cggtgggccc gtgccacaat tcctgaataa atacgtttgg ctctaattgt
      181 cgtaacggcg ctatgccttc atggtcaaac cccgcaggat tgtaggccgc attgacacag
      241 tcggcaatct ccgccgcggt aaagtcatcc agataacagg ccatgacctg gcacgcccgg
      301 ctctgataat cctgctgctg catggctgca atgtcgtggg attgtaaaac aggaatagaa
      361 tctgggacaa acagtcctcc atcaggtgcg atgcccattt tcacagcttc tgctgcggaa
      421 accgaatgaa attggcctcg gctgctttgg tatttcactt ggctgacctc cagttcaaac
      481 cctttgtttt tccggaataa accggggatg ggatttatta aatatatcat atccgcatcc
      541 cttaattaaa tattattttc tctttgtgtc aacttaacat gcctgcaagg tttctcgtta
      601 ttaattgctg caggactggt ataataagaa tgatacatca caacagcttt taaccgggag
      661 gtatcgtaat gggacaattc atcgccgtga tcattatcct gatcatcgcc aaactgatcg
      721 tagacatggt taaagaccag ctgaaccgcg ccaaaagcag ccccaaacaa ggggaagtga
      781 tcgatatcag tgattcctgg gtagatacca gcactctgcc gtaccagaag aaagcccagg
      841 ttatgaaccc gcgggaaatt accatttatc acagcctggc ccggttgctg cacgacactg
      901 attatatcgt tgttccccat ctgcatctgt cggaactcat cggtgtgacg gatgtgcccc
      961 ggcaacagga atactggcag cgattaaaag agcgcagtgt tgatctcgcc attttggaag
     1021 ctgtaacttt ccaaccggtg ctggtaattc atttgctgga ggaagaggta ggccgcaaac
     1081 agcagttgtc caacaacttc accacgaaag ccatcaaagc cgccggcttg ccccagttgg
     1141 acatcaatcc cgccaacccc ccggtcgaac gagaactgct ggccgaactg cgccgccagg
     1201 acctcaagat ctaaaagtga attttgggga aaatccccaa aattcaccaa aaaaaagagg
     1261 tgtacgtgct tacacctctt ttcatttcca tttttatttc ctgttttctt ttcttatttc
     1321 tttttcgctg ctttcttggc tttgggagct ttcgctgctt tctttacagc cacagcttct
     1381 ttgagggatt tgcctggctt gaatgccgga actttggtag cgggaacggt aatctcttgg
     1441 ccattggcag ggcttatgac ttttctggcc ttgcggtcac gtacttcaaa actgccaaat
     1501 ccgataattt gaactttgtc gccttttgca agcgcatctt gaatggtttc taccactgca
     1561 tccaatgctt ttgcagcatc cttttgggta atttcagcct tttcagccaa tgcttttacg
     1621 agttccgatt tgttcacgac ataatcctcc cttaaaatat tttagtacta ttttctacat
     1681 tcatcataaa atcggcgatt cctgctaata aaccattatt ttctaaaatt tttttatttt
     1741 taggttattc tttgaaaaaa actctagttt cagtttccct gtttaaccag tttttataca
     1801 tggtttaatg cgaaaatccc aatcaaatgg gcacccggtc acacttcgat agaatcacct
     1861 cgcttgtcac aaactcttca ttgtttttta tttaaaacac tcagttgagc gggaaaaata
     1921 attgcgagat ttcgggccgg aatggttgta atttctttgc taacccaatg ataccttact
     1981 tttggttttg attccacaat aaatagaaaa acccttctat tttactgaaa ttttccgaca
     2041 tatttttcgc aaggcagtct tccctaaata ccgggtttgg gttagatggt ttgaatgcat
     2101 tcccggctga agcaggtgac aatcgttatt tcgcaacctt tttcgcggca ggcagcgcgc
     2161 aattgtcccg ccagcaatgg ggccatcagc cgttccatct gctggtgccc ggcatcaatg
     2221 attttcaaac caagagcctg ggcatctttg gcttcatggt attttaaatc tccggtcaag
     2281 agaacgtcac agccctggcg gtgagcctga tcaataaaac ctgctccaga gccgctgacc
     2341 agggcaactt tttgcacggt atcctctgcc tcgccaacga tccgcaaggt atcgactgcc
     2401 aatttttgtt tgacatgctg acagaagtcc cccaaggtca tggagcgaga caataccccc
     2461 attcggccgg ggctataggt cgtgccttgg ttggccagcg gatagacatc ataggcaact
     2521 tcctcgtagg gatggctttg cttcatgctg tccagcactt ttcccagcaa attcttgggg
     2581 acgatggttt ccaaacggta ttcgcggact tcctccattt gccccggcgt accgataaaa
     2641 ggctgactgc cttccccggg cagaaacgtt ccggtcccgg ccactcgata ggtgcaatgg
     2701 gaatattggc caatatgccc tgcgccggcg gcggtgatac tttcccgcac ctgcgcttca
     2761 tggtctgccg gcacatagac caccagtttg tacagcgttt cgctgtaact gcggtttaaa
     2821 agtctaatct gctgcaattc caattgctct gccaaatatt gattcagccc ggcgctgccg
     2881 gcatccaggt tggtgtgggc agagtaaaga tgaatgttgt gacggatcag gtttttaatc
     2941 agttcaccct ggggatgatc gaagttaatt gttttgaggg gtttgaaaaa caacggatgg
     3001 tgggtgatga tgagatcgat gtgctgccga atggcttctg tgacaatttc ccgatcggct
     3061 tcaagggtga tcatgatgct tttgaccggg ccggtcattg agccgatttg caagccgaca
     3121 ttgtcccagt tttcggcaat gtcagtcggg taaagggctt ccattattct gctgatttcc
     3181 cggacggtaa ctggcatcaa gaatcatctc caatcgttgt attcgctccc aacaggtttt
     3241 ggcccagtgc tctttgtcct gccgggccga tttatccaag cttgcataaa ttttattata
     3301 tttttgcata aaatgaaaca aatacgcacg ttttatttca tcatcggcct gcaatatttc
     3361 tgttccgaac tcggcttcca tttcgtcaag cggatagggg gtgcagtctg gttggctgac
     3421 cagaatcact acatagcggc cgttgtccag cagcagcttt tcctctaaaa tcggccaacc
     3481 cctggcagcc agcgtcttgc gcaatacgtc tgctttggtc atgggctgga aaacatagcg
     3541 aggaaagcgc tcggccttgc tccagtcatg ggagagaata gcggcaatgc tgtctccgcc
     3601 caggccggca attaccacca cgtcaacctc gcctgctgcc aaggtctgca gtccgtctcc
     3661 ctgacggaca tcgatttgct gccgcagatg gcagcgttca acggccgcca aggttcgccg
     3721 gaaaggtcca tccctcaatt cagtggcaat cgcgcgggat atccgaccgt tttggagcaa
     3781 atagagaggc aggcgggcat ggtcagaacc gatgtcggcc atgctggcac cgttgccaat
     3841 caaatctaca attgcctgca atctcgggct gaggggttcc atgacagcat cttctccttg
     3901 tgcgatggtg cttctctttc ttcaagcctg acaggaacag gcatagaaca atgaactgcc
     3961 tatttgacct gatacgggca tgaatggccg ggcggcgtta atacacactg accatcagtc
     4021 cccctacccc ggcatagctg ccgatggtcg cccccatttc cgcaacgatg atctccggct
     4081 gcggcaggta tttttgcttg atggcagcgg ctaaccggtg ggcatcttcc ggacaggata
     4141 catggctgat gccgatcatt ttgtctcgca gcaagtctcg attgtatacc tcgcccagca
     4201 tgtcggtcag tcgctgcaag gcttttttcc tgcccatgat cttttccgcc acttccggca
     4261 ctccctgggg gttgccccgc aggatgggtt tgatattcag cacagtcgcg gccattccct
     4321 gcagtttgct gagcctgccg cccttgacga tgttctccag cgtatcaagg gtaaacagta
     4381 cttcgcggcg ctctctcaat ggttcaagcg catccagaat ttcatccagg gttcgtccct
     4441 gcgctgccaa gtcggccgcc tggatggcgg caatcccggt tcccaacgat gctgtccggg
     4501 tgtcaaacaa ttttacctgg ggatgattga ccatttggct ggccagttga gctgtctgat
     4561 aggtgctgct caagcctgat gatatgctga caaacaagac ctgcccgcat tcttccagtc
     4621 ctttttcaaa acagcgcacc aatgtggccg gatcaggcgc cgaagtcttg ggcagggtac
     4681 gtgacgcttg cattttctga acaaattggg ccggacttat ttcgatacgg tccaaatatg
     4741 tatcatcatt ttcaaaggtg accttcaaag gcagcatctc gatggcgtat cgatgcaagt
     4801 attcctccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tacctgatct
     4921 gggtaatgcc agcgtaggga gcataattct tgtccgcttt gcttcttgct tttatcaggt
     4981 aagaagtttt tcgcttttct gatataaatg aaaggagttg gctttatgtc tgaagcaacg
     5041 ttgagtctca agattttgcc ggtagtcagc gaggaagaga tttatccggt ggtcgaccag
     5101 gtcattgcca tgctggccaa taccgggctg aaatacgtgg taagcccgaa tgatacaacg
     5161 gttgaaggcg atctgaatac cctcctcgaa ctggttaaaa aagcccagca gatttgtatt
     5221 gctgccggtg cccggcgggt tttttccgtg gtgaccatcg attataaacc agacggagtg
     5281 acgattgatg aaaaattatc cccttaccaa agctaaactg ttgccgctgg caaccatcct
     5341 gttcttttta tgcttgtggg aagcaggggt acggttgacc caaacgcctg cctggatctt
     5401 gccttcgccc ggccaggttt tgctggccct ggctgagatc ggaccagctt tgctggtcca
     5461 tacctcaacg actctggcag aggcattgct gggatttgga ctgtctgtcc ttatcgcttt
     5521 tcttctggcc ttgttgctgg atcatttcac cgggctaagg agcgcgctgt atcctttgat
     5581 catctttacc cagacgatcc ccttgatcgt actgaccata ttgtttgtaa tctggttcgg
     5641 gtttggcatg ctgccgaaag tattggtagt catcctggtt tgtttttttc ccgtcctgat
     5701 caatttgatg aatggattgg acgccgttga ccatgaccag attttattgt ttaaatccat
     5761 gggcgccaaa ccgggtgcca tcatctggat ggtcaagatc ccgacagccc tgccttcctt
     5821 cttttccggg ctgagaatat cagccaccta cagcattatg ggagccatcg tggcagaatg
     5881 gatgggcgcg gagaaagggc ttggttattt tatgaccctg gcacagaaag gcttcaaggt
     5941 tgatcaggtg ctggcggtgg tggcagtgat ttgtgtgctc agttttttgc tggtcaaatt
     6001 gatggatctg ctggaatatc tcctggtccc ctggaatcgg ccttcgaccc agcccgtgta
     6061 tgatgaagcg gtcgcctgag caaacgttca attttgtgcc tggcatcatt gataaaatcc
     6121 tgccctgcgg ttacgcagcg cgaaaacaag tatccttcag caaaatattt actttggaga
     6181 ggagacgtta tcattgcaaa aattctttgg actgttattg gttttggttc tgtcattgtc
     6241 cctggctggc tgcacagaca aatcttcgtc cgagccggat gcgctgcaga aagtgacggt
     6301 attgttggat tgggtgccca acaccaatca taccggactg tatgtcgcgc tcgatcaagg
     6361 ctactatcag caagccggat tggaggtcaa gctggtccag gc
//
LOCUS       DAOTHZ010000101         6195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_15012_length_6195_cov_6.106840, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000101 DAOTHZ010000000
VERSION     DAOTHZ010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15012_length_6195_cov_6.106840"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..619
                     /locus_tag="P5549_09410"
     CDS             137..619
                     /locus_tag="P5549_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HRW13329.1"
                     /translation="MNLTDQEIIALLQYSFVRLDGAWFLSLAGSHGTETAWNTDVDVW
                     KHFSYLFGKRIRSEHIPEPVWPESYIEAMEILAKVLKIEGREVTLEPDRIIIRVTDCE
                     TQKAIAKANVADCGIATIQTYQGLARGLFNKELEVAVEHTKNLNHGDPFCEVVIRREG
                     "
     gene            complement(848..2467)
                     /locus_tag="P5549_09415"
     CDS             complement(848..2467)
                     /locus_tag="P5549_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRW13330.1"
                     /translation="MKNKLLTRIKQINQKIKESGDKTVPLDIRLFLQLSVLVLTIIAG
                     VVAILFITGTFTAGLDESQHLVENELDHAASAITQQYGELSVQAIDFSLELSRSIEAK
                     TNQKGIPLSDLEQHPEILEEIISGEFDRAMFTLQRAKSSGVFFILNATINPALANAEN
                     SRAGLYIKNMEPNIINSSSPNIIVFRGIPKIGRDHALSLHTQWKMEFDISDASYYHRP
                     FQAGAIHTDLPLSRLYYWNPVTLLPDSSEEAMLCSVPLIDSRGNVFGVCGIEISTMLF
                     KLTNMPDSDPFKRLFCVLSPVNENTLDLQHSMFAGGYSAKIISKGHDRLNISKERRSF
                     YSYSHQDHSFLGVHKPVQLYPQGSAFENEKWVAAIMIPEDDIVTSLTRLNLMLIALLM
                     LLVVLGVLVSLILSRKFLQPITQGLDMIKTTDLSNAPSTKIAEIDDLIKYLSEHNQEL
                     SERAQQENLSINLLDEFLAKIKELSPAERAVFDLYVEGHTAKEAAEKLCLSINTIKTH
                     NKKIYMKLNVASRDELLLYISMLKEIGAEIE"
     gene            complement(2479..3864)
                     /locus_tag="P5549_09420"
     CDS             complement(2479..3864)
                     /locus_tag="P5549_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRW13331.1"
                     /translation="MKKKILFVILGLMLITILAGCSNAKNVNTNQPVTLTLWHNYGGQ
                     LKDTMDEMVDQFNETTGAEQGIIINVTSISGSAALHEKLIMAANGDPGAPTLPDITTA
                     YPKTALILAEKDLLADLDQQFTPEELEAYIPAFIEEGRLNGQGLYVFPTAKSTEVLFV
                     NQTIFDRFAKDTGAKLEDLKTFEGLLETAALYYEWTDRQTPDILHDGKTFFMADSLFN
                     AALIGCKQLGQELVADDTLKTDTPAYQKIWDTYYKSAVLGHTAIYDGYATDLAKTGDI
                     VCSTGSTAGVSFFSPTVTYADNTAEPAELAILPYPVFENGRPTALQRGAGMCVTKSTA
                     EKEHAAAVFLKWFTSPENNLHFVVSTGYMPVTQAAFGELMTKELENIDDRNIRKLSQA
                     IRTMQEEYQFYTTPLFTGIDELQEQYEAQLKETAVQSRSSYCKYLPGSDIQTAYDAAS
                     LGKLEEFSDSF"
     gene            4095..4427
                     /locus_tag="P5549_09425"
     CDS             4095..4427
                     /locus_tag="P5549_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13332.1"
                     /translation="MTEPNALPVIDQADIDQNKTMAGLAYIIFFLPLLACPDSQFGKF
                     HANQGLLLLILGFAGSFILSIIPIIGWIILPFFGIGVFVLAVIGLINGFGGKVKELPL
                     IGHFRLIK"
     gene            4468..4977
                     /locus_tag="P5549_09430"
     CDS             4468..4977
                     /locus_tag="P5549_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13333.1"
                     /translation="MFKKIGIVLLAAALLLTLTSCGVKESVNDKIAEKVTEGVMNKAT
                     GGDANIDIDGDKITVEGKDGEKVNIGDSEWPAVLADNQIPEFKSGKILSSINVDNGCM
                     VMMEEVESEDFKQYLADIKAAGYTNDATELNSPESQIYSAYLNENTMVYLQYAPESKT
                     LNVSLEVSE"
     gene            5080..5916
                     /locus_tag="P5549_09435"
     CDS             5080..5916
                     /locus_tag="P5549_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13334.1"
                     /translation="MKLKLLVMSLFLCMTLMVSGCGGSSDSEPAGGEPAAPAAAGDKA
                     EQQSSSDFKQISDFADAWNAAYEQNEAAINSYEVPIMELVTPGTEFITGVQYDLLNME
                     NKDGRFEGELMMAGFPGFVEKNGSKITFGYDYTRDEDGFSPSMKAGDRLIENGSCDLA
                     AEIYQTESYTERNGEKVQRSYSEFKRLKNQDMICLVTNGSSFNLREEADPANTCIYIK
                     AGKDKFDFVIGKATVGPAFEKLSFVDKSDLTKDDARKLMEAAGYQIEQTGGISGGTLV
                     VD"
BASE COUNT         1597 a         1395 c         1566 g         1637 t
ORIGIN      
        1 atcaccagat catactgaca ggtactatca aaaacgttaa aatgcatata cataaacaga
       61 ctgaagggtt tatttgggag ctatacttag cgccttaaaa tactgaggat tctattaggg
      121 agatggaggg agagatatga acctgaccga tcaggagatc attgctttgc tgcaatattc
      181 gtttgtgcgc ctggatgggg cctggttcct gtcactggct ggatcacacg gaacggagac
      241 ggcctggaac acagatgtgg atgtatggaa acatttttcc tatctgttcg gcaaaagaat
      301 caggagtgaa cacataccgg agccggtttg gccggagagt tatatcgaag ccatggagat
      361 cctggccaaa gtattaaaga tcgaaggccg ggaggtcacc ctggaaccgg accgcattat
      421 catccgggtc acggactgcg aaacgcagaa ggccattgcc aaagccaatg tagctgattg
      481 cgggatcgcc accatccaga cctatcaggg actggccagg ggcctgttta acaaggaact
      541 agaagttgcg gtggagcata ccaaaaactt aaatcacgga gatcctttct gtgaagtagt
      601 gatcaggcgt gaggggtgag gagaagcttc gctcgacgtc gtaggggccg acgaccctgt
      661 cagccctttg actatgtggt ttaacaatcg ggtggacaga gtcgtccacc cctacagcgc
      721 acgagcaact ctctcctgtc cccgatcccc tgtctactga ccaactgtct ccttttccct
      781 agcctctaac caactaacca actaaccaac tagcccctag tccttagccc ctagccccta
      841 acctgcttca ctcaatctcg gctccgatct ctttcagcat actgatgtac agcagcaatt
      901 catcccggga ggctacattt aatttcatat agattttttt gttgtgggtc ttgatggtgt
      961 tgatgctcaa gcataatttt tcagcggctt ctttggcggt atggccttcc acatagaggt
     1021 cgaatacggc ccgttctgcc ggggacagtt ctttgatttt ggccaggaat tcatccagca
     1081 ggttaatgga caggttttcc tgttgggctc tttccgacag ttcctggtta tgttcggaca
     1141 aatatttgat caaatcgtcg atctcggcta ttttggtact gggcgcgttg ctcaagtcgg
     1201 tggttttgat catatccagg ccctgggtaa tgggctgcag aaattttctg cttaagatca
     1261 gggaaaccag tacccccaga acaaccagta acatcagcaa ggcaatcagc attaagttta
     1321 gtctggtaag ggatgtgact atatcgtctt cggggatcat aatggctgcc acccattttt
     1381 cattttcaaa agctgaacct tgcggatata gctgtaccgg tttatggact cccaaaaaag
     1441 aatggtcctg atgactatag gaatagaaac tgcggcgttc cttcgagata ttaagccggt
     1501 catgcccttt ggagatgatt ttggccgagt agccgccggc aaacatggaa tgctgcaaat
     1561 ccagggtatt ttcattgacc ggggaaagca cacagaatag ccgtttaaat gggtcgctat
     1621 ccggcatatt ggttaatttg aaaagcatgg tgcttatttc aatcccgcag acaccgaaga
     1681 catttcccct ggaatcaatc aggggcacgg agcacagcat ggcttcctca ctgctgtcgg
     1741 gcagtaatgt aaccggattc caatagtaca ggcgcgacaa aggcagatca gtatggatcg
     1801 caccagcctg aaaaggccgg tggtaatagg aggcatcgct gatgtcaaac tccatcttcc
     1861 actgggtgtg caaagataag gcatgatcgc ggccgatttt gggaataccg cgaaaaacaa
     1921 tgatattggg cgaagaggag tttataatat taggctccat gtttttaata tataacccgg
     1981 ctctggagtt ttcagcattg gcaagggctg gattgatggt ggcatttaat atgaaaaaga
     2041 ccccgctgct cttggctctt tgcagcgtaa acatggcccg gtcaaattct ccggaaataa
     2101 tttcttccag aatctcagga tgctgctcca gatcagataa ggggatcccc ttttggttgg
     2161 ttttggcttc aatgctgcgt gataattcca gggagaaatc gatggcctgg accgacagtt
     2221 ccccatattg ctgcgtaatg gccgaggcgg catggtccag ttcgttctcc actaggtgct
     2281 ggctttcatc gaggccggcg gtgaaggttc cggtgataaa aagaatggcc accacgccgg
     2341 caatgatggt gagaaccaat acggaaagct gcagaaacag cctgatgtcc agcggaacgg
     2401 ttttatcgcc ggattccttt attttctggt ttatttgttt aatccttgtc agcagtttgt
     2461 ttttcaattc cattgcctct aaaacgaatc actgaactct tccagtttcc ccaatgatgc
     2521 ggcatcataa gctgtctgta tgtcgctgcc gggcagatac ttgcaatagg aagatctgga
     2581 ttggactgcg gtttccttca gctgtgcttc atattgctcc tgtaattcgt cgatcccggt
     2641 aaacagcgga gtggtataaa attggtattc ttcctgcatg gttctgatcg cctgggagag
     2701 cttccttata ttgcgatcat cgatattttc cagctctttt gtcatcagct caccgaaagc
     2761 ggcctgggtt accggcatgt acccggtgga aaccacaaaa tgcagattgt tttccggact
     2821 ggtgaaccat tttaagaaaa cggcggcggc atgttctttt tccgcagtgg atttggtcac
     2881 acacatcccc gctcctctct gcagagctgt aggccggccg ttttcaaaca ccggataagg
     2941 cagtatggca agctctgccg gttcagcagt attgtctgca taggtgacag tgggtgaaaa
     3001 gaaagagacc ccggcagtgg aaccggtgga acagacgatg tccccggttt tggccaggtc
     3061 ggtggcatat ccgtcataaa tagcggtgtg ccccaggacg gcacttttat aatatgtatc
     3121 ccatatcttt tggtaggcag gtgtatccgt tttaagggtg tcatctgcaa ccagttcctg
     3181 tcccagctgc ttacagccga tcagggccgc attaaagagt gaatcggcca tgaaaaaggt
     3241 tttgccgtca tgtaatatat ccggagtctg ccggtcagtc cattcataat aaagggcggc
     3301 ggtttcaagc agaccttcga aggtcttcag atcctccagt ttggccccgg tatccttagc
     3361 aaagcggtca aagatggtct ggttgacaaa taacacttcc gttgatttgg cggtgggaaa
     3421 tacataaagc ccctgcccgt ttagccgccc ttcttctatg aaggcgggga tataagcctc
     3481 cagttcctcg ggggtaaatt gctggtcaag atccgccagc aggtcctttt ccgccaggat
     3541 cagtgctgtt tttggatagg cagtggtgat atccggcaga gttggtgccc cgggatcacc
     3601 gttggcggcc atgatcaatt tttcgtgcag ggctgccgag ccggaaatgg aagtcacatt
     3661 gataatgatg ccctgctctg ccccggtggt ctcattgaat tgatccacca tttcatccat
     3721 ggtatctttt aactgcccgc catagttatg ccagagtgtt aacgttacgg gttggtttgt
     3781 gtttacattt tttgcattgc tgcagcctgc caggatcgtg ataagcatca gacccaggat
     3841 cacaaaaaga attttcttct tcaatttact cccgcccctt attcaactgt taaaaatatt
     3901 atttatgcca ttcagatact tgtccccaaa attgtattaa ttataacatt tttcactaaa
     3961 atataggact tatttcatca ttttacatca ttctgagggt gattaattgc agaatcaccc
     4021 ctggggtgat tactttttat ctggtaatta atacaataaa gttgaaaaat aattttttta
     4081 aggaggattt atgaatgact gaaccaaatg ctcttccggt aattgatcaa gctgacattg
     4141 atcaaaacaa aaccatggcc ggcctggcct atatcatttt tttccttccc ttgctggctt
     4201 gtcccgattc tcaatttgga aaatttcatg caaatcaggg tttgttattg ctgatcctgg
     4261 gttttgcagg tagtttcatt ttgagcatca ttcccatcat cggttggatc atcctgccct
     4321 tttttgggat cggcgttttt gttctggcag tgattggcct gatcaacgga tttggaggca
     4381 aagtaaagga acttcctttg attggacatt tccggctgat aaaataatcg atcctttcat
     4441 tgttaaaaac aaaaaggggg ttgaattgtg tttaagaaaa ttggaatcgt gctgctggca
     4501 gcagcactgc tgctgacttt gacatcctgt ggtgtgaaag aaagcgtgaa tgacaaaatc
     4561 gccgaaaagg tcaccgaggg cgtaatgaac aaagccaccg gcggtgacgc caatatagat
     4621 atcgacggag acaagatcac tgtggagggc aaagatggcg aaaaggtgaa cattggcgat
     4681 agtgaatggc cggctgtcct tgccgacaat caaataccgg aatttaaatc cggcaagatc
     4741 ttatcctcga taaacgttga caatggttgt atggtaatga tggaagaggt cgagtcagag
     4801 gatttcaagc agtatcttgc agatatcaaa gctgccggtt ataccaatga tgccactgaa
     4861 ctcaacagcc ctgagagtca aatatactcg gcctatttaa atgaaaacac catggtctat
     4921 ttacagtatg ccccggaaag taaaaccttg aacgtcagcc tggaagtcag tgaatagttg
     4981 attattacta aaggggactg gggataaaag gggacgcagg gttgtcgcgg cagccctcgt
     5041 ccccaatcat tacattcatt aattgggggg ttgtagatta tgaaattaaa gttactggta
     5101 atgagtcttt ttttatgcat gacactgatg gtcagtggct gcggaggcag ttccgattcc
     5161 gaaccggccg ggggtgaacc tgctgctcct gccgctgccg gggacaaggc tgagcagcag
     5221 tcttcatcag atttcaagca aatcagtgat tttgctgatg cctggaatgc tgcatacgaa
     5281 caaaatgaag cggcaatcaa tagttacgaa gttccaatca tggagctggt tacgcccggt
     5341 actgaattta tcaccggggt gcaatatgat ctgctgaata tggaaaacaa ggacggccgt
     5401 ttcgaaggcg aattaatgat ggctggcttt cccgggtttg tggaaaaaaa cggctctaaa
     5461 atcacctttg gttatgacta tacaagggac gaggatggtt tttccccatc gatgaaggcc
     5521 ggggaccgtc tgatagaaaa cggcagttgt gacctggccg ctgaaatata tcaaaccgag
     5581 tcttatacgg agcgcaacgg agaaaaggtc cagcgaagct acagcgagtt taaacggctc
     5641 aaaaaccaag atatgatttg tctggtaacc aacggctcca gttttaattt gcgggaagaa
     5701 gcagacccgg ccaacacctg catctatatt aaagccggca aagacaagtt tgacttcgtt
     5761 atcggcaaag ctaccgtggg gccggctttt gagaaactat cctttgtaga caaaagcgac
     5821 ttgaccaaag atgacgcgcg caaactgatg gaagcagccg gatatcaaat cgaacaaacc
     5881 ggcggcatct caggcggcac cctggtggtt gactaaatgt tgtcaggggt gaatcaaggg
     5941 gacagctggt acggagtgag gaggtagtgg tcgacgactc tgtcgacccg ctgcctggta
     6001 gacaatatcg ctcaaacgtt gtaggggtcg acgactctgt cgacccgctg cctggtagac
     6061 aatatcgctc aaacgctgta ggggccgacg accctgtcga cccgctgcct ggtagacaat
     6121 atcgatcaaa cgttgtagga gccgacgacc ctgtcggccc gttgcctggc agacaatatc
     6181 gctcaaacgt tgtag
//
LOCUS       DAOTHZ010000102         6038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_15523_length_6038_cov_7.984957, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000102 DAOTHZ010000000
VERSION     DAOTHZ010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15523_length_6038_cov_7.984957"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="P5549_09440"
     CDS             <1..1086
                     /locus_tag="P5549_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HRW13335.1"
                     /translation="VTTAIGYDLEGNNRLRFSSLYTVPSLPGETSSVSQAKPVLTISS
                     DYSGAMAARRTMLSLSKVPEYAHIRSIVLGDNLVKNNLPLMVDLLTRNRNFSPNTSLL
                     VSVDSHPEEILALVNDSDQSLQMMVVFNELQLGIYVPVTIYDFIFKLLTPGIEPVMPQ
                     ITIEAKKPDNQSASFSDQVESLAAGKKKLALHHTAVFKKDKKVGFLNEYESRGLRWLN
                     SRTKTGGVIVIKSPSDASAYTALEILCFHSNTRALVDGGQIKMQINIKARLAMNENTG
                     GVNNITKDVLGDMEQTANQEIARQIKSCIRKSQSLNSDVLGWGLLLLQYEPDTWKQVK
                     GDWNYIYPQIPYEVTAETSIIHTYLSK"
     gene            1111..1359
                     /locus_tag="P5549_09445"
     CDS             1111..1359
                     /locus_tag="P5549_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13336.1"
                     /translation="MPPRLIYSLLLFSMLYVLLEARPLFKTRAYKDLAVTSLILILAL
                     SYGIDYMMGTQLLPNPNRLIILFKPLSEALNSFFQVNT"
     gene            1374..>2456
                     /locus_tag="P5549_09450"
     CDS             1374..>2456
                     /locus_tag="P5549_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HRW13337.1"
                     /translation="MPEKVLSRQQQASMIFLLLIGSSLVFLTGSAASQNAWLSTCAGS
                     FFGLYVLYAIISIHNLFPGQRITQVSTQVLGSVPGTLLNGLFLGSVFAILVTFLFDII
                     MMMEIIYPLVPRVVLYPLLVLPCCYCLYKGITVLGRLGELILAITMIIVLLGMLLSLP
                     NIEISNLQPILANWRPFVAGTLYAADWPFDEVIIFALFLPLVSDYRRNQNIYYYGYLI
                     GALLLTLIDVTIITVLGESQANLSLFPLFETSRLLGFENFQRVELLFFVIWLFTGLYA
                     MVIYFQGLAFVIQDVFSLKSNKSLILPLGLCIVVFTLYMFPNTVEYTLLGFSYLPVYT
                     FPVNLFYPTIILCVAKYRQKHPSQKLL"
     assembly_gap    2457..2556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2578..3714
                     /locus_tag="P5549_09455"
     CDS             2578..3714
                     /locus_tag="P5549_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRW13338.1"
                     /translation="MLLVAFLLGGALIAHQQGTSLWAAFTDSSPQQAAIPSETTTAAA
                     LTIPTEAVSIADMVEIASPAVVNIKSTVKVSSVYDNPFFSDPFFREFFGPQSFNTIPQ
                     YETGIGTGFLITADGYILTNQHVIEDATKVTVQLNGKSEEIPAQMISSDRELDLAVLK
                     ISGSNYPVLPLGNSDQMRVGEWVVAIGQPYGLDHTVTTGVISAKGRPISIEDRQYKNL
                     IQTDAAINPGNSGGPLLNLKGEVIAINTAVNAQAQGIGFAIPINTVQEVLQDLISGNK
                     IVRPYLGIRMADLNDQLRSELNVTSSIKGTVIVEVVSGSPAAAAGLSSKDIITSLNDT
                     PVESASGLQDLIAAHKVGDKVSLNVIRSGRQMTLNATLQAKPNA"
     gene            complement(3753..>6038)
                     /gene="mfd"
                     /locus_tag="P5549_09460"
     CDS             complement(3753..>6038)
                     /gene="mfd"
                     /locus_tag="P5549_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRW13339.1"
                     /translation="QVGFYKHIAQRDMEPFHNQLENLYPRLQEWLAKDYEVVLAIKKK
                     NLQEEMAGQLLDHHVSGVKFTSLPYSRGFISPDMGVALISEKDLWPKKNFSGKKVKRS
                     AEQRLLIEDLKLGDYVVHENYGIGIYRGVTQVQSDGVTREYLLLQYAGTDKLYLPVDK
                     LELLFRYSSSEDKSPRLSKLGGTEWERTRKRVADSIQDMAEDLLRLYATRESVQGFAF
                     SPDTPWQRQFEDDFPYQETPDQLKAIEDTKRDMEDSKPMDRIVCGDVGYGKTEVALRA
                     AFKAIMDGKQVAILVPTTVLAEQHYQTFQQRFQNYPANIEVLSRFRTTAQQKKIVQAI
                     NQGGVDIVIATHRLLSKDIMFANLGLLVIDEEHRFGVAQKEKIKSLKAQVDVLSLSAT
                     PIPRSLHMGLTGLRDLSVIETPPPQRYPITTYVMEYNEEIVKEAVMTEFARGGQIFYV
                     HNRIQDIQKVKAQLDKLLPQVQIAVGHGRMREEELARTIMEFIEGKFDLFLCTTIIES
                     GLDMPNVNTIIVDMADHMGMAQLYQLRGRVGRSDRVAYAYLTYRPDKAVTEIAQKRLN
                     AIREFTELGAGMKIALRDLEIRGAGNILGAEQHGHIYAVGFDLYCRLLEQETAKLRGI
                     VATETVNPQLDIDVDYYIPDTYIPDPGTKMRIYRQLLLAGAAEEIEDIRNEIEDRFGR
                     LPTAVENFMQIAALRIKARDKAIKFLHRKGDKIEIVLNQPLENNIKVPGLKKVNPYTL
                     NIQMEKGSSLTALREIVELIQ"
BASE COUNT         1457 a         1493 c         1336 g         1652 t
ORIGIN      
        1 gttacgacgg ccattggcta tgatttggag ggaaacaacc ggctgcgttt ttccagtctg
       61 tataccgttc cttctttacc gggagaaaca tccagtgtca gccaggccaa acccgtctta
      121 accatcagca gcgattacag cggcgctatg gccgcccgca gaaccatgct gtctttatcc
      181 aaagtgcctg aatatgcgca tattcgcagt attgttctgg gagacaattt ggttaaaaac
      241 aatctccccc tgatggttga cttattgacc cgcaatcgta atttttcccc caataccagc
      301 ctgctcgtca gtgtagacag tcatccggaa gaaatattgg cattggtcaa tgattcggat
      361 caaagtttac aaatgatggt tgtctttaat gaattacaac tgggcattta tgttccggta
      421 acgatttatg attttatctt taaactgctt actccgggaa ttgaaccggt catgccgcaa
      481 attacaattg aagctaaaaa acctgataat caatctgctt catttagtga ccaggtggag
      541 agccttgccg ccggcaagaa aaaactggca ttacatcata ccgcggtctt taaaaaagac
      601 aaaaaagtcg gctttctaaa tgaatatgaa agccgcggct tgcgatggct gaactcccgt
      661 accaaaactg gcggagtgat cgtaataaaa tccccttctg atgcttccgc ttataccgct
      721 ttggaaatcc tctgtttcca tagcaatacc cgggcgctgg tggatggcgg ccagatcaaa
      781 atgcagatta atataaaagc tcgtttggcc atgaatgaaa ataccggcgg agtaaataat
      841 atcaccaagg acgtccttgg tgatatggaa caaaccgcca atcaggagat cgccaggcaa
      901 atcaaaagct gtattcgcaa atcgcaaagc ctcaacagtg atgtattggg gtggggcctg
      961 ctcctgctgc aatacgaacc ggatacctgg aagcaggtca aaggagattg gaattatata
     1021 tatccccaga taccttatga agtcaccgct gagacttcga ttattcatac gtatttgagc
     1081 aaataagtgc tctttaggaa ggtgaaatga ttgccgcccc gccttatcta ttcattgtta
     1141 ttgttttcga tgctgtatgt cctgctggaa gccagaccac ttttcaaaac gcgggcttac
     1201 aaggatctgg cggtcaccag tctaattctg atcttggcct tgagctatgg aatcgattac
     1261 atgatgggaa cgcaactcct gcccaatccc aatcgtctga taatcctgtt caaacccttg
     1321 tcggaggcat taaacagctt ttttcaggtg aatacgtaag cggaggttaa aaaatgccgg
     1381 aaaaggtttt atcccgccag caacaggcaa gcatgatttt tttattgtta atcggcagtt
     1441 cgctggtttt ccttaccggc agcgcagcat cgcaaaatgc ctggttgtca acctgcgcag
     1501 gctctttttt tggtctgtat gtactctacg ccattatcag tatccataac ctgtttcctg
     1561 gacaaagaat cactcaggtg agcacacagg tactgggcag cgttcccggg actttattga
     1621 atggcttgtt tttggggagt gtctttgcca tcctggtgac gtttctgttt gatattatta
     1681 tgatgatgga aatcatttat cccttggtgc cccgggtcgt tttgtatccg ttgctggttc
     1741 tgccctgctg ctactgtctt tataaaggaa tcactgtttt ggggcgtctg ggggaactca
     1801 tcctggcaat aacaatgatc atcgtcttgc tggggatgct gttgtccctg ccgaacattg
     1861 aaatatccaa cttacaacct attctggcaa actggcgccc tttcgtcgcc ggaacattgt
     1921 atgcagctga ctggcccttt gatgaggtca tcatctttgc cctgttttta ccgttggttt
     1981 ctgattatcg gcgcaaccaa aacatctatt actacgggta cttgattggc gccttattgc
     2041 tcactctgat tgatgtcacg atcatcaccg tattaggtga aagtcaggca aatctttcgc
     2101 tttttcctct ctttgagact tcacgtttgt tgggatttga aaattttcag cgagtggaac
     2161 tcctgttttt tgttatctgg ttgttcacgg gattgtatgc catggttatt tattttcagg
     2221 gtcttgcctt tgtcattcag gacgttttca gcttaaaaag taataaatca ttaatcttgc
     2281 cgctgggatt atgtatcgtg gtttttacgc tgtatatgtt ccccaatacg gttgaatata
     2341 cgttactggg atttagttac cttccggttt atacttttcc tgtcaatctt ttctatccga
     2401 ccatcatcct ttgcgtggcc aaatatcggc aaaagcaccc ttcccaaaag ttgctgnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat tcagcaaaac cattattctg
     2581 ctgctggttg cgtttctgtt gggcggagcc ttgattgccc accagcaggg cacatctttg
     2641 tgggcagcat ttaccgatag ctcgccccag caggcagcaa tcccatcgga aacgaccact
     2701 gccgctgctt tgacgatccc gacagaagct gtcagcatcg ctgatatggt ggaaatcgcc
     2761 agtccggcag tcgtcaacat caaatccacg gttaaagtca gctccgtcta cgacaatcct
     2821 tttttcagtg atccgttttt tcgcgaattt tttggtcctc aatcctttaa caccattccc
     2881 cagtatgaaa ctgggattgg gacaggtttt ttaattactg ccgacgggta catcctaacc
     2941 aatcaacacg taattgaaga cgccaccaaa gttaccgtac aattaaacgg caaatccgaa
     3001 gaaattccgg cccagatgat cagttctgac cgtgaactgg acctggcggt gctgaaaatc
     3061 agcggcagca actatccggt tctgcctttg ggcaattctg accaaatgcg ggtaggagaa
     3121 tgggttgtgg ccatcggcca gccttatgga cttgatcaca ccgtaaccac cggagtcatt
     3181 tccgccaaag ggcgtccaat tagcattgaa gatcgtcaat acaaaaacct tatccagacg
     3241 gatgcagcga tcaaccccgg caactcaggc ggtccgctgc taaacctgaa aggtgaagtc
     3301 attgccatca atacagcggt caatgcacag gcccaaggga tcggttttgc catccccatc
     3361 aacaccgtac aagaagtgct gcaggacttg attagcggca acaagattgt cagaccgtat
     3421 ttgggaatcc gaatggctga tttgaatgac cagctccgtt ccgaactgaa tgtgaccagt
     3481 tccatcaaag gtacggtaat tgtcgaagtt gtcagtggct ctcctgctgc cgcagccggt
     3541 ttgagcagca aggatatcat tacttccctt aacgataccc cggtggaatc ggccagtgga
     3601 ttgcaggatc tgatcgccgc ccacaaggtt ggcgacaaag tatctttgaa tgtaatccgc
     3661 tccggcaggc aaatgacctt gaacgcaacg ctgcaggcca aacccaatgc ttaatctcaa
     3721 atgaaaaaaa tcaggggttt tcccctgatt ttttattgaa taagttcaac gatttcccgc
     3781 aaggctgtca gcgagctgcc tttttccatt tgtatgttca aggtgtaggg attcaccttt
     3841 ttcaagcccg gtactttgat attgttttcc aatggctggt taagcacaat ctcaatttta
     3901 tcacccttgc gatgcaggaa tttgatggcc ttgtcccggg ctttgatacg cagggccgcg
     3961 atttgcataa agttttccac tgcggtgggc agccggccaa agcgatcttc tatttcattg
     4021 cggatgtcct caatttcctc cgccgccccg gccaataaca actgccggta aatccgcatt
     4081 ttagtgccgg gatcagggat atacgtatcc ggaatgtaat aatccacgtc aatatccaac
     4141 tggggattga ccgtttccgt cgctacaatg cccctcaatt tggcggtttc ctgttccagc
     4201 agacggcaat agagatcaaa gcctaccgcg tagatatgtc cgtgctgttc tgctcctaaa
     4261 atattccctg ctcccctgat ctccagatcg cgcagcgcga ttttcatccc ggcacccagt
     4321 tcggtaaatt cccggatagc gttgagacgt ttttgtgcaa tttcggtgac agccttgtcg
     4381 ggacgatagg taagataagc atacgccacc cggtcggaac gtcctacccg gccacgcagt
     4441 tgataaagct gagccatacc catatggtct gccatatcga cgatgatggt attgacattg
     4501 ggcatatcca acccggattc aataatcgtg gtacataaaa acaagtcgaa cttgccttca
     4561 ataaattcca tgatagtccg ggccagttct tcttcccgca tacgcccatg ccccacagct
     4621 atctgaacct gaggcagcag tttatccaac tgcgctttga ccttttggat gtcctggata
     4681 cggttgtgta cataaaagat ctgcccgccg cgagcgaatt ccgtcatcac cgcttctttg
     4741 acgatctctt cgttatattc catcacataa gttgtaatgg gatagcgctg cggaggggga
     4801 gtctcaatca cgctcaaatc ccttagcccg gtcagtccca tatgcaagct gcgcgggatc
     4861 ggggtagccg aaagactcag cacatccacc tgggctttca gcgatttaat tttttccttt
     4921 tgggcaaccc caaaacggtg ttcttcatca atgaccagta atcccagatt cgcaaacata
     4981 atatctttgg ataacagacg gtgggtggca atcacaatgt ccaccccccc ctgattgatg
     5041 gcctgaacga tcttcttttg ctgggcagtc gtacggaaac ggcttaatac ttcgatgttg
     5101 gccggataat tctgaaaacg ctgctgaaac gtctggtaat gctgttcggc caaaaccgtt
     5161 gtcggcacca gaattgccac ctgcttgccg tccatgatgg ccttgaaggc tgcccgtaaa
     5221 gccacctcgg tcttgccata gccaacatca ccgcatacga tgcgatccat gggcttgctg
     5281 tcttccatat ctcttttggt gtcctcgatg gccttcagtt ggtcgggggt ctcctgataa
     5341 gggaaatcat cttcaaactg cctttgccag ggtgtatccg gggaaaaggc aaagccctgc
     5401 acactctccc gcgtggcata aagacgcagc aagtcctcgg ccatatcctg aatcgaatcg
     5461 gccactcgtt tacgagtcct ctcccattcc gttccaccca gtttgctcag acgcggactt
     5521 ttgtcttccg aactggaata acgaaacaac aattccagct tgtctaccgg cagatagagt
     5581 ttgtccgtgc cggcatattg cagcaaaagg tattcccggg taacaccatc gctttgtacc
     5641 tgggtgacgc cgcgataaat cccgatgcca tagttttcat ggacgacata gtcacccagc
     5701 ttcagatcct caatcagcag cctttgttca gcacttcttt tcaccttttt gccggagaag
     5761 ttttttttcg gccacaaatc tttctcgctg atcagagcca cgcccatatc cggacttata
     5821 aaaccccgcg aataaggcaa agaagtgaat ttgactccac ttacatgatg atccaacaac
     5881 tgtccggcca tttcttcctg taaattcttc tttttgatcg ccagcacgac ctcataatct
     5941 ttggcaagcc attcctgtaa gcgtggatac aggttttcca attggttgtg gaagggttcc
     6001 atgtctcgct gggcgatatg tttataaaag cccacttg
//
LOCUS       DAOTHZ010000103         5918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_15981_length_5918_cov_8.038547, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000103 DAOTHZ010000000
VERSION     DAOTHZ010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15981_length_5918_cov_8.038547"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..3457
                     /locus_tag="P5549_09465"
     CDS             104..3457
                     /locus_tag="P5549_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRW13340.1"
                     /translation="MPANFEFLQDQTEYALFASACLEAERVLMSSSAMAAVGCRRALE
                     LAVKWVYSADNTIEMPYKDNLQSLIHEPSFRFALDNQTWSKLPYIIKLGNLAVHTERG
                     ISRSDAILSLSSLFEFIQWIDYCYGENYEEHYFDEAKIPAEKIILDEARIKEKESIIE
                     QKDSEIAVLREKIASMSEQLTADKQQHKEERQFTPTDISEYRTRKKYIDVDLKLLGWV
                     FGDDVREEVELHGMPNPEGKGYADYVLYGKDGLPLAIIEAKRTSKDPRIGTQQAKLYA
                     DCLENMTGRRPMMFTSNGFETYLWDDVTSPQRKVSGIFSKSDLEKLMNRRSERKALND
                     IKIDDKITDRYYQKEAIRAVCENIEKGHRKSLLVMATGSGKTRTASSLTDVLSRGGYV
                     TNTLFLADRTALVKQAKDDFKNYLPDMSLCNLLSNKEDKTARIVFSTYPTMLNAIDSA
                     KNADGQRLFTPAHFDLIIIDESHRSIFKKYRAIFEYFDGLLLGLTATPKTEVDRNTYD
                     FFEMESGVPTYAYDYETAVETDHVLVPFHNIEVRTRFLEQGICYDELSEEDKARYEED
                     FTDEDGEMPDFIPPPAVNEFIFNQSTVDMVLEDLMTKGIRVAGGDRLGKTIIFAQNKK
                     HAQYIVERFNKLYPQYHGSFAKRVVCDDSYVQTLITDFKIAEKEPHIAVSVDMLDTGI
                     DVPEIVNLVFFKRVRSKIKFWQMIGRGTRLCKNLFGEGQDKTHFLIADYLGNFEFFRA
                     NQDGTQGNEAQSLSEKIFAKRIRLIHHLQQSAFIDEPYQTIRAGLIKAVLPQINELNT
                     ELISVKLQLQYIERYKKAEAFICLSDLDKHDLIVHLAPIVYMDDTDEFAKRFDNFMYG
                     LMIAHIEGMSYFKKAQRQLVNVCAGLAKRATIPQVKEKIEFINTIATDEFWQSSDILN
                     FERVRIELRSLIKFIVDEGGVNTIYTNLTDEVLEVKEGEVIDPAFTFEDYKLKVNRYI
                     EENRDQMAIYKLRNNIPLTALDYQSLEHILTGELGTAEDYKREFQDTPFGLLVRKIAK
                     LEYEAANMAFSEFINDQSLSQGQIVFVKKIIDYIVQNGYMENVSELMKPPFDHPQSFI
                     KLFDGSKQRKIVETIKKIKDNAVKIMGQG"
     gene            3680..4894
                     /locus_tag="P5549_09470"
     CDS             3680..4894
                     /locus_tag="P5549_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW13341.1"
                     /translation="MGMIEGFRIQNFGVLKDIKLGKLWNEKGNKLTPLVAVIGRNGVG
                     KSTLFDAFGFLSDCLKHGVEEACDLKARGGFSKIISAGSQEPIRFELYYRETTGERPI
                     TYELEIDIDSSQRPYVKSERLRQRRKGQSTGQPFSFLNLSNGKGEAWRGTSLGEEESE
                     KVSIELIDARHLGISTYGALKEHPRITAFREFLENWYLCYFEPDAARSLPLAGPQKYL
                     NLRGDNLGNVVQYMERDNPDQFKKILDKISSRIPGVNKISTKKTDDGRLLIQFNDKGF
                     TDPFYAQQMSDGTLKYFAYMLLLEEPTQRPFICIEEPENGLYHKMLESLAMEFKRYAI
                     ERKTKSQIFVTTHSPYFIDALNPEEVWILEKNETGFSTIRRASDDAIIKAMVEEGITL
                     GSLWFSDYLDKR"
     gene            4900..5553
                     /locus_tag="P5549_09475"
     CDS             4900..5553
                     /locus_tag="P5549_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HRW13342.1"
                     /translation="MHLEVLVEDQSGSILVEKILDKINGQQVNRFTFRIHKFKGSGKL
                     PHELFSRSDPQKRILLDRLPLLLRGYGKSLDNNSAVIVVVDNDTKNCRDFKQELQSIL
                     ASCNPAPNTAFCIAIEEMEAWLLGDRQAVKTAYPGMKVRVLDHYVQDSICGTWEILAD
                     AVCREKASGLKKLGYPEIGIRKSEWMEKIAPNINLERNSSESFQYFVRKVQQIIGVA"
     gene            complement(5573..5788)
                     /locus_tag="P5549_09480"
     CDS             complement(5573..5788)
                     /locus_tag="P5549_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13343.1"
                     /translation="MRFLDAIKKPFVIKRLKKEYKMLYGSTAAEAEQSLQRQLHFMKT
                     KKPGQSEEWYLRKIIYDLEKDRRRGRR"
BASE COUNT         1736 a         1197 c         1425 g         1560 t
ORIGIN      
        1 tttgacagtt taatgagtga gtattttgat gattagatgt attataaaat aaggtaatgg
       61 agtatattga atccagctca gggaataagg cttggtgata atattgcccg caaactttga
      121 attcctgcag gatcaaaccg aatatgcatt gtttgccagt gcctgtctcg aagcggaacg
      181 ggtgctgatg tcttcgtctg ccatggcggc ggtgggttgt cgccgggcgt tggagctggc
      241 agtgaaatgg gtatactcag cggacaatac cattgaaatg ccctataaag acaatctgca
      301 gtcgttgatt catgaacctt cctttcggtt tgccctggac aaccagacct ggagcaagct
      361 gccttacata atcaaactgg gcaacctggc ggtacatacg gaacgaggca tcagccgcag
      421 tgacgctatt ctgtcccttt cctctttgtt tgaattcatt caatggatcg actattgtta
      481 tggcgaaaat tatgaagaac actattttga cgaagccaaa attcccgctg aaaaaataat
      541 cctggatgaa gcccggatca aggaaaaaga aagtatcatc gagcagaagg attcggaaat
      601 tgccgtactg cgggaaaaga ttgcatcgat gagcgaacag cttaccgctg acaagcagca
      661 gcataaggag gagcgccaat ttacacctac ggatatttct gaatatcgta cccgtaaaaa
      721 atatatcgac gttgatttga aactcctggg ctgggtattc ggggatgatg tacgcgaaga
      781 agttgagctt cacggtatgc ccaatccaga aggcaaaggc tacgccgatt atgtgctgta
      841 cggcaaggac ggactgcccc tggccatcat cgaagccaaa cgaacctcca aagatccccg
      901 cataggaacc cagcaggcca aactctatgc cgattgcttg gagaatatga ccggccggcg
      961 ccccatgatg tttaccagca atggttttga gacctatttg tgggatgatg taacctcgcc
     1021 gcagcgcaag gtaagcggca ttttttcgaa atccgatctg gaaaaactga tgaatcgccg
     1081 cagcgaacgc aaagccttga atgatataaa gatagacgat aaaataaccg accgttacta
     1141 ccagaaggaa gccatccgcg cagtctgcga aaacattgaa aagggccacc gcaaatccct
     1201 gctggttatg gcgaccggct cgggcaaaac cagaacggcc tccagcctga ccgatgtttt
     1261 atcccggggc gggtatgtaa ccaacacttt gtttttagct gatcgaaccg ctttggttaa
     1321 acaggccaaa gatgatttta aaaattatct gccggatatg tccctgtgca atttactcag
     1381 caacaaagag gacaaaacgg ctcggatcgt gttttccacc tatccaacca tgctcaatgc
     1441 cattgattcg gccaagaacg cagacggcca gcggctgttt actccagctc attttgactt
     1501 gattatcata gatgaatccc accgcagtat tttcaaaaaa taccgggcca ttttcgagta
     1561 ttttgacggc ctgctgttag gtttgaccgc aactcccaag actgaggttg atcgcaatac
     1621 ttatgatttc tttgagatgg aaagcggtgt gccgacttac gcatatgact atgaaactgc
     1681 ggtagagaca gaccacgtac tggttccttt ccataatata gaggtcagaa ccagattcct
     1741 ggagcaaggc atttgttacg atgaactttc tgaggaagac aaggctcgct atgaagagga
     1801 ttttactgat gaagacggcg aaatgccgga ctttattccg ccaccggcag tgaatgaatt
     1861 tatctttaac cagtcaaccg tcgatatggt gctggaagat ctgatgacca aaggcatccg
     1921 agttgccggc ggggacagat tgggcaaaac cattatcttc gcccagaata aaaagcacgc
     1981 ccaatacatt gtcgagcggt tcaacaaatt gtatccacag tatcacggta gctttgccaa
     2041 gcgggtggtt tgcgatgaca gctatgtaca aaccctcatc accgatttta agattgccga
     2101 aaaggaaccg catatcgctg tgtctgtgga tatgctggac accggtatcg atgttcccga
     2161 gattgtcaat ctggttttct tcaaacgggt ccggtcgaaa ataaaattct ggcagatgat
     2221 tggccggggc accaggctgt gtaaaaatct gttcggggaa gggcaggaca aaacccattt
     2281 tctgatcgcc gattatctgg gcaacttcga atttttccgg gccaaccagg acggaacgca
     2341 aggtaatgaa gcccagagcc tttccgagaa gatctttgcc aagcgaattc gtttgatcca
     2401 tcatctgcag caatcggcct ttatcgatga accttatcag accatccgcg ccggtcttat
     2461 caaagctgtc ctgccgcaga taaacgagtt gaataccgag ctaatatcgg tgaaactaca
     2521 gctgcagtat atcgaaaggt ataaaaaggc ggaggctttc atttgccttt cggatttgga
     2581 caaacatgat ctgatcgttc atctggcacc tattgtttac atggatgaca cggacgaatt
     2641 tgccaaacgg tttgataatt ttatgtatgg tctgatgatt gcccacatcg agggaatgtc
     2701 ctattttaaa aaggctcaaa gacagcttgt caatgtgtgt gccggcctgg ccaaacgcgc
     2761 gaccatacca caggtcaaag aaaaaattga attcatcaat accatcgcga cggatgaatt
     2821 ctggcaaagc tctgatattt tgaacttcga gagggtgaga atagagctgc gcagtctgat
     2881 caagtttatc gttgacgaag gcggagtaaa cacgatctat accaacctga ctgatgaggt
     2941 tctcgaggtt aaagaaggag aggtcattga ccctgcattt acatttgaag actacaagct
     3001 caaagtcaac cgatacattg aggaaaaccg tgaccagatg gcaatttaca aactgcgcaa
     3061 caacatcccg ctgacggcac tggactatca gagtctggaa cacatattga ctggagaact
     3121 tggcacagcc gaggactata aacgggaatt ccaggatacc cctttcggcc ttctggtgcg
     3181 caagatcgca aaactggaat atgaagcagc caatatggca ttttcggaat tcatcaacga
     3241 ccagtccctc agtcaggggc agatcgtttt cgtaaaaaag attatcgatt acatcgtgca
     3301 aaacggctat atggaaaacg tatccgagct aatgaagccg cctttcgatc atccgcagag
     3361 ttttattaaa ctgtttgacg gatcaaaaca aaggaagatt gttgaaacga tcaagaagat
     3421 aaaggataac gcggttaaga tcatggggca ggggtaagac atttgtattt aagcaatttt
     3481 aaagagtttt gttcttgctt gtttaaagag gttttccata taatgtaact gtgttgtatg
     3541 ctattgacca taataagatt gaggtcttca aacgttttat tgagtacagc ttattcaaac
     3601 aattgttgtg aatttatcag aaatttagtt aacttattta ttctttatgg aatgatcaga
     3661 cagaaaagga gggcggaaca tgggcatgat tgagggtttc aggatacaaa attttggtgt
     3721 acttaaagat attaaactgg gaaaactctg gaacgaaaaa gggaataaat taacaccttt
     3781 agttgcagta atcggaagaa acggggtggg gaaaagcacc ttgtttgatg cttttggttt
     3841 tttgtcagat tgtctgaaac acggagtgga ggaagcttgt gatcttaagg cacgcggtgg
     3901 atttagtaaa atcatatctg ccggcagtca ggagccaatt cgttttgaat tatattatcg
     3961 ggaaacaacc ggggaaaggc caatcacata tgagctggaa atagatattg atagtagcca
     4021 gagaccatac gtcaaatcgg aaagactgcg gcaaagacga aaaggacagt caacgggtca
     4081 gccgttctct tttcttaatt tatccaacgg caagggggag gcatggaggg ggaccagtct
     4141 gggtgaagaa gagtccgaaa aggtttccat tgaattaatt gatgcgcggc acctggggat
     4201 ttcgacttat ggtgctctga aagaacatcc tcgcatcaca gcttttcgtg aattcctgga
     4261 gaactggtat ttatgttatt ttgaaccgga tgctgccaga agtcttcccc ttgccgggcc
     4321 gcagaagtat ctgaatttgc gcggagataa tctgggtaat gttgttcaat atatggagcg
     4381 tgacaatcca gatcaattta aaaaaattct tgataaaatc tcgtccagaa tccctggcgt
     4441 caataagata tcaaccaaga aaacggatga cggcagattg ttgattcagt ttaatgataa
     4501 gggttttact gatccgtttt atgcacagca aatgtcagac gggactttga aatactttgc
     4561 ctatatgctt cttttggagg aaccgaccca gagaccattt atctgcatcg aagagccgga
     4621 aaacgggctt taccacaaga tgctcgaatc gttggccatg gagtttaaac gttatgctat
     4681 agagcgtaaa accaaatctc agattttcgt tacgacccac agtccgtatt ttattgatgc
     4741 cttaaatccc gaagaggtat ggatactgga aaaaaacgaa accggttttt ccaccatcag
     4801 gcgggcaagc gatgatgcaa tcatcaaggc catggtcgaa gaagggatta cgctgggcag
     4861 cctgtggttt agcgattatt tggataagag gtgaagagta tgcacctgga agtattagtg
     4921 gaagatcaat cgggaagtat tttggtggag aagattcttg ataagattaa tgggcagcag
     4981 gtgaatcgct tcactttcag gattcataaa tttaaaggct caggaaaatt gcctcatgaa
     5041 ttattttccc gatcagaccc tcaaaaaaga atattgctgg accgactgcc tttattgctg
     5101 agaggatatg gcaagtcttt ggacaataat agcgcagtta ttgtggttgt cgataatgat
     5161 accaaaaact gccgggattt caaacaagaa cttcaaagca ttctggctag ttgcaatccg
     5221 gctcccaata cagctttttg tattgccatt gaagaaatgg aggcttggtt attgggtgac
     5281 aggcaggcgg ttaagacagc ttatcccggt atgaaagttc gcgtacttga tcactatgtt
     5341 caggatagta tttgtggtac gtgggagata ctggccgatg ctgtttgccg ggaaaaggca
     5401 agcggcttaa agaagcttgg gtatcctgaa ataggtataa gaaaaagcga gtggatggag
     5461 aaaattgcgc ctaatattaa tctggaacgt aatagctcgg aaagttttca gtattttgtc
     5521 aggaaagttc aacaaattat tggtgttgca tagaagcagt tccctgccgt tattacctcc
     5581 gcccccgtct gcggtctttt tcaagatcgt aaataatttt ccggagatac cattcttccg
     5641 attggccggg ttttttcgtt ttcatgaaat gcagttgtct ttgcagcgac tgttccgctt
     5701 cggcggcagt tgaaccatac agcattttat attccttctt taaccgtttg ataacgaacg
     5761 gtttttttat ggcatcaagg aaacgcattc gatgcagtcc ctccccttaa tatttttatt
     5821 tgctctttca ttttatataa aatgggaaaa ttatgcaaag ttccagataa ggtacaataa
     5881 atttcgcaaa ttgaaaaagc gtccagaaaa atagcgga
//
LOCUS       DAOTHZ010000104         5743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_16604_length_5743_cov_8.005450, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000104 DAOTHZ010000000
VERSION     DAOTHZ010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16604_length_5743_cov_8.005450"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="P5549_09485"
     CDS             complement(<1..402)
                     /locus_tag="P5549_09485"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641840.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRW13344.1"
                     /translation="MCFLKDNMVIVGGQPLYGNVRISGAKNAALVLIAASIMAEGETV
                     LDNVPRICDVEVMIDILNQMGVRTNWNDNGSLSVCPPAGDMNIKTPDELAKRLRASNL
                     FLGALLGRQGEAAVSMPGGCDIGSRPMDLHLK"
     gene            complement(407..1258)
                     /locus_tag="P5549_09490"
     CDS             complement(407..1258)
                     /locus_tag="P5549_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13345.1"
                     /translation="MWENRPNSTFVVLFIVLAVALGCYLYRSDIQSGQIWQKISRLAV
                     GQEIRFRTARFQEMKTDHFVIKFTQTDADYAAIIANSAERAYRQVSDRMNIDVRQKTT
                     IVVYPDSRSLARCFGWDKNEKALGVYWAGTIRILSPHEWLQQAYNEAEFQTEGPLVHE
                     YVHLIVDDMTGGNYNRWWTEGVAQYIEKQITGFQFADPQDVTAGQLYTFQELNKNFDE
                     LDQQMSYWQSRQAVEFMASQYGEEVIYTVMDYLAQGDTLYQAIEKATGTDFENFAYQC
                     YRYMEKQ"
     gene            complement(1335..2240)
                     /gene="trxB"
                     /locus_tag="P5549_09495"
     CDS             complement(1335..2240)
                     /gene="trxB"
                     /locus_tag="P5549_09495"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRW13346.1"
                     /translation="MYDVIILGGGPAGLTVGLYTGRAKLKTLILEASHIGGNAAWIDQ
                     LENYPGFPFGVSGGELMDNFHKQAERFGCETRMEEVIKLESLPEGKKVTTYEGEYWAQ
                     AVVIALGAKRRELEVPGEQEFKGSGVSYCATCDAAFFENYPVAVVGGGDTAVNEALFL
                     SGIASEVFLIHRREGFRANQTSVDKMLANDKIKLKTNKVVTRIEGEGLMQRVILQDVN
                     TREEEVLPVEGLFVSIGLVAAAEFLEGVVATQEGYVITDEEGRTSVEGIFAAGDIRVK
                     KTRQVATAVGDGSQVGMIVSSYLKE"
     gene            complement(2311..3651)
                     /locus_tag="P5549_09500"
     CDS             complement(2311..3651)
                     /locus_tag="P5549_09500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRW13347.1"
                     /translation="MQTVSRNQKLLAAGVAVLTVMLMCVWFLGFKTPAYAVYINGQQQ
                     FIVKDRGQVELAIKQISKQLGAEQNKKIVLSSKIEYKRIFAERKVLLAEQKINPALQK
                     GLKFQTNGAVIVVDGKVIASMQDKTAAASLLQKLKAANSWVGEDEKLISAAFAEKVTV
                     REAKVYAGEVLAPQEAWNLITTGTASPEKYKIKAGDSIWSIARANDMYVDDILKANNL
                     AEDDVLQLGREIVLVKSKPYINVIAKVEGKAIETIPFETKVITDNSVNNIKIKSNGAN
                     GKKEVAYIVEKINNRIEKKEVLSETILEKPKDKVMVKGTRVLQVASRSGSNTGTLAWP
                     VAGYISQYSRSGHVAIDIAGSTGSPIQAAESGTVTFAGWGEGGYGKCIVINHGNGLST
                     RYAHCASLAVSVGDSVGRGQTIATRGSTGRSTGPHLHFEVLSGGSQVNPLNYLR"
     gene            complement(3739..5121)
                     /gene="scfB"
                     /locus_tag="P5549_09505"
     CDS             complement(3739..5121)
                     /gene="scfB"
                     /locus_tag="P5549_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HRW13348.1"
                     /translation="MQRLTGYDFQANIHLFQWQQQNILLDVNSGAVHLLDDMSCAFIT
                     RLMEYNGDMERVVQELTGVYPADELHDIVEEISQAHQQGALFSEEELPLLDFSGFHVK
                     ALCLNVAHACNMKCRYCFASQGDFGMEAGLMPLETGKRALDYLMAQSGPIRNLEVDFF
                     GGEPLLVADMLRQLVAYGRELERQWNKRIHFTLTTNALLLTDDVIEFVLAEDISVILS
                     LDGRPETNDRHRILNNGAGTYELILPNIKKMVNANPISYYIRGTFTKKNLDFSEDLRH
                     MAELGFDAISLEPAVGEDNGFSIQEEDLEQVLAEYGRLTDLLNEYRLAGRKLDFFHYN
                     LNLQRGPCLAKRLSGCGAGNEYLVVIPNGDIYPCHQFVGEKEFIMGNIFDGHLDEGLK
                     KRFSQNHLQNKSECKSCWARYYCGGGCHANAYHANGDFSKPSQVSCRMHRKRIEGAIF
                     LETMEGLTNT"
     gene            complement(5220..5363)
                     /gene="scfA"
                     /locus_tag="P5549_09510"
     CDS             complement(5220..5363)
                     /gene="scfA"
                     /locus_tag="P5549_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HRW13349.1"
                     /translation="MKKHITTIINPNLKRDQEAINCRECGTSCQSACKTSCTVGNQNC
                     KNQ"
     gene            complement(5473..5547)
                     /locus_tag="P5549_09515"
     tRNA            complement(5473..5547)
                     /locus_tag="P5549_09515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5513..5515),aa:Gly,seq:ccc)
BASE COUNT         1366 a         1555 c         1228 g         1594 t
ORIGIN      
        1 tttaagatgc aggtccatcg gtcgggaacc gatatcacag ccgcccggca tggaaaccgc
       61 tgcctctccc tgtctgccca acagcgcacc cagaaataaa ttggaagccc gcagcctttt
      121 agccagttca tcgggtgttt taatgttcat atcacctgcc ggcggacaaa ccgacagact
      181 gccattatcg ttccagttgg tcctgacccc catctggttt agaatatcta tcatcacttc
      241 gacatcacaa attcggggta cgttgtcaag gaccgtttct ccttccgcca taatactggc
      301 cgcaattaaa accagcgcgg catttttggc tccgctgatc cgcacattgc cgtataatgg
      361 ttgtcccccc acaataacca tattgtcctt caagaagcac acctctctat tgcttttcca
      421 tatatcgata acattgatat gcaaaatttt caaaatcagt accggtcgct ttttcaatcg
      481 cctggtaaag cgtatcacct tgtgccaaat aatccattac cgtataaatc acttcttccc
      541 catattgaga ggccatgaat tcaaccgcct gacgggactg ccaataagac atctgctgat
      601 ccagttcatc aaaattcttg ttcagttcct gaaaagtata taactgtccc gcagtaacat
      661 cctggggatc tgcgaactga aagccggtga tttgtttttc tatatactgg gcaacccctt
      721 ccgtccacca gcgattataa ttccccccgg tcatatcgtc aacgattaaa tgtacatatt
      781 catgcaccaa cggcccttcc gtctggaatt cagcttcatt gtacgcttgc tgcagccatt
      841 catgcggtga caggatacga atcgtacccg cccagtagac tcccagggct ttttcgtttt
      901 tgtcccagcc aaaacaccgg gccagacttc ggctgtccgg atagaccaca atggtcgttt
      961 tctgtcgaac gtcgatattc atcctatcac tcacctggcg atatgcccgc tctgcactat
     1021 tggcaataat agcagcataa tctgcgtcgg tttgtgtaaa ttttatgaca aaatgatcgg
     1081 tcttcatttc ctgaaaccgg gctgttcgga atctgatttc ctgtcccact gccaggcggg
     1141 aaattttctg ccagatctgt ccggactgaa tgtcgcttct gtacagatag caacccaagg
     1201 caaccgccag cacaatgaaa agcacgacaa acgtactgtt tggtctgttc tcccacatac
     1261 cagcccctcc cctctaccca tagattatac tatgggcagc gcttgcgagg ggcaagcaaa
     1321 tactggtata tcagctattc cttcagatag gaactgacga tcattcccac ctgggaacca
     1381 tcgcccaccg ccgttgccac ctgacgggtt tttttcacac gaatgtcacc ggcggcaaag
     1441 ataccttcta cagaagttcg tccttcttca tcagtgatga catagccttc ctgggtggca
     1501 acgacgcctt ccagaaattc agccgccgct accagaccga tgctgacaaa aagaccttcc
     1561 accggcagga cctcttcttc ccgggtgttg acatcctgca gaatcactct ttgcatcagt
     1621 ccttctccct cgatacgggt caccacttta ttggtcttga gttttatctt gtcattggcc
     1681 agcatcttgt caaccgaggt ctgattggca cggaaaccct cccggcgatg aatcagaaat
     1741 acctcggagg cgattccgga aagaaacagc gcttcattga cagctgtgtc gccgccaccc
     1801 accaccgcca ccgggtagtt ttcaaaaaat gcggcgtcac aagtggcaca gtaggaaacg
     1861 ccgctgccct tgaactcctg ttcgccgggt acttccagtt ctctgcgttt ggctcccagg
     1921 gcgatgacga ccgcttgggc ccagtactcg ccttcataag tcgtcacttt tttgccttcc
     1981 ggcagagatt ccagcttgat aacctcttcc atacgagtct cacaaccaaa gcgctccgct
     2041 tgtttgtgaa agttgtccat cagttctccg ccggaaaccc caaaaggaaa tccgggataa
     2101 ttttccaatt ggtcgatcca ggccgcattg cctccgatat gactggcctc cagaatcaac
     2161 gtctttaatt tggcccgccc ggtataaaga ccaacggtta acccggccgg cccgcctcct
     2221 aaaataataa catcgtacac tttaatcgct ccttttcgtg tgttggtaaa aaaacaaccc
     2281 tgacccaagg ccagggttgc aggaaaataa tcagcgtaga taattcagcg gatttacctg
     2341 actgccgccc gataaaacct caaaatgcag atgcggtccg gtgcttctgc cggttgagcc
     2401 acgcgtcgca atcgtttgac cgcggcctac gctgtcaccc accgaaactg cgaggcttgc
     2461 acaatgggca tagcgggtgg aaaggccatt gccgtgattg ataactatgc atttgccata
     2521 accaccttca ccccatccgg caaaggtcac cgttcccgac tcggctgctt gaatgggtga
     2581 gcctgtactg cctgcaatat cgatggccac atgtccgctt ctggaatact gggatatata
     2641 tcccgctacc ggccaggcca gtgtaccggt attgctgccg gaacgggagg ccacctgcaa
     2701 taccctggtc cctttgacca taaccttgtc tttgggtttc tcgagaatcg tttccgacaa
     2761 gacttccttt ttctcgatac ggttgtttat tttttcaacg atgtaggcca cttctttttt
     2821 gccgttggcc ccattgcttt taatttttat gttgttgacg ctgttgtcgg ttatcacctt
     2881 ggtttcaaat ggaatggttt cgatggcctt gccttcaact ttggcaatga catttatgta
     2941 tggcttgctt ttcaccaaaa cgatttcccg gcccagttgc agcacgtcat cttccgccag
     3001 gttgttggcc ttcaggatat catccacata catgtcattg gcacgggcga tgctccaaat
     3061 gctgtcccct gccttgatct tgtatttttc agggctggcg gtacccgtgg taattaaatt
     3121 ccaggcttcc tggggtgcca atacctcccc tgcataaacc ttggcttccc gaaccgtgac
     3181 cttttctgca aaagcggcag agatgagttt ttcatcttcc cctacccagc tgttcgctgc
     3241 ttttagtttt tgcagcaggg acgcagcggc agtcttgtcc tgcatactgg caatgacttt
     3301 gccatcgacg acaattactg caccgttggt ctgaaacttc agtccttttt gcaatgccgg
     3361 gttaattttt tgttcggcca aaagaacttt tctctcagca aagatacgct tgtattcaat
     3421 cttgctgctg agaacgattt ttttgttttg ctccgccccc agctgtttgc tgatttgttt
     3481 tatcgccagc tcaacctggc cacgatcttt gacgatgaat tgctgttgtc cgttaatata
     3541 gacggcatac gccggagtct taaatcctaa aaaccaaaca cacatcaaca tcactgtcaa
     3601 cacggcaacg cctgctgcca gcagcttttg atttcttgaa acagtttgca tattcaatct
     3661 ccattgttat attatttctc agattcattc cctatttcta caacaaatcc aaatcttcct
     3721 tctattttct taattttatt aagtgtttgt caacccttcc atggtttcca ggaaaattgc
     3781 accttcaatt cttttccgat gcatgcggca gctgacctgg ctgggcttgg agaaatcacc
     3841 attggcatgg taggcattgg catggcagcc accgccgcaa tagtacctgg cccaacagct
     3901 tttgcattcg ctcttgtttt gtaaatggtt ttgggagaat cgttttttca acccctcatc
     3961 caaatgaccg tcaaaaatat tgcccatgat gaattctttt tctccgacaa attgatgaca
     4021 cggataaata tcaccgttgg gaatcacaac caggtattca ttgcctgccc cgcatcctga
     4081 cagacgcttg gccagacagg gccctctttg taaattaaga ttgtaatgga aaaaatcgag
     4141 cttgcgtcct gcaagacgat attcattgag aagatcggtc aagcgtccat attccgccag
     4201 cacttgttcc aggtcttctt cctgaatgct aaagccgttg tcttctccca ccgccggttc
     4261 caaggatatc gcatcgaaac cgagttcggc catgtggcgc agatcttcgc tgaaatcgag
     4321 attctttttg gtgaaagtcc cacgaatgta gtaactgatc ggattggcgt tgaccatttt
     4381 tttaatgttg ggcaggataa gctcatatgt cccggcacca ttgttgagaa tccggtgtcg
     4441 atcattggtt tccggtcgtc cgtccaggct gagaatcaca ctgatatctt ctgccagcac
     4501 aaattcaatg acatcatcgg tgagcaagag cgcattggtg gtcagggtga agtgtatgcg
     4561 tttgttccat tggcgttcca gttcgcggcc ataagccacc agttgccgaa gcatatccgc
     4621 taccagcaat ggttcgccgc cgaaaaaatc aacttccaga ttgcgaatgg gaccgctctg
     4681 tgccatcagg taatccaaag cacgcttgcc ggtctccaga ggcatgagtc cagcttccat
     4741 gccaaaatcg ccctgactgg caaaacagta gcggcatttc atgttacagg catgggccac
     4801 atttaaacaa agcgccttga catgaaaacc ggagaaatcc aggagcggca attcttcttc
     4861 agaaaacaac gctccttgct gatgcgcctg gctgatttct tccactatat cgtgcagctc
     4921 gtcggctgga taaacgcctg tcagttcctg cacaaccctt tccatatcac cgttgtactc
     4981 catcaggcgg gttataaaag cgcagctcat gtcgtccagc aggtggacgg cgccactgtt
     5041 gacatccagc aaaatatttt gttgctgcca ttgaaacaaa tggatattgg cctgaaaatc
     5101 atagccggtt aatcgctgca agaaattctc ctcgtttcac cccatgataa aatcctgtaa
     5161 ataggctgct aaacaaaaac aggggctttt cgcccctatt aaagaattct atgtaattcc
     5221 tactggtttt tacagttttg gttgcctacc gtacaggagg tcttacatgc ggattgacag
     5281 gaagtaccgc actcccggca gttgatggct tcctggtctc ttttcagatt gggattgatg
     5341 atcgtggtaa tgtgtttttt caaactccaa acccccttcc ccttatcaac ttggaatccc
     5401 gaatacggga tatgtaaaac ttgccatgtc catcgcaggg gggactgccc taccgcggta
     5461 cgaatatgtt tatggagcgg gaaaaggggt tcgaaccctc gacacccggc ttgggaagcc
     5521 ggtgctctgc cactgagcta ctcccgcaat atagagtatt atacactatc gatttttgat
     5581 ttgacagaat tttgacagaa gtttttacga ttatttttca caactaactg tcgggcagtt
     5641 cttgctttct atctcaattt cttagaaacc cgacggtaaa tcgtatatag atgccgggta
     5701 tcagttttgc cctaggggtg agctaaaata ccaaaccctc atc
//
LOCUS       DAOTHZ010000105         5670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_16875_length_5670_cov_7.089403, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000105 DAOTHZ010000000
VERSION     DAOTHZ010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16875_length_5670_cov_7.089403"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..778)
                     /locus_tag="P5549_09520"
     CDS             complement(29..778)
                     /locus_tag="P5549_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13350.1"
                     /translation="MLKKTRLQKSIVLLLALLLLLAPAFSAVGAGTGSVEKMQDNELV
                     LSSLDDSGNISGIQVLSHVRIFGSGDVSVQDPTQFKLDSVRNLYGSEKIAQQDGKINY
                     SLNIDQSKGFSDIYYLSALNKEDVAKVKIPVSVKVTYYLDGQQIEPSKLAGKSGHLKI
                     RCDIENLTGAKKVLEYTNKAGETVKKESIVYTPYVVAMSGWTFDNKRFANVQAPGVAQ
                     ESPQGVVLDVQGKTQVSWTVPVIPPSYPAKQ"
     gene            complement(796..1290)
                     /locus_tag="P5549_09525"
     CDS             complement(796..1290)
                     /locus_tag="P5549_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13351.1"
                     /translation="MKRAIGLGLLVAVFFAAFIMAKDYIAVSSFYMAAAAMAMFAVLG
                     PFGDYFKTGISMIIGVVVGLVGIIILIAKMPLPPDNLVYLAAVGGVSLFILILLSVTG
                     LRIDGMFLGWAGYFASVYGTYTANAASLATGAVPAAVGVCVSLLVGLVMANLIIKIAM
                     ALNN"
     gene            complement(1492..1920)
                     /locus_tag="P5549_09530"
     CDS             complement(1492..1920)
                     /locus_tag="P5549_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRW13352.1"
                     /translation="MAKQLSVFLENKAGRLARVTSVLGEAGINIRALSIADTSDFGIL
                     RIIVNDPSQAYTILKDAGFTVSETEVIAVRVQDTPGGLAAVLEQMSNANLNIEYMYAF
                     LGTSEEDALVIFKIEDTKKARAAFKEKGIQVIEEQELYRL"
     gene            complement(1933..3234)
                     /locus_tag="P5549_09535"
     CDS             complement(1933..3234)
                     /locus_tag="P5549_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRW13353.1"
                     /translation="MLYWDQEKECMPRENLRELQLRRLKETVYRVYAFVPAYRAKMDE
                     AGIKPDDINSLDDIKYLPFTTKQDLRDNYPFGLFAIPMSEVIRIHSSSGTTGKPTVVG
                     YSRKDLDTWAELMARALYSAGARKRSVIQNAYGYGLFTGGLGMHYGAEKIGASVIPTS
                     GGNSKRQIMLMQDFGSTLLTCTPSYALFLAEIMQEMGIDPQDLKLEAGVFGAEPWSEN
                     MRRELESKLHITAFDIYGLSEIIGPGVAIECERRKGLHIAEDHFIPEIIDPVTREILP
                     EGSQGELVLTTITKEALPIIRYRTRDLTTLNHEICPCGRTHVRMHKVLGRSDDMVIIR
                     GVNVFPSMVESVLLNIPGVEPFYVLIVDRIGNLDILEVQVEVSERIFSDEVRKLEELG
                     KHITKELESALGVSVKVRLVEPKTLERSEGKAKRVIDRRIF"
     gene            complement(3571..3748)
                     /gene="ssrS"
                     /locus_tag="P5549_09540"
     ncRNA           complement(3571..3748)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5549_09540"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3870..5630)
                     /gene="aspS"
                     /locus_tag="P5549_09545"
     CDS             complement(3870..5630)
                     /gene="aspS"
                     /locus_tag="P5549_09545"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRW13354.1"
                     /translation="MKRTHNATGLTLEHAGEEVVLNGWVDNRRDHGGLIFVDLRDRSG
                     IIQIVFNPELNEQAFHLAESVRSEYVVSCRGKVVARSEANINPNLETGRIEVHIESME
                     VLNIAKTPPFYIENGIDVDEMLRMRYRYLDLRRPEMKDNLMLRHRVINTIRQFLDEEG
                     FIEIETPILGRSTPEGARDYLVPSRIHPGEFYALPQSPQQYKQMLMVAGMEKYFQIAR
                     CFRDEDLRADRQPEFTQLDMEISFIDEEDVFTLIESLMQKVFKVSNHIDLAIPFPRMT
                     YAEAMLKYGSDQPELRFGWEIQDITGLVRDVEFKVFSSAIAAGGVVRALNAKGCASFT
                     RKEIDDLGKTAMAFGAKGLAWIIVTENEIRSPITKFFTEEQMQAVLQELQAEPGDLLL
                     FGADQAAIVARVLGTLRLELGRKLKLYDENDLSFVWVVDFPLFEFDQEEQRYAAVHHM
                     FTSPREEDLELLERDPLAVRARAYDLILNGIELGGGSIRIHRRDWQEKVFSLLGMTPE
                     EANDKFGFMLEAFEYGTPPHGGVAFGIDRMIMLMAHRDSIRDVIAFPKTQSAACLMTD
                     APSVVSPRQLRELSLKLRDK"
BASE COUNT         1466 a         1499 c         1209 g         1496 t
ORIGIN      
        1 tatttttggc atctgcttcc agaatcgtct attgtttggc cggataacta ggcggtataa
       61 ccgggactgt ccaactgacc tgggtcttgc cctgcacatc cagtaccacg ccctgcggtg
      121 actcctgcgc tacccccggc gcctgaacat tggcaaacct cttgttgtca aaagtccagc
      181 cggacatggc aaccacataa ggtgtgtaaa caatcgattc tttcttgaca gtctcgcctg
      241 ctttgtttgt atactccagg actttcttgg cgccagtaag gttttcaata tcacatctta
      301 tcttcaggtg tccgctttta cctgccagtt tggaaggttc gatctgctga ccatcgagat
      361 aataggttac ttttacgctt accggtattt taactttcgc aacatcttcc ttgtttaagg
      421 ctgaaagata gtaaatatcc gagaagcctt tgctttgatc gatattcaag gaatagttga
      481 tcttgccatc ctgttgggca attttctcag atccataaag attgcgtacg gaatccagct
      541 tgaattgggt gggatcctgt acggacacat caccgctgcc aaaaatgcga acatgggaca
      601 atacctgaat gccactgatg ttgccggaat catccaggct ggacagtacc agttcattgt
      661 cctgcatttt ttccacgctg cctgtaccgg cacccacggc actaaacgcc ggtgccagca
      721 ataagagcag ggccagcaat aaaacgatcg acttttgtaa tcttgtcttt tttagcaaac
      781 cttttccccc ctttcctaat tattcagagc catagcgatt ttaatgatca ggttggccat
      841 aaccagtcca accaacagcg atacacatac gccgactgct gccggtacgg caccagttgc
      901 cagggatgct gcattggcag tataggttcc atataccgat gcaaaatatc cggcccaacc
      961 caggaacatt ccatcaatcc tgagcccggt tacagacaat aaaatcagaa tgaacagaga
     1021 tacgccgccg actgctgcca aataaaccag gttatccgga ggaagcggca ttttggcaat
     1081 aagaataata attcccacga gacctacgac aaccccaata atcatagata tacctgtttt
     1141 gaaataatcc ccaaagggac ccagcacagc aaacattgcc attgccgctg cagccatgta
     1201 aaaggaactt acggcgatat agtctttagc catgataaat gctgcaaaga atactgccac
     1261 cagtaatccg agaccaattg ctcttttcat tagttcacct ccttttttgt gtttttccgt
     1321 tccttgctgc gtaaagagtg caaatgttgc tgcaattcag gggaaaccaa ggttttatat
     1381 aaccattaag gagactacat gttttctcta gctgctaaag tatattcccg gcagcatgtg
     1441 ttttatgagc aacaagcggc tgtcatccgc agagaggatg aaagccgctg cctatagccg
     1501 atacaattcc tgttcttcaa tgacctggat acctttttcc ttgaaagcgg cccgggcctt
     1561 tttggtgtct tcgatcttaa agattaccag ggcatcctct tcagaggtac ccaggaaggc
     1621 atacatgtat tcaatattca agtttgcgtt tgacatctgt tcaagaacgg cggccagtcc
     1681 gcctggggta tcctgtaccc ggacggcgat gacttcggtt tcgctgacag taaagccggc
     1741 atcttttaaa atggtatagg cttgagaagg atcattgacg atgatgcgca ggataccaaa
     1801 atcagaagta tccgcaattg aaagggcgcg aatattgatg ccggcttccc ctaacacgct
     1861 ggtcacccgg gccagtcttc cggccttgtt ttccagaaaa acggaaagtt gttttgccat
     1921 acccttgcct ccctaaaaga ttcttcggtc gatgactctt ttggccttgc cttcacttct
     1981 ttccaatgtc ttgggttcaa ccaggcgaac cttgaccgaa acgcccagtg cactttccag
     2041 ttccttggtg atatgtttgc ccagttcttc cagttttctg acttcatcac taaagattct
     2101 ctccgacact tcaacctgta cttccagtat atccaggttg ccgatgcgat caacaatcag
     2161 cacataaaac ggctcgaccc cgggtatatt tagcagaaca ctttccacca tggacgggaa
     2221 tacattgaca ccccggataa tcaccatatc atcggaacga cccaggactt tgtgcatcct
     2281 gacgtgggtt cgtccacatg ggcaaatttc atggttcagg gtcgttaaat ctctggtcct
     2341 gtacctgata atgggcagcg cttctttggt aatggtcgtt aaaaccaatt ccccctggga
     2401 accctcaggt aaaatctccc gggttacggg atcaataatt tcaggaataa aatgatcttc
     2461 agcaatatgc agtccctttc tccgctcaca ttcgatggct actccaggac cgataatctc
     2521 gctgaggcca tagatatcaa aagcagtaat atgcagcttg ctttccaact cgcgacgcat
     2581 attctctgac cagggctcgg caccgaatac gcctgcctcc agtttcaaat cctgcgggtc
     2641 gatccccatc tcctgcatga tttctgccaa aaacaaagcg taggatggag tacaagtcag
     2701 caaagtggac ccaaaatcct gcatcaacat aatctgccgt ttgctgttgc cgcctgaagt
     2761 tgggatcacg gaagcaccaa tcttttccgc tccgtaatgc atccccaaac caccggtaaa
     2821 caacccatat ccataggcat tttgaatcac tgatctttta cgggcaccgg cactatacaa
     2881 ggccctggcc atcaattcag cccaagtatc cagatctttt ctggaataac caaccaccgt
     2941 tggttttccc gtggtgccgg aggaagaatg aatcctgatg acctctgaca tgggaatcgc
     3001 aaataaccca aacggataat tatcacgtaa atcctgtttg gtggtaaagg gcaaatattt
     3061 tatgtcatcc agggaattga tatcgtccgg tttaatgccg gcttcatcca ttttggcacg
     3121 atacgccggc acaaatgcat aaacccggta aaccgtttct ttcagccggc gcagctgcaa
     3181 ttcccggagg ttttcgcgag gcatgcattc cttttcctga tcccagtaca acattatttc
     3241 tactcctttc attgtttttc ctgcaacact tcgcaaatat tatcaattac cgtaataatt
     3301 aaatccactt tgaatttgca gccattttga ttctcgggtt tgtttgttgt tgagacctat
     3361 ccaggaggat atttgtcaat tgcttgatga ttaactgtaa atccagtgaa gcgtggtttt
     3421 catcattcta acatttcgtt ttgttataaa ccactatttg tatgtaattt tattgcagcg
     3481 gtcggttctc caaaaaaact gcactcatca gcaatgcaaa agagaaaaaa aatccggttt
     3541 gacaaccgga tgattatacc ctattcaaat gaaaacccca ccgagccgtg gtgccgcgag
     3601 ttttaacctg catagcaggt gggtgccctc ccgcttactc tttgtgtccc atgcaaaacg
     3661 ggcatgcaat tcacaagcga agcacaggct cccatagttt ctgtgttggc aaaaactata
     3721 cggcgattca cgaacgcagc agggctgata ctcaatgttc ctgcaaaggc tttacatcgc
     3781 aagtatacca cataacgcaa gccgcttcaa tgtgtccggc atccagtttc tccctgatat
     3841 aatgtactac atcatatgat gcaacccttc tatttatctc ttaatttcag agataattcc
     3901 cgcagttgcc gcgggcttac aaccgaagga gcatcggtca tcagacaggc cgcactctgt
     3961 gttttgggga aggcaataac gtcacggatg ctgtcccgat gagccatcag catgatcatg
     4021 cggtcaatgc caaatgccac cccgccatgc ggaggcgtgc catattcgaa agcttccagc
     4081 ataaagccga acttgtcatt ggcttcttcc ggagtcattc ccagcagact gaaaactttt
     4141 tcctgccagt cacgacgatg gatcctgatg ctgccgccgc ccaactcaat cccgttgaga
     4201 atcaggtcat aagcacgcgc cctgacagcc agcggatcac gctccagtaa ctcaaggtct
     4261 tcctcccggg gagatgtgaa catatgatga acagccgcat agcgctgctc ttcctgatcg
     4321 aactcgaaca aggggaagtc caccacccag acaaaggaaa gatcgttttc gtcatatagt
     4381 ttcagctttc tgcccagttc cagacgcaac gtccccaaaa cccgggccac aatcgccgcc
     4441 tggtcagcac caaacaacag aaggtcaccg ggctctgcct gcaattcctg caatacagct
     4501 tgcatttgct cctcggtaaa gaatttggtg atgggggaac gtatttcgtt ttcggtgaca
     4561 atgatccaag ccaggccttt ggcaccaaaa gccatcgccg ttttacccaa atcatcaatc
     4621 tctttgcggg taaaggaggc acagcctttg gcattgagag cacggacaac gcctccggca
     4681 gcaatggcac tcgagaacac tttaaactcc acgtccctca ccagcccggt gatgtcctga
     4741 atctcccagc caaaacgcaa ttcgggctgg tcactgccat atttaagcat ggcctcggca
     4801 taggtcatcc gcggaaacgg gatagccagg tcgatatgat tggaaacttt gaaaactttt
     4861 tgcataagcg attcaatcaa agtaaaaaca tcttcttcat cgataaagga aatttccatg
     4921 tccaactggg taaattccgg ctgccggtcg gctcgcagat cctcgtcacg gaaacagcgg
     4981 gcgatttgga aatacttctc catgccggct accatcagca tttgtttgta ttgttggggc
     5041 gactggggca gcgcataaaa ttcacccgga tgaatcctgc tcggaaccag ataatcacgt
     5101 gccccctccg gggtgctgcg gcccagtatc ggggtttcga tttcaataaa accttcttca
     5161 tccaggaact gccgtattgt attgatgacc cggtgacgca gcatcagatt atccttcatt
     5221 tccggacggc gcaggtcaag ataacgataa cgcatgcgca gcatttcatc tacatcaatc
     5281 ccgttttcaa tgtaaaaggg cggggtcttg gcgatattta atacttccat ggattcaata
     5341 tgtacctcaa tacgtcctgt ctccaaattg ggattgatgt tggcttcgga acgtgccact
     5401 acttttcccc ggcaggaaac cacatactca cttctgaccg actcggccag atggaatgct
     5461 tgctcattca gttccggatt gaaaacgatc tggataatcc cggaccgatc ccgcagatcg
     5521 acaaaaatga gcccgccatg atcgcggcgg ttgtctaccc acccgttcag gacgacctcc
     5581 tcgcctgcat gttccaaagt caatccggtt gcattatgcg tccttttcat tctggcaata
     5641 tcctcccaat ctattcaaga attgctttaa
//
LOCUS       DAOTHZ010000106         5555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_17359_length_5555_cov_7.901455, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000106 DAOTHZ010000000
VERSION     DAOTHZ010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17359_length_5555_cov_7.901455"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="P5549_09550"
     CDS             complement(<1..496)
                     /locus_tag="P5549_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW13355.1"
                     /translation="MLEFIQQMVNGLSLGAIYALIALGYTMVYGIIKLINFAHGDIMM
                     VGAFVGYFSLTLLGTNIIVAMVFSMLVCAILGVLIEKIAYRPLRNSTRIAALITAIGV
                     SLFLEYATIFILGPAQRVFPEAAFKVKAYNIAGLSVLNKDVFVIISAVVLMLILQYII
                     KWTKT"
     gene            complement(564..1748)
                     /locus_tag="P5549_09555"
     CDS             complement(564..1748)
                     /locus_tag="P5549_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW13356.1"
                     /translation="MKKSSKRVAMLVLMVFMLAIVAGCGGGGTGDKTTEQAEKINVGV
                     NVELSGGVASYGTNARDGAILAIEEINQAGGVLGKQLNPIERDCKSVADEAMSVSAAL
                     VGEKIVAQIGPLTSGNVAGSTPVMMDNKIPLIAPAATAANVTVDDKTGATRDYIFRVC
                     FIDPFQGTLMAQFAADNLKAKKAVIYGDTSSDYGKGLAEYFKKTFVEKGGEVIAEEGF
                     VKDDRDFRAALTKIKGLNPDFIYIPGYYQEVAPLIKQARELGITAPMGGGDGWDSPDM
                     VSVAGAAALNNTFFTNHYSSQDKDPKIVKFVEAFKAKYNKEPDAFAALGYDAVQLMVQ
                     GLKDAGEADPVKLAAAIAQIKDFEGITGKMSIDEQHNPVKAGVVIEFKDGAQIMNTRI
                     QP"
     gene            complement(2127..4031)
                     /gene="selB"
                     /locus_tag="P5549_09560"
     CDS             complement(2127..4031)
                     /gene="selB"
                     /locus_tag="P5549_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRW13357.1"
                     /translation="MKRLIIGTAGHIDHGKTTLVKALTGFETDRLKEEKQRGISIELG
                     FAPFSLPSGQKAAIVDVPGHERFIRHMLAGAFGIDMVLFTIAADEGVMPQTREHMDII
                     ELLGVDKGIIVITKKDMVDEEWLMMVEEEVSEYTAQGILKGCPIVAVSAVTGEGIKEL
                     LTTIEEVAADVVEKPVFGQARLPIDRVFSIAGFGTVVTGTLWSGQINVGDSLELMPVQ
                     RMVKVRTLQVHNEKVPTAYAGQRVAVNLQGVDLADIKRGYLLASSGYLEPSYRVNARL
                     RLLSSSPRTLQNWNRIRFHLGTDETLGRIVLLDRDELLPGDEAYAQIVMEKQVVAYKG
                     DPFVIRYYSPVNTIGGGTIIDPNATRQKRFKEEVLNDLMVKEEGSLYDLVLHELETSG
                     TEPLTIQEIARRTGNTEANVETEVQQLLDDEKIIDTSGKGAYYFSAQAVQAMGLSISK
                     TIAEHLQKYPLRQGYPKEDMRSRGFNQLNPKVFSGLLKYYEDHGVLQSQNNLITLPNY
                     VAHPGDKEQKIIAEIKQKFQENEFNPPGWDEIDVLKGLEEAQRNEIISYLIDSGYLLK
                     ISDNIYFSTAAMEKGKALLAEYFTKEKELSLATARDIFNTSRKYALPLVEYYDRIRFT
                     RRVGDMRIKA"
     gene            complement(4139..5536)
                     /gene="selA"
                     /locus_tag="P5549_09565"
     CDS             complement(4139..5536)
                     /gene="selA"
                     /locus_tag="P5549_09565"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRW13358.1"
                     /translation="MTNLRNIPSIDELLAQPETRHLLAVYQREFIVDILRRAVSDVRT
                     ELRTSRQTIAKAEISQLVLQKADVFLKNTVRGSLNRVINATGVVLHTNLGRAPLADRA
                     FQYMQATAQRYNDLEFDLAEGHRGSRYTHVEELLVRLTGAQAALVVNNNAAAVLLGLS
                     TMAQGREVIVSRGQLIEIGGAFRIPEVMKASGAKLVEVGTTNRTYLRDYEAAITEDTA
                     LLFSAHTSNYKIMGFTAEAGLDELAALGQARGLPVMHDLGSGILVDLGEWGLKDEPTV
                     PDSINSGADIITFSGDKLLGGPQAGIIVGKQKHIEAMKKNQLTRALRVDKLTIAALEG
                     TLLEYLSGDPRQHIPVIRMLTLPLEQLRENAEELREKLEQTLDGNPQVSKLEVIALED
                     MVGGGAYPTYKLPGYGVQLALRGKSLEAAARALRMLEPALLTRRQEDALLLSVRTLLP
                     GDQEEVAGLLAQVLQ"
BASE COUNT         1223 a         1499 c         1343 g         1490 t
ORIGIN      
        1 cggttttggt ccatttgatg atatattgaa gaatcagcat cagcacaacg gctgaaataa
       61 tcacgaatac gtccttgttt aaaacggaca gacctgcaat attgtaggct tttactttaa
      121 aggcggcttc gggaaatact ctctgggccg gacccagaat aaatatcgtc gcatattcga
      181 gaaacaagga aacgccgatg gcagtaatca gtgccgcgat acgggtcgag ttgcgcaagg
      241 gacggtaggc gatcttttcg atcaatacgc ccaggatggc gcagaccagc atggagaaca
      301 ccatggctac aatgatgttg gtccccaaca gggtcaggga aaaataaccc acaaaagcgc
      361 ccaccatcat tatatctccg tgggcgaagt tgattagttt aatgatgccg tagaccatcg
      421 tatagccgag agcgatcaag gcataaatcg cacccagcga caagccgttg accatctgtt
      481 gtataaattc cagcaaaatt gacacttcct cctgctgtca aagaggaaga gaggcttgtt
      541 cctctcttcc tctacagcgc ttgttacggt tgaatcctcg tattcattat ctgggcacca
      601 tctttgaatt caattacgac gccggctttt accggattgt gttgttcatc gatactcatt
      661 ttgccggtga tcccttcaaa gtctttgatt tgggcaatgg cggcggccaa tttgaccgga
      721 tctgcctcgc cggcatcttt cagaccctgc accatcagct gtacagcatc gtagccaagg
      781 gccgcaaaag catcaggctc tttgttgtat ttggctttga aagcttcaac aaatttaaca
      841 attttggggt ctttgtcctg ggaagaataa tggttggtga aaaatgtatt gttcagcgcc
      901 gctgctccgg ccacactgac catatcagga gaatcccagc catcgccgcc gcccatggga
      961 gcggtgatac ccagttcacg cgcctgtttg atcaggggag cgacttcctg ataatatccg
     1021 gggatataaa tgaaatcggg attgagtcct ttgatcttgg tgagcgctgc tctgaaatcg
     1081 cgatcgtctt ttacgaaacc ttcttcagca ataacttctc cgcccttttc aacgaaggtc
     1141 tttttgaagt attccgccag tcctttgccg taatcactgg aagtatcccc atagataacg
     1201 gccttcttgg ctttcagatt gtcagcggca aactgcgcca tcaaagtgcc ctggaaagga
     1261 tcgatgaagc agacccggaa gatgtaatct ctggtcgcgc cggttttgtc gtcaacggtt
     1321 acattggcgg ccgttgctgc cggagcaatc agcggaattt tgttgtccat catcaccggg
     1381 gtgctgccgg ctacgttgcc gctggtcaac ggtccgatct gggcgacgat tttttcgcct
     1441 accaatgcgg cacttacgct catggcttcg tcagccactg atttgcagtc tctttcaatg
     1501 ggattaagct gcttgccaag cacgccgccg gcctggttga tctcctcgat ggcgagaatg
     1561 gcaccgtctc ttgcgttggt gccgtagctg gcaacaccgc cggacagctc aacattgaca
     1621 ccgacattga ttttctcggc ttgttctgtg gttttgtcgc cggttccgcc gccgccgcag
     1681 ccagccacga tagcgagcat gaataccatc aataccagca tcgctactct tttactgctc
     1741 tttttcatcc tgttcctcct ctttctttct ttttttatca ctatcacggc cgaccgtgaa
     1801 agttcccatt taagtctgtt tcctgacgaa tattccggac gaaaaatagt aaacgtcgtt
     1861 tcaagaaatt gtagtacttc ccgagcattt gttcaagtct aaacctgcaa aagaacggca
     1921 ggattttcga atttaaaccc ggccggaagt ttaatgtatc ctgtgtgatt cgtcaaaaac
     1981 cgtgcatctt cctgctgttt tttcggtatt tttttgtttt tctgtatcta aaagcttttc
     2041 gcttcagcaa tgaaaagaag aagggagtgc cgatcccctt taaaagatgt gtggggtgtc
     2101 accaactcct tcccctgtca cttcctttaa gccttgatcc gcatgtctcc aacccttctc
     2161 gtaaagcgaa ttctatcata atattctacc aaaggaagcg catactttcg agaggtatta
     2221 aaaatatccc gggcggtggc cagtgaaagt tccttttctt tggtgaaata ttccgccagc
     2281 agtgccttgc ctttttccat ggctgctgtg gaaaaataga tattgtcgct gattttcagc
     2341 aaatagccgc tgtctattaa ataggaaata atttcattgc gctgggcttc ctccaaaccc
     2401 ttcaaaacat cgatttcatc ccaaccgggc gggttaaatt cattttcctg aaacttttgc
     2461 ttgatttccg caataatttt ctgttcttta tctccgggat gggcaacata attcggtaag
     2521 gtaataagat tgttctgact ttgcaacacg ccgtggtctt cataatattt taacaagccg
     2581 ctgaagacct tgggatttaa ttggttgaac ccgcggctgc gcatatcttc tttgggataa
     2641 ccctggcgca gtggatactt ttgcagatgt tccgcaatcg tcttgctgat ggataggccc
     2701 atggcctgta ctgcctgggc gctgaaataa taggctccct tgccgctggt atcgatgatt
     2761 ttctcatcat ccagcagttg ttggacttcg gtttcgacat tggcctcagt attgccggtg
     2821 cgacgggcaa tttcctggat cgtcagcggt tccgtaccgg atgtttccag ttcgtgcagc
     2881 accaaatcat aaagactgcc ttcttctttg accatcagat cgttgaggac ctcttccttg
     2941 aaacgctttt gtcgggtagc attgggatcg atgatggtcc ctccgccgat ggtattaacc
     3001 ggggaataat accgaattac aaagggatca cccttgtagg caaccacttg tttttccatc
     3061 acaatctggg catacgcttc gtctcccggc aggagttcat cccgatccag cagaacaatt
     3121 ctgcccagcg tctcatccgt gcccaaatga aatctaatgc ggttccagtt ctgcagcgtg
     3181 cgcgggctgc tggagagcag acgcaagcgg gcgtttaccc ggtaactggg ttccaaatag
     3241 ccgctgctgg ccagcagata acctcttttg atatcggcca gatcaacgcc ctgcaaattg
     3301 accgcgactc tctgtccggc ataggcagtg ggaacttttt cattatgcac ctgtaacgtt
     3361 cggactttga ccatgcgctg caccggcatc agctccagac tgtctcccac attaatctgc
     3421 cctgaccaca aggtgccggt caccaccgtt ccaaaaccgg caatgctgaa gacccggtcg
     3481 atgggcagac gagcctgtcc gaaaaccggc ttttctacca cgtcggcggc aacttcctca
     3541 atggtcgtca gcaattcctt gatgccctcc ccggttaccg cggatacggc gacgatgggg
     3601 cagcctttca ggatgccttg ggcagtatac tcgctgactt cttcttccac catcatcagc
     3661 cattcttcat ccaccatgtc ttttttggta atgacgatga tgcccttgtc cacccctaat
     3721 aactcgatga tgtccatatg ttcacgggtc tggggcatga cgccttcatc ggcggcgatg
     3781 gtgaacagaa ccatgtcaat gccaaaggct ccagccaaca tatgacggat aaatctttca
     3841 tgcccgggga cgtctacgat ggcggctttc tgcccgctgg gcagggaaaa aggcgcaaat
     3901 cccaattcaa tggatatgcc tcgttgcttt tcttctttca gccggtcggt ttcaaagccg
     3961 gtcaaggctt tgaccagggt ggtcttgcca tgatcgatgt gtccggctgt tccgataatc
     4021 agtcttttca ttctttttcc tcctgaccca acagggggac gttttttttg ttggctttct
     4081 cagttgtcaa tcaaccaaca aaaggaacgt ccccttgttg gcccgtactg gcggttattc
     4141 attgcagcac ttgggccagc agcccggcga cttcttcctg gtctccgggc agcagggtac
     4201 gtacgctgag caacagtgca tcttcctgtc gacgggtcag taaggccggt tccagcatgc
     4261 gcaacgctcg ggcagcagct tccaggctct tgccgcgcaa ggccagttgg actccgtaac
     4321 cgggcagttt gtacgttggg taggcgccgc ctccgaccat gtcctccagt gcgatgactt
     4381 ccagtttgct gacctgggga ttgccgtcca gtgtctgctc cagcttttcc ctgagttctt
     4441 cggcattttc ccgcaattgc tcgagaggca gggtcagcat ccgaatgacc ggtatatgct
     4501 gacggggatc tccgctcaaa tattccagca gcgtcccttc cagggcagca atggttagtt
     4561 tgtccactcg cagggcgcgg gtcagctgat tctttttcat ggcttcaata tgtttttgct
     4621 ttccgacgat aatgcctgcc tggggaccgc ccaacagctt gtctccgctg aaagtaatga
     4681 tatccgcgcc ggaattgatg ctgtccggga cggtgggttc atccttaagt ccccattctc
     4741 ctaaatccac caggatgccg ctgcccagat catgcatcac cggcaagcct cgggcctgcc
     4801 ccaaagcggc tagttcatcc aaccctgctt ccgcagtgaa gcccatgatt ttgtaattgg
     4861 acgtgtgggc agaaaacaac aaggcggtat cctcggtgat ggccgcttcg taatcacgca
     4921 aataggtgcg gttggtggtg ccgacttcaa ccagtttggc cccgctggct ttcattactt
     4981 cgggaatgcg aaaggccccg ccgatctcga tcaattgtcc ccgggaaacg ataacttcac
     5041 gaccttgggc catagttgac agccccagta aaacggcagc ggcgttgttg ttgacgacca
     5101 gcgcagcctg cgcaccggtc agccgtacca gcagttcctc aacgtgcgta tagcgcgagc
     5161 cgcggtgccc ttccgccaga tcaaactcaa gatcgttgta gcgctgcgct gtggcctgca
     5221 tatattgaaa tgcccgatcc gccagcggcg ctcgtcccaa attggtatgc aaaacaacac
     5281 ccgtggcgtt gatgacccgg ttcaggctgc cgcggacagt attttttaag aacacatccg
     5341 ccttttgcaa aaccaattgg ctgatttctg ccttggcaat cgtttgccgg gatgtccgca
     5401 actccgtgcg tacatcgctg accgcccgac gcagtatatc aacaatgaat tcacgctgat
     5461 acactgccag caggtgccgg gtttccggct gggccagcaa ctcgtctatc gatggaatgt
     5521 ttctcagatt ggtcatcaga gctttcctcc catga
//
LOCUS       DAOTHZ010000107         5544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_17407_length_5544_cov_7.888869, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000107 DAOTHZ010000000
VERSION     DAOTHZ010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17407_length_5544_cov_7.888869"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="P5549_09570"
     CDS             <1..921
                     /locus_tag="P5549_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRW13359.1"
                     /translation="EELLCSSERAKDLAAEFVRLAVPVSFGAVVLPLVQVLDAIIVPG
                     RLMAINYTTSQATALYGQLSGMAAVLISLPTIFTISIATSLVPAVSEALANRDHRSLN
                     DRINSGIRAGMLISLPCAAGLYVLAVQICDLLYAAPEAGIPLEILAFSTIVLAAFQLS
                     SAGLQGIGRPEIAMRSLLVTGLLKVVFNYSLTSVPMLNIRGAAIGTVLAFGIGSMLNL
                     FFLKRYTGVKFETGRLLKLSLITVTMGVCVKMSYSTVVGIGLHSHWATLLAIVIGVAV
                     YAALLLLFKELDMQMIKKLIGRGEQTGPPL"
     gene            940..1710
                     /gene="mazG"
                     /locus_tag="P5549_09575"
     CDS             940..1710
                     /gene="mazG"
                     /locus_tag="P5549_09575"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRW13360.1"
                     /translation="MMSSNGQALEELIAIMDRLLGEGGCPWDREQTHASLVRYLIEET
                     YEVIDAINHGDMDELATELGDLLLQIVFHAALAEREGHFQFADVARRVSRKMVDRHPH
                     VFGTMQLETAEDVLNKWEGFKKKEGKKYLLDGIPQGLPALMRAEKMQEKAARVGFDWP
                     DVQGALVKFQEEVEELVRADNAKDKLEETGDLFFALVNIARLEGVEPEQALQSSNNKF
                     ARRFHYIEKRLEDSNQEFTDMSLEELDLLWEEAKSQGL"
     gene            1845..2117
                     /locus_tag="P5549_09580"
     CDS             1845..2117
                     /locus_tag="P5549_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRW13361.1"
                     /translation="MNKTELISEVAQKANITKKDADKIVNAFFATVESALKAGDKVQL
                     VGFGTFEVRERQARKGRNPQTGEEISIPSTKVPAFKAGKALKDAVD"
     gene            2119..2361
                     /locus_tag="P5549_09585"
     CDS             2119..2361
                     /locus_tag="P5549_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRW13362.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDFAENERVMVNGRVAKPSTEIKKG
                     DRITLRIGERETVFEVLDIKENIKASEARNLYKIIE"
     gene            2568..2837
                     /gene="yabP"
                     /locus_tag="P5549_09590"
     CDS             2568..2837
                     /gene="yabP"
                     /locus_tag="P5549_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HRW13363.1"
                     /translation="MNEHSIMMANRKSVELSGITNVINFDEEEIILETVMGYLEIGGE
                     ELHITMLNLDKGQVAVVGSINQLAYKPQGTNLKNKGKTILNRLLK"
     gene            2849..3283
                     /locus_tag="P5549_09595"
     CDS             2849..3283
                     /locus_tag="P5549_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HRW13364.1"
                     /translation="MLGLFTQIQILVFTLLLGVIAGFVLHFYQLVIKAWKAGKHILYL
                     LDFVFWVLMILIVSLGMLFINQGALRFYTFVFLVLGALFYYKTLAPYLYQPMQTMAAA
                     GVHFATLMAGWIRRPGLWLIQKIRTKWRQHHPPISPDESPRE"
     gene            3354..3584
                     /locus_tag="P5549_09600"
     CDS             3354..3584
                     /locus_tag="P5549_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HRW13365.1"
                     /translation="MKIEIRGAEKLSFRERQVVVLKEMGKSTEEITRMLKVSASTVAT
                     LYNRARSKGYEVVIVLPGDVMGIFAEEDVDEA"
     gene            3574..3852
                     /locus_tag="P5549_09605"
     CDS             3574..3852
                     /locus_tag="P5549_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRW13366.1"
                     /translation="MKPENKRRRLLMSGLICIVLLVTIVPRGKTIMELSAQKRELKNE
                     KAQLQEINRQYQDKLRDLKAPETIEKLARERLGMVKNGEKVIIDLQED"
     gene            3930..4382
                     /locus_tag="P5549_09610"
     CDS             3930..4382
                     /locus_tag="P5549_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRW13367.1"
                     /translation="MPTEQTNIVPGEIIEGKVQGITTFGAFIELPGGVVGLVHISEIA
                     DTYVKDVKDYIKEGDQVKVKVLNIDGKKIGLSIKQALPPKVVPPVPSQPKYNRSEPRR
                     DNRGFSGPKKPEAPVTLDDKISRFLKESDERMQPLKNKVEPRRRKNYQ"
     gene            4464..5333
                     /locus_tag="P5549_09615"
     CDS             4464..5333
                     /locus_tag="P5549_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HRW13368.1"
                     /translation="MRCAAIDIGTNSCRLLIAAREGQRLETLYKEIETTRIGAGLSQS
                     GQISGEAAQRSWQWLEKIEPILRAYDVQYARAVATSAVREAGNGQAFVEEAVRRSHMS
                     VEIISGEEEAKLSYIGVEKGLQLDHPPLVADLGGGSTEFICNHQDFVISLPVGAVRAT
                     EMQMNAADIAERLQPVQAMQGRFAEYPLVMVGGTASTIASVKLGLDEYDSKKVHGHQV
                     TRQEAADIYQLLERTPLPLRKRLPGLQPERADIINAGVLITMLIMELLGKSSILVSDS
                     DLLQGIIWSQEYL"
BASE COUNT         1614 a         1098 c         1466 g         1366 t
ORIGIN      
        1 gaagagcttc tctgcagttc tgaacgggcc aaggatcttg ctgctgaatt tgttcggctg
       61 gctgttccgg tgtcctttgg cgccgttgtg ttgccgctgg tgcaggttct tgatgccatt
      121 atcgtaccgg gacgtttaat ggccatcaat tataccacct ctcaggcgac agccctgtac
      181 ggtcaactat caggaatggc agccgtattg attagcctgc ctacgatttt caccatctct
      241 atcgctacca gtttggtgcc ggcagtgtcg gaagcactgg ccaaccggga tcaccgcagt
      301 ttaaatgacc gcattaattc cggtataaga gccgggatgc tgatctcgct gccctgtgct
      361 gccggtttgt atgtactggc agtacaaatc tgtgatcttt tgtatgctgc tcccgaagcc
      421 ggaataccgc tggagatatt ggccttctca accattgtgt tggctgcctt ccagttgtcc
      481 agcgccggac tgcaggggat tggacgaccg gagattgcca tgcgcagcct gctggtaacc
      541 ggtttgctca aagtggtttt taactacagt ttaaccagcg ttccaatgct caatatcaga
      601 ggcgcagcga ttgggacggt tttggctttt gggattggat cgatgctgaa cttgtttttc
      661 ctgaaacgat acacaggggt aaaatttgaa accggccgcc tgctgaaatt atctctgatt
      721 actgttacca tgggtgtttg tgtcaagatg agttacagca ctgtggtggg aatcggtctt
      781 cattcccatt gggctacttt gctggccatc gttattgggg tagcagtgta tgctgcatta
      841 ctgctgcttt ttaaagaact ggatatgcag atgatcaaaa aattaatagg tcggggtgag
      901 cagacaggtc cccctttgta aagacggagg agaagaaaaa tgatgagcag taacgggcaa
      961 gccttggaag aactgattgc tattatggac cggcttctgg gagaaggcgg gtgtccctgg
     1021 gatcgggaac agacccatgc atccctggtg cgctacttaa ttgaagaaac gtatgaagtg
     1081 attgatgcca ttaaccatgg cgatatggat gaattagcga cagagttggg ggatttgctt
     1141 ttacaaattg tatttcatgc tgcgctggct gaacgggaag gacattttca atttgctgat
     1201 gtggcccgac gtgtcagccg taagatggtg gacaggcatc cccatgtttt tggcaccatg
     1261 caactggaaa ctgccgagga tgtgttaaat aaatgggagg gctttaaaaa gaaagaaggc
     1321 aaaaaatatt tgcttgatgg cataccccag ggtttgccgg ctttaatgag ggccgaaaaa
     1381 atgcaggaaa aagcagcccg ggtaggtttt gactggccgg atgtacaggg tgcgctggtt
     1441 aaatttcagg aagaagtaga ggaattggta agagctgata atgcgaagga caagcttgag
     1501 gaaaccgggg atctgttttt cgcactggtc aatattgcca gactggaagg cgtcgaaccg
     1561 gaacaagccc tacagtcatc caacaataaa ttcgcccggc gctttcatta tatcgaaaaa
     1621 agattggaag acagcaacca ggaatttact gacatgagcc tggaagaact tgatctactc
     1681 tgggaagaag ccaaatccca agggctataa gtcatggaca gcttgccgat gaacatgctg
     1741 cgagaatcaa ctttacaact tttacatcga taaaaggagg ataaacaggc tcggcataga
     1801 atagcataat atgccaataa tatactacag aaggagggat atcattgaac aagaccgaat
     1861 taatcagtga ggttgcccag aaggccaaca taactaaaaa ggatgcagac aaaatcgtca
     1921 atgctttctt tgccaccgtt gaaagcgcat taaaagctgg tgataaagta cagctcgttg
     1981 ggtttggcac ctttgaagta agggaacgtc aggccagaaa aggcagaaac ccgcagaccg
     2041 gggaggaaat cagcattcct tccaccaaag taccggcctt taaagcgggc aaggcattaa
     2101 aagatgccgt agattagaat gcgtctggat aagtttctaa aggtatccag gattatcaaa
     2161 cggcggaccg tagccaagga ttttgccgaa aatgaaagag tgatggtgaa cggcagagta
     2221 gccaagccat caaccgaaat caaaaaggga gacagaatca ctttacggat tggggagcgg
     2281 gaaaccgttt ttgaggtact tgatatcaag gagaacatca aagccagtga agcgcgcaac
     2341 ctatacaaga taatcgaatg aatggaacgt ctcgtatgta aatacgggac gtattttttt
     2401 tttgaccgtt ttggccaggc gaagacactg tccaaggcta aacataaaat gaggagaacc
     2461 agtttgtgaa taaaatggtt acggtttgca tagctattgg caaagcagat ttcatcatct
     2521 gcatagcata agcggatgat ggagcgctca tgaaggaggt gagatcaatg aacgagcatt
     2581 ccattatgat ggcgaatcgc aaatcagttg aattaagcgg aatcaccaac gtaatcaatt
     2641 ttgatgaaga agaaattatt ctcgaaacag tgatgggtta tctggagata ggaggagagg
     2701 aactgcatat caccatgctc aatcttgaca aaggacaggt ggcggtcgtg ggaagcatca
     2761 accaactggc ttacaaaccc caggggacaa atctcaaaaa caaaggaaaa acgatcctta
     2821 accgactctt gaagtagggg ggattccatt gctgggtctg tttacccaga tacaaatact
     2881 tgttttcact ttgctgttgg gcgtaatcgc cggatttgtt ctgcattttt atcaactagt
     2941 catcaaagcc tggaaagctg gcaagcatat cttgtatttg ctggactttg ttttctgggt
     3001 attgatgatc ctgatcgtat cgctgggcat gctgttcatc aatcaaggcg ctttgcggtt
     3061 ttatacattt gttttcttag ttttgggagc actattctat tataaaacat tggcgcccta
     3121 tttatatcag cctatgcaaa ccatggccgc agcaggggta catttcgcta ccttgatggc
     3181 tggttggatc cgccgccctg gattatggct gattcaaaaa atccgcacga aatggcgtca
     3241 gcatcatccc ccgatttccc ctgacgaatc cccccgtgaa taaaccaaaa aaacgcaaaa
     3301 aaagttttgt ccacatattg tggaaaatat gtggatgagt tatggttaat aatatgaaga
     3361 tcgaaatcag gggagccgaa aagcttagtt tcagggaaag acaagtcgtg gtactgaaag
     3421 aaatgggcaa aagcaccgaa gagattaccc gtatgctcaa agtgagcgcc agtacagtgg
     3481 ctactttata taatcgtgcc cgcagcaagg gatacgaagt tgtcatcgtg cttcccggag
     3541 atgtgatggg tatttttgca gaggaggacg tggatgaagc ctgagaataa aagaaggcgg
     3601 ttgttgatga gcggcttgat ttgcatcgtc ttgctcgtaa ccatcgtgcc gcgggggaaa
     3661 acaatcatgg agctgtctgc ccaaaagcgg gaattaaaaa atgagaaagc ccagctgcaa
     3721 gaaatcaatc gccaatacca ggataaactc cgggatttaa aagcacctga aaccatcgaa
     3781 aaactcgccc gggagagact gggaatggtg aagaacggtg aaaaagtaat cattgatcta
     3841 caggaggatt aatcgctgtt tctggttgac tagccccgat gcttttaata caatgaacat
     3901 agtattattt gagggggatc ttaattttta tgccaactga acagacgaat attgtgcctg
     3961 gcgagattat tgagggcaaa gtacaaggga ttacaacgtt tggagcattc attgagttgc
     4021 ccggtggcgt ggtaggtttg gttcacattt cggaaatcgc agacacttat gtgaaggatg
     4081 tcaaagacta catcaaagag ggtgaccagg tcaaggtaaa agtgctgaat atagacggca
     4141 aaaagattgg actttctatt aagcaggcac ttccacccaa agttgttccg ccagtaccca
     4201 gtcaacccaa atacaatcgt tctgaaccgc gcagggacaa tagagggttc agcgggccga
     4261 aaaaacctga agcaccagtt acgctggatg acaaaatcag caggtttcta aaagaaagcg
     4321 atgaaagaat gcagcccctc aagaataaag ttgagcctcg cagaagaaag aactatcagt
     4381 aaacagatat gcaaagcact tctctaaacg aagtgctttt ttgtgcagtc aatcattcaa
     4441 caagaataaa taggaggttt gttatgaggt gtgcggccat tgatattggc accaattctt
     4501 gtcggctgct gatcgctgcc agagagggtc agaggttgga gactttgtac aaggaaatcg
     4561 aaaccacacg gatcggggcg ggtttaagtc aaagcggaca gatcagcgga gaggcagctc
     4621 agcgtagttg gcagtggctg gagaagatcg agccgatctt gcgggcatat gatgttcaat
     4681 atgctcgcgc agttgctacc agcgcggttc gcgaggcagg caacggacaa gcgtttgtgg
     4741 aagaagccgt ccgccgcagt catatgtcgg tggaaatcat tagcggggaa gaggaagcaa
     4801 agctgagtta tatcggcgtt gaaaaaggtc tgcagctgga tcacccaccg ttggtcgccg
     4861 acttgggggg cggcagtacg gaattcatat gcaatcatca ggattttgtc atttctctgc
     4921 cggtgggtgc ggttcgagcc acagaaatgc agatgaatgc tgctgacatt gccgagcgtt
     4981 tgcaaccggt ccaagccatg caaggtcgat ttgcggaata tcctctggtg atggtaggcg
     5041 gaacagccag tacgattgca tccgttaaat tgggtctgga tgaatatgat tcgaaaaagg
     5101 tacacgggca tcaggtgaca cgtcaggaag cagcagatat ttatcaactg ctggaaagaa
     5161 ctccgttgcc gctgcgtaaa agactgccgg gtttacagcc ggaaagggcc gatattatta
     5221 atgccggggt tcttattacc atgctgatca tggagttgct gggaaaatcg tcaatccttg
     5281 tcagcgacag cgatttattg caagggatca tttggtcgca agaatattta taaacaaaat
     5341 aatatattaa gcaggattta tttgcttttc gtagaatgat taacgagtat gtaaaaagtt
     5401 atgattttca aaaaaagatt gttgaaattc ataactgaaa cgtcgggaaa aaataaagga
     5461 aaggggaatt gtaatgggat ttttaacacc tgcagagatt gcggcaaccg catgtgcagc
     5521 aggcaaatcc aaggctgaac tgcc
//
LOCUS       DAOTHZ010000108         5523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_17488_length_5523_cov_6.792246, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000108 DAOTHZ010000000
VERSION     DAOTHZ010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17488_length_5523_cov_6.792246"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..712)
                     /locus_tag="P5549_09620"
     CDS             complement(362..712)
                     /locus_tag="P5549_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13369.1"
                     /translation="MRVRIIETIEQWHILKAALIEKGYKLWQTQFSYDAPEGYRAWFW
                     KDDKQVEVATHNQEIQSDIVSCRYLKSYTNKDNQKNNCEIGFTSKDEEQEWTLAAKTI
                     LKNNRNAFDRLGKI"
     gene            complement(997..1668)
                     /locus_tag="P5549_09625"
     CDS             complement(997..1668)
                     /locus_tag="P5549_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HRW13370.1"
                     /translation="MAIFREQKRAGRDRSAEDRRRHRQLVEEHIKRNLGNIIAEESII
                     GQSKGKTFKVPIRGLKEYQFIYGDNHPGVAGGKGDEKRGQRITETNRQVPGSGDGAGS
                     IEGEEIYETEITLEELVEYLFEDLHLPFMERKKFSLLETETTNKRRGIQRKGIPPRLS
                     KKRSMMERIKRMKSAQRVNEGENENQVLQPGERFPFSEYDLRYQRLKEQTKPESNAVV
                     ICTLL"
     gene            complement(1678..3603)
                     /locus_tag="P5549_09630"
     CDS             complement(1678..3603)
                     /locus_tag="P5549_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HRW13371.1"
                     /translation="MNSHFHSLIQKDRQEKRHESWEGTLLDYLEVVKEKPQLTMLAHE
                     RMYEIIAAAGVDTIKTEDHPRLRRIYGNDIIKRYQFFEQDFFGIDKVIMEIVRYFHSA
                     TMRGEESRQVLYLVGPVGSGKSSIMEALKRALEKSPPIYTLKDCPMREEPLHLIPKHL
                     RPDFEELLGAKIEGDLCPICRYRLINEYDGEYERFPVRTMGFSIRGRKGIAVVPPVDP
                     NNQDASVLVGSVDISKMDLYPEDDPRVLSLNGAFNAGNRGLVEFIEIFKNDVEYLHTI
                     ITATQEKSIPSPGKGPMIYFDGIILAHSNEAEWNKFKSDHTNEAILDRIVKIEVPYCL
                     ELDQEVKIYQKILKMSKFKAHIAPHTLEIASMFAVMSRLVPSNKVDSITKMRIYNGEE
                     IVVKDSSQRVDIQELRDEAGKEGLTGISTRFIMKAIDNALSESECDCINPILVMEALV
                     KAIRATDMSDEDKKKLLVLLQETLKQEYHRILEKEVTRAFIHGYREQAETLFNNYLDH
                     AEAYVNKTRIKDKNTGEELDVDEKFLESIESQLGISGTAAKGFRNDVTAYMFSLLRNQ
                     VKIEFGSYEPLKEAIEKKLINSVRELSRVVTQARVRDQEQKQKYDAMVEAMRDNGYCE
                     HCCNVILKYAANNLWKD"
     gene            complement(3860..5083)
                     /locus_tag="P5549_09635"
     CDS             complement(3860..5083)
                     /locus_tag="P5549_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRW13372.1"
                     /translation="MFTKFYYYLKHFGVPVSINEWQALMEALDMGLAGSSLTGFYQLC
                     RALLVKTEAHYDRFDLAFASYFGDIETPEGLPDKIWEWLNKELPQIEITDEMRRNHMQ
                     IDLDEMKRMLEERLREQTEEHNGGNRWVGTGGTSPFGHSGYHPGGIRVGGDSRNQSAV
                     KVAGMRRFQEFRTDETLGIRQFQIALRKLRQFTTRLDGVKDELDLDGTIDKTSQNAGR
                     LELVWERPRVNGMKVLLLMDAGGSMISYSHLCNQLFTAVNKSNHFKDLKVFYFHNCIY
                     DWLYHDSSCSQSEYTDTEYVLRTYSKDYRVIIVGDASMAPYELMRVGGVIDWDLHNDQ
                     PGFEWLKKIRKQFEYSVWLNPIPAKYWDWMDGSYTIKTISSVFPMEELTVEGLERGIR
                     RLKSKQSTGAALSVK"
     gene            complement(5156..>5523)
                     /locus_tag="P5549_09640"
     CDS             complement(5156..>5523)
                     /locus_tag="P5549_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRW13373.1"
                     /translation="PDAFLRRCIFHYIAFPEPDAMEQIIRVHHPHVERKLLEQAMETF
                     YMLRNIPNIQKKPSTSELIDWLQALVVGGISPNKIKQELPFLGVLLKKNEDLDTLLNQ
                     LYNKNQNKAQPRMGGSYYR"
BASE COUNT         1326 a         1391 c         1193 g         1613 t
ORIGIN      
        1 ttggtgggcg tctctttttt caaagaccgc ttttaaggat tacaggaatg ctcctaattt
       61 gggaatatct ttgtatatga atatatttaa tagaccaaat catcagactt tgttttaaaa
      121 ctgcacgcaa taaaaaatat ataataatta tttctgctat cgacaaaacc ccatagactg
      181 ctagtatgtc ttgtttaaaa attcagagat ttaaattgag taaactcatt ttcacacatc
      241 cgatcccccc ccgatgtgct gcaactgcga tataatgagg catccataaa ttcaaatttg
      301 cacccttaca aaaatatata ttaaagctcc aaaattatga aggacagagg aatggaaagc
      361 attatatttt tccgagtcgg tcaaaagcat tcctattatt tttcagaatg gttttggcgg
      421 caagtgtcca ttcttgttcc tcatctttag aagtgaatcc tatctcacaa ttattctttt
      481 ggttatcttt attagtatat gattttaaat atctgcagct aacaatatca ctttgtattt
      541 cttggttatg cgtggctact tccacctgct tatcatcttt ccagaaccat gcgcgatagc
      601 cttctggggc atcatatgaa aattgcgtct gccatagttt atatcctttt tctatcaaag
      661 cggctttaag tatgtgccat tgttcaatcg tttctataat tctaactctc atatcatagc
      721 acctttttca gaacttttgt tctttataat atcatcgttc tagctgatat gcaattgatt
      781 ttccccaaaa tgtatggatt ttaacatttc gtataaatcc gttttcgaaa aatagagttc
      841 acatccttta aactctgatc aacattaaat ctctctactt taatatgtaa tcgcaaagac
      901 atacaccttt aaaaaccact aaataaaaac caattattac gataaagcta atttataaca
      961 acctcccttt aattttctct taataacgcc tactggtcac aaaagggtgc agatcaccac
     1021 cgcattggac tccggcttgg tctgctcctt gagacgctga tagcgcaggt catattcact
     1081 gaagggaaaa cgctcccccg gctggaggac ttggttttca ttttcaccct cattgactcg
     1141 ctgggctgat ttcattcttt taatccgttc catcatggaa cgctttttgg aaagccgcgg
     1201 cgggatccct ttacgttgga ttccccggcg cttgttggtg gtttccgttt ccagcaggga
     1261 aaatttcttg cgttccataa aaggcagatg aagatcctcg aagagatatt ctaccagttc
     1321 ttccagggtg atttccgttt cgtagatctc ctcaccttcg atgctgccgg ctccgtcccc
     1381 agacccgggc acctgccggt tggtctcggt aattctctgc ccgcgtttct catcaccctt
     1441 gccgccggcc accccgggat ggttgtctcc gtatataaac tggtattcct ttaatccccg
     1501 gatagggacc ttgaaggttt tccccttgct ctggccgata atgctctcct cggcaatgat
     1561 attgccgagg ttgcgtttga tatgttcctc caccaactgg cggtgacggc ggcggtcttc
     1621 cgcactgcga tcccggccgg cacgcttttg ctcccgaaat atcgccatta cttcacccta
     1681 atccttccac aggttattgg cagcatattt gaggatgacg ttgcagcaat gctcacagta
     1741 accattgtcc cgcatcgctt ccaccatggc gtcatacttc tgtttctgtt cctggtcacg
     1801 cacccgggcc tgggtaacca ccctggacag ttcccgtacc gagttgatca atttcttttc
     1861 gatggcctct ttgagcggtt cataactgcc aaattcgatc ttgacctgat tgcgcagcag
     1921 ggaaaacata taagcggtga catcattgcg gaatcccttg gcagccgtcc cgctaatccc
     1981 cagctggctt tcgatagatt ccaggaattt ttcatcgaca tccagttctt cgccggtgtt
     2041 tttgtctttg atccgggttt tgttgacata ggcttcggcg tgatccagat aattgttgaa
     2101 gagcgtctcg gcttgttccc gatagccatg aatgaatgcc cgggtcactt ctttttccaa
     2161 aatacgatga tactcctgct tcaaggtctc ctgcagcaag accagcagtt tcttcttgtc
     2221 ttcatcactc atatcggtag cccgaatggc cttgaccagg gcctccatca ccaaaatcgg
     2281 attgatgcag tcacattcag attcagacaa agcgttgtcg atggctttca taataaaacg
     2341 agtggatatc ccggtcagtc cctccttgcc ggcttcatca cgcaattcct gaatgtctac
     2401 tcgctggctg gaatccttga ccacgatttc ttccccgttg tagatgcgca ttttggtaat
     2461 gctgtccacc ttgttggagg ggaccagccg gctcatcact gcgaacatgg aagcgatctc
     2521 cagtgtatgg ggggcgatat gagccttgaa tttactcatt ttcaagatct tttgatagat
     2581 ctttacttcc tgatccaact ccagacaata gggaacttcg atcttgacga tccggtccag
     2641 gatagcctcg ttggtatggt cggatttgaa tttgttccac tcggcctcgt tggaatgggc
     2701 cagaatgatc ccatcaaaat agatcatggg gcctttgccg ggggatggga tggatttctc
     2761 ctgggtggcc gtgatgatgg tatgcagata ttcaacatcg tttttaaaga tttcgataaa
     2821 ctcgaccagt ccccggttgc cggcattaaa tgctccgttt agggagagaa cccgggggtc
     2881 gtcttcggga taaagatcca ttttggaaat atccaccgag ccgaccaata ccgaggcatc
     2941 ctggttattg gggtcaaccg gcggcacgac cgctatgcct ttgcggccgc gaatggaaaa
     3001 acccattgtc cttactggaa accgttcgta ctctccgtca tattcattga tcaagcggta
     3061 gcggcaaata gggcacaaat ccccttcgat tttcgcgccc agcagttctt caaaatccgg
     3121 ccgcaaatgt ttggggataa gatgcaaagg ttcttcccgc atggggcagt ccttcagggt
     3181 ataaatggga gggctctttt ccaaggcccg cttcaaagct tccataatcg aggatttccc
     3241 tgagcctacc gggcctacca gatacagaac ctgacgggat tcctcgcccc gcatggtagc
     3301 cgaatgaaag tagcgcacga tctccataat caccttgtcg atgccaaaaa agtcctgctc
     3361 aaaaaattga tagcgcttga tgatatcatt gccatatata cgccgcagtc gggggtgatc
     3421 ttccgtttta atggtatcca caccggcggc ggcaatgatt tcatacattc tttcatgggc
     3481 cagcattgtc aactgcggtt tttctttgac tacctccaga taatccagga gggttccctc
     3541 ccagctttca tgccttttct cctgtcgatc cttttgtata agcgaatgga agtgactgtt
     3601 caaccatatc cctcctttat tcatgccagc agcaccagat gcaaaactaa aggataatat
     3661 tatatatatg cagggaattg gaaattttgt gcgttgcctg gattttatta ttggcctcat
     3721 aaaaaaaccg gtggtttaaa ccaccggtgc agtcaccagg ctctgttgac ccggaaagcc
     3781 ggttgagcta ttgcgctgac aacagaaaaa agacgcccct gtcaattgga cgtcttggca
     3841 ttgctgatcg gttattgact tattttacac tgagtgctgc accggtcgac tgcttgctct
     3901 ttaatcggcg aatgcctctt tccagccctt ccacggtcag ctcttccatg ggaaataccg
     3961 agctgatggt cttgatggtg tatgatccgt ccatccagtc ccagtacttg gcggggatcg
     4021 ggttgagcca aacggaatac tcaaattgtt tgcggatctt ctttaaccac tcaaacccgg
     4081 gctggtcatt gtgcagatcc caatcgatca ccccgccgac ccgcattaat tcatacgggg
     4141 ccatgctggc atcaccgaca atgatcaccc gatagtcctt actgtaggta cgcagcacat
     4201 attcggtatc ggtatattca ctctggctgc aactggaatc atgatacagc caatcgtaga
     4261 tacaattatg gaaataaaat accttcagat ctttaaaatg attggattta ttgactgcgg
     4321 taaacaactg attgcagagg tggctgtagg aaatcattga tccgcctgca tccatcagca
     4381 gcagcacttt catgccattc acccgggggc gctcccaaac cagttccaaa cgtcctgcgt
     4441 tttgactggt cttgtcaatg gtcccgtcca gatccaattc atccttgacc ccgtccaggc
     4501 gggtggtaaa ctggcgcagt ttgcgcagtg caatctgaaa ttgtctgatc cccagcgtct
     4561 catcggttct gaattcctga aatcttctca tgcctgctac tttaacggct gactgattgc
     4621 gggaatctcc tccgaccctg attccgccgg gatgatagcc ggaatggccg aaaggagagg
     4681 ttccgcccgt gcctacccag cgattgccgc cgttgtgttc ttcggtctgc tcccgcagtc
     4741 tttcctccaa catgcgtttc atctcatcca agtcgatctg catgtggttg cgtctcatct
     4801 catcggtaat ttctatctgc ggcagttcct tgttgagcca ttcccaaatc ttgtcgggca
     4861 atccttccgg ggtttcgata tccccaaaat agctggcaaa ggccagatca aaacggtcat
     4921 aatgggcttc ggtcttaacc agcagggccc ggcaaagctg ataaaatccg gtcagactgg
     4981 aaccagccag acccatgtcc aaggcttcca tcaaggcttg ccattcattg atcgatacgg
     5041 gcacgccgaa atgtttcaga tagtaataaa attttgtaaa catatgttgt ccctctatat
     5101 gcagaattca tccattgcct gtcaaacggt ctgcaccggc gggcaggcag gtcatttaac
     5161 gataatagct gccgcccatg cggggctggg ctttattctg gtttttattg taaagctgat
     5221 tgagcaaagt atccaaatct tcatttttct ttaacaaaac tcccaggaag ggcaattcct
     5281 gtttaatctt gttggggctg ataccgccca ccaccagagc ctgcagccag tcgatcagtt
     5341 cgctggtgct gggcttcttc tggatattgg ggatgtttct cagcatgtaa aacgtttcca
     5401 tagcctgttc cagaagtttt ctttctacat gggggtgatg gacacgaatg atttgctcca
     5461 tggcatccgg ctcaggaaaa gcaatgtaat ggaaaataca tcttctcagg aaagcatccg
     5521 gca
//
LOCUS       DAOTHZ010000109         5376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_18099_length_5376_cov_7.329449, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000109 DAOTHZ010000000
VERSION     DAOTHZ010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18099_length_5376_cov_7.329449"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..1963
                     /locus_tag="P5549_09645"
     CDS             509..1963
                     /locus_tag="P5549_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW13374.1"
                     /translation="MTINILKEPDVTEREKFYRKVLEAYFGNILVVGKDRIPIYINEY
                     TFSNSGFTKEAYNMSLDNAVENELLVNSSSLKALQTKEISITYMTGKLIGPQYSVGIP
                     IFDENGELEMAVSYSQYEYFFAGLTKLFQENNPKNSSPANLQAYGKGMQDDKIIAESD
                     QTKKMLSFLQKIASFDSTVLLVGESGTGKEVFAKYIYKNSNRSENAFIPVNCAAIPEN
                     LMESQFFGYSEGAFTGAKKSGKPGFFEMADNGTLFLDEIGDMPLSLQAKLLRVLETGE
                     ILRLGADKTIKVDVKIIAATNKDLERMVEDNLFREDLYYRLNVISVTIPPIRKRKDDI
                     EPLINYFLNEYNQRYNLSKYFSKSAIEYFKSYDWPGNVREIKNTIERMVISSHDDMLT
                     LENFTKAAERIDDKLRAFSKKNSTDDEITFSDFNMPLKDALHEFERQYILKIYNESHQ
                     NVALAAKRMGIHKTGLYKKLKHYTDPPVDKDFAE"
     misc_binding    2041..2298
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2363..3685
                     /locus_tag="P5549_09650"
     CDS             2363..3685
                     /locus_tag="P5549_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRW13375.1"
                     /translation="MKSAHREHKIYNSPMECMSRDALFALQSKRLRETVQLEYKNVPL
                     YRARMDAKGIKPEDIKTADDLRHLPFTEKTDLRDEFPFGLFAAPPSKIVRIQGSSGTT
                     GKPIVAGYTKNDVAVWTEMVARAITAAGGGKKDIIQVAYGYGLFTGGLGAHQGATKVG
                     AMVLPMSSGNTQRQILMMKELGVTMLCCTPSYATFLGETVREMGIKPEELKLKSGCFG
                     AEPWSEEMRATLEALLGIDACDIYGLTEIAGPGVAFECLEKKGMHVNEDHVMVEIIDP
                     ITEEPLPYGESGELVFSTISKTGMPMLRYRTHDICRLAAEPCICGRTHVRMDKIAGRT
                     DDMLVISGVNVFPSQIESVLVGLDGVSPHYMLVVERVKALDKLEVQVELTEAMFSDTV
                     AELERLRDDIKDKIKSVVGINAKIQLVAPKSIPRSEGKAKRIIDKRNI"
     gene            3697..4134
                     /locus_tag="P5549_09655"
     CDS             3697..4134
                     /locus_tag="P5549_09655"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HRW13376.1"
                     /translation="MFITQISVYLENARGTLREMTKILAENKIDIMALSIADTSNFGI
                     VRMIIREACIDNALNCLKSAGLMSKKNQVICIGIPNQPGALDNILSMIEAADVSIEYI
                     YSFNYNADGNALFVLRVMDSEKVISRLQENQIKIYSQADINSL"
     gene            complement(4189..4944)
                     /locus_tag="P5549_09660"
     CDS             complement(4189..4944)
                     /locus_tag="P5549_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRW13377.1"
                     /translation="MPIHYRIHCLAVGAVRTLVHTLDHEKRWLLEPAVFPILVFCLET
                     EQRRILIDTGAPNPALLSNVLRQAGILPGEIDTIILTHLHFDHCANTALFPSARFVLQ
                     QKEWESARDPVESQQDLYVPELITEVGQRDLVLVEGDCEVADSIAVHLVPGHTPGQQA
                     VSVSTVDGTYVVAGDLFGTFFNIHPAVHQLIDLTGKRIPVPVLPGEEFHPPDIRTDLP
                     DCLTSANKLLTIAGSRERIIPSHEPALSGKIYP"
     gene            complement(5047..>5376)
                     /locus_tag="P5549_09665"
     CDS             complement(5047..>5376)
                     /locus_tag="P5549_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRW13378.1"
                     /translation="KARGIKIAIGSSSKNAKLILQQIGLKDYFDAISDGTNISRSKPD
                     PEVFIKASEYLGVEPQFCLVVEDSRAGVEAAVAANMDCAAMGDAAGAGMATYDLECFS
                     DLLCYVT"
BASE COUNT         1617 a         1186 c         1206 g         1367 t
ORIGIN      
        1 agaaggcaag aaccggggct acgatcgggg tcatcatgtc aaccatttgg gttacaaccc
       61 ctttaactac tccaattttg gcaacttcca agggatggat tttatattct gtcattatca
      121 acaactcctt tttctttttt ttggagtact tggagcactc gggtactcct gcatcattta
      181 ataggacaaa catccataac cttcattaaa acgtaaaggt atccccccta aaaaaaaata
      241 tggcagtgat caacaccaaa tctattcata atatttagca aattttatgc caacctgaat
      301 ctcattatta tcgtaattca acttgctaat cccataacag ctaattttac cgataccgca
      361 atttttatat ttttgcttat gggatcattt tgaaaacttt cgtcaacaaa tatttagtag
      421 aaatttgtcc acagtagaaa catttctgct atactataga aaattttcta cttagctttt
      481 tagtacgatg aataggagtg cccatcaaat gaccatcaat attttgaaag aacctgatgt
      541 tacagaacga gagaaatttt atcgcaaagt attggaagct tatttcggca atattctggt
      601 ggtaggcaaa gacagaatac ccatttatat taatgaatac accttttcca acagtggttt
      661 taccaaagaa gcctataaca tgagcttgga taatgcggta gaaaacgaat tgctggttaa
      721 ttcatccagc ttaaaagctc tacaaacaaa agaaatttcg attacttata tgaccgggaa
      781 attaatagga ccccaatatt ctgttggcat acctattttt gatgaaaacg gtgaactaga
      841 aatggctgtg tcttacagtc agtacgaata tttcttcgcc ggtctgacaa aattattcca
      901 agagaataat cccaagaaca gtagtcccgc aaatctccag gcctacggta agggaatgca
      961 ggatgataaa atcattgctg aaagcgacca aaccaagaaa atgttatcgt ttctccaaaa
     1021 aatcgcatct tttgatagta cggtattgct ggtcggcgaa tctggaacag gcaaggaggt
     1081 ttttgccaaa tacatttata aaaacagcaa tcgcagtgaa aatgctttta tcccggttaa
     1141 ttgtgccgca ataccagaaa atcttatgga atcacaattt tttggttatt ctgaaggtgc
     1201 ctttaccggg gccaaaaaaa gtggcaaacc aggatttttc gaaatggcag ataacggcac
     1261 cctgtttctg gacgaaatcg gagatatgcc tctaagtttg caggcaaagc ttttaagggt
     1321 cttggaaacc ggtgaaattt taaggctggg agcagataaa acgatcaaag ttgatgtcaa
     1381 gataattgcc gccactaaca aggatctgga aagaatggtg gaagacaatc tctttcggga
     1441 agacttatat tacagactca atgttatctc cgtcaccatc cctccaattc gaaagagaaa
     1501 agatgatatt gagcctttaa ttaattattt tttaaatgaa tataatcaaa gatataactt
     1561 gtccaaatat ttttcgaagt ctgctattga atattttaaa agctacgatt ggccaggaaa
     1621 tgttcgggag ataaaaaata cgattgaacg aatggtcatc tcttctcatg atgatatgtt
     1681 aactctggaa aactttacta aagcagcaga acgcattgac gataaattaa gagcctttag
     1741 caaaaaaaat tcgacggatg acgagatcac cttctcagac tttaatatgc ctttaaagga
     1801 cgcactccat gaatttgaaa ggcagtatat tcttaaaata tataatgaat cccaccaaaa
     1861 tgttgcgctg gcagccaaga gaatgggaat acataagacg ggactgtaca agaaactaaa
     1921 acactataca gatccgccgg tagacaagga ttttgctgaa taatatctat tatggatatg
     1981 gttagactgt cattaaaaag ttattgacaa aaattcattg gctggaataa aatttaaaca
     2041 ctaaatgcag tgaccgggaa gtagtaagtc tgcggaagcc ttgcagagag ttgacggatg
     2101 gtgcaagcca acaaggaaca gcattccgaa ttcatcccga gagcagcatg ctgaaaaaag
     2161 attcagtagg cagagccggg ttcgcccgtt atcgcgatag ggtataagca attgccgtac
     2221 ccgatgagga tgttttcgcg agagaacatc aaactgaggt ggtaccacgg gaagtagcac
     2281 atacagcccg tcctctgtag ttcagaggac gggctttttt tgccttttcc tcgccaaaat
     2341 tttcataacg gaggtataag gaatgaagtc tgctcacaga gaacacaaaa tttacaattc
     2401 cccaatggaa tgcatgagcc gcgatgcctt gtttgcactg caaagcaaac ggttgcggga
     2461 aaccgtccaa ctcgagtata aaaacgtccc cctttaccgg gcccgcatgg acgccaaagg
     2521 gatcaagccg gaggacataa aaacggcaga cgatctaaga catttgcctt tcaccgaaaa
     2581 aaccgatttg cgcgatgaat ttccctttgg tttattcgca gcaccgccat caaaaatcgt
     2641 gcgtatccaa ggttcatcgg gaacgaccgg caaacccatt gtagccgggt atacgaaaaa
     2701 cgatgttgcg gtatggacgg agatggtagc cagagcaatt acggccgccg gaggcggaaa
     2761 aaaagatatc attcaagttg cctatgggta tggtttgttc actggcggtc tgggggccca
     2821 tcaaggcgca accaaggttg gcgccatggt gttacccatg tccagcggca acacccagcg
     2881 ccagatcctg atgatgaaag aactgggggt aaccatgctc tgctgtaccc cttcctatgc
     2941 aaccttcctg ggagagaccg tccgggagat gggtatcaaa ccagaagaat taaaattgaa
     3001 atccggctgc tttggcgctg aaccttggtc ggaagagatg cgagctaccc tggaagcgct
     3061 tttagggata gatgcctgtg atatttatgg tttaaccgaa atcgccggtc cgggagtcgc
     3121 ctttgaatgt ttggaaaaga aaggcatgca tgtcaatgaa gatcatgtta tggtagagat
     3181 aatcgatccg ataaccgaag aacctctacc ctatggggaa tcaggcgaac tggtcttttc
     3241 caccatatcc aaaaccggta tgccgatgct tcgttatcgg acccatgata tctgccgatt
     3301 ggcggctgaa ccctgtattt gtggacgcac ccatgtgcgg atggataaga ttgccggacg
     3361 taccgatgat atgctggtaa tcagcggcgt caatgtgttt cccagtcaga tcgagtccgt
     3421 attggtcggc ctggatgggg tctctcccca ttacatgctg gtggttgaac gggtcaaagc
     3481 cttggataaa ctggaagtac aggtggaact gaccgaagca atgttctccg ataccgtcgc
     3541 tgaactcgaa agattaagag acgacatcaa ggacaaaatt aaatccgtgg tgggaatcaa
     3601 tgccaaaatt caattggttg caccgaagtc cattccccgc tcagaaggaa aagccaaaag
     3661 aattattgac aaaagaaata tttaggaggg caggctatgt ttattacgca aatctccgtg
     3721 tatctggaga acgcccgggg aaccttacga gaaatgacca aaatattggc cgaaaacaag
     3781 attgatatta tggccctttc gatcgccgat acttcaaatt ttggcattgt tcgcatgata
     3841 atccgggaag cttgtatcga taacgcattg aattgtctga aaagcgccgg ccttatgtcc
     3901 aaaaaaaacc aagtaatctg tatcggcatt cccaatcaac cgggtgcatt ggacaatatc
     3961 ctgtccatga tcgaagcggc cgatgtctcc atcgagtaca tttattcttt taactacaat
     4021 gcagatggaa acgccttgtt tgtgttacgc gtcatggaca gcgaaaaagt catctcccgc
     4081 ctgcaggaaa accagataaa aatatactcg caggcggaca tcaacagctt gtaaatgcct
     4141 ctgaagggag catacgtgct ctgcttgatt aatcgggccg gcacaggcct acggataaat
     4201 tttgccggat aaagcgggtt catggctggg aatgattcgt tcccggctgc ccgcaattgt
     4261 taacagcttg ttggcactgg tcaggcaatc aggcagatcc gtacgaatgt ccggcggatg
     4321 gaactcctct cccggcaaga caggaacggg gatccgcttg ccggtgagat caatcagttg
     4381 atggacagcc ggatggatat taaagaaggt cccaaacaaa tctccggcca cgacatatgt
     4441 accgtcaacc gtagacacag aaaccgcctg ttgtcccggg gtatgaccgg gaaccagatg
     4501 cactgcaatg ctgtcggcga cctcgcaatc accttccacc aataccaaat cacgctgacc
     4561 gacctcagta atcagctccg gcacataaag gtcctgctgc gattcgaccg ggtcgcgtgc
     4621 actctcccat tccttttgct gaagaacaaa gcgggcggag ggaaacaaag ccgtgttggc
     4681 acaatgatca aaatgaagat gggtcaggat gatcgtatct atttcaccgg gcagaatacc
     4741 tgcctgtctc aggacgttgc tcagcaaggc cggattgggt gccccggtgt caatcagaat
     4801 acgtctctgc tctgtttcca aacaaaaaac gagaatggga aataccgccg gttccagtaa
     4861 ccagcgcttc tcgtgatcca acgtatgaac caatgtccgc acggccccga ccgccagaca
     4921 atgaatgcga taatggattg gcaaattcaa ttcacttcct taatttcttt gcttgattta
     4981 tttattcccc ttatttcact gcgaaatatc tattctgcgc cgcccggtgg gcattgaaga
     5041 aattgatcat gtaacatagc acagcagatc tgaaaaacac tccagatcat aggtggccat
     5101 tccagccccg gcggcatctc ccatggccgc acaatccatg ttggcagcca cagcagcttc
     5161 caccccggct ctggaatctt caaccaccag acaaaactgt ggttcgacgc ccaaatactc
     5221 actcgctttt ataaacactt ccgggtccgg tttggagcgg ctaatgttgg taccgtcgga
     5281 aattgcgtca aagtaatctt tcagtccgat ctgctgcaaa attaacttgg catttttact
     5341 ggaagatccg atggcgattt taattccccg ggcttt
//
LOCUS       DAOTHZ010000110         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_18546_length_5280_cov_6.688804, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000110 DAOTHZ010000000
VERSION     DAOTHZ010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18546_length_5280_cov_6.688804"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1533)
                     /locus_tag="P5549_09670"
     CDS             complement(106..1533)
                     /locus_tag="P5549_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRW13379.1"
                     /translation="MKLAPKWQSLIIISIGIFMATLDGSILNIANPTIAEALQTDMQQ
                     VQWIVTVYMLVITASLIFFGRLGDRLGNERVYATGFLIFALGSFLCSLSASLPFLIAC
                     RVVQAMGASMMMATGMGIISNTFPDGERGKALGLTGMIVGLGNMTGPGLGGFLVTQFT
                     WPVLFLINVPIGLLGFWLGMRFLPKQQFNPNLSGHDWLGTIVFALLTGLLIYSVAHAS
                     AWIGLVLLLIPLFCWMENRSVHPLLDFTLFKIKNFSYGMVMASGIYITQMTVLFLLPF
                     FLEEVFHLNPSRSGLLITVTPICMAITAPLAGTLSDKVGSRKILSLSLFIVTGAFLVL
                     TTLKAQFEPGRTIAGLALLGIGMGMFGSPNNSSIFSSVPKEKAGYVGGFIATVRNLSF
                     ALGTAGSVSVFYFVLALGQKSMSYNQAYIHASNTVYLLAAAITGAGFIFSLLGRWGGN
                     PADDQTQVDCENSCKLSCKNSFPNA"
     gene            complement(1606..2568)
                     /locus_tag="P5549_09675"
     CDS             complement(1606..2568)
                     /locus_tag="P5549_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRW13380.1"
                     /translation="MDTINKRYIWIFFIILLVVMAAAYIINTLLPENPKPYLKTSTGE
                     TTSGNFYTVRDEKGMTIFQTGLAVHIDDRYISENDVEYVVIKIDGLNAQASIVQTQDK
                     PNLELNDHITSSTLRLNQAVPTVVAKPTHVVMYHTHDDESYIPTDGKASQPAQGGVLK
                     VGEALTKTLKNAGVSVTHNQDSHGPHDVNAYHRSRRTAVQLIKEQPDAILDIHRDSAP
                     VKAYYTTINGIDCARVMIVIGRSNPNMKTNLAYARQIKASADKLYPGLCRGIFMGRGD
                     YNQDLYPTAILFEIGTEKISRNLAENSARCLGDVLIRELGARRS"
     gene            complement(2696..3970)
                     /locus_tag="P5549_09680"
     CDS             complement(2696..3970)
                     /locus_tag="P5549_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRW13381.1"
                     /translation="MNTEKKKSVRPSIIKDSRLAPLGHNKLEWVKNNMPVLNILKQEL
                     EQSKPLQGKTILCCLHLEAKTGYLLQTLMAAGADVVACASNPLSTQDDVVAALVDSGI
                     TVYAIHGESSEEYHQYLEKALDTMPDAVIDDGADVISMLHKSRRGQAAAIIGGCEETT
                     TGIVRLRSMDHDNTLLFPMIAVNDAYMKYLFDNRYGTGQSVWDGINRTTNLVVAGKYV
                     VVVGYGWCGKGVALRAKGMGAKVIVTEIDPIKANEAIMDGFRVMNMQEAACLGDIYIT
                     VTGNINVIRKEHMQVMKDNAIMCNAGHFDVEISKPDLDALAVSKKTIKPNIEEYLLED
                     GRRLYLLAEGRLVNLAAGDGHPAEVMDLSFSLQALSLLYVIENRANLDNHVYNVPKEI
                     DQRVACLKLQAEGIKIDVLTEEQKSYLDSWEN"
     gene            4115..>5280
                     /locus_tag="P5549_09685"
     CDS             4115..>5280
                     /locus_tag="P5549_09685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRW13382.1"
                     /translation="MKVLMLSWEYPPHMVGGLGQHVYDISRYLVRQGMEVHIITPRVK
                     NYPDQDTFAGVFIHRVGNPISEASPDDFKGWTFTFNSEAIREAVRLNSEAGIDLIHAH
                     DWMVAYAGRSISKIFGIPLVTTIHATEYGRNIGLHNRTQREINEIEKNLALEAHQIIC
                     CSQYMQDEISSLFGVHRSGIKIIPNGVEPEQFKELPEHPPVEFDPQEKALVFLGRLVP
                     EKGVWQLLNCFPRVLESVPEAKLYIGGRGPQKRILEQRSQKLGIAEQVIFTGFLKEKD
                     RNYVYHHAKAAVFPSLYEPFGIVALEAMATNTPVIVSEVGGLSEIVEHRQTGLKVPPG
                     HESRLAEAIIELLTNTEYARQLAENAAQAIASTYSWETIAHSTIAVYEEVIARM"
BASE COUNT         1383 a         1258 c         1291 g         1348 t
ORIGIN      
        1 aacctccgca cccggctccg gaggccggtg ctctatccac tgagctacgg gcgcacatgc
       61 attaagtatt atagcagaac aaaaatcata ggtcaaagaa atcaatcatg cattgggaaa
      121 agaatttttg caagataatt tgcaagaatt ctcgcaatca acctgggttt ggtcatccgc
      181 cggattgcct ccccagcgtc ccagcaggga aaagatgaat ccggcaccgg taatggcagc
      241 ggccaacaag taaacggtat tgctggcgtg tatgtaagcc tgattgtaag acatgctttt
      301 ttggccgagt gccagcacaa agtaaaagac gctgacggat ccagcagttc cgagtgcaaa
      361 ggaaagattg cgcacggttg caataaagcc gcctacgtag ccggctttct ccttgggtac
      421 ggaggaaaag atggaactat tattggggga gccaaacatc cccataccga ttcccagcaa
      481 ggccagtccg gcaattgttc tgccgggttc aaattgtgcc tttagggttg tcaaaacaag
      541 gaaagcaccg gttacaatga atagagacaa agataggatt ttgcgggaac caactttgtc
      601 agacagcgtt cctgccagag gcgcagtaat ggccatgcaa atgggagtca cggttatcaa
      661 caaccccgac ctggagggat tgaggtgaaa tacttcctcc aagaaaaagg gcaggaggaa
      721 caacaccgtc atctgggtaa tgtagattcc ggaggccatt accattccat aggaaaagtt
      781 cttaatttta aataatgtaa aatcaagaag gggatgtacg gatctgtttt ccatccagca
      841 aaaaagcggt atcagcaaca ataccaaccc aatccatgcg ctggcatgag ctacgctata
      901 gatcagcagt ccggtcaaca aggcgaatac tattgtgcct aaccagtcgt gaccgctgag
      961 gttcggattg aattgctgtt ttggcaaaaa gcgcattccc agccaaaagc caagcaaccc
     1021 gataggcaca ttgattaaga acagaaccgg ccaggtaaat tgggtcacga gaaagccgcc
     1081 caacccggga ccggtcatgt tgcccagtcc aacgatcatt ccggtcaacc ccagagcctt
     1141 gccgcgctct ccgtcgggaa aggtgttgga aatgatgccc atacctgttg ccatcatcat
     1201 actggcgccc atggcttgaa caacccggca ggcaatcaaa aagggcaggg aagcggagag
     1261 actgcataga aaggagccca gcgcaaaaat caggaatcct gttgcataga ctctctcatt
     1321 gccgagacgg tcacctagac ggccaaagaa aatcagactg gcggtgatta ccagcatata
     1381 cacggtaacg atccactgca cttgctgcat gtctgtttgc agggcctcag caatggtggg
     1441 attggctata ttaaggatgc tgccatccag cgtggccatg aatatgccga ttgaaatgat
     1501 aattaatgac tgccatttgg gcgccagttt catgttatcc cttccccatc cgcattgttc
     1561 agctattcta ctatattata cgtgataggg aaaaagacag gacgattaac ttctacgcgc
     1621 acccaattct ctgatcagca catctcccag acagcgggca ctattttcag ccaaattacg
     1681 agaaattttt tctgttccga tttcaaacag aatagcggtg ggatagagat cctggttgta
     1741 atcaccgcgt cccatgaaga tgccccgaca caatcctgga tataatttgt cagcgcttgc
     1801 cttgatctgc cgggcataag ccaggttggt tttcatgttg gggttggagc ggccgatgac
     1861 tatcatcacc cgggcacagt cgatcccgtt gatggtcgtg taataggctt tgaccggtgc
     1921 agaatcgcga tgaatgtcca ggattgcatc cggctgttct ttgatcaatt ggacagcggt
     1981 acggcgggaa cgatgatagg cattgacatc atgcggacca tgactgtcct ggttgtgcgt
     2041 tacactgacg cctgcatttt taagtgtttt ggtgagagct tcgcctactt tgagaacccc
     2101 accttgggct ggctggctgg ccttgccgtc cgtcggtata taggattcat catcgtgggt
     2161 gtgatacatg acgacatgag tcggtttggc aacgacagtg ggaacagctt ggtttaggcg
     2221 caaggtactt gaagtgatgt ggtcattaag ctccaggttt ggcttgtcct gtgtctgcac
     2281 aatggaagct tgggcattca agccgtcaat tttgataact acgtattcca catcgttttc
     2341 gctgatatag cgatcgtcaa tatgcactgc cagtcctgtc tgaaagatgg tcatgccttt
     2401 ttcatcccgt accgtgtaaa aattgccgct ggtcgtttca ccggtactgg tttttaaata
     2461 gggttttgga ttttcgggca gcagagtatt gattatgtaa gctgctgcca tgactaccag
     2521 caggatgata aagaatatcc atatatatcg cttgttgatc gtatccatcc ttttccgcct
     2581 ttcttttctc ctgtgcttat actattcttt caaaaaggcg aaacaaatat tcagccaggc
     2641 aggaaagata ctccccagcc tggctgttct ttacgtatat attgaatggt atgatttagt
     2701 tttcccagct gtccaggtac gatttctgtt cctcggtaag tacgtcaatc tttatccctt
     2761 cagcctgcag ctttaggcac gccacccgtt gatcgatttc tttcggaacg ttgtagacat
     2821 gattgtcaag gttggcacgg ttttcgatga catagagcag ggacaaggct tgcagcgaga
     2881 agctcaaatc catcacttcg gcgggatgtc cgtctccggc ggccagattg acgagtcgtc
     2941 cttcagccag cagataaagc ctgcgtccat cttccagcag atattcttcg atgttgggtt
     3001 tgatggtttt cttgctgacc gccagggcat ccagatccgg tttggaaatc tccacatcga
     3061 aatgaccggc attgcacata atggcattgt ctttcataac ttgcatatgt tccttgcgaa
     3121 tgacgttgat gttgccggtc acggtgatgt aaatatcacc cagacaagct gcctcttgca
     3181 tattcatcac acggaagccg tccataatgg cttcgttggc tttaatggga tcgatttcgg
     3241 taacaatcac tttggcgccc attcctttgg cacgcagagc cacgcctttg ccgcaccatc
     3301 cgtaacctac caccacaaca tatttgccgg ccaccaccag attggtggta cgattgatgc
     3361 cgtcccagac tgattggccg gtgccgtagc gattatcaaa caaatatttc atataggcgt
     3421 cattgactgc aatcatggga aacaacaagg tattgtcatg gtccatggat cgcagccgta
     3481 cgatcccggt ggtggtttct tcacagccgc cgataatggc agcggcttgc ccgcggcggc
     3541 ttttatggag catactgata acatcggcgc cgtcatcaat gacggcatcg ggcatggtgt
     3601 caagggcttt ttccaaatat tgatgatact cttcactgct ctccccgtga atcgcataga
     3661 cagtgatgcc tgaatccacc aatgctgcca cgacatcatc ctgcgtggaa agcggattgg
     3721 aggcacaggc tacaacatcc gcaccggctg ccatgagcgt ctgtaaaaga taaccggttt
     3781 tggcttccag gtgcagacaa cacagaatgg ttttcccctg caaaggtttg ctctgttcaa
     3841 gttcctgttt tagaatatta agaacgggca tattattctt cacccattcc aatttgttat
     3901 gccccagcgg agccaggcgt gaatctttta taatggatgg tctgacggat ttcttttttt
     3961 ctgtattcat ttatgttacc tcctggaatg atttaaatcc ttctttgaaa gtataacatt
     4021 acaacatcaa aagcaaaata ttttacttaa gtaacaatta tctggtatta ttaggtatta
     4081 gatataatca atccgggata agaaggggtt tggcatgaag gtattaatgc tcagctggga
     4141 atatcctccc cacatggtcg gaggactggg tcagcatgta tatgatatca gccgttatct
     4201 ggtcagacaa ggtatggaag tccatattat tactccacgg gttaaaaact atccggatca
     4261 agataccttt gccggtgtct ttattcaccg ggtcggcaat cccatcagcg aagcctcccc
     4321 ggacgatttc aaaggatgga cgtttacttt caacagtgaa gcaattcgag aggccgtccg
     4381 ccttaattct gaagccggca ttgatctgat ccacgcccat gactggatgg ttgcctacgc
     4441 cgggcgatcc atctcaaaaa tcttcgggat tccgttggtt accaccattc acgcaaccga
     4501 atatggaagg aatattggtc tgcacaaccg tacccagaga gaaatcaacg agatcgaaaa
     4561 aaatctggct ttggaagctc atcaaatcat ctgctgcagt caatacatgc aggatgaaat
     4621 ctcctccctc tttggtgtgc atcgcagcgg gattaaaatc attcccaacg gcgttgaacc
     4681 cgaacaattc aaggaactgc ccgaacatcc tccggtggag tttgatccac aggaaaaggc
     4741 gttggtcttt ctgggaaggc tggttccgga aaaaggtgtt tggcagctgt taaactgttt
     4801 tccaagggtt ctggagagtg ttccggaagc caaactatat atcggcggga ggggaccgca
     4861 aaagcgaatt ctcgaacaaa gaagtcagaa gctgggaatc gcggaacagg tgatattcac
     4921 cggttttctg aaagagaaag accgaaatta tgtgtatcat catgccaagg cagcggtatt
     4981 tccaagtctg tatgaaccct tcgggattgt tgccctggaa gccatggcca ccaatacccc
     5041 ggtcattgtc agcgaagttg ggggattatc ggaaatcgtc gaacatcggc aaacaggttt
     5101 gaaggttccg cccgggcatg aatccagact ggcggaagcc atcatcgaat tattgacgaa
     5161 tactgaatac gcccggcaat tggctgaaaa cgctgcacaa gcgattgcct caacttatag
     5221 ctgggagacg atagcccaca gtaccattgc ggtatacgaa gaagtcatcg cccgtatgca
//
LOCUS       DAOTHZ010000111         5071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_19553_length_5071_cov_5.137161, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000111 DAOTHZ010000000
VERSION     DAOTHZ010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19553_length_5071_cov_5.137161"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..655)
                     /locus_tag="P5549_09690"
     CDS             complement(32..655)
                     /locus_tag="P5549_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13383.1"
                     /translation="MSIHRRHFTRKGFVLITILLIMALFWPVNALAAPERIILPDEDS
                     SGETMGETGLVLATKDGGQLRVNAVLAGSGGGTDISLTKMSKDGRISWSKSYGGSGDE
                     QARSIQETSDNGFIITGYTSSQGTGGRDLYMLRLSPLGEKKWETAAGDSLDDEGIMVQ
                     QTSNGDFVAVGYSQRQRTVSGSQTQTQDADIMAVRYDRNGKKLWQRY"
     gene            complement(717..2090)
                     /locus_tag="P5549_09695"
     CDS             complement(717..2090)
                     /locus_tag="P5549_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13384.1"
                     /translation="MKISRREKNLLLVAVYLLIIFGGYKLMIAPNQPKLAEVQAVNQQ
                     LNQQIEQAVESGKKPDDSQSAISKELAAYMKLEEQLPRDKQLVELVDQIGTIASDNKV
                     ILLGVNYTQAKEAPKTAEEAAQQDQDNVKAALAGVSKMDLTLNVNGSYYNLLGFLQDL
                     ERSSRIIIVNSAGMSVGQFEDKTTTGSTGGTGTTYTSNNPPPPAPEPKITRIQPNVST
                     GSAAAPVQVTVNEPAIPEIYKYNMSNVQLNLQITSFYDSSPSEIEKLLGTNLFHQLTG
                     QGSALQTDENLAKVTAAVKKYWNQDDPTADWSNPRNPGEDCWNGQLLKYLEGKYNNTN
                     MGKNVRDLVNPLAATVNPESKWAGTAILHWDKILDLNMTSTVKPVDPDTKQTFTNVKT
                     RDFLPAAVYITYLPNYAPDKVETLSQSQKQAILGSVIVWQAQNSKTAPVVYRIDENGK
                     MQDLQKL"
     gene            complement(2087..2650)
                     /locus_tag="P5549_09700"
     CDS             complement(2087..2650)
                     /locus_tag="P5549_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRW13385.1"
                     /translation="MSGQPEVQINLINYKLEQKSTRQTMMKEWGIYLLIALLLVGGLF
                     VYSNSMEKRIAALEKENASLQAELKQTTVQASTTQTGKKIEAAMKTRSLVVHSLQKMQ
                     NEYVPVFEELGDLNGSGILFTSIDVQNNTVNTRGYASSHGNLVALLQALRDSEQFSDP
                     ANLQLSTDEDTREISFSMQMGLEVEKE"
     gene            complement(2935..3660)
                     /locus_tag="P5549_09705"
     CDS             complement(2935..3660)
                     /locus_tag="P5549_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRW13386.1"
                     /translation="MLFILGLFIGSFLNVLIYRIPRGESVVWPGSHCAQCGHGLKPLD
                     LVPVVSYLLLRGRCRYCGTKISLRYPLVELLTAFSFLLVYLQYGYSVQTAAGCVLVAM
                     LVVVTFTDIDEGIIPDVITYPGMLIGLAFSFFTIGIKSALFGMLGFGLVFFLIALLSR
                     GGMGGGDVKLVAVIGAFVGPLHSVLVFILSSLAGGLWAIGLLLFRGAGRKTEVRFGPF
                     LALAALLVFLYGQQLLDFYLGLF"
     gene            complement(3859..4698)
                     /locus_tag="P5549_09710"
     CDS             complement(3859..4698)
                     /locus_tag="P5549_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRW13387.1"
                     /translation="MGRNPRIEYEGAIYHVIQRGTNREYIFGEHRWKKNLLQEIIKSI
                     EVDEMDLFAFVIMGNHYHLALRTEQTPLSKIMHRINSNFGRYYNRDRSRTGSVFEGRY
                     KAFPIENENYLLSVIRYIHRNPVRASICSSVEEYSWSSDQAYRGRGFRFVRTDVLFSI
                     LSSNPNRAKLEYNRLMRLKDDYDESAIKYLGSEEFWLQKTYKPEAKAGKKSLETILLE
                     SGADANSIGLIKNGSKQRDLMEYKRDYIQRAFAAGYSMEEIGKHISISGVAVGKILRK
                     LES"
BASE COUNT         1240 a         1345 c         1134 g         1352 t
ORIGIN      
        1 gcttaactga gctaaggcat tttctaactc tctagtatct ctgccagagt ttcttgccgt
       61 ttctgtcata acgaaccgcc atgatatcgg catcctgggt ctgcgtttga ctgccgctta
      121 cggtccgctg ccgctgggaa tagccgaccg ccacaaaatc tccgttcgag gtctgctgta
      181 ccatgatgcc ttcatcatcc aggctgtcgc cggcagcggt ttcccacttt ttctctccca
      241 acgggctcaa gcgcagcata taaaggtcgc gcccgccggt accctgggaa ctggtatagc
      301 cggtgattat aaatccattg tcagaggtct cctgaatgga gcgggcttgc tcgtctccgc
      361 tgccgccata gcttttgctc cagctgattc tgccatcctt ggacattttg gtcagagaga
      421 tatcagtgcc cccgccgctg ccggccagca cggcattgac ccgaagctgc ccgccatcct
      481 tggtagccag caccagtccg gtctccccca tggtttcccc gctgctgtcc tcatccggca
      541 agataatccg ttcgggcgca gccaaggcat tgaccggcca gaacaatgcc atgatcagca
      601 aaatcgtgat caagacaaaa cctttacggg taaaatgacg tctatgtatg ctcatgtttg
      661 ctcactcctt taccggcagc aggctcgctg cctgcccgtc gttggttgac gctatcctac
      721 agcttctgca gatcctgcat cttgccgttt tcatcgattc tatataccac cggggcggtt
      781 ttgctgttct gggcctgcca tacaatcaca ctgcccagga tggcttgttt ttggctttga
      841 ctgagcgttt ccaccttgtc cggcgcataa ttgggcaaat aggtgatata taccgcggcc
      901 ggcaggaaat cacgggtttt gacattggta aaggtttgtt tcgtatccgg gtcaaccggt
      961 ttgacggtac tggtcatgtt caggtcgagt atcttgtccc agtgcaggat ggccgttccc
     1021 gcccatttcg attcaggatt gacggtcgct gccagaggat tcaccagatc ccgcacattt
     1081 tttcccatat tggtattatt atattttccc tccagatatt tcagcaactg cccgttccag
     1141 caatcttccc ccggattacg cgggttgctc caatcagctg tcggatcgtc ctggttccag
     1201 tattttttaa ccgctgccgt gaccttggca agattttcat ctgtctgcaa agccgatccc
     1261 tgtccggtca gctgatggaa cagattcgtt cccagcagct tttcaatctc cgagggagag
     1321 gaatcataaa agctggtaat ctgcaggttc agctgtacat tgctcatatt gtatttgtat
     1381 atttccggta tggcgggctc gtttaccgtg acctgcaccg gggcagccgc tgaaccggtg
     1441 gagacattgg gctgaatccg cgtaatttta ggctctggtg ccggcggagg cggattgttg
     1501 ctggtatagg tcgtgccggt gccgcctgtg gaacccgtcg tggttttatc ctcaaactgc
     1561 ccgaccgaca ttcccgccga attgacaatg ataatacggg aagacctttc caaatcttgc
     1621 aggaaaccca gcaaattgta gtaagacccg ttgacattga gggtcagatc catcttggaa
     1681 accccggcca gcgccgcctt tacattgtcc tgatcctgct gggccgcttc ttctgccgtt
     1741 ttgggggctt ctttggcctg ggtatagttg acccccagca agatgacctt gttgtcgctg
     1801 gcgattgtgc cgatctggtc gaccagttcc accagttgtt tgtcacgcgg cagctgctct
     1861 tccagtttca tataggctgc cagttccttg ctgatggcgg actgactgtc atccggtttt
     1921 ttaccgctct ccaccgcctg ctcgatctgt tggttgagtt gctggttgac cgcttgcact
     1981 tcggccagct tcggctggtt gggagcaatc atgagtttgt agccgccgaa aataatcagc
     2041 agatagacgg caacgagaag cagatttttt tcccgtctgg aaattttcat tctttctcca
     2101 cctccagacc catctgcata ctaaacgata tctcccgggt atcctcatcg gtagataact
     2161 gtaaattggc cggatcacta aattgttccg agtcccgcag ggcctgcagc aaagccacca
     2221 aattgccgtg actggaggca tatccccggg tgttcacggt attgttctga acatcaatgc
     2281 tggtgaataa tatgccggag ccatttaaat cgcccagttc ttcaaacacc ggcacgtatt
     2341 cattctgcat tttctgcagg gaatgaacca ccaggctgcg ggttttcatg gctgcctcaa
     2401 tttttttacc ggtttgcgtg gtggaggcct gtacggtcgt ttgctttaac tcggcctgca
     2461 ggctggcgtt ttctttttcc agggccgcaa tgcgcttttc catagagttg ctgtagacaa
     2521 acaatccgcc gaccagcaac aacgcgatca gcagatagat gccccactcc ttcatcatgg
     2581 tctgacgggt gcttttttgt tccagtttat aattgattag attaatttga acttcaggct
     2641 gtccgctcat agaaacctcc gcaccaacct tatttgtttc tttcatttta ctatgccttt
     2701 atagccatta taattcaata ggaaataaat gaattccttt tattttcgca gatatgagaa
     2761 atatttttat taatttgcaa aatcctgtat ttctttttgt tattaataag aaaattcgtt
     2821 ttggttatta tgaaaatctg tttctgtcat cctgaacgaa gtgaagcgac gatgccatat
     2881 ggcatctagt accgaaagcg ataagaaggc aaacgaagcg aagtctgagc gtccctaaaa
     2941 gagtcccaga tagaagtcga gcagttgctg cccgtagagg aagaccagca acgccgccaa
     3001 cgccaggaag ggcccaaaac gcacctcggt cttgcgcccg gcgccgcgaa acagcagcaa
     3061 gccaatcgcc catagcccgc cagccaaaga ggaaagaata aagaccaaca cactgtgcaa
     3121 aggcccgacg aatgcgccga taaccgccac cagttttaca tcgccgccgc ccatgccgcc
     3181 ccgcgacaaa agcgcgatca ggaaaaacac cagaccaaag cccagcatgc caaagagcgc
     3241 acttttaatc ccaatggtga aaaaagaaaa agccagcccg atcagcatcc ccggataggt
     3301 aatgacatcc ggaatgatgc cttcatcaat gtccgtaaag gtcaccacaa ccagcatcgc
     3361 caccagcaca cacccggcag ccgtctgaac ggaatagcca tattgcagat aaaccagcag
     3421 aaaggaaaac gcggtcagca attcgaccag cggatagcgc agcgagatct tcgtgccgca
     3481 gtaacggcac cgcccccgca gaagaagata actcaccacc ggcacgagat ccagcggctt
     3541 cagaccatga ccgcactgcg cacagtgact tcccggccaa accaccgact ccccccgcgg
     3601 gatgcgatag ataagcacat tcagaaaaga cccgatgaat aagccaagaa taaataacac
     3661 gtgccaggca cctaactttc taactttcgt aatattttcc cgacggcaac acccgaaatg
     3721 gagatgtgcc tgccgatctc ttcaggaata ctcttcaacc gaggagcaaa tcgcagcgcg
     3781 aaccgggttg cggtaaatat atcttatcac acttggaaga tagttttcat tctcaatcga
     3841 gtgccacgtg ccaggcacct aactttctaa ctttcttaat attttcccga cagcaacacc
     3901 cgagatagag atgtgcttgc cgatctcctc catcgaatag ccggcagcaa aagccctttg
     3961 gatataatca cgtttatatt ccattaaatc tctttgtttg gagccgtttt tgatgagccc
     4021 tatgctgttg gcatctgccc ctgattctag cagaatcgtc tccagactct tctttcccgc
     4081 ttttgcttcc ggtttatagg ttttttgtaa ccagaactct tctgaaccca ggtacttgat
     4141 agcactttca tcataatcgt ctttgagtcg cattaaacga ttgtactcca gtttggctcg
     4201 attaggattg gaactgagga tggaaaacaa aacatcggta cggacaaaac gaaagcctct
     4261 ccctcgataa gcctgatcac tgctccagga atactcttca accgaggagc aaatagaagc
     4321 acgaactggg ttgcgatgta tatatcttat aacacttaga agatagtttt cattctcaat
     4381 cggaaatgct ttatatctgc cttcaaaaac actgcctgtc cggctgcgat cccgattata
     4441 atatcgacca aagttggaat taatgcggtg catgatttta ctaagcggcg tttgctcagt
     4501 acgtagcgct aaatgataat gattgcccat gatcacaaat gcaaacaaat ccatctcatc
     4561 cacttctatg gatttaataa tttcttgaag cagatttttc ttccagcgat gttcaccaaa
     4621 gatgtattct cgattggtgc cgcgttgaat aacatgataa atggctccct cgtattcaat
     4681 cctcggattc ctgcccacaa aaatcacccc caactaacga cttcagttta tcaggcaaaa
     4741 gattcttcta caaccattta gttctatttt tgattataca ttaacaataa aaaagccgat
     4801 gaaaaacctc ctgctacctt ttcagtaaca ggaggctcgc ttgttttatt tacttaccag
     4861 gaaagttaga aagttaggtg cctggcacgc ttggtcccca atacttataa ttaagagcat
     4921 aataaaagcc tcctgctacc tttttcagta acaggaggct cgcttgtttt attaacttac
     4981 caggaaagtt agaaagttag gtgcctggca cgcttggtca cgcttgtttt atttacttac
     5041 caggaaagtt agaaagttag gtgcctggca c
//
LOCUS       DAOTHZ010000112         5008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_19898_length_5008_cov_5.806784, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000112 DAOTHZ010000000
VERSION     DAOTHZ010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19898_length_5008_cov_5.806784"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="nrfD"
                     /locus_tag="P5549_09715"
     CDS             <1..534
                     /gene="nrfD"
                     /locus_tag="P5549_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRW13388.1"
                     /translation="VWSNPLIAFLFFVSAISTGICMIILFGKYWDTLENLFRRIIPAR
                     LKPEKWMPEQISLTFDHKTIRMLIGIDAVIIIIELMVISTLIISYMLRPTGDTALGAI
                     ITSGYAFGFWTGVVLIGLAIPLLLGILELRDRLTVKMNRIVSIVEPLMVLTGGFILRW
                     VVVYGGQTVYPILTYYR"
     gene            complement(625..1566)
                     /locus_tag="P5549_09720"
     CDS             complement(625..1566)
                     /locus_tag="P5549_09720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRW13389.1"
                     /translation="MFFNQLEIIPVIAETKSFSKAARLLHLSQPAISSKVQAMEDYYK
                     VKFFNRTAQGVTLTEAGKVVLAYADRLISLHQSMDTDLQQINDLYCSSLSIGASCTAG
                     NFAMPCSIQAFKAKYPASRIKLDIDNTNATLKKLLNKEIDVAVVEGQINFPGVVSQFL
                     DRRELVLVFPNTDAFKCRKEIRLRDLKEKPFVMREKGAAIPAILKSTFSDLGYDLYEF
                     NIVSEMNSIHSVKAAVEGGMGVSIVPRIAVTKELNAGALKEVKIKEIEELFLDVNLVY
                     RADEEPSHITRRFIRFLTHANNSGLCWNGEKKELLNA"
     gene            complement(1594..2118)
                     /locus_tag="P5549_09725"
     CDS             complement(1594..2118)
                     /locus_tag="P5549_09725"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRW13390.1"
                     /translation="MNLLYGLIAGILFGFIMQRARVIRYDKQIGALLLQDMTIVKFMF
                     TAILVGMAGIYLLGDVGYLKISGRPLIIGATLIGGGLFGIGWALLGYCPGTSWGALGE
                     GRLDALVGILGLFAGAFIYAEIYPFLAETVLTWGNMGNLTIPDALGINHWFVIAVMVA
                     GGILMFRWLEKKGL"
     gene            complement(2121..2639)
                     /locus_tag="P5549_09730"
     CDS             complement(2121..2639)
                     /locus_tag="P5549_09730"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRW13391.1"
                     /translation="MNGDHKNGLNPYIGGALTGLLIILSVAVSGNYFGTSTSIVQLLG
                     MLEKFISPFQVAQTEYFKVVNPVISWQILFVTGIFIGGFLGATLFGEFKWRFFPDLWK
                     NHLGTSYVKRGLFAFLGGMIAMIGARLAGGCPSGQMSAMILLSLSALVAMIMFFAAGI
                     ITVKLVYKGGGK"
     gene            2864..3451
                     /locus_tag="P5549_09735"
     CDS             2864..3451
                     /locus_tag="P5549_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13392.1"
                     /translation="MKSRWTKLWVLGMALLLVGGMAFSAVAADSNNTTTGLLQQGIKA
                     GQQRAQTALKAVVELTGLSADEVRAQRAAGSSLAGIAVDKGISEQTVIDKVAAERTAV
                     LDQLKADNKITADQYNACVTNMQAKIKANIERTGIGPANGGQGQGGAYMRGAGQGQGA
                     GAGQGNGRGMGQGQGMGRGAGANQANCIYNVPAAN"
     gene            3595..4278
                     /locus_tag="P5549_09740"
     CDS             3595..4278
                     /locus_tag="P5549_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRW13393.1"
                     /translation="METVILLVEDEKKLRDMLIAHLQQEGFVVVPAADGMEALHIWQE
                     IKPDLLVLDLMLPRLSGWEVLKIIRQKDNTPVLVLTARAEEVDKLLGLELGADDYMTK
                     PFSPRELSARIKAILRRSRPTVQNTQTVFRNLRIDSERMEAYVGDMLLPLTVTEFKIL
                     LLLTDNPGQVFSRLQILERVFGDMYEGYERTIDTHISNLRHKIEAAAISQVSIKTVYG
                     VGYKMTAGE"
     gene            4281..>5008
                     /locus_tag="P5549_09745"
     CDS             4281..>5008
                     /locus_tag="P5549_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRW13394.1"
                     /translation="MAKAGLAKKITTTLITVAIISVLITAIPMLLMTRQQFSNYIGKY
                     DQAMLQQWQPIVAEYYAQNGSNGLQDLLESNPMGGSSMGNGRGMRRGEAGMRSKQGQR
                     LLVTDMKGIVLADSKGVLLGQQAEFDPADVSSAPLLAEYQQIGTLYVISPLGSGLASL
                     ENDFITRLTGSTVVLAIFMAMMALILGLLLGKRVSEPLGELSIAIHNVARGKLDQRLD
                     LHGDEEFEQLGHDFNLMAQNLEHA"
BASE COUNT         1372 a         1190 c         1248 g         1198 t
ORIGIN      
        1 gtctggagta atccgctgat agctttcttg ttctttgtgt cggccatttc caccggaatt
       61 tgtatgatca ttctcttcgg caagtattgg gatacgctgg agaatttatt ccgtagaatc
      121 atccccgccc gactgaagcc ggaaaaatgg atgccggagc aaatcagtct aacgtttgac
      181 cataaaacca ttcgcatgct gatcgggatt gatgcagtga ttatcatcat cgaactgatg
      241 gtcatctcca ccctgatcat cagttatatg ctcagaccaa ctggcgatac agccctggga
      301 gcgatcataa ccagcggtta tgctttcgga ttctggaccg gggtggtcct gatcggcctg
      361 gcgatccctt tgctgttggg catactggaa ctgcgggatc ggttaaccgt gaaaatgaac
      421 cgcattgtgt ccatcgttga gccgttaatg gtgctgaccg ggggctttat cctcagatgg
      481 gtcgtagtct acggtgggca gacggtatat ccgattttga cttattaccg atgatttgta
      541 tgacccgatg ggactgaacg ggcagaaaaa ccaaacagcc gatgggcaga ataccatcgg
      601 ctgttatcat taagaggaag cgttttacgc gttcagcaat tccttttttt ctccgttcca
      661 acaaagaccg ctattgttgg cgtgagtaag aaaacggata aagcggcggg tgatgtggga
      721 cggttcctca tcggcgcggt aaaccaggtt gacatcaaga aaaagttctt caatttcctt
      781 gatttttact tctttgaggg ctcccgcatt cagctccttg gtcacagcga tgcgcggtac
      841 aatggatacg cccattccgc cttccaccgc cgctttgacc gagtggatgc tgttcatttc
      901 ggaaacgatg ttgaattcat ataaatcata gcccaaatcg ctgaaagtgc tctttagaat
      961 cgcaggaatg gcagcaccct tttcgcgcat gacaaaaggt ttctccttga gatcacgcaa
     1021 tcgaatttcc ttgcggcatt taaaagcatc ggtattggga aaaaccagta ccagttcccg
     1081 gcgatcgaga aactgggaga ccactcccgg gaaattgatc tgaccttcca cgacggccac
     1141 gtcgatttcc ttgttgagca gctttttcaa agtggcgttg gtattgtcaa tatccagttt
     1201 gattcgggat gcaggatact tggccttgaa agcctggatg ctgcagggca tggcaaaatt
     1261 acccgctgta caactggcac cgatcgacag gctggagcag tataaatcat tgatctgctg
     1321 cagatcagtg tccatcgact gatgcaggct gatgagccgg tcagcatacg ccagcacaac
     1381 tttacctgcc tcagtcaggg tgaccccttg ggcggtgcgg ttaaaaaact ttactttgta
     1441 ataatcctcc atggcttgta ccttgctgct gatggctggt tgagataaat gcaacagccg
     1501 ggctgctttt gaaaagcttt tggtttcagc aatcaccggg ataatttcca actggttgaa
     1561 aaacatctgt ccacctccct taatgatcaa aacctacaac cctttcttct ccaaccaacg
     1621 aaacatgaga attccgcctg ccaccatcac agcaatgaca aaccagtgat tgatccccaa
     1681 ggcatccgga atggtaaggt tgcccatatt gccccaggtc agcaccgttt ctgccaggaa
     1741 cggatagatt tcagcataaa tgaaagcccc ggcaaacaaa cccaaaattc ccaccaaggc
     1801 gtccagacga ccttcaccca aagcccccca ggaagtcccc gggcaataac caagcagggc
     1861 ccagccaatt ccgaacaggc cgccgccgat caaagttgcg ccaataatca gagggcgacc
     1921 ggagattttc agataaccta catcacccaa caaatagatt cctgccatac caaccaaaat
     1981 agctgtaaac ataaatttga cgatggtcat gtcctgcaat aacaaggccc cgatttgttt
     2041 atcataacgg attacacgcg cccgctgcat aatgaatccg aacaatatac ctgcaatcag
     2101 tccatataaa agattcatta ttattttccg cctcctttgt aaaccagctt tacggttatg
     2161 atgcctgctg caaaaaacat gatcatggca accagtgcac tgagggacaa caaaatcatg
     2221 gcactcatct ggccggaagg gcaaccgcct gccaaacggg ctccgatcat ggcaatcatg
     2281 ccgccaagaa atgcaaacaa tccgcgttta acataggaag tgcctaaatg gtttttccat
     2341 aaatcgggga aaaaccgcca cttaaattca ccaaatagcg ttgctcctaa aaaaccacca
     2401 ataaaaattc ctgtaacaaa caggatttgc cagcttataa ccgggttaac cactttgaag
     2461 tattcggttt gggctacctg aaaaggagat atgaactttt ccagcatccc cagcaactgt
     2521 acgatggaag tagaagtccc aaaataatta ccacttactg cgacagacag aataatcaac
     2581 agtcccgtca gtgcgccgcc aatgtatgga ttcaagccat ttttatgatc accgttcatt
     2641 gtaaccaccc tccctttcct tgaatacccc cagtgggtat aattcatttt aacaaaatca
     2701 tataatatgt caatatgtca tagaagaaca cgaaccgaag agagcaggtt tcaatcttga
     2761 taatatctta acatttgttt gatattgtca tgataattgg tgggtaagat tgctttaggg
     2821 ttcaaaataa acttaaaaga ttaatggtaa ggaggaattg ataatgaaat caagatggac
     2881 gaaactttgg gtattgggta tggccctgtt gctggttggc ggaatggctt tcagcgcggt
     2941 cgcggctgac agcaacaaca caaccacagg tctgctgcag caggggatca aagcaggtca
     3001 gcaaagagcg cagacagcgc tgaaagcggt tgtggaattg accggtttga gtgcagatga
     3061 agttcgtgcc cagagagcag ccggaagttc attggcaggg attgctgttg acaaaggcat
     3121 cagtgaacag accgtcatcg acaaagttgc agcagaacgt acggccgtac tcgatcagct
     3181 caaagccgat aacaaaatta ctgctgacca gtacaatgcc tgcgtcacca acatgcaggc
     3241 caaaatcaag gccaacattg aacgcaccgg gatcggccct gccaatggcg ggcaaggcca
     3301 gggcggcgct tatatgcgtg gagccggaca aggccagggc gcaggtgcgg ggcaaggtaa
     3361 tggacgcggt atgggccaag gccagggaat gggtcggggc gcaggagcca accaggccaa
     3421 ttgcatctat aatgtacccg ctgccaacta atacgatctt cccaaagggc gccttttacc
     3481 gggcgccctt aagctttctg tacaggttcg caaattgatt cacagcgagg ctttgtttat
     3541 ggaatatatg ggcaatgcta taatgaaaaa aagttcgttc atggggagtt atttatggag
     3601 acggttattt tactggtgga agatgaaaag aaactgcgtg acatgctcat agcccatctg
     3661 caacaggagg gttttgtggt tgtgcctgca gctgacggta tggaagcgct gcacatctgg
     3721 caggagataa aacctgatct gctggtactt gatcttatgt tgccgcggct gtcaggctgg
     3781 gaagtcctta aaataattcg ccaaaaagac aataccccag tgctcgttct gactgcccgg
     3841 gcagaggaag tagacaaact attgggtttg gaactggggg cagatgacta catgaccaaa
     3901 cctttttcgc cccgggagct cagtgcacgc atcaaggcca tcctgcgtcg cagccgcccg
     3961 accgtacaaa atacgcagac cgttttccgc aatcttcgta ttgattcgga aagaatggaa
     4021 gcttatgtgg gtgacatgct gctgccgttg accgttacgg aatttaaaat tttgttgttg
     4081 ctgacagaca atcctggaca ggtcttttcc cggttacaaa tcctggaaag ggttttcggg
     4141 gatatgtatg aaggttacga acgaaccatc gatacccata tcagcaacct tcgtcacaag
     4201 attgaggctg ccgccatcag tcaagtgagt atcaagacag tttacggcgt gggttacaaa
     4261 atgacggcgg gggaataggc atggcaaaag ctggtctggc taaaaaaata accaccaccc
     4321 tgattacggt tgcgattatt tccgttttga tcacagcgat accaatgctg ttgatgacgc
     4381 gtcagcagtt ttccaattat atcggcaaat acgatcaggc gatgttacaa caatggcagc
     4441 cgattgttgc tgaatattac gctcaaaacg gcagcaacgg actgcaggat ttgctggaat
     4501 ccaacccgat gggtggcagc agtatgggaa acggcagagg tatgcgccgg ggagaggccg
     4561 gcatgcggag caaacagggg caacgcctgc tcgtaacgga tatgaaggga atcgtcctgg
     4621 ccgactccaa aggagtactt ctgggacagc aagctgaatt tgacccggcg gatgtcagct
     4681 ccgctccgct gctggcagaa tatcagcaaa tcggcacgct gtatgtgatc agtccattag
     4741 gttccggact ggcttcactg gagaacgatt ttataaccag actgaccggc agcacagtgg
     4801 tgctggctat tttcatggcg atgatggcac tgatcctggg gcttttgctg ggaaaacggg
     4861 tgagcgaacc gctgggagaa ttgtctatag ccattcataa tgtagcccgg ggcaaacttg
     4921 atcagcgact cgatttacat ggagacgagg agtttgaaca actggggcat gattttaacc
     4981 tgatggcgca aaacctggag catgccga
//
LOCUS       DAOTHZ010000113         4928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_20359_length_4928_cov_7.706136, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000113 DAOTHZ010000000
VERSION     DAOTHZ010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20359_length_4928_cov_7.706136"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..568)
                     /locus_tag="P5549_09750"
     CDS             complement(122..568)
                     /locus_tag="P5549_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13395.1"
                     /translation="MADAIVRGEIDGINQAEFRATLTCTRGRPTGSIRGEIEINSRPR
                     DIEFSFRSNNPTRMILRKYGITRYVYAVFTQVTLTNRRTGFTIRNARAVLTARKLANG
                     RRRATLLIYRPGRTALRASGVLEDGRVFVARNVSCQLLLQSSLQQE"
     gene            complement(715..1227)
                     /locus_tag="P5549_09755"
     CDS             complement(715..1227)
                     /locus_tag="P5549_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13396.1"
                     /translation="MQNRNSGQQGFGLIKLMIKMAMLTVVLMLVAITVPKVIQSLGYS
                     QGQGLVSDVKGMIATINASKVADNVIESSVDDDLPVYKQVINYVPDYATSANLQSIIN
                     AIDFYKLANNGQLPQSDEELESYLSKPIASLGPAGATYAITYFPSLILIEATSPSSKI
                     YWAEVEAGAE"
     gene            complement(1403..4126)
                     /locus_tag="P5549_09760"
     CDS             complement(1403..4126)
                     /locus_tag="P5549_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4127 family protein"
                     /protein_id="HRW13397.1"
                     /translation="MKRKILSAILCLTLLLGGVPIAAAATQTDDTARTRTSMTIPAES
                     GPGWEVDDTPLLTAAEENNTALQPTPAEPEADVVNPEEPVPGNAAEEVAVPSDTPLPR
                     PAEAVLAYVPLDNRPVNVDRVLYEAEAAGFKVLMPAEDLYATRLDGQPLNANGTPYGD
                     SQKLMEWILAMDPSTDYFVISLDQLLSGGLVNSRVITSTDLEQEEQMIDQLIALSQNN
                     HVYIIDTVVRLAACTVGYKSATLETYNYLRQYHATPRKGLTAERLTIENIVNGYRYND
                     KGSVISTKSAYANAVRNSLLVRERKLRLIDLLLSRDDSGGIKFFIGIDDSNAQNTVQT
                     NEINYIRSLMGERGFIYSGTDELGMMAVLRLMIDHYGSNVRVAPVYFGDTEDDSAGSI
                     YDMETVQENLEEHLRSISVTMAAAEEADLQILVLTLPAKSVMNSKYISALIDRINENV
                     AGGIPTIVINAAPYAYSSNLEYRMVRECEMSMLLAYSSWGTVGNAIGLALGNGISRYL
                     YLHSCSRSSDQADMAFLKGLVFAYEKDISYLRGGGQTLFNDFLAAQGWSPSNFYKNQA
                     QVLQARLGLEYILKTHEYNVTVSDIVNNLTDCRYLKGLGGASGIIGKISLSNYTAPFF
                     RSNEIRFDINVQIGDATIHHFNQAMTISMPYRPPAGQLVYFLRLYGRDQSGKLHRIPS
                     MYDQVAGQVQFSANHQYDSLFIDTLSMDAERAYSLFADTDQSAWYFHDVLYVYEKGLM
                     KGMAEHAFKPDTPMTRAMMVTTLWKMAGAPPAASGVEFSDIAADSWYKPAIEWAAANK
                     IASGYAGGNFGPDDPVTREQLAVLLWRYAEYAGLDMAAGEYPGITTYADADAVAEVMR
                     SGFDWVCSNGIMTGTVYDGTTLLLPQDPATRAQAAAVLKRYVEMGISLPRL"
     gene            complement(4304..4546)
                     /locus_tag="P5549_09765"
     CDS             complement(4304..4546)
                     /locus_tag="P5549_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13398.1"
                     /translation="MQNRIISPLLIVFIILTAIVIFGLLHPGIPSYLIVLAALLLASL
                     LMVLHLLLAWRRLTVPYRLSILLCVIAWLAMFYGLV"
     gene            complement(4768..4854)
                     /locus_tag="P5549_09770"
     tRNA            complement(4768..4854)
                     /locus_tag="P5549_09770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4818..4820),aa:Leu,seq:taa)
BASE COUNT         1183 a         1284 c         1249 g         1212 t
ORIGIN      
        1 ggtaaggcaa atttgacttt agaagcatta caaaacactg caggctgagc ccgcagcgtt
       61 ttgtttggta attacggttt gtttattcgc ggtaccggat tttgattatc atggagttcg
      121 tctattcttg ttgcaaggat gactgcagca acagttggca ggaaacattg cgggcgacaa
      181 aaaccctgcc atcctcaagt actcccgatg ctcgcaaagc agtccttccc gggcggtaaa
      241 tcagcaaggt ggcccggcgt cgtccgttgg caagcttgcg ggcggtcagc accgcccggg
      301 catttctgat ggtaaatccg gtacggcggt tggtcagcgt gacctgcgtg aatactgcat
      361 agacataacg ggttatgcca tatttcctga ggatcatgcg ggttggattg ttggaacgaa
      421 aggaaaattc tatatctctg ggtcgggaat tgatttctat ttcacctcgg atcgatcctg
      481 tcggcctgcc gcgcgtgcag gtcaaagtgg cccgaaactc tgcctggttg atcccgtcaa
      541 tctctccgcg aacaattgca tctgccattt gatcacctct cctttatgca agaatatctc
      601 ttccctgcat agtatggtta tcatcggcca gaggtgatga tattatggat ttagttgttt
      661 ccagatcgaa gaaaagagcc ggtgaaaact gttgccgcca gagtttattt gagatcactc
      721 tgctccggct tccacctctg cccagtaaat cttggaggat ggagacgtgg cttcgatgag
      781 gataagggaa ggaaaatagg tgatagcgta agtcgcgccg gccggtccca gggaagcgat
      841 cggtttggac aaataacttt ccagttcctc atctgattgc ggtaactgtc cattgttggc
      901 aagcttgtag aaatcgatgg cattgatgat gctttgcaag tttgcagacg tggcatagtc
      961 cggcacataa ttgataacct gtttatacac tggcaaatca tcatcgacac tcgactcgat
     1021 gacattgtcg gcaactttgc tggcattgat ggtagcaatc atacccttca catcactgac
     1081 caacccctgc ccttgtgaat agcccagaga ttgaattact ttgggaacgg tgatggccac
     1141 cagcatgagc acgacggtca acatagccat ttttatcatg agtttgatca agccaaaacc
     1201 ttgctgcccg ctgttacgat tctgcatttc ccttctcccc tatcataaat gctgaaaaga
     1261 aaatgaaagc tttacacttc cgattgccga agctcaagac atctttgggt tgcccggcat
     1321 gaacgggtat tatgtaatat catatcataa tcggttattt aggtagggac aaaaattctg
     1381 ttcttttcaa acctctcctg ccttaaagac gcggcagcga aatccccatt tccacatagc
     1441 gtttcagtac ggcggcggct tgcgcgcggg ttgccggatc ttggggcagc agcagggtgg
     1501 ttccgtcata aaccgttccg gtcatgatac cgttgctgca tacccagtca aatccactgc
     1561 gcatgacttc agcaacggca tctgcatccg cgtaggttgt aattcccgga tactccccgg
     1621 cggccatgtc aagaccggca tattcggcgt accgccacag cagaaccgcc aattgttcgc
     1681 gggtaaccgg atcatcgggg ccaaagtttc ctccggcata gccggatgcg attttattgg
     1741 cggcagccca ttcaatggcc ggtttgtacc aactgtcagc ggcgatgtcg gagaattcca
     1801 caccacttgc tgccggcggc gctcccgcca tcttccacag ggtcgtgacc atcattgcac
     1861 gggtcatcgg cgtgtcgggc ttgaaagcgt gttcggccat gcccttcatc aaaccttttt
     1921 cataaacata cagcacatca tgaaaatacc aggctgactg gtctgtatcc gcgaacagtg
     1981 aataggcccg ctcagcatcc atgctcaagg tgtcgatgaa caggctgtca tactgatggt
     2041 tggcgctgaa ttgcacctgc ccggcaacct ggtcatacat actgggaatc cggtgcagct
     2101 tgccgctttg atcccgtccg tacagacgca ggaaataaac cagttgcccg gcaggcggac
     2161 gatagggcat gctgatggtc atggcttgat tgaaatgatg aatggtggca tcaccgattt
     2221 gcacattgat atcaaagcga atttcattgc tgcgaaaaaa aggcgcggta tagttgctca
     2281 gactgatctt gccgataatc ccgctggctc cgcccagccc tttcaaatag cggcagtcgg
     2341 ttagattgtt gacgatatca ctgacggtga cattatactc atgcgttttc agaatatatt
     2401 ccaaaccgag tcgcgcttgc agcacctgag cctggttttt ataaaagttg ctgggcgacc
     2461 atccctgggc ggcgagaaaa tcattaaaca gcgtctgtcc cccgccgcgc aggtagctga
     2521 tgtctttttc ataggcaaaa accaaaccct tcaagaaggc catgtcggcc tggtcactgc
     2581 tgcgactgca gctgtgcagg tacagatagc gcgaaatgcc gttgcccagg gccagtccaa
     2641 tggcattgcc gaccgttccc caactgctgt aggccagcag catgctcatc tcgcactcgc
     2701 gtaccatccg gtattccaga ttgctgctgt aggcataggg cgccgcgttg atgacaatgg
     2761 ttggaatccc cccggcgaca ttttcgttaa tccgatcaat taaggcactg atgtacttgg
     2821 aattcatcac cgatttggca ggcagggtca gcaccagaat ctgcaggtcg gcctcctctg
     2881 cagccgccat ggtcacgctg atgcttctga gatgttcttc aagattttcc tggaccgttt
     2941 ccatatcata aatggatccc gccgaatcat cttcggtatc accgaaatag accggggcca
     3001 cccgaacatt ggaaccatag tggtcgatca tcaagcgcaa aaccgccatc atgcccagtt
     3061 catcggtgcc tgaataaatg aatccccgct cccccatgag acttctgata tagttgatct
     3121 cattggtctg gacggtgttt tgcgcgttgg agtcatcaat cccgataaag aatttgatcc
     3181 cgccggaatc atcccgggac aagagcaaat caatcaggcg aagcttgcgt tcccggacca
     3241 gcagggagtt cctgaccgca ttggcatatg ctgatttggt gctgatcaca gaacccttgt
     3301 cgttataacg ataaccattg acaatgtttt ctatcgtcag acgttctgcg gttaacccct
     3361 tcctgggcgt ggcatggtat tggcgcaggt aattgtaggt ctccaaagtt gcacttttgt
     3421 agcccactgt acaggccgcc agacgcacaa ccgtgtcaat gatatagaca tggttgtttt
     3481 gggacagggc aatcagctga tcaatcatct gctcttcctg ctccaggtcg gtgctggtaa
     3541 tcacccggga attcaccagt ccaccggaga gcagctgatc gagagaaatc acaaaatagt
     3601 cggtggacgg gtccatagcc aggatccact ccattaattt ttggctgtcg ccgtacgggg
     3661 tgccgttggc attgagtggt tgtccgtcca gacgggtggc atataaatct tccgccggca
     3721 tgagcacttt aaagccggcc gcctcagctt catacaatac ccggtcaaca ttcaccgggc
     3781 gattgtccag cggaacatag gccagcaccg cttctgccgg acgaggcagc ggagtatccg
     3841 acggcactgc cacttcctct gctgcattgc cggggacagg ttcctccggg ttgactacat
     3901 cagcttcagg ctcagctggg gtgggctgca aagccgtgtt attttcttcg gcggcggtca
     3961 gcaaaggagt gtcatcgacc tcccaaccgg gtcctgactc tgccggtatg gtcatactgg
     4021 tccgggtccg cgctgtatca tcggtctgcg tcgccgcagc cgcaatgggc accccgccca
     4081 acagcagggt caggcacagg attgcactca gtatctttct tttcatggca gggatccttt
     4141 ctgttgaaag cattgttttc gtctattata ttataacctt cccggtccgg atccttacac
     4201 cgtcggccga attttgccgg cggatatttt aaaataggcg ccctgcctgg caaagcgctt
     4261 aatcaaaacg cagtacatcg tatctgtctg atcgacaaag cgcttaaacc aaaccgtaaa
     4321 acatggccag ccaggctatg acacacagca gaatgctcaa gcgatagggc accgttagcc
     4381 ttcgccaggc cagcagcaaa tggagcacca taagaagtga ggccagcagc aaagccgcca
     4441 gaacgattaa gtaggaaggg atgccgggat gaagcaggcc aaaaataacg atcgccgtta
     4501 gaatgataaa aacgatgagc aagggggata ttattcggtt ttgcattttt cttcagtccc
     4561 cctttggttg atgcgaaatc cttagatcaa tcaactcgag cataccatca gctatcgcct
     4621 tcctgtttat tatgctgcat ccttcgtcaa tcagcaatat cacacgggag caatacgaat
     4681 aaataccata aaatcaacat gctatctaca cggggtcaag gcaaaatgaa aaaagctgag
     4741 atacaatctc agcaaagtca gtaatgttgg tgcccgaggc cggggtcgaa ccggcacgat
     4801 attgctatcg cgggatttta agtcccgtgc gtctgcctat tccgccaccc gggcagagcg
     4861 ttcgagatga aaatatggag ggcgagaccg gattcgaacc ggtgaatggt gattttgcag
     4921 accacagc
//
LOCUS       DAOTHZ010000114         4832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_20890_length_4832_cov_7.892192, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000114 DAOTHZ010000000
VERSION     DAOTHZ010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20890_length_4832_cov_7.892192"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /gene="infA"
                     /locus_tag="P5549_09775"
     CDS             <1..186
                     /gene="infA"
                     /locus_tag="P5549_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRW13399.1"
                     /translation="KVLEPLPNAMFTVELENGHKVLAHISGKMRMNFIRILPGDRVMV
                     ELSPYDLNRGRITYRFK"
     gene            215..328
                     /gene="rpmJ"
                     /locus_tag="P5549_09780"
     CDS             215..328
                     /gene="rpmJ"
                     /locus_tag="P5549_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRW13400.1"
                     /translation="MKVKPSVKPMCEKCKVIKRNGKVMVICENPKHKQVQG"
     gene            401..769
                     /gene="rpsM"
                     /locus_tag="P5549_09785"
     CDS             401..769
                     /gene="rpsM"
                     /locus_tag="P5549_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRW13401.1"
                     /translation="MARIAGVDLPRDKRVEISLTYIYGIGKASSQKILAEAGINPDTR
                     VKDLTEEEVSKLRAIIDKSFTVEGDLRRDVSMSIKRLMDLGCYRGIRHRRGLPVRGQK
                     TKTNARTRKGPKRTAGGKKK"
     gene            789..1184
                     /gene="rpsK"
                     /locus_tag="P5549_09790"
     CDS             789..1184
                     /gene="rpsK"
                     /locus_tag="P5549_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRW13402.1"
                     /translation="MARRTTTTRVKRREKKNIAEGIAHIKSTFNNTIISITDKHGNAI
                     SWASAGTSGFKGSRKSTPFAAQQAAETAARAAMEHGLKEVECYVKGPGAGREAAIRSL
                     QAAGLEVSVIKDVTPIPHNGCRAPKRRRV"
     gene            1200..1829
                     /gene="rpsD"
                     /locus_tag="P5549_09795"
     CDS             1200..1829
                     /gene="rpsD"
                     /locus_tag="P5549_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRW13403.1"
                     /translation="MGRYTDSVCRQCRREGEKLFLKGDRCYSAKCAIEKRPFVPGQHG
                     QKRKQKTSEYGLQLREKQKTKRIYGVSEKQFRNSFKKADRQSGITGDNLMILLERRMD
                     NVVFRMGLASSRKEARQLVNHDHFTINGKKANIPSMLVKAGDFIQVKESSASSPKFED
                     LKATAAYKTAPEWLEVDVENLSGKVLALPSREQIDTVVSEQLIVELYSR"
     gene            1876..2826
                     /locus_tag="P5549_09800"
     CDS             1876..2826
                     /locus_tag="P5549_09800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRW13404.1"
                     /translation="MLEMEKPRIERVEQDPSTNYGKFVIEPLERGYGTTLGNALRRVL
                     LSSLPGAAVTSIKIDGVLHEFSTIPGVLEDTTELILNIKQMVLRYDGNERKTVRFEQK
                     GKKDVCAGDLEPDAEVEVLNPELHIASLDADGILEIEMTVERGRGYVGADQQPHKNDD
                     IVGLLPIDSIFTPVTKVNYTVDNARVGKRTDFDKLVLEVWTNGSIKPEEAISLSAQIL
                     IQYLSLFTEIDDTYAEVEILVEKEEEKKDKVLEMSIEELELSVRASNGLKRASINTVG
                     DLIEKTREEMSKIRNLGQKSLEEIERRLNEMGFTFKKSED"
     gene            2842..3183
                     /gene="rplQ"
                     /locus_tag="P5549_09805"
     CDS             2842..3183
                     /gene="rplQ"
                     /locus_tag="P5549_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRW13405.1"
                     /translation="MSYRRFGQLADHRRSMMRNAVTSLLEHGSITTTETRAKDIRKVA
                     EKMITLAKKGDLHARRQAESYLMKEEVVYKLFNGINERFSNRDGGYTRIIKTGYRKGD
                     GAPMVIIELVD"
     gene            3363..4112
                     /gene="truA"
                     /locus_tag="P5549_09810"
     CDS             3363..4112
                     /gene="truA"
                     /locus_tag="P5549_09810"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRW13406.1"
                     /translation="MPKIKLVMEYDGSHYHGFQKQRNAHTIQAELEQAIYHISRESVT
                     VIAAGRTDAGVHALGQVASFHTGMEFPADRWRPALNSVLPGDVRILKSELVSASFHPQ
                     YDSLYKSYQYLIYRKKERAVFYRQYALLCDQALEIEVMQEACHQIIGRHDFRCFCASG
                     SGAKTWERTIYCCRLEEEGDWLKLHITADGFLYNMVRIIVGTLLEIGRGRMKPGQITN
                     MIASCERSQAGPTAPPQGLYLCSVNYPSSAE"
     gene            4308..4730
                     /gene="rplM"
                     /locus_tag="P5549_09815"
     CDS             4308..4730
                     /gene="rplM"
                     /locus_tag="P5549_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRW13407.1"
                     /translation="MAKPAEIDRKWYVIDAEGQTLGRLASEVASILRGKHKAIYTPHV
                     DTGDFVIVINAGKIEMTGNKLSDKMYRTYSGYPGGLKEVNYGTLLARKPEKAIEAAVK
                     GMLPHNRLGRAMYKKLKVYRGSEHPHQAQKPELRELRG"
     gene            4741..>4832
                     /gene="rpsI"
                     /locus_tag="P5549_09820"
     CDS             4741..>4832
                     /gene="rpsI"
                     /locus_tag="P5549_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641672.1"
                     /note="forms a direct contact with the tRNA during
                     translation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRW13408.1"
                     /translation="MASVQYWGTGRRKTAIARVRLVPGSGNINI"
BASE COUNT         1520 a          990 c         1220 g         1102 t
ORIGIN      
        1 aaagtactgg aaccattgcc taacgccatg tttacggttg aactggaaaa tggccataag
       61 gtcctcgccc atatatcagg gaaaatgcgg atgaatttta tcagaatttt acctggcgat
      121 cgagtaatgg ttgagctgtc gccgtatgat ttgaaccgcg gcagaatcac atatcgtttc
      181 aagtagcttt ccaccgcaaa ggaggtatcg tttcgtgaaa gtaaaaccat cagtaaaacc
      241 catgtgtgag aaatgcaaag tgattaaacg gaatggcaaa gtaatggtta tatgtgagaa
      301 ccctaaacac aaacaggttc agggataatc aggcggattg cgtgattgaa gatggaaagt
      361 caaacataaa aatcattaaa actgaaggat gtgaaaaaat ttggcaagaa tagcaggtgt
      421 tgatttaccc cgtgacaaga gagttgaaat ctcactaacc tatatctacg ggatcgggaa
      481 agcatcatca caaaaaatat tggctgaagc aggtataaac ccagatacga gggtaaaaga
      541 tttaacagag gaagaagttt ccaagcttag agctattatt gacaagagtt ttaccgtcga
      601 aggcgacctg cgcagagacg tcagtatgag tatcaagcgc ttgatggatc tgggttgcta
      661 tcgtggtatt cgccatcgtc gcggattacc agtgaggggt cagaaaacca aaaccaatgc
      721 ccgtacccgc aaaggaccca aacgcactgc cggcggcaag aagaaatagg attaaggagg
      781 gtaattgagt ggcacgaaga acaaccacta cacgcgttaa acggcgtgaa aagaagaaca
      841 tcgcagaagg aatcgcacat atcaagtcaa cttttaataa tacaattata tccattaccg
      901 ataaacacgg aaatgcaatt tcctgggcca gcgctggcac atcaggattt aaaggttccc
      961 gtaaaagcac cccgtttgct gcccagcaag cggctgagac tgcagctcgt gcagcgatgg
     1021 agcatggtct caaagaagtc gaatgttatg taaaaggacc tggcgccggt cgtgaagcag
     1081 caatccgttc tctacaggca gcgggtttgg aagttagcgt tatcaaagat gttacgccca
     1141 ttccccacaa cggctgcaga gcgccaaaaa gaagaagggt ataaggaggg aatctttaag
     1201 tgggaagata tacagattca gtttgccgcc agtgccgtcg cgaaggtgaa aaattgttcc
     1261 tcaaaggaga tcgctgttat tcagccaaat gtgcgattga gaagagacct ttcgtaccag
     1321 gtcagcatgg acaaaaacgg aaacaaaaga ccagtgaata tggtttgcag ttaagagaaa
     1381 aacaaaagac caagcgaata tatggagtaa gcgagaaaca gttccgcaat tcctttaaaa
     1441 aagccgatcg tcaatcgggc attaccggtg acaatctaat gattttactg gaaagacgga
     1501 tggataatgt tgttttcaga atgggtctgg ccagttctcg caaagaggca cgccagctcg
     1561 ttaaccatga tcatttcacc atcaacggca agaaggccaa tataccttcc atgctggtga
     1621 aagccggtga ttttatacaa gtcaaggaga gcagtgccag ttcacccaag tttgaagatc
     1681 tcaaagcaac tgcagcctat aaaactgccc ccgagtggct ggaagttgat gtggagaacc
     1741 tcagtggaaa agtattggct ctgccgagca gagagcagat agatacggtg gtcagcgaac
     1801 agctcatcgt tgaactatac tccagataat ctatctatat ttttttacct ctaacagaca
     1861 aggaggggat aattagtgct agagatggaa aaacctcgga tcgagcgggt agaacaagat
     1921 ccttcaacca attatggcaa atttgtgatc gaaccactgg agagaggcta cggtacaact
     1981 ttaggcaatg cgctgcgtcg ggtactgttg tcttcattgc caggcgcggc cgttacgtcc
     2041 ataaagattg acggtgttct gcatgaattt tccaccatac ctggcgttct ggaagatacg
     2101 acggagctga tactgaacat caagcaaatg gttttgcgtt atgatggcaa tgaacgcaaa
     2161 accgtccgtt ttgagcaaaa aggcaaaaaa gacgtttgtg caggtgatct ggaaccggac
     2221 gcggaagtgg aagtactcaa tccggaacta catattgcca gcctcgacgc ggatggcatt
     2281 ttggaaattg aaatgaccgt tgaaagaggc cggggatatg tcggtgctga tcaacagccg
     2341 cataaaaatg atgatatcgt aggtttgctg ccgatcgatt cgatatttac accggttacc
     2401 aaagtcaatt atacagttga caatgcccgg gtgggtaaaa gaacagattt tgacaaactg
     2461 gtgctggaag tctggaccaa cggcagtatc aagcccgaag aagccatcag tttatcagcc
     2521 cagattctga tccagtatct cagcttgttt accgaaattg acgataccta tgcagaagtt
     2581 gagattttgg ttgaaaaaga agaagagaaa aaagacaagg tattggaaat gtcaattgag
     2641 gaactggaac tgtcagtacg tgcttccaac ggacttaaac gggccagcat caatacagtt
     2701 ggcgatttga ttgaaaaaac ccgggaagaa atgagtaaaa tcagaaactt ggggcaaaaa
     2761 tcgctggaag aaattgaacg cagactaaat gaaatgggat ttacgtttaa aaaatctgaa
     2821 gattagaaag gaggatcatc cttgagttac cgtagattcg gacaattagc ggatcaccgc
     2881 agatccatga tgagaaacgc tgtcacctcc ttgctggaac atggcagtat tacgaccacc
     2941 gagaccagag ccaaggacat cagaaaagtt gcggaaaaga tgatcacttt ggctaaaaag
     3001 ggcgatctgc atgccagaag gcaggctgaa tcctatttga tgaaagaaga agtggtttac
     3061 aagcttttca acgggataaa tgaacgcttt tcaaatcgtg acggaggata cacccggatt
     3121 atcaaaaccg gttatcgcaa aggagacggg gcccccatgg taatcatcga gctggtcgac
     3181 tagaaacaga cgagccgatt caggatcatt aagactaaaa taagagttga tatcactctt
     3241 caactgatat catgggagga tttagcaata tcctcctaat tttttgtagg ctatctgcct
     3301 ggagacggcg gtgctggcaa ggcacgggta ccattcatgg cgcagaccga ggagatgaat
     3361 cgatgccgaa gatcaagttg gttatggaat atgacggcag tcattatcat ggctttcaaa
     3421 aacagcgcaa tgcccatacg attcaagccg aactggagca ggccatttat catatcagtc
     3481 gggaatccgt cacagtcata gctgccgggc ggaccgatgc aggtgtccat gccttgggac
     3541 aggtagcatc tttccatacc gggatggaat ttccagctga tagatggcgt ccggctttaa
     3601 acagcgtttt gccgggcgat gtccggatac tgaaaagcga attggtatcc gcttcttttc
     3661 atccacaata tgattccttg tacaagagtt atcaatattt gatttatcgg aaaaaggaac
     3721 gggcggtatt ttatcgccaa tatgcgttgc tctgtgatca agcactggag attgaagtga
     3781 tgcaggaagc ctgccatcaa atcatcggca ggcatgattt ccgttgcttt tgtgccagtg
     3841 gttcgggggc caaaacctgg gagagaacca tatactgctg tcggttggaa gaggaaggag
     3901 actggctcaa gctgcatata accgcagatg gttttttgta taacatggtt cgcattattg
     3961 tgggaaccct gttggaaatt ggccgcggac gtatgaaacc tgggcagata acgaacatga
     4021 tcgcatcctg cgagcgtagc caggccggtc caaccgcccc cccgcagggt ctttatcttt
     4081 gttcagttaa ctatccgtca tcagcagaat aaaatcccaa accattcagg tgaaaagcaa
     4141 agcaatcaga agcatttctt gacaaactcc tttaaatatg gatacaataa taaggcagtc
     4201 tctgaactcc tgtttggctc cagccccgtt gacaagcaga gagcacggag caattgtttg
     4261 tgttcgcaac ccattaacaa caggagggaa tttctttgaa aacatatatg gcgaaaccag
     4321 cggaaattga ccgcaaatgg tacgtgatag atgcagaagg ccagacgctg ggcagacttg
     4381 cttcagaagt cgcctccatt ctaagaggaa aacataaagc catttataca ccccatgttg
     4441 ataccggtga ttttgtgatt gtaatcaacg ctggcaagat tgagatgacc ggcaacaaac
     4501 tcagtgataa aatgtaccgc acttattccg gttatccggg cggattgaaa gaagtcaact
     4561 acggcaccct gttggccaga aaacctgaga aggcaattga ggctgcagtc aaagggatgt
     4621 tgccgcataa ccgtctgggc cgggcgatgt acaagaagct caaggtttac agaggcagtg
     4681 aacatccaca tcaggcccaa aaaccagaac ttcgtgaact gagaggttag gaggagcgat
     4741 atggcaagcg tacaatactg gggaaccgga agaagaaaaa cagcaattgc cagagttaga
     4801 ttagtccctg gcagcggaaa cataaatatc aa
//
LOCUS       DAOTHZ010000115         4818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_20963_length_4818_cov_7.215620, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000115 DAOTHZ010000000
VERSION     DAOTHZ010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20963_length_4818_cov_7.215620"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /gene="porA"
                     /locus_tag="P5549_09825"
     CDS             complement(<1..676)
                     /gene="porA"
                     /locus_tag="P5549_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRW13409.1"
                     /translation="MKKPLSGNEAVAYALKQINPDVMGAFPITPSTEIPEYFAQYVSN
                     GEVNTEYVTVESEHSSMSVCIAAQAAGARTVSATSSCGLALMFELLYVAASCRLPITL
                     ACVNRALSGPININNDHSDSMGARDTGWIQFYAENNQEAYDNMLMAMPVGETPSVRLP
                     VMTCMDGFITSHAVENIELMETETVRRFIGEYHPQHYLLKKENPLAVGPYDVSAYYME
                     HKVLQAE"
     gene            complement(673..981)
                     /locus_tag="P5549_09830"
     CDS             complement(673..981)
                     /locus_tag="P5549_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRW13410.1"
                     /translation="MISAKDINEHTPWQELTPGGEIYEPGTARAFDTGTWRTDTPVFH
                     EEACKHCMLCVPFCPDNAIPVKDGKRLDFDYMHCKGCGICMEVCPFPAITMVSGGVVK
                     "
     gene            complement(978..1562)
                     /locus_tag="P5549_09835"
     CDS             complement(978..1562)
                     /locus_tag="P5549_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRW13411.1"
                     /translation="MASNDMVEIRWHGRGGQGAKMACMLLADVAGLEGKFVQGFPEYG
                     PERMGAPITAYNRISEQRCSIHSNIYEPDYVVVVDETLLDSVDVTGGLKKDGAIIINT
                     PHFPQQMRSRLRGWKGKVCTIDARRISEEVLGRNFPNTPMLAAVIKVSGVLETERFLA
                     HIEESFQHKFAAKPQVIEGNMATLRKSLEEVRVG"
     gene            complement(1926..2891)
                     /locus_tag="P5549_09840"
     CDS             complement(1926..2891)
                     /locus_tag="P5549_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRW13412.1"
                     /translation="MSVFLYILNHNIIPILVLVILGYLLGKKFELSIYTLSKLNFFIF
                     VPAFIFVNLYTTSIPQDMLKVVAAVILLLLLNMIMVLIYAKIRGFHEGYKNAFTNSVL
                     FANTGNIGVPLITLVFSSPPFFVNGTAPYLALALTAQVMVLVVQDTAMNTLGFFYASK
                     ANTHWKNSVLKVLKMPAIYCVLLAVILKSVPYDITLSPVWPALEYAKNALVSVALITL
                     GVQLSMTTFALKNPDAYMSALMRLLVSPVLALFVIHMLDLQGVVAQVVMITAALPTAV
                     NTALIAVECDNYPDFASQAVMISTFLGALSLVLIIFLARLLFPVV"
     gene            complement(2987..4654)
                     /locus_tag="P5549_09845"
     CDS             complement(2987..4654)
                     /locus_tag="P5549_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HRW13413.1"
                     /translation="MLNRWLRLKSGIQLNKRLTREQKIKAYQAISDLSRPTYPFYLMV
                     VLSTVIASFGLLVGSTAVVIGAMLVAPLMGPIFGIGLGLSSGDRKLIKNAVFSETIGV
                     ILAVMIAVLIGLIPIRLGLSPEILSRTQPNTYDIIIALFSGFAGAYALVDEKIGSALP
                     GVAIATALVPPLATCGICLALQQWTMAGGAFLLFFVNFLAIELAAAITYFAYGLVRIS
                     AEDKSSLRSFLQKFWLSLTILALIAVFMTRSLQQIEANRMLADDLKSAITEQVAANAG
                     ARVSDIRYQYADKKLQVMAEVLTPQEIDARQVAQIEKNLQTKIDPNIQLIVRSMISRD
                     FDNSGYVFITEDEKALRFEQDQQNLFLAQVYSAMREGLMDYRGSSLVDIQNTVRDGKN
                     HLNAIVNTPSEFAPEQVASLESFLASHLEVPVHLTIRSIITYDADATRYLYADAAGSG
                     QLEGEALVFHRQLEKALTENLKTIQPAANLLEFNYTRRADVLLIVARVRTPAVLGPDE
                     VKLMEQALQNNFDPQATLVVRSTLGADATSEWYLGNYNDPVFDVVNN"
BASE COUNT         1335 a         1244 c         1045 g         1194 t
ORIGIN      
        1 cctcggcttg caataccttg tgttccatat agtaagcact cacatcgtac ggtcctaccg
       61 ccagcgggtt ctctttctta agcagataat gctgcggatg gtattcacca ataaaacgtc
      121 gtactgtttc ggtctccata agctcgatat tctcaacggc atgactggta ataaagccat
      181 ccatacaagt catcaccggc aaacgtacac tgggtgtctc gcctacaggc atggccatca
      241 gcatattatc gtaggcctct tggttgttct cagcataaaa ctggatccaa ccggtgtctc
      301 gtgctcccat ggaatcggag tggtcattat tgatattgat cggaccggac agggctcggt
      361 tgacacaagc caaagtaatg ggcagacgac atgaagccgc gacgtacaaa agctcaaaca
      421 tcaacgccaa accgcaggag gacgtagcgc tgacggttct ggcacctgcc gcctgagcgg
      481 caatgcatac tgacattgaa ctatgttcag attcaaccgt cacatattcc gtattcacct
      541 cgccgttgga cacatattgg gcaaagtatt caggaatttc ggtggaaggg gtgatgggaa
      601 atgcgcccat cacatccggg ttgatctgtt tgagggcata agcgactgcc tcgttgccgg
      661 aaaggggctt tttcatttga ccacacctcc ggacaccatc gtaatcgccg ggaaagggca
      721 aacttccata caaataccgc agcccttaca gtgcatgtaa tcgaaatcaa ggcgtttccc
      781 gtccttcact ggaatcgcat tgtccgggca aaagggcaca caaagcatac aatgtttgca
      841 tgcctcttca tgaaaaaccg gtgtgtcggt tcgccaggtt ccagtatcaa aagctcttgc
      901 ggttcccggt tcatagattt ctcctcccgg ggtcagttcc tgccagggag tatgttcatt
      961 aatatctttg gcagatatca tcctacccgc acctcctcca aggattttct cagcgttgcc
     1021 atgttgccct caattacctg gggtttagcg gcaaatttat gttgaaagga ttcctcaata
     1081 tgcgccaaga atcgttctgt ttccaagacg ccgctcactt taatcacagc cgccagcatc
     1141 ggtgtattgg ggaaatttct gcccagaacc tcttccgata tgcggcgtgc atctatggta
     1201 cagacttttc ccttccagcc ccgcagtcgg gaacgcatct gctgggggaa atggggtgtg
     1261 ttaatgatga tcgctccatc cttttttaat cctccagtca cgtcgacgct gtccagcagc
     1321 gtttcatcca ccaccactac ataatccggc tcataaatgt tggaatgaat cgaacaccgt
     1381 tgctcactaa tgcgattata cgctgtaatc ggagcaccca tgcgttccgg tccatactcc
     1441 ggaaatccct gcacgaattt tccttccaaa ccagctacgt ccgccagcag catgcaggcc
     1501 atctttgctc cttgcccgcc tcgcccgtgc cagcgtattt caaccatgtc gttgctcgcc
     1561 attgctttct cctccagtca aacatcgcta agacttctgt aaaaagtaaa aaagctttcg
     1621 tcccgtaaag ggacgaaagc cataaagaca ttcgctatac cacccattaa gccaatcata
     1681 caacatacca tcatatgcgt tccctgtgac gggggaaagc cgtcggagcc tactgggtat
     1741 acgttcggtc cgcagctcag gagtgatttt cgggttcaga ctactggtac cgggcttgca
     1801 cctatccccg gctctctgca acgttcagct gaaacttact ctctccgtca aagcctttgt
     1861 tatttttaaa ttataaataa ctatacctac tccatggttt tttgtcaatc actgtatgtg
     1921 ctcaattaaa ccaccggaaa caggagtctt gccaagaaga ttatcagaac cagactcaag
     1981 gcccccagga aggttgaaat cataaccgct tgcgatgcaa aatcaggata attgtcacat
     2041 tccaccgcaa ttagtgcggt attcacagca gtcggaaggg cagcggttat catcactacc
     2101 tgagcaacaa ccccctgcag atcgagcata tgaataacga acagcgccag caccggactg
     2161 accaaaagtc tcattaaagc cgacatataa gcgtccgggt tttttaacgc aaacgttgtc
     2221 atcgaaagct gcacccccaa agtaatcagg gcaacggaaa ccaatgcgtt tttggcatac
     2281 tccaatgccg gccaaaccgg cgaaagtgta atatcgtaag gaactgattt caagatcact
     2341 gccaaaagca cgcaatagat cgccggcatt ttgagaactt ttaagacaga attcttccaa
     2401 tgcgtattgg ctttgctggc ataaaaaaaa cccagtgtat tcatggccgt atcttgcacc
     2461 accagcacca taacttgggc cgtcaaggcc aaagccaaat agggcgcagt tccattcaca
     2521 aaaaacggcg gactgctgaa gaccagggta atcaagggta caccgatatt gccggtattg
     2581 gcgaaaagaa ctgaattggt aaatgcattt ttataccctt catgaaatcc gcgtattttc
     2641 gcataaatta ataccataat catgttgaga aggaggagca aaataactgc cgccaccact
     2701 ttaagcatat cttggggaat gctcgtcgta tacaggttta caaaaataaa agcgggaaca
     2761 aaaataaaaa agttcagctt ggacagcgta taaatgctca actcaaactt tttgccgagc
     2821 agatatccga ggattaccag aactaaaata ggaatgatat tgtgattaag aatgtataaa
     2881 aagacgctca tcgcttgatt cctttctgcc agcactctga acacggcaga agcaaattaa
     2941 aatccattta cacagtgctg catatttttt atattatgca aagtcctcaa ttgtttacca
     3001 catcaaagac aggatcattg taattgccca gataccattc tgaggttgca tcggcaccaa
     3061 gcgttgaacg cacaaccaat gtagcctggg gatcgaaatt attttgcaac gcctgttcca
     3121 tcaatttcac ttcgtcaggc cccaacactg ctggagttct taccctcgcc acaatcagca
     3181 ggacatccgc tcgcctggta taattaaact ccaaaagatt ggccgcgggc tggattgttt
     3241 tcaagttttc agtcaaagct ttctccagtt gacggtgaaa aaccagggcc tccccttcaa
     3301 gctgaccact gcccgctgca tcggcatata ggtaacgggt agcatcggca tcgtacgtta
     3361 tgatcgagcg aatggtaagg tgtacgggaa cttcgagatg ggaagccagg aaactctcca
     3421 aactggctac ctgttccggc gcaaactcac tgggcgtgtt aacgatcgca ttgagatgat
     3481 tcttcccgtc acggacggta ttttgaatat caaccagact ggaacctcga taatccatta
     3541 aaccttccct catcgcactg taaacctggg cgagaaagag attttgttgg tcctgttcaa
     3601 aacggagcgc cttctcatcc tcggtaatga acacatagcc gctgttgtca aaatcgcgtg
     3661 agatcatgga acgaacaatc agctgaatgt tggggtcgat tttggtctgt aggttttttt
     3721 caatctgggc cacctgcctg gcatcgattt cctggggggt cagaacctcc gccataacct
     3781 gtagtttctt atctgcatac tgatagcgta tatcagaaac gcgagcaccc gcattggccg
     3841 ccacctgctc agtaatggcc gacttcaagt catccgccag catgcggttg gcttcaattt
     3901 gctgcagact cctggtcata aaaactgcga ttaaagccag aatggtgaga ctcaaccaga
     3961 atttttgcag aaatgagcgc agtgaagatt tatcctctgc ggatatcctc accagaccat
     4021 aggcaaaata agtgatggcc gccgccagtt caatggccag aaaatttacg aaaaacagca
     4081 aaaatgcccc acccgccatc gtccattgct ggagcgccag acagatgccg caggttgcca
     4141 ggggaggtac cagcgccgtt gcaatagcca cacccggcaa agctgagcca attttctcgt
     4201 caaccaatgc ataagcaccg gcaaaaccag aaaaaagcgc aatgataata tcatatgtat
     4261 taggttgggt tcgtgataaa atctccggac tcaaccccag acgaataggg atcaacccta
     4321 tcaaaacggc aatcataact gccagaataa cgccgatagt ttcggaaaac acagcatttt
     4381 ttatcagctt acgatcacca ctggacaggc ccaagccaat gccaaaaatc ggccccatca
     4441 acggagctac cagcatcgcc ccgatgacaa ctgccgtact gccaaccaaa agcccaaatg
     4501 aagcaatgac agttgataaa accaccatca aataaaacgg ataggtgggt ctggataggt
     4561 cgcttatggc ttggtaggcc ttgatttttt gctctctcgt cagtcgttta ttgagctgaa
     4621 taccagactt taaacgtaac catcgattca acataaagcg ttcctcattc cactgtcaaa
     4681 tcataaattg tcgctggtga attgttccat gccctgaacc cggtgtggtt atttgatgcc
     4741 tgtatatcac tttcaatttg aaagtctttt aacaaaacct ctccgtcaag tgacacggtc
     4801 agtagttttt ttccggga
//
LOCUS       DAOTHZ010000116         4793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_21105_length_4793_cov_8.280709, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000116 DAOTHZ010000000
VERSION     DAOTHZ010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21105_length_4793_cov_8.280709"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..1243)
                     /locus_tag="P5549_09850"
     CDS             complement(473..1243)
                     /locus_tag="P5549_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRW13414.1"
                     /translation="MEEYKSLLWEMIEPGIGLLTLNRPDYYNAVDGDMMVELENFWLR
                     RLNDEATKVIILKGAGEKGFCAGLDMKWAIKDSQANVGNVWANFRFQARLARINLAMR
                     RAPQPIIAMVHGAAAGEGFSFTLASDIRVITPDARFCAAYINIGLGGADMTCSYFLPR
                     LIGAGRAYEFMYTGEFMSSEEAWNLGLVSKIVPREELYEAALTYARKLMTKNPMGLYL
                     TKEAINMNLDAGGLEQAICMEDRNQVMLTLASGLKSTV"
     gene            1660..3588
                     /locus_tag="P5549_09855"
     CDS             1660..3588
                     /locus_tag="P5549_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRW13415.1"
                     /translation="MTKWEVRSQWKELHLKPELRNSLRPEVRDSWERCYDYNLNPQLR
                     ENPYICTRYELKQAQDNVANLIQASDRVMSSLYEFVAGTGFVVVLSDANLCLLKTVGD
                     AESLAWSDNAYLVEGSMWGEELVGTNAGCLAIALAKPISVFGYEHFCLFSHVAACSCA
                     PLIDQGRIIGGLGMVAPYSKVNSHTLGMVVAASKHIKYTMAMERAKRYHELIMDSMAE
                     GLFTLDYNGNITYMNENCARIFKLDKLRIIDHNIYEFFGHNKENHYFINKITQGHTTT
                     DENFTLIIGKETIHCNVTCNPLKNVDMTDGGSVVIVRESHHINRLVRNYIGGGAKVTF
                     DDIIAKDPKSLQYVKTAKAAASSSSNVLLLGESGTGKDIIAQAMHNASPRKNNSFLAI
                     NCAALPRDLIASELFGYEDGAFTGARKGGNIGKFELADQGTIFLDEIGDMPLDLQASL
                     LRVLEEKKVMRLGGGKLVPVNVRVIAATNKDLETEIERNRFRRDLFYRLGVIKITIPP
                     LRERPEDIMPLTENFLKKTCRRFNRPLMSLSPEVEDAFMNYDWPGNVREMQNVIEGST
                     QLAPHHEITYDLVSQYITPKAVSLPSDPGTQTVAVVEKQLILDSLAKYKYNKTEVAKA
                     LGMSRRTLYRRLNEYGIM"
     gene            3767..>4793
                     /locus_tag="P5549_09860"
     CDS             3767..>4793
                     /locus_tag="P5549_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW13416.1"
                     /translation="MIAALAVTGCGQKADDQNQSAAPEVIKVGVYEPLTGTNAAGGQM
                     TLEGIKLANQLYPEVLGKKVELVVVDNKSEKQEAANAVARLVDQDKVNVIIGSYGSSV
                     SMAGGPVAQDAQVPVIGCSPTNPAVTLGNEYYFRVCFIDPFQGTVMSNYASQTLNAKT
                     AVIIQDVQQDYSVGLSNYFEKAFKATNGDAAILAKLNYNTGDKDFSAQLTEAKSKNPD
                     VIFAPGNFLECGLMVAKAREMGINAPMLGGDTWEAQEFLDQVKDYKDIYFSTHFTAEQ
                     PVTEESTTFLNEYKKAYPDKEVNAFAALGYDAYKLALDAITRADTADSIKIRDALAQT
                     KDFQGATG"
BASE COUNT         1275 a         1220 c         1106 g         1192 t
ORIGIN      
        1 ttcttttaaa ataaatttga tatctctggt gttatatgaa tatttgtatg acactcgtat
       61 acttcctttc tgtttgcgtt ttcccacact ttattgtctt ttcccgaaat gaatcgccta
      121 caccaccggt cagccgatga tctggcgcca aacctgtcat tcatccctcc caacgatgtt
      181 gcgtataaat aacactcatg ataattacat atacgttaaa cgaatatagc ctgatataat
      241 gcaaattcgg tgccatacat caccaataat ccgctctcca gtgctccttg ttcaattttt
      301 ctcaggtacc aacccccttc gggtccccta aagaaatttt gcgccggtct tttctgcgca
      361 aatattacgt gataaaaaca acctgccaga aaacgaattt gccagattga cccgttgtgt
      421 gccaatctgg caaaatgccg ctattcattt tttttataaa tgattggtga gattatacgg
      481 ttgatttcag tccagatgcc agggtcagca tgacctggtt gcggtcttcc atgcaaatcg
      541 cctgttccaa tccgcctgca tcaagattca tgttgatggc ttccttggtg agataaagac
      601 ccatcggatt tttcgtcatc agcttgcggg cataggtcag ggctgcctca tacagctctt
      661 cccggggcac gattttgctt accaggccta aattccaagc ctcttcggaa gacataaatt
      721 caccggtgta cataaattca taagcccgcc cggcgccgat cagtcggggc aggaaataac
      781 tgcaggtcat gtctgccccg cccaagccga tattgatgta agccgcgcaa aaacgcgcgt
      841 caggggtgat gacacgaata tctgaggcca gggtaaaact gaagccttct cctgccgctg
      901 cgccatggac catggcaatg atcggctgcg gcgctctgcg cattgccagg ttgatccgtg
      961 ccagccgcgc ctggaaacgg aagttggccc atacattgcc gacattggcc tgggagtctt
     1021 tgatggccca tttcatatcc agtccggcac agaagccttt ttctcctgcc cctttgagaa
     1081 taatgacttt ggtggcttcg tcgttcagcc ggcgcagcca gaagttctcc agctcgacca
     1141 tcatgtctcc gtccacggca ttgtagtaat cgggacgatt caaagtcagc agaccgatac
     1201 ctggttcaat catttcccat aacaagcttt tgtactcttc catttcgatt tgataccccc
     1261 atttcatttt tttaattgca gtattatttc cattatacag ataccgatgc attttcgtca
     1321 gatgatttgt tatttacaac aaaaatcaac ttcacaacgc aagacagcag ccacaggata
     1381 tgacgaagtc ccatatttgt gtcattctga tacattaagg gatttcatga acccgttttt
     1441 gccaatattc tgtattctcc tttatacttg ttcgcaatat ttgtgccatg acaaatattt
     1501 caacattcct tgataaatcg ggatttcttg tcaatgctca atgaccgctt acgtcaccct
     1561 ggtttacatc atgtaccata ttgtgccgcg aagcgacaag tgctatattg aataatgaac
     1621 ggcaacgtta ttattccccg tcaaaaagga gaatcaatca tgaccaaatg ggaagtccgc
     1681 agccaatgga aagaactgca cctcaagcct gaactccgca acagcctgcg cccggaagtc
     1741 agagactcct gggaacgctg ctacgattac aatctcaatc cgcaattgcg ggaaaaccct
     1801 tatatctgta cgcgctatga attgaaacag gcccaggaca atgtcgccaa tctgattcaa
     1861 gccagcgacc gggtcatgtc cagcctgtat gaatttgttg ccggcacggg tttcgtggtg
     1921 gtattgtccg atgccaatct gtgtctgctg aaaacggtgg gtgatgctga gtccctggcc
     1981 tggtccgaca atgcttattt ggtcgaaggt tcgatgtggg gcgaggaact ggtcggcacc
     2041 aatgcgggat gcctggccat cgctttggcc aaaccgattt ccgtatttgg ttatgaacac
     2101 ttttgtttgt tttcccacgt cgctgcctgc tcctgtgcgc cgctgattga ccagggcagg
     2161 atcattggcg ggctgggcat ggtggcaccc tacagcaagg tcaacagtca cactttgggc
     2221 atggtggtgg cagccagcaa gcatatcaaa tacaccatgg ccatggaacg cgccaaacgc
     2281 tatcatgaat tgatcatgga ttccatggcg gaagggctct ttacgctgga ttataacggc
     2341 aacattacct atatgaacga aaactgcgca cggatcttta aactggataa attgcgaatc
     2401 attgatcaca acatttatga attctttgga cacaacaagg agaatcatta ttttatcaat
     2461 aaaatcaccc aaggacacac caccaccgat gagaatttca cgcttataat aggtaaggaa
     2521 acaattcact gcaacgtcac ctgcaatccg ctgaaaaatg ttgacatgac ggacggaggc
     2581 tccgtggtga ttgtccgcga aagccatcac atcaaccgac tggtgcgcaa ctatatcggc
     2641 ggcggtgcca aagtcacctt tgatgatatt atcgccaaag atcccaaatc cctgcagtat
     2701 gtcaaaaccg ccaaggcagc cgcttcttcc tcatccaatg tattgctgct gggcgagagc
     2761 gggaccggca aggacatcat cgcccaggcc atgcataatg ccagtccccg caaaaacaat
     2821 tccttcctgg ccatcaactg tgccgcactg ccgcgcgatc tgattgccag cgagttgttt
     2881 ggttatgaag acggtgcttt tactggcgcc cgtaaaggcg gcaacatcgg caagtttgag
     2941 ttggctgacc aaggcaccat cttccttgat gaaatcggcg atatgccgct cgatttgcag
     3001 gcatcgctct tgcgcgtgct ggaagaaaag aaagtcatgc ggctgggcgg aggcaagctg
     3061 gttcccgtca atgtgcgcgt gatcgctgcc accaacaaag atttggaaac cgagattgaa
     3121 cgtaaccgct tccggcgtga tttgttctat cgcctgggcg ttatcaaaat aaccatcccg
     3181 ccattacggg aaagaccgga agatatcatg ccgctgacgg aaaatttttt gaaaaaaacc
     3241 tgccgccgct ttaaccggcc gctgatgtcc ttgtcaccgg aggtagagga tgcctttatg
     3301 aactatgatt ggcccggcaa tgtcagggaa atgcagaacg ttattgaagg ttcgacccag
     3361 ttggcacccc atcatgaaat cacgtatgat ctggtcagtc agtatatcac ccccaaagca
     3421 gtcagtctgc cctccgaccc ggggacccaa accgttgcgg tggtagaaaa acaactgatt
     3481 ttggatagtc tggccaagta caagtacaat aaaacggaag tagccaaagc acttggcatg
     3541 tcccgccgta cactgtaccg gcgtttaaac gaatacggga tcatgtaaaa ccgcatcgtt
     3601 ttcagaaatg cataaattga tttactgtat acaaaatgat agtattaatt cagaaaatgt
     3661 gtcttctcat aaaaaacaag aaggcacaat tcggaaaaaa tttattgggg ggtaggtatt
     3721 tttgaagaga gtaaacaaga aatgggcatt cctgctggtt gtcatcatga tcgctgcgtt
     3781 ggccgtcact ggttgtggcc aaaaagccga cgaccagaat cagtcagcgg cacccgaagt
     3841 catcaaggtt ggtgtttatg agccgctcac cggcacaaac gcagccggcg gacaaatgac
     3901 gctcgaagga atcaaattgg ccaaccagct ctatcccgaa gtactgggca aaaaagtgga
     3961 attggtcgtt gttgacaaca agagtgaaaa acaagaagca gccaatgccg tagcccgcct
     4021 ggtagaccaa gacaaagtca acgtgatcat tggcagttat ggcagttccg tttccatggc
     4081 cggcggcccg gtagcccagg atgcccaggt gccggtaatc ggttgttccc ccaccaatcc
     4141 cgcagtcacc ctcggcaacg aatactactt cagagtttgt ttcattgacc ccttccaggg
     4201 aaccgttatg tccaattatg ccagtcagac cctgaatgcc aaaaccgctg ttattattca
     4261 ggacgtacaa caggattatt cagtaggtct gagcaattac tttgaaaaag ccttcaaagc
     4321 caccaatggt gatgcagcca ttctggccaa gctgaactac aataccggcg acaaggattt
     4381 ttccgcgcag ctgaccgaag ccaaatccaa gaatcctgac gttatctttg cccccggcaa
     4441 cttcctggaa tgcggcctga tggtcgccaa agcacgtgaa atgggtatca acgcccccat
     4501 gttgggcgga gacacctggg aagcccagga attcctggat caggttaaag attacaagga
     4561 tatttatttc agtacccatt ttaccgctga acagccggta acagaggaat caacgacatt
     4621 cctgaacgag tacaagaaag cgtatcccga caaggaagtc aatgctttcg ccgccctggg
     4681 ctatgatgcg tacaagctgg ccctggatgc catcaccaga gccgataccg ctgattcaat
     4741 caagatcaga gatgccctgg ctcagaccaa agacttccag ggcgccaccg gca
//
LOCUS       DAOTHZ010000117         4773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_21208_length_4773_cov_8.107885, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000117 DAOTHZ010000000
VERSION     DAOTHZ010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21208_length_4773_cov_8.107885"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1956
                     /locus_tag="P5549_09865"
     CDS             142..1956
                     /locus_tag="P5549_09865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW13417.1"
                     /translation="MEISQSMLCSGVLDALPDAVFAIDRDHRIIAWNAAMERITLVPR
                     AQAIGKAERFYAVPFFGEPCLMLVDLVLNPELEKEYQRPFQKSDMNYYGDGTIKLMSR
                     VNEVFYWSQASPVFDDHGHVIAALEILRDKNSDAVIESKRNLRRITDNMSDLVSELDC
                     HGNVVFASPSHYKVLGYTDQRMMTITLADLVHCEDLPQVTDALKKSLQTGRTGITHHR
                     CRHRDGHYVWTETVGNPIWQDGKVSGIILSSRDITDRKQLEQELRYISTHDALTSLYN
                     RTYFEEELIRLENGRFNPVGMIMFDLDGLKLVNDTMGHEAGDRLLKNFAQILQSCFRS
                     SDIVSRVGGDEFAVLLPESNNQVMDKIAGQVITTIQKYNQINPALPLSVSMGKAIRKD
                     NRSSLRDTFKEADNRMYRTKLQTSGSAHSAVVNTLLKALQARDNLTDGHGERMSRLVD
                     LLGRELKLSESTLTNLQLLARFHDIGKVGIPDRILFKKGKLTEAEYREMQRHSEIGQR
                     IALASPELSSLADLILKHHEYWDGSGYPFGLQGEQIPLECRIIALVDAYDTMTHDRPY
                     SKAVSPQEALEEIARCSGTQFDPKLTKLFLKLLGYSGG"
     gene            complement(2033..2827)
                     /locus_tag="P5549_09870"
     CDS             complement(2033..2827)
                     /locus_tag="P5549_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW13418.1"
                     /translation="MLKICAVSKIFNPGTGMENHALKDINLMVAEGEFVNIIGGNGAG
                     KSTLLNCIAGLHEVDSGSIGLDGMDITFWPEYKRSAYIGRVFQDPLKGTAFDMTIEEN
                     LSMAYAKGKIRGLQRGINKKDQHIFREHLARLDLGLEDRLKQKVGLLSGGQRQALTLL
                     MATIVKPELLLLDEHTAALDPSIARKVLQITREIVTEYKLSTIMVTHNMKAALEYGTR
                     TIMMHEGRIILDLSGEERNAMTVEGLIELFGKKSGTELDNDRLLLN"
     gene            complement(2820..3734)
                     /locus_tag="P5549_09875"
     CDS             complement(2820..3734)
                     /locus_tag="P5549_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW13419.1"
                     /translation="MTFLALQGSIELGIIYALMALGVFISFRTLNMPDLTVDGSFVLG
                     AACSATFCSLGHPLLGILMAILAGWLAGSATALLHTKLKIQPLLAGILMMLALYSVNL
                     RVMGGRANIALVNNTTLFDSFPGQTMEQYAGVILPIVLLLLALWLLYWFLQTRFGLVL
                     RATGDNDQMVRAVGVNTDTTILIGMALSNSLVALSGSLIAQYQSFADISMGVGMVVIG
                     LASVIIGEVLFGIKSLLRRLIAVILGAILYRLVIAIALEMGMPATDLKLISAAIVALA
                     LSAPVIKEKLAAMKRRHAMREGGDRNHA"
     gene            complement(3754..4761)
                     /locus_tag="P5549_09880"
     CDS             complement(3754..4761)
                     /locus_tag="P5549_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW13420.1"
                     /translation="MKKSIVLLGSLLVLLLFSVTGCSSAQKDDATANKESVPQLKIGI
                     VQIVEHPSLNTIRESCVAQLVQNGFKDGENVVIDYQNAQGDQTNLKTICQKFANNQYD
                     MIIAIATPSAQAAVGETSEIPIVFSACTDPIGSGLVDNLEKPGKNVTGTSDAVSAEKI
                     MELAQRITPGIKTIGALYSSSETNSISVVNNLKEYAKKNGMTVVEATVTNSSEAQQAA
                     SSLAGKVDAMFSPIDNTIASAMPIVAQVASKAKIPFYVGADSMVKDGGLATYGINYPV
                     LGQETANMAAEILNGKKPGDIAVKTMTDMDIYLNQATADALGIKIPDDVQKEAAQVFG
                     K"
BASE COUNT         1258 a         1121 c         1169 g         1225 t
ORIGIN      
        1 ggtggaaatc ctgagttttt ttaagacagt ccaaacattg ttgaataaac tttctcaatg
       61 aaggaaaatg atacaaggtt caagaatagt gtaattgaat ttattgggta tcacctaaat
      121 aaacattaca aggagaccga aatggaaatc tcccagtcaa tgctttgctc aggggttctg
      181 gatgctcttc ctgacgcagt ttttgccatc gatcgtgatc atcgtattat tgcctggaat
      241 gcggcgatgg aaagaattac acttgttccg cgtgcgcagg ccattggcaa ggcagagcgc
      301 ttttatgcag tccctttttt tggagaacct tgcctcatgc tggtggatct ggttttaaat
      361 cctgaactgg aaaaagagta tcaacgacca ttccaaaaaa gcgatatgaa ttattatggg
      421 gacgggacca taaaattaat gtcaagggtc aatgaggtct tttattggtc acaggcatct
      481 cctgtttttg atgaccatgg ccatgtgatt gcagcgcttg aaattttacg tgacaagaat
      541 tccgatgcgg ttattgaaag caaacgcaac ttgcgtcgca ttaccgataa catgtcagat
      601 ttggtcagtg aattggattg ccatggcaac gttgtttttg ccagtccatc ccactataag
      661 gtattgggct atacggacca aagaatgatg accattacat tggcagatct ggttcattgc
      721 gaggatcttc cccaggtaac ggatgccctg aagaaatcct tgcaaacggg aagaacaggg
      781 atcacgcatc atcgctgccg gcatcgggac gggcattatg tatggacgga aacggtcggc
      841 aatccaattt ggcaggatgg caaggtgagc ggcattattc tcagcagtcg ggatataacc
      901 gaccgcaaac agctggaaca ggaacttcgt tatataagca cgcatgatgc tttgacttcc
      961 ttgtataacc ggacttattt cgaagaagaa ttaattcgcc tggaaaacgg gcgttttaat
     1021 ccggtgggca tgataatgtt tgacctcgat ggcctcaaat tggtgaacga tacgatgggg
     1081 catgaagcgg gtgaccgttt gttaaaaaac ttcgcccaaa tactgcaaag ctgttttcgc
     1141 agcagtgata ttgtatcccg ggtaggggga gacgaattcg ctgttttact gccagagagc
     1201 aataaccagg tgatggataa aatcgctggt caggttatta ctaccataca gaagtacaat
     1261 caaatcaatc ctgcgctgcc cctgagcgta tccatgggca aggcaatccg caaagacaac
     1321 cgttctagct tgcgggatac gttcaaggaa gctgacaatc gcatgtaccg aaccaaactg
     1381 cagaccagcg gcagtgccca cagtgcagtg gtgaatacgc tcttgaaggc actgcaggcc
     1441 agggacaatt tgaccgacgg tcatggagag cgcatgagca ggctggttga tctattgggg
     1501 agagaattga agctctcgga aagtacgctg accaatctgc aattgttggc acggtttcat
     1561 gatattggca aagtcggtat tcccgatcga attctattta aaaaaggcaa gctgaccgag
     1621 gctgagtaca gggagatgca acgacacagt gaaatcgggc agcgcattgc cctggcttct
     1681 ccggaattaa gttctctggc cgatttaatt ttgaagcatc atgagtattg ggatgggagc
     1741 ggttatccat ttggattgca gggggaacaa attcccttgg aatgtcgcat aattgccctg
     1801 gtggatgcct acgatacgat gacccatgac cggccttaca gcaaagccgt ttctccccag
     1861 gaagcgctgg aggaaattgc tcgttgcagc ggaacccagt ttgatcccaa attaaccaaa
     1921 ctgtttctaa agctgctcgg ttatagtgga ggataaaaag ttttcatgcg aagcgaaccg
     1981 cagacaaaat catctaaacg gactgatggc aatgaaaatt aatgattacc cgttaattta
     2041 gcaataggcg gtcgttatcc agctcggtac cgcttttttt gccgaacaac tcgatcagac
     2101 cttccacggt catggcgttt ctttcctcgc cgcttaaatc caatataatc cggccttcgt
     2161 gcatcatgat ggtacgggtg ccatattcca gtgcggcttt catattatgc gtgaccatga
     2221 ttgtggaaag cttgtattca gtcacgattt cacgggtgat ttgcaatact ttccgggcga
     2281 tggagggatc aagggcagca gtatgttcat ccagcagtaa cagttcgggc ttgacaatgg
     2341 tcgccatcag cagggtcagg gcctggcgct gcccgccgga caataaaccg actttttgtt
     2401 ttaagcgatc ttccaatccc aaatccaggc gtgccaagtg ttcacggaaa atgtgttgat
     2461 cctttttatt gatcccgcgc tgcaatcctc tgatttttcc cttggcgtaa gccatggata
     2521 aattttcttc gatggtcatg tcaaaggcag ttcctttcaa aggatcctgg aaaacccggc
     2581 cgatataagc agaacgcttg tattccggcc aaaaggtaat atccatgcca tctagcccaa
     2641 tgctgcctga atccacctcg tgaagaccgg caatgcaatt gagcagagtt gatttgccgg
     2701 ccccgttgcc gccgattata ttgacaaact caccctcagc gaccattaga ttgatatcct
     2761 tcagggcatg gttttccatg cccgtacccg ggttgaaaat tttactgacc gcgcatattt
     2821 taagcatgat tccggtcgcc tccttcccgc atggcatgac gtcttttcat ggctgccaat
     2881 ttttccttga taaccggtgc cgacaatgcc aaggcgacga tggcagctga gatcagcttt
     2941 aaatccgtgg ccggcatacc catctccaga gcgatggcaa ttaccagacg atagaggatg
     3001 gcccctaaaa taacggcgat taaacgacgc aacagacttt ttataccaaa caatacttca
     3061 ccaatgatga ccgaagccag gccgatgact accatgccca ctcccatgct tatatcagca
     3121 aagctctgat actgggcgat cagcgacccg gaaagagcaa ccagactgtt tgacagggcc
     3181 atgccgatca ggatggtagt atcggtattg acgcccaccg cccggaccat ttgatcgttg
     3241 tcaccggtag cccgcaggac gagaccgaaa cgtgtctgta aaaaccagta gagcaaccac
     3301 aatgccagca ataataatac aattggaaga atgaccccgg cgtattgctc catggtttgt
     3361 ccgggaaaag aatcaaacag cgtggtgttg ttgaccaggg caatattggc cctgccgccc
     3421 atgacccgca ggtttaccga ataaagagcc agcatcatca gaataccggc caacaggggc
     3481 tggatcttca gcttggtatg cagcagggcg gtagccgaac cggccagcca gccggccagg
     3541 attgccatca ggatacccag caacgggtgg cccagactgc aaaaagtagc ggaacaggca
     3601 gctcccagaa cgaagctgcc atccaccgtc aggtctggca tattaagtgt tcggaaagaa
     3661 ataaaaacgc caagtgccat aagggcatag atgattccta attcgattga accctgcaaa
     3721 gccaagaagg tcatttgaaa gactcctaat aagttatttg ccgaatacct gggctgcttc
     3781 cttttgtaca tcgtcaggga tttttatgcc aagtgcatcg gcagtagcct gattgaggta
     3841 gatatccatg tcagtcatgg ttttcacggc aatgtcgcca ggttttttgc cgtttagaat
     3901 ttcagccgcc atattggcgg tttcctgtcc cagaacggga tagtttatcc cataagtagc
     3961 cagtccaccg tcttttacca tggagtctgc acccacataa aaagggattt tggctttgct
     4021 ggcaacctgg gcaacaatcg gcatggccga ggcaatcgtg ttgtctatgg gggagaacat
     4081 tgcatcgact ttccctgcca gcgagctggc ggcctgctgg gcctctgaag aattggttac
     4141 ggttgcttcc accaccgtca tgccgttttt cttcgcatac tcttttaggt tgttaaccac
     4201 cgaaatggaa ttggtttcac tggaactgta taaagccccg atggttttaa tgccgggggt
     4261 gatccgttgg gccagctcca tgattttttc tgcggatacc gcatccgatg taccggtcac
     4321 atttttgccc ggtttttcaa gattatcaac cagtcctgaa ccgatgggat cagtacaagc
     4381 agaaaatacg atcggaattt cgctggtttc tcccaccgca gcctgtgctg agggggtggc
     4441 aatggcaatg atcatatcat attgattatt ggcaaacttt tggcagatgg ttttcaaatt
     4501 ggtttgatca ccctgcgcat tctggtaatc aatcaccacg ttttccccgt ctttgaaacc
     4561 gttttgcacc aattgtgcca cgcatgattc cctgatggtg ttgagagagg gatgttcaac
     4621 gatttgcacg atgcctattt tcaactgcgg gacactttct ttgttggcag tggcatcatc
     4681 cttttgggca gacgaacaac ctgtcactga aaataaaagc agtaccaata atgaacccaa
     4741 caacacaata gactttttca tttcgtatcc tcc
//
LOCUS       DAOTHZ010000118         4769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_21232_length_4769_cov_10.485575, whole genome shotgun
            sequence.
ACCESSION   DAOTHZ010000118 DAOTHZ010000000
VERSION     DAOTHZ010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21232_length_4769_cov_10.485575"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..432)
                     /locus_tag="P5549_09885"
     CDS             complement(70..432)
                     /locus_tag="P5549_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRW13421.1"
                     /translation="MSKVYCQVYRTENHRLIADQVEVACSFFSRLRGLLGRTKMTAGS
                     GLILQPCSSIHCFGMRFPIDAIFLDRQHRVITIKENMPPGSWASQELAYSVLELCAGE
                     ARKHQISVGEQLKLFYLS"
     gene            complement(459..1382)
                     /locus_tag="P5549_09890"
     CDS             complement(459..1382)
                     /locus_tag="P5549_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRW13422.1"
                     /translation="MLFVTITVFACIFCLSLAFMDRWLAPVLDKKRRLQDIKKQTAVQ
                     VQGGFQEELQMNINERIFKPLLAKISTLTQKYTPVKQTGFIEQKLDYAGHPFGWKASD
                     FIAAQYASAFGLGILAGASSLFLFYSSLSNAIMALLLGFIFGYLIIDIFLKRTISSRQ
                     EAIRHELPDVLDLLTISMEAGLGFDASMQRVAQRSSGPLAAEFHQTLQEMRMGKTRRD
                     ALKDLGERNGVDDLSKFTDAIIQADKLGVGLGNVMRGQADQMRVLRRQRVEEKAMKAP
                     VKMLLPLVLFIFPTIFVVILAPAIIQMLDTF"
     gene            complement(1395..2357)
                     /locus_tag="P5549_09895"
     CDS             complement(1395..2357)
                     /locus_tag="P5549_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRW13423.1"
                     /translation="METIISAMIFLAASGFVIGLHRLSGEKKEQLNKRLDSINQSLKD
                     ETADQTEGKSRFDMKALFSAMGKSFVSLSFTKNIEGELAKADVLMRSEEFIGLNIVTT
                     ILGGFAGTLLLGFGSAPVIFALLGAFAPWLFLMWKKQQRAAILNSEIADSLTGMSNSL
                     RTGYSFQQAMDMVSKETAGPLATEYRRTLREINLGVTTEQALHNLIQRVGDDDLELMI
                     GAVLIQRQIGGNLAEIFDNIADTIKQRIRMRGEVRALTAQGRVSGIIIGALPIGLLLI
                     LMAINPDYMGVLFASKTGWYILGGAAVSETIGFIAIKKITDIKL"
     gene            complement(2371..3741)
                     /locus_tag="P5549_09900"
     CDS             complement(2371..3741)
                     /locus_tag="P5549_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRW13424.1"
                     /translation="MSLAQRLQGSQAAVMKDNFPSSLQENSIAEEKSDKYAELKARIH
                     LEVIDKIEEDMKQDDLTNEMLKKKTREIIFRLLERDAVNLPFSERQMIAAELMDDVLG
                     LGPIENLLKDNEVTEVMVNGPKQVYVERGGKLYLTEVKFRDDEHVMHIIDKIVSPIGR
                     RIDEGQPMVDARLPDGSRVNAIIPPLALKGPSITIRKFSNNPYKIHDLIRFGTLTPKM
                     AKLLEAAVKARLNIIISGGTGSGKTTLLNVLTGYIPENERIVTVEDAAELKLSQDHVV
                     SLESRAENIEGKGAVTIRDLVRNCLRMRPDRIVVGEVRGGEALDMLQAMNTGHDGSLT
                     TGHANSPRDMLARLETMVLMAGMELPVKAIRQQVSSAIDLVVQQSRLRDGSRVITHIT
                     ELLGMEGDIIQLQDIFVFEQKGVDKDGKVVGSHHATGITPKVLARIEAEGVHLDKEIF
                     HPDRND"
     gene            complement(3753..>4769)
                     /locus_tag="P5549_09905"
     CDS             complement(3753..>4769)
                     /locus_tag="P5549_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW13425.1"
                     /translation="AHPNIGVILMDEREDSNTVKQAMMAGARDFIPWENLKAEELSQS
                     VRRLYQESKARAASLHTIKSDAREQNVKSPQVVTIYGAKGGTGKTTLTVNMAVAIARA
                     AKRRVVIIDLNLQFGDVAVYMDAKPRRTIAELVQERSQWDGPLVNSYLIEHSSGVKIL
                     PAPLRPEDAELVSADHVEKIIAILRESYDFIIIDSSPYMSDALLSALDTSNQIIFLMT
                     MDLPAVKNAKLGLRLLTGLNHSGKTKLVINRGSKQFGIDLKDIEKTIDFLAAAEIPSE
                     GNTVVNAANKGIPFIISSPQSAVSKAVENVARMVVEDTGYQSDLKVKQERGSQGIFKK
                     KLFR"
BASE COUNT         1008 a         1280 c         1244 g         1237 t
ORIGIN      
        1 atgatgaata tgagatcttc ctgctttaat gattccgtgt aaactcaatt ttgccggaga
       61 tgttccctgc tacgacagat aaaacagctt taattgttct ccgacggaga tctgatgctt
      121 gcgggcttcc ccggcgcaca gttccaggac ggagtaagcc agttcctggg aggcccagga
      181 gccgggaggc atgttttcct tgatggtgat cacccggtgc tggcgatcca aaaagatggc
      241 atcaatggga aatctcatgc cgaaacagtg gatggaggaa cagggctgta aaatcaggcc
      301 ggaaccggcg gtcatttttg tccgtcccag gagaccgcgc agccgggaga agaaagaaca
      361 ggccacttcc acctggtcgg cgatcaaccg gtgattctct gttcggtaaa cctggcaata
      421 gactttgctc atttttcatg cctcctttcc tgccgatatt aaaaggtatc cagcatctga
      481 atgatggccg gagctaaaat aacgacaaag atggtgggga atataaacaa taccagaggc
      541 agcagcattt ttaccggggc tttcatggct ttttcctcta cccgctgacg gcgcagcacc
      601 cgcatctggt cggcctggcc gcgcatgacg ttgccgaggc cgacgcccag tttgtcagcc
      661 tggatgatgg catcggtgaa tttgctcaga tcatcaacgc catttcgttc tcccaggtct
      721 tttaatgcat cccgccgggt cttgcccatg cgcatttcct gcagggtctg atggaattcc
      781 gctgccagcg ggccggagga tctctgcgcc acccgctgca tggatgcgtc aaaacccagg
      841 ccggcttcca tgctgatggt aagcaggtcg aggacatcgg gcagctcatg tctgatagcc
      901 tcctgccggc tgctgatggt gcgtttaaga aatatatcga tgattaaata gccgaaaata
      961 aatcccagta acagcgccat tattgcattg gaaaggctgc tgtaaaacag gaacaaactt
     1021 gacgctccgg ccaggatccc caggccgaat gcggaggcgt attgagcggc aataaagtcg
     1081 gaagctttcc acccgaaggg gtgtccggcg taatcaagct tttgttcgat aaaaccggtt
     1141 tgtttgaccg gggtatattt ctgggtcagg gtcgatattt ttgccagcag gggcttgaag
     1201 atccgctcat tgatgttcat ctgcagttct tcctggaacc cgccctggac ctggactgcg
     1261 gtctgctttt tgatatcctg caggcgccgt ttcttgtcca ataccggtgc cagccagcgg
     1321 tccatgaatg ccagggacaa acagaatata caggcaaata cggttatggt gacgaacagc
     1381 atctcttctc ctccttacag cttgatatca gtgatttttt tgatggctat aaatcctatt
     1441 gtctcggaaa cggcagctcc gcccaggata taccatccgg ttttgctggc aaaaagtaca
     1501 cccatataat ccggattgat cgccatcagg atcaacagta aaccgatggg caaggctcct
     1561 attattattc cggagactct tccctgggca gtcagagccc tcacttcgcc ccgcatgcgg
     1621 atgcgctgct tgatggtatc ggcaatgttg tcaaatatct cagccaggtt gccgccgatc
     1681 tgccgctgga tcaggaccgc cccgatcatc agttccagat catcatctcc cacccgctgg
     1741 atcaggttgt gcaaggcctg ctcggtggtg acccccaggt tgatctcccg cagggtacgg
     1801 cggtattccg tggccagggg accggcggtc tccttgctga ccatatccat ggcctgctga
     1861 aaactgtatc cggtcctaag ggaattggac ataccggtga gggaatcagc gatctcgctg
     1921 tttaaaatgg cagcgcgctg ctgcttcttc cacattaaaa acaaccaggg ggcaaacgct
     1981 cccagcaagg caaaaatgac tggcgctgag ccgaatccca gcagcagggt gccggcgaat
     2041 ccgcctaaaa tggtggtaac aatattgagg ccgataaatt cttcactgcg catcaacacg
     2101 tcggctttgg ccagttcccc ttcaatattt ttggtgaagg acagggaaac aaagcttttg
     2161 cccatggcac taaacagtgc tttcatatcg aacctggact tgccttctgt ttgatccgcc
     2221 gtttcatcct ttaaggattg gttgatgctg tccagccgct tgttgagctg ttcctttttc
     2281 tccccgctca aacggtgcag accgataacg aagccgctgg cggccagaaa tatcattgcg
     2341 ctgatgatgg tttccataaa tcatatcccc ttaatcattt ctgtcgggat gaaatatttc
     2401 cttatccagg tgaacgcctt ccgcctcgat ccgggcaagg actttgggcg tgatgccggt
     2461 ggcatggtgg ctgcccacca ctttgccgtc tttgtccaca cctttctgtt caaaaacaaa
     2521 gatatcctgc agttggatga tatcgccttc cattcccagc agttcagtaa tatgggtgat
     2581 gacacggctg ccgtcccgca gacggctctg ctgcaccacc aggtcgatgg cggatgacac
     2641 ctgctggcgg atggctttga ccggcagttc cataccggcc atcagcacca tggtttccag
     2701 acgggccagc atatctctgg gtgagttggc gtggccggtg gtcagggaac cgtcatgccc
     2761 ggtattcatg gcctgcagca tatccagggc ttccccgccc ctgacctcgc cgactacgat
     2821 gcggtcaggg cgcatacgca ggcagttgcg caccagatca cgaatggtga ctgcgccttt
     2881 gccctctata ttctcggctc tggattccag actcaccaca tggtcctggc tcagtttcag
     2941 ttccgccgcg tcttccacgg taacgatgcg ctcgttttcc gggatatagc cggtcagaac
     3001 attgagcagg gtagttttcc cgctgccggt gccgccggaa atgatgatat tcagccgggc
     3061 cttaacggcg gcttccagta atttcgccat tttcggagtc agggtgccga agcggatcag
     3121 atcatggatc ttatatggat tgttggaaaa cttacgaatg gtgatactgg gcccctttaa
     3181 ggccaggggc ggaataatgg cattgacacg ggagccgtca ggcagacggg catccaccat
     3241 gggctggcct tcatctatcc tccggccgat gggcgataca attttatcga tgatgtgcat
     3301 gacatgttcg tcgtcgcgga actttacctc ggtcaggtag agcttgccgc cgcgttccac
     3361 atatacctgt ttgggtccgt tgaccatgac ttctgtcact tcattgtcct tgagcaggtt
     3421 ttcaatgggt cccagaccca aaacatcatc catcaattca gcggcaatca tctggcgttc
     3481 cgaaaagggc agattgaccg catctctttc cagcaggcgg aaaatgatct ccctggtttt
     3541 ctttttcagc atctcattgg tcaggtcatc ctgtttcatg tcctcttcaa ttttatcgat
     3601 gacttccaga tggatcctgg cctttaattc agcatatttg tcactctttt cttctgcgat
     3661 gctgttctcc tgcaggcttg aagggaaatt gtctttcatt accgctgcct ggctgccctg
     3721 cagtctttgt gccagtgaca tatgcttcac tcctatctaa acagcttttt tttgaatatt
     3781 ccctggctgc cccgctcctg cttcaccttc agatcgctct ggtatccggt gtcttctacc
     3841 accatccggg cgacattttc caccgctttg ctgacggcgg actgggggct gctgatgata
     3901 aagggtatgc ccttgttggc ggcattgacc acggtgttgc cttcactggg tatttcagcc
     3961 gctgccagaa agtcgatggt cttttcgata tcttttaagt cgatgccgaa ctgttttgag
     4021 ccgcggttga taaccagctt ggttttgccg ctgtggttta atcctgtcag taacctcagg
     4081 cccagtttgg catttttcac cgccggcagg tccatggtca tcaggaagat gatctgattg
     4141 gaggtgtcca gcgctgacag gagggcatca gacatatagg gcgaagaatc aataatgata
     4201 aaatcgtaag attcacgcag gatggcaata atcttttcca cgtgatcagc gctgaccaat
     4261 tcagcatctt ccgggcgcag cggggcaggc agtattttca ccccggaaga atgttcgatc
     4321 aggtagctgt tcaccagcgg gccgtcccat tggctgcgtt cctggaccag ctcggcaatg
     4381 gtccgccgtg gtttggcatc catataaacg gctacatcgc cgaactgcag attgaggtcg
     4441 atgatcacca cccggcgttt ggctgcccgg gctatggcaa cggccatatt gacggtcagg
     4501 gtagtctttc cggtcccgcc tttggctccg tatatggtga ctacctgggg tgatttgacg
     4561 ttttgttccc tggcatcgga ctttatggta tgcagactgg cggcgcgggc tttggattcc
     4621 tgatataaac gccgcactga ctgtgacagt tcctctgcct tcaggttttc ccaggggata
     4681 aaatcccggg ctccggccat catagcctgt tttaccgtat tgctgtcttc ccgctcatcc
     4741 atcaggatca cacctatgtt gggatgggc
//
LOCUS       DAOTHZ010000119         4767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_21246_length_4767_cov_6.550509, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000119 DAOTHZ010000000
VERSION     DAOTHZ010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21246_length_4767_cov_6.550509"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="P5549_09910"
     CDS             <1..1436
                     /locus_tag="P5549_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRW13426.1"
                     /translation="LVSGYDGGTGAAPRTSIRNAGLPWELGLAETHQTLVLNRLRDRV
                     TLETDGKLMSGRDVVIAAMLGAEEFGFATTPLIALGCVMMRVCNLNTCPVGIATQDEK
                     LRKNFVGQPEHVEHFMRFVAQEMREIMARLGFRTVKEMVGRTDKLKNKDVKHWKASHL
                     DLSPILYQPYAGSDVGRRKMLPQNHLLEKSLDARKLLRMCQPALEKGKPIRAKLKINN
                     VDRVAGTMTGSEITKKFGENGLPDDTIKLTFVGSAGQSFGAFTPRGMTLELEGDANDY
                     IGKGLSGSKIIVYPPRGAGLEPDQNILVGNVAFYGATAGEAYISGIAGERFCVRNSGI
                     QAVVEGVGDHGCEYMTGGKVVILGHTGRNFAAGMSGGIAYVLDFDEARCNKAMVLLEP
                     LEAAEEIDEVKAMIRHHVGYTASPLGKKVLEDWENYLPRFTKIIPTVYKQIMQEIARN
                     IAKPKRKTTVKSATGWQTDRQEWNQGG"
     gene            1439..2923
                     /locus_tag="P5549_09915"
     CDS             1439..2923
                     /locus_tag="P5549_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRW13427.1"
                     /translation="MGKATGFLEYSRVESAQRAPEERIKDWHDIRLPQDQKALQRQAA
                     RCMNCGIPFCHSGILLNGMTSGCPLHNLIPEWNDLVYKGRWKEAYQRLTRTNPFPEFT
                     GRVCPAPCEGACTVGYLWDPVTIKNIEYEIIEMAFAQGWVKPVKGTPTGCKVAVVGSG
                     PAGLAAAHYLNAVGHDVTVFERDDRPGGLLVYGIPNMKLDKKIIARRIEVMQAAGIKL
                     VLNTEIGTDLPAADLTGNFDAVLLCAGAGKPRHLQVEGHDLKGVYLALDFLKGSTRSM
                     LDENKNDSLFINARDKNVIVIGGGDTGTDCAATAIRQKCQSVYQFEIQPEPPDRRIKP
                     SNPWPEWPRKQKVDYGQAEAISISGEDPRHYLLATKKIEGDKHGRVRAVHTVGVNWFK
                     NASGCLEPMEVPGSEKVWPADLVLLALGFVGTEDKLPEEMRLSRDGHGNVQAEFGVFE
                     TSAEKVFAAGDMRRGQSLIVWAIQEGKLAARSVDRYLTGDSLIK"
     gene            3000..3320
                     /locus_tag="P5549_09920"
     CDS             3000..3320
                     /locus_tag="P5549_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13428.1"
                     /translation="MKERNGKNAPNEHFEFGKQCTYCRAQRSHEYELCKYCGVYRQGL
                     KNMAELWPGNIKPSIGELMARFDFASREMIKLYCQSGLDRKQIETIMDKIVRDNIRSL
                     AMKK"
     gene            3414..4505
                     /locus_tag="P5549_09925"
     CDS             3414..4505
                     /locus_tag="P5549_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRW13429.1"
                     /translation="MRPNRFVVLENGQVFSGKPFGAEGDILGEIVFTTGMTGYLETLT
                     DPSYYGQIVIQTFPLIGNYGVIPADFENSSPKVKAYIVREWCQVPSNFRSEGELDAFL
                     KRHNIPGVYGIDTRELTKVIREVGVMNAKITSEIDDLDKILAEIRSYRIENAVENVSS
                     SEISIEQGEPGGFKVVLWDFGAKENIRRELLKRGCEVITVPSHTTAQAIAGFKPDGIM
                     LSNGPGDPADNTEIISELARLTETGLPIYGICLGHQLLALAQGGQTAKLKYGHRGANQ
                     PVKDLNQGKVYITSQNHGYAVVAESLPSHAVMRFVNVNDGTCEGIEYKNMPAFSTQFH
                     PEASAGPLDTCYLFDQFMDLMREEKAKCR"
     gene            4496..>4767
                     /locus_tag="P5549_09930"
     CDS             4496..>4767
                     /locus_tag="P5549_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13430.1"
                     /translation="MPLNQNIKKVLVIGSGPIVIGQAAEFDYAGTQACRALKEEGLEV
                     VLVNSNPATIMTDYAMADQIYIEPMTLETIKRIIIKERPDSVLSTLG"
BASE COUNT         1276 a         1111 c         1347 g         1033 t
ORIGIN      
        1 tcctggtcag cggctacgac ggcggaaccg gtgctgcacc ccgcaccagc atcaggaatg
       61 ccggcctccc ctgggaactt ggtctggcag agacccacca aaccttggtg cttaatcgct
      121 tacgggatcg ggttaccctg gagaccgacg gcaagctgat gtctggtcgt gatgtggtca
      181 ttgccgccat gctgggcgcg gaggaatttg gctttgccac cactccgctg attgcgctgg
      241 gctgtgtgat gatgcgggtt tgcaacctca atacctgccc ggtcgggatt gctacccagg
      301 acgagaagct gcgcaaaaat ttcgtcggtc agcccgaaca cgtggagcac tttatgcgct
      361 ttgtggccca ggaaatgcgg gagattatgg cacggctagg tttcaggacc gtaaaagaga
      421 tggtcggccg caccgacaag ctgaaaaata aagacgttaa acattggaag gcttcccatt
      481 tggatctttc cccgatcctc taccagccgt atgccgggtc agatgtaggc cgcagaaaaa
      541 tgctgcccca gaaccatttg ctggagaaat cactggatgc ccgcaagctg ctgcggatgt
      601 gccagccggc cctggagaaa gggaagccga tccgggcgaa gctgaagata aacaatgtgg
      661 accgggtggc aggtaccatg accggcagtg agatcaccaa aaagttcggg gaaaacggat
      721 tgcccgatga taccatcaag cttacctttg tgggttccgc cggccagagt ttcggcgctt
      781 ttaccccccg gggcatgacg ctggaactgg agggagacgc caatgattat atcggcaagg
      841 gtttgtccgg tagcaagatc atcgtttatc ctcctcgggg agcaggcttg gaaccggatc
      901 aaaatatcct ggtcggcaat gtcgctttct atggcgcgac tgctggagaa gcctatatca
      961 gcgggattgc cggggaaagg ttctgcgtta gaaacagcgg catccaggcg gtagtggaag
     1021 gcgttggcga ccatggctgc gaatacatga ccggcgggaa agtcgtgata cttggccata
     1081 ccgggcgtaa ttttgccgca ggcatgtcag gcgggattgc ttatgtgctt gattttgacg
     1141 aggcgcgctg caacaaagcc atggtactcc ttgagccgct tgaagctgcg gaagaaattg
     1201 atgaggttaa agcgatgatc cgccatcatg ttggctatac cgcaagtccg ctggggaaga
     1261 aggtgcttga agattgggag aattatctgc ctcgctttac caaaataata ccgaccgtat
     1321 ataaacagat catgcaggag atagcccgga atattgccaa accaaaaagg aaaacgacgg
     1381 tcaaatcggc cacaggttgg cagacagacc gccaggaatg gaaccagggg gggtagtgat
     1441 gggaaaagca accggttttt tagaatactc cagagttgaa tcagcccaac gggctcctga
     1501 agaacggatt aaagattggc atgatatcag gctgccgcag gatcagaaag ccctacaaag
     1561 gcaagcagcc agatgcatga attgcggcat tccattctgt cacagcggta tcctgttaaa
     1621 cgggatgact tccggatgtc cgctgcataa tttgatccct gaatggaatg atttggttta
     1681 caaaggacga tggaaggaag cttaccagag actgacccgc accaacccgt tcccggaatt
     1741 taccggccgg gtatgtcctg caccctgtga aggagcctgt acggtcgggt atctctggga
     1801 tccggtgacg ataaaaaata ttgaatacga aatcatcgag atggcatttg cgcaaggttg
     1861 ggtcaaaccg gtaaagggaa ctcccaccgg ttgcaaagtg gcggtggtag gttcgggtcc
     1921 ggccgggttg gctgcggcac attatttgaa tgcggtcggc catgatgtaa ccgtttttga
     1981 acgtgacgac cgtcccggcg gtttattggt ttatggaatc cccaacatga agctggacaa
     2041 gaaaattatt gccagacgaa tcgaagtgat gcaggcggcc gggatcaaat tggtgctcaa
     2101 tacggaaatc ggcaccgatc tgccggctgc cgatctgact ggaaatttcg atgcggtcct
     2161 tttgtgtgcc ggtgcaggca agccccgaca tttacaggtg gaggggcatg atctcaaggg
     2221 cgtttatttg gcgcttgatt ttctcaaagg cagtacccgc agcatgttgg atgaaaataa
     2281 aaatgacagc ctctttatca atgcccgcga caaaaacgtg atcgtgatcg gcggcggaga
     2341 taccgggaca gactgtgcag ccaccgccat ccgtcagaaa tgccaaagtg tttatcagtt
     2401 tgaaatccag ccggaacccc cggaccggcg gatcaaacca tccaatccct ggccggaatg
     2461 gccgcgcaaa caaaaagtgg actacggcca ggcagaggcc atcagcatca gcggcgagga
     2521 tccccggcat tatctgctcg ccaccaaaaa gattgagggc gacaagcacg gccgggtacg
     2581 ggcagtgcat accgtaggag tcaattggtt taaaaatgct tccggatgtt tggagcccat
     2641 ggaagtaccc ggcagtgaaa aagtatggcc tgccgatctg gtattgctgg ctctgggttt
     2701 tgtcggaact gaagataaac ttccggaaga gatgagactg agccgggatg ggcatggcaa
     2761 cgtccaggct gagtttggag tattcgaaac cagtgcagaa aaagtatttg ccgccggcga
     2821 tatgcgcagg ggacaaagct tgatcgtctg ggcgatccag gaaggcaaat tggctgccag
     2881 aagcgtggat agatatctga ccggagacag cttgatcaaa tagctgacca tgtcggaaga
     2941 tagatcaata gcggccggta aaaatggtat ctctgaagaa atagatttga gggataataa
     3001 tgaaagagcg caacggaaaa aatgccccga atgaacactt tgaatttggt aaacagtgta
     3061 catattgccg ggcgcaaaga agccatgaat acgaattatg caagtattgc ggggtttaca
     3121 gacaagggct gaaaaatatg gctgagctgt ggccgggcaa cataaaacca agtataggtg
     3181 aattaatggc caggtttgat tttgccagcc gcgaaatgat aaagctatac tgccagtcag
     3241 gcctggaccg caagcaaatt gaaaccatta tggataaaat tgttcgggat aatattaggt
     3301 ccctggccat gaagaaatag tattatcata aaccatcatt ataatgtggc agcttgaaaa
     3361 gaaatggcag tgatccttcc gccatgctgt cataaggaaa aggagcgttc aggatgagac
     3421 caaaccgctt tgttgttctg gaaaacgggc aggttttcag cggtaaacca tttggggcag
     3481 aaggagatat cctcggtgaa atcgttttta ccaccggcat gaccggttat ctggaaacat
     3541 tgaccgaccc cagctattac ggtcagatcg tcattcaaac ctttcccttg attggtaact
     3601 acggagtgat ccccgccgac tttgaaaatt cttcccctaa agttaaggcc tatatagttc
     3661 gtgagtggtg tcaggttcca tccaattttc gcagtgaggg tgagcttgac gcttttttaa
     3721 agcggcataa tattcccggt gtctatggca tcgatacccg ggaactgacc aaagtgatca
     3781 gagaagttgg ggtcatgaat gccaaaatca cctcggaaat cgatgatctc gataaaatac
     3841 tggcagaaat ccgcagttac cggatcgaaa atgctgtgga gaacgtcagc agcagcgaga
     3901 tatcgataga acaaggagaa ccgggcggtt ttaaagtggt gctgtgggat ttcggagcca
     3961 aggaaaatat ccgccgcgag ttgctgaaac gcggctgtga agtgattaca gtaccttccc
     4021 atacaacggc ccaagccata gcgggtttta aaccggacgg gatcatgctt tccaacggac
     4081 cgggcgatcc agccgacaat acagagatta tcagcgaact ggccagattg actgaaaccg
     4141 gcctgccgat ctatgggatc tgcctggggc atcagctgct ggccttagcc cagggagggc
     4201 aaaccgccaa actcaaatac ggtcatcgcg gagccaacca gccggtcaag gatttgaacc
     4261 aaggcaaagt atatattacc agccaaaacc acgggtatgc agttgtggct gaaagtcttc
     4321 cttcccacgc ggtcatgcgc tttgtcaatg tcaatgacgg tacttgcgaa gggatcgagt
     4381 ataaaaacat gccggctttt tcgacccagt ttcacccgga agcttcagca gggcctctgg
     4441 atacctgcta tttatttgac cagtttatgg atctcatgcg ggaggagaaa gcaaaatgcc
     4501 gctgaaccag aatataaaaa aagtgcttgt gattggctcg ggaccgattg tcatcggcca
     4561 ggccgccgaa tttgactatg cagggacgca agcctgccgg gccctgaaag aagagggact
     4621 ggaggtcgtc ctggtcaact ccaatccggc tactattatg accgattatg ccatggccga
     4681 tcaaatctat atcgagccga tgacactgga aaccatcaag cggatcatca tcaaggaacg
     4741 ccctgacagc gttttatcaa ccctggg
//
LOCUS       DAOTHZ010000120         4680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_21775_length_4680_cov_6.779459, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000120 DAOTHZ010000000
VERSION     DAOTHZ010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21775_length_4680_cov_6.779459"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="P5549_09935"
     CDS             complement(<1..516)
                     /locus_tag="P5549_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRW13431.1"
                     /translation="MLQLEQVNKRFLHNGEAVQVLQDISFTVPPGDFFCLLGPSGCGK
                     TTLLRCIGGFEPISSGSIKLNNHSITAPGIDRIMVFQGFDQLFAWKTVAANIEYPLKI
                     NGLDRAQRKDRVRQYLSLVGLSDYARYYPHQLSGGMKQRTAIARALALEPRLLLMDEP
                     FGNLDALTRHNM"
     gene            complement(531..1325)
                     /locus_tag="P5549_09940"
     CDS             complement(531..1325)
                     /locus_tag="P5549_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRW13432.1"
                     /translation="MPVRMNKLLQNNLFQKSLIVILLILIWEMTARLGWVNQLLLPGF
                     TTVGETFADQLRNGNLYIMTMFSLLMIVKGMLISLLMALVLSSLAMMFHWAENLVNVL
                     NSIFHPLPGIALLPIAMLWFGLSEASIIFIIVHSVVWPLVINFLSGFRSIPPTIMEAG
                     RNIGLQRAGLIWGIMLPAAAPYLISGIKIGWARAWQAAIAGEMVFGAVGTIGGLGWYI
                     FKTRYDFHIAGTFAALLTIMVVGILIEEVLFKSLEKATVKKWGMSI"
     gene            complement(1306..2370)
                     /locus_tag="P5549_09945"
     CDS             complement(1306..2370)
                     /locus_tag="P5549_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRW13433.1"
                     /translation="MRMRNRGKYYLVLVLILSLMIAGCSSGKNEPDGSYAPQQKVTIA
                     IQYGLAYAPLQIVQQQKLVEKYLPGTEVIWKQLGTGPVIRDAMVAGEVDIGFMGISPF
                     LVGWDKGAQWKICTASGSQPVILVTNKQNIGSIKDFTVHDKIATPALASIQHILLAMQ
                     AEKELGNAHALDNNLLSVTHPDAMAGLLSKKDVTAHFSSPPYLYKELQDSAITEVLDG
                     EDAFGQEFTFIFGVATEKFHDANPSTYNAFIGAFNEAVAFINNHPQEAAAILAPQYQY
                     SEEETWRYLTWEGTNYCTTPYGIMGVAEFMHQQGFISKMPQSLEEIAFENVQAAIGSR
                     YGEKSVIETLQERQNAGKNE"
     gene            complement(2384..3667)
                     /locus_tag="P5549_09950"
     CDS             complement(2384..3667)
                     /locus_tag="P5549_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRW13434.1"
                     /translation="MKLLCLGAGFNQLNAIRKAKSKGYTLVVSDYLPDAPGKRWADHA
                     ELVSTFDVEANIEVARKYGVDGVFAIGTDQPVYTAACVAQALGLAHMITPWTALRATN
                     KKYMKECFAANHIPCSRHCFIHKDELQDWEHLRKKLSPLRFPAVVKPLDSQGQRGIFK
                     LPALQAGMANYMRHTFAYTRSDEIIVEEFFAGDEITVSAWVDEGQAKTLMVTDRPLIN
                     IEPHLGTPDGHVYPSPYWTSHHTAITELVNKICRSFDIQNGPLYIQMMSNGGEFQVVE
                     IACRIGGGHEEELIPLVTGIDVVDLFLDRSAGKAISLTNFDPVDPSAIRKNAMVKFIV
                     ARPGMVDHCGDLEKIKTLPGVVNAGFYHPEMERVEELVDSTKRIGYLLMEGKDPMDLQ
                     RKTRFAYQHLQVLNPQGENLVVDTWDAHSIYRVYR"
     gene            complement(3669..4409)
                     /locus_tag="P5549_09955"
     CDS             complement(3669..4409)
                     /locus_tag="P5549_09955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRW13435.1"
                     /translation="MPSISVVIPVYNSEHSLAELYARLVRVLESLQCDFEIILVDDCS
                     QDNSLEIIRSLRKQDPRVEMIQLACNHGQHLATLCGMQYSRGALIITMDDDLQQLPEE
                     IPLLLAKIESGGYDVVFAVPARKKQAFYRNLGSHLMQSSLTLLFSKPRDIESSSYRVL
                     TRPLAESMLNNQPRFVYFAALIFQYTNNSGNVTVKHEKRKYGSSNYTLASALRLALNL
                     YLNYAFAKPQSSRPFHFDIACLEVNGGN"
     gene            complement(4427..>4680)
                     /locus_tag="P5549_09960"
     CDS             complement(4427..>4680)
                     /locus_tag="P5549_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRW13436.1"
                     /translation="LCPTQAKRDELLQSLREQGVEASFHFIPLHSSPMSRRLGYGKDE
                     LPVTEEISRRLIRLPIYPDLSEDEQAYVIEKVQECLTGW"
BASE COUNT         1166 a         1227 c         1104 g         1183 t
ORIGIN      
        1 catattgtgg cgggtcaagg catccagatt gccaaaaggt tcatccatta gcaacaaacg
       61 tggttccaag gccagcgctc tggcaatggc tgtgcgctgt ttcattcccc cggacagctg
      121 atggggataa tagcgggcat aatcactcag accaaccagg gaaaggtatt gtctgacccg
      181 gtccttgcgc tgcgcccgat ccaatccgtt gattttgagc gggtattcaa tattggctgc
      241 tacggtcttc caggcaaaaa gctgatcaaa accttggaag accatgatac gatcgatccc
      301 gggcgcagtt atgctgtgat tgttcagttt gatcgaaccg ctgctgatcg gctcaaaacc
      361 gccaatacag cgcagcagcg tagtcttgcc gcagccggat ggtcccaata gacagaaaaa
      421 atcaccgggc ggcaccgtaa aactgatgtc ctgcaaaacc tgaaccgctt ccccattatg
      481 cagaaatctt ttgtttacct gctccaactg caacatttgc cggcaacctc ctaaatactc
      541 atgccccatt tttttacggt ggctttttcc aggcttttaa acaggacttc ctcaatcaaa
      601 atgcccacca ccatgatggt cagcaaggca gcaaaagtac cggcaatatg aaagtcatac
      661 cgggttttga aaatatacca gcccaatcct ccgatcgtgc ctaccgctcc gaacaccatc
      721 tcgccggcaa tggccgcttg ccaggcccgg gcccagccaa tttttatgcc ggaaatcaag
      781 taaggagcag cggccggcag cataatcccc cagatcaacc cggccctttg cagcccgatg
      841 ttgcggccgg cttccataat cgtcggcgga atggagcgga aaccgctcaa aaaattaatt
      901 accagcggcc aaaccacgga atgaacgata atgaagatga tgctggcttc gctgagtcca
      961 aaccacagca tggcaatggg cagcagggcg atgccgggca gcggatgaaa gatggagttg
     1021 agcacattga ccaggttttc tgcccaatga aacatcatcg ccagggagct caaaaccagc
     1081 gccatgagca gggatatcag catgcctttc acgatcatca agagagaaaa catggtcatg
     1141 atatacagat tgccattgcg cagctgatct gcaaacgttt cgccgacagt ggtgaagccc
     1201 ggcagtaaaa gctgattgac ccaacccagg cgggcagtca tttcccagat cagaattagc
     1261 aggatcacga tcaggctttt ctgaaataaa ttattttgca aaagtttatt cattcttacc
     1321 ggcattttgt ctctcctgca gcgtctcaat cacggatttc tcaccgtagc ggctgccgat
     1381 ggcggcctgt acgttttcaa aagcaatttc ctccagggat tgaggcatct tgctgatgaa
     1441 tccctgctgg tgcatgaatt cggccacgcc catgatcccg taaggggtgg tacaataatt
     1501 ggtgccctcc caggttagat agcgccaagt ctcttcctca ctgtactgat attgcggggc
     1561 caaaatggct gccgcctctt ggggatgatt gtttataaat gccaccgctt cattaaaggc
     1621 tccaataaag gcgttgtagg tgctgggatt ggcatcgtgg aatttttcgg tagctacccc
     1681 aaagataaag gtgaattcct ggccaaacgc atcttccccg tccagcactt ccgtaatggc
     1741 agaatcctgc agttctttgt acaaataagg aggagaagag aaatgggcgg tgacatcttt
     1801 tttgctcagc aagcccgcca tggcgtcagg atgggtgaca gacaacagat tattgtcgag
     1861 ggcatgggca ttgcccaatt ccttttcggc ttgcatggcc agtaaaatgt gctggatact
     1921 ggccagcgcc ggggtagcga ttttatcgtg gacagtaaaa tccttaatgc tgccgatatt
     1981 ttgcttattg gtcaccagga tcaccggctg actgccggag gcggtacata ttttccattg
     2041 ggcccccttg tcccaaccga ctaaaaaagg tgatataccc ataaatccaa tatcaacttc
     2101 tccggccacc atggcatcac ggatgaccgg accggtcccc agctgtttcc agatgacttc
     2161 tgtgccgggc aggtattttt ccaccaattt ttgctgctga acgatctgca ggggagcgta
     2221 ggccagtccg tattggatgg caatggtgac cttttgttgg ggcgcatatg acccgtccgg
     2281 ctcattcttg ccgctgctgc agcccgcaat catgagcgac aggattaaaa caagtaccaa
     2341 atagtatttc ccccggtttc tcattctcat tcccctctcc tcttcaacga tagactcggt
     2401 agatgctgtg ggcgtcccag gtatccacca ccaggttttc cccctgcgga ttcaaaacct
     2461 gcagatgttg ataggcaaat ctcgttttgc gctgcaaatc catagggtcc ttgccttcca
     2521 tcagcaagta tccgattctc ttggtactgt ctaccaattc ttcgactctt tccatttcag
     2581 gatgataaaa tcctgcattg acgacgcccg gcagggtttt tattttttcc aagtcaccgc
     2641 agtgatcaac cattcccggg cgggcgacaa taaatttcac catggcattt ttacggatgg
     2701 cagatggatc gacaggatca aaattggtca ggctgatggc tttgcctgca gaacggtcca
     2761 gaaatagatc gactacgtca atcccagtca ccaatggaat cagctcttct tcatggcctc
     2821 ccccgatgcg gcaggctatt tccaccacct gaaattcacc gccgttgctc atcatctgta
     2881 tatataacgg accattttga atatcaaagc tgcgacatat tttatttacc agctcggtaa
     2941 tggctgtatg atgagaagtc caatagggtg acggatatac gtgcccatca ggcgtgccca
     3001 gatggggttc gatattaatc aatgggcgat cggttaccat aagtgttttt gcctgcccct
     3061 cgtccaccca ggcactgacc gtaatctcat ccccggcaaa gaattcttcc acaataattt
     3121 catcactgcg ggtataggca aaagtatgac gcatataatt cgccatcccg gcttgtaatg
     3181 ctggtaattt gaagatccct ctttgtccct gactgtcgag cggtttgacc accgccggga
     3241 accgtaatgg tgatagtttt tttcgcaaat gttcccaatc ctgaagctcg tctttatgaa
     3301 taaaacaatg gcggctgcag ggtatgtggt tggccgcaaa gcattccttc atgtattttt
     3361 tgttggtagc ccgcagggcc gtccagggcg tgatcatatg cgcaaggcca agggcttggg
     3421 cgacacaagc cgcagtatag accggctgat cggtgcctat ggcaaatact ccgtcaaccc
     3481 cgtattttct ggctacttca atgttagcct ccacatcaaa ggtactgacc agctctgcat
     3541 ggtcagccca gcgtttgccg ggcgcatccg gcaggtaatc agataccacc aatgtatagc
     3601 ccttgctttt ggccttgcga atcgcattca attgattgaa cccggccccc agacagagaa
     3661 gcttcacttc agttcccccc gtttacctcc aggcaggcaa tatcaaaatg aaatggccgg
     3721 ctggactgcg gcttggcaaa agcatagttg agatacaggt tcaaagccag acgcagggca
     3781 cttgccaagg tataattgct gctgccatat ttgcgtttct catgtttaac tgtgacatta
     3841 cctgaattgt tggtatattg aaagatcagg gctgcaaaat aaacaaatct tggttggttg
     3901 ttgagcattg attccgccag tggtctggtt aaaacccgat aactgctgct ttctatgtca
     3961 cgcggtttgg aaaacagcag cgttaaggaa gattgcatca aatggctgcc cagattgcga
     4021 taaaaagcct gtttttttct cgccgggacg gcaaagacca catcataacc gccgctttca
     4081 atcttggcca gcaaaagcgg gatttcttca ggaagctgct gcaaatcatc atccatggta
     4141 ataatcaggg cgccgcgact atattgcata ccacaaaggg tggccagatg ctgtccatga
     4201 ttgcaggcca gttgaatcat ctccacccgg ggatcttgct tgcgcaatga cctgataatc
     4261 tcaagactgt tatcctgact gcagtcgtcc accaagataa tttcaaaatc gcattgcaat
     4321 gattccagca ccctaaccag ccttgcatac agttcagcca ggctgtgttc actgttataa
     4381 accgggatga caacggatat ggatggcatc ctgatttgct cctttattac caaccggtca
     4441 gacattcctg caccttttca atcacatagg cttgttcatc ttcacttaaa tccggataaa
     4501 taggaagccg gattaaccgg cggctgatct cttccgtgac gggcaattca tccttgccat
     4561 aacctagtct tctgctcatc ggcgagctgt gcagagggat aaaatgaaaa ctggcttcca
     4621 ctccttgctc ccgcagactt tgcaataact catcccgctt tgcctgggtg ggacagagca
//
LOCUS       DAOTHZ010000121         4430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_23372_length_4430_cov_6.572571, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000121 DAOTHZ010000000
VERSION     DAOTHZ010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23372_length_4430_cov_6.572571"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="msrA"
                     /locus_tag="P5549_09965"
     CDS             complement(<1..866)
                     /gene="msrA"
                     /locus_tag="P5549_09965"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRW13437.1"
                     /translation="MDSTNTGMKLATLAGGCFWCLVPPYAELPGVIKVAAGYTGGHKE
                     KPTYEEVCYGNTGHIEAVQIRYDPQRISYDELLDVFWRQIDPTDAGGQFADRGTSYRT
                     AIFYHDEEQYQKAREAISRIDQSGRFTAPVATLLLPAQPFYPAEEYHQDYYKKNPDHY
                     NRYKAGSGREAFIKNFWTEAAKIPDPEHMADLAKKLTKLQFDVTQNNATEPPFRNAYW
                     DNHRAGIYVDIVSGEVLFSSLDKFDSGCGWPSFTKPLRKDSLQEKSDLSHGMRRKEVR
                     SQQADSHLGHVFP"
     assembly_gap    1140..1239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1335..2324)
                     /locus_tag="P5549_09970"
     CDS             complement(1335..2324)
                     /locus_tag="P5549_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRW13438.1"
                     /translation="MPIIDSDFKPHFRFRNAYIQCVLPTLLRKVESVHYTRQRVHTSD
                     GDFIDLDWSLATENKVPSESLAVICHGLEGSSDRAYMRGMAKAFNIRGIDTVAYNFRG
                     CSGETNWMKKSYSAGATDDIQDVLDAIKRAGNYQSIYLVGFSLGGNLVLKYGGEKGRD
                     IDPHIKGMAAISAPCDLQSSSVELLKSKNFIFSFRFLTMLRKKIREKAELYPEMKSID
                     IKAIKNLKQFDDQITAPLAGYKDAEDYWYKASCIRELDQIVIPAYIINAADDPILGSE
                     CYPYQIARSHPFIYLEVPARGGHVGFMHRPNQNEYWHETKTAEFLMRINNDDK"
     gene            complement(2548..>4430)
                     /locus_tag="P5549_09975"
     CDS             complement(2548..>4430)
                     /locus_tag="P5549_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW13439.1"
                     /translation="TVHMFYNTVKYCGHRPAQKYTNGSCYLTLTYQEFAERVENFGQG
                     FMALGLQKGDRISIMAPTNPIWDWADYGGRAAGCSVNTIYPDDSSETIIFTTNHSESN
                     CICVHDEVLLQKILNVWDKLPTVKHIVVMNSTYQSEIKNVMNMKELWDLGQAFRSGKP
                     NAYRERWQSLTQDDLCTILYTSGTTGKQKGCPLTHKNILSSTLGVSQVGYYGNTPFSV
                     DDTIFSILPLSHNWNRIDNHSACISYGGLIGYAQGPRTILQDFQEIKPTFVMLVARLW
                     DRIWNGVAGALCTTPEGKNKYEWAVNIGRKVLETRMDENGVIDLTQDPTLYLDSALKA
                     EFLQADREVFAFFRGVFGGRVNKAYSGGALLPADLQKNYWGMNFPLLDGWGLTETASG
                     INMVQARCVRIGWEAPQMAGSNMEVMLDPDDKEILVRGDSVIKEYYNNPEETALSFTP
                     DGWFRTGDIGEIDHGFLRIKDRKKAIIVLDTGKNVSPAYIEMKFTNNPYVEQILVVGD
                     NRKYISALVVPFYDNIINIFRQKDIAFDESKLAYATVNGMNTCVEVGDDLAGHPELYK
                     MIDEAINKVNDDLADFERIKRFKIIPRKFTEDRDELTPTLKIKNRIVFAHYPKEIEDL
                     YS"
BASE COUNT         1026 a         1109 c          984 g         1211 t
ORIGIN      
        1 ggaaaaacat gaccgagatg tgagtcagcc tgctgactgc gtacttcctt gcgacgcatg
       61 ccatgactca agtcgctttt ctcctgcagg gaatccttcc gcagcggttt ggtaaaactg
      121 ggccagccac atccggaatc aaatttgtcc agtgaactga acaaaacttc cccggagacg
      181 atgtccacgt aaatcccggc tcgatggttg tcccaatagg cattgcgaaa gggcggttcg
      241 gtcgcattgt tctgggtcac gtcaaactgc agcttggtca gctttttggc cagatccgcc
      301 atatgctccg ggtcaggaat tttggctgct tctgtccaga agttcttaat gaatgcttcc
      361 cttcccgatc cggccttgta gcggttgtaa tgatcaggat tctttttata ataatcttgg
      421 tgatactctt ccgccgggta aaagggttgg gcaggcaaca gcagggtggc cacaggagca
      481 gtaaaacgac cgctttgatc gattcggctg atagcctccc gggccttttg gtattgttcc
      541 tcgtcatggt aaaaaatcgc cgtgcgatag gatgtgcctc ggtcagcaaa ctgacctccg
      601 gcatcggtag gatcgatctg acgccagaaa acatccagca gttcatcata ggaaatgcgc
      661 tgcggatcat agcgaatctg caccgcttcg atgtgtccag tgttgccgta acaaacctct
      721 tcataggtgg gcttttcctt atggccgccg gtataaccgg ctgccacttt gatgaccccg
      781 ggcagttcgg catacggagg taccaaacac caaaaacagc ctccagccaa agtcgccagt
      841 ttcatgcctg tatttgtgct atccatgctt ttgtcccccc aaacttgttc tcttgcctga
      901 attgtaaatg cgcaacgcga ttcaggtttt cgcagcggtt ttcatttcat ccgggttgac
      961 tggcgagatc ccggccgcgg tttttattat atgtgggttg gccgttctca tcccggttac
     1021 gtttttcctg tgttgatgac cttgcccttt cggcttcccg tatgaaattg gttgagcggc
     1081 acgctttatt ttacatattc ccgatattgt tcttctttaa acccggtcag cagctttttn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tgcttcagga ggttacacag
     1261 taaaattcca atgtttttgc cattcataat catcctctct attatttttc gtattttctt
     1321 tgattagctc ttctctattt gtcatcgtta ttgatccgca ttaggaattc tgccgttttg
     1381 gtttcatgcc agtattcatt ctgattggga cgatgcataa accctacatg cccgccccgc
     1441 gctggaactt cgagatagat gaatggatgg ctgcgagcga tctggtaagg ataacattca
     1501 cttcccagga tcggatcgtc cgcagcgttt atgatgtaag caggaatgac aatttggtca
     1561 agttctctga tacaagaagc tttgtaccag taatcctcgg catccttata accggccaat
     1621 ggggccgtga tctggtcatc gaattgtttc aggttcttga tggctttgat gtcaatactc
     1681 ttcatttccg gatatagctc ggctttttct ctgatttttt tacgcagcat ggtaagaaat
     1741 ctgaaactaa aaatgaaatt ttttgatttg agcaattcga ccgaggaaga ttgcaaatca
     1801 cagggcgctg atatagctgc catgcctttg atatgcgggt cgatgtcccg gcctttttct
     1861 cctccgtatt taagtaccag attccccccc aggctaaagc ctaccaggta aatgctttga
     1921 taattgcctg cccttttaat tgcatccaac acgtcctgta tatcatccgt tgctcctgcc
     1981 gaatatgatt tcttcatcca attcgtttcc ccgctgcagc cgcggaaatt ataagcaacc
     2041 gtgtcaatgc cacggatatt gaaggccttt gccatccccc gcatataagc ccgatcagat
     2101 gatccttcca ggccatgaca aatgaccgcc agagattcag acggcacttt attttccgtc
     2161 gccagactcc aatccaaatc aataaaatca ccatcgctgg tatgaacccg ctggcgtgtg
     2221 taatgaacag actcaacttt tctgagcagc gtgggcagaa cgcattgtat ataagcattc
     2281 ctgaatctaa aatggggctt gaaatccgaa tctatgatcg gcattgatct gttccctttc
     2341 cctggtttca tcgctgggtt tttgataaac ctattatagc atttattcaa tggccaacat
     2401 ttaaccaaaa aagaatacca cccgcttaca caattaaaga tagcttgcgg gtctcgagaa
     2461 ccttgtgttc acaataaaaa ccggatagaa atatgaatca aactatgatt catacgctat
     2521 ccggcggtat gcggtatcct cttgtgttta ggaatataaa tcctcgattt ctttgggata
     2581 atgggcgaat acgatccgat tcttgatctt cagcgttggg gtcagttcat cgcggtcctc
     2641 ggtaaatttg cggggaatga tcttaaaacg cttgattctc tcgaaatccg ccaaatcatc
     2701 attgaccttg ttgatcgcct catcgatcat tttataaagc tcgggatgcc cggccagatc
     2761 atcgcctact tccacgcagg tattcatgcc gttgacggtg gcataagcca gtttggattc
     2821 atcaaaggcg atgtcctttt ggcggaatat attgatgatg ttatcataaa acggcacgac
     2881 caaagcgctg atatacttgc ggttgtcgcc caccaccagg atctgctcca cgtaggggtt
     2941 attggtaaac ttcatttcaa tataggcagg agatacattc tttccggtat ccaggacgat
     3001 gatggccttt ttgcggtcct tgatgcgcag gaagccatgg tcgatctccc caatatcgcc
     3061 ggttctgaac caaccatcgg gggtgaagct caacgctgtt tcttccggat tgttataata
     3121 ttctttgatg acgctgtcgc cgcgcaccag gatctccttg tcatcgggat caagcatcac
     3181 ttccatattg gagcccgcca tctgcggagc ctcccagcct atgcgcacac agcgcgcctg
     3241 caccatgttg atgcccgaag cggtttccgt cagtccccat ccatccagca gcgggaaatt
     3301 catcccccag taattctttt gcagatcagc cggcagcaag gctccgccgg aatatgcctt
     3361 gttgaccctc ccgccgaaaa cgccccggaa gaaagcaaaa acttcccgat ccgcctgcaa
     3421 aaactctgcc ttcagcgcac tgtccagata aagggtggga tcctgggtca gatcgatcac
     3481 tccgttttca tccattctgg tttccagtac cttgcggccg atattgaccg cccattcata
     3541 tttattcttg ccttccggag ttgtacacag tgccccggcc accccattcc agatgcgatc
     3601 ccagagacgc gccaccagca ttacaaaggt aggtttaatc tcctgaaaat cctgtaaaat
     3661 agtacgcggg ccctgagcat acccgatcaa accgccataa gaaatgcagg cactgtgatt
     3721 gtcaatgcga ttccagttgt gacttagcgg caggatggaa aagatcgtat cgtccacact
     3781 aaaaggagta ttaccgtaat agccaacctg gctaactccc agggtggagc ttaaaatgtt
     3841 cttgtgggtc aagggacaac ccttttgttt accggtagtc ccggaggtgt agaggatggt
     3901 gcaaaggtca tcctgagtca gggattgcca gcgttccctg taagcattgg gtttgccgct
     3961 ccgaaacgcc tgccccaaat cccagagttc tttcatgttc atgacgtttt taatctcgct
     4021 ttggtaggta gagttcatca ccacgatatg tttgaccgta ggcagtttgt cccagacatt
     4081 taaaattttc tgcagcagca cttcgtcatg aacacagatg cagttgctct cactgtgatt
     4141 ggtggtgaaa ataatggttt cactggaatc atccggataa atcgtgttaa cggaacaacc
     4201 agccgcccgt cccccataat cagcccagtc ccagatggga ttggtaggtg ccatgatcga
     4261 aatccgatcc cctttttgca accccagcgc cataaagccc tggccgaaat tctccacccg
     4321 ttcggcaaat tcttggtagg tcaacgtcag atagcagctg ccgttggtgt acttctgcgc
     4381 cgggcgatga ccgcaatatt tgacggtatt gtaaaacata tgaaccgtac
//
LOCUS       DAOTHZ010000122         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_23533_length_4404_cov_7.345367, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000122 DAOTHZ010000000
VERSION     DAOTHZ010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23533_length_4404_cov_7.345367"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="P5549_09980"
     CDS             complement(<1..262)
                     /locus_tag="P5549_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRW13440.1"
                     /translation="MTLPDLEFLKHRKQGLLGENKSFRSAVFLPLLELKGQPHVLFEK
                     RAADLKVQPGEICFPGGAIDKNDASPAQAAIRETSEELKIACD"
     gene            complement(316..834)
                     /gene="pssA"
                     /locus_tag="P5549_09985"
     CDS             complement(316..834)
                     /gene="pssA"
                     /locus_tag="P5549_09985"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRW13441.1"
                     /translation="MLLNLLNKSYANTITLLNIIFGSLSLIYTLHGDYTSAAVFILLA
                     VVMDGLDGKVARKLDIMSQLGKELDSLCDLVSFGVAPALLIYAQVLDPYIYSLGTFAA
                     VFFIVCGAFRLARFNVLNISDYFVGIPITLAGFLAAVLSLLAAWLPGNLILVLMLLLA
                     ILMVSNFKVRKF"
     gene            complement(815..1453)
                     /locus_tag="P5549_09990"
     CDS             complement(815..1453)
                     /locus_tag="P5549_09990"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRW13442.1"
                     /translation="MNHNEIIAREGWIFIAFFAGAAAALYCFAGWWGAVPAIILAVFC
                     LYFFRNPERFPSEGEKTVTSPADGKIMDIQEVQEDRFIHGKAVRVRIFLSLFNVHVNR
                     NPVDGKLVWLQKTGSKYLAAYKPEAADHNVRNYLGIEAFGKKILVVQITGLVARRLVC
                     WAAPGEYLQRGERFGLIRFGSCTELYLPTDALIKVKAGDKVKGGETDIAELA"
     gene            complement(1560..3290)
                     /locus_tag="P5549_09995"
     CDS             complement(1560..3290)
                     /locus_tag="P5549_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HRW13443.1"
                     /translation="MKSILLTTLNAKYIHSSLALRYLQAYCRDYGDLQLTIKEFTINE
                     AGNDVLAAIYQQQPQILCFSCYIWNIRLILELIEDYHKISPQTVIVLGGPEVSYDAEH
                     LLDSHPAIDYIVRGEGEAVLLELLLALTNQTEISPIKGVTYRQGDRSTSNPDQALIKE
                     LDTIPFPYPADMQTFRDKIVYYESSRGCPFHCSYCLSSTQHGVRFFSLPRVKQDLAFL
                     SRQGIREIKLVDRTFNCHEERAMEIMRWIISLETPTCFHFEIEASLLSECMLEFLATV
                     PAGKFNLEIGIQSTFQPALQAVNRRSAWSKVRDNIAQLRASRNFHIHLDLIAGLPLET
                     YHDFARSFNDVYQLQPDVLQLGFLKLLKGSAIRETADEHGYHFQTQPPYQVLNNRYLH
                     FAELIQLQQMEALLDKYHNTADMTKSLAYIVSKVYANDAFGFFQELTDYWQVRNLFAQ
                     GHKKDVYYQYLLEFMRDAHEAHLEIVHELLKYDYLMNNRHRLPACFSSCNSEDVNQAL
                     YDWIKDEQFRSLHLFDKQGQSHREIRKNLNLEYFSYDPETAEYQTDLLKIIFIYDPVK
                     KKAVRTVKVS"
     gene            complement(3315..4214)
                     /gene="murB"
                     /locus_tag="P5549_10000"
     CDS             complement(3315..4214)
                     /gene="murB"
                     /locus_tag="P5549_10000"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRW13444.1"
                     /translation="MFAELRRHLPVENVCENEPMRYHTSFKIGGPADLLVLPRNVEEV
                     KGVVTYCYQHSIPLLVIGRGSNLLVRDKGIRGVVMKLADNFQAFSLQAEQINAQAGIS
                     LYDLVRNAAAAALQGLEFAEGIPGSLGGAVVMNAGAYGGEMSDVVSEVQAIDEKGNIR
                     LFQKEELQYSYRRSVLQNSSYYVLGASLQLAPGNKAEIAARIQKYARQRADKQPLEMP
                     SAGSTFRRPDGYYVGPMIEELGLKGYPYGKAQISAKHAGFIVNRGGATADDVLTLIAI
                     VQKRVREKFGLDLQPEVRVVGEE"
BASE COUNT         1166 a         1125 c          962 g         1151 t
ORIGIN      
        1 catcacaggc gatctttaat tcttcactgg tttcgcgaat cgctgcctgg gccggactgg
       61 catcattttt atcgatggcg ccgccgggaa aacaaatctc gccaggctgc acctttaaat
      121 ctgccgctct tttttcaaac aatacatggg gttgaccctt cagttccagc agtggcaaga
      181 ataccgctga acgaaaagac ttgttctcac ctaatagccc ctgttttcga tgctttaaaa
      241 attccagatc aggtaatgtc ataaaacttc ttgcctccgt gtttatccat ctgatatcgg
      301 acgattctaa ttctcttaaa acttgcgtac tttaaaatta ctaaccatga gtatggccag
      361 caataacatt aagaccaaaa taagatttcc aggcagccag gccgccagca atgacaggac
      421 tgcagcaaga aaacccgcca gggtgatggg aattcccaca aagtaatcac tgatattcaa
      481 aacattaaac cgggccagcc gaaaggctcc gcaaacaatg aagaaaactg ccgcaaaagt
      541 ccccagcgaa taaatatagg gatccagaac ctgtgcataa atcagcagtg ctggagcaac
      601 gccgaaggat accaggtcac agagcgaatc caattctttt cccaactggg acatgatgtc
      661 cagctttctg gctactttgc cgtccaaccc gtccatgacg actgccagca ggataaaaac
      721 cgctgcgctg gtataatccc cgtgcaaggt atagatcagt gaaagcgaac cgaaaataat
      781 attcagcagc gtgattgtat tggcataact cttattaagc aagttcagca atatccgttt
      841 ctcctccctt caccttatca ccggctttga ccttgatcaa ggcatcggtc ggcaggtaca
      901 attctgtgca ggatccgaaa cgaatcaggc caaagcgctc tccccgctgc aagtattctc
      961 ccggggctgc ccagcatacc aatcggcggg cgaccagtcc ggtaatctgc accaccaata
     1021 ttttctttcc aaaagcctcg attcccaagt agtttcgcac attatgatcg gcggcttccg
     1081 gtttgtaggc agccaaatac ttactgccgg ttttctgcag ccaaaccagt ttgccatcaa
     1141 ccggattgcg gttgacatgg acattaaata aactcagaaa gatcctgacg cgaacagcct
     1201 tgccatggat aaaacggtct tcctgtactt cttgaatatc cattatcttg ccgtccgcgg
     1261 gggaggtaac cgttttttct ccttcacttg ggaagcgttc aggattgcga aaaaaataaa
     1321 gacagaatac agccagtatg attgccggta ccgctcccca ccatccggcg aaacaataca
     1381 gcgccgctgc tgcgccggcg aagaaggcaa tgaatatcca accctcgcgg gcaatgatct
     1441 cattatgatt cataaacaat cccatcccta tgtttcaaat aataattcat acaaactatc
     1501 tgcgattgta acattctctt atcaattcta ctgtaaaatc acaaatttgt cgcttatctt
     1561 tatgaaacct ttacggttcg aaccgctttt ttcttgaccg gatcgtaaat aaatataatc
     1621 ttcagcaaat cggtttgata ttctgccgtt tccgggtcat atgaaaaata ctccagattc
     1681 aaattcttcc tgatttcccg atggctttga ccctgtttgt caaacaggtg gaggctgcga
     1741 aattgttcgt ccttgatcca atcatacaac gcctggttca cgtcttccga attacaggaa
     1801 gaaaagcaag ccggcaagcg atggcggttg ttcatcagat agtcgtattt caacaattca
     1861 tggacgattt ccaagtgcgc ttcatgagca tcacgcataa attcaagcag gtattgataa
     1921 tatacatcct tcttgtgacc ctgtgcaaag agattccgga cctgccaata gtcagtcagt
     1981 tcttgaaaaa agccaaaggc atcattggca tataccttac tgacaatata tgcaagtgat
     2041 tttgtcatgt ccgcagtatt atggtatttg tccagcaaag cttccatctg ctgtaattgg
     2101 atcagctccg caaaatgcaa ataacggttg ttcaacacct ggtaaggcgg ttgggtttga
     2161 aaatgatagc catgttcatc ggctgtctcc cgtatcgccg atccttttaa caatttaaga
     2221 aatcccaact gcaatacatc cggttgcagt tggtaaacat cattaaagga acgggcaaaa
     2281 tcatggtacg tttccaaagg cagtccggcg ataagatcca aatgtatatg aaagttcctg
     2341 gaagctctga gttgggcaat gttgtcacgc accttgctcc atgccgacct gcgattgact
     2401 gcctgcaggg ctggttggaa agtggattgg atgccgattt ccagattgaa ctttcctgcc
     2461 ggtacggttg ccagaaattc cagcatacac tccgacaaaa gcgaggcttc gatttcaaaa
     2521 tgaaaacagg tgggggtctc cagactgata atccagcgca tgatttccat cgcccgttct
     2581 tcatgacaat taaaggtccg atcaaccagc ttgatttctc tgatcccctg tcgggacaga
     2641 aatgccagat cttgtttgac tctgggtaag gaaaaaaacc tcactccgtg ctgggtggat
     2701 gacagacaat aactgcaatg aaacggacag ccccgggagc tttcgtagta aacaattttg
     2761 tcccggaagg tctgcatatc ggcaggatat ggaaaaggta tggtgtcaag ctctttaatc
     2821 agcgcttggt cgggattgct ggtactccga tctccttgac gatatgtgac gcctttgatc
     2881 gggctaatct ctgtctggtt ggtcagcgcc agtaataact ccaatagcac agcctcccct
     2941 tctccgcgga cgatgtaatc aatcgctggg tggctgtcca gcagatgctc agcatcgtag
     3001 gacacttctg gaccgccgag gacaataacg gtttgcgggg agattttatg gtagtcttcg
     3061 atgagttcca atatgaggcg gatattccag atgtaacagg aaaagcagag tatctgcggt
     3121 tgttgctgat agatggccgc cagaacatca ttcccggctt cattaatggt aaactctttt
     3181 atagtcaatt gcagatcacc gtaatccctg caatatgcct gcaaataacg cagcgccagg
     3241 gaactgtgga tgtatttggc atttagtgtt gtcaacaaga tcgatttcac tggcttctcc
     3301 ttcttctagc ttgcttactc ctcacctacc acccggactt ccggctgcag gtcaaggcca
     3361 aatttttccc tgacccgctt ttgcacaatg gcaatcagcg tgagaacatc gtcggctgta
     3421 gcaccgcccc ggttgacaat aaaaccggca tgtttggcag agatctgggc tttgccgtac
     3481 ggatagcctt tcaagccaag ttcttcgatc atcggaccca catagtaacc gtccggtctt
     3541 ctgaaagtac tgccggcact gggcatctcc aggggttgtt tgtcagcccg ctggcgggca
     3601 tatttctgta tgcgggcagc aatttccgct ttgttgcccg gggcaagctg caaacttgca
     3661 cctaatacat aatagctgct gttttgcaaa acactgcggc gataggaata ttgcagttct
     3721 tctttttgaa acagccttat atttcccttt tcatcaatcg cctggacttc actgacaaca
     3781 tcgctcattt caccgccata ggcacctgca ttcatgacca ctgctccgcc caggctgccg
     3841 gggatgcctt cagcaaactc caaaccttgc agcgcagcag cggcagcatt gcggaccaga
     3901 tcatacagac tgatccccgc ctgggcatta atctgctctg cctgcaggga aaacgcctga
     3961 aaattatcgg ccagtttcat gaccaccccg cgtatcccct tatcccgaac cagcaggttg
     4021 ctgccccgcc cgattaccag cagcggaatg gaatgctgat agcaataggt taccacgcct
     4081 ttaacttcct ccacattgcg gggcaacacc agcagatcag ccggcccgcc gattttaaaa
     4141 ctggtgtgat atcgcatcgg ttcattttcg caaacatttt caaccggcaa atgacgccgc
     4201 aattctgcaa acatgtcaat actccttaca tgaaaatagg cgggaacttt ggtgtgcaca
     4261 atgcaaagaa aaggtcccta atcaattttt atggaaatct ctctgattta ttcacttctg
     4321 ttcaatttga ctgcgatttt gggagcttcg ggcatgacaa tgacccgggc ttcttctccg
     4381 tgtcgtttga atgcataacg caac
//
LOCUS       DAOTHZ010000123         4313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_24220_length_4313_cov_6.085251, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000123 DAOTHZ010000000
VERSION     DAOTHZ010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24220_length_4313_cov_6.085251"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(559..1188)
                     /locus_tag="P5549_10005"
     CDS             complement(559..1188)
                     /locus_tag="P5549_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRW13445.1"
                     /translation="MALFGRKDENLFFLFEESSRVVVRGGDILNDIIADYRDLDVKMS
                     KLLDLENEGDRIIEQLVGRLNASFIMPFDREDAFQLVQKLSVTLDHITGIIDVIILYK
                     TGQPNQRVREMVEVLYDALLLQQKAFKLIDRMESNKKTIFECCEEIRSLERKQDNLYR
                     NGLADLFEKEKDKPLNIIKWREVYEHIESATDHVEDVGNLIRNICVKYS"
     gene            complement(1296..2174)
                     /locus_tag="P5549_10010"
     CDS             complement(1296..2174)
                     /locus_tag="P5549_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW13446.1"
                     /translation="MRYEGNVFRPPSEAYSYILQCTVGCSHNRCTFCAMYIDKKYHVR
                     KMEDIKEDIRMAKQYYGDLEKVFLADGDALAMPTDDLLEILDTIYKTFPSLKHVGIYA
                     SPESILEKEMSELTALKAAGLTIAYLGVETGDPELLEAIRKGANYDEMLEAGRKIRRA
                     GILLSVTILLGLAGRTPKAVEHEKNTAKMMNEMNPDYIGALTLMLVPGTQLYRKQQKG
                     EFELPGPFEILDEMRILIENLDVQGTEFRSNHASNYLPIKGRLPEDKQKMLDLINDMI
                     NKNDRKYLRPDYLRAL"
     assembly_gap    2235..2334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2487..2678
                     /locus_tag="P5549_10015"
     CDS             2487..2678
                     /locus_tag="P5549_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HRW13447.1"
                     /translation="MARNRSIMSEQLKYEIARELGVDSIVATQGWGAVSSRDCGNMVK
                     SAIQIAERSIAGQNNQNLL"
     gene            complement(2831..4285)
                     /gene="guaB"
                     /locus_tag="P5549_10020"
     CDS             complement(2831..4285)
                     /gene="guaB"
                     /locus_tag="P5549_10020"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRW13448.1"
                     /translation="MNDKFSKEGLTFDDVLLIPGHSTVLPNEVDVSTRLTRNIRLETP
                     IMSAGMDTVTESRMAIAMAREGGIGILHKNMSIEEQAKMVDRVKRSEHGVITDPFFLS
                     PEHKVSDALQIMEHYRISGVPITEGPKLVGILTNRDLRFETDFTQPIKKIMTSTNLVT
                     APVNTTMEEAMQLLRKYKIEKLPLVDENYHLKGLITIKDIEKTMKYPNSAKDNRGRLL
                     VGAAVGIAKNTMERTQALINAGVDVIVVDTAHGHSQGVIEMVTAIKKEFPDLELIAGN
                     VATGEATEALIKAGADCIKIGIGPGSICTTRVVAGIGVPQITAVVDCSQVARKYDIPV
                     IADGGIKYSGDIAKAIAAGADVVMLGNLLAGTDESPGEEIMLQGRRFKVYRGMGSMGA
                     MVEGSSDRYFQEDAQKLVPEGIEGRTPYKGAVSETIFQLVGGLRAGMGYCGVANIKEM
                     QEKTRFVKISNAGLVESHPHDVVVTKEAPNYQIM"
BASE COUNT          895 a          990 c         1138 g         1190 t
ORIGIN      
        1 tgaatctgta cctcagccca gtgaccactg acatcactca attggctggc ggctgttgcc
       61 gacggtactg cgcaaaatat ggcaaatacc atcatgacag cgatgataaa acttaaacgt
      121 ttcttctttt gcaaacccta ataccccctt aaaatatttt tcatatatta tgactgttaa
      181 aaagtttcat gtagtttacg atcaccccca aagtgtaatg caaccaggca tcgcctaatg
      241 tttcattaaa ttaatttaca catggcccat gattggagta ttgtttttgt atattttcct
      301 atatattacc atgggatttg acaaagtgta aatttttgtc atttattgcg ttgatgagga
      361 aaaagaagcg attgaattat ggtatttctc gatattatgg gcctttttta attataagcc
      421 cgcagctttt ttctagcaag cattatttgt gctttctggg aatggatgcc tataaatccg
      481 tccccagcct atttgccgac gacatctgtt gatgaaaagc aattgggaat ttcgaattgc
      541 taacttgtac gtaaatgatc aagaatactt gacgcagatg ttgcggatga ggttgccgac
      601 atcttcaaca tggtcagtgg cactctctat atgttcgtaa acttcgcgcc atttaatgat
      661 gttgaggggc ttgtcttttt ctttttcgaa caaatccgcc aggccattgc ggtaaaggtt
      721 gtcttgtttg cgttcaagac ttctgatttc ttcgcagcac tcgaagatgg tttttttgtt
      781 gctttccatg cggtcgatga gtttaaaggc tttttgctgc aggagcaggg catcgtagag
      841 gacttcgacc atttcccgga ctctttggtt gggctggccg gttttgtaca gaatgatgac
      901 gtcgatgatc ccggtaatgt ggtccagggt cacagacaat ttctgtacca gctgaaacgc
      961 gtcttcgcgg tcaaagggca tgatgaaact ggcgttcaag cgtcccacca actgctcgat
     1021 gatgcggtcg ccttcgtttt caaggtcaag cagtttggac atttttacat ccaaatcgcg
     1081 gtaatcggcg atgatgtcgt tgaggatgtc ccctccgcgg acgacgactc ttgagctctc
     1141 ttcaaaaagg aaaaacaggt tttcatcctt tctgccaaac agtgccataa tttacccccc
     1201 aatctctatg cgataagcgg gattgctccc gttaccagcc gatgcatcac agcgcgcgca
     1261 agtcaagaac cgtccccgac ttgcgctgat ttgcgttata gggcgcgcaa gtaatccggc
     1321 cggaggtatt tgcggtcgtt tttgttgatc atgtcattga tcagatcgag cattttttgt
     1381 ttgtcttcgg gcagccggcc tttgatgggc agatagttgg aagcatggtt gcttctgaac
     1441 tcggtgcctt gtacgtccag gttttcgatg aggatgcgca tttcatccag gatttcaaag
     1501 ggaccgggca gctcgaactc gcctttttgc tgtttgcggt agagttgggt gccgggcacc
     1561 agcatgagag tcaatgcgcc gatataatcg ggattcattt cgttcatcat tttggcggta
     1621 tttttttcgt gttcgacggc tttgggggtt cgtccggcca ggcccagcag gatggtcacg
     1681 gacagcagga tgccggcccg ccggattttg cggcctgctt ccagcatttc gtcataattg
     1741 gcgcctttgc gaatggcctc cagcagttcg ggatcaccgg tctcgactcc caaataggcg
     1801 atggtcaagc cggctgcctt gagcgcggtc agctcggaca tttctttttc caggatgctt
     1861 tccgggctgg cgtaaatgcc gacgtgtttt aagctgggaa aggttttata aatggtgtcc
     1921 aaaatctcca gcaaatcgtc ggtgggcatg gccagagcgt cgccgtcagc caaaaacact
     1981 ttttccagat cgccatagta ttgtttggcc atgcggatgt cttctttgat gtcctccatc
     2041 ttgcgaacgt gatatttttt atcgatgtac atggcgcaga aggtgcagcg gttgtgggaa
     2101 caaccgacgg tgcactgcag aatataactg taagcctcac tgggaggccg gaatacgttg
     2161 ccttcatatc tcataattcg attccctcct actttaatga ttataacagg aaaagctgtg
     2221 aaggtgacag atttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgtcat
     2341 cctgctcgtc ggtggcgcta cagtgctgaa ttttttttga aatatgcaag aacatcactt
     2401 catctattca tagaaattag ctgtatgatt tgggcgtttt caacacaaac tatgggtgtg
     2461 cgaggcatga tgaggaggtt ccgcgaatgg cccgtaatcg aagtatcatg tccgaacagc
     2521 tcaagtatga gattgcccgt gaacttggcg tcgacagtat tgtcgcgacg cagggctggg
     2581 gcgccgtaag ctccagggat tgcggcaaca tggtaaaaag tgccatccaa attgctgagc
     2641 gcagtattgc tgggcagaac aaccagaact tgctgtagtg tagctcgcac acaggagccg
     2701 gaggaacgta ctccggctcc ttctttttgt tgtaagcgag gaacgattct cgcgcaagtt
     2761 ggggacggtt cttgccttgc gcgttttggg ttcctgaccg cgcaaggcaa gaaccgtccc
     2821 cgacttgcgt ctacatgatc tggtagttgg gggcttcctt ggtaacgacc acgtcgtggg
     2881 ggtggctctc gaccaggccg gcgttgctga tttttacgaa acgggttttt tcctgcattt
     2941 ccttgatgtt ggctacgccg caatagccca tgccggcgcg cagtccgccg accagctgga
     3001 agatggtttc ggagacggca cctttatacg gggtgcggcc ttcaatgcct tcgggcacca
     3061 gtttctgggc atcttcctgg aagtaacggt cactgctgcc ttccaccatg gcgcccatgg
     3121 agcccatgcc gcgataaact ttgaaacggc gtccctgcag catgatttct tcgccggggc
     3181 tttcgtcggt gccggccagc aggttgccca gcatgacaac gtcagcgcct gcggcgatgg
     3241 ctttggcgat gtcgccggag tatttgatgc cgccgtcggc gatgacgggg atgtcatatt
     3301 ttctggccac ctgggagcag tcgaccacgg cggtgatctg gggtacgccg atgccggcga
     3361 ctacgcgggt ggtgcagatg gaaccggggc cgatgccgat tttgatacag tcggcgccgg
     3421 ctttgattaa ggcttcggtg gcttcaccgg tagctacgtt gccggcaatc aattcaagat
     3481 ccgggaattc tttcttgatg gcggtcacca tttcgatgac cccctgggaa tggccgtggg
     3541 cggtatcgac cacgatgacg tcgacgccgg cattgatcag cgcctgggtt ctctccatgg
     3601 tatttttggc gatgcccacc gcggctccga ccagcagacg accgcgatta tctttggcgg
     3661 agttgggata tttcatggtt ttttcaatgt ctttaatggt gatgaggcct ttcaggtgat
     3721 aattttcatc caccaggggc agtttttcga ttttgtattt gcgcagcagc tgcatggctt
     3781 cttccatggt ggtattgacc ggcgcggtga ccagattggt gctggtcatg atttttttaa
     3841 tgggctgggt gaaatcggtc tcgaaacgca gatcacggtt ggttagaatg ccgaccagtt
     3901 tggggccttc ggtgatgggt acaccggaaa tgcgataatg ttccatgatt tgcagggcgt
     3961 cgctgacttt gtgttcgggg gagagaaaga agggatcggt gatgacgccg tgctcggagc
     4021 gtttgacgcg gtccaccatt ttggcctgtt cttcgatgct catgttcttg tggagaatgc
     4081 cgatgccgcc ctcgcgggcc atggcaattg ccatgcggga ctccgtaacc gtatccatgc
     4141 cggcgctcat gatgggagtt tccagtctta tattacgggt caaacgggtg gatacatcca
     4201 cttcgtttgg caacacggtt gaatgtccgg gaatcaacag gacgtcatca aaggtgagac
     4261 cctctttgct aaatttgtcg ttcattgtct ggcctccttc tataaaagtt aaa
//
LOCUS       DAOTHZ010000124         4239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_24747_length_4239_cov_6.856358, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000124 DAOTHZ010000000
VERSION     DAOTHZ010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24747_length_4239_cov_6.856358"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1073)
                     /locus_tag="P5549_10025"
     CDS             complement(15..1073)
                     /locus_tag="P5549_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRW13449.1"
                     /translation="MIRQEIEQRETVMLHPLATPAVLSRGRSMAEEPDPIRTCFMVDR
                     DRIIHSKSFRRLKHKTQVFIAPPGDHYRTRLTHTLEVNQIAKTIGAGLNLNLDLIEAI
                     ALAHDVGHTPFAHAGEQILDELVSGRFRHNENSIRVLTRVEQHKASSGLNLCEEVLDG
                     VLHHSGYGENVPAAATLEGQVIRLSDKIAYVQHDIDDSIRAGLLVLEQIPREYREVLG
                     ETHSKRIATLVTDTIHHSRSLMEAGIHPRVEPGPEKQKALLGLREFMFENVYRGPVCL
                     AERRRAMFIIEQLFHYYKQHPGRMSCLYQQIADEEGIEAAVVDYISGMSDDYCIAMFE
                     DIYIPHSHVLYPETIREE"
     gene            complement(1257..1577)
                     /gene="sugE"
                     /locus_tag="P5549_10030"
     CDS             complement(1257..1577)
                     /gene="sugE"
                     /locus_tag="P5549_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HRW13450.1"
                     /translation="MQWVLLVIAGLLEMGWAVGLKYTQGFTKLIPSVFTVMGMMASFC
                     FLSLALRSLPLGTAYAVWTGIGTVGIVILGIILFKEPVDLLRLLFIGLIVIGIIGLKM
                     VFPR"
     regulatory      complement(1632..1733)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            complement(1871..>4239)
                     /gene="ppdK"
                     /locus_tag="P5549_10035"
     CDS             complement(1871..>4239)
                     /gene="ppdK"
                     /locus_tag="P5549_10035"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRW13451.1"
                     /translation="RSGAAVSMPGMMDTILNLGLNDESVLGLAELTGDDRFAYDCYRR
                     FIQMFSDVVLEISHSRFGEIVEKYKRKLGVIFDYEIPAAEMKNMIQEFKDLVLKEKGF
                     PFPQEVNEQLERAIRAVFDSWNNQRAIVYRRINKIPDDLGTAVNIQCMAFGNMGQDCG
                     TGVAFTRNPSTGEKLLYGEFLVNAQGEDVVAGIRTPMPISKLQEELPGVYQQFVDTCN
                     KLEQHYRDLQDIEFTVEKGKLYMLQTRNGKRTTRAAVKVAVDMVKEGLITQEEALLRV
                     DPEQINQLLHRQIDTSVELEPIAQGLPASPGAACGKVVFDADIAEKLGEAGEKVLLVR
                     TETTPDDIHGIVYAQGVLTSRGGMTSHAAVVARGMGKPCVCGCESIKIDLAADQFVVD
                     DIVVKAGEIITMDGSTGKVMRGEVPMIEPTLSDEFEVLLNWANQTKKLSVRANADTPE
                     DAAKAREFGAEGIGLCRTEHMFMAQERLPIVQEMILSENLADREAALDKLLPIQRQDF
                     YGIIKAMSPLPVCIRLLDPPLHEFLPSREELMVEIVEMKHKQASLREIAEKEELLKKV
                     NKLHEANPMLGHRGCRLGITYPEIYIMQVRAIFQAMVQLHQEGCKNYVEIEIPLVMDQ
                     AEFLLLKDHILEVYASIKQENNVELDFALGTMLELPRACVMADEIAQQAEFFSFGTND
                     LTQTTLGFSRDDAEGKFIPVYLEKKIIKDNPFAVLDRKGVGALMRLAAEKARNTRPEI
                     ILGICGEHGGEPSSVEFCHLLGLDYVSCSPYRIPIARLAAAQAAINNK"
BASE COUNT         1022 a         1120 c          975 g         1122 t
ORIGIN      
        1 tttataagtt ttgtttattc ttcgcgtatc gtttccgggt acaatacatg ggaatgaggg
       61 atataaatat cttcaaacat ggcaatacaa taatcatcac tcatacctga gatataatcc
      121 accaccgccg cctcaatgcc ctcttcgtca gcgatctgct gatacagaca gctcatgcgg
      181 ccgggatgct gtttatagta gtgaaataac tgttcaataa taaacatggc acgacgtctt
      241 tcagccaggc aaaccggacc ccggtagacg ttttcaaaca taaattccct aagccccagc
      301 aaggcttttt gtttttccgg tccgggctcc acccggggat gtatccctgc ttccatcaaa
      361 ctgcggctgt gatgaatcgt atcggtgacc aaagtggcga tgcgtttgct gtgggtttct
      421 cccaaaactt cccgatactc cctgggaatt tgctccagca ccagcaggcc ggcacggatg
      481 gaatcatcaa tgtcatgctg tacataggca attttgtcac tcagtctgat tacctgaccc
      541 tccagggtag cggctgcggg tacattttct ccatacccgc tgtggtgcag cacaccgtcc
      601 agcacttcct cgcaaaggtt aagtccgctg ctggctttgt gctgttcgac ccgggtgagc
      661 acccggatgc tgttttcatt gtgacgaaaa cggccgctga ccaattcatc caggatctgc
      721 tcgccggcat gggcaaaggg agtgtgtcct acatcatggg ccagcgcgat ggcctcgatc
      781 agatccagat taaggttcaa gccggcaccg atggttttgg cgatctgatt gacttccagg
      841 gtatgggtca gtcgggtccg gtaatgatcg ccgggcggcg cgatgaacac ctgagtcttg
      901 tgtttcagcc ggcgaaacga tttggaatgt atgatccgat cccggtcaac catgaaacag
      961 gtacggatgg ggtcaggctc ctccgccatg cttctgcccc gggaaagaac cgccggggtc
     1021 gccagcggat gcagcatcac tgtttcccgt tgttcgattt cctgcctgat cattccgtct
     1081 cacctcgtta aattgtggta tcacaccatt atttacctgt cttatacaag atcttgtaca
     1141 caaacatcag ttcactgttt ttcaattaat actacaatcg tcaattgaaa tcctttaatt
     1201 catcaaattt ttatctttcg ttaacaaaag tttaacaaaa agttttttcc ctatcactaa
     1261 cgaggaaata ccattttcaa gccaataatt cctataacaa tcaatccaat aaagagtagc
     1321 cttaacaggt ccacgggctc tttaaacaaa atgattccca gaattacgat acctactgta
     1381 ccgattccgg tccaaaccgc ataagctgtt cccaagggaa gactcctcaa cgccaacgat
     1441 aaaaaacaaa agctggccat cattcccata accgtaaaaa cactcggtat cagctttgta
     1501 aaaccttgcg tatattttaa acctactgcc caacccattt ccaaaagccc tgcgattacc
     1561 aataacaccc attgcatgtt cttccatcct ttcagtaaaa aaatataaaa aacccggaag
     1621 atatgcatga tatctcccag gcttttatcc ttccgtgaac acagctgact gtgcgtttta
     1681 tctctgtcca gaccagtatt ttgcaaaact gcggaaccct ataaagcttt agaatatgaa
     1741 attttctcag acaatcgtat caaaaagaat atataagttc atgcttgttc actcacgagc
     1801 cggtgttact gaccggaact tcccaagctt ttttaatgaa agcctgacaa cttttctccc
     1861 gctggcagcc ttacttgttg ttaatggctg cttgggcggc tgcgagacgg gcaatgggaa
     1921 tgcgataggg ggaacagctg acataatcaa gtcccagcag atgacagaat tccaccgaac
     1981 tcggttctcc cccatgttct ccgcagatcc ccaggatgat ttccgggcgc gtgttgcgtg
     2041 ccttctcggc ggccaacctc atcagcgcac ccaccccttt gcgatccagt acggcaaaag
     2101 gattgtcctt gataatcttc ttttccagat agaccggaat gaacttgcct tctgcatcgt
     2161 cgcggctgaa gcccaaagtc gtctgggtca gatcgttggt gccgaaagag aagaattccg
     2221 cctgttgcgc aatctcgtct gccatgacac aggctctggg cagttccagc atcgttccca
     2281 gggcaaagtc aagctcgaca ttgttttcct gtttgatgga ggcatagact tccaggatat
     2341 gatcctttag caggaggaac tcagcctgat ccatgaccag cggtatttct atttccacat
     2401 agtttttgca gccttcctgg tgtaattgca ccatagcctg gaaaatagcc cggacctgca
     2461 tgatgtagat ttccggatag gtgatgccca ggcggcagcc gcggtgtcca agcatgggat
     2521 tggcttcatg caatttgttg acttttttga gcagttcttc tttttccgcg atttcccgca
     2581 gggaagcctg tttatgcttc atctccacaa tttctaccat taactcttca cgagagggca
     2641 ggaattcatg caggggcgga tccagcaggc gaatgcacac cggcagcggc gacatggctt
     2701 tgatgatacc gtaaaaatcc tgtctctgga tgggtaatag tttgtccagc gcagcttccc
     2761 gatcagccag gttttcggag agaatcatct cctgcacaat gggcagacgc tcctgggcca
     2821 taaacatatg ctcggtgcgg cacaatccga tgccttctgc accgaattcc cttgctttgg
     2881 cagcatcctc aggggtatca gcattggctc tgaccgacag tttcttggtt tggttggccc
     2941 aattgagcag cacttcaaat tcatcggaaa gggtaggctc aatcatggga acttcacccc
     3001 gcattacttt gccggtggaa ccgtccatgg tgataatttc gccggctttg accacgatgt
     3061 cgtccacaac gaactggtct gccgccaggt cgattttgat actctcgcag ccgcagacac
     3121 aaggtttgcc catgccgcgg gctaccaccg ccgcatggga ggtcatgccg ccacgggatg
     3181 tcaaaacccc ctgggcgtag acgattccgt gaatatcatc gggggtggtt tcagtgcgga
     3241 ccagcaacac tttttcgcct gcctcaccta atttttcggc gatatcagca tcaaacacca
     3301 ccttgccaca ggctgcccct ggcgaagcag ggagtccttg ggcaatcggt tcaagttcaa
     3361 cggaagtatc gatttgtcta tgtaacaatt ggttgatctg ttcagggtca acccgcagca
     3421 acgcttcctc ctgggtaatc aagccttcct tgaccatatc tacggcgact tttacggctg
     3481 cccgggtggt tcttttgccg ttgcgggtct gcagcatata caatttgcct ttttccactg
     3541 taaattcaat atcctgcaga tcgcggtaat gctgttccag tttattgcag gtgtctacaa
     3601 attgctgata gacaccgggc agttcttcct ggagtttgct aatcggcatg ggggtgcgaa
     3661 ttcctgccac cacatcttcg ccctgggcgt tgaccaggaa ttctccatag aggagtttct
     3721 ctccggtgga agggttgcgg gtaaaagcaa ctcccgttcc gcagtcctgt cccatgttac
     3781 cgaaggccat gcattgaata ttgacggccg tgcctaaatc atcaggaatt ttgttaatcc
     3841 tgcggtagac gatggcgcgc tggttgttcc aggaatcaaa aaccgcccgg atggctctct
     3901 ccagctgttc atttacctcc tgggggaagg ggaacccttt ttctttaagg accaggtctt
     3961 tgaattcctg gatcatattc ttcatttccg cagccggaat ctcataatca aaaataaccc
     4021 ctagtttgcg cttgtatttc tccacaattt ctccaaaccg ggagtgactg atttccagaa
     4081 cgacatcgga gaacatctga ataaaacgcc ggtaacagtc gtaggcaaat ctgtcgtcgc
     4141 cggtcaattc agccagaccg aggacggatt catcattgag accaagattg aggatggtat
     4201 ccatcatgcc cggcatggaa actgccgctc cggaacgaa
//
LOCUS       DAOTHZ010000125         4167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_25322_length_4167_cov_7.673881, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000125 DAOTHZ010000000
VERSION     DAOTHZ010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25322_length_4167_cov_7.673881"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..926
                     /locus_tag="P5549_10040"
     CDS             387..926
                     /locus_tag="P5549_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13452.1"
                     /translation="MSEHPHIPTKILFYIGIAVGLIICILMVLATTFDIHTTWPAYLT
                     LPIFFLAGNDTKQLPHLFIGACSGLIFTALFFIAYAALVPVLGAHLAPLLLVFVILVL
                     IIGLGNVVPMVFNNYNLVYFTVAIAFYTVMFAINPAYPLIPETLKMIGITLVGGGAFV
                     GGTLLFIGKILPKILAPKA"
     gene            1207..2637
                     /locus_tag="P5549_10045"
     CDS             1207..2637
                     /locus_tag="P5549_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRW13453.1"
                     /translation="MITSQEYLVRIAKMRPNIYVDGEVIGRDHPLVLHASKIIQSTFD
                     LAQDPEYSKWLKTTSNISGKEINRFTHIHQSVEDLLLKQEMTRKLCNRSGGCIYRCMG
                     TDSMNALSVTTKNTDIKYGTNYHERFLNFLAYFQENDLVGAAAQTDAKGNRSLRPSEQ
                     PDPDAYLRVVERRADGVVVRGAKLHNTMAPYADEILAFPTRALKKNEQDYAIAFAIPA
                     DAEGIYLIGRKEYSHPREEGMEAPYGDLACIESFTVFDNVFIPNERIFLNGETEFGSE
                     LAMMFALYHRHSYCGCKPGVGDVMYGLTALLADYNGVEKTGHTREKLADVASVVELVY
                     AAGIAASVKSRKAPSGTQIPNIIYCNVGRRHAGHNVYHEYDILCDIAGGMPATLPLSK
                     EYNSPIVGDFVRKYTRRREGVSDENHYRAYHLASDILNGEMGAALLVAGVHGGGSPIM
                     EDIAILGSYDTEGKKNLARHLAGIKE"
     gene            2765..>4167
                     /locus_tag="P5549_10050"
     CDS             2765..>4167
                     /locus_tag="P5549_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRW13454.1"
                     /translation="MITSQQYLDRIAKMRPNIYVDGEVIGRDHPLVLKASQIIQSTFD
                     LAEDPEYSKWLKTTSHISGQEISRFTHIHQSVEDLLLKQEMTRKLCNRSGGCVYRCMG
                     CDAMNALSVTTKNIDLKYGTDYHDRFLKYLAYFQENDLVGCAAQTDAKGNRSLRPAEQ
                     PDPDAYLHVVERRADGVVVRGAKLHNTMAPYADEIIALPTRALKKNEGDYAIAFAIPA
                     DTEGIYLIGRKAFSQSREEGLEAPYTKLGSIESFTVFDNVFIPNERIFLDGQTEFGSE
                     LAMLFALYHRHSYCGCKPGVGDVMYGLTALLADYNGVEKTSHTREKLAEIASVVELVF
                     AAGVAASVKSRKAPSGTQIPNSIYCNVGRRHAGHNVYHEYDILCDIAGGLPATLPLSK
                     EYNSPVVGDFVRKYTKRREGVSAENQYRAYNMASDILNGDVGAALLVAGVHGGGSPIM
                     EDIAILGTYDTEGKKKLA"
BASE COUNT         1118 a          912 c         1086 g         1051 t
ORIGIN      
        1 tagataccaa aaagaatcaa aaccaggtaa atctatcatg tgaaatcggc aacaacttac
       61 taaccccaat ttatcgagtc ttggaacaat tttttcgaat tctgtacacc ccgatgcaga
      121 agtgtatcaa ttaataattg aattgccggt aaaagaatat ttgaatggtt gggaatgctg
      181 ataagttggc attagtaagg catttaagag acatcgcaga aattatttca tactgtcata
      241 ctggcataat atttgcaatg tttagattgt aatgaaatct taatgcttat tctttggaaa
      301 gtctgacagg aaagggaggg gagtattaaa acaaggttag tcctggttgt ttaagcgaag
      361 caaattatga aagggttggt gaaagcatgt ctgaacatcc acatattccg acaaagattc
      421 tcttttatat tggaattgca gtaggcttga ttatttgtat tttgatggtg ttggcaacca
      481 cattcgatat tcacacgact tggccagcat atttgacatt gccgattttc ttcctggcag
      541 gaaatgacac caagcagtta ccgcacttgt ttatcggtgc ttgttccgga ttgattttta
      601 ccgcactgtt ctttatcgcc tatgctgcct tggtaccagt tctgggtgca catcttgctc
      661 ccctgctgct ggtatttgtg attctggttc tcatcattgg actgggcaat gtggtgccga
      721 tggtattcaa caactacaat ctagtctatt tcaccgtggc gattgctttt tacaccgtta
      781 tgttcgcgat caatccagcc tatccgttaa tccctgaaac attgaaaatg attggcatta
      841 ccttggtggg cggcggcgca tttgtgggcg gcaccttgct ctttatcggc aagattctac
      901 ccaaaatttt ggcacccaaa gcgtaaggtg tgtcttttgt tttaaaaatt aaacgcagta
      961 aggttcatga agtccttggt tggcttattt tacagaagcc tccaagggct tcattgtttt
     1021 aggataatcg ggtttgatta accaacagca ggacaatgtt ggtacaaaaa ttgcataata
     1081 tttgggtgcg gatgtacgca tagggcgatg gcttaaaaca cagtgtcagc gcaggtgtgt
     1141 taaagaatag tgccatgtta tattgaagtt gttgatcaaa atccagcaaa aaggagggct
     1201 tatataatga taacatccca agaatattta gtgagaattg ccaaaatgag acctaatatt
     1261 tatgttgacg gggaggttat cggcagggat catccgcttg tcctgcatgc cagtaaaatc
     1321 atccagtcaa catttgatct ggcccaggat cctgagtaca gcaaatggct gaaaaccacc
     1381 tcaaatatca gcggcaagga aatcaaccgg tttacccata ttcatcagtc ggttgaagat
     1441 ttgttgctga agcaggaaat gacccgcaaa ctatgcaatc gcagcggcgg gtgcatttat
     1501 cgctgcatgg ggactgactc gatgaatgcg ttgtcggtga cgaccaaaaa caccgatatc
     1561 aaatacggaa ccaattatca tgaacgtttt ctgaatttcc tggcatattt ccaggaaaat
     1621 gatctggtgg gggcagcggc ccagaccgac gccaaaggca atcgcagtct ccggccttcg
     1681 gaacagccgg acccggatgc ttacctgcgg gtggtggaaa gaagagcgga cggggtcgtt
     1741 gttcgaggag ccaagctgca caataccatg gcaccctatg ccgatgaaat tttggctttc
     1801 ccgacacgcg ccttgaagaa aaacgagcaa gactatgcga ttgcttttgc cattcctgct
     1861 gatgccgaag gaatctatct gattggtcgc aaggaatatt ctcatcctcg tgaagaaggc
     1921 atggaggcac cttatggcga tttggcctgt atcgagagtt ttaccgtttt tgacaatgtt
     1981 tttattccca atgagcgaat tttcctaaac ggcgaaaccg agttcggcag tgaactggcg
     2041 atgatgtttg cgctttatca ccggcacagt tactgcggct gcaaacctgg cgtaggagat
     2101 gtcatgtatg ggctgaccgc attgctggct gactacaacg gggtggaaaa aaccggccat
     2161 acgcgggaga agctggcgga tgtggccagt gtggtggaac tggtttatgc agccggcata
     2221 gccgccagcg ttaaatccag gaaagcacct tccggcaccc aaattcccaa tattatttac
     2281 tgcaacgtgg gcagacggca tgccggacac aatgtctatc atgagtatga tatcctctgt
     2341 gacatcgccg gcgggatgcc ggccacattg ccgctgtcca aggaatacaa cagccccatc
     2401 gtgggtgact ttgtcagaaa gtatacgcgg cggcgggaag gggtatcaga tgaaaaccat
     2461 tatcgcgcct accatttggc cagcgatatt ctgaacggtg aaatgggtgc tgccttgttg
     2521 gtggctggtg ttcacggtgg cggttctccc atcatggagg acatcgcgat tctgggttcg
     2581 tatgataccg aagggaaaaa gaatctggcc cgacatctgg ccggcatcaa agagtaacca
     2641 gagcaggcca acgatgcagt cagaaacgtc cggcatagtg cgggacgttt ctttaaggaa
     2701 cgacacgatt atgctgcggt acagtcggag ttaatatttt taaagtaaag ggaggggtcg
     2761 aattgtgata acatctcagc aatatctaga cagaatcgcc aaaatgagac ccaatatcta
     2821 tgtggatgga gaagttatcg ggcgggatca tccgctggtg ctgaaagcca gccagatcat
     2881 tcaatcaacc tttgatctgg cggaagatcc tgaatacagt aaatggctca aaaccacctc
     2941 gcatatcagc ggccaggaaa tcagtcgttt tacccacatt caccagtcgg tcgaagatct
     3001 gttgctgaaa caggaaatga cccgcaagtt gtgcaaccgc agcggcggtt gtgtatatcg
     3061 ctgcatgggc tgtgatgcta tgaatgcctt gtcggtaaca accaaaaaca ttgatttgaa
     3121 atacggcacc gattatcatg accggtttct caagtacctg gcatatttcc aggaaaacga
     3181 cctggtaggc tgtgctgccc aaaccgatgc caaaggcaac cgcagtttaa gaccagcgga
     3241 acagccggac ccggatgcgt atctgcatgt ggtggaaaga agagcagacg gggtagtggt
     3301 aagaggagcc aaactgcaca ataccatggc tccttatgcc gatgagatca tcgcattacc
     3361 aacccgtgcg ctgaaaaaga acgaagggga ttatgcgatt gcctttgcta ttcccgcgga
     3421 tacggaggga atttatctga tcggacgcaa ggcattctcc caatcgcggg aggaaggcct
     3481 ggaagcaccc tacaccaaac tgggttcgat cgaaagtttt accgtatttg ataatgtttt
     3541 tatccccaat gaacgcatct tcctggacgg tcaaaccgaa ttcggcagtg aactggcgat
     3601 gctgtttgcg ctctaccatc gtcacagtta ctgtggctgc aagcccgggg taggggacgt
     3661 gatgtatggg ctgaccgcat tactggccga ctacaacgga gtggaaaaga ccagtcatac
     3721 acgggagaaa ctggcagaga tcgccagcgt ggtggagctt gtcttcgcgg ctggtgtggc
     3781 tgccagtgta aagtccagaa aggcgccctc cgggacccag attcccaata gtatttattg
     3841 caacgtgggg cggcggcatg ccggacacaa tgtctatcac gagtatgata tcctctgtga
     3901 cattgccggc ggacttcctg ccacactgcc gctgtccaag gaatacaaca gcccggttgt
     3961 gggagatttc gtcaggaagt ataccaaacg ccgggaaggg gtttccgcgg aaaaccaata
     4021 ccgtgcctat aatatggcca gtgatatctt aaacggagat gtgggtgcgg ctttgctggt
     4081 ggccggtgta catggcggcg gatcaccgat tatggaagac atcgcgattt tgggcacgta
     4141 tgataccgaa gggaaaaaga agctggc
//
LOCUS       DAOTHZ010000126         4163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_25364_length_4163_cov_6.440847, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000126 DAOTHZ010000000
VERSION     DAOTHZ010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25364_length_4163_cov_6.440847"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2327)
                     /locus_tag="P5549_10055"
     CDS             complement(<1..2327)
                     /locus_tag="P5549_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13455.1"
                     /translation="MSSISRLLSLEPGDRHRLQLMIPVFFSLGMGEVLGISSATSIFN
                     IRYGVEHLPLMYVLEALGLLLTSLAIADMSGRMNRARFLRVSYGIMAGLVLINGLILL
                     SARIAAGALGAFFYPFLLVSSMVIFFQLTPLIWLVAVDICPTQQAKRLFPLLSGSSTI
                     GCIVAGFVAKTLAPAGVEIIYFLWAFFLLGGGYFLSQTIQYYIVPVNLPAEEASADLK
                     DSISSVVSSRFLRGMLGLLTFIMILNFVMDYQFNTTARLFYSNEADLAGFLAIFLAVS
                     NMVAVIIELGFLSRIMAALGVGNILLLVSVGLGVCFLLMVFLGTGPMALGTVFIGYLL
                     TKIMVNVLGEPSYQLLFKVVPAKERDGVRFLVEALFILGGMLGGAALAALHSQGWLSL
                     QAVSGLALLLAGLTIYTAWQARAMYLHELIKAIGQGVEDLKENGASLLGNLVPAFFLT
                     RLLTFLHHPDERKRSLAMEIIGQIDPARLSPWINELLQDPFDEVRIRALAYCLHLEPQ
                     HYRQDLVHGACLDNNPEIRAAATRLFTSPSNGHTWLESALQDNSPLVVGQAVITLSAH
                     QTPKENPGIRTAVQRCLEASPADAAAICTAIGEAGLDEFAPQLQACLDREPILKAAAC
                     EALGKLQFLDSIPTMIAVYASSDLEFQQIADQALINMGEEAVTVLLQELEQSRDLRSW
                     LAIIKAISARPLPANASALLIDSCLDQLHQFAHDKRLPAQLQASGLTALADLAELRCQ
                     EIHALLLEAGWAVMGGLYDHYVITRIKAASQETDR"
     gene            complement(2365..3849)
                     /locus_tag="P5549_10060"
     CDS             complement(2365..3849)
                     /locus_tag="P5549_10060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRW13456.1"
                     /translation="MGKYDECLSLTIPNHMRYLPLILDSTGAYARMLGLDDKQIRDVT
                     LGVEEAVVNVLEHAFDEGEQASFELVFQQVPLGMQILIRDQGMPFDPQELAKMADAAF
                     FGEDTRGLGLYLMYRFMDEVSFVNLGKNGKETRLLKYLGRIPEAFVASPSEAEHPQEN
                     IPSYQIRRMLPHEAVEVSKCAYMSYGYSYANEHVYYPERLRQLNEEGKMVSFVAVTDN
                     GEIIGHVALIFDDSDPLVPVSDDAFVKPQYRGAGCLNDLGRALVQWATDHAILGSYAL
                     AVTSHPYSQKVASRWGYHDTAIYISIDTPFDFKAIKEEAGQRESETLLFRYFRPSRNV
                     NIYAPARHAGMITQIYQHQGVNTTVLSPAPDLQLPAEEAVIEVKYDPSIIAYIKVKQY
                     GHQITSEINRILKGLCIQRTETIYLYLPLNNPFTSLLTSDFEALGFFFCGIMPGSEGR
                     DQLILQYLNNQIIDYRQLCMHSQMGQKLLAYIQQHDPNQSLQAE"
BASE COUNT         1094 a         1079 c         1099 g          891 t
ORIGIN      
        1 tcccgatcag tttcctggct ggcggccttg atccgagtga tgacatagtg gtcatacaag
       61 ccgcccatca ctgcccagcc agcttccagc agcagggcat gaatttcctg acaacgcagt
      121 tcagccagat cagccagggc ggtcagaccg cttgcctgca gttgggctgg caggcgttta
      181 tcgtgcgcaa actggtgcaa ttggtccaga cagctgtcga tcagcagggc actagcgttg
      241 gctggcaagg gacgagcgga tatagccttg ataatggcca accaggaacg caagtcgcga
      301 ctttgttcca attcctgcaa aaggacggta acggcctctt ctcccatatt gatgagtgcc
      361 tgatcggcaa tttgctgaaa ttccaggtca gagcttgcgt agacggcgat cattgtcgga
      421 atggaatcca ggaattgcag ttttcccaag gcctcgcagg cagccgcctt gagaatgggt
      481 tcgcggtcca ggcaagcctg taattgcggg gcgaattcgt ccagtccggc ttcgccgata
      541 gcggtacaga tggcagccgc atcagccgga gaagcttcca gacaacgttg tacggcagtg
      601 cggatgccag ggttttcttt cggagtttga tgggcggaca gggtgataac tgcctgccca
      661 acgaccaaag gactgttatc ctgcagggct gattccagcc aggtatggcc gttggacgga
      721 gaagtaaaca gacgggtggc ggctgctcgt atttccggat tattgtccag acaggcgccg
      781 tgtaccagat cctgccggta atgttgcggt tccagatgta aacagtaggc cagagcgcga
      841 atacgcactt cgtcaaaggg gtcttgcagc agttcattga tccacggtga cagacgggcc
      901 gggtcaatct ggccgatgat ttccattgcc agactgcgtt tgcgctcatc ggggtgatgc
      961 aaaaaggtca acaggcgggt caggaaaaaa gcgggtacca aatttcccag cagggaggca
     1021 ccgttttctt ttaaatcctc cacaccttgg ccgatggctt tgatcaattc atgcagatac
     1081 atggcccggg cctgccaggc tgtatagata gtaaggccgg ccagcagcag ggccagcccg
     1141 gaaaccgcct gcaacgagag ccatccctgg gaatgcagcg ccgccagagc agcaccgccc
     1201 aacatgccgc ccaggataaa cagcgcttcc accagaaagc gtactccgtc ccgctctttg
     1261 gcagggacga ccttgaacag caattgatag gagggctcgc ccagcacatt gaccataatt
     1321 ttggtcagca aatagccgat aaaaacggtg cccagcgcca tcggaccggt tcccaggaaa
     1381 accatcagca aaaagcaaac tcccaggcca acactgacca gcaagaggat attgccgacg
     1441 cccaaagctg ccataatgcg gctcaggaag ccgagctcga tgattaccgc caccatattg
     1501 gacaccgcca gaaaaatcgc cagaaaaccg gcgagatcag cttcgttgct gtaaaacaga
     1561 cgggccgtgg tattgaactg ataatccatc acaaagttca gaatcataat gaaggtcagc
     1621 agacccagca tgccccgcag gaaccgggaa gaaacaacgc tgctgatact gtcctttaaa
     1681 tcagcacttg cctcttcggc aggcaggttg actgggacga tatagtattg aatggtctgg
     1741 cttaaaaaat acccgccccc cagcaagaag aaggcccaca gaaaataaat gatttcaacc
     1801 cctgctggag ccagggtctt ggcaacgaat ccggcaacaa tgcagccgat tgtacttgag
     1861 ccggacagga gagggaagag gcgcttggcc tgttgggttg ggcagatatc cactgccacc
     1921 agccagatca ggggcgtcaa ctgaaaaaag atgaccatcg agctgaccag caggaaagga
     1981 tagaaaaacg ctcccaaagc gcctgcggca atcctggctg acaacaaaat caaaccattg
     2041 attaacacca agccggccat gataccatag gacacccgca aaaagcgggc ccggttcatg
     2101 cggcctgaca tgtcggcaat ggccagggag gttaataaaa gaccgagcgc ctccagcaca
     2161 tacatcagcg gcaggtgttc caccccgtag cggatattaa agatagaggt cgcggagctg
     2221 atgcccagca cttctcccat gcccagagag aaaaaaaccg gaatcatcag ctgcaaacgg
     2281 tgccggtctc ccggctccag tgacagcaaa cggctgattg acgacaaaca caacccatcc
     2341 tttcccggca gcttgatgcc tgtattactc tgcttgcagc gattgattgg gatcatgctg
     2401 ctggatataa gccagcagct tttgtcccat ctgcgaatgc atgcaaagct ggcggtagtc
     2461 gatgatttga ttgtttagat actggaggat aagctgatcc cgtccctctg atcccggcat
     2521 gatgccgcag aagaaaaagc ccaacgcttc aaaatccgag gtcagcaggg aggtaaacgg
     2581 gttgttcagg ggaagataca ggtagatggt ttccgttctc tggatacaca gaccctttaa
     2641 gatgcggttt atctcactgg ttatctggtg gccgtattgt ttcactttta tataggcaat
     2701 gatactggga tcatatttaa cttcgataac agcttcctcg gcaggcagtt gcagatcagg
     2761 ggcaggggac aagacagtgg tatttacccc ttgatgctgg tagatttgag taatcatccc
     2821 ggcatgccgg gcaggcgcat aaatgttcac attccgggat ggacggaaat agcggaacaa
     2881 aagcgtttcg ctttcacgct gaccggcttc ttctttgatc gctttgaaat caaacggcgt
     2941 atcgattgaa atatagatgg cggtatcatg gtatccccat cttgaagcga ccttttggga
     3001 gtaggggtga ctggtcaccg ccagggcata actgcccaga atagcgtgat cggtagccca
     3061 ttgcaccagc gcccttccca aatcgttcaa acagccggcc ccccggtact ggggcttgac
     3121 aaacgcgtcg tcggagactg gcaccaacgg gtcgctgtca tcaaagatca gggccacatg
     3181 gccgataatc tctccgttgt cagtgacggc aacaaaggac accattttgc cttcttcatt
     3241 cagttggcgc aaacgttccg gatagtatac atgttcattg gcataggaat agccgtagga
     3301 catataggca cacttcgaaa cttccaccgc ttcatggggg agcatgcggc gaatctgata
     3361 agacgggata ttttcctgtg ggtgttcggc ttcagaggga gaagcaacga acgcttccgg
     3421 gattctgccc aagtatttca acaggcgggt ttccttgccg ttcttgccca ggttgacaaa
     3481 ggagacttca tccatgaaac ggtacatcag atacaatccc aacccgcggg tatcctcgcc
     3541 aaaaaaagcc gcgtcagcca ttttggccag ttcctgaggg tcaaaaggca ttccctgatc
     3601 gcggatcagg atttgcatgc ccaacggtac ttgctgaaaa accaattcga agctggcttg
     3661 ttcgccttca tcaaaagcat gttccaggac attgactacc gcttcttcca cgcccaaggt
     3721 gacatcacgg atctgcttgt catccaaccc caacatccgg gcataagctc ccgtgctatc
     3781 gagaataagc ggcagataac gcatatggtt gggaatggtc aggctgagac actcgtcata
     3841 tttacccaaa cagaaatcct ccctcaaagc aagtattttt cagagatgct aacacatttg
     3901 acagcgtcga tattatttcc ttcaaaaaat acaaaatatt taaaggatta ttcacttgcg
     3961 tgtcaaaata aaataataat tttaatgatg gtgagtttgg gggacaggca aatggagtac
     4021 cgcatcaaga acaatctggc cgaaatccag ctgctggccg aagcggttga ggcttttgga
     4081 gaagaactcg ggttgctgcc caaaactgta tttcaggtta acctggcgct ggaggaactg
     4141 ctgaccaata ccatcaacta tgg
//
LOCUS       DAOTHZ010000127         4145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_25483_length_4145_cov_6.525183, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000127 DAOTHZ010000000
VERSION     DAOTHZ010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25483_length_4145_cov_6.525183"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="P5549_10065"
     CDS             complement(<1..1256)
                     /locus_tag="P5549_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRW13457.1"
                     /translation="MINSWKFSSGAKLVVEEIPYLKSAAVGVYIKVGSRNETPEIAGA
                     SHFIEHMLFKGTSKHSARQIAEAFEGMGGQLNAFTSKEYTGLYARTLDEDIFQAMEII
                     FDMIFDSKFAKKDFNTERGVIIEEINMYEDAPDDLIHDVFAQKFFAGHPLGFPILGTL
                     ETIHNMERDTLRDYYHTHYQPDNIIFSVAGNVEANKIRDFIEKKCEGHSNHPLPERIE
                     SPHPGSRFIEMVSKEVEQVQICLGVPGISYHSEERYTQNVLNSILGGGMSSRLFQSLR
                     EERGLAYSVYSYPSSYRDSGLYTIYIGTSPGKVPEFFAALNEQLQDLLSNGVSAEEIE
                     RTQKLMKSSIYLSMESVMNRMSRLAKSMLMYDDIQPAEEVIEKIYAVTPEMVNHYAAR
                     KLAPETFSLGAIGNQEALPAVEKEFQ"
     gene            complement(1310..2464)
                     /locus_tag="P5549_10070"
     CDS             complement(1310..2464)
                     /locus_tag="P5549_10070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRW13458.1"
                     /translation="MKKNKFAIRVQVLGLALVLTLLSGFLFTAPALAAPTIHSKYYCL
                     VDGDTCQPIVSKNADHPRPIASTTKIMTAILAVEYAAMDEIATVSPYADHTAEYTIGL
                     RAGQQLPLGELMKATLIKSSNDAAVVLAEHIAGDEALFAHMMSLKAFAIGAMHTHFVN
                     ASGLPADDAFSTAYDLAVMGRYALSKPLVARLVAMPQAEFKHPAYREPLTIRNTNGLI
                     TSYSGITGIKTGTANESGKCLVASATRQKRNLIAVVLKSGDRTGDCARLLNYGFNQSR
                     SIKVIDSHEVFKNAKINKAVQPYVEIVPSADLWLWVGESPPDIQKRVRMNYLLEAPLP
                     RAYKVGEMDIYTEGQYVETIELRTRENVSRETFWGQKILQDLWQKLKNQE"
     gene            complement(2451..3209)
                     /locus_tag="P5549_10075"
     CDS             complement(2451..3209)
                     /locus_tag="P5549_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRW13459.1"
                     /translation="MKIYYVPKKNMIIVAAALLLALAMLGWGMIDRQPAMTTSEVNEP
                     IFQGNSGAKAVALTVNVDWGEEYIPAMLKQFKAADAKVTFFVTGRWAEKNPALLKQMA
                     QAGHSIQNHGYRHLHFNSLSPDQGSAEIKKAEEVIRGITGTKTRYFASPYGEFNANVV
                     AAAHNIDYKLIMWSVDTVDWKRPLPETIVSRVMKKAHNDAIILMHPTEPTLKALPEML
                     RQLQAEKYQLLPIDQVVLDAPSKPEQGGKANEKK"
     gene            complement(3206..4009)
                     /locus_tag="P5549_10080"
     CDS             complement(3206..4009)
                     /locus_tag="P5549_10080"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRW13460.1"
                     /translation="MLTFFISLSLLGSWLDKIERHCHGTKAGVRIEEIEAGRLLPAEA
                     YEIVAELAVRCQKLPVEPTLDKDSGAIIDGRPGIIVDIDQTVANALSAAPATTIKLVV
                     RPLLPRYSKSSLEQARYCIGRYQTGFSGTQARYKNIRMACRSVNHTIVWSGQEFSFND
                     VTGPRTPERGYLPAPIILDGDFDIGHGGGVCQVSSTLYNAARQARLHITERHAHSKPV
                     HYVPSGQDAAVSFGDQDLRFVNPHPGPVIVKMHLGQGTVSAEIWGGEYP"
BASE COUNT          871 a         1026 c         1156 g         1092 t
ORIGIN      
        1 ttttggaact ctttttccac ggcgggcagg gcttcctggt tgccgatggc gccgagggag
       61 aaggtttccg gggcaagctt gcgggcggcg tagtggttga ccatttccgg ggttacggca
      121 tagatttttt cgatgacttc ttcagctggc tggatgtcat cgtacatgag catggatttg
      181 gccagacggc tcatgcggtt catgacgctt tccatgctca aataaatact ggatttcatg
      241 agcttttgag tgcgttcgat ctcttcggcg ctgacgccgt tgctgagcag atcttgcaat
      301 tgttcgttaa gtgctgcgaa aaattcaggt actttgcctg ggctggtacc gatgtaaatg
      361 gtgtagaggc cgctgtcccg gtaactggac ggataactgt aaacggagta ggccagcccg
      421 cgttcttcgc gcaggctttg gaacagccgt gaactcatgc cgccgccgag gatgctgttg
      481 agcacgttct gggtatagcg ttcttcgctg tgataggaga tgccgggaac gcccaggcag
      541 atttgcacct gctcgacttc tttggacacc atttcaataa accggctgcc gggatggggg
      601 ctttctatcc gttccggcaa ggggtggttg gaatgcccct cgcatttttt ctcgataaaa
      661 tctcgaatct tgttggcctc aacgttgccg gccacggaaa atatgatgtt gtcgggttga
      721 taatgggtat gataatagtc gcgcagcgta tcgcgctcca tattatggat ggtttccagg
      781 gttcccaaaa tgggaaagcc cagtgggtga ccggcgaaaa atttctgggc gaaaacatcg
      841 tgaatgagat catcgggtgc gtcttcatac atgttgattt cctcaataat cacgccgcgt
      901 tcggtattga aatctttttt ggcaaatttg gaatcgaaaa tcatatcgaa gataatttcc
      961 atggcctgaa atatgtcctc atccagggtg cgggcataca ggccggtgta ttccttggaa
     1021 gtaaaggcgt tgagctgtcc gcccatgcct tcaaatgctt cggcgatttg acgggcactg
     1081 tgcttgctgg tgcctttgaa cagcatatgt tcaataaaat ggctggcccc ggcaatctcc
     1141 ggggtttcgt tgcgcgatcc gactttaatg tatacaccta cggccgctga tttgagatac
     1201 ggtatttctt ccaccaccag tttggcgccg ctgctgaatt tccagctgtt tatcatgttt
     1261 gtttcctcct gtattccatc ttgtttagtt tcattcttcc ggggaccgct tattcctgat
     1321 tttttaactt ttgccagagg tcctgtaata ttttttggcc ccaaaatgtt tcacgtgaaa
     1381 cattttcccg ggttcgcagt tcaatggttt ccacatactg cccttctgta taaatgtcca
     1441 tttcccccac cttataggcc ctgggcagcg gggcctcgag cagataattc atgcgtactc
     1501 ttttttgaat gtcgggcgga ctttctccca cccacagcca caggtcggcg gacggcacga
     1561 tctcgacata gggttggaca gctttgttta ttttagcatt tttaaatact tcatgggaat
     1621 ctatcacttt gatgctccgg ctttgattga agccataatt gagcaggcgg gcgcaatcgc
     1681 cggttcgatc gccggatttg agcactaccg caatgagatt tcgcttctgg cgggtggcgg
     1741 aggcgacgag gcatttgcct gattcattgg cggtgccggt tttgatacca gtgattcccg
     1801 aatagctggt gataagacca ttggtattgc ggatggtcag gggttcacgg taggcgggat
     1861 gtttgaattc ggcctggggc atggctacca ggcgggccac caaaggcttg gaaagggcat
     1921 aacgacccat gacggccagg tcataggcgg tgctgaaggc gtcatcggcc ggcagcccgg
     1981 aggcattgac aaaatgggtg tgcatggcgc caatggcaaa ggctttcagc gacatcatat
     2041 gggcaaacag ggcttcatca ccggcgatgt gttcggccaa aacaactgcc gcatcgttgg
     2101 aggatttgat cagggtggct ttcatcaatt ctcccagcgg cagctgttgc ccggcgcgca
     2161 ggccgatggt atactcggcg gtatgatctg cataagggct gaccgtggcg atctcgtcca
     2221 tggcagcgta ctccacagcc aggattgcgg tcatgatttt ggtggtgctg gcaatggggc
     2281 ggggatgatc cgcatttttg ctgacgatgg gctgacaggt gtcaccgtcc accagacagt
     2341 aatacttgga atggatggtt ggtgcggcca gggctggggc agtaaacaag aaccctgaca
     2401 gcagcgtcag gacaagtgcc agccccaata cctggacgcg aatcgcaaac ttattttttt
     2461 tcattggctt tcccaccttg ttccggcttg gacggcgcat ccaacacgac ctggtcaatg
     2521 ggcagcagtt ggtatttttc cgcctgcaat tgccggagca tttccggcag ggctttgagc
     2581 gttggttccg tgggatgcat gagaatgatg gcatcattgt gtgctttttt catcacccgg
     2641 ctgactatgg tctcgggcaa aggacgtttc cagtcaactg tatcaacgct ccacatgatg
     2701 agtttataat cgatgttatg ggctgcggcg accacattgg cattaaattc tccataaggc
     2761 gaagcaaaat agcgggtttt ggtcccggtg atgccgcgga tgacttcttc ggcttttttg
     2821 atctcggcgc tgccctgatc cggggacaaa ctattaaaat gcagatggcg gtaaccatgg
     2881 ttctgaatgc tgtgaccggc ctgggccatt tgcttgagca gcgctggatt cttctccgcc
     2941 cagcgtccgg tgacaaaaaa ggtaaccttg gcatcggctg ctttgaattg tttgagcatg
     3001 gcggggatgt attcttctcc ccaatcaaca ttgacggtca gggcaacggc cttggctccg
     3061 ctgttgcctt gaaatatcgg ttcattgact tcggaagtgg tcatcgccgg ctgccggtcg
     3121 atcatccccc agcccagcat ggccagggcc agcagcaagg cagcagcaac gatgatcata
     3181 ttttttttgg gaacataata aattttcatg gatactctcc tccccatatt tctgcgctga
     3241 ccgttccctg tcccaggtgc atcttgacga taacggggcc ggggtgcggg ttgacaaagc
     3301 gcaaatcctg atcgccaaaa ctgaccgcgg catcctgccc ggagggtacg taatgaaccg
     3361 gtttgctgtg cgcgtgccgc tcggtgatgt gcaaacgggc ttgccgggcg gcgttgtaca
     3421 aggtactgga tacctggcag acacctcccc catgaccgat atcaaaatca ccgtccaaaa
     3481 taatgggggc gggcagatac cctcgttcag gggtgcgcgg tccggtcaca tcgttaaagg
     3541 aaaactcctg tccggaccag acaatggtat ggttgacgct gcggcaggcc atgcggatat
     3601 ttttatagcg ggcctgggta ccgctgaaac cggtctgata gcggccgatg cagtagcggg
     3661 cctgctccaa actacttttg ctgtaacggg gcagcaacgg cctcaccacc agttttatgg
     3721 tggtcgcggg cgcggcggaa agggcattgg caaccgtttg atcgatgtcg acaataatcc
     3781 ccgggcgtcc gtcgatgatc gcgccgctgt ctttatctag cgtgggttcg acgggcagct
     3841 tctgacagcg tacggccagt tcggcaacaa tttcgtacgc ttctgcaggc agcaggcggc
     3901 cggcctcaat ctcctcaata cgaacgcctg ctttggtgcc gtggcagtga cgttcgattt
     3961 tatcgagcca actgccgagc agactaagac tgataaaaaa ggtcagcaaa aaaagggctc
     4021 tgccgtccaa tttgactaca tataaaccct tcttaaaaaa ataataccca ccgcctttgt
     4081 tttgctttta aagcatattc ggcagtgagc actactattc agtttttttc tttttggctt
     4141 cgcca
//
LOCUS       DAOTHZ010000128         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_25655_length_4124_cov_5.716146, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000128 DAOTHZ010000000
VERSION     DAOTHZ010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25655_length_4124_cov_5.716146"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="P5549_10085"
     CDS             <1..226
                     /locus_tag="P5549_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13461.1"
                     /translation="APALQAGCRRFEPVIAHHLSTRISVTRKSFFCGQVQAGQSNQQD
                     IPNQKDWTLKYLYTEFAEGGGMVAGNQRNN"
     gene            269..1045
                     /locus_tag="P5549_10090"
     CDS             269..1045
                     /locus_tag="P5549_10090"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HRW13462.1"
                     /translation="MDYVSAGRFTNEKARQMIPILRQYIPHIHWRSEWSEGELGDAMP
                     YSDIYVPEYQLHEAEHVIGLLENGVDLSNYRSNSSYPDLFEEINGSLQVVKPLAAFAE
                     HVRELINLAEDFSSEDMVGYLRRISHQLIRIYALQSELPDCSGSYYYQPWLNFSLPHK
                     LGPFSLYYDVGNPFHQQVSPQSLEKTLEHILEVLHAGIIHYTMYHETGNYKYLSLAAN
                     VWKNQFFGAEGWGTAVVNVLKVFHHAIMYLQQGKLPDQDF"
     gene            1061..1780
                     /locus_tag="P5549_10095"
     CDS             1061..1780
                     /locus_tag="P5549_10095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRW13463.1"
                     /translation="MKRKLALPHSLDLIEYEIVYSRRRTMAIKIDPEGNVKVYAPQGT
                     SDQAVGKMVESKAAWLCRNLAEIRSANAGAVSRHFTEGETFPFLGRDYPLQVKVKPAA
                     QRITVTLEEDKFEVMTPVADEDIVRGAMEAWYRRSATEYINTRIHHFQSLLPVAPVRV
                     TIRNQKTRWGSCSNRGSLNFNWRLMMAPAEVVDYVVVHELCHLLHPNHSKNFWNQVSA
                     VLPDYRARQDWLKKNGVRLNF"
     gene            1903..2340
                     /locus_tag="P5549_10100"
     CDS             1903..2340
                     /locus_tag="P5549_10100"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRW13464.1"
                     /translation="MARRSRPSDDWQFLRNVGTHYELELITSILAQDGILVMKKSQGS
                     GAYTDVIMGTSLTGYDIYVPARRLAEAQDILENLPVFEENEINYQERVQDAEWALPNI
                     AAEPIEEGFLLRNRGYLKILFFFFVVLPLIFGLLVALFKNLRP"
     gene            complement(2429..2992)
                     /locus_tag="P5549_10105"
     CDS             complement(2429..2992)
                     /locus_tag="P5549_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13465.1"
                     /translation="MAVKFSIPGLLTTLVLGLIILAQPYGAELVPAGAEDSRSKLEKQ
                     VFSETIEDFEFYTALISDVQYRRDCVYPVAVKAADQKEAAEWLRQGFCDELAHSLACF
                     YLAWDEALQRLVVIPADGIPVLTRQDWPQTVITFRNEHQAILECLYDNCYAPGDRYCY
                     QIEVEKYEERWKIASLTLHQIQPGQEF"
     gene            3314..>4124
                     /locus_tag="P5549_10110"
     CDS             3314..>4124
                     /locus_tag="P5549_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW13466.1"
                     /translation="MDQGLAMFERFQEQVHTIPRLFYNTVENFGERPALKYRVGEDYM
                     ILDYNGFAEIVEKMGCGLMELGLNKADRVCLMAHTSAPWGWADFAIQTAGGVTVTVYP
                     SLSATETAFIINHSEARYLFAGNASIAEKVISVWEQVPTMEKVIVLESGYVSSDERII
                     SIDRLRELGKQLLAKDWGIYKRTWEALTGDDPSSIIYTSGTTGDLKGSLLSQQDLVGA
                     LSRSLKHMTLGGYSASYNDVAFSLLPLSHIWERNNSYLAMIVVGGCIGYAQK"
BASE COUNT         1100 a          941 c         1042 g         1041 t
ORIGIN      
        1 agcgcctgcc ttacaagcag gatgtcggcg gttcgagccc gtcatcgccc accatctgag
       61 cacaaggatt tccgtgacgc ggaaatcctt tttttgtggt caggtgcagg caggacaaag
      121 taaccagcaa gatattccca accaaaaaga ttggacgttg aaatatttgt atactgaatt
      181 tgcagaaggc gggggcatgg ttgcaggaaa tcaacgaaat aactagaaat aaataatagt
      241 gattaccatc aagacaaagg ggaagaatat ggattacgtg agcgctggca ggtttaccaa
      301 tgaaaaagcc agacaaatga taccgatatt gcggcaatat atcccgcaca ttcattggcg
      361 ctcggagtgg agcgaggggg agttgggcga tgccatgcct tattctgata tttatgtgcc
      421 ggagtatcag cttcatgagg cagaacatgt cattggcctg ttggaaaacg gagtcgacct
      481 gtccaactat cgctccaaca gcagctaccc cgatctgttt gaggaaatta acggcagttt
      541 gcaggtggta aaacctttgg cggcttttgc cgagcatgtc agggaactga tcaatctggc
      601 ggaagacttc agcagtgaag acatggtcgg ctatttacgc cgtatctccc atcagttgat
      661 tcgaatttat gcgttgcagt ctgaattgcc agattgttcg ggcagctatt attaccaacc
      721 ctggcttaat tttagcttgc cgcataagct gggacccttt tcgctttatt atgatgtcgg
      781 taatcctttt catcagcaag tatctcccca gtccctggaa aaaaccctgg aacatattct
      841 ggaggtcctg catgctggca ttattcacta tacgatgtat catgaaacgg ggaattacaa
      901 atatctttcc ctggcagcca acgtttggaa aaaccagttc ttcggcgcag aaggctgggg
      961 aacggcagtg gtcaacgtgc tgaaggtttt tcatcatgcg atcatgtatt tacaacaggg
     1021 caagctgcct gatcaggatt tttaaggaga acacgattac atgaaaagaa aactggcgct
     1081 tcctcacagt cttgatctga ttgagtacga aattgtttac agccggcgcc ggacaatggc
     1141 tattaaaatt gacccggaag gcaatgtcaa ggtttatgcc ccgcaaggta ccagtgacca
     1201 ggcggttggc aaaatggttg agagcaaggc ggcctggctc tgccgcaacc tggctgagat
     1261 ccgttcagcc aatgcaggtg cagtaagcag gcatttcacg gagggggaaa cgtttccatt
     1321 tttaggccgc gattatccgt tgcaggtaaa ggtaaagcca gcggcgcaaa gaattaccgt
     1381 aacattggaa gaagacaaat ttgaggtcat gacgccggtg gccgatgaag atattgtccg
     1441 aggcgctatg gaagcttggt accgcaggag tgcgaccgaa tatatcaaca cccggattca
     1501 tcattttcag tccctgttgc cggtcgcccc tgtccgggtc acgattcgca accagaagac
     1561 ccgatggggc agctgcagca accggggcag cctgaatttc aactggcggt tgatgatggc
     1621 gccggcagaa gtggtggatt atgttgttgt tcacgaactg tgccatctgc tccatcccaa
     1681 tcattccaaa aatttctgga accaggtgtc agccgtgctg ccggattacc gggcacgtca
     1741 ggactggctg aaaaaaaacg gtgtccgact gaatttttaa tgagaaattt ttattaaaca
     1801 attttttatc gggcgggata aattatcaaa tgtggtcgta aaataaatta agcatggtag
     1861 tatatatata aagagagata aaatctataa agaggtgatc gtgtggccag aaggagcaga
     1921 cccagtgacg attggcaatt cttgcgcaat gtcggtaccc actatgaact ggagctgatc
     1981 acatcaatcc tggcccagga cggcattctg gttatgaaaa aaagccaggg atcgggtgcc
     2041 tatacggatg ttatcatggg cacttccctg accggttatg atatttatgt gccggccagg
     2101 cgcctggcgg aagcacagga tattttagaa aacttgccgg tattcgaaga aaatgaaatc
     2161 aattaccagg agagagttca ggatgcggag tgggctttgc ctaacattgc tgctgaaccg
     2221 attgaagagg gttttttgct gcgtaatcgt ggttatttaa aaattttgtt cttctttttt
     2281 gtcgtgctgc cgctgatttt tggtttgctg gtggccttgt ttaaaaacct ccggccttga
     2341 tgaaaaaccc cacctttttc ccggtaaact aatcgggccg ggcattgaca tgaatgattg
     2401 agcacagcaa caaacccttg ctgaggcctt aaaattcttg ccccggttga atctgatgca
     2461 gggtgagtga ggcaatcttc caacgctctt cgtatttttc cacctcgatt tgataacaat
     2521 aacgatcccc cggtgcgtaa caattgtcgt acagacattc cagtatggcc tgatgctcgt
     2581 tgcgaaatgt aataacggtt tggggccaat cctgtctggt caaaaccggg ataccatccg
     2641 ccggaattac caccagccgc tgcagcgctt catcccaagc caaataaaag caggctaggg
     2701 aatgcgccag ctcatcgcag aaaccttgcc gtagccattc tgccgcttcc ttctgatcag
     2761 ctgcctttac tgccaccgga tacacacaat cacggcggta ctgcacatcg ctgatcaacg
     2821 cggtgtagaa ctcaaaatct tcaattgttt cgctaaatac ctgtttctcc agcttgctgc
     2881 ggctgtcttc tgcacctgca gggacaagtt ccgccccata cggctgggcc aggattatca
     2941 ggcccagcac cagtgttgtc agcaagccgg ggatactaaa ttttactgcc attgttcctc
     3001 ctgcaagtca agtataatat gaattataac caaatggaaa aaatatacaa acgaaatatt
     3061 cccgcaatat acggtacaga agggatcatt cagacatatt ccgggaggca aaccgcttgg
     3121 aaaggaaaaa ttgagaattt acctcatgtc cttcacgatt cttcatactt tccgcaaatt
     3181 atcagtaaat aatgctgtca ttaggcatat atacgatata atatttatta tgaaacgtca
     3241 taaatatacc aggagaaagg aggaggcagc gcaaccgttg ttacttaact ttaaaacttg
     3301 gggggattat tgaatggatc aaggattggc tatgtttgaa cgttttcagg aacaggtaca
     3361 cacgattccc agattgttct acaacacggt tgaaaatttc ggcgaacgtc cggctttaaa
     3421 gtaccgggta ggcgaagatt acatgatcct ggactacaac ggctttgccg aaatcgttga
     3481 gaagatgggc tgcggcctca tggaactggg cctcaacaag gccgaccggg tttgtctgat
     3541 ggctcatacc agcgctccct ggggttgggc ggattttgcc atacaaaccg cgggtggtgt
     3601 gaccgttacc gtctatccca gtctgtccgc aacggaaacc gcctttatta tcaaccattc
     3661 ggaagcccgt tacttgtttg ccggtaatgc cagtattgct gaaaaagtca tttccgtctg
     3721 ggagcaggtt ccgaccatgg aaaaggtgat tgttttggaa tccggctatg tcagcagcga
     3781 tgaacgcatt ataagtatcg atcggctgcg tgaactgggc aaacagctgc tggccaaaga
     3841 ttggggaatt tacaaacgca cctgggaagc cctcaccggc gatgatccct ccagtatcat
     3901 ctatacttcc gggaccaccg gagacctgaa gggcagccta ttgagccagc aggatctggt
     3961 cggcgccctg agcagaagcc tgaaacacat gaccctgggt ggttacagtg ccagttacaa
     4021 tgatgtggcg ttttccctat tgccgctgtc gcatatctgg gagcgcaata attcctatct
     4081 ggccatgatt gtcgtaggag gatgcatcgg gtacgcccag aagc
//
LOCUS       DAOTHZ010000129         4110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_25769_length_4110_cov_6.411097, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000129 DAOTHZ010000000
VERSION     DAOTHZ010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25769_length_4110_cov_6.411097"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1106)
                     /gene="dapA"
                     /locus_tag="P5549_10115"
     CDS             complement(228..1106)
                     /gene="dapA"
                     /locus_tag="P5549_10115"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRW13467.1"
                     /translation="MAKMKLITAMVTPYNEKLEVDYEKAAELARYLIAHGSDGLVVSG
                     TTGESPVLSRDEKVTLFAVVKNAVGSNVQVWAGTGSYNTRESMELSQAAEIAGADGLL
                     LVNPYYSKPTQEGMFQHFRAIADSVSLPVMLYNIPGRTGVELLPDTIARLAETNNIVA
                     IKEASRNLDQVSQIKVMVGERLAIFSGDDSMTLPMLAIGGSGVVSIASHIAGNEIKQM
                     LTAFEQGNSTQAMQMHQYLFPLFKGLFITTNPIPVKEALNLLGFKVGGFRLPLCAAGQ
                     QELDAIRQLLSHYRLL"
     gene            complement(1126..2355)
                     /gene="dapG"
                     /locus_tag="P5549_10120"
     CDS             complement(1126..2355)
                     /gene="dapG"
                     /locus_tag="P5549_10120"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRW13468.1"
                     /translation="MRIIVQKFGGTSLATPDLRARVCDIVAATRNEGISVVVVVSAMG
                     RKNDAYATDTFIQLLKEANPCPPSREMDILLSCGEIISGVLLTSQFCARTIPAVFLNG
                     EQAGIVTDGNYGNAHILYVDPQRIMKQLEDGKVVVVAGFQGAGENGELTTLGRGGSDT
                     TASALGVALNAECIDIFTDVEGVMTADPRIVKDARLLDAITYNEICQLAREGAKVVHP
                     RAIEIAMQKNIPLRVRCTFNDSMGTMVANQMPGTDNRIHIIADRMITGITYTSNIAQI
                     MINTFNLQEARGIELKVFKSLALADISVDFITVHPESIRFTVGIDQAEKAQEVLSNLD
                     IISQIERDCAKVAIVGAAMTGIPGVMSKVVEALTENEITILQSGDSYTNIWCLVKREH
                     MEKAVQALHDKFELGCK"
     gene            complement(2369..3385)
                     /locus_tag="P5549_10125"
     CDS             complement(2369..3385)
                     /locus_tag="P5549_10125"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRW13469.1"
                     /translation="MSAGVRLAVVGATGAVGQEMLKVLAERDFPIKELICLADPREAG
                     SKMEFRGETYTVQAAGAEAFQQSDMALFAVGTEISKSLAGMAVENNCVVIDNSYAFRM
                     DETVPLVVPEVNPDDVEWHRGIIANPNCSTIIMVVAINPIFKEAGLKRVVVSTYQAVS
                     GAGIAGLHELEEASRCYLQGQPFAPQVFQNPIAFNLIPHIDVFVEQGYTREEMKMVYE
                     TRKIMHAPELQIAATTVRVPVFRSHSESINLETERPITPEAARKILAAAPGIIVQDDP
                     YNNDYPMPLSSSNTDEVYVGRIRKDISVNNGLVLWVAADQIRKGAATNAIQIAEILIK
                     KNQF"
     gene            complement(3468..4064)
                     /locus_tag="P5549_10130"
     CDS             complement(3468..4064)
                     /locus_tag="P5549_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HRW13470.1"
                     /translation="MQHARLEGVKVGVVLTGSHCTIADTLPQIQYLKTEGADIYPILS
                     YSVNETDNRFYRVDDLKAALKQISSRPLINTIIGAEPIGPQKMLDIAVVLPATGNTIA
                     KLANGIVDTPALMAIKAHLRNQRPVVIAVSSNDALGINARNIGLLLNMKMIYFVPFRQ
                     DDPLNKPNSVVARLDLLLDTTVCALQGRQIQPIMLGPA"
BASE COUNT          977 a         1085 c          936 g         1112 t
ORIGIN      
        1 ccgcccaggg gaatgatctg gattcgtgcg tcgtctgaca ctcaaacacc tcctgttatt
       61 caatttgttt ttccaataca tttattatcc gctcacaaat gcatgactta ttatagctta
      121 tcaataatct tgatacaact taataaatag ttgttttttg acgaatgcaa aaaaataatg
      181 ggcacccccg tgcccaccgt catcaggtaa attttggcgg ctatcgttta aagcaaccgg
      241 taatggctta acaactgacg gatggcatcc agttcctgct ggccggcagc gcacaacggc
      301 aaccgaaaac cgcctacttt aaagcccagc agattaaggg cttccttgac cggtatggga
      361 ttggtcgtta taaacaagcc tttaaacagg ggaaaaagat actgatgcat ttgcatcgcc
      421 tgtgtgctgt ttccctgttc gaaagcagtc agcatctgtt tgatttcatt gccggcgata
      481 tgggaggcaa tgctcaccac tccgctgccg ccgattgcca gcataggcaa tgtcatggaa
      541 tcatcgccgg aaaaaatagc caaacgctcc cctaccatga ccttgatttg ggaaacctga
      601 tccagattgc ggctggcttc cttgatcgcc acaatgttgt tggtttctgc caggcgggca
      661 atcgtatcgg gcaataattc caccccggta cgtcccggaa tattatacag catgaccggc
      721 agggaaacgc tgtcggcaat ggctctaaaa tgttggaaca tgccctcctg ggtcggtttg
      781 ctgtaatagg gattgaccag cagcaagccg tctgctcctg ctatttcggc tgcctggctt
      841 aattccattg attcgcgggt gttgtaggac ccggttccag cccacacctg tacattggaa
      901 ccgactgcat ttttcaccac agcaaacaag gtaactttct cgtcccggct caataccgga
      961 gactcccctg tggttccgct gacaaccaga ccatcactgc cgtgggcaat caaatagcgc
     1021 gccaattcag cagctttttc atagtcgact tccagttttt cattataagg tgtgaccatt
     1081 gcggtaatca gtttcatttt tgccattcgg ttcaccgcct tgctcttatt tacaccccag
     1141 ttcaaacttg tcatgtaaag cctgtacagc cttttccata tgttcccgtt tgaccagaca
     1201 ccagatattg gtataggaat caccggactg taaaatggtg atctcatttt ctgtcagcgc
     1261 ttcaacgact ttggacataa ccccagggat cccggtcatg gctgctccaa cgatggctac
     1321 tttggcacaa tcgcgttcga tttgcgaaat gatgtccagg ttgctgagaa cctcctgggc
     1381 tttttctgcc tgatcaatac cgaccgtgaa gcggatgctt tccggatgga ccgtaataaa
     1441 atctacgctg atatccgcca aggcgagtga cttaaaaact ttcagctcaa ttccccgggc
     1501 ctcttgcaga ttaaaggtgt tgatcatgat ctgggcaatg ttggaagtat aagtgatgcc
     1561 ggtgatcatg cgatcggcaa taatgtggat acgattgtcg gtgccgggca tttgattggc
     1621 gaccatggtg cccatagaat cgttgaaggt acatcttacc cgcagcggta tgtttttttg
     1681 catggcgatc tcaatggctc ttggatgcac aaccttggct ccttcgcggg ccaactgaca
     1741 aatttcatta taggtaatgg catccagtaa acgggcatct tttacgattc ggggatcggc
     1801 ggtcatgact ccttcaacgt cagtgaagat atcaatgcac tccgcattca gagctacgcc
     1861 cagggcactg gccgttgtat cgctgccgcc tctgcccagc gtcgttaatt caccgttctc
     1921 tcctgcgccc tgaaaccctg ccactacaac caccttgcca tcttccagct gtttcataat
     1981 ccgctgggga tcgacatata ggatgtgggc attcccataa ttgccgtccg tgacaatccc
     2041 ggcttgttcg ccgttgagaa atactgccgg gatggttcgg gcacaaaact gactggtcag
     2101 cagtacgccg gaaattattt ccccgcagga cagcaagata tccatttccc gcgacggcgg
     2161 acatggattg gcctctttca acagttgaat aaaggtatcg gtagcatagg catcattttt
     2221 tctgcccatg gccgacacta ccaccactac gctgatgcct tcgttgcgtg tagctgccac
     2281 aatgtcacag acccgggcgc gtaaatccgg ggttgccagg gatgttcctc cgaacttttg
     2341 aacaatgatc ctcaagcttc acaccgtcct aaaattggtt tttctttatc aggatttcag
     2401 cgatttggat tgcgttggtg gcagctcctt ttctgatctg gtcagctgcc acccacaaga
     2461 ccagtccatt gttaacactg atgtcttttc taattctgcc cacatacacc tcatcggtat
     2521 tggaactgga caagggcatg ggatagtcgt tgttgtaagg atcgtcctgg acaatgatcc
     2581 caggagcagc ggccagaatc ttgcgcgccg cctccggggt gatgggcctt tctgtttcca
     2641 gattgataga ttccgaatga ctgcggaaaa ccggcactct tactgttgta gcagcaattt
     2701 gcaattccgg ggcgtgcatg attttgcggg tctcatagac catcttcatt tcttcccggg
     2761 tgtatccctg ttccacaaaa acatcgatgt gcggaattaa attaaacgct atgggattct
     2821 gaaacacttg cggcgcaaac ggttgcccct gcaaataaca gcgactggcc tcttccagct
     2881 catgcaaccc ggcaattccg gctccggaaa cagcctggta agtagagaca acaacccgtt
     2941 tcaggccggc ttctttaaag attggattga tcgccaccac catgatgata gtggaacagt
     3001 tgggattggc aatgatcccg cgatgccatt ccacatcgtc cggattgacc tccggcacaa
     3061 ccagcggcac cgtctcatcc attctgaaag catagctgtt gtcaatgacc acgcaattat
     3121 tttcaacggc catcccggcc agtgatttgc ttatttccgt tccgactgca aacaaagcca
     3181 tatcagattg ttgaaatgct tccgccccgg ctgcctgtac agtataggtt tctccgcgga
     3241 attccatttt actgcctgct tcccgcggat ctgccagaca gatcaattcc ttaatgggaa
     3301 aatcccgttc tgccagcacc ttcagcattt cctgccccac ggctccggtc gcgccaacga
     3361 cggccaatct tacccctgca ctcacttgta tacctcccgt tctgctgcat atactgattt
     3421 attacctatt gtattctacc acaatcattt caaattacta ctattgatta ggccggcccc
     3481 agcatgattg gctggatttg tctaccttgc agggcacata cggtcgtatc caacagcaag
     3541 tcgagtctgg ctacaactga attgggcttg ttgagcggat catcctgccg aaagggcaca
     3601 aaatagatca ttttcatgtt gagcaaaagt cctatattcc tcgcattgat ccccagcgca
     3661 tcattgctgg agacagcaat cactaccgga cgctgattgc gcaaatgagc tttgatcgcc
     3721 atcaaggcag gggtatcaac gatcccgttg gccagtttgg caatggtatt accggttgca
     3781 ggcaagacta ctgcaatatc cagcattttt tgcgggccga tcggttctgc accgattata
     3841 gtattgatca aaggacggga agatatttgt ttaagggctg cttttaaatc atccacccga
     3901 taaaaacggt tgtcagtttc gttcacagaa taagacagga tggggtaaat atctgccccc
     3961 tccgttttta aatattggat ttgcggcagg gtatcagcga ttgtacaatg ggaacccgtc
     4021 aaaacaactc cgactttcac tccttccaag cgggcatgtt gcacttttcc acctccttgc
     4081 taggcaaaca ttgaaacttg ttcttgtttc
//
LOCUS       DAOTHZ010000130         4103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_25837_length_4103_cov_7.121294, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000130 DAOTHZ010000000
VERSION     DAOTHZ010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25837_length_4103_cov_7.121294"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="P5549_10135"
     CDS             complement(<1..655)
                     /locus_tag="P5549_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13471.1"
                     /translation="MPAGKIIITPQFRGPDNSGNGGYTCGRLASYINGPAEVTLKLPP
                     PLNQALDVVQDPNGGAALMKGEQTIATAVPASIHLTIPDPPSYETALQAAGQHSMMEV
                     HTYPGCFVCGPARKKADGLCIFAGRVENQDYVAAPWIPEQCFADESGVIRDEIVWAAL
                     DCPGAWAILARKLRPILLGRMAVEIMKPVKAGQVLVVTGWQIAEEGRKIETGTALFDE
                     "
     gene            complement(770..2257)
                     /gene="abc-f"
                     /locus_tag="P5549_10140"
     CDS             complement(770..2257)
                     /gene="abc-f"
                     /locus_tag="P5549_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HRW13472.1"
                     /translation="MSLINVNHLTFGYDGGIENIFENVSFQIDTDWKLGFTGRNGRGK
                     TTFLNLLRGKFEYRGSISASVSFEYFPYEVQDEGMDTATVIRSMNDEYEDWQLLRELN
                     LLEVAHDVLDRPFATLSNGERTKVLLAALFLKGNRFLLIDEPTNHLDMNGRQMVSQYL
                     RGKQGFILVSHDRAFLDGCVDHILSINRTDIEIQKGNFSSWWHNKQLQDNLEMARNDT
                     LKKDIKRLEESARRAADWSERVEKSKKGQRVSGIKPDKGYIGHKSAKMMKRSQAIKSR
                     RQEAAEEKTGLLKNIEISDSLKIRPLQYFTDTLVTLEEISIIYGERPICEGISFRIQS
                     GERVALCGKNGSGKSSLARLILGEDIPYQGIIRRGSRLKISYVSQSTAHLSGNLTDYA
                     REYSIDQTLLRTILRKLDFSRTMFDMNMEDYSDGQKKKVMIARSLCESAHLYIWDEPL
                     NYIDVISRMQIEDLLRCHRPTLLFIEHDRAFCDNIATQQINTSGG"
     gene            complement(2792..3214)
                     /locus_tag="P5549_10145"
     CDS             complement(2792..3214)
                     /locus_tag="P5549_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13473.1"
                     /translation="MVGFMQTIPATELRKNFSSAIDTVVREKPVAFKRNRDNLMLLSN
                     AQVLSLLSDYSIKAVFQSEEDGSITATLDGFDLVINASDREEAITRLAEELVEYAMEY
                     YDQFQLYFNAINRKQHFPYVLRVLLAEDLNEVKALIHA"
     gene            complement(3297..3875)
                     /locus_tag="P5549_10150"
     CDS             complement(3297..3875)
                     /locus_tag="P5549_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRW13474.1"
                     /translation="MNHSSPINPLGAIVDVETTGFSPDRDEIVELCIILFCVNHHGVA
                     EIVDAYTGLREPVNGINAAAARVNGIGWADVCGRDLDHQRVNELIRRAEFFIAHNASF
                     DRGFVTRLYPQAAYKHWYCSMSGINWRGKGYPNRRLQDLLTWHGIRPDRQHRAEDDVR
                     ATLALLNHCQSSGRTYLAELLERRAMVKNWKK"
BASE COUNT          980 a         1097 c          929 g         1097 t
ORIGIN      
        1 tttcatcgaa gagggctgtt cctgtctcta ttttgcggcc ttcttcggca atctgccaac
       61 ccgttacaac caatacctgg ccggctttta ccggtttcat gatttctact gccatacgtc
      121 ccaacaggat tggtcttaat ttcctggcca ggatcgccca tgctccagga caatccaaag
      181 ctgcccagac gatctcatcc cgaatcactc cagattcatc cgcaaagcat tgctccggga
      241 tccagggcgc cgccacataa tcctggtttt ctacccttcc ggcaaatata catagtccgt
      301 cggctttttt tcgcgctggt ccgcagacaa aacaaccggg ataggtatgg acctccatca
      361 tggaatgttg gccagccgcc tgtaaggcag tttcatagga tggaggatca ggaatcgtaa
      421 gatgaatgga ggcaggcacg gctgttgcga tggtttgttc ccccttcatg agggcggctc
      481 ctccgttagg gtcctgcact acatccaagg cttgattcaa tgggggaggc agcttcaggg
      541 taacttcagc cggaccgttt atatagctgg ccaatcttcc gcaggtatag ccgccattac
      601 cggagttgtc cggaccgcga aattgtggcg tgataatgat tttcccagca ggcatggttg
      661 ctgtctcctt tcggttttac aacttcttac tcacatattt ctccagcagc agatgaattc
      721 ctgcctttgt taacaatatc aaggacagcc tctgggttcg acaatttact taaccgcctg
      781 acgtgttaat ttgttgggtg gcgatgttgt cgcaaaaggc tcggtcgtgt tcgataaaca
      841 aaagggtcgg tcgatggcag cggagcaaat cctcgatttg catacgggag atgacgtcaa
      901 tataattgag cggctcatcc cagatgtaca aatgggcgga ttcgcacaga ctgcgggcga
      961 tcatgacctt tttcttctgt ccgtcgctgt agtcctccat gttcatgtca aacatcgtcc
     1021 tggaaaagtc cagcttgcgt agaattgttc tcaaaagcgt ctggtcgatg ctgtattccc
     1081 tggcataatc ggtcaggttg ccgctcagat gcgccgtgga ttggctgaca taggaaatct
     1141 ttagtctgct cccccggcgg atgatgccct gataagggat gtcttcgccc aggatcagcc
     1201 gagccaggct tgatttgcca gagccgtttt tgccgcacaa ggcgacccgc tcaccggact
     1261 gaatacggaa actgatccct tcacatatgg ggcgttcccc atagatgata ctgatctctt
     1321 ccagcgtaac caaggtatcg gtaaagtact gcagcggccg gatctttaaa gaatcgctga
     1381 tttcaatgtt cttcagcagt ccggtttttt cctcggcagc ctcctggcgg cggcttttga
     1441 tggcctggga acgtttcatc attttggcgg atttgtgacc gatgtagcct ttgtcaggct
     1501 tgatgccaga gactcgctgc ccttttttgg acttttccac ccgctccgac caatccgcag
     1561 cgcgccgggc tgattcttcc agccttttga tgtccttttt caaggtatca ttacgggcca
     1621 tttccaagtt gtcctggagc tgcttgttgt gccaccagga agagaaattt cccttttgaa
     1681 tctcaatatc agtccggttg atggataaaa tgtgatccac acagccatct aaaaaggccc
     1741 gatcgtggga gaccaggatg aatccctgct tgccgcgcag atactggctg accatctgac
     1801 ggccgttcat atccagatga ttggtcggtt catcgatcag caggaaacgg tttcccttta
     1861 gaaacaaggc cgccagcagc accttggttc gttccccgtt ggacagcgta gcaaacggac
     1921 ggtccaggac atcgtgggcg acttccagca gattcagttc cctgagcagc tgccaatcct
     1981 catattcatc gttcatcgag cgaatgacgg tagcggtatc catcccctca tcctgcactt
     2041 cgtacggaaa atattcaaac gacacgctgg ccgagatgct gccgcggtac tcgaacttcc
     2101 ctcgcagtaa attcagaaaa gtggttttcc cgcggccgtt gcggccggta aaacccagct
     2161 tccaatcggt atcgatctgg aaactgacat tttcaaagat attttcgatt cccccatcat
     2221 aaccaaaggt gagatggttg acattgatca gtgacatggc gctgttcctc ctgcagaaaa
     2281 taattatgag atgcaggaaa gcagtcccca gcatctcatg gcagcgcaac atatacagcg
     2341 tgttcacgac aaaatacagt aacccatgag ttttccggtg cagcaacttt cccgcccgag
     2401 cacaaccaaa ccggcatcgt caatgccaat cttgttgccc cctgtatctt agttcatcag
     2461 caggaaaact agcgcatcct tccaactcct atctttacat ctataaatcg attataacag
     2521 gaacgacctg ccgaaggaat acccatcgac aatttactta accgcctgac gtgttgaaca
     2581 ttattttttt ccattaaaat attcttctga aacctgaagc tgttttttga ggatctctat
     2641 ccatagatgt cctttgattt ctccgctacc catggagatc ttggtacgtt tcaacgtgcc
     2701 gtcgggcatg gattttcggt aataccaatg atcagtcact ttataaagct cccacccatc
     2761 tctttcgcaa aacctcttca ggtctctcca gctaggcatg gatcaaggcc ttcacttcat
     2821 taagatcttc cgccagcagc actctcaaga cataaggaaa atgctgtttg cggttaatgg
     2881 cattaaaata taattgaaac tgatcgtaat actccattgc atactcaaca agttcctcag
     2941 ccagtctcgt tatcgcctct tcccgatccg aagcatttat aaccagatca aaaccgtcca
     3001 aggtcgcggt gatcgagccg tcctcttctg attgaaaaac agctttgatg ctgtaatcac
     3061 tcaataaact caaaacctga gcattggaaa gcaacatcag attatcccgg tttcttttaa
     3121 atgccacggg tttttctcta acaactgtat cgatagcaga tgaaaaattc tttcgtaatt
     3181 cagttgccgg tatagtttgc ataaatccca ccatcctcac ccccattgta cattatgtac
     3241 atagtgtacg caatggcagg agataatatg aaaataactt ggggtcaggc cccaagttat
     3301 tttttccagt ttttcaccat cgctcgccgc tccagcagtt cggccaagta ggtccggccg
     3361 ctgctctggc aatgatttaa cagcgccagc gtggcccgca cgtcgtcttc ggcgcgatgc
     3421 tgacggtcgg gacgaattcc gtgccaggtc agcaagtcct gtaatcggcg gttggggtac
     3481 cccttgccgc gccaattgat gcctgacatc gagcaatacc agtgtttgta ggctgcctgg
     3541 ggatacaagc gcgtcacaaa accccggtca aacgaagcat tatgggcaat gaaaaattcc
     3601 gcccggcgga tcagctcatt gacccgctga tggtccaggt cgcgcccgca gacatccgcc
     3661 cagccaatcc cgttgacccg ggccgccgcc gcattgatgc cgttgaccgg ctcccgcagg
     3721 ccagtgtatg cgtccacaat ctccgccaca ccgtgatgat tcacacaaaa taaaatgatg
     3781 caaagctcga cgatctcatc ccgatccggg ctaaaaccgg tcgtctccac atcaacgatg
     3841 gcaccgagcg ggtttatcgg tgaggaatgg ttcatgttta acctccatgg gggttgttaa
     3901 tattacttct ataatactat ttttagattt caatattaaa attagatttc cttcatatta
     3961 cctaagcctc tgaacggagc actgctccaa ttgttcaacg gttattataa cattcccatt
     4021 tctatctaca cgctcgtgcg gggcgtgact aacgcgttgg caagtttcac actctttact
     4081 ggtgtttcaa aatatattta ttc
//
LOCUS       DAOTHZ010000131         4037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_26356_length_4037_cov_8.006278, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000131 DAOTHZ010000000
VERSION     DAOTHZ010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26356_length_4037_cov_8.006278"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="P5549_10155"
     CDS             <1..441
                     /locus_tag="P5549_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW13475.1"
                     /translation="SAVVLMLILQYIIKWTKTGKAMRAVSLDQEAAVLMGISVDKTIS
                     ITFAIGSALAAAAGVMYGVYYSTVNPLMGIIPGLKAFVAAVLGGIGIIPGAMAGGFIM
                     GLLETMVSGYGNSLYRDAVAFGVLILILLIKPTGLFGKDSGEKV"
     gene            451..1401
                     /locus_tag="P5549_10160"
     CDS             451..1401
                     /locus_tag="P5549_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRW13476.1"
                     /translation="MQEYKNNWAKFCGEVLLLAAFFTLVQYLITTGLLNQYYQINLNS
                     MFINIILAVSLNLINGFTGQLSLGHAGFMAVGAYSSVILTQMMGQPFIAGLIGACLVS
                     ALAGFIIGVPTLRLKGDYLAIATLAFGEIIRVILQNIDYVNGPAGILGITKMTTWPWL
                     FGATVLTILVIVNLINSSYGRAIISVREDEIASELMGVNTTSYKVLAFVIGAMFAGLA
                     GALYAHYFYIIKPETFNFLKSFDILVMVVLGGLGSTTGAVIAAVFITALTAALQSFPA
                     IRMILYALILIIVMIFRPQGLMGNKELSRQTFGKIWSGRK"
     gene            1398..2165
                     /locus_tag="P5549_10165"
     CDS             1398..2165
                     /locus_tag="P5549_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13477.1"
                     /translation="MSLLEINNLNKAFGGLSAVLNFNFHMEKGELVGLIGPNGAGKTT
                     VFNLITGVYKPDKGSIRFSDKNIQGLAPYTICARGIARTFQNIRLFNDLSVLDNVKIA
                     AHKDVPYGLLTAIMRTPSFFKGEEIIEQQAMKYLEIFKLADKRDELARNLPYGEQRRL
                     EIARALATKPTLLILDEPAAGMNPYETNELMELIAWIRKDFDLTILLIEHDMSLVMGV
                     CERIYVLDYGSIIAEGVPQEIRNNKKVIEAYLGEEVE"
     gene            2165..2869
                     /locus_tag="P5549_10170"
     CDS             2165..2869
                     /locus_tag="P5549_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13478.1"
                     /translation="MLKVNDIDVYYGAIHALKKLSLEVEQGTIVTLIGANGAGKTTTL
                     KTISGILRPKSGTITYKGSEITKVAPEKIVSLGISQVPEGRRIFPTMSVLENLEMGAY
                     LRKDKKEIGKDMENVFVRFPRLLERRKQMAGTLSGGEQQMLAIGRALMAKPELLLMDE
                     PSMGLAPLLVKEIFEIIKDINSRGTTILLVEQNANMALSVADKAYVIETGEIVLQGSA
                     EQLMKSEDVKKAYLGG"
     gene            2911..3570
                     /locus_tag="P5549_10175"
     CDS             2911..3570
                     /locus_tag="P5549_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HRW13479.1"
                     /translation="MKVKNRMTENPRTVSLDSSVTEAFSLMKENNIRRLPVVDKSKVI
                     GIITISDLNQATPSMATTLSIHELNYLLAKTKIKDVLPKKQKLITIGPENYIETAAKL
                     MRINKVSGLPVIDDQENLVGIITETDLFDAFIDILGVYRPHTRIDFYTSDRPGTIAGI
                     TGLIAEKGKNILNTVVFYDKKKSKYKMVIRIEELDCQDVVEALKDRGHEVESVIITHP
                     E"
     gene            3751..3849
                     /locus_tag="P5549_10180"
     tRNA            3751..3849
                     /locus_tag="P5549_10180"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3785..3787,aa:Sec,seq:tca)
BASE COUNT         1099 a          978 c          980 g          980 t
ORIGIN      
        1 tcagccgttg tgctgatgct gattcttcaa tatatcatca aatggaccaa aaccggaaaa
       61 gccatgcggg cagtttccct ggatcaggaa gccgctgttc tcatgggcat tagcgtggac
      121 aaaaccattt ccattacctt tgccattggc tctgctttgg cagccgccgc aggagtaatg
      181 tacggtgttt attatagtac agtaaatccc ttgatgggca tcattcccgg actgaaagct
      241 ttcgtggcgg cagttctggg aggcataggc atcattcccg gagccatggc gggaggtttc
      301 attatgggat tattggaaac catggtcagc ggttacggca acagcctcta ccgtgatgca
      361 gtcgcctttg gcgtgttgat tctgatcctt ttgatcaagc cgaccggtct ctttggtaaa
      421 gattccgggg aaaaagtgta ggtgattgag atgcaggaat acaaaaacaa ctgggccaaa
      481 ttctgtgggg aagtactttt gttggccgcc ttcttcacct tggtacaata tctgatcacc
      541 accgggctgc tgaatcagta ttatcagatt aacctgaatt caatgtttat caacatcatt
      601 ctggcagtca gccttaacct gatcaacggg tttaccggtc agctgtcgct gggacatgcc
      661 ggctttatgg ctgttggagc ctattccagt gtcattctta cacaaatgat gggacagccc
      721 ttcattgcag gcctgatcgg ggcctgtctg gtctccgcgc tggccggctt tatcatcggg
      781 gtgcccaccc tgcgcctgaa gggcgactat ttggcgattg ccaccctggc ttttggagaa
      841 atcatccgcg ttatcctgca gaacattgat tatgtaaacg gaccagccgg tattctggga
      901 attaccaaga tgaccacctg gccctggctg tttggtgcca ctgtcctgac catcctggtg
      961 attgtcaacc tgatcaattc cagttatggt cgggccatca tttccgttcg ggaagatgag
     1021 atcgcttcag agttgatggg cgtcaacacc accagctata aagtcctggc ctttgtcatc
     1081 ggcgccatgt ttgccggtct ggccggtgct ttgtacgccc attattttta tattatcaaa
     1141 cccgagacct ttaacttcct gaaatcattt gatattctgg tcatggttgt gctgggcgga
     1201 ctgggcagca ccaccggcgc agtgattgcc gccgttttca ttaccgcctt gaccgctgcg
     1261 ctgcagtcat tcccagccat cagaatgatt ctctacgccc ttattttgat cattgtgatg
     1321 atcttccggc cgcaaggact gatgggcaat aaagaactga gccgacagac atttggcaag
     1381 atttggagtg gacgaaaatg agtcttttgg aaataaataa tctcaacaaa gcgtttggcg
     1441 gcctgagcgc agtgctgaac ttcaactttc acatggaaaa aggcgaactg gtcggattga
     1501 tcgggcccaa cggggccggc aagaccaccg tctttaatct catcaccggc gtatataaac
     1561 ccgacaaagg cagtatccgc ttcagtgata aaaacatcca ggggcttgcg ccctatacca
     1621 tatgcgctcg cggcattgcc cggacctttc aaaacatccg gttgttcaat gacttgtcgg
     1681 tgttggataa tgtcaagata gccgcccaca aggatgtccc ttatggactg ttgaccgcca
     1741 tcatgagaac ccccagtttc tttaaaggcg aagaaataat cgaacaacag gccatgaaat
     1801 atctggaaat cttcaaactg gcggataaac gcgatgaact ggccaggaac ctcccctacg
     1861 gagaacagcg ccgcctggaa attgcccggg cgctggccac caaaccgacc ttgttgatcc
     1921 tggacgagcc ggctgccggc atgaatccct atgagaccaa tgaactgatg gaattgattg
     1981 catggattag aaaggatttt gatttgacga ttctcctcat cgaacacgat atgtccttgg
     2041 tgatgggcgt ctgtgagcgg atctatgtgc ttgattacgg cagcatcatt gccgaaggag
     2101 taccccaaga gattcgcaac aataaaaaag ttatcgaggc ctatttggga gaggaggtag
     2161 aatagtgcta aaagtcaacg atattgatgt atattacggt gcgattcacg ccctgaaaaa
     2221 attatccctg gaagtagagc agggaaccat tgttaccctg atcggcgcca atggtgccgg
     2281 caaaacgacc acattgaaaa ccatttccgg cattttacgg cccaaaagcg gcacgataac
     2341 ctacaaagga agcgaaatta ccaaagttgc cccggaaaag atcgtcagtc tgggaatatc
     2401 gcaggtcccg gaaggccgcc ggattttccc caccatgtcc gtgctggaaa acctggaaat
     2461 gggggcctat ctacgcaaag acaaaaaaga gattggcaaa gacatggaaa atgtttttgt
     2521 ccgcttcccc cgtttattgg agagacgcaa acagatggcc ggaaccctct ccggcggcga
     2581 gcagcagatg ctggccattg gccgcgcact gatggccaaa ccagaattgt tgctgatgga
     2641 tgaaccctcc atgggattgg cgccgctgtt ggtcaaagaa atctttgaaa tcatcaaaga
     2701 cataaactct cgaggaacga ccatcctgct ggtggaacag aacgccaaca tggcattatc
     2761 cgtagccgat aaggcatatg ttatagaaac cggtgaaatc gtactgcaag gcagtgccga
     2821 gcaactgatg aagagcgaag acgtcaagaa agcctatctg ggaggctagc aagcagaaac
     2881 cacaagtaat attagcaggg aggcaagagc atgaaagtaa agaatcgtat gactgaaaat
     2941 ccgcgtacgg tatcattgga ttccagtgtg accgaagcat tttccctcat gaaagaaaac
     3001 aacatccgcc gtctgccggt ggtagacaaa agcaaagtca tcggcatcat caccatctcc
     3061 gatttaaatc aggccactcc ttcaatggcc actaccctga gcattcatga actcaattac
     3121 ctgctggcta aaaccaagat caaggatgtc ctgcccaaaa aacaaaagct tatcaccatt
     3181 gggccggaaa actatatcga gactgctgcc aagctgatgc gcataaacaa agtcagcggt
     3241 ctgccggtca tcgatgacca ggaaaacctg gtcggcatca ttaccgaaac cgatttattt
     3301 gatgccttta tcgatatcct gggtgtctac cgaccccata cccggatcga tttttacacc
     3361 tctgaccgtc ccggaacgat tgccggcatc accggcctga ttgccgaaaa agggaaaaat
     3421 atcttaaata ccgttgtttt ctatgacaaa aagaaaagca agtacaaaat ggttatcaga
     3481 atcgaagaac tggactgtca ggatgtcgta gaagccttaa aagaccgcgg ccacgaagtg
     3541 gaatccgtca tcatcaccca tcccgaataa agaaaagctt gaagaggagg gggcaggctt
     3601 tgactcgcag acatgtgctt ttaaaaacat gatgccgcgc caagccagcc tctttttatc
     3661 tggccaatcc ggcaaaacaa ccggtttgac atttttaaca aaggataata tactcaggta
     3721 gcgagcagcg catgtcgaaa atcaaatcat ggaggcggat cggtcctggt gggtcgcctg
     3781 gacttcaaat ccagtttgcg ccgcagtgat cctgcggcgg gtgagttcga ttctcacacg
     3841 cctccgccaa ataaaatatt ttaaaacctt gtaaatatcg taaaaatgcg atttttcagg
     3901 gttttttatt tcaatttaag cgtgttggaa atggttaata atatacgttt ttatctacca
     3961 atctacacag taactacaca ttgtttcaat cccgcacaac tattaaaatt accgagccgc
     4021 ctgattaagc aggacag
//
LOCUS       DAOTHZ010000132         3954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_27096_length_3954_cov_7.045396, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000132 DAOTHZ010000000
VERSION     DAOTHZ010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27096_length_3954_cov_7.045396"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..3485
                     /gene="rpoC"
                     /locus_tag="P5549_10185"
     CDS             3..3485
                     /gene="rpoC"
                     /locus_tag="P5549_10185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRW13480.1"
                     /translation="MASPEQIRSWSSGEVKKPETINYRTLRPEKEGLFCEKIFGPVKD
                     WECHCGKYKRVRHRGIVCDRCGVEVTTSKVRRERMGHIELAAPVCHIWYLKGIPSRMG
                     LLLDMSPKVLEKVIYFVNYIVIDPGDTPLLKKQLLNEREYRENRERYGDSFYAAIGAE
                     AIKELLAEVDLEALSLELKEEIATSTGQRKGRGIKRLEVTESFRLSNNNPEWMILDVL
                     PVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPDIIVRNEKRMLQ
                     EAVDALVDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVG
                     PKLAMHQCGLPKEMALELFKPFVMKKLVDRGIATNIKSAKKMVERGREEVWDILDDVI
                     KEHPVMLNRAPTLHRLGIQAFEPVLVEGRALQLHPLVCTAYNADFDGDQMAVHVPLSA
                     EAQTECRLLMLASNNILNPKDGKPVVTPTQDIVIGVYYLTYVGQADWDARTNPKAFSS
                     PNEAQMAYELGNIQLHEKIKVRMKMTPAMISQAEENGQAIKSPYYLNETTVGRVIFNE
                     IIPDSLGYYNETIDKKKLGNIVYECYRLEGNALTAQMLDGIKRLGFRYSTRAGLSVGV
                     TDFQTPVEKEAILDKADIEVEDIEQDFLMGLITEDERHAQVVEIWNEATEEVTEALKQ
                     SLEEDNPVYMMATSGARGNFQQIRQLAGMRGLMADPQGKIIDLPIKANFREGLTVLEY
                     FISTHGARKGLADTALRTADSGYLTRRLVDVSQDVIVREEDCESVEGIWVEKIMEGSQ
                     LIEPLNQRIIGRVSVDTIIHPETGEILVEENQMIDDEQGFAIERCGIDRVKIRSVLTC
                     KTPHGVCKKCYGRNLATGYDVEIGEAVGILAAQSIGEPGTQLTMRTFHTGGVAGEDIT
                     RGLPRVEELFEVRKPKGQALVAESSGRIRMGESKGRREVQILGDNEEILASALVHYGS
                     RLKVQEGDWVDIGDALTEGPLNPHDILKTKGVQAVQRYLLQEVQKVYRSQGVDISDKH
                     IEIMIRQMMKRVKIEDSGDTTHLPGAFVDVAYFEEENYRAITDGGLPSVCSPMLLGIT
                     KASLNTDSFLSAASFQETTKVLTEAAIKGKVDSLIGLKENVIIGKLIPAGTGYSIYKN
                     VDVVDNQEPELLAE"
     gene            3670..3918
                     /locus_tag="P5549_10190"
     CDS             3670..3918
                     /locus_tag="P5549_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRW13481.1"
                     /translation="MSLNDLQEGKKVIGTKQVKKAIIKGQTKKVYLADDAEPHIINAL
                     RVLCQQHQIEIEETNKMEELGNACGIEVGAAAVALLID"
BASE COUNT         1183 a          792 c         1033 g          946 t
ORIGIN      
        1 ccctggcttc tcctgaacaa attcgttcct ggagcagcgg tgaggtaaaa aaacccgaaa
       61 ccatcaatta tcgtaccctg agaccggaaa aagagggttt gttctgtgaa aaaatatttg
      121 gaccggtaaa agactgggaa tgtcattgcg gaaaatacaa aagggttcgt cataggggta
      181 ttgtctgtga ccggtgcggg gtggaagtaa ccacctccaa agtcagaaga gaacggatgg
      241 ggcacattga gctggctgct cctgtatgtc acatctggta tttgaagggt attcccagcc
      301 gtatgggttt gttgctggat atgtcgccca aggttttgga aaaagtaatc tattttgtta
      361 actatatagt aattgatccc ggggatacac cgctgctgaa gaaacaactt ctcaacgaac
      421 gtgaataccg ggaaaacaga gaaagatatg gagatagctt ctatgccgcg attggtgccg
      481 aagccatcaa agaactgttg gcggaggttg atctggaagc tttatccttg gaattaaaag
      541 aagaaatcgc aaccagtacc gggcagagaa agggacgcgg gatcaagcgg ctggaagtaa
      601 ccgaatcctt ccgtttatca aataataatc cggaatggat gattttggat gttttgccgg
      661 ttattcctcc cgaattaaga ccgatggtac agttggacgg gggacgcttt gctacttccg
      721 atttaaacga tttgtaccgc cgggtcatca accgcaataa tcgtctcaaa cgattactgg
      781 atctgggtgc accagacatc attgtgcgta acgaaaaacg tatgctgcag gaagcggtag
      841 acgcgctggt agataatggt cgtcgcggca gaccagtgac tggtccgggc aaccgtccct
      901 tgaaatccct gagcgacatg ctcaagggaa aacaaggccg tttccgtcag aacctgctgg
      961 gtaaaagggt tgattactca ggaagatcgg ttattgtggt aggcccaaaa ctagccatgc
     1021 accagtgtgg attgcccaaa gaaatggccc tcgaattatt taaaccattt gttatgaaaa
     1081 aactggttga tcgcggtatc gctaccaata ttaaaagtgc taaaaaaatg gtggaaagag
     1141 gcagggaaga agtttgggac atattggatg atgttatcaa ggagcaccca gtcatgttga
     1201 accgtgctcc tactctccac agacttggta ttcaagcctt tgagccggtg ttggtggaag
     1261 gacgcgctct gcaattgcat ccgttggtat gtacagcata caacgctgac tttgatggtg
     1321 accagatggc cgtacatgtg ccgctgtccg ctgaagcaca aaccgaatgc cgtttgctga
     1381 tgctggccag caataatatt ttaaatccca aagacggaaa acccgtagta acacctaccc
     1441 aggatattgt tatcggggtg tattatctta cctatgtcgg tcaggctgat tgggatgcgc
     1501 gaacaaatcc taaagcattc agcagtccca atgaagctca gatggcatat gaattgggaa
     1561 atatccaatt acatgaaaaa atcaaggtca ggatgaaaat gaccccggcc atgatcagtc
     1621 aagcagagga aaacggacaa gcgataaaat caccttatta tttaaatgaa acgacagtgg
     1681 gcagggttat ttttaatgaa ataattcccg acagcctggg ttattacaat gaaaccattg
     1741 ataaaaagaa attgggcaac atcgtatatg aatgctatcg cttggaaggc aatgccctga
     1801 ccgcccaaat gcttgatgga ataaagcggc tgggattccg ttattcgacg cgggcaggcc
     1861 tgagtgttgg cgttaccgac ttccaaacgc ctgtagaaaa agaagccata ctggataagg
     1921 ctgatataga agttgaagat attgaacagg atttcttgat gggattgatt acagaagacg
     1981 aaagacatgc gcaagtggtt gaaatttgga atgaagcaac cgaagaagtc accgaggctt
     2041 tgaaacaatc cctggaagaa gacaacccgg tttacatgat ggctacctcc ggtgcgcgcg
     2101 gtaacttcca gcagatcaga cagttggcgg gcatgcgtgg attgatggca gatccccagg
     2161 ggaaaattat agatttgccg atcaaagcca acttccgtga aggcctgacg gttctggaat
     2221 attttatttc cacccacggc gcccgcaaag gtttggcaga cacagcgctg cgcaccgctg
     2281 actcagggta tctaactcgt cgtttggtgg atgtatccca agatgttatc gtcagggaag
     2341 aagactgtga atcggttgaa gggatctggg tagaaaaaat catggaaggt tcccaactga
     2401 ttgagccgtt aaatcaaagg atcattggcc gggtgtcggt ggataccatc attcatccgg
     2461 aaacaggtga aattctggtt gaggaaaatc agatgatcga tgacgagcag ggttttgcaa
     2521 tcgaacgctg cggtattgat cgggtaaaga tacgctctgt gctgacctgc aaaacaccac
     2581 acggcgtatg caaaaaatgt tatggacgaa atctggccac aggttatgat gtagaaatcg
     2641 gagaagcagt tggaatactg gcagcacaat cgattggtga acccggtacc cagttgacca
     2701 tgcgtacctt ccataccgga ggagtcgccg gcgaagatat tacccgcggt cttccccgag
     2761 ttgaggaact gtttgaagtc cgcaaaccca aagggcaagc cttggttgca gaatcttcag
     2821 gaagaatcag aatgggtgag agcaaaggac gccgggaagt ccagatattg ggcgataatg
     2881 aagagatttt ggcttctgcc ttggtccact atggttcccg cctaaaggtg caggaagggg
     2941 actgggttga tatcggtgat gcacttacag aaggaccact caacccacat gatattttga
     3001 aaaccaaagg cgttcaagcg gtacagcgct atttgttgca ggaagtacaa aaggtttatc
     3061 gttcccaagg ggttgatatc agtgacaaac acattgagat catgatccgg cagatgatga
     3121 agcgagttaa gattgaagat tcgggagata caactcattt gcccggcgcc tttgttgatg
     3181 tagcttactt cgaagaggaa aactatcggg caataaccga tggggggttg ccttccgttt
     3241 gttctcctat gttgctgggc attaccaaag catccttgaa tacagattcg ttcctgtccg
     3301 cggcttcttt ccaggaaacc accaaagttt taacagaagc ggctatcaag ggaaaggtgg
     3361 acagcttgat cggcttgaaa gaaaatgtta ttattggtaa gctgattccc gcaggaacag
     3421 gttattcaat ctataaaaat gtagatgtag ttgataacca ggaaccggaa ttactggctg
     3481 aatgattggg aaaagataaa taaagatcga agcaaggctt tcctgttgca atcaatcaca
     3541 ggaaagcctg attctcctta aatttagttt gactgcgcac aaccttggtg ataaaatatt
     3601 aaggtgcgcg acaaataagc tcatatattc gctaaaaatg aggtttactt gtcgggagga
     3661 ggataagggt tgtcactcaa cgatttgcag gaaggcaaaa aggtaatcgg caccaaacag
     3721 gttaaaaaag ccattatcaa aggccaaacc aaaaaagttt atcttgctga cgatgctgaa
     3781 cctcatataa taaatgctct tcgtgtactc tgtcagcaac atcagataga gattgaagag
     3841 accaataaga tggaagaatt gggtaatgcc tgcggcattg aagtaggcgc agcagcagta
     3901 gcattactga ttgattaaac ggggaaggag gtgcaggtat gccgaccatc aatc
//
LOCUS       DAOTHZ010000133         3953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_27101_length_3953_cov_8.076963, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000133 DAOTHZ010000000
VERSION     DAOTHZ010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27101_length_3953_cov_8.076963"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..564
                     /locus_tag="P5549_10195"
     CDS             13..564
                     /locus_tag="P5549_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRW13482.1"
                     /translation="MNRQEALTLLQQHLKSENLINHSLAVEAIMRGLAEYLHEDVEKF
                     GLAGLLHDIDYDVTADDPHRHSLLGAEILREIGLDSEIVQAVKVHNDMHGLERVTLLD
                     KTLWAADPVSGFITAAALVRPDKSLAEVQLKSLKKRFKETAFARGANREQMKSCQDLG
                     LELDQYLEIALHSMQKIAGEIGL"
     gene            649..2754
                     /gene="rnr"
                     /locus_tag="P5549_10200"
     CDS             649..2754
                     /gene="rnr"
                     /locus_tag="P5549_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRW13483.1"
                     /translation="MLNKETIIAYMRQESYRPLSYHDLHAALQLTEEDHARFSEMIGQ
                     LEKEGNIVRTRKDKYGLPEMMNIYRGIIKLSQRGYGILIPDQIGVPEIFVYGRNLNGA
                     MHEDRVLVRIHERASEDQRPEGEVIRAIARASQELVGTFERGKHAMQVIPDDPRQIYP
                     INVKPSSKLKLKNGDKVLVKINRWPDKKKYPEGIITEVFGRQGQPGVELQIVIKKHGL
                     REHFPAGVVEEARNVAQPVSPEEIEKRRDLRSLRMVTIDGDDAKDLDDAVSIEKIAEG
                     YRLGVHIADVSHYVREDSKLDKEAFARGTSVYLIDKVLPMLPPELSNGICSLTAGQDR
                     LAITCMMDIDPKGKVLRHDLFKSVIHINERMTYTAVNHIITHDDPAIKERYHELVDDF
                     LLMKELADIIRQERSRRGALDFDFPESKVIVDENSFPVEIRRFERGAGEMLIEDFMIK
                     ANETVAEYIYWQEIPLLYRVHEKPEGESLAKLNKVLGVFGYKIAEEKAEPFVFQKILK
                     DIKDSPVENVISLLVLRSMKHARYLPQELGHFGLAAKYYCHFTSPIRRYPDLIVHRVL
                     TLLLEGRMNSRKKAAMEARMGKYAEQSSLQEAKAEEAERELVDIKKAQYMQQFIGDEF
                     DARIASVLSFGFFVELPNTVEGLVHISTIADDYYIFNDHAYTLTGTHGGKKYAIGDQV
                     RVQLVSVDVDAAKIDFELV"
     gene            2941..3414
                     /gene="smpB"
                     /locus_tag="P5549_10205"
     CDS             2941..3414
                     /gene="smpB"
                     /locus_tag="P5549_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRW13484.1"
                     /translation="MGKKEGIKPVVDNRKARHDYHIKDVYEAGMVLVGTEVKSLRAGK
                     GNLKDAYAVVKGGEVWVNNFHISPYEKGNQFNHEPLRPKKLLLHKAEINKLLGIQKES
                     GYTLIPLKIFFKNGLAKMDLAVAVGKKNYDKREDIANRDAKRDIERVMKSRHQEN"
     gene            3603..3953
                     /gene="ssrA"
                     /locus_tag="P5549_10210"
     tmRNA           3603..3953
                     /gene="ssrA"
                     /locus_tag="P5549_10210"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1099 a          973 c         1066 g          815 t
ORIGIN      
        1 ggaggatgta ccatgaaccg acaagaagca ctgacattgt tgcagcagca tcttaaaagc
       61 gaaaatttga tcaaccacag tctggcagtc gaagccatca tgcgtggcct ggccgaatac
      121 ctgcacgaag atgtagaaaa gtttggcctg gcaggcttgc tgcacgacat cgactatgat
      181 gttacggccg acgaccccca ccgccattcc ctgctgggcg ccgagatctt gcgcgaaatc
      241 ggcctcgatt cggaaatcgt gcaggctgtc aaagtacata atgacatgca cggactggaa
      301 agagtcaccc tgctggacaa aaccctctgg gcagccgatc cggtaagcgg cttcattacc
      361 gcggcggcgc tggtcagacc cgacaaaagt ctggctgagg tgcagctcaa atccttgaaa
      421 aaacgcttca aggagaccgc ctttgcaagg ggtgccaacc gcgagcaaat gaaaagctgt
      481 caggacttgg ggctggaact ggatcaatat ctggagatcg cactgcacag catgcaaaag
      541 attgccggtg agattggtct ttaagtttaa aagttgcagc gatgggaaaa aattagataa
      601 cgaggggatc atcccatcgt taacaagaaa gaataaaagg tggcatacat gctgaacaaa
      661 gaaaccatta ttgcctatat gcgtcaggag agttaccggc ccttgtcata tcatgacctg
      721 cacgccgcat tgcagttgac tgaagaagat catgcccgtt tcagtgaaat gatcggccaa
      781 ttggaaaaag agggaaacat cgtacgtacc cgcaaggata aatatggcct tcccgagatg
      841 atgaacattt atcgcggcat catcaagctt agtcaacgtg gctacgggat actaatcccc
      901 gatcagatcg gggtgccgga aatattcgtc tatggacgca atttaaacgg ggccatgcat
      961 gaagaccgcg tgctggtccg catccatgag cgcgccagcg aggatcagcg cccggagggg
     1021 gaagtgatcc gcgccattgc cagagccagc caagaactgg tgggcacctt tgagcgggga
     1081 aaacatgcca tgcaggttat tcccgatgac ccccgccaga tctaccccat caatgtcaaa
     1141 cccagcagca aactaaagct taagaacggc gacaaggttt tagtcaagat caaccgctgg
     1201 ccagacaaaa aaaaatatcc cgaaggaata atcaccgaag tatttggccg gcagggacag
     1261 cccggggtgg aattgcagat cgttatcaag aaacacggac tgcgggaaca ttttccggcc
     1321 ggggtagtgg aggaagcccg taacgtagca cagccggtca gtccggaaga aattgaaaag
     1381 cggcgtgatc tgcgcagttt gcgaatggtc accattgacg gcgatgatgc caaagatctt
     1441 gatgatgccg tttccattga aaaaatcgcc gaaggctatc gcctgggcgt gcatatcgcc
     1501 gacgtatccc attacgtccg cgaggacagc aagctggaca aggaagcctt tgcccgtgga
     1561 acctcggtat atctgatcga caaagtactg cccatgctgc ccccggaatt atccaacggc
     1621 atctgcagcc tgaccgccgg acaggatcgg ctggcaatta cctgtatgat ggacattgac
     1681 cccaagggca aggtactcag acacgatctg tttaaatccg tcatccacat caacgagcgc
     1741 atgacctata ctgccgtcaa ccacattatc actcatgacg accccgcgat caaggaacgt
     1801 tatcatgaat tggtggatga tttcttattg atgaaagaac tggcggatat tatccggcag
     1861 gagcgcagcc gccgcggcgc gctggacttt gattttcctg aaagcaaggt cattgttgac
     1921 gagaacagtt tcccggttga gatccggcgc tttgagcgcg gcgcaggcga aatgctgatc
     1981 gaagacttta tgatcaaggc caatgaaacc gtagctgagt atatctattg gcaggaaata
     2041 ccgctgcttt accgggtgca tgaaaaaccg gaaggcgaat cgctggccaa actcaacaaa
     2101 gtgctgggcg tatttggcta taaaatcgcc gaagaaaaag cagagccgtt cgtttttcaa
     2161 aagattttaa aggacatcaa agactcacca gtggaaaatg tgatttccct gctggtgctg
     2221 cgatccatga aacacgcccg ctatttgcct caggaactgg gacattttgg gctggccgcc
     2281 aaatattatt gccattttac cagccccatc cgccgctatc ccgacctgat cgtacaccgg
     2341 gtgctgaccc tgctcctgga aggcaggatg aacagcagga aaaaggcggc gatggaagcc
     2401 cgcatgggca aatatgcgga acagtccagt ctgcaggaag ccaaggccga agaggccgaa
     2461 cgcgaactgg ttgatatcaa gaaagcccag tacatgcagc aatttatcgg cgatgagttc
     2521 gacgcccgca tcgcctcggt gctgtcattc ggattttttg tggagttgcc caatactgtc
     2581 gagggactgg tgcatatctc caccattgcc gatgattact atatcttcaa cgaccatgcc
     2641 tataccctga ccggcaccca tggcggtaaa aaatatgcca tcggcgatca ggtcagagtg
     2701 cagctggtca gtgtggatgt cgatgctgcc aaaatagatt tcgaattggt ataggccggg
     2761 cagcaaggcc ggacggactt cccgcgacag ggaaagctgc ccgggccggg accttcattg
     2821 acaagcaagc ccgacctgga ttataataac gtagcatcaa gagatacacc ctggctttgg
     2881 cgtatgctgt acccgaggcg agggtttctt ttacgaggtt cgcagcaaga ggtgagacag
     2941 atgggcaaaa aagaaggcat caagccagtc gtggacaatc gcaaagcgcg gcacgattat
     3001 catatcaaag acgtatacga ggccggcatg gtgctggtcg gcaccgaagt caaatccctg
     3061 cgggccggca aaggcaatct aaaagatgcc tatgcggtgg tcaaaggcgg cgaggtttgg
     3121 gtgaacaatt tccatatcag cccttatgag aaaggcaatc agttcaacca cgaaccgctg
     3181 cggcccaaga agctgttgtt gcacaaggcc gagatcaaca aactcctggg catccaaaaa
     3241 gagtccggct atactttgat tccgcttaaa atctttttca aaaatggcct ggctaaaatg
     3301 gatctggcgg tagcggtagg caaaaagaat tatgataaac gtgaagatat cgccaaccgc
     3361 gatgccaaac gcgacatcga aagggttatg aaaagccgcc atcaagaaaa ttaaagaatg
     3421 taaaaatatc cgggtctggt gccttgcata tgcataaaaa aactaatgcg agtttaaacc
     3481 ggcgtcattg cctaaacacc ttcattgggc acaattcccg gcgggagcta tcctaattat
     3541 acctgctgac aacgccaaac cgtggtatta tgatagtaga gcagttaatc ccaataatat
     3601 aagggggcgt aaaggtttcg acgggggata ggatgggtcg agcagcgagc cgagttgtcg
     3661 ccagctcgtt aaaaaggtga aaattcaaat taactgcaga agataacttc gcactagcgg
     3721 cttaatgccg ctcgtccggc caggtttcct aacggcctgg tgcagggcgt cacttagtta
     3781 gggtaccctg aagccttctt tctcgagggg ctttaagggg aaaccaatcg gggatagccg
     3841 aaggaagctc gttcgtgggc gtccggttgg cgaaagcaaa tcacggactg cgctcggaga
     3901 gactggactg gtcttacttt cggacggggg ttcgactccc cccgcctcca cca
//
LOCUS       DAOTHZ010000134         3912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_27469_length_3912_cov_4.854809, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000134 DAOTHZ010000000
VERSION     DAOTHZ010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27469_length_3912_cov_4.854809"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..324
                     /locus_tag="P5549_10215"
     CDS             100..324
                     /locus_tag="P5549_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HRW13485.1"
                     /translation="MIYYMADPLDLLAEMKMPEQRTLSLPSGGIVNLEIIDSCRARVA
                     SIISTDPMDYLQENLQPGCLLDLSGGLPGV"
     gene            709..2943
                     /locus_tag="P5549_10220"
     CDS             709..2943
                     /locus_tag="P5549_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRW13486.1"
                     /translation="MKSRKENNSSKGWRFREEAASVVLFMLAVFLFVSLRNFHGTPED
                     QQFIGLLGTQLSKAMQIVFGQAAWLISLFLLFWSVHIGIVKKMWSIRMGGLTLLLLTI
                     MMATSILDVPEGISSWEAGLKGMGGGAVGGGLAYILLRLLGKIGSTILLLLFAILAIV
                     MIIEKPLTDIAAAIWQGIKRLGVSLSQIMFYEEESVPAAASQPASRQKIDALIIENAS
                     AALEPQVNKTEPEVISLETNSPAESNVGLKLVGSKKKEPSQDSPEIVFLGKDEPFEYL
                     KPPVEMLQDISSNRVIDKKNIKESIATLEDTFTSFGISVKVNQVSCGPAVTRYELTPA
                     PGVKISRIISLTDDLQLNLAAPGIRIEAPIPGKSAIGIEIPNAKISSVGLRNILASSA
                     FHNLNSPLAVALGEDITGNSVVAKLSDMPHLLIAGSTGSGKSVCLNGIIMSMLHNATP
                     DELKMVFIDPKMVELAIYNGIPHLMTPVVTDPKKAAVILRWMTTEMDKRYKLFAEKGV
                     RDIYRYNQNAAEKMPFIVIIIDELADLMMVSPVEVEDSICRLAQMARAAGIHLIVATQ
                     RPSVDVVTGIIKANIPSRIAFAVSSQADSRTILDMGGAEKLLGKGDMLFFPVGAIKPY
                     RLQGAFVSDGDIEKTVMFIKQQLPRQEAERINELEFKMEEMDLEADDEMFWEAVKVFV
                     ESKKASVSLLQRKLKIGYARAARLVDCMEERGIVSEIDSNKKREILIDQEQLEKLYPD
                     SKYF"
     gene            3011..>3912
                     /locus_tag="P5549_10225"
     CDS             3011..>3912
                     /locus_tag="P5549_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016284492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRW13487.1"
                     /translation="MKLTSREQKIIEVLMQDPLISQEDLAARFGISRSSVAVHISNLM
                     KKGVILGKGYVFNKKASAVVAGEVFLRVNISDGAFDCRIDTEFSGFALEVSQALVNYG
                     MVTKLLTVLGSDDAGSQIISRLKSQEVDVNNVIRARHHRTSRKVFVDGELRYAEAIDR
                     ENISTDLESRAWLLQNCDWLLIDEPYQEMLAQQPVFQEENGPVVCTCHYWQDDLPACL
                     QQYHLVVLGVANFNKLEQIQQRGLELVQAEVQNCIVTDGKSMILCFGRQNSQDFTLLP
                     NQSFDNRNDLHLFLAGLVYGLAAG"
BASE COUNT         1029 a          852 c         1041 g          990 t
ORIGIN      
        1 ccgtgatgct gtggattgcg ggttgattga tgcaatggga ggattgcagg atgccctgcg
       61 ccaattgaat accctgcggg agacaggagg tctgctgcaa tgatttatta tatggcagac
      121 cctctggatt tgctggcgga gatgaaaatg cctgaacagc gaacgttgtc gcttccgtca
      181 ggcggcatcg tgaatttgga gatcattgat agctgccggg cccgggttgc cagcattatc
      241 agcactgatc cgatggacta ccttcaggaa aacttgcagc ctggctgtct gctggattta
      301 tccggcggtt tgccaggagt gtaaacctga ttttgcaacc gggaatcagc gataaatcgc
      361 gggccggctt gacaccttta caaatagaac ggtaagatgg ggatagtgat ttacatcgtc
      421 acggggagaa attattatgc ccggttgccc caatatatcg tttcgtacag cctgtttcta
      481 aactgcattg acaagcttct tggttaagat gtttgttgaa caaggaagaa acgggttttt
      541 cgtttgagca cggaggattt tggaaccagg caggatttcc gccttggatt gacgaactat
      601 aaatggtaat atatgtaatg cacgagggaa gggtgagcag atttcatggc gcaggctaaa
      661 aaaaaggatt cccccccaaa aaccacgcgc acaccggcac gtacagcggt gaaaagcaga
      721 aaagaaaaca attcgtccaa gggttggcgt tttcgtgaag aagcagcctc agtcgtttta
      781 ttcatgctgg cggtatttct ctttgtcagc ctgcgcaatt tccatggcac tccggaggat
      841 cagcagttta tcggtttgct gggaacccaa ctatccaaag ccatgcagat tgtatttggt
      901 caggctgcct ggttgatctc cctatttctg ttgttctggt cagtacatat tggcattgta
      961 aaaaaaatgt ggtccattcg catgggggga ttaaccctgt tgctgttgac gatcatgatg
     1021 gccacgagca tccttgatgt ccctgaggga atcagcagct gggaagcagg tctgaaggga
     1081 atgggcgggg gtgcagtcgg tgggggactg gcctatattt tgctgcgcct tttggggaaa
     1141 attggctcaa ccatcctctt gctgttgttt gccatcctgg ccattgtgat gattatagaa
     1201 aaacccttaa ccgatattgc ggctgcaatt tggcaaggga ttaaacgcct tggtgtgagc
     1261 ctcagccaaa tcatgtttta tgaagaagaa tctgttcccg cggctgcttc ccagccagct
     1321 tcccggcaga aaatagatgc cttgatcata gaaaacgctt cggctgccct ggaaccccag
     1381 gtaaataaaa cagaaccaga agttatctct ctggaaacaa attcgcctgc cgagagcaat
     1441 gttggtttga aactggtcgg tagcaagaaa aaggaacctt cacaggacag tccggaaatt
     1501 gttttcctgg gcaaggatga accattcgaa taccttaaac caccggtgga gatgctgcag
     1561 gatatcagca gcaaccgggt tattgataaa aaaaacatca aggaaagcat tgccactctg
     1621 gaagacacct ttaccagttt tggtatatcg gtcaaggtca atcaagtgag ttgcggcccg
     1681 gcggttaccc gttatgaact gacaccggca ccgggcgtga aaatcagccg aatcatcagt
     1741 ctgacggatg atttgcagct taatctggcc gcgccgggca tccggattga agctcctatt
     1801 cccggaaaat cagccattgg aatcgaaata cccaatgcca agatcagcag cgtaggtctg
     1861 cgtaacatac tcgcttccag tgctttccac aatttaaaca gtccgctggc agttgcgctg
     1921 ggcgaagata ttaccggaaa ttctgtcgtt gccaaactga gtgacatgcc tcatttgctc
     1981 attgccgggt caaccggttc aggcaaaagc gtatgtctca acggaattat catgagtatg
     2041 ctgcataatg ccaccccgga tgaactcaaa atggtattta tcgatcccaa aatggttgag
     2101 ctggccattt acaatggaat tccgcatctg atgacaccgg tggtcaccga tcccaagaaa
     2161 gcagcggtca tattacgttg gatgaccact gagatggaca aacgctataa attgttcgcg
     2221 gaaaaaggag tgcgtgacat ctaccgatac aatcaaaatg cggctgaaaa aatgcctttt
     2281 atcgtgatta ttattgatga actggctgac ttgatgatgg tctccccggt ggaggtggaa
     2341 gattccatct gccgcctggc acagatggcg cgtgccgccg gcattcattt gattgttgcc
     2401 acccagcgcc cctcggttga tgtggtgacc gggatcatca aagccaatat accttcccgc
     2461 atcgcttttg ccgtttcatc ccaggcggat tcccgcacca ttcttgatat gggaggggcc
     2521 gagaagctgc tgggcaaagg cgatatgctc tttttcccgg tgggggctat caaaccgtat
     2581 cgtttgcagg gtgcctttgt ttcggatggt gacatcgaaa agacggttat gtttatcaaa
     2641 caacaattgc cgcggcagga agcggagcgc atcaacgagt tggagtttaa aatggaagaa
     2701 atggatctgg aagccgatga tgaaatgttc tgggaagccg tcaaggtttt tgtggagagt
     2761 aaaaaggctt cggtatcctt gctgcagcgt aagctgaaaa ttggctatgc acgtgctgcc
     2821 cgactggtgg attgcatgga agaaagaggc attgtctcag aaattgacag caataaaaaa
     2881 cgagagattc taatcgatca ggaacaactc gaaaaactgt atcccgattc gaaatacttt
     2941 taaatatggt agaatataaa caaaagcatt tatccataaa caatcgtttt ttgtcaggag
     3001 aatggatgat atgaaactta cttccagaga gcagaaaatt attgaggtgc tgatgcagga
     3061 tcctctgata tcccaggagg atctggcggc tcgttttggt atcagtcgtt catcggtagc
     3121 cgtacatatt tccaatttga tgaaaaaagg ggttattctt ggcaaaggct atgtcttcaa
     3181 taaaaaagcg tcggcagtgg ttgcgggaga ggtgttcctg cgcgtcaata tcagcgacgg
     3241 tgcatttgat tgccggattg acaccgagtt ctcagggttt gccctggaag tgagccaggc
     3301 cctggtgaat tatggcatgg tcaccaaact actgaccgtg ttgggcagcg atgatgcagg
     3361 cagtcaaatt atttcccgat taaagagcca ggaagtggat gtgaacaatg tgatccgcgc
     3421 cagacatcac cggacatcac gcaaagtatt tgttgacgga gagctgcgct atgccgaagc
     3481 aatagaccgg gagaatatta gcactgatct ggaaagccgc gcctggcttt tgcaaaactg
     3541 tgattggctg ttgattgatg aaccgtacca ggagatgctg gcccagcagc ccgtcttcca
     3601 agaagaaaac ggaccggttg tctgtacctg ccattattgg caggatgact tgccggcttg
     3661 tcttcaacaa taccatctgg tggtgttggg ggttgccaat tttaacaagc tggaacagat
     3721 acaacaaaga ggcctggaac tggtgcaggc cgaggtgcaa aattgcattg tcaccgatgg
     3781 gaaatccatg atcctgtgtt ttggtcgaca aaacagtcag gatttcacct tgcttcccaa
     3841 tcagagcttt gacaaccgca atgatttgca tctgtttctg gcaggtctgg tttatggact
     3901 ggcagcggga ta
//
LOCUS       DAOTHZ010000135         3880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_27754_length_3880_cov_7.291242, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000135 DAOTHZ010000000
VERSION     DAOTHZ010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27754_length_3880_cov_7.291242"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="P5549_10230"
     CDS             <1..851
                     /locus_tag="P5549_10230"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRW13488.1"
                     /translation="LKNLGKQSRMVLQDPVPRMYHYLAGSAEVMSPAQLHDPEENVIF
                     LDCADEERAGRELLSKLAQRQYTINIDHHQSNTRFGEINHIQPDMAATAELLYHLARQ
                     LSVQITPAMANALYAGIVQDTGSFHHSNTRAETFRIAADLLECGVDLVQTRIHLFESK
                     SRDEMNLLTCALHSMRYSEDGKMAWMTISYQEAEELGVLDLHPEGIINHTLMVEGVEA
                     GMLFRETQPGIIKVGFRSKGHIDVAQIASSLGGGGHRQAAGVTMEGSLEQVQERVIAA
                     VREVIK"
     gene            852..1742
                     /gene="truB"
                     /locus_tag="P5549_10235"
     CDS             852..1742
                     /gene="truB"
                     /locus_tag="P5549_10235"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRW13489.1"
                     /translation="MHGFLNINKDSGMTSFDVLRKIKQLIPKTKLGHLGTLDPMATGV
                     LPVALGHATRLIEYIEDQEKTYRAQMTLGGVSDTQDAWGKIEKTGVIRYGSEELVPIL
                     AEFTGTIEQIPPMYSAVHHQGQRLYELARQGVEVERSPREVKITSLQMLSQHSDGEGN
                     PVIELEITCSKGTYVRTLCHDIGQRLGTGAYMSRLTRTRAGMFAISQALTLAELEKIT
                     AQLNEHIIPLDVPLRHWPALTLESQEGIMRITNGNHIKAPAGSAGMMRIYHPEGHLMA
                     IARIVSEPAYTLAQPVKVFK"
     gene            1759..2685
                     /locus_tag="P5549_10240"
     CDS             1759..2685
                     /locus_tag="P5549_10240"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRW13490.1"
                     /translation="MQLIYEIENYQPGNRELYLALGNFDGVHRGHQRLIKQAVAHKNA
                     GQGISAAFIFEPHPAQLLTPDRMPRLLGTPIRKARQLESLGLELLIYNRFTRDIASLS
                     PEEFVKNFLVERMQVKEVFVGFNHTFGFKGQGTPETLQQLGQKYGFAVNVIEPVTVDG
                     ELVSSSAIRQALEEGKISQAARMLGYDPILDGIVVEGEHRGHQLGFPTANVGIEKSFN
                     IPAKGVYAARAEVNGQRYNAVVNIGSKPTFHAAYPISVEAYLFDFNSDIYGQAISLSF
                     VQKIRDEKRFDSIDALVTQIKQDAQTAQAILA"
     gene            3006..3272
                     /gene="rpsO"
                     /locus_tag="P5549_10245"
     CDS             3006..3272
                     /gene="rpsO"
                     /locus_tag="P5549_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRW13491.1"
                     /translation="MLTPEKKLEIIKSFQVHENDTGSPEVQIAILTERINYLNEHLKA
                     NKKDHHSRRGLLKMVGQRRSLLDYLKDNDFDRYRNIVTRLGLRR"
     gene            3352..>3880
                     /locus_tag="P5549_10250"
     CDS             3352..>3880
                     /locus_tag="P5549_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRW13492.1"
                     /translation="MVHKYQTEIAGRPLIVEVGQVAKQANGAALVRYGDTVVLVTATA
                     TKDPRLGIDFFPLTVDYEEKQYAVGKIPGGFIKREGRATELATLSARLIDRPIRPLFP
                     KGFRNDIHVVATVLSVEKDNAPDVTATIGASVALGVSNIPFAGPVAAVIVGMIDGQLI
                     INPTVEQVHQSDMYLS"
BASE COUNT         1031 a         1067 c          981 g          801 t
ORIGIN      
        1 ttttaaagaa tctgggcaaa caaagccgca tggttttgca ggaccccgtg ccccgtatgt
       61 accattattt ggcgggaagc gccgaggtaa tgtcgcccgc gcaactgcat gatcccgagg
      121 aaaacgtgat attcctcgat tgcgctgacg aagagcgcgc cggccgggaa ctgttgtcca
      181 agctggcgca gcgccaatat accattaata ttgatcatca tcaaagcaat acccggtttg
      241 gcgagataaa ccatattcag ccggatatgg cggcgaccgc cgaattgttg tatcatctgg
      301 cccgacagct gtcggtacag atcaccccgg caatggccaa tgccttgtat gcaggcatcg
      361 ttcaggatac cggcagtttt caccacagca acacccgggc cgagactttc cgcatcgccg
      421 ccgatttatt ggagtgtggc gttgatttgg tgcagacccg catccatctg tttgagtcca
      481 aaagccggga cgagatgaat ctgctgacct gtgcccttca cagcatgcgc tacagtgaag
      541 acggaaaaat ggcctggatg accatttcct atcaagaagc agaagaactg ggcgtgctcg
      601 atctgcatcc cgaaggcatc atcaatcata ccttgatggt cgaaggggtg gaggcaggca
      661 tgcttttccg ggaaacccaa cccggcatca tcaaggtcgg ttttcgctcc aagggccata
      721 ttgacgtagc ccaaatcgct tcatccctgg gcggcggggg gcatcgccag gccgccgggg
      781 tcaccatgga aggcagtttg gaacaagtcc aggagcgggt catcgctgcc gtcagggaag
      841 tgataaaata attgcatggt tttttaaata tcaataaaga ttcaggcatg acgtcctttg
      901 atgtacttag gaaaatcaag cagctgatcc ccaaaaccaa actcggccac cttggcacgc
      961 ttgatccgat ggccactggt gttttgccag tcgcgttggg acatgccacc cggctgatcg
     1021 aatatatcga agatcaggag aaaacctacc gcgcccaaat gacattgggc ggggtgtctg
     1081 atacccagga cgcctgggga aaaatcgaaa aaaccggtgt cattcgctat ggcagcgaag
     1141 agctggtgcc aatcctggca gagttcaccg gcacgatcga gcagattccg ccgatgtatt
     1201 cggctgttca tcaccaagga cagcgcctct acgaactggc tcgccagggc gtggaagtgg
     1261 agcgcagccc acgtgaggtg aagattacgt cgctgcaaat gctcagccag cacagcgatg
     1321 gcgaaggcaa tccggttatc gagctggaaa taacctgctc caaagggacc tatgttcgca
     1381 ccctttgtca tgatatcggc caacgcttgg ggactggcgc ctatatgtca aggctgaccc
     1441 gcacccgtgc cggcatgttt gccatcagcc aggcattgac gctggcagag ctggaaaaaa
     1501 tcacagcaca gctcaacgag cacattattc cgctcgatgt tccgctgcgt cattggccag
     1561 ccctgacgct ggagagccag gaaggaatca tgcggatcac caacggcaac catatcaaag
     1621 caccggcagg atcagccgga atgatgcgca tatatcatcc cgagggccat ctcatggcca
     1681 ttgccagaat cgtcagcgag ccagcgtata cgctggccca acccgtaaaa gtattcaaat
     1741 gatacaagga gcgtccagat gcagcttatt tatgaaatcg aaaactatca acccggcaac
     1801 cgggagctat atttggcttt gggcaacttt gacggtgttc atcgcggcca ccaacgtctg
     1861 atcaagcagg cagtagcgca taaaaatgcc ggacagggaa tcagcgccgc cttcattttt
     1921 gaaccccatc ccgcccaact gctgaccccc gaccgcatgc cgcggttatt gggcacgccc
     1981 atccgcaaag cccggcagct ggagagcctg gggctggagc tgttgatcta caaccgtttt
     2041 acccgcgata tcgcttccct cagccccgaa gaatttgtca aaaactttct ggttgagcgt
     2101 atgcaggtca aagaagtatt cgtaggcttt aatcatacat ttggctttaa aggccagggc
     2161 acgccggaaa ctttgcaaca actgggacaa aaatacggtt ttgccgtaaa tgtcattgaa
     2221 cccgtcaccg ttgacggcga actggtctcc agcagtgcca tccgccaggc gctggaagaa
     2281 ggcaaaatca gccaggccgc ccgcatgctg ggctacgatc ccatcctcga cggcatcgtc
     2341 gtggaaggag aacaccgcgg tcaccaactc ggttttccca ccgccaacgt cggtatcgaa
     2401 aaaagcttca acatccccgc caaaggcgtc tatgccgccc gcgccgaggt caacggacag
     2461 cgttacaacg ccgttgtcaa catcggcagc aagcccacct tccacgcagc ctatcccatc
     2521 tccgtggaag cctatttgtt cgatttcaac agtgacatat acggccaggc catcagcctc
     2581 agttttgtgc aaaagatcag ggatgaaaaa cgcttcgaca gcatcgacgc cctggtcacc
     2641 caaatcaaac aagacgccca aaccgcgcaa gccatcctgg cgtgattggg cagcataaga
     2701 aaaggaagcc cctaatgtga cggcgcagcc aagcaaaacg gcgcagccca accagctgaa
     2761 gacgaaggaa agtttacttt taccgtttgc tgtgctaaaa ttgtctggtg cgaataaaat
     2821 ataaaaattt ccagatataa aggtttagca cacccccgga tcgtaaataa tgaaggaaag
     2881 agcggcagcc atagttgcca aaaaaataaa agccccagag atatgaacca atggctaggt
     2941 ccggcgattt tcaccgacgc cccgcttggt cactggcgat tttattatga caaggaggtg
     3001 aaagaatgct aacacccgaa aagaaactcg aaatcattaa atcctttcag gtacatgaaa
     3061 acgataccgg atctccggaa gtacagattg ccatcttaac cgagagaatc aattatctca
     3121 acgaacatct caaagccaat aaaaaagacc atcactccag aagaggtctg ctgaaaatgg
     3181 ttggccagag acgctccctt ctggattatc ttaaagacaa tgatttcgat cgctatcgca
     3241 acattgttac cagacttgga cttcgcagat aaagagcggt atgccgctct ttcattttat
     3301 cttgctgcct aaaagcagcc ggatttatgt aaaccgaaag gagagattac catcgttcac
     3361 aaatatcaga cagaaatagc cggacggccc ctgatcgtgg aagttggaca agttgccaaa
     3421 caggccaacg gcgcagccct ggtcagatat ggcgataccg ttgtgctggt taccgccacg
     3481 gcgaccaaag acccgcgact gggcatagac ttttttccgc tgaccgtcga ctatgaagaa
     3541 aagcaatacg ccgtcggcaa aattcccgga gggttcatca aacgcgaagg acgcgccacc
     3601 gaactggcca ccctgtccgc ccggttgatc gaccgtccca ttagaccctt gtttcccaaa
     3661 ggattccgca atgacatcca cgttgtagcg actgtgctgt ccgtagaaaa agacaatgcc
     3721 cctgacgtga ccgccaccat tggcgcatcc gtcgccttgg gtgtgtccaa tattcccttt
     3781 gccggaccgg ttgccgcagt tatcgtcggc atgattgacg gacagctgat catcaacccc
     3841 accgtcgagc aggttcacca atcagatatg tatctctcgg
//
LOCUS       DAOTHZ010000136         3841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_28121_length_3841_cov_7.647649, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000136 DAOTHZ010000000
VERSION     DAOTHZ010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28121_length_3841_cov_7.647649"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="P5549_10255"
     CDS             complement(<1..188)
                     /locus_tag="P5549_10255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075459.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRW13493.1"
                     /translation="MAKVPYKTYLTEEEMPKQWYNIRADMKEQPDPMLNPGTLQPLTS
                     PEDLYPVFCEELCKQEMDG"
     misc_binding    complement(289..536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(623..757)
                     /locus_tag="P5549_10260"
                     /pseudo
     CDS             complement(623..757)
                     /locus_tag="P5549_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            893..1261
                     /locus_tag="P5549_10265"
     CDS             893..1261
                     /locus_tag="P5549_10265"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRW13494.1"
                     /translation="MNLAEYFAQTDGTVILSTANGEGKVDAAVYSKPRLFEEDSLALL
                     MSDRLSHANIQVNPYAVLLFLENGPGWNGKRIYLKKIKEERNTELARSLVRHKHGDME
                     KINVNLVYFQIEEIRPLTGD"
     gene            1462..3267
                     /locus_tag="P5549_10270"
     CDS             1462..3267
                     /locus_tag="P5549_10270"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HRW13495.1"
                     /translation="MLQSKQKGESIIMEPAKTRNPLETIGSLAQTQMMSLGSELEYQA
                     ETLKDHPAIIFEDRTITFGELNALANRYAAYFQSLGLEKGDVVALLMENRPEFLIAAS
                     GLSKLGVIVSLINNGVRKEVLAHALNICDAKMLIVGHELMEPFLEIRQQVQLKEPGLI
                     FVEKEGLDLPATEGIADLKPLLAASSDQNPTTTGTVSSDDVIVYIYTSGTTGFPKACA
                     VTQKRWLVLGNLFTIFGRMSPDMAQYMVLPLYHNSGFDIGYSMTIVSGCTMVLRRKFS
                     ARNFWDDVRKYNVKVFIYVGELCRYIYNQPEKPDDADNPLQMIAGNGMRGELMEPLRK
                     RFNFETIYEIYGATEGVGSFLNLEQIPGMCGSLDMLGRRQGEIIKYDLDKEEIVRDEN
                     GFAVKCDVGETGLLICEINQLNVFAGYVNNPQATEEKIMHNVLKLGDCYFNSGDLVCL
                     GDKDYFSFVDRMGDTFRWKSENVSTNQVSNVVNRFGQMEDVNVYGVQVPGVEGRCGMA
                     AIKLLEGAVIDWAKLGAFVCENLPSYARPYFIRLRDTIDATNSFKQMKTQLQKEGFDP
                     SVISDPLYFLDPERGVYVELTPEIYSKIVDHTIKF"
     gene            complement(3421..3747)
                     /locus_tag="P5549_10275"
     CDS             complement(3421..3747)
                     /locus_tag="P5549_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13496.1"
                     /translation="MAVLGSMGELGRYSRQGHTIVGKYAAGKSNLNRIYTYGEGAQQI
                     ARAAVKAGFPKNKTVHSVKRKTLHQHLKKELQPGCTVLVKGPNMMGMYKTAKFLKYPG
                     QFLRLY"
BASE COUNT          978 a          958 c          887 g         1018 t
ORIGIN      
        1 ccgtccattt cctgtttaca gagttcttca caaaataccg gatataaatc ttccggtgag
       61 gttaacggct ggagtgtacc gggatttaac ataggatcgg gttgttcttt catatcagca
      121 cgaatgttat accactgctt cggcatttcc tcttcggtaa gataggtttt gtacggtact
      181 tttgccattc tgataagccc ctttcatttt ttaattttat ctttttattt tcgaccagca
      241 attttgtgat gcgtttgcgc ataaaataaa aaaacccgca tccctctact aagggacacg
      301 agttttaaac tcatgcggta ccacccaaat tggccatacg acccgctcat tgccacgcac
      361 catcatgcgt gctcccttgg taacgggcgg agaatcccgt cggccactac tctgttgcca
      421 tttagcagcc accctcgtca gtccattcag cctgcctgtt cactgccgca ttctcaaccc
      481 ctgcgactct ctgtaagtgt agtatcaagc ttacttctct gactcatcag tttgcaaaaa
      541 tgtaagtact atgaatttta tcggcccgga ttctgtttgt caatagtttt ttatatgttt
      601 actccaagat atttttccca gcctacacaa tcacgcctgc ccaatgcggg agatgatcaa
      661 gatacggggg cagcttggtg taaagatccc ctttggccag attgatctga acctgggtaa
      721 gaaagaggct gcggctgagg tcggccccgc tcaaattgaa attcatctgg cttgatcgat
      781 ttgtttaagc tataatccaa atactatcac ggtcgaaact attttttaaa cagaaccgtg
      841 atggaagatc tgcttaggcc ggacagagag aattaaaaag gagtgataaa aaatgaactt
      901 agcggaatat tttgcccaaa cggatggtac cgttatcctg tcaaccgcga atggggaagg
      961 aaaagtcgat gcagctgtct acagcaaacc ccggctcttc gaagaagatt cgctggcgtt
     1021 gctgatgtcg gaccgtttga gccatgccaa tattcaggtc aatccctacg ccgtcttgct
     1081 tttcctggaa aacggcccgg gctggaacgg taaacgcatc tatctgaaaa aaatcaaaga
     1141 agaaagaaac acggagctgg ctcgctcttt ggtccgccat aagcatggcg atatggaaaa
     1201 aattaatgtc aatctggttt atttccaaat tgaagaaatc cgccctctga ccggggatta
     1261 aagcctcctc ctcttatcgc ttctccgcca gaaatgtttc acgtgaaaca tttctggttt
     1321 tttcatttct tcccaacctt gtaatattga ggttctcctg caatcggcca gaatcatttt
     1381 ttcatttttt ctctcgcacc ctatcttcta tttcaggcga ttccaatctg ttatactaat
     1441 tgcagaacgt aatatttcgt aatgttgcaa agcaaacaga aaggggagag cataatcatg
     1501 gaacctgcca agacaagaaa tcctttggaa accatcggca gcctggcgca gacccagatg
     1561 atgtcactgg gatcggaact ggagtaccag gccgaaaccc taaaggatca tccggctatc
     1621 attttcgagg accgcaccat cacctttggg gaattgaacg cgctggccaa tcggtatgcc
     1681 gcctacttcc aatcattggg tttggaaaaa ggcgatgtgg tagccctctt gatggaaaac
     1741 cggccggaat ttctgattgc cgcttcggga ctcagcaaac tgggcgtgat cgtttcgctg
     1801 attaacaacg gggtgcgcaa ggaagttctc gctcatgcgc tgaacatctg cgacgccaaa
     1861 atgctgatcg ttggccatga attgatggaa cctttcctgg agattagaca gcaagtgcag
     1921 ctaaaggagc ccggtttgat cttcgtggaa aaggaagggc tggacctccc tgccacggag
     1981 ggaatcgctg acctgaaacc gctgctggca gcctcctcag accagaatcc caccaccacc
     2041 ggtaccgtca gcagtgatga tgtgatcgtc tatatctaca cttccggcac caccggtttc
     2101 cccaaagcct gtgccgtcac ccagaaacgc tggctggtac tgggcaacct ctttaccatt
     2161 ttcggccgca tgagtccgga tatggctcag tacatggttc tgccccttta tcacaacagt
     2221 ggttttgata tcggttattc catgacgatc gtatcgggtt gcaccatggt gctgcgccgc
     2281 aaattttccg cccgcaattt ctgggacgat gtccgcaagt acaatgtaaa agtgtttatc
     2341 tatgtgggcg aactctgccg ctatatctac aatcagccgg aaaaaccgga cgatgctgac
     2401 aatcccctgc aaatgatcgc gggcaacggc atgcgcggtg agctgatgga accgctgcgc
     2461 aaacgcttca attttgaaac catttatgaa atttatgggg ctaccgaagg ggtcggctcc
     2521 ttcttgaacc tggaacaaat cccgggcatg tgcggaagcc tagatatgct gggcagacgc
     2581 cagggtgaga tcattaaata tgatttggac aaagaagaaa tcgtccgcga cgagaacggc
     2641 ttcgccgtca agtgcgacgt aggtgagacc ggtcttctga tttgcgaaat caatcagctc
     2701 aacgtgtttg cgggttatgt caacaatccc caggccacgg aagaaaaaat catgcacaac
     2761 gtcctgaaat taggcgactg ctatttcaac agcggcgacc tggtatgtct gggcgataaa
     2821 gattatttct cgtttgtcga tcgcatgggg gatactttcc gctggaaaag cgagaacgtc
     2881 tcaaccaatc aggtctccaa tgtggtcaac cggttcggcc agatggaaga tgtcaatgtg
     2941 tatggcgtgc aggttcccgg ggtagaggga agatgcggca tggccgccat caaactgctg
     3001 gagggagcgg tcattgactg ggcaaaactg ggggcctttg tctgtgaaaa tcttccttcc
     3061 tatgcccgcc cttattttat tcgcctgcgc gacaccattg atgccaccaa cagttttaaa
     3121 cagatgaaaa cccagttgca gaaggaaggt ttcgatccgt cggtaatcag cgatcccctc
     3181 tacttccttg atcctgagcg cggcgtttat gtagaactga cccctgagat ttacagtaaa
     3241 atcgtggatc ataccatcaa gttctaaccc tacaccgagg gcataaggtg gttcagagca
     3301 cactctgaac caccttcaat cattttataa gcagatgttt tggactgtgg aaacgcaaaa
     3361 tcccccacga catcatcgag atcagcaaat tcctgctggc cgtccttatc aaaatcactt
     3421 ttaatacagt cggagaaatt ggccggggta tttaaggaat ttggccgttt tatacatccc
     3481 catcatattg ggacccttca ccagaaccgt gcagcccggc tgtaattcct ttttcaggtg
     3541 ttgatgcaac gtttttcttt tgacggagtg aaccgtttta ttcttgggaa atcccgcttt
     3601 aacggcagct ctggcaattt gttgggcccc ttctccgtaa gtatatatac ggtttaaatt
     3661 gcttttacca gccgcatact ttccaacgat cgtatggcct tggcgggaat aacggcccaa
     3721 ctcccccata ctgcccaata ccgccacatt attctcccgg ctgaactccc ggctatggct
     3781 ataactggct ttttcagcac gatggacata gaatctcccc tacatcaata gatttgtata
     3841 a
//
LOCUS       DAOTHZ010000137         3800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_28511_length_3800_cov_5.087316, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000137 DAOTHZ010000000
VERSION     DAOTHZ010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28511_length_3800_cov_5.087316"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="P5549_10280"
     CDS             complement(<1..905)
                     /locus_tag="P5549_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13497.1"
                     /translation="MKRKLWLLIGLMFLFLLPASVAGAETITGEIQAPDGRQLLAADF
                     EQMLYMEYKTEDWSVYADCSIFADGDGVLRYTVDTSGPGAFKIWVVTTNECPYTNSIP
                     QTFTIVSGDLIDQPIRLTDPAVTGKILAPDGESTYIPTENDYIEIIIEKPGEFAPEQV
                     ASTAVAANGTYRLGGIAPGNYILRMDYNQTKQTYFAPAASPLVVTENNAQTINLQLTY
                     PQLKGSVMTPEGNTFIPTYPNGARVVLLDEQNDEYSMDVAGDGSYLLGGMAEGDYQIR
                     VEPSGTDLPYTASIPEEITLSNPSSA"
     gene            1240..1650
                     /locus_tag="P5549_10285"
     CDS             1240..1650
                     /locus_tag="P5549_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRW13498.1"
                     /translation="MIAFPVEQILHTMNAGYALYEFITDENGQPCDYVFIDINPAFEK
                     LTGFRAVDIVGKRITQILPAADAIHFIERYRPVAFGGQAMRFEKPSINMDKSLRIYAF
                     SPAEVILLPWWEILPKILTTNRSFPPVMNCCTVF"
     gene            1665..>3800
                     /locus_tag="P5549_10290"
     CDS             1665..>3800
                     /locus_tag="P5549_10290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRW13499.1"
                     /translation="MAFIKDHVFRYIFVNQAMAGYFDALPDEIIGKTDFDLLPLTAAK
                     NCRRTDQKALTSGTIVNAEEKVGERIYESVKFPVQLTSGHTGIGAFLKDITANRRNED
                     YLLQQLTRHSIFERSLTMDFDTLEGQCDYALRQAMHLTECQFGVIHSYAYDEMKSGSD
                     SVNRITRVSHNVDQALLTEQLKDLWQEMIKSPQALIMNDFGSSHSLQLPAGTAGHPII
                     NNVLSVPVFFEHHLKVVIILANKENGFSEFDKNEVSLLLNSFCLLFAYRHNQALTERE
                     RNKLKSIFNELPALICEFLPDSTLTFANQSYCDYFGFYNQSMVGRKFLDLIPVEDRAG
                     VLRSYASLTPENRTNVYVHKVEANGQIRLLEWRDIGFFNKMGAPVYYYSIGLDVTEKT
                     ALEEERDRLLHQFEAMINNHDAVMLLIEPNSGRILDCNPAAAEYYGYNRDELVRMKIE
                     EINMLPGEEVKMLRLQALEKGQRYFTFPHRLKNGDIRKVDVYASPILYNGQKLLFSII
                     FDVTEKENAYAEIRYISYHDYLTGLYNRRFFEEEFARLNTDEHFPLAIIMGDVNGMKL
                     INDALGNQAGDQVLTDAAELLQRFAREQDIVARVGGDEFAVILPGCNETQARSRVKQI
                     EAQAQENRQLTSPDNLLSISFGYAVQKRPGQTLDVLMKEAEGFMYANKYYDERSIKGK
                     TIAIIMNTLFEKSPREKMHSERVGKLAAAI"
BASE COUNT          959 a         1008 c          924 g          909 t
ORIGIN      
        1 gcggacgacg gattggacaa ggtgatctcc tccgggatcg aagcagtata gggaaggtct
       61 gttccgctgg gctcaacgcg aatctggtag tcgccttccg ccattccgcc caacaagtag
      121 ctgccgtctc ccgcaacgtc catcgagtat tcatcattct gttcatccag caacaccacc
      181 cgggcaccgt tcgggtaagt gggaataaat gtattcccct ccggcgtcat gacggatccc
      241 tttagctgcg ggtaggttaa ctgcagattg atggtttggg cattattttc cgtcaccacc
      301 agagggcttg ccgcaggagc aaaatacgtt tgctttgttt ggttgtaatc cattctcaag
      361 atatagttac cgggcgcaat accgcccaga cgataggttc cgttcgccgc cacagcggta
      421 gaggccacct gctccggagc aaactcaccg ggtttttcta tgataatctc tatgtaatca
      481 ttttccgtcg ggatataggt gctttcaccg tccggtgcca aaatctttcc cgtcaccgcc
      541 ggatcggtca accgaatagg ctggtctata agatcgccgc tgacaatcgt aaaggtttgc
      601 ggaatggaat tggtataagg gcactcattc gtcgtgacga cccatatctt aaaggcgccc
      661 gggcccgaag tatcaaccgt atagcggagg accccgtccc catccgcaaa tatcgagcaa
      721 tccgcatata cgctccagtc ttcagtttta tactccatgt agagcatctg ctcaaaatca
      781 gccgctaata actgtctgcc gtccggcgcc tggatctcac cggtgattgt ctcagctccg
      841 gcgacgctgg ccggcagcag gaacaagaac atcaacccga tcaacaacca cagctttctt
      901 ttcattgcca tcccctgccc tttccatttc ccacttttta tcgccaacga gtaacgcttc
      961 aaattaaggt atttcccaaa taaaccagtt tccaaggaag tattttacat cttcgacacc
     1021 caagcaaaaa ttcctttttg tcacatccgc gcaaggcaag aaccgtcccc aacttgcgca
     1081 agttggggac ggttcttgcc ttgcgcacca acgcatgttc tcccctctca ttgtcaatca
     1141 tgggtagctc ttttattatt cgacagttca tggtataaat tagaataaac aaggttataa
     1201 cttgttaggc aaaatattaa atttattagg agcgttttca tgatcgcttt tcccgtcgaa
     1261 caaattcttc acaccatgaa tgcgggttat gccttgtatg aattcattac cgatgagaac
     1321 ggccaaccct gcgattatgt atttatcgat atcaaccctg cctttgaaaa gctgaccgga
     1381 ttccgggcag ttgacattgt cggcaaaagg attacccaga ttttgcccgc agcggatgcg
     1441 atacatttca tcgagcgcta taggccggtt gcctttggtg gtcaggccat gcgctttgag
     1501 aagccctcca tcaacatgga caagtcgctt cgcatctatg cgttttcccc tgccgaggtt
     1561 attttgttac cctggtggga gatattaccg aagatactca ctacaaacag gagctttccg
     1621 ccagtaatga actgctgtac cgttttttaa actccagcag cgatatggcc ttcattaaag
     1681 accacgtgtt ccgctatatc tttgtcaatc aggccatggc tggttatttt gatgccctac
     1741 ccgacgaaat catcggcaaa acggattttg atctgctgcc gctcacggcg gcgaaaaatt
     1801 gccggcgaac cgatcaaaaa gccttaacgt caggcacgat tgtcaacgca gaagagaagg
     1861 tcggcgagcg gatctatgaa tcagtcaaat tcccggtgca gctgacttcc ggccatacgg
     1921 gcatcggggc attcctcaag gacattaccg caaaccgaag aaatgaagat tatctgctgc
     1981 agcagctcac ccgccacagc atctttgaac gcagtcttac catggacttt gatacgctcg
     2041 aagggcaatg tgattatgcg ctgcgacaag ccatgcatct gaccgagtgc cagtttggcg
     2101 tgattcacag ctatgcgtac gatgaaatga aatccggatc ggactcggtc aatcggataa
     2161 cgcgtgtcag ccataatgtc gaccaagcac tgctcacgga gcaactgaag gacctttggc
     2221 aggaaatgat aaaaagcccg caagccctca tcatgaatga tttcggcagc agtcactcgc
     2281 tccaactgcc tgccgggacc gccgggcatc cgatcatcaa caatgttttg tcggtgccgg
     2341 tattctttga acaccattta aaagtagtca tcatcctggc caacaaagag aacgggttca
     2401 gtgaattcga taagaatgaa gtctccctgt tgctgaacag tttttgtctg ctgtttgcct
     2461 accgccataa ccaggccctg accgaacggg aacgcaacaa attaaagagc attttcaacg
     2521 aactgccggc cctgatttgc gaattcctgc ccgacagtac gttaaccttc gccaatcaaa
     2581 gctattgcga ttatttcggt ttttacaacc aatccatggt gggcaggaag tttttggatt
     2641 taatcccggt ggaagaccgg gccggcgtgc tgcgcagcta tgccagtctg accccggaaa
     2701 accgcaccaa cgtgtatgtc cacaaagtgg aggcaaacgg ccaaatccgc ttgctggaat
     2761 ggcgggacat cggctttttc aacaaaatgg gggcccccgt ctactactac tcaatcggcc
     2821 ttgacgtcac cgaaaaaacc gctctggaag aagagcggga ccgcctgctg catcagtttg
     2881 aagccatgat caacaatcat gacgcggtca tgctgttgat tgagccgaat tccggcagga
     2941 tcctggactg caatccggcc gctgccgagt attacggtta caaccgggac gagctcgtgc
     3001 gaatgaagat cgaggagatc aacatgctgc ccggcgaaga agtgaaaatg ctgcgcctgc
     3061 aagcactgga aaagggacag cgctatttca ccttccccca ccgtttaaag aacggcgaca
     3121 tccgcaaggt ggacgtctat gcttcgccca tcctgtacaa tggccaaaaa ttattgtttt
     3181 ccattatttt cgatgtcacc gaaaaagaaa atgcctatgc ggaaatccgc tacatcagtt
     3241 accacgatta tctgaccggt ctgtacaacc ggcgcttttt tgaggaagaa ttcgcccgct
     3301 tgaatacgga tgaacatttc ccgctggcca tcatcatggg cgacgtcaac ggcatgaaat
     3361 tgatcaatga tgcgctgggc aaccaggccg gtgaccaggt gctgacggat gccgccgaat
     3421 tgctgcagcg tttcgcccgg gaacaggata tcgtcgcccg cgtgggcggg gatgagtttg
     3481 ccgtcattct gccggggtgt aacgaaacac aagcgcgcag ccgggtcaag caaatcgaag
     3541 cccaggccca ggaaaaccgc cagctaacct cacctgacaa tcttttgtcg atttccttcg
     3601 gctatgccgt gcagaagcgt ccggggcaga ccctggatgt tctgatgaag gaagcggaag
     3661 gtttcatgta tgccaacaag tactatgatg agcgcagtat caagggaaaa acgattgcga
     3721 ttatcatgaa tacgctgttt gagaaaagcc ctagggagaa aatgcattcc gagcgggtcg
     3781 gcaagctggc ggcggcgatt
//
LOCUS       DAOTHZ010000138         3747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_29046_length_3747_cov_7.649512, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000138 DAOTHZ010000000
VERSION     DAOTHZ010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29046_length_3747_cov_7.649512"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..686)
                     /locus_tag="P5549_10295"
     CDS             complement(27..686)
                     /locus_tag="P5549_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRW13500.1"
                     /translation="MEYNLFISLLDLIIHLDKHLIYLINTYGVLVYAIIFLIIFCETG
                     LVVTPFLPGDSLIFVLGALGASGQINLPAICVILMTAAILGNMTNYQIGRFIGPKIFE
                     RDSGLFFKKEYLIRTHAFFEKHGGKTIVIARFMPIIRTFAPFVAGIGEMDYARFTFFN
                     LIGCVAWVAIFLIGGYLFGNVPGVQNNFTLVIFAIIFVSLIPAIVVSWRQKRASTGKE
                     Q"
     gene            998..2929
                     /locus_tag="P5549_10300"
     CDS             998..2929
                     /locus_tag="P5549_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRW13501.1"
                     /translation="MTFTHLFSPARIGHLTLKNRLVMAPMATGFADASGEVSDMMVAY
                     YRARACGGVGMVIVEAACIDPPVGLESPHQLNIDQPACLTGLQRLAEEIKLAGARAFI
                     QLFHAGRQGSSLMNGGAQIVAPSAVACRISREVPRELTCDEIHFLRDKFISSAFYAFQ
                     AGFDGVELHAAHGYLLNEFLSPHTNQRRDEYGGNPENRMRLLLEIVAGIKELLPHLAV
                     SVRLNIDDFVNGGLTMKESLVICEALQSAGADLIHCSSGTYESGLKSIEPASYPQGWR
                     IYLAEAVKDTVSIPVMGGGMIRDPQLAEQIIAENKADFVFLGRPLLADPQWPLKVKQN
                     RLDDIRPCINCNHCISLNFNGLAIRCTVNPYTGRENQQNTSLLPDREHRAVVVGAGPA
                     GMQAALSLQQRGIQVRLYEQTERLGGALNLAMIPPHKERIGLLRDYLIRSLEQSPVEI
                     YLQREFTLADLQETAPDILVIATGSRSDCPTSGLDENGISWEAKRVLKDQVDWSDTYI
                     AVIGGSSTGCETAEYLSRLGNRIVLIEKDKILARGMEKKNRRDLLTRLDHLDVQRKTG
                     CSFKKMLDHKLIIETQEGLEEVLTVDKVVWACGSLPNNHLYYEVIDKVPRVFLIGDAN
                     QVRGFAEAISEGAAVADLF"
     gene            2964..3632
                     /locus_tag="P5549_10305"
     CDS             2964..3632
                     /locus_tag="P5549_10305"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRW13502.1"
                     /translation="MKKKPEETTRLEELKEFEYRAYQEGFQTIAGTDEVGRGPLAGPV
                     AAAAVILPQDFFLEGVDDSKRLSDKKRRKLVDDIKKEAVSWAVALLSPQYIDRENILQ
                     ASREAMRLAVQALRPAPDFLLVDAIKIPDLPIQQLPIIKGDSLSVSIACASIIAKVER
                     DETMLAYDRLYPQYGLGKHKGYATREHLLALQEFGPCPLHRRSFEPVKSMLKSSAGEQ
                     ITLF"
BASE COUNT         1021 a          904 c          971 g          851 t
ORIGIN      
        1 ttaacatgat tgtcaatata ctcaatttat tgctccttgc cggtgctggc tcgcttctgc
       61 cgccaggaaa ctacgattgc cgggatcagt gagacaaaaa tgatggcgaa aatcaccagc
      121 gtaaaattat tctgcacgcc gggaacattg ccgaacagat atccgccgat caggaagatc
      181 gcaacccagg ccacacagcc aattaggtta aaaaacgtaa agcgggcgta atccatttca
      241 ccgatcccgg ccacaaaagg ggcgaatgtt ctgatgatag gcataaaacg ggcaataaca
      301 atcgttttgc caccgtgttt ttcaaaaaag gcatgggtgc ggatcaagta ttcctttttg
      361 aaaaacagtc cagaatctct ttcgaatatt ttcgggccga taaaacgacc gatttgataa
      421 ttggtcatat ttcccagaat ggcggcggtc atcaggatca cacagatcgc cggcagattg
      481 atttgtccgc tggcccccag ggcacccagc acaaagatca acgaatcgcc tggcagaaaa
      541 ggggtaacga ccagaccggt ttcacagaaa atgatcaaaa aaatgatcgc ataaaccaac
      601 acgccatagg tgttaattaa ataaataaga tgtttgtcca aatgaatgat caaatccagc
      661 aagcttataa ataggttata ttccataatc aattcctttt tggtaattaa tacctgaaaa
      721 cctccaagtt ttcagagcca accagcgata gcaataatac gtcatctggt tatagcattc
      781 acagaggacg cgggcaatcc ttttaaagtt aaaaattgcc tttcctgtcc tggactgact
      841 tatatagtat acaacaactg gtggctcaga caaaataggt aattaccagg cagagcgaaa
      901 aaaacaatta attcatccaa ataaaaggaa gatcaggagt ggaaaaggaa tatatcaata
      961 gaaataatta tcacggtgtt caagggagaa tcacttgatg acttttactc atttattcag
     1021 tccggcaaga atcggccatc tcacccttaa aaaccgcctc gtgatggcgc cgatggctac
     1081 tggctttgcg gatgccagcg gcgaagtcag cgatatgatg gtggcctact accgggcccg
     1141 cgcctgtggc ggagtcggga tggtgatcgt ggaggcagct tgtatcgacc cgccggtcgg
     1201 gctggaaagt ccacatcaac tcaatattga tcagcccgcc tgtctgaccg gtttgcagcg
     1261 tctggcagaa gaaatcaaac tggccggcgc acgcgcgttt attcagttgt ttcatgccgg
     1321 gcggcaggga tcgtcactga tgaatggcgg tgcccagatc gttgcgccct cagcggttgc
     1381 ttgccggatc agtcgggaag tgccgcggga attgacctgc gatgagattc attttttgcg
     1441 ggataaattt atctcctccg ctttttatgc tttccaggcc gggtttgacg gcgtggaatt
     1501 acatgcagcg catggctatt tgctcaacga atttctgtcc ccacatacca accagcgccg
     1561 ggacgaatat ggcggcaatc cggaaaatcg tatgcggctg ctgctggaga ttgttgccgg
     1621 gatcaaagaa ttactgcccc atttggccgt ttctgtacgg ctcaatatcg acgattttgt
     1681 caacggcggg ctgacgatga aagagagcct ggtgatctgc gaggcgctgc aatcagccgg
     1741 tgccgacctt attcattgct cgtcgggaac atatgaatct ggcttaaaaa gtattgaacc
     1801 ggcctcctat ccgcaaggct ggcgaattta tctggccgaa gcggttaaag ataccgtttc
     1861 cattccagtg atgggcggcg gcatgattag agacccgcag ctggctgaac aaatcattgc
     1921 agagaacaaa gccgactttg tattcctggg ccggcccctg ctggccgatc cccaatggcc
     1981 gctgaaggtc aaacaaaacc ggttggatga tatccgcccc tgtatcaact gcaatcattg
     2041 tatcagtctg aattttaacg gcctggcaat ccgctgcacg gtcaacccgt atacagggcg
     2101 ggaaaaccag caaaatacca gtctgctgcc tgacagagaa catcgcgccg tcgtcgtggg
     2161 cgccggaccg gccgggatgc aggcggcact ttccctgcag cagcggggga ttcaggtcag
     2221 gctgtatgaa caaacggagc gactgggggg cgcactcaat ctggccatga tccctcccca
     2281 caaagaaaga atcggcttgc tgcgtgacta tttgatcaga tctctggaac aaagcccggt
     2341 ggaaatttat ttacagcgag agttcaccct cgccgatcta caggagacgg cccctgacat
     2401 tctggtcatt gcgaccgggt cgcgcagtga ctgtccgact tccggtctcg atgaaaacgg
     2461 gatcagttgg gaagccaaac gagtattgaa agatcaggtt gattggtcag atacgtatat
     2521 tgctgtgatt ggcggaagca gcaccggatg tgaaaccgct gaatatttaa gccggcttgg
     2581 taatcgcatt gtactgattg aaaaagacaa gatcctggcg cgggggatgg aaaaaaagaa
     2641 ccgtcgtgat ctcctgaccc gtttggatca cctggatgta cagagaaaaa ccggctgcag
     2701 ctttaaaaaa atgctggatc ataaactgat tattgaaacc caggaaggac tggaagaagt
     2761 tctgacggta gacaaagtgg tatgggcttg tggatcatta cccaacaacc atttgtatta
     2821 tgaagtcatc gacaaagtgc cccgggtgtt tttgatcggc gatgccaacc aggttagagg
     2881 ctttgccgag gccatcagcg aaggggcagc ggtggcagac ctattctaaa atattcaata
     2941 ggacggagaa accattggtc aaagtgaaaa aaaaacctga ggaaacaacc agactggaag
     3001 aactgaaaga gtttgaatat cgggcttacc aagaaggctt tcagacgatt gccggcacgg
     3061 acgaggtggg gagaggacca ctggctgggc cggtcgcggc tgccgccgtc attttgccgc
     3121 aggatttttt cctggaaggc gtggatgatt caaaacgttt gagtgacaaa aagcggcgca
     3181 agctggtcga tgatattaaa aaagaggcag tcagctgggc cgtagccttg ctttctccac
     3241 aatatataga ccgcgaaaac atactccagg ccagccggga agctatgcgt ctggctgtgc
     3301 aggctttgcg acctgccccc gactttttgc tggtggatgc tatcaagatt cctgatctgc
     3361 ccattcagca actgcccatt atcaaaggag acagccttag cgtatccatc gcctgtgcat
     3421 ccatcatcgc caaagtggaa cgagatgaga ccatgctcgc ctacgatcgc ctttatcccc
     3481 aatacggact gggcaagcac aaaggatatg ccacccgcga acatttactg gcattgcagg
     3541 aattcggtcc ctgtcccctc catcgccgta gttttgaacc ggtcaaatcc atgctgaaaa
     3601 gcagtgccgg cgagcaaatc accttattct gacaggggga tcaagtgaga tgagaatcgc
     3661 cttgggccga cagggagaag atatcgccgc tgcctacctg cagcagcagg gttatgaaat
     3721 actgacccgc aactaccgtt gccgcta
//
LOCUS       DAOTHZ010000139         3721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_29330_length_3721_cov_7.219858, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000139 DAOTHZ010000000
VERSION     DAOTHZ010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29330_length_3721_cov_7.219858"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1713)
                     /locus_tag="P5549_10310"
     CDS             complement(40..1713)
                     /locus_tag="P5549_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW13503.1"
                     /translation="MSSVYETRFWLKNFEWKTPHSINYPKMPAYGVLRNASVYKPDQA
                     ATWFYGAEISYNDLYLKVTRLANVLVENGVQKGDRVGLLMPNCPQAVIAFWAILMAGG
                     IVVNLNVLYTKDELSFLLNDTAPKALFTYDAILPLVKAINDTSKNPIKTVYVTKLTDF
                     MPGPQVSTAAELGLEEGYLHFSEVLETDKRWAPPLVDIKHDDPALIQYTGGTTGSPKG
                     AVLTQYNIVAASFIVVRWCSDFVDSHEYERRKVLSILPYSHIYGEVCGILYSTFQCAT
                     MVILPRWDTDEVFNILSAHEHLSYFPSVPTMLQALFYSPRVDEIDWHRKIGYCGSGAA
                     PAPLALIQRCRSKDFNFCEGWGMSETCSMGAGTPNANSFKPNSIGIPMIDIDIRIVGE
                     DGNDVPLGEQGEIWIKGPIVMKEYWNRPEDTDAAFNDGWLRTGDVAYMDEDGYIFIVD
                     RLKDLIIAGGFNIYPRDVDEVLLKHPKVADAMVIGVPDEYRGETVKAFVQLMPGETAT
                     AEELIAFCRESLAAYKVPYQVEFRDALPRTATGKALRRILKEEEAQKNK"
     gene            complement(1847..3676)
                     /locus_tag="P5549_10315"
     CDS             complement(1847..3676)
                     /locus_tag="P5549_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRW13504.1"
                     /translation="MASNFIYSNRDLKFIVKEWLGTERLLGFEKFRDYYGMDDVDVIL
                     DQGLKIVTEQIAPTNEDGDTLHAQFKDGTVTVPPSFHPIYKFINEEGWGIGNLLEEGE
                     GTLPQILYSSFIEMITSANPAWIPYLNLSGGVLELIQAYGREKDKEVFLPPLCSGEWS
                     GTMCLTEAVGGSDVGDILSKAYPTDEPGIYKIKGTKIFITAGDHDICSNIIHMVLARI
                     EGARPGTKGLSLFIVPKFRVNEDGSLGEPNDVITVGIEHKLGLKGSSTASLSFGENNS
                     CLGILMGEPPLDGMGEGMAQMFQMMNGARMDTGMAGYCVATQAYNNAAEYAKIRVQGR
                     SFKDPKAGRVPIIKHADIRRILLNIKSCTDAMRAMAFKTYFYFDLMRHSPDEAERQMA
                     RDRIEVMTPLVKAYSTDMAWPLIGDAIQVYGGYGFIEEYPVAHFARDSKIYSLWEGTN
                     FIQSQDLVGRKWTLNKGRLFQDWLDDLEGMLHAREGREEFKTEYAIMQAAFASYKRIK
                     ASMDEFGQTGKGEMLPLYATRLLHATSQLCCGTLILDQAAIAMDKINELGQDHWDFAF
                     YQGKIDGARFYIKNTVPSIMQLEVILQYADDSALLIPEESLGV"
BASE COUNT          899 a          937 c          938 g          947 t
ORIGIN      
        1 taacgggaga tggtctcggt ggggtttcaa caaagaggat tatttattct tttgtgcttc
       61 ttcttctttg aggattctgc gcaaggcttt gccggttgcg gttcgaggca gggcatcccg
      121 gaattcaacc tggtagggga ccttgtaggc ggccaaagac tcccgacaga aagcaatcag
      181 ctcttcagcg gtcgccgtct cacccggcat cagttggaca aaagccttga cggtttcacc
      241 gcgatattca tccggaacac cgatgaccat ggcatcagcg actttgggat gtttcagcag
      301 gacttcatcg acatcacgcg gatagatgtt aaacccaccg gcaatgatta agtcctttag
      361 ccggtcaacg atgaagatat agccgtcttc atccatgtag gccacatcac cggtgcgcag
      421 ccagccgtca ttgaaggcgg catcagtgtc ttccggccgg ttccagtatt ctttcatgac
      481 aatcgggcct ttgatccaga tctcgccttg ttcacccagg ggaacgtcat tgccgtcctc
      541 gccgacgatg cgaatgtcga tatcgatcat ggggataccg atactgttgg gtttgaaact
      601 gttggcgttg ggagtgcctg cacccatgga gcaggtctcg ctcatacccc agccttcaca
      661 gaagttaaaa tccttggaac ggcatctctg aatcagtgcc agcggcgcag gggcagcccc
      721 ggaaccgcaa tagccaattt tgcggtgcca gtcgatttca tcgacgcggg ggctgtagaa
      781 aagtgcctgc agcatcgttg gtacggaggg gaagtaagag aggtgctcat gcgcactcag
      841 aatgttaaat acttcatcgg tgtcccaacg cggcaagatg accatcgtgg cacactggaa
      901 ggtggaataa aggatgccac agacttcgcc ataaatgtgt gaatagggca ggatgctgag
      961 aactttgcgg cgttcatact catggctgtc aacaaaatcg gaacaccatc tgaccacgat
     1021 aaaggaggct gctacgatat tatattgggt taaaacagct cccttggggc tgccggtggt
     1081 tccgccggta tactggataa gggcaggatc atcatgtttt atgtcaacca acggaggtgc
     1141 ccatctttta tctgtttcca acacttcgct aaagtgcaga taaccttctt caaggcctag
     1201 ttcagcggcg gtgcttactt ggggaccggg cataaaatcc gtcaatttgg ttacgtaaac
     1261 tgtcttaatc gggtttttgc tggtgtcgtt gatcgctttg accaacggca gaatggcatc
     1321 ataggtaaac aaagcttttg gagccgtatc attcagcagg aaggatagct catctttggt
     1381 atacaggaca ttgagattta caacgattcc gcctgccata aggattgccc agaaggcgat
     1441 gacggcctgg ggacagttgg gcatcagcaa tcctacccga tcaccttttt gcacgccgtt
     1501 ttccaccaat acgttggcca ggcgggtgac tttgaggtac aggtcattgt aggatatttc
     1561 tgcgccatag aaccaggtgg cggcctggtc aggtttgtaa acactggcgt ttcttaatac
     1621 gccgtaggct ggcattttag gatagttgat gctatggggt gttttccatt caaaattctt
     1681 gagccaaaaa cgggtttcat aaacagacga catgatatca ctcctcttcc atacagtaaa
     1741 tcgaccaggc aacaaacaaa actccagcgc aaacctccga cagccaatga aacagggtat
     1801 aacagcgtgg atgcaatccg gttatcttgc cgtttataac cggcgcttat acccctaatg
     1861 attcttccgg aatcaataac gctgagtcat ccgcgtattg caatatcacc tccaattgca
     1921 tgatgctggg aacggtgttc ttaatataga agcgtgctcc atcaatcttg ccttgataga
     1981 aggcaaaatc ccaatgatct tgtcccaatt cgttgatttt atccatggca atggcagcct
     2041 ggtccaggat cagggtaccg cagcaaagct gagaggtagc atgcagcaac cgcgtcgcgt
     2101 acagcggcag catttcgcct ttgccggttt gtccaaactc gtccatgctc gccttgattc
     2161 gtttatagga ggcgaaggcg gcttgcatga tggcgtactc tgttttaaat tcctcccggc
     2221 cttcccgggc gtgcagcatc ccttccaggt catccagcca atcctggaag agccggcctt
     2281 tattcagtgt ccattttctt cccaccaggt cctgggattg aatgaagttg gtgccttccc
     2341 acaaagagta aatcttgctg tcacgggcaa agtgggccac cggatattct tcaataaaac
     2401 catacccgcc atagacctgg atggcatcgc cgatcaacgg ccaggccata tcggtggagt
     2461 aggccttaac caggggggtc atcacttcaa ttcgatcccg cgccatttgc cgttcagctt
     2521 catcggggct gtgccgcatg aggtcgaaat agaaataggt tttaaaggcc atggcgcgca
     2581 tggcatccgt acaagatttg atattcagca ggatgcgtcg gatgtcggca tgtttgataa
     2641 tcggcacccg cccggctttg gggtctttga aggaacgtcc ctgaacccgt attttggcgt
     2701 attcagctgc attgttatag gcctgggtgg cgacacaata tcctgccatg ccggtgtcca
     2761 tacgggcacc gttcatcatc tggaacatct gggccatccc ttctcccatt ccatccaggg
     2821 gaggctcacc catcagtatg ccgagacagc tgttgttctc tccgaagcta agcgaagcgg
     2881 ttgaagaacc tttcaggccc agcttgtgtt cgataccaac ggtgataaca tcattgggct
     2941 cacccagact gccatcttcg ttgacccgga attttggcac gataaagaga gaaagacctt
     3001 tggtgccagg acgtgcgccc tcaatccgag ccagcaccat atgaatgata ttcgagcaaa
     3061 tgtcatggtc accggcggtg atgaatattt tggtgccttt gattttgtaa attcccggtt
     3121 cgtccgtggg ataagccttg gataaaatat cgcccacgtc tgagccgcca acggcttcgg
     3181 tcaggcacat ggtgccgctc cattctccgc tgcacagagg aggaagaaaa acctccttgt
     3241 ctttttcccg gccgtaggcc tgaattaatt ccaatacacc gccgctcagg ttaagatagg
     3301 ggatccaggc cggattggca gaggtaatca tttcaataaa ggaagagtat aggatttggg
     3361 gcaaggtgcc ttcgccttct tccaacagat tgccaatccc ccagccctct tcattgatga
     3421 atttatagat gggatggaaa gatggcggaa cggtaacggt accatcttta aactgggcgt
     3481 ggagcgtatc accgtcttcg ttggtggggg cgatttgttc agtgacgatt ttcaagccct
     3541 gatccagaat tacatcgaca tcgtccatac cataatagtc acgaaatttt tcaaatccca
     3601 acaacctttc ggttccgagc cactccttga caataaactt caagtcacgg tttgaataaa
     3661 taaaattgct ggccaccgtt aagcaacctc ctttgtcatt gttttataaa agttttccca
     3721 t
//
LOCUS       DAOTHZ010000140         3599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_30611_length_3599_cov_6.589729, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000140 DAOTHZ010000000
VERSION     DAOTHZ010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30611_length_3599_cov_6.589729"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1565
                     /locus_tag="P5549_10320"
     CDS             339..1565
                     /locus_tag="P5549_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRW13505.1"
                     /translation="MDTMTKLEILAGAAKYDVSCSSSGVSRNNQGGIGNAKSFGICHA
                     WSSDGRCISLLKILLSNDCIYDCRYCVNRRSNDIRRASFLPQELADLTIQFYRRNYIE
                     GLFLSAAVEISPDYTMEKIILVLTLLRREYSFQGYIHVKVIPGADPALIQQAGLLADR
                     MSVNIEQPTEQSLQLLAPQKKLPVLYASMAQLKEQIQIHKEDRRHLRHAPRFVPAGQS
                     TQMIVGASADSDLTILQTTQSLYQRFNMKRVYFSAYVPVNEDSRLPALATAPPLLREH
                     RLYQSDWLLRFYHFKAEEILDPVQAYLDTELDPKTAWAIRHLELFPVEINRASYEELL
                     RIPGIGPKSAQRIISQRRVAAVSVDELKKLGAVFKRAQYFVTCKGVYPGHHPLDPMTI
                     RRIMVPEPPTVQLSLF"
     gene            1663..2448
                     /locus_tag="P5549_10325"
     CDS             1663..2448
                     /locus_tag="P5549_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HRW13506.1"
                     /translation="MDYLYDGSFEGLLTCIYYHYYREKASGIYQQEYYQPALMTDSTE
                     VITDPMLAARVSTAIEQKISKTSLDLVYRVFVSSAANKEFLILHYLRWGFRLGAKVDL
                     YYSHPDVYPLQKIAHKVTAEVHRFLGLLRFADAGNFLYAVLSPDHYILPLIADHFADR
                     LAGERWIIHDRKRDLAVVYDGHHAAEDSARYQRQWYITDFPHPGPLLFTQKEQYYQKL
                     WQLYFDKIGIESRCNPRLQAQFVPYRYRGDLVEFKPIFIKNDG"
     gene            2954..3412
                     /locus_tag="P5549_10330"
     CDS             2954..3412
                     /locus_tag="P5549_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13507.1"
                     /translation="MGSSYNVCCRECGYTKPFMIGIGMMYDPHMLEDEDSASKFLPSI
                     IRSKKAVAQIRALLQEKAAVIAVDYGHNVYRCTKCGEFYGRFFIHLDYDGGSYEVDYK
                     CTKCRTSLELVGNDAGHRMGEVAIDLQKYPCPQCGKHSLYEDTESMILWD"
BASE COUNT          937 a          838 c          846 g          978 t
ORIGIN      
        1 ttgttgccga tttcacatga tagatttacc tggttttgat tctttttggt atctagtatc
       61 aagcgtctta tgttggcgga gcatttatgc cagggcgctt gatttgcttg ggttcaagat
      121 tcctttcaca gccgctgata cagttgtgaa tcgtagcgtc cgacaaggtc gttaacaatc
      181 actagaattc agacatcatc agcaaaatta ttcagaaaaa tgttcaataa gtttagtttg
      241 caatgaaatc aagcagtcat tgtgcagaac acttgtttgc accatgccta ttctatgcta
      301 tactgaattt gccaatcatt tcatctgagg tgaatcgaat ggataccatg accaaactcg
      361 agattctggc gggagcagcg aaatacgatg tatcttgttc ttccagcgga gtttcccgaa
      421 ataatcaggg cggtatcggc aacgccaaat cgtttggcat ctgtcatgcc tggtcgtcgg
      481 atggacgctg tatttccctg ttaaaaatcc ttttaagcaa tgattgtatc tatgattgcc
      541 gttattgtgt caaccggcgt tccaatgata ttcgccgggc cagctttttg ccgcaggaac
      601 tggctgattt gaccattcag ttttaccgcc gcaattatat cgaaggcctg ttcctgagtg
      661 ccgcggtgga gatatcgccg gattacacca tggaaaaaat cattttggtt ctgaccttgc
      721 tgcgccggga atacagcttt cagggctaca tccatgtaaa agtcattccg ggcgccgacc
      781 cggccttgat tcagcaagcc ggtctgctgg ccgaccgaat gagcgtcaac atcgaacagc
      841 ccacggaaca aagcctgcag ctgctggctc cgcagaaaaa gctcccggta ttgtatgctt
      901 ccatggcgca actgaaggaa caaattcaga tccacaagga agatcgcaga cacttgcggc
      961 atgcgcctcg ttttgttccg gccgggcaat cgacccagat gattgtgggc gcctcggcgg
     1021 acagcgattt gaccattttg cagaccaccc agtctttata ccagcgcttt aacatgaaaa
     1081 gagtctattt ttccgcctac gtgccggtca atgaagactc ccgcctgccg gccctggcaa
     1141 ccgcgccgcc gctgctgcgg gaacatcgct tgtaccaatc cgattggctg ctgcgctttt
     1201 accattttaa agccgaagaa attctggatc cggtgcaagc ctacctggac actgagctgg
     1261 accccaaaac cgcctgggcg attcgtcatc tggaattgtt tccggtggaa atcaaccggg
     1321 cttcctatga agaactgctg cgaattccgg ggatcgggcc taaatcggct caacgcatca
     1381 tcagccagcg tcgggtggct gccgtttccg tggatgaatt aaagaaattg ggggctgtct
     1441 ttaagcgggc gcaatatttc gttacctgca agggcgtcta tcccggccat catccgctcg
     1501 atcccatgac catccgccgg attatggtgc cagagccgcc aaccgtgcaa ttgagtctgt
     1561 tttgacaagc aattgctgaa gccaaacttt aaaggataca ctgtatcatt tgcattctgg
     1621 tttttaaccg aaaattgcga agccaactca gggggagatt tgatggatta cttatatgat
     1681 ggcagttttg aggggctatt gacctgcatc tattatcatt attatagaga aaaagccagc
     1741 ggcatttacc agcaggagta ctaccaaccg gccctgatga cagacagcac cgaggtaatc
     1801 actgacccca tgctggcggc gcgggtatcc accgcgatcg aacaaaaaat ctccaagacg
     1861 tctctggatt tggtttatcg tgtctttgtt tcttccgctg ccaacaagga atttctcatt
     1921 ctccattacc ttcgttgggg atttcgcttg ggggccaagg tcgatcttta ttattcccat
     1981 cccgatgttt acccgctgca aaagattgcc cacaaggtaa ccgctgaagt ccatcgcttc
     2041 ctgggattgc tgcggtttgc cgatgcggga aattttttat atgcggtttt gtctcccgat
     2101 cattatatcc tgcccctgat cgccgatcat tttgccgatc gtctggccgg cgaacgctgg
     2161 atcattcacg accgcaaaag agacctggcg gtggtttatg acggacatca tgccgctgaa
     2221 gactctgctc gttaccaacg ccaatggtat atcactgact tccctcaccc cggaccactc
     2281 ctcttcaccc aaaaagagca gtactaccag aaactgtggc agctctactt cgataaaatc
     2341 ggcattgagt cccggtgcaa tccccgcctg caggcccaat tcgttcctta tcgctaccgc
     2401 ggcgatctgg tcgagtttaa accgattttc ataaaaaatg atggttaagc tcaggaccac
     2461 ggattgaaag cgctgtcaga aactccgcct ccgaacattt ataaaaagtg aatgtaggaa
     2521 aaaagcggcg atttttttcc tacgttatgt ttacatagga aaaaaatcag tttaaggata
     2581 aataccgaag tagacgccat gactgcagaa ataaaagaaa agtagtcaaa gatttactaa
     2641 aaggagctta taatcttaat gaatgaaccg tcccgggcat gacgttaaac ttccccggcc
     2701 ttaggccatt tttatgatta cctaccttga tactcctctg cgtctctccc aaatgcagga
     2761 attttgtaag attaaataga agaatagcaa cgatggtttt gcagaaaaca cattacgtta
     2821 aatatttgat tacaccttct gctgacatgt tttaaatagc cggaagtcac caggatcagg
     2881 ttcagtttat tgacatatag gtgtcactaa aatctggcac agtaagtttg tacaaataaa
     2941 gcgaggtgat ctgatgggca gttcatacaa tgtctgctgc agagaatgcg gatatacaaa
     3001 gccctttatg attggaatcg gcatgatgta cgatccacat atgctcgaag acgaggattc
     3061 agcctctaaa tttcttcctt ccattattcg ctccaagaaa gcggttgcac agataagagc
     3121 attgttacag gaaaaagccg ctgtgattgc cgttgattac ggtcataatg tgtaccgttg
     3181 tacgaaatgc ggagaattct atggccgatt cttcattcat ctggattatg atggcggcag
     3241 ttatgaagtc gattacaaat gcacaaaatg cagaacctct cttgagcttg ttggcaatga
     3301 tgccgggcac agaatggggg aggtcgccat tgatttgcag aagtatccct gcccgcaatg
     3361 cggaaagcat tccctttatg aagacacaga aagtatgatt ttatgggatt gagggtatgt
     3421 tgcttccggg gcatgttcaa tgaatctatg gtttgaggtg aatataattt gataaatatt
     3481 gaattggtat ggcctgattg caatagcttt ggcctgtata ataacggcgg agcagacgat
     3541 gtatttttgg ttgaaggcag atacgacgaa ttaaccgata tgatcaggtc tcattttgg
//
LOCUS       DAOTHZ010000141         3575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_30906_length_3575_cov_7.281534, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000141 DAOTHZ010000000
VERSION     DAOTHZ010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30906_length_3575_cov_7.281534"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..1899
                     /gene="dnaG"
                     /locus_tag="P5549_10335"
     CDS             127..1899
                     /gene="dnaG"
                     /locus_tag="P5549_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRW13508.1"
                     /translation="MGYYSDDQLIREIESKIDIVELISESVSLNRKGNRYWGLCPFHQ
                     EKTPSFSVSRDKQMFYCFGCHAGGNVFSYLMKHESLEFKEALEVLAAKAGVELTTNKS
                     QKNQDLHKQMIAVNNAAAVYFHQQLQSARGKPAREYLAQRGICPDTMEMFNLGYAPNE
                     WQALTDYLLKKGYSLDLMKNSGLIKRNETQERYYDLFRDRIIFPITHYSRDVIGFGGR
                     AMGDAMPKYLNTAETDIYSKRKNLYGLTQAREEIRNKNEVILVEGYMDCIKLQQSGIR
                     NTVASLGTSLTEEQASLLHRYAEKAIVLYDGDEAGQRETMRAIEILRRQDLRVEVIVL
                     PVGKDPDDFIEKYGKEGFLHYTQNNTYSHIEFKLNRLINDCPRLDLDAKTSIIRQLKG
                     EIKALNSIVEQEYFINILARKLKLEEIVVAQEIRSGRTAAARNKTGIIRDNNKYGKYG
                     IQEKILAAMLSNENTFAKVNERIGINFFANPDYNTLANIYEQLQEGKENKMHEMSRIA
                     AEEDLEAVFARISMIMDELDVNDREVEEFIRRVEIKKSEAAWNKVMKRIDMLGESGDF
                     DRVLTFILHLDKFINANPEGGIIQ"
     gene            1896..2975
                     /gene="rpoD"
                     /locus_tag="P5549_10340"
     CDS             1896..2975
                     /gene="rpoD"
                     /locus_tag="P5549_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRW13509.1"
                     /translation="MKSDRKLAETIVILAEKGKKKKHLSYEEIIDELQNYQLEPDNIE
                     DIFEHLQSLGISVGPDVELESEDIESENIETEEVIVPDGIEIDDPVRMYLKEIGRVRL
                     LTADEEIDLATRIEGGEEEAKRQLVEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLM
                     KAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLSRVQRSLL
                     QTLGREPTPQEVAEEMDIPVDRVIEIMKVAQEPVSLETPIGEEDDSHLGDFITDEEAE
                     SPEESASFVLLREHMDGILNTLTDREEKVLRLRFGLDDGRPRTLEEVGQEFGVTRERI
                     RQIEAKALRKLRHPSRSKRLKDYIE"
     gene            3046..3120
                     /locus_tag="P5549_10345"
     tRNA            3046..3120
                     /locus_tag="P5549_10345"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3078..3080,aa:Asn,seq:gtt)
     gene            3276..3351
                     /locus_tag="P5549_10350"
     tRNA            3276..3351
                     /locus_tag="P5549_10350"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3309..3311,aa:Ile,seq:cat)
     gene            3501..3575
                     /locus_tag="P5549_10355"
     tRNA            3501..3575
                     /locus_tag="P5549_10355"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3534..3536,aa:Glu,seq:ttc)
BASE COUNT         1068 a          758 c          921 g          828 t
ORIGIN      
        1 attcccatgt attgtacccg gaaacgatac gcgaagaata aacaaaactt ataaattatt
       61 atattttgac aaaattcaat tattaaagca ggtatttcgt tttaagtata gaaatgatgg
      121 tgagtaatgg gatattattc tgatgatcag cttatacgcg aaatagaatc aaaaatcgac
      181 attgtggaac ttatttccga gagtgtaagt cttaatcgta aaggcaatcg atactgggga
      241 ctatgtccgt ttcaccagga gaaaacaccc tcttttagtg tcagtcggga taaacaaatg
      301 ttttactgct ttggttgtca cgcggggggc aatgtatttt cctatttgat gaaacacgaa
      361 agtctggagt tcaaagaagc cctggaagtg ctggcggcca aagccggggt tgagctgacg
      421 actaacaaga gccaaaaaaa ccaagatctg cacaagcaga tgatcgcggt caacaatgct
      481 gcagctgttt attttcatca acagctgcaa tccgccaggg gaaaaccggc tcgggaatac
      541 ctggcccaac gcggtatttg cccggatacc atggagatgt ttaatctggg ttatgccccc
      601 aatgaatggc aggctctgac ggattatttg ctaaaaaaag gatactccct ggatctgatg
      661 aaaaactccg gattaatcaa acgcaatgaa acccaggaac ggtattatga tttgttccgc
      721 gatcgcatca tttttccgat tacccattac agccgggatg tgattggatt tggcgggcgg
      781 gcgatgggag atgccatgcc caagtatctc aacacagccg agacagacat ctactccaaa
      841 cgcaaaaacc tgtacggtct gacccaggcc cgggaagaga tccgcaacaa gaatgaagtg
      901 attctggtcg aaggctatat ggattgcatt aaattacagc agtccggcat acgcaacacc
      961 gtagcctctc tgggaacatc gctgaccgag gaacaggcct cactgctgca tcgttatgcg
     1021 gaaaaagcaa tcgtactcta tgacggagac gaagccgggc agcgagaaac gatgcgggcg
     1081 attgaaatat tgcgccggca ggatctgaga gtggaagtca tcgttttgcc ggtcggcaag
     1141 gatcccgatg attttattga aaaatatggc aaagagggat ttttgcatta tacacagaat
     1201 aatacctata gtcatattga atttaaattg aatcgcttga tcaatgactg ccctcgactt
     1261 gatctcgatg ccaaaaccag tatcatcagg cagttgaagg gagagatcaa ggcattaaac
     1321 agcattgtag aacaggagta ttttatcaac atcctggctc gcaaactgaa gttggaagaa
     1381 atcgtggtag cacaggaaat ccgctccggg cgaaccgcag ctgcaaggaa taaaactgga
     1441 attatcaggg ataataataa atacggaaaa tatggaattc aggaaaaaat cctggcagcg
     1501 atgctcagca atgaaaatac atttgccaaa gtcaacgaaa gaatcggaat caacttcttt
     1561 gccaacccgg attacaatac cctggccaac atatatgaac aactgcagga aggaaaagag
     1621 aacaagatgc atgaaatgag ccgaattgcg gctgaggaag atctggaggc agtgtttgcc
     1681 cggatctcca tgattatgga tgagctggat gtaaacgaca gagaagttga ggagtttatc
     1741 agacgggttg aaatcaagaa gagcgaagct gcttggaaca aagtcatgaa acgaatcgac
     1801 atgctgggtg aaagcggaga ctttgaccgt gtcctgactt ttatcctgca tttggacaaa
     1861 ttcatcaatg ccaacccgga aggggggatt atacaatgaa atctgaccgc aaattagccg
     1921 aaacgattgt tatactggcg gaaaaaggga agaagaaaaa gcacctcagt tatgaggaaa
     1981 ttattgacga attgcaaaac taccaactgg agccggataa catcgaagat atttttgagc
     2041 atttacagtc tctgggcata tctgtagggc ccgatgtgga gctggaaagt gaagatatcg
     2101 agtcggaaaa catagagacg gaagaagtca ttgttcccga tggcatagaa atagatgatc
     2161 cggtacgaat gtacctgaag gaaatcggcc gggtccgttt gctgactgcc gacgaggaaa
     2221 ttgacctggc cacccgcatc gaaggcgggg aagaagaagc caaacggcag ctggtagagg
     2281 ccaatttgcg cttggtggtc agtattgcca agcgatatgt gggccgcggc atgctttttc
     2341 tcgacctgat ccaggaaggc aatctgggtt tgatgaaagc agtggagaaa ttcgactacc
     2401 gcaaagggtt caaattcagc acctacgcca cctggtggat cagacaggcg attacccggg
     2461 ccattgccga ccaggcgcga accatcagaa taccggtgca tatggtggaa accatcaata
     2521 aattgtcccg tgtgcaaaga agtctgctgc agaccttggg ccgtgaacct actccccagg
     2581 aagtggcgga ggaaatggac attccggttg accgagtcat tgaaatcatg aaggtagcgc
     2641 aggaaccggt atctctggag acgcctatcg gtgaagaaga tgacagccat ctgggcgatt
     2701 ttatcactga tgaagaagca gaatcgccgg aggaatcagc ctcctttgtc ctcctccggg
     2761 agcatatgga cgggatcttg aataccctta ccgatcgtga ggaaaaggtt ctgcgactgc
     2821 gtttcggttt ggacgacgga cgtccccgca cgttggagga agtgggtcag gaattcggtg
     2881 taaccaggga aagaatcaga cagattgaag ccaaggcctt gcgaaaatta agacatccat
     2941 cccgcagcaa gcgactgaaa gattatattg agtagggcaa taaaaaaaac ttcggttatt
     3001 gacaagcacc ggccaatggt tatataataa ttctcgttgg gtccttcctc gatagctcaa
     3061 cggtagagca tccggctgtt aaccggaggg ttgtaggttc gaatcctact cggggagcca
     3121 ttatgttaat caggcggtaa ccctcaccgg ctaccgcttt ttattgccaa agaagtgctg
     3181 gagaccgccg gtgaaaatag cgattataaa gttctgctat tgacctttgt gactattttc
     3241 tatatactaa ctcttgcggg gtttcaagca tccccgggcc tatagctcag ttggtagagc
     3301 taccggctca taaccggttg gtcccaggtt cgagtcctgg taggcccacc atttttgaag
     3361 tcagaagcct ggcatcagaa gtcggaacaa aaaagccgga ttctgggacg gtttcctgat
     3421 atagaagtgt gaattcagag gttcataaaa gattcggttt tggaatccaa cctcttttct
     3481 ctgaaatcaa cttccctcat ggccccttgg tcaagcggtc aagacaccgc cctttcacgg
     3541 cggtaacagg ggttcgagtc ccctaggggt cacca
//
LOCUS       DAOTHZ010000142         3541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_31293_length_3541_cov_6.931440, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000142 DAOTHZ010000000
VERSION     DAOTHZ010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31293_length_3541_cov_6.931440"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="P5549_10360"
     CDS             complement(<1..356)
                     /locus_tag="P5549_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRW13510.1"
                     /translation="MEKIRLDALLCEKGLAVSREKARAMIMAREVQIEGKIIDKPGVK
                     ILPETEIVITAVRPPYVSRGGHKLAGAIRDFQLDFSGRTVLDVGASTGGYTDCALQHG
                     AVKVFALDVGYGQLDWT"
     gene            complement(343..2253)
                     /gene="dxs"
                     /locus_tag="P5549_10365"
     CDS             complement(343..2253)
                     /gene="dxs"
                     /locus_tag="P5549_10365"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRW13511.1"
                     /translation="MPDFKTINSPLDIKKMSIPALNELAAEIRQQLIHSVSRSGGHLA
                     ANLGVVELTMALHYVYDTPQDKIIWDVGHQSYVHKILTGRQTRLSTLRQYGGISGFPK
                     AEESPHDAFNTGHSSTSISAALGMALARDIQKEHYSVIAVTGDGALTGGMTYEALNNA
                     GNAGCDLTVVLNDNEMSISKNVGAMSSYLNRLRTDPSYARTKEEIEGVLSRIPGIGPN
                     LARAAGRFKDTVKYIMVPGIWFEELGFTYIGPVNGHNLEELTVVLSNVRKMKGPVLVH
                     VITQKGRGYQPALKDPDMFHGIGPFDIDTGQTIRKPLPSYTEVFGQFMVDKGRQDDKV
                     VAITAAMASGTGLKNFARKFPERFFDVGICEQHAVTMAAGMAKAGLKPVVSIYSTFLQ
                     RAYDQIVHDVALQNLPVIFAVDRAGLVGEDGPTHHGVFDLAYLRSIPNMTIMAPADEN
                     ELMDMLHTAFTIKGPAAIRYPRGIGAGVAIKPERCTLPIGRSVKLHEGDNVGIIAIGR
                     TVEMARELIAILAQQGIAASLLNARFIKPLDDEAIKALAVKTGKLVTIEDNCLQGGFG
                     AAVAELLADDHIQAEVLRLGIPDEFIEQGKVEILFNYLNLDPESMADTIMARWPDLLP
                     HTAWELRKIGEN"
     gene            complement(2258..2830)
                     /locus_tag="P5549_10370"
     CDS             complement(2258..2830)
                     /locus_tag="P5549_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13512.1"
                     /translation="MRDQFNRNNLIAALWVAFFTFFIALLVSVGSQALVNAMHNVWIA
                     MIFLLLIIALGIFFDIIGTAAAAATLAPFNARAAKKITGASQAVRITKNASVVANYCN
                     DVIGDIAGTLSGAIGAAIVFQLVDSFSAPDTVLSGAVMTSLIAALTVGGKAVGKSIAI
                     ENANTIIFRVALIMAWWEKLTGIEFFKGGR"
BASE COUNT          844 a          994 c          851 g          852 t
ORIGIN      
        1 gtccaatcca gttgtccata accgacatca agcgcaaata ctttcacggc cccgtgctgc
       61 agggcacaat ccgtgtatcc tccagtggaa gcccccacgt caagcacagt ccgcccgctg
      121 aaatccagtt ggaaatcgcg gatcgctcct gccagcttgt gtcctccccg gctcacatag
      181 ggcgggcgga cagcggtaat aacgatctcg gtttccggca ggattttcac gccgggtttg
      241 tctatgattt ttccttcgat ctgcacttct ctggccataa tcatggcccg cgccttttcc
      301 cggctgaccg ccaggccttt ttcgcagagc agcgcatcca gtctaatttt ctccaatttt
      361 gcgcaactcc caggcagtat ggggaagcag atccggccag cgggccatga tcgtatccgc
      421 catggattca ggatcgagat tcagatagtt gaaaagaatc tcgactttgc cctgttcaat
      481 aaattcatcc gggattccca gtcgcagcac ctcggcctga atatgatcat ccgccagcag
      541 ttccgctacg gctgcgccaa aacctccctg caaacagttg tcctcaatgg tcaccagttt
      601 tcctgttttt actgccagcg ctttaatggc ttcgtcatcc agcggtttta taaaacgcgc
      661 attgagcaga ctggctgcaa tcccctgctg cgccagaatg gcaatcagtt cccgggccat
      721 ttccacggtc cgtccgatgg caataatgcc tacattgtca ccctcatgca gctttaccga
      781 acggccgatc ggcagcgtac agcgttccgg tttgatggcc acccccgctc caatcccgcg
      841 cgggtagcgg atggctgccg gtcccttgat ggtaaaagca gtatgcagca tatccatcaa
      901 ttcattctcg tctgccgggg ccataatggt catattggga atggagcgca gataggccag
      961 gtcaaatacc ccgtgatggg tcggcccgtc ctcgcctacc aagcccgccc ggtctacggc
     1021 aaagatgacc ggcaggttct gcagagcaac atcatggaca atctggtcat aagcccgctg
     1081 taaaaaggtt gaataaatgc tcaccaccgg tttcagcccc gctttggcca taccggccgc
     1141 catcgtcacg gcatgttgct cacagatgcc gacatcaaaa aagcgctccg gaaatttgcg
     1201 ggcaaagttc ttcaaaccgg taccgctggc catggctgcg gtaatggcaa ccaccttgtc
     1261 atcctggcgg cctttgtcaa ccataaactg tccaaacact tctgtataac tgggcaatgg
     1321 tttgcggatc gtttgaccgg tatcgatatc aaaaggtcct atgccgtgaa acatgtccgg
     1381 atctttaagg gccggctgat agccccggcc tttttgggtg attacatgca ccaacaccgg
     1441 gcctttcatt ttacgcacgt tgctgagcac tactgtcaat tcctcaagat tgtggccatt
     1501 gaccggtccg atataagtaa agcccagttc ttcaaaccag attccgggca ccatgatata
     1561 tttgacggta tccttgaatc gtcccgccgc tctggccaga ttgggcccga tgccgggaat
     1621 gcggcttaaa acgccttcaa tttcttcctt ggtacgggca taggaagggt ccgtccgcaa
     1681 acgattcaga taagatgaca tggcgcccac atttttggaa atggacattt cgttgtcatt
     1741 gagcacaacg gtgagatcac aaccggcatt gccggcgttg tttaatgcct cataggtcat
     1801 tcctccggtc aaggctccgt cgccggttac ggcaatgacc gaataatgtt ccttctgaat
     1861 atcgcgggcc agtgccatgc cgagggctgc cgagatggaa gtactgctgt gtccggtatt
     1921 gaatgcatca tgaggcgact cctcggcttt gggaaagccg ctgattcctc cgtattgacg
     1981 cagtgttgac aaacgagttt gtcgtccggt caggatttta tgcacataac tctgatgacc
     2041 aacatcccag ataattttat cctggggggt atcgtaaaca tagtgcagcg ccatggtgag
     2101 ttccacaacc cccaaattgg cagccaaatg accgccgctg cgggaaacgc tgtggatcaa
     2161 ctgctggcgg atttctgccg ccagttcatt taatgccgga atggacattt tttttatatc
     2221 caggggtgaa ttaatggttt taaaatcagg caatggctca ccttccacct ttaaaaaatt
     2281 cgatgccggt cagtttttcc caccaggcca tgatcaacgc tacccgaaag ataatggtgt
     2341 tggcattctc gatggcaatg ctcttgccaa ccgccttgcc gccgaccgtc agcgctgcaa
     2401 tcagactggt catgaccgcc ccgctcaaaa ccgtatccgg agcagagaaa ctgtccacca
     2461 gttgaaagac aattgccgca ccgatggcac cgctgagggt tccggcaata tcaccgatga
     2521 catcgttgca gtaattggca acaacgctgg catttttggt tattctgacc gcctgggaag
     2581 cgccggtgat ttttttggcg gcccgggcat tgaacggtgc cagcgttgct gcggccgcgg
     2641 ccgttccgat aatgtcaaag aagattccca aagcaataat cagcagcaag aaaatcatgg
     2701 cgatccaaac attgtgcatc gcattgacca gcgcttggga acccacactt accaaaagcg
     2761 caataaaaaa tgtaaaaaaa gcaacccata aggcggctat gagattgttg cgattgaatt
     2821 gatcacgcaa gcgattgatg ttcctccata aataacggaa aaggcaaaag gttatctgtt
     2881 gagtaaacgc tgtgacatag gtccagcagg ttttccccgc agttctcccg actgtcgcca
     2941 gacgggcggt ttccctttat gactgcttca tgccgtcgcc gtacatgggg ctggattacc
     3001 actaagtgca cactttaatc ctcaacaaat cgtgcattac acacacagtc taggagtttt
     3061 ccccaacaac ccacatcctt ttctagcctc ttttgcagtt gatgatttca aagcaggcaa
     3121 ccatccttcg gccaaagcat cattgcctcc aaaagctcat ccgccatcac cacgcaaggc
     3181 aggctacact gcacccagta ctaccagtat gcaggttttc ctgtgatcgg ttgcgatcac
     3241 aagcctccac ggatgatggg gtcgtatccg catgccattg cgggttgccc caacatcctt
     3301 aactcccagc gccgacccct gattgggcat cacaagccgg taccagaatc ttcatcgatg
     3361 tgcccgttac ggatttttag gcccgtattg aaaaaggata gggttgacta gaatactgca
     3421 accttttgcc ctgtcattcc agtaagacat tataccataa tcgcagacaa agaaaaagca
     3481 ctctcctgcc gcggcagtag cgggtttaac tggtccggtg cagcgaataa agggccagat
     3541 t
//
LOCUS       DAOTHZ010000143         3506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_31682_length_3506_cov_7.783541, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000143 DAOTHZ010000000
VERSION     DAOTHZ010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31682_length_3506_cov_7.783541"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="P5549_10375"
     CDS             <1..588
                     /locus_tag="P5549_10375"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRW13513.1"
                     /translation="STTFEPAMLAEYNPDLLVLAVGAKPIMPTIPGADHKIVSLESVL
                     SGECTIKGGNVVVIGGGSSGCEVAEYLADAGNTVTIIEQAPQLAIGLENITRLGLLAS
                     LKAKGIMTKTSAVVKEINGQEVIASRMTDGIEERIPADLVVAACGYVPESKLYQAVKD
                     QVGQVYVIGDAVTARGVEEALAEAVMTAYRLKTSC"
     gene            1009..1572
                     /locus_tag="P5549_10380"
     CDS             1009..1572
                     /locus_tag="P5549_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRW13514.1"
                     /translation="MAIKLSPATEEQKKLLAKVEKEAGTSVNLCYQCGKCTAGCPAAF
                     AMDYPPRQVIRLLQLGLIDQALRAESIWICASCETCSARCPRGVDIASLMDTMRRQAL
                     KQGKADKRVAAFNKAFLSNVKNFGKTFEAGLLLRYNLTTGQLFKDMELGLPMMIRGKV
                     GVLPERIKAREEMKKIFERAEKLGGDE"
     gene            1572..2819
                     /locus_tag="P5549_10385"
     CDS             1572..2819
                     /locus_tag="P5549_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRW13515.1"
                     /translation="MKFSYYPGCSLDGSGIEYGMSMERTARILGFELEELEDWNCCGA
                     TSGHNTDQLLSLALPARNLAIAEKTGMSMMTPCAACYSRHRHAEHVIQHDPEMKKKIE
                     QVLETELQGTSETVSILDILANKIGTEAIAAKVTNPLTTMKAACYYGCLLVRPVEHTG
                     FDDAEDPQTMDKVMKALGADAIEWSHKTECCGAAMVTSRPDVGNPMLYRVLKDAKESG
                     AECIVTACPLCMLNLDMRQARIEKQFKEKLDLPVYYVTELLAIACGDDPKTVGIEKHF
                     VEAGKYLQIVKVRAAEAKAAAEKAAAEAAAKAKEKAAAAKDKAAAGKTKAPEKAKSEG
                     DIAEPDPKKVEAFKTALTKNPAKMAAKLVADEAQAGILVSILDEKRIGKLADLMATDM
                     ELAKTAAAAFVTEELKRQGECQD"
     gene            2891..3472
                     /locus_tag="P5549_10390"
                     /pseudo
     CDS             2891..3472
                     /locus_tag="P5549_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT         1021 a          769 c          941 g          775 t
ORIGIN      
        1 agtactacct ttgaacccgc catgctggct gaatacaatc ctgatctgct ggtgttggcg
       61 gtgggggcga aaccaatcat gccaaccatt cccggggcag accataaaat cgtgtccctg
      121 gagagcgtat tatccggaga atgtacgatt aagggcggaa atgtcgttgt tatcggcggc
      181 ggcagcagtg gttgtgaagt ggcagaatac ctggctgatg ccggtaatac cgtgaccatt
      241 attgaacaag ccccgcaatt ggcaattggt ctggaaaaca tcacccgttt gggattgctg
      301 gccagcctca aagccaaagg catcatgacg aaaacatctg ctgttgtgaa agaaatcaac
      361 ggtcaggaag tgatcgcaag tcgcatgacc gacgggatcg aagaaagaat tccggcagat
      421 ctggtcgtgg cagcgtgtgg ttatgttccg gagtccaagc tttaccaagc agtaaaagac
      481 caggtagggc aagtatatgt cattggtgat gctgttaccg ccagaggcgt cgaagaggcc
      541 ttggccgagg cagtcatgac ggcctatcgc ctgaaaacca gctgttaatg gataaagcag
      601 ggacgaaagg tattatttta tagaaactta aagaaagacg gacggcaagc aaacaggcgt
      661 tgcaataaac gcctgtttat tgcaattgtg tataccgatt attgatttat actataaata
      721 taagcatcat ttatggtttt atatgcgatt aatgttgtca ttaacaaaat attaacattc
      781 acaatgctct aaaatataat ataatctatt aatattaatg aaaattcgaa aggctgtaaa
      841 atcgatatca atccgttcaa cttgcattta agacaggatt ctcttattat ttagtgtgaa
      901 taaacagata aatagcgaca aatatcgcga atattagtcg attatttaat agattattca
      961 tcatcatgaa tcggcgagga ttcaaggaaa ggagcgaaaa ccgtggatat ggcaataaaa
     1021 ttatctcctg ccactgagga acaaaaaaaa ttgctggcca aagtagagaa agaagcaggc
     1081 acaagtgtca acctttgcta tcaatgcggg aaatgcacag caggttgtcc ggcagcattt
     1141 gccatggatt atccccctag acaggtcatc cgattgctgc agttaggctt gatcgaccaa
     1201 gctttgcggg ctgaaagtat ttggatatgt gcctcctgtg aaacctgttc ggctcgttgt
     1261 ccgcgtggcg ttgatattgc ctcgttgatg gataccatgc gcagacaggc attgaaacag
     1321 ggaaaagcgg ataaacgggt agctgccttc aacaaggctt ttttatccaa cgtgaagaat
     1381 tttggcaaaa ctttcgaggc tggcttgttg ttgcgttaca atttgacaac cggacagctt
     1441 ttcaaggata tggaactggg acttcccatg atgattcgcg gcaaagtagg cgtattgcca
     1501 gaaagaatca aagctcgtga ggaaatgaaa aaaatctttg aacgcgcaga gaaactggga
     1561 ggggatgaat aatgaaattc tcatattacc cgggctgttc tctggatggc agcgggatcg
     1621 aatatggcat gtcaatggag cggacggcca ggatcctggg ttttgaactg gaggaactgg
     1681 aggattggaa ttgctgcgga gcgacttccg ggcacaatac cgaccaactc ctttccttgg
     1741 cgctgccggc cagaaacctg gcgattgcag aaaaaaccgg gatgagcatg atgactccct
     1801 gtgccgcttg ttacagccgc catcgtcatg ctgagcatgt cattcagcat gatccggaga
     1861 tgaaaaagaa aatcgaacag gttttagaaa cggaactgca gggaaccagt gaaacagttt
     1921 ctattttgga tatactggcc aacaagattg gaaccgaggc cattgccgcc aaggtgacca
     1981 atccgcttac caccatgaaa gcagcttgtt attatggatg tttgctggtc agacccgtcg
     2041 aacataccgg ttttgatgat gccgaagatc cccagaccat ggacaaggta atgaaagctt
     2101 tgggagcgga tgcgatcgaa tggtcacaca agaccgaatg ttgtggagcg gccatggtca
     2161 cttcgcgccc ggatgtaggc aatcccatgc tttacagagt gctgaaggat gccaaggaat
     2221 caggggcaga atgcatcgtc accgcctgtc cgctgtgcat gttaaatctg gatatgcggc
     2281 aggcgaggat tgaaaagcaa ttcaaagaaa agttagatct gccggtttat tatgtcactg
     2341 agctgctggc gattgcctgc ggagacgacc cgaaaacggt aggcattgaa aaacattttg
     2401 tggaagccgg caagtatctg caaatcgtta aggtgcgggc tgccgaagcc aaagcagcgg
     2461 ccgaaaaagc agctgcagaa gcagctgcca aagccaagga aaaagcagcc gctgctaaag
     2521 acaaggctgc tgccggcaaa accaaagctc cggaaaaagc caagtcggaa ggagacatcg
     2581 ccgagcctga tcccaagaaa gtcgaggcct tcaagacggc tttgaccaaa aatcccgcta
     2641 aaatggcagc caaactggtt gcagatgaag cccaggcagg aattttggtt tccatcctgg
     2701 atgagaagag aattggcaaa ctagctgact tgatggctac cgacatggaa ctggccaaga
     2761 ctgctgccgc cgcctttgtg acggaagagc tgaaacgtca gggtgaatgt caggattaaa
     2821 cccggcactt gcctgagtag ctgggatagt gataaacgat gtcagaaaac tgggaaggga
     2881 gacggagtag atgaagaaac gtgtaggcgt atttgtctgc gagtgtggta ccaacatcgc
     2941 tgccaccgta gacactgttc gggtggccga atacgcaaaa acattgcccg gggtggtcta
     3001 ttccaccaac tataaataca tgtgttcaga tccgggacag gaactcatcc gcaatgccat
     3061 ccaggaacaa caactggaac aggtagtggt tgcttcctgc tcgccgcgca tgcatgaaaa
     3121 aaccttccgc aaggccaccg aagccggcgg attgaatcaa tatatgatgg aaatggtcaa
     3181 tatccgtgag caaaactcct gggtgcacca tgacaaggcg gaagccacgg ccaaagcaga
     3241 acaactggtc aaaatggcgg tatccaaggc cttgcgcaac aaacctttgc agattgccac
     3301 ggtccccatt accaagaagg ccatggtcat tgggggtggc atcgccggca tgcaggccgc
     3361 gctggatatc gccgagggcg gccatgaagt cgttctggtg gaaagagagg cctccattgg
     3421 cggaaagatg acccaactgg acaaaacctt ccccaccatg gactgcgccg cctgaatagg
     3481 cacccccaag atggttgctg tggcgc
//
LOCUS       DAOTHZ010000144         3440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_32496_length_3440_cov_6.113442, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000144 DAOTHZ010000000
VERSION     DAOTHZ010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32496_length_3440_cov_6.113442"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="P5549_10395"
     CDS             <1..418
                     /locus_tag="P5549_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRW13516.1"
                     /translation="KMSPKLDRHIKEIQPAVIRCLQAYDWPGNVRELQNIVERILLVC
                     EEGTITIAHLPQEIVNAAIGHKPERWEVSNIPAPSSTLVMNDRRTRKLAALEQEKEMF
                     LQALNMNAGNVTKAAEALGISRNTFYRKMKLFEIDN"
     gene            749..1297
                     /locus_tag="P5549_10400"
     CDS             749..1297
                     /locus_tag="P5549_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13517.1"
                     /translation="MNAKQITIVNAKHSKKDYDIHALSAEQVQMFTCPRMTEEVYSLG
                     VKLQYMVGDLYLKYADSGDKVRKNAFMGLALKQLDKKTEIQRLANIDLNEKLNYFYNN
                     GGPIIEPPVNEKTAREINGFFNRIAENYLLKMEELISESADNHVALEVEVNQMIVRML
                     TDLANLYHENEIRMAFKELIFL"
     gene            1464..3128
                     /locus_tag="P5549_10405"
     CDS             1464..3128
                     /locus_tag="P5549_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRW13518.1"
                     /translation="MAESYSQAFAEDVEKWRHTWESRLDPESPKHFEYPAYPLKWWFN
                     QWAEKTPEKPYILLGDVVLPYGVCNDMSRRIANALLDLGVVKGDRVAIMAPNVPQYVL
                     ALQALFKTGMIEVPTNPLYTVPELAHQFADSGAETVIVMAMFANKAIEILKDPSSPVK
                     RVIYFQVPSGAVEVESGEGIYDLNQLLATASNAEPDIEVTAEDIARLQYTGGTTGVPK
                     GCVLTNAMIFSQAIRTSTISARGFTVVAKEDYRTLGAIPLNHVYGFNFNVSVNLYTGG
                     SMVLVPIPTPDNLLAAIVKHSPTIFAAVPAMIIGLINHPNIANADISSLKGMFCGSAP
                     LAVETLKEFEALTGGHIIEGYGLSETINIITANSFSHRKYGSCGLIWPDTDLVIVDIE
                     TGTKVMPRGEFGEIICRGPQVISEYWQKPEETAITIREGWLYTGDIGYLDEDGFVFIV
                     DRKKDIIICSGFNVYPRDVDEVLFAHPKVLEACTIGVPDAKRGETVKVYVVLKPGETM
                     TAEEVIAHCRESLAPYKVPTQVEFIDAIPRTNVGKPDKKVLQAKGE"
     gene            complement(3245..>3440)
                     /locus_tag="P5549_10410"
     CDS             complement(3245..>3440)
                     /locus_tag="P5549_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13519.1"
                     /translation="LGLTLLILCAAPLMVVALVFLAGYALLNCLFSLINIKPKSREIQ
                     SDTIEEERIQDWPALQLKHV"
BASE COUNT         1010 a          773 c          794 g          863 t
ORIGIN      
        1 aaaaatgtca cccaaactgg accggcatat caaggaaatt cagccggccg tcatcagatg
       61 tctgcaggcc tacgactggc ctggcaatgt acgggaattg cagaacattg tagagcggat
      121 tctgctggtt tgcgaagaag gtaccattac cattgcccac ctgccccagg aaatcgtcaa
      181 tgctgccatt gggcacaaac cggaaaggtg ggaggtcagc aacattcccg ctccctcgtc
      241 aaccctggtt atgaatgacc ggcgtacgag aaaactggcg gccctggaac aggaaaaaga
      301 aatgttcctg caagctttga acatgaatgc cggcaatgta accaaagcgg cggaagcgct
      361 gggcatttcg aggaatactt tttaccggaa aatgaagctt ttcgaaatcg acaattaagc
      421 ataggtataa atgcaacagg tttgtcactt gcgatgattg ttgctgcaca gttatgttac
      481 agcaatgtta cgatttgtca taaatattgc cgataaacca atttggaata cactgtgtaa
      541 tgaagagaaa cacctttata tcgggcgttt ctcttttttt ataaaaaact ccaaagttgg
      601 cacataaaat gcttattata ttatcagata actgcgcaat aaacatcgtc cactatacag
      661 gaaggagttg tcacctataa acaaacaaag aagttccata ttgcggatga tgccgttaat
      721 tataaaatac gaaaaggagt cggtcagcat gaatgccaaa caaattacca tcgtcaatgc
      781 gaagcacagc aagaaagact acgatatcca cgctctatcg gctgaacagg tgcagatgtt
      841 tacttgtccg cgcatgacgg aagaagttta ttcattgggt gtaaaattgc agtatatggt
      901 cggcgattta tacctcaaat atgcagactc gggtgataaa gtccgtaaaa acgcatttat
      961 gggattggct ttaaaacagt tggacaaaaa aacggaaata caaaggctcg ccaatatcga
     1021 cctgaacgaa aagctgaatt acttttataa caacggcgga ccaattatcg aaccccccgt
     1081 taatgaaaaa acagcccggg aaatcaatgg gttctttaat cgtattgctg aaaactattt
     1141 attgaaaatg gaagaactga taagtgaatc tgctgacaat cacgttgccc tggaagttga
     1201 agtcaatcaa atgatagttc gtatgctgac ggatttggcc aatttatatc atgaaaatga
     1261 gatccggatg gcatttaaag aattgatatt tttataaata ctgatagcta cacttgcatt
     1321 acatgaacac aacagggcta caatacgggc cctgtttttt ttatatgcaa taataatgtc
     1381 atgacattat tattgcatta gaatatctaa tcgaataatg tataatgaaa taagagtaat
     1441 aatatgacag gagagaatca attatggcag aatcttattc acaagccttt gccgaggatg
     1501 ttgaaaaatg gcggcataca tgggaatccc gtttggaccc ggaatcgccc aagcattttg
     1561 aatatcctgc ttatccactg aaatggtggt ttaaccaatg ggctgaaaag acgccggaaa
     1621 aaccgtacat tcttttgggt gatgtcgttt tgccttatgg tgtctgtaat gacatgagcc
     1681 gcaggattgc caatgcgttg ctggatttgg gcgttgtcaa gggagaccgg gtcgccatta
     1741 tggctcccaa tgtaccccaa tacgtactgg cgctgcaggc cctgttcaaa accggcatga
     1801 ttgaagtacc gaccaatccg ctctacaccg tgccggaact ggcccatcag tttgccgaca
     1861 gcggtgccga gaccgttatc gtgatggcca tgttcgccaa taaggccatt gagattctga
     1921 aagatcccag ttcaccggtg aaaagagtca tctatttcca ggttcccagc ggggcagtgg
     1981 aagttgagtc cggggaagga atttatgatt tgaaccagct gttggcaact gccagcaatg
     2041 ccgaaccgga cattgaagtg accgctgagg acatcgccag actgcagtat acgggaggga
     2101 ctaccggggt acccaaaggc tgtgtgctga ccaacgccat gatcttctcc caggcaatcc
     2161 gtacttcaac catcagtgcc agaggtttta cggtggttgc caaagaagac taccgcacct
     2221 tgggggcgat tcccctcaac cacgtgtatg gctttaactt caacgtaagt gtcaatctct
     2281 acaccggcgg cagcatggtg ctggtaccca ttccgacccc tgacaacctg ctggctgcca
     2341 tcgttaagca cagccccacc atatttgctg ctgttccggc catgattatc ggtttgatca
     2401 atcatcccaa tatcgccaat gctgatatca gctcgctgaa aggaatgttt tgcggctccg
     2461 cccccctggc agtagagacc ctcaaggaat ttgaagccct caccggcggg catatcattg
     2521 aaggctacgg cttgtcggaa accatcaaca ttattaccgc taattccttt tcccatcgca
     2581 aatacggcag ctgcggcctg atatggcccg ataccgatct ggtcatcgtt gatatcgaga
     2641 ccggaaccaa agtcatgccg aggggtgaat ttggtgaaat catttgccgc ggaccgcagg
     2701 tgatcagcga atactggcag aaacctgaag aaactgcgat caccattcgg gaaggatggc
     2761 tttatactgg agacatcggc tatcttgatg aagatggctt tgttttcatt gttgatcgca
     2821 agaaagatat tattatctgc agcggattca atgtttatcc acgcgatgtt gacgaagtac
     2881 tgtttgccca tcccaaagta ttggaagcct gcacgattgg cgtaccggat gccaaacgcg
     2941 gggaaacggt aaaagtctac gttgttctca agcccgggga aacgatgacc gcagaagaag
     3001 tcattgctca ttgccgcgag agtttggctc cttacaaagt gcctacgcaa gttgaattta
     3061 tcgatgccat tcccagaacc aatgtcggca aaccggataa aaaagtactg caagccaagg
     3121 gagaatagag tttcccattc cttccgcaaa gttgtcaggt tgactgcaaa atctaatttt
     3181 catactacaa agggcgactg ttttaacaac agccgccctt ttaaaacaca ttcactgtat
     3241 ggcgttatac atgttttaac tgaagagcag gccaatcctg tatacgttct tcctcaatcg
     3301 tgtcagattg tatctctctg cttttaggct tgatgtttat caaactgaac aaacaattta
     3361 acagcgcata accggccaga aataccaatg ccactaccat caggggtgct gcgcaaagaa
     3421 tcagcaaagt caagccgagt
//
LOCUS       DAOTHZ010000145         3439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_32508_length_3439_cov_7.411939, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000145 DAOTHZ010000000
VERSION     DAOTHZ010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32508_length_3439_cov_7.411939"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2134)
                     /gene="lon"
                     /locus_tag="P5549_10415"
     CDS             complement(<1..2134)
                     /gene="lon"
                     /locus_tag="P5549_10415"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRW13520.1"
                     /translation="MLGNEPSEYRELPMLPLRGLLVFPYTVIHLDVGRKKSINAIEEA
                     MLGSKEIFLATQKEAQNDDPTENDIYVVGTIAEIKQILKMPGGTMRVLVEGICRARVV
                     QYQDGDEYINVLLEEFKNETAHKNSELEALTRTLIYQFEQYVRMSKKIPPETVATVVT
                     IEEPGRLADVIASHLSLRINEKQSILEALDISARLNILCDILAREMEVLELERKINIR
                     VRKQMEKTQKEYYLREQMKAIQKELGEKDERAAEVEELRQKIKEAQLPPEAFEKVNKE
                     LERLERMPAMAAEGVVVRNYLDWVLSLPWSKETRDRLDLDQAEKILDEDHYGLEKPKE
                     RILEYLAIRKLAKKMKGPILCLVGPPGVGKTSLGKSVARSLGRNFVRMSLGGVRDEAE
                     IRGHRRTYIGSMPGQVLQSIKKSGSRNPVFLLDEIDKMTADFRGDPASALLEVLDPEQ
                     NNTFTDHYLEIPFDLSKVMFITTANSVYNIPRPLLDRMEVIELSSYTEEDKMPIAMDY
                     LIPKQIKEHGLKRKNIQFSEDAVRKIIREYTREAGVRNLERQIASICRKVAREVVKNK
                     DLSVKVEADSVKKYLGTEKFRYGRAEKDHQVGVTTGLAWTETGGDILSIEVALIKGKG
                     KLTLTGKLGDVMKESAQAGLTYVRSKAEELGISQEVLENYDIHIHVPEGAIPKDGPSA
                     GITMATSLASAMSGRKVRSDVAMTGEITL"
     gene            complement(2262..>3439)
                     /locus_tag="P5549_10420"
     CDS             complement(2262..>3439)
                     /locus_tag="P5549_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HRW13521.1"
                     /translation="IGSVHDPIYQGAGAMGTAGIPQPKPGACTKAHGGVLFIDEIGEL
                     HPTQINKLLKVLEDRKVMLESAYYTEQDKVIPPHIHDIFKNGLPADFRLIGATTRNPE
                     ELPPAIRSRCLEIFFKPLDKAAIVMIIKNAAQKINMAIEADALEEIAKYADNGREAVN
                     IVQLAAGIAQVEKQTAISLSVIKKVLNNGRFVPRLDIKPLEAPLIGVVNGLAVHGANV
                     GTLIEVEAATVPVNQGGGKWTVTGVIEEEQIGSGDKSYRRKSMAKAAVENVLTILKGQ
                     YGLRIKEHDIHINFPGGVPVDGPSAGAAMATAIYSALQQVPVDNLLAMTGEVSVHGRI
                     KPVGGVMSKVEAARNAGITRVLIPQDNMLDIFNDFPDLQVIPVSEIKEVFDQALLFH"
BASE COUNT          726 a          957 c          831 g          925 t
ORIGIN      
        1 gcaaggtaat ctccccggtc atggcaacat cgctgcgcac cttgcgcccc gacatagccg
       61 aagccagaga agttgccatg gtaatgccag ccgatggccc gtctttggga atggcacctt
      121 cgggaacgtg aatatgaata tcgtaattct ccagtacctc ctggctgatg cctagttcct
      181 cagctttgga ccttacatag gtcaaacctg cctgggcgga ttccttcata acatcgccca
      241 gcttgccggt cagggtcaat ttgcctttgc ctttgatcag cgccacttca atggacagaa
      301 tatccccgcc ggtctcggtc caggccagtc ccgtggtcac acccacctga tggtcctttt
      361 ccgcgcgacc gtagcggaat ttttccgttc ccagatactt tttgacactg tccgcttcaa
      421 cttttacact gaggtctttg ttcttgacta cttcccgggc gactttgcgg caaatggaag
      481 caatctgtct ttccagattt ctcactccag cctcgcgggt atattcccgg ataattttac
      541 ggacggcatc ctctgaaaat tggatatttt ttcttttcag accatgttcc ttgatctgct
      601 tgggaatcaa ataatccatg gcaatcggca ttttgtcttc ttccgtatag ctcgacaatt
      661 cgattacttc catgcggtcc agcaagggcc ggggaatatt gtaaaccgaa ttggcagtgg
      721 taataaacat aactttggaa agatcaaagg ggatctccag gtaatgatct gtaaacgtat
      781 tgttttgttc tggatccaag acttccagca gggcggaagc cggatctcca cggaaatctg
      841 cggtcatttt atcgatttca tccaacagaa aaactggatt ccgggatcct gactttttga
      901 tgctctgcag gacctgccca ggcatggaac cgatataggt acggcgatga ccgcgaatct
      961 cggcttcatc acgaacgccg cccaacgaca tacgtacaaa gtttcgaccc agtgagcgcg
     1021 ccacggactt gcccagcgaa gttttgccta caccgggcgg tccgaccaag cacaggattg
     1081 ggcctttcat ttttttggcc agcttgcgga tggccagata ttccagaatg cgttccttgg
     1141 gtttctccag accataatga tcttcatcca ggatcttctc tgcctgatcc aaatcgaggc
     1201 gatcgcgggt ttctttcgac caaggcaggg acaacaccca gtccagataa ttcctgacca
     1261 ccaccccttc agcagccatg gccggcatcc tttccagtcg ctccagttcc ttgttgactt
     1321 tttcaaaggc ttccgggggg agctgggctt ccttgatttt ctgccgcaac tcttccactt
     1381 cggcggcacg ttcgtctttt tcgcccagtt ctttctggat agctttcatt tgttcccgca
     1441 gatagtactc tttttgcgtc ttttccatct gtttgcgcac ccgaatgttg atcttgcgct
     1501 ccagttccag cacttccatc tcacgtgcca ggatgtcaca aaggatgttg agacgggcac
     1561 tgatatccag cgcttccagg atggattgct tttcgttgat gcgcagggac agatgggatg
     1621 cgataacatc cgccagccgg cctggctctt cgatggtcac caccgtcgcc acggtttcgg
     1681 gcggaatctt tttgctcatg cgcacgtact gttcaaattg atagatcagc gttctggtca
     1741 gcgcctccag ctccgaattc ttatgggctg tttcgttttt aaattcttcc agcaacacat
     1801 tgatatactc gtcaccgtct tggtattgaa ccactcgcgc ccggcagatc ccttccacca
     1861 gcacccgcat ggtgccgccg ggcatcttta aaatttgttt gatttcggca atcgtgccga
     1921 ctacatagat atcattctcg gtcggatcgt cattctgcgc ttccttttgg gtcgccagaa
     1981 agatttcctt gctgcccagc atggcttctt cgatcgcatt aatggatttt ttccggccga
     2041 catccagatg aattacggta tatggaaaaa ccaacagccc tctgagaggg agcattggca
     2101 gttcacggta ttcactgggt tcatttccca acataataac acctcccagg caaattcatt
     2161 ctactatatg tactgggcct gtgcaagaat gcctgccgca aggccaaaga aaaagaaccg
     2221 gacacgggta gcggccggtc ctgtcaacat gttctgttat attaatgaaa caacagtgcc
     2281 tggtcaaata cttctttgat ttcgctgact ggaatgactt gcagatccgg gaagtcgttg
     2341 aagatatcca gcatattgtc ctgcggaatc agaactcttg tgatgccagc attgcgggct
     2401 gcctcaacct tgctcatcac cccgcccacc ggtttgatgc ggccatggac ggagacttcg
     2461 ccggtcatgg ccagcagatt gtccaccggc acctgctgca gggctgaata gatggcggta
     2521 gccatggctg ctccggcaga cggtccatct actggcaccc cgccgggaaa attgatatga
     2581 atgtcatgct ctttgattct caagccgtat tgccccttca atatggtcag gacattttcc
     2641 accgcagctt tggccatgct tttacgccga tagcttttgt ctccggaacc gatctgttct
     2701 tcctcaatga ccccggtcac ggtccatttg ccgccaccct ggttaaccgg cacggtcgct
     2761 gcctcaactt caatcaaggt ccccacgttg gccccgtgga cggccaatcc gttgacgaca
     2821 ccaatcagcg gcgcttccag gggtttgata tccagacggg gaacaaaccg tccgttgttc
     2881 agaactttct taatgaccga caggctgatc gcggtttgct tttccacctg cgctatgccg
     2941 gcagccagct gtactatatt gaccgcctcg cgtccgttgt cggcgtattt ggcgatttcc
     3001 tccagggcat cggcttcgat tgccatgttg atcttttggg cagcgttctt gatgatcatt
     3061 acaatggcgg ctttatccag cggcttaaaa aatatctcta gacaacggga gcgaatggca
     3121 ggcggcaatt cctccggatt gcgcgtggtg gcgccgatga gccggaaatc agccggcaaa
     3181 ccgtttttaa aaatatcatg gatatggggc gggattacct tgtcctgctc cgtgtaatag
     3241 gcactttcca gcattacttt gcggtcttcc agtactttga ggagcttgtt gatctgggtg
     3301 ggatgaagct caccgatttc atcgataaac aagacgccgc cgtgggcttt ggtacaggcg
     3361 cccggtttgg gctggggaat gccggccgta cccatggccc cggccccctg ataaatcgga
     3421 tcatgcaccg agccaatca
//
LOCUS       DAOTHZ010000146         3406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_32924_length_3406_cov_5.406147, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000146 DAOTHZ010000000
VERSION     DAOTHZ010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32924_length_3406_cov_5.406147"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2174)
                     /locus_tag="P5549_10425"
     CDS             complement(<1..2174)
                     /locus_tag="P5549_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13522.1"
                     /translation="MKTLTRLFMVTALTLITFLCSGQTAEQAPDKPTPPTYGSLDYWQ
                     EKIQDGKTNYFELKSELSNYWNNREKIKGSGYKQVMRWLDAQAPFAMPDGSLRSPQED
                     VNVAMNFMQMNSNQAITGNWTCLGPNQPVYSTGTTNDIVAGLGRVTAIALHPTNDQIV
                     YVGAPVGGLWVSYNGGASWENLNTDNISALGVSAIAINPEHPNIMYIGTGDRDGHSSR
                     GVGIYKSIDGGLTWEAKPLDTRTDHTVSKIIIHPTYSNIILVGSNYGIFKSYDGGSTW
                     ELKLSKNIMDIETRPGTPEILYCVDRTAFYKSTDHGNSWALKYWTNAHRVAMSVTPAN
                     PDKVFLLCTRDSKFYKAIEVTQSGDHFVDLNASGFSAGEGQGGYNLDVIYDPLNENIM
                     YAGMVNFYKSTNGGVTWVNQEDVYADDQHIFDFHPVTHRLFIGNDSGIWYTDNGSDYV
                     YSSNGLIVGTSYRIDVSPQNPAHIVTGNQDAATFVTNGGYWYRSIGGDGITCRFDPTN
                     ENYVYGSSQNGHVARATGGGTGSSTFKTIAANGSHGINQNANFQTSFMVSYFNPNMMF
                     YGAHDVWRSNNIKAADPDNVQFSKISDNIAGTDEGTTIEFIEQSRAQESLLYIAYNNG
                     RMFRTSNALDNSPVWVELTQPNGWGGVRFESHPTNPNIVYLVRGRNVYRSSNRGNSWQ
                     DITGNLPELGMMSIAYMNNSPEGLYVGTTAGVYYKDTTMTDWV"
     gene            complement(2228..2509)
                     /locus_tag="P5549_10430"
     CDS             complement(2228..2509)
                     /locus_tag="P5549_10430"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRW13523.1"
                     /translation="MTTCSMITFHRATYDDGFGFSCMQAAYKLGIRGRMDYSPLQGVT
                     ILAEGEEESLSAFIDWINQHAGLINHFSNQQLNRQPFNYKEFDIYRHTA"
     gene            complement(2622..>3406)
                     /locus_tag="P5549_10435"
     CDS             complement(2622..>3406)
                     /locus_tag="P5549_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13524.1"
                     /translation="SRVKDSIFTNETNDLISRYEIMYETEKHEKENQLLLRDNEIKAR
                     RQKMLLIFSAGLLLLLIMAVSLFRVRSKYLNQQQQILQQDNYLAGLEIARKDTANQVL
                     EERIFAEQQINRLEREKHMAEIEVKNNELANSLLNIVRKNEIMYEIRERIRTSGNGDA
                     LNELVRYINSNTDTDHNWKKFRLEFEQVHAGFFDRLKQSYPDLTEHDARLAACLRINL
                     SSREIAGMMNVSLETAHKNRQRLRKKMNLPPSCDLMSELKKI"
BASE COUNT          788 a          808 c          809 g         1001 t
ORIGIN      
        1 acccagtctg tcatggttgt atccttataa tacactccgg ctgtagttcc cacatacaac
       61 ccttccggtg aattattcat ataggcgatg gacatcatgc caagttcagg caggttacct
      121 gtaatgtcct gccagctgtt gcccctgttg ctggaacggt aaacattcct tcccctgaca
      181 aggtatacaa tgttcgggtt ggtcgggtgc gattcgaacc tgacgccacc ccatccgttg
      241 ggctgagtca attctaccca gactggtgaa ttatcgagtg cgttgctcgt ccggaacatc
      301 cttccattgt tgtatgcgat gtaaagaaga ctttcctgcg ccctggattg ttctatgaat
      361 tcaatggtcg taccttcgtc agttccggct atattgtctg atatttttga aaactggaca
      421 ttatccgggt cagcagcttt gatgttgttg ctcctccaaa catcatgggc gccgtaaaac
      481 atcatgttag ggttaaaata gctaaccata aacgatgttt gaaagttggc attctggttg
      541 attccatgag agccattggc ggcaattgtc ttaaatgttg aacttccggt tcctccaccc
      601 gtagcacggg caacatgccc attctgacta cttccgtata catagttttc attggttgga
      661 tcaaacctgc atgtgatgcc atcaccaccg atggagcggt accagtagcc gccattggtc
      721 acaaaagtgg ccgcatcctg attcccggta acgatatggg cagggttctg aggagagacg
      781 tcgatgcggt agctggttcc cacaatcagg ccgttgcttg agtaaacata atcactcccg
      841 ttgtcggtat accagatgcc cgaatcattt cctatgaaaa gtcggtgtgt aacaggatga
      901 aaatcaaata tgtgctgatc gtccgcataa acatcttcct gattcaccca tgtcacccct
      961 ccgtttgtcg atttgtagaa attgaccatg ccagcataca taatgttttc gttcagcgga
     1021 tcatagatta catccagatt atatccgccc tgaccttctc cggcgctgaa gccactggca
     1081 ttcagatcaa caaaatgatc acccgattga gtcacctcta ttgccttata aaatttggaa
     1141 tcccgtgtgc aaagcagaaa taccttatca gggttggccg gcgtgacact catggcaacc
     1201 cggtgggcgt tggtccagta ctttagtgcc cagctattgc cgtggtctgt tgatttgtaa
     1261 aaggcggtgc ggtccacaca atagagaatt tctggcgtcc cggggcgggt ttctatgtcc
     1321 atgatatttt ttgacagctt taactcccag gttgatcctc cgtcgtatga cttgaaaata
     1381 ccataattac tgcccaccag gataatattg gaataggtgg gatgaatgat gatttttgaa
     1441 acagtatggt ccgttcgggt atcaaggggt tttgcttccc atgttaatcc accgtcaatt
     1501 gatttatata tacctactcc acgggaactg tgaccatccc ggtctccggt cccgatatac
     1561 atgatattcg gatgctcggg attgatggcg attgctgaaa cacccagtgc ggatatattg
     1621 tcggtgttca gattttccca gctggctccg ccgttgtatg agacccataa accaccaacg
     1681 ggagctccaa catatactat ctgatcgttg gtaggatgca aagctatggc ggtgaccctg
     1741 cctagcccgg ctacgatatc gtttgtagtg ccggtgctgt aaaccggctg gtttggcccg
     1801 aggcatgtcc agtttccggt aatggcctga ttactgttca tttgcatgaa attcatggcg
     1861 acatttacat cttcctgagg cgaccttagg ctgccatccg gcatggcaaa gggcgcttgt
     1921 gcatccagcc agcgcatcac ctgtttgtag ccggagccct ttattttttc cctgttgttc
     1981 cagtaattgc tcagttctga cttcaactca aagtaattgg ttttcccgtc ctgaatcttc
     2041 tcctgccagt aatccaggct gccatatgtg ggtggggttg gtttatctgg agcctgctct
     2101 gcggtttgtc cggagcataa aaatgtgatc agcgtcaggg ccgtcaccat aaaaaggcgg
     2161 gtcagtgttt tcatgatttc agtattttta ttaaagcaga attgtataaa tcaatgataa
     2221 tcaattacta agcggtgtgc cggtagatgt cgaattcctt gtagttgaaa ggctggcggt
     2281 ttaattgctg attgctgaag tggttgataa gtccggcatg ctgattgatc cagtcgataa
     2341 aagccgaaag gctctcttcc tctccttcgg ccagtatggt gaccccctga agggggctgt
     2401 aatccattct cccgcggata cccagtttat aggctgcctg catgcatgaa aaaccgaagc
     2461 cgtcatcata ggtggcccgg tgaaacgtaa tcatggagca ggtggtcata actgaacgtg
     2521 ctggttacac aaatttaagt cacctcaccg gggtccggca aattttcggg agggacaaag
     2581 agggggacaa gcaagatttt ttttgtagca ggaatatatg ttcagatctt cttcaactcg
     2641 gacatcaggt cgcaactggg aggcaggttc attttttttc gcagccgctg cctgttttta
     2701 tgcgcggttt caagggacac attcatcatg ccggcgattt ccctggagct caggttgatg
     2761 cgcagacagg cggccaggcg ggcgtcgtgc tcggtaagat caggatagga ttgcttcagc
     2821 cggtcgaaga aacccgcatg aacctgctca aattccagcc tgaacttttt ccagttgtga
     2881 tcggtatctg tatttgaatt gatataccgg acaagttcgt tcagggcatc accattgccc
     2941 gatgtccgga tccgttcccg gatttcgtac ataatttcat ttttcctgac aatattgagc
     3001 agcgagttgg ccagttcatt gttttttact tcaatttcag ccatgtgttt ctcccgttca
     3061 agcctgttga tctgctgttc ggcgaatatc ctctcctcca gcacctgatt cgcggtatcc
     3121 ttccgtgcaa tttcaagacc tgcaagataa ttgtcctgct gcaggatttg ctgttgttga
     3181 ttcaggtatt ttgaccttac cctgaaaaga ctcacggcca tgatcagcag cagcaatagc
     3241 cctgcagaaa aaatcagcag cattttctgc cttcttgctt taatctcatt gtcccttaaa
     3301 agcagttggt tttctttttc atgtttttca gtttcataca tgatttcata acgggagatg
     3361 aggtcattcg tttcgtttgt gaaaatggag tcttttaccc gggagt
//
LOCUS       DAOTHZ010000147         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_33444_length_3365_cov_7.125076, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000147 DAOTHZ010000000
VERSION     DAOTHZ010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33444_length_3365_cov_7.125076"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..243)
                     /gene="rpmF"
                     /locus_tag="P5549_10440"
     CDS             complement(73..243)
                     /gene="rpmF"
                     /locus_tag="P5549_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRW13525.1"
                     /translation="MGVPKRRQSHSRKNKRRSEWRKIDAPGLVECPQCHELKMPHHAC
                     LNCGYYKGKNVI"
     gene            complement(264..764)
                     /locus_tag="P5549_10445"
     CDS             complement(264..764)
                     /locus_tag="P5549_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRW13526.1"
                     /translation="MQINLARLKLRPQQSEDFYLEAQGNNDFFQGSGAIFHDRIIVDL
                     QVENTGRMFLARGKVKTLLVLPCSRCLQDTCIAIDTDVELTLVPAGQAGKPEPEEDVL
                     VFQGDLVDLRVPVHEAIFMAIPMIPLCHSDCRGLCPVCGKDLNQGPCSCQQKEIDPRW
                     EKLKNL"
     gene            947..2287
                     /locus_tag="P5549_10450"
     CDS             947..2287
                     /locus_tag="P5549_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRW13527.1"
                     /translation="MKKMNFDSFIMRNGLVALSAADLADSPKIALALPKLLDFISTLE
                     LSADDFYVNLRNPEKPFSYYRGAVFLEFASNRNDLANLKKRIIKAENQLNQAVANRDF
                     ESFLRIVDPRLAPDLFMEVFSFIPDVDKYPLLQSMLAGNPYSREAFDPAFVSKASRYK
                     DAQKGMPLADEQGYVKIFASADHIGQLETQSLWYTDINRAIQQMISLAKREAEIFQAR
                     VHISAVNSDTPSQSEKEVSIAPGQTCQIKHLEMIKISGLLPLMQSQKLIGAYYTYARQ
                     IKAEWFHNPEGIHAVSHTRRVLLLGILLAHLEKLTEQERDLICLAAIYHDIGRLTDGY
                     DTTHGLASFRKLMSRGLLKPLPAEDMETVRFMIELHAVADQAAMKQFNKYHLQDTDRT
                     LKLYSLFKDADGLDRVRINDLNPDYLRNLGAHKLLLLAHQLYHEPNFEKRWLQS"
     gene            complement(2302..3150)
                     /gene="fdhD"
                     /locus_tag="P5549_10455"
     CDS             complement(2302..3150)
                     /gene="fdhD"
                     /locus_tag="P5549_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRW13528.1"
                     /translation="MPGRTARFSSDLRLLVEKECMIIELQPLYKSRRLHVYQQGKLIE
                     KNDPVVIETQLHLIVNDEPAAELVCSPQAFAELAVGYLLSENLLKDYPDLCSWRLDAQ
                     QQAIHLYTKHSWLGQAQHTRQINSYAGRGLGLQTETTGIAAVTSAKDLFTPAQIFAMI
                     DALENSGGTFHLTGGTHNAGLGYQGQLLVNFEDIGRHNAVDKVFGHAFLQQIPLNDKC
                     LVLSGRIASEILLKAARRNIALVVSRSAATGLAIDLAEALGITLVGFTRGDRFNIYCH
                     RERIQM"
     gene            complement(3211..>3365)
                     /locus_tag="P5549_10460"
     CDS             complement(3211..>3365)
                     /locus_tag="P5549_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRW13529.1"
                     /translation="YLGVKMDNEKNKVRGKEVDVATADSKVRIFVIPTNEELVIARDT
                     LRLSKK"
BASE COUNT          876 a          842 c          735 g          912 t
ORIGIN      
        1 ataaaaaaca aaaagtaaga tacagcctgg aagactatcg cttacttttt ggttctttta
       61 ctgggattgg tcttaaataa cgtttttgcc cttatagtaa ccacaattca aacatgcatg
      121 atgcggcatt ttcagttcgt gacactgggg acactcaact aatccgggtg cgtctatctt
      181 tctccattca gaacgacgtt tattcttccg tgaatgtgac tgtctccttt tgggaacgcc
      241 cattcatact acctccttcc caactataaa ttctttagtt tttcccatcg ggggtcgatt
      301 tccttctgtt gacaggaaca tggcccctga ttcaggtctt tcccgcaaac tgggcatagc
      361 ccccgacaat cgctgtgaca cagaggaatc atcgggattg ccataaatat agcctcatga
      421 acaggaacac gcaaatccac cagatctccc tggaaaacca atacatcctc ttcgggctct
      481 ggctttccgg cctgcccggc aggcaccaat gtcaattcga catcagtatc aatggctata
      541 caggtatcct gcagacaacg tgaacacggc aggacaagca aggttttcac ttttccccgg
      601 gccaaaaaca tacgcccggt gttctccacc tgcagatcaa cgatgatgcg atcatggaaa
      661 attgcgcctg atccttggaa aaaatcatta tttccctgtg cttccaggta aaagtcttcg
      721 ctttgctgcg gcctgagttt cagccgggca aggtttatct gcatacattc acctcataaa
      781 aacaccagat tcattataga ctgctcttct tgagcttgtc aataaacgac ttgggcatct
      841 gatgattgct tgctgatgat tttgtcttaa tgattccggc aggataaaca tagaataaaa
      901 acgaataaaa ccatgggaat ccaaatctat tctgtgaggt ttgccgatga aaaaaatgaa
      961 ttttgacagt tttatcatgc gcaacgggct cgttgcattg tctgcggctg atttggccga
     1021 ctctccgaag atagccctag ctttgcccaa attattggat tttataagca cgctcgaatt
     1081 gagtgcagat gatttttatg tcaaccttcg caatccggaa aaaccattca gctattatcg
     1141 aggtgcggtt tttcttgagt ttgccagtaa ccggaatgat ctcgcaaacc tgaaaaaacg
     1201 tatcataaag gccgaaaacc aactcaatca agcagtcgcc aaccgtgact tcgaaagctt
     1261 tctacgcatt gttgatcccc gtctggctcc ggatttgttt atggaagtat tcagttttat
     1321 tcctgatgtg gataagtacc ctttgttaca atccatgctc gccggcaatc cctacagtcg
     1381 ggaggcattt gatcctgcct ttgtcagcaa ggcgtcccgc tacaaggatg cgcaaaaagg
     1441 catgccgttg gccgatgagc aaggctatgt taaaatcttt gccagcgcag accacatcgg
     1501 ccagctggaa acccagtctc tttggtatac agacattaat cgcgccattc agcaaatgat
     1561 atcgcttgcc aagcgggaag ccgagatatt tcaagcccgg gttcatatca gtgctgtaaa
     1621 cagcgatacc cccagtcaat ctgaaaaaga agtctctatt gcgccggggc agacatgcca
     1681 gataaaacac ttggaaatga tcaaaatttc cggcctgttg cccttgatgc agagccagaa
     1741 gctgatcggc gcttattata cttatgcccg gcaaataaaa gcggagtggt tccacaatcc
     1801 tgaaggaatc catgctgtca gccataccag acgggtgttg ctgttgggaa ttttgctggc
     1861 tcatctggaa aaactgaccg agcaggaacg tgacctcatt tgcctcgcgg ctatctatca
     1921 tgatattggc cgattgaccg acggttatga caccacccat ggtctggcca gttttcgcaa
     1981 actaatgtcc cggggactgc tgaaaccact gcccgctgaa gatatggaaa cggttcgatt
     2041 tatgattgag ctgcatgcgg tcgcagacca ggctgcaatg aagcaattca acaagtatca
     2101 cttgcaagac actgaccgta cgctcaagct ctatagcctt ttcaaggatg ctgacggtct
     2161 tgaccgggtt cgtattaatg atctcaaccc ggattatcta cgcaaccttg gcgctcacaa
     2221 attgttgctg ctggcccatc agttgtatca cgaaccgaat tttgaaaagc gctggttaca
     2281 gtcttagccc atattcgcag attacatctg gatcctttct cgatgacaat aaatgttaaa
     2341 tctatcaccg cgggtaaagc ctaccaaggt aatccccaat gcttcggcca gatcgatcgc
     2401 cagtccggta gcggctgaac gggatactac cagagcgatg ttccggcggg ctgcttttaa
     2461 caggatttcc gaagcaatcc gcccgctcag caccaggcac ttgtcgttga gcggaatctg
     2521 ctggagaaaa gcatggccaa acaccttgtc aacagcgtta tggcgaccaa tatcctcaaa
     2581 attgaccagc agttggcctt ggtatcccaa cccagcgtta tgggttccgc cagtgagatg
     2641 aaaagttccg ccgctgtttt ccaacgcatc gatcatggca aatatctgcg ctggcgtaaa
     2701 taaatctttt gcgcttgtaa cagcagcaat gccggttgtt tcggtttgca gtcccagccc
     2761 tctgcctgcg tagctgttaa tctggcgagt atgctgcgct tgccctaacc aagagtgctt
     2821 cgtatacaag tgaatcgcct gctgctgtgc atccaaacgc caggaacata aatccggata
     2881 atcctttaaa agattctcgc ttaacaaata cccgactgcc aattctgcaa aagcctgtgg
     2941 tgaacaaacc agttctgccg ccggttcatc attgacgatc agatgcagtt gtgtctcaat
     3001 gacaaccgga tcgttttttt cgattaactt gccttgttga tagacatgta atcgtctgct
     3061 tttatataaa ggctgaagct cgatgatcat gcactccttt tccaccagca aacgtaggtc
     3121 actggaaaaa cgggcagtcc gcccgggcat actactgcaa aaagcgggaa tgctcccgct
     3181 tttcaacagt ataattcatg caattttgtt ttatttcttg gaaagtctca atgtgtctct
     3241 ggcgataacc agttcttcat tggtcgggat gacgaagatt ctgactttgg aatcggcagt
     3301 cgctacgtct acttccttgc ctcttacctt gtttttttca ttgtccatct ttacgcccag
     3361 ataat
//
LOCUS       DAOTHZ010000148         3300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_34235_length_3300_cov_8.461017, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000148 DAOTHZ010000000
VERSION     DAOTHZ010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34235_length_3300_cov_8.461017"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="P5549_10465"
     CDS             <1..783
                     /locus_tag="P5549_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRW13530.1"
                     /translation="QEAKVIITFGAVSSNHARQTAAAAALLGLECQLILTGKEPQLIQ
                     GNYLLDKILGAKTYCVRNREVEDTLARLTADNLKQGKRSYVIPAGGHTILGTMAYIQA
                     YQEMKQQFPHQLAGIIAAFGTGTTYAGLYLGSQLDANGIPVIGISVGGDQDWCEAEAG
                     QVIQQTLQHLDLPAPPLAPMEIHDEYVGEGYTIPYPRVRAAIELLARTEGILLDPVYS
                     GKAMAGLLDLVEKGRFQAGDNVMFLHTGGAPELFTMQHFMSE"
     gene            852..941
                     /locus_tag="P5549_10470"
                     /pseudo
     CDS             852..941
                     /locus_tag="P5549_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            1746..2144
                     /locus_tag="P5549_10475"
     CDS             1746..2144
                     /locus_tag="P5549_10475"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRW13531.1"
                     /translation="MLALTENYVLTCAGGLDELYQWEALSGEDIKPPREWQLVNSNLH
                     RQPPPLELAAIWFTVLQQQETERWLRTCHPELEEKTPLEIIQEEKGRERINRLLDKFA
                     SQLEENEEASRLVACMRARVEALSSESFLL"
     gene            complement(2258..2860)
                     /gene="yihA"
                     /locus_tag="P5549_10480"
     CDS             complement(2258..2860)
                     /gene="yihA"
                     /locus_tag="P5549_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRW13532.1"
                     /translation="MLVIKKAEFVSSFVDIKSLPEDNLPEIALAGRSNVGKSSLINKI
                     VNRKNLAKSSSTPGKTRTLNYYLINSAFYMVDLPGYGYARVSKQERDKWGKLIEKYLS
                     SRPQLRGVIQLLDIRHPPTENDVAMQQYLEHYQVPILLIATKTDKVSRNEKNKNLAVI
                     KKTMQLESDQLLITFSAETGEGVDEVKTALEEILTPPESE"
     gene            complement(3034..>3300)
                     /locus_tag="P5549_10485"
     CDS             complement(3034..>3300)
                     /locus_tag="P5549_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRW13533.1"
                     /translation="MSGRKVRSDVAMTGEITLRGRVLPIGGLKEKSLAAHRAGIYTIV
                     LPAENEKDLEDVPAQIKNVMNFVLVSHMDEVLAQALLPQAKGKR"
BASE COUNT          773 a          873 c          886 g          768 t
ORIGIN      
        1 caggaggcca aggtcatcat cacttttggt gccgtttcct ccaatcacgc tcgacaaacg
       61 gcagctgccg cagcgctctt gggactggaa tgtcaattga ttctgaccgg caaagaaccg
      121 cagctaatcc aaggcaatta cctgctggat aaaattttgg gtgccaaaac gtattgtgtc
      181 cgcaaccgcg aggtggaaga cactctggcc cgtttgacgg ctgacaatct gaaacaaggc
      241 aagcgctcct atgtcattcc ggctggaggc cataccatcc tgggtacgat ggcctacata
      301 caggcctatc aggaaatgaa acagcaattc cctcatcaac tcgccgggat cattgccgcc
      361 tttggcaccg gcaccaccta tgccggtctc tatctgggca gtcagctgga tgccaacggc
      421 attcccgtaa tcggaatttc ggtgggcggc gaccaggact ggtgcgaagc agaagccggc
      481 caggtgatcc aacaaacctt gcagcatctc gatctccccg ctccgccgtt ggcacccatg
      541 gaaattcatg acgaatatgt gggggagggc tataccatcc cctatccccg ggttcgtgcg
      601 gccatcgaac tgctggcccg caccgagggc atcctgctgg atcccgtcta cagtggcaaa
      661 gctatggcgg gcctgcttga tctggttgaa aaaggacgct ttcaggccgg tgacaacgtc
      721 atgttcctgc ataccggcgg cgcaccggag ctgtttacca tgcagcattt catgagcgaa
      781 tagtattgga cgtttgcaca atcgagttgg cgtcccggcg ttaaatatcc tgcagataaa
      841 aaagctattt atggaggaag gaaatcatgg gcaaaaataa aatcggccgc aatgacccct
      901 gtccctgcgg cagcggcaag aaatacaaac aatgctgtat ggtgctcgaa gacggtccga
      961 ccgccgacct gttcacgcgc tgcagccaga tgatggccgc tgtcaaactg aaactggatg
     1021 atgcttacaa agccaatatt aaacgcattc gcaaagatgc ccgtcagtat ttttgccatt
     1081 ataccgtcaa tcaacagctg accggggagc aggaagccct cttttccgac tggttgtggt
     1141 ttgattaccg tcaggaaaac cagcggacac tggcggagga ttacctcagc gatcatcgcc
     1201 agtatatgcc cgcgcagttg caagaatgcc tgcacgcctt gtccgactct tatttatcgg
     1261 tttatgaacc cacccatatc ggcgaccatt atctggaatt gcgggacatc ttcaccgaag
     1321 cccggcagca ggtcaccttg aaagaatcgc tggacgcgga tttgaatgaa aaacctttgc
     1381 tattgctggg aagaatggtg ggattcgcgg aaggtcaggt tttttccggc atggtcctgg
     1441 ccgttgacaa caatgacggt caagcctcct atctgcaaaa gcacgtccgt tatctggcag
     1501 ccatcaaagg cgaagctgac accgtcacgc tgttaaaaaa ccatgccgat gtcctgctgg
     1561 ggttgtttga ccatgcgttg cgaaaaaagc tgctcaacat caatgacatt cgctatctgc
     1621 cctttgctga cccgtcacag cacctcgcca ttcaagcgtg gctggaagcc aaccaaggga
     1681 tgcaatacgt caataaatat gaggatctga cctggtatca ggaccagcag gcgggggtat
     1741 ataaaatgct ggctctgacg gagaattatg tcctcacttg tgccggcgga ctggatgagt
     1801 tgtatcaatg ggaggcattg tcgggcgaag acatcaagcc cccccgggaa tggcaactgg
     1861 tcaacagcaa cctgcaccgt cagccgccgc cgctggagct ggccgccatc tggtttaccg
     1921 tcctgcagca acaggaaaca gaacggtggc tgcgcacctg tcatcccgag ctggaggaaa
     1981 agactccgct ggaaatcatc caggaagaaa aaggcaggga gagaatcaat cgtctgctgg
     2041 acaagtttgc ctcccagctg gaagaaaacg aggaagcctc gcggctggtt gcctgcatgc
     2101 gtgcgcgggt ggaggcattg agttcggagt cgtttttact ctgattaacg atagaaaagt
     2161 tcacccaacc gggctgtcct tggcggcgcg cagtgagatg cttcactggc gcctggcagg
     2221 acactttgca aggtccgggc agtgcgatcc gcattggcta ttcggattcc ggcggtgtca
     2281 gtatctcttc cagggcggtc ttgacttcgt caacaccttc gccggtttca gcagaaaacg
     2341 tgatgaggag ttgatccgac tccagctgca tggttttttt gatcaccgcc aggtttttgt
     2401 ttttttcgtt gcgggatact ttatcggtct tggtggcgat gagcagtatg gggacctgat
     2461 aatgctccag atactgttgc atcgcgacgt cgttttctgt ggggggatgc cggatgtcca
     2521 gcagttggat gacgccgcgc agctgcggac ggctgctcaa atacttttca atcaatttgc
     2581 cccatttgtc ccgttcctgt ttggataccc gggcatagcc gtagccgggc agatcaacca
     2641 tgtaaaaggc ggaattgatt aagtaataat tcagtgtgcg ggtcttgcca ggcgtggagc
     2701 tcgatttggc aagatttttt cgattcacga ttttgttgat gagcgaagat ttgcccacat
     2761 tggaccggcc tgccagagca atctcgggta gattgtcctc gggcagactc ttgatatcca
     2821 caaatgagct gacgaattca gctttcttga ttaccagcat caaatcatcc tcttcctggt
     2881 ctgaataaag cccaataacg tagtccgggc accggatgcg gcttgcttgg gcgcaaacag
     2941 ggggacacac cttttgtaag gaagtgcccc cgcaactgat tcatcaaatg aaggctcaac
     3001 caggctttat catacgactt tcctgtcaag gggtcaccgc ttgccttttg cctgggggag
     3061 cagggcttgg gccagtactt catccatatg gctgaccagc acaaaattca ttacattttt
     3121 tatctgggcc ggcacatctt ccaagtcttt ttcattttca gccggcaaaa caatggtata
     3181 gatcccggca cgatgggcgg ccagggattt ttcctttaat ccgccgatgg gcaatacccg
     3241 gccgcgcaag gtaatctccc cggtcatggc aacatcgctg cgcaccttgc gccccgacat
//
LOCUS       DAOTHZ010000149         3281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_34500_length_3281_cov_4.945133, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000149 DAOTHZ010000000
VERSION     DAOTHZ010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34500_length_3281_cov_4.945133"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1129)
                     /locus_tag="P5549_10490"
     CDS             complement(248..1129)
                     /locus_tag="P5549_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HRW13534.1"
                     /translation="MKKAYFKWFLDLLMLVFLITFVKAKMISLSYHEIGGLIIGAVFV
                     IHIVLNWQWVKNIAKVLFSSKLKARNKLMFWFNVLIFISMLTIIITGLFISEILFPGL
                     RVQGFAMRPLHEGMSYLALTLVGLHIGLNGRWLISVFKKMFSIIQIRPGINYVLRAVA
                     AAILIFGIFSAVQTDFYGKVPAMVSTYAAIYSGDLNGAGPIPYGLGEGLHKRGGLPPG
                     KFKGEGRGGEGQQQPLPGGLHRGQPGSGKGDFRGRHGQEGQPNQLFYMIITYLSIIGS
                     IAIVTYYADKILARKKG"
     gene            1302..1793
                     /locus_tag="P5549_10495"
     CDS             1302..1793
                     /locus_tag="P5549_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRW13535.1"
                     /translation="MKLSEFYEKVLAALLVICILGAGLFYLQIRDQEPVMPVQGKVGE
                     PRSDDVWMISRMVAAESRGEPFNGQVAVASVIVNRVRNPKFPGTVNGVIYQPLAFTPI
                     VDGQFWSVNPTDTHLRAARYALNGWDPSYGSIFYWNPATATSKWIWSRKIITRIGKHV
                     FGI"
     gene            1818..3188
                     /gene="ypeB"
                     /locus_tag="P5549_10500"
     CDS             1818..3188
                     /gene="ypeB"
                     /locus_tag="P5549_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="germination protein YpeB"
                     /protein_id="HRW13536.1"
                     /translation="MRNHKVLIGLLLVVGLAVAAGSYYSAQYYKQANASQNALEASYQ
                     NNFFNLIENMENLDVLLGKSLASNSAGQNAITFASVWSMAEMAKANLSNLPLGTINTV
                     RSNQYLSQLGDFSYSLAQKAADGQEINDSDWQKIRQLHNENRTIHKELRSLVELMQEK
                     QVRFGSLARVDKDKLPQESLAVLEGFGKLDERLQNEVPTLTYDGPFSDHVVNRSPRGL
                     TGAVINKTQAENIALQFIKKMNRTGRYTVGTTSLASGRIASYSVTLKPQEAGRSELIL
                     GISRKGGHVVLMMDGGEPAAAKRIGYAQAIRKAEDFVDKLDLGAFVATGHLAEGNELL
                     VNFAALQDGVVIYPDMIQVSVSLDTRNVAGYDASKYLLSHTNRELPAPALTEDEARAK
                     VNPRLNIERVRLALIPLGNLEERLTYEVKGKIDSDTYYVYINALTGSQEKILLIVETP
                     EGSRSI"
BASE COUNT          881 a          812 c          801 g          787 t
ORIGIN      
        1 ccaccatcta caaactgctg gccaaagacg tccaaaacag ggatagatta gtgaggagtg
       61 aggagtgagg agaacgcgta aagcgatctg ccccttgtca cctgaagcaa tagtaaaggg
      121 cgcacaatgc gcgccccttc tgttgtttgg ccatggattt gctatcgctc agccttcgcc
      181 tggttccaac tttcaacttt ctgcttttca attctctcca aactcctcac gcctcacccc
      241 tcactcctca ccctttcttc ctcgccaaaa ttttatcagc gtaatacgtt acaatggcaa
      301 ttgatccgat aatggacagg taagtgatga tcatgtaaaa caactgattg ggctgtcctt
      361 cctgcccgtg ccggcctctg aagtctccct tgcccgatcc tggctgaccg cggtggagcc
      421 ctcccggtaa aggttgctgc tgtccctcgc ctccccggcc ttctccctta aatttgccgg
      481 gcggcagtcc tcctcttttg tgcaaacctt cccctaaccc ataaggaatc ggtcctgcac
      541 cgttcagatc tccactgtaa atagcagcat aagtgctgac catcgccggt acttttccgt
      601 aaaaatcagt ctgcaccgcg ctgaagattc cgaaaatgag tatggctgcc gccacagccc
      661 tcagaacata atttatccca gggcggatct gtattatgga aaacattttc ttaaagacac
      721 tgatcagcca tctcccattc aggccgatat gaagccccac cagcgtcagg gccagataag
      781 acatcccttc atgcagcggt ctcatggcaa aaccttggac gcgaaggccg gggaacagga
      841 tctcggagat aaacagcccg gttatgatga tggtcagcat actgataaag atcagcacat
      901 tgaaccagaa catcagcttg tttcgggctt taagtttcga tgagaaaagt actttggcaa
      961 tgttcttgac ccactgccag tttaaaacga tgtggatcac aaacacagcc ccgatgatca
     1021 gccctccgat ctcgtggtag cttaaagaaa tcattttggc ttttacaaaa gtaattagaa
     1081 agaccaacat cagcaggtct agaaaccatt taaaataagc ttttttcaca gggctccccc
     1141 ctctatattc attatactat caatagaata agccagaact atgaacatct catgaacaaa
     1201 ctatgaagaa tcagtgataa aaacctataa gtgatgcttt tccttttgca taaggcgtta
     1261 aagtttttgg aaatactaga tagagcaaag gagggaagtt ggtgaagctc tcagaatttt
     1321 acgaaaaagt tttagcagct cttttggtca tatgtatcct gggcgcagga ttattctatc
     1381 tccagatcag ggatcaggag ccggtcatgc ctgtacaggg caaagtggga gaaccccgtt
     1441 cagatgacgt ttggatgatt tcccgtatgg tagcggccga gtcccgtggt gaacctttta
     1501 atgggcaggt agcggtggca tctgttatcg tgaaccgggt tcgcaatccg aaattccccg
     1561 ggacagtaaa cggcgtaata tatcagcctc tggcttttac gccaattgtg gacggacaat
     1621 tctggtcggt aaaccccacg gatacacatc tgcgcgccgc ccgctacgcc ctaaacggct
     1681 gggatccttc ttatggttcg attttttact ggaatccggc tactgcaacc agcaagtgga
     1741 tctggagcag aaaaatcatt acccggatcg gcaaacatgt ttttggtatt tgattttaga
     1801 aagaggtgaa cgaaagattg cgcaatcata aagtcctgat cggcttgctg ctggtagtgg
     1861 ggctggcggt tgccgccggt tcctattatt ccgctcagta ttataagcaa gccaatgcct
     1921 ctcagaatgc attggaagcc agttaccaga ataacttctt taatctgatc gaaaacatgg
     1981 aaaacctgga cgtgctgctg gggaaatccc tggcttccaa ctccgcaggg cagaacgcga
     2041 tcacctttgc cagcgtctgg tcaatggccg aaatggccaa ggccaattta agcaatctgc
     2101 cgttaggcac tatcaatacc gtgcgcagca atcaatacct gtcgcagttg ggtgatttca
     2161 gctattcatt ggcacaaaag gccgccgatg gccaggaaat aaacgatagt gactggcaga
     2221 agatccggca gctgcataat gaaaaccgga cgatccataa ggaattacgt tccctggtgg
     2281 aattgatgca ggaaaaacaa gtgcgcttcg gcagtctggc cagggttgac aaagacaagc
     2341 tgcctcagga aagcctggct gttttggagg gtttcggcaa actggacgag cggctgcaga
     2401 atgaagtacc taccttaact tatgacggac cattttccga tcatgtggtt aacagatccc
     2461 cacgcggact gacaggagca gtgatcaata aaacccaggc agaaaacatt gccctgcagt
     2521 ttattaaaaa aatgaaccgt accggccgat atacagttgg caccaccagc cttgccagcg
     2581 gtcgtattgc ttcctattca gtcaccctga aacctcagga ggccggaagg tctgaactta
     2641 tactgggcat tagccgcaaa ggcggtcatg ttgtcttgat gatggacggc ggggaaccgg
     2701 cggcggccaa acggatcggc tatgcacagg ccattcgcaa ggcggaggat tttgtggaca
     2761 aattagacct gggagccttt gtggccactg gtcatttggc ggaagggaac gaactgctgg
     2821 tcaactttgc cgcactgcag gacggtgtgg ttatctatcc ggatatgatc caggtcagtg
     2881 tttctttaga tacccgcaat gtagccggct atgatgccag caaatatttg ctgtcccata
     2941 cgaacagaga actgccggca cctgcgctca ccgaagatga agccagagcc aaagtaaatc
     3001 cccggctaaa tatagaaaga gtccgcctgg cccttatccc tctgggcaac ctggaagaac
     3061 ggctgaccta cgaagtgaaa gggaagatcg acagcgatac ttactatgtc tatatcaacg
     3121 ctttgaccgg ctcccaggaa aagatcctgc tgatcgtgga aacaccagag gggtcacgga
     3181 gtatataagt agaaagttga aaagtggaaa gtcggaacca ggcgctggcc aagcagtggt
     3241 caagtaattt ctgagcaata gccgtaggag ctttcttgat t
//
LOCUS       DAOTHZ010000150         3183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_35938_length_3183_cov_6.403133, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000150 DAOTHZ010000000
VERSION     DAOTHZ010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35938_length_3183_cov_6.403133"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="P5549_10505"
     CDS             <1..241
                     /locus_tag="P5549_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13537.1"
                     /translation="TRHNLQQELLRIKDKLATTVLFVTHDIDEALVLGDRILIMTRNG
                     QVRSIFANPLAKPRQPRMEGFAGMWGKIYDQLGES"
     gene            275..2251
                     /locus_tag="P5549_10510"
     CDS             275..2251
                     /locus_tag="P5549_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13538.1"
                     /translation="MTAAPLKRYLLAVLAVVNLLVLFYFGLGAFDLLKLSATVAVGTL
                     IPGLLLLHWWKLYPDTQYRFVLALVIGMSLDILLFIGASWLKLHFLIYLLPGIGLMLY
                     LQNRLYKKDGRSIKNAFQAFSVWHLTVLTAISLLVVGLIVWFFYIPNQLPGSQAVVYH
                     VDDPWHLGNIAEILHHWYPQDPRLAGMEFNYHIFYYVYMAFLAFISKVSLPVIYFRFY
                     IILFLYTLMGGAYFVASRWFHDRRAGILHLVIVFFLGTALLAWPHNVWLRNFFISPTF
                     LFALLIFLPLMMEISQGFKVLSLRQLAVILLLTFAISGAKGNFFPVIGAALLATTVYA
                     VLFRQRVMPVVIILLSSLAVFAVVFLLIFKGTGSEGINILPLTIIKTTALYQIVEKKM
                     ATLHPGWFPLVTIPLYLILFYSFRLPVLLRSLWQLLRRPKEIEAKQVFLLALVLAGCI
                     PAYFLNYRGTSQYYFLLAGWAALNLMAAGYLTELFSNRSRHLLKIVMILLVLLSVGDT
                     LSTMQYQAYSLQKHMELRNKPLTPAMYQGLSYLRTGTPSDAVVASFRTFWLNKDNPRF
                     FYYSAFAQRRMVVEGWMYMRPEYQSQAQIRYADMQTLFYTRDQKMARQMIEKYGVDYL
                     LVDKSHRQKLRFDSSLFLEKVYCNDQVELYKYAK"
     gene            2402..>3183
                     /locus_tag="P5549_10515"
     CDS             2402..>3183
                     /locus_tag="P5549_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRW13539.1"
                     /translation="MGVSRREFFKIGGGGVAVALGAGLAGKSLLEEPHSDALAAAPAK
                     VVYKHGVCGICELNCAMKGKIENGRLVKLEGKAVDQHSRGSLCAKGNSGINMLYDPDR
                     LKSPLIKTVKGRVPGQDPQWKKATWDEAISQAASAIKKAIDEHGPQSVIWIGHHKGVD
                     FLNAIGSPNNISHQSTCNNVRQLATQLVLGTANYVPDFESSKYILCFGWDQLGKAKNS
                     FARGPMHAKNENGAKLVVFDPRLSTTAAKADEWIPIRPGTDLA"
BASE COUNT          747 a          764 c          804 g          868 t
ORIGIN      
        1 gacccgtcac aatctgcagc aggaactgct gcgcatcaaa gacaaactgg ccactacggt
       61 gttgtttgtg acccatgata tcgatgaagc tctggtgttg ggcgacagaa tcctaatcat
      121 gaccagaaac gggcaagtca ggagtatttt cgccaacccc ctggccaaac cgcgccagcc
      181 gagaatggaa ggctttgccg ggatgtgggg caagatctat gatcaattgg gggagagttg
      241 aggcaacgac ataattcgtt cagggaggaa gatggtgact gcagcacctt tgaaacgtta
      301 tcttttggct gttctggcgg ttgtcaacct gctggtcttg ttttattttg gcctgggggc
      361 ttttgacctg ctgaaactct cggctacagt tgccgtgggg acattgatcc ccgggctttt
      421 gttgctgcat tggtggaaac tctatccgga tacccagtat cgctttgttt tggcgctggt
      481 catcggaatg tctctcgata ttttgctgtt tatcggggcc tcctggctga agctgcattt
      541 tctcatttat ctcctgccgg ggataggatt gatgctttac ctgcaaaaca ggctttataa
      601 aaaagacgga cgcagtatca aaaatgcctt ccaagccttt tctgtctggc atttgaccgt
      661 cttgactgcg atttccttat tggtagtggg cttgatcgtg tggttttttt atattcccaa
      721 ccaattgcca ggttcccagg cagtcgtata ccatgttgac gatccctggc atctgggtaa
      781 catcgccgaa atcttgcatc actggtaccc ccaggacccg cgcctggctg gaatggaatt
      841 caactaccac atcttttact atgtttatat ggcctttctc gcctttataa gcaaagtaag
      901 cctgccggtc atttattttc gcttttatat tattttattt ctttatacct tgatgggcgg
      961 tgcgtatttc gttgccagcc gctggtttca tgaccggagg gcgggcatcc tccacctggt
     1021 catcgtcttt ttcctgggga cggcactgct ggcctggccg cataacgtat ggctgcgcaa
     1081 cttctttatc agtcccacct ttttatttgc cttgcttatt tttttgccgc tgatgatgga
     1141 aatcagccag ggtttcaaag tgctcagcct gcggcaactg gctgtcatct tgctgctgac
     1201 atttgccatc agtggtgcca aaggcaattt tttcccggtc atcggggctg ctttgctggc
     1261 aacgacagtt tatgcagtgc ttttccggca aagagtaatg ccggtggtga taatactgct
     1321 cagcagtctg gctgtttttg ccgtcgtctt tctgttgatt tttaaaggta ccggcagtga
     1381 agggatcaat attctgccgt tgaccatcat caagacgact gccctttatc agattgttga
     1441 gaaaaaaatg gcaaccctgc atccgggctg gtttcccctg gtgacgattc ctttatacct
     1501 catcctgttt tacagtttcc ggctgccggt gctgctgcgt tcgctctggc aattgctgag
     1561 aaggccaaag gagatcgaag caaaacaggt gtttttatta gccttggtgc tggctggttg
     1621 tatccccgcg tatttcttga actaccgggg tacttcccag tattattttc tgttggcggg
     1681 ttgggccgct ctgaacctga tggccgccgg atacctgaca gagctttttt ccaaccgcag
     1741 ccggcatctg ctgaagatcg ttatgattct gctggtgctc ctgtcggtcg gtgacaccct
     1801 ttcaaccatg caataccagg cctacagctt gcaaaaacac atggaactgc gcaacaagcc
     1861 gctcacgccg gccatgtatc aaggcctctc ctatctgcgc accgggacgc cgtccgacgc
     1921 agtagttgct tccttccgta ccttttggct gaacaaggac aatccgcgct ttttttatta
     1981 ttccgccttt gcccagcggc gtatggtagt ggagggatgg atgtatatgc gtcctgagta
     2041 ccagagccag gcgcagattc gctatgcaga catgcaaacc ctcttctaca ctcgcgatca
     2101 aaagatggcc cgccaaatga ttgaaaaata tggcgttgac tatctcctcg tcgacaaatc
     2161 ccaccgtcag aaactgcgct ttgattcctc gttgttcctg gaaaaagtct attgcaacga
     2221 tcaggttgaa ttgtataaat acgctaaata attctggttc aataaaagcc agctaaaagc
     2281 gtggcttata acccgataac aaagtcagat aattgtcggt aatggtcgag tgctatagtt
     2341 agaatagaga aacattataa agaaggaaaa agattggaag cgacacaggg gagggaaacg
     2401 tatgggtgta tcaagacgtg aattttttaa aatcggcgga ggcggcgtgg ctgttgcact
     2461 aggtgccggt ctggccggca aaagtctgct tgaagaaccc cacagcgatg cgctggcggc
     2521 ggcgcctgcc aaagtagtat ataaacacgg ggtatgcgga atttgcgaac tgaactgtgc
     2581 gatgaaaggc aagattgaaa acggccgttt ggttaaactg gaagggaaag ccgttgacca
     2641 acacagccgc ggttctttat gtgccaaagg caattccggt ataaatatgc tttatgaccc
     2701 ggatcgatta aaatctcccc tgattaaaac tgtcaaaggg agagtgccgg ggcaggatcc
     2761 ccaatggaaa aaagctactt gggatgaagc catcagccag gctgcatcag caattaagaa
     2821 ggccattgac gaacatggtc cccagtcggt catatggatc ggccatcaca agggggtgga
     2881 ctttttaaat gccattggca gccccaacaa tatcagtcat caatccacct gcaacaatgt
     2941 tcggcagttg gcaacccaac tggttcttgg tacggccaac tatgttcccg actttgaaag
     3001 cagtaaatac atcctctgtt ttggctggga ccaattaggc aaagccaaga attcttttgc
     3061 gcgcggaccg atgcatgcta aaaatgaaaa cggagccaaa ttagtggtat ttgatccccg
     3121 cttgtcaacg accgcagcca aagccgatga atggattccc attcgtccgg ggactgatct
     3181 ggc
//
LOCUS       DAOTHZ010000151         3097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_37259_length_3097_cov_6.628205, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000151 DAOTHZ010000000
VERSION     DAOTHZ010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37259_length_3097_cov_6.628205"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="P5549_10520"
     tRNA            complement(1..75)
                     /locus_tag="P5549_10520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:cgt)
     gene            complement(161..871)
                     /locus_tag="P5549_10525"
     CDS             complement(161..871)
                     /locus_tag="P5549_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRW13540.1"
                     /translation="MQTVAKTTAVSLVSQVAAGICQGIKNPLSTVRQFLQLFESKEKY
                     KADKKAYELVIEELDRAEDIISTFSLLACKKDEIMKLQSLNDNIVQVFPLIMQEAEKN
                     KVFIKLELSEAAPIMMDESEIRQLLHNLVRNGMEAMQNGGILTVGTFQDKNGVNLVVK
                     DKGTGIPEDVMEKIATPFFTTKERRIGLGLSVCYSIVDRHNAQIDIKSSPEGTCVNVV
                     FPAAAKAANSQTGKSAPA"
     gene            complement(823..1029)
                     /locus_tag="P5549_10530"
     CDS             complement(823..1029)
                     /locus_tag="P5549_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13541.1"
                     /translation="MMTNTPDIIDKDRLIEEMKAEVLRLQIENTQLRRELGNLTFRGV
                     SEQIKTENECKQSQKLLLSLWSAR"
     gene            complement(1225..1503)
                     /locus_tag="P5549_10535"
     CDS             complement(1225..1503)
                     /locus_tag="P5549_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13542.1"
                     /translation="MFISKFDSVDGKPDEDQIQTWVEGYFQNMITTLNSFFVHVSAAE
                     AVERMSAVPFAQLVSEELEGESETVIQLAVGIINELAATELEFMRAYL"
     gene            complement(1490..2881)
                     /gene="argH"
                     /locus_tag="P5549_10540"
     CDS             complement(1490..2881)
                     /gene="argH"
                     /locus_tag="P5549_10540"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRW13543.1"
                     /translation="MKLWSGRFTKQTDQLTEDFNASIAFDSRMYAQDIRGSMAHAAML
                     AQQGIISREEGEQIIAALQEIQQEIAEGRLQFTVEAEDVHMNIETFLTAKIGNVGKKL
                     HTARSRNDQVALDVRLYLNDEMESIRELLLELAGTLLQISSEHRETIMPGYTHMQKAQ
                     PITLAHHLLAYFEMLRRDVERLDDCRKRVLAMPLGSGALAATGFNLDRDRVRTELGFA
                     SLTRNSLDGVSDRDFAIEFCSFASILMMHLSRFSEEIILWSSDEFSFVQLDDGFSTGS
                     SIMPQKKNPDIAELVRGKTGRVYGDLITLLTVMKSLPLAYNKDMQEDKEALFDAIDTV
                     KKCLLVFSPMLRTCRFQKENMAAGARGGFTNATDLADYLVGKGLPFRDAHAVVGQAVL
                     YCVEQKKTLEELELETFQTFSERIGPEVYEHLDILNCVAKRSIPGGPAPETVKKAQRE
                     AAQWLERMQHVHQ"
     gene            complement(2960..3088)
                     /locus_tag="P5549_10545"
     CDS             complement(2960..3088)
                     /locus_tag="P5549_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRW13544.1"
                     /translation="MREMSSRMPRDQEEMLSISGVGENKWKKYGRQFLEVIRSYIK"
BASE COUNT          702 a          791 c          733 g          871 t
ORIGIN      
        1 tggagccgat gggcggattt gaaccgccga cctgctgatt acgaatcagc tgctctgcca
       61 ctgagccaca tcggcataag gttaaaataa ctcggggctg accccgagtg attgtttacg
      121 ccatgataaa taataacaag attgtgctct gccgtcaaga ttaggcggga gcagacttgc
      181 cggtctggct gttggcggcc ttcgctgccg ccggaaatac gacgttaacg cacgttcctt
      241 cgggactgct tttaatatca atctgagcat tgtgtctgtc aacaatgctg taacaaacag
      301 acaatcccaa gccgattcgc ctttccttgg tggtaaagaa gggggtggcg attttttcca
      361 tgacatcctc ggggataccg gttcccttgt ctttcacgac cagattgaca ccatttttgt
      421 cctggaaggt gcctacggtt aaaatacctc cgttttgcat ggcttccatg ccgttgcgca
      481 ccagattgtg cagtagctgc ctgatctcac tctcatccat cataatgggt gcagcctcac
      541 ttagctccaa tttgataaac actttgtttt tttcggcctc ttgcataatc aggggaaata
      601 cttgcacaat attgtcattc aaactttgca atttcatgat ttcatccttc ttgcaggcca
      661 gcaatgagaa agtggaaatg atatcctcgg cgcggtcgag ttcttctatg acaagttcat
      721 aagctttctt gtcagctttg tatttctctt tgctctcgaa cagctgcagg aattgccgca
      781 cggtcgacag gggatttttg attccctgac aaatacctgc ggctacctgg ctgaccagag
      841 agacagcagt agtttttgcg actgtttgca ttcattttcg gtttttattt gttcactgac
      901 acccctgaac gtaaggttgc caagttccct tctgagctgg gtattttcga tctgcagccg
      961 taaaacttcg gctttcatct cctcaattaa gcggtctttg tctattatat ccggggtgtt
     1021 tgtcatcatt attctcgcct tccgttattt tccacaatta gtatcaacct cataattata
     1081 tgaaaaataa gttttttggt ttagggttat gtgtcagcgt ttgtcgaggt gtaaaaatat
     1141 ttttaatgtt cagcgcttaa aagcaaaaaa ccctgctttt ccaagcaagg ttctccatga
     1201 tctgtgatta tttttagcaa tcaatcatag ataagcgcgc atgaactcca gttcggtggc
     1261 ggccagttcg ttgatgatgc ctactgccaa ttggatgacc gtttcactct ccccttccag
     1321 ttcctcgctg accaactggg caaaggggac ggcggacatg cgctccacgg cttcagccgc
     1381 gctcacatgg acaaaaaaac tatttaatgt cgtaatcatg ttctgaaaat acccttcgac
     1441 ccaggtctgg atctgatctt catcgggttt tccatcgacg ctatcaaatt tactgatgaa
     1501 catgttgcat cctttccagc cattgggcgg cttcgcgttg ggcttttttt acggtttccg
     1561 gtgccggtcc gccagggatg ctgcgtttgg ccacacagtt caggatatcc aagtgctcat
     1621 agacctctgg gccaatccgc tcggaaaagg tttgaaacgt ttccagctcc aactcttcga
     1681 gggtcttttt ctgttcaaca caatacaaga cggcctggcc gactacggca tgagcgtcgc
     1741 ggaagggcag tcccttgccg accagataat cggccagatc agtagcattg gtaaatccgc
     1801 cccgggctcc ggcagccatg ttttccttct gaaagcgaca ggtcctgagc atggggctga
     1861 aaaccagcag acattttttt acggtatcaa tggcatcaaa caaggcttcc ttgtcttcct
     1921 gcatatcttt attgtaggcc aggggcagcg atttcatcac cgtcagaagg gtgatcagat
     1981 caccgtagac gcgcccggtc ttgccccgca ccagttcagc aatgtcaggg tttttcttct
     2041 gcggcatgat gctggaacca gtggaaaatc cgtcgtccag ctgcacaaag gaaaactcat
     2101 cgctggacca aagaatgatt tcctcagaaa aacgcgacag atgcatcatt aatatactgg
     2161 cgaaactgca gaattcaatg gcaaaatcac gatcactgac gccgtccagg ctgttacggg
     2221 tgagggatgc aaaccccagc tcggtacgca cacggtcacg gtccagattg aaccccgttg
     2281 ctgccagggc accggaacca agcggcatgg ccagcactcg tttgcggcag tcatcgagcc
     2341 gctccacatc gcggcgcagc atctcaaaat aggccagcag atgatgggcc aaggtgatgg
     2401 gctgggcctt ttgcatatgg gtatagccgg gcatgatggt ttcccggtgt tcgctgctga
     2461 tctgcagcaa agtcccggcc agttccagca gcagttcgcg gatgctttcc atctcatcgt
     2521 tcagataaag ccgtacatcc agtgccacct gatcattgcg gctgcgcgcg gtgtgcagtt
     2581 tcttgcccac attaccgatt ttggcggtca ggaatgtttc gatattcata tgcacgtctt
     2641 cggcttccac cgtaaactgg agccggccct cagcgatttc ctgctggatt tcctgcaagg
     2701 cggcgatgat ctgttcccct tcttcacggc tgatgattcc ctgctgggcc aacatggcgg
     2761 catgggccat gctgcctctg atatcctggg catacatgcg gctgtcaaag gcgatggaag
     2821 cattaaaatc ctcggttaat tgatcggtct gtttggtgaa acgaccactc caaagtttca
     2881 tattacacct caaaattttt agtaattttt attatattat tttcctgctt gaaatgcatc
     2941 tctattgaga ataccgtgtt tattttatat agctgcggat aacctcgaga aactgccggc
     3001 cgtatttttt ccatttgttt tcgcctacgc cggagataga cagcatctcc tcctgatcac
     3061 ggggcatccg gctgctcatt tcgcgcaggg tgctgtc
//
LOCUS       DAOTHZ010000152         2972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_39354_length_2972_cov_7.171752, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000152 DAOTHZ010000000
VERSION     DAOTHZ010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39354_length_2972_cov_7.171752"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="P5549_10550"
     CDS             <1..514
                     /locus_tag="P5549_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRW13545.1"
                     /translation="AVLLAILLGPWPACIGISVALIIQALIFGDGGVIAIGANCFNMA
                     VVMPWIGYLTYQLIRGKSDFLSTRAIAAAFIAGYIGLNAAALVTALEFGSQYHWFQAV
                     DGTPLYFPYSVKVAVIAMMSEHLLFAGPVEGMVTALGTWFVARNYPALLTKKLTKRIE
                     PDEMGVDNLV"
     gene            507..821
                     /locus_tag="P5549_10555"
     CDS             507..821
                     /locus_tag="P5549_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HRW13546.1"
                     /translation="MFKNFKKLWMALGAFILLSPLGLIATGTAFGEWGTDQLPEEVGF
                     VPAGLAKFAEFWSSSPLPDYGVPGWDGSFVQSAGGYVLSALVGAGLVILLISLFNKIV
                     KE"
     gene            921..1796
                     /gene="cbiQ"
                     /locus_tag="P5549_10560"
     CDS             921..1796
                     /gene="cbiQ"
                     /locus_tag="P5549_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HRW13547.1"
                     /translation="MPGNKSIYLKKSLQYMRKTICEDRQTEYYARCNGALQKISPSNK
                     LLGMLGLILVISISRQIPILLGVWLLVLILMRASRLPVWRLQIRSWGVIPLLSLLFSL
                     PVMFNVVMDGAPLFYLRYSSLTIPWLGINWTEGIFITRQGFTAAIFLFLRVGLSLSLG
                     ILLVMTTPVADIFQSLRVIRVPDLFIMIMEMTYRYMMVLLSVSIEMFDARVLRTVGKL
                     PPKWQRAQVGSSIASLFARSVALSEEVYQAMIARCYTGEVLVSSEEKAHWPDGLPGTI
                     VFEQNNEWCKAGERK"
     gene            1793..2584
                     /locus_tag="P5549_10565"
     CDS             1793..2584
                     /locus_tag="P5549_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRW13548.1"
                     /translation="MTVMELDKISFRYEHEEWLFNNMSLTIEAGESICLLGANGSGKS
                     TLLKLFCGLVFPSTGIYRAFEQEITEDLLEDNRFAQGFHRRIGFIFQNSDAQLFTSRV
                     WDEVAFGPLQMGFSRAEVKRRVDDVLVMLNLQNLAGRSPHRLSGGEKKKVAVASTLVL
                     NPEVIILDEPTNGLDPKTQRWMVELLMELNKHGRTIITSTHNLDLAHAISQRALVLSE
                     EHCLIYDGDSHKALEDKQLLMAVNLIDEYCHVHGESQHSHLYKHG"
     regulatory      2711..2833
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT          737 a          584 c          786 g          865 t
ORIGIN      
        1 agcagtactg ctggctatct tgttgggtcc atggccggcg tgtatcggga tatccgtagc
       61 cttaatcatt caggctttga tttttggtga tggaggggtc atcgcgatcg gtgccaattg
      121 tttcaatatg gcagtggtga tgccttggat tggttattta acttatcaac tgatcagagg
      181 aaagtctgat tttctttcga cccgggccat tgcagctgcc tttattgccg gatatattgg
      241 attaaatgct gcagccttgg tgacggcgct ggaatttgga tcccaatacc attggttcca
      301 ggccgttgac ggaacgccgc tttattttcc ttattcggtc aaagttgccg tgattgccat
      361 gatgagcgag catttattgt tcgccgggcc ggtagagggg atggttactg ccttgggaac
      421 ttggtttgta gccaggaatt acccggcgtt actgacgaaa aaactgacga aaagaattga
      481 acctgacgaa atgggggtgg acaaccttgt ttaagaattt taaaaaactg tggatggctt
      541 tgggcgcatt cattcttctc agtcctctgg ggctgattgc tacgggaacg gcatttggag
      601 aatggggaac ggatcagctg ccggaagagg ttggttttgt tcccgcaggc ttggccaaat
      661 ttgctgaatt ttggagcagc agccctttgc ctgactatgg cgtaccgggt tgggacggaa
      721 gctttgtcca gtctgccgga ggctatgttt tatctgccct ggtgggggct ggactggtga
      781 ttttgttaat cagtcttttc aacaaaatcg ttaaagaatg atcttttccg aagctgtggg
      841 aattaaaacc gggaaaggaa atagatatgt tactgcctga ttggatgata gaggagcaaa
      901 atcagattgc agttgctgtt gtgccgggaa acaaaagcat ctatttgaag aaaagtttgc
      961 agtatatgcg caagacaatc tgtgaagacc ggcaaaccga gtattatgcc cgctgcaacg
     1021 gagcactgca gaaaatcagc ccgtccaata agctgcttgg catgttggga ttgattctgg
     1081 tcatcagtat aagtcgtcaa attccgatat tgcttggggt atggctgctt gtgctcattc
     1141 ttatgcgggc atcccgcttg ccggtttgga ggctgcaaat aaggagctgg ggcgttatcc
     1201 cattgctcag cttgttattc agtctgccgg tcatgttcaa tgtagtaatg gatggtgcgc
     1261 cgcttttcta tcttcggtat tcatctttga ccatcccatg gctgggcatc aattggacgg
     1321 aaggaatttt cattacccgg cagggattta cagcagcaat atttttattc ttacgtgtcg
     1381 gactttctct ttccctggga atattattgg tgatgacgac acccgttgca gatatttttc
     1441 aatcactgcg ggtcattcgt gtccccgatt tgtttatcat gatcatggag atgacgtatc
     1501 gttatatgat ggttctgctg tctgtttcca tcgaaatgtt tgatgcccgg gttcttcgta
     1561 cggtgggaaa gttgccgcca aaatggcaaa gggctcaagt gggatcgtcg atagcatcac
     1621 ttttcgcccg tagtgttgcc ctctcggagg aagtctacca ggccatgatc gcacgctgtt
     1681 atacaggtga agttttggtc tcgtcagagg agaaagcgca ttggccagat gggttaccag
     1741 gaacgattgt gtttgaacaa aataatgaat ggtgcaaggc aggagaaaga aaatgacggt
     1801 tatggaactg gataaaatta gtttccgtta cgaacatgaa gaatggttat ttaataatat
     1861 gagtctgacg attgaggcgg gagagagcat ttgtctgctg ggggccaatg gttcaggcaa
     1921 atcgacgctg ctaaagcttt tttgcggttt ggtgtttcca tcgaccggaa tctaccgcgc
     1981 ttttgaacag gagattacgg aagatctgtt ggaagacaac cgttttgccc aaggattcca
     2041 ccgacgtata ggttttattt ttcaaaactc ggatgctcag ctgtttacca gccgggtatg
     2101 ggatgaagtt gcctttggcc cgttacagat gggattcagc cgagccgagg tgaagcgcag
     2161 agtggatgat gttctggtca tgttaaatct gcaaaacctg gcaggaaggt ctccccatcg
     2221 gctcagtggc ggggaaaaga aaaaagttgc agtggcatct actttggtcc tgaatccgga
     2281 agtcattatt ctggatgagc caaccaacgg acttgatccc aagacccagc gctggatggt
     2341 ggaactgttg atggaattaa acaagcatgg gcggacaatt attacctcca ctcacaatct
     2401 ggatctggcc catgccattt cccagcgggc cttggtgttg tccgaagaac actgcctgat
     2461 ttatgatggc gattcccata aggcactgga ggataagcaa ctattaatgg cggttaattt
     2521 gatagatgaa tactgtcatg ttcatggcga gagccagcac agccatcttt ataagcacgg
     2581 ttaattattc aaccgctaga atttaatgtg aaatcttctt taaattataa tttgctgata
     2641 aatatttcta attaaataaa gcaggatttt tgcgattctt cacgaataac acatgtaatt
     2701 acgacacaaa tttcaatatc atctccgagt ctgtcatact aaggcgatga acgctatggt
     2761 gacgaggcca ttgggtatag ttggaaacgg ctgtgccttc cattgcgaaa aggagtgctc
     2821 aggtcaggcg ataaaagtgt gttcatgaag aatacacact ttgtttgtgc ttttattgct
     2881 gcccggcatc tttttgcagg gcagctttgt tgtatttaaa attaaatatc atctccgagt
     2941 ctgtcatact aaggcgataa acgctaaggt ga
//
LOCUS       DAOTHZ010000153         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_41490_length_2857_cov_5.224839, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000153 DAOTHZ010000000
VERSION     DAOTHZ010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41490_length_2857_cov_5.224839"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1313
                     /locus_tag="P5549_10570"
     CDS             21..1313
                     /locus_tag="P5549_10570"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRW13549.1"
                     /translation="MKLLIKGGRVIDPANEINDVLDVLIEDGRIKTVDRNIKAADAET
                     IDATGKLVCPGFIDMHVHLREPGFEYKEDIASGTRAAAAGGFTTVCCMPNTDPVIDNA
                     AVASFVRERARKSGVVNVLPIGTISKGQEGKELSEMAELMEAGCVAVSDDGRPVLDSG
                     LMRYAMQYATMFDLPLLSHCEETSLSADGQMHEGYYSTIYGLKGIPAAAEEVMVARDI
                     ILAEATGARVHICHVSTAGSIELIRTAQDRGVQVTCEVTPHHLLLTDEVVGGYDADTK
                     VSPPLRSLNDVIALRDGILDGVVDCIATDHAPHHRESKDCEFNLAANGISGLETAVAL
                     IMDQLVIPGIIDEETMVRLFTIGPADILGLTKGTLEAGADADVTIIDPQLSQTVDPEK
                     FYSKGKNTPFKGMSLRGWPCMTIVQGKVVCRDGIIVNG"
     gene            1335..2432
                     /gene="carA"
                     /locus_tag="P5549_10575"
     CDS             1335..2432
                     /gene="carA"
                     /locus_tag="P5549_10575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRW13550.1"
                     /translation="MKGYLVLENGRVFQGSLLNECRSAGGEVVFTTAMIGYQDVITDP
                     SYYGQIVVMTYPLVGNVGFNESSFASRRAMVEGLILREATAFPSHWEMDTDMITFLND
                     SQVTVLTDVDTRALTRVIRNEGTMGGIITARLDEIEKLTAAARQASARLSGDLVRQVT
                     RREIEHFGDGGKRIVMLDLGSKRGVLNSFLQRGCEVAAVPAASSAEEIMSLQPDGLFV
                     SDGPGNPQSLGYAIATVKSLLDRLPVFGVGLGHQVIALAMGAEINKLAYGHRGSNHPV
                     KNLLNGRVYITTQNHGYAIDEISLAGTDLQVFLRSLNDHTVEGVSHRRLPVFSVQFDP
                     EGYFGYSDTGIFFDRFVNSLQVGHGNNVIGG"
     gene            2436..>2857
                     /locus_tag="P5549_10580"
     CDS             2436..>2857
                     /locus_tag="P5549_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRW13551.1"
                     /translation="MPLIEGLKKVMVIGSGPIIIGQAAEFDYAGTQACRALKEEGIEV
                     VLLNSNPATIMTDTNIADHVYIEPITIATVSKILAKENPQGIIATLGGQVGLNMALAL
                     DREGILARTGVPLLGTPLQAIARAEDRELFKQTMEEIG"
BASE COUNT          661 a          738 c          861 g          597 t
ORIGIN      
        1 gatgatggga ggcacaaaag atgaaattgc taattaaggg cggcagagtg atcgacccgg
       61 ccaatgaaat caatgatgtg ctggacgttt tgatagagga tggcaggatc aaaacggtgg
      121 acaggaatat aaaggcggct gatgcggaaa ccattgatgc cactggcaaa ttggtctgtc
      181 cggggtttat tgacatgcat gtccatctgc gcgaaccggg ctttgagtac aaggaagaca
      241 tcgcctccgg tacccgggcg gcggctgcag gcggatttac gacggtatgc tgcatgccca
      301 atactgatcc ggtcatcgac aatgctgccg tggccagctt tgtgcgggaa agggcccgta
      361 aatccggagt ggtaaatgtc ttgccgattg ggaccatcag caaaggccag gagggcaagg
      421 aactgagcga aatggcggaa ctgatggaag ccggctgcgt agctgtctcc gatgatggca
      481 gaccggtgct ggattcggga ttgatgcgct atgccatgca gtacgcgacg atgtttgatc
      541 tgccccttct atcccactgc gaagagacca gcctgtccgc tgacggacag atgcatgaag
      601 gctattattc caccatttat ggtttaaaag gcatcccggc agccgccgag gaagtcatgg
      661 tggcacggga catcattctg gcggaagcaa ccggcgcgag agttcatatc tgtcacgttt
      721 ccaccgccgg ctccattgag ctgattcgta ctgcccagga tcgcggcgtg caggtaacct
      781 gcgaagttac gcctcatcat ttgctcctca ccgatgaggt ggtaggcggt tatgatgccg
      841 acaccaaagt cagcccgccg ctgcgcagtc taaatgacgt aattgcgctg cgggacggga
      901 ttttggacgg agtggtggat tgcattgcca ccgatcatgc cccccatcac cgggaaagca
      961 aggattgcga attcaatctg gcggccaacg ggatttccgg actggaaacc gcggtggcgc
     1021 tgatcatgga ccaactggta attcccggga tcattgatga ggaaaccatg gtacggctgt
     1081 tcaccatcgg cccggcggat attcttggcc tgaccaaggg caccctggag gcgggggcgg
     1141 atgcagatgt caccatcatc gatccccagc tgagccaaac ggttgatccg gaaaaattct
     1201 attcaaaagg caaaaacacc ccgttcaagg gcatgagcct gcgcggctgg ccctgtatga
     1261 ccatcgtcca gggcaaagtc gtttgccgcg atggcatcat cgtcaacggg taatgataga
     1321 acagggaggt tcctatgaaa ggatatttgg tactggaaaa cgggcgggtg tttcaaggct
     1381 cgctgctgaa tgaatgccgc agtgccggcg gcgaagtggt tttcaccacc gccatgatcg
     1441 gttatcagga cgttatcacc gatccttcct actacggtca aattgtggtc atgacctatc
     1501 cgctggtcgg caatgtgggt ttcaacgaaa gcagtttcgc ctcccgccgg gctatggtgg
     1561 aaggcctgat cctgcgggaa gcgaccgctt ttcccagcca ttgggaaatg gatacggaca
     1621 tgattacctt tttgaatgac agccaggtca cggtgctgac cgacgttgat acccgggctc
     1681 tgacccgcgt gattcgcaat gaaggaacca tgggcgggat catcaccgca cgcctcgacg
     1741 aaattgaaaa actgacggct gctgcccgcc aggccagtgc cagattgagc ggtgatctgg
     1801 tccggcaggt tacccgccgg gagattgaac acttcggcga cggaggaaaa cgtatcgtca
     1861 tgctcgatct gggcagcaag cgcggagtgc tgaactcctt cctgcagcgg ggctgtgaag
     1921 tggcagctgt gcctgccgcc agcagtgcag aggaaatcat gagcctgcag ccggacggtt
     1981 tgtttgtctc cgacggtccc ggcaaccccc agtcgctggg atatgcgatc gccacggtga
     2041 aaagtctgct cgaccggttg ccggttttcg gggtaggact ggggcaccag gtgatcgctc
     2101 tggccatggg ggcggagatc aataaactgg cctacggtca ccggggcagc aaccatccgg
     2161 tgaagaatct gcttaacgga cgggtctata tcaccaccca gaatcatggt tacgccattg
     2221 atgagatctc cctggccggc acggatctgc aggtatttct gcgcagtctc aacgaccaca
     2281 ccgtggaagg tgtcagccac cggcgactgc cggtattctc ggtacagttc gatccggaag
     2341 gctatttcgg ctacagtgat accggcatct tttttgatcg ctttgtcaac agtcttcagg
     2401 ttggccacgg caacaacgtc ataggaggat aaggaatgcc attaatagag ggacttaaaa
     2461 aagtgatggt gatcggttcc gggcccatca tcatcggcca ggcggctgag tttgactatg
     2521 ccggtaccca ggcttgccgg gccttgaaag aggaaggcat tgaagtggtg ctgttaaaca
     2581 gcaatccggc taccatcatg accgatacca atatcgcgga tcatgtctat attgaaccca
     2641 tcaccattgc caccgtcagc aagatcctgg ccaaggaaaa tccccaggga ataatcgcca
     2701 ccctgggcgg acaggttggg ctgaacatgg ctttggccct ggatcgggaa gggatcctgg
     2761 cgcgaaccgg ggtgccgttg ctgggaacgc ctctgcaagc cattgcccgc gccgaagacc
     2821 gggaactttt taaacaaacc atggaagaaa tcgggga
//
LOCUS       DAOTHZ010000154         2839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_41809_length_2839_cov_6.807112, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000154 DAOTHZ010000000
VERSION     DAOTHZ010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41809_length_2839_cov_6.807112"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..1739
                     /locus_tag="P5549_10585"
     CDS             384..1739
                     /locus_tag="P5549_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRW13552.1"
                     /translation="MNMKKSFTEEYNSKLTSADEAVKIINSGDQLFYGEFTMFPEALD
                     EALAKRTEEVWDVHVRGICYTKVPKIVLADPAQEHFVLEDWHCGVVSRKLAEKHLSAY
                     LPITYHQGPRIVKKYERVNAALIMVSPMDENGYFNTGPTNSTTSAYVDKAMKVIVEVN
                     NKVPICLGGNGESLHISQVDYVVEGNNPPLLQLPASVPSECDYTIADYIMEEIHDGAC
                     IQLGIGGLPNVIGAKIAASDLRDIGFHSEMLVDSCVDMSEAGIMTGARKSIDKNKMVY
                     TFAMGTDRLYEFLHNNPKCASYPVNYVNDPRMIALNNNVIAINNAVEIDLFSQVCSET
                     AGIKQISGTGGQLDFILGAFNSHGGKGMICLGSTYKDKDGVVHSRIKPTLTPGVVVTV
                     PRSIVQYIVTEYGIVQMKAKSIWDRADALIHIAHPDFRDDLVKAANEMNIWTRSNRIV
                     E"
     gene            complement(1850..>2839)
                     /locus_tag="P5549_10590"
     CDS             complement(1850..>2839)
                     /locus_tag="P5549_10590"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRW13553.1"
                     /translation="PVLRCKTESFWQYIHIRNLEARVRIMGWRDVSEEEILLANIEKY
                     NNEHILSYYRDFKEPRYAYAEYDVIFSAIVQMLATRSEQKLRAVDVCGGAGKSAFILK
                     KLVPQAEVTLVDLSEKMLEIARQRSCEEKIGDIEMIQEDAVTFLQRDRQFDIIVFSSA
                     IHHFKDPVTLLNSAAQRLSEQGVIITIAEPNTLTKTKRYKSLAFLFCCWADKMTIMKR
                     TIHRGMVFFTGEAAAAADDFDIAEYQAYKGIDDLQLEHDLQAAGLNTLLHMQYPAGGD
                     PFMTQIMSLFKLHWAFCLVLTKQPWDDQKQVSDGLQSMISQGLPYKYICPLPE"
BASE COUNT          786 a          683 c          607 g          763 t
ORIGIN      
        1 ttagtgcaaa gcaggtaata gttgtaaatt attttatctc aatacttttt ccaacagcca
       61 gttaattaag aattaatgca aaatcgcatt atttttgcgg gttatccttg gctggcagta
      121 aaaaattaat ttcaactggc agaaaatttt atgacaggat aagcagaaga attattaatt
      181 actgcaattg ctgtagcgat tttcgacatt atcatgatga acccttatat taatgcaggg
      241 tgtaatagcc attattatat taagctattg cataatatat cattctatat taaattattc
      301 tataataatt tatatacttt ggtttcgtaa aatctaattg ttaaatttta taaaacaata
      361 ttcaacaaaa aggcggcgat caaatgaaca tgaaaaaaag ttttacagaa gaatacaaca
      421 gtaaactcac cagcgctgat gaagccgtta aaatcatcaa ctccggcgac caattgtttt
      481 acggagaatt taccatgttc ccggaagcgc ttgatgaagc cctggcgaaa cggacagagg
      541 aagtatggga cgtgcatgtc agagggattt gttacaccaa ggtcccaaag attgttctgg
      601 ccgaccctgc ccaggaacat tttgtcctgg aagactggca ttgcggggtg gtatcacgca
      661 aactggctga gaaacatctg tctgcctacc tgcccatcac ttaccatcag ggaccgcgca
      721 tcgtaaaaaa atatgagcgc gtcaatgccg ctttgattat ggtatccccc atggatgaaa
      781 acggctattt caacaccggt cctaccaact ccaccacctc ggcctatgtt gacaaagcca
      841 tgaaagtcat cgtcgaagtc aacaacaagg ttcccatctg tctgggcggc aatggagaat
      901 cgctgcacat ttcccaggtg gactacgtgg tggaaggaaa caatccgcct ttgttgcaat
      961 tgcctgcctc tgttcccagc gaatgcgatt ataccatcgc tgactatatc atggaagaaa
     1021 ttcatgacgg ggcttgcatc caactaggga tcggcggcct tccgaatgtc attggcgcca
     1081 agattgcggc cagcgatctg cgggacattg gttttcattc cgagatgctg gtcgactcct
     1141 gcgtggacat gtcagaagcc ggcatcatga ccggtgcccg caagagcatc gataaaaaca
     1201 aaatggtcta tacctttgcc atgggtacag accggctgta tgaattcctg cacaacaatc
     1261 ccaagtgtgc ttcctatccg gtcaactatg tcaacgatcc gcgcatgata gcgttgaaca
     1321 ataacgtcat cgccatcaac aacgccgtag agatcgatct tttcagtcaa gtgtgttcag
     1381 agaccgcagg catcaaacag atctccggca ccggcggaca gctcgacttt attctgggcg
     1441 cgtttaattc ccacgggggc aaaggcatga tctgtctcgg ctccacctac aaagacaaag
     1501 acggcgttgt ccactcccgt atcaagccca ctctcacccc gggcgtggtc gtgactgtac
     1561 cccgttccat cgtgcagtac atcgtgacgg aatacggcat tgtccagatg aaagccaaat
     1621 ccatctggga tcgggctgat gccttgattc atattgctca tcctgatttc cgcgatgatc
     1681 tggtcaaggc cgccaacgag atgaacatct ggacccggag caaccgaatc gtggagtaaa
     1741 tttaaacaac ccctctcaag aaagacgccg caggctcatt ctgcggcgtc tttttggcaa
     1801 ttaagtttgt ttatgcgaga taccgggctt tgatcataat ggttatcatc tattctggta
     1861 aaggacaaat atatttataa ggcagaccct ggctgatcat actctgcaga ccatccgata
     1921 cctgcttttg atcatcccat ggttgtttgg tcaggaccag acaaaaagcc cagtgaagtt
     1981 tgaagagtga catgatctgc gtcataaaag ggtctccccc ggccgggtat tgcatgtgca
     2041 gcagggtgtt tagtcccgca gcctgcaaat catgctccag ctgtaaatca tcgatccctt
     2101 tataggcctg gtattctgct atatcaaaat catccgccgc agctgctgct tctccggtaa
     2161 aaaaaaccat acctctgtgt atagttcttt tcatgatggt catcttgtca gcccagcagc
     2221 aaaacaagaa agccaacgac ttgtagcgtt tggttttggt caaagtgttt ggctcagcaa
     2281 tcgtaataat cacgccttgt tcagacaaac gctgcgcagc gctgttgagc agcgttacag
     2341 gatctttaaa atgatggatc gcggaggaaa aaacaatgat gtcaaattgc ctgtcccgct
     2401 gcaaaaaggt tacggcatct tcctgaatca tttcaatgtc cccgatcttt tcttcacagc
     2461 tgcgttgtcg ggcaatttca agcatttttt ccgaaagatc aaccagcgta acttcagcct
     2521 gggggaccag ttttttcaat ataaaggctg attttccggc accgccgcac acatcaaccg
     2581 cccgcagttt ttgttcggac cgtgtcgcaa gcatctgcac aatggctgag aatatgacat
     2641 catattcagc atacgcatat ctgggttctt taaaatcccg ataataactc aaaatatgct
     2701 cattattgta tttttctata ttggccagta atatttcttc ctcgcttaca tcccgccatc
     2761 ccataatacg aaccctcgct tctaagtttc taatatgaat atattgccaa aagctctccg
     2821 tcttgcacct cagcacagg
//
LOCUS       DAOTHZ010000155         2737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_43894_length_2737_cov_4.444817, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000155 DAOTHZ010000000
VERSION     DAOTHZ010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43894_length_2737_cov_4.444817"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5549_10595"
     CDS             <1..410
                     /locus_tag="P5549_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13554.1"
                     /translation="RHGNGVFTWNSKVKYTGEFVKDKAEGYGTLSWSNGEQYTGQFGG
                     GEPNGLGTYSFANGDYYVGNMSGLTFEGVGTYYWATGARYVGEWEGDMLNGQGTYYWP
                     NGASQWGYWENDRYVGLYPEEAQSGDDEEEEDD"
     gene            complement(486..1508)
                     /locus_tag="P5549_10600"
     CDS             complement(486..1508)
                     /locus_tag="P5549_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRW13555.1"
                     /translation="MELKNLLAQTVQLQASDLHLKSGNPPIIRINGQITKLDLPALST
                     AETRFIARKILGNEKFEVFELQGELEGSYLQEDVGNFRISVYHEKGEVGMAFRVVSTI
                     IPNIGTLNLPPGLKNLLDLNSGMILVTGPTGSGKSTSQAAMVDYINGHRSCHIITLED
                     PIEFIHTNKKSIITQREIGTDSHSFPSALKAALRQDPDIIMIGEMRDLETISIALTAA
                     ETGHLVLATLHTTSAVQSISRIIDVFPSHQQRQAQVMLSNSFSGVISQRLIPGKDGQS
                     RYLASELMVATPAIRNLIREGKLHQIYSSIQTGARLGMQTLESSIQQLIAKGLIEDQA
                     TEVNGG"
     gene            complement(1558..2133)
                     /locus_tag="P5549_10605"
     CDS             complement(1558..2133)
                     /locus_tag="P5549_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRW13556.1"
                     /translation="MIDPSLKNNQGFTLIEGLIALAIFAIFIPLFFSLNSYIDRSFSI
                     ANRQSDLQFYSRLAKIQIEKEIQYVNSLEILSSAPSLENFSSGTEKAICVNADQELVR
                     LTKNAAGNLVETPLMQNVPDDLEMSLAFEKIIEENGGEDLYGNKLLGFVITCSSADET
                     VSLESSKLIKDTNVADELSIDGDTGSAIIYE"
     gene            complement(2139..2600)
                     /locus_tag="P5549_10610"
     CDS             complement(2139..2600)
                     /locus_tag="P5549_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRW13557.1"
                     /translation="MVHKVSWQDIIKSERGLTLLEIMVTLVIVCMFVVVITQFMGLSF
                     RDIFDKGNKMQAAAKAESLMEKITAIVENNNEAELDDTLQDLAGYVDDENDLLVYNAA
                     QPVQFFYEKPVYKDIDDSDSTPDVKGIDVDIVVFYKNGAASYRLKNFIYTG"
BASE COUNT          685 a          632 c          691 g          729 t
ORIGIN      
        1 gacgccatgg caacggtgtc tttacctgga acagcaaagt gaaatatacc ggcgaattcg
       61 tcaaggataa ggcagaagga tacggaacgc tgagctggtc caacggcgaa cagtataccg
      121 gtcagttcgg cggcggcgaa ccaaatgggc tgggaacata ttcatttgcc aacggtgatt
      181 attatgtcgg caacatgtcc ggtttgactt ttgaaggcgt cggcacctat tactgggcga
      241 ccggtgcgag atatgtgggc gaatgggaag gcgacatgct caacgggcag ggcacctatt
      301 actggcccaa cggcgcttcg caatggggat actgggaaaa tgaccgctac gtcggcttgt
      361 atccggaaga ggcgcaaagc ggcgacgatg aagaagaaga ggatgattaa cctgcaaacg
      421 caggcataaa aatatccggg gttctgcaga gaatcccgga ttttttgtgc ggagaaaccg
      481 cgtggttatc caccgtttac ttccgtcgcc tgatcttcaa ttaaaccctt ggcaatcagt
      541 tgctggatac tgctctccag cgtttgcata cccagtctgg ccccggtttg aatcgaggaa
      601 tagatctggt gcagtttgcc ttcccgaatc agattgcgga tggcgggtgt ggccaccatc
      661 agttccgagg ccagatatcg gctttggccg tccttgccgg ggatgagccg ctgggatata
      721 accccgctga agctgttgga gagcataacc tgcgcctggc gctgctgatg ggaggggaac
      781 acatcaatga tccggctgat ggactgcaca gcactggtgg tgtgcagggt cgccagcacc
      841 agatgaccgg tctccgccgc tgttaaagca atcgagatgg tttccagatc ccgcatctcc
      901 ccgatcatga tgatatccgg atcctgccgc agcgcggctt tcagggcgga agggaagctg
      961 tgggaatcag tcccgatttc acgctgggtg ataatgctct tcttgttggt atggataaac
     1021 tcaatcggat cttccagggt aataatgtga caggaacgat gtccattaat ataatcgacc
     1081 atggcagcct ggctggtgga tttacctgaa ccggtcggac cggtcaccag aatcatgccg
     1141 ctgtttaaat ccagcagatt cttcaggccg ggggggaggt tgagcgtgcc gatattgggg
     1201 atgatggtac tgaccacgcg aaaggccatg cccacctcac ctttttcatg gtaaacgctg
     1261 atgcgaaaat tgcccacatc ctcctgcaga taggaacctt ccagttcgcc ttgcagctca
     1321 aacacttcaa atttttcatt gcctaatatt ttacgggcga taaagcgggt ttcggcggtc
     1381 gataaagcgg gcaggtccag cttggtgatc tgtccattaa ttcggatgat gggaggattg
     1441 ccagacttca aatgcagatc ggaagcctgc agctgcaccg tctgggcgag cagattttta
     1501 agttccattt gtcagtcacg ctttctgcaa atattagccg ggaatagtaa gcaatcacta
     1561 ctcatagatg attgccgaac cggtatctcc atcgatactc agctcgtcag caacattggt
     1621 atctttgatt agtttgctgg actccaggct gacagtttca tcggcactgc tgcaggtaat
     1681 gacaaatccc aacagtttgt tgccgtataa atcttcacct ccattttctt ctattatttt
     1741 ttcaaaagca agtgacattt ctaaatcatc aggcacattc tgcatcaaag gcgtttcaac
     1801 tagattacca gctgcatttt tcgtcagccg taccagctct tgatcggcgt tgacgcaaat
     1861 tgctttttcc gttcctgaag agaaattttc cagcgaggga gcagaggaga gaatctcgag
     1921 ggagtttaca tattggattt ctttttcgat ttgtattttt gccagtctgg aatagaattg
     1981 caaatcagac tggcgattgg cgatggaaaa ggaacggtcg atgtaggaat tcagagaaaa
     2041 aaacaagggg ataaaaatgg cgaagatagc tagtgctatc aagccctcga ttagcgtaaa
     2101 tccctggttg ttttttaatg aaggatcgat cattttgttc atcctgtata aataaagttt
     2161 ttcaggcgat aggatgctgc tccgttttta taaaacacaa cgatgtcgac atctatgcct
     2221 tttacatccg gagttgagtc cgagtcgtca atatccttgt aaacaggttt ctcgtaaaaa
     2281 aattgaaccg gttgggcagc attatatacc agcaagtcgt tctcatcatc cacatatcca
     2341 gccagatcct ggagcgtgtc atccaactcg gcttcattat tgttttccac aatggcggtg
     2401 attttttcca tcagactttc ggctttcgcg gccgcctgca tcttgttgcc tttgtcaaaa
     2461 atatcccgga aactgagacc catgaattgt gtaatgacca caacaaacat acacacgatg
     2521 acgagggtca ccattatttc caataatgtc aaccctcgtt ccgatttgat tatatcttgc
     2581 caggacactt tatgcaccat tgggtttgat ccactttccg ctttgaatga ttctgttcaa
     2641 ggagggagtt acttcttctt cgtcatcgtt gttgtcgggc agattccatt ccagttgttc
     2701 aaagaagtcg gtactgaatg aatcgtcata agcaatg
//
LOCUS       DAOTHZ010000156         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_45689_length_2658_cov_5.086823, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000156 DAOTHZ010000000
VERSION     DAOTHZ010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45689_length_2658_cov_5.086823"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2009
                     /locus_tag="P5549_10615"
     CDS             <1..2009
                     /locus_tag="P5549_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HRW13558.1"
                     /translation="ELQNTYQCLPVFLTEKEIDRYYNGFCNETIWPLFHYFADKTKYD
                     IETWEAYRQVNEKFFKTVATVIGENDIIWVHDYQLMLLPRMIKEQFPDTQVGFFLHIP
                     FPSSEIFRLLIWREEILRGLLGADLIGFHTFEYVRHFLSSTRHLLGLENTMNRISYED
                     RYVQADAFPMGIDYQFFTQKHEDNSFQEEAREIIEKTKDMKIILSVDRLDYTKGIPER
                     LKAFGRFLTHYPEYREKVRFNLIVAPSRVEVDIYDDLRKEITELVSSINGNFGTFTWM
                     PVWFFFRSFSQESLIALYRHSDVLLVTPLRDGMNLVCKEYIAARTDYEGMVVISETAG
                     AASELGEAVIVNANDYNAIASGLKTALDMPREEKIARNKMMHRRLRRYNVDFWASEFL
                     HALQRTVSESKQTIMQRSIEKDSHLIENAYQNANRRVLFLDYDGTLVGFCPQPDQARP
                     DEELIQLLEVLVNDPRNTVVIISGRERYDLEEWFQNLNLYIIASHGLWLRYPGQEWIM
                     TTSLDNDWKDPIRHIMELYTDRIPGSLIEEKDYSLAWHYRQSDPDMIAVKLSEVRETL
                     RSIIQSETLGLQEGNKVLEVKDNRVNKGFGAAAFTQNQDYDFIFAVGDDYTDEDLFSS
                     LPQDAYTVKIGLGNTNAAYFLKSWQSMRLLLKKFADISNHLKKE"
     gene            2025..>2658
                     /locus_tag="P5549_10620"
     CDS             2025..>2658
                     /locus_tag="P5549_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase"
                     /protein_id="HRW13559.1"
                     /translation="MATTETVYEINDIPLDEYHISLNEALFHNANGYIGVRYNFEEGY
                     PQEYNFVRSQYINGFYDFTPMKQAENLYGLVNEKQTMLNIADTQAIKLFIDNEPFSMF
                     SGTVLSSRLWLDMDKGITVRKVVWRSPQGKELELTVTRMASFDQLSLFTIEYEVLPLN
                     FSGEVLIESGHNGEVSNYVDPDDPRMACEPMQHLTPLMCEIKEGVSYITSV"
BASE COUNT          763 a          538 c          636 g          721 t
ORIGIN      
        1 atgaactcca aaatacgtac cagtgcctgc cggtttttct caccgagaaa gaaattgacc
       61 gctattataa cgggttttgc aatgaaacga tctggccgtt atttcattat tttgcagata
      121 aaacaaaata tgacatcgag acctgggaag catatcgaca ggtaaatgag aaatttttca
      181 agaccgttgc gactgtgatt ggtgaaaacg atattatttg ggttcatgat tatcagttga
      241 tgttactgcc aagaatgatt aaagagcaat ttcccgatac ccaggtcgga ttttttcttc
      301 atattccttt tccttcgtct gaaatcttcc gtttgctcat ttggcgggag gagattctgc
      361 ggggactatt aggagcagac ttgatcggat tccacacctt cgaatatgtc cggcattttc
      421 taagcagcac gcggcacttg ctgggtctgg aaaatacgat gaaccggatt agctatgaag
      481 atcgctacgt tcaagcagat gcctttccca tggggattga ttaccagttc tttacccaga
      541 aacacgaaga caattctttt caggaagagg ccagggagat tattgaaaag acaaaagata
      601 tgaagatcat tttatcggta gaccgcctgg attataccaa aggcatcccg gaaaggctga
      661 aagcttttgg ccgcttcctg acccattatc cggaataccg ggaaaaagtc agatttaatt
      721 taatcgtcgc tccctcaaga gtagaggtcg atatctatga tgatttaaga aaagaaatta
      781 ctgaattggt cagttcgatc aacggaaatt ttggaacatt cacttggatg ccggtttggt
      841 tttttttccg ttcgttttcc caggaaagct taattgcgct ttaccggcat tcagacgtgt
      901 tattggtgac ccccctgcga gatggaatga atctggtgtg caaagaatat atcgccgccc
      961 ggactgatta tgaaggtatg gttgtaatta gtgaaacggc cggtgcggcc agcgaattag
     1021 gagaggcagt gattgtcaat gccaatgatt acaatgccat cgcgagcggg ctaaagacgg
     1081 cactggatat gccccgggaa gaaaaaatag ccagaaataa gatgatgcac cgccggctgc
     1141 gacgctacaa tgtagatttt tgggccagtg agttcttaca tgcgctgcag cgtaccgttt
     1201 cggaatcaaa gcagaccata atgcaaagaa gtattgagaa agacagtcat ctgattgaaa
     1261 atgcctatca aaatgccaat aggcgggttc tgtttttgga ttatgacggg actttggttg
     1321 gattttgccc ccagcctgat caggccaggc cggatgaaga actgatacag ttattggagg
     1381 tactggttaa cgatccccgc aataccgttg tgattatctc cggaagagaa aggtatgatt
     1441 tggaagaatg gtttcaaaat ctaaatctgt atatcatagc ctcgcatgga ttatggcttc
     1501 gttaccccgg gcaggagtgg attatgacca catccctgga taatgactgg aaagatccga
     1561 tccgtcatat tatggaatta tataccgacc ggataccggg atccttaatc gaagaaaaag
     1621 actattccct ggcctggcac tatcgacaaa gtgatcccga catgatcgcg gtcaaattaa
     1681 gtgaagtaag ggaaacactt cgctcgatca ttcaatctga aacccttggt ctccaggaag
     1741 gcaataaagt attggaagtt aaggacaacc gagtcaataa aggatttggt gcggccgcct
     1801 ttactcagaa tcaggactat gattttattt tcgcggtggg ggatgattat actgatgaag
     1861 atttattctc ttcccttccg caggatgcct acacggtcaa aataggcttg ggaaatacca
     1921 atgccgctta cttcctgaaa tcctggcagt caatgcggct tttgctaaaa aaatttgccg
     1981 atatcagtaa tcatttaaag aaagaataat tgaaaggaag aattatggct accactgaaa
     2041 cggtttatga aatcaatgac attccattgg acgagtacca tatatccttg aatgaagccc
     2101 tttttcacaa tgccaacggc tatatcgggg ttcgttacaa ctttgaagaa gggtatccgc
     2161 aagaatataa ttttgtacgc agccagtata ttaacggttt ttatgatttc acgcccatga
     2221 aacaggccga aaatctctat ggactggtca acgaaaaaca gaccatgtta aacatcgcgg
     2281 atacccaggc catcaagtta tttatcgata atgagccgtt cagtatgttc agcggcactg
     2341 tactgagcag taggctatgg ctggacatgg ataaagggat aaccgtccgg aaggttgtct
     2401 ggcgttcccc gcaaggcaaa gagctggagt taaccgttac ccgtatggct tcgtttgatc
     2461 agctgtcatt gtttacgatt gaatacgaag tgctgccgct taacttttcg ggggaggtcc
     2521 tgatcgagtc aggtcataac ggggaggttt caaactatgt agacccggat gatccccgca
     2581 tggcctgtga acccatgcag catttgacgc ctttgatgtg tgaaatcaag gaaggggttt
     2641 cctatattac ttcggtca
//
LOCUS       DAOTHZ010000157         2639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_46114_length_2639_cov_7.052245, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000157 DAOTHZ010000000
VERSION     DAOTHZ010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46114_length_2639_cov_7.052245"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2338
                     /locus_tag="P5549_10625"
     CDS             <1..2338
                     /locus_tag="P5549_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRW13560.1"
                     /translation="RAVEYAHLRGKKVYMTVNTLIDNHEFDGALEYLFQLYQRQVDAI
                     ILQDLGLASACRKVLPELRLHASTQMTIHNADGAAFIRDLGFKRIVLARELSADDIHL
                     IYQQVTNIELETFVHGAICYSYSGQCLFSSLVGGRSGNRGRCAQPCRLGYQLQSSSGR
                     YHVDAAQIGRYLLSPADLCLIEYLPELRDCGIQSLKIEGRMKRPEYVAVVTRAYREAL
                     DLMEDRGERPQPEAKDKMLKIFNRRFSSGYFIPAPADFLSTRRPNNRGVFVGRVLSQD
                     QRLQTRIKLGEKVQRGDGLAIWGNKGKNPIVHVKDILVDEKLQNEAQPGDIITVTLEE
                     RVSPDDRVFKTHDEQILQEAIKSIRDESDRAAVDAEVYLQSGEPLLLRLLDEQGNLAE
                     ARTRAAAQPARSQALDVAVLREKLGRMGNTPFVLRDLTITGNEALMLPFSELNEVRRQ
                     AVEQLMVQRLARVASPTVSHHKYEQRRSQLLPLSTARNHYLPMLNVAVSSFEQARAAL
                     QNGADQVLLGLEGLGSGRRPGKKQIRDWVAQHPADTDKIVPVLPRIHLPGDPYNGHDI
                     LQTGCRKIMVGNWGDLHWASTQDVQTMTDYSLNVFNAYSQQTLQTRGVVSVCLSPELN
                     FKQLQSWPDFAKVELLIHGRLQLMESQHCMLCTALGEEPGHCHAPCRRDEFSLQDEKQ
                     YVFPVATDADCRFYVFNSRTLCMMEDLPRFLKLGPQALRIEGRLMNEAELARTVRLYR
                     QALDQLAHDRTPDLTDLARQLPGAAQAFTKGHYYRGVL"
     gene            2339..>2639
                     /locus_tag="P5549_10630"
     CDS             2339..>2639
                     /locus_tag="P5549_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRW13561.1"
                     /translation="MDRETLTKLEFDKILAQLAALAYTAGARERIRQLMPLADLTLVN
                     LRLDETAEAMELLRFGEPGFLSSLQPVGQHLQKSRVAGILSPAELIDVYHLLRASR"
BASE COUNT          627 a          667 c          752 g          593 t
ORIGIN      
        1 ccgggcagtg gagtatgccc atttgcgcgg caaaaaagta tatatgacgg tcaataccct
       61 cattgacaac catgagtttg acggagccct ggagtatctt ttccaattat accagcgcca
      121 ggtggacgcg atcattcttc aggatctggg tttggcgtca gcctgccgca aagtattgcc
      181 ggaattgcga ttgcatgcca gtacccagat gaccattcac aatgccgatg gcgcagcctt
      241 catccgcgat ctcggtttta aacgcattgt tttggcccgc gaactgtccg cagacgatat
      301 ccatcttatt taccagcaag taaccaacat cgaactggaa acatttgtcc atggtgccat
      361 ctgttatagt tattcaggac aatgcctttt cagcagtctg gttgggggga gaagcggcaa
      421 ccgggggcgc tgcgcccagc cctgccgcct gggatatcag ttgcaaagca gttccgggag
      481 atatcatgtt gatgcagcgc agatcggacg ttatttgctc agtccggcgg atctctgtct
      541 aattgaatat ttgcctgaat tacgggactg cgggattcag tccctgaaaa ttgaaggacg
      601 gatgaaacgg ccggaatatg tggcggttgt cacccgggcc tatcgcgaag cgctggactt
      661 gatggaagac cggggggaac ggccccaacc ggaagccaag gacaaaatgt taaagatttt
      721 taaccgtcgc ttcagcagcg gctattttat tcccgcgccg gctgactttc tcagcacccg
      781 gcggcccaac aaccgcggtg tgtttgttgg tcgggtcttg tcccaggacc aacggctgca
      841 aacccgcatc aagctgggcg aaaaggtgca gcgcggggat ggactggcga tatggggcaa
      901 caaaggcaag aatccgattg tacatgtaaa agatatcctt gttgatgaga aactgcaaaa
      961 tgaagcccaa cccggggata tcatcaccgt aaccctggaa gaaagagtat caccggacga
     1021 tcgcgttttt aaaacccatg atgagcaaat tttgcaggaa gccataaaga gtattcggga
     1081 tgagtcggac cgagccgcgg tcgatgccga agtatatttg cagtcaggcg agccgttatt
     1141 gctccgatta ctggatgagc agggcaatct ggccgaagcc cggacgcggg cggcggccca
     1201 accggctcgc agccaggcgc tggatgtagc agtgctgcgg gaaaaactgg gtcgtatggg
     1261 caatactccc tttgtgctgc gtgatttgac catcaccggg aacgaagcgc tgatgctgcc
     1321 cttcagtgaa ctgaatgaag tgcgccggca ggcagtcgaa cagttgatgg tgcaaagact
     1381 ggcgagggtt gcttcgccaa cagtatccca ccataaatat gaacagcgcc gcagccagct
     1441 tttaccgtta tccactgctc gcaatcatta tttgccgatg ctgaacgtag ccgtatccag
     1501 ttttgagcaa gcccgcgcag ccttgcagaa cggcgccgat caggtactgc tgggactgga
     1561 agggttgggc agcggcaggc gaccggggaa aaaacaaatc cgggactggg ttgcacaaca
     1621 cccggcggat accgataaaa ttgttccggt cctgccgcgc atccatctgc cgggagatcc
     1681 atataatggt catgatatat tgcaaaccgg ctgccggaaa atcatggtcg gaaattgggg
     1741 agatcttcat tgggcgagca cccaggatgt ccagacgatg accgattaca gtttgaatgt
     1801 gtttaatgct tacagccagc aaacccttca aaccaggggg gttgtttcgg tctgtctttc
     1861 gccggaacta aacttcaaac agttgcaaag ctggccggat tttgctaaag tcgaattgct
     1921 gatccacgga cgattgcagt tgatggagtc gcagcattgc atgttgtgca cggcgctggg
     1981 agaagaaccc ggtcattgtc acgccccttg ccggcgggat gagtttagtc tgcaggacga
     2041 aaagcagtat gtatttccgg tggcaaccga tgcggactgt cgtttttatg tattcaattc
     2101 ccgcaccctg tgcatgatgg aagatttacc gcgttttctc aagctgggcc cgcaggcact
     2161 gcgcatcgaa ggccggctga tgaacgaggc ggagctggcc cgaaccgttc ggttatatcg
     2221 ccaggcgctg gatcaattgg cgcatgaccg tacgcctgat ttgactgatc tcgcccgaca
     2281 acttcccgga gcggctcagg cgtttaccaa aggtcattac tatcgaggag tattgtaaat
     2341 ggatcgggag acattaacca aactggaatt tgacaaaatc ctggcacaac tggccgcatt
     2401 ggcctatacc gccggtgccc gggagcggat tcggcaattg atgccgttgg ctgacctgac
     2461 actggtcaat ctgcgcctgg atgaaacagc ggaagccatg gagttgctga gattcggtga
     2521 acccggcttt ttaagctcgc tccaacccgt tgggcagcat ttgcaaaagt cacgggtagc
     2581 aggcattctg tcacccgccg agctgattga cgtctatcat ttgctgcgcg cctcgcgat
//
LOCUS       DAOTHZ010000158         2536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_48597_length_2536_cov_8.877872, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000158 DAOTHZ010000000
VERSION     DAOTHZ010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48597_length_2536_cov_8.877872"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /gene="mreC"
                     /locus_tag="P5549_10635"
     CDS             complement(<1..773)
                     /gene="mreC"
                     /locus_tag="P5549_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRW13562.1"
                     /translation="MLKLVGKKSFWALLVLAAVFLFLMYSSTGTRTEITAAEKVLRES
                     FAPLQNGLSGMKRGLAGVSINLQNKKDLARQLAQIEKQNQELRRENQQLQESAAEVIR
                     LRRLLDYKQSQQEDYDLTAARVIARQPNNWFRFITIDRGSSDGIKPGMAVISPDGLVG
                     RVSNCSHNASQVTLITDREMAVGAIVQNSRETQGIIEGMGEANSLRMINIPYYAKVGK
                     YERVVCSGLSQFYPKGILIGYIQSVEKEANGLLLTADIAP"
     gene            complement(789..1808)
                     /locus_tag="P5549_10640"
     CDS             complement(789..1808)
                     /locus_tag="P5549_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRW13563.1"
                     /translation="MFSKDMGIDLGTANTLVHLKGKGIVIWEPSVVAIQSHNGKVLAV
                     GEEAKKMIGRTPGNIIAIRPMKDGVIADFDITQAMLKYFINRAVEKRSLFMKPRIVIS
                     IPTGCTTVEERAVREAALSAGAKEAFLIEEPMAAAIGAGLPVHEPTGNMIVDIGGGTT
                     EVAVISLGGIVTAKSVRVAGDGMDSAIINHLKKTYNVLIGERSAELIKMNIGSALWDE
                     PEEFYEIRGRDLISGLPKTVEISSSEIQFALKETVEQIVEGIKECLEKTPPELAADIM
                     DRGIVLAGGGALLRGLDKLIGQETHMPVYVCEEPLLAVARGTGVVLENIEHLKKLLVT
                     VKKTF"
     gene            complement(1835..2518)
                     /gene="radC"
                     /locus_tag="P5549_10645"
     CDS             complement(1835..2518)
                     /gene="radC"
                     /locus_tag="P5549_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRW13564.1"
                     /translation="MDYQFGIKDMPPEMRPRERLSAHGEKNLSEQELLAIIIGNGTKN
                     VSALELAHRLLVHFQGLRNIREAELEELTQVKGIGPAKAVTIKAAIELGRRTALDVKI
                     KDFIHSPEDVSRLLMDEMRYLDREHLYGLYLDRKGGLLSKEIISIGSLSSSIVHPREV
                     FKSAVKCSASSVIVAHNHPSGDPTPSREDFDITRRLVEAGKIMGIELIDHLVIGHKSY
                     CSLKEKGAI"
BASE COUNT          573 a          734 c          532 g          697 t
ORIGIN      
        1 ggagcaatat ccgctgtcag caacaacccg ttggcttctt tttccactga ctgtatataa
       61 ccgatcagga tgcccttggg ataaaactgc gacaaccctg aacaaacgac tctttcatat
      121 ttgccgactt tggcataata gggaatgtta atcatacgca gcgaattggc ttcccccatg
      181 ccctcaataa tcccctgggt ctcacgactg ttctgcacga tcgctcccac cgccatctcc
      241 cgatcggtaa tcaacgttac ctgggaagca ttgtgactgc agttgctgac cctgccaacc
      301 agaccgtcag gactgattac cgccatcccc ggcttgattc cgtcgctgct gcctctgtca
      361 atggtgatga aacgaaacca gttgttgggc tgacgggcaa ttacccgggc agctgtcaga
      421 tcgtagtctt cctgttggct ttgtttgtag tccagtaatc tccttaagcg aatgacttcg
      481 gctgctgact cttgcaattg ctggttttcc cgccgcagct cctggttctg cttttcaatc
      541 tgtgccaact gacgggccaa atccttttta ttctgcaaat taatgctcac acctgccaag
      601 ccccttttca tcccgctcaa tccattttgc aggggggcaa agctttcccg cagcactttt
      661 tcagctgcgg ttatctcggt tcgggtgccg gtgctgctgt acatcagaaa taaaaaaacc
      721 gccgccagca ccagcaatgc ccaaaaactc ttttttccaa ccaactttaa caattatttc
      781 acccggtctt aaaaagtttt tttcacggtt accaacagtt ttttcagatg ctcaatgttt
      841 tccagcacga cgcctgttcc ccgggcaacc gccagcaacg gttcctcaca aacataaacc
      901 ggcatatggg tttcttgccc gatcagtttg tccagacctc gtaatagtgc tcctcctccg
      961 gccagcacga taccccggtc cataatatcc gcggccagct ccggcggagt tttttcgaga
     1021 cattccttaa tgccttccac gatttgctca acggtttcct tgagggcaaa ttgaatttca
     1081 ctagaggata tttccacggt tttaggcagg ccactaataa gatcacgacc gcgaatttcg
     1141 taaaattctt cgggttcatc ccacagcgct gagccgatat tcatcttgat caattccgca
     1201 ctgcgctccc caattaagac attgtaggtt tttttcagat ggttgatgat ggcactgtcc
     1261 ataccgtcac ccgccacccg gactgacttg gcagtgacga tcccgccaag agaaatcaca
     1321 gccacttctg tggttccgcc accgatatca acgatcatat tgccggttgg ttcatgtacc
     1381 ggcaaaccag ctccaatcgc tgctgccatc ggttcttcaa taagaaaggc ttctttggca
     1441 cctgccgaca gcgctgcttc cctcaccgct ctctcttcca ccgtagtaca accggtaggg
     1501 atgctgatca cgatgcgtgg tttcataaac aggcttcttt tttcgactgc ccggttgata
     1561 aaatacttca gcatggcctg ggttatgtca aaatcagcaa taaccccgtc tttcatcggt
     1621 cggatggcaa taatgttgcc gggcgttcgt ccgatcatct ttttggcttc ctcacccacc
     1681 gccaatacct tgccattatg actttgaatg gcaaccaccg aaggctccca tatgacaata
     1741 cctttgcctt taaggtgtac cagcgtattg gcagtgccca aatctattcc catatctttg
     1801 ctgaacataa ttcctgtatt gcctccttca atgcttagat tgcacccttt tccttcaggc
     1861 tgcagtagct cttgtgtcct atgaccaaat ggtctatcag ttcaattccc ataatcttgc
     1921 cggcctcaac caggcggcgg gtaatatcga aatcctcccg gctgggggtt ggatccccgc
     1981 tgggatgatt atgcgctaca atcaccgagg aagcactgca tttgacagct gatttaaaca
     2041 cttcccgggg atggacgatc gaacttgaca aactgccgat cgaaataatt tctttgctta
     2101 gcagaccgcc cttgcgatcc agatacagac catacagatg ctcgcggtcc aaataccgca
     2161 tttcatccat caacagccgg ctgacatctt cgggcgaatg gatgaaatcc ttgattttaa
     2221 catccaaagc cgtccgccgt cccagctcaa tcgctgcttt gatggtaacc gctttggcag
     2281 gaccgattcc ttttacctgt gtcaattcct ccagttcggc ttctctgata ttacgcaggc
     2341 cttgaaagtg aactaacagg cgatgcgcca attccaaggc actgacattt ttggttccgt
     2401 tgccgatgat aatggccagc aattcctgct cggaaaggtt cttctctcca tgagcactaa
     2461 gtctttcccg gggacgcatc tccgggggca tgtccttgat cccaaactgg taatccatca
     2521 ggcgttttcc ctccca
//
LOCUS       DAOTHZ010000159         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_48894_length_2525_cov_6.438462, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000159 DAOTHZ010000000
VERSION     DAOTHZ010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48894_length_2525_cov_6.438462"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /gene="pelF"
                     /locus_tag="P5549_10650"
     CDS             complement(<1..511)
                     /gene="pelF"
                     /locus_tag="P5549_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HRW13565.1"
                     /translation="MSAYYELYLASTYVFRIKDVKTLIRSLAMLKSRKIAFKMFILGP
                     LDEEEEYATECIELTTRLNLDDEVRFAGRVDVMEYLGLLDVVVLTSVSEGQPFVILEA
                     HCAGIPVVSTDVGSCSELLDGLTPEDRALGRSGLLTSVASAEETADALEYMARNPDET
                     YAMGQVGRKR"
     gene            complement(601..2187)
                     /locus_tag="P5549_10655"
     CDS             complement(601..2187)
                     /locus_tag="P5549_10655"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRW13566.1"
                     /translation="MTTITAHTEEDNMPKPQNGLKLKLGNLSNYEVEWSDQLNATPRG
                     GMMYLCHFLAKNNLFEDLVETCPLQYKSNNAPHKRSVLGTILCGILEGAWRYAHLSTI
                     KGDRISADALGLEKGFVSEDSVRRGMKKLTKDCWDKCQDWLSEAELTSVMPVLSEPYI
                     LDLDTSVKQIYGRQEGAEIGYNPSRCGRPSQSLHVGFIGTLRLLVYVDVQGGKNHAAC
                     HMSKGIWKSVDLIPKSMQPELVRGDIGYGNEGYMVECEQRDLQYLFKQKITSNGRKEI
                     VNVVTNDQTVWCAAPNTDYEYACSVLKLSGWSKERDIIILRRLVPSKKKGKKNGSKQM
                     LMSFMTDESGAVKKEWKYAILVHRTPNIPTESIPKLYQERADCENVLDELKNQWGLAG
                     FSTHDILRVKLMARLVAIVCNWWNVFTRMAEPDEHMEALTSRPELLHLIAQSISHAGK
                     QVIRFASYHENQPHIKAAVSRLHRCFAWIGSIAEQLTKSQILAIQMSIAFHRFLNGKL
                     LNVPDCARDGISQLCNSPPE"
BASE COUNT          647 a          635 c          531 g          712 t
ORIGIN      
        1 cccgcttgcg accgacctgc cccatagcgt aggtttcatc gggatttcta gccatatatt
       61 ccagcgcatc ggccgtttct tccgccgagg ccaccgacgt aagcagtcct gaacgcccaa
      121 gagcccgatc ttccggtgtc aatccatcca gcagttctga gcaggaaccg acatcggtgc
      181 tgacaacagg aatacccgca caatgcgcct ccagaataac aaagggctgg ccctcactga
      241 cactggtcaa taccaccaca tccagaaggc ctagatattc catgacatcg acacgtccgg
      301 caaagcgaac ctcgtcatcg agattcagcc gcgttgtcag ttcgatacac tctgtcgcgt
      361 attcctcctc ttcgtccagc ggtccgagga taaacatttt aaaggcaatt ttgcgggact
      421 tcagcatcgc cagggaccgg atcagcgttt tgacatcttt aatcctaaaa acgtaagtag
      481 aggctaaata cagctcataa tatgcggaca ttcgtcgcaa attctgtatg tcatgaatca
      541 gtgaacggct gtactcatat ttgtcaggat aaagttgatt gattgtattc agctgaaatt
      601 ttactctggc ggagaattgc acagttgaga gatgccatca cgtgcacaat cgggcacgtt
      661 cagtaacttt ccattcaaaa atctatgaaa agcaatggac atttgaatcg ccaatatttg
      721 cgattttgtc aactgctccg caatagaacc gatccaggca aaacaccggt gcagcctgct
      781 gacagcagct ttaatatggg gctgattttc atgataactt gcaaatctga tgacttgctt
      841 gcccgcatgg gatatagact gggctatgag gtgaagtaat tcagggcgcg aagtcagggc
      901 ttccatatgt tcatccggtt cagccattcg agtaaagaca ttccaccaat tgcaaacaat
      961 agccactagt cgcgccatca gcttgacacg caaaatatcg tgggtggaaa acccggcaag
     1021 cccccattgg tttttaagtt catcaagtac attctcacag tctgctcttt cctgataaag
     1081 tttaggaata gattccgtag gaatgtttgg tgtccggtgt acaagaatgg cgtatttcca
     1141 ttccttttta acagccccag attcgtctgt cataaatgac atgagcatct gtttggagcc
     1201 atttttcttt cccttttttt tcgagggtac taaacgacga agaatgatta tatctctttc
     1261 tttactccac ccggacagtt tgagtacaga gcatgcgtat tcgtaatccg tatttggtgc
     1321 ggcacaccag acggtctgat cgtttgtgac cacattaaca atctctttgc ggccattgct
     1381 ggttattttc tgtttaaaaa gatactgaag atcacgctgc tcgcactcaa ccatataccc
     1441 ctcgttgcca tagccaatat ctccgcgcac caactccggc tgcattgact tcggtattaa
     1501 atcaaccgat ttccatattc ctttggacat atggcaggcc gcatgatttt ttcctccctg
     1561 tacatcaacg tataccaaca aacgcagggt gcctataaac ccgacatgaa gagactgact
     1621 ggggcgtcca cacctgctgg ggttgtagcc gatttccgct ccctcctggc ggccatatat
     1681 ctgcttaaca gacgtatcca gatccaatat gtacggctcg ctaagcactg gcataactga
     1741 cgtcagttca gcttctgaca accagtcctg gcatttatcc cagcaatctt tggtcagttt
     1801 tttcatgccc cgacggacac tgtcttccga cacaaatcct ttctccaacc ccagagcatc
     1861 agcacttatt cggtcgcctt tgattgtcga taggtgtgca tagcgccatg caccctcgag
     1921 gattccgcac aaaatggttc ccagaacaga ccgtttgtga ggggcgttgt tgcttttgta
     1981 ttgaagcggg caagtttcca ccagatcttc gaacaaatta ttcttcgcca gaaaatggca
     2041 caaatacatc atcccaccac gaggcgttgc atttaactga tccgaccact caacctcata
     2101 attggataaa tttcccagtt ttagcttcaa tccgttttgt ggttttggca tgttatcttc
     2161 ttcagtgtga gctgtaattg ttgtcatcgt gtatcctcat tttgtgattg ttatgcggat
     2221 attatgatac acaattacgg ctcaccattt ggttttaaat cagaattgta ttacttccac
     2281 atgatcatga tgatacgcat caatagtgtc tccaacctac gcttttaggt tgatgctgta
     2341 tttgctgtaa atcagcctac atcagttggt gccttcaaag cactgcagaa cgaaaaaatg
     2401 gctggcaaag tcgtctttgt tgcttttgat tctgatcctg ttctcttgaa cggtgttgaa
     2461 aacaacgaag ttgaagccat catcgcacag aacccgtatg ccattggtta tgacggtgta
     2521 aaagc
//
LOCUS       DAOTHZ010000160         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_49353_length_2508_cov_7.421525, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000160 DAOTHZ010000000
VERSION     DAOTHZ010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49353_length_2508_cov_7.421525"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..1571)
                     /locus_tag="P5549_10660"
     CDS             complement(255..1571)
                     /locus_tag="P5549_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HRW13567.1"
                     /translation="MKKTWSVLLSVLLLFAFSSMAVASPINGVVKNKVKQVNKVNQEI
                     KEKYRTQKEKQVQVEDRVQDGNTIEKQVPKAARFKDMEKHWAKGTIELLAGLGLLYGY
                     PDGTFQPDQSVTQAECIELLMRLRGDVDEEDADEELDEEIDEDKEVPAWAQNAFKKAA
                     KKGIINVNRFHSAVQADRALVAVWVAKAMQLDPVDTSEMPYSDYLLISKEDLGYILAL
                     YKEGLMVGTPDGKFNPNSKITRAEMAAILQRILDEEGNDEEPVEDEVIAAELERTGQA
                     GIHEDKIYEEYQLVADDEVISLNEDNIRNLTLAKDEQDPVKLTPNADDTLWFNVQKES
                     ADYVLIAEDQDGNIYEAKLEWDAPEPVEYDFAGEIEVNYFQFELGDLDLTEVDKIYQI
                     DPDGDAAEITSNTTYLMFDTNNPDGKYIYLILNGTEWYTTTINFAD"
     gene            complement(1716..>2508)
                     /locus_tag="P5549_10665"
     CDS             complement(1716..>2508)
                     /locus_tag="P5549_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRW13568.1"
                     /translation="EVGRVILAKDPNTGGSLGCAISEAIEVSMRDDNCRYALGSVLNQ
                     VLLHQSIIGLESKIAMEKIEEYPDVVIGCAGGGSNLGGLMAAFMQDKLTGKGNARFIA
                     VEPASCPSMTRGKYAYDFCDTGRITPLARMYTLGSDFIPSPNHAGGLRYHGMSPILSK
                     LYHDGYMEAIAVEQNKVFEAATLFAKQEQILPAPESAHAIYGAVQEALRCKESGEAKT
                     ILFGLTGTGYFDMQAYTQFNEGTMTDYVPTDADLQRGFNGLPKVE"
BASE COUNT          571 a          644 c          602 g          691 t
ORIGIN      
        1 ctgaccgatc tgtcgtttgc ccatgatgcg cagaaagatg acggtcgtgg caaatagaat
       61 cagggtacgg ataatgacca gtaccatggc aatctcccgt ttcactcata ttgattatct
      121 tattatcctc tacgcccgga gaaaatatgt aaaaaggcgc gcgagagaat tgcagggctt
      181 aagccggaaa gaaaaattcg ataaaaaagg agcgcaatcg tcgctgcgac tgcgctcccc
      241 ggggaagaga ttcgctaatc tgcaaaattt atggtggttg tgtaccactc ggttccgttc
      301 agaattaaat agatatactt gccatccgga ttgttggtat caaacattaa ataggtcgtg
      361 ttgctggtta tttccgctgc atctccgtca ggatcgatct gatagatttt atcaacttca
      421 gtcaggtcca gatctcccag ttcaaactga aaataattga cctcgatttc accggcaaaa
      481 tcatactcaa ccggttcagg ggcatcccat tccagtttgg cttcatatat atttccgtct
      541 tggtcttccg caatcagaac atagtcggca ctctcttttt gtacgttgaa ccagagggtg
      601 tcgtcagcat tgggggtcag cttgaccggg tcctgctcat ccttggcaag cgtgagattg
      661 cggatgttgt cttcattcag ggaaatcact tcatcgtctg ccaccagttg gtattcttcg
      721 tagattttat cttcatggat gcctgcctgt ccggtcctct ccaactcggc agcgattacc
      781 tcatcttcca caggttcttc atcgtttcct tcttcatcca aaatgcgctg caggatggca
      841 gccatttctg cccgggtaat cttgctgttg gggttaaact tgccatccgg cgtaccgacc
      901 atgagtcctt ccttatatag ggccaggatg tagccgaggt cctctttgga aatcagcaga
      961 taatccgaat agggcatttc actggtatct accggatcaa gctgcatggc tttggcgacc
     1021 cataccgcga ctaaagcacg atcggcctgc acagcggaat ggaaccggtt aacattgatg
     1081 atcccttttt tggcagcttt cttaaaagcg ttttgcgccc aggcgggaac ttccttgtcc
     1141 tcatcaattt cttcatccag ttcttcgtct gcatcctcct catccacatc tccgcgcaga
     1201 cgcatgagca gttcaatgca ttcggcctgc gtgacggact gatcaggctg aaacgtgcca
     1261 tcgggataac cgtataacaa acccagcccc gccagcaatt cgatggttcc tttcgcccag
     1321 tgtttttcca tgtctttgaa cctggcagcc ttgggcactt gcttttctat tgtattccca
     1381 tcttgtaccc ggtcttcaac ctgcacttgc ttttcttttt gggtccgata tttttctttg
     1441 atttcctgat tgactttatt gacttgcttg actttgtttt tgaccacacc attgatcgga
     1501 ctcgcaactg ccatactgct gaaggcaaat aaaagcaata ctgacagtaa aacagaccat
     1561 gtcttcttca tcatcattcc tcctttacta tattattcgc aggccggttt aataaattgg
     1621 ggtcagcaga gaataaaaac aaacaggggg acgttctttt ggttggcctg tgcaaacaag
     1681 ccagcctaaa gaacgtcccc ctgttggaaa gttggttatt ctactttggg caatccgttg
     1741 aagccgcgct gcaagtcagc atcggtgggg acataatcag tcatcgtgcc ttcgttgaac
     1801 tgggtgtagg cctgcatatc gaaatagccg gtgccggtca ggccaaacag gatggttttg
     1861 gcttcaccgg attccttgca gcgcaaagcc tcctgcaccg ctccgtagat ggcatgcgct
     1921 gattccgggg caggcagaat ctgttcttgt ttggcaaaca gggtcgcggc ttcaaagact
     1981 ttgttttgct cgacggcgat tgcttccatg tagccgtcat gatacagctt ggacaggatg
     2041 ggtgacatgc cgtgatagcg cagtccgccg gcatgattgg gagaaggaat aaaatcgctg
     2101 cccagggtgt acattctggc cagcggcgtg attcttcccg tgtcacagaa gtcatacgca
     2161 tacttgccgc gggtcatgga cggacaggaa gccggttcta cagcgataaa acgggcattg
     2221 cctttgccgg tcagtttgtc ctgcatgaac gctgccatca ggccgcccag gttggagcct
     2281 ccgccggcgc agccgatgac cacatccggg tattcttcga ttttttccat ggcgatcttg
     2341 ctttccagac cgatgatcga ctgatgaagc aggacctgat tcaaaaccga acccagggca
     2401 tagcggcagt tgtcgtcgcg catggatact tcaatcgctt cagaaattgc acaaccgaga
     2461 ctgcccccgg tgttgggatc tttagccaga atgacgcggc ctacttca
//
LOCUS       DAOTHZ010000161         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_50349_length_2470_cov_4.992961, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000161 DAOTHZ010000000
VERSION     DAOTHZ010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50349_length_2470_cov_4.992961"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="P5549_10670"
     CDS             complement(<1..421)
                     /locus_tag="P5549_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516474.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HRW13569.1"
                     /translation="MKITVAGTGYVGLSNAVLLAQHNEVTAIDIIKEKVEMINKRVSP
                     IVDHEIEEFFAGKELNLKATIDNYEAYNEAEFVIVATPTDYDPDLNYFNTRTVEGVIA
                     NVLAINPDAVIVIKSTVPVGYTNKVKDMFATDNIIFSP"
     gene            complement(519..1427)
                     /locus_tag="P5549_10675"
     CDS             complement(519..1427)
                     /locus_tag="P5549_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13570.1"
                     /translation="MKLAILTVYLVTPDTSRLIDLQLEMIGRHTREYTIYAAINRLQP
                     EFKAKLNGHPGVKICSIPETDQRGSAELTYYLEHMAAQAVSEGATHLVTLHVDSFPVA
                     DDWSETIAGRLSAQCVLAAPSEHGSPRMPSAGLFFTREFYLQYRPVFRIDDAEQESEG
                     MQQFRNKYNVYSWDSGMGYIYRADEAGLDWYPLKRLEKRGGAAIYEGLLFHLEGGFRY
                     GSPKKQDPSLPDKIIRQCLYAVKYALMPILPKSIIYNRNSLLRKVLALMNRKMIIDSL
                     YGEEKKDLLADPEKFLQSLRDSWGRS"
     gene            complement(1424..>2470)
                     /locus_tag="P5549_10680"
     CDS             complement(1424..>2470)
                     /locus_tag="P5549_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13571.1"
                     /translation="VLAVCTDGEMLARCGQLDAVIEATGTIMDGGRFAVSALKAKKHL
                     ILMNSEIDLIFGPYFQQLAAENGVVCTSADGDQYGVLKHMMDDINRWGFKTVMAGNVK
                     GFLNRYAHPDMMIEEARKRNLDVRACTSYTDGTKLNIEMALIANAEGLQTICPGMQGP
                     RLEHISQVKGAYDFPSLGDKQQPRVDYILGAEPGGGVFVIGYCDHPYQQDMLQYYKMG
                     AGPFYLFYRHYHLCHIEALSTVVQAVRQKKALMQPDHGFVTNVYAYAKRDLAAGDMLD
                     GIGGFSCYGLIENREDNLIAPGLPICLTDQIRLKRSVKKDEKILWDDVEINNERLDFS
                     LFRLASLAGGNTTL"
BASE COUNT          579 a          654 c          594 g          643 t
ORIGIN      
        1 ccggggaaaa aatgatatta tcggtcgcaa acatatcctt taccttattg gtataaccca
       61 ccggcaccgt tgatttgatg acaatgacgg catccggatt gatggccagt acattggcga
      121 tgaccccttc caccgtccgg gtgttgaaat aattcagatc cggatcgtag tcagtcgggg
      181 tagccacgat cacgaattcc gcctcattat aggcctcata gttatcgatg gttgccttta
      241 aattcagttc tttcccagcg aaaaattcct ctatttcatg gtcaacgatt ggagagaccc
      301 gcttattgat catctcgact ttttccttga tgatatcgat ggccgtcact tcattatgct
      361 gcgccagcaa aaccgcattg gacagcccca catagccagt ccccgccacc gttattttca
      421 tcggttcctc tcctcattgc cattttgcag taattatatc ataaagaagt gcagacaaga
      481 tgacggttct tatgtctcct caccaaaaag gtgacatttt aggaacgtcc ccaactgtca
      541 cgcagggact gcagaaattt ttccgggtcg gccagcaaat cttttttctc ttcaccatac
      601 aaagagtcga tgatcatttt gcggttcatg agggccagca cctttcttag caggctgttg
      661 cggttataaa taatactttt gggcaggatg ggcatgagag catatttgac cgcatacagg
      721 cactggcgaa ttattttgtc tggcaggctg ggatcttgct ttttcggact gccatagcgg
      781 aatcccccct ccagatggaa gagcagaccc tcataaatgg cggccccgcc tcttttctcc
      841 aggcgtttta aaggatacca atccaatccg gcttcatctg cccggtagat atagcccatg
      901 cctgaatccc aactgtagac attatattta tttctaaatt gctgcattcc ttctgactcc
      961 tgctccgcgt catcgattcg gaaaactggt cggtactgca gatagaattc ccgggtgaaa
     1021 aacagcccgg cggatggcat gcgcggactt ccatgttcgc tgggggcagc caggacacat
     1081 tgagcagaaa gccggccggc gatggtttcg ctccaatcat ccgccaccgg gaaagagtcc
     1141 acatgcaggg tgaccagatg agtagcccct tcggacacgg cctgcgctgc catatgctcc
     1201 agataatagg tcagctctgc ggaaccacgc tggtcagttt ccgggatgct gcatattttg
     1261 actccgggat gtccgtttaa tttggcttta aattccggct gcagacggtt gatcgctgcg
     1321 tagatggtat attcacgggt atgacgcccg atcatttcca attgcaggtc gatcaggcgg
     1381 ctggtgtcgg gggttaccag atacacggtc aggatggcta atttcatagt gtggtgtttc
     1441 ctcctgccag gctggccaga cgaaaaagcg aaaaatcaag gcgctcgtta ttgatttcca
     1501 catcgtccca caggatcttc tcgtcttttt taacactgcg tttgagccgg atctggtccg
     1561 tcaggcatat aggaaggccg ggggctatca gattgtcctc ccggttttca atcaaaccgt
     1621 aacagctgaa accaccgatc ccgtcaagca tatcgccggc agccagatcc cgtttggcat
     1681 aggcataaac attggtcaca aaaccatgat cgggctgcat caaagctttt ttctgccgga
     1741 cggcttgaac cactgtggac agggcttcga tatggcagag gtggtaatga cgatagaata
     1801 aatagaaagg cccggcgccc attttataat attgcagcat atcctgctgg taaggatggt
     1861 cgcagtatcc gatgacaaaa actccgcccc cgggttctgc gcccagaata taatccaccc
     1921 ggggctgctg tttgtccccg agagaaggga aatcataagc ccccttcacc tggcttatat
     1981 gttccaaccg gggcccctgc ataccgggac atatcgtctg caaaccttct gcgttagcga
     2041 tcagggccat ttcgatattc agcttggtcc cgtcagtata agaagtgcag gcgcgcacat
     2101 ccaggttacg cttgcgggct tcttcgatca tcatatccgg atgggcataa cggttcaaaa
     2161 aaccttttac gttgcccgcc atgactgttt tgaatcccca tctgttgatg tcgtccatca
     2221 tatgtttgag gacaccgtat tgatcaccgt ctgcgctggt gcagacgact ccattctctg
     2281 ccgccagttg ctggaagtaa ggaccaaaaa tcaaatcgat ttcagaattc atcaggatca
     2341 gatgcttctt cgctttcaat gcgcttacgg cgaaacggcc tccgtccatg atggttccgg
     2401 tcgcttcaat gactgcatcc agctgcccgc agcgggccag catttctccg tctgtgcaaa
     2461 cggccaggac
//
LOCUS       DAOTHZ010000162         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_62141_length_2115_cov_6.170388, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000162 DAOTHZ010000000
VERSION     DAOTHZ010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62141_length_2115_cov_6.170388"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="P5549_10685"
     CDS             <1..780
                     /locus_tag="P5549_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRW13572.1"
                     /translation="PSVLKEEDLEDLFALLGQYRITSTAKEITVEAGRPDTLPVPKLK
                     ILKALGTNRICINPQTMQEATLQLIGRRHGRADVLDAVERVREVGFPVVNMDIIIGLP
                     GEGRAEYQNTLTQILDLKPENITVHSLAVKKGSRLAALQGRQSMQEQAGEVQFGIDSA
                     SRQLQEKGYLPYYLYRQKYMKANVENIGYSLPGHFCRYNIQIMEERQTVIGLGGGAGS
                     KFVNPGDWSLVNLHSPKDPVSYGQHLPELLARKVDKLRALN"
     gene            818..2080
                     /gene="hisS"
                     /locus_tag="P5549_10690"
     CDS             818..2080
                     /gene="hisS"
                     /locus_tag="P5549_10690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRW13573.1"
                     /translation="MQVKAPRGTYDIMPDEIHKWHRMEQIIRCTAETFGYMEIRTPIF
                     EHTELFERGVGDTTDIVSKEMYTFQDRSHRSLTLRPENTASCVRAFIEHSVAGGVLPV
                     KWYYMGPMFRYDRPQAGRYRQFHQFGVESFGSSNPAMDAEVISLVLEILANLGLHEYE
                     LHLNSVGCPQCREEYRQKLIDHIRPVKDRLCGDCQTRFEKNPLRVLDCKVSSCQEAIV
                     GFPNLYDHLCPDCSEHYRRVRTILSEAGISYIHDNRLVRGLDYYTNTAFEVMIPLIGA
                     QSAVAGGGRYNGLVRECGGTDMPGVGFAMGLERLLLALDNLPKEKYEAKTIEVFLVLL
                     DDQYETEAAGILAALRRAGIKSDRDYNQRSAKAQMKYADKMGARLVLLLGEDEMQRGV
                     VTIRDMQTRQQSEVAREVLIAAVKAILE"
BASE COUNT          539 a          499 c          607 g          470 t
ORIGIN      
        1 cccagcgttc tcaaggaaga ggatctggag gatctgtttg cgctgttggg gcaatatcgt
       61 atcacctcca ctgccaagga aatcaccgtg gaagccggaa ggcctgatac gctgcccgtt
      121 cccaaattaa aaattttaaa agccctgggc accaaccgca tctgcatcaa tccccagacc
      181 atgcaggagg ccaccttgca attgatcggc cgccgtcatg gccgggctga tgttttggat
      241 gccgttgagc gggtccggga ggtgggattt cccgttgtga atatggatat aatcatcggg
      301 ctgccgggag aggggagggc ggagtatcaa aatacgctga cccagatatt ggacctcaaa
      361 ccggaaaaca tcactgtcca tagtctggcc gttaaaaaag gctcccggct ggcggcgctg
      421 cagggcaggc aatccatgca ggagcaggcc ggggaagtgc agttcggcat tgatagtgca
      481 agccggcagc tgcaggaaaa aggttatctt ccctattatt tataccgtca gaaatatatg
      541 aaagccaacg ttgagaatat cggctattca ttaccggggc atttttgccg ctataacatc
      601 caaatcatgg aggagcgcca gaccgtgatc ggtttgggag gaggtgccgg cagtaaattt
      661 gtcaaccctg gggactggag tctggtcaat ctgcattctc ccaaagatcc tgtcagctat
      721 ggccagcatc tgccggaact gctggcccgc aaagttgaca agctcagggc cttaaattag
      781 aattatccaa agaatagttg cgttggagga aggttgaatg caggtgaaag ccccccgggg
      841 cacatacgat attatgccgg atgaaatcca caaatggcat cgcatggaac aaatcatcag
      901 atgtacggca gaaacattcg gttacatgga aatcaggacc cccatcttcg agcacacgga
      961 attgtttgaa cggggcgttg gagatacaac cgacattgtc agcaaggaaa tgtatacctt
     1021 tcaggatcgt tcccaccgga gcctgacctt gaggccggaa aacactgcct cctgtgtgcg
     1081 ggcttttatt gagcacagcg ttgccggagg cgttttgccg gtaaaatggt attacatggg
     1141 tccgatgttt cgctacgacc ggcctcaggc cggaagatac cggcagttcc atcagttcgg
     1201 ggtagagtcc tttggcagca gcaatccggc catggatgcc gaagtcatca gcctggtgct
     1261 ggaaattctg gccaacctgg gtttgcatga atacgaactg catttaaatt cggtgggatg
     1321 cccgcaatgt cgcgaagaat accggcaaaa actaattgac catatccgcc cggtcaaaga
     1381 cagactgtgc ggcgattgcc agacccgttt cgaaaaaaac ccgttgcggg tgctggattg
     1441 caaggtctcc tcatgccagg aagccattgt cggttttccg aatttatatg atcatctgtg
     1501 tccggactgc agcgagcatt atcgccgggt gcgcaccatc ctgagcgaag ccgggataag
     1561 ttatatccat gacaatcgtt tggtgcgcgg tctcgattat tacaccaata ctgcctttga
     1621 agtcatgatt cccctcatcg gtgcgcagag cgcagtcgcc ggcggaggcc gctacaacgg
     1681 gctggtcagg gagtgtggag ggaccgatat gcctggcgta gggtttgcca tgggactgga
     1741 aagattgctg ctggcgctgg acaacttgcc caaagagaag tacgaagcaa aaacaatcga
     1801 ggtatttctg gtcttgctgg atgatcaata tgaaactgag gcggcgggga ttcttgctgc
     1861 tttgcgccgg gccggcatta aatctgatcg ggattacaat cagcgcagcg ccaaagcgca
     1921 gatgaaatat gcagataaaa tgggagcccg tctggttctg ttgctgggcg aggatgaaat
     1981 gcaaagaggt gtggtaacca tacgcgacat gcagacccgt cagcagagtg aagttgccag
     2041 ggaagtattg attgccgccg ttaaagcaat tcttgaatag attgggagga tattgccaga
     2101 atgaaaagga cgcat
//
LOCUS       DAOTHZ010000163         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_62349_length_2110_cov_6.970803, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000163 DAOTHZ010000000
VERSION     DAOTHZ010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62349_length_2110_cov_6.970803"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..138)
                     /locus_tag="P5549_10695"
     tRNA            complement(60..138)
                     /locus_tag="P5549_10695"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Met,seq:cat)
     gene            complement(434..1618)
                     /locus_tag="P5549_10700"
     CDS             complement(434..1618)
                     /locus_tag="P5549_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRW13574.1"
                     /translation="MKKSGGVFTLSAGAAGLGIVIGAISAAFLYLIDWGQHAVWESGW
                     IQFKYAPLLICSLGGILIGFCQRYLGDHPKNLHGAVEEIRKTGRLEYAHLPNGLISAF
                     ISLIFGASLGPEAAVVNLLGGLSTWASDKLKKLSSWLRIPESTPDRTLVHRLARRWPN
                     VLAISIAVVIFIIGIKDLYGGGVLKLNEPFAWIDLLWSIPMALLGATGGALFLGLGRW
                     MKQRLRFLKQKPVLRGVLSGCVLGLIACFLPMMLFSGQLYLQQVYEQAAILGFWMLLW
                     IALARLFLINLLLESGWKGGQFFPLMFASVAFGLSIKNLFPFVPDSAAIFGTMAAMIT
                     IVLPKPIVVLIVMAIFFPPQYLGISAVGVGVVMFGDWLWKRIKERVVNKTNRFTNHSL
                     VD"
     gene            1798..>2110
                     /locus_tag="P5549_10705"
     CDS             1798..>2110
                     /locus_tag="P5549_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRW13575.1"
                     /translation="MARLNSLTERGKETQRLLGEILLKLITEKGYDKITVKDITDKAN
                     IDRTTFYLHFKDKDDLFLKSQQQIIDELIEYGQKIDQPYPRATLVFEHMAQHSEAYRV
                     LL"
BASE COUNT          630 a          524 c          448 g          508 t
ORIGIN      
        1 accgctgtgc gccgggctaa tcaaaaacca gagcccgacg gactctggtt tactcttagt
       61 agcggggagt ggattcgaac cactgacctc cggattatga gtcaaaatcc tgaatccgta
      121 ccgtcggcac atcccgcccg aagggtaggg atctgtgcgg caggctctgc aaaccctggc
      181 accgcccgcc agggcaggtg tcagccgacc tgacgaactc tggtccgcac ttgccgggcg
      241 ctcagcggtc ccacaagggg atgacagtac caatcgattt tcgtatagta tctatttttc
      301 cgtgggttcg tcaagtagtt tttccggttc tcgaaaacag ttaacgagta aggtaatgca
      361 gacatgcacc ctaaccgcaa atgtcttatg ggcagtaatt tggagtaacc catttttttg
      421 tttattcaat aaatcaatcc accagtgaat ggttggtaaa tctatttgtc ttgttaacta
      481 ccctctcttt tatccgtttc cacaaccagt caccaaacat aacaacgcca actcctactg
      541 cagatatacc cagatattgc ggtgggaaaa atattgccat tacgatcaat acgacgattg
      601 gttttggaag cacaatcgtt atcatggctg ccatcgtgcc aaatattgcc gccgaatcag
      661 ggacaaatgg gaataaattt tttattgaaa gcccgaaggc tacactggcg aacattaatg
      721 ggaaaaactg accacctttc catccgcttt ccaaaagtag attgatcaga aataatctcg
      781 ccagagcgat ccacaacagc atccaaaatc ccaagatggc tgcttgttca tagacttgct
      841 gcaagtagag ctggccagag aaaagcatca ttggaaggaa gcaggcaatc aaccctagaa
      901 cacatccact caagacccct cgcaaaactg gcttttgctt aagaaacctc aacctttgtt
      961 tcatccatct tcctaggcca agaaaaagag cgcctcctgt ggcaccaagc agagccattg
     1021 gtatgctcca cagcaggtca atccacgcaa agggttcgtt caatttcaaa acaccgccac
     1081 catataggtc cttgatacca attatgaaaa tgacaacagc tatacttatt gccagaacat
     1141 ttggccatcg ccgtgcaagg cggtgaacaa gagttctatc tggtgtagat tcaggaatcc
     1201 tcagccaact gctcagcttt ttcagtttat cggatgccca ggtggaaagt ccaccaagca
     1261 aatttacaac agcagcttct ggccccaggc ttgcaccaaa aatgagggaa atgaacgccg
     1321 agatcaaacc gttgggcagg tgagcgtact ccaaccgccc ggttttccgt atttcttcaa
     1381 cggctccgtg cagattttta ggatggtcac ccaggtagcg ttgacaaaac ccaatgagaa
     1441 ttcctcccaa actgcaaata agcaagggcg catatttaaa ttgaatccaa cccgattccc
     1501 ataccgcatg ttgtccccaa tctatcagat acaaaaaagc agcggatata gctccaatta
     1561 caattcccag ccctgctgct ccagctgata gagtaaaaac accgccactc tttttcaatg
     1621 ttgcgcactc cgatcgaggt ttaagtcata aacaggttat aatatcaaca cagtgttgat
     1681 atctacacga tgtcgataat aatgcggata ccggcccttg tcaaggtgat cggtgtgaga
     1741 aacaaaatta caaaaaaagt aattcacgaa tggttgtctt cagagtagga gtgaatcatg
     1801 gcgcgactta atagtctgac cgaaagagga aaagagactc aacgtctgct gggtgagatt
     1861 ctcttgaaat taattaccga gaaaggttat gacaagatca cagtcaagga tatcacggat
     1921 aaggccaata tcgaccgcac cacattctac ctgcacttca aagataaaga cgacctattt
     1981 cttaaaagtc aacagcaaat aatagatgag ctcattgaat acggccaaaa aattgaccag
     2041 ccttatcccc gagccaccct ggtttttgaa catatggcgc agcattctga ggcatatcgg
     2101 gtgttgttga
//
LOCUS       DAOTHZ010000164         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_63626_length_2078_cov_5.775087, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000164 DAOTHZ010000000
VERSION     DAOTHZ010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63626_length_2078_cov_5.775087"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2078
                     /gene="carB"
                     /locus_tag="P5549_10710"
     CDS             <1..>2078
                     /gene="carB"
                     /locus_tag="P5549_10710"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRW13576.1"
                     /translation="IKRIIIKERPDSVLSTLGGQTGLTLSMQLAKEGFLQKHGVKLLG
                     ANPATIDKAEDRQMFKDTMQSIGEPVIPSLVVTDVPAALAFAEEIGYPVIVRPAFTLG
                     GTGGGIASNQKELQEIAANGLRLSPITQVLIEQSVAGWKEIEFEVMRDSAGNVITVCS
                     MENFDPVGVHTGDSIVIAPAVTLSDKEYQMLRSAALNIISALEVEGGCNCQFALHPTS
                     FEYAVIEVNPRVSRSSALASKATGYPIAKVAAKIAIGYTLDEIKNAVTGKTYACFEPA
                     LDYVVVKLPKWPFDKFVYAQRTLGTQMKATGEVMAIGTSFEQCIMKAVRGAEISLDSL
                     NLPKLKELSDLQIEQLLTECNDERLFVIFAALQRGVSCAAIHDTTKIDLWFLHKLTNL
                     VMLEKELAKGNLSPELYLTAKNMGYPDKVITRLSGCEIKNPRQASYKMVDTCAAEFEA
                     ETPYFYSTYDEVNEAEQFIQARSSGKKTVIVFGSGPIRIGQGIEFDYASVHCVWALKK
                     AGYEVVIVNNNPETVSTDFDTADRLYFEPLSNEDVMNVIHTEQPYGVVVAFGGQTAIK
                     LTRFLEAQGVLILGTPADSIDAAEDRERFDDLLGKLSIKRPQGHTVMNTQEALQAANK
                     LEYPVLMRPSYVLGGQNMIIAFSDDDIREYMDIILAYNIENPVLIDKYLMGIEIEVDA
                     ICDGEDILIP"
BASE COUNT          530 a          496 c          553 g          499 t
ORIGIN      
        1 ccatcaagcg gatcatcatc aaggaacgcc ctgacagcgt tttatcaacc ctgggcggac
       61 aaaccggcct gaccctgtcc atgcagctgg ccaaagaagg atttttgcag aaacacgggg
      121 tcaaattatt gggggccaat ccggcaacca tcgacaaggc cgaggaccgg caaatgttta
      181 aggacaccat gcaatcgatt ggtgagccgg tcattccatc cctggtcgtg actgatgttc
      241 ctgctgccct ggcttttgcg gaggagattg gctatccggt tatcgttcgt cccgctttta
      301 cactgggggg aacgggcgga ggaattgctt ccaaccagaa ggaattgcag gaaattgccg
      361 ccaacggcct taggctttct cctattaccc aggtgctgat cgaacaaagt gtggctgggt
      421 ggaaggaaat cgaatttgaa gtaatgcggg acagtgccgg caatgtcatc actgtctgca
      481 gcatggagaa tttcgatccg gtgggcgtgc ataccgggga cagcattgtt attgcgccgg
      541 ccgtgaccct ttcggacaaa gaatatcaga tgctgcgttc cgccgcctta aatattatca
      601 gtgccctgga agtggaaggg gggtgcaatt gtcagtttgc gctgcatccc accagttttg
      661 agtatgccgt gattgaggtc aacccccgag tatcccgttc atccgcgctg gcttccaagg
      721 caaccgggta tccgatcgcc aaggtggcag ccaagatcgc cattggctat accctggatg
      781 agatcaaaaa cgctgtgacc ggaaaaacct atgcctgttt tgaaccggcg cttgattatg
      841 tagttgtcaa actgcccaaa tggccctttg acaaattcgt ttacgcccag cgtacgctgg
      901 gcacgcagat gaaggccacc ggagaagtaa tggcgattgg aaccagcttt gaacagtgca
      961 tcatgaaagc ggtgcgggga gcagagattt ccctggacag tctcaatctg cccaagctga
     1021 aagaactgtc cgatctacaa atagaacaac tgctcacaga atgtaatgat gaacgccttt
     1081 ttgtaatctt tgcagccctg caaagaggtg tttcctgtgc agccattcat gataccacta
     1141 aaattgatct atggttcctg cacaagctga ccaatctggt aatgctggaa aaagaactgg
     1201 ccaaaggcaa tttatcccca gaattatatc ttaccgcgaa aaatatggga tatcccgata
     1261 aagtcatcac ccgtctttcc ggttgtgaaa tcaaaaatcc gcggcaggct tcctacaaga
     1321 tggtggatac ctgcgcggct gaatttgagg cggagacgcc atatttttac tccacctatg
     1381 atgaggtcaa tgaagcggag cagtttatcc aagccaggag cagcggcaaa aaaaccgtga
     1441 ttgtattcgg ctccggtccg atccggatcg ggcaggggat cgagtttgac tacgcttccg
     1501 tgcattgtgt gtgggcgctg aaaaaagccg gttatgaagt ggtcatcgtc aacaacaacc
     1561 ccgaaaccgt ttcaaccgac ttcgataccg ctgatcggtt gtattttgaa cctttgagca
     1621 atgaagatgt gatgaacgtg atccatacgg agcagcctta tggagtggtg gtcgcttttg
     1681 gaggtcagac cgccatcaag ctgaccaggt tcctggaggc gcagggtgtt ctgatattag
     1741 gtacacctgc cgatagtatc gatgctgccg aggatcggga gcgttttgac gatctgctgg
     1801 ggaaattatc cattaaacgt ccccagggcc atacggtgat gaatacccag gaagccctgc
     1861 aagctgccaa caaactggaa tatccggttt taatgaggcc ttcctatgtg ctgggcggcc
     1921 aaaacatgat cattgctttc agtgatgatg atatccgtga atacatggac attattctgg
     1981 cctacaacat tgaaaacccg gttttgattg ataaatatct gatggggatt gaaatcgagg
     2041 tcgatgccat ctgtgacgga gaggatattc tcattccc
//
LOCUS       DAOTHZ010000165         2044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_65093_length_2044_cov_8.406234, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000165 DAOTHZ010000000
VERSION     DAOTHZ010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65093_length_2044_cov_8.406234"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="P5549_10715"
     CDS             <1..1085
                     /locus_tag="P5549_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW13577.1"
                     /translation="YWIHSRGATQYFCSDTNKIVEYFGEIRPTYMCGVPRLFEKMYAA
                     IYAKMEKAGGIKKNIFDWGIAVGNEYEHARNRGEKISKSLEMKHKLAHKMVLSTVREL
                     LGGRLNFFSAGGAPLAKDIEEFFFAANIFIAQGYGLTETAPMGSCNSPKDFKFGTAGR
                     PIDGNHIKIAEDGEVLIKGDNVFKGYWNKPEATADAMTEDGYFQTGDIGMIDEDGFLK
                     ITDRKKDIIITANGKNIAPQLIESRIGQDYYVEQLVVYGDKQKYLTALIVPAFAALEE
                     YAQDRGIAYQGLSDLIKKPEIIKLYADRIEGQSKDLAHHEKIVRFTLLESPFTIENNE
                     MTPSNKLKRKVINQNYEGLIMKMYKD"
     gene            1569..1718
                     /locus_tag="P5549_10720"
     CDS             1569..1718
                     /locus_tag="P5549_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRW13578.1"
                     /translation="MERNDYMPEDIRDSMDLGTWGFLRTGWWILHIVGITAVGYIGYW
                     IAVSR"
BASE COUNT          637 a          408 c          451 g          548 t
ORIGIN      
        1 cttattggat tcattcccgg ggagcgactc agtatttctg cagtgatacc aacaaaattg
       61 ttgagtattt cggtgaaata cggcctactt atatgtgcgg cgtaccccgg ctgtttgaaa
      121 aaatgtatgc tgccatctat gccaagatgg aaaaggccgg tggcatcaag aaaaatatct
      181 ttgattgggg cattgcggtt ggcaatgagt atgaacacgc ccgcaatcgc ggggaaaaaa
      241 tatccaaatc attggaaatg aagcataaac tggcgcacaa gatggtactg tcgacggtca
      301 gagaactgtt gggcggacga ttgaacttct tctccgccgg gggagcaccg ctggccaagg
      361 atatagagga attcttcttt gctgccaata tctttatcgc ccagggatac ggcttgacgg
      421 aaaccgcacc gatgggttcc tgcaactcgc ccaaagattt taaattcggc acggccggac
      481 gacccattga cggcaaccac atcaagattg ccgaggacgg cgaagttctc atcaaaggtg
      541 acaatgtttt caagggatac tggaacaaac ccgaagccac agcagatgcc atgaccgaag
      601 acggttattt tcagaccggt gatatcggca tgatcgatga agatggattc ctgaaaatca
      661 ccgaccgcaa gaaggacatc atcatcaccg ccaacggcaa aaacattgct ccccaattga
      721 ttgagtcaag aatcgggcag gattattatg tagagcaact ggtggtgtac ggtgacaaac
      781 agaaatacct taccgctttg attgtgccgg cttttgcggc actggaggaa tatgcccaag
      841 accgtgggat tgcctaccag ggactcagcg atttgattaa aaagccagag attattaaat
      901 tgtatgccga ccggattgaa ggacaatcca aagatctggc tcatcatgaa aaaatcgttc
      961 gcttcacctt gctggaaagt ccttttacca ttgaaaacaa tgaaatgacc cccagtaata
     1021 aattaaagcg gaaggtcatc aaccaaaact atgaaggtct tattatgaaa atgtacaagg
     1081 attaattgtg cagaagaatc gtacgacatc agaatgatgg ggaaattgtt acggcaaaag
     1141 caggtgctgt ccttggggca gcacctgctt tcgatttctg ctttttcttt gcagccttta
     1201 gaacggggta ctcagagaat tgatcagaac aaccaatcca atcaccacaa caaaaccgaa
     1261 taaaaatagt ttacacgctc tcatcttatt ggcatcgttc aaaaaacgga ttattttttg
     1321 cccgatacga tccatttttc ttttttttct tattctcttg gtcatcaatc agttctcctg
     1381 gtttaaacca ataaatttgc atgtgtgctg aagatagaaa atggacggac agctgtatgg
     1441 tatcaaatcc gatattatgt attcgaaaag ttcctcgcac aaaatgaggt tttcaataaa
     1501 aaaaactgga aatattttta tacagtttag cttcattcat tctgagcaat actagagata
     1561 aataagatat ggaaaggaat gattatatgc cggaagatat tcgtgacagc atggacctgg
     1621 gcacctgggg atttttgcgg acaggttggt ggattcttca tattgttggt attaccgccg
     1681 ttggttatat cggatactgg atcgctgtat ccagataatc aatttcattt cacccgcatc
     1741 aaactgcata aagcgcatat tggcgggata cttacatgag gattacgcac cggcaaaatc
     1801 ggcatggttt gattaaagaa cgatgccgat tttttcattt ctatcgggat atcgattaca
     1861 ctgaatcact agcaagtgat tcctgtaaat aaaaaacaaa ataatcaggc aaatttgtca
     1921 acatttccat cttatattca tatattaaaa aaaaattaat actttataac agaggtgcca
     1981 atcccgatgt tataaaaaag cgtaaggagg gagaaagatt aatgtcttat tggctatatt
     2041 gtac
//
LOCUS       DAOTHZ010000166         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_67992_length_1979_cov_6.716216, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000166 DAOTHZ010000000
VERSION     DAOTHZ010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67992_length_1979_cov_6.716216"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="P5549_10725"
     CDS             <1..1205
                     /locus_tag="P5549_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRW13579.1"
                     /translation="LPAEPTLPLVDYMQVPKQKIEGTVDFMDIIAKYSKTPLQVEIDM
                     DNDIALFEYTGGTTGLPKGAMLSHYAHLFKPSAACDIMGIGENSRVLTTMPYFNIAGM
                     LFLVGPVLRGATNVLLTQFDPLTVLQAIDKYKVTEWYSAVPMNLAVMAHPDASKYDLS
                     SLQLNMTTSFVVALDEKISKKWEEFTGGCLLLEGAYGLSETHTLDTFCPRQKIKYGSM
                     GIPSYDEEFKIVDFDDKTRELPIGEEGEIVLRNPGSMKGYWNKPEATADTLVDGWVHT
                     GDVGRFDEDGYLYWLGRRKEMIKVSGFSVFPEEVEALINQHPAVLENAVIPIPDPKKG
                     EVIKTFIVLKPDYKDKIAGEEIISWAKESMSSHKVPVYVEFRNELPKQGVKLLRRILR
                     DEEEAKNK"
     gene            1270..>1979
                     /locus_tag="P5549_10730"
     CDS             1270..>1979
                     /locus_tag="P5549_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRW13580.1"
                     /translation="MDTPKNIPDAGDKQAPKIRPELAELMKRNSFLLDENRPQAVERR
                     HQKNQRTVRENIQDFLDEDSFIEYGGLAIAAQRQRRSTEELMVKTPADGMIAGVGTVN
                     SSSFGEEKARCMVLAYDYTVLAGTQGFFNHKKIDRMLKLAHEQRLPLIFFGEGGGGRP
                     GDVDAEGVMIAGLDLSTFGMFGGFSGKAPMVGIVSGPCFAGNAALLGCCDVIIAARNA
                     NIGMGGPVMIEGGGLGTVT"
BASE COUNT          545 a          440 c          513 g          481 t
ORIGIN      
        1 atttgcctgc tgaacctacc cttccgctgg tcgattatat gcaggttccc aaacaaaaaa
       61 ttgaaggaac cgtggatttc atggatatca tcgccaaata cagcaaaact cctcttcaag
      121 tcgagattga catggacaat gacattgctc tttttgaata caccggaggg actaccgggc
      181 tgcctaaagg agccatgctc agccattatg cgcacctgtt taaaccttcg gcagcttgtg
      241 acattatggg gattggagaa aacagtcggg tcctgaccac catgccttat ttcaatatcg
      301 cgggtatgtt attcctggtc ggtccggtat tgcgcggcgc aaccaacgtt ttactgacgc
      361 agtttgatcc cttgaccgtt ctgcaggcga tcgataaata caaagtcacc gaatggtata
      421 gtgccgtgcc gatgaacctg gccgtcatgg cccatcctga tgccagcaag tatgacctga
      481 gcagtttgca attaaacatg accacctcct ttgtcgtcgc gctggacgag aagatctcga
      541 aaaaatggga agaatttacc ggtggctgtc tgctcctgga aggtgcctac ggtctcagcg
      601 aaacccatac cctggatacc ttctgtcccc gccagaaaat aaaatatggc agcatgggga
      661 tcccaagtta tgatgaagag tttaaaattg ttgattttga tgacaaaacc agagaactcc
      721 ctatcggtga agaaggcgaa atcgtgttgc gcaaccccgg ttccatgaaa gggtattgga
      781 acaagccgga agccactgct gacactttgg tggacggatg ggttcatact ggtgatgtgg
      841 gacgttttga cgaagacggc tacctgtact ggctgggaag aagaaaagaa atgatcaagg
      901 tatccggttt ctcagtattc cctgaagaag ttgaggcatt aatcaaccag catcctgccg
      961 ttttggaaaa tgccgttatc ccgattcctg atcccaaaaa gggagaagta atcaagacct
     1021 ttattgttct caagcctgat tacaaagaca aaatcgccgg ggaagaaatc atcagctggg
     1081 ctaaagaaag tatgtcatcc cacaaggtcc ccgtatatgt tgaattcaga aatgaattgc
     1141 ccaaacaggg cgtcaagctg ttacgccgca tcctgcggga tgaagaagaa gcaaaaaaca
     1201 aatagcggat attgtttttg atgttattat ttaataaaac agtattcaaa acaatgggag
     1261 gttttaatca tggatacccc aaaaaacata ccggatgcgg gagacaagca agcacccaag
     1321 attcgaccag aattggcaga attgatgaag cgaaattcat ttttactgga tgagaaccgc
     1381 ccgcaggcag tcgagcgtcg tcatcaaaaa aaccagcgta ccgtccggga aaatattcag
     1441 gattttttgg atgaagacag ttttattgaa tatggcgggt tggccattgc tgcccaacgt
     1501 cagcgcagat caaccgaaga gcttatggtc aaaaccccgg ctgacggaat gatcgccggc
     1561 gtaggcacgg taaacagcag cagcttcggc gaagagaaag cacgttgcat ggttttggct
     1621 tatgattata cggtcttggc cggaacacaa ggttttttca atcacaagaa gatcgatcgt
     1681 atgttaaaac tggcgcatga acagcggctt ccgttgattt tcttcggtga gggcggaggc
     1741 ggcagaccgg gtgatgttga tgctgaaggc gtcatgatcg ctggtctgga cttgtcaaca
     1801 ttcggtatgt ttgggggatt cagtggcaaa gccccaatgg tgggcattgt ttccggccca
     1861 tgtttcgccg gcaacgctgc cctgctgggt tgctgcgatg taattattgc cgctcgcaat
     1921 gccaatattg gcatgggtgg accggtcatg atcgaaggtg gcggtctggg cacagtaac
//
LOCUS       DAOTHZ010000167         1791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_78134_length_1791_cov_7.245392, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000167 DAOTHZ010000000
VERSION     DAOTHZ010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78134_length_1791_cov_7.245392"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1476)
                     /gene="ffh"
                     /locus_tag="P5549_10735"
     CDS             complement(127..1476)
                     /gene="ffh"
                     /locus_tag="P5549_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRW13581.1"
                     /translation="MAFEGLSDRLQEVFRKLRGKGKISEEDVKLAMREVRLALLEADV
                     NYKVVKNFVATVSERAVGQEVIQSVTPGQQIVKIVYDEMTQLMGGSETKLNLSSKPPT
                     VIMAVGLQGAGKTTTVAKLARNLVKQGRRPLMVAADVYRPAAIKQLQVLGEQLNVPVF
                     SMGQGNPVNIAKASMEYAHSNNRDIVIIDTAGRLHINEELMDELVQIQEAVEPHETLL
                     VVDSMTGQDAVTVAESFNEQLGITGLILTKLDGDTRGGAALSVKAVTGRPIKLVGMGE
                     KLDALETFYPDRMASRILGMGDILSLVEKAQENFDEQKAREMEMKIRKQEFSLEDFLD
                     QMQQVKKMGPLQDLLGMIPGMGKQMKGMDAQIDEKEMLHVEAIIKSMTIQERRNPTIL
                     NGSRKKRIAAGSGTRVQDVNRLLKQFEESRKLMKQLTDAGGKKGKRGFMPGMKFPLQ"
     gene            complement(1492..>1791)
                     /locus_tag="P5549_10740"
     CDS             complement(1492..>1791)
                     /locus_tag="P5549_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRW13582.1"
                     /translation="YGALLTDKQRRILKDYYENDFSLSEIADDMNTSRQAVYDLIKRA
                     EHQLMQYEAKLGLLDKLKTTRRQMETVCELLSQEEPDVERLLEARRILQETMELL"
BASE COUNT          358 a          516 c          411 g          506 t
ORIGIN      
        1 tcttggctcc cattctcttt aagcggattt ttgttgccat attgtcacct ccttgttatt
       61 atctctactt tatgtgcatt gcggtctggc ctagaaacct gccgcagcta aagtaagcca
      121 aaaaaattat tgcagtggaa acttcatgcc cggcataaag cctcgtttgc cttttttgcc
      181 gcccgcatcg gtgagctgtt tcatcagctt gcgggactct tcaaactgct taagcaaacg
      241 gttcacatcc tgcaccctgg taccgctgcc cgcggcgatc cgtttcttgc gactgccgtt
      301 aaggatggta ggattgcgtc gttcctggat cgtcattgat ttgatgatgg cttccacatg
      361 cagcatttcc ttttcatcaa tctgggcatc catccctttc atttgcttgc ccataccggg
      421 aatcatgccc agcagatcct gcagtggtcc cattttttta acctgttgca tctggtccag
      481 gaagtcctcc aggctaaact cctgcttgcg aatcttcatc tccatttcgc gggctttttg
      541 ctcgtcgaaa ttctcctggg ctttttccac caggctgagg atgtcgccca tgcccaggat
      601 gcgcgaagcc atccggtccg gataaaaagt ttccagggca tcgagttttt cccccatgcc
      661 caccagcttg atgggccgcc cggttacggc tttgacagac aacgctgcac cgccgcgggt
      721 gtcgccgtcc agtttggtca aaatcagtcc ggtaataccc aattgttcat taaaagactc
      781 ggctaccgtc accgcatcct gtccggtcat ggaatcgacc accagcaagg tctcatgcgg
      841 ttcgaccgct tcttgaatct ggaccagttc atccattaat tcttcattaa tgtgcagccg
      901 tccggcagta tcaatgataa caatatcacg gttgttgcta tgggcatact ccatcgaggc
      961 tttggcgata ttgaccggat tcccctgccc cattgaaaaa accggcacgt tcaactgttc
     1021 gcccagcacc tgcagctgtt tgatggcggc ggggcgatac acatcagcgg ctaccatcag
     1081 cggccggcgc ccttgcttga ccaggttgcg ggccagcttg gccaccgtag tggtcttgcc
     1141 ggcaccctgc agtccgaccg ccatgattac ggtgggcggt ttggaggaca ggttaagttt
     1201 ggtttcgctg ccgcccatca actgggtcat ttcgtcatag acaatcttaa cgatttgctg
     1261 tccgggagta acgctctgaa tcacttcctg acctaccgcc cgctcgctga cagttgccac
     1321 aaagtttttc actactttat aattgacgtc agcctccagc agcgccaggc gcacttcccg
     1381 catcgccaac ttgacgtcct cttcggagat tttgcctttg ccgcgcagct ttctgaatac
     1441 ttcctgcagg cggtctgaca gtccttcaaa tgccacgata tctctccttt tctacagcag
     1501 ctccatggtt tcttgtaaaa tccgccgggc ttccagcaac cgttcgacat caggctcttc
     1561 ttgactgagc aattcgcaga cggtttccat ctgtcggcgg gtcgttttga gcttatccag
     1621 cagacccagt ttggcttcgt attgcatcaa ttggtgttcg gctcttttaa tcaggtcata
     1681 cactgcctgg cggctggtat tcatatcatc agcgatttcc gacaaggaaa aatcattttc
     1741 ataatagtct tttaaaatac ggcgctgctt atcggtcagc aaagctccgt a
//
LOCUS       DAOTHZ010000168         1768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_79494_length_1768_cov_6.259194, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000168 DAOTHZ010000000
VERSION     DAOTHZ010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79494_length_1768_cov_6.259194"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..784)
                     /locus_tag="P5549_10745"
     CDS             complement(137..784)
                     /locus_tag="P5549_10745"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRW13583.1"
                     /translation="MFTGIIEEKGTINRIDKGIQSSRIEIKARTVLEGTKIGDSIAIN
                     GVCLTVTSLGDHHFTADIMAETLAKTNLGQMNSGQPVNLERALRLSDRLGGHLVQGHV
                     DGQGIIAEQKKMDIATILRIKAEQDLLAYIVKKGSIAIDGISLTVADLLADSFTVSLI
                     PHTFANTTLGYKKPGDSVNLETDIIGRYVERLLGNNSQREKSGMSKGFLAENGFI"
     gene            complement(820..>1768)
                     /gene="ribD"
                     /locus_tag="P5549_10750"
     CDS             complement(820..>1768)
                     /gene="ribD"
                     /locus_tag="P5549_10750"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRW13584.1"
                     /translation="AEVHALRQAGAMAAGATIYVSLEPCCHHGRTPPCADALIKAGIK
                     RAVIATLDPNPLVAGKGMQKLLDAGIEVEIGVLEAEACKLNEGFFQYIKTKRPLVAIK
                     TAMTLDGKIAAKSGDSRWITAEDTRNYVHQLRGVYDAILVGIGTVLQDDPRLNVRLEN
                     CDYSDPVRVVIDSHLEIPMYSQIVSTSRQQRSIIFCAADADQARQTRLEEAGCEVQRV
                     NSCEGKLQLEEVLDRLGEMKLCTLLVEGGGTINAAFLENGLADKVYWFIAPKIIGGQA
                     APTPVEGRGFDFMKEALELESFEITRFSRDILLTGLIQK"
BASE COUNT          389 a          471 c          407 g          501 t
ORIGIN      
        1 gtgtattttt catataggga cgcctctctt tcaattgttt tgtgccatct ttgcgggatt
       61 cagcacaatt caattgtatt ggtgggcgtc tcttttttca aagactgttt ttaaggatta
      121 caggaatgct ccttacttat ataaagccat tctcagccag aaatcccttg ctcatgccgg
      181 atttttccct ttgagaatta tttcccagca atctttccac ataacggccg ataatgtctg
      241 tttccaggtt gacggaatca ccgggctttt tatatcccag ggtcgtattg gcaaaagtat
      301 gcgggatcag cgatacggta aaactgtcgg ccagcaggtc ggcaaccgta agactgatgc
      361 cgtcaatggc aatggaacct ttttttacaa tgtaggccag caggtcctgc tcggctttga
      421 tgcgcaagat ggtggcaata tccatctttt tttgctcggc aatgatcccc tgcccatcaa
      481 catgaccttg taccagatga ccgcccagac gatcgctgag gcgcaaagct ctttccaaat
      541 tgaccggctg tccgctgttc atctgcccca aattggtttt ggccagggtc tcggccatga
      601 tatccgctgt aaaatgatgg tctcctaaag aggtaaccgt aagacatacg ccattgatgg
      661 cgatactgtc tccgattttg gtaccttcca ggacagtgcg ggcttttatt tcgatacggg
      721 acgattgtat tcctttgtcg atgcggttaa tggttccttt ttcttcaata attccggtaa
      781 atatttttat cactcctgtt tgggacgaag tatcccctgt tatttttgaa tcagtccggt
      841 gagcagaata tcacggctaa atctggttat ttcgaacgat tccagctcca gcgcttcttt
      901 catgaagtca aacccccggc cttcgacagg tgtaggcgca gcttgtccgc cgataatctt
      961 gggagcaata aaccagtaca ctttatcagc caggccattt tccaaaaaag cagcgttgat
     1021 ggttcctccc ccttccacca gcaaggtgca aagtttcatc tcccccagac gatccagcac
     1081 ttcctccaac tgcagcttgc cctcgcagct gttgacccgc tgcacttcgc agccggcttc
     1141 ttccaaacgg gtttggcggg cctggtcggc atcggcggcg cagaagatga tgctgcgctg
     1201 ttgccggctg gtggagacga tttgactgta catggggatt tccagatgac tgtcgatgac
     1261 gactctgaca ggatcactat aatcacagtt ttccagcctt acgttgagcc ggggatcatc
     1321 ttgtaaaacc gttccgatcc cgaccaggat ggcatcataa acgccacgca attgatgcac
     1381 atagttgcgg gtatcttcag ctgtaatcca gcgggagtct ccgcttttgg ccgcgatttt
     1441 tccatccagt gtcatagccg tcttgatggc gaccaatggt cgtttggttt taatgtattg
     1501 aaaaaatcct tcattgagct tgcaggcttc cgcttccaaa acccctattt ccacttcaat
     1561 ccctgcatcc agcaattttt gcatgccttt cccggccacc agcggattag ggtccaaggt
     1621 agcaatcacc gctcttttaa taccggcctt gatcagcgcg tctgcacacg gcggggtacg
     1681 accatgatga cagcagggct ccaggctcac ataaatggtc gcccctgctg ccattgcacc
     1741 tgcctgccgc agcgcatgta cttccgca
//
LOCUS       DAOTHZ010000169         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_79677_length_1765_cov_6.512865, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000169 DAOTHZ010000000
VERSION     DAOTHZ010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79677_length_1765_cov_6.512865"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="P5549_10755"
     CDS             <1..549
                     /locus_tag="P5549_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRW13585.1"
                     /translation="SMDILISSNLERLLYELTGHDAAKVKRWMEELKNQGSYQVDEEV
                     LQKIQQLFWADCGNDRETMDAIKQVWEKHHYLLDTHTAIAWCVYQRYVSIHGDTTPTV
                     IASTASPFKFAPSVAEALLGAEAIVGQDEFASLNLLSHHTGIEIPAAIRELEQKPILH
                     QTSTSREEMQATVCKMIFGDCP"
     gene            complement(553..1761)
                     /locus_tag="P5549_10760"
     CDS             complement(553..1761)
                     /locus_tag="P5549_10760"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRW13586.1"
                     /translation="MKYLLLLADGMADYKYDVLQDKTLLEYAHTPNMDKLAPLSQLGM
                     ARTVPEGYPPGSDVANLAVMGYDPRRYYTGRSPLEAVSLGVAMGEQDLSLRCNLVTLS
                     DAPDYRDKVMLDYSAGEISTAESAQLIDAIGKALGSDEQSFHPGISYRHLLLWRNALG
                     KSLQLTPPHDISDRVIGSYLPQGDDSQILLQMMQASQAILTNHPVNLARLEQELPPAN
                     SIWLWGEGSRPAMDSFADKYGLQGSVVCAVDLVKGLGLCAGLTPVQVEGATGAIKTNF
                     AGKARAALDELKRGQDFVYLHIESPDEAGHQGEMESKIWAIEQIDQQVLGLILAEMDN
                     FDDLRLLIMPDHPTPIRIRTHTSDPVPFMLYDKNHPQASSARAFHETTAAEGIFFADG
                     YQLMDYFIKG"
BASE COUNT          429 a          486 c          462 g          388 t
ORIGIN      
        1 tccatggata ttctcatctc ctccaacctg gagagactgc tctatgaatt aaccggccat
       61 gatgccgcaa aagtaaagcg ttggatggag gaactcaaga accagggcag ttatcaggtg
      121 gatgaagaag ttctgcaaaa aattcagcag ttgttctggg cggattgcgg caatgatcgt
      181 gaaacgatgg atgcgatcaa acaagtctgg gaaaagcatc attatttgct ggatacccat
      241 acggccattg cctggtgtgt ttaccagcgc tacgtttcca tacacggaga tacgaccccg
      301 acagttattg cttcgaccgc cagtccgttt aaatttgcgc cgagcgtggc cgaagcactg
      361 ctgggcgcag aggcgatagt aggccaggac gaatttgcca gcctgaatct gctttctcat
      421 cataccggca tagaaatccc ggctgccatc cgggaactgg agcaaaaacc gattctgcac
      481 cagaccagca ccagccggga agagatgcaa gctaccgtat gcaaaatgat ttttggggac
      541 tgtccataaa aatcatccct tgataaaata atccatcagc tgataaccat ccgcaaagaa
      601 gattccctcg gcggcggtcg tttcatggaa cgcccgggcg gaggaggctt gcgggtggtt
      661 tttgtcatag agcataaacg gaaccggatc cgacgtgtgg gtcctgatgc gaatcggagt
      721 gggatgatcg ggcatgatca acagccgtag atcatcaaaa ttatccattt ccgcaagaat
      781 cagccccagc acctgctgat caatctgttc aatcgcccag attttacttt ccatttcgcc
      841 ctgatgaccg gcttcatcag gtgattcgat atgaagataa acgaaatcct ggcctctttt
      901 cagttcatcc aaagcggctc gtgccttgcc ggcaaagttc gttttaatcg caccggtggc
      961 accttccacc tggaccgggg tcagaccggc gcacaggccc agacccttga ccaggtcgac
     1021 cgcacaaacc accgaaccct gcaacccgta tttgtctgca aagctgtcca tggccggccg
     1081 gctgccttcg ccccacagcc aaatggagtt ggctggagga agctcctgct caaggcgggc
     1141 cagattgacc ggatggttgg tcaaaatggc ctgactggct tgcatcatct gcagcaggat
     1201 ttgactgtcg tcaccctgag gcaggtaaga accaatgacc cggtcggaaa tgtcatgggg
     1261 aggcgtcaac tgcagggatt tgcccaaggc atttctccac aacagcagat gacggtaact
     1321 gataccgggg tggaaactct gttcatcgct gcccagcgct ttgccgatcg catcaatcag
     1381 ttgggccgat tctgccgtgc tgatctctcc ggcactgtag tccagcatca ccttgtccct
     1441 gtagtcgggc gcatcggaaa gtgttaccag gttgcagcgc aggctcagat cctgctcgcc
     1501 catcgccaca cccaggctga ctgcttccag gggagagcga cccgtataat accggcgcgg
     1561 atcataaccc atcaccgcca aattggcgac atcactgccg gggggatagc cttccggcac
     1621 ggtgcgagcc atgcccagct gcgacaaggg tgccagtttg tccatgttcg gcgtatgggc
     1681 atactccagc aaggttttgt cctgcagaac atcgtattta taatcggcca tgccatcagc
     1741 cagcaacagc aaatacttca tatac
//
LOCUS       DAOTHZ010000170         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_82245_length_1726_cov_9.484141, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000170 DAOTHZ010000000
VERSION     DAOTHZ010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82245_length_1726_cov_9.484141"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="P5549_10765"
     CDS             complement(<1..161)
                     /locus_tag="P5549_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRW13587.1"
                     /translation="MLFNHVFKALSDSTRRQILNMLQMGDLTAGDIAEQFAMTKPSIS
                     HHLNILKQA"
     gene            450..>1726
                     /locus_tag="P5549_10770"
     CDS             450..>1726
                     /locus_tag="P5549_10770"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRW13588.1"
                     /translation="MKNLYITEKPSVADSFASVLGVEISKSDRSRGFAENDNAIISWC
                     YGHLVTMAYPEAYDPAYKSWRVEHLPIIPQEYKYEIVNNAGAKKQFEVIKKLMHREDV
                     SMIYSCTDSGREGEYIFRLVYQESKTDKPAKRVWISSQTEDAVRRGIEEAKDIHAYDP
                     LGRAAYCRAKEDWLFGMNFSRIYTCRYGKELGARLKEDKRSVIAIGRVMTCVLGLVVD
                     RELEIRDFVPKVHYGVMASFLSQTSGIAYKGKWQPTKNKGTKKDDQSGEEEKYFSQEE
                     AQAIIDKINGHEATVKKVEIKVKKEPPPLLFNLAELQSEANKKFKLPVDKTLEITQSL
                     YEKKLISYPRTDSRVLSTDIISEIPKVLNGLYKNAAFKDAVIRIKDFGKLAIDKSAKR
                     FVDDSKVTDHYAIIPTYVATDLSKLNEDSRNVY"
BASE COUNT          521 a          333 c          428 g          444 t
ORIGIN      
        1 ttggcctgtt tcaggatatt gagatgatgc gatatactgg gtttggtcat ggcaaattgc
       61 tcggcgatat ctccggccgt gagatcaccc atctgcagca tattcagaat ttgccggcgg
      121 gttgaatcgg atagagcttt aaacacatga ttaaacagca taacatttca ccttcatgtt
      181 gattgttaga tatttagaac atcatctaaa tatctagcaa tagtttactg cattttttaa
      241 aaaaagcaat agtgatctat gtttgatgtt gttaccatga gctactgtga ttactgtgtt
      301 tttatttgat aatcagatga aatgctttta agaatcaagc agtataactt gcaggaaaac
      361 gggtttgttt ttgcgaatct gctatgatag cagtggttac agaaaaattt atctgcatga
      421 ttcgtttttg gacgcgagga aaaacattga tgaaaaattt gtatataact gaaaaaccat
      481 cggtggcgga cagctttgcc agtgtattag gggttgaaat atccaagtcc gatcggagcc
      541 ggggatttgc agaaaacgat aatgccatca ttagctggtg ctatggtcat ctggtgacga
      601 tggcctaccc ggaggcctac gatccggcct ataaatcatg gcgggtggag catctgccga
      661 tcataccaca ggaatataaa tatgagatcg ttaataatgc cggtgccaaa aaacaatttg
      721 aagtgatcaa aaaactgatg caccgtgagg atgtatccat gatttactcc tgtaccgaca
      781 gcggacgcga aggggagtat atatttcgcc tggtttatca ggaaagcaaa acggataaac
      841 cggccaaacg agtctggata tcctcccaga cggaagatgc ggtcagaaga ggcatcgagg
      901 aagccaagga tattcatgcg tacgatcccc tgggccgggc agcctattgc cgcgccaaag
      961 aggattggct gtttgggatg aatttcagca gaatttacac ctgtcgctat ggcaaggagt
     1021 tgggggcccg gctaaaagag gacaagcggt cggtcattgc cattggcaga gtgatgacct
     1081 gtgttctggg gttggtcgtc gatcgtgaat tggaaatcag ggactttgtg cccaaagtcc
     1141 attatggggt gatggccagc tttttgtctc aaacgagcgg tattgcctat aagggaaaat
     1201 ggcagcccac aaagaacaag ggcaccaaaa aggacgatca atctggtgaa gaggaaaaat
     1261 attttagcca ggaagaagcg caggcaatta ttgacaagat aaatggacac gaagccaccg
     1321 tcaagaaagt cgagattaaa gttaaaaagg agccccctcc gctgcttttt aatctggctg
     1381 aattgcaatc ggaggccaac aagaaattca aacttccggt tgataaaacc ttggaaatca
     1441 cccagagcct ctatgagaaa aaactgatct cttatccgag aacagacagt cgggtattat
     1501 ctaccgatat tattagtgaa attcccaaag ttctgaatgg cctgtacaag aatgccgcct
     1561 tcaaggacgc agtcattaga attaaggatt ttggcaagct ggcgattgat aaatcggcca
     1621 agcggtttgt agatgacagc aaggttactg accactatgc aatcatcccc acctatgtgg
     1681 cgactgatct gtctaaattg aatgaagaca gccgtaacgt ttacag
//
LOCUS       DAOTHZ010000171         1692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_84633_length_1692_cov_6.775809, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000171 DAOTHZ010000000
VERSION     DAOTHZ010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84633_length_1692_cov_6.775809"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..824
                     /locus_tag="P5549_10775"
     CDS             57..824
                     /locus_tag="P5549_10775"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRW13589.1"
                     /translation="MQIHILASGSTGNAALIAMGGRKILIDAGISARRIEKGLAEVGF
                     DASQLDAIFVTHEHSDHIQGLDVLVRRYQIPVYTRPATWEKIPCRDKLPRECCRELGS
                     AINLGRVKIESFAIPHDAADPVGFCVLEAQYKTVLATDLGRITPTVETALAYADILIL
                     ESNHDPVMLKEGPYPSYLKKRISGPQGHLSNQEAGALLARVPHKDKLQVFLAHLSQQN
                     NFPHLAMSTVGSILQQKGCSGIQLQRTYPAATASLVS"
     gene            complement(939..1460)
                     /locus_tag="P5549_10780"
     CDS             complement(939..1460)
                     /locus_tag="P5549_10780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRW13590.1"
                     /translation="MPRNPAVTIEMESGAQIVIELYPEIAPNTVRNFVALVSSGYYDG
                     VIFHRVIPGFMIQGGCPQGTGTGGPGYSIKGEFSANGFANPLQHDRGVISMARTSMPN
                     SAGSQFFIMTQKSPHLDGQYAAFGKVILGLEEVDRIVSVDRSRNDKPFEDQRMAKVTV
                     DTFDTVIGPVEKN"
BASE COUNT          416 a          409 c          453 g          414 t
ORIGIN      
        1 tgcagcccag tgataattta actggcagtt tgattaataa cacaaggagc aatgacatgc
       61 agatacatat tctggccagc ggcagtactg gcaatgctgc tttaatagcc atgggcgggc
      121 gcaagatcct gattgatgcc ggcataagcg cacgccggat tgaaaaaggg ctggcggaag
      181 ttggttttga tgccagtcag ttggatgcga tattcgtgac ccatgaacat agtgatcata
      241 tccagggctt ggatgtgttg gtgcggcgtt accagatacc ggtttatacc cgtccggcaa
      301 cctgggaaaa gattccttgc cgggacaaac tgcctcgcga gtgctgccgt gagctgggct
      361 cggctataaa tttgggaagg gtcaaaatag agtcttttgc cattccacat gatgcggctg
      421 atccagtcgg tttctgtgtc ttggaggctc aatataaaac cgtactggct accgatctgg
      481 ggcgaataac gcctacggtg gaaactgcgc tggcctatgc cgacatattg attctggaat
      541 cgaatcatga tccggtgatg cttaaagagg ggccttatcc atcttatttg aagaagcgca
      601 tcagcggtcc gcagggacat ctttccaatc aggaagcggg tgccctattg gcgagggttc
      661 cgcataaaga caagctgcag gtattcctgg cgcacctgag tcagcaaaac aattttccgc
      721 atttggcaat gagcacggtt gggagcatcc tgcagcaaaa aggctgcagc ggaatccaat
      781 tgcagcgtac ttacccggcg gcaacggcca gcctggtgag ttaaaacaga gttgcttctg
      841 ccgggaccgg gcgatagtct gttcctggaa ggctgcaggc aataaaaaag ctgtatccag
      901 agcctagcca ggatacagct tttttcgccg ggaggcagtt aattcttttc cacgggaccg
      961 atgactgtgt caaaggtatc cacggtgact ttcgccatgc gctgatcctc aaatggtttg
     1021 tcgttgcggc tgcggtccac actgacgatc cgatcgacct cttccaggcc taaaatcacc
     1081 ttgccgaaag cggcgtattg tccatccagg tgaggtgatt tttgcgtcat gataaagaac
     1141 tgggaaccgg ctgagttagg catggaggtt ctggccatgg agataacccc gcgatcatgc
     1201 tggagcggat tggcaaatcc gttggcagaa aactcgcctt ttatactgta gcccggtccg
     1261 cccgtcccgg ttccctgcgg acatcctcct tggatcataa agccgggaat gacacggtga
     1321 aaaatcactc cgtcgtaata gccggaactg accagggcta caaaattgcg gaccgtgttg
     1381 ggcgcgattt caggatataa ttcaatcacg atttgagcgc cgctttccat ttctatggta
     1441 actgcgggat ttcggggcat taaaaatcat cctttccaaa cgttattcag tgcttgcgtg
     1501 acaatgccaa cagaatacag caaaaaaaga cgatgggcaa gcccgagtgt ttaatttccc
     1561 aggaacattc tcttaacaca ttcttttaac ttgacggagt ctcttttttc ccggccttga
     1621 actgactgat aatgccgggc aactgagcca tgacgctttc catggagttt ttatggccca
     1681 gcggcaacac ac
//
LOCUS       DAOTHZ010000172         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_90316_length_1616_cov_7.695708, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000172 DAOTHZ010000000
VERSION     DAOTHZ010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90316_length_1616_cov_7.695708"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1616)
                     /locus_tag="P5549_10785"
     CDS             complement(<1..>1616)
                     /locus_tag="P5549_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRW13591.1"
                     /translation="DLIAFLADLQKQGFVITPLQDKNRDGERFLLREIDPFTFLGVFN
                     RGIRDEQRFAIITQMKRHFQLQSQVPSDFSGIPVLNNQKSWLFPYQESRKPDDIPRLW
                     RVFRLALQKNPFQNRDFLTAFDEALTVSNVNINLTMGLFWVRPHTFLSLDSQIRNYLN
                     ITMPKGGLTAKFYADIVRKFATQERSFLELSLSAWESGRKPTDNGNHPISPPPTPRSK
                     DVTYWLVGAYWDDRDPQDQTARFLDEGIWENGYLDRYLEDVRAMKVNDKIAIKAATTQ
                     RKNLPFDGQGHTVSLMIIKAVGTIVANRGDGRTVEVEWEPDFAEKSWYFYTNRSTVWR
                     LRTDDNYNLKPYSEKLIEFIWGGQPQDYAWFAAQWWGEQKSGKASLQPLPDGSHTPYG
                     VEDMLAAGVFLTEAQIHQMLERLSTKKAMILQGAPGVGKTFIARKLAYALMNEVDNGR
                     LEMVQFHQSYAYDDFVRGYRPLPGKAGSFGLQNGVFFEFCQKAAQDPDREYVFIIDEI
                     NRGNLSLIFGELLMLIEGDKRGPEFAVPLV"
BASE COUNT          372 a          411 c          442 g          391 t
ORIGIN      
        1 tagaccaggg gcacggcaaa ctccgggccg cgcttgtcgc cttcgatgag catcagcagt
       61 tcgccaaaaa tcaggctcag gttgccccgg ttgatttcgt cgatgatgaa cacatattcc
      121 cggtcagggt cttgggcagc cttttggcag aactcaaaga aaacgccgtt ttgcaggcca
      181 aagctgcctg cttttcccgg cagcggccga tacccgcgca caaagtcatc gtaggcgtag
      241 gattggtgga actggaccat ctccaaacgg ccgttatcca cctcattcat cagtgcgtag
      301 gccagtttgc gggcgatgaa ggttttgccc acacccggcg cgccttgcaa gatcatcgct
      361 ttcttcgtac tcaggcgttc cagcatctgg tgaatctgcg cttcggtcaa aaagaccccg
      421 gcggccaaca tatcttcaac gccgtagggt gtatggctgc catccggtaa tggttgcagg
      481 gaggctttgc ctgacttctg ctcgccccac cactgggccg caaaccaggc ataatcttga
      541 ggttgcccgc cccagatgaa ctctatcagc ttttcactat acggcttgag gttgtaattg
      601 tcgtcggtac gcaaccgcca gacggtgctg cgattggtgt agaagtacca ggatttttcc
      661 gcaaagtccg gttcccactc gacctcaacg gtacggccgt cgccccgatt ggccacaatt
      721 gtcccgacag ctttgatgat catgagcgat acggtgtggc cttgaccgtc gaagggcagg
      781 ttttttcgct gggtagtagc agccttgata gcgattttgt cattcacctt catggccctt
      841 acgtcttcca gatagcggtc gaggtagcca ttttcccaaa tgccttcgtc gagaaagcgg
      901 gctgtttgat cttgggggtc gcggtcatcc cagtaagcgc caacgagcca ataagtaaca
      961 tctttgctcc tgggcgttgg cggaggtgaa atagggtgat tgccattatc ggtcggtttg
     1021 cggcctgatt cccaggcaga aagagagagt tcaagaaagg accgttcctg agtggcaaat
     1081 ttccggacaa tgtcggcata gaacttggct gtcaagccac ccttgggcat ggtgatgttc
     1141 agataattgc ggatctgact gtccagactc aggaaggtat gaggacggac ccaaaacaaa
     1201 cccattgtca ggttgatgtt gacgtttgag acagtcaatg cttcatcaaa ggcggtcaaa
     1261 aaatcccgat tctgaaaagg atttttttgc agagcaagtc ggaaaacacg ccataaacga
     1321 ggaatgtcat ccggttttct actttcctga tagggaaaga gccacgattt ttgattgttg
     1381 agaactggta tgccggaaaa gtcagacggc acctggcttt gaagctgaaa gtggcgcttc
     1441 atctgcgtga taatggcaaa acgctgttcg tcgcgtatgc cccggttaaa aacgcccaag
     1501 aaggtgaagg gatcgatttc acgcagcaga aatcgttcgc catcgcggtt tttgtcctgc
     1561 aatggggtaa tcacgaaacc ctgcttctgt aagtcggcta ggaaggcgat caggtc
//
LOCUS       DAOTHZ010000173         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophomonas sp. isolate AMR_MDS_3038
            NODE_98676_length_1519_cov_5.165984, whole genome shotgun sequence.
ACCESSION   DAOTHZ010000173 DAOTHZ010000000
VERSION     DAOTHZ010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868062
            Sequence Read Archive: SRR5007144
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophomonas sp. (wastewater metagenome)
  ORGANISM  Syntrophomonas sp.
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Syntrophomonadaceae; Syntrophomonas.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 12:56:52
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,159
            CDSs (total)                      :: 2,122
            Genes (coding)                    :: 2,109
            CDSs (with protein)               :: 2,109
            Genes (RNA)                       :: 37
            tRNAs                             :: 33
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Syntrophomonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98676_length_1519_cov_5.165984"
                     /isolate="AMR_MDS_3038"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053627"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2014-12-17"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="P5549_10790"
     CDS             complement(<1..824)
                     /locus_tag="P5549_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRW13592.1"
                     /translation="MKKRLALILVICLSLTLLIAGCGNKADQTTPPEQQQTGDTSFQD
                     IKDQGYFTLGLDDAFPPMGFREEGTNEIVGFDIDLAKEAAKRMGVEVKFQPVVWDTVI
                     EELNNGNIDVIWNGLTITPARQEQIAFTKPYISDRQIIVVQKGSAITNKAGLAGKKVG
                     IQAASSAIEAVEKDAATFESLGELVQFDNNNDALLDLKSGRLDAVVVDEVVGRYYLAK
                     HAEDYDVMEENFGSEDFGIGVRKTDKTFLEELQKALDAMKADGTSAEISKKWFGED"
     gene            complement(1019..>1519)
                     /gene="nth"
                     /locus_tag="P5549_10795"
     CDS             complement(1019..>1519)
                     /gene="nth"
                     /locus_tag="P5549_10795"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRW13593.1"
                     /translation="TDEQVNRVTAALFREANTPEALAAMEQSELEERIKGVGLYKNKA
                     RSIKNLSLMLLEKYDGEVPGDYDALLQLPGVGTKSANVVMAVGFDRPGLGVDTHVQRV
                     TNRLGIVQEKTPEKTEKALKALLPEKRWSISHHLFIFHGRQVCKARRPDCENCCVEDL
                     CAKVFR"
BASE COUNT          324 a          418 c          352 g          425 t
ORIGIN      
        1 atgtcttcgc cgaaccattt cttggagatc tctgcggaag ttccgtcagc tttcatggca
       61 tccaacgctt tttgcagttc ttccaagaaa gtcttgtcag tctttctgac acctatgcca
      121 aaatcttccg agccgaaatt ttcttccata acatcatagt cttcggcatg tttggccaga
      181 tagtaacgtc ctacgacttc atcaaccaca actgcatcga gccgaccgga tttgagatcc
      241 agcagggcat cgttgttgtt gtcgaattgc accaattccc ccagagattc aaacgtagcg
      301 gcatcttttt ccaccgcttc gatggcgctg ctggctgcct ggataccgac tttcttgccg
      361 gccagacccg ctttattggt gatggcggag cccttctgca cgacgatgat ctgacgatcg
      421 gaaatgtaag gtttggtgaa agcgatctgc tcctgacggg ccggagtgat ggtcagacca
      481 ttccagatca cgtcaatgtt gccgttgttc aattcctcaa taaccgtatc ccataccacc
      541 ggctggaatt tcacttccac gcccattctc ttggcggctt ctttggccag gtcaatgtca
      601 aaaccgacga tttcattggt gccttcttcc cggaatccca tgggcgggaa cgcatcatcc
      661 aaaccgaggg tgaaataacc ctgatctttg atgtcctgga aggaagtgtc gcctgtctgc
      721 tgctgttcgg gcggggtggt ctgatccgct ttgttgccgc agccggcgat cagcagggtc
      781 agggataaac agattaccag aataagtgcc agtcttttct tcaaaacaaa ttccccctta
      841 ataatttact cagcatttct attttactgt aattgaacaa acattgtaaa tatggcaatt
      901 atcttccgta taaaaatgtt atcttggctg cgtttgtact ttaatccgtt ttgcctgtca
      961 ggttttcagt ggtttatgcc ttcgtccgct gggagccggt ctcctccaag gaaacttatt
     1021 accggaaaac cttggcgcac agatcctcta cgcagcaatt ttcacagtcc ggacggcggg
     1081 ctttgcagac ctggcgccca tggaagatga acaaatgatg actgatgctc cagcgctttt
     1141 ccggcagcag tgctttaagg gccttttcgg ttttttccgg ggttttctcc tgaacgattc
     1201 ccagacggtt ggtcacccgc tgcacgtggg tatccacacc cagtccagga cggtcaaaac
     1261 ctaccgccat gaccacattg gcgctcttgg taccaactcc gggcagctgc agcagggcgt
     1321 cataatctcc cggtacttca ccgtcatact tttccaacag cattagtgaa agattcttga
     1381 tactgcgggc tttgtttttg taaagcccga cccccttgat cctttcctcc aactcgctct
     1441 gctccatggc agccaaagcc tcgggggtgt ttgcttcccg gaacagtgca gccgtgaccc
     1501 ggttgacctg ctcgtctgt
//
